LOCUS       DAOSMP010000001        79266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_324_length_79266_cov_21.407103, whole genome shotgun sequence.
ACCESSION   DAOSMP010000001 DAOSMP010000000
VERSION     DAOSMP010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 79266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 79266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79266
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_324_length_79266_cov_21.407103"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(325..717)
                     /locus_tag="PKX09_00005"
     CDS             complement(325..717)
                     /locus_tag="PKX09_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HOU16250.1"
                     /translation="MTILGNILWIFLGGGILIFFFYLFGGVVLCLTIIGIPFGMQCIK
                     LSVLGLMPFGQKIVYKASEPGCLSTLMNIIWLLFGGLELAVVHLVFAALCAITIIGIP
                     FARQHIKLLSLALTPFGREVQPIQKDRT"
     gene            complement(856..1287)
                     /locus_tag="PKX09_00010"
     CDS             complement(856..1287)
                     /locus_tag="PKX09_00010"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HOU16251.1"
                     /translation="MSINETKIVVCPHCQHEQSVKIWQSLNTALDKEVKKELFEGKIN
                     LLVCENCQQKTFIPVPFLYIDPERQIAVHYFPTAVIRDEKFLQQFNIDGTYTGGESTG
                     LEVPDYMRYLHIVFNMDELVTFIVFREVLFDFHRTGKVLLD"
     gene            complement(1311..3437)
                     /locus_tag="PKX09_00015"
     CDS             complement(1311..3437)
                     /locus_tag="PKX09_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOU16252.1"
                     /translation="MNFRKQLRTSISAISLALAIWLSGCPSRPLTPPTAKIEPKTTII
                     HSDTLIDNYAWLREKSNPEVIQYLEDENAYTEAIMKPTRRFQKKLYKEMLARIKETDE
                     SVPVKIDSFYYYNRMVKGNQYPIYCRKKGSLDAKEEILLDQNRLASGQKYCSIGEFKV
                     SPDHSLLAFAVDFNGSEKHMLVFKDLINGQMLPDTILDVSYGLEWANDNRTVFYTTLD
                     TILRPDKLWRHQLGQTPENDVMVFHEPDEKFYLGIAKTRSHGYLILTLGSQITTEVHY
                     LSADQPDGRFRLFCPRKTGVEYYIDHHSDKFYILTNENAINFRLLETPIARPEKHNWQ
                     EKIPHRPNVMLTGIDLFCDHLVVYERENGLEKIRVENLNTGVMHYVDFPEPVYTIDGD
                     NNPDYNTKILRFRYTSLVTPNSVYDYDMESQTRELKKQDEVLGGYDQTQYQSERIYAI
                     AGDGVKVPISLVYKKGMKKDGSNPLLLYGYGAYGIPSEARFSSIRLSLLDRGFIYAIA
                     HVRGGGELGRQWYEDGKLLRKRNTFTDFIACAEMLIAEKYTSAEKLVINGGSAGGLLM
                     GAVVNMRPELFEVVVAEVPFVDLINTMLDRSIPLTVIEWEEWGNPHLEKYYNYMKSYS
                     PYDNVGPKAYPHMLITAGLNDPRVAYWEPAKWTAKLRATKTDDNVLLLKTDMGKGHFS
                     ASGRYDYLKEVAFKYAFIFAKLGIRE"
     gene            complement(3450..3938)
                     /locus_tag="PKX09_00020"
     CDS             complement(3450..3938)
                     /locus_tag="PKX09_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007984056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU16253.1"
                     /translation="MAQNNAEILIRSARLSDADDLVAFNRAMAQETENRILSAEVISA
                     GVRNLFQHPEWGFYIVAESDGRMVGCLMITYEWSDWRNGLFWWIQSVYVPPEFRRRGI
                     YRKMYDYVQNAALKVGNVCGLRLYVERDNRIAQKTYRKMGMSKTDYILFETTELAKRT
                     NK"
     gene            4222..5286
                     /gene="rlmN"
                     /locus_tag="PKX09_00025"
     CDS             4222..5286
                     /gene="rlmN"
                     /locus_tag="PKX09_00025"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOU16254.1"
                     /translation="MFLMRTSAKINLKGLNLEEITSLLAALGEKPYRARQIYQWLYQK
                     RATDWSQMSNIPLALRTKLAERFELSSVRLQNLVHSRIDGSVKFLFELTDGNLIEAVY
                     MPEDRRRTVCLSSQVGCPIGCAFCATGVMGLKRNLTAGEIIDQLLFINANMPVPATNV
                     VFMGMGEPFLNYENVIRAARIFNSELGPELSARHITISTCGVIPGIERFTAEDQRFKL
                     AISLNATTDEQRDKIIPINRKYPIKELLGVAKNYTRVSRRRVTFEYILIAGLNDTIKD
                     AQRLVKLLSPIPCKLNLIPYNDNQQFPYRAPSEEHLNEFIQEIYQAPFAVTVRRSKGL
                     DIAAACGQLYVTEQNKRNLR"
     gene            complement(5470..5775)
                     /locus_tag="PKX09_00030"
     CDS             complement(5470..5775)
                     /locus_tag="PKX09_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16255.1"
                     /translation="MDVWISYLTSHPIIGVALTAALVLFIIMVIARLLKWAVISFIIL
                     ALAVGLTYKIAQPKKIIDRIQEDVESVSKNGAEKASDKIQKSAEEAVKKVKESLKKE"
     gene            complement(5792..6316)
                     /locus_tag="PKX09_00035"
     CDS             complement(5792..6316)
                     /locus_tag="PKX09_00035"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOU16256.1"
                     /translation="MTNLELNILGIVSEGATHAYNIEKIIAERGIRERTNVGFSTIYA
                     VLNKMQKIGLLMSNLEPQKGLPSRRIYSITPKGRQIFNEEIFKALSQPQHQPSLFETG
                     LTYSGVLNKEALREALTLYDTELGRQIQNKVHELTALSNTNDQVQRALLTRPLVILQA
                     ERKWIRELMGLLLT"
     gene            complement(6378..9230)
                     /locus_tag="PKX09_00040"
     CDS             complement(6378..9230)
                     /locus_tag="PKX09_00040"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOU16257.1"
                     /translation="MKVHIPKSIVVAALFTTMIACSTSELTRKIPPTPPSLSQSLELD
                     SEIALDPKITKGKFDNGLTYYIRQNEKPERRAELRLVVNAGSVLEEEDQRGLAHFLEH
                     MAFNGTARFKKQEIINFMELAGMPFGSHLNAYTSFDETVYMLTVPTDRDSLLEKGFQI
                     LADWASQISLEDEEIDKERGVIREEWRLGRGAEMRIQEKEFQVIFWGSLYAERHPIGQ
                     IAVIDTFHYDTLRKFYRDWYRPDLMAVIAVGDFDEDRVKNLIEQNFIGLKSPADAPPR
                     PIPPVPDHTETLFSIQTDPELTRNQVDLLIKLPRTEQKYIRDYRQSIVENLYNSMLNE
                     RLGELTKTAEPPFLGAASQKGRLVRSKDCYSIGAVVKDNGIEEGFTAILTELKRVREF
                     GFTETELKRAKSTALRNMEQIYRERDKIYSRSFASEYIRNFLDNEPVPGIELEYEFYK
                     KYVPEIALPEVNVLAKEWVTDINRVVLVSAPEKPDLKIPTETELREIFEKVERQEVTA
                     YIDEVAESDLVRQLPPPGRILQETLIDSLDLTELILSNGVKVIIKPTDFKNDEILLSA
                     YSPGGTSLVPDSNYVAAISSVPIVTESGLGEFNSVMLSKKLAGKVVSVQPSLEELTEN
                     FSASASPEDLETMFQLIYLYFTAPRKDSTAYLSYLEKLKNSIVNRAADPFAAFSDTIR
                     VTLSQYHFRARPWTIAMLDEIDLNKSYNIFRDRFGEAGDFTFFLIGNVELDSAKQMAM
                     RYLANLPTNNREENWRDVGIRPPNGVVQKAVYKGLEKQSYVQITFSGNFDWSRQNRFE
                     LATVTDVLRIKLREQIREEKGGTYGVYVGSSSKQYPYPEFRLTIQFGCDPERVTELTS
                     VIFQEIENLKSEPLGDEDLAKIRQIDLRSYETNLKENRFWLDNLHFYYFNNENPMAIL
                     HYPQLVEDLTKEMVQNAARRYLPLDNYVEVVLYPEQ"
     gene            complement(9291..10226)
                     /gene="trxB"
                     /locus_tag="PKX09_00045"
     CDS             complement(9291..10226)
                     /gene="trxB"
                     /locus_tag="PKX09_00045"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOU16258.1"
                     /translation="MAKDVVYDVVIIGGGPAGLTAAIYTAREMLSTTLLEKSMTGGIP
                     AIVDRIENYPGFPDGISGMELMQNIRAQADRFGANISEFEDVSKIIPGRQCFEIISDN
                     RRFLAKAVIIATGGVPRNLGIASEQEFLGRGVSYCATCDGPLYRNAELIVVGGGNAAI
                     QEALYLTKFAKRVTVVHRRDKLRAQPVLQERAFKNEKIRFIWDSVVSNIFGAEQVEGV
                     EIRNVKSGEKISLPAEGVFIFIGWVPNTEFVRGLVNLDDDGHIITDAEMHTNVPGIIA
                     VGDVRSKPFRQIANAVGDGAVGALSASKYLEGLHN"
     gene            10357..10722
                     /locus_tag="PKX09_00050"
     CDS             10357..10722
                     /locus_tag="PKX09_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HOU16259.1"
                     /translation="MHSEHEIILIGDRVLIDPDEDQNKTPSGLFLPPGVKEKEKVQGG
                     FIVKTGPGFPIPHTDDIEDEPWEEGEDEPHYLPLQAQEGDYAIFLRKSAIEIELESKR
                     YLIVSHSNILLLIRENYLE"
     gene            complement(10829..11701)
                     /locus_tag="PKX09_00055"
     CDS             complement(10829..11701)
                     /locus_tag="PKX09_00055"
                     /inference="COORDINATES: protein motif:HMM:NF028425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HOU16260.1"
                     /translation="MKRKLIILLIFMIAVLSAEDYFTERYDIPFKGEEELDVNISFGL
                     GTLDLKSHDDPKYLVHSIMTYSQRIYKPIVDYKILGNRGRLKMSTEKYDESNKNDKRN
                     LKSSQVNKELNKWYLEFSQKIPIYIAVDLGLGEGRLDFTDLRVRDLKLHCGLSDVNVI
                     FRDKNQDNMRNMVIETGLGSVNVKGLSYANMERFNVECGLGTTELVFDGSLSQDIRGK
                     IEVGLGSVIIEVPDNVAVEVRSQSSFLSSLNLHGFDRISENLYRSGNWETANKRIYLE
                     IEVGLGSVDINWLD"
     gene            complement(11712..13070)
                     /locus_tag="PKX09_00060"
     CDS             complement(11712..13070)
                     /locus_tag="PKX09_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOU16261.1"
                     /translation="MKPKKLFLILLMIGSVGASPDTFEFDGKQYVGNIVKTFAVGSKG
                     DLVMERVSGDVTIEGEARRDVMIVEKFKIDAYSEVNARQILNEEKARYQQRGNKVIVT
                     GKEHSRRYSSDFIVKVPLTFNTTVQTSGGDININKVSGLQNLNTSGGDIEISECSGEL
                     KSHTSGGDIKIQQVAGNLSVNTSGGDIEIELFDGELWANTSGGDINLDKIQGNGQVST
                     SGGDINIVNLRAKTFSAKTSGGDIGADKVEAELTLHTSGGDIIIGQIEGNLEIHTSGG
                     DIEVRQVNKNLKATTSGGNIDIGLIKGYCYARTSGGNIEVDRAYDLEAITSGGDILAK
                     GVVGYIYARTSGGDIEARKLFNSDVKNNAIDLETSGGEIEVILPANLNADIDAEITVT
                     SRRYADVNIHSDFPVQKSHEIKSSRIYIYARGKINQGGSLVRLRTINGNINILKADRE
                     SR"
     gene            complement(13083..14114)
                     /locus_tag="PKX09_00065"
     CDS             complement(13083..14114)
                     /locus_tag="PKX09_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16262.1"
                     /translation="MKHDKHFKELSILYFYNELSESERTDFKHHLDECQVCQAEIERL
                     QIIAKKIDYANEPVPSARRLEALNKQVISKISSESRRPKLVNVKTYFEEMFETITIGL
                     SRPRYQLIAASLILVIGIFIGRVGFRADLPKQPEILANFVNYNYKFTDPEKENIQKAF
                     TNYLLKSGGIEDADLLQSEYRTSQNGLVEVNVRVKEDFAIRGGLDDPTIQNMLMYAAR
                     HNQDAEKRLLALRLLDQIEPNPIVDATFVAAMLHDNNESVRLFAAQLLEKRQMTKQIQ
                     EAFQLVALRDSSASLRKIALENLVNKAEQEVVPVLALVAARDESEEIRQMALLALDKF
                     YDKNDREKR"
     gene            complement(14129..14683)
                     /locus_tag="PKX09_00070"
     CDS             complement(14129..14683)
                     /locus_tag="PKX09_00070"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOU16263.1"
                     /translation="MENEEVELIQNIQSGDKMAFARLMEQYGERVMSVAFRFTRNYQD
                     AEDLYQETFIKVYQNLGKFRFESEFFTWIYRILANQAFNLYNKRKRMTLADPGEDDYL
                     WETIPADAQDEADRVTFSNSIQDQIDAALQQLSAKQRVVFILKHFEGKKIKDIAILLD
                     CTEGTVKRYLFRATHKLQTLLTES"
     gene            complement(14736..16445)
                     /locus_tag="PKX09_00075"
     CDS             complement(14736..16445)
                     /locus_tag="PKX09_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOU16264.1"
                     /translation="MLLILPGIVRAGQPVTIDKFTNQPTIRAWIYLANEPAYNEYFTQ
                     LEYPEKTQRRLAKMGTLAKKTASDLDPGEELRAIIGSNVICLRHYSRILRAFSAEINQ
                     SNFEILTSLPIVTKIELVESFTAKPPDLLKEIPESRTKPLRKMSAAYETQLEQLNIPA
                     AQALGLTGKGVRIGFIDTGFYKDHEVFREIISSGRLIAEYDFINDDDNVSDEKAADTA
                     RNSRQSEHGTAVWSLVGGYNPPDYLGAAYGAEFLLAKTERIDTEIHREEDDFVAAVEW
                     CDYWGADIVSVSLGYRDGFADGSKYPYEYLDGQTAVTTKAVNWAFERGILCVCCAGND
                     ANNFEDGGLLTPGDAFGALTVGAVDAQGEIATFSSHGPTYDGRIKPDLCAMGVATYYA
                     GNSQGAYRTGSGTSLATPLIAGAAALLLESQPELTPAEIITELKRYADRTQNPDNRYG
                     WGIPDVYQSIVNYQPKSFPKGELSFDEIWVYPNPANSNINFYFIWHKATPATAPAYLQ
                     VINLNGQIVFEKRLVSKFGGQKEVINWNLHNQSGARVGSGIYFVCLRSSEYFKINKFI
                     IVH"
     gene            complement(16579..17139)
                     /locus_tag="PKX09_00080"
     CDS             complement(16579..17139)
                     /locus_tag="PKX09_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16265.1"
                     /translation="MNKAICPQIPEGELNFNIILLDNGANVKTWRETVRSVIKDGAER
                     FLLVGRGVPTRRSEKSAGIVVVDQINVSGQNPLVGPNDEEFGTRFPDMTDLYSPKLTE
                     DVLIAGKQAGLVLQKGILLVPVSMTSSTSLEEKLIVQNEIAALSQDVFAGAITVRHAR
                     RQCAGLILFSDIDGKQLGLLLAHLDW"
     gene            complement(17179..17625)
                     /locus_tag="PKX09_00085"
     CDS             complement(17179..17625)
                     /locus_tag="PKX09_00085"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOU16266.1"
                     /translation="MIKTNEIRDRQFRKVFRGYDPVEVKYFTEMLADEFAQMEEKLEK
                     LEAIVNEFDQTRKGRDKILQEAQDRAASIILDAESIASEVIANAETKKRQIQEAIDQL
                     NLEQNKLIQRLRSVLKNQAEILTWLEEAPDWSEEDNTLNESSEETT"
     gene            complement(17622..18329)
                     /locus_tag="PKX09_00090"
     CDS             complement(17622..18329)
                     /locus_tag="PKX09_00090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOU16267.1"
                     /translation="MDSQTIQNNLLIIKERIAQAAQKTGRRAEDIQIIAVTKTFPATL
                     VRQAYTAGLRIFGENRVQEAIPKIIALQDCPEISWHMIGHLQSNKVRKAIENFAMIQS
                     VDSLDLCNKIANISRILGRKVDILLEVNVSGEATKSGLPPEKVFSVVEQVAEMPELIL
                     HGLMTIGPLTNDRDWTRRAFANLKGIFDKIAESRINTGQFNLISMGMTDDYEIAIEEG
                     SNMIRLGRAIFGERRIS"
     gene            complement(18354..20903)
                     /gene="gyrA"
                     /locus_tag="PKX09_00095"
     CDS             complement(18354..20903)
                     /gene="gyrA"
                     /locus_tag="PKX09_00095"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOU16268.1"
                     /translation="MASQVNIEFQRDKIVPVNIDEEMRSSYLDYSMSVIVARALPDVR
                     DGLKPVHRRILYGMSELGLTYNRPTKKCARIVGEVLGKYHPHGDMAVYDALVRMAQPF
                     SMRYPLVDGQGNFGSLDGDSAAAMRYTEARMSRIASELLRDIDKETVKFVPNFDESLE
                     EPSVLPTAVPVILVNGSSGIAVGMATNIPPHNLTEVCDAAIYVIDHPEATVDELMQFI
                     PGPDFPTGAFILGKSEIKRAYETGKGVIKMRAQAFVENSKTGKESIIITEIPYQVNKS
                     NLIEKIADLVREKVIDGITDIRDESDKEGIRIVIELRRDAYAEVILNQLYNHTQLQES
                     FGINMLALVNGVPRQLSLREILQHFIDFRHEIIIKRTQFDLKQAEDKAHILKGLKIAL
                     DNIDEVIAIIKASKSPEDAKQNLMIRFRFSERQAQAILDMRLQRLTGLEVTKVIEEYK
                     ETLKLIERLKFILSNVSEQMLIIKNELAEVKEKYGDTRRTQIITDEGEFSVEDMIAEE
                     DMVITITHNGFIKRFPVDASRRQNRGGRGSLGAQAKDDDFVRNLFIANTHDYLLVFTN
                     KGRCYWLKVHEIPRAGKGSRGRAIVNLINTQPGEEVRAVLGVKEFDDQHFVLMVTRNG
                     IVKKTVLSEFGNPRKPGIRAINLNDNDDLLDVQITNGNQDIILGTAYGKSIRFHESDV
                     RPMGRTAAGVRGVRLSGKDDRVVGVVIVRNESGTILAVAENGYGKRSDIKEYPVIRRG
                     GKGVITLKTSVRNGRMVALREVVDTDDLMIITAKGVLIRLPVATLRTISRNTQGVRLI
                     KLDEGDTISAVTRVVESEDKNNGNGELEESDEITLLENDASDEVAETDEPE"
     gene            complement(20907..>21953)
                     /locus_tag="PKX09_00100"
     CDS             complement(20907..>21953)
                     /locus_tag="PKX09_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOU16269.1"
                     /translation="TRTLNNYAQKNNLFKKESFQLSGDDVREGLTAVISVKVPEPQFE
                     GQTKTKLGNSEVKGIVDSIVSESLAEYLERNPTQGRRIIEKSLNAARSREAARKAREL
                     TRRKSALDIGNLPGKLADCSTRDPSMCELFLVEGDSAGGSAKQGRDRRYQAILPLRGK
                     IINVEKTRIDRVLSNEEIRTIITALGTGFGDDEFDIQKLRYNKVIIMTDADVDGSHIR
                     TLLLTFFFRYLPKLIEDGHIYIAQPPLYKVVCKKQEYYAYDDEELEVLTKRFSKDSTA
                     KIDIQRYKGLGEMNPEQLWNTTMNPETRTLLKITIEEAAAADRTFSILMGGDVEPRRQ
                     FIQKNAKYVKNLDI"
     assembly_gap    21954..22053
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22054..22993)
                     /locus_tag="PKX09_00105"
     CDS             complement(<22054..22993)
                     /locus_tag="PKX09_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU16270.1"
                     /translation="MSEIKFTDAAIDYGAEKITVLKGLDAVRKRPAMYIGDTGKRGLH
                     HLVFEVIDNSIDEALAGFCNKIVVTVHEDNSVSVLDNGRGIPIDIHKEEKKSALEVVM
                     TILHAGGKFDKGSYKVSGGLHGVGVSVVNALSEWMRVEVYRDGKIYTQSYSRGVPTSK
                     VEPVGKTNKNGTLVRFKADVEVFKKIEYEWEVLEHRCRELAFLNRDLEIVLVDERDKN
                     ENRFHYKGGLAEFVNFLDETRPAIIDKPITAEKEEDGVFVELAMQYNNSYNENIFTFV
                     NNINTIEGGTHLVGFKTALTRTLNNYAQKNNLFKKES"
     gene            complement(22990..23265)
                     /locus_tag="PKX09_00110"
     CDS             complement(22990..23265)
                     /locus_tag="PKX09_00110"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOU16271.1"
                     /translation="MESLGTAIARMLKIYGIEKPVRQNEVFFIWQDIVGEVIAKHAIP
                     EKVAYNKLFVKVDSPVWRNELNFRKEEILKRINSQIKNAQIKEIILR"
     gene            complement(23268..24383)
                     /gene="recF"
                     /locus_tag="PKX09_00115"
     CDS             complement(23268..24383)
                     /gene="recF"
                     /locus_tag="PKX09_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOU16272.1"
                     /translation="MVLKNLRLVNFRKYTDSVFVFHEKTNFINGDNGSGKTSLLEAIH
                     YLSLTKSFRTNNDSEAVNYQSDYFNIFGEFLSDNNQTILSNMNYSKTEGRSFLMNKSR
                     VKRYTDVVGCAPVVILSPGLQKITEGGPAERRNFINRIISQTDRNYLVTLIEYRRRIS
                     QRNVVLNQYREKKRCRYDAYFEAQDEILARLGQKLFIGRQAFIADMDSIFKTVFARIT
                     HIGGTVGIDLKYNIKADPDNFISLFTKNLRERFSRDLLIGRTTCGPHLDEIIINYNGR
                     DIRQIGSQGEHKLILIALKLAEGAYIESHQNEKIIFLLDDLFAFLDGKHCRKIVEEIG
                     DKNQALITSTDVKMLRKYGFLDNPEKYKIFNLTTGEA"
     gene            complement(24419..25534)
                     /gene="dnaN"
                     /locus_tag="PKX09_00120"
     CDS             complement(24419..25534)
                     /gene="dnaN"
                     /locus_tag="PKX09_00120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOU16273.1"
                     /translation="MQFSVSQNLLLNNLNKVAKVTPARTTMPILNSILFILKDSNLTL
                     RASDIEITLSTTFPVNGQEDGSVAVPSRLIQEIVGEIREGELTFNATDEGEIKITAGK
                     GKYKIMGRPGEEFPALPSIINQNSILVNSKILSRIIQKTVFAVSRDELKPSLTGVLFE
                     VKADELRSVATDGHRLVYINRKDFSSPDYERQIIVPVKFLNLINTYLTDDNKVSLTIS
                     DNHIMMESDSTLIISRIIDERFPDYESVIPKDNDKELKADSDYLSAVLKRVSIFSNKS
                     THQISLSLNRELSKISTIDLETMSSADEILDVEYSGDDLTIGFNAEYLREIVRNIDTQ
                     KVIMKLKTPVSASLVIPENQEENEDLIMLLMPIRLSD"
     gene            complement(25855..27114)
                     /locus_tag="PKX09_00125"
     CDS             complement(25855..27114)
                     /locus_tag="PKX09_00125"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU16274.1"
                     /translation="MKIKLFFGIIFLATTLFAQTLIDGIAAIVGENIILISEVDQVAR
                     ITASQMNIDPNRDVANFNKIKRHVLNSLIDENVILEQARIETVEVKERDVENAINQRL
                     DMLIEQVGSVENLELLMGSPISKIKKDYRPIFKNQLIVEKMRSEKFSKVTVTRPEVEA
                     FYQTYKDSIPPIPPSLDFSAILFKVTPGPEEEMAAKKLIDSLYTLLKNGYDFEYLAKN
                     YSEDEASAPYGGDLGYIKRGAFIKSFEEVAFSLSPGEISDVIKTDFGYHIIQMVDRKG
                     ESIRVRHILIKIKVSDKNYEDRYRFADSIRTMIINNEISFDSAVVKYSDEPNAKSTLG
                     RIRRIPLNQIQNTDFINVLSNMKVGDISPVFESSPGYYILKLNNVYDDSWPTIERFAL
                     EFKKNQLYQEWLNKLKEDIFIDIKVNY"
     gene            complement(27111..27914)
                     /locus_tag="PKX09_00130"
     CDS             complement(27111..27914)
                     /locus_tag="PKX09_00130"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOU16275.1"
                     /translation="MIFIFLWAACGNHKEDYIARVGNAYLTNEDLTRMLPDGGLSKDI
                     DKNYLNSVVSNWINKEILYQKARQYHFDREEGVQARVADYYRDLTIDSYVRFFLQTNV
                     TISEDEIRDYYLKNKKSFIRDREEAKIVHVLVQDFNDALAIKSAITSRNKNALDTFYS
                     KYKFETSVVKRGESLEEIDRNIFETSPRSIIGPIASNYGFHIVEVLARYPIGSTRTID
                     EVRDEIYQILTQKKIQDQYNALVDSLVRNANYEIKDENISNFLNSIGSR"
     gene            complement(28082..31315)
                     /gene="mfd"
                     /locus_tag="PKX09_00135"
     CDS             complement(28082..31315)
                     /gene="mfd"
                     /locus_tag="PKX09_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOU16276.1"
                     /translation="MKHIDDRIENLVSTFSESSAFLNAVKAFYRSNAGFTVAGLSGFA
                     TSLFLYSFYKKIDQPILLITNDSNEAEYIRDDLELFNLDDSVCYLPEPPNSSFLFSDT
                     ESLNAYFTNDTLQKLSTGQNTIIVATLSSLKSKFPLSDDFNKLKRTYHVNEIVNRDAF
                     IEYLVHLGYTIAPAVESPFEYCIKGGIIDFYPPESMHPYRIEFFGDKIESIRTFNPDT
                     QLSICNLSSFTVQAPAHSSLDNQNSTNIFSYLKKDTVVFCLHAEKLMDTDEKNTTDIF
                     TYLSSYRVINHYDLLASDIDFRVNPQPQTNSIKAFREHLLTVLSAQPDARISLFCANS
                     MQVKRLKELLDLAILNYFDFSISAGAELPEAKLYFYTDHQIFKRERRINFFKKYSPDF
                     PVEQFSPHEISPGDLMVHINYGVGRFAGLDKIAAFGSIRECLCLEYSGGDKVFVPLEK
                     LNYIQKYTGNLQNPPPLNKLGTTEWERTKLKTQRAIDEISKDLVELYAKRISAPGYAF
                     PPDNELQYLMESEFLYEETPDQLTAIQAVKEDMEKPRPMDRLLCGDVGFGKTEVAIRA
                     SFKAVSASKQVAVLVPTTILADQHFAVFSQRLQKYPIKIALLSRFVSHKMQSQIIRDL
                     AAGKIDIVIGTHRLLSKDVSFKDLGLLVIDEEHRFGVKDKERIKFLRESVDILALSAT
                     PIPRSLNFSLIGVRDFSVINTPPISRLPVITEVITFDMNIIKKAILREINRGGQVFFV
                     HNDIKSIAALSHKLALALPEISVDYVHGQMNERELEKTMSAFINNEINLLVTTTIIES
                     GIDIPNANTIFINQAHHFGLAQLYQLRGRVGRSSRRAYAYLIVPEPSRIKPDALRKLQ
                     TIKKHTALGSGYSVALQDLEIRGAGNLLGTSQSGNNIAAIGYELYMKFLREALDENKS
                     TQPAYPEKLKLETEVFCPLPAYFPDSYISDSSARLEYYRQLTLADELSTVDLISGQIR
                     DIYGKLPSEATTLFNISKIRLLASSLGIKKVNIAEKSTSFIWDESFIPPAETDLIQAI
                     QKAASDLGLSYKFNPYNTLYLTVYLKSNFDFDKITRFLNLLRNTLNL"
     gene            complement(31312..33192)
                     /gene="mnmG"
                     /locus_tag="PKX09_00140"
     CDS             complement(31312..33192)
                     /gene="mnmG"
                     /locus_tag="PKX09_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOU16277.1"
                     /translation="MKHLDFDITVVGGGHAGIEAALAAARLGLSTLLVTQNISTIGRM
                     SCNPSIGGLAKGHLVAEIDALGGEMAKLIDRTGIQFKMLNKSKGRAVWSPRAQADKIH
                     YSLLARNTLSNQDRLTLLEDTANSLIVDKQTIKGIHLIHNGDIFCQAVILTCGTFLNG
                     LIHVGLQQFHGGRIDEEPVSGLTEDLARYNILSGRLKTGTPPRVSAESIDFSRTMPQY
                     GDQDPIPFSFSTQNFSPPNIPCFLTRTNTVTHNFILNNLDRSPLYSGIIKGIGPRYCP
                     SIEDKCVRFKERTSHQIFLEPEWKNSNEYYVNGFSTSLPLDVQRKALRTVPGLEKAEF
                     IKPGYAVEYDFFPSYQIKDTLETKSLKGLYIAGQINGTSGYEEAAALGLMASINAALS
                     IQGREPFVLQRHEAYIGVLIDDLITKSPNEPYRMFTSSAEHRLMLRFDNADRRLARKG
                     YELGLLPRETYESTQHKIEVIEEIISFLSSTNISPEAAKVILPNKEAHSIGCNLTLYK
                     LLCRPDVALASLVPFLSSEIKNKIAHFPKIIEEIECDVKYAGYINHSQALVESMSKNE
                     NTKIPPDFDYSAVRSLTNEAREKLIRFRPQTLGQASRIPGVSPADVAALFFLLHKHSG
                     LT"
     gene            complement(33202..34554)
                     /gene="mnmE"
                     /locus_tag="PKX09_00145"
     CDS             complement(33202..34554)
                     /gene="mnmE"
                     /locus_tag="PKX09_00145"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOU16278.1"
                     /translation="MSTENTIVALSTPPGRGALAVVRLSGPKSPDIVHKLTKTTTQLK
                     PRVAQHLNLYKNNELLDDTVIIYYKAPMSYTGEDLVEISCHGSPFIVNQLIELCLRYG
                     ARLAQPGEFTRRAFLNGKFDLTQAEAVADLVFSQTQASHQAAIKSLEGTIGKEFAELR
                     SKIIELITILELELDFSDHEIDTIPPHELSKSIDNILKINSKLLKSYSCGKILKQGAL
                     VPIIGPPNSGKSSLLNAFLNEERAIVTPYPGTTRDSLEEAIQAGGYMIRLLDTAGIRK
                     TKNPIEKIGIDRTKKNIKTGDIILFVIDATQPIKREWLEYYNSKNTIVVINKIDIAPQ
                     KSLTKYEKHFSQYHHIFISAKKRQGIHKLTEMIVDSISQQISNADSLIITNQRHATGL
                     RTAKKYLFSAQKHIRQSLAPEMIIADLRMALNEYDKILGKTSNDEILNNIFQHFCIGK
                     "
     gene            complement(34574..36334)
                     /gene="yidC"
                     /locus_tag="PKX09_00150"
     CDS             complement(34574..36334)
                     /gene="yidC"
                     /locus_tag="PKX09_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOU16279.1"
                     /translation="MDRKSLIGFVLIFVIILTMPYYFKIISKNSPEVTQEKVIEKTPE
                     GVISETIPAKLSESHPSSEVQRPASLGAEKPDTLTIENDLYRLKISSRGGGTITSVVL
                     KNYNKIIGRDTSLVEMIPPFEARRLFIKYIKSNGDSVTIDQNFRIFTEHQETSKNYYK
                     ISDGDSIIIQFQLPAGDDIIVSRTFVFYGDKYIINSYNDLEGILDEMASDKYDLVWEG
                     GIAYTESMIKEDVSYSRASACGGGETENLDVKPGKTGSASLVGATYWTAIKTKYFVAA
                     LIPHQPAEGYRLTGLGVPLQGKDFQKIFNMYLTLNASSENAVKIYLGPLDYSIIKGLA
                     PNFEHLMNFGFSLIRPISKGILWLFTKMHLFIPNYGWVLIIFSVLLKIVLTPLTNSST
                     RSMKEMQNIQPQLNALQEKYASDPQRLNNERLKLMREHGVNPMGGCLPLLLQMPILWA
                     LFIVFRTTIELRQAPFIFWITDLSAPDTIFTLPFSIPIYGSQVNVLPFIMAISQLVQQ
                     KISGASGGKQQKVMMYFMTIFFFFLFNQFPSGLNLYYTLYNILSIIQQKYFPPKPKPK
                     KSRKTNSQRLRQYQTRMRGR"
     gene            complement(36337..36567)
                     /gene="yidD"
                     /locus_tag="PKX09_00155"
     CDS             complement(36337..36567)
                     /gene="yidD"
                     /locus_tag="PKX09_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOU16280.1"
                     /translation="MKIFNTLIILIIKTYRLLLSPLFPNTCRFTPTCSQYALEAFKKF
                     NFFKASWLSLTRILKCHPYHTGGYDPLPTPKE"
     gene            complement(36573..36947)
                     /locus_tag="PKX09_00160"
     CDS             complement(36573..36947)
                     /locus_tag="PKX09_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOU16281.1"
                     /translation="MAEKSSPEDELKDVRSYRPNLPRRERLAKWDQRAQIIHNGKPLK
                     YDCLYISILPQDDWKLSIVIKKECGNAVQRNLYKRKIREAFRLCKPYCKRPAAIAVTV
                     FKKPDNLQVNTLSEILINNLNL"
     gene            complement(36889..37023)
                     /gene="rpmH"
                     /locus_tag="PKX09_00165"
     CDS             complement(36889..37023)
                     /gene="rpmH"
                     /locus_tag="PKX09_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017471599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOU16282.1"
                     /translation="MKRTYQPSNRKRKNDHGFRKRMSTNSGRKVIARRRAKGRKVLSA
                     "
     gene            complement(37212..37760)
                     /locus_tag="PKX09_00170"
     CDS             complement(37212..37760)
                     /locus_tag="PKX09_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOU16283.1"
                     /translation="MDNEYKIYEHNPPHLFCPGSKYIITGATYGKKPWLKSDEAKERL
                     LTSIKKGFDHYHWTLEDWVILDNHYHLMGNAPEDASTLSLMINDIHRFIALWIKKNVP
                     ESKDAKIVMYNYWDTCITYEKSYLARLNYIWYNPMKHGYVEDASDWRFGSYYERIKAE
                     KEEMEELRKKYKWDKVKVKDDF"
     gene            complement(37858..39615)
                     /locus_tag="PKX09_00175"
     CDS             complement(37858..39615)
                     /locus_tag="PKX09_00175"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU16284.1"
                     /translation="MKKIVLIVLFCFILFPVIISAGTWQWTGNLNQARTEHRAILLAD
                     GKVLVVGGIGPGSPGLLSSSEIYDLETETWSLTDSLNIARYDFDLVKLLNNKTLVIGG
                     NVPNVGCVPDCELFDLEDETWSFTGPLNIARNWDHPNHATVLLPEGEVLVINGYTIGG
                     VTTKTCEIYNPETEVWRLTNPSQQGHPDLLGGMLSNGLVLSFHSYPSGSQGPSAEIYD
                     QETEEWTYTGKSIIERNCAVGTIIPSGFLLVGGAWKGQYFKSAEIYDQETGQWSLTDS
                     LKTDGGRLRATVTLLPNNKVLIIGGCKERAIEITPSELYDLKTKKFQEEAPMLYEGPR
                     GGRINHQATLLQDGRVLVTGGKWSGCYLETCQLYSWNSVPTAQVSGSNSGYAGDTLTF
                     SIQVSDAETDSVSVRVSWGDGDTTDWSNFQPNGSEFVFSHTWDEPGEYVIKVQTRDIW
                     TMWTPPSFPSSLQEVTHIHNSLSNWQNLSRVAINPVGIENEQIPLTFNLFQNYPNPFN
                     SSTCISYQIPQDCQVRLSIFNLLGQEVDVLVNEFQRANEYVVNWQPKNLCSGIYIYQL
                     SIESKELKRTWNKKLVIME"
     gene            complement(39718..41451)
                     /locus_tag="PKX09_00180"
     CDS             complement(39718..41451)
                     /locus_tag="PKX09_00180"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU16285.1"
                     /translation="MKTLKISVIIVFFLFDSLLMADEWTIVGQLNLGRRENFQSVLLP
                     DSQSVLITGGLSLGSTEPTSSCEIFDFETGQAVFTDSLPVPLYQHYLIELDLAGDKEY
                     LTIGGMASGPRPPTRRCFRYSSTTNTWQETGQLNSPHYSHAAIAFQDTDSTTKVLVVG
                     GFSAGGSRKICEIYDPQTEIWELTNELNEGRTEHTLVYFPSHQKILVVGDDNSNTAEL
                     FDIPSRTWTLTTPIPVPCPQYENGASQLIIENGQEKGLIMAGTSGGNHWLTTCLIFDP
                     IAETWIETDSLSRGGVAMPSAKLPNGDIIVSGGYRTDTTLLSPVTEIYNVEQGEWQRV
                     ADMNYPRSHHNLVTLADGRVAAIGSFGTTGSYTVELYSWNDSPEIQLIQGQNTGLVGD
                     TLEFVIQVSDPNQDSVSIRIDWADGDTTDWQEYDVSGTEFCFSKVFAEAGEYKIKVQA
                     KDIWSEQGIHNSLSNWQKLLRVVINPVGVGIDEKIPLTFNLFQNYPNPFNSSTCISYQ
                     IPQDCQVRLSIFNLLGQEVDVLVNEFQRANEYVVNWQPKNLPSGIYIYQLSIESENFK
                     RTLKKKLIMME"
     gene            41977..43368
                     /gene="dnaA"
                     /locus_tag="PKX09_00185"
     CDS             41977..43368
                     /gene="dnaA"
                     /locus_tag="PKX09_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOU16286.1"
                     /translation="MPNSLICSDVWNSCLEYVRKKIPQQTFETWFAPMKAISLADDTL
                     ILQVPNKFFYDWVDNHYREMILKALKEVTGNGFNVSYTILLGEELEQPAGTLSVSKRG
                     SGGTPVAEPDYKAGAREQGTRLNERYVFDTFVEGASNQFARAAAMAVASAPGRTSFNP
                     LVIYGGTGLGKTHLLQAIGNEALNNGRAKKVVYVSSDTFTLDFIAAIQNNKTTEFSRS
                     YRSIDMLLLDDIQFFQNKEQTQEQFFHIFNDLYQRHKQIVLTTDKPPMELRGLQERLL
                     SRFQSSLTVDIQPPDLETRIAILQKKANDDGLDIPYEVIEYLSANIRSNIRELEGALI
                     RLLAYSSLLQVDIDINLTQRVLREILGTRHSSVLSIESVQSTVSTVYKINLDSMVGKG
                     RTKEVAEARMIAMYLTREFTDLSLKTIGLYFGGRDHSTVVHACRWVEERLQSDSVFAR
                     QINDLKAKLSQNI"
     gene            complement(43375..44919)
                     /locus_tag="PKX09_00190"
     CDS             complement(43375..44919)
                     /locus_tag="PKX09_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOU16287.1"
                     /translation="MTKFSLVKSAAVLGIDAYIVDVECNLSNAQLPAFITVGLPEGAV
                     RESKERVWSAIKNLGYYVPSNKIVVNLAPADIKKEGSSFDLPIAIGILTAADLIERHK
                     VSDYIFLGELSLDGMLREIKGALPISVSVRNSGIKGIVLPEQNAREAAITGDLEIIGL
                     KSLQEVIDFLENRTHPEPTHVDVRALFEEHRQYQIDFSEVKGQENVKRALEVAAAGGH
                     NIIMIGPPGSGKTMLAKRIPTILPNLTLEEALETTKIHSVAGLVPSGQGIVATRPFRS
                     PHHTISDAALVGGGKFPRPGEVSLSHHGVLFLDELPEFKKNVLEVMRQPLENGYVTIS
                     RALLSLTYPASFMLVAAMNPCPCGYATDPHHQCTCTPQQIQKYMARISGPLLDRIDIH
                     IEVPAVKFSDLSAKAPGEPSEIIRERVQAARLKQTARFAGIKGVFANAHMESRLIRRF
                     CQIDQTCEDLLRNAINKLGLSARAYDRILKVSRTIADLSNSEKILPEHISEAIQYRSL
                     DRHLWL"
     gene            complement(44986..46086)
                     /gene="plsX"
                     /locus_tag="PKX09_00195"
     CDS             complement(44986..46086)
                     /gene="plsX"
                     /locus_tag="PKX09_00195"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOU16288.1"
                     /translation="MNNLDNKQPSAVIAVDAMGGDFAPDEVVLGAARASVETPIKIIL
                     VGDQSKIEPILEQTEHNPQQLEIVHTDQYITNDDKPKFILSKKPRASMLIASQLCGAD
                     QAHGIVSAGNTGAYILSAIKYIPRIEGVHKTAIASAYPTRNEQGRSDPFALLLDVGAN
                     VHCTADDLVQFAIMGKIYASAVKGIENPTVGLLNIGKEPYKGGDTLTDAFNMLNSIQD
                     MKFIGNIEGNEILKGIADIIVAEGYVGNIVMKTIEGFGETISHIGRYAFKHRFIWMLG
                     LLALSSGIKQLKKVADYSEYGGAPLLGFRKIVIKAHGRSRAKAITNAIIVAAKTYHDN
                     VCEKMAREIAQYEKQFQRKNVMNCDWKLNFDQ"
     gene            complement(46083..47048)
                     /locus_tag="PKX09_00200"
     CDS             complement(46083..47048)
                     /locus_tag="PKX09_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOU16289.1"
                     /translation="MKIFTYIEPENKNISKNSAGCLTLGRQLADLANGSLTAIVPAQE
                     NSTMQNECGRYGADRVAFTKNFDKYIPEVTASGVANILLQENGDILILPDRPAIRDIA
                     AIACETVSGSIAVDCLQVNLDSENSPIVRRAMYGGKALADLKLIKKPVFITIRANIIP
                     PQENIRSAEIINSPSQLSAVTPKIKINEIRPVASKRPALTDAKIIVAGGRGIGAPENF
                     HLIEELADALGAAVGASRSVVDAGWVPQTFQVGQTGKTVSPDLYIACGISGAMQHIAG
                     ISSAKYIVAINNDPDAPIFKIANYGIVANLFAIIPEMIKHLKNIK"
     gene            complement(47061..47813)
                     /locus_tag="PKX09_00205"
     CDS             complement(47061..47813)
                     /locus_tag="PKX09_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOU16290.1"
                     /translation="MKIVVCVKVVPVSDSIITITPEKTAIDLVNIKFDLNPYDEFAIE
                     AAVLLKEKNGGEVIAISLGPERDNISLKRVLALGADRMIHLVTADKALSDPLATAKAL
                     ATEIKPLSPDLIFCGKLSIDTGNGLVGPLVACLLEYPFLTGICELQVGTDRLTAAKDT
                     EEGRIIYESSLPCLLTTEKGLNKPRYPSLKDIMLSRKKEIITKNVNLTEKASDIIQLS
                     EPAPRPAGTILGQNVDAVPELIRRLREEAKVL"
     gene            complement(47825..49171)
                     /locus_tag="PKX09_00210"
     CDS             complement(47825..49171)
                     /locus_tag="PKX09_00210"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOU16291.1"
                     /translation="MQELVKIAENVISEARRGGADEVDVLVNNFTNTRLKIRLGKVEE
                     LRQSNPKALGIRVFKNKRKALTYTSDLRPESISKMVQKALEIVRVSSTDEYNGLPEKH
                     LFGKVQADLKLYDPEIAQIPTERKIKMATELEDLGLKQDPLITNSGGSFWNDSYGQSI
                     LINSQGFSGVQEFSSCSLSLQLVAEKDGVKQTDYWWSGQRAFSQLDSIESIAKTAAAR
                     TIRKIGARKPTTQSVPVVYDPQTGADLLAILAGVVLGSAIYRKNSFLVDKLNQPIAVE
                     ELNIIDDATLPGGIDSRYFDDEGLPARRNIVIENGILKTYLCDCYSARKLKCTPTGSA
                     RRGTSTDPSPSTSNFFLPAGKYSPEEIIGSVSNGLYLTDLNWVGINYVTGDYSRGAEG
                     IWIENGKLAYPVQEFTVAGNVLEMLKSISMIGNDLEFRGSINSPTFKIDRMMVSGQ"
     gene            complement(49186..50706)
                     /locus_tag="PKX09_00215"
     CDS             complement(49186..50706)
                     /locus_tag="PKX09_00215"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOU16292.1"
                     /translation="MKRREFIKKSGVIAAGIVTAPAWFDMAGFSKTVSETQDLENLFN
                     ISAVEAEKLLRLALSRGAQSADLFFEYRPNSSLRIEDGIIKDIQYGISLGMGVRAIKG
                     DQIGYAYTDAIDFQSMKRVAETAANIANSTESFKPAHFQKVAAKNLYPVKTYSIDMDL
                     NPKITMLNEADKIARQFNARVSSVIASFSEELRQIMYFDSNGKKFQDIQPLMSLRVQV
                     IAEDGVRRQGASASRSARAGLEFFEKPENTSAAIARQAAATAITNLDARPAPAGPQVV
                     ILGAGESGVLIHEAVGHGLEADYSYKNLSNYSGKVGQKVAAENCTIIDSGLFPGKRGA
                     LNVDDEGNLPTTTVLIENGILKGYMHNSISARMMKSNPTGNGRRQSYAYPPLARMTTT
                     YLQKGNYDSEEIISSVKKGVYAKGFSGGQVDTIKGDFTFGVTEAYLIEDGKLTAPLKD
                     VTLIGNGPDTMNKILMVGNDLKISDGTWSCGKDGQNIPVSVGNPTVKISEITVGGS"
     gene            51027..51458
                     /locus_tag="PKX09_00220"
     CDS             51027..51458
                     /locus_tag="PKX09_00220"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOU16293.1"
                     /translation="MVKKSVLFICTGNSVRSQMAEALLRQLGGDQFEVFSAGSTPVGI
                     HRFTKQVLAEIGISVDNQYSKHIDMYRDYNFDYVIVLCEVAALNCPTFPGQVERLEWL
                     IDDPIRVIGNEERRLMAFRITRNILKERIEKFIKEKGNPST"
     gene            51614..54730
                     /locus_tag="PKX09_00225"
     CDS             51614..54730
                     /locus_tag="PKX09_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16294.1"
                     /translation="MIKKLKSILLILIFATAGFSQYGKNKVQYDTFNWSYLQSENMDI
                     YFYEGGESLAEFAAPVGEQALKDICKVLNWRLRKRVTLIVYNSHSDFQQTNVTLSYLY
                     EGIGGFTELFKNRAVVPFEGSYFDFWHVIRHELVHVVINDMIYGGNVQSVVSGRVRLS
                     IPDWMSEGLAEYIAYGWTAQTDLIMRDIALNSNIPNIEELDYYSAYQGGNSVYRFIAE
                     KYGIEKIGQIWAQMKSKGNANNGLKAAIGMDMKELTDKWHRWMRTEYWPDVADRYDIE
                     EISMKLTDHKKLKNYFNTAPAISPLGDKIAIMTDRNGYADIYLISAVDGKVIKKLISG
                     QRTPDLEELKWLNPRLSWSPDGKKVVLAAKSGGHDALIIADVETQKRQRFEFKELEEI
                     FTPVWSPKGNSIAFVGLKNGQTDLYLYDIKKKSLTQLINDKYPDFEPSWSPDGQRIVF
                     VSQRTPEIDSLPNLPEIMNVKCFQTDLFCLDLDNGEITRLTDSPWNENYPCWAHTKDA
                     IFYTSDYHGISNIFMLDISTGESGAITNVLTGVFQPSISNDDLRLVLAGYADEGWDIY
                     SISNPLQLWDERQEIKPTVYAKALMESWENPEKYQRFNFPSKEEAKPRTRQNIIAAGD
                     FSNYIFAPMYNYEISAEPEEDFLDSTMASEDTTKQTTKSGRYPVFPYKTNFSLDVAYG
                     EAGYSNLWGLQGTTVFYFSDVLGNHQLALGTEMYIDLENSDYYLSYMYLAKRTNWGIT
                     GFHTSNFWNYGFFTVFRLRNYGLDFSASRPSSHFSRTELGLTSYNVEQKYILTLTGEE
                     LYTYRINTILPRVGFVYDNSLWGYLYPIDGWRARVDFIASPKYSAKSLEFYSVQADIR
                     RYLKLNMDYSFGLRLSTGFSNGKNAQRFFLGGEDNWINYYYRYDIFTADSTLAENIYF
                     SDFITPLRGALYYERAGNRYFLFNLEFRYPFIRQLVLGWPLPVTMGGIQGVTFLDFGS
                     TWYDNKFHPFKNDYYRGFMMDDLVAGYGMGARIYLGVFVLKIDVAWRFDFDRTYSPRW
                     YFCLDTDF"
     gene            54818..55726
                     /locus_tag="PKX09_00230"
     CDS             54818..55726
                     /locus_tag="PKX09_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU16295.1"
                     /translation="MKYFKLILICFIWSTTWAVIKIGLDRTPLMVGLALRFSVATIAL
                     GIIIFLKRRKIAFDRVSVQNYLVVGLLTMALSFYCTYWAEKFIPSGLTSILWTAMPIV
                     VGIFAHFMLPDERLRLAQVMAILIAMAGVIVILSDRQLVFGRELLWGSVVALFGVVVS
                     SWPNVLLKTRPQPYDSLTMTAMAMLIAALVNFIGATVGGEWQKMVWDFKNIGAIVYLG
                     LFGSALAFYIYFDLLKQINVIKLTFINFISPIFATLIGALWLNEIVTWREIGGMLIIF
                     FGLFLYDKDKYLAILKIKNGRSTHAR"
     gene            55716..57068
                     /gene="radA"
                     /locus_tag="PKX09_00235"
     CDS             55716..57068
                     /gene="radA"
                     /locus_tag="PKX09_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOU16296.1"
                     /translation="MPVKVKNVFVCNECGSVQPKWLGKCPECGAWNSMVAEQIQKKPR
                     SSAVERTRRNLVPLNQASTTSTQRLVTGITEFDRVLGGGFVSGSVVLIGGEPGIGKST
                     LIMQVIARLPGDRLLYFSGEESEEQLALRARRLEINDDRILVACENNLENILDQIENL
                     KPTAVVIDSIQTVYSDQFENVPGSVTQVRECAGRLLHLSKELNITTVFVGHITKDGMI
                     AGPKVLEHLVDTVLYLEGNKNNFYRTLRAVKNRFGSTNEVGIFEMKERGLIAVDDPAG
                     FFLTERREYVAGSAVTVSMEGTRPFLIEAQALVTQSSYGTPQRSANGIDYRRFVMLVA
                     VLEKRAGLPLRTQDVFINLAGGFKIDETGIDLGVTVAVASSFKDVPLEPKAAFIGEVG
                     LVGEVRSVPFLENRVREALKFGFTTVYVPKSGIKILEKSLQSKVTGIESLNQLFGFIF
                     "
     gene            57084..58331
                     /gene="tgt"
                     /locus_tag="PKX09_00240"
     CDS             57084..58331
                     /gene="tgt"
                     /locus_tag="PKX09_00240"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOU16297.1"
                     /translation="MSNHGRKESKEKNQLPFDIIAQDNGTKARAGIFVTDHGMVETPV
                     FMPVGTSGVVKTLTPTELQECRVQIILGNTYHLYLRPGLDVIQAAGGLHRFNAWPGPI
                     LTDSGGYQVFSLAHLGKLTEDEIVFRSHIDGSEHLLSPAISMEIQRTLGADIVMAFDE
                     CTPYPCDREYARQALERTHRWERLSREHFLKLKPLYGHRQYQFGIVQGSVYPDLRAES
                     VKALTEVDFDGYAVGGLAVGEPKEQMFEILSLVAPQLPEKKPRYLMGVGKPEDIVRAI
                     GWGIDMFDCVLPSRNARNGTLYTWKGRIVLKQSQYRMDFASPDPNCSCYTCRNFSRAF
                     LRHLFMNGEMTGLRLNTLHNVHFFMELVSRARQAILSGDYENWQTQFFRNYPEEADHS
                     LENEARRSERRRKHLEENDINSV"
     gene            58384..59469
                     /gene="nadA"
                     /locus_tag="PKX09_00245"
     CDS             58384..59469
                     /gene="nadA"
                     /locus_tag="PKX09_00245"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOU16298.1"
                     /translation="MNLNVSYPPPDLARLSQEEIERRIGKIRAQFGPQLIILAHHYQC
                     DQIVAQADAVGDSFALSRRASETKAKFIVFCGVHFMAETADLLKSAEQKVFLPNVDAS
                     CPLAEMANIENVTTAWGKIQALTGDQLIPVTYINSRLELKAFCGKNGGIVCTSSNAQA
                     ALEWAWSKKPKVFFFPDQHLGRNIALKMGISPQEIVIYDPRRPIDDKFAWQIKQARLI
                     IWSGFCEVHQAFSRNDIERLRQAIPGIKIIVHPECPEEVALNADFVGSTEYIIKTVTA
                     GAPGSAWGVGTERNLVDRLARQLSDRKVISLQEKGAYCSTMALVTPIHLLYQLECLRD
                     GRLVNQVVADDNLRTDALLAVQRMMEL"
     gene            59472..61040
                     /gene="nadB"
                     /locus_tag="PKX09_00250"
     CDS             59472..61040
                     /gene="nadB"
                     /locus_tag="PKX09_00250"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOU16299.1"
                     /translation="MKAAEPYRMTDVLVIGGGLAGCSAAYAAAQAGLRVVLLTINQDA
                     SVSSSYYAQGGIIYKNPADTLADYIADFQESGAQLVNEKVVEAIYTNGPRLIEQILIG
                     ELGVPFDRTPQGDIALTREGVHSRPRIAFVKDYTGRAIQERFHKKIRQVENIEVLPNT
                     TCVDLITLAHHSLNPLHIYEPSTCAGAYVYFQDSGEIHPILAKQTILASGGLGRLYLH
                     TSNPYHARGDGYAMAYRAGARLMNMEYVQFHPTTLFSTEDERFLISEAVRGEGGILRN
                     KDGEDFMPRYHPLGSLAPRDVVARSVMQEISRTKAHFVYLDITHKSADWLKQRFPQIY
                     EKCLSVKIDMATEWIPVVPAAHFECGGVAVDLLGNTTIRRLKAVGEVACTGLHGANRL
                     ASSSLLECLVFGTLAGRDCAERIGSVEIITPQVAPWQKENEPLDPDLIHQDRLTIRHT
                     MWNYVGIVRNAKKLDRAGGILWRLQQDIEKFYAHSQLNDELIGLRNAATTTLLVLHAA
                     RLNKFSRGCHYRVD"
     gene            61069..61398
                     /gene="yajC"
                     /locus_tag="PKX09_00255"
     CDS             61069..61398
                     /gene="yajC"
                     /locus_tag="PKX09_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOU16300.1"
                     /translation="MNSLLYAGATGGGSGAQPANPILSLLPFLLIILLMYFLMIRPQA
                     KKQREKAKMLQEIQPGDEIVTIGGIYGKVEGVKDKNILIVRVAKDVKINISRSAVASK
                     VEEKVVS"
     gene            61402..61938
                     /gene="def"
                     /locus_tag="PKX09_00260"
     CDS             61402..61938
                     /gene="def"
                     /locus_tag="PKX09_00260"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOU16301.1"
                     /translation="MAVLPIYIYGSPILHKKLAPIDDLPPDFDRILSDMWETMYDDEG
                     IGLSANQVGLDLRFFVADFSLREENLGREVIINPVIIKVEGEVSSEEGCLSLPNIREQ
                     IPRAEKIILRYENRERKVIEREFNDLVARVIQHETDHLDGLTMVDRISPLKRSFLRSK
                     LKRLATAAAEGKFAKDIE"
     gene            61945..62874
                     /gene="fmt"
                     /locus_tag="PKX09_00265"
     CDS             61945..62874
                     /gene="fmt"
                     /locus_tag="PKX09_00265"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOU16302.1"
                     /translation="MKIIFMGTPEFAVPSLRIIADSRHEIKAVVTKPDMPCGRGCRLQ
                     EPPVKKVARQLNLPILQPDSLKSPDFLAEMAQFSAELYVVVAFRILPPELLVIPPRGA
                     INLHASLLPRYRGAAPINWALINGEKETGVTVFQIKPTVDTGNILLQERIAIQPTDTY
                     GTLAPRLAEIGARLILAAIDGLEAGTLKGTPQDDSLATPAPKIFPELGEIDWYKPASD
                     LKNLIHGLSPTPGAFTFFRGKRLKILRASVIDEKIPGEPGTIIQCNKNSLNVLTGSGV
                     LKLEELQFEGRKPLPAAEFLCGFGGRVGDKFAS"
     gene            62871..64190
                     /gene="rsmB"
                     /locus_tag="PKX09_00270"
     CDS             62871..64190
                     /gene="rsmB"
                     /locus_tag="PKX09_00270"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOU16303.1"
                     /translation="MNTRAAAVMILNTLPATGYNYESAIDAFLKINNCSSPDSDFIYL
                     LVKGTIQNQSLLDYLIEQTRNNSVKRLEKIARNLLRVGVLQIVVLKTPPHAAVNETVA
                     AAHQLQRSDLAALINGVLRHIPQESVWRERLGQLELAEALAIEFSHPRWLVEKWLNAF
                     GRENTIDLLAFNNKYQAIFFRHNPLRIHWSEFEAKLQADNYRIEIYETAPVVFFLVDR
                     PGDLLKSEYFECGYCSVQDYSQSLAVRLLDPQPGEKILDACAAPGGKATMIAQLTKNR
                     ANILANDRTINKIESIKESACRLGIDSLNYSVNDVAAAKFPMFDKILLDAPCSGSGVL
                     ARRADLRWSRKPGDLLQAQALQLRLLENLAGFLRDKGVLVYSTCSIEPEENWETVQMF
                     LERYPHFSVDTAVKYVDQRYCDQKGAVMVLPFEHNLTGSFAVRLVKH"
     gene            64212..64946
                     /locus_tag="PKX09_00275"
     CDS             64212..64946
                     /locus_tag="PKX09_00275"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOU16304.1"
                     /translation="MSTKIRNCLRFIAAFIAVGVVLVLVFDKILMPIYVRHGKEVAMI
                     DVLKLPADKAIQILDANGFEVQVVDTIESGNLPIGIVIDQQPPPGRMVKKGRVVQLVV
                     TGGSRFVRMPNLVGRALKAAQIILENNKLVLDSVEYLYSSEIPEGVVSEQSILPGSMV
                     SINTKVTVVVSKGPPSRQLEVPMLIGLSLTEARASIRKAGFRVGVIRYVPTTDLNPYT
                     VIDQTPKPGGLYDNPINIDLQVTIEP"
     gene            64936..65601
                     /gene="rpe"
                     /locus_tag="PKX09_00280"
     CDS             64936..65601
                     /gene="rpe"
                     /locus_tag="PKX09_00280"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOU16305.1"
                     /translation="MNHNKPTPEVVPSLLSADFARLKEHIRLVEKAGARRLHLDIMDG
                     HFVPNITIGPLVVEAIRRVTNLHLETHLMIERPERFIQQFVEAGSNTVILHVESAQDL
                     HNDLRKIKEFGVKAGVVLNPPTSFEEVEPYLDEIDHLLVMTVHPGFGGQQMIPEALNK
                     VRRAQPWQARYGFLIEVDGGINQETITIAAAAGADLLVAGNAVFGSGRPGHAFRSLTW
                     QIK"
     gene            65598..66206
                     /locus_tag="PKX09_00285"
     CDS             65598..66206
                     /locus_tag="PKX09_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16306.1"
                     /translation="MIKPVNEGELGRKAIHYFSSLIPIIYHFFFTRNEAVVIIGILFI
                     GIVVAELMRMHLPALRQLYYRLFGWMIRPHEFHNHFTGATYVFMGAFLAIFLFPKEVA
                     VTSLLILTVGDPTACLVGLSIGRIKIYNNKTLEGALAFIFVSLIATVWISSIPFWIKL
                     VGAVVACCVEVFHRKIDDNVLIPLISGTTIFFLVELGNFLNI"
     gene            complement(66282..66989)
                     /locus_tag="PKX09_00290"
     CDS             complement(66282..66989)
                     /locus_tag="PKX09_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16307.1"
                     /translation="MKKKILYLIPMICAVFAVSSFAQSASSNTTNQFFEVDKSIEELR
                     QEIAEIRRQIRDIELRTSTPEIRKEINRLIPVPDITHEIILHNGTVVQGKIIHETIDK
                     IVIQTNIGQLTISKNDIHVFREAEKPHAKCVLVGPITPKIFEDKTTYTGKIKNEGERR
                     ADFPQIKIQLFDEATNLIAADSIIIAGNYHMFLSGVQTDATIEPGQTFDFEITISYPA
                     NTKVSYFIPKITWQEFD"
     gene            complement(66986..68080)
                     /locus_tag="PKX09_00295"
     CDS             complement(66986..68080)
                     /locus_tag="PKX09_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16308.1"
                     /translation="MKAKNIIIIMLVLGGLMSCAPKTTTVDLRAVEKLRVDYNRGKIQ
                     KLDDLIAIYQDQAQPIETRIAAVNALAETQHPDAIKAMHDFMDQSVGLNYALLNATAT
                     ALTKHATPENVAAMVDGIVSAQKKYINFRTGVMEKMEDLNVALQVEALLKLYQSEKEN
                     YIFMQESLTKLLGSIPDDRVIPILINIAKDESVNISTRSLAMEILGKKRHPLITETFI
                     EMLGDPEQQLALRGFAIKSIEDVKESRVILALLETLNQSREEHLVLLDALTRAMGDYS
                     DPAVIPALVEIAKNTDYPLATRRNALSALVKFQDVEAFKQLLPIMESPEGYVLYDEMA
                     QIATAIGQPEVLNQLRLAALKAQLKAREAQ"
     gene            complement(68134..68916)
                     /locus_tag="PKX09_00300"
     CDS             complement(68134..68916)
                     /locus_tag="PKX09_00300"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOU16309.1"
                     /translation="MNQNVYFIADIHLHLNDSQEEKVKRERLFAFLAEVRRHQGSLYI
                     LGDMFDFWFEYRYVIPGVFFPVLCELRETVLAGCEVHFLGGNHDYWAGQFFTDVLGIT
                     VHYKPIDTIIAGKSFHLTHADGILHNDRGYRLMRFFFRNKTIIRLFRYLHPDCAIRLA
                     THLSGRSRRLTLRSPAQEESDRQEIIAYGKKKLEAGAEYVITGHLHLPTEYHTERGVV
                     VNLGDWMKYYSFAFFDGKSLRLCYWPLSTSDSTGKKSEFLLD"
     gene            69158..70243
                     /gene="buk"
                     /locus_tag="PKX09_00305"
     CDS             69158..70243
                     /gene="buk"
                     /locus_tag="PKX09_00305"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOU16310.1"
                     /translation="MIETVLVVNPGSTSTKIALFDRSRRLQFENISHVGTPISRIHSI
                     AEQLPLRIEIVTNTIKPWLFDYCLKAVVGRGGLLKPLRAGVYKITPGMITDAISCKYG
                     EHASNLGAPIAEVIANQHHISSFVVDPVTVDEMIPEARISGIPEIERVSRQHTLNIRA
                     CVRKASAQLGRLPSELNFVVAHLGGGVSIAAVRGGRLIDCNNALLGMGPFTPERAGAL
                     PLEGILNLAYSGKYTKEEMKIKMTKKSGLVAYLGTNDAREVWQRAETGDQRAALIFRA
                     MIYQVCKEIGAMATVLKGNVDAIIVTGGQARGAETVEMIHAGVGFIAPLIVFPGENEM
                     EALAEGGWRALDGNEEILIYEEINVKE"
     gene            70247..71152
                     /locus_tag="PKX09_00310"
     CDS             70247..71152
                     /locus_tag="PKX09_00310"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOU16311.1"
                     /translation="MLKTYSEIFEKIKKNARRPTIAVALAEDKSVMEAIKVLSANDIA
                     RVILTGNPKKIKSYAEQVGYPIKEDEVIPAFSEEEGATRAVELVHLGKAEILMKGHIT
                     TPALMKAVLDKEKGLRSGDLLSHITVVAVSNYHKLLMVTDGGVNIAPDLEKKKAIINN
                     AIIFAKKLEITRPKIAVLAPIEKVNPKIPETVDAMTLQEMAERGEFGSAIVEGPVAAD
                     VALSARAAERKGLHSRIAGDTDIILVPGITCGNAIIKFLMLLAKAEVGGVVVGGRVPI
                     VLLSRSDEPAEKLNSTLLALLMSAL"
     gene            71258..72277
                     /locus_tag="PKX09_00315"
     CDS             71258..72277
                     /locus_tag="PKX09_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOU16312.1"
                     /translation="MSFEKLIKPGTEFLGVKYPIICGAMTWVSDYNLVKTVSETGAFG
                     VLAGGNLPPDLFEQEVDRCIAGVKKPFAVNLITIGPNFRIHYEILQSKNVPFVVFAGG
                     IPRRQDIEGMKTRGKRTLSFAPEESIARQQIEYGIDALILEGSEAGGHIGYVSLIVLL
                     QQVLFKVKGLPIFVGGGIADGRMIAHLLLMGAAGVQMGTRFVLTEECRVHPNFKAALL
                     KAKARQAIATPQYDSKLPVVAVRAIKNKGTDEFGKLQMRLLQEIAAGKITREKAQYEV
                     ENFWVGALRRAVVEGDVDYGSLMAGQSVGLINEIVPMQKMINDLIKEARAEIKVIRNR
                     LGQCG"
     gene            complement(72299..73129)
                     /gene="ybgF"
                     /locus_tag="PKX09_00320"
     CDS             complement(72299..73129)
                     /gene="ybgF"
                     /locus_tag="PKX09_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HOU16313.1"
                     /translation="MNFKPIRPIIYGLFVISLTACATQKDLLKIQEDLYFLRNQIQSV
                     KQTTEENKQLLSQVPTIAVQCNQILIELDSLEKSLAELQDANDRTRADINTRLSSLKQ
                     DAAIISSKLDDASYRSSQLIGKVESLTGKMTNISEKIESRAATPNSAEPAPTEIFNNA
                     YRDMTKGNIELAIQGFQTFLKLYPDKELADYCQYYLAEIAYQQEDYATAATEYKLLIN
                     NYPRSQKITNAHYKLGLCYLKLNNLEQARTQFETVIREYPNTEEALLARNQLQKLEQQ
                     "
     gene            complement(73161..73775)
                     /gene="pal"
                     /locus_tag="PKX09_00325"
     CDS             complement(73161..73775)
                     /gene="pal"
                     /locus_tag="PKX09_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HOU16314.1"
                     /translation="MKKLLVFLIPLLCIVLMFNTCSKKTAGVQEDKGETPAKVTPAPE
                     QPKPVQKPDTLAQAPAPVVTPPPAEPKPVIADTQKVAAIAEPSLKDELAQCNTVYFDY
                     DRYDLRPDAIATLEKAAKVLKQYPNAIITIEGHCDERGTVEYNLALGERRAHAVKTYL
                     INYGIKPDNLITISYGKERPIDPGHNEEAWAKNRRAVLVTSDSK"
     gene            complement(73836..75029)
                     /locus_tag="PKX09_00330"
     CDS             complement(73836..75029)
                     /locus_tag="PKX09_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOU16315.1"
                     /translation="MFLKIQSDETRKINVAIAEFGTNQNPELAINLRQIIIDDLNYSG
                     FLNVVDPLISPAGDLIFDNSEVIITGDVTHKSSYFNFVFGLTESVTGRKLMTKSLRSY
                     PFALRVLAHTVSDEIVYQLCGEYGNACTKIAYVTEQKGGKELALMDWDGQRATLLTRN
                     RSINLLPCWSNDGESLIFTTYAFGGGPILARYIFKDQQIERFISRAGTFTSPEYSPNG
                     KKVAFVLTHEGNADIYTADRNGQNLQRLTSNPAIDTAPSWSPNSREIVFTSDRSGSPQ
                     IYIMDAEGGNVRRLTYENNYNSSPKWSPKGDQIVFVSYQSRGFQIYLIDVTGENLICL
                     TDNTGSYENPTWSPNGSHLVCASNRSGRWEIYSMLRDGSLLRQLTFTGGNTGPAWSPR
                     LKPFN"
     gene            complement(75106..75786)
                     /locus_tag="PKX09_00335"
     CDS             complement(75106..75786)
                     /locus_tag="PKX09_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOU16316.1"
                     /translation="MNYTFAISLILHLTFFAVVSVLAGRHQPRVMAIPDQNIRWVNIA
                     PPVVQTAEAPIQVKPATSVPVPKPKVEKAATVLAEKPQKPQETKPLPEETIASSTTSP
                     ELPVEPEATSQPASGGIPGATGLRVDDPDFNFIYYLNIIRNRIQEHWRPPYSSADSPY
                     SQQVMIAFKITRSGKISDVRIEQSSGNFLFDQAALRALYETATLPPLPSEYGGKELNV
                     HIEFETLK"
     gene            complement(75783..76187)
                     /locus_tag="PKX09_00340"
     CDS             complement(75783..76187)
                     /locus_tag="PKX09_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOU16317.1"
                     /translation="MVDRRYKPLTEINITNLVDVTLTVLIIFMITTPLMYSGIEVDLP
                     ATRREITTVQEGILVNYSLDGQIFIDGQPVMENEFEKTLVKRWVQVGKKPVLLSADRK
                     IEYGKIIKLIDRIKACGVTELGLIVETEQDKK"
     gene            complement(76168..76875)
                     /locus_tag="PKX09_00345"
     CDS             complement(76168..76875)
                     /locus_tag="PKX09_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOU16318.1"
                     /translation="MNTIFSSTYDIGIMGWVIILILIGLSIIAWGIIIEKIILFRRTE
                     KQNKKFLAIYQSYSDWSAVARETQHLKSGLLLNLFRKIYAEARKDAELNPGNVVTENI
                     DTDASRISSLELYKALVDSAISTELIPYEKRLIFLSSTVSASPFLGLFGTVWGVMQAF
                     LSIGVKGSAAITAIGPGIAEALITTVVGLAVAIPVLFAYNLIVDKIRKYENQLQILAN
                     DMIQKIIRENFYGGSQI"
     gene            complement(77017..79074)
                     /locus_tag="PKX09_00350"
     CDS             complement(77017..79074)
                     /locus_tag="PKX09_00350"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOU16319.1"
                     /translation="MKSKAVGLLWLSLIIMVEVGFTAKRSMQIDDLLNCHRLAEPQYS
                     PDGQWIAFTVQTIDIEANKGQKTLWRIRPDGQNLQQIPTHSGNSWSPLWSPDSKSLAF
                     LSDSGGQVQVWKLALDNNHVQQITDFYGGVESFCWSHNGQKIAFSAHVYPDLTDYAEM
                     KKRDTARQASPIKARVYEELMFRHWDEWWDYKRSHIFVLDLNNGSLTDVTPGDFDAPP
                     IALGEGYCFSPDDQTIVFNSNHDPVIAISTNNDIWSVSIANPREPRLITTAQEYRDFR
                     GNDYAPRFSPDGKYLSFLSMVRAGFEADKSDAFLYEMATGKFINLTEKNDIRIQAYEW
                     FPDSRGLLLLIEERGRLTLNTLNIKSGQIKTLVKEGYNSAVTLAPNGRSCAFLQSRTT
                     SPDELFVYSFTDNKIRQITDFNSPLRSQIEMNDVEDFEFKSSDGAMVHGFLITPPFFD
                     AGEKYPAVFLVHGGPQSAWEDNWHYRWNPQLWSAQGYVIIMINPRGSIGYGQKFTDAI
                     SLDWGGQVFDDLVVGQKYVIDHFKFIDADKLAAAGASYGGYMMNWMEGHMEAFSYPFK
                     TLVNHDGSFNLYAMYLTTEELWFPEWEYGGVFWENRDQYEKFSCDNYIINFKTPMLII
                     HGEKDYRLDPSQGIMAFTALRRLNIPAKLVLFPDEGHFVLRPQNSRFWHQTIFSWLEK
                     YLR"
BASE COUNT        21703 a        16719 c        17379 g        23365 t
ORIGIN      
        1 ctaaaaaata atatttaaaa atgcaagtcg ttttttaggg ctgaggataa ggcaaaggct
       61 aataagagga cagaaacaga aaccacacct agctgtaacc gaatatctat taaacatttt
      121 tgattaattt gaaagcgcaa tatgttgtat aacactttgt aagttaaaat ttctgaaaaa
      181 tagccgtatt atagtgttaa aaataaattt ctatcaatgg tcaaaaaagc acattggaga
      241 tccaatgtat tcataagcca gtctaaagat agatgactat atttggggat aaaaaattaa
      301 tgattttctg cggttatcag acaatcatgt tctatccttc tgaatcggct gaacttcccg
      361 accaaaagga gttaatgcca gtgatagcaa tttgatatgc tgtcttgcaa agggaatacc
      421 gataatagtg atagcacaca atgcggcgaa aactaaatgc actaccgcca gctctaaacc
      481 gccaaaaagt aaccagataa tattcattaa agttgacaaa cagcccggtt cagatgcttt
      541 atagactatc ttttgtccaa acggcattag tcccaacacc gaaagcttga tacattgcat
      601 accgaaagga ataccgatga tggtcagaca tagcaccaca ccgccaaata aataaaagaa
      661 aaaaattagg attccgccac ctagaaaaat ccacaggata ttgcctaata ttgtcatact
      721 tactcccatt taaagactaa ctttaataca aagaaacagg ttttacgatt ggacatagtt
      781 aagtatcatt gcggctaatt gccctaacgt attccaccat accttaaaaa gcgatctcct
      841 ggcttaagct aacactcaat ccaacagcac cttaccggta cgatgaaaat caaacaatac
      901 ttctctaaaa acgataaaag taaccaactc atccatatta aatacaatat gcagatagcg
      961 catatagtct ggcacctcca gtccggtgct ttcgccgccg gtataggttc catcaatatt
     1021 gaattgttgc agaaattttt cgtcgcgtat tacagctgtc gggaaataat ggaccgcgat
     1081 ttgcctttcc ggatcaatgt ataaaaacgg cactggaata aaggtcttct gctggcaatt
     1141 ttcacagacc aacaggttta tttttccttc aaataattcc tttttcactt ctttgtcaag
     1201 agctgtattg agcgattgcc aaatttttac actttgttcg tgctggcaat gggggcaaac
     1261 gacgattttg gtttcattaa ttgacattgg attttccttt tagaattaaa ttattcgcga
     1321 attccaagtt tagcgaaaat aaaggcatat ttaaaggcta cttcctttag gtaatcatag
     1381 cgcccggatg ctgaaaaatg gcctttgccc atatcggttt taagaagtaa aacgttatca
     1441 tctgtctttg ttgcccgcag tttggcagtc catttagcag gctcccaata ggctacgcgt
     1501 ggatcattca atccggctgt gattagcata tgcggatagg ctttaggtcc aacgttatcg
     1561 tacggcgaat aggatttcat ataattgtaa tacttttcca gatggggatt gccccattcc
     1621 tcccattcaa tcaccgtcag cggaatcgaa cgatccagca tcgtattgat caaatccaca
     1681 aacggtacct cagctactac gacctcaaat aattccggac gcatattgac caccgcaccc
     1741 atcaacaagc cgccggcgct tccaccatta attaccaact tttcggctga ggtatatttt
     1801 tcggcaatca acatctcggc gcaggcgatg aaatcggtga aagtattacg ctttcttaaa
     1861 agtttaccat cttcatacca ctgacgtccc agctcgccac cgccgcggac gtgagcaatt
     1921 gcataaataa atccacgatc cagcagactt agtcggattg aagaaaaacg cgcttcgctg
     1981 ggaatcccat aagcaccata accgtaaagc aaaagtggat ttgaaccgtc ttttttcata
     2041 cctttcttgt aaactagaga aatcggcacc ttaacgccgt ctccggcaat cgcataaatt
     2101 ctttccgatt gatactgcgt ctgatcgtag ccgcctagca cctcatcttg ctttttcaat
     2161 tcacgagtct gactttccat atcataatca taaactgaat ttggcgtaac taaagacgta
     2221 taacggaatc gcaatatttt cgtgttgtaa tcgggattat tatcaccgtc aatggtatag
     2281 actggttcgg ggaaatctac gtaatgcatt acccctgtat ttaaattctc aacccggatt
     2341 ttttccagac cgttttctcg ttcatataca accagatggt cgcagaaaag atcaatgcca
     2401 gtcagcataa cattgggacg atgaggaatt ttctcctgcc aattgtgttt ttcgggtctg
     2461 gcaattggag tttccagcaa acgaaaattt atggcatttt cattagttaa aatgtaaaac
     2521 ttgtcgctat gatgatcaat gtaatattct acgccggtct tacgcgggca gaataatcga
     2581 aatcgtccat ctggctggtc agccgataga taatgaactt cggttgtaat ctgacttccc
     2641 aaggttagaa tgaggtaacc gtgactgcgc gtcttagcta ttccgagata aaacttctca
     2701 tctggttcgt gaaaaaccat aacatcattt tccggtgtct gaccgagttg atgacgccaa
     2761 agtttgtccg gtcgcagaat agtatcgagg gtggtataaa aaacagtacg gttatcgttt
     2821 gcccattcca gtccatagga aacatcaaga attgtatccg gcaacatctg gccattgatt
     2881 aggtctttaa aaaccaacat atgcttttct gaaccattga agtcaaccgc aaacgccagc
     2941 aaactatgat cgggacttac tttgaactct ccgattgaac agtatttctg cccgctggct
     3001 agccggttct gatcgagcag gatttcctct ttcgcatcca gcgaaccttt cttgcggcaa
     3061 taaatcggat actgattgcc ctttaccatg cggttgtaat agtaaaagct atctattttg
     3121 accggcacgg actcatccgt ttccttaatc cgggctaaca tttccttgta aagctttttc
     3181 tggaagcgtc tggtcggttt catgattgcc tcagtgtagg cattttcatc ttcgagatat
     3241 tggatgactt ctggattaga tttttccctt agccaggcat aattatcaat tagtgtatca
     3301 ctatgaataa tcgttgtttt gggttcgatt tttgccgtcg gcggagtcag cggtcgtgag
     3361 ggacagccgc ttagccagat tgccagggcg aggctaatag ctgatatact cgttcttaat
     3421 tgtttcctaa aattcatatt ctctcctgat tacttattgg ttcgctttgc caattcggtg
     3481 gtttcaaata aaatataatc cgtcttggac atacccattt tgcgataggt tttctgagca
     3541 atccggttat cgcgctcgac gtaaagccgc aaaccgcaga cattaccgac tttgagggcg
     3601 gcattctgaa catagtcgta cattttgcgg taaattccgc ggcgccggaa ctccggaggc
     3661 acatacacac tctgaatcca ccagaaaagc ccattacgcc aatcgctcca ttcataggtt
     3721 atcattagac agccaaccat ccgtccgtca ctctccgcca cgatataaaa accccattcc
     3781 gggtgctgga atagattacg cactcctgca gagattactt ccgcacttaa aatacggttt
     3841 tcagtctctt gcgccatcgc ccgattaaaa gcaaccaagt cgtccgcatc ggataaacgg
     3901 gcggaccgaa tcagaatttc cgcgttattt tgtgccatct atttttcgcc atttgacgga
     3961 attaatacta aacattattt ggatgatatg aaatagtttt cgtgctggca gaaaattttt
     4021 atctcagggc ggatagagaa ttcaattcca aaatgggaat cattaactaa agatacaact
     4081 attgccaaaa ttaacccgaa aaagcaatta gataaatcct gctaatcttg tttaaaacta
     4141 ttcacaacct tttatagaat actggttaat gaaaaaatta ggctttttcg aatcaagctg
     4201 attaactttt gatgattttt aatgtttctt atgcgcacat cggctaaaat aaatctgaag
     4261 ggacttaatc tggaagaaat cacttcactg ttagccgcat tgggtgaaaa accgtaccgc
     4321 gctcgccaga tttatcaatg gctttatcaa aaaagagcta ccgattggag tcaaatgtcc
     4381 aatattcctc tggcgcttcg tacgaagttg gctgagcgat ttgaattgag ctctgttcgt
     4441 ctgcaaaatt tggtgcatag ccggattgat ggttcggtga aatttctatt cgaactaact
     4501 gacggtaatc tgattgaggc ggtctatatg ccggaagatc gccgccgtac cgtctgcctg
     4561 tcttcgcaag tcggctgtcc aatcggatgt gctttctgcg caaccggtgt gatgggcttg
     4621 aaacgcaatc taactgccgg tgaaatcatt gaccagttac tttttatcaa tgccaatatg
     4681 cccgttccgg caactaatgt ggtttttatg ggtatgggcg aacctttttt gaattacgaa
     4741 aatgtcattc gcgccgcccg gattttcaat agcgagcttg ggccggaact ttccgcacgt
     4801 catattacaa tctcgacctg tggtgtcatt ccgggcatcg agcgctttac cgctgaagac
     4861 cagcgcttta aactggcaat ttccctcaat gccactaccg atgaacagcg ggataaaatt
     4921 atccctatca accgcaaata tcccataaaa gaactgttgg gtgtcgctaa aaattatact
     4981 cgtgtctctc gacgccgggt aacttttgaa tatatcctga ttgccggatt aaacgatact
     5041 ataaaagacg cccagcgctt ggtcaaattg ctcagcccga tcccctgcaa gctcaatctt
     5101 attccctata atgacaatca gcaatttcca taccgcgccc caagcgagga acatttaaac
     5161 gaatttatcc aggaaattta tcaggcaccc tttgcagtaa ccgttcgacg cagtaaaggg
     5221 ctggacatcg ccgcggcttg tggacaacta tatgttaccg aacaaaataa acgcaacttg
     5281 cgttaagcca gactggctac tctttgcctc caaaccgaac ttaagtcaag ccaaatagag
     5341 tggatgaact caagttctta ctttccaccc aattattcat acaaacaatc ctacaatcat
     5401 gcgttcaggc ttacattttt attatctggc taaaaatgtt tgcaatgatt atcaatgaat
     5461 ttgcaaaaat tactctttct tgaggctttc cttgactttc ttaacagctt cttcagcgct
     5521 cttctggatt ttatcgctgg ctttttctgc accgttctta gatacagatt ctacatcttc
     5581 ctgaatccgg tcaatgattt ttttcggctg agcgattttg tatgtcaagc caactgctag
     5641 cgccagaatg ataaatgaga tgactgccca cttgagcagg cgcgctataa ccattatgat
     5701 aaataacacc aatgcggcag tcagcgcgac tccgataatc ggatgagaag ttaggtagct
     5761 aatccacaca tccataataa tccctcccac tttaggttaa aagtaatccc attaattcgc
     5821 gaatccactt gcgctctgcc tgcaaaatga ccagcggtcg ggttaatagg gcgcgctgaa
     5881 cctggtcatt ggtatttgag agagcggtca gttcatgaac tttgttctga atctgccgac
     5941 ctagctcggt atcatagagc gttaacgcct ctcggagcgc ctccttatta aggacaccac
     6001 tataagttag accggtttca aaaagtgacg gctgatgttg tggttgggaa agggctttga
     6061 agatttcctc attaaaaatc tgacgcccct tgggtgtaat tgaataaatt ctgcggctgg
     6121 gtagtccttt ttgtggttcc aaattactca tcagaagacc aattttctgc attttgttta
     6181 aaacggcgta aatcgtcgag aatccaacgt tggtgcgttc gcgtatcccg cgttcggcga
     6241 taatcttctc aatattataa gcgtgagttg cgccctctga gacaatccca agtatgttga
     6301 gctccagatt agtcatcatt gttctccttt aaaaatgagc ggatacaacc aagattagca
     6361 tatatagttc ccgactttta ctgctccgga taaagtacaa cctctacgta gttatcaagc
     6421 ggcaggtagc gtcgggcggc attctggacc atttccttcg tcagatcttc tactaattga
     6481 ggataatgca ggatcgccat tggattttca ttgttgaaat aatagaaatg aaggttatcc
     6541 agccaaaagc ggttctcttt cagattagtc tcgtaggagc gtaaatcaat ttgcctaatt
     6601 ttggccagat cttcatcgcc aagtggttcg ctctttaggt tttcaatttc ttggaagatg
     6661 acggaggtta attctgtcac tcgctccggg tcgcaaccaa attgaatagt cagacgaaat
     6721 tcaggatatg ggtattgctt ggaagaggaa ccaacataaa caccgtatgt gccgcccttt
     6781 tcttctctga tttgttctcg caatttaatt cgcagaacat cggttaccgt tgctaattca
     6841 aaacgattct gacgactcca atcaaaatta ccgctgaagg taatctggac gtaggattgc
     6901 ttctcgagcc ctttataaac cgccttctgc accacgccgt tcggtggacg gatacctacg
     6961 tcgcgccaat tctcctcacg attatttgta ggtaaattag ccagatagcg catagccatc
     7021 tgtttcgccg aatctaattc cacattgcca attaggaaaa aagtaaaatc cccagcttct
     7081 ccaaaacgat ctcggaaaat gttgtatgat ttattcaaat ctatctcatc cagcatggca
     7141 atcgtccatg ggcgcgcccg gaaatgatac tgagataacg tcaccctgat tgtatcactg
     7201 aaagccgcaa atggatcggc ggctcgattg acaatcgagt ttttcaattt ttcgagataa
     7261 gacaggtagg cagtgctgtc tttgcgtggc gcagtaaaat aaagatatat aagttggaac
     7321 atagtttcca ggtcttcagg cgaggctgag gcactgaaat tttccgtcaa ttcctccaga
     7381 gacggttgca ccgaaaccac tttacctgcc aattttttag ataacatgac gctattgaat
     7441 tctcccaagc cgctttcagt gacgatggga acactgctaa ttgcggcaac ataatttgaa
     7501 tcgggaacca gtgaggttcc gccaggacta taagccgaaa gcagaatctc gtcattttta
     7561 aaatcagtcg gcttaatgat aacttttacc ccattactca atattagttc agtaagatcc
     7621 agggaatcaa tgagagtctc ctgcaggatg cgaccgggcg gcggcaattg cctgaccaga
     7681 tcgctttcag ccacctcatc aatataggct gtcacttcct gccgctccac cttttcgaaa
     7741 atttcgcgta attcggtttc agtcggtatt ttcaaatccg gtttctcggg ggcagatact
     7801 aaaactacgc ggttaatatc agtaacccat tctttagcca gaacattgac ctcgggcagt
     7861 gctatctccg gaacgtattt tttgtaaaat tcatattcga gttcaatccc cggtactggt
     7921 tcattatcaa gaaaattacg tatatattca ctggcaaaac tgcgtgagta aatcttatcc
     7981 cgctcgcggt aaatctgttc catattacgt aaagctgtgc ttttagcccg ttttagttcg
     8041 gtttctgtaa atccaaattc ccggacgcgt ttcaattccg taagaatagc cgtaaatccc
     8101 tcttcaatgc cattatcttt tacaacggct ccgatggaat aacaatcctt ggagcggact
     8161 aatcgcccct tttgagacgc cgctcctaga aaaggtggtt cggcagtttt ggttaattcc
     8221 cccagacgtt cattcagcat actgttgtat aaattttcaa caatcgattg gcgatagtca
     8281 cgtatatatt tttgttctgt tcgcggcaat ttaataagca aatccacttg attgcgggtt
     8341 agttcggggt cggtctgaat tgaaaacaag gtttcggtat gatcaggcac gggcggaatt
     8401 ggacgaggcg gcgcatcggc tggtgatttt aagccaataa aattttgctc gatgagattc
     8461 ttgaccctgt cctcatcaaa atcaccgacg gcaatgactg ccatcaagtc agggcgatac
     8521 cagtcgcgat aaaattttcg caatgtatcg taatgaaacg tatcaattac ggctatttgc
     8581 ccaattggat ggcgttcggc atataatgag ccccaaaaaa ttacctgaaa ttctttctcc
     8641 tggatacgca tttcggctcc gcgcccaagc cgccattctt cgcgaatgac gccccgctct
     8701 ttatcaattt cttcatcttc cagactaatc tgagaagccc aatcagccaa aatttggaaa
     8761 cccttttcca ataaagaatc acggtctgtc ggcacggtca gcatatatac cgtttcatcg
     8821 aaactggtat aagcatttag atgcgaaccg aatggcatac cggctaattc cataaaattg
     8881 ataatttcct gcttcttaaa acgggccgtt ccgttaaaag ccatatgctc cagaaaatga
     8941 gctaatcccc gctgatcttc ctcttccagc actgaaccgg cgttaaccac taagcgcagt
     9001 tctgcgcgcc tttccggctt ctcattttgg cgaatgtaat aagtcaagcc gttatcaaat
     9061 ttccctttgg taattttcgg atcaagcgca atttcagaat ctaattcaag agattgggat
     9121 aggctcggcg gagtaggcgg aatctttctg gtcaactcgc ttgtgctaca ggctatcatc
     9181 gtagtaaaaa gagccgctac gacgattgat tttggtatat gaactttcac gttgcctcct
     9241 tttataaaaa ttcgatttgc ttaataactg agcttagatc tccgataccg ctaattatgt
     9301 aatccttcga gatacttgga agcggacaga gctccaacag caccgtcgcc aacggcattg
     9361 gcaatttgac gaaatggctt ggagcgcacg tcgccaactg ctataattcc cggaacgttt
     9421 gtgtgcattt ctgcatcggt tattatatgt ccgtcatcat cgagattcac caagccacgc
     9481 acgaactcgg tattcggcac ccagccgata aaaatgaata cgccttcagc cggaaggctg
     9541 attttttcac cactcttgac attgcggatt tcaacgcctt cgacttgctc agctccaaaa
     9601 atattggaaa ccaccgaatc ccagataaat ctgattttct cattcttaaa agctcgctcc
     9661 tgcaataccg gttgcgcccg caatttgtca cgccgatgaa caactgttac tcttttagca
     9721 aatttcgtca ggtaaagcgc ctcctgaata gcggcatttc cgccccccac tacaattaat
     9781 tccgcattgc gatataatgg cccgtcacag gtggcacaat aggacactcc ccgtcccaaa
     9841 aactcttgct cggaggcgat gccgagattg cggggaaccc ctccggtagc aatgattaca
     9901 gctttagcaa gaaaacgacg attatccgaa attatttcaa agcattgcct tcctggtata
     9961 attttactca cgtcttcaaa ctcgctgata ttggcgccga aacgatccgc ctgagcccgt
    10021 atattttgca ttaattccat accacttatt ccatccggaa agcctggata attttcaatc
    10081 cgatcaacta tagccgggat gccgcccgtc atagattttt ctaataaggt agtgctgagc
    10141 atttcgcggg cggtgtagat agccgccgtc agtccggctg gtcctccgcc aatgatcaca
    10201 acatcgtaaa ccacatcttt tgccacagat tactcctttc ttttactatt ttgaaaatta
    10261 gttaaaatta tccatgctgg gttacaattt ttaataggcc ggttttaatt ttgatagcat
    10321 ttggtttagg cttaatatat ttataaattt tatcaaatgc atagcgaaca tgaaataatt
    10381 ttgattggcg atcgagtttt aatcgatcct gatgaagatc agaacaaaac gccaagcggt
    10441 ttattcttac ctcccggagt gaaagaaaaa gagaaggtgc agggcggctt tatcgttaaa
    10501 actggtccgg gtttcccaat cccccatacc gacgatattg aagatgaacc ctgggaagaa
    10561 ggggaagatg agccgcacta tttgcctctt caggctcaag aaggtgatta cgccattttc
    10621 ttgcgtaagt ccgctatcga aatcgaactc gaaagcaaaa gatatctgat agtttcccat
    10681 tctaatatcc tcctattgat ccgcgaaaac tacctcgaat aatactgctt ccaaatttca
    10741 tggtttaaca acttgggcat ggtattgcta aaataaaaat gcggtcgtaa agaccgcatt
    10801 gtgatctata tgcaacgaac ggagagcttt aatctaacca gttgatgtca acacttccaa
    10861 gtccaacctc aatttccaga tagattcgtt tgttggcggt ttcccagtta ccactgcggt
    10921 ataggttttc gctgatgcga tcaaaaccat gcaaattaag cgatgacagg aaggaacttt
    10981 gcgaacggac ttcaaccgcc acattatccg ggacttcgat aatgaccgaa ccgagtccga
    11041 cttcaatctt acctcgaata tcctgactca ggctaccgtc aaacaccaat tcggtagtgc
    11101 cgagaccaca ttccacgtta aagcgttcca tattggcgta actaagaccc ttaacattaa
    11161 cgctacccag tccagtctca ataaccatat tccgcatatt atcctggttc ttatctctaa
    11221 atatgacatt aacatcactc aacccgcaat gcaatttcaa atcccgcacc cgtaaatcgg
    11281 taaaatccag ccgaccttct cccaagccga gatccactgc aatgtaaatc ggtatttttt
    11341 gagaaaattc taaataccat ttatttaatt ctttatttac ttgggaagat ttgagatttc
    11401 ttttatcatt cttattggat tcgtcgtact tttcggtgga catttttagg cgaccacgat
    11461 tgcccagtat cttataatcc acaatcggct tataaatacg ttgtgaataa gtcattattg
    11521 aatgcaccag gtatttagga tcatcgtgac ttttgagatc aagagtcccc aaaccaaagc
    11581 tgatattaac gtccagctct tcttcgcctt taaacggaat atcataacgt tccgtgaagt
    11641 aatcttccgc tgataaaacc gctatcatga atatcaagag gataattaat tttcttttca
    11701 tattattttc atcacctcga ttcgcgatcg gcctttaata tgtttatgtt gccgttaatc
    11761 gtcctaagcc tgactagact gccaccttgg ttaattttgc ctctggcata gatgtatata
    11821 cgcgagcttt taatttcatg actcttctga accggaaaat cggagtggat attgacatcg
    11881 gcataacgac gactggtaac ggtaatttca gcatcaatat cggcattaag attagccggt
    11941 agaataactt ctatctcgcc gccgctggtt tccaaatcaa tggcattatt cttcacgtca
    12001 gaattaaata atttacgagc ttctatatca cctccgctgg tacgcgcata gatgtatccg
    12061 acgactcctt tggctaggat atcacctccg gaagtaatgg cttccagatc atatgcccgg
    12121 tcaacctcaa tattgccacc agaagtacga gcataacaat aaccttttat gagtccaata
    12181 tcgatgttgc cgccggaggt agtagccttc aaatttttat ttacctgacg cacttctata
    12241 tcacctccag aggtgtgaat ttctaaattg ccttcaatct ggccaatgat tatatcaccg
    12301 ccggaggtat gcagggtaag ctcagcctca actttatcgg caccgatatc gccaccgcta
    12361 gtcttagctg aaaaggtttt cgcccgtaga ttgacaatat ttatatctcc accagaagtg
    12421 ctgacctggc cattaccctg gattttatca aggtttatat cgccaccgga agtgttagcc
    12481 cagagttcgc catcaaataa ttcgatctct atatcaccgc cagaagtgtt gacggataaa
    12541 ttacctgcaa cttgttgaat tttaatatca ccacccgagg tatggctttt caattcacca
    12601 ctgcattcgc taatctcaat gtcgccgcca ctggtattta gattctgaag tccactaacc
    12661 ttattaatat taatgtcgcc acccgaagtc tgaactgtcg tattaaaagt caagggcact
    12721 ttcactataa aatcgctgga gtagcggcgc gaatgttcct tgccagtgac tattacttta
    12781 ttgcctcgtt gttgatagcg tgctttttcc tcatttaaaa tttggcgcgc attaacctca
    12841 gaataggcgt ctattttaaa cttctcgacg atcatcacgt cccggcgagc ctcaccctcg
    12901 atcgtgacat ccccactgac gcgctccata acaagatcgc ccttactacc gactgcaaaa
    12961 gttttaacga tgtttccgac atattgcttg ccatcaaatt caaatgtgtc ggggctggcg
    13021 ccgactgagc cgatcatcaa tagaattagg aataattttt tcggtttcat cgaatatact
    13081 ctctatcttt tttctcgatc gtttttatca taaaatttgt ctaaagccaa aagagccatc
    13141 tgtcttattt cttcagattc atcacgcgcc gccaccaaag ctaatactgg cacaacttcc
    13201 tgctctgcct tattaaccaa attttccagc gctattttac gaagagatgc gcttgaatct
    13261 cgcagggcaa ctaattggaa tgcttcctga atttgcttgg tcatctgtcg tttctccaac
    13321 aattgggcgg caaataagcg taccgattca ttattatcat ggagcatagc cgccacaaaa
    13381 gtagcgtcaa cgatagggtt gggttcgatc tggtccaata ggcgcagtgc caaaaggcgt
    13441 ttttcggcat cttgattatg acgggcggca tacattaaca tgttttgaat ggtgggatca
    13501 tctaatccac ctctaatggc aaaatcttcc tttactctga cattaacttc tactaatcca
    13561 ttctggctgg ttcgatattc actttgaagt aagtcagcat cttctatccc ccctgatttt
    13621 agtaaatagt tagtaaaggc tttttgaata ttttctttct ccggatcggt gaatttatag
    13681 ttgtaattaa caaagttggc aagtatttcc ggttgtttgg gtaagtcggc tctaaatcct
    13741 actcttccaa taaaaatgcc gattactaga attaaactgg cggcaattag ttgataacgc
    13801 ggacgggaca agccgattgt aatcgtctca aacatttcct caaaatatgt tttgacattt
    13861 accaatttcg gtcggcgcga ttcggatgaa atttttgata ttacttgctt gttcaatgct
    13921 tccaatcgtc gggcgctggg caccggttca ttagcgtagt ctattttctt ggcaataatt
    13981 tgtagacgtt ctatttcggc ctggcagact tggcactcgt ccagatgatg tttaaaatca
    14041 gttcgttcgg attccgatag ttcattgtaa aagtatagga ttgaaagttc tttaaaatgt
    14101 ttatcgtgct tcatatctaa atcctttctt atgattcagt taaaagcgtt tgtaatttgt
    14161 gagttgcacg gaataagtag cgcttgacgg ttccctcggt gcaatcgagc aaaatcgcaa
    14221 tgtcctttat ttttttccct tcaaaatgct taagaatgaa tactactctt tgttttgcag
    14281 atagttgctg gagagcggcg tctatttggt cctgaatgga attgctgaat gttacccgat
    14341 cggcttcgtc ttgagcgtcg gcaggaatgg tctcccacag gtagtcgtct tcaccgggat
    14401 cagctaaagt catacgtttg cgtttattat atagattaaa agcttgatta gctagtatgc
    14461 gataaatcca ggtaaaaaat tcactctcga agcgaaattt tcccagattc tgatagactt
    14521 taataaaggt ctcttgataa aggtcttccg catcctgata gttgcgagta aagcggaagg
    14581 ctacactcat cacccgctcg ccatattgtt ccattaaacg tgcgaatgcc attttatcgc
    14641 cactttgaat attctggatt aattcgactt cttcgttttc catacatcta cccgctttag
    14701 attcacatcg gcgataaagg ttgtcaaact tctgtttagt ggacaataat gaatttattt
    14761 attttgaagt attcagagct tcgtaggcat acaaagtaaa ttccagagcc aacgcgcgcg
    14821 ccggattgat tatgtaaatt ccaattgatg acttcttttt ggccgccaaa tttgctaacc
    14881 agcctttttt caaaaacgat ttgtccgttg agatttatga cttgtaggta agccggagcg
    14941 gtagcgggcg tggctttatg ccagataaaa taaaaattta tgttgctatt agccgggttg
    15001 gggtaaaccc aaatctcatc gaaagatagt tcgcctttcg ggaaactctt gggttggtaa
    15061 ttgacgatac tttgatagac atctggaatg ccccagccgt atctattatc tgggttttgg
    15121 gtgcgatcgg cataacgttt cagttccgtg ataatttctg cgggggtcag ctctggctga
    15181 ctctccaaca atagcgccgc ggcaccagca atcaacggtg tcgccaagga agttccgctc
    15241 cctgtacgat aagctccttg cgaattaccg gcgtaataag tcgcaacgcc catagcgcac
    15301 aaatccggtt tgattcggcc gtcgtaggtt ggaccatggg aactgaaggt ggctatttca
    15361 ccctgcgcat caaccgcacc gaccgtcaat gcgccaaaag catcgccggg tgtgagcagt
    15421 ccgccatcct caaaattatt agcgtcgtta ccagcacaac agacgcagag aatgccacgc
    15481 tcgaacgccc agttaacagc cttagtggtg actgcggttt ggccatcaag gtattcataa
    15541 ggatatttag aaccatctgc aaaaccatca cgatacccga gagataccga tacgatgtca
    15601 gccccccaat aatcacacca ctccaccgcg gcgacaaaat catcttcctc acgatgaatt
    15661 tcggtatcaa ttctttcggt tttggctagt aaaaattcag cgccataagc cgctccaagg
    15721 taatcaggtg gattatatcc gccgactaag ctccagacgg cggttccatg ttcactctgc
    15781 cggctgtttc gggcggtatc cgcggctttt tcatcgctga cgttgtcatc atcattgata
    15841 aaatcatatt cggctatcaa gcggccggaa gatataattt ctctaaaaac ttcgtggtct
    15901 ttataaaacc cggtgtctat aaatccgatt cgcactcctt ttccggtgag ccccaaagct
    15961 tgcgccgcgg gaatattaag ctgttcaagc tgggtttcgt aggcggcact catcttccgc
    16021 aatggctttg tccttgattc tgggatttct ttaagtaaat ctggaggttt agcagtaaaa
    16081 ctctcaacca gctcgatctt tgtaacaatc ggtagagagg tgagaatctc aaaattactt
    16141 tggtttattt cggcactgaa cgcccgtaaa attcgggagt agtgtcgtaa acaaatgaca
    16201 ttcgagccaa taatagcccg taattcttca ccgggatcaa ggtcagaagc ggtcttcttg
    16261 gctaacgttc ccattttggc aaggcgtcgt tgagtttttt ccggatattc gagttgcgta
    16321 aaatattcat tataggctgg ttcgttcgct aaataaatcc aggctcgtat agttggttga
    16381 ttggtgaatt tatctattgt aaccggttgc ccagcccgta caatgccagg aaggattaac
    16441 agcaaaaacc caaacacccg aaaacgaaga acatatatgt tccggatgcg ctgaatcatt
    16501 tcggcaggca atctattaaa atacgagaaa ataaaaaatt gttattcttg aggaaggcgt
    16561 tggagacgtt aaaatcagct accaatccag gtgagcaagt aacaaaccca attgtttacc
    16621 atcaatatct gaaaataaaa ttagtccagc gcattgacgt ctagcatgcc taacggttat
    16681 cgctccggcg aaaacatcct gagaaagtgc ggcaatttcg ttttgtacta tcaatttttc
    16741 ttccagagat gtcgagctgg tcattgaaac gggtacaagc agaatacctt tctgtaaaac
    16801 gagaccggct tgtttaccag cgataaggac atcctcggtt aatttcgggc tgtacaaatc
    16861 ggtcatatct ggaaaacgcg tcccgaattc ttcatcatta ggaccaacca agggattctg
    16921 cccggaaaca ttaatctgat ctacaactac tattcctgcg gacttttcgg accgccgcgt
    16981 tggcacacct cgtccaacca ataaaaaacg ttcagcgcca tctttaatca ccgagcgaac
    17041 ggtttcacgc caggttttaa catttgcccc attatcgaga agaattatgt taaagtttaa
    17101 ttcaccctct ggtatttgcg ggcatatggc tttattcatt ataagtgcgt tatgaatttt
    17161 ttttagagac ataatttttc aggttgtttc ctcagatgac tcgttaaggg tgttatcttc
    17221 ttccgaccaa tctggtgctt cctcgagcca ggtaagtatt tccgcctgat ttttcaagac
    17281 acttcgtaat cgttgtatca atttattctg ttcgaggttg agctgatcaa ttgcctcctg
    17341 tatttgcctc ttcttggttt ctgcattggc aattacttca gaagcaatgg attcagcatc
    17401 caagataata cttgccgccc gatcttgagc ctcttgcaaa attttatcgc gtcctttgcg
    17461 agtctggtca aactcattta ctatggcttc caatttctct agcttttctt ccatctgagc
    17521 aaattcatcc gccaacattt cggtaaaata tttaacctcg accggatcat agccgcgaaa
    17581 tactttgcgg aattggcggt cgcggatttc attggtcttg atcatgatat tctgcgctcc
    17641 ccaaaaatag cccgtccgag gcgaatcata tttgagccct cctcgatagc aatttcataa
    17701 tcgtcggtca ttcccataga aatgagattg aattgaccgg tattaatccg actctcagca
    17761 attttatcaa aaatgccttt aagattggca aatgctcgcc gagtccaatc ccggtcgttc
    17821 gttaatgggc caatcgtcat taatccgtgt aaaatgagtt ctggcatctc cgcaacttgt
    17881 tccacaacgg aaaatacttt ttcgggaggt aaacctgatt tagtggcttc gccggaaaca
    17941 tttacttcga gtaaaatgtc caccttccgt cccagtatac ggctaatatt ggcaatttta
    18001 ttacacaaat caagcgagtc aaccgattga atcatagcaa aattctcgat agcctttcgg
    18061 actttattgc tttgaaggtg tccaatcata tgccagctga tttccgggca atcttgcaag
    18121 gctattattt tcgggatggc ttcttgaacg cgattctcgc cgaaaatccg taaaccggcg
    18181 gtataagctt gacgaaccaa tgttgctgga aatgttttgg ttacggcgat aatctggatg
    18241 tcttcggctc gtcgccctgt tttttgagcc gcttgcgcaa tgcgttcttt gattatcaaa
    18301 aggttatttt gaatggtctg cgagtccaag aaggctaatc cttttagatt agattactct
    18361 ggttcatcag tttcggcaac ctcgtcagac gcgtcatttt cgagcagggt aatttcatcg
    18421 gattcttcca actcgccatt gccgttgttt ttatcttctg attccaccac gcgagtaacg
    18481 gctgaaatgg tgtcaccttc gtccaatttt attagccgaa cgccctgagt attgcggctg
    18541 atggttctga gagtagcaac tggcaggcga atcaatacgc ctttggcggt tataatcatc
    18601 aaatcgtcgg tatcaaccac ctcgcgcaag gctaccatcc gaccgttgcg aacggaagtc
    18661 ttgagtgtta tgactccttt gccacctcgt ctgatgaccg gatattcttt gatgtcgctc
    18721 cgtttgccgt aaccgttttc agccacggct aaaattgtac cgctttcatt acgaacaatt
    18781 acaacgccta caacccggtc atctttgccg ctcaaacgaa cgcctcgtac accggccgcg
    18841 gtgcgtccca taggccttac gtcgctttca tggaaacgga ttgacttgcc ataagccgtc
    18901 cctaaaataa tatcctgatt accatttgtg atctggacat cgagcagatc atcgttatca
    18961 ttgaggttaa tggctcgaat gccgggttta cgcggattgc caaattcgga caagaccgtt
    19021 tttttaacta taccgttgcg ggttaccatt aggacaaaat gttgatcgtc gaattctttc
    19081 actcccagca ccgcccgaac ttcttcgccg ggctgagtat tgatcaaatt gacaatggcg
    19141 cgaccgcggc taccttttcc ggctcgcggg atttcatgaa ctttcagcca gtaacagcgt
    19201 cccttattgg taaaaactaa aagataatca tgggtattag caatgaatag gttgcgcaca
    19261 aaatcatcgt ccttagcctg cgctcccaga ctgccgcgtc cgccgcgatt ctggcgccgc
    19321 gaagcatcaa ccgggaagcg cttaataaat ccattatgag taatagtaat caccatatct
    19381 tcttcggcaa tcatatcctc gaccgaaaat tcgccctcgt ccgtaataat ctgagtgcga
    19441 cgcgtatccc catatttttc ctttacttca gctagttcat ttttaattat caacatctgt
    19501 tcactaacat tgctaagaat gaactttagt ctttcgataa gtttgagagt ctctttgtat
    19561 tcctcaatga ctttggtgac ttccaagccc gtgagtcttt gtaaacgcat atccagaata
    19621 gcctgggctt ggcgttccga aaatcgaaag cggatcataa gattttgctt ggcatcttcg
    19681 ggagactttg atgctttaat aatagctatt acttcatcga tattatcgag agcaattttc
    19741 aagcccttga ggatatgtgc cttgtcctcg gcttgcttaa ggtcgaattg tgtccgcttt
    19801 atgataattt catgccggaa atctataaag tgttgtagaa tttctcgcag agacaactgc
    19861 cgcggaacgc cgttgaccag cgccagcata ttgattccaa agctttcttg taattgagtg
    19921 tggttataaa gctggttgag gatcacttcg gcataagcgt ctcggcgtaa ttcgatgacg
    19981 atccggatgc cttccttatc ggactcgtcc cgaatatcgg ttataccgtc tatgactttt
    20041 tctctgacta gatcggcaat cttttctatc aaattagact tattaacctg ataaggaatc
    20101 tccgtaatga tgatgctttc cttaccggtt ttgctattct caacgaaagc ctgggcgcgc
    20161 attttaatta cgcctttgcc ggtttcataa gcacgcttaa tttcactttt accgaggata
    20221 aaagcacctg ttggaaaatc cggacctggg ataaattgca tcagttcgtc taccgttgct
    20281 tctggatgat caataacata tatagcggcg tcgcagactt ccgttaaatt atgcggcggt
    20341 atattagtcg ccataccaac cgcaataccg ctcgaaccat tgaccaaaat tactgggacg
    20401 gctgttggta gaacacttgg ttcttcaaga gactcgtcaa aattgggtac aaatttgact
    20461 gtttctttat caatgtcccg caataattcg gacgcaatac gactcattcg agcttcggta
    20521 taacgcatag ccgccgcgct gtctccgtct aacgagccaa agttcccttg gccatctact
    20581 aatggataac gcatcgagaa aggttgtgcc atccggacca gcgcatcgta aaccgccata
    20641 tcaccatgcg ggtgatattt accgagcact tctccgacta tacgagcgca ttttttggtc
    20701 ggacggttat atgttagacc caactccgac ataccatata aaattcggcg gtgtaccggt
    20761 ttaagtccat ctcgtacatc cggcagtgcg cgtgctacga ttaccgacat ggaataatcg
    20821 aggtacgaac tgcgcatttc ctcatcaata ttgaccggta cgattttatc tctttgaaat
    20881 tcgatattta cttgtgatgc cattttttaa atatccaggt tcttaacata ttttgcattt
    20941 ttttgaatga attgccgacg tggttcgaca tcaccgccca ttagaatcga gaaggtacga
    21001 tcggcggcgg cggcttcttc tatcgtaatc tttaacagag tgcgagtctc cggattcatc
    21061 gttgtgttcc acaactgttc tggattcatt tcaccaagtc ccttataacg ctgaatatct
    21121 atcttggcag tcgaatcctt cgaaaatctc ttggtcagaa cttcaagctc ttcatcatcg
    21181 taagcataat attcttgctt tttacataca actttgtaaa gcggtggttg agcgatatag
    21241 atatgtccat cctctattag tttgggtaga taacggaaga aaaaggtcaa cagcagggtg
    21301 cgtatgtggc taccatcaac atcggcatcg gtcatgatga tgactttgtt atatcgtaac
    21361 ttttggatat cgaattcatc gtcaccaaaa ccggttccaa gagctgtaat aatagtacgg
    21421 atttcctcat tgctcaaaac tctgtcaata cgggtttttt caacgttgat aattttgcct
    21481 cgcaacggca gaatagcttg atagcgccga tcgcgcccct gtttggcact accccccgcg
    21541 gaatcacctt cgaccagaaa taattcgcac attgatggat ctctggtgga acaatcagcc
    21601 agttttccag gaagattgcc gatatccagc gcactcttac ggcgcgtcaa ttcgcgggct
    21661 ttacgggcgg cttcacggga acgagccgca ttaagacttt tctcgataat acgacgcccc
    21721 tgagtgggat tacgttccag atattccgcc aaactttcgg aaacgatcga gtccacaatg
    21781 cccttaactt ccgaatttcc gagcttagtc ttggtttgcc cttcgaattg gggttcaggc
    21841 accttaacgc taatcacagc cgtcaaacct tcgcgtacgt catcgccaga caactgaaat
    21901 gactcttttt tgaaaagatt gtttttctgg gcatagttgt tcagggtgcg agtnnnnnnn
    21961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatgactc ttttttgaaa agattgtttt
    22081 tctgggcata gttgttcagg gtgcgagtta aagccgtctt aaaacctact aaatgagttc
    22141 caccttctat cgtgttaata ttattaacga aagtaaaaat attctcatta taggaattat
    22201 tatattgcat cgctaattca acgaaaacgc cgtcctcttc cttttccgca gttatcggtt
    22261 tatcgataat agccggccgg gtttcatcca aaaaatttac aaattcagct agcccacctt
    22321 tataatggaa cctattctcg tttttatcac gctcatcaac caaaacgatt tctaaatccc
    22381 ggtttagaaa agcaagctcg cgacaacgat gctcgagtac ttcccattca tactctattt
    22441 tcttgaatac ttcaacatcg gctttaaacc ttacgagtgt cccattttta ttagtcttgc
    22501 caactggttc aactttactg gtgggtaccc ctcgtgaata actctgagta taaatcttgc
    22561 cgtcgcgata tacttcgacc ctcatccatt ctgaaagcgc gttgaccacc gaaacgccga
    22621 caccgtgaag tccaccggaa accttatagg aacctttatc aaatttacct ccggcatgca
    22681 gtatggtcat cacgacctcg agggctgatt tcttttcttc tttatgaata tcaatcggaa
    22741 tgccgcgccc attgtccagt acgctaacgg aattatcttc atgaaccgtt accacaatct
    22801 tattgcaaaa accggctaat gcctcatcaa ttgaattatc aataacctca aatacaaggt
    22861 ggtgcaaacc ccgtttgccg gtgtcaccaa tgtacatagc cggtcgcttt cggactgcat
    22921 ccaaaccttt aagaactgta attttttcag ccccgtaatc aattgcggcg tcggtaaatt
    22981 taatttcgct caccttaaga taatctcctt aatctgtgca ttttttattt gactgtttat
    23041 ccgctttaat atttcttcct tgcgaaagtt taattcattg cgccaaaccg gcgaatcaac
    23101 tttgacaaat aatttattat aggcaacttt ttctggtatg gcatgcttgg caatgacttc
    23161 acccacaatg tcttgccaga taaaaaaaac ttcattctga cgaaccggct tttctatgcc
    23221 ataaattttt agcattctgg caatagccgt accgagagac tccatcttta tgcttcccca
    23281 gttgtcagat taaaaatttt atatttttca ggattatcaa gaaaaccata ctttcgcaac
    23341 attttgacat cggttgaagt aatcagcgcc tgatttttat caccgatttc ttcaacgatt
    23401 ttacgacagt gtttaccatc taaaaaggca aagagatcat ctaataaaaa tataattttt
    23461 tcgttttggt ggctctcaat atacgctcct tcagccaatt ttaaggctat aagaattaac
    23521 ttatgctcac cctgagagcc gatctgacga atatctctgc cgttataatt gataataatt
    23581 tcatctaaat gaggtccaca ggttgttcgc ccgataagta agtcgcggct gaacctttct
    23641 cttaagtttt tggtaaacaa agaaataaaa ttgtccgggt cggctttaat attatatttc
    23701 agatcaattc ctaccgttcc gccaatatgg gtaattcgcg caaagactgt tttgaaaatc
    23761 gagtccatat cggcaataaa tgcctgtcgt ccaataaaca acttttgccc cagtctggct
    23821 aaaatttcgt cttgggcttc aaaataagca tcataacgac agcgtttttt ctctcgatac
    23881 tggttgagta cgacatttct ttgtgagatt ctacggcgat attctatcaa agttaccagg
    23941 taattgcgat cggtttgcga aattatccga ttaatgaaat tacgacgttc ggctgggccg
    24001 ccttccgtaa ttttctgtaa tcctggtgat aatatcacta ctggcgcaca accgacgacg
    24061 tcggtgtatc tttttaccct gcttttattc atcaaaaaac tgcggccttc agttttagaa
    24121 taattcatat tagacaaaat tgtctgattg ttatcgctta aaaactcacc aaaaatattg
    24181 aagtaatcac tttgataatt aaccgcttcg ctatcattat tcgtacgaaa acttttggtt
    24241 agggataaat aatggatggc ttcgagtagg gaagtttttc cactaccatt atctccattt
    24301 ataaaatttg tcttttcgtg aaatacaaaa acggaatcgg tatattttct aaaatttaca
    24361 agtctcaaat ttttcaatac cattaataat catcttttac ggaaaataaa gagaggtgtt
    24421 aatcacttag cctgatcggc attaaaagca ttattaaatc ttcattttct tcttgattct
    24481 ccgggattac caggctggcg ctgaccggag tctttaattt cattataact ttttgagtat
    24541 caatattcct aacgatttct cgaagatatt cggcattgaa tccaattgta agatcgtcgc
    24601 cactatattc gacatcgagg atttcatctg ccgacgacat tgtttctaaa tctatcgtgc
    24661 ttattttaga cagttctcga ttcaaactta gcgatatttg atgggttgat ttattagaga
    24721 atatagacac tcttttcaaa accgctgaaa gataatctga atcggctttc agctccttat
    24781 cattatcttt gggtataaca ctttcataat ccggaaaacg ttcgtctatt attctggaaa
    24841 ttatcagggt tgaatcactt tccatcatta tgtgattgtc gcttatagtt aaactaacct
    24901 tattatcatc ggtaaggtag gtattaatta gattgagaaa tttaaccggg actatgatct
    24961 gtcgttcata atccggcgaa ctgaaatctt ttctattaat atataccagc cggtgaccat
    25021 ctgtcgctac cgatctaagt tcgtcagctt tgacttcaaa aagtacgccg gtcaaagacg
    25081 gtttaagttc atctctacta accgcaaaca ctgttttttg aataattctc gataaaattt
    25141 tactgttaac taaaatacta ttttgattaa taatacttgg caaagctgga aattcttcac
    25201 ctggccttcc cataatttta tatttacctt ttcctgcagt aatttttatc tcgccttcat
    25261 cggtagcatt aaaggttagt tcaccctctc taatctcacc tactatttct tgtatgagtc
    25321 gagatggaac tgcaacactt ccatcttctt gcccgttcac tggaaaagtc gtacttaagg
    25381 taatttcaat atcggaagct cttagtgtta aattagaatc ttttaagata aaaagaatac
    25441 tattcaatat cggcatagtt gttctggcgg gtgtcacttt ggctacctta tttagattat
    25501 taagtagtaa attttggcta acagaaaact gcatattcta tctccttact tttaatattt
    25561 tcataacaag attttatcaa agaatactgt tttttccaag taatttaaaa caaataatca
    25621 aaagtaattt ttacatttta agtaactttg aattattgaa atattagtaa ttagataaag
    25681 gatgttaatt tgttaataac atgtaaaaat tagatgattg agaagatcgt taattgtaaa
    25741 atacactgtt gataatttgt taacgttcat aaacaggggt aaagtatttg tcgtcgtgat
    25801 ctaaggaaag accaatgcac cttgttaaca aataattaaa ttagtgttaa cctgttaata
    25861 atttaccttg atgtcaataa agatgtcttc ttttagctta tttagccact cttgatacag
    25921 ttgatttttt ttaaattcca gtgcaaatct ttctatcgtt ggccaactat catcataaac
    25981 gttattaagt tttaaaatgt aatatccggg tgacgactca aaaaccggac taatatctcc
    26041 aaccttcata ttacttaaga cattaataaa atctgtattt tgaatctgat ttagcggaat
    26101 gcgtctaata cgccctaagg tggatttggc attaggttcg tcgctatatt ttactacagc
    26161 agaatcgaat gaaatttcat tatttataat catagtacga atactatcag cgaagcgata
    26221 tcggtcttca tagtttttat cggacacctt gattttgatc aagatatgac gaaccctaat
    26281 gctttcacct tttcggtcaa ccatttggat gatatgatag ccgaaatcgg ttttaattac
    26341 gtcgctaatc tcaccgggcg agagtgaaaa agccacctct tcaaaactct taataaatgc
    26401 gccacgctta atatacccca agtctcctcc ataaggagca cttgcctcat cctccgaata
    26461 atttttagcc agatactcga aatcataccc attttttagt aaagtataga gcgaatcaat
    26521 taatttttta gccgccattt cttcctcggg accaggagtg actttgaata atattgcact
    26581 aaaatccagt gatggaggaa tcgggggaat actgtccttg taggtttgat aaaaagcctc
    26641 tacttcagga cgcgtgacgg ttactttact aaatttttcg gaacgcattt tctcgacgat
    26701 cagctgattt ttaaaaatcg gacggtaatc tttttttatt ttactgatag gactgcccat
    26761 caacaattcg agattttcta ccgaaccaac ttgctcaatg agcatatcca aacgttggtt
    26821 aatcgcattt tccacatcgc gctctttaac ttccaccgtt tcaattctgg cttgttccag
    26881 aataacattc tcatcaatta aagaatttaa aacatgacgt ttgattttat taaaatttgc
    26941 aacgtcgcgg ttaggatcaa tattcatctg agaagccgtg attcgcgcca cctgatcaac
    27001 ctcacttatg aggataatgt tctcgccgac aattgcggcg ataccgtcaa tgagcgtttg
    27061 agcgaaaaga gtggtcgcca aaaagataat accaaaaaat aacttaattt tcatcgagag
    27121 cctatagagt ttaaaaaatt ggaaatattt tcatctttta tttcataatt agcattccgg
    27181 acaagactgt caaccagtgc gttatactga tcttgaattt ttttctgagt caatatctga
    27241 tagatttcat cacgaacctc atcgattgtt cgcgttgagc cgataggata gcgggctaga
    27301 acctcaacga tatgaaaacc ataatttgat gcaatgggac caatgatcga acgaggagaa
    27361 gtttcaaaaa tattacgatc tatttcttcc aaagattcac ctcgcttgac gacagaggtc
    27421 tcaaatttat acttactata aaacgtatca agcgcattct tattgcgact agttatagca
    27481 gattttattg caagcgcatc gttaaaatcc tgcacaagaa catgaacgat cttagcttct
    27541 tcacgatcgc gaataaaact ttttttgttt ttaaggtaat aatcacgtat ttcgtcttca
    27601 ctaatagtta cattggtttg taagaaaaaa cgtacatagg aatctatagt taaatcacga
    27661 taataatctg ccacccgggc ttgtacgcct tcttcgcggt caaaatgata ctggcgagct
    27721 ttttgataaa gtatctcctt gtttatccag ttggaaacaa cagagtttag gtaattttta
    27781 tcaatatctt tcgataagcc accgtcgggt aacattcggg ttaagtcttc attggttaaa
    27841 taagcatttc cgacgcgcgc aatataatct tctttatgat tgccacaagc cgcccagaga
    27901 aaaatgaata tcattaataa aaagtccttg cgcaatttta tacctcccaa gctaagttac
    27961 ctatcataaa caaactaaaa tctatgcttt aacaatcaaa cagagattta gttctagccc
    28021 aaaacctaca tttttagaat aagaatacat tttcatgact ttttatactc ctaaaacgat
    28081 actataagtt aagggtgttg cgaagcagat tcaagaagcg agtaatttta tcaaaatcaa
    28141 aattactttt taaataaacg gttaaatata aggtattgta aggattaaac ttataagaca
    28201 aacccaaatc agaagcggcc ttttggatgg cttggatgag atcggtttcg gctggcggta
    28261 taaaagattc gtcccagata aagctggttg atttttcggc aatattgact ttttttattc
    28321 caagtgatga agcgaggagc cgaatcttgc tgatattaaa aagagtcgtt gcttcagacg
    28381 gtaactttcc ataaatatcg cggatttgtc ctgagattaa atctactgta gacaattcat
    28441 ccgccagtgt tagttggcga taatattcta aacgtgccga agaatcggaa atataactgt
    28501 caggaaaata ggctggaagt gggcaaaaaa cttcagtttc caattttaat ttttctgggt
    28561 aagcgggctg ggttgatttg ttctcatcaa gcgcctcgcg caaaaatttc atatagagct
    28621 catacccgat cgccgctata ttattgccgc tttgcgaggt acccagaagg tttcccgcac
    28681 cgcgtatttc gagatcttga agcgccacac tataaccaga accgagtgca gtatgttttt
    28741 taatagtctg gagctttcta agggcgtccg gtttaatgcg agacggttcg ggaacaatca
    28801 agtaagcgta agcccgacga ctactcctgc caacccgacc gcgtaattga tataattgag
    28861 ccagaccaaa atgatgggct tgattaataa aaattgtatt agcgttagga atatcaatgc
    28921 ctgattctat aatggtagtg gtaaccaata aattgatttc attgttaatg aaagccgaca
    28981 tagtcttttc gagttctctt tcattcattt gaccatggac ataatctacg ctgatttctg
    29041 gtagagccag agcaagttta tgactgagag cggcaataga tttgatgtca ttatgcacaa
    29101 aaaagacttg accgccacga ttaatctccc gcaagatagc ctttttaata atattcatat
    29161 cgaaagtgat gacttcggtt attactggca agcgagagat tggcggggtg ttgataacag
    29221 aaaagtcgcg tactccaatc aaagaaaaat ttaaagaacg cgggatcggg gtagctgata
    29281 atgccagaat atcaaccgat tcgcgtaaaa atttaattcg ttctttgtct ttgacgccaa
    29341 agcgatgttc ttcatctata actaataaac caagatcttt aaaactaaca tcttttgaca
    29401 gtagtcggtg agtaccaatg acaatatcaa tctttcccgc ggcaagatca cgaataatct
    29461 gtgactgcat tttgtgggac acgaatcgcg ataaaagtgc aatttttatc ggatattttt
    29521 gaagccgctg gctgaaaaca gcaaagtgtt gatcagccag aatagtggtt ggtaccaata
    29581 ccgctacctg cttggacgcg gataccgcct tgaaagaagc ccggatggct acttcggttt
    29641 ttccaaaacc cacgtcgcca cacaaaagcc gatccattgg acgaggtttt tccatatcct
    29701 ctttaacggc ttgaatagca gtcagctggt cgggagtttc ttcatataaa aattccgatt
    29761 ccattaagta ttgcagttca ttgtctggtg gaaaagcata gccgggcgcg cttatccttt
    29821 tggcgtaaag ttcaactaaa tctttcgaaa tttcatcaat agcccgttgt gtttttaatt
    29881 tggtgcgttc ccattccgtc gtaccaagtt tattgagagg tggcggattc tgaaggtttc
    29941 cggtatattt ctgaatatag ttaagcttct caagtggtac aaatacttta tctccgccag
    30001 aatattccag gcaaagacat tcacggatcg aaccaaaagc cgcgatttta tccaatccag
    30061 caaagcggcc aactccataa ttaatatgaa ccatcaaatc gccgggagat atttcgtgcg
    30121 gactgaattg ttcaacagga aagtctgggg aatacttttt gaaaaaattt atccgtcgtt
    30181 cacgcttgaa aatttggtga tcggtataaa aatacaactt tgcttcgggt agttctgccc
    30241 ccgccgagat agaaaaatca aagtagttca atattgccaa atccaataat tccttaagac
    30301 gtttcacctg catagaatta gcacaaaaca acgagattcg ggcatctggc tgggcagaga
    30361 gcactgtcaa taaatgttct ctaaaagctt taattgaatt ggtttgcggt tgaggattca
    30421 cccggaaatc aatatctgaa gcaagtagat cgtaatgatt aattacccta tatgatgata
    30481 gataagtaaa tatatcggtt gtgttttttt catctgtatc cataagtttt tcagcatgaa
    30541 ggcaaaaaac cacagtgtct tttttaaggt aagaaaaaat attagtagag ttttgattat
    30601 ctagcgatga atgcgccgga gcttgtactg tgaatgatga tagattgcaa atcgagagct
    30661 gggtgtcagg attaaaagtg cgaatggact cgatcttgtc tccgaaaaat tctattcgat
    30721 agggatgcat agattctgga ggataaaaat caataattcc acccttgatg cagtattcaa
    30781 atggactttc cactgctggc gctatggtat aaccgagatg aacaagatat tcgataaaag
    30841 cgtcgcgatt gactatttcg ttgacgtgat aggtgcgttt aagcttattg aaatcatcag
    30901 aaagcgggaa tttactttta agcgaagata aagtagctac aatgatcgta ttttgtccgg
    30961 tggataattt ttgaagggta tcgttagtga agtaagcatt gagcgattcc gtatcagaaa
    31021 ataggaagga agaattcggc ggctccggca gatagcatac cgagtcgtcg agattaaaca
    31081 gctccagatc atctcttatg tattccgctt cattggaatc attggtaatt aataaaattg
    31141 gctggtcaat ttttttataa aaagaataga gaaaaagcga agttgcaaaa ccggataatc
    31201 ccgcaacggt aaacccagcg ttagagcgat aaaaagcctt aacggcatta agaaacgctg
    31261 aactttcgga aaaggtagag actaaatttt ctatacgatc gtctatatgt ttcacgttaa
    31321 accagaatgc ttatgaagga ggaaaaaaag cgccgcgacg tctgccggag agacacccgg
    31381 aattcgagaa gcctgtccaa gtgtttgcgg gcggaagcga atcagctttt cacgcgcctc
    31441 attagttaaa gatcgaaccg cagaataatc aaaatcaggg ggaatcttag tgttttcgtt
    31501 ttttgacatt gattcaacta gagcttggct atggttgata taaccggcat atttgacgtc
    31561 acactcaatc tcctcaataa tcttgggaaa gtgagcaatc ttatttttta tttcagatga
    31621 taaaaaagga acgagcgaag cgagcgcaac atccggacgg caaagcaatt tatataaagt
    31681 gagattacag ccaatggaat gagcttcctt attaggtaat atgactttag ccgcttcggg
    31741 agaaatattt gttgaagata aaaaggatat aatctcctca ataacttcta tcttatgttg
    31801 ggttgattcg taagtctctc gcggcaatag accgagttca tatccttttc gggcaagtcg
    31861 gcgatcggca ttatcaaaac gtagcatcaa gcggtgctcg gcgctcgagg tgaacatacg
    31921 gtagggctca ttgggacttt tggtaattaa atcatcaatg agaaccccga tataggcttc
    31981 gtgcctttgc aaaacaaatg gttcgcgacc ttggatggaa agggcggcgt tgatactggc
    32041 cattaatccc agagcggctg cttcttcgta gccggaagtg ccattaattt gacccgctat
    32101 ataaagtcct ttgagtgact tggtttcgag agtatcttta atttggtaag aagggaaaaa
    32161 gtcgtactca acggcatagc ccggcttgat aaactccgct ttttcgagac ccggcactgt
    32221 tcttaatgcc ttcctttgaa cgtcaagtgg caggctggtc gaaaaaccgt ttacgtaata
    32281 ttcgttgcta tttttccact ccggttctaa aaatatttgg tgagacgtgc gctctttaaa
    32341 gcgaacacac ttgtcctcta tgctgggaca ataacgcgga ccaattcctt taattatgcc
    32401 agaataaagc ggtgaacgat ccagattgtt caaaatgaaa ttgtgggtaa cggtgtttgt
    32461 gcgggttaaa aaacaaggaa tattaggtgg cgagaaattt tgggtgctaa aagaaaacgg
    32521 aatcggatct tgatcgccat attggggcat tgtcctagaa aaatctatag attcggccga
    32581 gaccctgggt ggtgtaccag tcttaagacg tccagaaagg atattatatc tggcaaggtc
    32641 ttctgtaagg ccggagaccg gttcttcatc aattcgaccg ccatggaatt gttgtagtcc
    32701 gacgtggatt aatccattga ggaatgtgcc gcaagttaaa attacagcct gacaaaatat
    32761 gtcgccgtta tgaattaaat gaatgccttt aatggtctgc ttatcaacta tcaagctatt
    32821 ggcagtatct tcgagaaggg tgagccggtc ttgatttgat aaagtgttcc tcgccaacaa
    32881 cgagtagtga attttatcgg cttgagcgcg cggcgaccaa accgctcttc ccttagactt
    32941 gtttagcatt ttgaattgga ttccagtccg gtcaatcaat tttgccattt cgccgccgag
    33001 agcatcaatt tcagcgacga ggtgaccttt tgctaaacct ccgatcgatg gattacacga
    33061 cattctacca atggtagaaa tattttgggt gaccagtaaa gtagaaagtc ccaaacgagc
    33121 tgccgccaat gccgcttcta ttccggcgtg tccgccaccc acaacagtga tgtcaaagtc
    33181 taaatgtttc atgtgaaact actatttgcc gatgcaaaaa tgctggaaaa tattgttcaa
    33241 tatttcatca ttagaggttt ttcccagaat tttgtcatat tcattgagcg ccatccgtaa
    33301 atcggcgatg atcatctccg gagctagact ttgcctaatg tgtttctggg cagaaaacaa
    33361 atattttttt gccgtcctca gaccggtagc atgacgctga ttggtgatga tgaggctgtc
    33421 ggcgttgcta atttgttgcg agattgaatc aacgatcatc tcggttagtt tgtggattcc
    33481 ctggcgtttc tttgccgata taaatatatg gtgatattga gagaaatgct tttcatactt
    33541 tgtcaggctt ttttgggggg cgatatcaat tttattgatc acaacgatag tatttttaga
    33601 attgtaatac tctaaccatt ctcttttaat cggttgagtg gcatcaataa cgaataagat
    33661 aatatcgcct gtcttgatat ttttcttagt gcggtcaatg cctatttttt ctattgggtt
    33721 tttggttttg cggataccgg ccgtgtcaag tagtctaatc atatagccac cggcttggat
    33781 tgcttcttcg agtgaatctc tagtcgttcc cggataaggc gtaacgattg cgcgttcttc
    33841 gtttaaaaag gcatttaaca agctcgactt tccgctatta ggcggcccaa taattggcac
    33901 caaggcgcct tgctttagta ttttaccgca agaataggat tttagtagtt tgctgttaat
    33961 tttcaatatg ttatcaattg atttcgacaa ttcgtgcggt ggaatcgtat ctatttcatg
    34021 atctgaaaag tctaattcta attcaagaat agttattagc tcgatgattt tactccgaag
    34081 ttcagcgaat tcctttccga ttgtcccttc cagtgatttg atcgcggctt ggtgagacgc
    34141 ttgggtttga gagaaaacta aatcggctac ggcttcggct tgagtcagat cgaacttacc
    34201 gttgagaaac gcgcgtcggg tgaattctcc gggttgagcc aatctggcac catacctgag
    34261 gcataattca atcagttggt tgacaatgaa agggcttccg tgacagctga tttcgactaa
    34321 atcttcgccg gtataggaca ttggggcttt ataataaatt ataactgtgt catcaagcaa
    34381 ctcgttgttt ttgtataaat tgagatgctg agcaactcgt ggttttagtt gggtggtggt
    34441 tttggttaat ttatgaacaa tatccggtga ttttggaccg gaaagacgta ccactgccag
    34501 cgctccacgc ccgggaggag ttgaaagggc aactattgta ttttcggttg acattattct
    34561 cagtagaatt cattcagcga ccgcgcatcc ttgtttggta ttggcgtagt ctttgagaat
    34621 tggttttacg ggatttcttg ggttttggtt tgggtggaaa atatttttgc tgaataatcg
    34681 aaagaatatt ataaagagta tagtacaaat ttaaaccgga cggaaactga ttaaataaaa
    34741 agaaaaagaa gatagtcata aagtacatca tcactttctg ctgtttgccg ccagaagcac
    34801 cggatatttt ttgctgaacc aactgcgaaa ttgccataat aaaaggcaaa acatttacct
    34861 gactaccata aataggaatt gaaaatggta aggtgaaaat tgtgtccggt gcggataaat
    34921 cggtgatcca aaaaataaag ggtgcctgcc gcaattctat cgtagtgcga aaaactatga
    34981 aaagcgccca tagaatcggc atctgaagca gaagtggcaa acagcctccc atgggattga
    35041 caccatgctc gcgcatcaac ttaaggcgtt cattattaag gcgttgagga tcggaagcgt
    35101 atttttcctg aagagcgttt aactgcggtt gaatattttg catttctttc attgagcgag
    35161 tggaactgtt tgttaaaggg gtgaggacga tttttaacaa aacagaaaaa atgattaata
    35221 cccagccata gttaggaata aaaagatgca tcttggtgaa cagccataag attcctttac
    35281 tgataggcct aattaaacta aacccaaagt tcatcaagtg ttcgaaatta ggagccaagc
    35341 ctttaattat gctatagtca agtggtccca gataaatctt taccgcattt tctgaggaag
    35401 cgttcaaagt cagatacata ttgaatattt tttggaagtc tttgccttgt aatggaaccc
    35461 ctaagccagt taggcgataa ccttcagcgg gctgatgagg tatcaaggcg gcgacaaaat
    35521 acttggtttt tatagccgtc caataggttg cacccaccaa gctagctgat ccagttttac
    35581 ccggttttac atcgagattt tcggtttcgc cacccccgca ggcggaagcc cgtgaatagg
    35641 agacgtcttc tttgatcata gattcagtat aggctattcc gccttcccag accaggtcat
    35701 atttatcaga agccatttca tcgaggattc cctcgagatc attgtatgag tttataatgt
    35761 atttatcacc gtaaaagaca aaggttctcg aaacgataat atcatcgccc gctggcaatt
    35821 gaaactgaat aataatacta tcgccatcgg aaattttata atagttttta ctggtttctt
    35881 gatgttcggt aaaaattcta aaattttgat caatggtcac cgaatcccca ttacttttaa
    35941 tgtatttaat aaaaagccgc cttgcctcaa aaggcggaat catttcgacc agacttgtat
    36001 cgcgtccgat aattttattg tagtttttta agacgacgct ggtgatagtg ccgcctccac
    36061 gcgaggatat tttaagacga taaagatcat tttcgattgt gagcgtgtct ggtttttcag
    36121 ctcccaaaga agccgggcgt tgcacttcgc ttgagggatg ggattcggat aatttcgctg
    36181 gaatcgtttc agaaataacg ccttctggtg ttttttctat gactttttct tgagtgacct
    36241 cgggtgaatt tttcgaaatt attttaaaat agtagggcat tgtcaaaatt atgacaaata
    36301 tgagaacaaa gccgattagt gattttctat ccatttttat tcctttggag ttgggagggg
    36361 atcataacct cctgtatgat aaggatggca tttaagtatt ctggtgagcg aaagccaaga
    36421 agccttaaaa aagttgaatt tcttaaaggc ttctaaagca tattgagaac aagttggcgt
    36481 aaaacggcaa gtgttgggga aaaggggtga gagaagcaag cgatatgttt ttatgattaa
    36541 aataatcaag gtgttaaata ttttcattcc gtttataaat ttaggttgtt tatcagaatt
    36601 tctgataatg tgtttacctg caaattatca ggttttttaa aaacagtaac ggcgattgcc
    36661 gccggtcgtt tacaataggg tttacagaga cggaaagcct ccctgatttt acgtttgtat
    36721 aaattccttt gaacggcatt cccgcattct ttttttatca cgattgataa tttccaatca
    36781 tcttggggta agatggaaat gtaaaggcaa tcatatttta gcggttttcc attatgaatg
    36841 atttgggcac gttgatccca tttggcgagc cgttcacgac ggggcaaatt aggccgatag
    36901 gaccttacgt cctttagctc gtcttctggc gatgactttt ctgccactat tggtgctcat
    36961 cctttttcta aaaccgtgat cgttttttct tttacggttg ctgggctgat aagttctttt
    37021 catatttcct cattgttaat taaaaacccg cctaatttaa cttttttgac cttattatca
    37081 atgttagaaa taaaatgtgg ggtttagctt tggagtaact atggagtgcc cctttggagt
    37141 gcaccgacca tgtcggtgca tgcatcaaca cggttgatgc actccatatc actccatagg
    37201 ggctccatat attaaaaatc atccttaacc ttaactttat cccacttata cttttttcgt
    37261 aattcttcca tctcttcctt ttcggctttt atcctttcgt aataactacc aaatctccaa
    37321 tcactggcgt cctcaacata tccgtgtttc ataggattat accagatata attcaatctc
    37381 gccaaatatg acttctcata agtaatgcag gtgtcccaat aattatacat gacaatttta
    37441 gcatctttag attcaggaac attcttctta atccataaag cgatgaatct atgtatatca
    37501 tttatcatta aagaaagcgt tgaggcatcc tccggcgcat tacccattaa atggtaatga
    37561 ttatccagaa ttacccaatc ctcaagcgtc caatgatagt ggtcaaatcc tttttttatg
    37621 gacgttagaa gccgttcctt tgcctcatct gatttcagcc agggtttctt accgtaggta
    37681 gcaccagtta tgatatattt ggacccagga caaaacagat gcggtgggtt gtgttcatat
    37741 attttatatt cattgtccat ttttttactt tggaatatag ctttggagtg cagtgaccgt
    37801 gtcactgcat gcaccgacat ggtcggtgca ctccatattg gtcactgcac tccatattca
    37861 ttccataatc accaatttct tgttccacgt tcttttcaat tctttcgatt caatggaaag
    37921 ctgataaata tatatcccag aacaaagatt tttcggctgc caattaacca catattcatt
    37981 cgctctttga aattcattga ccaaaacatc aacttcttgg cccaagagat taaaaatcga
    38041 taatctgact tgacaatctt gaggaatttg ataagaaata caagtcgaag aattaaaagg
    38101 attgggataa ttctggaaaa gattgaaagt tagcggaatt tgttcatttt caattcctac
    38161 tggattaatg gccacccttg aaagattctg ccaattactt aaagaattgt ggatatgagt
    38221 tacttcttga agcgaggaag gaaaagatgg aggggtccac atagtccaga tatctcgagt
    38281 ttgaactttt ataacgtatt cacccggttc gtcccaagta tgactgaaaa caaactcact
    38341 tccgtttggc tgaaaattag accaatcagt cgtatcacca tctccccaac taacccgaac
    38401 tgaaactgag tcagtttcag cgtcgctaac ttgaatggaa aaagtaagtg tatcgcccgc
    38461 ataaccagag tttgatccgc taacctgagc cgtcggcact gaattccagg aatagagctg
    38521 gcaggtttca agatagcaac cactccattt tccgccagtg accaatactc gtccatcttg
    38581 gagaagggtt gcttgatgat ttattcttcc tccccgtggt ccttcgtata gcattggcgc
    38641 ctcttcttgg aactttttag ttttaagatc atataattca gagggagtga tttctatggc
    38701 cctctctttg catccaccga tgattaatac cttattatta ggcagaagcg tgactgtggc
    38761 tcttaatcgg ccaccatctg ttttaagaga gtcagttaaa ctccactgac cagtttcttg
    38821 gtcataaatt tcagctgact taaaatattg tcctttccat gctcctccga ccaagagaaa
    38881 accagagggg attattgttc caacggcgca gttcctttct ataatagatt tgccagtata
    38941 agtccattct tctgtttctt ggtcatagat ctcagctgat ggtccctgag aaccactcgg
    39001 ataagaatga aaagataaaa ctaaaccatt agaaagcatt ccgcccaaga gatctgggtg
    39061 accctgctga cttggattag ttaatctcca aacttctgtt tctgggttat atatttcaca
    39121 ggttttcgtc gtaactccgc ctattgtgta cccattaatc accaagactt ctccctcagg
    39181 taaaaggact gtagcatgat tagggtgatc ccagtttcta gctatattta gcggaccagt
    39241 gaaagaccag gtctcatctt ctaaatcaaa aagctcacaa tcaggaacac atcctacatt
    39301 tggaacattt cctccgataa ccagcgtttt attgttcaaa agcttgacta agtcaaagtc
    39361 atatctagct atgtttagag aatcagtcag agaccaggtt tcagtttcta aatcataaat
    39421 ttcagaggaa gataaaagtc caggtgaacc gggaccaata ccgccaacaa ccaaaacctt
    39481 accatcagct aataaaatag cccgatgttc tgttctggct tgattgagat tcccagtcca
    39541 ttgccatgtt cccgctgaaa ttataaccgg gaataggata aagcaaaaca aaacaatcaa
    39601 tactatcttt ttcatctata cctccatttt ttggagtgca gttttggagt gcatcaatcc
    39661 cgcagcgaag cgtggcgggg ttggtgcatg cagtgacacg gtcactgcac tccaaagtca
    39721 ttccatcatt atcaattttt tcttcaatgt tcttttgaaa ttttccgatt caatacttag
    39781 ctgataaatg tatatcccag aaggaagatt tttcggttgc caattaacca catattcatt
    39841 cgctctttga aattcattga ccaaaacatc aacttcttgg cccaagagat taaaaatcga
    39901 taatctgact tgacaatctt gaggaatttg ataagaaata caagtcgaag aattaaaagg
    39961 attgggataa ttctggaaaa gattgaaagt tagcggaatt ttttcatcaa ttcctacccc
    40021 tactggatta ataaccaccc ttagaagctt ctgccaattg cttaaagaat tgtgaattcc
    40081 ctgttcactc caaatgtctt ttgcctgaac cttgattttg tattctccgg cttcagcaaa
    40141 cactttggaa aaacaaaatt cagtgccaga aacatcatat tcctgccaat cagttgtatc
    40201 gccatcagcc caatcaattc taatactgac actatcttga tttggatcac taacttgaat
    40261 aacaaattct aaagtatcgc ccaccaaacc agtgttttgt ccctgaatca attgaatttc
    40321 cggagaatca ttccaagaat agagttctac tgtgtatgag cctgtcgtgc caaaactacc
    40381 aatagccgcg actcggccat cagctaaggt taccagatta tgatgtgatc gagggtaatt
    40441 catatcagct actcgttgcc attctccttg ctcaacatta tatatttctg taacagggga
    40501 aagtagggtt gtatccgtcc gataaccgcc agaaacaatt atatctccat tcggcagttt
    40561 agccgaaggc attgccaccc ctccccggct taaagaatca gtttctatcc aggtctcggc
    40621 aattgggtca aaaatcaagc aggtggttag ccaatgattt ccaccacttg ttccggccat
    40681 tattagtcct ttttcttgac cattctcaat aatcaattga gaggcgccat tttcatattg
    40741 gggacaggga acgggaatag gagttgtcaa tgtccaagta cgagaaggga tatcaaataa
    40801 ttcagcggtg ttagaattat catcaccaac taccagaatt ttctgatgag acggaaaata
    40861 gacaagtgtg tgttcagttc tcccttcatt taattcatta gttaattccc agatttcagt
    40921 ttgtgggtca taaatttcgc agatttttcg ggaaccacca gcgctgaatc caccgaccac
    40981 taagacttta gttgtactat cagtatcttg gaaagcaatt gcagcatgac tatagtgggg
    41041 agaattgagt tggcctgttt cttgccaggt atttgttgtt gatgaatagc gaaaacaacg
    41101 gcgagtggga ggacgaggac cagatgccat tccaccaata gttaagtatt ctttatctcc
    41161 agctaagtct agttcaatta aatagtgctg gtataaaggt actggcaaag aatcagtaaa
    41221 aacagcttgc cctgtttcaa aatcaaatat ctcacaactg ctggttggct cagtactccc
    41281 taaggataga ccgccagtta ttaagacaga ttgactatca ggcaaaagaa ctgattgaaa
    41341 gttttcacgt cggccaagat ttaattgacc aacaattgtc cattcatcag ccattagcaa
    41401 agaatcaaac aagaaaaaaa caataattac ggatattttc aaagttttca ttatactctc
    41461 ctttccttca aagagcgatt caagttgatg ttatactata atttccaaaa aatcaataat
    41521 gaatttttgc tttcggtaat tttaattatt gttagccgct aaactatcgc ctatttagtg
    41581 tcgagaaaat atttattttt taatatgctt tattacaatg acttaccgaa gatggcgcga
    41641 caatgttttg ttttatttca aactttttct tgctaattag tcgcaagcga ttaccttatt
    41701 atgagagttg aaattgtgat gttgataact tgttaatatt gggtggtgct cttattgttt
    41761 ttaaattatc tgttattgaa ggtttaaatt tgagtggagc tcttaatttt gtgaatctta
    41821 gggtgattat tctcgtgggc tcgatagcag actcgtcgga atatctccca atataattta
    41881 caatatgagt gggggctgga tgctaaacgg tttatataaa tggtgtttaa atgccttgta
    41941 tttatgttga taacttctat ttaggattcg ttttttatgc ctaattcatt gatttgcagt
    42001 gatgtttgga atagttgctt ggaatatgtg cgtaaaaaga ttcctcagca gacctttgaa
    42061 acttggtttg ctccgatgaa agccatatcg cttgccgatg atactcttat tttacaggtt
    42121 ccaaataaat ttttttatga ttgggttgac aatcactatc gggaaatgat tcttaaggcg
    42181 cttaaggagg taaccggtaa cggtttcaat gtaagttata ctattttatt gggtgaggaa
    42241 ttggaacagc cagccggaac gctctcagtt tctaaacgag ggagcggtgg tactccggta
    42301 gctgagccgg attataaagc gggggcacgg gagcaaggca cccgcttaaa tgagcgttat
    42361 gttttcgata cgttcgttga gggcgcttct aaccaatttg cgcgagcggc ggcaatggcg
    42421 gtagccagtg cgccggggcg aacctctttt aatccgctgg taatttatgg cggtaccggt
    42481 ctggggaaaa cccatttact tcaagccatt ggtaatgaag ctcttaataa tggcagagcc
    42541 aagaaagtag tctatgtttc cagcgatacc ttcactttgg atttcattgc ggcaattcag
    42601 aacaacaaga cgactgaatt ttctcgatct tatcgcagta ttgatatgct tctcctcgac
    42661 gacatccaat tttttcaaaa taaagaacaa acgcaggaac agtttttcca catttttaat
    42721 gacttatatc aaaggcataa gcagattgta ttgacaactg acaagccgcc aatggagttg
    42781 cgcggtttgc aagaacgtct cttatcacgc tttcagtcca gcctaaccgt tgatattcag
    42841 ccgccagact tagaaaccag gattgctatt ttgcagaaaa aagctaatga tgacggattg
    42901 gacattccct atgaagtaat tgaatattta tcagccaata ttcgttcgaa tattagggag
    42961 ttggaagggg ctttgatacg acttctcgct tattcttcgt tattgcaagt ggatatagat
    43021 attaatctta ctcagcgggt gttgcgggag atcctgggaa ctcgtcactc atcggtgtta
    43081 tcaatagaga gtgttcaaag tacggtgagt actgtttata agataaatct ggattctatg
    43141 gttgggaagg ggcgtaccaa agaagtggct gaggcgcgta tgattgcaat gtatttaacg
    43201 cgagagttca ccgatctatc cctaaaaacc atcggtttat atttcggcgg tcgcgatcat
    43261 tcgacagtag tacacgcttg ccgctgggtg gaagagcgcc tacaaagcga ctcggttttt
    43321 gctcgtcaga ttaatgatct gaaagccaaa ttgtcccaaa acatttagta atttttataa
    43381 ccacagatgg cgatccagac tgcggtattg aatagcttcg ctgatgtgtt cgggtaaaat
    43441 tttttcggaa ttgcttaggt cggcgattgt tcgggatact ttaagaatcc gatcgtaagc
    43501 tctggcactc aatcccagct tattaatggc gttgcgcaag agatcttcac aggtttgatc
    43561 aatttgacaa aatcttcgga tcaagcgtga ttccatatgc gcattagcaa agacaccctt
    43621 gatcccggca aaacgtgcgg tttgttttag gcgggcggct tgaacacgtt cccggataat
    43681 ttcggaaggt tcgccgggcg ctttcgctga cagatcggaa aatttaacgg cgggaacttc
    43741 tatatgaatg tcaattcggt ctagtaatgg accggaaata cgcgccatat atttctggat
    43801 ttgttgggga gtacaggtgc attgatgatg aggatcggtg gcatagccgc aagggcaggg
    43861 attcatcgcg gctaccagca taaaggaagc cgggtaggtt aaagagagta gcgcccgcga
    43921 aatggtaaca taaccattct caagcggctg acgcattact tcgagaacgt tttttttaaa
    43981 ttcgggtaat tcatcgagga aaagaacacc gtgatgactt aaactgactt caccggggcg
    44041 ggggaattta ccaccgccga ccaatgccgc atcggaaatc gtgtggtggg gcgaccggaa
    44101 cggccgagtg gctacgatgc cctggccgga gggaaccaaa ccggctacgg aatgtatttt
    44161 ggtagtttcg agcgcttctt caagagttaa attgggcaaa atagtcggaa tacgtttggc
    44221 gagcatcgtt tttcccgaac caggcggacc tatcataatt atattgtgtc ccccagccgc
    44281 cgcgacttcg agggcacgct tgacattttc ctgaccctta acctcagaaa agtcaatctg
    44341 gtattggcga tgttcttcga agagcgcccg gacatcaacg tgagtcggtt ccggatgggt
    44401 tcggttttcc aggaagtcta tcacttcctg aagggatttt aagccgataa tttccagatc
    44461 gccagttata gccgcctcgc gggcattctg ttccggcaag acgatgccct tgattccgga
    44521 attacgtact gaaaccgaga ttggcaaagc gcccttaatt tcgcgaagca ttccatcaag
    44581 ggagagttcg cccaagaaaa tgtaatcgct aaccttatgt ctttctatca aatcagccgc
    44641 ggtgagaata ccgatggcaa tcggtagatc aaaggagctc ccttctttct ttatatcagc
    44701 tggggctaaa ttgaccacaa ttttattgct cgggacatag tagccgagat tttttatcgc
    44761 ggaccaaact ctttctttac tttcacggac ggcgccctct ggtaaaccaa ccgtaataaa
    44821 agccggtaat tgcgcattac tcagattaca ttcaacatcc acaatatagg catcaatgcc
    44881 taaaacagct gcactcttga cgagggaaaa tttggtcata agttactcct tccaaactaa
    44941 aaaattggta accggtcttg ataaaacggc taaccaagcg ctatcttatt ggtcgaaatt
    45001 taatttccag tcgcagttca ttacattttt acgttgaaat tgtttttcat attgagcaat
    45061 ttcgcgagcc attttttcac aaacattatc gtgataagtt ttagcggcaa ctataatggc
    45121 attggtaatg gctttggcgc gactacggcc gtgagccttg attactattt tgcgaaatcc
    45181 gagaagaggt gcgccaccgt attctgaata atcggcaacc tttttcaatt gtttaatgcc
    45241 actggaaagc gcaagcaaac cgagcatcca gataaaacgg tgtttaaaag catagcgtcc
    45301 gatgtgagaa atagtttcac caaaaccttc gatagtcttc ataacgatat tgcccacata
    45361 accctcggcg acgatgatat cagctatacc ttttaaaatt tcgttacctt cgatattacc
    45421 tatgaatttc atatcttgga ttgaattaag catattaaaa gcgtcggtga gagtatcgcc
    45481 acctttgtaa ggttctttgc cgatattaag tagtccaacg gtcggatttt ctataccttt
    45541 gacggcggaa gcatatattt ttcccattat agcgaactgt accagatcat cggctgtgca
    45601 atggacgtta gcgccgacgt cgagtaagag ggcaaatggg tcagaccttc cctgttcgtt
    45661 gcgagtagga taggctgagg caatggcagt tttgtgaacc ccctcgatgc gtggaatgta
    45721 cttgatcgct gaaagaatat aagcaccggt attgccggcc gagacgatac catgtgcttg
    45781 atccgcacca cagagttgcg aggcgattaa cattgaagcg cgcggttttt tggataggat
    45841 aaatttcggc ttgtcgtcgt tggtaatata ttggtcggta tgaacaattt cgagctgttg
    45901 aggattgtgt tcagtttgtt caagaattgg ttctatttta gattggtcac caactaaaat
    45961 gatttttatg ggagtctcaa ccgaagcgcg agcggcaccg agcactactt catccggcgc
    46021 aaaatcccct cccattgcat caactgcaat aacggcagaa ggttgtttat tatctaagtt
    46081 attcatttta tgttttttag gtgcttaatc atttccggga taattgcgaa aagattagcg
    46141 acaattccat aattagcaat tttaaatatc ggggcgtcgg gatcattgtt gatcgcgacg
    46201 atgtatttgg cggaagatat tccggcaata tgttgcattg cgccggaaat cccacaagca
    46261 atatatagat ctggtgagac ggttttaccg gtttgcccga cctgaaaagt ttgcgggacc
    46321 caaccggcat caacaacgga acggctggcg ccgacggccg caccgagagc gtcggctaat
    46381 tcttctatca agtgaaaatt ttccggagca ccgatgccac gaccaccagc gacaataatt
    46441 ttggcatcag tgagtgctgg tcgctttgaa gcaactggac gaatttcatt aattttaatt
    46501 ttgggagtaa cggcagatag ctgtgacgga gagttgatga tttctgctga gcgaatattt
    46561 tcctgcggcg ggatgatatt ggctcggatg gtgataaaca ccggtttttt tatcaatttt
    46621 aaatcagcga gagccttacc gccatacata gctcggcgga cgataggact attttctgag
    46681 tcgagattga cttgtaaaca atcaacggct atagaaccgc taaccgtttc acaagcaatt
    46741 gccgcgatgt ctcggatagc tggtcggtca ggtaggatga gaatgtcccc attttcttgc
    46801 agtaatatat tggctacgcc tgaagccgtt acttctggta tatatttatc gaagttttta
    46861 gtgaaggcaa ctcggtcagc tccgtagcga ccgcattcgt tttgcatagt actattctcc
    46921 tgagccggga caatagcagt gaggcttcca ttggctaaat cagccaattg gcgcccaagt
    46981 gtcaaacagc ccgcgctatt cttggagata tttttgtttt ctggttcaat ataggtaaat
    47041 attttcatca ggattccccg ttataaaact ttggcttctt cacgcaggcg ccggatcaat
    47101 tctgggactg catccacgtt ctggcctaga atcgtaccag ctggccgtgg tgcgggttct
    47161 gacagttgaa taatatcgga agccttttcg gttaagttaa catttttggt aataatctct
    47221 ttttttcgag agagcataat atcctttagc gagggataac gtggtttatt caagcccttt
    47281 tcggtagtca ataaacaagg cagggaactc tcgtaaatta ttcggccttc ttcggtgtct
    47341 ttagcggctg ttaatcggtc tgtaccaacc tgcagttcgc atattcctgt caggaaagga
    47401 tattcgagta gacaggctac caaaggtccg acgagaccat tgccggtgtc aatggaaagc
    47461 tttccgcaga atatcaagtc cggagagagt ggcttgattt ctgtggctaa tgccttagca
    47521 gttgctaagg ggtcactgag tgctttatcg gcggtgacca gatgaatcat acggtcggcg
    47581 cccagagcca gtacgcgctt taagctgata ttatcccgct cgggtccgag tgaaatggcg
    47641 attacctcgc cgccgttttt ttctttcaat agaacggcgg cttcaatagc aaattcatcg
    47701 taaggattca ggtcgaattt aatgttgaca agatcaatcg ctgtcttttc cggtgtaatg
    47761 gttatgattg aatcggatac cggaacaact ttgacgcaaa ctacgatttt cattactctt
    47821 actcctactg accactgacc atcatccgat caattttgaa tgtcggtgaa ttaattgagc
    47881 cgcggaattc cagatcgtta cctatcatac taattgattt taacatttct aatacattgc
    47941 cagctacggt gaattcttga accggatagg caagtttgcc attctcgatc cagatacctt
    48001 ctgcgccgcg cgaataatct ccggtaacgt aattaatacc aacccaattt aaatcggtca
    48061 gatacaagcc gttgctgaca gaaccaatta tttcttctgg tgaatatttc ccggctggca
    48121 gaaagaaatt cgaggttgac ggtgaggggt cagtgcttgt gccacggcgg gcgcttcccg
    48181 taggagtgca ttttaattta cgtgctgagt agcagtcgca taaataagtt tttagaatac
    48241 cattttcgat gacaatgttg cggcgggctg gcagaccttc atcatcgaag tagcgagaat
    48301 cgataccgcc cggcaaagta gcgtcatcaa taatatttag ttcctcaacg gcgataggct
    48361 gattgagttt atcaaccagg aaggaatttt tgcgataaat ggcactgccc aatacgacac
    48421 cagcaagaat agcaagtaga tcggcaccgg tttgaggatc ataaacaacc ggaacagatt
    48481 gggtggtcgg tttacgggct ccgattttgc ggatggtgcg cgcggccgcc gttttggcga
    48541 tggattcgat tgaatccagc tggctgaaag cccgctgacc cgaccaccag taatcggttt
    48601 gtttgacacc gtctttttcg gcgacgagtt gtaaagaaag tgaacagcta gaaaattcct
    48661 gcacgccgga aaaaccttgt gaattgatca gaatggattg accataagaa tcgttccaga
    48721 atgagccgcc cgagttggtg attagtggat cttgtttcag gccgagatct tccagctcgg
    48781 tagccatttt gatcttgcgc tcggtaggaa tttgagcgat ttcgggatcg taaagcttaa
    48841 gatcagcttg cactttacca aacaaatgct tctccggcaa tccattgtat tcgtcagtgc
    48901 tggaaacccg gacaatttcg agagctttct gtaccatttt ggaaattgat tcggggcgca
    48961 agtcactggt gtatgttaga gctttacgct tattcttgaa aacacggatg ccgagtgctt
    49021 taggattgga ctgtcgcaat tcctcgactt ttccgaggcg gattttgagc cgggtattgg
    49081 tgaaattatt tacgagcacg tcaacctcat cggcgccacc gcgacgcgcc tcggagataa
    49141 cattttcggc tattttgact aattcttgca tatttttcct cgtaattaag agccgcctac
    49201 ggtgatttca ctgattttga ctgtgggatt accgacgctg actgggatat tttgaccgtc
    49261 tttgccgcaa gaccaagtgc cgtcagaaat tttcaagtca ttgccgacca tcagaatttt
    49321 attcattgta tctgggccat ttccaatcaa ggtgacgtct ttcaacggtg ccgttaattt
    49381 gccgtcttca atcagatagg cttccgttac cccaaaagta aaatcgccct tgatagtatc
    49441 aacttgcccg ccgctgaagc ccttggcgta aacgcccttt ttaaccgagc taatgatttc
    49501 ttcggagtcg tagttgcctt tttgcaggta agttgtggtc atccgagcta ggggcgggta
    49561 ggcgtaagat tgtcgccgcc cattgccggt ggggttagac ttcatcatcc gggctgaaat
    49621 gctattatgc atataaccct tgaggatacc attttcaatc agaacggtgg tggtaggtaa
    49681 attgccttcg tcatcaacgt tgagggcgcc gcgtttgcca gggaagaggc ctgaatcaat
    49741 gatagtgcaa ttttcggcag ctactttctg accgactttt ccgctgtagt tagataggtt
    49801 tttatagcta taatcggctt ccagtccatg gccgacggct tcgtggatca gcacgccgct
    49861 ttcaccggcg ccgagaatta cgacctgcgg tccagctggt gccggtcgcg catctaaatt
    49921 ggtaatggcg gttgcggccg cttggcgggc gatagctgct gaagtatttt caggcttttc
    49981 gaaaaattcg agccccgctc gggcagagcg agaagcagat gcaccctggc gtcggacacc
    50041 atcttcagct ataacctgta ctctgagact cattaatggt tgaatgtctt gaaatttttt
    50101 accattggag tcaaagtaca taatttggcg cagttcctca gaaaatgaag ctattacgct
    50161 ggagacccgc gcattgaatt gtcgggcgat cttatcagct tcattgagca ttgtaatttt
    50221 gggattgaga tccatatcaa tactataggt cttaaccgga tataaatttt tagccgctac
    50281 tttctggaaa tgtgcgggtt taaaactctc cgtactattg gcaatattag cggcggtttc
    50341 agccactcgt ttcatacttt gaaagtcaat agcgtcggtg taggcataac cgatttgatc
    50401 gcctttaata gcgcggacac ccattcccag agaaatgcca tattgaatat ctttaatgat
    50461 tccatcctca atacgcaggc tggaattggg acggtattca aaaaatagat cggcgctttg
    50521 cgctccgcgg gaaagtgcca gccggagaag tttctcagct tcaaccgccg atatgttaaa
    50581 aagattttcc agatcttgcg tctcggatac ggttttagaa aaacctgcca tatcaaacca
    50641 cgctggagcc gtaacgattc cagccgctat cacaccactt tttttgataa attcgcgtct
    50701 cttcaagcca cctcctcaga tgggtaatat ttttttgttt atagttatta ctttggaaca
    50761 atttaatgtc aacttattaa ttcacattac cagattcaaa tataatattt gaggttaata
    50821 tacgaaaaaa agaatggttt taaaattctg gaataaaaat aacgcttgtt ttgtccaacg
    50881 aaggaaaaat aatttattat ttgttcaagt ttttaaaaat ttaccgatta acacacatac
    50941 agagtattat tgttcggaaa aaattaatat attcggtcgt taatttgaat tttaagagta
    51001 agtcgaatct attggaagtc agttaaatgg ttaagaaatc agttctcttc atctgcaccg
    51061 ggaattcggt gcggagtcaa atggcggaag ctcttctgcg tcagttaggc ggtgatcagt
    51121 ttgaggtttt cagcgccggt tccacgccgg tcggaattca ccgttttacc aaacaggtat
    51181 tagccgaaat cggtatctcg gttgacaatc agtattctaa acacattgat atgtatcgcg
    51241 attataactt cgactatgta attgttttat gtgaggttgc cgctttgaat tgcccgactt
    51301 ttcccggcca ggttgagcgc ctagaatggc tgattgatga tccgatccga gtcattggaa
    51361 atgaagagcg gcgtttaatg gcttttagga ttacccgtaa tattctcaag gaacggattg
    51421 agaaattcat taaggagaaa ggtaatccat ctacttaata attgttaata aacttgggaa
    51481 agccagttga ttaacttttg cccgatttaa tcttctaaag tttgttatga tagcctaact
    51541 tgacgagggt cgaaaaaatg attacttttg attcagattg gaacaacgct attgataatt
    51601 tgttgaaaat tctatgataa agaaacttaa atccattctt ttaatactga ttttcgcgac
    51661 ggccggattt tcacaatacg gtaaaaataa agttcagtac gatacattca attggtcata
    51721 tttgcaatcc gagaatatgg atatttattt ttatgaaggc ggagaatctt tggccgagtt
    51781 cgcggctccg gttggggaac aagctctgaa ggacatttgc aaagtgctga actggcgctt
    51841 gcgtaagcgg gtgactttaa tagtttataa ttcgcacagc gattttcaac agaccaatgt
    51901 aaccctttca tatttatacg aagggattgg cggatttact gagcttttca agaaccgcgc
    51961 cgtagtgcct tttgaaggct cgtatttcga tttctggcac gtgatccggc acgaactggt
    52021 gcacgtggtc atcaacgata tgatttatgg cggaaatgtc cagagtgtgg tctctggtcg
    52081 agtgagattg agtattcctg attggatgag tgaaggcttg gctgaatata ttgcctacgg
    52141 ctggacggct caaaccgatt tgataatgcg tgatattgct ctgaatagca atattcccaa
    52201 tatcgaggaa ctcgattatt actcggctta tcagggcggc aactcagttt atcgttttat
    52261 tgctgaaaaa tatggtatcg agaaaatcgg tcaaatctgg gctcaaatga aaagcaaggg
    52321 taatgccaat aacggtttga aagccgccat tggtatggat atgaaagagc tgactgacaa
    52381 gtggcatcgt tggatgcgca ctgaatattg gccggacgtt gctgaccggt atgatattga
    52441 agagatttct atgaaattga ccgatcataa gaaattgaaa aattatttca ataccgcacc
    52501 agccatttca cccctgggtg ataaaatcgc cataatgacc gatcggaacg ggtacgccga
    52561 tatttattta atttcggcgg ttgacggcaa ggtaattaag aagctgatca gcggtcagcg
    52621 gacaccggac ctcgaagaat tgaaatggct caatccgcgc ttgagctggt cgccggacgg
    52681 caaaaaagtg gttcttgcgg ctaagagcgg agggcatgat gccttaataa ttgccgacgt
    52741 agaaacccaa aaacgtcagc gctttgaatt taaagaattg gaagaaatat ttacgcccgt
    52801 ctggtcgcca aaaggcaatt cgatagcctt cgtgggattg aaaaatggcc aaaccgattt
    52861 gtatttatat gatattaaga aaaaatccct cacgcagctg ataaatgata aatatcccga
    52921 ctttgaaccg tcctggtcgc cggacggtca gagaatagta ttcgtttctc aacgaacccc
    52981 ggagatagac agcctgccca atttaccaga gattatgaat gtcaaatgtt ttcaaaccga
    53041 tctattttgt cttgatttag ataacggaga aattacgcgt ctaaccgatt cgccctggaa
    53101 tgaaaattac ccgtgctggg cgcataccaa ggacgctatt ttttatactt ccgattatca
    53161 cgggatttcc aatattttta tgttggatat atccaccggc gagagcggtg cgattaccaa
    53221 tgtattgacg ggtgtttttc agcccagtat ttcgaatgat gacctgcgtt tagtattggc
    53281 tggctatgcc gatgaaggct gggatattta ttcaataagt aatccattac aactttggga
    53341 cgaacgtcag gaaattaaac caacagttta cgctaaagct ttaatggaat cctgggaaaa
    53401 tccggagaaa taccaaagat tcaatttccc cagtaaagaa gaagccaaac cccgaacacg
    53461 acagaatata attgccgccg gggatttctc taattatata tttgcaccga tgtataatta
    53521 tgagatcagt gcagagcctg aagaagattt tctggattca actatggcga gcgaggatac
    53581 taccaaacaa accaccaaaa gtggcagata cccggtcttt ccgtacaaaa ccaacttttc
    53641 gctggatgtg gcttatggcg aggctggata tagtaacctc tggggcttgc aaggcactac
    53701 ggttttttat ttcagtgatg ttttaggtaa tcatcagctg gcattaggaa ccgagatgta
    53761 tatagatctg gaaaacagtg attattattt atcatatatg tatcttgcca agcgtaccaa
    53821 ctggggcata accggatttc atacgtcgaa cttttggaat tatggatttt ttacggtatt
    53881 tcgtttaaga aattatggac ttgatttttc cgcatcgcgt ccttcgagtc acttttcgcg
    53941 gactgagcta gggctaactt cctataatgt cgagcagaaa tatatcctga ctctaaccgg
    54001 agaagagtta tacacatata gaattaacac aattttgcca cgggtcggtt ttgtttatga
    54061 taattcacta tggggttatc tatatccgat tgacggttgg cgggcgcggg tggattttat
    54121 tgccagcccg aagtacagcg ccaaaagtct ggagttttat agtgttcaag ctgatattcg
    54181 tcgttattta aaattaaaca tggattattc tttcggtttg cgcctctcga ccggtttcag
    54241 taatggtaaa aatgctcagc gtttctttct cggtggcgag gataattgga taaattatta
    54301 ttaccgttat gatattttca ccgctgactc gacgcttgct gagaacatct atttttcaga
    54361 ttttattacg ccattgagag gggctctgta ttatgaaaga gccggtaatc gctattttct
    54421 gtttaatctg gagttccgct acccgtttat taggcaactt gtgttaggct ggccgctacc
    54481 ggtaacgatg ggtgggattc aaggcgtaac ttttttagat ttcggttcga cctggtatga
    54541 taataaattt catcccttca agaatgatta ctatcgtgga tttatgatgg atgacctggt
    54601 agctggatat ggtatgggag cgcgcattta tttgggagtt tttgtattaa agattgacgt
    54661 cgcctggcgg tttgatttcg accggactta tagtccgagg tggtatttct gtctcgacac
    54721 tgacttctaa ttcaattctt cgatttactt ttaaaaatgt ttggcgttaa ggctggcgct
    54781 aattagttta gcgggtgatt aattatcccg aatgaatatg aaatatttca aattgatact
    54841 tatctgtttt atctggagca ctacttgggc ggtaatcaag attggtctcg atcgaacccc
    54901 ccttatggtc ggactggcgt tgcgtttcag cgtggcgaca atcgcgctgg gtattattat
    54961 ttttctgaaa cgccgtaaga ttgcctttga ccgtgtatcg gtacagaatt acttggtagt
    55021 tggtcttttg actatggcgc ttagttttta ctgcacttat tgggcggaaa aatttatccc
    55081 gagcgggttg acttcaatct tatggacagc tatgccgatt gttgtgggga tatttgctca
    55141 ttttatgtta ccagatgaac gtttgcgctt agcccaggtg atggcgattt taatagctat
    55201 ggctggtgtg atagttatcc tttccgatcg ccagctggtt tttggtcgag aattattatg
    55261 gggtagtgtg gttgccttat ttggggtagt cgtatcttcc tggccgaatg ttttactcaa
    55321 gacgcgtcct caaccttatg atagtttgac gatgactgct atggcaatgt tgattgccgc
    55381 acttgtgaat tttataggag caacggtcgg cggtgaatgg cagaaaatgg tctgggactt
    55441 caaaaatatc ggggcgatcg tttatcttgg tctgttcggt tccgcgctcg cattttatat
    55501 ttactttgat cttttgaaac aaattaatgt gatcaagctg acttttatta atttcatttc
    55561 gccgatattt gcgaccttga tcggagcatt gtggttgaat gaaatcgtta cctggcgtga
    55621 aattggtggg atgctcatta ttttcttcgg actatttttg tacgacaagg ataaatactt
    55681 agccatattg aaaataaaaa acggaaggag tacccatgcc cgttaaagtc aaaaatgtct
    55741 ttgtatgcaa tgagtgcggt tcagttcagc ctaaatggct gggcaaatgt ccggaatgtg
    55801 gcgcctggaa ttcgatggtt gctgaacaaa ttcagaagaa accgaggagc tccgctgtcg
    55861 agagaactcg gcgtaatttg gtgcctctta atcaggcaag taccacctcg acccagcggt
    55921 tggttaccgg cataaccgag tttgatcgcg tgttgggcgg cggattcgtc agtggttcgg
    55981 tagtattaat cggcggtgag cccggcattg gcaaatcaac tctaattatg caagtcatcg
    56041 cccgactgcc gggggaccgt ttactctatt tctctggcga agagtccgaa gagcaattgg
    56101 ctttgcgcgc tcggcgactg gaaattaatg atgaccgtat tttggtagcc tgtgagaata
    56161 atctggaaaa tattttagat caaatagaga acctgaagcc gaccgcggtg gtaattgatt
    56221 cgattcaaac cgtttactcc gatcagtttg aaaatgtccc gggcagtgta actcaggtgc
    56281 gcgagtgcgc cgggcgactt ctgcatcttt ccaaggagtt aaatataacc actgtttttg
    56341 tcggtcatat taccaaagat ggtatgattg ccggaccgaa ggttttggag catctggtag
    56401 atacggtttt atatctggaa ggcaataaga ataattttta tcgcaccttg cgggcggtca
    56461 aaaatcgctt tggttcgacc aatgaagtcg gaatattcga aatgaaggag cgtgggctaa
    56521 tagccgttga cgacccagcc ggtttctttt taacggagcg tcgtgaatac gttgctggct
    56581 cggcggtcac ggtcagtatg gaaggcacgc gcccttttct gatagaggct caggcattgg
    56641 tgacgcagtc gtcttacggc acaccgcagc gttcggcaaa cgggattgat taccggcgct
    56701 ttgtgatgtt ggtagctgtt ctggagaaac gcgctggttt gcctttgcgc actcaggatg
    56761 ttttcattaa tctggcaggt ggttttaaaa ttgatgagac gggaattgac ctgggtgtga
    56821 cggtggcggt agcgtcgagc tttaaagatg tcccactcga accgaaagcc gctttcatcg
    56881 gcgaagttgg attagttggt gaagtccgca gtgttccatt tttagaaaat agggtacgcg
    56941 aggcgctcaa gtttggtttt accacggtct atgtacccaa gtcggggata aaaattttag
    57001 aaaaaagcct gcaatccaaa gtaaccggca tcgaatcgct aaatcaacta ttcggattta
    57061 ttttctaagt tcggaagatt gatatgtcaa atcacggccg aaaggaatct aaagagaaaa
    57121 atcaactgcc gtttgatatt attgcccaag ataacggtac caaagcccgg gcagggatat
    57181 tcgtcactga tcacgggatg gtcgaaacgc cggtttttat gccggttgga acaagcggag
    57241 tagtcaagac tctgacgccg actgagcttc aggagtgccg cgttcaaatt atactaggca
    57301 atacttatca cctttacttg cgaccgggac tcgatgtaat tcaggcggct ggcggtctgc
    57361 atcgcttcaa tgcctggccg gggccaatat tgactgacag cggcggttat caggtgttca
    57421 gcctggctca tctcggtaaa ttgactgagg acgagattgt attccgctcc catattgacg
    57481 gttcggagca ccttctatct ccagcaattt ccatggagat tcaacgaacg ctcggcgctg
    57541 acattgtgat ggcattcgac gagtgcacac cctatccctg cgaccgagaa tatgccagac
    57601 aagctctgga gcgtacccat cgctgggaac gactctcgcg ggagcatttt ctgaaattaa
    57661 aaccgctcta tggtcatcgt caatatcagt ttggaatcgt tcagggctcg gtatatcccg
    57721 atttgcgggc ggagagcgta aaggccttga ccgaagtgga tttcgacggt tatgcagttg
    57781 gtgggttggc ggtcggtgaa cctaaagaac agatgtttga aatattatct ttggttgcac
    57841 cacaattgcc ggaaaaaaag ccgcgttatt tgatgggagt cggcaaaccg gaagatattg
    57901 tacgcgctat tggctgggga attgatatgt ttgactgtgt tttgccgtcg cgcaatgccc
    57961 gcaacgggac actttatact tggaaagggc gcatagtatt gaaacagagt caatatcgaa
    58021 tggacttcgc gtcacctgat ccgaactgta gttgttatac ttgccgcaat ttttcacgag
    58081 cttttctccg ccatcttttt atgaatggcg aaatgaccgg attgcgcttg aatactcttc
    58141 acaatgtcca cttttttatg gagctggtca gtcgtgcccg tcaggcgatt ttaagcggtg
    58201 actatgagaa ttggcagacc caattttttc gtaattaccc cgaagaggcc gaccattcgc
    58261 tcgaaaacga ggcgcgccgc tccgaacgtc gccgtaaaca tcttgaagaa aatgacataa
    58321 atagtgtgta acttatgcgt caatttttga aaaagtcatt attagaaagt atctgcaata
    58381 tagatgaacc ttaatgtttc ctatcccccg cccgatctgg cacgtttatc tcaggaagaa
    58441 attgagcggc gcattggcaa aatccgggct caatttggtc cccaactaat cattctggcg
    58501 catcattatc aatgtgacca gattgttgcc caagccgatg ccgtcggcga ttcgtttgcc
    58561 ctgtcgcgtc gagcatctga aacgaaggca aagtttatcg tattttgcgg ggtacatttt
    58621 atggcggaga cagctgattt attgaaaagc gccgaacaaa aggtgttttt accaaatgta
    58681 gatgccagtt gtccactggc agagatggct aatatagaaa atgttacaac cgcctgggga
    58741 aaaattcagg cgcttaccgg ggatcagttg attcccgtaa cctacattaa ctcgcggctg
    58801 gagctgaaag ccttttgtgg caaaaatggc ggtatcgttt gcacctcctc caatgcgcaa
    58861 gccgctctcg aatgggcttg gtcaaaaaag ccaaaggtgt tcttctttcc ggatcagcat
    58921 ctcggtcgca atatagcttt aaaaatgggg atctcgccgc aggaaatcgt tatatatgat
    58981 ccgagacgac caattgatga taaatttgca tggcaaatta aacaagcccg cctgataatc
    59041 tggagtgggt tttgcgaggt gcatcaggct ttttcaagaa atgacataga aaggctacgc
    59101 caggctattc cgggtattaa gattatcgtt catccggagt gtccggaaga ggtagcgttg
    59161 aatgctgatt ttgtagggag taccgaatat attattaaga ctgttacggc aggggcgccg
    59221 ggcagcgcct ggggagttgg taccgagcga aatttggttg atcgtctggc gcgccagttg
    59281 tcagaccgta aggtgatttc attgcaagaa aaaggtgcat attgctcgac aatggctttg
    59341 gtaacaccta tccacttgct ttatcaattg gaatgtctga gagatggccg cctggtcaat
    59401 caagtggtag cagatgataa tttgagaacc gatgcgcttc ttgctgtaca aagaatgatg
    59461 gagttgtagc tttgaaagcc gctgaacctt atcgcatgac tgatgttttg gtgattggtg
    59521 gaggcttagc cggttgttcg gcggcttatg ccgcggctca agccggattg cgagttgtat
    59581 tattgactat caatcaggat gccagtgtct catcgtctta ttatgcgcaa ggtggtatta
    59641 tctataaaaa tccagccgac acgctggcag attacatagc agatttccag gaatcgggag
    59701 cgcaattagt aaatgaaaaa gtagtcgaag caatttacac caatggaccg cggctgatag
    59761 aacagattct tattggtgaa ttgggggtgc cgtttgatcg gacgccgcag ggtgatattg
    59821 ccctaactcg cgagggagtt cattctaggc caagaattgc ttttgtcaag gattataccg
    59881 gtcgggcgat ccaggaacgt tttcacaaga aaatcagaca ggtcgaaaat attgaagtat
    59941 tgcctaatac tacttgtgtg gatttgataa ccctggcgca tcattcttta aatccgcttc
    60001 atatttatga gccaagcacc tgcgccgggg cttatgtata ttttcaagac agtggtgaga
    60061 ttcatccaat tcttgccaag caaaccatat tggcttcggg tggattgggg cggttgtatt
    60121 tgcatacatc gaatccctat catgctcgcg gtgacggtta tgctatggcg tatcgggcag
    60181 gcgcacgtct aatgaatatg gaatatgtcc aatttcatcc gaccacgctg ttttcaacgg
    60241 aagatgaacg ctttttaatt agcgaggcgg tgcggggcga gggtggtatt ttacggaata
    60301 aagatggcga agatttcatg ccgcgctacc atccgctggg ttcattagcg ccgcgtgatg
    60361 tcgttgcccg aagcgttatg caggaaatca gtcgcaccaa ggctcatttt gtctatctgg
    60421 acattactca taagagcgcc gattggctga agcagcgttt tccgcagatt tacgaaaaat
    60481 gtctatcggt gaagattgat atggcaaccg aatggattcc cgtggtgccc gccgctcact
    60541 ttgaatgtgg cggagtagcg gtggatcttc tgggcaacac tactattcgg cgtttaaaag
    60601 ctgtcggtga agtggcttgc actggcttac atggcgctaa tcgtctggca agcagctctc
    60661 ttctggagtg tttagttttt gggacactgg cgggacgcga ttgtgccgaa cgaatcggga
    60721 gtgtggagat cattactcct caagtagccc cttggcaaaa agaaaacgag ccgctcgacc
    60781 cggatcttat tcaccaggat cgtctgacta tcagacatac catgtggaat tatgttggta
    60841 ttgtccgaaa tgccaagaag ctagaccgag ccggtggaat tttatggcgc ttacaacaag
    60901 atatcgaaaa attttacgcc catagtcaat tgaatgatga gttgatcggc ttgcgcaatg
    60961 ccgctacgac gacattgctg gttttacatg ccgctcgcct gaataaattc agtcgcggtt
    61021 gccattatcg tgtagattaa ttaaaattgt aataaaaatg gaggttatat gaatagtctg
    61081 ttatatgctg gagcaacagg tggtggtagc ggagcccagc cagccaatcc catcctttca
    61141 ttgttgccgt ttttgctgat cattttgtta atgtattttt tgatgattcg cccgcaggct
    61201 aaaaaacagc gggaaaaagc caaaatgttg caggaaattc agccggggga cgaaattgtt
    61261 actatcggcg gaatttatgg caaagtcgaa ggcgtaaagg ataaaaatat cctgattgtg
    61321 agagtagcta aagatgtcaa aattaacata tctcgttcgg cagttgccag taaggtagag
    61381 gaaaaagtgg tttcgtaaaa gatggcggta cttccgattt acatttatgg tagtccgatt
    61441 ctacataaga aattggctcc gattgatgat ttgccgccag atttcgaccg cattctttcc
    61501 gatatgtggg agacgatgta tgatgatgaa ggaatcggac tctcggctaa tcaggtgggg
    61561 cttgatctac gatttttcgt agcggatttt tctttgcggg aagagaattt agggcgtgag
    61621 gtcataatta atccggtgat aatcaaagtg gaaggtgagg tatccagcga ggaaggttgt
    61681 ctgagtctgc ctaatattcg agaacagata ccgcgggctg agaaaattat cctgcgttat
    61741 gaaaaccgag agcgcaaggt tatcgagcgc gaatttaatg atttggtagc gcgcgtaatt
    61801 caacatgaga cagaccatct tgacggatta actatggttg accgcatcag tcctctcaag
    61861 cgcagttttt taaggtcgaa gctgaagcgc ctggcgacgg ccgccgctga aggcaagttc
    61921 gccaaagata ttgagtgagt tcagatgaaa atcattttca tgggcacgcc ggaatttgcc
    61981 gtgccgtcct tgagaataat agccgactcg cggcatgaga ttaaggcggt agttaccaag
    62041 ccggatatgc cttgtggccg tggatgtcgc cttcaggaac cgccggtaaa aaaagttgca
    62101 cgccaattaa acttgccgat cttacagccg gactcgctta agtcgccgga ttttttagct
    62161 gaaatggcac aattttcggc tgaactttat gtagtagttg cctttcggat tttgccaccc
    62221 gaacttctgg taattccacc acgcggcgcg attaatttac atgcttccct cttgccacgc
    62281 tatcgtggcg cggcaccgat caattgggcg ctcatcaacg gtgaaaaaga aaccggcgtg
    62341 acggtgttcc aaataaagcc gaccgttgat accggtaata tcttattgca ggaaagaatt
    62401 gcgattcagc ctactgatac ttatggaacg ctggcgccgc gtttagcgga aataggtgcg
    62461 cgcctgatcc tggcggctat agatggtctg gaagccggaa ctctaaaagg cacacctcag
    62521 gatgatagtt tggctacacc tgcccctaaa attttccctg aacttggaga aattgattgg
    62581 tacaagccag ctagcgattt aaaaaacctg attcatggat tatcgcctac tcccggagct
    62641 tttacctttt tccggggcaa aagattgaaa attttgcgcg cttctgtcat agatgaaaaa
    62701 attccgggtg aaccgggaac gattattcaa tgtaataaaa acagcctgaa cgtacttacc
    62761 ggtagtggag ttttaaagct ggaagagttg caatttgaag ggcgcaaacc tttacctgcc
    62821 gctgaatttt tatgcggttt tggaggacga gtgggagata aatttgcctc gtgaatactc
    62881 gtgccgccgc tgttatgatt ttgaatactt tgccagccac cggctataac tacgagtcag
    62941 ccattgacgc ctttctcaag attaataact gcagctcacc ggacagcgat ttcatctatc
    63001 tgttggtaaa aggcactatc caaaatcaaa gcttgctgga ttatttaatc gagcaaacac
    63061 ggaacaattc agtcaaacgc cttgagaaaa tagctcgcaa tctattgcga gtcggagttc
    63121 tgcagattgt ggttttgaaa acgccgcccc acgcggcggt gaatgagacc gtagcggccg
    63181 cccatcagct ccagcgctcg gacttggccg ccttgattaa tggtgtttta cgacatatac
    63241 ctcaggaatc agtctggcgt gagcgtctgg ggcaattaga attggcggaa gcgctggcaa
    63301 tcgaattttc ccatcctcgc tggttagtag agaaatggct gaacgctttc ggtcgtgaga
    63361 atacgataga tttgcttgca tttaataata aatatcaagc gatttttttt cggcacaacc
    63421 cactgcgcat tcattggagc gaatttgagg caaaactaca ggctgataac taccggattg
    63481 agatatatga aaccgcaccg gtagtatttt ttctagttga ccgaccgggt gacttactta
    63541 aaagtgaata tttcgagtgc ggttactgtt cggttcagga ttatagtcaa tcgctggcgg
    63601 tgcgcctttt ggatccgcaa cctggcgaaa aaatattgga tgcttgtgcg gcgcccggcg
    63661 gtaaggcgac tatgatagca caattgacca aaaaccgagc caatatcctg gcaaacgatc
    63721 gcacgattaa taaaattgag tcaatcaaag aatccgcctg ccgattaggg attgattcct
    63781 taaattacag cgtgaatgat gtcgccgcag cgaaatttcc tatgtttgat aaaattctat
    63841 tagatgcacc gtgtagtggt agcggcgttc tggcgcggcg ggctgacttg cgctggagtc
    63901 gtaaaccggg ggatttgctt caggcgcaag ccttgcaatt acggctactc gaaaatttag
    63961 caggatttct gcgtgataaa ggcgttttag tttatagcac ttgttccatt gagccagaag
    64021 agaattggga aacggtgcaa atgtttctag agcgctatcc gcatttttca gtggatacgg
    64081 ctgtaaaata cgttgatcaa cgctactgtg atcaaaaggg ggcagttatg gtcttgccat
    64141 ttgaacacaa tctgaccgga agctttgcag tccgtttggt aaaacattaa aatagaagta
    64201 gttaggagca aatgtcaaca aagattcgta actgtttgag attcatagcg gcttttatag
    64261 cagtcggcgt ggtgctggtg ctagtttttg ataaaatatt gatgcctatc tatgttcgtc
    64321 atggaaagga agtcgcaatg attgatgtgc tcaagctacc ggccgataaa gctatccaga
    64381 tattagatgc caatggtttt gaagtccagg tcgtagatac tattgagagt ggtaaccttc
    64441 ccatagggat tgttattgat caacagccgc ctcctggacg gatggtcaag aaagggcgag
    64501 ttgtacaatt agtagtcact ggtggtagcc gttttgtgcg tatgcccaat ttggtcggcc
    64561 gtgctctgaa agccgctcaa atcatcttgg aaaataataa gctcgttctc gattcggttg
    64621 aatatctcta ttcctcagag attcctgaag gagtggtcag cgaacaatct attctgccgg
    64681 gttcaatggt tagcattaac actaaagtca cagttgtggt cagtaaaggc ccgccaagcc
    64741 gccagcttga agtgcccatg ctaattgggc tcagcctgac tgaagcccga gctagtatcc
    64801 ggaaagccgg gttcagagtt ggtgttattc gctatgtacc gaccactgat cttaatccat
    64861 atacagtgat tgatcagact cctaaaccgg gcggtttata tgataatcct attaacattg
    64921 atttgcaggt aaccattgaa ccataacaag ccgacaccgg aagtcgttcc ctctctcctg
    64981 tcggctgatt ttgcccgact gaaagaacat attcgcctgg ttgagaaagc tggagcgcgg
    65041 cgtctgcacc tggacataat ggatgggcac tttgttccca atattaccat tggacctctt
    65101 gtagtggaag ccattaggcg tgtgacgaat ctgcatctcg agacccattt gatgatcgag
    65161 agaccagagc gctttattca gcaattcgtt gaagccggta gtaatacggt aattttacac
    65221 gttgaatcag cccaggactt gcataacgat ctccgtaaga tcaaagaatt tggggtaaaa
    65281 gccggtgtag tactgaatcc accgacaagc tttgaggaag tcgaaccgta tttggatgaa
    65341 attgatcacc tgctggttat gacggttcat ccgggattcg gtggtcagca gatgattccc
    65401 gaggcgctca acaaagtacg gcgagctcag ccttggcagg cgcgctatgg ttttttgatc
    65461 gaagtggacg gtggcattaa ccaggaaaca ataacgatag ccgcggcggc tggtgccgat
    65521 ttattagttg caggcaacgc tgtctttggt agtggacgcc cgggtcatgc ctttcgatcc
    65581 ttaacctggc agataaagtg attaaaccgg tcaatgaggg ggaactcggt cgcaaggcga
    65641 ttcattattt ttcttccctt ataccaataa tctaccactt tttcttcaca agaaatgagg
    65701 cagtagtaat tatcgggatt ctatttatag gaatagttgt tgcggaattg atgcgtatgc
    65761 accttccggc actgcgtcag ctctattatc gtttgtttgg gtggatgatc agaccacatg
    65821 aatttcataa tcattttact ggagcgacat atgtatttat gggcgctttc ctggcaatat
    65881 ttctattccc gaaagaggtt gccgtaacga gtcttttaat tctgacagtt ggcgatccga
    65941 ccgcttgtct ggttgggctc tcgattggcc ggataaaaat ttataataac aagacccttg
    66001 aaggggcatt agcatttatt ttcgtatcac ttatagcgac ggtatggatt agctcgatcc
    66061 ccttctggat aaaattggtt ggcgctgttg tcgcttgttg tgtggaagtt tttcatcgca
    66121 agattgatga caatgtactg attcccctca tcagtgggac gacgattttt ttcctggttg
    66181 aattgggtaa ttttctaaat atttaattgg aataaaaagc gtattgagtt gtgaaatttt
    66241 atagcagaag acggtcgaga agaacgcctg actaagcgat ctcaatcaaa ttcttgccag
    66301 gtaattttgg gaataaagta gctgacttta gtattggcgg gatatgaaat cgtgatttcg
    66361 aaatcgaaag tctgtcccgg ctcaatcgta gcgtcggttt ggactccgct caaaaacata
    66421 tgataattac cggcgataat aatcgaatcg gcggcgatta gattagtagc ctcatcgaat
    66481 aattgtattt ttatctgcgg gaaatcggcg cgacgctcgc cctcgttttt aatttttccg
    66541 gtataggtgg tcttatcttc gaaaattttt ggcgttatcg gcccgactaa tacacacttg
    66601 gcatgtggtt tttcagcttc ccgaaagacg tgaatatcat tcttcgaaat ggtcagctga
    66661 ccgatgttag tctgaatgac gattttatca atagtttcat gaatgatttt gccctgcacg
    66721 acggtaccgt tatgcaagat gatttcatga gtgatgtcag gaacgggtat tagccggttg
    66781 atttccttcc ggatttccgg ggtggaagtg cgtagttcga tgtcgcggat ttgccggcga
    66841 atttcggcga tttcctggcg tagttcctcg atacttttgt ccacttcgaa aaattgatta
    66901 gtggtattgg agctggctga ctgagcgaag gaagaaactg caaatacggc gcaaatcatt
    66961 gggattagat ataggatctt ttttttcatt gagcctcccg agcttttagt tgggctttca
    67021 aagcggcgag tctgagttga ttgagaactt ccggttgacc aattgcggtg gctatctgag
    67081 ccatttcgtc atagagtaca tacccttctg ggctttccat aatcggcagg agttgcttaa
    67141 aagcctcgac gtcttggaat ttgaccagag ccgatagtgc attgcggcga gtagccagcg
    67201 ggtaatcagt gtttttcgct atttcgacca aagccggaat aacggccggg tcagaatagt
    67261 cgcccatagc tcgggtgaga gcgtccaaca gcaccaaatg ttcctcccga ctttggttta
    67321 gagtttccaa caacgctaaa ataacccgcg actctttgac gtcttcaatg gatttaatgg
    67381 caaatccgcg caatgccaat tgctgttccg ggtcgccaag catttcaatg aaggtctctg
    67441 tgattagtgg atgacgtttc ttgccgagaa tttccatcgc cagtgagcgc gtcgaaatgt
    67501 tgacgctttc atctttggcg atattgatca ggatgggaat gacgcggtcg tcgggaatcg
    67561 aacccagcaa cttagtgagc gattcttgca taaaaatgta gttttccttt tcggattgat
    67621 acagcttaag caatgcttcc acctgtagag caacgttcag atcttccatt ttctccatta
    67681 cgccagtccg gaagttaatg tatttcttct gggcggagac aatcccatcc accatagccg
    67741 caacgttttc cggtgtggcg tgcttcgtca aagcggttgc cgtggcattc agtaaagcat
    67801 aattgaggcc gaccgactga tccataaaat cgtgcatagc tttaatggca tccggatgtt
    67861 gtgtttcagc cagggcattc acggcggcga tgcgagtctc aataggttgt gcttggtctt
    67921 gatatattgc gattaagtca tcaagcttct ggatttttcc ccgattgtaa tcaacccgca
    67981 atttctcgac tgctcggagg tcaacggttg tcgtcttggg agcacaggac attaagccgc
    68041 cgagaaccaa catgataatt atgatgtttt ttgctttcat ttgtttcttc cgttctttat
    68101 ttatcccaaa tgattttaga acatctttat ttcttagtca agtaaaaatt ctgatttttt
    68161 accggtggaa tcagaggtgc tgagaggcca ataacatagg cgcagtgatt tgccatcaaa
    68221 gaatgcgaag gaataatatt tcatccagtc acccaggttc accacaactc ctctctcggt
    68281 atgatactca gtcggcaaat gtaaatggcc ggtgattaca tattcagcgc ctgcctccaa
    68341 ttttttcttg ccataggcaa tgatttcttg gcggtcggat tcttcctgag ccggactgcg
    68401 cagagtcaga cggcggcttc tcccagataa atgggtagct aagcggatgg cgcaatccgg
    68461 gtgaagatag cgaaataagc gaatgatggt cttattcctg aaaaaaaacc gcatcaaccg
    68521 gtatccgcgg tcattatgaa gaataccgtc agcatgggtc agatggaagc ttttcccggc
    68581 aattatcgtg tcaatcggtt tatagtgaac cgtaatgcca agcacgtccg tgaaaaattg
    68641 ccccgcccaa taatcatgat tgccaccaag aaagtgcacc tcgcatccgg ctaaaaccgt
    68701 ttcgcgtaac tcgcataaca ccgggaagaa tacgcccgga atcacataac gatattcaaa
    68761 ccaaaaatcg aacatatcac caagaatata taatgaacct tggtgacgtc ggacttctgc
    68821 cagaaacgca aaaaggcgtt cgcgttttac cttttcttcc tgactatcgt ttaggtgcag
    68881 gtgaatgtcg gcgataaaat agacgttttg attcattgtg tttaaaattt aaaagaatag
    68941 taagatatac gaaagactat ttctctactc ctgctcgccg attgatttca ttttatggga
    69001 aaataaatta gataattcct aaaatttaga aataacagcg gtcccttctg tctcttttaa
    69061 aatgtacttc acgatgataa agtaatcaat ggcatcatat ttgtctggca atgggttaga
    69121 gatgtctgac aaatacaaat aagaagcaaa gcccagtatg atagaaacag tcttggtggt
    69181 aaatcctggt tcgacgtcaa ccaaaatcgc gctatttgat cggagtaggc gactccaatt
    69241 cgaaaatatc agtcatgtcg gtactcccat aagtcggatt cactcgatcg cggagcaatt
    69301 acccttacgc atcgagattg ttactaatac aattaagccc tggttatttg attattgcct
    69361 gaaagcagtg gtcggtcggg gcggtcttct caaacctctg cgggcaggtg tgtataaaat
    69421 taccccggga atgattaccg atgcaatcag ctgtaaatat ggggagcatg ccagtaattt
    69481 aggggctccg attgccgaag tgatcgctaa tcaacatcat atttccagct ttgtggtgga
    69541 tccagttacg gttgacgaaa tgattccgga ggcgcgcatt tcaggtattc ctgaaattga
    69601 aagagtgagc cgacaacaca ctttgaacat tcgggcttgt gtgcgcaaag ccagcgcgca
    69661 actgggtcgc ctgcctagtg aattgaattt tgtggtcgcc catctgggag gcggtgtcag
    69721 catagcggcg gttagaggtg gtcgtctgat tgattgcaac aatgctttgc ttgggatggg
    69781 tccttttaca ccggaaagag ccggagcttt gcccctggaa ggtattctga atttggcgta
    69841 ttcagggaaa tacactaaag aagaaatgaa aataaaaatg accaaaaaaa gtggtctggt
    69901 cgcttatctc ggtacaaacg atgcccgaga agtctggcaa cgagcagaga cgggagatca
    69961 gcgggcggca ttgatattca gggcgatgat ttaccaggtt tgcaaagaga ttggcgcgat
    70021 ggcgactgtt ttgaagggaa atgtggacgc aataatcgtt accggcggac aggcacgtgg
    70081 tgctgaaacc gtcgagatga tccacgctgg ggtgggattt attgctccgt tgattgtttt
    70141 cccgggtgaa aatgaaatgg aagcactggc tgaaggcggc tggcgagccc tcgatgggaa
    70201 tgaggaaatt ctgatttatg aagagattaa tgtcaaagaa taacttatgc taaagaccta
    70261 ttccgaaata tttgaaaaaa ttaaaaaaaa tgctcgtaga ccgacgatag cagttgcatt
    70321 agctgaagat aaaagtgtca tggaagctat taaagtccta tcggcaaacg atatcgcccg
    70381 ggttatttta accggcaatc cgaagaaaat taagtcgtat gctgaacagg ttggttatcc
    70441 cataaaagaa gatgaagtaa tcccggcatt tagcgaggaa gaaggagcca cacgggcggt
    70501 cgaactggtg catttaggaa aagccgagat cctgatgaag ggacatatta cgacgccagc
    70561 tttaatgaaa gccgtattgg ataaagaaaa aggcttgcgc agtggggatc ttttaagtca
    70621 tatcacggtc gttgccgttt ccaactatca taaattactc atggtcaccg acggcggagt
    70681 taacatagcg ccggatctcg agaagaaaaa agctataatt aacaatgcca ttattttcgc
    70741 caaaaagcta gaaatcacca gaccaaaaat cgcagtttta gccccaatcg agaaggtaaa
    70801 tcccaaaatt ccggaaacag tagatgcaat gactttacaa gaaatggctg agcggggtga
    70861 atttggttcg gcaattgtag aaggcccagt agcggctgat gtcgcgctct cagcgagagc
    70921 cgccgaacgt aaaggtttac atagtcgcat tgccggtgat acagatatta tactggtacc
    70981 gggtattacc tgcggcaatg ctattatcaa gtttttaatg ttgctggcta aggctgaagt
    71041 cggcggagtg gtagtaggtg ggcgggtgcc gatagtttta ctttcgcgtt ccgacgagcc
    71101 agcggaaaaa ttgaactcga cacttttagc tcttctaatg agcgccctat aatttatcaa
    71161 tcaattaacg aaaattcccg cggcgcagaa acttaaatcc ctcagatttt gtaaattttc
    71221 ctgcgtttga aatttaattt taacaaaagg tgaaataatg agttttgaaa aattaatcaa
    71281 accgggcact gaatttttgg gcgtcaaata tcctatcatt tgcggcgcta tgacttgggt
    71341 gagcgattat aatctggtaa aaacggtcag tgaaaccggg gctttcggag tactggcagg
    71401 cggcaacttg cctcctgatt tattcgagca agaagttgac cgctgtattg ctggtgtaaa
    71461 gaagccgttt gcggtcaacc taatcacgat cgggcctaat tttcgaatac attatgaaat
    71521 tttacaaagc aagaacgtcc catttgtagt ttttgccggt ggtattcctc gccgtcagga
    71581 tattgagggg atgaaaaccc gcggcaaacg aacgctatcg ttcgctcccg aagaaagcat
    71641 agcgcgccaa cagattgaat atggcataga tgctctgatt ttggaaggca gtgaagccgg
    71701 tggacatatc ggttatgttt cattgattgt tttacttcag caagtcttgt ttaaagtaaa
    71761 aggtttgccg attttcgtcg gtggtgggat cgctgatggg aggatgatcg ctcacttgct
    71821 tctaatgggt gcggctgggg tccaaatggg aacccgcttt gtgttaactg aagaatgtcg
    71881 ggttcatccg aattttaagg cggcattact gaaagccaaa gcgcgccagg ctattgctac
    71941 tccacaatat gattccaaat tgccggtcgt tgccgtacga gctatcaaga acaaaggaac
    72001 ggatgagttc gggaagctcc agatgcgttt actacaggaa atcgctgccg gtaagattac
    72061 gcgggaaaaa gctcagtatg aagtggaaaa tttttgggtt ggcgctttgc gtcgggcggt
    72121 ggtggagggt gatgtagatt atggctcgtt aatggccggt cagagtgttg ggctaattaa
    72181 cgagattgtc ccgatgcaaa agatgattaa tgatctgata aaagaagcgc gcgcagaaat
    72241 aaaagttatc cgcaaccgtc tgggacagtg cggataactt ttggattttc cttccggatt
    72301 attgttgttc cagcttctgc agctgattac gggcaagtaa tgcttcttca gtattgggat
    72361 attcccgaat aactgtttcg aactgggtgc gtgcctgctc caagttgttt agttttaaat
    72421 agcataatcc cagtttgtaa tgggcgttag tgattttctg actgcgggga tagttattaa
    72481 tcaatagctt gtattcggtc gcagctgtgg cgtagtcttc ctgttgatag gcgatttcgg
    72541 caagataata ttggcaataa tcggctaatt ccttatccgg ataaagtttg aggaaagtct
    72601 ggaagccctg aatagccagt tcgatgtttc ctttagtcat atctcgataa gcgttgttga
    72661 aaatttcggt aggagccggc tcagccgagt tgggagtggc ggcgcggctc tctatttttt
    72721 ccgaaatatt ggtcattttg cccgtcaaag attcgacctt gccgatcagc tgactggagc
    72781 ggtagcttgc gtcgtccaat ttactgctga taatcgccgc atcttgctta aggctggata
    72841 gtcgggtatt gatatcagct cgggttcggt cattggcatc ttgtaattcc gcaagggatt
    72901 tttccagaga atctagctct atcaatattt gattgcattg aaccgcaata gtaggaacct
    72961 gacttaacaa ttgtttattt tcttcagtag tctgtttaac ggattgaatc tggttgcgta
    73021 aaaaatataa atcctcttga attttcaaaa gatctttttg agtggcgcaa gccgtcaagc
    73081 tgatcacgaa caaaccataa atgattggac gaattggttt aaagttcata gctaccttgg
    73141 ttgatgaaag cgccgagaat ttattttgag tccgaagtga ccaataccgc tcggcggttt
    73201 ttagcccaag cctcttcatt atggcccgga tcgatcggtc tttctttgcc atagcttatc
    73261 gtaattagat tgtccggttt tattccataa ttgatgagat aagtttttac ggcgtgagct
    73321 cgacgctcgc cgagtgccag gttatactct acggtgcctc tttcgtcgca gtgaccttcg
    73381 atagtgataa tggcgttcgg atactgctta agcactttgg cggctttttc gagagtggcg
    73441 atggcatccg gtcgcaagtc ataacggtcg taatcgaaat agaccgtatt gcattgcgcg
    73501 agttcatctt ttagggaagg ttcagcaatg gcggccacct tttgtgtgtc cgctatgaca
    73561 ggtttgggtt cggcaggcgg tggggtgact acaggggctg gtgcttgggc gagagtatcc
    73621 ggcttttgga caggtttagg ctgttccggg gccggggtga ctttggctgg agtctcaccc
    73681 ttgtcttctt gcacccccgc ggtcttttta ctgcaagtat tgaacattaa tacaatacag
    73741 agtaaaggaa ttaaaaaaac gagaagtttt ttcattttgg ttgctccttt ctttttgtta
    73801 attagtgtta tactcataca ccctgaaatg attttctagt taaatggttt caagcgcggc
    73861 gaccaggctg gaccggtatt gccaccggtg aaagttaatt ggcgtaatag tgatccgtcg
    73921 cgcaacatgc tatagatttc ccagcgtcca ctccggttgg aggcgcaaac cagatggcta
    73981 ccattaggcg accaggtagg attttcgtaa ctaccggtat tatcagtcag gcagattaga
    74041 ttttctccgg taacatcaat gagatagatt tgaaatccgc gggattgata agaaacaaag
    74101 acgatttgat cgcctttcgg tgaccattta ggtgaggaat tatagttgtt ctcataagtc
    74161 agccgacgga cattgccacc ttcggcatcc ataatataaa tttggggaga accgctacgg
    74221 tccgaagtga atacaatttc gcgactgtta ggtgaccacg acggggctgt atcaatagcc
    74281 ggattgctgg tcaggcgctg gagattttga ccgttacggt cagcagtgta aatgtcggca
    74341 ttgccctcgt gagtaaggac aaaggctact tttttaccgt tgggagaata ctccggcgaa
    74401 gtaaatgtgc cggcgcgcga gatgaatcgt tcaatttgct ggtctttgaa tatataacgc
    74461 gccagaatgg gaccgccacc aaaagcgtat gtagtaaaaa ttaatgattc tccatcgttc
    74521 gaccagcatg gcagtaaatt aattgaacga tttcgggtca ggagagtcgc gcgctggcca
    74581 tcccaatcca taagggctaa ctccttgccc cctttttgtt cggtaacata agcaatttta
    74641 gtgcaggcat tgccatattc accgcataat tggtacacga tttcgtccga aacggtatgg
    74701 gcgagaactc gcagagcaaa ggggtaagag cgcaaagact tggtcattag tttccgaccg
    74761 gtgacagatt cggtaagccc gaatacaaaa ttgaaataac tactcttgtg agtaacatca
    74821 ccggtgataa ttacctctga attatcaaag attaaatctc cggcagggct gatcagcgga
    74881 tcaacaacat tgagaaatcc cgaataattc aaatcatcaa taataatctg gcgtaaatta
    74941 atagccaact ctggattttg attagtaccg aattcagcga tagcgacatt aattttgcgc
    75001 gtctcgtccg attgaatttt aaggaacaca tctgtttgac tacttccagt gacgttcaag
    75061 aaggatatta ggcaaatcaa tgatagattt tttttcatac cacgtttatt tcaaagtttc
    75121 aaattcgata tgaacattta actcttttcc gccatattca ctcggtaagg gtggtaaagt
    75181 agccgtttcg taaagcgcac gcagagccgc ctgatcaaag agaaaattac cggaagattg
    75241 ttcgattcgt acatcggaaa tttttccgga acgggtgatt ttaaaggcaa tcataacttg
    75301 ctgactgtag ggagaatctg ctgaagaata gggaggccgc caatgttcct gaatccggtt
    75361 gcggataatg ttaagataat agataaaatt gaagtcggga tcatctaccc ttaaaccggt
    75421 tgcaccggga ataccgccag acgcgggctg agatgtagcc tccggctcga cgggtagctc
    75481 cggtgaggtc gtggacgagg cgatcgtttc ttcaggtaac ggtttggtct cttggggctt
    75541 ttgtggtttt tccgcaagaa cagtagcagc tttttctact ttgggtttag gaactggaac
    75601 ggaagtggct ggttttacct gaattggagc ttcagcagtt tgaacgaccg gtggagcgat
    75661 attgacccag cgtatgttct gatccgggat ggccataacg cgaggctggt ggcgaccagc
    75721 caagacactt acgacggcaa aaaacgtcaa gtgtaaaatt aaggatatgg caaaagtgta
    75781 attcacttct tatcctgttc ggtttcgact attaatccca attcggtcac gccgcaagct
    75841 tttatccggt caatcagctt gataatttta ccatattcaa ttttccgatc ggcacttaat
    75901 aaaaccggct tcttaccgac ttgtacccag cgtttgacta aagttttttc aaattcattt
    75961 tccattaccg gttgcccatc aataaatatt tgcccatcaa gactataatt aaccaggatg
    76021 ccttcttgga cagtagtaat ttcgcgtctg gtagcaggca aatcaacctc tataccacta
    76081 tacattaagg gtgtcgtaat cataaaaatg attaaaactg ttaaagtgac atcaactaaa
    76141 ttagtaatgt tgatttcggt cagcggctta tatctgcgat ccaccataaa aattttcccg
    76201 gatgatcttt tgaatcatat cattggcgag aatttgtaat tgattctcgt attttcggat
    76261 tttatctacg attaagttat aggcaaaaag taccggaata gcgactgcca aacccacaac
    76321 agtcgtaatt aatgcctcag caatcccggg acctatggct gtaatcgccg ctgagccttt
    76381 tactccaatg ctcaagaaag cttgcatgac tccccatacg gtaccgaata atcccagaaa
    76441 cggactggca ctaaccgtag aagacaaaaa tatcaatctt ttttcataag gtataagttc
    76501 ggtgctaatt gcggaatcca ccagggcttt ataaagctcg agcgacgata tgcgactggc
    76561 atcggtatct atgttctcag taacaacgtt gcctggattg agctcggcat ctttgcgggc
    76621 ttcagcgtat atttttcgaa aaagattgag aagtaaaccg gacttgagat gctgagtttc
    76681 cctcgcgacc gcgctccagt cggaatacga ctgataaata gccagaaatt ttttattttg
    76741 tttttcggtg cgtctgaaga gaattatttt ctcaataata ataccccagg cgatgataga
    76801 aagcccaatc aagatcagaa taattaccca tcccattatg ccgatatcat aagtagaaga
    76861 gaaaatggta ttcatgtttt taatcctgtt tttactgcta aaatgactgg tttaattgaa
    76921 tattccatat taataataat gaatctgcat tataagataa attacgcact aataagttaa
    76981 aatactatga tcagcgtatc ccacataact tgtgatttag cgtaaatatt tctcgagcca
    77041 gctaaaaatt gtctgatgcc agaaacggct gttttgtggc cgcaacacga aatgtccttc
    77101 atcgggaaaa agtaccagct tggcaggtat attcaagcgc cgcaaggcgg tgaaagccat
    77161 tattccctga cttggatcta agcgataatc cttttcgccg tgaataatca acataggagt
    77221 tttgaaattt attatgtaat tatcgcagga gaatttttca tattgatctc gattttccca
    77281 gaagacacca ccatattccc attcggggaa ccagagttcc tcagttgtaa gatacattgc
    77341 atataaattg aatgaaccgt cgtgattaac gagggttttg aaaggatacg aaaacgcctc
    77401 catatgaccc tccatccagt tcatcatata accgccgtag gaagctcccg ccgcggctaa
    77461 tttatcggca tctatgaact tgaagtggtc tattacatat ttttgtccaa cgacgaggtc
    77521 atcaaaaacc tggccacccc aatccagact aattgcgtcg gtaaattttt gaccatagcc
    77581 aatactaccg cgcggattaa tcattataat tacataacct tgagccgacc atagttgcgg
    77641 attccaacgg taatgccaat tatcctccca ggcgctctgt ggcccaccgt ggaccagaaa
    77701 aacagccgga tatttctccc cggcatcaaa gaaaggcggt gtgattaaaa agccatgaac
    77761 catcgctccg tcactgcttt taaactcaaa atcctcaacg tcattcattt cgatttgaga
    77821 ccttagcgga gagttgaagt cggtaatttg ccggattttg ttatcggtga aagaatatac
    77881 gaaaagttca tcggggctgg tagtccgcga ctgtaaaaaa gcacaagacc gcccgttagg
    77941 agccagcgtt actgcgctgt tatagccttc cttgaccagg gtcttaatct ggcctgattt
    78001 aatattcagg gtgttcaagg ttagccgacc gcgttcttct attaataaga gcaaaccacg
    78061 actatccgga aaccattcgt aggcttgaat ccggatatcg tttttttcgg tcagattgat
    78121 aaatttgcca gtagccattt catacaagaa tgcatcggat ttatcggctt cgaaaccggc
    78181 acgtaccatc gaaaggaaac ttagatattt gccgtccggt gagaatctgg gagcgtaatc
    78241 attgccgcgg aagtcacggt actcctgggc ggtggtaatt aatcttggtt cgcgcggatt
    78301 agcaattgag actgaccaga tgtcgttatt ggtgctaatg gcgatgaccg gatcgtgatt
    78361 actattaaaa acgattgttt ggtcatcggg agaaaaacag tagccttcac ccagcgctat
    78421 gggtggggca tcaaaatcgc caggcgtaac gtctgttaaa ctaccgttat tcaaatcaag
    78481 cacgaaaatg tgactacgtt tataatccca ccactcgtcc cagtgacgaa acattaattc
    78541 ttcatagacg cgggctttga ttggactagc ttgccgggcg gtgtcgcgtt ttttcatctc
    78601 ggcgtaatca gtcaaatccg gatagacatg ggcagagaaa gcaatctttt gaccattgtg
    78661 cgaccagcaa aaactttcta ccccgccata aaagtcagta atttgttgaa cgtgattatt
    78721 atccaacgcc agtttccaga cctgaacctg tccgccgcta tcggataaaa atgctaaact
    78781 tttactatcc ggtgaccaga gtggcgacca gctattgcca gaatgggtag gaatctgttg
    78841 taaattttgg ccatcggggc ggatacgcca cagtgttttt tgccctttat tggcttcgat
    78901 atctatggtc tgaacggtga aggcgatcca ttgtccgtcg ggtgaatact ggggttctgc
    78961 cagtcggtga caattcagta agtcatcaat ctgcatagag cgcttagcag tgaatcctac
    79021 ttccaccatt atgattagag atagccataa taatccgaca gcttttgatt tcattggtaa
    79081 ctcccttata tataggtttt agatgctttt ttggggcata aatagcgata aattgacggg
    79141 ctgaaaacgg tgattataga tatatttacc gagaaaataa aaaagtgtgc cggtgataat
    79201 tccggcggcc gcatctatgg cataatggta actgcaatat acggtggaga ttataattcc
    79261 gagaca
//
LOCUS       DAOSMP010000002        76714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_345_length_76714_cov_17.258352, whole genome shotgun sequence.
ACCESSION   DAOSMP010000002 DAOSMP010000000
VERSION     DAOSMP010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 76714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 76714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76714
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_345_length_76714_cov_17.258352"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1397)
                     /locus_tag="PKX09_00355"
     CDS             complement(144..1397)
                     /locus_tag="PKX09_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOU16320.1"
                     /translation="MEVTLTVILIGVIIFCAHLFSAIYSRTKIPDVLFLFLIGLLIGP
                     ILGLVQPAHFGAVGRVFTTITLIMILFESGIGISLRGIRQSLKGLLSLTILNFIVSVI
                     VVGGLVFLFTRLGLIQSLMVGAILGGTSSAIVIPIIDQLDMLEDSKRILIFESIINDV
                     LCIVFTFSLLQAYKLGEIKIGLMTLKIIATFIIAAAFGVLGALAWSAILNRIRNLENS
                     TFTTLAFIFIVYGIVELLGFSGAIAALVFGFTISNVSKIKFSMFRYSGRLETTELDSR
                     ERGFFSEISFLLKTFFFIYIGISIQLSGIGLLVFGLLLTIAIYAIRIPVVSLTIPRGT
                     PIKDATLFSVIIPKGLAAAVLASVPLQQGVVGGEVIQNLTYAVVLLSIIMTSVLIFLV
                     DKTKLAEVYQALLGKFNKSMSANAH"
     gene            complement(1498..2490)
                     /locus_tag="PKX09_00360"
     CDS             complement(1498..2490)
                     /locus_tag="PKX09_00360"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOU16321.1"
                     /translation="MKNEFPTLDFENNLWKQGVSHIAGVDEAGRGPLAGPVVAAAVVF
                     DKSSEIISGIQDSKQISPRKRERLFDEIMSQAKAIGIGLVESEEIDRINILRATHKAM
                     RMAIGRLKINVQHILVDGKPLPDKIYPQTAIIGGDRKCYSIAAASIIAKVYRDRLMQQ
                     YDSVFPGYGFAEHKGYGTKKHLESIVKLKPCPIHRISFRGVTEYIVDINKSANTRWLG
                     KYGEDLATLYLFRHGYQIIQRNFHLGAYGEIDIIAQKDNNLCFVEVKTQRHTKFGPPE
                     GWVDERKMRQIGRIASGYLSQNPVENVDCRFDVVTVNLNGSEVQIEHLPDAFRL"
     gene            2907..4883
                     /locus_tag="PKX09_00365"
     CDS             2907..4883
                     /locus_tag="PKX09_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOU16322.1"
                     /translation="MKEFTIRSLIIGLIMAVVLGAANAYLGLKAGMTIAATYPAAVIG
                     MAILRMMKGTILEENFARTVGSIGESVAAGAIFTIPAFYIAGIWTDFATLPNYLIASS
                     IMLVGGVLGVLFVALLRRVMVEDVELPFPESVAAAEIHKAGQKGGTGAKFLFGAMGLG
                     AGIQALISLKVFTDLKDWFITFKKAIIQLGSSGSATGQSGMVLTAPRVSPAYIGVGYI
                     IGPKLASLNFSGGLLAWGLFVPILTYFIAPSILPDTTAVTTDTWMALSSQVWRNLVRP
                     IAVGGMLMSAIYTLFKMRKSLINGLSRSISDVKKAAQGVQVNERTEQDLKFTTVMFGI
                     SLAAVGTFFIYLFFAKNVIAALVATVVMIIAGFFFAAVSGYLVGIIGSSNNPISGLTL
                     STLIVAALLMVALGMKGTPGVAAVLGVAGVVCVSAAVAGEMLQDLKVGHILGGTPWKM
                     QIGDLIGVALSAVVMFIPLIILHQGDINMGGTGLGGTNLAAPQASLMAMLSQGIVSGE
                     MAWPLIIVGILLGLGFILMQVKSPMLVCVGMYLGLETTFAIFVGGLIRGLLDHYVTKR
                     RCNDAQKSRVENTGVLLAAGLIAGEALVGLLVALLAILKVGYDKWLPSIFNFLPFRFE
                     VSLLIFGLIGWILVSIPLKNAGKADEPAPPSAVM"
     gene            4902..5243
                     /locus_tag="PKX09_00370"
     CDS             4902..5243
                     /locus_tag="PKX09_00370"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOU16323.1"
                     /translation="MKNKKGEVLNLYEIVPRPLVNSECQADGRIVLLKPKFKNPFLIK
                     LLQRNQRDNFYKLKLDEIGSACWIKFDGYKSAGIICEELESQFGAQIQPVAERVGKFL
                     IMLKKANLIAY"
     gene            complement(5562..5765)
                     /gene="rpsU"
                     /locus_tag="PKX09_00375"
     CDS             complement(5562..5765)
                     /gene="rpsU"
                     /locus_tag="PKX09_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOU16324.1"
                     /translation="MVEVTVYDNEPLERALRRFKKKFEKAGILNDVKKRTYYEKPSVE
                     KRMRRAKAIKRLHRTNAQKTAIM"
     gene            complement(5789..6646)
                     /locus_tag="PKX09_00380"
     CDS             complement(5789..6646)
                     /locus_tag="PKX09_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOU16325.1"
                     /translation="MSKKDKRVGSDSNRALSKYLEEIGKFEPLSPDKEIELAQRLRAG
                     DRDALRELTESNLRFVVSVAKDYQGQGLPLTDLINEGNLGLIKAAERFDETRGFKFIS
                     YAVWWIRQSILQALAEHSRIVRLPLNRVGTISKITKEAEILEQQYERAPMQDEIASGL
                     AMESEEVSDAIRISRKHQSLNDPFRDGESNALIDVIEDDMQLPPDHPLMDESLKQEIR
                     AALNTLKKREREVIKMYFGIDREYALTLNEIGEEFNLTRERVRQIKENAIRRLSHKSR
                     SKNLKAYLG"
     gene            complement(6807..8813)
                     /gene="tkt"
                     /locus_tag="PKX09_00385"
     CDS             complement(6807..8813)
                     /gene="tkt"
                     /locus_tag="PKX09_00385"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOU16326.1"
                     /translation="MARLCANTLRLLAVDGVQKANSGHPGMPMGMADVATILWSKYLE
                     HNPGDSQWANRDRFILSAGHGSMLLYSLLHLSGYDVTLEDLQQFRQWDSRTPGHPEYK
                     CLPGVETTTGPLGQGFATGVGMAIAAKITASRFNTDQFQIFGTHKIYGIVSDGDLMEG
                     LSHEAASFAGHLKLGNIIYFYDDNRITIEGKTDLTFSESVAKRFEAYGWEVLQADGHD
                     YISIAKALDEALSKPDRPKLIITRTHIGCGSPSKQDTSECHGAPLGEEEVKITKRNLG
                     FDENVSFYVPEEVRTVYAERRAELEKKYAQWQIEYQKWREANPELAKKYDESQNQILS
                     IQQEEEIIKAIPPDALATRALSGKIMQKIAELLPNFYGGSADLAPSTSTYLKNLGDIA
                     PDNFSGRNFHFGIREHAMGAILNGLALYGGLIPFGATFLVFSDYMRPPIRLAAMMKLR
                     VIYVFTHDSIFVGEDGPTHQPIEQVASLRIIPNMTVIRPADGLEVAQAWISAINNLGG
                     PTALILTRQKVPVLNRLANFDRHLIDRGGYIISPEQNNALDLVLVASGSEVSVAIEAQ
                     KILQAEGRATRVVSMPSIEKFVRQNATYRESVIPKGKVPVVVIEAGRSTGWGDLIRNP
                     LLTITIDRFGASAPAKVLAEKFGFTAQQVVDRIRDWQVLTNIIV"
     gene            complement(8833..9927)
                     /locus_tag="PKX09_00390"
     CDS             complement(8833..9927)
                     /locus_tag="PKX09_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010867978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOU16327.1"
                     /translation="MVNVTELDNFVRQVLIAHGLNAEDARITAEVLIRADRRGIGSHG
                     VARLKRYVDGLDSGVIRPNPDVKIIRETPVSLVIDGDGGMGQPIAYRAMQKCIEKART
                     AYMCFAAIRNSNHYGIAGYYVLMALAEGMMGVSLTNTTPLVVPTFGRNVTIGTNPIAI
                     GFPGKEERPFLLDMATSTVPRGKLEVYARQGEPIPDCWACDEDGKPSTDAAALLDDMI
                     NRRVGGLLPLGGGTELTGGHKGYGLSAVVDILCGVLSAGMVGTEIARYSNAPAGVSHF
                     IGAINPDAFVGTDLISEGIDKYIQMLRNADKAAGQERIYVAGEKEFLAEERYRDFVPL
                     QDKVFQTLNDIGTKVGLSITELKKERTKCE"
     gene            complement(9961..10824)
                     /locus_tag="PKX09_00395"
     CDS             complement(9961..10824)
                     /locus_tag="PKX09_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOU16328.1"
                     /translation="MVHKQKIKNIIFEYCGLAVGSAVMAGSLVFFLIPAKIAPGGVSG
                     LSIVLHYLFNLPVGLVMLVFNIPLFFLGLKILGKEFGPRTFFSFVLVSIFTDFYDKVL
                     GVQAATEDPLLASIFGGILLGVGLGVVFRFKGTTGGSDIVGQIIHKYSNVSVGTGILI
                     ADFFVITFAGITFRDVNLALYGFISLYFSSKVLDLILDGMDYARSFYIISEKQDEIIN
                     LITKEMDRGGTELLGKGFYTRKTRNVLYTVVTRREVATLRQEILKIDPKAFVIIANVH
                     EVIGEGFRKRI"
     gene            10994..12181
                     /locus_tag="PKX09_00400"
     CDS             10994..12181
                     /locus_tag="PKX09_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU16329.1"
                     /translation="MQNNQNPIDNKENKRTVRIFAWASFLNDMGSDIIYPVWPLFVTE
                     ILKANMAVLGFVDGLGEAVVSLAKALSGYWSDRIRKRKVFIWSGYLMGAASRIGYAIS
                     TVWQHLIPFRILDRAGKIRGAPRDAIIADVSSNDNRGRNFGLMRMADNLGAVVGILIC
                     LALFKKIGYRSLFILAAIPSLISVLLILIKIKEPGHTEKVIYKGMTLRDLDRNFVLFM
                     ILSGIFAIGNFTYSFLLIRAKQIGFQEAFVPVLYLVYTLSASLTAYPFGRLADRIGRK
                     TVMQMSFLLWGLVCLDVIFFQSKIMIFLMFVIYGLHKGALEPVQSTIVSELAPEKFRA
                     SSLGTYQMLIGLCAFPASLIAGFLWDKIGALAPFYLSLGLTIVASALMIFVKESKIIT
                     KSN"
     gene            complement(12182..12646)
                     /locus_tag="PKX09_00405"
     CDS             complement(12182..12646)
                     /locus_tag="PKX09_00405"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOU16330.1"
                     /translation="MEVNDTNEVKPKPKNIALVAHDHKKKDLLEWVKFNEGTLKQHKL
                     HATGTTGKLLEKELNVKIHKLQSGPLGGDLELGAAIAQRKIDVLIFFWDPLEAQPHDP
                     DIKALLRISVVWNIPVACNRATADYIISSPLMNSVYQPTLPNYSEYLNRLKK"
     gene            complement(12685..13557)
                     /locus_tag="PKX09_00410"
     CDS             complement(12685..13557)
                     /locus_tag="PKX09_00410"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOU16331.1"
                     /translation="MKIGLLLSPKIELNDPIVPAIIKLLIENKQTVIVADWQSNSLVT
                     NLNGVERCNPEQLPEKIDALFSIGGDGTFLSASHLMAKSRKPVLGIHLGGLGFLADVS
                     VDNYHERLIDFLNGKYSVEERQGLTARTYFKDSIQESFAFNDFVIDKGRISTMIKIRT
                     YINEEYLNTYRADGLIIATPTGSTAYSLSAGGPIISPELNVLVICPICPHSLSVRPVV
                     ISADQVVRIDCTDLSDDVSLISDGHNRIALGAAQKIEIHKAEFPLRIVRFSGDSFFHT
                     LRTKLNWGRDVRGN"
     gene            complement(13570..13833)
                     /gene="xseB"
                     /locus_tag="PKX09_00415"
     CDS             complement(13570..13833)
                     /gene="xseB"
                     /locus_tag="PKX09_00415"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOU16332.1"
                     /translation="MAKKSFSLEKSLERLEEILQILESGEKDIEESIKLFEEGMRLSS
                     ECQAHLQALEKRVKLLIEKSDGEICEQDFPESGETPETNNLFD"
     gene            complement(13854..15062)
                     /gene="xseA"
                     /locus_tag="PKX09_00420"
     CDS             complement(13854..15062)
                     /gene="xseA"
                     /locus_tag="PKX09_00420"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOU16333.1"
                     /translation="MAERIYTVTEIARDIKRVVEQFIAPVWVEGEVSNFTISRAGHIY
                     FSLKDQTAVLNCTIWRSQSLSIPFQIVNGMRLLVFGNITTYAMQSQYQLNVQQVRAAG
                     LGSLFLAFEALKKKLSAEGLFDGERKKPIPLYPTRIGLITSESGAALRDFLQISRRRN
                     PSIAIYIYPALVQGAEAAATIIAGIKLFNRLRNVDFIVITRGGGSLEDLWAFNEEPLV
                     RAVAQSAIPIVSAVGHEVDFTLCDFAADLRAPTPSAAAEMTIPERSHILNFIDQTCNK
                     MAQNIRMRLSNFQKEIDNIARRLFLNRPLELLRQRIQRIDELEERIIKAINSRILSAQ
                     VELDNIQKTLVVLNPRAIMERGFAIVYHWPDKKIIKSISEVAVGEQVSVELKDGEFQA
                     KTEIIQSHKN"
     gene            complement(15250..15567)
                     /locus_tag="PKX09_00425"
     CDS             complement(15250..15567)
                     /locus_tag="PKX09_00425"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOU16334.1"
                     /translation="MYRKFGGENMTKADVVDIVARATGLTKVDTEVVINGFIETIKDA
                     LLNGERVDFRGFGNFSLKVRKPKKARNPGTNEEISLPARIVPVFKPSKLFKGEISRNY
                     NQH"
     gene            complement(15819..16214)
                     /gene="nadE"
                     /locus_tag="PKX09_00430"
     CDS             complement(15819..16214)
                     /gene="nadE"
                     /locus_tag="PKX09_00430"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOU16335.1"
                     /translation="MYAELENRLVVGSTNKSEYQTGFFVKYGCDSANDILPLLNLYKT
                     QVRELARYLNIPAKIREKAPSPDILPGLTDEKALSISYEKLDLILIAFAKSWEVTEIA
                     GALEISEKQVIEIERLIQKSEHMRKIYAP"
     gene            complement(16184..16660)
                     /locus_tag="PKX09_00435"
     CDS             complement(16184..16660)
                     /locus_tag="PKX09_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16336.1"
                     /translation="MKKLEREGVIIGLSGGIDSAVIAALCKRAVGAENTLALILPEKD
                     SRREHQVDAQIFANELGIATKLIDLTPYLEMLEVYKLSPVNKPFLPEKIKGTLIGKAY
                     DYYRRKTGETPFSASLTGLRDKEFDSYLKKAMPIIGLNTDCECFYFICMRSLKIGW"
     gene            complement(17092..17955)
                     /gene="lepB"
                     /locus_tag="PKX09_00440"
     CDS             complement(17092..17955)
                     /gene="lepB"
                     /locus_tag="PKX09_00440"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOU16337.1"
                     /translation="MKEQKQEKKTSGSKFWQEVKSWGIVLLIALGLKATIIEAYQIPT
                     GSMENTILTGDFILGIKFIYGTRTPDWIGIPWTKTGFHIPWFRLPSFKKPEQGEVVIF
                     KYPVDPSLNYVKRCIAGPGQSVEIHDKVVYVDGKIFPNPKHAKFISQYVAPSGWNDPE
                     IFPDGIGNKDNYGPIYVPAKGDTLRFGQIQTGIIKNAAELKGHTFRIYQGKMYIDGNP
                     AEYYIAEQDHYFMMGDNRDNSWDSRFWGFVPFDNVTGRGLITYMSWDKERPLYQLDKK
                     IRWWRIGRLVR"
     gene            18395..19888
                     /locus_tag="PKX09_00445"
     CDS             18395..19888
                     /locus_tag="PKX09_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOU16338.1"
                     /translation="MVKNVVDILIQNRNNRADWPAVIFNEQTFTYSQIYDRSIYLAEF
                     LQKQKIQAGQTIGLLFQNKPEYLIAYYACLLNGFLPVLLPTYLSTNKLTYNFNKLKIE
                     AVLYDSEFHKVVDEIELKKGSPLIKVPSSTWQDTSIDKTNIPTLSTDSFPGINYTNEP
                     AVVSFTDGNSGFPKAVVHSQAGLLANAQNCVKLFGGMRTVRLLCTVPVFHFISHSFIP
                     HSISLAGGTVILVENSKTEEIIRAINSNKVNTLIGTPAFFKEFINYAKTDELSSLKHC
                     IVSGSCLSQEIRRNMIKNLGVFVSELYGTTETQMISVVYDEIDQCEGSIGQPFPEIDI
                     RIINSSGAIVTPGSVGELQVRSSALMLNYFDDLDKTHFNQTTWFSTGDIVRKTSDGSL
                     IFEGRIPDSIHKFGYDINPALIEKVLRQHPAIHEAVACPIKNPNHEDQIKVWVSLRES
                     ATVSIDDLHDFCRKYLPGYLQPDAIEVVTYFERDLSGKILRNLHNKS"
     gene            19916..21994
                     /locus_tag="PKX09_00450"
     CDS             19916..21994
                     /locus_tag="PKX09_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOU16339.1"
                     /translation="MKEYQTEEIRNIVLLGHGSSGKTSLSEAMLYSAGATTRLGRVDE
                     GTTVSDYHDDEIERKFSISSSLLNCEWNKIKFNILDSPGYLDFVGEAKALTRVADLAL
                     ININGQSGVEVGTELVFKFAKEDDLPVWFIINMLDKEHADFQKSLNSIRSSFTKKAVQ
                     MQIPVNSGPNFDTIADVITQKVIKYKNDQKGTYTELEPTDEIKERLIALKNELIESVA
                     ESDDVLLEKYLSEGTISEEEFLNGLRNAIIKRNLFPVFVAAGLNNIGVTKIMDHIVNF
                     APTPSAKISVSGSYNGTPITRKISDSEPTSLFIFKTISEMHVGELSIFKVISGSLKSG
                     VDLLNSNNGKIERIGQIYCVNGKEKQEVAHLHAGDIGAVVKLRFSKTSDTLCDQRAPI
                     TYPGISFPEPVIQIAIEPKSRGDEEKISAGLQSLTSEDPTMSWHYDPELKQTILSGQG
                     ELHLAIILQRLKQKFGVDVNQLPPRIPYRETIKKRGDSKYRHKKQTGGAGQFAEVWMY
                     VEPGPPGSGVEFTNTLVGQNVDRVFVPSVEKGVRSACEEGILAGYRVTDVKAVFYDGK
                     MHPVDSKDIAFQIAGKGAFKECFMNGNPVLLEPIYNLEVIVPEEYMGDVMGDISVRRG
                     KIQGVDSEGPFQKITAKVPLAELYRYSTALRSMTQGRGMHRQSFSHYEQMPKEIQDKV
                     VAASRKEHEE"
     gene            22000..22485
                     /locus_tag="PKX09_00455"
     CDS             22000..22485
                     /locus_tag="PKX09_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOU16340.1"
                     /translation="MEFDKLWAPWRIGYITNPKCEGCFLCRYPSESDDKKNLILQRGK
                     SAFVIMNFYPYNNGHLMVAPYQHTNDFAALDDATHLELFHLVEKSVGILRETLKAEGF
                     NIGVNLGTVAGAGLKDHLHIHIVPRWTGDTNFMPVIGHTKVISEGLTETWQKLKSKFD
                     E"
     gene            complement(22469..>22683)
                     /locus_tag="PKX09_00460"
     CDS             complement(22469..>22683)
                     /locus_tag="PKX09_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16341.1"
                     /translation="TATRPAVAGLLQKIHLRKNRIEVAVKSLKRRDSNPHPAWAGLLQ
                     IHNLKKQYKFKSFNSDIQLSKIIHRI"
     assembly_gap    22684..22783
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23013..23084
                     /locus_tag="PKX09_00465"
     tRNA            23013..23084
                     /locus_tag="PKX09_00465"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23045..23047,aa:Asn,seq:gtt)
     gene            23096..23356
                     /locus_tag="PKX09_00470"
     CDS             23096..23356
                     /locus_tag="PKX09_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOU16342.1"
                     /translation="MEKYFLYILFSNNLNHYYVGISSNPEKRLQYHNSQNKGWTKRGR
                     PWQLIFQKEFESKSVAMFWESWIKKQKKRTIIEKIINNSFNW"
     gene            complement(23607..24833)
                     /gene="manA"
                     /locus_tag="PKX09_00475"
     CDS             complement(23607..24833)
                     /gene="manA"
                     /locus_tag="PKX09_00475"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HOU16343.1"
                     /translation="MENNFEIKPFVLRNKIQHYEWGTRGEQAFIPRLLGIQSEGNRPY
                     AELWIGAHPSAPSQVNISNDLIDLTELIKKHPVEILGETVFKRFGANLPFLFKVLSAG
                     KPLSIQAHPNKSQAVRLHRRDSIHYPDSNHKLEIAIAIDNLKALVGLLNIVELRKKLE
                     LYPPIQSFTDRVLPGDSTISEADETTIVKQFYSTLIRKSVSEPQLYGNLVESLAESIL
                     TRGNRISEQEQLFLELKKCYPSSDIGLILILLMNLVQLTSGQALNISAGIPHAYISGN
                     IIECMTNSDNVVRIGLTNKYKDVAALLEILDFSPNEVQVVNPDWRDGISIYRSSAEEF
                     VVANMKVAENFAKKIYTNDKVQIGLILNGRIELIWNSAEKLILKKGESVLLPACIKEY
                     VIRSQNWAEVYIASVN"
     gene            25066..25683
                     /locus_tag="PKX09_00480"
     CDS             25066..25683
                     /locus_tag="PKX09_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOU16344.1"
                     /translation="MNNEAIKISVIVVIAAYISAQMLSDITSLKIISLLGFSIDAGTL
                     IYPFTFTMRDLVHKTIGLKGTRIVIICAAVINLLMALLFWIVSYIPGDPNVGPQLEFA
                     KVLSPVWRIVIASIIAEVIAELLDTEIYRIWVNKVTKRHQWARVLVSNSLSIPIDSLI
                     FVWGAFGGIYNPSVVWSIFFANLILKGAITIISLPMIYLVKDKNY"
     gene            complement(26228..26422)
                     /locus_tag="PKX09_00485"
     CDS             complement(26228..26422)
                     /locus_tag="PKX09_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16345.1"
                     /translation="MCQKWACCVFYYGGILSMLLGILEKLFGFMIFDLKPSRFLMFAA
                     VCALYAIVSIMCQPVCGKKE"
     gene            complement(26464..26661)
                     /locus_tag="PKX09_00490"
     CDS             complement(26464..26661)
                     /locus_tag="PKX09_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOU16346.1"
                     /translation="MWSVYVIRSLKDGRYYVGMSEDVSQRLKTHNSGKVTSTKSRRPF
                     ELATGNKILVNEYFTNYILTS"
     gene            complement(26665..26737)
                     /locus_tag="PKX09_00495"
     tRNA            complement(26665..26737)
                     /locus_tag="PKX09_00495"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26701..26703),aa:Glu,seq:ctc)
     gene            complement(26768..26838)
                     /locus_tag="PKX09_00500"
     tRNA            complement(26768..26838)
                     /locus_tag="PKX09_00500"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26804..26806),aa:Gln,seq:ctg)
     gene            complement(26895..27836)
                     /gene="menA"
                     /locus_tag="PKX09_00505"
     CDS             complement(26895..27836)
                     /gene="menA"
                     /locus_tag="PKX09_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HOU16347.1"
                     /translation="MSANRKLSDSLKLWLRVYRAPFLTASVAPVILGTSVGWYETGRI
                     NFISGLLALLGTIFAHFGANIFNDYYDHLTGCDDNNLDFTPFSGGSRTIQNGLLTARQ
                     AFWAGLVAFLLVVLIGLLLIVYTGRPALIIFGIVGLAIGFLYTAIPVKLAYRGLGEIG
                     IFIAFGPLIVIGASYVQTGTISFLAFLASVPAGLLVMLILYANEILDEKADRSADKNT
                     IIVKIANRNTALKIYRLILYVVPIWIIVFVIVGYFPYWSLIILCLMPKYIGTIFLSKK
                     PFNDVKEMVPLSANTIQLQFLMTITLAISFILAGLTG"
     gene            complement(27852..30005)
                     /locus_tag="PKX09_00510"
     CDS             complement(27852..30005)
                     /locus_tag="PKX09_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOU16348.1"
                     /translation="MRIFRICLCFLLSTSVILAENSKKLTVREIIDDPGLDARTYRQK
                     QFTWTPKGDGVLFLEKNKNQNCDLLKLSINSLKIDTVFTYEQLRWSNDTHTVQLDPFG
                     FISAPDGFRYLLTGENDLFLGDERDGTIVRLTNDGTQKSVPKFSPNGKWVSYICDHNI
                     WIVNCESKSTIRLTTDGSESIFNGLPDWVYAEEFDSEQAYQWSPDSKMIAYCQLNENG
                     VSRYPLVDWSPMHPEIEWQYYPNAGEKIPEVKVFTVDIETQKIVCMNNFQSKNEYVVR
                     IDWLADTSLVVIQSINRLQNCQKVTYGNPRTGQTKVILEEKDPYWLNITDLYYFMKNS
                     RNFIYYSEIDNFMHLYLYDFEGNLIKPITSGDWMVTELNGVNEKDSLVYFTATKNSVL
                     ERHIYSVHIGSGAISQIDTGDGVHSAVFSPDLKYYLDFFSNVNSQVDVRIIQNNGRKI
                     TDLYHNKDFSPQKYNFGVTRFIKIPAIDGDSLYASLLYPSDFDPQKKYPVIVYVYGGP
                     GVQVVQNRYGGAWTQLLAQQGYLIFSLDNRGSYGRGRYWERRIYQQLGKIELQDQLDG
                     VKYLKSLPYIDSRRIGIWGASYGGYMTLYALTKAPEVFKTGVAISPVTDWKYYDAPYT
                     ERYLGLPINNQNIYKESAPLNYVSQIKANFLLIHGTYDDNVHLQQSLEFIDELISNNV
                     RFELMLYPGRKHGITDREGQIHMYETFLEFLQRNL"
     gene            complement(30007..32874)
                     /locus_tag="PKX09_00515"
     CDS             complement(30007..32874)
                     /locus_tag="PKX09_00515"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOU16349.1"
                     /translation="MDDFLTLAGLNEFIALDVETTGLNPQQDAVIEFAAVKFVNGKPS
                     NTLNFLCNPGFSISPEIEQLTGIKDEMLKDKLPFEQHLDEVLEFIGRIPLVGHNIGFD
                     LGFLKEYLKNFKSQRKRILYNPLYDTLLLAQAFLFFLYNHRLSTIAEFYGLSAKGAHR
                     AENDALNTGLIFLKLIEEVRSYDLETIQTINMVLQDTEDPNKLLYKHAAEDLLLKKTL
                     GSAEAPQIGWEPPVNIFGQSIEKNKESAKASLKIEKFFEPAGPIAQNFVGFEDRPQQK
                     EMAQLVLNSLQAGDSAIIEAGTGVGKSLAYLIPAVVWLNQQKKEKKRVVIASNTKTLQ
                     EQIFYKEIPFVSEKLKLPFKAVLLKGRSNYICLTRWHRYLNALPQQLHLAARSAIIPI
                     IIWLKHTRTGDIAENNGFNLAAHYYIWNEICSEPGYCTTSVCQKYGGCFLGKIRYHAM
                     NADIMVVNHSLLLADSAADNQILPGFDALIIDEAHNLEKNSYNYFAQKVNLPTLIYFL
                     NGIYSGSAPERGLLVDLAESAHQLKSLDKIEPLRQMILEKTSDVKIAAEVFFRKIVAT
                     KFNPANGNQKSFGIKKRYKDFSREFPNLEIETNNLIYELGTLWNLLGNLSNKMDEIWG
                     DYAEEYDELQVRLMNQKIMVEAFLETLRKVTTANDDSHIFWYEIAPNGKNNTVEIVCT
                     PLNIANDIFEKVYKNVSSAMLTSATLQVAGAFDYVLSRTGLNLIEDHNVVSRALGSPF
                     YYNDQMAFFTYHQNDNEISEAFIAARLLKRLSSETGKGILVLFTSYNFMYEVYQLCLP
                     TFIEMGVTLLAQGQGASRTALLEQFRLEQNSVLLGTDSFWEGVDVVGPALEILVITKL
                     PFPVPSEPIIEANVEKLKAEGQDPFNEYYVPESVLKFRQGVGRLIRSINDVGVVINLD
                     SRIDKKAYGRYFKKSLPVEPISIIGEDELLAAVRKFFRIGK"
     gene            complement(32874..34052)
                     /locus_tag="PKX09_00520"
     CDS             complement(32874..34052)
                     /locus_tag="PKX09_00520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOU16350.1"
                     /translation="MRKEYDVIVVGGGPAGSTAARFAKTATNTVALFERDREIGIPVR
                     CAEATSIPDLERFVKIDPRWVATTITKARLISPSDQKVWLNLPNDGVILNRRLFDNGL
                     AEIAATQGVEIFTKANVYKIVSDGEQNWEVFVRHLDRDYIVKSKIIIAADGVESRIAR
                     FAGIRTQTVLKDIESCAQVLLGNLDVAENQIDFYFSSRWAPGGYAWVFPKGNHTANVG
                     LGVNGAYYKGRSAIEYLDDFINEKFPEAARLTTVAGGVPVAKSLKKIASDGLLIVGDA
                     ARQVNPVTGGGILAAITAGKIAGLVAAQAIARNDWSVNVLKEYQTEWDKNVGKDYARF
                     YKIKEWMTELSDDELDEIATLLQKIPPDDINIMTVFKIALRNKPALLLEAIKLFAQMI
                     "
     gene            complement(34061..34234)
                     /locus_tag="PKX09_00525"
     CDS             complement(34061..34234)
                     /locus_tag="PKX09_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU16351.1"
                     /translation="MIEVNPDCCDLCGTCISVCPKDCITLTESRLIVDDSQCNVCQIC
                     VRVCPFKALRLVE"
     gene            complement(34231..35196)
                     /locus_tag="PKX09_00530"
     CDS             complement(34231..35196)
                     /locus_tag="PKX09_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOU16352.1"
                     /translation="MLKVPSKSIAWLDYLFIFRPTLFFAVWIITLAGYSGYFIFSVKQ
                     PAWWSLEVNWKLVLGFIVVTMATGATFILNQLHDIESDKENRKLFLISEEHIKADLAL
                     KIALITIGVSLVLALIINWQFFIALGICVFFWGYLYNYKPFEWKDKPMLGVLVNFISG
                     LCLFICGWIIAGKWQLKDIIYAIPYLLAWTAVAVLTTIPDKEGDLKTGKKTISVHYEN
                     RVTIWLAFICLALSFALGMYFKDPVISLSCLISSPLFIILLFKPTEAWVLRSIRYPIL
                     FLALTLCVEFPLFFIVLLINYYLCRFYYLSRFNLNYPTFQVEESE"
     gene            complement(35190..36554)
                     /gene="purF"
                     /locus_tag="PKX09_00535"
     CDS             complement(35190..36554)
                     /gene="purF"
                     /locus_tag="PKX09_00535"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOU16353.1"
                     /translation="MCGIVGIYNHEEAATLTYLGLYALQHRGQEGAGIASFDGKVSHL
                     QKGKGLVADVFINREQLMHLSGKTAIGHVRYSTYGSNGNENIQPLAFKYKGSQISIVH
                     NGNLINLDEIGRDLESHGALFQTTSDTEYIIHLFAHSGGDSLVEKFLQVFRRIKGAFS
                     VLILFDDKMIVARDHRGFRPLCIGTLDGSYIFASETCALDLIGATYERDVKPGEMIVL
                     DGNNVKSYQIAPPEPKHCIFEYVYFSRPDSQIFGEYVDKTRRKLGKILAMEKPAPEAE
                     IVISVPDSSNTIALGYSQRSTARFEIALIRNHYIGRTFIHPTQTMRDFTAKIKFNPVG
                     GVLKDRKIVVVDDSIVRGTTLRKLARMLRSAGAKEIHIRIGSPPVKFPCYYGMDFPSH
                     EELIANQKSIAEIKDYLEVDSLEYISPEGLLNAMSLPAENFCTACFTGHYCEKINQEK
                     ENSC"
     gene            complement(36558..37607)
                     /locus_tag="PKX09_00540"
     CDS             complement(36558..37607)
                     /locus_tag="PKX09_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOU16354.1"
                     /translation="MKTLNEYIGCIHIHTVYSDGSGTYEEVIRAAQEAGLDYIMVSDH
                     QTLRGRDEGYAGWHDNLFVSIGYEIQDLEDRHHYLVFGMDKTLPTDLTHQQYIKAVKE
                     AGALGIAAHPLEQRDKNYSLPGFPAITWSTLDYPEIEVVEIWNMMSHWLEATTIKNKY
                     WNVIHPRSFSTSPADELLKWWDNINLKRNVVGIGSVDAHATKVKVFGVFPKAIFDYKI
                     MFKSIRTHLLSERKITELNDSDTEAEIIKIIKSGRCFYSNFRRGDARGFRFYVQTDQT
                     ELQMGDTGMATGGTLYVFLPQKAECKVYRNGKIYHTETTTKLAIDIPEGIYRIEAKRN
                     QRGWIYSNHIKLING"
     gene            complement(37604..37963)
                     /locus_tag="PKX09_00545"
     CDS             complement(37604..37963)
                     /locus_tag="PKX09_00545"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HOU16355.1"
                     /translation="MKNWWLLLLIFAIGGRLLAAPPVGEIIKSEPDDKWFAMDKLKHF
                     STSFYMSTSLFYTQNRIFDLKTDRANRNAAGLTISLGLLKEIRDLKQKENYFSWRDLV
                     ADILGAGAAILFINGIE"
     gene            complement(37960..39315)
                     /locus_tag="PKX09_00550"
     CDS             complement(37960..39315)
                     /locus_tag="PKX09_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOU16356.1"
                     /translation="MIKSREQWGSKIGFVLAAAGSAVGLGNIWRFPYLVGENGGAVFI
                     FIYLICVAVIGLPVIISEVVIGRNAQKDPVGSFKKLSNNNPFWKGVGFLGVFTGFIIL
                     SYYNVIAGWCLGYIVEAVKGSFQTITDTAQAVALFSRLSGSGSWSVPYLTIFMALTVG
                     IVYFGVTRGIEKSSKIMMPSLIIIILILVVRGITLENSKAGILYLLKPNFANVTGRTV
                     LVALGQAFFSLSIGMGVMITYGSYLSKENNIPSQATRIVVIDTSVSLLAGFMIFPAIF
                     ALGLKPHQGPSLLFNTSPILFNYIPGGYWFRILFFILLALAALTSTISLLEVITAYFV
                     DEFKLNRKMIAIIGGAISLLLGIPSALSFGRWSSFQIMNLSIFNFCDYLASNICLPIG
                     GLFITIFVGWFWGKNNALLEMRQGAEGTPAWFFPVWRFLIRYVAPVLIAMVLLSSLGI
                     I"
     gene            complement(39317..39754)
                     /locus_tag="PKX09_00555"
     CDS             complement(39317..39754)
                     /locus_tag="PKX09_00555"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOU16357.1"
                     /translation="MKISPKGAFIRSAIIPGWGQVYNKKPVKGLVLLSAEGYFLYNFF
                     YYNQIYDYVKTTKETLGIETWVGLTEEEKKAQVLAVTGYELTLNSWRPREKRNKYGWW
                     SLGTYIFCLLDAVVDAHLINFPKGEIELGSTEIGLNLSVRVNL"
     gene            complement(39854..40738)
                     /locus_tag="PKX09_00560"
     CDS             complement(39854..40738)
                     /locus_tag="PKX09_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOU16358.1"
                     /translation="MPYKFAVIVNPVAGKGKTIHNLPLLKEIAAKTKSRFDYFITEGP
                     LHATKIADRIHQEYDAVVAYGGDGTANEIMNGLAGTETPIGVIPEGTGNDFARCINVP
                     HKFERAIQILDEYKTRLMDLGTIGNRVFINGVGVGFDAYVNVKTKSLKMLKGSMAYLY
                     SVLSSLAFWKSLPLAIEIDGCPISTDRSFLVAVGNGWACGGGLKLNPLASIHDSLLDV
                     CLIPEMPVWKLFLNLRRLKDGTISEVPGVLTMRCQAINISSEKPLPVHFDGEIFDAGA
                     GEVDIKIVPQSASVVGAW"
     gene            complement(40739..41140)
                     /locus_tag="PKX09_00565"
     CDS             complement(40739..41140)
                     /locus_tag="PKX09_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOU16359.1"
                     /translation="MLGEVERQIVNTTDAPRAIGPYSQAVISGNFVFTAGQLGLDPVA
                     GKIVSNDFEQQVRQIIRNLKAILQAAGSDLKNVVKFTVFVTDLDNFGLLNQVFGEYFP
                     ESAPARSAVQVSRLPLDGLVEIEAIAVKRGK"
     gene            complement(41125..42570)
                     /gene="gcvPB"
                     /locus_tag="PKX09_00570"
     CDS             complement(41125..42570)
                     /gene="gcvPB"
                     /locus_tag="PKX09_00570"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOU16360.1"
                     /translation="MEKLIYEYSQSGKEGVSLPQGFESAVSPAELIPAELLRQSELQL
                     PEVSEPEVVRHFTNLSTMNHHVDKDLYPLGSCTMKYNPKVNDRSAALSGLANCHPLQD
                     VGTVQGALQIIYELERYLCAITGLQAATLQPAAGSHGELTGVLLMKKYFDQKGENRQF
                     ILIPDSAHGTNPASAASANYKTISLKSNQRGCIDLGDLRAKMNFSVAGLMLTNPNTLG
                     LFEEEVGEINEIVHNQGGLVYMDGANLNALLGIVRPGDMGFDITHINLHKTFATPHGG
                     GGPGSGAIAVNEKLVEFLPVPRIEKNGDRFSLNYSYPRTIGKVLGFYGNFGVIVRAYC
                     YIRMMGTKGLQAVSRNAILNANYLRQKLSSVFEIPYNRICMHEFVASGERQLQRSGIK
                     TLDIAKRLLDYGFHSPTVYFPLIVKEALMIEPTETESKETLDRFIEALVKIDQEATNE
                     PEKLRNAPWTTPVRRLDELKANKELNICWGK"
     gene            complement(42583..45204)
                     /gene="alaS"
                     /locus_tag="PKX09_00575"
     CDS             complement(42583..45204)
                     /gene="alaS"
                     /locus_tag="PKX09_00575"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOU16361.1"
                     /translation="MKTANDVRREFLEFFASRGHKIIRSAPVIPQSDPTLLFTNAGMN
                     QFKGIFLGQEKIESPRVANSQKCIRVSGKHNDLEEVGKDTYHHTFFEMLGNWSFGDYY
                     KAEAVQMAWELLTEVWKLPTERLWVTIYQDDEEALQAWQRIAGLDQSRILRFGEKDNF
                     WEMGETGPCGPCSEIHYYTGPHPEHQQAEMINSGNPEYIELWNLVFIQFDRDKSGELK
                     PLPLKHVDTGAGLERMVAVLQGKKSNYDTDLFKPIIDRIVSLTGKEYHTENGVPHRVI
                     ADHVRMLTFAIADGGLPSNDGRGYVIRRILRRAARFGRMLEMHKPFIYQLVDSVIEVL
                     GATYPEIAERRNHIQAVIKAEEKSFGLTLDRGLEVFAKLKESLQRNNIRIIPGEEVFK
                     LYDTFGFPMDLTRLLAEEAGFSIDEKGFENLMNRQKERARASVVFQADDFLENEDWQT
                     LTTGDDSEFVGYENLETQSVIRKYLIEGQKVHLILDRTPFYAEAGGEVGDVGIIAGNN
                     FRIRIDNTRKIGSSIIHSGHFEKGNLIDNPMVKASVDNVANLQIRANHTATHLLQAAL
                     RQILGEHVHQSGSLVTPEKLRFDFTHFTKMTKSEIEAVEDWVNARIRENLPVNAEVKD
                     YQSARESGAMALFGEKYGDVVRVINVGDCSKELCGGKHVNRTGDIGCFKIISETAIAA
                     GVRRIEAITGESVLKLARENERLLEEITTLLGVSREQLPHRIEKLLTEYKSLEKQFHK
                     NNKADLEQEIDTLLEKAEMIGELKVFTYQANVKSLAELKEIGDLVRAKLHNGIGVIAG
                     VIDEKPSIVVVVSDLAIKQYGLKAGDIVRELGKILGGGGGGQAHLATAGGRFTDKIPE
                     ALQAVIPLIKSKIESKR"
     gene            complement(45350..46003)
                     /locus_tag="PKX09_00580"
     CDS             complement(45350..46003)
                     /locus_tag="PKX09_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOU16362.1"
                     /translation="MSSDFLSEAEILKVVTKIESQKKKSARINIYLDNEFAFGISAET
                     LNLFRICVGQQLTEKQINMIQQHELFEQAKEAAIRYLGLRIRSQRELELYLTRRQKYP
                     LMVASRVIKYCTERGYLDDKVFCETFIRDQLELNHNGIRKIRQALVAKGIDREVIDEA
                     IKNLVKDEDQLKAAWIIGQKKASTLKNDPKRRDKLYRYLIQKGYKPDVVIKVLQKIL"
     gene            complement(46031..47038)
                     /gene="recA"
                     /locus_tag="PKX09_00585"
     CDS             complement(46031..47038)
                     /gene="recA"
                     /locus_tag="PKX09_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOU16363.1"
                     /translation="MAEKNDDKIKALDLAITQIDRQFGKGSIMRLGDDSMKITVDVIP
                     TGCLSLDACIGVGGIPRGRITEIFGPESSGKTTLALHIIAQAQKAGGYAAFIDAEHAL
                     DPQYAAKLGVNTRDLLISQPDHGEQALEITETLVRSNAIDVIVIDSVAALVPRAELEG
                     EMGDSHVGLQARLMSQALRKLTGAVSKSNTAVIFINQIREKIGVTYGNPETTTGGRAL
                     KFYTSVRLEIRRISTIKEGNTNVGSRTRVKVVKNKMAPPFKETEFDLTFDQGISYVGD
                     ILDTAVEAGFVQKSGAWYSYGDERIGQGRESAKGYLTENKELLTKLEQQVKTYLGLIK
                     E"
     gene            complement(47054..47611)
                     /gene="thpR"
                     /locus_tag="PKX09_00590"
     CDS             complement(47054..47611)
                     /gene="thpR"
                     /locus_tag="PKX09_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOU16364.1"
                     /translation="MKRTFIAIPVSQETCLQVDTIQRTIPELRENTRLVPTNNLHLTL
                     KFLGDTDEKVIPTISQALDSSITTIDRFEFIWEGLGCFPNNHKPRVLWLGVGQGLEQI
                     QKLTRAIELVLENFGYQPEKREFRPHLTIGRVKNPFKKVAGLESFLNYKINPTINPVK
                     DVIFYESNLTVGGPIYTRLAVFNLK"
     gene            complement(47615..48844)
                     /locus_tag="PKX09_00595"
     CDS             complement(47615..48844)
                     /locus_tag="PKX09_00595"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOU16365.1"
                     /translation="MTNAAIISIGNELISGLVNNTNSAYIARTLNNFGIVVSLIVTVG
                     DEQKHIWTVLDNLPPEINLVMITGGLGPTNDDITVSALARYFNLPLVFNKETASNLVK
                     HFAKRGLKMPEINLKQAYLPKEAVILDNPVGSAPGLKIEHQNRVYYVLPGVPREVEAI
                     MTKIIASQLAALGHKAIVKKYLHITGIPESQIYTLLKEWIEQHPHVKVAFLPQTAHID
                     LELTISEPQESAKIDEYVKEIAEILGDYVFGFDDDTLESVIANLLLQKHLMVAVAESC
                     TGGLIASRLTDVPGSSNYFKTGIVAYSNATKINLIGVKPEIIEKYGAVSSEVANQMAA
                     RVRQISGCDLGLSSTGIAGPSGGSDAKPVGLVWIGVSDKNATRTFQYQFSGDRLTNKL
                     LFSQAALNQLRLYLIGK"
     gene            complement(48841..49320)
                     /locus_tag="PKX09_00600"
     CDS             complement(48841..49320)
                     /locus_tag="PKX09_00600"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOU16366.1"
                     /translation="MNITGAKKLLSRIIASVCFIGYLPFAPGTWGTVAGLVVWFLLPW
                     HSFWFDLILVTVTFFIGIFTSEYIEKQIGEMDPAYIVIDEVVGVWLALAIIPTLTYPK
                     DLALIVLVFLIFRIFDIKKFKPIRKLELIGGGFGIMADDILAGIFTGIIVNLGILIL"
     gene            complement(49317..49916)
                     /gene="pgsA"
                     /locus_tag="PKX09_00605"
     CDS             complement(49317..49916)
                     /gene="pgsA"
                     /locus_tag="PKX09_00605"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOU16367.1"
                     /translation="MKKLFNWPNLLTFFRILLTPLFLYFLFGNQPYFEIFALLIFIIA
                     SVTDIYDGYIARKYASVTKLGKFLDPLADKILMSAAFISFVILDLVPLWMVVLVILRD
                     FIVTGIRILMNSRHLTMETRKSAKTKTGVQVGVVCFILAYLITQRWEIFLGISDYVRL
                     IEEYRIIYILMLITTIITIWTGVEYILVNKDKIQQALKK"
     gene            complement(49918..50511)
                     /gene="recR"
                     /locus_tag="PKX09_00610"
     CDS             complement(49918..50511)
                     /gene="recR"
                     /locus_tag="PKX09_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOU16368.1"
                     /translation="MSALPDSLQNLIERFARFPGIGRKSAQRLAFYLLKADREEAVAL
                     AKAIIDVKDRIYKCSQCYNISETDPCKICTDEKRDHTTICVVQEPMDVFSIEKTGHYR
                     GLYHVLGGVLSPLNGIGPDDLNIQGLLYRLANVKEVILAINLTRDGETTALYLSKLLK
                     SKNVKVSRIAQGLPAGIELEYADEITLTHALEGRTSF"
     gene            complement(50508..50843)
                     /locus_tag="PKX09_00615"
     CDS             complement(50508..50843)
                     /locus_tag="PKX09_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018936735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOU16369.1"
                     /translation="MPKGGLNNLMKQFSKVQAEMERVQEELANLTVEGSAGGGMVKVV
                     ANGKQEIQSIKIDPEILKDDVEMVEDMIVAAVNQALTRAQELSQEKMAGLTGGMLSNL
                     PGGMKIPGL"
     gene            complement(50856..52508)
                     /gene="dnaX"
                     /locus_tag="PKX09_00620"
     CDS             complement(50856..52508)
                     /gene="dnaX"
                     /locus_tag="PKX09_00620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOU16370.1"
                     /translation="MNYKVISRKYRPQNFNEIIGQDHICTTLQNALKSGRIAHAYLFT
                     GSRGIGKTSTARILAKALNCLNPQDYNPCNVCQNCVEITNSRSLDVIEIDGASNRGID
                     QVRELRENVKYPPSNSKYRIFIIDEVHMLTREAFNALLKTLEEPPPHVVFIFATTEPL
                     KVPPTIISRCQRYDFHRIPVREIVGQLKKIAESENLTVSDDILTLLAKKSEGAMRDAE
                     SLLEQVVSFSSDNVNLNSVQKLLGLTDYEYYFTISNQVFEHDLNALIKSAEEVFNNGI
                     NISEFLIGLSEHFRNLLITAIVKSAADLDLPDSLKNHYQQEAQRWSTNDLMRLLKLVG
                     EAQTGLKAAMNQRTHLEFALLRMGAMEKTVTIQSILQNIDTRHSGEMEQIKPVAQTMD
                     FFSTKPVVSKTAPHPDSNVIKEKVSDSYIPEKKSEPIPKSFDSLVTFEAIQSQWSSII
                     LKLEESNLSLAEFLKVSKPMQLNGNVLDLGFTSEQDFARKNVAKRAPTIEQTLIGFFH
                     KPIKIRCIQIESAAESADTVKKPIDEMTEKVVDMFDGEIIIT"
     gene            complement(52520..52786)
                     /gene="ffs"
                     /locus_tag="PKX09_00625"
     ncRNA           complement(52520..52786)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKX09_00625"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(52816..52888)
                     /locus_tag="PKX09_00630"
     tRNA            complement(52816..52888)
                     /locus_tag="PKX09_00630"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52853..52855),aa:Ala,seq:cgc)
     gene            complement(53108..53578)
                     /locus_tag="PKX09_00635"
     CDS             complement(53108..53578)
                     /locus_tag="PKX09_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16371.1"
                     /translation="MANYYTLNKNLSTRRTTSPTEETPPALEVKIDFSATEQRYEYIR
                     SKLDDIMENIGQCYSERLTKELLHRLEKTILDFHTEVIALLDKLEKLEIERNQLPERT
                     PVSESVSTPVESKSGERAKRIEESEQRKAGIVIESELNKKTESEPKRRFFNRRK"
     gene            complement(53557..54462)
                     /locus_tag="PKX09_00640"
     CDS             complement(53557..54462)
                     /locus_tag="PKX09_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16372.1"
                     /translation="MKSESAKPESPHLMWGLPGRGNLAFIMSLLISLFINFRAGNSQE
                     ILVIHPNVGATIDLEEKIYYDLFPEFYNFSDAQFYQTSSDTVIAKIRLWIDNGYQEQL
                     LYFTPYQMYLIASRISVREPLTDENRQLIQKRYKPLYAEKFLAEIPMNSYCRLKLKDK
                     SQFDAVYYRLKGDSLLFWIDRQVVPINRNDLVKLKYWDNYRPNNWVKWGTMGSVAVIS
                     YFGSGLVADWLNITTKNAILTQFAAASVGCVIGYKISPIVNEYIMPSTVIKFPTGNIK
                     RLDTIARMCYNIRNIKDKIWQIITP"
     gene            complement(54476..56641)
                     /locus_tag="PKX09_00645"
     CDS             complement(54476..56641)
                     /locus_tag="PKX09_00645"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOU16373.1"
                     /translation="MKKYIVSLAFLLLCNLAIAQNQMATVLKVAGDVKLRTKGRSDFS
                     RKVTMGMSINTGDALKTGSDGFVAIIFTEDKSLLKIRNDSEIELIKDNLGDRTSKVTQ
                     GKVLAEITPGIKTSFRIETPTSVASVKGTKFWTITIPDFGDKFYGVEGQVGILNLITG
                     LEADLEPGKMVVSTIDGELFSLPVDPDEIPADPEEMKPEEEPGKPSGMKMEEETEMPA
                     MGAIEATGQPPAESVAEAGKKEEEAQKAKPFGMGLGLGSVTIGGKIYNQIALRPEFKF
                     GKLGVSLDIAFYMDENGNIRKEEWDEFTDYFDKIYYVRWAQQGDPFFIKVGALDNVSM
                     GYGILLNGYSNTTEYPQVRKVGVHTGMQLSKIGWEAYMANLKEITGPGLMAGRLTYRP
                     ISKFPLVLGTTVVADVYPYKGLPDADEDDVADALDLYPEADDNVVLDSLKTSFTLGQR
                     EFLRNNGFNIPSETIIQNGITKLSDYDRLLNGAVSVDVGVPILNKKSFNLTVYGQAAS
                     FFPVEDSAVVYNSATQSYDRVAFTPGYGFAVPGVKMGIARIANLTLEYRYAAENFLFN
                     YWDRAYDYERVQIRNNQIYTKQQMSLMQNSMQGIYGAIDVNIMNYFILGGYYQHMFSE
                     GTEIKSFMASASIPKGIVPKLADATAFYQRNNDENPFDFKNPSENTILGYRIGFELGG
                     GAIIYYKFQRTYRDFDGNGIVDPDLEAVNLTTIETGFSF"
     gene            complement(56665..57900)
                     /locus_tag="PKX09_00650"
     CDS             complement(56665..57900)
                     /locus_tag="PKX09_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOU16374.1"
                     /translation="MDTAILFLPERMTSNKANSIFNTIRGILHNQLNLNIVIQNLQNS
                     PPIYEQITSIKNALVFVSIYDSIYLEKFENFQPLFKIKTKSSDNILVIPQKATVRHLK
                     ELAGGKVLITKGIDHNCVNPVILYLIKNCGIFFAPKSLSIYYEPKTLLDQLLKGDVSA
                     ILTNTFQFDLWEADEQQSLKILGKCPEESEIVALIGPKFNVRKLKKNFNDWLQEHGSD
                     YFKDGYKLQSIDKYENILLIEAIEGLGYKLKDFIENYPDLIIKNIKSKLAEEVETLNE
                     KYSGLQVFNEKLVDMYKEIRESHDRLYQEIDSAVENQILFLKDGTIIGISRGFMNWVQ
                     KSRPEIIGKNINDFINPNINKPFNELIQQVDYGLIKSFGIRLKNTNDNDTTAKMDFTI
                     LELRDSKVVMGIVSMITDR"
     gene            complement(57911..58279)
                     /locus_tag="PKX09_00655"
     CDS             complement(57911..58279)
                     /locus_tag="PKX09_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU16375.1"
                     /translation="MNQYKILIVDDEQNVCEFLGEFLQDKGYLVIKARSGSKALRYLG
                     KNNPDLVLLDILMPGMSGLEVLKRIRKLYPDLPVIILTGVKDKQVVDDIINVGPVDFI
                     PKPIDLDVLEKCISSNLAKK"
     gene            complement(58427..59689)
                     /locus_tag="PKX09_00660"
     CDS             complement(58427..59689)
                     /locus_tag="PKX09_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16376.1"
                     /translation="MKNGWQIAYFLMLSFLLFGLVGAQTPNSHPINLFILTFTNYEGA
                     PEIDWWKEGFVDFLMDYFSNNPDLNVHRINDPATIPNQIKNLSETEITRCYLLTGNYK
                     IIDQKFEVDLELIDINTWRLLQKKNISIQSADMAKIIESVNHGATEVLTPYLIKKAET
                     TVAQEISSPSQPTKNNYQKAVAASVAISSVIDKFSETHPESAQHEPSFGGTVTNELSL
                     KFGEAIAQTGSFQYTINQIIRNPYKLEISDPLFRRLSANDEIINVTFFINYVLSRDLI
                     QDMLTTFPYEIQTKNDEYTEYIFSGKRFAYSEDFIKAIARGDYRTHPVYAFITNTETV
                     ACVLIDMPIMYRKNLISSSKVQFTAKYQPLINVTANGWNVAIQLKNTDAKAEYNFDIS
                     FEQLKNLSRIDVMFLSEADIQEYLKIIN"
     gene            complement(59699..61138)
                     /gene="gltX"
                     /locus_tag="PKX09_00665"
     CDS             complement(59699..61138)
                     /gene="gltX"
                     /locus_tag="PKX09_00665"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOU16377.1"
                     /translation="MTENTRVRFAPSPTGSLHLGGARTAIYNWLFARSVGGKFLLRIE
                     DTDVERSRKELVNQICDSLQWLGLNWDEEPVSQSQRLELYQQKVTELLKAGKAYRCFC
                     SSEELEAEKAATIKQNKAYRYSGKCRSLSLQQSEELMAQNKPYTVRIKLEPGVIEWDD
                     LVYGHIAVNNNELDDFIIQRSNGVPVYQMAVVVDDIDMRITHIIRGEDHIPNTPKQIN
                     LFRAFGQPIPQFAHLPLLLGPDNKRLSKRHGATGVDEYREQGFPAEAVVNYLALLGWA
                     PQNDQEVLSIDEIIQQFQLTAISRKPAVFDIKKLEWISGEHLNRMPVAKIRSVLVPKL
                     IAAGLINQQESENRTQYVDSVIELLRGRVRTYNQYLEWGCYFFQDPENYDEKAAAKYW
                     HTPETNEHLAKLSQELATLTDFSANTIEETLRRTAEHAGLKAADLIHPLRLALTGFGI
                     SPGIFQVAEILGKETVLRRIQKAIEYFKT"
     gene            61406..62371
                     /locus_tag="PKX09_00670"
     CDS             61406..62371
                     /locus_tag="PKX09_00670"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOU16378.1"
                     /translation="MQNISKYRILQNILLLFSLASFVFADEVWLLINPETRYASLAGA
                     ATAIVNDVAALYYNPAGLANCDELSASANYGTWLGNSEIYYAGAVRNFKHRGGLGTSL
                     YLYNLDAKITGDDYTATDFSYNFIYVSIGYGCNFTRKITLGAATKFIYTNAPNTASDG
                     IQSINGKGLACDLGIQINNLLPFLTIGKNKTGGLSLGAAFCNLGPAIKYRNDFTNYKS
                     KLVPRLRLGGGWRLLNKDLISATISYDFIEDYYDSNSSNHLKSHLIGGAFSFYKRLYI
                     HCGQHIENNENYITWGVAYNLDKFIIGYCRYPSQKSNFFSVSYSL"
     gene            complement(62468..62911)
                     /locus_tag="PKX09_00675"
     CDS             complement(62468..62911)
                     /locus_tag="PKX09_00675"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HOU16379.1"
                     /translation="MKSKFWDWLELFIRLVIGFLFIYAAVGKIIHPEKFAVIVYNYRV
                     LPVELVNITAILVPWLEIALGVTLILGIWLETAAFLLSILIIVFISLIISAIIRGLNI
                     ECGCFTLSKTGSLVSWKRVAEDLLILAGSLFIFFRHLPQEQSTTE"
     gene            complement(62908..63477)
                     /locus_tag="PKX09_00680"
     CDS             complement(62908..63477)
                     /locus_tag="PKX09_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOU16380.1"
                     /translation="MVLSITMKQMVKTILLRSIWLGLGVIVVGLIFNWLSPNGIPLVA
                     KYQTVTVRGKSQKIPLFMNRKVQTNQETDLEISPVSELDTEKAWQFFTNGGTIFIDTR
                     EYEDYLAGHIAGAISIPLSEFQNDPTIVEELDRQVRIVTYCDGSDCQSSIDMAVRLNE
                     MGFEQVWFYFGGWKEWVEADLPIVKGSKP"
     gene            63539..64450
                     /locus_tag="PKX09_00685"
     CDS             63539..64450
                     /locus_tag="PKX09_00685"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HOU16381.1"
                     /translation="MSSKNSIPVIKFLSFTLILCLGGLSCSPQRFLFNKVSNVFDSGI
                     EIIYQEGDLELAERFLSSNLETIVILVAQDPENPRLNLMAAQAFGAYAMAFVEDKDPQ
                     RAGLLYVRGLEYAFKSLPPQIRFSRNITPADLEKLLVESRKSDVPQLFWVGYNWGMYI
                     LQNLDQPRLLGDLAKVELLMRRVAELDAGYNFGGVDIFYGTFYSARPPMLGGNPELGR
                     SYYLKNIERNPNFLMTKLYYARYYAVQVQDKELFDNLLTEIETFDLESAPEIRLFNAL
                     AKQKATNLRQNAELFLDLNEILETNER"
     gene            64458..65468
                     /gene="dctP"
                     /locus_tag="PKX09_00690"
     CDS             64458..65468
                     /gene="dctP"
                     /locus_tag="PKX09_00690"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOU16382.1"
                     /translation="MRMKYINAVILIILCTIPNLVAQKVHTIKVATVAPEGSSWMRQF
                     RQFEKEVDALTNGQVKFRVYASGVQGGEKDILRKMQLGQLDVGTFTGVGLGEIVPEVR
                     VFDLPFLFRNSAEVDYVCDALFDEFQTKFLEKGFFLAGWAEVGFVYLFTNTPVTRLAD
                     FKNVKMWLWKGDPLAKATFDEMKIAAIPLDITDVHTGLQTGLIDGVYISPYGALAMQW
                     HTKTKYMLNYPLTNSIGAILITTKKMNTIPEDLRNILIKKTKENTRQIVLAGRQENLE
                     SIQILKENGMQLTNLKSDAAAEFIEAGVRTRDRLAGVLYSREMLDRVLALLDEYRAEH
                     AE"
     gene            65440..65934
                     /locus_tag="PKX09_00695"
     CDS             65440..65934
                     /locus_tag="PKX09_00695"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOU16383.1"
                     /translation="MNIALSMLNKIRTIIEKFVTALAVVLMLFLVVMAFLQVILRNFF
                     AIGLNVVDELMRNGVLWIAFCGAALTTLRSKHISIDILPRIVKGKARLVLNWILNLTA
                     AAICLCLSWYAWQFLRLEISMNSMIAGVIPAWIIEIILPVGFFLLALTFLLRLVDKSQ
                     NLEN"
     gene            65934..67205
                     /locus_tag="PKX09_00700"
     CDS             65934..67205
                     /locus_tag="PKX09_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOU16384.1"
                     /translation="MALLFLVLIVVLIFCGLPLFAAIGITGIIQFAISGIDPTVVIVE
                     MYRVASAPTLLAIPLFTLAGYVLAESSAPRRLLNLAEALLGWIPGGVAIGSLVTCALF
                     TCFTGASGITIIALGGLILPILLKNNYSEKFALGLITSSGSLGLLFPPSLPLILYGVV
                     ASVSIDQLFIAGLIPGILLIILLSIYAVRNDVLKKSERIPFSWQNLGRALKETVWELP
                     IPVIILGGIYGGFITAVEASAVTAFYVLIAEFILNRDLSFRRDLIRIMRDSVQLVGGI
                     LIILSCSMGATAYLIDAEIPQKALLWAQQVFSSKITFLLVLNIFLLITGMMLDIFSAI
                     IIVVPLIVPIALSYGINPVHLGIIFLTNLEIGYLTPPIGLNLFISSYRFDKSITELYR
                     ISLPFLFIMLIALVLITYIPDLSLFLVGLIQ"
     gene            complement(67379..69754)
                     /locus_tag="PKX09_00705"
     CDS             complement(67379..69754)
                     /locus_tag="PKX09_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU16385.1"
                     /translation="MHQKNLPVFFFILFILILTEANAFGEEHLIFGKLVSETTGQPVE
                     SANILLEDGSGTFSDKDGNFRIKTSFLPIKVTVTHIAFRDTSFSATSEDCGTIYLKPT
                     VITGEYVFVTATRAVKGKTPVAFSELSIEEIKSRYTVEDVPMILAIEPGVYSYSESGN
                     GTGYSYVQIRGFDQSRIAVMLNNVPLNDNESHQVYWVDHGDILSDAADVQIQRGIGNS
                     LYGSSAFGGSINVLTTIGTDRPTFSIDLGGGSYNTSKIRLGASSGQLHNGKLRLSARL
                     SQIESDGYREYHETFQRSGSAGIEYNGQNTSHQFRALVGYQKSNLMWDGVPAELINDR
                     VGRRSGYKSYIDNFLQQVYSLNSTWRISENLNFNNVAYLVKGDGYYESEKSAAYNLAV
                     DDATARDEFRDFLRSFYLDQYYPTDADSQSLELTRRKWIVNSYYGIVPTLTFIKAKYR
                     LDIGGEFKYYNGNHFGEISRFSDQNLIAQIGKGWYRYYRYIGEKSIATGFVHFTLEPL
                     KKLKLITDLQYQHLQWNLDQKKIGYAIGYDISAKWDFLNPRFGAIYELSNDFSIFANY
                     GRSQKEPADEQIIEADDYWSEPQKAAAELIDDYELGANYRGRKLDVDLNLYRINYKNE
                     QLKNIDIEQEGEYVYTQASATIHEGLEWEVRYQFNPLWALTLNGTFSRHVFESGSYKG
                     NTLPNIPGILFNGSLDVKPVKNLSISAIIRYVGEQYIDEANIGMNPAYWLTDVGLRYS
                     FGRVEIGAKVNNLFNRLYSTYGYGYEWGEYYAYYWPGATRNAFFSISYHLD"
     regulatory      complement(69744..69846)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(69909..70001)
                     /locus_tag="PKX09_00710"
     tRNA            complement(69909..70001)
                     /locus_tag="PKX09_00710"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69964..69966),aa:Sec,seq:tca)
     gene            complement(70006..70848)
                     /locus_tag="PKX09_00715"
     CDS             complement(70006..70848)
                     /locus_tag="PKX09_00715"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOU16386.1"
                     /translation="MKRFVFLILIPAFIFAQNIGELKQKIRVGEVDSVRILLPALIAE
                     YPDNPELLYINGVIETDGEAALLIYRDVLSRFPSSSVADDALLKIIEYLFTKGLYTKT
                     EKYASELIKTYPQSDLIDKAIYLRLCSFNALNKRDSVDYYYKYYSTQYPNLNFHFEDY
                     KIASKVTSIESKKQSQPTSSQISSNAKSAETQTYSLPKVEVPGQGNFTLQMGVFGDPA
                     NARALRSKLEKLGYSVTLRQIERSGRKLTAVLAGSFKSEEEARAVGNKLKKDHNLDFI
                     VVKQ"
     gene            complement(70845..71993)
                     /locus_tag="PKX09_00720"
     CDS             complement(70845..71993)
                     /locus_tag="PKX09_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU16387.1"
                     /translation="MSLLDILLTLVLIALIGFIVINLTGRRRLSRSKDSASLYAMALN
                     HMLHGDDDVAIRLFRDVIRADTDNIDAYINLGILMRKNGKPKNAIKIHESLLYRQEIS
                     NIQRLEILRNLVEDYIAAGDKIRALNYVENILSIDKKNIWALEKNHILNRDLGRWEKA
                     SEYLAMALELKKERNDRLLALYKVQEGLVKYQAGEYHEARLIFRKALKIDPNCEAAYY
                     YIGKSYVLDHREEDAVEEWVKFADVAPHKAYLIFKPLQTILFNLGNFGNIESFYENLL
                     RRIPDDPKTLIALADFYEKKGNVKKAIAILEDLVEKNPGLSIAKINLAKLLVGQAELN
                     RATAILNDIIEKMVDQKEYICSNCGNRSSDILWICPVCGLADTYFTDV"
     gene            complement(72006..72332)
                     /locus_tag="PKX09_00725"
     CDS             complement(72006..72332)
                     /locus_tag="PKX09_00725"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOU16388.1"
                     /translation="MKFLRWVVTLLIMFLIVFFVIQNSNLNPPVKIQFFWKASPVEMD
                     ILILVFLTFILGLVIGFLIAGIKILTTKNELRALSNDYKKLKKEVDLLRNQGIDETEA
                     DNSKTE"
     gene            complement(72347..73645)
                     /gene="miaB"
                     /locus_tag="PKX09_00730"
     CDS             complement(72347..73645)
                     /gene="miaB"
                     /locus_tag="PKX09_00730"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOU16389.1"
                     /translation="MEARKFYIETYGCQMNEADSELVIGLLRGANYQPTAIPEEADLI
                     LINSCSVRENAEKRAIARLTQFKSLKKKKPELLLGLIGCVAQRAHDEILTENKFVDLV
                     LGPDSYRNLPEYLNQEQMPIVDVNLSRTEVYDELIPSRNSVVNAWVSIMRGCNKFCTF
                     CIVPYVRGRERSRSPQSIIDEIRQALNDGYREVTLLGQNVNSYHYGANRFPELLELVA
                     AIPSLRRIRFTSPHPGDVDEKMLAVMQSHSNICKQIHLPLQAGSSRILKLMNRSYDQV
                     HYLKVVNLIREFLPQVALTTDIIVGFPGETADDFEQTLQVMQIVKFDSAFMFKYSPRP
                     GTKAAKMTDDVPEEEKSRRLKAVIQLQKSHTLERNKALVGTVQEVLIDGTSKKNPDEM
                     IGRTESNKIVVIKEGHPKIGEFCNVQIEKAMGVSLFGKII"
     gene            complement(73726..74646)
                     /locus_tag="PKX09_00735"
     CDS             complement(73726..74646)
                     /locus_tag="PKX09_00735"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU16390.1"
                     /translation="MKKAPKLLFVVNTRKRVHSISLSWGKLVAVLAGGVFLLIFVIIL
                     GANLFSNLVYQSRLTRLKKNNNRLVSTFYDLNNRIQKMESDISMLVEKDKALRTYGDL
                     PVIDQDIRRLGVGGRLMPKSDELDQLLPESDIKVSELVNNLDRLAREIKFERISYETI
                     YDAFKNRSDQIQSTPSIRPVYTGFINDGFGYRRDPFTGRREFHYGLDITTPTGTPVFV
                     TADGVVGKSGYQGGYGYVVEVNHGYGYSTLYAHLSKILVRSGEAVKRGQKIGEVGTTG
                     RTTGPHLHYEVKQFNINKNPLDFFFSGYIR"
     gene            75049..>76714
                     /locus_tag="PKX09_00740"
     CDS             75049..>76714
                     /locus_tag="PKX09_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16391.1"
                     /translation="MKKGVYVLIMLVVLSTIVEVGWGQVSITQFETAYNQDFNSLANT
                     KTDNAWTDNSTISGWYSNRTEYIADNGSSTTGGLHSYGSADASERALGGLSSSSASPA
                     FGIRLVNNTGNTITSFNISYKGEQWRQTANSHTLVFSYQVDATGIESGTWTSFSDLDF
                     TALKTGTSSALDGNAEGNYQNVSSNVIIEVPNGHEIWFRWLKTGTNSPGLAIDDLSIT
                     AYEIKVPTISANPSSLSGFSYVVGSGPSAEQTFMVSGTNLTADISISAPEHFEISKTS
                     CSDFTSSLTFAQSDGSVNETIIYVRLKAGLAIGSYSSEKITCSCEGADDRTVTCSGTV
                     TSPLPNVWINEIDADQASTDSLEFIELCGISGLDITGYYIVLYNGSNSNKYLTHTVGS
                     FTFSNEINGYGFYVAGSNNIIQKDDTLGNGEENLIQNGDPDAVALFNSSKELIFWYSY
                     GGTFIGNAFDDFPPVSFYVSKSSIDIETTSMGFLKNTKPEDSETGNNNLLPTPGAANT
                     DGTTDQSLPVSLTSFSAQSRNQSVVLSWHTESEIENLGFIIQKRLPE"
BASE COUNT        22377 a        16821 c        14754 g        22662 t
ORIGIN      
        1 aaaccggatt ttatcaacaa ctttatatgg atttatgaat taagcaggtt agatacttta
       61 agaaaagatc aatataaaaa attagtccaa aaaataacta ataggtcaag ctgtctttaa
      121 gatattgcgt tttctaccta aaattagtgc gcgtttgctg acattgactt gttgaatttg
      181 cccagcaaag cttggtatac ttcagccagt ttggttttat caactaaaaa tatcaaaact
      241 gaagtcataa tgatactaag caatacaact gcataggtca ggttctgaat cacctctcca
      301 ccaaccacac cctgttgtaa cggtactgaa gctagtaccg ccgccgccaa tcctttcgga
      361 atgattacag aaaacaaggt ggcatcttta attggggtgc ctctcggaat tgttaaactg
      421 actacaggaa ttcgtatagc ataaatggca atcgttaata ataatccaaa caccaaaaga
      481 ccaatgccgg ataattgtat cgaaatacca atatagatga agaaaaaagt cttgagtaga
      541 aatgatattt cggagaagaa accacgctca cgactgtcga gttctgtggt ttccaaacga
      601 ccactgtagc ggaacattga aaatttaatt ttagatacat tgctaattgt aaaaccgaac
      661 accagtgccg caatggctcc gctaaaacca agcaattcga caattccgta aacaataaaa
      721 ataaaggcta acgtcgtaaa agttgagttt tccaaatttc taattcgatt aagaatcgct
      781 gaccaggcta acgctccgag cacgccaaat gcggcggcta tgataaaagt ggcaattatt
      841 ttcagtgtca ttaaaccgat tttgatctcg cctaatttgt aagcctggag caggctaaaa
      901 gtaaatacaa tacacaacac atcattgatg attgattcaa atattaggat tcgcttggaa
      961 tcctccagca tgtctaattg atcaataatc ggaatcacga ttgccgaaga agttccaccc
     1021 aaaattgcgc caaccatcag cgattgaatc aaaccgagtc ttgtaaatag gaaaaccagt
     1081 cctccaacaa cgataacaga aacaataaag ttcagaattg taagacttaa taaacctttg
     1141 agcgactgtc ggatacctct caggctaata ccaataccgc tttcaaacag aatcataatc
     1201 agtgtgattg tggtaaacac tcgtccgacc gctccgaaat gggctggttg cactaaaccg
     1261 agtatcggtc caatcaataa accaattaga aaaagaaaca gaacatccgg aattttcgtt
     1321 cggctataaa ttgctgaaaa taaatgagca caaaaaatta taactccgat taatattact
     1381 gttaatgtta cttccataat ttcactccat tctttcaatt aataacataa aacacttgat
     1441 ttatactgta aaactaatat tccaaatatc tcattatcga aataataaag gcgctattta
     1501 taggcgaaaa gcatccggta aatgttcaat ttgaacctcg ctaccgttta gattgacagt
     1561 tacgacatcg aaccgacaat caacattttc aactggattc tgggataaat agccagaggc
     1621 gatccgtcca atttggcgca tttttcgctc atctacccag ccttcaggcg gtccaaattt
     1681 tgtatgacgc tgtgttttaa cttccacaaa acagagatta ttatccttct gtgcgattat
     1741 atcaatttcg ccataagcgc caagatggaa attacgttga attatttgat agccgtgccg
     1801 aaatagataa agcgtcgcta aatcctcacc atatttcccg agccagcggg tattcgccga
     1861 cttgtttata tccacaatat attctgtaac cccgcgaaag gatatacgat gtattggaca
     1921 aggtttcaac ttcacgatag attcaagatg tttcttagtt ccgtaacctt tatgttcggc
     1981 aaaaccataa cccggaaaga cagaatcgta ctgttgcatc aggcgatcgc ggtaaacttt
     2041 ggcaataatc gaagccgccg caattgaata acacttacga tcgccgccga taatcgccgt
     2101 ctgcggatat attttatctg gcaaaggttt gccgtcaacc aaaatatgct gaacgttgat
     2161 ctttaagcgg ccaatcgcca tacgcatagc tttgtgcgtc gctctcagga tatttatacg
     2221 atcaatctct tccgattcaa ctaaaccgat gccaatcgcc tttgcttgac tcattatttc
     2281 atcgaacaat ctttctcgtt ttcgtggaga aatttgcttg gaatcttgga ttccactaat
     2341 aatttcactg cttttatcaa aaactactgc ggcggcaact accggtccag ctaatggtcc
     2401 gcgcccggct tcatccacac cggctatatg gcttactcct tgtttccaga gattattttc
     2461 aaaatccaga gttggaaatt catttttcat tttaataaaa atttaagatt cagtaccgga
     2521 ttaacaatga gagaaatttt tatctattaa atttattcat cggtaaaagg caaaacttga
     2581 ataaagtcaa attttgaagg ctttttacag atttagtcag aaaaatactt agattcaaac
     2641 tcgaaggaag ccccacagcg gtttaattca agtttttcta atcgtacaat taacgtacga
     2701 gttaatcaca agcaatgact gcagattaat atgctcgctt tgggccttta ataattgaat
     2761 aacatttata aaactttcct tgattattac tcttgctttt tttactttta tccaaatttt
     2821 tctaatatga ataataaaca ttttaaacac aggaggcaca attgactgag cataagttta
     2881 agccgttcgt accggctaat acggatatga aagaattcac tatccgctca ttgattatcg
     2941 gtttgattat ggcagtcgtt ctgggcgcgg cgaacgccta tctcggctta aaagccggaa
     3001 tgacgattgc cgctacttat cccgccgccg ttattggtat ggcgattttg cgtatgatga
     3061 aaggcacgat tctcgaggaa aattttgctc ggacggtcgg ctcgataggt gaatcggtcg
     3121 ccgctggtgc aattttcacg attccggcat tttatatcgc tggaatctgg acggattttg
     3181 ccactttacc gaattatctg attgccagct ccattatgct ggtcggcggt gttctcggtg
     3241 tactgttcgt tgcactcctt cggcgggtaa tggttgaaga tgtggaactg ccatttccgg
     3301 agtcggttgc cgcggctgaa attcacaaag ccggtcagaa aggtggtact ggagctaagt
     3361 ttctgtttgg cgcaatggga ttaggcgctg ggattcaggc tttgatttca ttaaaagtct
     3421 tcaccgatct caaggattgg ttcatcacct tcaaaaaagc cattattcaa cttggtagca
     3481 gtggttcggc aaccggccag agcgggatgg ttctaaccgc gccgcgcgtt agtccggctt
     3541 atatcggagt cggctacatt attggtccga aattagcttc gctcaatttc agtggcggtt
     3601 tgctggcgtg gggattattt gtaccgattt tgacttactt tattgcgcct tccatattgc
     3661 cggatacgac tgcagttacc actgacacct ggatggcact ttcatctcag gtctggcgta
     3721 atttagtgcg accaatagcc gtcggtggta tgttaatgag cgcaatctat actctcttta
     3781 aaatgcgtaa aagcctaatc aatggacttt ctcgtagtat cagcgatgtt aaaaaagccg
     3841 ctcagggtgt tcaagtcaac gaacgcaccg agcaggattt aaaattcacg actgttatgt
     3901 tcggtatttc tctggcagcg gtgggaactt tctttattta tttatttttt gcgaaaaatg
     3961 tcatagccgc attagtggca acggtcgtaa tgatcatcgc cggtttcttt tttgcggctg
     4021 tttccggtta tctggttggt attattggtt ccagtaataa tccaattagt ggactgacac
     4081 tttcaactct catcgtagcc gctcttttga tggttgctct cggtatgaaa ggcacgcccg
     4141 gagtagcggc cgtgctcggc gttgcaggtg tagtctgtgt ttctgctgca gtcgctggtg
     4201 aaatgttaca agacttaaaa gttggtcaca ttctcggcgg cacaccctgg aaaatgcaaa
     4261 tcggcgattt aattggtgta gctctttctg ccgtagttat gttcattccg ctcataattc
     4321 tccatcaggg cgacatcaat atgggcggga ctggtctggg cggcactaac cttgccgcac
     4381 cgcaagccag tttaatggca atgttatcac aaggtatcgt cagcggtgag atggcatggc
     4441 cattgataat cgtcggaatt ttgttgggac tgggattcat tttaatgcag gttaaaagcc
     4501 cgatgttggt ctgtgtcggt atgtatttgg gattggaaac aaccttcgcc attttcgtcg
     4561 gcggtttaat aagggggtta cttgaccatt atgttaccaa acgtcgctgt aatgatgccc
     4621 aaaaatcacg cgtggaaaat acgggagtct tgttagccgc tggattgatc gctggtgaag
     4681 ctttagttgg tttattagta gcattactcg ctattctgaa agtcggatac gacaaatggt
     4741 taccgagcat ttttaatttt cttccgttcc gattcgaagt cagtttatta attttcggtt
     4801 taattggctg gattttagtc agcattccat tgaaaaatgc aggcaaagcc gatgagccgg
     4861 cgccaccatc agccgttatg taatcacaag gaagttattt gatgaaaaat aaaaagggcg
     4921 aggtattgaa cttgtacgag atcgtgcctc gccctttagt taatagcgaa tgtcaggctg
     4981 atggcagaat cgtattactc aaacctaaat tcaaaaatcc atttttgatt aagttactcc
     5041 agcgcaatca acgcgataat ttttataaat tgaaactgga tgaaattggt tcggcttgct
     5101 ggataaaatt cgacggctat aaaagcgccg ggattatctg tgaagaactt gaaagtcagt
     5161 tcggtgcgca aatccaacca gttgcagaac gagttggaaa atttctcata atgctaaaaa
     5221 aagccaatct aatcgcatat tgatttctca aaatagttta caatttagcg agtattaaaa
     5281 aacattaaac ttgggatctc attcaatctc gattgaactg cctacgataa atttcctcgg
     5341 tcagtaatag cttagaaaac ttaaaatata gatatattac cgacttggga agtagtctgt
     5401 ctaattattt ataaaatatt ctcaaacaag aaataaccaa caaaattcgg attaatcttg
     5461 ctgacctaaa tcattcgact accgccaaat tccgacgaga aaatgttaaa acttttgatg
     5521 taataaccaa aaaatatatt acgtaataac gcccggtaat tttacattat tgccgttttt
     5581 tgggcattgg tacggtgtag acgtttgatg gcttttgccc gacgcattct tttttcgaca
     5641 ctcggttttt cgtagtatgt ccgtttcttg acatcattca aaataccagc tttttcgaac
     5701 tttttcttaa agcgtcttaa agctctttcc agtggttcat tgtcgtacac ggtaacttca
     5761 accatgcaat cagtatcctt tctactgttt agccgaggta agcttttaag ttcttacttc
     5821 gtgatttatg acttaaacgg cgaatagcat tttccttgat ctgacggact ctctcccgcg
     5881 tcagattgaa ttcttcgcca atttcattaa gagttaaagc atattcgcga tctattccga
     5941 aatacatctt tatcacttcg cgttcacgct ttttcagcgt gttcaaagcc gctcggattt
     6001 cctgtttcag cgactcatcc atcaacggat gatcgggtgg caattgcata tcgtcttcaa
     6061 taacgtcaat aagagcgttg gactcgccgt cccggaacgg atcatttagc gactgatgtt
     6121 tacgtgatat gcggatagcg tcggagactt cttcggattc cattgctaaa ccggaggcaa
     6181 tttcatcctg catcggtgct cgttcatact gctgttccag aatttcagcc tctttggtga
     6241 ttttgctgat tgtgccgacg cgattaagcg gcaggcgtac gatccgcgag tgttcagcta
     6301 aagcttgcag aattgactgt cgaatccacc atacggcgta agaaatgaac ttaaaaccgc
     6361 gggtttcatc gaaacgctcg gcggctttaa tcagccctaa attaccctca ttgattaaat
     6421 cggtcaaagg caaaccctgt ccctgataat ccttagccac actgacaacg aaccgtaaat
     6481 tcgattcagt caattcgcgg agtgcatccc gatcaccggc acgcaaacgt tgggcaagtt
     6541 caatttcctt atcaggcgac aaaggttcaa atttcccgat ttcctctaaa tatttgctca
     6601 atgcgcggtt ggaatcactg ccaacccttt tgtctttttt cgacataaac tacgaacctt
     6661 cgctttcact ataaattaaa agcctgcaaa aatactaaaa attttagatt ctaccaatat
     6721 ggagaagata atttttgatg cataaataat tattacttta atgcaaaatc tgccaaaacc
     6781 caaatattga gatcttcaaa gccctattat acaataatgt ttgtcagtac ttgccagtct
     6841 ctaatccgat caacaacctg ctgggcggta aaaccgaatt tttcggctaa cactttggcg
     6901 ggtgcggaag ctccaaagcg atctatggta atggtcagca atggattgcg gattaggtca
     6961 ccccaaccgg tgcttcgtcc agcttcgata actaccactg gcactttacc tttcgggatg
     7021 acggattcac gataggttgc gttctgccgc acgaattttt cgatagacgg catcgaaacg
     7081 acccgtgtcg cacgtccttc agcctgaaga attttctgcg cttcgatagc aaccgacacc
     7141 tccgaaccgc tggctaccaa gactaagtct aaagcattat tctgttctgg agaaattata
     7201 tagccgccgc gatcaatcaa atgacgatca aaattggcta ggcgattcaa cactggtact
     7261 ttttggcgag tcaaaatcaa ggcagtcggt ccccccagat tattgatagc agatatccag
     7321 gcttgagcaa cttccagccc atccgccggc cgaattaccg tcatattcgg aataatgcgc
     7381 aaagatgcta cctgctcgat cggctgatga gtcggtccat cttctcccac aaaaatactg
     7441 tcgtgggtaa aaacataaat aactcgcaac ttcatcattg ccgccaaccg aataggaggg
     7501 cgcatataat cggaaaaaac caaaaaagtc gctccaaaag gaattaaacc accgtacagc
     7561 gccaaaccat ttaaaatagc acccatcgca tgttcacgga taccaaagtg aaagttacgg
     7621 ccggaaaaat tatccggagc aatatcgcct aaatttttca aataggtact ggtcgaaggt
     7681 gctaggtcag ccgagccacc ataaaaattc ggcaaaagtt cggcaatctt ctgcataatt
     7741 ttaccggaga gagcccgcgt tgccagggca tcgggtggaa tggctttgat aatttcttct
     7801 tcttgttgaa tactaaggat ttgattctgc gattcgtcat atttttttgc cagttctgga
     7861 ttagcctcgc gccatttttg atattcgatc tgccactgag catatttttt ctcaagttca
     7921 gcgcgccgtt cagcgtaaac cgtgcgtact tcctccggaa cgtagaaaga gacattttca
     7981 tcaaagccga gattgcgttt ggtgattttg acttcttctt ctcccaatgg agcaccatga
     8041 cattcggaag tatcttgttt actgggactg ccacaaccaa tgtgggttcg agtaataatg
     8101 agttttgggc gatcaggctt ggataaagct tcatctaaag ccttagcgat actgatgtaa
     8161 tcgtgaccgt cagcttggag aacttcccag ccataggctt caaatctttt ggcaaccgat
     8221 tctgaaaaag tcaagtcggt tttgccttcg atagttatcc gattatcgtc gtaaaagtaa
     8281 atgatattgc cgagtttcag atgaccagcg aacgaagcgg cttcgtgcga aagcccctcc
     8341 atcaaatcgc cgtcacttac aattccatag attttgtgcg tcccaaaaat ttgaaattga
     8401 tcagtattaa agcggctagc ggtaattttg gcggcgattg ccatcccgac accggtggca
     8461 aaaccctgac caagtggacc ggtagtcgtt tcgacgcccg gtaaacattt atactcggga
     8521 tgacccggcg tgcgcgaatc ccattgtcga aattgctgca aatcttccag agtgacatca
     8581 tatccactca gatgtaacaa tgagtacaat agcatcgaac cgtgtccggc tgaaaggata
     8641 aaccgatcgc ggttagccca ttgcgaatca ccgggattgt gttcaagata cttgctccac
     8701 aaaatagtag cgacatctgc cattcccatc ggcattccgg gatgccccga gttagctttt
     8761 tggacaccat caaccgccaa tagacgcaat gtattggcac acagacgagc catttccaga
     8821 ttaatgtttt gcttactcac actttgtcct ttcctttttt agttcagtaa ttgataaacc
     8881 tactttagtt ccaatatcat ttaaagtctg aaaaacttta tcttgtaatg gtacaaaatc
     8941 gcgatacctc tcttcggcga gaaattcctt ttcaccggca acataaatcc gttcctggcc
     9001 ggcggctttg tcggcatttc ttaacatttg gatatattta tcaattcctt cgctaattaa
     9061 atcagttccc acaaatgcgt ccggatttat cgcgccgata aagtgagata ccccagccgg
     9121 tgcattggaa tagcgggcaa tttcggttcc aaccattccc gcagacaaaa ctccacacaa
     9181 aatatccact accgcgctca gaccataacc tttatgtccg ccagtcagtt cagtaccgcc
     9241 acccagcggc aataaaccac caacccgacg gttaatcata tcatcgagta aagccgccgc
     9301 gtcggtagaa ggtttgccgt cctcatcaca agcccaacaa tccggaatcg gttcaccttg
     9361 gcgcgcataa acttccaatt tgccgcgcgg cacggttgaa gtcgccatat ccagcaagaa
     9421 cgggcgctcc tctttaccgg ggaatccgat tgcaatcgga ttagtgccga tcgtaacgtt
     9481 tcgtccaaaa gtcggcacaa ccaacggcgt ggtatttgtt aacgaaactc ccatcattcc
     9541 ttccgccagt gccattaaaa cgtaataacc ggcaatccca taatgattcg aattccgaat
     9601 tgccgcaaaa cacatatagg cagtgcgcgc tttctcgata catttctgca tcgcgcgata
     9661 agcaattggt tgccccatac cgccatcgcc atcaataacc agtgaaaccg gcgtctcgcg
     9721 aattattttg acatccggat taggtcgaat gaccccgcta tctaaaccgt caacatatct
     9781 tttcaaacgc gccacaccgt gtgagccgat tccgcggcga tcggcgcgaa ttaatacctc
     9841 cgccgtaata cgggcatctt cggcattcaa gccatgggca attaaaacct gccggacaaa
     9901 attatccagt tcggttacat ttacaatgac ttcttttgaa ataacgcctc ctatatcaaa
     9961 ttaaattcgt ttgcgaaaac cctcaccaat gacttcatgc acatttgcga taataacgaa
    10021 agctttcggg tcaatcttaa gaatttcctg tcgcaaagtt gcaacctccc gacgagtcac
    10081 caccgtataa agaacattgc gagttttacg ggtataaaac cccttcccga gaagttcggt
    10141 tccaccccga tccatctctt tagtaatcaa attgatgatt tcatcctgtt tttcactaat
    10201 aatataaaaa gagcgggcgt aatccatacc atccaggatt aagtccagca ctttactgga
    10261 aaaatataaa ctgatgaaac catacaaagc caggttgacg tctcggaaag ttatcccggc
    10321 aaaagtaata acgaaaaaat ccgcgatcaa tatcccagtt ccgaccgaaa cattactgta
    10381 tttatggata atttgcccga cgatgtccga gccaccggtt gtccctttaa agcgaaaaac
    10441 gactcccagt ccaactccca agagaatacc gccaaaaatt gaagccaata acggatcctc
    10501 agttgccgcc tgtactccca aaactttatc ataaaaatcg gtaaaaatcg aaaccaacac
    10561 aaatgagaaa aaagttcgtg gcccaaactc cttgcccagt attttcaatc ctaaaaaaaa
    10621 cagagggata ttgaaaacca acatcaccag accaaccggt aaattgaaca agtaatgtaa
    10681 aacaattgat aaaccgctta ctccaccggg agcaattttg gctggaatca agaaaaaaac
    10741 cagactgcca gccataacag ccgaaccaac cgccaaccca caatattcga aaatgatgtt
    10801 ttttattttt tgtttatgga ccatataaaa ctatattata ttttcaattt tactacttat
    10861 tacggaaaaa aatacgacca tagtttaata aatttttaac aaacttttat aataagctag
    10921 ctaaattatt tacagttatt gcctattcca ttatttcaga ataatttcat agggttacaa
    10981 ttggagaaaa acagtgcaga ataaccagaa tcctattgat aataaagaaa ataaaagaac
    11041 tgtcagaatt tttgcctggg cttcattcct gaatgacatg ggctcggata taatttatcc
    11101 ggtttggcca ttattcgtta ctgagattct gaaggctaat atggcggtgt tgggatttgt
    11161 tgatggattg ggagaagcgg ttgtttcttt agccaaagca ttgtccggct attggtcaga
    11221 tcgtatccgt aaacgaaaag tttttatctg gagtggttat ttgatgggag ccgcatcgag
    11281 aatcggctat gccatttcca cagtctggca acatttgatc ccgtttcgaa ttctggaccg
    11341 cgctggaaaa atcagaggtg cgccgcgcga tgctattatc gccgatgtct caagcaatga
    11401 caaccgcggt agaaatttcg gattgatgcg gatggctgat aatttaggtg ccgtggtcgg
    11461 tattttgatt tgtttagcat tattcaaaaa aatcggatat cgctcccttt tcatactggc
    11521 ggcaattccg tcgcttattt ccgtgttgtt aattttaatt aaaatcaaag aacccggtca
    11581 tactgaaaaa gtcatttata aaggaatgac tttacgcgat ttagaccgta atttcgtttt
    11641 atttatgatc ttaagcggaa tttttgccat cggtaatttt acctattcat ttttactaat
    11701 acgcgccaaa cagatcggtt tccaagaagc gtttgtgccg gtgttatact tggtttatac
    11761 cctttcggca tcactaacag cttatccttt cggtcggttg gcggaccgga ttggtcgtaa
    11821 aaccgttatg caaatgtcat ttctgctctg gggattggtc tgtcttgacg ttattttctt
    11881 ccaatccaag attatgatat ttttaatgtt tgttatttac ggtttgcata agggcgcact
    11941 cgagccggtt cagagtacaa ttgtttctga attagctccg gaaaagttcc gcgccagcag
    12001 tctaggcact tatcaaatgt taatcggatt atgcgctttt ccggcgtctc tgattgctgg
    12061 attcttgtgg gataaaatcg gcgccctcgc accattttat ctatcgcttg gattgacgat
    12121 tgtagctagc gctttgatga tctttgtcaa agagtcaaaa attattacta aatccaattg
    12181 attatttttt caaacgattt aaatattcgg aataattggg taaggtcggc tggtaaaccg
    12241 aattcattag cggcgatgaa ataatgtaat cagctgtcgc ccgattacag gctaccggaa
    12301 tattccagac aaccgaaatt cgtagaagcg ccttaatatc tgggtcatgg ggttgagctt
    12361 ccagcggatc ccaaaaaaag atcagtacat ctattttgcg ttgagcgatg gccgcgccca
    12421 attccagatc acctccgagc gggccgctct ggagtttatg gattttgaca ttcaattcct
    12481 tttcgagtag ttttccagtg gttccagtcg catgcaattt atgttgcttc agcgtacctt
    12541 cgttgaattt tacccattct aacaagtctt tctttttatg gtcatgcgca accagcgcta
    12601 tattctttgg cttaggtttt acttcattgg tatcattcac ttccattgaa tttctcctta
    12661 tactttatct tttttaatta taaactaatt ccctctgaca tccctgcccc aattcagttt
    12721 ggtgcgcaaa gtatgaaaaa aggaatcgcc gctaaagcgc acaattcgaa gtggaaattc
    12781 agctttatga atctcgattt tttgagccgc gccgagggca attctattat ggccatcact
    12841 aattagcgaa acgtcgtcac ttaaatcggt acaatcaatt cgcaccacct gatcggccga
    12901 gataaccacc ggccggactg atagactatg cggacaaatc ggacaaatta ccagcacatt
    12961 taattccggt gaaataatcg gtccaccggc agacagagaa tatgccgtgg aaccggtggg
    13021 agtcgcgatt atcagtccat cagcgcgata ggtgttcaaa tattcttcgt tgatataggt
    13081 gcgaatctta atcatcgtgg agatccgccc tttgtcaatc acaaagtcgt taaaggcaaa
    13141 actctcctgt atggaatctt tgaagtaagt gcgagccgtc aatccttgcc gctcttcaac
    13201 cgaatattta ccattaagaa aatctatcaa acgctcgtga taattatcaa cagatacatc
    13261 agctaaaaat cccaatcctc ccagatgaat tccaagcact ggtttacgac tttttgccat
    13321 taaatgcgaa gcacttaaga aagtaccgtc gcctccaatg ctgaaaagtg catcaatttt
    13381 ctcaggcagt tgctccggat tacatctttc aacaccattc aaattggtaa caagactatt
    13441 actttgccaa tcggcgacga ttacggtttg cttattttca atcaataatt taataatagc
    13501 cggaactatt gggtcgttta gttctatttt cggtgataaa agtaaaccga ttttcattct
    13561 atatcatccc taatcgaata agttgttagt ttctggagtt tcgcccgatt ccggaaaatc
    13621 ctgttcgcag atttcgccgt cgctcttttc gatcaacagt ttcactcgct tttccagtgc
    13681 ctgtaaatgc gcctggcatt cgctcgatag gcgcattcct tcttcgaaca gtttgattga
    13741 ttcttcgata tctttttcac ctgattctaa aatctgtaaa atttcctcaa gccgctcgag
    13801 acttttttcc aatgaaaaac tttttttcgc catttattta cctcgttaat taactaattt
    13861 ttatgtgatt gaattatctc cgttttagcc tggaattcac catctttcag ttccacgctt
    13921 acttgttcgc cgactgctac ttcactgatt gatttaatga tttttttatc cggccaatga
    13981 tacacaatgg caaaaccacg ttccattatg gctcgtggat tcaagactac caatgttttt
    14041 tgaatgttat ccaattcaac ctgggctgat aggatccgag agttaatcgc tttgataatt
    14101 cgctcttcta attcgtcaat ccgttgaata cgttgacgta acaattccag aggacggttc
    14161 aagaaaagac gtctggcaat attatcaatc tccttttgaa aattggatag ccgcattctg
    14221 atattttgag ccattttatt acaggtctga tctataaagt ttaggatatg acttctttca
    14281 ggaatcgtca tttcagccgc ggctgaagga gtcggcgccc gcaaatccgc cgcaaaatcg
    14341 cagagggtga aatcaacttc atgtccgacc gcactgacga tcggaatagc cgattgagcc
    14401 acagcgcgga ccaacggttc ttcattgaaa gcccagaggt cttccaatga accgccaccg
    14461 cgggtaatca cgataaaatc tacatttcgt aaacgattga aaagttttat accggcaatt
    14521 atagttgcgg ctgcttccgc tccctgcacc aaagccggat agatatagat tgcgatcgag
    14581 ggattgcgtc gcctgcttat ctgcagaaaa tcacgcaaag ccgcaccgct ttcactggta
    14641 attaaaccga ttcgggtggg ataaagtgga ataggctttt tgcgttcccc atcaaacaag
    14701 ccttccgcgc tcaacttttt cttcaaagcc tcgaaagcca gaaataaact gcccaaaccg
    14761 gccgcgcgca cctgctgaac attcaactga tactgcgatt gcatcgcgta ggtcgtaata
    14821 tttccaaaaa caagcaggcg cattccgttg acaatttgaa aaggaataga caatgattga
    14881 ctacgccaga tggtgcagtt cagcactgcg gtttgatctt ttaaagaaaa atagatgtga
    14941 ccggctcgac tgatggtgaa attcgatact tcaccttcaa cccagaccgg cgcgataaat
    15001 tgctctacaa cccgcttgat atctcgggca atttcagtga cggtataaat tcgctctgcc
    15061 ataaaaaaaa gagtggtgcg gacacgaagg ccatcaccac tctccttttg tacaaattaa
    15121 ctacctaatc ttcttcttat gacggtctct tctaagccgc ttcttacgct tatgggtagc
    15181 aatttttctt ttttttcttt ttcttccaca gggcataacc catttactcc tattatttca
    15241 ctacaaactc taatgctggt tatagttgcg actgatctca cctttaaaca gtttagacgg
    15301 tttaaaaaca ggaactatac gcgcgggcaa actgatttct tcattcgtcc ctggattacg
    15361 ggctttttta ggtttgcgaa cttttaaact aaaattgcca aaacctctga aatccacgcg
    15421 ctcaccgttt agcaaagcat ccttgatggt ctcgataaaa ccattaatca ctacttccgt
    15481 atcaacttta gtcaatccgg tagctcgtgc tacaatatca actacatcgg cttttgtcat
    15541 attttctcct ccaaatttcc ggtacaaaaa ccggggatta ttctaatttt cttcaaatta
    15601 tgcagtcata aaagcattaa attgattaat gagtctatga tcactttgaa aagtccgccc
    15661 cgaaaaaatt ccctattgct gttcctgttt accaagttat caaatattct caatgttaaa
    15721 aaaacttata ttttagattg taaaaatacg tcaggcattg atgattaaca agcctttttt
    15781 caaattcggg caaaaggatt tgccgacttg gactagattt acggcgcgta aatcttccgc
    15841 atatgttccg atttctgaat tagacgttcg atttctataa cttgcttttc tgaaatctca
    15901 agtgcaccgg caatttctgt aacctcccaa ctctttgcga aagcgatcaa aattaaatcc
    15961 aacttctcat aggagatgga gagcgctttt tcgtcggtaa gtcccggaag aatatcgggc
    16021 gaaggggctt tttccctaat ctttgccgga atattcaggt atcgagctaa ttctcttacc
    16081 tgagtcttat acaagttcaa cagcggcaaa atatcattcg ccgaatcaca gccgtattta
    16141 acaaaaaaac cggtctggta ttcgctttta ttagtcgagc caactaccaa cctattttca
    16201 agctccgcat acaaataaag taaaagcatt cgcagtctgt gtttaacccg ataataggca
    16261 ttgctttttt taagtatgaa tcaaattcct tatctcttaa ccccgtgaga ctggcggaga
    16321 agggagtttc cccggttttt ctccgataat aatcgtacgc ttttccaatt aaagttccct
    16381 ttattttttc cggtaagaac ggcttgttta caggcgataa tttatatact tcaagcatct
    16441 caagatacgg cgttaggtca attaatttcg ttgcaatgcc taattcattg gcaaaaattt
    16501 gcgcatctac ttgatgctcc cttctggaat ctttttccgg taggattaaa gccaacgtat
    16561 tttccgcacc gacggctctt ttacacaaag cggcaatcac ggctgaatca attccaccgc
    16621 tcaatccaat tattactcct tccctttcca gctttttcac atacaccttg aaaaattctg
    16681 ctaaagaact ggctaattcc tccggttcga ttgccattga attttgtaaa tattttaatt
    16741 tcgattccac gttttctctc aaatattcag caggctcagc cttacttatc aaaacttaac
    16801 acagtttagc aatacttttg ataaatttgc tgggattttc tcttttgctt ctaaagaggg
    16861 aaaatactgc ggcaacgcca gctcggactc ctccatctag taaagcaggt aagtaatttt
    16921 ttaatctttt ccagcactaa taaacattaa acaatagaaa aatttattaa caatccaaat
    16981 agaataaatt ccagttaaac cggttaaata accggaaatg aaatttccga tccaaacgtc
    17041 agtaacttct tttaattaaa gatgataata ttgaaataaa tttagaagtt tttagcggac
    17101 gaggcgtccg attctccacc agcgaatttt cttatccagt tgatagagcg gacgttcttt
    17161 atcccaggac atatacgtaa tcaatccccg tcccgtaacg ttatcgaatg gcacaaagcc
    17221 ccagaaccgg ctgtcccagc tattatcgcg gttatcgccc atcataaagt agtgatcctg
    17281 ttcagcaatg taatactcgg ctggattacc gtcaatatac attttcccct gataaatgcg
    17341 gaaggtatga ccttttagct cagccgcatt tttgatgata ccggtttgaa tttggccaaa
    17401 acgaagcgtg tcgcctttag ctggaacata aatcggtcca taattatctt tattgccgat
    17461 gccgtcggga aatatctcag gatcattcca accactgggc gctacatact gcgaaataaa
    17521 cttcgcatgt ttaggattcg ggaaaatttt accatccaca tataccactt tgtcatggat
    17581 ttcgaccgat tggccaggcc cggcgataca gcgcttgaca taatttagcg acggatcaac
    17641 tggatatttg aaaataacga cctcgccctg ttccggcttt ttaaatgacg gcaaacgaaa
    17701 ccaagggata tgaaaaccag tttttgtcca gggaattccg atccaatccg gtgtccgagt
    17761 gccataaatg aattttatac cgagaataaa atcaccggtc aggatggtat tttccatcga
    17821 accggtcggt atttgatagg cttcgatgat ggtagctttt aaacctaaag ctattagcag
    17881 gacaattccc caggacttga cctcctgcca gaatttgctc ccacttgtct tcttttcttg
    17941 cttttgttct ttcatatttt aatagcgtga atttacaatc tttaactcgg agaaccaatt
    18001 aaagttctta aaattattca aaatttctcc tcagattaga aatagcttac taatcgaatt
    18061 catattaatc tgagtgatgt aaatattgga tttatcgctt aaaagttcta aaaattgaat
    18121 agatcgaaaa taaagaaatt cccccgcaat taacttttta tagttcaaca ataattccta
    18181 tcattttctc cgatttgacc ttaaatcatt ttattcctct caaatttgat agcggattag
    18241 caaataataa aatcctctct aatatctttt aatttattaa attagatacg ctttccaatt
    18301 gaataataac ttccggatta ttcaatttac ttggaattat ttggagaatt tgattaaaat
    18361 tgcgcgcttt ttattttaat tggcttttat cgtgatggtt aaaaacgtcg tcgacatact
    18421 aatccaaaat cgaaacaatc gggcggactg gccggctgta atttttaatg aacaaacttt
    18481 cacctattct caaatttacg accgatctat ttacctagcc gagtttttac aaaaacagaa
    18541 gatacaagca gggcaaacaa tcgggttgtt atttcaaaac aagccggagt atctaatcgc
    18601 ttattatgct tgccttttga acggtttttt accagtactt ttgccgactt acctctccac
    18661 aaacaaactc acctataact ttaataagct aaaaattgaa gcggttcttt atgactcgga
    18721 atttcataag gttgtggatg aaatcgaact gaaaaaaggt tcgcctctca taaaagtacc
    18781 atccagcacc tggcaagata cttcgattga taagactaat ataccgacgc tatcaaccga
    18841 ttcgtttcca ggaattaatt acacaaatga accagctgtg gtatcgttca ccgacggcaa
    18901 ttccggtttc cctaaagccg ttgtacatag tcaagccggt ttacttgcca atgcccaaaa
    18961 ttgcgtgaaa ttgttcggtg gtatgcgcac tgtgcgttta ctttgtaccg taccggtttt
    19021 tcattttatt tcccacagtt ttatcccgca ttctattagt ttagcgggcg gaactgtaat
    19081 tttagttgaa aactcaaaaa cggaagaaat cattcgggca ataaattcaa ataaagtcaa
    19141 cactttgatt ggaactccgg cttttttcaa ggaattcatt aactatgcca aaactgacga
    19201 acttagttcc ttgaaacatt gtatcgtttc tgggagctgt ttaagtcagg aaattcgccg
    19261 gaatatgata aaaaatctcg gcgttttcgt ctcggaactt tatggaacaa ccgaaaccca
    19321 gatgatctcc gtcgtctatg atgaaattga tcaatgtgaa ggtagcatcg gtcagccgtt
    19381 tccggagatt gacattcgta tcatcaattc atccggtgcg attgttactc cgggttcagt
    19441 cggtgaattg caagttcgct catcagcctt gatgttgaat tattttgatg acttggataa
    19501 aacccatttt aatcaaacca cctggtttag taccggggat attgtgagaa aaaccagcga
    19561 cggctcgttg atttttgaag gaagaatccc tgattccatc cataaatttg gttatgacat
    19621 caatcctgca ttgatcgaaa aagttttacg acagcatccg gcaattcacg aagcagtggc
    19681 ttgccctatt aaaaacccta atcacgaaga tcagattaaa gtgtgggtat ctttgcggga
    19741 atcagcaacc gttagcattg atgacttgca tgatttctgt agaaaatatt taccgggtta
    19801 tcttcaacca gatgcgatcg aagtcgtaac atattttgaa cgagacctat ccggaaaaat
    19861 tcttcgtaat ctgcataata aaagttaaat tttcacgata atatggaggc taattttgaa
    19921 agaatatcaa actgaagaaa taagaaatat cgtgcttttg ggacatggtt cgtctggcaa
    19981 aaccagtctg agtgaggcaa tgctttactc tgccggcgcc acgaccagat taggtcgggt
    20041 tgatgaagga acaacagttt ccgattatca tgatgatgaa atcgagcgta agttttcgat
    20101 ttcctcttca ttgctgaatt gcgagtggaa taaaataaaa ttcaacatct tagattcgcc
    20161 tggttactta gatttcgttg gtgaagccaa agctctgacg cgagtggctg acctagcact
    20221 tatcaatatc aatggtcagt cgggagttga agttggcact gaattggttt ttaaatttgc
    20281 caaagaggat gatttaccgg tttggtttat tatcaatatg ctcgacaaag aacacgcgga
    20341 tttccagaaa tcgctgaatt cgattcgttc ttcctttacc aaaaaagccg tgcaaatgca
    20401 gattccggtc aatagtggtc cgaatttcga tactattgcc gacgttataa cccaaaaagt
    20461 catcaaatat aaaaatgacc aaaaaggtac ttacaccgag ttagaaccca ccgatgaaat
    20521 caaggaaaga ttaatcgctc tgaaaaatga attaatcgaa tcagtggctg aatctgatga
    20581 tgttttactt gagaaatatt taagcgaagg tactatatcc gaagaggaat ttttaaacgg
    20641 cttacgcaat gcgattatca agcgcaatct gtttccggtt tttgtagccg ccggcctcaa
    20701 caatatcggc gtcacgaaaa ttatggatca tatcgtcaat tttgctccaa caccttccgc
    20761 taaaatttca gtcagcggca gttataatgg cactccgata acccgtaaaa ttagcgatag
    20821 tgaaccgact tctcttttca tttttaaaac tatcagcgaa atgcacgttg gagaattatc
    20881 catatttaag gttatttcag gttcgctaaa aagtggagtt gatctgctca attccaacaa
    20941 tggcaaaatc gagcgcatcg gtcaaattta ttgtgtcaac ggtaaagaaa agcaagaggt
    21001 tgctcattta catgccggcg atatcggagc cgtcgttaaa cttagatttt cgaaaacctc
    21061 cgataccttg tgcgatcagc gcgcaccgat cacttacccg ggaatttcat ttcccgaacc
    21121 agtcattcaa atcgctatag agccgaaatc gcggggcgac gaagaaaaaa tcagcgccgg
    21181 tttacagtcc ttaacgagtg aggatccaac aatgtcctgg cattacgatc ccgagcttaa
    21241 gcagacgatt ttgtctggtc aaggcgaatt acatttagcc attattttac aacgtttaaa
    21301 acaaaaattc ggagttgatg taaatcaact gcctccccgc atcccctatc gagaaactat
    21361 aaaaaaacgg ggtgactcca aatatcgcca taaaaaacag actggcggtg ccggacaatt
    21421 tgccgaggtt tggatgtacg tcgagccggg tccgcccgga agcggcgtag aattcaccaa
    21481 cacgcttgtc gggcagaatg ttgatagggt gtttgtgcct tcggtcgaaa aaggcgttcg
    21541 ctcagcctgt gaagaaggaa ttttagccgg atatcgtgtc accgatgtca aagccgtatt
    21601 ttacgatgga aaaatgcatc ctgtcgattc taaggatatt gctttccaga tcgctggcaa
    21661 aggcgctttc aaagaatgct ttatgaatgg caacccggtt ctgctggaac cgatttataa
    21721 tctcgaggtc attgtcccag aagaatatat gggtgacgta atgggtgata tttcagtaag
    21781 gcgtggaaaa atccagggag ttgattctga aggtccattt caaaaaatta ccgccaaagt
    21841 acctctggct gaactctatc gctatagcac ggctctacgc tcgatgacac aaggacgcgg
    21901 tatgcatcgt cagagttttt cccattacga acaaatgccc aaagaaattc aggataaagt
    21961 cgtagcggct tccagaaaag aacacgagga gtaaatccag tggaatttga taaactctgg
    22021 gcgccctggc gaattggtta tattaccaat cccaaatgtg aaggatgctt tctttgccgg
    22081 tatccatccg aatctgatga taaaaaaaat ctaatccttc agcgtggcaa atcggcattc
    22141 gtaataatga atttttatcc ctataataat ggtcatctaa tggtcgctcc ctatcagcat
    22201 acgaatgatt ttgccgcttt agatgatgct actcacctcg aattatttca tttagtcgaa
    22261 aagtcggttg gtattcttcg tgaaactctc aaagcggaag gattcaatat cggtgtaaat
    22321 ttgggaacgg tcgccggcgc tggtctaaaa gatcatctgc atatacatat tgttccgcgc
    22381 tggaccggcg atactaactt catgccggtt atcggacata ccaaagttat aagtgaagga
    22441 ttaactgaaa cctggcaaaa acttaagtct aaattcgatg aatgattttt gatagttgaa
    22501 tatcgctgtt aaatgattta aatttgtatt gcttttttag attatgaatc tggagtagtc
    22561 ccgcccaggc gggatggggg ttcgagtccc tcctctttaa ggactttact gccacttcaa
    22621 ttctgttttt tcttaaatga attttctgga gtagtcccgc aacggcggga cgggtggctg
    22681 ttannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcaaggg cgggatgggg
    22801 gttcgagtcc ctcctcttta aggactttac ggacacctca attatgctct ttcttaaatg
    22861 aattttctgg agtagtcccg caacggcggg acgggtggct gttatcccgc cggagcggga
    22921 caaaagtccc gcaagggcgg gatgggggtt cgagtccctc ctctttaagg actttacgga
    22981 cacctaaatt atgctctttc ttaaatgaat tttctggagt agctcaatgg tggagcgggt
    23041 ggctgttaac cactaggctg ggggttcgag tccctcctcc agagcaaaat atcaagtgga
    23101 aaaatatttt ctctatattc tcttctcgaa taatctaaac cattattacg ttgggatttc
    23161 atctaatccg gaaaaacgtc tgcaatatca taactctcag aacaaaggct ggacaaaacg
    23221 tggacgtccc tggcaattaa tcttccaaaa ggaatttgaa tcaaaatctg tagcaatgtt
    23281 ctgggaaagt tggattaaga agcagaaaaa acgaacaata attgaaaaaa ttatcaataa
    23341 ctctttcaac tggtaattat cgcattaggg agcgggtggc tgttgtcccg ccggagcggg
    23401 acaggcaatt ttggagcgta gtgatcccgc agcgaagcgt ggcggggtca ctgcttgcat
    23461 caacgtagtt gatgcactcc atatattcta tagttttttc ttctttggag cgtagtgact
    23521 ccgcagcgaa gcgtggcggg ggtcggtgca ctccatatat tcttatgaaa gaatgtcttg
    23581 tcctaaaata tgcggtattt aattatctaa ttgacagaag caatataaac ttcagcccaa
    23641 ttttgcgatc gaataacgta ttccttaata caagccggta agagaacact ctcccctttc
    23701 ttcaatatta acttttctgc cgaattccaa attaattcta ttctaccgtt tagaattaat
    23761 ccaatttgaa ctttatcgtt ggtataaatt tttttcgcaa aattttctgc gactttcata
    23821 ttcgctacta caaattcttc agcagaggat ctatatatac taattccatc ccgccaatct
    23881 ggatttacaa cttgaacctc attaggtgaa aaatcgagaa tttccagcag agcggcaaca
    23941 tctttatatt tatttgtcaa gccaatccgg acgacgttat ctgaattagt catacactca
    24001 attatatttc cagaaatata ggcatgcgga atacctgccg aaatattaag cgcctgtccc
    24061 gaggtcaatt ggaccaaatt cattaataat attaaaatca aaccgatatc ggaagatgga
    24121 tagcattttt ttaattctaa aaacagctgt tcctgttccg aaattcgatt accacgagta
    24181 agaatgcttt ctgcaaggga ttcgactaaa tttccgtata attgcggttc gctaacagat
    24241 ttccggatta aggttgaata aaactgtttg acgatggtgg tttcatccgc ttctgaaatt
    24301 gtactatcgc ctggcaatac cctatctgta aaagattgga ttggaggata caattcgagt
    24361 tttttcctta attcaacaat atttagcaac cctactaaag ctttaagatt atctatagct
    24421 attgctattt ccaatttgtg gttggaatcg ggataatgaa ttgagtcacg ccgatgtaat
    24481 cgaacagctt gtgatttatt gggatgcgct tgaatagaaa gtggtttgcc tgctgatagg
    24541 actttgaata aaaatggcaa attggcgcca aatcgtttaa aaacggtctc accgagaatt
    24601 tcaacaggat gttttttgat caattcagtc aaatcaataa ggtcattgga aatatttacc
    24661 tgtgaaggcg ccgacggatg ggcgccgatc cagagctcag catagggacg atttccttca
    24721 gactgtattc ccaataaacg tgggatgaat gcctgctcgc cacgcgttcc ccattcatag
    24781 tgttgaattt tatttctcag aacgaacggt ttaatctcaa aattattttc catcatgttc
    24841 taaaataatg ttttagtaac aattttacca atggattttt caataaaaaa ggtctgcgga
    24901 gagcagacct gcgagtgagg aggaggaagt tatttttcaa ttatgattgt ttagatttag
    24961 aaataatcaa taaaatcacg ctattttacg actcagacaa aaaatagttc tcctattaga
    25021 aaaataattt taaattgaac cgtgcacttg gaaaaagaaa cttcgttgaa taatgaagca
    25081 atcaaaattt cggtgatagt cgtcattgcc gcttatatat cggctcaaat gctgtcggat
    25141 attacaagtt tgaaaataat ttctttacta ggcttttcaa tcgatgccgg tacactgata
    25201 tatccattta cttttaccat gcgtgatctt gtgcataaaa ctatcggatt gaaaggcaca
    25261 cgtatagtta ttatatgcgc ggcggtgatt aatctattaa tggcactatt gttttggata
    25321 gtctcttata tacccggcga tccgaatgta ggtccacaat tagaatttgc taaggtactt
    25381 tcgccggtgt ggcgaattgt tattgccagc ataatcgccg aagtcattgc cgaattatta
    25441 gataccgaga tttatcgcat ttgggttaat aaagtcacca aacgtcatca gtgggcacga
    25501 gttttggtaa gtaattcatt gagcattccg attgatagcc taatctttgt atggggcgcg
    25561 tttggtggga tttataatcc gtctgtcgtt tggtcaattt tcttcgccaa tctgattttg
    25621 aaaggcgcta taactatcat tagcctaccg atgatctatc tggtgaaaga caagaactac
    25681 tgatatagta aagtaatgac ttcttaggtt gaataatatc agttattttt ctgtaaaaca
    25741 catcttctag tcaggctata tttcccagga ttccattggt aataaaagat tccggatggg
    25801 aaattttcag ccgtccgctt agcggtataa tacctagctg gttatttcga aaatatcatc
    25861 aacgaaaaaa accagtcaaa cttgcatcgt caataatacc atagctaaaa atccaactga
    25921 tgactacgct atcctcttaa tgatttggca aatcaaattc aacatccagc tagccactac
    25981 tacctccacc ccagccagaa atacttgaac caagattcca gccttcttcc ggattcgaaa
    26041 aatcgaataa atatatccag aaaaatgggt aatgtgagat tattcgtatt ttattacaat
    26101 cattcttaga acattagatt tatttctaaa tcaactaact attgcaggaa gcaaaatact
    26161 taataatatc ctttctcttc aagattattg aagagatatt tgtgataaat aatgacttag
    26221 gctaattcta ctcttttttc ccgcagactg gctggcacat tatgctgaca attgcataaa
    26281 gtgcgcaaac ggcggcaaac atcaagaagc gagacggttt gaggtcaaat atcataaatc
    26341 caaacaattt ctcgaggatt ccgagtagca tactaagaat tccaccataa taaaacacac
    26401 aacacgccca cttttgacac ataataaatc ctcctttaca tttattgttt catttttcaa
    26461 aacctaacta gttaaaatat aatttgtaaa atattcattc acaagaatct tattgccggt
    26521 cgccaactca aatggacgcc tcgatttggt cgaagtaact ttgcccgaat tatgggtctt
    26581 tagccgttga gatacatctt ccgacatacc aacgtaataa cgaccatctt ttagactacg
    26641 tattacgtaa accgaccaca tttgtagcgc ataggggatt tgaacccctg ttaccgacgt
    26701 gagaggccag cgtcctaggc cactagacga atgcgccatt tcaaaaaacc gcccgaggcg
    26761 gctttcgctg ggaagcaggg attcgaaccc cgatactcag atccagagtc tgatgtcctg
    26821 ccgttggacg acttcccaat ttttacaaaa agcggactaa atttacaccc caaacgacga
    26881 aatgagcaaa taaatcaacc ggtgagtcca gccaatataa aggaaatcgc cagcgtaata
    26941 gtcatcagaa attggagctg aatcgtatta gcactgagag gaaccatctc cttaacgtca
    27001 ttgaacggct ttttcgagag aaaaatagtt ccaatgtatt taggcataag gcagagaata
    27061 atcaacgacc agtatggaaa atatccgaca attacaaaaa ctataatcca aatcggcacc
    27121 acatataaaa tcaaacgata tattttaaga gccgtattac gattcgcgat ttttacgata
    27181 attgtattct tatcagccga gcggtcggct ttctcatcca gaatttcatt agcatataga
    27241 atcaacatca ccaataatcc agccggtacc gaagctaaga acgctaaaaa ggaaattgtg
    27301 ccagtctgga cataggatgc tcctattact atcaatggcc caaacgcaat aaaaatcccg
    27361 atttctccga gaccgcgata tgccaacttg acgggaatcg cagtgtacaa aaaaccgatt
    27421 gccaatccga ctattccaaa aataattagt gctggccgac cggtatagac aattagtaat
    27481 aatccaatta gaactaccaa caagaatgct actaaaccag cccaaaatgc ctgtcgtgcc
    27541 gtcaataggc cgttctggat cgtgcgactt ccaccagaga atggagtaaa atcgagatta
    27601 ttgtcatcac agcccgtcag gtgatcgtaa taatcattaa aaatatttgc accaaagtgg
    27661 gcaaaaatcg ttcctaataa agccagtaaa ccggaaataa aattaattct accggtctca
    27721 taccaaccta cagaagtacc taaaattacc ggcgcaacgc tggcagtcag aaaaggagcc
    27781 cgatatactc ttaaccataa ttttagagaa tccgataatt ttcgattagc tgacatcatt
    27841 acctaaatcg gtcaaagatt tctctgaagg aactcaagaa aagtctcgta catatgaatt
    27901 tgcccttcac ggtcggttat tccatgtttt cttccaggat aaagcatcaa ctcaaaacgg
    27961 acattgttgc tgattagttc atcaataaat tccagacttt gctgtaagtg gacattatca
    28021 tcataagtgc catgaattag taaaaaatta gcttttatct gactaacata attaagcggc
    28081 gcactttctt tataaatatt ttgattatta atgggcaatc ctaaatatcg ctcagtatat
    28141 ggggcatcat aatacttcca atccgttacc ggagaaatag caactccggt tttaaaaact
    28201 tccggcgctt tggtaagagc atacaaagtc atatatccgc cataactggc accccagatt
    28261 ccgattcttc ttgaatcaat atacggcaga ctttttaaat atttcacccc atccagttga
    28321 tcctgtaatt ctattttccc cagttgctgg taaatcctgc gttcccaata tcgtccacgc
    28381 ccatacgaac cgcgattgtc gaggctgaaa attaaataac cctgttgcgc taacaattgg
    28441 gtccaggcac ctccatagcg gttttgaacg acttgcactc caggaccacc atagacataa
    28501 acaataaccg gatatttttt ttgcggatca aaatcactcg gatataatag gctagcataa
    28561 agtgaatccc catcaattgc cggtatcttg ataaaacgtg taacgccgaa attatatttt
    28621 tgcggactga aatctttgtt atgatataag tccgttattt ttctaccatt attttgaata
    28681 attctgacat ctacttgcga gttaacattt gagaaaaaat caagataata tttcaaatcc
    28741 ggtgaaaaaa ctgccgagtg cactccatca ccggtatcaa tctggctaat ggcaccggag
    28801 ccaatatgca cgctataaat atgccgttcg agtaccgaat ttttagtcgc tgtgaaataa
    28861 accagactgt ctttttcgtt tacgccattc aattcggtaa ccatccaatc gccgctagta
    28921 atcggtttga tcaaattgcc ttcgaaatca tagagataaa gatgcatgaa attgtctatt
    28981 tccgaataat agataaaatt gcgcgaattt ttcataaaat agtataagtc cgtgatattt
    29041 aaccaatacg gatctttttc ttcgagaata acttttgtct ggccggttcg cggattacca
    29101 taggtaactt tctggcaatt ttgaagccga ttaattgatt gaataaccac caaactggtg
    29161 tcagccaacc aatctatccg gacaacgtat tcattcttgg attgaaaatt attcatacaa
    29221 acaatttttt gggtctctat atcaactgtg aatactttga cttctggtat tttctcgcct
    29281 gcattcggat aatattgcca ctcgatttct ggatgcatag gagaccaatc taccagcggg
    29341 tagcgactga caccattttc atttaactgg caataggcaa tcattttact gtccggcgac
    29401 cactgatacg cttgttcaga atcaaattcc tccgcataaa cccagtcggg taaaccatta
    29461 aaaatcgatt cactgccatc agttgtcagc cggatcgtac tttttgattc acagttgaca
    29521 atccagatat tatggtcgca aatatagctg acccatttcc cgttgggaga gaattttgga
    29581 acactttttt gggttccgtc atttgtcaaa cgcactatag ttccatcgcg ttcatcgcct
    29641 aggaaaaggt cattctcacc agtcagtaaa tatcggaatc catcgggagc cgatatgaaa
    29701 ccgaacggat caagttgaac cgtgtgagta tcgttactcc accttaattg ttcataagta
    29761 aaaaccgtgt caattttaag ggaattaatc gacaatttca gcaaatcgca attttgattt
    29821 ttattttttt cgagaaataa tactccgtcg cccttcggag tccaggtaaa ttgtttctgc
    29881 cggtaagttc tcgcatccaa tcccggatca tcaatgattt cccgcacggt taatttcttg
    29941 gagttctcag ccaaaattac tgaagtactt agcagaaaac ataaacaaat ccggaatatc
    30001 ctcatttcat tttcctattc taaaaaactt gcggacagcg gctaacaatt catcttcgcc
    30061 aataattgaa atcggctcga ccggtaacga ttttttgaag tagcggccat aggctttttt
    30121 atcaatgcga ctgtcaagat taataacgac tcccacatca ttaatcgagc gaattaagcg
    30181 accaactccc tggcgaaact tcaagaccga ttccgggaca tagtattcgt taaatggatc
    30241 ttgcccctcg gctttcaact tttcgacatt agcttcgata attggttccg aaggcaccgg
    30301 aaatggtaac ttggtgataa ctaaaatttc caatgccggt ccaaccacat ccactccttc
    30361 ccaaaaacta tcggtaccca gtaaaaccga attctgctct aagcgaaatt gctcgagaag
    30421 cgcggtcctc gaggcgccct gtccttgcgc taaaagagta acacccattt cgataaaagt
    30481 cggcagacat agttgataaa cctcgtacat aaaattatag gaagtgaaca aaaccagaat
    30541 gcccttcccg gtttcgctgg aaaggcgttt tagcaaccgc gcggctataa atgcttcact
    30601 aatttcatta tcgttctggt gataggtaaa aaaagccatc tggtcgttat aataaaaagg
    30661 tgaaccgagc gcgcgactta caacattatg atcttctatc aaattcaaac cggtccgact
    30721 aagaacataa tcaaaagctc cggcaacttg caaagtcgcc gaggtcagca tcgcactgga
    30781 aacattctta tagacctttt cgaaaatgtc gttcgcaata ttgaggggag tgcaaacaat
    30841 ttctacagta ttgtttttac cattgggggc aatttcatac cagaaaatat gcgaatcgtc
    30901 gttggcagtt gtaactttac gcaacgtttc cagaaatgcc tcgaccataa ttttctgatt
    30961 cattagccgc acttgcaatt catcatactc ttcggcataa tcgccccata tttcgtccat
    31021 tttattgctc aaattaccca gcagattcca caaagtgccc agttcgtaga tcagattatt
    31081 cgtttcgatt tccagattgg gaaattcacg ggaaaaatct ttatagcgtt ttttaatgcc
    31141 aaaagacttt tgattgccat tagccggatt gaatttagtg gcaacgattt ttcggaaaaa
    31201 tacctccgct gcaattttga catcggaggt tttctcgaga atcatttgac ggagcggttc
    31261 gattttatcg agacttttca gctgatgagc gctttcggct aaatctacca gcaagccacg
    31321 ttcaggtgcc gagccgctat agattccgtt tagaaaataa ataagcgttg gtaggttgac
    31381 tttttgcgca aaataattat aggaattctt ttccagatta tgagcttcat ctataatcaa
    31441 agcatcaaat ccaggtagaa tctggttatc ggcggctgag tcggcaagaa gcagggaatg
    31501 attaactacc ataatgtcgg cattcatcgc atgataacgt attttgccca gaaaacagcc
    31561 gccatatttc tggcaaacac tggtagtaca atagccgggc tcggaacaaa tttcgttcca
    31621 gatatagtaa tgcgccgcca gattaaatcc attattttct gcaatgtcgc cggtgcgagt
    31681 atgcttaagc caaattatga ttggaataat ggcggagcgc gccgccaggt gaagctgttg
    31741 aggcagagca tttagataac gatgccagcg cgtcaagcag atataatttg aacgtccctt
    31801 aagtaaaacg gctttgaatg gcagtttcaa tttttccgat acaaagggaa tttccttata
    31861 aaatatttgt tcctggagag tcttggtgtt actggcgatt actacccgct ttttctcctt
    31921 cttctgttga ttcaaccaaa ccaccgccgg tattaggtaa gccagtgatt taccgactcc
    31981 ggttccggct tcgataatgg ctgaatcgcc tgcctgcaaa ctgttaagca ccaattgcgc
    32041 catttccttt tgttgtggtc ggtcttcaaa accaacgaag ttctgggcaa ttggtccagc
    32101 cggttcaaaa aacttctcaa tttttaagga agcctttgcg ctttctttat ttttttcaat
    32161 agactgacca aaaatattta ctgggggctc ccaaccgatt tgaggtgctt ctgcagatcc
    32221 cagagttttc ttcaataata ggtcttcagc ggcgtgcttg taaagcagtt tatttgggtc
    32281 ttcggtgtct tgcaacacca tattgatagt ctggatcgtc tccagatcgt agcttcgcac
    32341 ctcttcgatt aatttgagaa aaatcaaacc ggtattgaga gcatcattct ccgctcggtg
    32401 tgcgcccttg gcacttaatc cataaaactc agcgatagtg gacaagcgat gattatataa
    32461 aaaaaagaga aaagcctgcg ccagaagtaa ggtatcgtat aacggattat aaagaatcct
    32521 tttcctctga cttttgaaat ttttcagata ttcttttaaa aaaccgagat caaaaccgat
    32581 attatgtccg accagtggaa ttctaccgat gaactccagg acttcatcaa gatgttgttc
    32641 aaaaggcaac ttatctttta gcatttcatc tttgatgccg gtcagttgct cgatttccgg
    32701 agagatggaa aaaccgggat tacagagaaa attcaaggta ttggacggct taccgtttac
    32761 aaattttact gccgcaaatt caatcacggc atcttgttgc ggattcaagc cggtcgtctc
    32821 aacatcgagg gcgataaatt cgtttaatcc ggcgagagtt aaaaaatcgt ccattagatc
    32881 atttgagcaa ataatttgat cgcctcaagc aataaagctg gtttattacg taaagcaatt
    32941 ttgaaaacgg tcatgatgtt aatatcgtcc ggcgggattt tttggagcaa agtagcaatt
    33001 tcatctagtt catcgtcact aagttcagtc atccattctt ttattttata aaagcgcgca
    33061 taatctttgc cgacattttt atcccattcc gtctgatatt ctttcaatac atttacgctc
    33121 cagtcattgc gggcgatcgc ttgagcggcg accaaaccgg caattttacc ggctgtaatt
    33181 gccgccaaga ttccaccgcc ggttaccgga ttgacctgcc gggcagcatc tccgacaatt
    33241 agtaatccat ctgaggcgat tttttttagc gatttggcga cgggaactcc tccggcgacc
    33301 gtggttaatc gcgccgcctc aggaaatttt tcattgataa aatcatccaa atattcaatc
    33361 gccgagcgtc ccttataata agcaccgttg acacccagac cgacattggc ggtgtggttg
    33421 cctttgggaa atacccaggc gtaaccaccc ggcgcccagc ggctggaaaa ataaaaatct
    33481 atttgattct cagctacatc cagattgcca agtaaaacct gggcacagga ttcaatatct
    33541 tttaaaacgg tctgagtgcg gattccggca aagcgggcaa ttcgcgattc caccccatca
    33601 gccgctatga ttatcttaga tttcacgata taatcgcggt cgaggtggcg cacaaacact
    33661 tcccagttct gctccccatc tgatacaatt ttataaacat tagccttagt gaatatttca
    33721 actccttgag tagcggcgat ttcggctaat ccattatcga ataaacggcg gttaagaatt
    33781 acgccatcat taggcaaatt caaccagacc ttttggtcgg atggagaaat taagcgggct
    33841 ttggtaatgg tagtcgctac ccagcgcgga tcaattttta cgaatcgttc gagatccgga
    33901 atcgaggtgg cttcggcaca tcgaaccgga ataccaattt cccgatctct ttcaaaaaga
    33961 gcaactgtat tagtagccgt tttagcgaaa cgagccgccg tactaccggc agggccgcca
    34021 ccgacgacaa tcacatcata ttcttttcgc ataatttttc ctattcaacc agccttagcg
    34081 ctttaaatgg gcagactctc acacaaatct gacaaacatt acattgggaa tcatcaacta
    34141 tcaggcgcga ttcagtcaat gtgatacaat ccttcggaca taccgaaata cacgtcccac
    34201 ataaatcgca acagtctggg ttaacttcta tcattccgat tcctcaactt gaaacgttgg
    34261 ataattcaga ttaaatcgag ataaataata aaatcggcaa agataatagt ttatcaatag
    34321 tacaataaaa aataggggga actcgacgca taacgtcaaa gccaggaaca agatcggata
    34381 gcggatagaa cgcaagaccc aggcttcagt tggtttgaaa agcagaatga tgaaaagtgg
    34441 cgatgaaatc aaacaggata gacttataac cggatcttta aaatacatac cgagcgcaaa
    34501 gctcaaagcc agacaaatga atgccaacca aatcgtaact ctattttcat aatgaaccga
    34561 gatggtcttc ttccctgttt tgagatcgcc ttctttatcc ggaatagtag tcaaaaccgc
    34621 taccgccgtc caggccaata aatagggtat cgcataaata atgtctttca attgccattt
    34681 accggcgatg atccagccgc aaataaaaag acacaatccg ctgataaaat tgaccagcac
    34741 gcccaacatc ggcttgtctt tccattcaaa tggcttataa ttatacaaat aaccccagaa
    34801 aaaaacacaa attcccaacg caataaagaa ctgccagttt attatcaagg ctaataccaa
    34861 tgaaacaccg atggttatca gtgcaatttt caaggctaaa tcagctttga tatgttcttc
    34921 tgaaattaaa aaaagtttac ggttttcttt atcgctttcg atatcgtgca gttgatttaa
    34981 gataaaagtc gctcccgtcg ccattgtaac aactataaaa cctaatacca gtttccagtt
    35041 gacttctaat gaccaccagg ctggttgctt cacgctgaaa ataaaatagc cgctgtaacc
    35101 ggcaagtgtg ataatccaga ccgcaaagaa taaggtcggt cggaaaataa acaggtaatc
    35161 aagccaggca attgatttac tcggaacttt taacatgagt tctccttctc ttggttaatt
    35221 ttttcacagt aatggccggt aaaacaagct gtacaaaaat tttcagccgg taagctcatc
    35281 gcatttaaca aaccttccgg gcttatatat tcaaggctgt caacctctaa atagtccttt
    35341 atttctgcaa tggatttctg attggcgatt aattcttcgt gcgacggaaa atccatacca
    35401 taataacaag gaaatttgac tggcggagaa ccaatacgga tatgaatttc tttagctccg
    35461 gcggaccgca acattcgcgc caatttacgt aaagtcgtac cgcgcacaat cgaatcatca
    35521 acgacgacaa ttttgcgatc ttttaatact cccccgaccg ggttgaattt aattttagct
    35581 gtaaaatcac gcatagtctg ggtgggatgg ataaaagtcc gtccgatata atgattccga
    35641 atcaaggcaa tttcaaaccg agccgttgaa cgctgagaat atccgagcgc aatagtatta
    35701 ctcgagtccg gtaccgaaat cacgatctca gcttccgggg cgggcttttc catagctaaa
    35761 attttaccca atttgcgcct ggttttatca acatattcgc caaatatctg gctatccgga
    35821 cgtgaaaaat acacatactc aaaaatacaa tgcttcggct caggtggagc gatttgatag
    35881 gatttaacat tattaccgtc caatactatc atttcgcccg gtttgacatc tcgctcataa
    35941 gtagcaccga ttaaatccaa agcacaagtc tcggaagcaa aaatgtatga accatcgaga
    36001 gttccaatgc ataacggccg gaatccgcga tgatcacgag ccactatcat tttgtcgtca
    36061 aacaaaatta aaaccgagaa agcacccttt atccgacgga aaacctgtaa aaatttctcg
    36121 actaacgaat caccgcccga atgagcaaat aaatgtatta tatattcggt gtctgaggtc
    36181 gtttgaaaca aagccccatg actctccaaa tctcggccga tttcatccag gttaatcagg
    36241 ttgccattgt gaacgatgct aatctgactg cctttgtact tgaatgctaa tggttgaatg
    36301 ttttcgttac cattgcttcc ataggttgaa tatcggacat gtccaatagc cgtcttcccc
    36361 gataaatgca taagttgctc acgattgata aaaacatcgg cgaccagtcc tttgcctttc
    36421 tgaagatgac tgactttacc gtcgaacgaa gcgataccgg ctccctcctg accgcgatgc
    36481 tgtaaagcat aaagtccgag gtaagttaga gtagcggctt cttcatgatt ataaattcct
    36541 acaattccac acatttttca tccattaatt agttttatgt gatttgaata tatccaacca
    36601 cgctgattgc gtttggcttc gatgcgataa atgccttctg gtatatcaat cgccagcttt
    36661 gtagtcgttt ccgtgtggta aattttacca ttgcgataaa ccttacattc cgccttttgc
    36721 ggtaaaaaaa catacaaggt gccaccggta gccattccgg tgtcacccat ctgcagttcc
    36781 gtctggtcag tctgaacata aaaacgaaat cctcgagcat caccacgccg aaagttagaa
    36841 tagaaacagc gtcctgattt tattatctta attatttctg cttcagtatc agaatcattt
    36901 aattctgtaa ttttgcgctc actcaaaagg tgagtacgga tggatttgaa cattatttta
    36961 taatcgaaaa tggcttttgg aaaaacgccg aaaaccttta ctttcgtagc atgagcatcc
    37021 accgaaccga ttcccactac gttacgctta agattgatat tatcccacca ctttaaaagt
    37081 tcatcggccg gactggttga aaaactgcgc ggatgaatga cattccagta cttattctta
    37141 atcgtagtcg cttccagcca gtgcgacatc atattccaga tttctaccac ttctatttcg
    37201 ggataatcca gagtgctcca ggtgatagcc ggaaacccgg gtaatgaata attcttatca
    37261 cgttgttcca gcggatgcgc ggcaattccc agagcaccag cctccttcac cgccttaata
    37321 tattgctgat gagtcaaatc agtaggtaag gttttatcca taccaaacac taaataatga
    37381 tggcgatctt ctaaatcctg aatttcataa ccaatgctaa caaacaaatt atcatgccag
    37441 ccggcatatc cttcatctcg accgcgcaaa gtttgatggt cgctaaccat tatataatcc
    37501 aatccggctt cctgagcggc tcgaataact tcttcatacg tcccgctccc atcggaatag
    37561 acggtatgaa tatgaataca accaatatat tcgttcaagg ttttcattca attccgttaa
    37621 taaacaatat cgcggcgcca gcacccagaa tatcagccac aaggtcacgc caactgaagt
    37681 aattttcctt ttgttttaaa tcccttattt ctttcaataa tcctaaagaa atcgtcaagc
    37741 cggcggcgtt tcgattagcc cggtcagtct ttaaatcaaa aatgcggttt tgagtataaa
    37801 aaagagaagt gctcatataa aacgaagtac taaaatgttt cagtttgtcc attgcaaacc
    37861 atttatcatc cggctcactt ttgattattt ctccgaccgg tggagcggct aaaagcctac
    37921 cgccaattgc aaatatcaat aacaatagcc accaattttt catattattc cgagtgaact
    37981 taataaaacc attgcaatca aaaccggcgc tacataccga attaaaaatc gccagactgg
    38041 aaaaaaccaa gctggagttc cttcagctcc ttgccgcatt tctaacaaag cattattctt
    38101 gccccaaaac cagccaacaa atatagttat aaataaacca cctatcggca aacaaatatt
    38161 agaggctaaa taatcacaaa aattgaaaat ggaaaggttc ataatctgga aactcgacca
    38221 gcgaccaaag gagagagcac tgggaattcc aagtaataaa cttatggcac ctccgataat
    38281 tgctatcatt ttccgattta acttaaattc atccacaaaa taagccgtga tgacttccag
    38341 taaagaaatc gtcgaggtca aggcggctaa ggctaataag ataaagaaaa gaatccgaaa
    38401 ccagtatcca cccggaatgt aattaaataa aatcggcgaa gtattgaata ataaacttgg
    38461 tccctggtga ggttttagcc ctagcgcaaa aattgcggga aaaatcataa atccggctaa
    38521 aagcgaaaca gatgtatcaa ttaccacaat ccgggttgct tgcgacggga tattattttc
    38581 ttttgataga tagctaccgt aagtaatcat cacccccata ccaatactaa gtgaaaaaaa
    38641 tgcctgcccg agagccacta aaaccgttcg accggtgaca ttggcaaagt tcggtttcaa
    38701 caaatagaga atcccagcct ttgaattctc taaggtaatt cctctaacta ccaatatcaa
    38761 aatgattata atcaaactgg gcatcataat tttactggat ttctcaatgc cacgagtaac
    38821 cccaaaatat acaatgccta ccgtcagcgc cataaaaatt gtcaagtaag gtacactcca
    38881 actgccactg ccggataatc tactaaataa tgctaccgcc tgcgcggtat ccgttatcgt
    38941 ttgaaatgaa cctttgacgg cttcgacaat atatcccaga caccagccgg caatcacatt
    39001 ataatatgag aggataataa agccagtaaa aactccgaga aaacctactc ccttccagaa
    39061 gggattgtta tttgatagtt ttttaaaact accgactgga tctttctgag catttcgtcc
    39121 aattacaact tcgctaataa tgacgggtaa gccgataacc gcaacacaaa tcaaatagat
    39181 gaaaataaat actgcaccgc cattctcacc gaccaaataa ggaaatctcc aaatgtttcc
    39241 cagtcctaca gccgaaccag cggccgcaag tacaaatcca atttttgagc cccattgttc
    39301 acgcgactta atcatcttac aaattcaccc tgactgataa atttagtccg atttcagtgc
    39361 tacccagttc aatctctccc ttagggaaat ttatcaaatg ggcatcaaca acagcatcca
    39421 gtaaacaaaa aatgtaagtg cctaaactcc accagccata tttattacgc ttttctctgg
    39481 gacgccagga attcagcgta agttcgtaac cggtaaccgc cagcacttgg gcttttttct
    39541 cttcttcggt caaccctacc caggtttcga ttcccagcgt ttccttcgtc gttttgacat
    39601 agtcataaat ttgattataa tagaaaaaat tatagaggaa atatccctcc gctgacaata
    39661 gtactaaacc tttgaccggt ttcttattat agacttgtcc ccagccggga attattgccg
    39721 aacggataaa tgcccctttc ggtgaaattt tcataagtgt atcagctggg ctagcacttt
    39781 gcaaattgct cttcaatatt attaataatc ctgccaccag aaaaatacgc ttcaaatcca
    39841 gcttttaatc ctattaccaa gcccctacga ctgaagccga ttgaggaact attttgatat
    39901 caacttctcc ggcaccggca tcgaatattt ccccgtcgaa atgcaccgga agtggttttt
    39961 cactggaaat attaattgct tgacaacgca tcgtcaaaac tcctggaact tccgatattg
    40021 tgccgtcttt tagccttctt agattcagga ataatttcca gaccggcatt tccggaatca
    40081 ggcatacatc caacaacgaa tcatgaatac ttgccagcgg atttaatttc agaccaccgc
    40141 cacaagccca gccattgccg accgctacca aaaaggaacg atcggtgctt atcggacagc
    40201 cgtcaatttc gattgccaac ggaagcgact tccagaatgc caaactcgat agcaccgagt
    40261 atagataagc catactgcct ttcaacattt taaggctctt ggttttcaca ttaacgtagg
    40321 cgtcaaaacc cactcccacc ccattgataa aaacacgatt gccgattgtc ccgaggtcca
    40381 ttaagcgcgt tttatattca tctaaaatct ggatggctct ttcaaattta tgcggcacat
    40441 tgatacaacg agcgaagtca tttcctgttc cttctggaat cacgccgatc ggtgtttcag
    40501 ttcccgccag accgttcatt atttcattag ccgtcccatc tccgccatag gctacaacgg
    40561 catcatattc ctgatgaatc cggtcagcaa ttttagtagc atgaagcggt ccttcggtaa
    40621 taaaataatc gaagcgcgat ttggttttag ccgcgatttc tttcaaaagc ggcaggttgt
    40681 gaattgtctt ccctttcccg gcaaccggat ttacgataac ggcaaatttg tacggcaatt
    40741 attttcccct tttaacggct atggcttcaa tctcaaccaa cccatccagt ggcaagcgac
    40801 taacctgcac ggctgaccga gctggcgctg attccggaaa atattcgcca aaaacctgat
    40861 ttaatagccc aaaattatct aaatcggtta caaataccgt gaatttcacc acatttttca
    40921 aatcgctccc agccgcctgt aaaatcgctt ttaggttgcg tattatttga cgcacttgct
    40981 gttcaaaatc attagagaca atttttccgg cgactgggtc gagtcctaat tgtcccgccg
    41041 taaatacaaa atttccggaa attacggctt gactataagg tccaatggca cgcggcgcat
    41101 ccgtagtatt gacaatttgg cgctctactt cccccagcat atgtttaact ccttattggc
    41161 ttttaattca tctaaacgtc tgaccggcgt cgtccaggga gcattacgta atttttcagg
    41221 ttcattagtt gcctcctgat ctatttttac cagggcttcg atgaagcgat ccagagtttc
    41281 cttgctctcg gtttcagttg gctcaatcat taaagcctct ttgacaatca gtggaaagta
    41341 aaccgtcgga gaatgaaaac catagtccag caaacgcttt gctatatcaa gtgttttaat
    41401 accagaacgt tgtaactggc gttctccgga agcgacaaat tcatgcatac agattcgatt
    41461 ataaggaatt tcaaatacag aactcaattt ttggcgcagg taattagcat tcaagatggc
    41521 gttgcgcgaa acggcttgca agccttttgt acccatcatc cgaatgtaac agtatgcccg
    41581 gacgattacc ccgaaatttc cataaaatcc aagtactttt ccaattgtac gcggataact
    41641 ataatttagt gaaaatctat caccattttt ctcaatgcgc ggtaccggga gaaattcgac
    41701 cagtttttca tttacggcaa ttgcgccgct accaggtccg ccgccaccgt gaggcgtcgc
    41761 aaaggtttta tgtaaattga tgtgagtaat gtcaaagccc atatcgcccg gccgcacaat
    41821 accaagcagg gcgttcagat tggcaccgtc catatagacc aaaccaccct gattatgaac
    41881 tatttcatta atttcgccta cttcctcctc gaagagcccg agagtgttgg gattggtcag
    41941 cattaaacca gctaccgaga aattcatttt ggcgcgcaag tctcctaaat caatacaacc
    42001 gcgttgattg gatttcaatg aaatagtttt ataattagcc gatgccgcac tggccggatt
    42061 ggttccatgc gccgagtcag gaatcaagat aaactggcga ttttctcctt tctgatcaaa
    42121 atatttcttc ataagcaaaa caccggtcaa ttcgccatgc gagccagccg ctggttgaag
    42181 tgtggcggct tgtaagccgg taattgcaca caaatatcgt tccagttcgt aaataatctg
    42241 cagagcgccc tgaaccgtgc cgacatcctg gagaggatga cagttagcca gtccggaaag
    42301 agcggcactc cgatcattga ctttcgggtt gtacttcatc gtacaggaac ctagcggata
    42361 aaggtctttg tcaacgtgat gattcattgt tgataaattg gtgaaatggc gcacaacttc
    42421 cggctcgctg acttccggca attgtagttc gctctggcgc agaagttcgg ctggaattaa
    42481 ttcggcggga gaaactgccg attcaaaacc ttgtggcaaa gagacgcctt ctttaccaga
    42541 ttgagaatac tcataaatta atttctccat cctatattcc ttttaccttt tagactcgat
    42601 ttttgactta attaatggaa taaccgcttg cagagcttca ggaattttgt cggtaaatcg
    42661 gccaccggcg gtcgccagat gcgcctgacc gccgccaccg ccacctaaaa ttttccccaa
    42721 ttcccgaaca atatcgccgg cttttaatcc atattgtttg attgccaggt cgctgaccac
    42781 aacgacaata ctcggctttt catcaattac accggcaatt acaccgatgc cgttatgcaa
    42841 tttagcgcgc accagatcgc cgatttcttt taattcagcc agacttttga cattcgcttg
    42901 ataagtaaaa acctttaatt ccccaatcat ctcggctttt tctaagagag tatcaatctc
    42961 ctgttcaaga tccgctttat tgtttttatg aaattgtttt tccaggctct tatattcggt
    43021 taataatttt tcaattcgat gaggtaactg ttcacggctg acgccgagca aggtcgtgat
    43081 ctcttccagc aggcgttcat tctcccgcgc taattttaac accgattcgc cagtgatagc
    43141 ttcaatccga cggacaccag cggcaatcgc cgtctcgcta ataattttga aacagcctat
    43201 atcaccggtt cgattgacgt gtttaccgcc gcacagctct tttgaacaat caccaacgtt
    43261 gattactcgc acgacatcgc catacttttc gccaaacaac gccatcgcac ccgattcacg
    43321 agcactctga taatccttca cttcagcatt caccggcaaa ttttctcgga tgcgggcatt
    43381 aacccaatct tcaactgcct ctatttctga cttggtcatt ttagtaaaat gggtaaaatc
    43441 aaaacgcaat ttttccggtg tcaccagcga accggattga tggacgtgct cacccagaat
    43501 ctgacgtaaa gccgcctgta aaagatgcgt ggcagtgtga ttggcgcgaa tctgcaaatt
    43561 agcgacgttg tccacgctgg ctttgaccat cggattatca attaaattcc ctttttcaaa
    43621 atgaccgcta tggataatgg aagagccaat tttccgcgtg ttgtcaatcc tgattctgaa
    43681 attattcccc gcgataattc cgacatcgcc gacttcgccc ccagcctcag cgtaaaacgg
    43741 agtccggtcg agaatcaaat gaactttctg accttcgatc aggtacttgc gaatcaccga
    43801 ttgcgtttcc agattttcat agccgacaaa ctcagaatcg tcgccagtcg tcagcgtttg
    43861 ccaatcttca ttttccagaa aatcatcggc ttgaaacact accgaagccc gagcacgttc
    43921 tttctggcgg ttcattaagt tttcaaatcc cttttcatca atgctaaatc cggcttcctc
    43981 agccaatagg cgcgtcaaat ccatcgggaa accaaatgta tcgtagagtt taaaaacttc
    44041 ttcaccagga attattctga tattatttcg ttgcaggctt tccttgagtt tagcaaagac
    44101 ctccaatcca cggtcaagag tcaaaccaaa tgacttctcc tcggctttaa taactgcttg
    44161 aatatgatta cgccgttctg caatttcagg gtaagtcgca ccgagcacct caatcacact
    44221 atcaactaac tgataaataa aaggcttatg catttccagc attcgaccga aacgcgctgc
    44281 ccggcgcaaa atgcggcgaa ttacataacc gcggccatca ttggacggca aaccgccatc
    44341 cgcaatcgca aacgtcagca ttcgaacgtg gtcggcaata acgcgatggg gcacaccgtt
    44401 ttccgtatga tattctttgc cggttaaaga tacaatccgg tcaatgatcg gcttaaataa
    44461 atcggtatca taatttgact ttttcccttg caaaacagct accatccgtt ctagtccggc
    44521 tccggtatca acgtgcttaa gtggcagagg cttcaactct cccgatttat cacgatcaaa
    44581 ctggataaaa accagattcc agagttcgat atattccggg ttgccggaat taatcatttc
    44641 cgcttgctgg tgctccggat ggggaccggt gtaataatga atttcagaac aaggaccgca
    44701 gggaccggtt tcgcccatct cccaaaaatt gtccttttcg ccaaagcgaa gaatcctact
    44761 ctggtcgagt ccggctattc tctgccaggc ttgaagcgcc tcttcatcat cttgataaat
    44821 agtcacccac aatctttccg tcggcaattt ccagacttcc gtcaacaatt cccacgccat
    44881 ttgcacggct tccgctttat aataatcacc aaatgaccaa ttacccaaca tctcgaaaaa
    44941 agtgtggtga taagtgtctt tccccacttc ttccagatcg ttatgtttgc cgctaacccg
    45001 gatacatttc tgtgaatttg cgactctcgg gctttcgatt ttctcctgcc ctaaaaatat
    45061 gcctttgaat tggttcattc cggcgttagt aaatagcaaa gtcgggtcag attgcggaat
    45121 aaccggggca ctacgaataa ttttatgacc gcggctagcg aaaaattcta aaaactcacg
    45181 acggacgtcg ttggcggttt tcaattatac tcctgtaaac tttataaatg caaaaaatca
    45241 tatttcaaat ttactcttca ttaagcataa atatgaaggt gaaagttatc tggaatcggg
    45301 cttataatca agcagatatt ggcgaaggca aactttaagc actttatttc tatagaattt
    45361 tttgcagtac tttgataacc acatcgggct tatatccttt ttgaattagg taacgatata
    45421 acttatcgcg acgcttcgga tcgtttttga gagttgaggc ttttttttgt ccaatgatcc
    45481 aagccgcctt caattgatct tcatctttca ctaagttttt aatcgcctca tcaatcacct
    45541 cacgatctat ccctttcgca accagagcct gcctaatttt ccgaattccg ttatgattca
    45601 attccagttg atcacgaata aaggtttcgc aaaaaacttt atcatccaga tagcctcttt
    45661 ccgtacaata cttgattacc cggctagcca ccatcaaagg atatttctgg cgacgggtaa
    45721 gatataattc gagttccctt tgactgcgta ttctaagtcc cagatatcta attgccgctt
    45781 ctttcgcctg ttcgaatagt tcgtgttgct gaatcatatt aatctgcttt tccgtcaatt
    45841 gctgaccaac gcaaatccga aaaaggttaa gagtttcagc actaatgccg aaagcaaatt
    45901 cattatcgag ataaatatta atgcgcgccg acttcttctt ttgcgattcg atcttggtta
    45961 caacttttaa tatctcggct tcggacaaaa agtctgagga cattttattt ctttctggat
    46021 taaaaggaat ttattccttg attagcccta gataagtttt aacctgctgt tcgagttttg
    46081 taagcaattc tttattttcg gtcaggtaac ctttagcgct ttcgcgtccc tgaccaatac
    46141 gttcatctcc atacgaatac caggctccac ttttttggac aaacccagcc tcgacggccg
    46201 tatccagaat atccccaaca tacgaaatgc cctgatcgaa agtcaagtca aattcagttt
    46261 ctttaaatgg tggcgccatt ttatttttaa ccaccttgac ccgcgtgcga cttccaacat
    46321 tagtgttacc ttctttgatc gtggaaattc ttcgaatttc aagacggaca gaggtataaa
    46381 atttcaaagc ccgaccaccg gtggtcgtct caggattacc atacgtcacc ccaatctttt
    46441 cccgaatttg gttaatgaaa atcaccgccg tgttggattt gctgactgca ccggtcaatt
    46501 tacgaagagc ttgagacatc aagcgcgctt gtaatccgac atgcgaatcg cccatttccc
    46561 cttccaattc ggcgcgcggc accagagcgg ctaccgaatc aatcacaatc acatcaatag
    46621 cattactacg aaccagagtt tcagtgattt ccagcgcctg ttcaccgtgg tctggttgcg
    46681 aaatcagtaa atcgcgggta tttactccca gtttagcggc atattggggg tcgagcgcat
    46741 gctcggcatc aataaaagcc gcataaccac cggctttctg agcctgagcg atgatatgca
    46801 aagccagcgt cgttttcccg gacgattccg gtccaaatat ctcggtgatt cgaccacgcg
    46861 gaataccgcc aaccccgata caagcatcca aagataagca accggtcgga attacatcca
    46921 cggtgatttt catcgagtca tctccgagac gcataatcga acccttgcca aactgacggt
    46981 caatctgggt tatggctaaa tcgagagctt ttattttatc atcatttttt tccgccatct
    47041 ttctctcctt attttatttt aaattaaaca cggccaggcg tgtgtaaatt ggtcctccca
    47101 ctgtcagatt actctcatag aaaattacat cctttaccgg atttatggta ggatttattt
    47161 tataatttaa aaaactttcc agtccggcaa cctttttaaa aggattttta acccgcccga
    47221 tggtcaaatg tggtctaaat tcgcgttttt ccggttgata accgaaattt tctaaaacca
    47281 attcaattgc ccgagtcaat ttttgaattt gctctaatcc ctgaccgact cccagccata
    47341 atacgcgagg cttgtggtta ttaggaaaac acccgagccc ttcccaaata aattcaaatc
    47401 tatcaatggt agtgatgcta ctatctaatg cctgagaaat agtcgggata actttttcat
    47461 cggtatcgcc cagaaatttc aacgtcaaat gcaaattatt ggtaggtacc aggcgcgtat
    47521 tttcacgtaa ttccggaata gtcctttgga ttgtatccac ctgcaagcaa gtttcctgac
    47581 taaccggtat ggcaatgaaa gttcttttca tcttttattt acctattaaa tataaacgca
    47641 gttgatttaa cgccgcctga gaaaatagta acttattagt caagcgatcg ccactaaatt
    47701 ggtattgaaa agtccgagta gcgtttttgt cggaaacccc aatccagacc aatccaaccg
    47761 gtttggcatc actaccacca gagggaccgg ctatcccggt ggaactcaaa ccaaggtcac
    47821 aaccggaaat ctgtcgcact cgggctgcca tttgattggc aacttccgaa ctcaccgcgc
    47881 cgtatttttc gataatttcc ggtttaactc caattaaatt aattttcgtg gcattgctgt
    47941 aggcaacgat tccagtttta aaataattcg aactaccggg aacatcagtt aaacgactgg
    48001 caatcagacc gccggtacag gattcggcta ccgcgaccat cagatgcttc tgtaataaaa
    48061 gattagcgat aactgattcg agagtatcat catcaaaacc aaagacgtaa tcgccgagaa
    48121 tttctgcaat ctccttgaca tactcatcta ttttagcact ttcctgaggt tcagaaatcg
    48181 tcagttcgag gtcaatatgc gccgtctgcg gtaggaaagc tactttgacg tgaggatgtt
    48241 gttcaatcca ttcttttaat aatgtataaa tctgagattc aggtattccg gtaatgtgca
    48301 ggtacttttt tacaatagct ttatgaccga gagcggctaa ttgcgaagcg attattttgg
    48361 tcattatcgc ttctacttct ctggggacac ccggcaaaac ataataaacc cgattttgat
    48421 gttcaatttt caagccggga gctgaaccaa ccggattatc cagaataaca gcttcctttg
    48481 gtaggtaggc ttgtttcaga tttatttctg gcatctttag tccccgtttt gcaaaatgct
    48541 tcactaaatt actggctgtt tctttattaa atacaagcgg cagattaaaa tagcgagcga
    48601 gggcagaaac tgttatgtca tcattcgtag gacctaaacc gccggtgatc attaccaaat
    48661 tgatttccgg aggtaaatta tcgagaactg tccagatatg tttctgttca tcgcctacgg
    48721 ttacaattaa gctaaccaca ataccaaaat tatttaatgt ccgagcgata taggcagagt
    48781 tggtgttatt aacgagtccg gagattagtt cattcccgat cgaaatgata gccgcattgg
    48841 tcacaggatc agaattccca aattgacgat aataccggta aaaataccag ccaggatatc
    48901 gtctgccatt ataccaaagc caccaccgat caactccaat ttgcgaattg gtttgaattt
    48961 tttaatatca aatattcgaa aaataagaaa cactaaaacg atcaatgcca aatctttggg
    49021 ataagttaaa gtcggaataa ttgccaacgc cagccagact ccaacgactt catcaattac
    49081 tatatacgct gggtccatct cgccaatttg tttttcgata tactcagaag taaaaatgcc
    49141 aataaaaaaa gtgactgtca ctagaattaa gtcgaaccag aatgagtgcc atggcaataa
    49201 gaaccagaca actaagcctg ctactgtacc ccaagtgccc ggtgcaaatg gaagatagcc
    49261 gatgaagcaa acgctcgcta ttatccggga gagtagtttt ttagctccgg taatattcat
    49321 tttttcagag cttgctgaat tttatcttta tttactaaaa tatattccac cccagtccag
    49381 attgtaataa ttgtggttat cagcatcaag atataaataa ttctatattc ttcaattaac
    49441 ctgacatagt cggagatacc cagaaatatt tcccagcgct gagtaatcaa ataagctaga
    49501 atgaaacaga caacgccgac ttgcaccccg gttttcgttt tcgctgattt gcgagtttcc
    49561 attgtcaggt ggcgcgaatt cattaatatt ctaataccgg ttacgataaa atcacgtaaa
    49621 attaccagaa caaccatcca gagaggaacc agatctaaaa taacaaatga aataaaggcg
    49681 gcagacatca gaattttatc cgctaaagga tcaagaaatt tgccgagctt ggtcacgctc
    49741 gcatatttcc gagcaatata gccatcgtag atgtcggtta ccgaagctat gataaaaatt
    49801 aacaaggcga atatttcaaa ataaggttgg ttaccgaaca gaaaatagag aaagagaggc
    49861 gttaggagaa tccggaaaaa agtcaataga ttaggccagt taaatagttt tttcatatta
    49921 gaaacttgtc ctaccctcaa gagcgtgcgt cagggtaatt tcgtcggcat actccagttc
    49981 tatgccggcc gggagtccct gagcaatacg agaaaccttt acatttttac ttttaagtaa
    50041 tttggaaaga tagagcgccg tcgtctcacc gtcgcgggta aggttaatgg caagaattac
    50101 ttccttcaca ttggcaagtc ggtataacaa accttgaata ttcaggtcat caggcccaat
    50161 cccgttcaac ggcgacaata ctcctcctaa aacatgatag aggccgcgat aatgaccggt
    50221 cttttcgatc gagaacacat ccatcggctc ctggacaaca cagatcgtcg tgtggtcgcg
    50281 tttttcatcg gtgcaaattt tacaaggatc ggtttcggaa atattataac actgcgaaca
    50341 tttataaatg cgatctttga catctataat ggctttggct aatgcaacag cttcctcccg
    50401 atcggctttc agcaaataga aagccagacg ctgagccgac ttgcggccta ttccgggaaa
    50461 tcgggcaaaa cgttcaatca aattttgtaa cgaatcggga agagcactca taaaccgggt
    50521 attttcattc ctcctggtag attggataac attccaccgg ttaagcctgc cattttttct
    50581 tgcgataatt cctgggcacg agtcaaagcc tgattgacag ccgcaacgat catatcttca
    50641 accatttcta catcgtcttt taatatttcc ggatcaattt taatagattg aatttcctgt
    50701 ttaccattag ctacgacctt taccattcca cctccggcac tgccttcgac agttagatta
    50761 gcaagttctt cctgtactcg ttccatttcg gcttgaactt ttgaaaattg tttcatcaga
    50821 ttatttagtc cacctttagg tatcatcaat actccttatg taataattat ttcgccatca
    50881 aacatatcta cgactttttc agtcatttca tcgatcggct tttttacggt atcagccgat
    50941 tccgcagctg attctatttg gatacaacga attttgatcg gtttatggaa aaaaccaatc
    51001 aaagtctgtt cgatagtcgg tgctcttttg gcaacatttt tacgagcaaa atcctgttca
    51061 ctggtaaaac ctagatctaa aacatttcca tttaattgca ttggctttga aactttaaga
    51121 aattcagcca acgaaagatt gctttcttcc aatttcaaaa taattgacga ccattgtgac
    51181 tgaatcgctt caaacgtgac taaactatca aacgactttg gaattggttc agattttttt
    51241 tcaggtatat agctatcaga tactttctct ttaataacgt tactatctgg atgaggggct
    51301 gttttggaaa ccaccggctt ggttgaaaaa aaatccatcg tttgggcgac tggctttatc
    51361 tgctccattt ccccactatg ccgagtatca atattctgca ggatcgattg aatcgtgaca
    51421 gtcttttcca ttgctcccat tcggaggagg gcaaattcga gatgagttcg ttgattcata
    51481 gccgctttca gaccagtctg cgcttcaccg actaatttta ataaacgcat cagatcatta
    51541 gtggaccatc gctgagcttc ttgctgataa tgatttttca atgaatccgg aagatccaga
    51601 tcggcggcgg atttaacaat cgctgtaatc aacaaattcc gaaaatgctc ggatagacca
    51661 atcagaaact cactgatatt tatgccattg ttgaatactt cttcagctga cttaatcaaa
    51721 gcattaagat cgtgctcgaa aacctgatta ctgattgtaa aataatattc ataatctgtc
    51781 agaccaagca atttttggac agaattcaaa ttcacattgt cactagagaa cgaaacaacc
    51841 tgctcgagga gactttcggc atcgcgcatc gctccttcgg acttcttggc taacaaagtc
    51901 aagatatcat ccgaaaccgt taaattttca gattccgcaa ttttctttaa ttgccccaca
    51961 atttcacgaa ctggaatgcg atgaaaatca tacctttgac agcgggaaat aatcgtaggt
    52021 ggtactttca atggttccgt agtagcaaag ataaaaacaa cgtgcggcgg tggttcctcg
    52081 agtgttttca aaagcgcgtt aaatgcttcg cgcgtgagca tatggacttc atcaatgatg
    52141 aaaattcggt atttcgaatt ggatggcgga tacttgacat tttcccgcag ttcgcgtacc
    52201 tggtcaattc cccgattgga agcgccgtca atttcaatca cgtccagact gcgactattg
    52261 gttatttcga cacaattctg gcagacatta cagggattat agtcttgcgg attaaggcaa
    52321 ttgagtgcct tggctagaat tcgggcagtc gaagttttac caattccgcg cgaaccggta
    52381 aagagatagg catgagcaat gcggccactt ttgagggcat tttgcagagt cgtacaaatg
    52441 tggtcttggc caatgatttc attgaaattt tgagggcgat atttacgaga aattacttta
    52501 taattcatat ttacttaggc tatgcaccag gcattgaatc cgtccaggga tattcggtta
    52561 aagtcgtcgg ctggctttcg ctgattttgc ggcacataga aagggttatc taccgctgct
    52621 tccttccgga cctgacgggg tttgataatt ttccattgcg caaaactgaa agcgtcatcg
    52681 cttccgattt aacctattat ccccgaccaa atcctctgcc tggcattcca ccctgctaaa
    52741 gcggatttca ggttacaggg caccgctagc tccccgtgta gcatagccaa tcattttgtt
    52801 cgtatcaaat aacagtggag ctgatgggga tcgaacccac gacctcagag atgcgaaccc
    52861 tgcgctctcc cagctgagct acagccccgt gttcaacgta tgataatttt tagtggtcac
    52921 tttttcaggt acgattgaat ctcttccccg ctctagttga aatcagttag atagactata
    52981 attccgttat taccccgcga ttcagaaact atgtcctgac caccgttaaa atcgtattct
    53041 attatcaaat aacagatatt tttaaattac taaaaatcag ttaatctatc aaaagataat
    53101 tgaatactta ttttctacga ttaaaaaacc tcctcttcgg ttcactttcg gtttttttat
    53161 tcaattctga ttctatgact atgccagctt ttctttgttc actttcctct atccgttttg
    53221 cgcgttcacc tgatttagat tcaaccgggg tgctaacgct ttcagaaaca ggagtccgtt
    53281 ctggcaattg attgcgttct atttccagct tttcaagctt atctaaaagc gcaataacct
    53341 ccgtatggaa atcgagaatt gttttttcca aacggtgaag caattccttg gtcaggcgtt
    53401 cgctataaca ctgaccaata ttttccataa tgtcatctaa tttactccga atatattcgt
    53461 agcgttgttc ggtggcgcta aagtcaattt tgacttcgag cgccggaggc gtttcttcag
    53521 ttggagaagt cgttcgccgc gtggaaagat ttttgtttaa ggtgtaataa tttgccatat
    53581 tttatcctta atatttctaa tattgtaaca cattcttgcg atagtgtcaa gtctttttat
    53641 attacccgtc ggaaatttta tcacagtgga cggcataata tattcattga ctattggaga
    53701 aattttatat ccgataacac aaccgacgga ggcggccgcg aactgagtta aaatcgcatt
    53761 ttttgtggta atattaagcc aatcggctac cagcccacta ccaaaatatg atataaccgc
    53821 gacacttccc atagtacccc acttgaccca gttattcgga cgataattat cccagtattt
    53881 taatttgacc aaatcgttcc tatttattgg aacaacctgg cgatcaatcc aaaataacaa
    53941 gctatcaccc ttgagacgat aataaaccgc atcaaattga cttttatctt tcaattttag
    54001 acgacagtag gaattcatcg gtatctcagc taaaaatttt tcggcgtata aaggcttgta
    54061 tctcttttga atcaattgac ggttctcgtc ggtgagcggc tcccggacag agatacgtga
    54121 agcgattagg tacatctgat aaggagtaaa atataacaac tgctcttgat atccattgtc
    54181 aatccataac ctgattttag ctattacagt atcggaacta gtttgataga actgggcatc
    54241 ggaaaagtta taaaactctg gaaagaggtc gtaataaatt ttctcttcta aatcaatcgt
    54301 ggcgcctaca ttcggatgaa ttacgagaat ctcctggcta ttcccagctc tgaaattgat
    54361 aaagagtgaa attaacaagc tcatgataaa cgctaaatta ccccgtccgg gaagccccca
    54421 cattaaatga gggctttccg gtttggcgct ctcacttttc aatttataaa acgccttaaa
    54481 aactaaaccc agtttcgatg gtagttagat taactgcttc cagatcgggg tcaactattc
    54541 cattaccgtc aaaatcacgg taagttctct ggaatttata gtagataatc gctccgccac
    54601 ccaattcaaa acctatcctg taaccgagta tcgtattttc agaagggttc ttaaaatcga
    54661 aaggattttc atcattatta cgttgataaa aagctgtcgc atcagccagt tttggaacta
    54721 ttcccttcgg tatagatgcc gacgccataa aactcttgat ctcggtccct tccgagaaca
    54781 tgtgttgata ataaccacca agaatgaaat aattcatgat attgacgtca atagctccgt
    54841 aaattccctg catcgaattc tgcatcagag acatttgttg tttggtgtat atttgattat
    54901 tacgaatttg tactcgctca taatcataag ctcggtccca gtaattgaat aggaaatttt
    54961 cagcggcata acggtattcc agagtcagat ttgcgattct ggcgattccc attttcacac
    55021 ctgggactgc aaatccatat cccggagtaa aagctacacg gtcataactc tgagttgcac
    55081 tgttataaac tacggctgaa tcttcaacgg ggaaaaagga ggcggcttgg ccataaaccg
    55141 tcaaattgaa ggatttctta ttcagaatag gcactcctac atcaaccgaa accgctccat
    55201 ttaaaagccg atcataatcg gagagcttag ttatcccatt ctgtatgatg gtttcacttg
    55261 gaatattaaa gccgttattt ctaagaaatt cgcgttgacc taaagtaaaa ctggttttaa
    55321 gtgaatcgag gacaacattg tcgtcagcct ccggatataa atccagcgcg tcggcgacat
    55381 catcttcatc cgcatccggt aaacctttgt aaggataaac atcggcaaca acagtagttc
    55441 ccagaaccaa cggaaacttg ctaatcggtc tataagtcaa cctcccagcc attaatcccg
    55501 gtccggtaat ctcttttaaa ttagccatat aggcttccca tccgatttta ctgagttgca
    55561 ttccggtatg aacaccgact ttgcggactt gcggatattc cgtcgtattg gaatagccgt
    55621 ttaataggat tccatacccc atactaacat tatccaacgc cccaactttg atgaagaacg
    55681 gatccccctg ctgagcccaa cgcacataat atattttgtc aaaataatcg gtaaattcat
    55741 cccattcttc tttgcggatg ttgccatttt cgtccatata aaaggcaata tcaagggaaa
    55801 cgcctaattt cccaaattta aattcgggac gcagggcaat ctgattataa attttcccgc
    55861 caatcgtaac tgagccaagt cctaatccca tcccaaaggg ttttgctttt tgggcttcct
    55921 cctctttttt acctgcttcg gctactgatt cggccggtgg ttgaccagtc gcctcgatgg
    55981 cacccattgc aggcatttca gtttcttctt ccatcttcat tcccgatggt ttacccggct
    56041 cttcttcggg tttcatctct tcaggatcag cagggatctc atctggatct accggtaaac
    56101 tgaatagttc tccatcaata gtcgatacta ccatttttcc cggttccaag tcagcctcca
    56161 aacccgtaat taaatttaaa atacctactt gcccttcgac accataaaat ttatctccga
    56221 aatcgggaat ggtaattgtc cagaatttgg ttcccttaac cgacgccacc gaagtcggag
    56281 tctcaatccg gaaggatgtc ttaatgcccg gagtaatttc agccagaact ttaccttgag
    56341 taacttttga ggttcgatcg cctaaattat ccttaatgag ttcaatttcg gaatcattgc
    56401 gaattttaag cagactttta tcctcggtga agataatagc tacaaatcca tcggaaccag
    56461 tctttagcgc atctccggta ttaatgctca tacccatggt cacctttctc gaaaaatcac
    56521 tacgcccttt agtccgtaat ttgacgtctc cggctacttt cagaacagtc gccatctgat
    56581 tttgagcgat cgcaagattg cataacagta aaaatgctaa cgaaacgata tacttcttca
    56641 ttttttctac tcccatttaa atttttatct atctgttatc atactaacaa ttcccattac
    56701 gactttagag tcccgtaatt caagtatggt aaaatccatt ttagctgtag tgtcattgtc
    56761 gtttgtattt tttaaacgta ttccaaatga tttgatcaaa ccataatcaa cctgctgaat
    56821 caattcatta aaaggcttat ttatatttgg attaataaaa tcgttaatat ttttaccgat
    56881 gatctccggt cgggattttt gtacccaatt cataaagcct cgcgagatgc cgattattgt
    56941 gccatctttg agaaataaaa tctgattttc gacagcagaa tctatttctt gataaagacg
    57001 atcatggctc tcgcgaatct ctttatacat atcaactaat ttttcattaa aaacttgtaa
    57061 cccgctatat ttttcattca gagtttcaac ctcttccgct aatttacttt taatattttt
    57121 gataatcaaa tcagggtaat tttcaatgaa atcttttaat ttgtatccta atccttcgat
    57181 agcctcaatt agcagtatat tttcatattt atcaattgat tgtaatttat aaccatcttt
    57241 gaaataatct gaaccgtgtt cctgcaacca atcattaaaa ttcttcttta actttcttac
    57301 attaaattta ggcccgatta aggcgacaat ttcagattct tccggacatt taccgaggat
    57361 ttttaaactt tgttgttcat cggcttccca taaatcaaat tgaaaagtat tggtaagaat
    57421 cgccgaaacg tcacctttta gcaattgatc taataatgtt tttggctcat agtatattga
    57481 taatgatttt ggagcaaaaa atattccgca atttttaatc aagtatagaa tcaccggatt
    57541 gacgcaatta tgatctattc ccttagtaat cagcacctta ccacctgcca gttctttaag
    57601 atgacgaaca gtcgctttct ggggaataac cagaatatta tcagatgatt ttgttttaat
    57661 tttaaagagc ggctgaaaat tttcaaattt ctctaaataa atagaatcat agatagaaac
    57721 aaatacgagg gcatttttga tgctggttat ttgttcataa ataggcgggc tgttttggag
    57781 gttttgaata acgatattca agttcagttg attatgtaat atgcctcgga tagtattaaa
    57841 gatactgtta gctttgttag atgtcattcg ttcaggtaaa aataaaatgg cagtatccat
    57901 tgttattacc ttattttttt gcgagattgc ttgaaataca tttctcgaga acatccagat
    57961 caattggttt gggaataaaa tcaacgggcc ctacattgat gatatcgtca actacctgtt
    58021 tatccttcac cccagttaag ataataactg gtaaatcggg atataatttt cgaatacgtt
    58081 taagtacttc aaggccgctc attccaggca tcagaatatc taataaaacc agatccggat
    58141 tatttttccc caggtaacgg agcgccttgc tacccgatct ggctttgata actaaatagc
    58201 ctttatcctg caaaaactcc ccgagaaatt cacaaacatt ttgttcgtca tccacaatta
    58261 atattttata ctgattcatt atttcccctc taaatttaaa atctttattg atagctaaac
    58321 atttttcctc ttaattctga gacacggatt aatttgggtg atcagtttca tatatttatt
    58381 tcttaattat aatatattca agaacaaata gataatcaaa taaatcttaa tttattattt
    58441 tcaaatattc ttgaatgtcg gcttctgaaa gaaacatcac atctatacgg ctgaggtttt
    58501 tcaattgttc gaaacttatg tcaaagttgt attcggcttt cgcatcggta tttttaagtt
    58561 ggatggcgac attccaaccg ttagccgtga cattgattaa aggctgatat ttggctgtaa
    58621 attgtacttt tgaagaagaa ataagatttt tgcggtacat tattggcata tcaatcagaa
    58681 cgcaagctac ggtttcggtg ttagttataa atgcatatac cgggtgagtc cgataatcgc
    58741 cacgcgcaat cgccttaata aaatcttcgc tataggcaaa ccgcttacca ctgaaaatat
    58801 attcggtata ttcatcattt tttgtctgaa tttcataagg aaaagttgtc aacatatctt
    58861 gaattagatc acgactaaga acataattaa taaaaaaagt aacgttaatt atctcatcgt
    58921 ttgcagaaag gcgccggaaa agtggatcgc tgatttcaag tttataggga ttgcggatga
    58981 tctgattgat agtatattga aaactaccag tttgggcaat cgcctctccg aatttaagcg
    59041 aaagttcatt agtcacggtg ccaccaaaac ttggttcgtg ttgcgctgac tcaggatgag
    59101 tttccgaaaa cttatcaatc actgatgaga ttgctacact tgcggcaaca gccttctggt
    59161 aattattttt cgtcggttga gatggtgagc taatttcctg agcgacggtg gtctcggctt
    59221 tttttattaa atatggagta agtacttcag tcgcgccgtg attgacagat tcgataatct
    59281 ttgccatatc tgctgattga atcgagatgt tctttttctg gagaagtcgc caggtattaa
    59341 tgtcaattaa ttccaaatca acctcgaatt tttgatcaat tattttataa ttgccggtta
    59401 aaaggtagca tctggtaatt tccgtttctg agagattctt aatttggttt ggtatggttg
    59461 ccgggtcatt gatgcgatgg acattaagat ctggattatt actgaaatag tccatcaaga
    59521 aatcaacaaa gccttctttc caccaatcaa tttctggcgc gccctcataa ttagtgaagg
    59581 tgagaatgaa aagattaatc ggatgagaat tgggcgtctg cgcaccgacc aatccaaata
    59641 ataaaaatga taacattaga aaatatgcga tttgccaacc atttttcatt ttaattcctc
    59701 aggttttgaa atattcaatg gctttttgta ttcggcgcag aacggtttct ttccccagaa
    59761 tctctgctac ttgaaaaata ccgggactaa taccgaaacc ggttaaagcc agacgcagag
    59821 gatgaatcaa atcagcggct tttaatccgg catgttctgc ggttcgtctc aatgtttctt
    59881 cgatagtatt agccgaaaaa tctgtcagcg ttgccaactc ttgacttaat ttagccagat
    59941 gttcattggt ctccggtgtg tgccagtatt tagcggcagc tttttcatca taattttctg
    60001 ggtcttggaa aaaatagcag ccccattcga ggtactgatt ataagttctg acgcgaccgc
    60061 gtaataactc gataacggaa tcaacgtact gagtccggtt ttcagattct tgctgattga
    60121 taagcccggc cgcaattaat ttgggaacta aaaccgatct aattttcgcg acgggcatac
    60181 ggttaagatg ttcaccgcta atccattcca atttctttat atcaaaaaca gccggtttgc
    60241 gggaaattgc ggtcaattga aattgttgga tgatttcgtc aatactcagc acttcctgat
    60301 cattctgcgg tgcccagccc agtaaagcta aataattcac gacggcttcg gcgggaaatc
    60361 cctgttcgcg atattcgtcc acgccggttg cgccatgcct tttactgagg cgcttattat
    60421 ctggaccaag cagaagcggc aagtgagcaa attgcggaat cggctgacca aacgcacgaa
    60481 acagattaat ctgtttcggc gtattcggaa tatggtcttc accccgaatt atatgagtta
    60541 ttcgcatatc aatgtcatca acgacaaccg ccatctgata gactggaact ccattcgaac
    60601 gttgaatgat aaaatcatcg agttcattat tattcaccgc aatatgtccg taaaccagat
    60661 catcccattc aatgactccc ggttcgagtt tgatacggac agtgtaaggt ttgttctgtg
    60721 ccatcagttc ttctgactgt tggagcgata gactacggca tttaccacta taacgataag
    60781 ctttattctg tttaattgta gcggcttttt ccgcttccag ttcctcacta ctgcagaaac
    60841 agcgataagc cttgccggct ttcaacaatt cagtaacttt ctgttggtaa agttcgagtc
    60901 tctgcgactg ggatactggt tcttcatccc aattcaatcc cagccattgt aacgaatcgc
    60961 aaatttgatt caccaattct ttgcgtgaac gctcgacgtc ggtatcttca atgcgcaaga
    61021 gaaatttgcc accgaccgag cgagcaaata gccagttgta aatagctgtt cgcgcacctc
    61081 ccagatgtaa tgaaccagtt ggactaggag caaaacgaac gcgggtattt tcagtcatat
    61141 attttctaac atcatttatt ttcaatcaaa cttattttaa attcagcata aagttctcta
    61201 aaaaattata cttaatcaag agttttggct cattgatgta tttcagagcc cattttttca
    61261 aggaaaagtt aatggttagg gttgacggag tacagttaat aataactttc tcaatgaaaa
    61321 ttaataaaat acaagaattt taaaatattg atttttgacc agcatttgaa taaaatacac
    61381 aaccaaatgg atatttagaa ttttcatgca aaatatttca aaatatagaa tccttcaaaa
    61441 tattttgctc ttattttctc tcgcatcttt tgttttcgct gatgaagtct ggctattaat
    61501 caatcccgag acgcgttacg catcgttagc cggagccgcc acggcgatcg tcaacgatgt
    61561 cgcggcactt tactataatc cagctggttt agccaattgc gatgaattaa gtgcctcagc
    61621 caattatggt acctggctcg gcaacagtga aatatattat gccggagctg ttagaaattt
    61681 taaacaccgt ggcggtttgg gaaccagttt atacctgtac aatctcgatg cgaaaataac
    61741 cggagacgat tacactgcaa ccgatttttc ctataacttt atctatgttt ccatcggata
    61801 tggatgcaat tttaccagaa aaattactct aggagcggca acaaaattca tatatactaa
    61861 cgccccgaac actgcgagcg atggcattca atctattaac ggcaagggtt tagcctgcga
    61921 cctgggtatt cagatcaaca atctcctgcc attcctaacc attggaaaaa ataaaacagg
    61981 tggattatcc ctgggcgccg cattctgcaa tctcggaccg gctattaaat accgcaatga
    62041 ttttaccaat tataagtcga aactcgtgcc tcgcttacga ctcggcggtg gttggcgact
    62101 gcttaataag gacttgatca gcgctacgat ttcctacgat ttcattgaag attattatga
    62161 ttctaactct tcaaatcatt taaaaagtca cttaatcggc ggcgctttca gcttctacaa
    62221 gcgactttac atccactgcg gtcaacatat tgaaaacaat gaaaattaca taacctgggg
    62281 cgtggcatac aacctagata aatttatcat tggctactgc cgttatccat cgcaaaaaag
    62341 taacttcttc agcgtatctt attccttata aaataagttg ttaaaattat tggcaggatt
    62401 gaactaaatc aatccggatt aaaaacattt taccaaaaag aaataaacta taatccgacc
    62461 ggtaggacta ttcggtcgta gattgttctt gcggtagatg ccggaagaaa atgaacagac
    62521 tgcccgccaa aatcaggagg tcttccgcca ctcgcttcca gctgaccagc gaaccggttt
    62581 tcgatagagt aaagcaaccg cattcgatat tcagaccacg tataatagcg ctgataatca
    62641 agcttataaa tacaatgatc aaaatactta gtagaaaagc cgctgtttcc agccagattc
    62701 ccaatattaa ggttacacca agcgcaatct ccagccatgg taccaggata gccgtgatgt
    62761 taaccaattc aacaggtaga acgcgatagt tatacacaat cacggcaaac ttttcggggt
    62821 ggatgatctt gccgacagcg gcataaatga acaagaaacc aataaccaat cggataaata
    62881 attccaacca atcccagaat ttggacttca tggcttgctc ccttttacaa tcggtaaatc
    62941 agcctctacc cactctttcc agccaccgaa ataaaaccaa acctgctcga aacccatttc
    63001 atttaagcgt actgccatat caatacttga ctgacaatca ctgccgtcac aataggtcac
    63061 gatccgcacc tgccggtcca attcctcaac aatggttgga tcgttttgaa attccgacaa
    63121 tggaatcgat attgctccgg caatatgccc ggcaagatag tcctcatatt cgcgagtatc
    63181 gataaagatg gtaccgccat tagtaaaaaa ttgccaggct ttttcggtat caagctcgga
    63241 aaccgggctg atttctagat cagtttcctg attagtctga accttgcgat tcataaaaag
    63301 cggaattttt tgactcttac ctcggactgt aaccgtctga tatttagcca caagcggaat
    63361 accgttcggt gataaccaat tgaaaattaa cccgactaca ataacgccca gaccgagcca
    63421 gatactacgt aaaagaatag ttttaaccat ttgtttcatc gtgatactta ataccatatt
    63481 taaaatattc taaaaaattt gtttttcaaa acaaagaata taaattttta ccgttataat
    63541 gtcgagtaaa aattcaatac cagttatcaa atttctaagt tttaccctga tcttatgtct
    63601 aggcgggctt tcatgcagtc ctcagcgctt tctattcaat aaagtttcca acgtcttcga
    63661 cagcggtatc gaaataatat atcaagaagg cgatttagaa ctcgctgaaa gattcctttc
    63721 gagtaatttg gagacaatcg tcattttagt tgcgcaggat ccagagaatc cgcgtttaaa
    63781 cctgatggcg gctcaggctt ttggagccta tgcaatggct tttgtcgaag acaaagaccc
    63841 tcagcgcgcc ggtctgcttt atgttcgcgg cttagaatat gctttcaaat cactaccacc
    63901 gcaaataaga ttctcgcgca atatcacgcc tgccgacctg gaaaagctcc tggtagaaag
    63961 ccgtaaaagc gacgtgccgc aattgttctg ggtcggatat aattggggaa tgtatattct
    64021 gcaaaatctc gatcagccgc gcttattggg ggacttagct aaggttgaac tcttgatgcg
    64081 tcgagtagcc gagttggacg ctggctataa tttcggcggt gtggatatat tttacggaac
    64141 attttattcc gctcgcccac caatgctggg tggcaatccg gaattggggc gcagttatta
    64201 tctgaaaaat atcgaacgca atccgaattt tttaatgacg aaattgtatt acgctcgcta
    64261 ttacgctgta caggttcagg ataaagaact atttgataac cttttaaccg agatcgagac
    64321 tttcgacctc gagagcgctc ctgaaattcg actctttaat gcgcttgcca aacaaaaagc
    64381 caccaatctg cgccagaatg ccgagttgtt tctcgaccta aatgaaatat tagaaaccaa
    64441 cgaaaggtag attaaaaatg cgaatgaaat atatcaatgc ggtgattctt ataattttat
    64501 gtacaattcc taaccttgta gctcaaaaag tccatacgat aaaagttgcg accgtcgcgc
    64561 cggaaggttc gagctggatg cgacaattcc gacagtttga gaaggaagtg gatgctctaa
    64621 ctaacggtca ggtcaaattt cgggtttacg ctagtggtgt acagggcggg gaaaaagaca
    64681 ttttacgtaa aatgcaatta gggcaattgg atgtcgggac tttcaccgga gtcggtttgg
    64741 gtgaaattgt accggaagtg cgcgttttcg atttgccgtt tctttttcgg aatagcgccg
    64801 aggttgatta cgtttgtgac gctcttttcg acgaatttca aactaaattt ctcgagaagg
    64861 gatttttttt ggcaggttgg gctgaagtag gttttgttta tcttttcacc aacacgccag
    64921 tcaccagatt agcggatttt aaaaatgtca aaatgtggct ctggaaaggc gatccgcttg
    64981 ccaaagccac ttttgacgaa atgaaaatag cggcgattcc actcgatatc accgatgttc
    65041 atactggctt acaaaccggg ttaattgacg gagtttatat atctccttac ggcgcgttgg
    65101 caatgcaatg gcataccaaa actaaatata tgctgaatta tcccctaact aattctattg
    65161 gcgcaatcct gattacgact aaaaaaatga acactattcc ggaagattta cgaaatattc
    65221 taatcaaaaa aaccaaggaa aatacacgcc agatcgtgct ggctggtagg caggagaatt
    65281 tagagtctat ccagattctt aaggaaaatg gtatgcaact cacaaacctg aaaagcgacg
    65341 cggccgccga attcatcgag gccggagtac gaacgagaga ccgattggct ggtgttttgt
    65401 attctcgtga aatgctcgac cgggttctgg cgcttttgga tgaatatcgc gctgagcatg
    65461 ctgaataaaa ttagaactat aatcgaaaaa tttgtcaccg ccttagcggt tgtcttgatg
    65521 ctatttttag ttgtaatggc tttcctgcag gtaattttgc gcaatttttt tgcgattggg
    65581 ttaaatgttg tggatgagtt gatgcgcaac ggagttttgt ggatcgcctt ctgcggggcg
    65641 gcattaacga ccttacgtag taaacacatt tcgattgaca ttctgccgcg tattgtaaaa
    65701 ggcaaagctc gcttagtgtt aaactggatc ctcaatctaa cagcggcggc gatttgcctg
    65761 tgtctcagct ggtatgcctg gcaattcttg cgtttagaaa taagtatgaa ttcgatgatt
    65821 gccggagtta ttccagcctg gattatcgaa attattctgc cggttggatt tttcctgctg
    65881 gcattgactt ttcttctgcg tttagtggat aaatctcaaa atctcgaaaa ttaatggctc
    65941 tgttgttttt agttctgata gtcgttttga ttttttgcgg attgccgctc ttcgcggcaa
    66001 ttggcataac cggcattatc caattcgcca tttcgggaat tgatccaacg gttgtcatcg
    66061 tcgaaatgta ccgggtagct tccgcgccga ctctcctggc aattccctta ttcacgttag
    66121 ccggttacgt tttagctgaa agcagtgcac cgcgaaggct tttgaactta gcggaggctt
    66181 tgttaggttg gatacccggc ggcgttgcaa ttggctcact tgtaacttgt gctcttttta
    66241 cctgcttcac cggtgcgtcc gggattacta tcatcgcctt aggcggtttg attctaccaa
    66301 ttttgcttaa aaataactat tcagagaaat ttgctctggg actgattacc agttccggct
    66361 cgctcggatt attatttccc cccagtttgc ctttgatttt atatggtgtt gtagcttcgg
    66421 tttcgattga tcagctattt attgcggggc ttattcctgg aattctatta attatcctgc
    66481 tctctattta tgctgtccgt aatgatgttt taaaaaagag cgaacgaata cctttttcct
    66541 ggcagaattt agggcgagcc ctcaaagaaa cggtctggga attgccgata ccggtcatta
    66601 tcttgggtgg aatttacggc gggtttataa cggcggtgga agcttcggct gtaactgctt
    66661 tttatgtatt gatagcggaa ttcatcctga accgtgattt atccttccgg cgggatttga
    66721 tccgcattat gcgcgatagt gtgcaattgg taggcgggat tttgattatt ttaagttgtt
    66781 cgatgggagc gacggcttat ctaattgacg ccgagattcc ccagaaagct ctgctctggg
    66841 ctcagcaggt tttttcttct aaaataacct ttttgttagt attgaatatt tttctattaa
    66901 tcactgggat gatgctcgat attttctccg ccattattat tgtcgtgccg ctgattgttc
    66961 cgattgctct cagttatggc attaacccgg tacatctcgg aattattttt ctcactaatc
    67021 ttgaaattgg ttacttgact ccgccaattg gattgaattt gttcatttcc agctatcggt
    67081 ttgataaatc cataacggaa ctttatcgga tttctctgcc gtttctattt attatgctaa
    67141 ttgctttagt attgattact tatattccgg atttgagttt gtttttggtc ggattgatac
    67201 aataaataat tcgtagaaca gcctttctat gctgtcagtt cagctttatg ttgtttaaaa
    67261 aatagacagc cagtaataat tcatcttggg caatttacta aatcgcccag tcgaaatccc
    67321 atttcgacct acatcttcat atttataggc aggaatgtgt tccctgcgat tgattttatc
    67381 aatctaaatg gtacgaaatt gaaaaaaatg cattgcgggt agcaccaggc caataataag
    67441 catagtattc accccattca tagccatagc cataagtcga gtaaagccgg ttgaataagt
    67501 tattgacttt cgcgccgatt tcaacccgac cgaatgaata tctcagtccg acatcagtta
    67561 gccaataagc cggattcatt ccgatattgg cttcgtcaat atattgttcg ccaacataac
    67621 ggataatcgc cgaaatcgac aaatttttca ccggcttgac atccaaactc ccattaaaaa
    67681 gtatacccgg gatattgggt agagtattac ctttatagga accactttca aaaacatggc
    67741 ggctgaaagt gccgtttaga gtcaatgccc agagtgggtt gaattgatag cgaacttccc
    67801 attctaatcc ttcatgaatg gtcgccgacg cctgtgtata cacatattca ccttcctgct
    67861 cgatatcaat attttttaac tgttcatttt tataatttat ccgataaaga ttcaggtcaa
    67921 catccagctt tcgacctcga tagttggcac ccagttcgta atcatcaatc aactccgcgg
    67981 cggctttttg aggttcgctc caataatcgt cggcttcaat aatttgttca tcggctggtt
    68041 ctttttgcga gcgtccgtaa ttagcaaaaa ttgaaaagtc atttgatagt tcatatatag
    68101 ctccaaatcg aggattcagg aaatcccact tagcggaaat atcataccca atcgcataac
    68161 cgattttttt ctgatccaga ttccattgca agtgttgata ttgcaaatca gtgatcaatt
    68221 tcaatttctt tagcggttca agcgtaaagt ggacaaagcc ggttgcgatt gacttttcac
    68281 cgatataacg ataatagcga taccagcctt tcccaatttg cgcgatcaaa ttctgatcgg
    68341 aaaatcggga aatttcacca aaatgattgc cgttatagta tttaaattcc ccgccgatat
    68401 cgaggcgata cttagctttg ataaatgtca aagtcggtac gattccgtaa tacgaattca
    68461 caatccattt acggcgggtt aattccagtg actggctgtc agcatcagtc gggtaatatt
    68521 gatcaaggta gaaagaacgc aaaaaatccc gaaattcatc tcgggcagtt gcatcatcaa
    68581 cagccagatt ataggcggct gatttttctg actcgtaata accgtcacct ttcaccagat
    68641 aagcaacatt attgaagtta agattctcgc taatccgcca ggtgctattc aaggaataaa
    68701 cctgctgaag aaaattatct atatacgatt tgtaaccgga acgacgtccg actcgatcat
    68761 tgatcaactc ggctggaacg ccatcccaca tcaagttact tttttggtaa ccaaccagcg
    68821 cccgaaattg atgagaagta ttttgtccgt tatattctat accggcagaa ccggaacgct
    68881 gaaaggtttc atggtattca cgatagccgt ctgattctat ctgcgagaga cgggctgaaa
    68941 ggcgcagctt tccattgtgt aactgcccgg aggaagcacc cagacgtatt ttcgaagtat
    69001 tatacgaacc gccgcctaaa tcaattgaaa acgtcggtcg gtcggtccca atcgtggtca
    69061 agacattgat cgaaccaccg aaagccgacg atccgtataa gctattgccg ataccgcgtt
    69121 gaatctggac atcagccgcg tcagaaagaa tatcaccgtg atcaacccaa taaacctgat
    69181 gactttcatt atcattcaga ggaacgttat ttaacatcac cgcaatccga ctctggtcaa
    69241 agccacgaat ctggacgtag gaatagccgg taccattgcc gctctcacta taggaataaa
    69301 caccgggttc tatagccaaa atcatcggta catcttctac ggtgtagcga cttttgattt
    69361 cctcaatgga taattccgaa aaagccaccg gtgtcttgcc tttgactgcc cgcgtggcag
    69421 taacgaaaac atactcgccg gtaattacgg ttggctttaa gtaaatcgta ccgcaatctt
    69481 cggaagtagc tgaaaaagac gtatcccgga aagcaatgtg agtaacagtc acttttatcg
    69541 gaagaaacga tgttttgatg cgaaaattgc cgtccttgtc cgaaaatgta ccggagccat
    69601 cttccagcaa aatattagcc gattcgactg gctgaccagt tgtttcggat actaattttc
    69661 caaaaattaa atgttcttca ccgaaagcgt ttgcctcggt taaaataaga atgaaaagaa
    69721 tgaagaaaaa tacaggtagg tttttttgat gcataattac tcccttcgct ggtattatcc
    69781 agatcaggtt caaagggtaa attctcagcc gccccgtcaa acgggttagc acccccggac
    69841 catcacttgt taaaaatcac tggaaattaa taactcgaaa aatcagacgc aactaatttt
    69901 tgactttgcg gaaatgcctg ggagtcgaac ccagcttccc gattagatcg ggaacgacgg
    69961 ttttgaagac cgcggccccc accggggtag cattcattcc catattcatt gtttgacaac
    70021 aataaaatct aagttatgat cttttttcaa tttatttcca accgcgcgcg cctcctcttc
    70081 cgacttgaat gaaccagcca gaaccgccgt caacttgcgg ccggatcttt ctatttggcg
    70141 caaagtaacc gaatacccca gtttctctaa tttacttcga agcgccctag cgtttgctgg
    70201 atcaccaaaa acacccattt gcaaagtaaa gttgccttgt cctggcactt ctacttttgg
    70261 cagagaatag gtttgagtct ccgccgattt agcgttagaa gatatttgac tgctggtcgg
    70321 ttgagattgt ttcttagatt cgatactagt aaccttagaa gcgattttgt aatcctcaaa
    70381 atgaaaattt aggttagggt actgagtcga ataatactta taataataat caactgaatc
    70441 ccgtttatta agggcgttaa aactgcataa tcgaagataa attgctttgt caatcaaatc
    70501 cgattgtgga taggttttga tcaattcact ggcgtatttt tccgttttag tatacaagcc
    70561 tttagtaaat aaatattcga taattttcag taaagcatcg tcagcaaccg agctgctggg
    70621 gaaacgactg agcacatcac gataaattag caaagccgct tcaccatcag tttcaatcac
    70681 tccattaata taaagcaact cgggattatc gggatattca gctattaatg caggcaagag
    70741 tattctgacg gaatcaactt cgcccacccg tattttctgc ttcaattccc cgatattttg
    70801 ggcaaatata aaagccggaa tcaatatgag aaaaacgaat cgtttcatac gtccgtaaaa
    70861 taggtatcag ccaaaccgca aaccggacaa atccacagaa tatcagaact ccgattaccg
    70921 caattagagc agatatactc tttctgatca accatttttt caattatgtc atttaggatg
    70981 gctgtagctc gatttaattc agcctgtcca acaagcagtt tagccagatt aattttggcg
    71041 atggacaaac cgggattttt ttcgactaaa tcttccagga tagcgatagc tttttttaca
    71101 tttcccttct tttcgtaaaa atcagcgaga gcaatcaagg ttttgggatc atcaggaatt
    71161 cttcgtagga gattttcata aaaactttca atattgccaa aattcccgag attaaataaa
    71221 atcgtctgca gaggtttaaa aatcaaatag gctttgtgcg gcgctacgtc ggcaaattta
    71281 acccactctt cgacggcatc ttcttcccga tgatccagaa cataagattt accaatataa
    71341 taataagccg cctcacaatt tggatcaatt tttagcgctt tccgaaaaat taaacgggct
    71401 tcatggtatt cacccgcctg gtatttgact aaaccttctt gaactttata aagagccaga
    71461 agccgatcgt tacgttcttt ttttaattcc aaagccatcg ccagatattc agatgctttc
    71521 tcccagcgtc ctaagtcccg gttcaaaata tgattctttt ctaaagccca gatatttttc
    71581 ttatcaatac tcaaaatgtt ctcgacataa tttagcgcgc gaattttatc accagcggca
    71641 atgtaatctt ccactaaatt acgcagtatt tccagacgtt ggatattact aatttcctgc
    71701 cgatagagaa gcgattcatg aatttttata gcgtttttag gcttaccatt cttgcgcatc
    71761 agaataccca aattgatata agcgtcaata ttatcggtgt ctgcccggat gacatcccga
    71821 aacaagcgga ttgcgacatc atcatcgccg tgtaacatat gattgagcgc catagcataa
    71881 aggctggcgg aatctttgga ccggcttaga cgacgccgac cagtcagatt aattacgata
    71941 aatccaataa gcgcaatgag aactagcgtt aaaagtatat ccaataaaga catattaatt
    72001 cctgtttatt cggtttttga attatcggct tctgtttcgt caattccctg atttcgcagt
    72061 aaatcaactt ccttcttcaa ctttttataa tcgttcgata aagcgcgtag ttcattctta
    72121 gtggtaagaa ttttaatacc agcaattaaa aagccaatca caagtcccag tataaaagtc
    72181 aaaaatacca gaattagaat atccatctca actggagatg ctttccagaa aaactggatt
    72241 ttcaccggtg gattcaaatt tgagttctga ataacaaaaa acacgattaa aaacatgatc
    72301 aaaagtgtta caacccagcg taaaaatttc ataatcacct caaaatttaa atgattttac
    72361 caaataaaga cactcccata gccttttcga tttggacatt acaaaactca ccgatttttg
    72421 gatgtccttc ttttatcacg acgattttat tagattcggt acggccgatc atttcgtccg
    72481 gatttttctt actggttccg tcaattaata cctcttgaac cgtacccact aaggctttat
    72541 tacgttccaa tgtatgactt ttctgaagtt gaataacggc tttcagacga cgagattttt
    72601 cctcctccgg aacatcgtca gtcattttag ccgctttggt tcccggacgc ggggaatatt
    72661 tgaacataaa ggctgagtcg aacttaacta tctgcattac ctgcaaagtt tgctcaaaat
    72721 catctgcggt ttctcccgga aaaccaacaa taatatcggt cgtaagcgca acctgaggga
    72781 gaaattctct aattaagtta actactttca gataatgcac ttgatcatag gaacggttca
    72841 ttaacttcag aatccgggat gaaccagcct gaagaggcaa atgaatctgt ttacaaatat
    72901 tagaatgaga ttgcataact gctaacattt tttcatcaac gtcgccggga tgaggcgagg
    72961 taaaacgaat ccgacgcaaa ctcggtatag cggcaactaa ctcaagcaat tccggaaagc
    73021 gatttgctcc ataatggtaa gaattgacat tttgccccaa caaggttact tcgcggtaac
    73081 catcatttaa cgcttgtcga atttcatcaa taatgctctg cggtgaacga ctgcgttccc
    73141 tcccgcgtac atacggcacg atacaaaaag tgcaaaattt gttacagccg cgcataatcg
    73201 agacccaggc attgaccacg ctattgcgag aaggaattag ctcatcatat acttccgtgc
    73261 gcgatagatt cacatccacg ataggcatct gttcctgatt caaatattcc ggtaaattcc
    73321 gataggaatc tggacctaaa actaaatcca caaatttgtt ttcagtcagg atttcgtcat
    73381 gagctcgctg agcgacacat ccgatcagtc cgagcaacag ctccggtttc tttttcttta
    73441 gacttttaaa ttgcgttaat ctggcaatcg cccgcttttc ggcattttct cggacactac
    73501 aagaattaat gagaattaaa tcggcctctt cgggaatagc cgtcggctga taattagctc
    73561 cgcgcagtaa gccaataacc aattcggaat cagcttcatt catttgacag ccgtaggttt
    73621 cgatataaaa tttgcgggct tccatcgtaa ctgctgaaat ttaaaatata aaaccggttt
    73681 tagaaatgaa aactaagaaa tttatatgag ggaatgaatc tgcgcttaac gaatgtatcc
    73741 agagaagaaa aagtcaagcg gatttttatt gatattaaac tgctttactt cgtagtgcaa
    73801 atgcggtccg gtcgtgcggc cggtagtgcc gacctcgcca attttttggc cgcgtttaac
    73861 ggcttcacca gatcgaacta atattttcga aagatgagca tagagggttg aatatccgta
    73921 gccatgattg acttcgacca cgtaaccata tccaccttga taaccggatt taccgacgac
    73981 tccatcagcc gtcacaaaaa ctggagtccc agttggcgtc gtaatatcta aaccataatg
    74041 gaactcacgc cgaccggtaa acggatctct tcggtaacca aaaccatcat tgataaaacc
    74101 ggtataaacc ggtcgaatgg acggagtgga ctgaatctga tcggaacgat ttttgaaagc
    74161 atcataaatc gtttcataac tgatgcgctc aaatttaatt tcccgcgcca ggcgatccag
    74221 attattgact aattccgaaa cttttatatc actctcaggc aggagctgat ctaattcgtc
    74281 cgatttgggc attaaacgac caccaacacc cagccggcgg atgtcctgat caattacggg
    74341 gagatcaccg taggttcgga gagccttgtc tttttcaacc agcatgctaa tatccgattc
    74401 cattttctgg atgcgattat tcaagtcgta aaaggttgaa accaaccgat tattgttttt
    74461 cttaagacga gttaacctag attgataaac cagattagaa aataaattgg cgcctaaaat
    74521 aatgacaaaa ataaggagaa aaactccacc ggcgagaacg gcaaccaact ttccccaact
    74581 gagcgaaatc gaatgcaccc gttttcgtgt gttcactaca aaaaggagtt tgggtgcttt
    74641 tttcattttg ttagtttatt tttcgcccat attataaaat tcattgtgaa ttttaaaaaa
    74701 tagtttttat taagtcaata ttttttttag gtaattatta attaagtaaa tctcacttcg
    74761 ggtttgctca gattattacc atcacttaat tgcaattccg gcaagcaatt atgccataaa
    74821 taactttacg aaccggcttt taaaatactt ataaaaattt agttaaaaat tggatagtta
    74881 gtctaaaaaa tcattgcttt ttcttaaaat ctttctcata ttttggccgt tggatgtaac
    74941 tatgaatatc tgatacgaac gattagagag cggtttttag gaataattct cggaagatat
    75001 tcattctata gtaaatattt tttaaaatta caaattaagg aggaaagtat gaaaaaaggt
    75061 gtctatgttt taataatgct agtagtgctg agtacgatag tggaggtggg gtggggacag
    75121 gtaagtatta ctcaatttga aactgcatac aatcaagatt tcaattcatt agctaatact
    75181 aaaacggata atgcctggac cgataattcc actatcagcg ggtggtattc aaacaggaca
    75241 gagtacatcg ccgataatgg tagtagtaca actggcggtt tacatagcta tggatcggcg
    75301 gatgccagtg agagagcatt aggtggactt tcatctagtt cggcttcccc cgcttttggt
    75361 attcgtttag taaataacac tggaaacact attacttcat ttaatatttc ctataaaggg
    75421 gagcaatgga ggcaaacggc gaattctcat acattagttt tttcttatca agtagatgca
    75481 acaggaattg aatctggaac atggacatct ttttcagatt tagattttac agctttgaaa
    75541 actggtacct ctagcgctct tgatggtaat gctgaaggta attaccaaaa tgtatcatca
    75601 aatgttatca ttgaagtacc taacggacat gaaatatggt ttcgttggct aaaaactgga
    75661 actaatagtc ctggtttagc tattgacgat ttatctataa cagcttatga aataaaagta
    75721 cctactatat cggcaaatcc tagtagtctg tcaggttttt cttatgtcgt tggctctggc
    75781 ccatctgccg agcagacttt tatggttagt ggaacgaatc taacggcaga catatcaatt
    75841 tcggcaccag aacactttga aatttcaaaa acaagttgtt cggatttcac ctcatcgctt
    75901 acttttgctc aatcagacgg ttccgtgaac gaaaccatta tctatgtgcg attaaaagcc
    75961 ggcttggcaa ttggctcata tagttccgaa aagattacct gttcttgtga gggggccgac
    76021 gatcgaactg taacctgtag tggcactgta actagtccac taccaaatgt ttggattaat
    76081 gaaattgatg cagatcaagc aagtacagat tctttggaat tcattgaact atgcggaata
    76141 tcgggattag atataactgg ctattatatt gtattatata atggttccaa tagcaataaa
    76201 tatttgaccc atactgttgg tagttttaca ttttctaacg aaataaacgg ttatggattt
    76261 tatgttgcag gttcaaataa tataattcaa aaagatgata cccttggtaa tggagaggaa
    76321 aatttaattc aaaacggaga tcctgatgca gtcgcattat tcaattcatc caaagaatta
    76381 attttttggt attcatatgg tggtactttt ataggtaatg cttttgatga ttttccaccg
    76441 gtctcatttt atgttagtaa atcatctatt gatattgaga caacttctat gggtttttta
    76501 aaaaatacta aacctgaaga ttcagaaact ggaaataaca atttactacc aacaccagga
    76561 gctgctaata ccgatggtac aaccgatcaa tctctccccg tttctctgac ttctttttct
    76621 gcccagtcgc gtaaccagtc cgtggtgctt tcctggcaca ccgagtccga aatcgaaaat
    76681 ctcggattta ttatccaaaa acggttaccg gaat
//
LOCUS       DAOSMP010000003        75224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_358_length_75224_cov_17.900690, whole genome shotgun sequence.
ACCESSION   DAOSMP010000003 DAOSMP010000000
VERSION     DAOSMP010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 75224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 75224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75224
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_358_length_75224_cov_17.900690"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            428..1498
                     /locus_tag="PKX09_00745"
     CDS             428..1498
                     /locus_tag="PKX09_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16392.1"
                     /translation="MARKIVHIIGTGTVGAPLIGVLLGHLEQLGIEEISFEPNLKAIE
                     NKAFLNGLVARGAKLCVREEIADEFISVGCKIDYTSKEAIERAGVIIDCSPGDEPLKN
                     KAEIYSRLDGEPKFFIAQSRAAGFGLDYAYGINDEALVSSEVQFVRVVSCNAHNIAAM
                     IKTLAFDEKGENYLDWGRFVCIRRASDISENKPFIPAPQISLPQDDNYGTFQALDTAS
                     LFRTIGVNLDIFSSALKVNSQYLHIVNFDLKLKQPVTLPLVINKLESNSLIALTNKTQ
                     SSLVFAFARDMGFLGRILNQSVVSVPSLTVKGDREIIGFCFSPQDGNSILSSIAATVW
                     YFYPHSYRDKLQNLDDLIFAEV"
     gene            1660..2292
                     /locus_tag="PKX09_00750"
     CDS             1660..2292
                     /locus_tag="PKX09_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU16393.1"
                     /translation="MLIEQIITQQFSENVWLVGDPQTRKGLIIDPGDHASEIISKISA
                     MNIQPLAILNTHAHPDHLAAAAEVQNHYNIPFYLHAKEKAVLKSAIPFGLVLGIRITR
                     VPSQVIYLQDEETLKIEPFEIRVIATPGHTPGSVCYLIADRLFSGDTLFRNSVGRVDF
                     PGSSWQELKRSLEKLKNLPPATEVYPGHGMNTTIGQELSHNPYLKNGEWR"
     gene            2289..3683
                     /gene="tilS"
                     /locus_tag="PKX09_00755"
     CDS             2289..3683
                     /gene="tilS"
                     /locus_tag="PKX09_00755"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOU16394.1"
                     /translation="MKPTTLQRKFVEYVEQNHLLNDVRTVVAAVSGGVDSMCLLDLLM
                     SEKKRWDIEIVVAHFNHQIRGPEADSDENLVRQFCDNHSLPFFSAAENVPKIAHAKRI
                     SLEMAGRELRYAFFQKIAADFPNSIIATAHTLDDQAETILLRMLKGTGLQGLSGITMR
                     RENIRRPLLFATKAELYDYARARELPFAEDHTNLCNDYERNFIRNQILPTVQHTLNPN
                     IRATLANLAQNLGEAHQFIVEFAEKALESLIIKANSEQIVLDKSSLKTYFIAVEKEII
                     WQSLQRLYGTIKPLNFKQMNQLIKLVNEGETGRRMNLGGEITVLIDRERLILKRNRSS
                     NWSSSPIIPGEKIKNEFFTFSSELMDVSNFKKGKRRSGVEFIDFDLVGNNLGLRHWQR
                     GDRMIPLGMNQSKKISDILVDLKVPLTDKNSIPVFYSEYKIIWLCGLTMSDEFKVTSK
                     TKTVLKLIYKGKKI"
     gene            3680..4237
                     /gene="hpt"
                     /locus_tag="PKX09_00760"
     CDS             3680..4237
                     /gene="hpt"
                     /locus_tag="PKX09_00760"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOU16395.1"
                     /translation="MKKTITLDASYGPYAGQVIEELLTVAQINRRVTELAAEISAMYQ
                     GTVPIFIGVLNGSFIFFADLFRKLTIDGEVDFIKISSYADQTRSSGTILLRKDISCDI
                     TGRDVIIIEDIIDSGLSIKFLKRRMEESNPCSVRFAALLMKEDARVDFPIDFVGFKIP
                     NKFVVGYGLDVAQRLRNLASVYAFK"
     gene            4260..6335
                     /gene="ftsH"
                     /locus_tag="PKX09_00765"
     CDS             4260..6335
                     /gene="ftsH"
                     /locus_tag="PKX09_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOU16396.1"
                     /translation="MSENNYPKNQSSKNLNEPRKPRQPRRRPPEPPKPNKDDFRWKKA
                     SRTSLMWIIIILSSIVLLQILGRGAQEEVVITYSQYKELLDQKLIKSAVIIENEFHGT
                     LTQKVPLVVGGRTIEAEKILVNLRFVDSEMLKSWDEAGIEYDFKVKNTKWTNFFINIF
                     PLVFIILMTLFFLRRMQGQNNKGIFGFGKSKARLWTSDRPKVSFNDVAGCAEAKEELQ
                     EIIEFLKLPSKFSRLGGKIPKGALLLGPPGTGKTLLAKAVAGEAGVPFFSISGADFVE
                     MFVGVGASRVRDLFEQGKHSAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLNQLLVE
                     MDGFDENTNVIILAATNRPDVLDTALLRPGRFDRQIVVDMPDLVGREGILKVHTKKIP
                     LADDVDLSVLARGTPGLVGADLANLVNEAALLAARKNKKAVEMIDFEEAKDKVMMGVE
                     RRSLHISDRERRLTAYHESGHVLVAVNIPEADPIHKVTIVPRGRALGITHQLPMDERH
                     NYSRSYINSRLSILLGGRAAEKIIFNELTTGAGNDIETATELARKMVCEWGMSDKLGP
                     LTFGKKNEEIFLGREIATHRDFSEETARLIDQEIREIVENAEKQACEIIKKNIDLLHK
                     LALALLEKEVLDGAEIAKILGNSEVNSANIFVNKSDDNMTKPATKAKTGSRRRKSEKE
                     KGANTDQAA"
     gene            6367..7512
                     /gene="folP"
                     /locus_tag="PKX09_00770"
     CDS             6367..7512
                     /gene="folP"
                     /locus_tag="PKX09_00770"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOU16397.1"
                     /translation="MESVDVDPAGIAIMVRKSEFLILKTDPIIAPAANILKQQILSVG
                     GDCAVAREVPTGQAPPTPVILMATRRQYERLIINLKQQCFGLDKLGTEIEGFLARISA
                     PRKFHIGQQEYQTDQKTLIMGILNVTPDSFYDGGRYAEMSAAIETALKMEADGADLID
                     IGGESTRPGSDPIPAEVEIARLKPVLEQLQMRLKIPISIDTYKSAVAEWTLNNGALII
                     NDISGLNFDSQMAGTIAKHNAGVILMHIKGTPKNMQADPHYTNLIDEIMKYLSDSVQK
                     ARTAGIDGSKIFIDPGIGFGKTAEDNYRIMRYLAEFQSLGFPILVGPSRKAFIGQVLN
                     LPADERLEGTLAAVTAAVLNGADLVRVHDVKAAVRAVKIADAIKGKS"
     gene            7513..8289
                     /gene="cdaA"
                     /locus_tag="PKX09_00775"
     CDS             7513..8289
                     /gene="cdaA"
                     /locus_tag="PKX09_00775"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOU16398.1"
                     /translation="MTLFTIKFLSVTIWDVIDIIVVYIIILKLYQFFKGSRASQMLIG
                     LAIILLTSFIARALNLQSLSWIMGNLQTIWAIAFVIIFQPELRRILIYLGQSRFVRRF
                     LSESKSKTIEAVIEASRELVRRKWGGLFVLVREVGLKPIKERATPINAEVSASLLVSI
                     FNPGSPLHDGAVLIQNDIIEAAGCILPLSDSPDLDPQMGTRHRAALGISEESDAVVVV
                     VSEETQKIAVAYQGILYRNVDEDSLRALLSRLFFRGSNNS"
     gene            8311..13221
                     /locus_tag="PKX09_00780"
     CDS             8311..13221
                     /locus_tag="PKX09_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOU16399.1"
                     /translation="MSIKPVPFYLSHNIKHPLRILFCGFILLISQNLLWSAVSDIEIV
                     EKWLNATTWQWQAELPMPKFPVDEKNQTCAIEISGLENLARDGYPSIPVYQKIINALP
                     DEIAFTVQTGLEKVVPLPAVMSISQDIPIAGEGTNQEKIILPKTKNFSAYPMRLVEIE
                     FLGYLKGIPLSSVKIFPCQLIEQGKRLKYFGSVTVQVQVKPGTRLQKSVATDEQALLK
                     ALDIAPGSIRKIAKQSPTLAKPAVTFPAGKVLAKLIVDEDGIYRVSCRALSDSGIALK
                     KVDPRTFRLFNQQHEVPIYVYGESDGIFHNNDYIEFFGQRNRNSVADYEYDPFTDKNV
                     YWLTWGENYGLRYAEESVQVNSPIDETVVPIDYLYTAHIEVNAAFERLGQVDVDQPTH
                     VRDHWFFDSGIFGGTAREYVFHLSYPNPNQINNFNAQLRMQGLTYGDAGDHQADYFIN
                     NIKVASGAWNGQKAYTLENSASQVLHNNFLKHGENVLRVQVSGADPTDKYSKVAVDYL
                     KVKYFRLYKADEDELDFTRPESSIDGLYTFTLSNFNTSDISIYKVGKSKLRDFTTEYI
                     KSTNSYRVMLQDYVRDDTTLYWAACGEALKFPLSVKLDTIIGLSSIPSGAEVVVITRQ
                     EWVNNLSKLTDFYASIGLSSQVVGIEDIYNEFNAGIISPFAIKKFLQYVCYNWNPVPE
                     YILLVGDADIKEERSIPSYFFQSYSYGACASDFWYALIDDDDVPDYAIGRWPCSTIEE
                     LEALIDKRLNYTKHNLIGPWNNELLFIAGQENVFKNQSENMIQRQISKEYNINRIYVN
                     PASVGSRFYGGSDTLLYLFNNGITLGNFMGHGGGAVWGDRSLFNSSHIPELENLDRLP
                     FLTSMTCFTGDFTNVTGLGEMLLLAENGGAIGLWGASSVGWIKNDYLLAKPFYDVIFE
                     PGMTVGKAIQTAKIRYQAEAFDYLRLSLIYSYNLIGDPTVGLPFPEQSTLLNLSQTNP
                     APGDTVILRGDLPFEAGKMYIQLFDSSKYAVYEKPAEVPFSSSNLHHEIILPTNINPG
                     NTYVNYYIESKTAKQDAHGATLFDIKGLTFYGFDYAPSSPGEKTPITLSIYTELSNVQ
                     SFFCEIDTVSAYEYLDGDGIEHIISFQNDSVLLSIRMGEVESVANKWQTTQVFQVGTP
                     GKLVAVRFVAIDSSGNRTVSPAYSIRIKSLPDLRPIGIAQGGERFPELIAEVSYNGND
                     TLSAVVKAERRVDEKFELFGSKEVVFLPNRATSVKLPGILGKDTVIFRITVDPENTVA
                     ESNEMNNSLSQSMVINTFAVLPTCGTTYDGSQSDTILVGSVFKVAIAPLSVADSAVLI
                     LQIDTNLTIASQPKFSLVSPDSGFEALGLKATGGNSTSTSLLAVNVTVDLSNCPAASQ
                     IDDVGIGKWNPSLRIWYNEEGQKEGMKIKTTSTIPGTFSLITNTDTKPPNLELNLEGQ
                     QFFQNSYVSDRPSISIIGEDDNGVRFDAEGIKVIIDNNEVALENLHAPDTVSSGHYFS
                     VQFRPTFTHGNHSMEVTLKDAAGNSTTESVGFIVAEELKLIDYGNYPNPFRNRTTFIY
                     ELTQRVNSLKIKIYTTSGRLVRVLDSENVFESGLDLNEGGYHEVVWDGLDADGNFVAN
                     GIYFYKISAKSGNKTVTKTGKLAKAR"
     gene            13218..14228
                     /locus_tag="PKX09_00785"
     CDS             13218..14228
                     /locus_tag="PKX09_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16400.1"
                     /translation="MKRILIIGIILIGLSGRLVAGEYADAFLELGVSARASAMANAIG
                     ALDFSETAFITNPAGISYIRQARLGLMYTSQFGLADHNYIGLAMPAGVHYSLALSWIR
                     YGVDDIPIRPDIFTTITDKAERRDSIISIYNGKLPTFNDVENAVFVSLGRFNSTIVDL
                     GWRYSKFNLEFPIGINLKFIHKKYYDLEGFGLGVDIGGRLRVDGEELLDVSNLGMLSV
                     GMALKDVTGTTIYWNTKSQDRIRLTPALSFALEQPIKPLKLIINLVSEKQYRYHDKTN
                     YGVEIIYANSLALRAGLKNSGISLGLGLNFKVMKKLINLDYSFYSHDLGASHRVGLGI
                     VI"
     gene            14234..14989
                     /locus_tag="PKX09_00790"
     CDS             14234..14989
                     /locus_tag="PKX09_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16401.1"
                     /translation="MTTSNQINLMVALILLLNTCGKNLEDPDPPAQPEWVAKSLPYDT
                     TETGIDAVPNGDFIQLEWYTGDEDDLSSYRIYRASGDIENKFDLIEEIQSDLMAGSVN
                     IYLDENVSINTPYFYFLRAVDQAGNLSLRSDTVEYKLVVKVNLRGLFDTTDVNPTFKW
                     SDAGSAASEYVIRVEQFNPNRVMCLSATSRQSYTDEVQSLKYGTGIIFYSAQDTLSRG
                     VDYRWRVDAVATFNREGDEVAGSESNWGYFKIQ"
     gene            15005..15523
                     /locus_tag="PKX09_00795"
     CDS             15005..15523
                     /locus_tag="PKX09_00795"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOU16402.1"
                     /translation="MKRVSLFMILTLLSVQLYSQESQTITTRSGRTADRLSASKYILS
                     TGSDAMLMTVKIWGEVTRPGLYDVPIGTDLIDLISAAGGPTTRANLSKVKLIHGSPDE
                     EENYVTIVNIKKFLETGDVDMIPEIKPSDTIVVPVKITQHFLTSLSWTQQLLSLFSIY
                     SMILYYTSIANR"
     gene            15541..16008
                     /locus_tag="PKX09_00800"
     CDS             15541..16008
                     /locus_tag="PKX09_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOU16403.1"
                     /translation="MIDSIDVQILDILQKEGRIQRNKIAEQVNLTIPAVSERMRKLEK
                     KGIIEGYHAKINSAAIGKDVTAFVFVMTSPSKNYDEFVRRSREESDIIECHSITGEGS
                     HLLKVQTDNTSALEKLLSKIQSWPGVLQTRTYIVLSSYKDFSSLKPASSLVKK"
     gene            16078..17194
                     /gene="prfB"
                     /locus_tag="PKX09_00805"
     CDS             join(16078..16140,16142..17194)
                     /gene="prfB"
                     /locus_tag="PKX09_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102881.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOU16404.1"
                     /translation="MLSDIQVELDNIAPRIDELRRFFDVESKKNELNTERQKTTASDF
                     WSDPAQAQTTLQKIRGLESEIDNFENLVKYQKEITDFVDLAAEEQDDSVLAELEKMVN
                     ELISKVEQLELETMLDEPDDEKNAILTIHPGAGGTESQDWAHMLMRMYLRWMERSGME
                     ADVISILDGEEAGIKEVSIEVKGRYAYGYLKAEAGIHRLVRISPFDANHRRHTSFASV
                     FVYPAIEDDISVEINQDDLRIDTFRASGAGGQHVNKTSSAVRITHIPTGIVVQCQNER
                     SQFKNKDSAMKILRARLYQLKKEEKNKEKMEIEKNKKDISWGNQIRSYVFQPYTLVKD
                     HRTKYETGNIQAVMDGEIDEFLRQYLLFNVKNERAKK"
     gene            17191..18690
                     /gene="lysS"
                     /locus_tag="PKX09_00810"
     CDS             17191..18690
                     /gene="lysS"
                     /locus_tag="PKX09_00810"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOU16405.1"
                     /translation="MNKEEQATGRSLNELLAIRREKLQAIREMNFRAYEYEFQKTHSA
                     AEITQNFDELTEKVVVKVAGRLMAIRKMGRASFGHIQDESGRIQIYVQENSVGQQGYK
                     LFNLLDIGDIIGITGRVFRTHTGEITILVEELQLLAKNLRPIPIVKEKDGQIFDAFSD
                     KEQRYRQRYLDLMVNPEVREVFKTRSKIIQWTRDFLNEHGFLEVDTPILQPIYGGANA
                     RPFKTYYNALEREFYLRIAVELYLKRLIIGGFEKVYEIGKNFRNEGLDRSHNPEFTLL
                     EFYQTYVDYNYMMDFVEQYFHYLCSKLGESKFPFGEYEIDFSQPFSRRKMFDLIHEYV
                     GEDITQYSEAELKSLCLKKNLELKPGDNYGKYIELLFSNFVEPHLIQPTYVIDYPKAI
                     SPLAKNKRDGSPNLVERFELYIAGHEFVNAFSELNDPIDQRERLEQQNRLRESGDEEA
                     QTMDEDFVTALEYGMPPTGGVGIGIDRVVMLFTNQVSIKDVILFPQMRT"
     gene            18703..19923
                     /locus_tag="PKX09_00815"
     CDS             18703..19923
                     /locus_tag="PKX09_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU16406.1"
                     /translation="MSVPFFIAKHHLFSRHKIGYISFISIIATISLAVGVAALILTIS
                     ILNGFEQEIKTKLLGFDSHIRLRLLYQDSMDSTLAIRSELEKMPEIECFVPYIHGAAM
                     IRKGQETEGIIVEGIDEVHIRQTLAVDRFIKKGELRFNLPDGREGILIGAKLARQLGV
                     DIGDKVYLFVFQPEEGYIKRPRIVTFTVTGLYDSGIADYDDVFIYTSLQAAQKLYDLG
                     EKFSGYQIRLKDQSKVEAVTEYINKTLGYPYNALSWNDLHSNLFEWLRIQRIPILLIF
                     SLIAVVAIFNIVSSIMMIVIEKTRDIGILKSVGMNNSQVKRIFLYEGLFIGVVGTLAG
                     FGLSILLGYLQNRFNLISIPADVYFMSSLPVLLELRNFLIIGLIAIASALLATLYPSY
                     KAVKLTPSEATRYE"
     gene            19916..21139
                     /locus_tag="PKX09_00820"
     CDS             19916..21139
                     /locus_tag="PKX09_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU16407.1"
                     /translation="MNKNFLNWIALRYFRSKKKTGLISFTSYISIIGIALGAFAMLVA
                     LSVLNGFEAEITNRVIDIESHLRISGRNLQSAQIEQVKEALSGVPVREIYPFVMKKSI
                     LSSEQMEAVVRIKAIESATLEQQLSGPRTMMRGGANFLSATSNLPGIVVGYQLADKLG
                     VYPGDTLNVINPTSLGSGLNIPIIGQFAVTGVFQLDLFDYDENIAFIELKVGQEIFEM
                     GDNYSGIDIRLENYRYVEKAKQQLVAELGNEYDVASWEDLHRTLFGAMKLEKYGSFLA
                     LSFIILVAILNLTSALVMLIMEKIQEIGMLQALGMNQTQVRQIFLRLGFLIGSIGLTV
                     GIGLALILCLAQQHFQFIPLPSVYYIPYLPVQVQWSDVLMIISAGLIFIYCGTLYPSW
                     RVGKLMPLEAIQYEK"
     gene            21129..21830
                     /locus_tag="PKX09_00825"
     CDS             21129..21830
                     /locus_tag="PKX09_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU16408.1"
                     /translation="MKNENDKTLLTVKNLSKSYWNDGLEIAVLRDIDLTVKEGEVVAI
                     VGPSGIGKSTLLNLLGTLDRPDSGVIEYEGLNPFDLSDTEIARFRNTHIGFVFQFHHL
                     LPEFTALENVMIPAMIYNRDVRKAEEMAREMLARVGLAERATHRPAALSGGERQRVAV
                     ARALINNPLIILADEPTGNLDAMNSELLIELMLELNRIYRRTFIIATHNQTIAEKSHR
                     ILKFTKDSLLIEASI"
     gene            21978..24446
                     /locus_tag="PKX09_00830"
     CDS             21978..24446
                     /locus_tag="PKX09_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOU16409.1"
                     /translation="MKENFSKIIQIIVKAARYEAGRMNHSYVGSEHLLWAIINMERSV
                     TLEILMALDVDIKALQTMLTDSLKAIGGTATIGQIPFTKQVERILRNTYNEAKELGAE
                     VVGDEHITLSILKEKEGISYEILNSFGVDYYNFRAEISGNMDEPLPVTKSEKKSSRTP
                     ALDHFSRDITEKARTNQLDPVIGRDQEIERVAQILSRRKKNNPVLIGEPGVGKTAIAE
                     GLALRIVAKKVPHLLANKRIIDLDLAAIVAGTKYRGQFEERMKTIMQELEQARDVILF
                     IDELHTIVGAGGASGSLDASNMFKPALARGELQCIGATTLDEYRKFIEKDGALERRFQ
                     KVIINPPSKEESLEILKGLRERYEAHHHVKYTDEALWTAVELSDRYITDKFLPDKAID
                     VMDEAGSRVHLRDVIMPEEILEAEKKLEEVRNQKDNAVREQQFEKAADLRDSERRVKK
                     QLADLQQAWEKSGEKQTLLVTAEDIADVVAMMTGIPVNRIAESENQKLLTMEDELRKS
                     IIGQDDAIRVVSSAIRRARAGLKDPRRPIGSFLFLGPTGVGKTEFARVLARFLFPGTD
                     ALIKIDMSEYMEKFAVSRLIGAPPGYVGYEEGGELTERVRRQPYSLVLFDEIEKAHRD
                     VFNILLQIFDEGVLTDSFGRRVDFKNTIIIMTSNAGSRSLQSSAFGFSKADPLSEYEA
                     MKKRLLEEIQHVFNPEFLNRIDDPIVFKPLTREDVLAIIDLQLEDTRRNLGASGTTLE
                     ITQEAKELILTQGFKPEFGARPLRRAIQNMIEDPISELILAGTLRRGSKIVVSVTDNK
                     IVFTQPEIEFKISSTKITSEPVSN"
     gene            24587..26509
                     /gene="dnaK"
                     /locus_tag="PKX09_00835"
     CDS             24587..26509
                     /gene="dnaK"
                     /locus_tag="PKX09_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOU16410.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGGKPRVLENSEGRPVTPSVVVFKK
                     DGEIVVGDTAKRQIVTNHDRAVYSIKRFMGRLYKEVQREREEVPFKVVEGPNGDARIR
                     IGDRDYSPPEISAMILQKMKQTAEEKLGEKITDAVITVPAYFNDSQRQATKDAGKIAG
                     LEVKRIINEPTAAALAYGLDKKKEEKIAVYDLGGGTFDISILEIGDGVFEVKSTNGDT
                     HLGGDDFDQRIIEWLISEFKKKEGIDLKKDPMAMQRLKEAAEKAKIELSSNLETEINL
                     PYITAGSAGPIHLMEKLSRAKFESLVDDLIERTRKPCQQALEDAGLKPEDINEVILVG
                     GSTRIPKVQDLVKDLFKKEPNKSVNPDEVVAIGAAIQGGVLAGEVHDILLLDVTPLSL
                     GIETLGGVTTKLIERNTTIPTRKSEIFSTAADNQTSVEINVLQGERAMAADNKSIGRF
                     HLDGIPPAPRGVPQIEVTFDIDANGILNVTAKDKATGKEQSVRIQTSSGLTEEEKEKM
                     VRDAQLHEEEDRRRKEEVDTRNQVDALIYQTEKNMKEYGDKLQPEEKGRLNNALEDLK
                     KAQNSGDVKTMKSAIEAMNQAWSEVSSKMYERVRTETPPPHTGQTYEQPHEETTGQKD
                     ENIQDADFEVVDDDKK"
     gene            26630..26702
                     /locus_tag="PKX09_00840"
     tRNA            26630..26702
                     /locus_tag="PKX09_00840"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26663..26665,aa:Cys,seq:gca)
     gene            26737..26821
                     /locus_tag="PKX09_00845"
     tRNA            26737..26821
                     /locus_tag="PKX09_00845"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26771..26773,aa:Leu,seq:taa)
     gene            27091..29571
                     /locus_tag="PKX09_00850"
     CDS             27091..29571
                     /locus_tag="PKX09_00850"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOU16411.1"
                     /translation="MESSKKQSVINEKPTPIKGLTTEEALSRLKKYGPNTFPRSKPPS
                     AWSILISQFKSPLIYIILVAAVISLIMGELTDFIIIIAVVIVDVILGFFQEFQAQRTY
                     TALNKLLKPTTTVIRDGERQEIDVTKVVPGDIVILNAGERIPGDGEIIEGARLSVDEA
                     ILTGESEPIKKSVEAGSNQVFMGTTLLTGRGVMRVTQTGVRTELGKIAKSLQEHKEEE
                     TPLQVRLKSFSRTLTIIVVSFTTIIFLVGLLLKRDFLDMLRTSVILAISAIPEGLLIA
                     VTVILVLGMRKILKRQGLVKKLLAVETLGSVNVICTDKTGTLTEGRMRVTRTDFVDNE
                     RAIQIMVLCNNLEGPVEMALWEYAKSRLPNDPQTLVEESQRLDEEIFTSETKFMITGQ
                     HLPDGDYHFLKGAPEIVLNMCQINDGERQRILAQIDQWAGEGLRLLGLAYRDYGILED
                     HTGYRWVGLIGMEDPIREGVHEAVLLAKHAGIQVKMITGDYRRTAERIASNIGLLQNG
                     EETLEGEELAQLNDEELAKRIYPTAVFARIRPHDKLRIVNALQKHGDITAMIGDGVND
                     APALKQANIGVVVGTASDVAKETADLILLDNNFRTIVAAIEEGRVIFENIRKVVAYVL
                     SNSFAEVLTIFISMILGWPAPLLVAQILWIHLICDGPADIVLGFEPKENGIMDEKPKS
                     LKEPILPPLGLFLIGVISIGSALAALLIFGHYFKIHGDADEGRSLVFASLAINSLIYI
                     FAYRSMRKSLFKMNSILKNKPLIFAVIFGMVLVFLPFVIPSLSQALDVVPLDLKQWLL
                     IFTIALMLLIAVEIGKAVINWWHNKKIL"
     gene            29727..31172
                     /locus_tag="PKX09_00855"
     CDS             29727..31172
                     /locus_tag="PKX09_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOU16412.1"
                     /translation="MAKIDPNSDYYSELSQFTIESDVDAILWIDAESTIYRVNQAACQ
                     LLQYSPEELVGKNLDDLIDIQNDWNKTDPHRFLDQIDIIKQNGVTVIERNFRTKDGRI
                     LTLEISAHLFVFKNNEYICVFARDITERRKAQEELQKTLKELEFLKNKLYEENIYLQN
                     EIKLTHNFEEIVGKSLSLRKVLEKVEQVAATDATVLIQGETGTGKELIARTIHHISSR
                     SSSPLVKVNCATLPAALIESELFGHEKGAFTGALYRKMGRFELANGGTIFLDEIGDLP
                     LEIQSKLLRVIQEGEFERIGSNKTLKTDVRIIAATNRNLETAVQKGEFRKDLYYRLNV
                     FPIHVPPLRERPEDIPLLIHHFVQKYSKKIGRKIDIIPAKIMRTLTSYSWPGNIRELE
                     NIIERAVIITNGRTLQIGNWFTTDAPVMNKEKIQPLREAERDYILKVLSLTNWRVSGP
                     NGAAQLLDINPQTLVSKMKKLGIHRTKPINE"
     gene            31323..31670
                     /locus_tag="PKX09_00860"
     CDS             31323..31670
                     /locus_tag="PKX09_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOU16413.1"
                     /translation="MKKENKSVKKEDTKIIDMSTFDELIHSNKPVVVEFYDEKISTSY
                     IVDSILSRLANYFQNQIEFLRLHINQHRAIADKYHIFSSPATLIFLNGKLIEVILGTF
                     SQNQIIEILNRLL"
     gene            31692..33104
                     /locus_tag="PKX09_00865"
     CDS             31692..33104
                     /locus_tag="PKX09_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU16414.1"
                     /translation="MTTFKKYVNKIALDKLVTYCIKDPQKHIPKLIDFVDKSAWLPIH
                     KSYVQGAKSIVNNPDSNWYQLAMRAIRDIDPDIIHKIVRNLFINSGFEGQGKLVEERE
                     RLGFGIPWAVLMDPTSACNLRCKGCWASEYDKSLNLDTDLMDRIIREGKELGIYFYIY
                     SGGEPLIRKNDLINLAEKHSDCVFLSFTNGTLVDEHFADELRRVGNFSLALSIEGDEK
                     ATDFRRGKGSYQKLISAMKILKSRGIPFGFSTCYHSQNWNIVGEPEYLDQMIEAGCYF
                     GWYFTYIPLGKHADLSLLARPEHREHMYYRVREARQTKPIFLIDFWNDGEYVNGCIAG
                     GRRYLHINANGDVEPCAFIHYSDTNIKNVSLLEALQSPLFLQYQKNQPFNQNHLRPCP
                     LLDNPDALKSIVLETGAYSTQHMDREDVVELTAKCQDIAQAWKLTADRLWEETLKNRV
                     QKKSGVKQYERELVDAEIHN"
     gene            33127..33258
                     /locus_tag="PKX09_00870"
     CDS             33127..33258
                     /locus_tag="PKX09_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16415.1"
                     /translation="MASDLGILGIWLFISELIGGTLVNRLILIIGGIDCYFTTITHK"
     gene            33842..34948
                     /locus_tag="PKX09_00875"
     CDS             33842..34948
                     /locus_tag="PKX09_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOU16416.1"
                     /translation="MTANSQAENLSHLTLKEAFKDDFKIGAALDLNQISGRDEQGLAI
                     VEKHFNSITPENILKWEEVHPEPDRYNFDAADQFVAYGEKHNMQIIGHCLVWFFQTPD
                     WVFEDGAGKPLSRAALLARMKEHIFTVMGRYKGRIHGWDVVNEALMIDGTLRKCKWLD
                     IIGEDYILKAFEYAHEADPEAELYYNEYDTEHKSKREALVRLIQDLQAKGARVDGIGT
                     QGHWFLDYPSREEIEKAILEISTTNVKQMITELDVGVLPFYPVDSQMVDISSFDPEMQ
                     KKYNPYPDGLPDSVQKELARRYAELFTIFLKHSDKIDRVTFWAVHDKQSWRSYLPIRG
                     RVEYPMLFDRQYQPKPALEAILKVAQDRAKLVGN"
     gene            35045..36262
                     /locus_tag="PKX09_00880"
     CDS             35045..36262
                     /locus_tag="PKX09_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOU16417.1"
                     /translation="MEKKKNLEIVAAGHTCLDLIPAFTIEGEVEKMTDVLVPGKMINM
                     GECVIAGGGPVTNAGVSIRRLGVKTELIGKVGNDDFGRLVLDWYEKNEGHFKGIKMVD
                     GESTSYTIAICIPGIDRFYLHHCGANDTFGYDDMDFNLVGRAKLMLFGYPPWMRKIYE
                     NTGAELTKILKKTKELGTTTALDLSLPDVDSYAGQVDWKAILTDWVPLSDIMVPSAEE
                     LFYFLYKDKFLEKRDKLRHKETVLDQMTVKEISTMGRDLIRMGAAIAMIKCGSRGLYI
                     YTAGQERLKKMGAAGCADLENWANRELWFPVYQEEKFVGALGSGDSAIAGFLSAFVWG
                     HSIESCLRYANAAGSMNVTVPDGLSWNKGFDDLTRRIKAGWKTKEMKINEPGWKKERE
                     FWVGPDNRGKWES"
     gene            36280..38097
                     /locus_tag="PKX09_00885"
     CDS             36280..38097
                     /locus_tag="PKX09_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOU16418.1"
                     /translation="MSKERDVFIQKKIAGMTLMEKIGQLLTFTRRGTLLTPSGIEQIT
                     RLHCGGLCLEPYAVETCKNLYWGNSQIDKNFQPPKDYFTIANNYFDGKTFGISITPED
                     YTKDLNRLQKIAMSRPAGIPLHMTIDFEGDFKNDYMSGGIRQFPGPMGMAAIGDEKLT
                     YEIGKTIARMLTAIGVTQMYSPVCDVNTNPRNPEIGVRSFSDDPEVCARHAVALIKGL
                     QDYGIAATAKHFPGRGDSGSDAHDVLEVLNLDKKRMHEIELVPFKAAIDAGVKAIMTG
                     HSVYPAFDDKYPTTLSEKILTGLLREELGFEGVIVSDAIGMAAILKQWPLPQACAMAI
                     KAGCDTILLKADDESRSQCFFGIKMAVERGELSEERINQSVARLLKMKYDQGLFESAG
                     EKDPEITREITTAKEVIDFSWDVAKKALIVMRDEQHLLPLDPQQRILVIEQRIPYEFL
                     GKDPYCHTHMFCEAMVNHSQNLILADTAFSAYEDEIKECLELAKQADLVVMTNYYARI
                     VKSGNNRLLVKKLKEAGHKVIVVTNNPYIEGTTVDADAVVCNFSGTPDSIRVSTYLLY
                     GKVIPCPTTKLPIKPDVGEKTPVKDLKAPKKHPLNLSYC"
     gene            38311..40788
                     /locus_tag="PKX09_00890"
     CDS             38311..40788
                     /locus_tag="PKX09_00890"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HOU16419.1"
                     /translation="MTVHFQGMSKTLQFPQTIALEGAPTCSIGISPEGLYNRLNDAGR
                     LILLNQNAIQFGSAPFIKITADPNAEDERDEYFMLTTRDPNIVEGPDDVPALKNYYHP
                     PVPEAKVTISLATPLAEIIYDLAKISVTRLITSPLFSGNDQTLIPISVEEFVVENTSD
                     KTQEVTLVIPRPSLVNLQEKELKPTDQDTVYICSASVKGQVHEAFNSAGIKGVVMGNQ
                     ECQEKMAIAVPEIEGVDIDTHPHFCLNHLKQDLLLNDDGSFYEKRNVIPHQDYGAAIS
                     LHFTLGPKASLKIPFAIALDFPQQRFIDGVEFERKYVKLFKNQASRTIEMIKVALENY
                     PEWLNRTLTIQNRIYELIRKNPAYRDDEEGALRLARLIFNEFSCPISNACVWVKDNGG
                     NERARFLECFDYAYINPSDVDWYSMVLLFLFPKIEEEICQGFINSILAEDPTPRFYHL
                     HASFVEARQHFEKYPEEYEGISLTQIWDTTKVKGSVAHDLGALPKGHALRNVSDYTWY
                     NNNYWIDLFPKLATRVLRNVKFTGNTAFVKENWETLKFGFDHLQELDFDGDGIPEGYP
                     GEVKNTFDNLTLFGVDAYDATIFMAGCYAMIKMAEIVKDTAAKKQYEEIFDKVSKEFE
                     KLWRDTKNSQGKRLQYYVTCFDPKMGETNTDVWMNQLDAIWALIAMGEESFIPEERIK
                     QILKTVYDNNRAYMGWAMCRTEDGGKVESEQGQDVYTTSNYVFAQLLDYYGMTEESKE
                     VYKAMDKVIFGHGNTLISPDNLRAELEQEAGESAPGPHYIVAAYPRPGAILTQIVLQY
                     IKETQKQTKNMLVDSDKLKSFAISLMK"
     gene            40852..>41395
                     /locus_tag="PKX09_00895"
     CDS             40852..>41395
                     /locus_tag="PKX09_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU16420.1"
                     /translation="MKEKTGVLSLKEKIGYSLGDTASNLFFQTFIFFLLYFYTDVFGI
                     PASSVATIFLIGRIWDAVNDPLMGAIADRTNTKMGKFRPYLLYTALPFGIFGFIMFIT
                     PDLSVQGKVIYAFITYNLMMMTYTVINVPYSALMGVISPHSEERTVVSTFRFVAAFVG
                     QFIVQFAVLRLVSVFGKGNEQ"
     assembly_gap    41396..41495
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <41496..42410
                     /locus_tag="PKX09_00900"
     CDS             <41496..42410
                     /locus_tag="PKX09_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU16421.1"
                     /translation="VQFAVLRLVSVFGKGNEQIGWQWTMAVMAGLSVVLWLVTFATTK
                     ERVKPVKGQKNPLKQDLADLSKNVPWLLIGSATIFQLLFNVIRGGSIMYYFKYFVQDQ
                     QLFLFGKPHSYSYQTLTSTFMLTGTLITIVGSLLTSFFSKRIGKSRSYWGFLGISAIC
                     SALFFFLKPQDVVWMFLLQIIASFCMGPVSVLQWAIYTDTADYSEWRTGRRATALIMA
                     VSLFALKLGIALGGSILAWILDFYGFEANVAQTARSLTGIRMVMSVYPAIAGMIGVIL
                     MTFYPLNNKMMLKIEEDLSARRQGIATE"
     gene            42801..42959
                     /locus_tag="PKX09_00905"
     CDS             42801..42959
                     /locus_tag="PKX09_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16422.1"
                     /translation="MKKIGLIMSLLIGFSLCGRQAGIHFEKMDFDTARQKAQNEQKLL
                     LTYFYTDW"
     gene            42975..43847
                     /locus_tag="PKX09_00910"
     CDS             42975..43847
                     /locus_tag="PKX09_00910"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU16423.1"
                     /translation="MNQLVYTNKEVGEYINAHYISIKINGDSLEGKQLREKYKYPGYP
                     TTIIMTSDGEEIDRIVGFDGQKDEYFQLLKDYTAGRGTLGDYLKKLDEEPDNFELNYA
                     VLNKYYDRNDTPMIRKYAQIVLELDPENTKGKKTEMLYRLAFAEYKTSGEINPLMNFI
                     NTCNDEQWLEQAYNEAVRYYQKNSDQSQVLATYESAIQRLPQSTSMMNSYAWYIFQNK
                     IADHYARGIEIARRAVELSPDADHIWDTLGQLLFAAGRVDEAIQAMQKASDLNPQEKS
                     YQELLIKYKKVLNS"
     gene            44598..45638
                     /locus_tag="PKX09_00915"
     CDS             44598..45638
                     /locus_tag="PKX09_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011397087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HOU16424.1"
                     /translation="MEIEKQDARKLSKENLEMLRNQAIRLLKQGRKQVEVAEILGVRA
                     ASISDWWKIYRTHGKKGLILRNPGPAKGTNRMLSQDQEKEIQKLIIDKTPDQLKLPFA
                     LWDRKAIRELIINLYGIKVAIRTMGEYLHRWNFTPQRPLKRAYEQRPQEVKRWLAEEY
                     PVIRHRAKQEGAEIQWGDETGMRNDSHYGRGYSPKGNTPVINLSAKRVSINMISSITN
                     RGKVRFMMYRKNMNADVLIKFLKRLVKGSNRKIFLILDNLRVHHAYVVRDWLEERKDQ
                     IEVFYLPAYSPEINPDEYLNCDLKTSVHSQSPARTQDDLTSKVTSFMRKLQKSPSRVS
                     NYFKHPKIAYAA"
     gene            45639..46244
                     /locus_tag="PKX09_00920"
     CDS             45639..46244
                     /locus_tag="PKX09_00920"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HOU16425.1"
                     /translation="MLRLMIAGLIYAPTFYPSSFESFGLKLPEMAKDLNLIIKLHQWS
                     YFLEKFSGVNLRRHIKLANKIRKKYPRVVMIEPEHYNIIDLYQASDILLTEASSTIYE
                     FMALRKHVIVCDFFKKKIEHHLLPKRIYLHRLDPEMHGIMTEFCYHIKKPQELTVALD
                     KCFQKPDPFVELREKYIADMLYKLDGRASERIRNSVLSRVN"
     gene            46276..47004
                     /locus_tag="PKX09_00925"
     CDS             46276..47004
                     /locus_tag="PKX09_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HOU16426.1"
                     /translation="MKVIILAAGNAIRLASLANGSPKCLLPLGNTTILGKQLHNLEKV
                     GLTDVILSVGYKVEKVEEYLKNFRTNLKIHLEFNPFYDISNNIISLWNVRHLVWNQPI
                     IIINGDNVFDYQILSLLLESPFENVLMIQKKDEYDGDDMKVKAENDRLKAINKTMKSS
                     EANGESIGIMKFSAEGCALLMQKIEEMTRNKENLQVWYLRSIQELINDGLPVHVCRIN
                     GLKWEEVDFPEDYLRVKNMNWEGN"
     gene            47016..>47548
                     /locus_tag="PKX09_00930"
     CDS             47016..>47548
                     /locus_tag="PKX09_00930"
                     /inference="COORDINATES: protein motif:HMM:NF016852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HOU16427.1"
                     /translation="MPGKIKLDDKYLEKSLRLLERATTLLEKYNIPYWLEGGTLLGII
                     RENRLLPWDNDLDISIRSEDFDRLRRILPKFFYQGMIAKVRGHKIDDPPFQKGEVRLI
                     KVYATKYLFFKTPLVLDIFVKKKLDDQYYWVVGVKRRAKKAVPARFYDELTTVQFNNK
                     TYSIPKLTDEYLTYRYG"
     assembly_gap    47549..47648
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            47689..48426
                     /locus_tag="PKX09_00935"
     CDS             47689..48426
                     /locus_tag="PKX09_00935"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HOU16428.1"
                     /translation="MKAVILAAGAARRLAPMTNSIPKCLIKIGDKSILENQLDALQFY
                     GIEDVLIVVGYLKEQIIEKIGNQYKTINISYIENPLFSSTNTVYSLWLARAYFAGQDF
                     FYFNADVLFHYSLIGKLLEAPYESAMGVEVKQCGEEEVKVIVDDKLRIWRIGKKLNLA
                     DCLGEFVGVAKFGASLTADFSNALKAVIGEGLQMSFFEAAVDRILDQHALYSVDISKI
                     PVIEIDFPEDLERARQQIYPKIISFND"
     gene            48496..49584
                     /locus_tag="PKX09_00940"
     CDS             48496..49584
                     /locus_tag="PKX09_00940"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HOU16429.1"
                     /translation="MTTVLFHLHHLYYLPQFLPVAQAMERDGDFEVFYSTVIDKSRND
                     YEWSRKIIGDLGGNFITVDSEKERVTKIIKQNFDVVVFGKSRGAEKFCQPSSLAVLLY
                     HGIGVKACYYTDYNPRIDVRYVESQYRLEELRRRNIKTELVVTGFPKLDPLIDSSVRD
                     YYFDKYHLAKSKPTILYAPTFYPSSIEVFGESLAEQTKDFNLIVKLHHFSWLMKKYQH
                     QRKLWQTLVQKYGHVRLAPVEEYNIVPLFSCSDILVTEASSTAFEYLATSRPIIIADF
                     VKLRWKHRLFRKNFERQRLDSAIIDELDFAYHLEQKQDLYEVLNRAINDVEGKRHLLN
                     RKRDQFLGNVDGKASERVVADLKNRLNK"
     gene            49587..50621
                     /locus_tag="PKX09_00945"
     CDS             49587..50621
                     /locus_tag="PKX09_00945"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HOU16430.1"
                     /translation="MRYLFFITKPYSISVIRPLYEEIFRGAWGEALIFATPPVKALID
                     FAAPSTDSIQAAIDFQPEVVFVPGNFVHDKIPGLKVQIFHGLCEEKGGHYKITGFFDL
                     YCTSGPLITEKFLQLSQKYGFFKVKETGWPKVDGLLKPYNRNEICQNLRIDPQSKIIL
                     YAPTFSPKFKSSNEILKVLNDIPRTGEQWIIKFHDLMASEDRRCFEKLPRDKFRIYTG
                     YDNTPLLQVADVLISDTSSIVYEFMLLDKPVVTIGAKVRQEKALNIESASDLRAAVER
                     SLKNPQEFAENRRRTLAQIHPYIDTLNSQRVLQAVEGFLSGITPGELKKKPLNLYRKY
                     KVRRQLGGWF"
     gene            50623..51426
                     /locus_tag="PKX09_00950"
     CDS             50623..51426
                     /locus_tag="PKX09_00950"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOU16431.1"
                     /translation="MCYRALHGEELTPEQLKHLCDRNWIRNNARHIYKDKVNILAEIE
                     VEINGVPQAVVIKLFGWRNRISCWLSPFMRSRAKKSWDASFSLLKCGVKVPKPLTVYT
                     QRRFGFIKCNFLLTEKIGNFTLARNWLRDPSAKPSDKRIIVETLAEMVRKMHQAGMVH
                     SDLTPGNFLINNTNPSEIYLIDLNRLKHKIFLTRSLKMYDIAKLNLCQCGFAEEHSDC
                     LWILFLEKYDKEEFEKNKLALRLAMRRLKNHRAMKSLRKSSKPKNLPTP"
     gene            51423..52166
                     /locus_tag="PKX09_00955"
     CDS             51423..52166
                     /locus_tag="PKX09_00955"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOU16432.1"
                     /translation="MKIRFSKKLIFIGWIGLTNLLFAQNRIISRFPIGEELTYSAWLN
                     FMKVGYSTITIKAIELVDGFPVYHVESITKTSPFYSRIYRVNDRFNSWIDLDGLFSRR
                     YEKSLREGKYKKDYQAYFDYNEKKAYTSRDTLDIDGRMHDALSVIFWVRAEYLFPGRV
                     IRLNNFDNNKLRLFNVIVSGLETLQVPAGEFQCFVLEPYADDGKLFKNQNKMTIYLSN
                     DSLRLPVMIDNEANFGRMIFKLENRTIRQ"
     assembly_gap    52579..52678
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(52680..52752)
                     /locus_tag="PKX09_00960"
     tRNA            complement(52680..52752)
                     /locus_tag="PKX09_00960"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52717..52719),aa:Met,seq:cat)
     gene            52863..54278
                     /locus_tag="PKX09_00965"
     CDS             52863..54278
                     /locus_tag="PKX09_00965"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: protein motif:HMM:NF006055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HOU16433.1"
                     /translation="MKETLLIQNPLMCAPMTGERSSMKNIKTFSGGHIFVENGKIKSI
                     GPQEFNGKADRVIDASRMVVLPGLINTHHHFFQTLTRNIFPTENAPLFEWLKTNYTIW
                     DGITESAIEISAEVAIAELLKTGCTTTSDHLYLFPAQADANLLDMTINVAREMGIRFQ
                     PTRGSMSLGQSRGGLPPDNIIQKESEIVADIRRLIKLYHDDSSGAMTRIALAPCSPFS
                     VTIELMQMVVKLADEFNLQIHTHLAETLDEEKFCIENYGLRPFALLKNLGWIRPNAWF
                     AHSIYLNESEIAEMGVVGCGVSHCPTSNMRLGSGIAQIREMLDAGVNVSLGVDGSASN
                     DSSNMLLELRNAMLVSRLRQKGEWLTTDEILWMATRGGAAVLGRDDIGQLAEGKCADL
                     FLVSIDRIELAGAQHDLLAALIFAVLLSPVDYVIVNGRLIIEESQLMTADEQNLIKCH
                     QRISDELIMRAERKTGKKFRR"
     gene            54321..55928
                     /locus_tag="PKX09_00970"
     CDS             54321..55928
                     /locus_tag="PKX09_00970"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOU16434.1"
                     /translation="MKQSVVRKILVIVVWISFIPLVGIAREKADWEGGYPDGCTSVMV
                     GKKASRDGSVMTSHTCDSHRTRSSLDIVPTKKYSKGEMVTMLKRIDCDSLAMPAYQYL
                     AIGEIPQVVSTYGFINTAYPCMNEKQLAIGESTFGGRECLRSDNGLIDCQQLVRLMVE
                     RCSTAREAIRMAGELTKKYGWIDEGECLTIADKNEVWALEIVGPGKGNCGSIWVAQRV
                     PDDHVSVNANGSRIRQIDLKKPDYFMASENVFKVAQDSGWWNPKESPFEFAYAYAPES
                     RQTFSARRREWRVLDLVAPSLKLHPDAENFPFSVKPDTLVDLAKMIRIFQDYFEGTDY
                     NFVKNITWTNSEGKTEISPFANPFMPYDMNHIFKINGGWGWRGERPIARWYTMYATIT
                     QSREWLPDEIGGVVWLALDNVATSIYIPLYCSIYDVAPPYKVDGRTKGFNRESAWWAF
                     NRLGTLTAQRWGDMRYDVAAVWGPMQSELLRRQNEIERQALGQYRHSPDAARHFLTKY
                     SIDWGAKVVNRAWKLGDELWTKYDEQF"
     gene            complement(56180..57157)
                     /locus_tag="PKX09_00975"
     CDS             complement(56180..57157)
                     /locus_tag="PKX09_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU16435.1"
                     /translation="MIDVFFYEAFQEEAELIERFLPENITAGFDWRTIQESEHPAPPA
                     KIISIRTQSIVPVSWLPELSAILSRSTGYDHLIPLAQKAGSRLQVGYLPLYCNRSVAE
                     QAILIALALLRKLPQQLQQFQKFHRDGLTGRECAGKTLLVVGVGNIGYEVVKIGIGLG
                     MQVLGVDIVKKHSDVNYISIEEGLPKADIIICAMNLTSENHNYFNADRLRKAKPGVVF
                     VNIARGEQSPSTELLKLMQEGHLGGVGLDVYNVESELAVALRSGSTSPNKEVQATLAL
                     AKLPNVILTPHNAFNTYESVERKAAQSITQIQHLLENGRFLWPIPADGK"
     gene            complement(57218..58297)
                     /locus_tag="PKX09_00980"
     CDS             complement(57218..58297)
                     /locus_tag="PKX09_00980"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOU16436.1"
                     /translation="MPVNQIQLMRFKSDEPVDQTKNRLKKLFKSAGLDRVIQAGDITA
                     IKIHFGEKGNVTHVPAAYVEPIVRMIKSCGARTFLTDTTVLYKSVRQNAIDHLQLAYD
                     NGFTYEKVGAPVIIADGIIGRNETEIEVSAPLNKTVSLATELVAANSIIAITHVTGHL
                     STGLGATIKNLGMGMSSRRGKLAQHSVSKPIIKSNKCTACGLCMKWCPANAIEIPDKF
                     AIIDQNICIGCGECLTVCRFDAVHFKWNNSSTRLQQQIAEHALGIVRQKNGRIGYLTF
                     LTNMTKDCDCLPGKQVPIAPDIGVLAGFDPVAIDQAVIDLTNQVNDQNIAQLAHPEID
                     YSIQLDYGEKIGLGCRKYQLIETEF"
     gene            58505..>58785
                     /locus_tag="PKX09_00985"
     CDS             58505..>58785
                     /locus_tag="PKX09_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOU16437.1"
                     /translation="MNSGLFIPKNYRTILTTVETEKAILALKDFFQQNLAFELDLIRV
                     TAPLFVKAGTGINDDLNGVEQPVSFTVKEMANTQVEIVQSLAKWKRMMLT"
     assembly_gap    58786..58885
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <58886..59323
                     /locus_tag="PKX09_00990"
     CDS             <58886..59323
                     /locus_tag="PKX09_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018919472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOU16438.1"
                     /translation="CKKFGVIFLIGIGGQLQNGEPHDGRAPDYDDWSTPTVNGYHGLN
                     GDIIVYNRVLDCAFELSSMGIRVDREALLRQLKIRQVEERQNLYFHQRLLKGELPLSI
                     GGGIGQSRLCMFYLRKAHIGEIQCSIWPEEMVKLCQRNNIFLL"
     gene            59491..60180
                     /locus_tag="PKX09_00995"
     CDS             59491..60180
                     /locus_tag="PKX09_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16439.1"
                     /translation="MNTISKYKVGLILLGLVLTIPLSAAKKINFTEAKARYSGLMIFQ
                     TNEGSGIGGYFEYSLKNANRLAAQVMFTVATGDDYPLYYYDYYYDQYYYYESASKRRL
                     SFLHLLGGYKRILFADKIANDFRPYLECLGGAVMALDPPNIPDFSDRIKKMTTAWAPG
                     FQIGGGIDFLSGPKTLVSIFAGYDYLKFAQKIDPPQVYYDEDGNPLENRYSGQKDFSG
                     LVIKISFGKKL"
     gene            60180..60917
                     /locus_tag="PKX09_01000"
     CDS             60180..60917
                     /locus_tag="PKX09_01000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOU16440.1"
                     /translation="MHAYQFYISMEKIQEGTFDLEGDEYRHCCRVLRKIRGDSIDVFA
                     GNGRRWSARIEKISNNKAECRIMEEYPPQSRLKPEIILGVGLLKNNLMDDVVVSATAL
                     GVAGIMPLYTTHSIKNKINHERLAKLALSALKQSGLAFLPIISDCLTLDKWFNLTEEI
                     PLKLIAEQHASADLNRIQQLQTVEQVALMIGPEGGFEENEIENAQRFGFNKVNIFPYR
                     LRTELAVVTALAGIRTLTLKNEAYNGN"
     gene            60907..61254
                     /locus_tag="PKX09_01005"
     CDS             60907..61254
                     /locus_tag="PKX09_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018194996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOU16441.1"
                     /translation="METDCIFCKIAAGKLGTEFLYESDEIVAFRDIHPKAPTHILIIP
                     RRHIPRITDLQESDEKLVGRMVTVANILAEKEGLKESGFRLIFNCGRDSGQEVFHIHL
                     HLLGGRPFHWPPG"
     gene            61267..>62526
                     /locus_tag="PKX09_01010"
     CDS             61267..>62526
                     /locus_tag="PKX09_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU16442.1"
                     /translation="MYISKLTLYGFKSFLKKSEIQFGRGITSIVGPNGCGKTNIVDAI
                     RWVIGEQKASILRAERVTDVIFNGTATRRPLNLAEVSLTINNVSGRVPIAYSDVEITR
                     RLYRNGESEYFINRNLCRLKDITDLFIDTGMGANAYSIIELKMIENILSETPEERKRL
                     FEEAAGVNKYRVQRKAALRKLESTRDDLLRLNDIINEVDQNVKNLKRQIARYDKYQET
                     TQKLIEAEVILASRRIFDLRAKQAPILTDINAKQNLLEKISAELNAKESQGREQQKIL
                     NTIEIELQNKRNELETLRANKAKLEAAGLLLKEQLRNNTQNSARLTATINALQKSIES
                     AETRQKIIEQDLATNAQTLEEKRQVYKAAVEAQQEIENHYKKLSADLQALQDERFNAF
                     RQQAEQTAHLNNLKENINQREKELQKVI"
     assembly_gap    62527..62626
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <62627..64141
                     /locus_tag="PKX09_01015"
     CDS             <62627..64141
                     /locus_tag="PKX09_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16443.1"
                     /translation="GRQARLNELQEEIKSVEKQRTALLEQLQTAQAEIRRHSEMREKL
                     YRDRQQIETEIRQLTDVLRATESQQVQSAGALAALNDQKVMLTTAIANFKERLAKETP
                     TQTNVDSQLAQMDQLISIKKTELDQVRANLDGHNQNLQNLRVELIGLENKARNLSDEK
                     VALERNLKNSLEQVEKAQQELAALELEKSQLEKNLAENKLADAEAVTLVNQTESNYNQ
                     IGQEFQALRQTIEAINTEIINLRHTKEQNSEELKRLELASSQFIANINEISSVLWEKY
                     NRTPLEEIPPDLPTETEARQVVERYRHNLEQIGMVNMAVKEEYEQEYTRWKFLSDQRD
                     DLVSSEKGLTEVITQIDQVAREQYLETFNQIRSNFKTTFDIFFGGGEADLRLIGSDDP
                     LEAEIDIWACPGGKKMRSLRMLSAGEKALTAIALLFAIYQVKPSPFCILDEVDAPLDD
                     ENTRRFTSVIRTFSENTQFIIVTHNKATMAIADALYGVTMAEKGVSQIVSVKLE"
     gene            64145..65515
                     /locus_tag="PKX09_01020"
     CDS             64145..65515
                     /locus_tag="PKX09_01020"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOU16444.1"
                     /translation="MRIKIFLMLMCFLIVNSFAVEFDVDYAVFKGNEEVSVVEVYLLI
                     PRTLFQFVPNENEYCSYALIRVALVEGDSVRDIQEWQITDRVADTLQLAENQKIPEIA
                     TLQVKPGNYQLIIVVADLINRHQYHYDKQIEISDYSDDQLRLSDIQISSQISKTSQEN
                     KFSKYFGYDVIPNASAIFSQRQNLIYSFVEVYNLKVDPGKPSNYLVKYDIADLNGKVV
                     VSSDWRSRKKPGTSAVEINNLSIAGLPGGLYDLIIEVNDEATQKTVKKIKRFYILESK
                     SQLASLEMNAEAIQLEGKSEKELDEIFGPLRYIATETEIRRYKKSDVAGKKQIILHFW
                     DNRDPNPQTPINENKAEFEQRLQYVNQQFSTNQVPGWKSDFGRVFMIYGMPSEIERFP
                     SALESKPYQIWYYNEIEGGVKFYFVDKNGFGTYELVHSTARNELQDINWQRWISPYST
                     SPDLTY"
     gene            65532..66395
                     /locus_tag="PKX09_01025"
     CDS             65532..66395
                     /locus_tag="PKX09_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU16445.1"
                     /translation="MRIGIDLGGTKVSIGLVDQNRLIGQPVRFMIAECKDADDLVKHM
                     KDAIDRSLKANGIVWQNLEMIGIGSPGPLDYKTGIILHTPNLVMLRNYPLGPKLEALT
                     GLPVLVNNDANCFALGEQRAGGGRGLEYVLGVTLGTGFGLGFIYRSEIFNGATGTALE
                     FAMTPYLDGVYEDYISGRGLARIYKSVVGKEIKPAEVTALAKKGDSDALETWRIFGHH
                     LAQAMIVLVMVLDPDIIVIGGSVSQGWDFFYQTLETELRAGIHAGPAGHLKITRSELG
                     ELAAIIGAASL"
     gene            66495..67094
                     /locus_tag="PKX09_01030"
     CDS             66495..67094
                     /locus_tag="PKX09_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOU16446.1"
                     /translation="MDSNVGLICADCWQKLQLFPAEAIKNKIIPESLDQVWPVFLFDE
                     LFQKIIHALKYQGNVSIGYEIGRRMALHLPSEIKDLQSACYVPIPLHPIKLRERGYNQ
                     SEAITRGLASNSGIRVETKLLKRIKNTATQTKLNAEERQANMEMAFAINQKSSVSSGN
                     TLILIDDVFTTGATLNSAAGVLRQSGFHKVIAVTAAAPL"
     gene            67183..68379
                     /locus_tag="PKX09_01035"
     CDS             67183..68379
                     /locus_tag="PKX09_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOU16447.1"
                     /translation="MSRLTVDDLKVKGKRVIMRVDFNVPLDENLNVRDDFRIRAALPT
                     IQKLLNDGARLILMSHLGRPKGKPVPELSLKPVAECLQKYLKNKVYFAPDCVGIEVKN
                     LANSLQDGEVLLLENLRFHPEEEKNDPEFSAKLAELGEIYVNDAFGTSHRAHASTAGI
                     TNYIPVRAAGYLLEKELHYLQDMLENPERPFTAIFGGAKVSDKIDIIERLFGKVDNIL
                     IGGGMAFTFLKAKNYEVGKSLLEADKIDFALATLKKAEQMGCKIVLPTDVVVASEISD
                     TAVPEIVSVDQIPPDKIGVDIGPETTMIFCEIIEKSKTVLWNGPMGVFEVKQFSTGTE
                     SIARKLAEVTTKGVITVVGGGDSASAMKKFGLMDKVSHVSTGGGASLELLSGKELIPI
                     NLISKK"
     gene            68386..69165
                     /gene="tpiA"
                     /locus_tag="PKX09_01040"
     CDS             68386..69165
                     /gene="tpiA"
                     /locus_tag="PKX09_01040"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOU16448.1"
                     /translation="MKQYIVAGNWKMHKDIQESRIFVSNILNKLLKHERATVILCPPF
                     TALFDVYNLIKNTSIALGGQNMHSEPKGAFTGEISGGMLISAGCQYVILGHSERRHIF
                     GETDDFIRDKVKQALQVGLKPIICVGEKLDERQSGETLEVVRRQCDSALSVVNEQEIK
                     RCVIAYEPVWAIGTGVVATPEQASESHSMIRQLLAAKYGQESAAGTSILYGGSVKSSN
                     AASLIQAQDIDGFLVGGASLIEDEFARIAEIVDEYVKHKES"
     gene            69165..69512
                     /gene="secG"
                     /locus_tag="PKX09_01045"
     CDS             69165..69512
                     /gene="secG"
                     /locus_tag="PKX09_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOU16449.1"
                     /translation="MYTFLIIIHVIVSALLMLVILMQSSKGGGLSGTFGGAGGSSLFG
                     GHGAATFLSKLTTGLAIAFMVISLLIGLISAPRGEPTSIIRQEANRRTSPSAELPIPQ
                     GSSEQGTPVLPGN"
     gene            69560..69645
                     /locus_tag="PKX09_01050"
     tRNA            69560..69645
                     /locus_tag="PKX09_01050"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69594..69596,aa:Leu,seq:gag)
     gene            69678..70967
                     /locus_tag="PKX09_01055"
     CDS             69678..70967
                     /locus_tag="PKX09_01055"
                     /inference="COORDINATES: protein motif:HMM:NF020855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU16450.1"
                     /translation="MKYVKQISVIILITLVVLSSLIGQDPLSEAYTLFQNGEYQKAFE
                     KCKPIVEKDPNYAGAVFLLGRIYFALGELDSSKVYLDKAIDLDRANTEFREIRTQIAA
                     FTTQLTEASRLVNNGDYEGAKNLYLEIIKANPNFADAYFNLAQVYIRLNDLTNASLYL
                     RKVIEMKPNEERYTKLLKNLVQQYLQEGENLRQRRNNEGALEKYKQALELDPNEFLAY
                     YFSGLAYYDMKNYDEAIKAINKGLEINPEHAKSYLLLGQIYEKTNKKAEALKAYEQAV
                     KIDPLFEAGWDRIGVLNYNMKNYEQSISAYKKLIEINPENPRYYEKLGQIYLDTNNPK
                     EAIINLEKVTQKDPKNLKAWLRIAQAYNNQGECAQAKTAAETALKIKAGDALALIELG
                     IAERCLGNTVAARQAFQLAAKDPQYRRYVEEHLKTVQ"
     gene            71119..72675
                     /locus_tag="PKX09_01060"
     CDS             71119..72675
                     /locus_tag="PKX09_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOU16451.1"
                     /translation="MPEFILSNAEAAAVDRYAIEELQIPGTELMFKAGNFVALKAKKL
                     IQDIPNSRIDLFCGIGNNGGDGFVAALLLSEWGANCIIWLIGDPQNIKGDARHFYQKW
                     VESKQTIRCIKTAEDFEEYSDLPESDLIIDAMLGTGFHGEVQGIIADVIRRINNINLP
                     VIAVDIPSGVNGDTGEVRGDAVKADYTVTMGFLKRGLLFYPGKRFAGEIVVVDLGYPR
                     KAFDILKEKTFLIGKQDVKKVIPPIAEDTYKHRQGKVLIIAGSPGMTGAATLCALGAM
                     RSGAGLVVNAIPKSLNPIMEVKLTEALTAPLPESSQQTFCLESLTALQSRLEWSDVVV
                     FGPGVSDNVEVQEFGQQLITKCHKPLVIDADGLRIFHNNLELIGTIEDLTITPHRGEF
                     AMMTGIAVAEVNKNVIEVARDFVRKYPCTLVLKGAPSVVVSSTGVVAVNSTGNPALAT
                     GGTGDVLTGIIAAFRAQGLNSFDAAMAGTSIHGLAGDWGRKKLGVRAFIASDLLDYLP
                     LILRRLDRVI"
     gene            72675..73055
                     /locus_tag="PKX09_01065"
     CDS             72675..73055
                     /locus_tag="PKX09_01065"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOU16452.1"
                     /translation="MNRFWLKVPAILYAALIFSLSSLTKTPLVEIPIWNVDKLVHMLE
                     YTIFGILLVLAFRSDQIGKPMRRANFQSIILGILYGLTDEIHQYFVPGRTSSLADLGA
                     DALGILLGVWIFNRLNVFQKFREA"
     gene            73052..74752
                     /gene="recN"
                     /locus_tag="PKX09_01070"
     CDS             73052..74752
                     /gene="recN"
                     /locus_tag="PKX09_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOU16453.1"
                     /translation="MSLTKLKIDNFAIIDHIEVAFGPGLTILTGETGAGKSIIIDALN
                     LALGEKASPNVIRAGADVATVECTFDLTDLNPATKNILKSHNFYQENNPLTLKREVNI
                     NGRSRAWLNGETCLVSVLKEVGNLLVDMHGQHDHQSLLNPDTHIEFLDAFGDYAEWRE
                     QVARQFDDLKLLFERQNLLQEQLRLNREKRELWEFQLQEIQKVAPQENEYETLLQEKT
                     ILENTAKIQQLSSDLTYQLLESGDSLYNQLQSVVKQLSNLQKITGSFAEQLSRLEESQ
                     YLFQEIARQIAHFGDDLQFNPARLEIINQRLYSFQQLMKKYGRSLSDVLAYRQKLETD
                     LTESDDLEVQIDKNRELIKEHLEKFTRFAFELSEKRKQSAELFKIEIEKILERLGISG
                     ADFAVRLELMPDQNGWIIIDGQKYKAESTGIDKVTFEISTNRGEPRRPLAEIVSGGEV
                     SRIMLAIKTILAGRDHIPVLIFDEIDTGISGKIARKVGEELHELAKVHQVICITHLPQ
                     IAGLADEHFSVEKVTTNSRSQTQIRKLLPEERVAEIAKLIGGRSVSEITLKQARELME
                     "
     gene            74755..>75224
                     /locus_tag="PKX09_01075"
     CDS             74755..>75224
                     /locus_tag="PKX09_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU16454.1"
                     /translation="MDFSELILKRRSVRKYLPKPVERELIDKCLDAARLAPSACNAQP
                     WYFIVIDNEEVKDRLCNAAFSGIYSSNKFVKNAPVIIVVITDQKSYQVKVGGFFRHLK
                     YALVDIGIAGEHLALQAAELGLGTCWLGWFSENGVRKALDLPKSTKIDIMFSLG"
BASE COUNT        22082 a        14394 c        17150 g        21098 t
ORIGIN      
        1 ccgaataaat caaaaactct gagaaaaaac caactacact cagttgaaga accgctaaac
       61 ggagagaata acgcttcttc ttaagcccta ttagaaatag caatttgata ttaagaatcg
      121 taattcagta tatcaagtaa aatatttcgt aggacagcct ttccaggctg tcagtgatcg
      181 acaggcagga atgtcttttt gttgtagggc gatttgttaa atcgcccagt cgagttacca
      241 actcgactta cgatttatag acaggcaaga atgcctgtcc tacggttgcg gtcaaaaatc
      301 aacaggcagg aatgcctgtc ctacggggta tttttttcag gcgctgattt tttatcatgc
      361 caaatttgtg ttaatttaca ttgctgtttt tataaagaat tatgatatag ttatagagga
      421 taaatatatg gcgcgcaaaa tcgtgcatat aattggtacg ggaacagttg gcgccccgtt
      481 aatcggcgtg ttactcggtc accttgaaca attgggtatc gaagaaatct cctttgaacc
      541 aaatttaaaa gccatagaaa ataaagcgtt cctgaatgga ttggtggctc gcggcgcgaa
      601 attatgcgtt cgcgaggaaa ttgccgatga atttataagc gtcggatgca aaattgatta
      661 cacatcgaaa gaggcaattg aacgggcggg agtgattata gattgttcgc ctggtgatga
      721 gccattgaaa aataaagccg aaatttattc gcgcctcgat ggtgagccaa aatttttcat
      781 cgctcaatcc agagcggccg gttttggttt agattatgcc tacggcatca acgatgaggc
      841 tttggtatcg agcgaagttc agtttgtccg ggttgtttcc tgcaatgcgc ataatattgc
      901 cgctatgatc aaaactttgg ctttcgatga aaaaggcgaa aattacttag attgggggcg
      961 ctttgtgtgt attcgccggg cgagtgacat tagtgagaat aaacctttta ttcctgcacc
     1021 gcaaattagc ctgccccagg atgacaacta tggaactttt caagctttgg atacggcttc
     1081 tctgtttcgc actataggag ttaatctcga tattttttca tcggcactga aagtcaattc
     1141 tcaatatctg catattgtca atttcgatct aaagctgaaa caaccggtta ctttgccttt
     1201 agtgatcaac aaacttgaaa gtaattcgct catcgctctg acaaacaaga cccagtccag
     1261 tctggtattc gccttcgcgc gtgatatggg attcctcggt cggatcctca atcaatcggt
     1321 ggtttccgtg ccgagtctta ccgttaaagg ggatcgggaa ataataggtt tctgcttttc
     1381 gccccaagat ggaaattcta ttctcagttc gattgcggct actgtctggt acttttatcc
     1441 gcattcttac cgggacaaat tgcaaaatct ggatgatcta atttttgccg aagtctgaat
     1501 ttgttaaccg cagaggatgc taagaacgcc gagtaattat tgaacaaacg taataatagt
     1561 tttgaattaa ataaggtcaa attatacacc ttaagtattt tgctttacgt atgattatat
     1621 gactcgggtt tcgtttttta aatttcgaat aaactcaata tgctgataga acaaatcatt
     1681 actcaacaat tttcagaaaa tgtctggctg gtaggtgacc cgcaaacgcg gaaaggattg
     1741 attattgatc ctggtgacca cgccagtgaa attatcagta aaattagcgc gatgaacatt
     1801 caaccgctgg caattttgaa cacgcatgcg catcctgatc atttagccgc cgcggctgaa
     1861 gtgcaaaatc attacaatat accgttttat cttcatgcta aagaaaaagc cgttttgaaa
     1921 tccgccattc cttttggatt ggtgctggga ataagaatta ctcgagtccc cagtcaggtg
     1981 atttatctac aagatgaaga aacacttaaa atcgaacctt ttgagattag agtgattgca
     2041 acgcccggtc atactcccgg aagtgtttgt tatctgatcg ccgatcggtt attttccggt
     2101 gatacgctgt tccggaattc ggtcggtcgc gttgatttcc cgggcagttc ctggcaggaa
     2161 ttgaagcgca gtttggagaa attaaagaat ttaccgccgg caaccgaagt ctatcccggt
     2221 catggtatga acactactat tgggcaggaa ttatctcata atccgtatct aaaaaatggc
     2281 gaatggcgat gaaacctacc actcttcagc ggaagtttgt agaatacgtc gagcaaaacc
     2341 atcttttaaa tgatgtgcga accgtagtcg ccgccgtttc gggtggagtg gattctatgt
     2401 gcctgctgga tttgttaatg tcagagaaaa agcgctggga catcgagatt gtcgttgctc
     2461 attttaatca tcagattcgc ggtcctgagg cggattctga cgaaaacctt gtccggcaat
     2521 tttgcgataa tcactcattg cctttctttt ccgcagcgga aaatgtgccg aaaatcgctc
     2581 acgctaaacg gatctctctc gaaatggctg gacgggaatt gcgctatgct ttttttcaga
     2641 aaattgcggc tgattttccg aactcaatca tcgccacggc gcatactctc gatgatcagg
     2701 ctgagacgat tctgctaaga atgctaaaag gcaccggttt gcaaggctta tctggaatta
     2761 cgatgcggcg cgagaatatt agacggccgc tattgtttgc taccaaagcg gaattgtatg
     2821 actatgcccg cgcgagagaa ttgccatttg ccgaagacca cactaattta tgtaacgact
     2881 acgaacgtaa ttttattcgt aatcagattc tgccgacagt acaacatact ctaaatccta
     2941 atattagagc gactttagct aatttggcgc aaaatttagg cgaagcgcat caatttattg
     3001 tcgaatttgc cgaaaaggcg ctggagagtt taattattaa ggcgaattcc gaacaaatag
     3061 tgcttgataa atctagcctt aaaacctatt ttatcgcagt tgaaaaagag ataatctggc
     3121 aatctttaca aaggttatat ggcacaatta agccattaaa ttttaagcag atgaaccaat
     3181 tgattaaatt agtgaatgaa ggtgaaaccg gacggcgtat gaatcttggc ggtgaaatta
     3241 cagttctaat cgatcgagag cggctgattt taaagcgcaa tcggtcgtcg aattggtcat
     3301 cttcacccat tatccccggt gagaaaatta agaatgagtt tttcaccttt tccagtgaac
     3361 tgatggatgt aagtaatttc aagaagggga aacggcgctc aggagtagag tttattgact
     3421 ttgatctggt tggaaataat cttggtttgc gacattggca gagaggcgat cgcatgatac
     3481 cactcgggat gaaccaatcc aagaaaatca gcgatatttt ggtggatttg aaagttcccc
     3541 tcactgataa aaacagcatt ccagtctttt atagtgaata taaaatcatc tggctctgcg
     3601 gcttaacaat gtcggatgaa tttaaagtta cctcgaaaac taaaaccgtt ctgaaattga
     3661 tctacaaggg aaaaaagata tgaaaaagac gataactctg gatgccagtt atggcccgta
     3721 tgccggccag gtaatcgaag aacttctgac ggtcgcccaa atcaaccggc gagttacgga
     3781 attagctgct gaaatctctg cgatgtacca aggaaccgta ccaattttta tcggggtatt
     3841 aaacggcagt ttcatctttt ttgccgatct attccggaaa ttaacaattg acggtgaagt
     3901 agattttatc aaaatttctt cctatgccga tcagactcga tcttccggaa cgattttact
     3961 gcggaaggat attagctgtg atataaccgg tcgagatgta ataattatcg aggatataat
     4021 tgattcagga ttatcaatca aatttctaaa acgtcgtatg gaagaaagta atccgtgctc
     4081 ggttcgcttc gcggcgttgt tgatgaaaga agacgcgcgg gtggatttcc cgattgattt
     4141 tgtaggattt aagataccaa ataaatttgt tgtcggatat ggtctggacg ttgcccagag
     4201 actacgcaac ttagcgtcag tttatgcttt taagtaagaa aaaaaggaat tttaactata
     4261 tgtcagaaaa taactacccc aaaaaccaaa gttcaaaaaa tttaaatgaa ccgaggaagc
     4321 cccgtcagcc gaggcgaaga cctccagaac cgcctaaacc aaataaagac gatttccgct
     4381 ggaaaaaggc ttcacgcact tccctgatgt ggattatcat aattctcagt tcgatcgttc
     4441 tattacaaat tctcggacga ggcgcccaag aagaagtggt cattacctat tcgcaataca
     4501 aggaattgct ggatcaaaaa ttgatcaaat cggcagtgat tatcgagaat gaatttcatg
     4561 ggacgttgac tcaaaaagtt cccttagtag ttggtggcag gacgattgaa gccgaaaaaa
     4621 ttttagtcaa tttacgtttt gttgatagtg agatgctaaa aagctgggat gaagccggga
     4681 tcgaatacga ttttaaggta aagaatacca agtggacgaa ttttttcatc aatattttcc
     4741 ctttagtctt cattattcta atgactctat tttttctgcg tcgaatgcaa gggcaaaata
     4801 acaagggcat tttcggtttt gggaaaagta aagctcgcct ctggaccagc gaccggccta
     4861 aagtcagttt caacgatgtg gcgggatgtg ccgaagccaa ggaggaattg caggaaataa
     4921 tcgaattcct gaaattgccg tcgaaattct cgcgtctggg cggcaaaatt cccaaaggtg
     4981 cgttattact cggtcctccc gggacgggaa aaactcttct ggctaaagcg gtagccggtg
     5041 aagccggagt tccgtttttt agtattagtg gagctgattt cgtggaaatg tttgtgggcg
     5101 tcggtgcctc ccgagtgcgc gacctttttg aacagggtaa acatagtgcg ccatgtataa
     5161 tttttattga tgaaattgat gccgttggac gacaacgtgg tgccggatta ggtggcggtc
     5221 acgatgaacg cgaacagaca ttgaaccaat tgcttgtcga gatggatggt ttcgatgaga
     5281 ataccaatgt cattatttta gcggcgacta accggcccga tgttttggat accgccttgt
     5341 tgcggccggg gcgcttcgat cggcagattg tcgtggatat gcccgactta gttggacgcg
     5401 aagggatttt gaaagtgcat actaagaaaa ttcccctagc ggatgatgtt gatttgtcgg
     5461 ttttggcgag gggaacgccc ggtctggtgg gcgctgatct cgccaatctc gttaatgaag
     5521 ccgcgctttt agccgcccgt aaaaataaaa aagccgttga gatgattgac ttcgaagagg
     5581 ctaaagacaa agtaatgatg ggcgttgaac ggagaagtct acatatttca gatcgggaac
     5641 gccggctaac cgcctatcat gaatccgggc atgttttagt tgccgtcaat attccggaag
     5701 ccgacccgat ccacaaagta accattgtgc cgcgtggtcg ggcgctggga atcactcacc
     5761 aattgccgat ggacgagcgg cataattatt cacgctcata tatcaatagc cgactcagta
     5821 ttctgctcgg cggacgcgcc gcagagaaaa ttatcttcaa tgaattgaca actggagccg
     5881 gaaatgatat tgaaaccgcg acggaactgg cacgcaaaat ggtttgtgaa tggggaatga
     5941 gcgacaaact cggtccgctt actttcggta agaaaaatga agaaatcttc ctcggtcggg
     6001 aaattgctac tcatcgcgat ttcagcgaag aaaccgctcg tctgattgat caagaaattc
     6061 gtgaaatagt cgaaaatgct gagaagcaag cttgtgaaat catcaaaaag aatattgatt
     6121 tactacacaa attagcctta gctttactcg aaaaagaagt tttagatggc gctgaaattg
     6181 ctaaaatcct gggtaattcc gaagttaatt ctgctaatat attcgtaaat aaaagtgatg
     6241 ataatatgac taagccagcc actaaagcaa agaccggttc acgacgaaga aaaagcgaaa
     6301 aagaaaaagg agccaatact gatcaggcgg cttaaaaacg acgcgctcaa tcacctcgtc
     6361 actgaaatgg aatcagtgga tgtcgatccg gcgggaattg ctattatggt tagaaaaagc
     6421 gaattcctta tcctcaaaac cgacccgatc atcgcaccag ccgccaatat tctaaaacaa
     6481 caaatattgt ctgtcggcgg cgattgtgcc gtagcgcgtg aagtgcccac cggacaagca
     6541 ccaccaacgc cggtaatttt aatggcaact cgtcgtcaat atgaacgctt gataatcaat
     6601 ctcaaacagc aatgcttcgg acttgataaa ctcggcaccg aaattgaagg atttttagcc
     6661 cgaatttccg cgccaaggaa atttcatatc ggtcagcaag agtaccaaac cgaccagaaa
     6721 accttaatta tgggtatttt aaatgtaacg ccagattcgt tttatgacgg cggtcggtat
     6781 gcggaaatgt ccgccgctat agagaccgct ctaaagatgg aagccgacgg cgccgatctg
     6841 attgatattg gaggagaatc aacccgtccg ggttctgatc cgattccagc cgaggttgag
     6901 attgcccgac taaagccggt tttggagcag ttgcagatgc gccttaaaat tccgatttcg
     6961 attgatactt ataaatcggc cgtggctgag tggactttga ataacggcgc gctgataatc
     7021 aatgacatta gcgggcttaa ttttgattcc cagatggcgg gtacaatcgc caagcacaac
     7081 gccggggtta tcctgatgca catcaaagga actcccaaaa atatgcaagc tgatccgcat
     7141 tatactaatt tgattgatga aataatgaaa tacttatcgg attcggttca aaaggctcgc
     7201 actgccggta ttgacggctc gaaaatattt atagatccgg ggattggttt cgggaaaaca
     7261 gccgaggata attaccggat aatgcgttat ttggcagaat tccaatcact cggatttccc
     7321 attctggtcg ggccatcgcg gaaagccttt atcggacagg tactcaattt accggctgat
     7381 gagcgattgg agggaactct ggcggctgtg acagccgcag ttctaaatgg cgctgattta
     7441 gtgcgtgttc acgatgtaaa agccgccgtt cgcgctgtaa aaattgccga tgctataaag
     7501 ggaaaatcat aaatgactct gtttaccatc aaatttttaa gcgttaccat ctgggacgta
     7561 atagatataa tcgtcgttta tataataatc cttaaactct atcaattttt taaaggctca
     7621 cgtgcttcgc aaatgctaat cggcctggcg ataattttac taacatcttt tattgccaga
     7681 gcgctgaatt tgcagagtct atcttggata atgggtaatc tgcaaaccat ctgggcgatt
     7741 gcctttgtga ttatttttca gccggaactg cgccggattt taatttattt ggggcagagt
     7801 cgctttgtca gacgattttt aagtgaatcg aaatccaaaa cgattgaggc ggtgattgaa
     7861 gcatcccggg aattggtgcg gcgtaaatgg ggtggtctat ttgtgctggt tcgggaagtg
     7921 ggtttgaagc caataaaaga gcgggcaact cccatcaacg ctgaagtctc agccagcctt
     7981 ttagtttcaa tcttcaatcc cggatcacca ttacacgatg gggcggtttt aattcagaat
     8041 gacattattg aggcggccgg ttgtattctc ccgctttcgg atagccctga ccttgatccg
     8101 caaatgggta ctcgccaccg cgcggcgctg ggtattagtg aagaatcgga tgcggtggta
     8161 gtagttgtat cggaggaaac ccaaaaaatt gccgttgcct atcagggtat attatatcgt
     8221 aatgttgacg aggattcctt aagagcattg ttaagccggc tgttcttcag aggcagtaat
     8281 aatagctaga tgggcaacag gtaagtaatt atgtcgataa aacctgtccc attttatcta
     8341 tctcataata ttaaacaccc acttcggatt ttattctgcg gatttatttt actaatcagc
     8401 caaaatttat tgtggtcggc ggtttctgat attgaaattg tggaaaaatg gctgaacgct
     8461 acgacctggc aatggcaagc cgaattacca atgccaaaat ttccagtaga tgagaaaaat
     8521 caaacctgtg ccattgaaat ttccggtttg gaaaatttag cgcgtgacgg ctatccgtca
     8581 atccctgtct atcaaaaaat tattaatgct ttaccggatg agattgcttt tacagttcaa
     8641 actggtttgg aaaaagttgt gccgttaccg gcagtgatga gtatatcgca ggatattcct
     8701 attgccggag aaggaacgaa tcaggaaaag attattctcc ccaaaacaaa aaattttagc
     8761 gcttatccga tgcgtttagt agaaattgaa tttctcggtt atttgaaggg catcccactg
     8821 agcagtgtca aaatttttcc ctgtcaattg attgagcaag gcaagcgctt aaaatatttt
     8881 ggttcagtga cggttcaggt gcaagtcaaa ccagggaccc ggctccaaaa aagtgttgca
     8941 actgacgaac aagcacttct aaaagccctc gatattgcac ccggttcaat cagaaaaatt
     9001 gctaaacagt cgccgacttt agcgaaaccg gcggtgactt ttccggctgg taaagtttta
     9061 gctaaattga ttgttgatga ggatgggatt tatcgcgttt cttgtcgggc gctaagcgac
     9121 agcggcattg ctttgaaaaa agttgacccg cgtactttcc gcctatttaa tcagcaacat
     9181 gaagtaccaa tttacgttta tggcgaatct gacggaatct tccacaataa tgactatatc
     9241 gaattttttg gccagcgcaa tcgcaacagt gtggctgatt atgaatacga tccatttacc
     9301 gataaaaatg tttattggtt gacctggggt gaaaattatg gtctgcgtta cgccgaagaa
     9361 tcggttcagg tgaattctcc gattgatgag acggttgtac caattgacta tttatatact
     9421 gcccatatcg aagtcaatgc ggctttcgag cgccttgggc aagtggatgt tgatcagccg
     9481 actcatgtcc gcgaccactg gtttttcgat tcggggattt ttggtggtac agcaagggaa
     9541 tacgttttcc atctgtctta tcccaacccc aatcaaatta ataacttcaa tgctcaatta
     9601 agaatgcagg gacttaccta tggcgatgca ggcgatcatc aagccgatta ttttataaat
     9661 aatatcaaag ttgctagcgg tgcctggaat ggtcaaaagg catatactct cgaaaacagt
     9721 gctagccagg ttttgcataa taattttctg aaacatggcg agaatgttct gcgagttcaa
     9781 gtttccggtg ctgatccgac cgacaaatat agcaaagtag ccgtggatta tttaaaggta
     9841 aaatacttcc ggctatacaa agccgacgag gacgaactcg attttacccg accggaaagc
     9901 tcgattgacg gactttatac ttttacactt tccaatttca acacttcgga tatttcaatt
     9961 tataaagtag gaaagagcaa actccgcgat tttacgacgg aatacatcaa aagcactaac
    10021 tcctaccggg taatgcttca ggattatgtg cgagatgata cgaccctcta ttgggcggct
    10081 tgcggggagg cgctgaaatt tccgctttcg gtaaaattgg atacgataat cggtctaagt
    10141 tcgatccctt ccggtgctga agtggtcgtt attacccgtc aggaatgggt caataatctt
    10201 tccaaattga ctgattttta tgccagcatc ggcttgtcat ctcaggttgt tggaattgaa
    10261 gacatatata acgagtttaa tgctgggatt atcagtccct ttgcgatcaa aaaattcctg
    10321 cagtatgttt gttataactg gaatcctgtt ccggaatata ttttgctggt cggcgatgct
    10381 gatataaagg aagagcgctc catcccttct tatttttttc aatcttatag ctacggagcc
    10441 tgtgccagcg atttctggta tgctctaata gatgacgatg atgtgcctga ttacgctatt
    10501 ggtcgctggc catgttcaac cattgaagaa ttggaagcac taattgacaa gcgcctaaat
    10561 tataccaaac acaatctcat tggtccctgg aataatgaac tgctatttat tgctggacag
    10621 gaaaacgttt tcaaaaatca atcagaaaat atgatccagc gtcagattag caaagaatac
    10681 aatatcaatc gtatttatgt caatccggct tcggtcggtt cgcggttcta cggcggttcg
    10741 gatacgctcc tatatttatt caataacggg attactttag gtaattttat gggacatggc
    10801 ggcggtgccg tatggggtga tcgctcgctt ttcaattcgt ctcatattcc ggaactcgag
    10861 aatctggatc gtctgccgtt tctgaccagt atgacctgtt ttaccggtga tttcaccaat
    10921 gtgactggat taggtgaaat gctccttttg gctgaaaatg gcggagcaat cggtttgtgg
    10981 ggagcgtcca gtgttggctg gattaaaaat gattatctat tggcgaaacc tttctacgat
    11041 gtcatctttg aacccggaat gacggtaggc aaggcgatcc agaccgcgaa aattcgctat
    11101 caggctgagg cttttgatta tttaagactt tcattgatat actcttataa tttgatcggc
    11161 gatccgacgg ttggtctgcc ttttccggaa caatctactc tgcttaacct tagccagacc
    11221 aatccggcac cgggcgatac ggttattctg cgtggcgact tgccttttga agcggggaaa
    11281 atgtatattc aacttttcga ttccagcaaa tatgcggtat atgagaaacc ggctgaggtg
    11341 ccatttagta gcagtaattt acaccatgaa ataattttgc ccactaatat taatcccggt
    11401 aatacctatg ttaattatta tatcgaaagc aagactgcca aacaagatgc tcacggagcg
    11461 acgctgtttg atattaaagg cttgaccttt tatggttttg attatgctcc atcttcacca
    11521 ggggaaaaaa cacctataac tttgtctatt tataccgaac tatcgaatgt ccagtcgttt
    11581 ttttgtgaga ttgatacggt cagcgcctat gaatatttgg acggagatgg cattgaacat
    11641 atcatcagtt ttcagaacga ttcagtttta ttatcaattc ggatgggtga ggttgaatcg
    11701 gtcgctaata aatggcaaac cacccaggtt ttccaagtag gcacgccggg gaaattagta
    11761 gccgtccgct ttgttgcaat tgactccagt ggtaatcgga cagtcagccc agcttattca
    11821 attaggataa aaagtttacc ggatttacgt ccgatcggga ttgcccaagg cggcgagcgt
    11881 tttccggaat tgattgctga ggtctcatat aatggtaatg acactcttag tgctgtcgtc
    11941 aaggcggagc gcagagttga tgagaaattt gagttgtttg gttcaaagga agtggtattt
    12001 ttaccgaatc gggcgacctc ggtaaaatta ccaggtatat tgggcaaaga tacagtcatt
    12061 tttagaatta cagtcgaccc ggaaaataca gtcgccgaat ccaatgaaat gaataatagt
    12121 ctgtcacaat ctatggtgat taatactttc gcggttctgc ccacctgtgg gacaacttat
    12181 gacggaagtc aatcggatac aattctggtg ggaagcgttt tcaaggtggc gatcgcacct
    12241 cttagcgttg ccgattcggc ggttctgatt ctgcagatag ataccaactt aaccatcgca
    12301 agtcagccga agtttagttt agtttcacct gattcgggat ttgaagcttt aggattgaaa
    12361 gctaccggag gaaactccac gagtacgagt ctattagctg tcaatgtgac cgttgatttg
    12421 agtaattgcc cggcggcgag tcaaattgat gatgtgggca ttggcaaatg gaatccttcc
    12481 ttgcgtattt ggtataatga agaaggacaa aaggaaggta tgaaaatcaa aacaacctcg
    12541 actattccgg gtacatttag tctaattacc aatactgaca cgaaaccgcc taatctggaa
    12601 ttaaatctgg agggtcagca atttttccag aattcgtacg tttccgaccg accgagtatt
    12661 tcgattatcg gcgaagatga taatggcgta cgctttgacg ccgaaggtat aaaagtgata
    12721 attgataata atgaagttgc ccttgagaat ttgcacgcac cggatacggt ttcgagcggg
    12781 cattattttt cggtgcaatt ccgtccgacg tttactcatg gtaatcattc gatggaggta
    12841 accttgaagg acgcggctgg taatagcaca accgaatcgg taggtttcat cgttgctgag
    12901 gaattaaaat tgattgatta tggcaattac cccaatccct ttaggaatcg cacgactttc
    12961 atttatgaac tgacgcagag agtaaattcc ttgaagataa aaatctatac cacttccggt
    13021 cgtctggtgc gggttctgga ttcggagaat gtatttgaat cgggtctgga tttaaacgaa
    13081 ggtggttatc atgaagttgt gtgggacgga ctcgatgccg acggaaattt cgttgccaat
    13141 ggaatctatt tctacaaaat ttccgccaaa tccggtaaca aaaccgtaac taaaaccggg
    13201 aagttagcca aagcgcgatg aaacgaattc tgataattgg gataatactg attggcttga
    13261 gtggcaggct ggtagctggc gaatacgccg atgcctttct cgaattggga gtaagtgcgc
    13321 gagcttcggc tatggctaat gccatcggag cgctggattt ttcggaaacg gcttttataa
    13381 ctaatccggc agggatcagt tacatccggc aggcgcggct ggggctgatg tacacttctc
    13441 aatttggatt ggctgatcac aactatatcg gtttagcaat gccggctggt gtccattatt
    13501 cactggcttt gagctggatt cgctatggag tggacgatat tccgattcgg cctgatattt
    13561 ttactacaat taccgataag gctgaacggc gtgattcgat tattagtatt tataacggca
    13621 aattgccaac tttcaatgat gtggaaaatg ccgtgtttgt ctcgcttggg cgatttaatt
    13681 cgactatagt tgatttaggt tggcgctatt cgaaattcaa tctggagttt ccaatcggga
    13741 taaatttaaa attcatccat aaaaaatatt acgacctcga aggtttcgga ctgggagttg
    13801 atattggcgg acggttgcgg gttgatggcg aggagttgct ggatgtaagt aatttgggga
    13861 tgttgagtgt tggaatggct ttgaaagacg tcactggtac aaccatttac tggaatacta
    13921 agagtcagga tcgtattcgg ttgaccccgg cgttgtcatt tgccttggaa cagccgatta
    13981 aaccattaaa attgattata aatctagttt ctgaaaaaca atatcgttac catgataaaa
    14041 ccaattacgg cgttgaaatt atttacgcta atagtctggc gctacgagct ggcttaaaaa
    14101 attctggcat aagtctcgga ttaggtttaa attttaaagt tatgaagaaa ctaattaact
    14161 tagattattc attttatagt catgatctcg gagcgagcca ccgtgtcggg cttggaattg
    14221 ttatttagta aaaatgacga cgtcgaacca aattaattta atggtggctt taattttact
    14281 tcttaatacc tgtggaaaaa atctggaaga tccggatccg ccagctcaac cggaatgggt
    14341 agcaaaatcc ttaccgtatg atacgaccga gaccggcatt gatgccgttc ctaatggtga
    14401 ttttatccaa ttagaatggt ataccggcga tgaggacgac cttagcagtt accggattta
    14461 tcgggctagc ggagatattg aaaataaatt tgatctgata gaggaaatcc aatctgactt
    14521 aatggcaggg agtgtcaata tctatctcga cgaaaatgtt tctattaata ccccatattt
    14581 ttattttctg cgcgccgtag accaggctgg aaatcttagt ctgcgttcgg atacggtaga
    14641 gtataagcta gtggttaaag taaatttacg tggactattt gatactactg atgtgaaccc
    14701 aactttcaaa tggtcggatg ctgggtcggc cgcttctgaa tatgtcatcc gagtagaaca
    14761 gtttaatccg aatcgggtga tgtgtttgtc agctacatct cgacaaagtt ataccgatga
    14821 agttcagagt ttaaaatatg gaaccgggat tattttctat tcagctcaag atacgctaag
    14881 tcgaggagtt gattaccgct ggcgcgttga cgcagtggcg actttcaatc gtgaaggcga
    14941 tgaggtcgcc ggttcggaat ccaattgggg atattttaaa attcaataat aattaggagt
    15001 aattgtgaaa agagtttctt tatttatgat tttaacctta ttaagcgtcc aactttattc
    15061 tcaggaaagt cagacgatta ctactagatc cggtcgaaca gccgatcggt tgtccgcatc
    15121 caagtatatt ctgtcaactg gttcagacgc tatgttaatg actgtcaaaa tttggggcga
    15181 agtaactaga ccaggccttt acgatgtacc gatcggcacc gatttgattg atttaatttc
    15241 agccgctggc ggtccaacta ctcgtgcaaa tttatccaaa gtcaaattaa tccacggttc
    15301 accggacgag gaagagaatt acgtaactat tgttaatatc aaaaaatttc tggaaaccgg
    15361 tgatgtggat atgattcctg aaatcaaacc gtccgatacg attgtggttc cggtcaagat
    15421 tacgcaacat tttctgacgt cattatcgtg gacgcaacaa ttattaagtt tgttctcgat
    15481 ttatagtatg atactttatt acacaagtat agcaaatagg taaaaccata ggagtgatac
    15541 atgattgaca gtattgacgt ccaaatttta gatattttgc agaaggaagg ccgtattcag
    15601 cgcaataaaa ttgcggagca ggtcaacctg accattcccg ctgttagcga aaggatgcgg
    15661 aaattagaaa aaaaaggcat tatcgaaggc tatcacgcta aaattaatag cgcggccatc
    15721 ggaaaggatg tgacggcttt tgtttttgta atgacttcgc catcgaagaa ttatgatgaa
    15781 tttgtacgtc ggagtcgtga agaaagtgat ataatagaat gtcatagtat caccggtgaa
    15841 ggctcgcatc tcctgaaagt acagactgat aatacctcag ccctcgaaaa attgctctca
    15901 aaaattcagt cctggccggg tgtattacaa actcgcacct acatcgtact ttcgagttat
    15961 aaggactttt catcgctgaa gccggcaagc tcgttagtta agaagtaaat tttcctcgtg
    16021 caatttgccg tttttattat taaggttaaa aatacttaat taaggtcgaa tattaaaatg
    16081 ttgagtgata tccaagttga attagataat attgcgcccc gcattgatga attgcggagg
    16141 tttctttgac gtcgagtcga agaaaaatga attgaatacc gagcgtcaga aaacgaccgc
    16201 ctcggatttt tggtctgatc cagcccaagc gcaaacaact cttcaaaaaa ttcgggggct
    16261 ggaaagcgaa atcgataatt ttgaaaatct ggtgaaatat caaaaagaaa tcacagactt
    16321 cgtggattta gccgctgagg aacaagatga ctctgtcttg gctgaattgg aaaagatggt
    16381 aaacgaactt attagtaaag tcgaacaatt agaattggaa acaatgctcg atgaaccgga
    16441 cgatgagaag aatgctattt tgacaatcca tccgggcgcc ggcggaaccg agtcacagga
    16501 ttgggcgcat atgctgatgc gtatgtattt gcgctggatg gaacgcagtg ggatggaggc
    16561 ggacgtcatc agtattctcg atggtgaaga agccgggatt aaggaagtta gtatcgaagt
    16621 caaaggtaga tacgcctatg gctatctcaa agccgaagcc ggaattcatc gcctggtgcg
    16681 catttcacca ttcgacgcca accatcgtcg ccatacttcc ttcgcttcgg tttttgtcta
    16741 tccggcaatc gaagacgata tatcggtcga aatcaatcag gatgacctgc ggattgacac
    16801 cttccgcgcc agcggtgccg gcggtcaaca tgtcaataaa accagttctg cggttcggat
    16861 tactcatata ccaaccggta tcgttgtcca gtgtcaaaac gagcgttccc agttcaaaaa
    16921 caaagatagt gcgatgaaaa ttctaagagc gcgcctctat caattaaaaa aggaagaaaa
    16981 aaataaagaa aagatggaga tcgagaagaa taaaaaggat atttcctggg gcaatcagat
    17041 tcgctcctac gtttttcagc catacacttt agtcaaggat catcgcacga agtacgaaac
    17101 cggcaatatt caagccgtga tggacggcga gatagatgaa ttcctgcgtc agtatttatt
    17161 attcaatgtc aaaaatgaaa gagctaaaaa gtgaataaag aagaacaagc aactggaagg
    17221 tcattaaacg aactactcgc gattcgtcgc gaaaaactgc aggcaatccg tgaaatgaat
    17281 ttccgggcgt acgaatatga atttcaaaaa acgcattcgg cggctgaaat aacccagaat
    17341 tttgatgaat taactgaaaa agtagtcgta aaagttgccg gacggcttat ggcaatccgt
    17401 aaaatgggac gcgccagttt cggccatatc caggatgaaa gtggacgaat tcaaatttat
    17461 gttcaagaaa acagtgtcgg tcagcaaggc tataaattat tcaatttact tgacatcggc
    17521 gatattatcg ggattaccgg cagagtcttt cggactcaca cgggcgagat tacaattcta
    17581 gttgaagagt tgcaattgtt ggctaaaaac ctccgcccaa ttccgattgt caaggaaaaa
    17641 gacggacaaa ttttcgatgc ttttagcgac aaagaacaac gctaccgtca gcgttatctc
    17701 gatttgatgg taaatccgga agtgcgggag gtttttaaaa ctcgttccaa aattattcaa
    17761 tggacgcgag attttctcaa tgaacacggt tttctagaag ttgatacacc aatccttcag
    17821 ccgatatatg gcggtgctaa tgcccgcccc ttcaagactt attataatgc tctcgagcgc
    17881 gaattttact tgcgaatagc ggttgaactc tatcttaagc gcttaatcat tggcggtttt
    17941 gaaaaagtct atgaaatcgg taaaaatttc cggaacgagg ggcttgaccg cagtcataat
    18001 cccgaattta ccttgcttga attttatcaa acttatgttg attataatta tatgatggat
    18061 tttgtcgaac agtatttcca ttatctttgt tcgaagctgg gggaatctaa atttcctttc
    18121 ggtgaatacg aaattgactt cagtcaaccg tttagccgcc gaaaaatgtt cgatttaatt
    18181 cacgaatatg ttggcgaaga tattacccaa tattcagaag ctgaattgaa gagtttatgc
    18241 ctgaagaaaa acctcgagtt gaaaccaggc gacaattacg gtaaatatat tgaattactg
    18301 ttctctaatt tcgttgaacc gcatttgatc cagccgactt atgtaattga ctatccgaaa
    18361 gccatttcac cactggctaa aaacaaacgc gacggttcac caaatctcgt cgaacgtttt
    18421 gaactttata tcgccggtca cgaatttgtc aatgctttca gtgaactgaa tgatcccatt
    18481 gatcagcgtg aacgtctgga acagcagaat cggctgaggg aatctggcga cgaggaagcc
    18541 cagacgatgg atgaagattt tgttacggcg ctggaatatg gaatgccgcc gaccggtgga
    18601 gtagggatcg gtattgaccg agtcgtaatg ctatttacta atcaggtttc gattaaggac
    18661 gttattcttt ttcctcagat gcgtacctga gaaagtaaag cgatgagcgt accttttttc
    18721 atcgccaagc accatctttt cagtcgtcac aaaatcggct atatatcctt cataagtatt
    18781 atcgctacca ttagcttggc ggtcggagtg gcggctttaa ttttgacgat ctcgatcctg
    18841 aacggtttcg aacaggaaat caagaccaaa ttactcgggt tcgattccca tatccgtttg
    18901 cgtttacttt atcaggattc aatggactcg acgctggcga ttcggtcaga gctggaaaaa
    18961 atgccggaga tcgaatgttt cgtgccctat attcacggag ccgcgatgat ccgcaaaggg
    19021 caggaaaccg aaggaataat cgtcgaggga attgacgaag ttcacattcg acaaactctg
    19081 gcggttgacc gctttattaa aaaaggcgaa ctgcgtttta atttgccgga tggtcgtgag
    19141 ggtatcttaa tcggcgcgaa attagccagg caactcggcg tagatattgg tgataaggtt
    19201 tacctcttcg tatttcaacc ggaagagggt tatatcaaac ggccgcgcat tgtgactttt
    19261 acggtgaccg gtctgtatga ctcgggcatt gctgattacg atgacgtttt tatctatacc
    19321 agtttgcaag cggcgcagaa gctctatgat ttgggtgaaa aattttctgg ttatcaaatc
    19381 cggctaaaag atcagtccaa agtcgaagcc gtgaccgaat atattaacaa aactctcggc
    19441 tatccctata atgcgctcag ttggaatgat ctacactcca atcttttcga atggctgagg
    19501 attcagcgca tcccgatact gctgattttc agtttgattg cggtcgtagc cattttcaat
    19561 attgttagct caataatgat gattgtgatc gaaaaaactc gcgacatcgg catccttaag
    19621 agtgtgggaa tgaataatag tcaggtgaag cgcatatttt tatatgaagg gctttttatc
    19681 ggagttgtag gaactttggc tggatttggg ctgagtatat tattaggtta tctccagaac
    19741 cggtttaatc taatttccat cccggcggat gtttatttta tgagttccct gccggtattg
    19801 ttagagttgc ggaatttttt aataatcggg ttaattgcga tagcttcggc gctattagcc
    19861 acgctttatc cgtcgtataa agccgttaaa ttgactccca gtgaggcgac ccgttatgaa
    19921 taagaatttt ctgaactgga tcgctttacg ttatttccgg tctaaaaaga agaccggttt
    19981 gatttctttt acgtcttata tctccattat cggcatcgca ctgggagcgt ttgcgatgct
    20041 ggtggctttg agtgtgttaa acggttttga ggcggaaatc accaatagag tcatcgacat
    20101 cgaatcacat ctgcggatta gcggacgcaa tctccaatca gctcaaattg aacaggttaa
    20161 agaagcgcta tccggtgtgc cagtgcgcga gatttatcca tttgtaatga aaaagagcat
    20221 attgtcttcg gaacaaatgg aagccgtggt gcggattaaa gccatcgagt ctgctacact
    20281 ggaacaacaa ctttccggcc cgcggactat gatgcgcggt ggggcaaatt tcttatcggc
    20341 aacttcgaat ctacccggca ttgtggttgg ttatcaatta gccgataaac tcggtgtcta
    20401 tccgggcgat actttaaatg tgattaatcc aaccagcctg ggaagcggct tgaatatccc
    20461 aattatcggt caatttgccg tcactggagt ttttcaactg gacttattcg attatgatga
    20521 gaatatagct tttatcgaat taaaagtcgg tcaggaaata ttcgaaatgg gagataatta
    20581 cagcggaatt gacattcgtt tggaaaatta tcgctacgtc gagaaagcca agcaacaatt
    20641 ggttgctgaa cttggtaacg aatatgatgt ggcttcttgg gaagatttgc atcgtacctt
    20701 gtttggagca atgaaattag aaaaatacgg cagttttctg gcacttagtt tcattattct
    20761 ggtggcgatc ctgaacttga ccagtgcatt ggtgatgctg atcatggaaa aaatccagga
    20821 aatcggtatg ctccaagcac tgggtatgaa ccagactcaa gttcgccaga tttttcttag
    20881 gctgggattt ttaatcggga gcatcggttt gacggttgga atagggctgg cattaatcct
    20941 ttgtctggcg caacagcatt ttcaatttat accgcttccg tcggtatatt atattcctta
    21001 tctgccggta caggtgcaat ggagtgacgt tttaatgatc atcagcgccg gtttgatctt
    21061 catctattgc ggaacgcttt atcccagctg gcgagttggt aaacttatgc ctttggaggc
    21121 tattcaatat gaaaaatgag aatgacaaaa ccttattaac cgtgaagaat ctatcgaaat
    21181 catactggaa cgacggcttg gaaattgccg tgctccgcga tattgattta acggttaagg
    21241 aaggtgaagt tgtggcaatc gttgggccat ccggcattgg gaaatcaact ctgctgaatc
    21301 tattaggtac tctcgaccga ccggattccg gcgtcataga atatgagggg cttaatccgt
    21361 ttgatttatc agatacggaa attgcgcgct tccgcaatac tcacattgga tttgtttttc
    21421 aatttcatca ccttttgccg gaatttacgg ctctcgaaaa tgttatgatt ccggctatga
    21481 tttataaccg agatgtcaga aaagcggaag aaatggcacg tgaaatgctt gcgcgagtcg
    21541 ggctcgctga acgggctacc catcggccgg cggcattgtc aggtggtgaa cgccagcgag
    21601 tagccgtcgc tcgggcgcta atcaataatc ctttgattat tttagcagat gaaccgactg
    21661 gcaatctcga tgcgatgaac agtgagctat tgattgaatt aatgttagaa ttgaatcgga
    21721 tatatcggcg gacttttatc attgctactc ataatcagac aattgccgaa aaatcccacc
    21781 gcattttgaa attcaccaag gattctctgc ttatcgaagc cagcatttaa ctacccaaat
    21841 aagttaggtg gttaattact gaaaaatgct acgccaaagt ggcaaagatt ttgcataaca
    21901 taaaaaagga aagaaaagat tgacttagca gtcagagagt tttaacttct gatgttattt
    21961 gagaaagaat tgaaataatg aaagagaatt tttccaagat aattcaaatt attgtcaaag
    22021 ccgcccgcta tgaagcaggc cggatgaatc atagttatgt tggctccgaa cacttgctct
    22081 gggcgataat caatatggaa cgaagcgtta ctcttgagat attgatggcg ctcgatgttg
    22141 atatcaaagc cttacaaact atgctgactg attcattaaa agcgatcggc ggaacggcga
    22201 caatcggaca gattccgttt actaaacagg tggagcggat tcttcgtaat acttataacg
    22261 aagctaagga actgggcgcc gaagtcgttg gtgatgaaca tattactcta tcaattttga
    22321 aagaaaagga gggaatttcc tacgaaattc tcaatagttt tggcgttgat tattataatt
    22381 ttcgggctga aatttcagga aatatggatg agccacttcc cgttacaaaa agtgagaaaa
    22441 agagttcacg aacaccggca cttgaccact tctctcggga tataacggaa aaagcccgga
    22501 ctaaccaact tgatccagtg attggtcgcg atcaagaaat tgaacgagta gctcagattt
    22561 tgtcacgtcg caagaaaaac aatccagtac taatcggcga gccaggtgtt ggcaaaactg
    22621 caattgcgga gggattagct ctgcgaattg tggctaaaaa agttccgcat ctacttgcga
    22681 acaagcggat cattgatttg gatttggccg cgatcgtcgc cggtaccaaa tatcgtggtc
    22741 aattcgaaga acgtatgaaa accattatgc aggaactcga gcaggcgcgc gatgtgattc
    22801 tctttataga tgaactgcat accatagttg gagcaggggg agcgtccggt tcgctggatg
    22861 cctctaatat gttcaaaccg gctttagcgc gcggtgagtt acagtgtatt ggtgcgacta
    22921 cgctcgacga atatcgcaaa tttattgaaa aagatggcgc gttagaaaga cgtttccaaa
    22981 aagtcataat taatcctcca agtaaagaag aatcgctgga aatcctaaaa ggattacggg
    23041 agcgctatga agcccatcat cacgtcaaat ataccgatga agctttatgg acggcggtcg
    23101 aactcagtga ccgttatatt actgataaat ttttgcctga taaagccatt gatgttatgg
    23161 acgaagccgg ctcgcgggtt catctacgag atgttattat gccggaggaa atacttgagg
    23221 ctgaaaagaa actggaagaa gtccgcaatc agaaggataa tgccgttaga gaacagcagt
    23281 ttgaaaaagc cgctgacctg cgggacagcg aacggcgggt taaaaaacaa ttagccgatt
    23341 tacagcaagc ctgggaaaaa agtggcgaaa aacaaacctt attagttact gccgaggata
    23401 ttgctgatgt agtagcgatg atgaccggga ttccggttaa cagaattgcc gaatcggaaa
    23461 atcaaaaatt actgacgatg gaagatgaac tccgcaagtc aattatcggc caggacgatg
    23521 caattcgagt cgtaagtagt gcgattcgcc gtgcccgtgc cggcttgaaa gatccgcggc
    23581 gcccaatcgg atctttctta tttttaggtc caacaggcgt tggaaagacc gaatttgcca
    23641 gagtactggc tcgcttctta tttcccggca cagatgctct gattaaaatt gatatgtcgg
    23701 agtatatgga aaaatttgca gtttcgcgac taattggggc gcctccggga tatgtcggtt
    23761 atgaagaggg tggtgaattg actgaacggg ttcggcgtca accctattca ctggtgcttt
    23821 tcgatgagat tgaaaaagcc catcgcgacg tttttaatat tttactgcag attttcgatg
    23881 aaggcgtttt gactgatagt tttggtcgtc gggttgattt taaaaatacg atcataatta
    23941 tgacttccaa cgctggttcg cgcagtctcc agagctcggc gtttggtttt tccaaagccg
    24001 atccgcttag cgaatatgag gctatgaaaa aacgcttgct ggaagaaatt caacatgtat
    24061 tcaatccgga attcttaaac cggattgatg atccgatcgt ctttaaaccc ttaactcgtg
    24121 aagacgtttt agcgattatt gatttgcaat tggaggatac tcgccgcaat ctaggagctt
    24181 cagggacgac cttagaaatc actcaggaag ccaaagaact aattcttacg cagggattta
    24241 agcccgaatt tggagcgcgt ccactacgtc gggcaattca aaatatgatc gaggatccga
    24301 tttcagaatt gattctggct ggcactttaa gacgcgggag taaaatcgtg gttagtgtta
    24361 ctgataacaa gatcgtcttt actcaacctg aaatagaatt taaaatttct tccacgaaaa
    24421 ttacgagcga accggtcagc aattaagtca taatttcagt cttttcaaac caaatatagt
    24481 atttagtaag atagtctgac gttgtgtcct aataactatt tggcacgagg tttgtcaatc
    24541 ttttagtgaa atttgaaaaa ttgaagccca aaaaaaggag ataaatatgg gaaaaattat
    24601 aggtattgat ttaggaacaa ccaattcttg tgtggctgtg atggaaggtg gtaagccacg
    24661 tgttcttgaa aattccgaag gacgtccggt gacgccatcc gtggtagttt ttaaaaaaga
    24721 tggcgaaata gtagttggtg atactgccaa acgtcaaatc gtaaccaacc acgatcgcgc
    24781 cgtttattcg attaaacgtt ttatgggacg tttgtataaa gaagttcaac gagaacgtga
    24841 agaagttccg tttaaagtag ttgaaggccc taatggagat gctcgaatac gaattggcga
    24901 tcgggattat tcaccaccag aaatcagtgc aatgattctg cagaaaatga agcaaaccgc
    24961 tgaagaaaaa ttaggcgaaa aaattaccga tgcggttatt accgtaccgg cttacttcaa
    25021 tgattctcag cggcaagcta ccaaagatgc cggtaaaatt gccggtttgg aagtaaaacg
    25081 tatcatcaat gaaccgacgg cagccgcttt ggcttatggt cttgataaga aaaaggaaga
    25141 aaaaattgcc gtttatgatt tgggtggcgg tacatttgat atttccatcc tagaaattgg
    25201 agatggtgtt tttgaagtaa aatccactaa tggcgacacc cacttgggtg gcgatgattt
    25261 cgatcagcgt attattgagt ggttgattag tgaatttaaa aagaaagaag ggattgatct
    25321 taaaaaagat ccgatggcaa tgcagagact taaagaagcc gctgagaaag ctaaaattga
    25381 actttcttca aatctggaaa cggaaatcaa tttaccctat attacagctg gttctgctgg
    25441 tcctatccat ctaatggaaa aattgtcacg agcgaaattt gaatctctcg ttgatgattt
    25501 gattgaacgc actcgtaagc cgtgtcagca agcattggaa gatgccggat taaaaccgga
    25561 agatataaat gaggtaatcc tagtaggcgg ttccactcgc atacccaaag tgcaggatct
    25621 agtcaaagac ctttttaaga aggaacctaa taagagcgtc aatccggatg aagtagttgc
    25681 aatcggagcg gcaattcagg gtggtgtttt agcaggagaa gttcacgata ttctactgct
    25741 ggacgtaacg cccttgtcac tgggaattga aacactgggt ggagttacga ctaaattgat
    25801 tgagcgtaat acgaccattc cgacccgaaa gagtgaaatt ttctcgactg ccgcagataa
    25861 tcaaaccagc gtagaaatca acgttctcca gggtgagcgg gcaatggcgg ccgataacaa
    25921 gagcattggg cgcttccatc tcgatggtat tccaccagca ccacgtggtg ttcctcaaat
    25981 tgaggtcact tttgacattg atgccaatgg tattctgaac gttaccgcta aagataaagc
    26041 taccggcaaa gaacaaagcg tccgaattca aacttccagt ggtttaaccg aggaagagaa
    26101 agaaaaaatg gtacgcgatg ctcaactgca tgaagaagaa gatcgccgtc gcaaagaaga
    26161 agtcgatact cgcaatcagg ttgatgcctt gatttaccag acagaaaaga atatgaaaga
    26221 atacggcgat aaactgcaac cggaagaaaa aggcagactg aacaacgcac tggaagactt
    26281 gaaaaaagcc cagaatagtg gtgatgtaaa aacgatgaag agtgcgatcg aagccatgaa
    26341 tcaagcctgg tcagaagtat catctaagat gtacgaaaga gtacgcacgg aaacaccacc
    26401 accacatact ggtcagactt atgaacaacc acatgaggaa acgaccggtc aaaaagatga
    26461 aaacattcaa gatgctgatt ttgaagtagt ggacgacgat aagaagtaaa tgaattcgta
    26521 aatggtaaaa agggcggctt tccgcccttt tttattatca gcccttgaaa ctggaaaagg
    26581 ttttgttttt ttgagtaata aaatctaatt ttcaccgctt tttttaattg gcgacgtacc
    26641 caagtggcta agggagcggt ctgcaaaacc gttatgcacc ggttcaagtc cggtcgtcgc
    26701 ctctgatttg agcagttttt tcatatagtt cattttgccg aagtggcgga aatggtagac
    26761 gcaagggact taaaatccct cgatcctttg gatcgtgccg gttcgattcc ggccttcggc
    26821 acaactttca taataagaga ctaagagtct ccttttattt taaaagttag aatagaatga
    26881 tatctcaaac actaccattt gtatctacga ttaatcatcc tgaatttgaa tttgaattta
    26941 attattgatt ttacacataa agtaaagtaa aatacatatt atttgaaatt taagagaatg
    27001 aggatcaagc gatcacctat aacacgatct cgcaggcctt tttttatgtt tactgctgga
    27061 tcataaccac agctcagaag gagttaaaaa atggaatcat ctaaaaaaca atcggtaatc
    27121 aacgaaaaac ccactcctat caaaggacta acgacagagg aagcgctttc tcggctaaaa
    27181 aaatatggtc ctaatacgtt cccaagaagt aaaccaccct cagcttggtc aatcctaatt
    27241 tctcaattta aaagccccct tatttatata atcctggtag ccgctgtgat ttcactcatt
    27301 atgggcgaac tgactgattt cattataatt atagccgtcg ttattgttga tgtcattctc
    27361 ggatttttcc aggaatttca ggctcaacgt acttatacgg cattgaataa attgctaaaa
    27421 ccgaccacta ctgttatccg cgatggagaa cgccaggaga ttgatgtaac gaaagtagta
    27481 ccgggtgata tcgttatact aaatgcagga gagcgtattc ccggtgacgg tgaaattatt
    27541 gagggcgcca ggctcagtgt agacgaggca atcctgaccg gcgagagtga gccgattaaa
    27601 aagtcagtcg aagccggctc taaccaggtt ttcatgggta caacactcct tactggtaga
    27661 ggagtaatgc gagtcaccca aaccggtgtt cgaaccgagt taggcaaaat tgccaaaagc
    27721 cttcaggaac ataaagaaga agagactccg ttacaggtac gacttaaatc tttcagcagg
    27781 acgttgacaa ttattgtcgt ttcgttcact actattatat ttctcgtcgg acttttactg
    27841 aagcgtgatt ttctggatat gctacggact tcggttattt tggctatttc ggcaataccg
    27901 gagggcttgt tgattgctgt tactgttatt ttggtactgg ggatgcgtaa gatccttaag
    27961 cgccaaggct tagttaagaa actgttagcc gttgaaactt taggttctgt caatgtcatc
    28021 tgtaccgata agacgggtac tcttaccgaa ggccgaatgc gtgtgactcg taccgatttt
    28081 gtggataacg agcgagccat ccagattatg gttctgtgta ataaccttga gggaccggtt
    28141 gaaatggctt tgtgggagta tgccaaatct cgactgccga atgatcccca gactttagtt
    28201 gaagagagcc aacgtctgga tgaagaaata ttcaccagtg aaaccaaatt tatgattact
    28261 ggtcaacact tacctgatgg cgattatcac ttcctgaagg gtgcgcccga aattgtcctg
    28321 aatatgtgtc aaatcaacga tggtgaacgt cagcgaattt tggctcaaat tgatcaatgg
    28381 gctggtgaag gattgcgtct tctcggtctg gcttaccgtg actatggaat cctggaagat
    28441 cataccggtt atagatgggt aggcttgatc ggtatggaag acccgattcg cgagggagtg
    28501 cacgaggcgg ttctactcgc taaacatgca gggattcagg ttaaaatgat aaccggcgat
    28561 tatcggcgca cggccgaacg tatcgccagt aatattggtc tattgcaaaa cggtgaggaa
    28621 acactggaag gcgaagaact tgctcaatta aatgacgagg aacttgctaa acgtatatac
    28681 cctaccgccg tttttgcccg gattaggcct catgataaat tacggatagt taacgctttg
    28741 cagaaacacg gcgatattac ggctatgatc ggggatggcg tcaatgacgc accggcactt
    28801 aaacaggcta atatcggcgt ggttgtcggc acagccagcg atgtggctaa agagaccgcc
    28861 gatttgattc ttctcgataa caattttcgc accatcgttg ccgcaatcga ggaagggcgc
    28921 gtgatctttg agaacatccg caaagtcgta gcctatgtcc tctccaattc tttcgccgag
    28981 gtattgacta tttttatatc tatgattttg ggctggccgg cgccgctatt ggttgcgcag
    29041 atactctgga ttcacctgat ctgtgatggt ccggctgata tcgtgctcgg ttttgagccg
    29101 aaggagaatg gcattatgga cgaaaagcca aaatctctta aagaaccaat cttgcctcct
    29161 ttaggcttgt ttttaattgg tgtgattagt atcggcagtg cgctagcggc tctcctgatt
    29221 tttggccact attttaaaat acacggtgat gctgacgaag gccgtagttt agtcttcgct
    29281 agcctggcga taaattcact gatttacatt ttcgcttacc gaagtatgcg caagtcactt
    29341 tttaaaatga actcgatcct gaaaaacaag ccgttaatct ttgccgtaat ttttggaatg
    29401 gtgctggttt ttctgccttt tgtaataccc agtttaagcc aagctttgga cgttgtaccc
    29461 ctggacctga aacaatggtt attgatcttc acgatagcat taatgcttct tattgcggtg
    29521 gaaattggca aggcagtcat caactggtgg cacaataaaa aaatactctg actaaaggat
    29581 aaaaatgttt atcctaatag aaaatgcaac taaaactcgt ttttgggtct aatctcagca
    29641 atattgttta tatttgctta cttaatttga tattttaaat tactttgttt attttggaat
    29701 tagttaattg attgttgaaa gcaactatgg ccaaaattga tccaaactcc gattactatt
    29761 ctgaactctc ccagtttacg atcgagagtg atgtggatgc tattctttgg atcgatgctg
    29821 agagtacgat ttaccgggtc aatcaagccg cttgtcaatt actgcaatat tcgccagaag
    29881 aattggtcgg taaaaattta gatgatctaa ttgacattca gaatgattgg aataaaaccg
    29941 acccacaccg ctttttagac cagattgata tcataaaaca aaatggtgtc acagttatcg
    30001 agcgcaattt ccgtacgaaa gacggtcgga ttctgacttt ggaaatctcg gcgcatttat
    30061 ttgtttttaa aaataatgaa tatatctgcg tatttgcccg ggatataact gaacggagga
    30121 aagcccagga agagctgcaa aagacactga aggaattgga atttttaaaa aataaattat
    30181 atgaagaaaa tatttacctt cagaatgaaa ttaaactaac tcacaacttt gaagaaattg
    30241 tcggcaaaag tctgagtctc agaaaagttc tggaaaaagt ggaacaggta gccgcaaccg
    30301 acgctaccgt tttaatacaa ggcgagacag gaaccgggaa agaacttatt gcccgcacta
    30361 ttcatcatat tagttctcgt agttccagtc cattagtcaa ggtaaattgt gctactttac
    30421 cagccgcctt aatcgaaagc gaattattcg gacatgagaa aggagctttt accggggcac
    30481 tttatcgtaa aatgggacgt tttgaactgg ctaacggcgg tacaattttt ctggatgaaa
    30541 ttggtgattt accactggaa attcaatcta aacttttacg agtaattcag gaaggtgaat
    30601 ttgaacggat tggaagtaac aagaccctca aaaccgacgt acgtataata gcagccacta
    30661 atcgtaatct tgaaaccgct gtccaaaaag gtgaatttcg taaagacctt tattaccggt
    30721 tgaatgtttt tcccattcat gtgccacctc tgcgcgaaag gccagaagat attcctctct
    30781 taatacatca ttttgtccaa aaatattcga agaaaatcgg tcgcaaaata gatattatac
    30841 cagctaaaat aatgcgtact ttaacaagct atagttggcc aggaaatatc cgggaactag
    30901 aaaatattat cgaaagagcc gttatcatta ccaacgggcg aactcttcag attggtaatt
    30961 ggtttacaac tgatgcgcca gtgatgaata aggagaaaat tcaacccttg cgtgaagccg
    31021 aaagggatta catcttgaaa gtactatctt tgacaaactg gcgtgtcagc ggacctaacg
    31081 gtgcggctca actcctggat atcaatcccc aaacgttagt ctccaagatg aagaaattgg
    31141 gcattcatcg cactaaacca attaatgaat aataataaat tatatgatat atcataaaat
    31201 aatcaattgc taaacccagc aatttttgat cgaattatta tatcattctt caattaatag
    31261 actattatca aacaaagccg ctgatttggt atagattttg tctttattaa aattgtatga
    31321 agatgaaaaa agaaaataag agtgtaaaaa aagaagatac taaaatcatt gatatgtcga
    31381 cttttgatga gttgattcat agcaacaaac cggtggtggt cgaattttac gatgaaaaaa
    31441 ttagcacgag ttatatagtt gattcgatat tgtcccggct cgccaactat tttcagaacc
    31501 agattgaatt tttgaggctt catataaatc aacatcgagc aattgccgat aaatatcata
    31561 ttttttcatc accagccact ttaatctttt taaacggtaa actaatcgag gttatcctcg
    31621 ggactttttc gcagaaccag ataatcgaga ttctcaaccg tcttctttag taaaaaaagg
    31681 aggttaataa tatgacgact tttaagaaat acgtcaataa aattgcactc gacaaattag
    31741 taacctactg tatcaaagac ccgcagaagc atattcctaa actcattgat tttgttgata
    31801 aaagtgcctg gcttccaatt cataaaagct atgtacaagg agcaaaaagc attgtcaata
    31861 atcccgattc caactggtat caactagcta tgcgagcaat tagggatata gatccggata
    31921 ttatccataa aatagtgaga aatctgttta ttaattctgg cttcgaggga cagggtaagt
    31981 tagttgaaga gagggagagg ctcggttttg gtattccctg ggcggtgctt atggatccaa
    32041 ccagtgcttg taacttgaga tgtaaaggtt gctgggctag tgagtacgac aagagtctca
    32101 atcttgatac tgatctaatg gatcgaataa ttcgggaagg taaagaactt ggtatttact
    32161 tctatatcta cagtggtggt gaaccgctga tacgtaaaaa cgatttgatt aatctagctg
    32221 aaaagcactc ggattgcgtc tttctatctt ttacgaatgg cacattagtg gacgaacact
    32281 ttgcggatga attacgaaga gtaggaaatt tttcgctggc attaagcatt gaaggtgatg
    32341 aaaaagcaac cgatttccga agaggcaagg gcagttacca gaagctgatt tctgcaatga
    32401 aaattttgaa atcgagagga attccttttg gcttttcgac ctgctatcat agccagaact
    32461 ggaatatcgt gggggaaccg gaatatcttg atcagatgat tgaagccgga tgttattttg
    32521 gctggtactt tacttatatt ccacttggga aacacgctga tcttagtttg cttgcccgtc
    32581 cggaacatcg ggaacatatg tactaccgtg tgcgtgaggc gcgccagact aaaccaattt
    32641 tcttgataga tttctggaat gacggggaat atgtcaacgg ttgtattgcc ggtgggcgtc
    32701 gttatttgca tattaacgcc aatggcgatg tcgaaccttg tgcctttatt cattattcgg
    32761 ataccaacat taagaatgta tctttattag aggctttaca atctccactt tttctccaat
    32821 atcagaagaa tcaaccgttt aatcaaaatc acctgagacc atgtccattg ctggataatc
    32881 ctgacgcttt aaaatcaatt gtattggaaa ctggggcata ttcgactcaa catatggatc
    32941 gtgaggatgt ggtagaatta actgctaaat gtcaggatat agcgcaagcc tggaaactta
    33001 cagccgatag attatgggaa gagactctaa aaaatcgagt ccaaaagaag tccggagtga
    33061 aacaatatga gcgggaactc gttgatgcag aaatccataa ttgatataaa tgaaaagaga
    33121 cattctgtgg caagcgattt aggcatactg ggaatatggc ttttcatcag tgaactaatc
    33181 ggtggtactt tagtaaatag attaatttta ataattggcg gaattgattg ttatttcaca
    33241 actataactc ataaatagaa agtaagaatt ttttcctccc gagactcaat agtctctctt
    33301 cctcccaaaa agcggtcggt tatctctccg gccgcttttt ttataacgac taattaagaa
    33361 aaaccagaat gagatgaagg atttaatatt ttcttccgtc ttcattattc tttctaaaag
    33421 gtattaaacc tgctgttatg atattgatta ttcaacaatt tagaaattac ggcgaatgtc
    33481 ttcttagaaa aaaggttgac attattctct atcctcttta tccgatgagt agaagagaat
    33541 ttagtttgag tacgccagta agaatttgaa ctgaaaatac aatcagaaat tgagacgtaa
    33601 ccacggctaa aatttttatt ggtaaattaa aaagtgttaa atatatttga aacggttcaa
    33661 tgaataattc aaatttcata ttcaacgtta acattatgct cgaaaaatta gaattattaa
    33721 aagattgcat agttaaaaat tcctctctgt taatgataac attatgagag tatttccggt
    33781 tatttctgaa cgtaatcatt cagcaatatt tccgaatatc cgattctaag gaataggact
    33841 tatgacagct aatagccaag cggaaaacct cagccattta actttaaaag aagcttttaa
    33901 agatgatttc aaaatcgggg cggctcttga tttgaatcaa atttccggtc gtgatgagca
    33961 agggcttgcc attgtcgaaa agcattttaa tagcattacg cctgaaaata tattaaagtg
    34021 ggaagaagtc catccggaac cggaccgata taatttcgat gcggcggatc aattcgttgc
    34081 ctatggtgag aagcataata tgcaaatcat tggtcactgt ttagtttggt ttttccagac
    34141 gccggattgg gtttttgagg atggggcggg caagccatta agccgtgcag cattattagc
    34201 gcgtatgaaa gaacacattt ttacggtaat ggggcgttat aaaggtcgca ttcacggctg
    34261 ggatgtggtc aatgaagcgc ttatgataga tgggactttg cgaaagtgta aatggctcga
    34321 tattattggt gaagactata ttctcaaggc atttgaatat gcccatgaag ctgatcctga
    34381 ggccgaactt tactacaatg aatacgatac cgaacataaa tccaaacgtg aggcactcgt
    34441 tcgactaatt caggatttgc aggcgaaagg tgcccgcgta gatggaattg gtactcaggg
    34501 tcattggttt ctggattatc cttctcgaga ggaaatcgag aaggcgatct tagagatctc
    34561 cacaactaat gtcaaacaaa tgataacgga gctggatgtc ggtgtgcttc ctttttatcc
    34621 ggttgattcc cagatggtgg atatcagttc gttcgacccg gaaatgcaga aaaagtataa
    34681 tccttatccg gacggattac ctgattctgt tcaaaaagaa ttagcaaggc gttatgcgga
    34741 actctttacc atttttctta agcatagtga taaaattgac cgagtgacat tttgggcggt
    34801 tcacgacaaa caatcctggc gtagttattt gccaattaga ggaagggtgg aatatccgat
    34861 gttgtttgac cgacaatatc aacctaaacc agctctggaa gcaattttaa aagttgctca
    34921 ggatcgagct aaattagtgg ggaattagtt attgaaagat aggagcgaaa tggagaatcg
    34981 gttattctgt aatcaggatg cgatccgtcg ctataacgtt taattatttt aggaagaatt
    35041 acaaatggaa aagaaaaaaa atcttgaaat tgtcgccgcc gggcatactt gccttgattt
    35101 gatcccagcc tttacaattg agggagaagt tgaaaagatg acagatgtcc ttgtccccgg
    35161 caaaatgatc aatatgggtg aatgtgtgat tgccggtggc gggccggtta ctaatgccgg
    35221 ggtatcaatc cggcgtctgg gagtgaaaac cgaactaatt ggaaaagttg gaaatgatga
    35281 tttcggccgt cttgttttag actggtacga gaaaaatgaa ggtcatttta aaggtattaa
    35341 aatggttgat ggcgaatcaa cttcctatac catcgcaatc tgcattcccg gcattgaccg
    35401 tttttatttg catcattgcg gggctaatga tacctttggt tatgacgata tggattttaa
    35461 tttggtggga cgtgccaaat taatgctttt cggctatcca ccgtggatgc gtaaaatata
    35521 cgaaaacacc ggcgctgagc taactaaaat ccttaaaaaa accaaagagt tgggaactac
    35581 aacggctctc gacctttctc tgccagatgt tgacagttat gccggacagg ttgactggaa
    35641 agctatttta actgactggg taccgttatc ggatattatg gtgccgagtg cggaggaatt
    35701 gttctatttt ctatataaag ataaattctt ggaaaaaagg gacaaactta gacataagga
    35761 aaccgttctc gaccaaatga ccgttaagga aatttctacg atgggcaggg acctgataag
    35821 aatgggagcg gcgatcgcaa tgataaagtg cggtagtcgc ggcctttata tttacactgc
    35881 cggccaagaa cgcttgaaga agatgggcgc ggcgggatgt gccgaccttg agaattgggc
    35941 taatcgcgaa ctctggtttc cggtttatca ggaagaaaag ttcgttggcg cgctgggctc
    36001 cggtgattcg gctatcgccg gatttctctc agcctttgtc tgggggcata gcatcgaatc
    36061 ttgtttgcgc tatgctaatg ccgccggtag tatgaatgtt acggtgcctg acggtttaag
    36121 ctggaataaa ggatttgatg atttgaccag acgcatcaaa gccggttgga aaaccaaaga
    36181 gatgaaaata aacgaaccgg gctggaaaaa agaacgcgag ttctgggttg gtccggataa
    36241 tcgtggtaaa tgggaatcat aaaataatag gagaataaaa tgtcgaaaga gagagatgta
    36301 tttattcaga aaaaaatagc cggaatgact ttaatggaaa aaatcgggca gttgctgact
    36361 ttcacgcgca gaggaacgct tctgaccccg agtggaatcg agcagattac tcgtttgcat
    36421 tgtggtggtt tgtgtctcga accgtatgca gtcgagactt gcaaaaacct gtattggggc
    36481 aattcccaga tagataagaa ttttcaacca ccgaaagatt attttaccat cgctaataat
    36541 tattttgatg gaaaaacctt cggcatcagc attacgccgg aagattatac caaagactta
    36601 aaccgcttgc agaaaattgc tatgagccgg cctgcgggta ttcctctcca tatgaccatt
    36661 gattttgaag gtgattttaa aaatgattat atgtccggcg gtattcgtca gtttcccggt
    36721 ccgatgggaa tggcagctat cggtgatgag aaattgactt acgaaatcgg aaaaaccatt
    36781 gccagaatgt taacagcaat tggcgtaacc cagatgtact cgccggtctg tgacgtcaat
    36841 acaaatccaa gaaatccaga gattggtgtg cgctctttta gtgatgatcc tgaagtttgc
    36901 gctagacatg ccgtcgcgtt aattaaagga ttacaagatt acggtattgc ggcaaccgcc
    36961 aaacattttc ctggccgagg tgattcaggt tcggatgctc atgatgtgct ggaagtgcta
    37021 aatctcgaca agaaaagaat gcacgaaatt gagttagtgc ctttcaaagc ggctattgat
    37081 gccggagtca aggcgataat gaccggccat tccgtctacc cggcctttga cgacaaatat
    37141 cctactacct tgtcagaaaa aatcctgact ggtctcctgc gggaagagct gggattcgaa
    37201 ggcgtgattg tatccgatgc tatcggtatg gcggcaattt taaagcaatg gcctttacca
    37261 caagcctgcg caatggcaat caaagctggc tgtgatacta ttctcttaaa agccgatgat
    37321 gagtctcgct cacaatgttt cttcgggatt aagatggcgg tcgagcgagg cgaactgagt
    37381 gaggagagaa tcaatcagtc ggtcgcgcgt ctcctgaaaa tgaaatatga tcagggattg
    37441 tttgaatccg ccggagaaaa agaccctgag ataactcggg aaattaccac cgctaaagaa
    37501 gtaattgatt tctcttggga tgtggctaaa aaagccttaa tcgtaatgcg cgatgagcaa
    37561 caccttttgc cattggaccc ccagcagaga attttagtca ttgagcaacg cattccgtat
    37621 gagtttcttg gcaaagaccc ttattgccat acgcatatgt tttgcgaagc aatggtcaac
    37681 cacagtcaga atttgatctt ggcggataca gccttttcgg cttatgaaga tgagattaag
    37741 gagtgcctcg aattagccaa acaggctgat ctagtcgtta tgactaatta ctatgcccgc
    37801 attgtaaaat ctggtaataa tcggctttta gtcaagaaat tgaaggaagc cggacataaa
    37861 gtcattgtcg ttactaataa tccttacatc gaaggtacta cagtggatgc cgatgccgtc
    37921 gtctgcaatt tcagcggaac gccagattcg attcgagttt cgacttacct gctgtacggc
    37981 aaagtcatac cctgtccgac tactaaattg cccataaagc ctgatgtcgg ggagaaaacc
    38041 ccagttaagg atctcaaggc acctaaaaaa catccgctga atctttctta ttgttagatg
    38101 aagagatctg caagggaatg tactctatga agtatggcaa attatggaat tttttatatg
    38161 gtattgaata agaaattact aatgccacag acaggtaatt attgtatctt ttaattataa
    38221 atcgggaaga atagcatgga aaaaattaag attagcgacg tcttcactat ttccggaaga
    38281 caaatatcta agatttacaa agataacaaa atgacagtac actttcaggg aatgtccaag
    38341 actctgcaat ttccgcagac cattgctctg gaaggtgctc cgacttgttc aataggtatt
    38401 tctccggagg gtttatataa ccgccttaat gatgctgggc gattaatttt gctaaatcaa
    38461 aatgccatcc aatttggttc agccccgttt attaaaatca cggctgaccc aaatgctgaa
    38521 gacgaacgcg acgaatattt catgctaact accagagatc ctaatatcgt agaaggcccc
    38581 gatgatgtac cggcattaaa aaattactat catccgccag ttcccgaagc aaaagtaacc
    38641 atttcactgg ctactccctt ggcagagatt atttatgatt tggcgaaaat aagtgtaact
    38701 cgccttatta cctcgccttt attttccgga aatgaccaga cccttattcc tattagcgta
    38761 gaagaatttg tagtcgagaa tacttccgat aaaactcaag aagtgaccct agtaataccg
    38821 cgtccctcac tggtaaatct tcaggaaaaa gaactaaaac caaccgatca agatacggta
    38881 tatatttgct cggcttcagt caaagggcag gtgcatgaag cctttaattc agccggaatc
    38941 aaaggtgtcg tcatgggcaa tcaagagtgc caggaaaaaa tggcgatcgc tgttcctgaa
    39001 atagagggtg ttgatattga tactcacccg catttctgcc ttaatcattt gaaacaggat
    39061 ctgttgctta acgacgatgg cagtttttat gaaaaacgta atgtaattcc gcatcaggat
    39121 tatggcgcgg cgattagttt acattttact ctgggaccta aagccagtct aaaaattccc
    39181 tttgcaattg ctctggattt tccccaacag cgttttatcg atggggtaga atttgagcgg
    39241 aagtatgtca aattattcaa gaatcaagcc agtcggacaa tagagatgat aaaagttgct
    39301 ctggaaaact accctgagtg gctgaatcga acattgacca tccaaaaccg gatttacgag
    39361 ctaattcgaa aaaatccagc ctatagagat gacgaagaag gtgctttgcg attagcccgc
    39421 ctgattttta atgaatttag ttgcccgata tcgaacgcct gtgtttgggt taaggataat
    39481 ggaggtaatg agagagcccg attcctcgaa tgctttgatt atgcttatat caatccttcg
    39541 gacgtagact ggtattcgat ggtgttgcta tttctcttcc ctaagattga ggaagaaatt
    39601 tgtcagggct tcattaactc aatcctagcg gaagatccga ccccgcgatt ttaccatttg
    39661 catgcttctt ttgttgaagc acgtcagcat tttgaaaaat atccagaaga gtacgaaggc
    39721 atttcgctaa ctcagatttg ggataccacc aaagtaaaag gcagtgtagc gcacgatctg
    39781 ggtgctttgc cgaaagggca cgcgctccgc aatgtcagtg attacacctg gtataataat
    39841 aattactgga tagatctttt ccctaaactg gcaacgcgcg tcctgcgcaa tgtcaaattc
    39901 accggcaata ctgcttttgt aaaggagaat tgggaaactc ttaaattcgg ttttgatcat
    39961 ttgcaagagc ttgatttcga tggagacggt atcccggaag ggtatcccgg cgaagtcaaa
    40021 aatacttttg ataatttgac gttatttggt gtagatgcct acgatgcgac catttttatg
    40081 gcaggttgtt atgcaatgat caaaatggct gagattgtaa aagataccgc ggcaaaaaag
    40141 cagtatgaag agatttttga taaagtttct aaagaatttg aaaaattatg gagagacacg
    40201 aaaaacagcc aaggcaaaag acttcaatac tatgtcacct gttttgatcc caagatggga
    40261 gaaaccaata ctgatgtctg gatgaatcaa ttggatgcca tctgggcatt gattgctatg
    40321 ggggaagagt cttttatacc cgaagagcgc attaagcaaa ttcttaaaac agtctatgat
    40381 aataatcgtg cttatatggg ctgggcaatg tgtcgaacag aagacggcgg aaaagttgaa
    40441 tcagaacagg gacaggatgt ctatactact tccaattatg tatttgcaca actgctagat
    40501 tattatggta tgacggagga aagtaaggaa gtatataagg cgatggacaa ggtgatcttt
    40561 ggacatggaa ataccctaat atcacctgat aatttgcgtg cggagctgga acaagaagca
    40621 ggcgaatcag cacctggtcc gcattatatc gttgccgctt atccgcgtcc tggggcaatt
    40681 ctgactcaaa tagtgctcca gtacattaaa gagacgcaga agcaaacgaa aaatatgctt
    40741 gtggattctg ataaattgaa gagttttgcg atttccttaa tgaaataaga gctttgatta
    40801 aaacggaaaa attacgaacg ttattaaaat tctaaaaaca gagggtgcat tatgaaagaa
    40861 aaaaccggag tgctttcact gaaagaaaaa atcggctaca gtttaggtga tacggcttca
    40921 aatctttttt tccagacttt tatcttcttt ttgctttact tttatactga tgtatttggt
    40981 attcccgcct catccgtcgc gacaatcttt ttaattggtc gaatttggga tgcggtcaat
    41041 gatccactaa tgggggcgat tgctgatcga actaatacca aaatgggtaa attccgaccg
    41101 tatttgcttt ataccgcctt gccctttggt atttttggct tcataatgtt cattaccccg
    41161 gatttaagcg ttcaggggaa agtgatttac gcttttatta cctataattt gatgatgatg
    41221 acctatacag tgatcaatgt accttattcg gctttaatgg gtgtaatatc accccattca
    41281 gaggaacgga ccgtggtctc gacgtttcgt tttgttgccg cctttgtagg ccagttcatc
    41341 gtccagtttg ccgtcctaag attagtgtct gtttttggaa aaggaaatga acaaannnnn
    41401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    41461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtcca gtttgccgtc ctaagattag
    41521 tgtctgtttt tggaaaagga aatgaacaaa tcggttggca atggactatg gcggtgatgg
    41581 ctggcttgtc ggttgttctc tggttagtga ccttcgccac gaccaaagag cgtgtcaaac
    41641 cggtcaaggg gcaaaagaac ccgttgaagc aggatttggc tgatctttcg aaaaatgtgc
    41701 catggcttct gattggctca gcgactattt ttcaactact gttcaacgtg attcgcggcg
    41761 gcagtattat gtattatttc aagtacttcg tccaggatca gcagttattt ttatttggta
    41821 aaccacatag ctactcctac cagactttga cctcaacttt tatgcttact ggtaccttaa
    41881 tcacaattgt tgggtcctta ttgaccagtt ttttcagtaa aaggattgga aaatcgagaa
    41941 gttattgggg ctttcttgga atttcggcta tctgctcagc tcttttcttt ttccttaagc
    42001 cgcaggatgt ggtctggatg tttttattgc aaataattgc ttcattttgt atgggtccgg
    42061 tttcggtgct acaatgggcg atttataccg atacggcaga ttattcggaa tggagaacgg
    42121 ggcgtcgcgc tacggcacta attatggctg tttcgctctt tgctctgaaa cttggcatag
    42181 ctctcggtgg ctccatttta gcctggattc tggattttta tgggtttgaa gctaatgtgg
    42241 ctcagacagc cagaagtctt accggaatcc gtatggtgat gagcgtttac cctgctattg
    42301 cggggatgat tggcgtcatt ctgatgacat tctatccact caataataaa atgatgttga
    42361 aaattgagga agatttaagt gccagaaggc agggaatagc cactgagtaa aatatacgca
    42421 ttcaaatcgg cgatcatagc gcagtaaaaa aaagtatcag ttttactagc gcggttctta
    42481 ggcatcctta gtttaatttt atagcggttt gagaaaagaa gagtattctt tattgaactc
    42541 attttataaa atcaacggca gaaattttca aaatcggctt ggttattcaa gcaaatggat
    42601 tgtacccagg aaggttatat tcctatggat agaaagttca ttgcatttgt cgattgaaaa
    42661 aatcactcat tttattttta tagaatgcat tggatttttc ggttaatgtt ggaaatatcc
    42721 ggataatttt tcacaagttt ttaaaaaatc aacgaaaaaa actttaaatt caactgcttg
    42781 aaattaaaac tggagttacg atgaagaaaa ttggtttgat tatgagtctg ttaatcgggt
    42841 ttagtttatg cggtcgccaa gccggaattc actttgaaaa gatggatttt gataccgccc
    42901 ggcagaaggc tcagaacgaa caaaaattac ttttgacgta tttctataca gattggtgag
    42961 gcgcctgcag gcagttgaac cagttggttt acacgaataa agaagtcggc gaatatatca
    43021 atgcccatta catcagtatt aaaataaacg gcgatagttt ggaaggcaag caactacgcg
    43081 agaaatacaa atatccaggc tatcccacga ctattattat gacctcggat ggagaggaaa
    43141 tcgatcggat tgtcggcttt gatggccaga aagatgaata tttccaattg ttgaaagatt
    43201 acaccgcagg acggggaact ctgggcgatt atctgaagaa attagacgaa gaaccagata
    43261 atttcgaatt gaattatgcg gtcctgaata aatattacga tcgcaacgat actccgatga
    43321 ttcggaagta tgcgcagatt gttctggagc ttgacccgga aaataccaaa ggtaagaaga
    43381 cggaaatgct ttatcgtctt gcttttgccg aatataaaac ttccggtgaa attaaccctc
    43441 tgatgaactt tattaatacc tgtaatgatg aacaatggtt agaacaggct tataacgaag
    43501 ctgtccgtta ttaccagaaa aattcagatc aatctcaagt tttagcaact tatgaatccg
    43561 caatacagcg acttccgcaa tcaacttcaa tgatgaattc ttatgcctgg tacatttttc
    43621 agaataaaat tgcagatcat tatgcccgag gaatcgagat agccagacga gcggttgaac
    43681 tgtcacctga cgccgaccat atctgggaca cacttggtca attgttattt gcggctgggc
    43741 gggttgatga agctatccag gctatgcaaa aagccagcga tctcaatccg caggaaaagt
    43801 catatcagga attgttgata aagtacaaaa aggtgctgaa tagttgattg gtctatagtt
    43861 tggattttga caattattga agacaataaa atattttgcg cattattatt ttaatagtaa
    43921 gtctgttttg gaagtttttt taccaattct tttgggcgat tttaatttat ctaccgtttt
    43981 aatagagttt tagaaaatct atgccatata aaactgtttt cgatgcctac catttatatc
    44041 atctcccgca attcgatccg gttattgacc tcttgttaaa ggataatcgc tttgaggttt
    44101 ttctgacgac ctcaagtgct aatttgccag atgaacagaa cctgacaaaa cacatatttc
    44161 agcggcgcaa tgtgcactgc attttcgcta ataatgaaga agaacgcgcc gctaagattc
    44221 gatctcttaa tccggatgta ttcatctgtg gttggtcgcg ttatccaatc gagaaattcg
    44281 ttacagaaaa aactctggtc ggtatgatct atcacggtat tggagttaaa ccatcctatt
    44341 ggcaagataa ccatccgcga ttgaatatcc gctttgtcga gggtcccttt cgtatacgac
    44401 aactgcgaga aaaaggaata cagactgatt tggaactgac cggctttccc aaaattgatc
    44461 cactattcaa tggccagatt gccagccggg aggaaatttt acgtaatctt gggctggacc
    44521 ttaataagaa gaccctatta ttaagccggc ataagatatt agtacgatat gtagttataa
    44581 attgttatat tacaggcatg gaaatagaaa aacaagacgc gagaaaatta tcaaaagaga
    44641 atctggagat gctgcgaaat caggcaatcc gactcttaaa acaggggcga aaacaagtag
    44701 aagttgccga gatactgggt gtccgcgctg caagtatctc ggattggtgg aagatttatc
    44761 gaacacatgg taaaaaagga cttatacttc gcaatcctgg tccggcaaaa ggtacaaatc
    44821 gtatgctcag ccaagatcag gagaaagaga ttcagaaact gattatagat aagacccctg
    44881 atcagttaaa gctgcctttt gcactatggg acaggaaggc aatccgagag ttgataatca
    44941 atctatacgg aattaaagta gctatccgta caatgggaga atatctgcac cgttggaatt
    45001 tcacaccaca gagaccatta aagcgtgctt atgaacaacg gccgcaagaa gtaaagagat
    45061 ggctggctga agaatatccc gtgatcaggc acagagcaaa gcaggaaggc gcggagattc
    45121 aatggggtga tgagacagga atgcgaaacg atagccacta tggcagaggc tattctccca
    45181 agggcaatac acctgtcatt aacctgtcgg caaagcgagt cagtataaat atgatatcaa
    45241 gcatcaccaa tcggggcaaa gtccggttta tgatgtatag aaagaacatg aatgctgatg
    45301 tgttgattaa gtttctgaaa aggttagtca aggggagtaa tcgaaagata ttcctgatcc
    45361 tggataatct gagagtacac catgcttatg tagtccgcga ctggctggag gagagaaagg
    45421 atcaaataga agtcttctat cttcctgcct attcgccgga aatcaatccg gatgaatatt
    45481 taaattgcga tctaaaaacc agtgtccact cacagtctcc ggcaagaact caggatgatt
    45541 tgacaagcaa ggtgacttct tttatgcgta aacttcagaa aagcccatcg cgtgtgtcaa
    45601 attattttaa acatccaaaa atcgcttacg cagcatgaat gttacgttta atgattgccg
    45661 gattaatata tgctccgacc ttttatccca gtagttttga atcatttgga ttgaaattgc
    45721 cggaaatggc taaagattta aacctgataa tcaaattgca tcaatggtct tattttttgg
    45781 aaaaatttag tggggtcaat ctacgccgtc atattaaact agccaacaaa atcaggaaga
    45841 aatatccccg agtggtgatg atcgagccag aacactataa tattattgac ctttatcagg
    45901 catctgatat tttattaacg gaagcctctt cgactattta tgagtttatg gctttacgca
    45961 aacatgtaat cgtttgtgat tttttcaaga aaaagattga acaccatctc ctaccaaaac
    46021 gaatttattt gcatcgtctc gatcccgaaa tgcacggaat tatgaccgaa ttttgctatc
    46081 atatcaaaaa accgcaagaa ttaactgtag ctcttgataa atgttttcaa aaacctgacc
    46141 cgttcgtcga attacgtgaa aaatacatcg cagatatgct ttataaatta gatggcagag
    46201 cctctgaaag aattcggaat tcagtattaa gtagagtaaa ttaaatttaa ttaataatat
    46261 gaatcaggag tatttatgaa agtgataata ttagcggcgg gaaatgccat tcggttagct
    46321 tctctcgcta atggaagtcc taagtgtctt ttgccgcttg gtaatacgac aattttaggt
    46381 aaacaattac acaatctgga aaaagtcggg ttaacggatg taattctctc ggttggttat
    46441 aaagtcgaaa aagtcgaaga gtatctgaaa aactttcgga ctaatctgaa aatccatctc
    46501 gaattcaatc cgttttatga catttccaat aatatcattt cactctggaa cgtccgtcac
    46561 ttggtatgga atcagccaat tattatcatt aatggcgata atgtatttga ttatcaaata
    46621 ctttcgcttt tactggaatc accttttgaa aatgttctga tgatccaaaa aaaagatgaa
    46681 tatgacggtg atgatatgaa ggtcaaagcc gaaaatgatc ggcttaaggc tattaataag
    46741 actatgaaaa gttcagaagc caacggtgaa tctatcggaa taatgaaatt ttcggccgaa
    46801 ggttgtgctc tgctgatgca aaaaatagag gaaatgactc gcaacaagga aaatttgcag
    46861 gtatggtatt tacgatcaat ccaggaattg ataaatgatg gcttaccggt tcacgtttgc
    46921 cgaataaacg gattgaaatg ggaagaagtg gattttccgg aagattattt gcgcgtaaaa
    46981 aatatgaatt gggaaggtaa ttgatcggga aaaatatgcc aggtaaaata aaattggatg
    47041 acaaatatct ggaaaaatcg ctaagactgc ttgaacgcgc cacaacactt ctggagaagt
    47101 ataatattcc atactggctg gaaggtggaa ctctattagg aattatcaga gaaaatagac
    47161 tgcttccctg ggataacgac ctggatattt ccatccgttc cgaagacttt gatcgtttgc
    47221 ggcgtattct gcctaagttc ttctaccagg gaatgatcgc taaagtcaga gggcacaaaa
    47281 tcgatgatcc cccattccaa aaaggagagg tccggctaat aaaggtttat gccaccaaat
    47341 atctattttt caagactccg ctcgttctgg acatcttcgt taagaaaaaa ttagatgatc
    47401 aatactattg ggtggttgga gtaaagcgac gggcaaagaa agcggttccg gctcgttttt
    47461 atgatgaact gacaaccgtt cagtttaata ataaaactta ttcgattccg aaattgactg
    47521 atgaatatct gacttatcgt tatggtgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    47581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    47641 nnnnnnnnat atagaaatag gataataact aaataggaaa ataagttaat gaaagccgta
    47701 atacttgccg ccggagccgc gcgccgctta gcacctatga ccaactcgat tccgaaatgt
    47761 ctgattaaaa ttggagataa atcaatactt gaaaaccagc tggatgcctt gcaattctat
    47821 ggaatcgaag atgtcttgat agtcgttggt tatctcaaag agcagatcat cgagaaaatc
    47881 ggtaaccagt ataaaacaat aaatatttcc tatatcgaaa acccgctttt ttcttcgacg
    47941 aatacggttt actcgctctg gctggcgcgc gcatattttg ccggtcaaga ttttttttat
    48001 ttcaatgctg atgtgctttt tcactacagt ttaatcggga agctattaga agcgccttac
    48061 gaatctgcta tgggtgtgga agtcaagcaa tgcggtgaag aagaagtaaa agtcatcgtt
    48121 gacgataaac tgcgtatttg gcggatcggt aagaaactca atctagccga ttgtctcggt
    48181 gaatttgtgg gcgttgccaa attcggtgcg tctttgacag ccgacttttc caatgctttg
    48241 aaggcggtaa ttggtgaagg tctgcagatg tcatttttcg aagccgctgt cgatcggatt
    48301 ctcgatcagc acgctttgta ttcggttgat ataagcaaaa taccagtcat tgaaattgat
    48361 ttcccggaag acctcgaacg cgctcgccag caaatctatc cgaaaatcat cagttttaat
    48421 gattaaccgc aaaactcgtt tatcatactt agtgtttcaa acgaaaggta ttaaaatggt
    48481 gaagggtaat ttataatgac gacggttctc tttcaccttc atcatcttta ttatctgcca
    48541 cagtttttac cggtggcgca ggcgatggag cgtgatggtg attttgaagt gttttactca
    48601 acggtaatag ataaaagtcg taatgattat gaatggtcgc gaaaaattat cggtgatttg
    48661 ggcggaaatt ttattacagt tgatagtgaa aaagagcgtg tcacaaaaat aattaagcaa
    48721 aatttcgatg ttgtggtttt cggcaaaagc cgcggtgcag aaaagttttg ccagccttca
    48781 tctctggcag ttttgctata tcacggaatc ggagtaaaag cctgttacta tactgactac
    48841 aatccacgga tagatgtgcg ctatgtcgag agtcaatatc gcctggaaga actgcgccga
    48901 cggaatatca agactgagtt ggtcgtaacc ggtttcccca aattggaccc actgatagac
    48961 agttctgtta gagattatta tttcgacaag tatcatttgg cgaaaagcaa accgacgatt
    49021 ttatatgccc cgacctttta tccgagttcg attgaagttt ttggcgaatc gttggcagaa
    49081 cagactaagg attttaacct cattgtcaaa ttgcatcatt tttcctggtt aatgaaaaaa
    49141 tatcaacatc agcgcaaact ctggcaaacc ctggtgcaaa agtatggaca tgtccgtttg
    49201 gccccggtcg aggaatacaa tatagtgccg cttttttcgt gcagtgatat tttagtaaca
    49261 gaagcgtcgt caacggcttt tgaatatctc gccaccagcc gccccataat catcgctgat
    49321 tttgttaaat tgcgctggaa gcaccgcctt tttcgaaaaa atttcgaacg gcaacgtctt
    49381 gactcggcga taattgacga actcgatttt gcttatcacc tcgaacagaa gcaagatttg
    49441 tacgaagttc tgaatcgtgc aataaatgac gttgagggga agagacactt gctgaaccgg
    49501 aaacgggatc agtttctggg aaatgttgat ggcaaggcat ccgagcgagt agtcgctgat
    49561 ttgaaaaatc gtctgaataa gtaaaaatgc gctatttatt tttcatcacc aagccgtatt
    49621 caattagtgt tattcgacca ttgtatgagg aaattttccg cggagcgtgg ggggaggctt
    49681 taatatttgc tacaccgccg gtcaaagcct tgattgattt tgcggcgcct tcaactgatt
    49741 cgattcaagc ggcgatagat ttccagccgg aagttgtttt cgtgccgggc aatttcgttc
    49801 acgataaaat tcccggttta aaggtgcaaa tttttcacgg actatgtgaa gaaaagggcg
    49861 ggcattataa aattactggt ttcttcgatc tctactgtac cagtggcccc ttaattactg
    49921 aaaaattttt gcaattgtca caaaagtatg gtttttttaa agtaaaggaa accggctggc
    49981 cgaaagttga tggtttgcta aagccatata atcgaaatga aatttgtcaa aatctgcgga
    50041 ttgacccgca gagcaaaata attctatacg ctccgacttt tagcccgaaa ttcaaatcct
    50101 caaacgaaat actaaaagtt ctcaatgata taccccgtac tggtgaacag tggatcatta
    50161 agtttcacga tctaatggct tccgaagacc gccggtgttt tgaaaaattg ccccgggata
    50221 aattccggat ctatactggc tacgataata ctccgttatt gcaagtcgcc gatgttttga
    50281 tctcggatac ctcgtcaata gtgtatgaat ttatgctgtt ggataaaccg gtggtaacaa
    50341 tcggagcgaa agtcaggcaa gaaaaagcac ttaacattga atcagcgtca gatttgcggg
    50401 cggcggtcga gcgttcactg aaaaatccgc aggaattcgc tgaaaatcgg cgccgaacgc
    50461 tggcgcagat tcatccttac attgatacat taaatagtca gcgtgttcta caagcggtag
    50521 aaggattttt gagcggcatt actcccggag aattgaagaa aaagccgtta aatctttatc
    50581 gtaaatataa agtgcgcaga cagttagggg gctggttttg aaatgtgtta tcgagcgtta
    50641 catggcgaag agcttactcc agaacagctg aagcatttgt gcgatcgtaa ttggattcgc
    50701 aacaacgctc gtcatattta caaagataag gtcaatatcc tagcggaaat cgaagtggaa
    50761 ataaacggag tcccgcaggc agtcgtgatt aaattatttg gctggcgcaa ccggatttcc
    50821 tgctggttaa gtccctttat gcgttcacgg gctaagaaat cctgggacgc ttcatttagt
    50881 ttgctaaaat gtggggtcaa agttcccaag ccgcttacgg tttatactca gcgtcgtttt
    50941 ggatttatca aatgcaattt tttgttaacc gagaaaatag gaaattttac cctggcgcga
    51001 aattggttaa gagacccgag cgctaaaccg tccgataaaa gaattatcgt tgaaactctt
    51061 gctgaaatgg tcaggaaaat gcaccaagcc ggtatggtgc attcagattt gacgccaggg
    51121 aattttctca tcaataatac taatccgtct gagatttatc tgattgatct taatcgtcta
    51181 aaacataaaa tatttctgac tcggtcgtta aaaatgtatg atattgccaa attgaatttg
    51241 tgtcagtgtg gctttgctga ggaacattcc gattgcttat ggatattatt ccttgaaaaa
    51301 tatgataaag aagagtttga gaaaaataaa ttagccctgc gtttggcgat gcgtcgtctt
    51361 aaaaatcatc gagctatgaa atctttacgg aaatcatcaa aaccgaaaaa tttaccgaca
    51421 ccgtgaaaat aaggttttct aaaaaattaa tttttatcgg ctggattgga ttaactaacc
    51481 tactatttgc ccaaaatcga attatcagcc gctttccgat tggtgaggaa ttgacttact
    51541 ccgcctggtt aaattttatg aaggtcggtt attcgactat aactatcaaa gccatcgaat
    51601 tggtagatgg atttccggtg tatcacgttg aatcaataac caaaacaagt cccttttaca
    51661 gtagaattta tcgggtgaac gatcggttta attcctggat tgatctggat ggtctctttt
    51721 cgcggcgtta tgaaaaaagc ttgcgggaag gaaaatataa aaaggattac caggcttatt
    51781 ttgattataa tgagaaaaaa gcctatacct cgcgtgatac gttggatatt gacggtcgaa
    51841 tgcatgatgc cctgtcggta atattttggg tgcgggcgga atatttattt cctggtcggg
    51901 ttattcgact taataatttc gacaataata aattgcgact ttttaatgtc atcgtcagtg
    51961 gtctcgaaac attgcaagtt ccggctggcg aattccagtg ttttgtatta gagccatatg
    52021 ctgatgatgg gaaattattc aaaaatcaga ataaaatgac aatttatctt agtaatgact
    52081 ccttgcgttt accggtgatg attgataacg aagctaattt tgggcgaatg atttttaaac
    52141 tcgagaaccg gacaattcgc caatagtagg ttcgcagaaa accgcaatca ttttcaaggt
    52201 cataattttt aatgaaatta gaataaaatt ttcgaccact tacctttacc tattttcttt
    52261 ttttctctat cctaaaactc aaacttaatc aatggatatc ggtagatgaa aaatgatgct
    52321 cggtttctga aagtcactca agaatttatg gttagattgg ttcttataca caacttatac
    52381 aaggatttta attttacatt agacacacgg cttatgatcc cgccaggcgg gacttaccac
    52441 tgagctggag ttgggttatt catagaacta aataatcgga gaaaaactta ttagcattga
    52501 actttagcct aactgcttcc ttagtagcgg tggtgggatt tgaacctacg acacacggct
    52561 tatgaagccg ctgctctgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    52621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt
    52681 agcggtggtg ggatttgaac ctacgacaca cggcttatga agccgctgct ctgaccactg
    52741 agctacaccg ccgaaaaaaa gcggtcaaat ttacgtacta tggtttaagg atacaatatc
    52801 agtggattct tggtggcttt attttttttc attacattca tttttattaa ttgcgagact
    52861 taatgaaaga aaccctacta attcagaatc ccctaatgtg tgcgccaatg actggtgaaa
    52921 gaagttcgat gaaaaatatt aaaacctttt caggcggtca tatctttgtt gagaatggaa
    52981 aaattaaatc aatcggtccg caggaattta atggtaaagc cgatcgagtg attgatgctt
    53041 cacggatggt agtcttaccg ggattgatca atacgcacca tcattttttt caaactctaa
    53101 ctcgtaatat cttccctacc gaaaatgcgc cattatttga atggctaaaa actaattata
    53161 ctatttggga tggaattact gagtcggcta tagaaatcag tgcagaggtc gcaatcgccg
    53221 agttactcaa aaccggttgt acaacgacat cggatcatct ttatcttttt ccagcccagg
    53281 ctgatgctaa tctgctggat atgacgatta atgtcgcgcg tgaaatgggg atccgttttc
    53341 agccaacgcg cggttcaatg tcgcttggtc aatcgagagg tggtttacca cctgacaata
    53401 ttattcagaa agagagtgag atcgtagctg acattcgtcg gcttataaaa ttatatcatg
    53461 atgattcatc gggggcgatg acacggattg cccttgcgcc atgttcacct ttttcggtta
    53521 cgattgagct gatgcaaatg gtagtaaaat tagccgatga atttaattta caaatacata
    53581 ctcatttggc tgagacgctc gatgaggaaa agttttgtat agaaaattac ggcttgaggc
    53641 ctttcgcttt actgaaaaat ctggggtgga ttcgacccaa tgcctggttt gcgcattcga
    53701 tttatttgaa cgagtctgaa atcgccgaaa tgggggtggt tggatgtgga gtaagccatt
    53761 gtccaacgtc aaatatgcga cttggatcgg gaattgctca aattcgtgag atgctagatg
    53821 ccggagtcaa tgttagcctc ggcgtagatg gttcggcatc gaatgactct tcgaatatgc
    53881 ttttagaatt gcggaatgct atgctggttt ctcgattacg gcaaaagggc gaatggttga
    53941 cgactgacga aatattatgg atggcgactc gcggtggtgc agcggtactg ggacgcgatg
    54001 acatcggtca attagctgaa gggaagtgcg ctgatctctt tctggtttca attgatcgga
    54061 tagaattagc cggtgctcag catgatctgc tggcggcatt gatattcgca gttctactct
    54121 cgcctgttga ttacgtcata gtcaatggtc ggcttataat tgaagaaagc caattaatga
    54181 ccgctgatga acagaacttg attaaatgtc atcaacgtat ttctgatgag ctgattatgc
    54241 gagccgaacg taaaactggg aaaaaattcc gtcgctaaaa tataaaaata atccttttca
    54301 aaattatata ggagattaat atgaagcaat ctgttgttcg taaaattttg gtgatagttg
    54361 tttggattag ctttattcca ttagttggga tagcgcgcga aaaagccgac tgggaaggtg
    54421 gctatccgga cggttgtact tcagttatgg ttgggaaaaa agcctcccgc gatggttccg
    54481 ttatgaccag tcacacttgc gatagtcatc gcacacgctc ttcactcgat attgttccaa
    54541 ccaaaaaata tagcaagggt gaaatggtta caatgctaaa gaggattgat tgtgattctt
    54601 tggcgatgcc tgcctatcaa tatctggcga tcggtgaaat cccgcaagtt gtcagtactt
    54661 atggatttat caatacggcc tacccttgta tgaatgaaaa acagctagca atcggcgaat
    54721 ctacttttgg tggtcgcgag tgtttacgtt cggataacgg cttgattgac tgccagcaat
    54781 tggtgcgtct aatggtagaa cgctgttcga cagctcgcga agcgatccgt atggcgggtg
    54841 aattaactaa gaaatacggc tggattgacg agggagaatg tttgacaatt gccgataaaa
    54901 atgaagtctg ggcactggaa atcgtcggac cgggaaaggg taattgtggt tcaatctggg
    54961 tggctcaacg cgttccggat gatcacgttt cggtgaatgc aaatggtagt cgcatccgtc
    55021 agatagacct gaaaaagccc gattatttta tggcttctga aaatgtcttt aaggtggcac
    55081 aggatagcgg gtggtggaat ccgaaagaaa gtccattcga atttgcttat gcctatgcgc
    55141 ccgaaagccg tcaaacgttt tcggcgcgaa ggcgtgaatg gcgtgtatta gatttagtag
    55201 cgccatctct aaaactacac ccagacgcag aaaattttcc attttccgtt aaaccggata
    55261 ctctggttga cttggctaaa atgattagaa tattccagga ttacttcgag ggtacggatt
    55321 ataatttcgt taaaaatatt acctggacta actcagaagg gaagaccgaa atctcaccat
    55381 ttgcaaatcc atttatgcct tacgatatga atcacatttt caaaatcaac ggcggttggg
    55441 gttggcgtgg tgaacgcccg atcgctcgtt ggtatacgat gtatgctacg ataactcaat
    55501 cgcgggaatg gcttccagat gagattggcg gagtggtttg gctagctctg gataatgtcg
    55561 ctacctcgat atatattcca ctatattgca gtatttatga tgtggcgcca ccctataagg
    55621 ttgacggacg aacgaaaggc tttaatcggg aatccgcctg gtgggctttt aatcggctgg
    55681 gaactctaac ggctcagcgt tggggagata tgcgctatga tgtagcggct gtgtggggac
    55741 caatgcaatc cgaattatta agacggcaga acgaaatcga gcgtcaggct ttggggcaat
    55801 atagacatag cccggatgcc gcccgccatt ttctgactaa atatagcatt gactgggggg
    55861 ctaaagttgt taaccgcgcc tggaaattgg gggatgaact ctggaccaaa tacgatgaac
    55921 agttttgaat ttttaccaca aaggctcaaa ggcactatga tttcttttta ttattttaag
    55981 aagaatgaaa tttgataggt caacaaataa atcttaggtt atgccgccag aaggatgact
    56041 agtttaaaag aattttccaa atgtttgcta cttttttctt gaccttgcca gggtaataga
    56101 caaatcggtt cggctcctta ccaagcccga taaatatacg gtaaattttt agtgtcttag
    56161 tgccttcgtg gtaattcttc tacttgccat cagccgggat tggccataga aatcggccgt
    56221 tttcaagcaa atgctgaatc tgagtaatac tctgagcggc tttgcgttcg actgattcgt
    56281 aggtattgaa agcgttatga ggagtcagaa tgacatttgg taatttagca agagcaagag
    56341 tagcctggac ttctttgtta gggcttgtac ttccgcttcg caaagcgaca gccaattcac
    56401 tttcaacatt atagacatct agaccgacgc cgccgagatg gccttcctgc ataagtttta
    56461 ataattcggt tgaaggtgac tgctcgccgc gagcgatatt gacgaaaacc acacctggtt
    56521 tagccttacg aagtcggtcg gcattgaaat agttatgatt ttcggaggtt aagttcatcg
    56581 cacaaatgat tatatcggct ttcggtaaac cctcctcgat actgatataa ttaacatcgg
    56641 agtgtttttt aacaatatca actcccagaa cctgcattcc taatccaatt ccaattttta
    56701 caacttcgta gccgatattt ccgacaccaa cgaccagaag tgttttcccg gcgcattctc
    56761 gaccagttaa tccgtcgcgg tggaattttt gaaactgctg gagttgctgg ggaagtttgc
    56821 gcagaagcgc cagagcgatg agaatagcct gttctgctac ggaacggtta caataaagcg
    56881 gcaaatatcc gacctgtagt cttgaaccgg ctttttgagc taacggtatg aggtgatcgt
    56941 agccggtact ccggcttaaa attgccgata attccggtaa ccaggagact ggcacaattg
    57001 attgcgttcg gatactaatt attttagcgg gtggtgcagg atgctcactt tcctgaatgg
    57061 ttcgccaatc aaatccagcg gtgatatttt caggcagaaa gcgctcgatc agctcggctt
    57121 cttcttgaaa tgcttcgtag aaaaatacgt caatcataaa aatccctgtt cctaattaat
    57181 ctatttaatg atgtttaaaa cactagttta gtgaaattta aaattccgtt tcgattagct
    57241 gatatttgcg acagccgagc ccgatttttt caccgtaatc gagctgaatg ctatagtcaa
    57301 tttcgggatg ggctaattgg gcaatattct ggtcgtttac ctggttggtc agatcaatga
    57361 cggcttgatc aattgctacg ggatcgaagc cagccagaac gccaatatcg ggggcaatcg
    57421 ggacttgttt cccaggcagg cagtcacagt ctttggtcat attagttaga aaggttaggt
    57481 agccaatccg accgtttttt tgccgaacaa ttccaagagc atgttccgca atctgttgtt
    57541 gtaaacgcgt gctactatta ttccatttaa aatggacagc atcaaaccga cagacggtca
    57601 aacactcacc gcaaccgata caaatatttt ggtcgataat agcgaatttg tcaggaatct
    57661 cgatagcatt tgctggacac catttcatgc aaaggccaca agcggtgcat ttattggatt
    57721 taattatggg cttactgacg gaatgttgag ctaatttgcc tctacgactg gacatcccca
    57781 tgccgaggtt tttgatggtg gcgcccagac cggtgctcaa atgaccggtg acgtgagtaa
    57841 tggcaatgat agaatttgcc gctaccagtt cggtcgctaa cgacacggtt ttgttcagag
    57901 gtgcactgac ctcgatctcg gtctcgttcc ggccgataat tccgtcggcg ataataaccg
    57961 gtgccccgac tttttcatag gtaaatccgt tatcgtaagc cagctgaaga tgatcaatgg
    58021 cattctgacg cacactttta tacagcactg tggtatcggt caaaaatgtc cgagcgccgc
    58081 aactctttat cattcggaca attggttcga cataggcggc tggaacatga gtaacgttgc
    58141 ctttctcacc gaagtgaatt tttatcgcgg taatgtcgcc cgcttggatt actctatcca
    58201 gaccggcact tttaaacaat tttttcagac gattttttgt ctgatctacc ggctcgtcgg
    58261 atttgaaacg cataagctga atctgattaa caggcatatt ttttctccat ttgggcggaa
    58321 tttaatagcc agctggtcaa ttggcaattt gaaaaatctt aaaattgaaa taaatctctg
    58381 tagaattatt tgtagccgtg tatttaacaa ttaccggatt aattgccatg agaaatattt
    58441 tataaagaat tggataatag ttaaattatt aataaagtcg agcgttattt caggagaaga
    58501 atcaatgaat tctggattat ttattcccaa aaattatcgg acaattctaa caacagtgga
    58561 gaccgaaaag gcgattctgg cgctgaaaga tttttttcag cagaatctgg cttttgagct
    58621 ggatttgatc cgggtcactg caccgctgtt cgttaaagcc ggcaccggta taaacgatga
    58681 tcttaatggg gtcgagcaac cagtatcctt cactgttaag gagatggcaa atacccaagt
    58741 cgaaatcgtt cagtctttag ctaaatggaa gcggatgatg ctgacnnnnn nnnnnnnnnn
    58801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    58861 nnnnnnnnnn nnnnnnnnnn nnnnntgtaa aaaattcggt gtcatttttc tgattggtat
    58921 cggcggacag ttacagaatg gcgagcctca tgacggacgt gcaccggatt acgatgattg
    58981 gtcaaccccg actgtcaatg gttatcatgg gctaaacggc gatattatcg tatacaaccg
    59041 agttttggat tgcgcttttg aattgtcctc gatgggaatc cgggttgata gagaagctct
    59101 tctccgccag ctgaaaattc gccaggttga agaacgtcag aatctttatt ttcatcagcg
    59161 tctattaaaa ggcgaattac ctttgtcgat cggcggtggc atcgggcaat ctcggttatg
    59221 tatgttttat ttacggaaag ctcatatcgg ggaaatacaa tgcagtatct ggcctgaaga
    59281 aatggtcaaa ttatgtcaga ggaacaatat ttttctgtta tagctgtttg tattttttca
    59341 accaagcccc ttgatattaa ttaaaccggt tcatactgga attcctcaat tttcgcctat
    59401 tggtatcggg tcaaaaacaa tctcttgggc tttcttatct caaagtgtaa attcttagtt
    59461 gtttattgaa ggacaaatga tggagtcatt atgaacacaa taagcaaata taaagtggga
    59521 ttaatcctgc ttggattggt attaactata ccgctctctg ccgccaaaaa aattaacttt
    59581 accgaagcta aagcccgcta ttctggtctc atgatattcc agaccaacga aggttcggga
    59641 atcggtggtt attttgaata ttcgcttaaa aatgccaatc gcttagccgc ccaggtcatg
    59701 tttacggttg ccaccggtga cgattatccg ctctattact atgattacta ttatgatcaa
    59761 tattattact acgaaagtgc cagcaagcgc cgtttatcat ttctgcattt attaggcgga
    59821 tataaaagaa tattatttgc ggacaaaatt gctaatgatt tccgtcctta tcttgaatgt
    59881 ctgggtggag cggttatggc actcgatccg ccgaatattc ccgattttag tgataggatt
    59941 aagaaaatga ccacagcctg ggcgccgggt ttccagattg gaggtggcat tgatttcctt
    60001 tccgggccta aaacgctcgt ttcgatcttc gccggttacg attatcttaa gttcgctcaa
    60061 aaaatagatc caccgcaggt ctattatgac gaggacggta accctttaga aaacagatac
    60121 tccggtcaaa aggatttcag tggattggtg atcaaaatta gttttggtaa aaagttataa
    60181 tgcacgctta ccagttctat atttcaatgg agaaaattca ggaaggaaca ttcgacctcg
    60241 agggtgatga ataccgacat tgttgtcgcg tcctccgcaa aattaggggc gattctattg
    60301 acgtcttcgc aggcaacggt cgtcgctggt cagcacgaat cgaaaagatt agcaataata
    60361 aagctgaatg tcgcataatg gaagagtatc cacctcaatc gcggttgaag ccggaaatca
    60421 ttctcggagt tggcttattg aaaaataatt taatggatga tgtagttgtt tctgctacag
    60481 ccttgggtgt cgctgggata atgccacttt ataccactca ttcgataaaa aataaaatca
    60541 accacgaacg gctggcgaaa ttagcactat cggctttgaa acaatccgga ctggcttttt
    60601 tacccataat ttcggattgt ttgacgctgg ataaatggtt taatctgacg gaagaaatcc
    60661 ctctcaagct gattgccgaa caacacgctt cggccgactt gaatcgtatt cagcaattac
    60721 aaacggttga gcaagtagcg ttaatgattg gtccggaagg cggattcgaa gaaaacgaaa
    60781 tagaaaatgc tcagcggttt ggtttcaata aagtaaatat ttttccgtat cgtctgcgaa
    60841 ccgagttagc ggtcgtaacg gctctcgccg gaatccggac gctgacgtta aaaaatgagg
    60901 cttataatgg aaactgattg cattttctgt aaaattgccg ccgggaaact cggcacggag
    60961 tttttatatg aatcggatga aatagtagct tttcgagaca ttcatcccaa agctcccacc
    61021 cacatattga taattccgcg ccgccatatt cccaggataa ctgacttgca ggaatccgat
    61081 gaaaagttag tcggacgaat ggtcacggtc gcaaatattc tggcggaaaa ggaagggctc
    61141 aaagaaagcg gattccgtct gatatttaac tgcggacgtg atagtggtca ggaagttttc
    61201 catatccact tacatttact gggcggacgt ccatttcact ggcctccggg ttaaggaaaa
    61261 gcgtaattgt acatttctaa actcacactt tacggtttta aatcatttct caaaaaatcc
    61321 gaaatccaat tcggacgagg gattacttca atcgttggac cgaacggttg cggtaaaacc
    61381 aatatcgtgg atgcaatccg ctgggttatt ggtgaacaga aagccagcat tttgcgcgcc
    61441 gaacgcgtca cggatgtaat tttcaatgga accgctaccc gccgaccgct gaatttagcc
    61501 gaagtaagtt tgacaattaa taatgttagt ggacgagtac cgatcgcata ttccgatgtc
    61561 gaaattacgc ggcgattata ccggaacggt gaaagtgaat attttatcaa tcgcaatctt
    61621 tgtcgtctga aagacattac cgatctattt attgataccg gtatgggggc gaatgcctat
    61681 tcaataatcg aactcaaaat gatcgagaat attctcagtg aaacgccgga agagcgcaag
    61741 cgtctcttcg aggaagccgc cggtgtcaat aaataccgcg tccagcgtaa agccgccttg
    61801 cgtaaactcg aatcaactcg cgacgactta ctccgtctga atgatattat caatgaagtt
    61861 gatcaaaatg ttaaaaattt gaaaaggcaa attgcgcgct atgataaata tcaggaaacc
    61921 acccagaaat taatcgaagc cgaggtaatc ttagccagtc gccgcatctt cgacttacgc
    61981 gcaaaacagg cgccgatttt aaccgatatt aacgctaaac aaaatttact ggaaaaaatt
    62041 agcgccgaac tgaatgccaa agaatcacaa ggacgcgaac aacaaaaaat tctaaataca
    62101 atcgaaatcg aattacagaa taagcgtaat gaactcgaga ctttgcgggc taataaagcc
    62161 aaattggaag ccgccggact acttctcaaa gagcaacttc gtaacaatac ccaaaacagc
    62221 gctcgcctaa ccgccacgat taatgcttta cagaaaagta tcgaatcagc ggaaacccgc
    62281 cagaaaataa tcgaacagga tttggctacc aacgcccaaa cccttgaaga aaagcgtcag
    62341 gtttacaagg cggcagtcga agcccagcag gaaatcgaaa accattataa aaaattaagt
    62401 gcggatttgc aggcacttca ggacgagcgc tttaatgcct tccggcaaca ggccgagcaa
    62461 actgcccatc taaataatct taaagaaaat atcaatcagc gcgaaaaaga attacaaaaa
    62521 gttatcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    62581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggtc gccaagcccg
    62641 actgaatgaa ttacaagaag aaattaaatc ggttgaaaaa caaagaaccg ctctgctcga
    62701 acagctccaa acagctcagg ctgaaattcg tcgccacagt gaaatgcgtg aaaaactgta
    62761 tcgcgatcgt cagcagatag aaactgaaat tcgccaattg accgatgtct tgcgggctac
    62821 ggaatcacaa caagtgcagt cggctggggc tctggcggct cttaatgacc aaaaagtgat
    62881 gcttaccacc gcgattgcca atttcaaaga aaggctggct aaagaaactc ccacccaaac
    62941 taatgttgac agtcaattag cccagatgga tcaacttata tctattaaaa agaccgaact
    63001 cgaccaggta cgtgccaacc tcgatgggca taatcaaaat ttgcaaaacc tgcgagttga
    63061 gctgattggt ctcgaaaata aagcccgcaa tttatctgac gaaaaggtgg ctttagaacg
    63121 aaacctgaag aatagtcttg aacaagttga aaaagcccaa caagaactcg ccgctttaga
    63181 attggaaaag agtcaactgg aaaaaaattt ggcagaaaat aaactcgccg atgccgaggc
    63241 agtaactctg gtgaatcaaa ccgaatctaa ttacaaccag attggacaag agtttcaggc
    63301 actacggcag acgattgaag cgattaatac cgaaattata aatttgcgtc ataccaaaga
    63361 acaaaacagt gaggagctga aacgtctgga actggctagt tctcagttta ttgctaatat
    63421 caatgaaatc agctccgtcc tctgggaaaa atacaatcgg acgccattag aagaaatacc
    63481 gcccgatttg cccactgaaa cggaagcccg ccaggttgtc gaacgttacc gacacaattt
    63541 ggagcaaatc ggcatggtaa atatggcagt caaggaagag tatgaacagg aatacacacg
    63601 ctggaaattt ctaagtgatc agcgtgatga cctagtatct tcggaaaaag gattgacaga
    63661 agtgattacg cagattgacc aggtcgcccg tgaacagtat ttggaaactt ttaatcaaat
    63721 ccggagcaat tttaaaacca ctttcgacat tttcttcggc ggcggcgaag ccgatctgcg
    63781 tctaattggt tcggatgatc ccctcgaagc cgagattgat atctgggctt gcccgggtgg
    63841 taagaaaatg cgctcactgc ggatgctttc tgccggtgag aaagccctca cggctatagc
    63901 tttactattt gcaatttatc aggtcaaacc cagtcctttt tgtattctgg atgaagttga
    63961 tgcgcctctt gacgatgaaa atacgcgtcg ctttaccagc gtcattcgaa ctttttcgga
    64021 aaatacccag tttataatcg ttactcataa taaggcgacg atggcgatcg ccgacgcttt
    64081 atacggagtc acgatggctg aaaaaggtgt ctcacaaatc gtttcggtta aattggagta
    64141 acctatgcgt atcaaaatat ttttaatgtt aatgtgtttc ttgattgtga actcatttgc
    64201 cgtagaattc gatgtagatt atgcggtctt caaaggcaat gaggaagtca gtgtagttga
    64261 ggtttatctc ctgattccac ggacgttatt tcaatttgtt cctaatgaaa atgaatattg
    64321 ttcctatgct cttattcgag ttgctctggt agagggagat tccgtgcgcg atattcaaga
    64381 gtggcagatt actgatcgcg ttgctgatac attacaacta gctgaaaatc agaaaattcc
    64441 ggaaatagct actttgcagg tcaaacccgg caattatcag ttgatcatag ttgtggcgga
    64501 tctgattaac cgacatcagt atcattatga taagcaaatc gagatttcag attattcgga
    64561 tgatcaatta aggctcagtg atattcagat tagtagccag atttctaaaa ccagtcaaga
    64621 aaacaaattc agtaaatatt tcggttatga tgtgattccg aatgccagtg ccatttttag
    64681 ccaaaggcaa aatttgattt attcttttgt cgaagtttat aacttgaaag ttgatcccgg
    64741 taaaccgtcg aattatctag taaagtatga cattgcggac ttaaatggaa aagtagtagt
    64801 aagtagtgat tggcgttctc gaaagaagcc gggcacttcg gcggtcgaaa tcaataatct
    64861 cagtattgct ggcttaccgg gcggtttgta tgatttaatt attgaagtaa atgatgaggc
    64921 gactcaaaag acagtgaaga agatcaaacg gttttacatt ctcgaaagca aatctcagct
    64981 tgccagtctc gaaatgaatg ccgaggctat ccagctggag ggcaaatctg aaaaagaact
    65041 tgatgaaatt tttggaccct tacgatacat agccaccgaa acggaaatta gacgctacaa
    65101 aaaatccgat gttgcaggta aaaagcaaat catcctccat ttctgggata atcgcgatcc
    65161 taatccccaa acaccaatta atgaaaataa agccgaattc gaacagcgtc tccaatatgt
    65221 caatcagcaa ttttctacca atcaagtccc cggttggaaa tcggattttg gacgggtttt
    65281 tatgatttac ggaatgccct ctgaaattga acgctttccc agtgcgcttg agagcaaacc
    65341 ctatcagatc tggtattata atgaaatcga aggcggcgtg aaattttatt tcgttgataa
    65401 aaatggattt ggtacttacg aactggttca ttcgactgct cgcaatgaat tgcaggatat
    65461 aaactggcaa cgctggatat cgccttattc caccagccca gatttaactt attaggaagg
    65521 taggtaaaag gatgagaatt ggaatagacc tcggcggcac aaaagtttcg attggattgg
    65581 ttgatcaaaa tcggcttatc ggtcaaccgg ttcgttttat gattgccgaa tgtaaagacg
    65641 ccgacgattt agttaaacat atgaaagacg ctattgaccg aagtcttaaa gctaacggga
    65701 tcgtctggca gaatctggaa atgatcggaa tcggtagtcc cggaccgctc gattataaaa
    65761 ctgggataat tttacacact cctaatctgg tgatgttgcg caattatcca ctcggaccaa
    65821 agctggaggc tttgaccggt ttaccggtgc tggtgaataa tgatgccaac tgttttgcgc
    65881 tcggagaaca acgcgccgga ggtggccgtg gtctcgaata tgttctcggt gtaactctgg
    65941 gtaccggatt tgggttggga ttcatttatc gtagcgaaat tttcaatgga gctaccggga
    66001 cggctttaga gtttgcgatg actccttatc ttgatggtgt ttacgaggat tatatttccg
    66061 gccgaggctt ggcgcgcatc tataaatctg tggttggaaa ggaaatcaaa ccggctgaag
    66121 ttaccgcttt agctaaaaaa ggcgattcag atgctctgga aacctggcga atattcggcc
    66181 atcatcttgc ccaagcgatg atagttctgg ttatggtgct cgatccggat attatcgtaa
    66241 ttggcggatc agtgtcccaa ggttgggatt ttttctatca aacgctggaa accgaactgc
    66301 gggcaggaat tcatgccggt ccagccggtc atctgaaaat tacccgcagt gaattaggcg
    66361 aactggcggc tattatcggc gcggctagtc tttaaaaaaa gctgatcgtt ttaattatgc
    66421 actttttcaa aactctcgtg actggtgtta cttctctgct atttccaccg atctgcaaag
    66481 gctgtgaaaa aaggatggat tcaaatgtag ggctgatttg tgcggattgc tggcaaaagt
    66541 tacaattgtt tccggcggaa gcgataaaaa ataaaattat tccggaaagt cttgatcagg
    66601 tctggccggt ttttctattt gatgaattgt ttcagaaaat aattcacgct ttgaaatatc
    66661 agggaaatgt ttcgattggt tacgagattg gacgacgaat ggctcttcat ttgccatctg
    66721 aaataaaaga cctccaatcg gcttgttacg ttccgattcc tttgcacccg ataaaattgc
    66781 gggaacgcgg atataatcaa tccgaagcga ttacacgcgg attggcgtcg aactcaggca
    66841 ttagagttga aaccaaatta cttaagcgaa tcaaaaatac ggcaactcaa accaaattaa
    66901 acgcagagga acgccaggct aatatggaaa tggctttcgc gattaatcaa aaatcttctg
    66961 tttcttcggg caacacgctt attttaattg atgatgtttt tactaccggt gctactctaa
    67021 attcagccgc gggagtacta cgccagtccg gttttcataa agtcatagcg gtaactgccg
    67081 ccgctccact ttagcaggaa tcaatatttt tccttggaat aattgctcta agaaattaaa
    67141 attgaaagtt attttaaaca atgaataaac taaaggtgaa atatgtcaag attgactgtt
    67201 gatgatttaa aagtaaaggg taaaagagtc attatgcggg ttgatttcaa tgtcccgctt
    67261 gatgaaaatt tgaatgttcg agatgatttt cgcattcggg cggctttacc gactattcaa
    67321 aagttactga atgacggcgc ccgtttgata ttgatgtcgc atctcggtcg tccaaaaggt
    67381 aaacccgtgc cggaattgag tcttaagccg gtcgccgaat gtcttcagaa atatttgaag
    67441 aacaaagtat attttgcgcc tgattgtgtc gggatcgaag tgaaaaactt agccaattcg
    67501 cttcaggatg gtgaagttct tctgctggag aatctgcgtt ttcatccaga agaggagaaa
    67561 aatgatcccg aattttctgc caagttagct gagttgggcg agatttacgt caacgatgct
    67621 ttcggtacaa gtcatcgagc tcatgcctcc accgccggta tcacgaacta tatcccagtg
    67681 cgggcggctg gttatttgct tgaaaaggag ttgcattatt tgcaggatat gcttgagaat
    67741 ccggagagac cgttcacggc tattttcggc ggagcaaaag tcagtgataa aattgatatt
    67801 atcgagcgtt tgtttggtaa agttgataat attttgattg gtggcgggat ggcatttacc
    67861 tttctaaaag ccaaaaatta tgaagtcggt aaatcattgc tggaggctga taaaatcgat
    67921 tttgcattgg cgactttgaa gaaagccgaa cagatgggat gtaaaatagt tttgccgacc
    67981 gatgtagtcg ttgcatctga aatcagtgat acggcggtgc cggaaatcgt ctccgtggat
    68041 caaattccac cagataaaat tggtgtcgat atcggtcctg agaccacgat gattttctgc
    68101 gagataattg aaaaaagcaa aacagtcctg tggaatggcc cgatgggcgt cttcgaagtc
    68161 aaacaatttt ctaccggtac agaatcaatc gcgcgcaaat tggcggaagt aacgactaaa
    68221 ggcgttatta ccgtcgtcgg tggtggcgat tcggcttccg cgatgaaaaa attcggacta
    68281 atggacaagg tttcccacgt ttcgacaggc ggtggtgctt cgctggaatt gttgagcgga
    68341 aaggaactaa ttccgattaa tcttatatca aagaagtagt tgattatgaa acaatatatt
    68401 gtagccggta actggaagat gcataaggac attcaggagt ctcgcatttt cgtgtcgaac
    68461 attctcaata agttgctgaa acacgaaaga gcaacggtta ttttatgtcc tccttttacg
    68521 gcgctttttg atgtttataa cttaataaaa aacacttcca tcgcgctggg cggccaaaat
    68581 atgcacagcg aacccaaggg tgcatttact ggcgaaattt ccggtgggat gctgatttcg
    68641 gccggctgtc aatatgtcat tttagggcat tcggaacggc gacatatttt cggcgaaacg
    68701 gatgacttta ttcgggataa agtcaaacaa gctttgcagg ttggtttgaa accgattatc
    68761 tgcgttggcg agaaattgga cgagcgtcaa tccggcgaaa ccctcgaggt tgtccgccga
    68821 caatgtgatt cagccctgag cgtcgtcaat gaacaggaaa taaagcgatg tgtgattgct
    68881 tatgaaccgg tctgggcgat tggaaccggt gttgtcgcta cacccgagca agcttcggaa
    68941 tctcattcta tgattcggca acttttagcc gcaaaatatg ggcaggagag cgcggctggg
    69001 acttcgattc tgtacggcgg tagcgttaaa tcttcgaatg ccgcgagttt gattcaagcg
    69061 caagatattg atggatttct ggttggtggc gccagtctaa ttgaagacga attcgcccgg
    69121 attgccgaaa tcgttgacga atacgtaaaa cataaggagt catagtgtat accttcttaa
    69181 taataattca tgtaattgtg tcagccttat taatgctggt aattttaatg caatccagca
    69241 agggaggcgg attgtccggt acatttggtg gcgccggagg cagttcttta ttcgggggac
    69301 atggcgcggc tacttttctt tcgaagttaa cgaccggctt ggcgattgcc tttatggtta
    69361 tttctctttt aattggatta atttcggctc cgcgcggcga acccaccagt attatccgtc
    69421 aagaggctaa tcgtcgtacc tcaccttcag ctgaattgcc gattccgcaa ggtagtagcg
    69481 aacagggaac tcccgtcctg cctggtaatt aaattaattg caaatttatc cagatagctt
    69541 tttgaaatag ttaataattg ccgaggtggt ggaactggta gacacgcatg tttgaggggc
    69601 atgtgggcgg atttgcttgt acgggttcaa gtcccgtcct cggcacgaaa atgaaaacca
    69661 ataaaatggg gtaaatgatg aaatatgtaa aacaaatcag tgtaatcatt ttaattactt
    69721 tggttgtgtt gagtagcctg attggacaag atcctctctc agaagcttat actctctttc
    69781 agaacggaga atatcagaaa gcatttgaaa aatgtaaacc tattgttgag aaagatccca
    69841 attatgctgg ggctgttttc cttttggggc ggatttattt tgcacttgga gaactggata
    69901 gctccaaggt ttaccttgac aaagcgattg atttagatcg tgccaatacc gagtttcgag
    69961 agattagaac ccagattgca gcctttacta ctcagctaac agaagcttct cgactagtaa
    70021 ataatggtga ttacgaaggc gctaaaaatt tatacctcga aattattaag gctaatccca
    70081 attttgcgga tgcttatttc aatctggctc aagtatatat tcgtctaaat gatttgacga
    70141 atgcctcact gtacttgaga aaagttatcg aaatgaaacc gaacgaagaa cggtatacaa
    70201 aactattaaa gaacttggtt cagcagtact tacaggaagg tgaaaacctg cgccagagac
    70261 gtaataatga aggagcgctg gagaaataca aacaagcact cgaactcgat ccgaacgagt
    70321 ttttggctta ttatttcagt gggctggctt attatgacat gaagaattat gatgaagcca
    70381 tcaaagctat caataaaggc ttggaaatca atcccgaaca tgccaagtcc tacttattgc
    70441 ttggtcaaat ctacgagaag accaacaaaa aggctgaagc cctaaaagct tacgaacaag
    70501 cagttaaaat tgatccactt tttgaagccg gttgggatcg aatcggggta ttgaattaca
    70561 atatgaaaaa ttatgagcag tccattagtg cctataaaaa attgattgaa atcaatcccg
    70621 agaatccacg ctactatgaa aaactggggc agatttatct ggataccaat aatcccaagg
    70681 aagcgattat caatttggaa aaggttactc agaaagaccc caaaaattta aaagcctggt
    70741 tgagaattgc tcaagcttat aataatcagg gggaatgtgc tcaggctaaa acggcggctg
    70801 aaaccgcttt gaaaattaaa gccggtgatg cactagctct aatcgaactg ggaattgcgg
    70861 aacgttgtct cggaaatacg gtcgcggctc gtcaggcttt tcaattagcc gccaaagatc
    70921 cccaatatcg gcgctacgta gaagaacatc tgaaaacggt tcaataaaat tttctagtca
    70981 gaataattaa gggtgagtca gtctcaccct tttttattcg gataactctt ttatgaaaaa
    71041 ttctatggct taaagaggtt tttgagtaat tttaaaagcg aaatatgaaa acaatcatta
    71101 gtaaaagaac cgactactat gcctgaattc atcctttcaa atgctgaagc cgcggcggtt
    71161 gatcgctatg caattgagga gttgcaaatt cccggcactg aattaatgtt caaagccggc
    71221 aattttgttg ccctgaaagc caagaaattg attcaggaca tacccaacag tcgcattgat
    71281 cttttctgcg gaattggaaa caacggcgga gacgggtttg tcgcggcttt gttattaagt
    71341 gaatggggcg ctaattgcat tatttggctg attggtgatc cgcaaaatat caaaggcgat
    71401 gcacgtcatt tttatcagaa atgggtggag agcaagcaga ctatccgttg tataaaaaca
    71461 gcggaagact ttgaagaata ttccgacttg ccggaatccg atttgataat tgatgcgatg
    71521 ctcggaaccg gtttccatgg tgaagttcag gggattatag ccgatgtgat acgtcgtatt
    71581 aataatatca atttaccggt aattgcagtg gatattccct ctggggtaaa tggagatacg
    71641 ggagaagtca gaggagatgc cgtcaaagcc gattataccg tgacgatggg ttttttaaaa
    71701 cgtggtttgc ttttttatcc gggcaaaaga ttcgccggtg aaattgtcgt agtagatctc
    71761 ggttatccgc gtaaagcttt tgacatttta aaagaaaaaa ctttcctaat tggtaagcag
    71821 gatgtcaaga aggttattcc cccgattgcc gaagatactt acaaacatcg tcagggaaaa
    71881 gttttgataa ttgctggttc gcccggaatg accggagcgg cgactctttg cgcattaggc
    71941 gctatgcgta gtggcgccgg actggtggtg aatgccattc ccaagagtct gaatccgata
    72001 atggaagtca aactgaccga ggcgctaacc gcaccacttc cggaatcgtc tcagcagact
    72061 ttttgtctgg aatctttgac ggcactccaa tccagactgg aatggagtga tgtggttgtc
    72121 tttggtccag gagtttccga taatgtcgaa gttcaggaat tcgggcaaca gttgataaca
    72181 aaatgtcata aaccattggt aattgatgct gatggtctgc gtattttcca taataattta
    72241 gaattaattg gtacgattga ggatttaact ataactcctc atcgtggcga atttgcaatg
    72301 atgacgggga ttgcagtcgc agaagtgaat aaaaatgtga ttgaagttgc tcgcgatttc
    72361 gtacgaaagt atccttgtac tttggtgtta aagggcgcac cgtctgtggt tgtttcttct
    72421 acgggagtgg tggcggtcaa ttcgaccggc aatccggctt tggcgactgg cggcacgggg
    72481 gatgttctaa ccggcataat tgcggctttt cgggctcagg gcttaaattc tttcgatgcg
    72541 gctatggcag gaacttccat tcatggatta gccggtgatt ggggacggaa gaagctcggg
    72601 gtcagggcat ttattgccag tgatttgctg gattatttac cgctgatatt gcgtcggctg
    72661 gatagggtga tttaatgaat cgcttctggc tgaaagtccc ggcaatttta tatgccgccc
    72721 ttatttttag tttatcatct ttaacaaaaa ctccgctggt tgagattcca atctggaacg
    72781 ttgataagct ggttcatatg ctggaatata caatatttgg gattttacta gtgttggctt
    72841 ttagaagcga tcagattggt aaaccgatgc ggcgcgctaa ctttcagagc attattctgg
    72901 gtattttata tggcttaacc gatgaaattc accagtactt tgtccccggt cgcaccagtt
    72961 cactggctga tcttggagcc gatgcgctgg gaattttact aggcgtatgg attttcaacc
    73021 gcttgaatgt ttttcagaaa tttagagaag catgagtctg actaaattaa aaattgataa
    73081 ttttgcgatc attgatcata ttgaagttgc tttcgggccg ggattgacga ttctaacagg
    73141 ggaaaccggt gccggtaagt caatcattat tgatgccttg aatctagcgc tgggggaaaa
    73201 agccagtccg aatgttatac gagccggtgc agacgtcgca acagtagaat gcacctttga
    73261 tctcaccgat ttgaatccag ccactaaaaa tattctgaaa tctcataatt tttaccagga
    73321 aaataatccg ttgaccttga aacgcgaggt aaatattaac ggtcgcagtc gtgcctggct
    73381 taatggcgaa acctgcctgg tgagtgttct taaagaggtt ggcaatttgc tggttgatat
    73441 gcacggtcag catgatcacc agtcgctttt gaatccggat acccatattg aatttttgga
    73501 tgcatttggg gattacgccg aatggcgcga acaggttgcg aggcaatttg acgatctcaa
    73561 actacttttt gagcgccaaa atttactcca ggaacaatta cgactcaatc gcgagaagcg
    73621 cgaactctgg gaatttcaat tgcaggaaat ccaaaaggtt gctcctcagg aaaatgaata
    73681 tgagaccctc ctccaggaaa aaactatact cgaaaatacc gctaaaatcc agcaacttag
    73741 ttccgatctg acttatcaat tactggagtc aggtgactca ctttataacc aattacagag
    73801 cgttgtgaag caactttcca atttgcaaaa aattaccggc tcatttgcag agcaactttc
    73861 ccgattggag gagagtcaat atctattcca ggaaatcgcc cgacaaatcg cccattttgg
    73921 cgatgatctg caatttaatc ccgcgcggtt agagatcatc aatcagcgtt tgtatagttt
    73981 tcaacaattg atgaaaaagt atggacgctc actgagcgat gttctcgcct atcggcaaaa
    74041 gctagagacc gatctgaccg aaagcgatga tctcgaggtt caaattgaca aaaacaggga
    74101 acttattaaa gaacatctgg aaaaattcac tcgatttgcc tttgaattgt ccgaaaaacg
    74161 taaacaaagt gccgaattat ttaaaatcga aatagaaaaa attctggaac gtctcggaat
    74221 cagcggagcc gacttcgccg tacgcctgga attgatgcca gaccaaaacg gctggattat
    74281 aattgatggt cagaaataca aagcggaatc gacgggcatt gataaagtaa cctttgaaat
    74341 aagtactaat cgcggtgaac cgcgccgacc gctggcagaa attgtttcag gcggcgaagt
    74401 ttctcgcatt atgctggcta tcaagacgat tttggctgga cgcgatcata ttccggtgtt
    74461 gattttcgat gaaatagata ccggtatctc ggggaaaatt gcgcgaaaag tcggtgagga
    74521 gttacatgaa cttgctaagg ttcatcaggt catttgtata actcatttgc ctcagattgc
    74581 cggtctggct gacgaacatt ttagcgtgga gaaagttact acgaacagcc gcagtcagac
    74641 tcaaattcgt aaattacttc ccgaggaaag agtagccgaa atcgctaaat tgattggagg
    74701 tcgttcagtt agcgaaatta ctttaaaaca ggcgcgcgaa ttaatggagt gaagatggat
    74761 ttttcagaat taatattaaa gaggcgcagt gttcgtaaat atttgccgaa accggtagag
    74821 cgtgaactga ttgacaaatg tttggatgcc gcgcgtttag caccttcggc ttgcaacgct
    74881 caaccctggt actttattgt aattgataat gaggaagtga aagaccggtt gtgtaacgcc
    74941 gcttttagcg ggatttattc ttcgaataaa tttgtgaaaa atgcaccggt gataatcgta
    75001 gttattaccg atcaaaaatc ttatcaggtt aaagttggtg gattttttcg ccatctcaag
    75061 tatgctttgg tggatattgg tattgctggt gaacatctcg ccttgcaagc ggctgaactg
    75121 gggctcggaa cttgctggct gggttggttc agtgaaaatg gtgtacgtaa agctcttgat
    75181 cttcccaaat ctactaaaat tgatataatg tttagtctcg gtta
//
LOCUS       DAOSMP010000004        75064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_359_length_75064_cov_14.253263, whole genome shotgun sequence.
ACCESSION   DAOSMP010000004 DAOSMP010000000
VERSION     DAOSMP010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 75064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 75064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75064
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_359_length_75064_cov_14.253263"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PKX09_01080"
     CDS             complement(<1..476)
                     /locus_tag="PKX09_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU16455.1"
                     /translation="MASIELKQIVKEFDKGAPIIKNLDLTINDGEFLVIVGPSGCGKS
                     TTLRLIAGLEEVTRGEVYIDNRLVNNILPKDRDIAMVFQNYALYPHMTVYENMAFGLK
                     LRKYPKSEIRQRVKEAADILELGDLLNRKPKQLSGGQRQRVALGRAIVRKPKVFLF"
     gene            complement(584..1114)
                     /locus_tag="PKX09_01085"
     CDS             complement(584..1114)
                     /locus_tag="PKX09_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOU16456.1"
                     /translation="MSNILILYDSKTGNTAKMAELVALGARKNGKHNVRILHIDNAGA
                     EDIRWCDGIALGSPTNMGLISWKMKKFWDDLGDELWGKIEGKIGCAFSSSGGWGGGAE
                     ITCQSALMILINFGFLVFGVTDYVDEQLTLHYGATVAGEPRSAAEKAACLRLGERLAE
                     YLSKFKLQSDKNINEV"
     gene            complement(1178..2497)
                     /locus_tag="PKX09_01090"
     CDS             complement(1178..2497)
                     /locus_tag="PKX09_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU16457.1"
                     /translation="MKNRKIKRLSIVGFVFLIVMANNCSKPKPKDANSITLTFWHSFV
                     ATTLPILEAMIADFEEENPGVKIQAQYVPTGDALVQKLITAIQSQTAPDIAWIHSDFL
                     DNLVNSRAIFSINELAGDDSLFYNQCLPDIFPQLLPPITFRDTLFALPMEATTLALLY
                     NRDWFRQIGLNPDHPPATWAELDSFTMQLCSDSNSDGQPERYGFYVPVFPASGPLNIW
                     MTMQWAPFVWQAGGEIFSNNQPTFASEAGVAALAFWSKLYHQQKFERFSMAHDMGFIS
                     QTVAMIMDGPWNLPRYRQYVKFDWGVAPLPAGSAGRASYLAGESLVVFKQCINPEEAW
                     RFINWFLKPEVQRTFAEKSGYLPVRRSVLNDSDYVNFLEQFPQQKSFNEQIRYARGRS
                     LPDKFRVEVNQCIAEALESSIIGRIAPEKSLKVADEKIRRLLSSETE"
     gene            complement(2487..3080)
                     /locus_tag="PKX09_01095"
     CDS             complement(2487..3080)
                     /locus_tag="PKX09_01095"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU16458.1"
                     /translation="MPVISYKNEPFPDWSEVKSCKIIGLSKRQKLQLPSEFSETLLFV
                     LDGVAEISEGAEKRTIFSGDVLDQFFGEVWIEPKPLVELFQISGNWSNDRGSCGVFRL
                     GKSEHPRNDGDPVEYERNTVFDNHFHDCDEYWFIVKGKGIAVSEGKSYNVQAGDCVIT
                     PAGEHHDFPIVQEPILAVWFEGSMKGKKRPGHLYREK"
     gene            complement(3061..4284)
                     /locus_tag="PKX09_01100"
     CDS             complement(3061..4284)
                     /locus_tag="PKX09_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16459.1"
                     /translation="MNEIISKLEKLIEHNDGILRLKPAWVARDFLPPGRRLGLPEEMY
                     DLGERGGICERWVGSTTPADNRIKVPNEGLCCLDLEDGQEITLKAAVEIAPALIMGAD
                     YAKAHTGLGRLPKIFDYAYRLPYHLHQMAKDARKVGRNSKEEAYYFPEEVPLGPEPET
                     YFGVHPYISLEKRYELLLPSMQEWKDDSILLHSRAYRLKAGDGFHVPAGILHAPGSAL
                     TIELQEDSDVFAMMQAKVGGDIIIDKELLFKDVSEKERKNLAEKAILGMIDWELNGDP
                     YFYENRHTPPILIDSDQQSGSEQSWIFYNTNKFSGKKLIIRPGGKHVSVEKGVYNILA
                     WRGQGTYGNVEIEGLNFEKDELLICHQRAIYPIEVVNTGDRDLIIYKFFGPDINPDVP
                     MLTKYPKSPNACNIL"
     gene            complement(4306..6936)
                     /locus_tag="PKX09_01105"
     CDS             complement(4306..6936)
                     /locus_tag="PKX09_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOU16460.1"
                     /translation="MINSCDKTQDIDLNGRWQLAPDSTGQITINSIDQIKNWRMTEIP
                     GSWHRLHVDLLDYQGVVWYRKLFKLKSLPPKHRFLLNFGAVDYLAKVYVNRELVGEHE
                     GGYTPFSFDVTEQLQKGENEILVRVVDPVADKVGTDGISYWHIPHGKQNWYVQTSGIW
                     QAVQMAIKPEIYVSRIQVNSQISGAFQAKVFIENARAGATPVELILTIRDPNGKVVFS
                     DKYEGNSSTSEAEFTGQINNPRLWTIGQPNLYTLEIRLGEDVLFDRFGFREIVVRDGK
                     FYLNGKPFYMIAALDQDFYPETVYETPSEEYLHDEMHKARELGLNTLRCHIKVPDPRY
                     LKVADELGLLVWYEIPNWDVFDESVKARSRETLREMLQRDWNHPSLAIISLINESWGL
                     DLNQANQRQWLKAEFDFAKSLANGRLIVDNSACWGNYHLKTDINDYHTYWAIPENHQK
                     FSETVQEVADRPRWLFSPYGDAEETGQEVLMISEFGNWGLPRLPEKLPFWFDRQFLDN
                     LVTLPEGVIQRFNDYQYGRMFKNYNELAEFSQKAQAQVLKWEIEEIRLQPELQGYVIT
                     EFTDINWECNGLLDMWRNFKADRQILAQVQQPDIIIPRLTKYGFWDNEEIDVQLSISH
                     YSNRDFSNVRIQGIYNGEIIGDKSIAIDSVGVYFVGSMKINSEPVKQPVSQRVKFRLL
                     SREGDVLAENFIEFTVYPSRKIENISPNSNLLICNSIDLKTMDHLKNGLDVICVIDSS
                     SVLPANFPFTITSRESDGYDGNWASNLNWVDDSHRPFNNLKSNKVLGFEAYKALPKYA
                     ITGIQPEQFVDVLAGMYVGWLHLNSAYLMQMRVGSGKLILTTFNLIQNYGDDPFTTTL
                     RDELVAYLQSEDCQPTINWK"
     gene            complement(7090..9441)
                     /locus_tag="PKX09_01110"
     CDS             complement(7090..9441)
                     /locus_tag="PKX09_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU16461.1"
                     /translation="MRNIVKIITLIGLSLASTLAVELQWQTHNIEVWQPEQVIWGKID
                     TLTATSGILYWKDGSKIFDINPSDSSFYIEVKLDEGENWFLATIENQGEPIYSDTLRL
                     QMKMSLKPILEAYATVADREVTLHGKIIENPDSNAVTFTWSEDERNPAIFGWNGTSDS
                     VLVFNVPEQAPAGEYYFDLIGVTTSGDTTRARTFITVSFDGIKPFDIYRDHAAWIDSA
                     VVYEISPYAFVMEGTFQDIQQKLPELRELGINTIWLQPIFKTQWDEQGYDVIDYFTIR
                     SDYGSESDLQNLIKSAHTLGMKVLLDIVINHTSIYHPYAQHSLKYGTASHYYNYYQRE
                     IDNAPYSQYYNYYKGFINYFWDELPNLNYDNPEVRNWMMTACKYWVEKFDIDGYRFDA
                     VWGVNARCPEFTQNLRLELKRIKPELMLLAEDKASWSMVFDQRFDVAFDWAPGEGWVS
                     QWYWEVDYSEDATKQFTIFNYSNPSERVNLLRQALTNNGQGYAANAKILRYMGNNDMI
                     PFIKNHNLAQTKMVSTLIFALNGVPMLYNGQEIGYDKHPYSSYQIFQRGKSIRSQDKY
                     GLFDHYRHLIQIRQKYSALRSDYFKEIPVVPAYYAFAFHRRSGDENLFSVINMMSEPK
                     NITISLPIEELHLDSTKTYYLSDLITGMTFSGKPENLSSVALTIPGYTTSLFLLADSM
                     VNVNIAPEEHPAIAEKFTLFQNYPNPFNSSTTIRYTIPQKSNVKLTVLDLLGREVMEL
                     VDGEQNPGEHQVLIRTDQLASGCYFYRLKYKDQILVGKMLLLK"
     gene            complement(9472..10620)
                     /locus_tag="PKX09_01115"
     CDS             complement(9472..10620)
                     /locus_tag="PKX09_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOU16462.1"
                     /translation="MTSKERLLIALKGGVADHVPVSPDISVMVPDRLDGRPFYEIHLD
                     GREHYGWTSATHGEVYVKAVKYFDMDGFYMYGGLKEVKVSSSLNFESETIDIPAGKLV
                     KRTWHTAKGDVTEEEIFFADESPWRSVKPIKNLQEDFEKFQLVMGKGDWQWEKEFRDK
                     QVIGDLGVYLGMIPVFQDWWFSNRDGGFENVFSDLLMEADYMQKVHEYWMTWALAYLR
                     AMIAAKPDVIMLGGSSASLSVSSPDIFRKYELPFIQQASSICKAANMPCHLHICGRSW
                     PLVQIVAEETDVTSMEPMEEPPTGNVDIAMVKKRYGRKLNIKGNINTTEFMMRATPQQ
                     VEDKCKRLIDDVGYDGGFVLSTGDQCGRDTPDANIFKMVEVAKTYGKY"
     gene            complement(10631..12307)
                     /locus_tag="PKX09_01120"
     CDS             complement(10631..12307)
                     /locus_tag="PKX09_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOU16463.1"
                     /translation="MNNDPSLKKTLSRREFIRKSTMGIVGAAMFPSFLEKSNILAKTT
                     KPLLFKPFPHPWMPPLNFVYAADTNGDPFQSGIVVNGDGIVVPEDLGTRRFSVNCQWF
                     IEGFGNCFLSADNDGEFYSLKEMTGRNELILNHEFARSRIARNRQVMRRYQTQGTKFS
                     PEVIRLSGLAEEYLEDSAKYTAESEKCASIADKALYYALWTGENIELEHARQMISKNN
                     RHDEVYFGCESRQYVWAKNEEFTKRFVELFNFATVTHYIWDTWYELFEPREGVYNWGI
                     KDNIVRWLTEKNITIEGRPLFWFHPTVTPEWLKSKNYDQLRSYVDKHISDVVGHYGDQ
                     VLHWEVINEYHDWANIFNHTPDQITEIVRQVCDKTHEINPKVDRLINNCAPWGEYVAR
                     GRMARMEATRPLRTPYQFITDLLDAGVEFDTLGIQIYFGNRDLSDIMRMLERFEKFGK
                     PIYITEIGTTSGPTFETVVDRSLGISGKPYDWHRAWDEELQADWLEQVYTLYYSRLNI
                     KAINWYDFSNFRPFIVNGGLVTESCEPKQSFYRLKELLRSWNRLPDNIKK"
     gene            complement(12308..13855)
                     /locus_tag="PKX09_01125"
     CDS             complement(12308..13855)
                     /locus_tag="PKX09_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOU16464.1"
                     /translation="MPNKTLIFKPYFIQKGRGPHLDEFAYLMDMNGDTFHSDVAVKNQ
                     GVTISNTKGIKKFTLNVRWNVEGYGYLFMPADNGGEFYSLPQFGSLHLNLNFELAKTR
                     IYRNRRRQANFNREGWLASRELSAMINLAENYLDDAIKNVQDDETCARFSQQALLYAL
                     WASEMLELEKARWNIAKNSARLDFLFGCDGRGYFQMDSELFLERFTEVFNYATITHYL
                     IGDFVDFEPVEGQKQFAERDRLLADLEKYKIQVAGRPLFWTHKWVTPDWLKNKTFPQL
                     LNYLEKHIRTVVGHYGNRIKVWEVVNELHDWANELFLNHEQTIELTKFACDVVRDVNP
                     NLKILINNCSPFGEYVQKGLWHELPARYPQRTPFQFTEQIIEAGTDFDAIGVQVYFTS
                     KTLADYIGVIERYEKLGKAIHLAEVGAPSRGITQEFLDEEPGDYSTLPYEWHRHWDEE
                     LQADWLEAIFTYAYSQPMIQAANWYDLIDGFSFLKNGGLLRSPKGERKAAVDRLKKLQ
                     EKWRILQ"
     gene            complement(13876..15549)
                     /locus_tag="PKX09_01130"
     CDS             complement(13876..15549)
                     /locus_tag="PKX09_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOU16465.1"
                     /translation="MPRITRRKFLKMATYAGLGGIVGGRALSAPLVLTPKRTKTNTEA
                     GELIFKPIIVQKGRGPHLLDWAYASDEHWDAFHSNITASNEGVKISDTEGTQKFGVDV
                     RWNVEGFGYIYITADNGGEFYELPPKGKQRELNLNFELAKSRVLRNRRRLELLRRDGW
                     QPSREVKELLALSDSLYEDAVKSQASPEICGDYAQRALGYAMLVGEKMELEKADFEIR
                     KIGYRPHFFIGCDSRAFYQMDPELFMERFTEVFNYATITHYLLSGYYQDFEPIEGQKN
                     FNLRDAVFKELRQNGITVEGRPLFWFYNSTTPDWLRHKSYDQLLKYVESHTKEVVSHY
                     GEGMYAWEVVNEAHDWANELRLKPEEIVNVTKLACEVAKATNPKVHRLINNCCPFAEY
                     VQLKKWGELDAIYPQRTPHQFMQDLVDAGVDFTITGQQMYFPYRDLQDTIILIERLEK
                     FGRPVQLTEIGASSGPTKDSINTGQLGMPTEPYVWHRHWDEELQADWLEGVYTLAYSK
                     PWIEAANWYDFVDPYAWIKTGGLLRSPNGERKAAWQRLLKLKEQWKNLQ"
     gene            complement(15561..17156)
                     /locus_tag="PKX09_01135"
     CDS             complement(15561..17156)
                     /locus_tag="PKX09_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOU16466.1"
                     /translation="MANIINSQSQAPSPSGELLFKPFFVQKGVGPHLGDLAFATDENG
                     DTFPSNMTIDADGIKISNTKGHKRFGINVRWNVEGFGYNDYTADNCGEFYELPIIGKQ
                     TVYNLNYEIAASRVARNRQRRVNFVKEGWQPSKETSAYLDLSEGLLEDAGRKSRLSED
                     CAQLAQKALHYALWGSEMMELEKAKWNIKRIGRRANFLFGCDARSYYEMPKDRFVEYF
                     TRLFNFAAVTYVVNGDPEMDIFEPVEGQLNFTHRDILCNVLRQNGIKVEGRLLFWFHK
                     WVTPEWLAHKNFDQLKKYVEAHTREVLGHYGDRMAVWEVVNEFHDWANEVQLNPDQVV
                     ELTKFACEVARDTVPNIPLMINNCCPFAEYVHLGNWSGQKALYPQRTPWQFVRDLIAA
                     DVDFDIIGLQYYYQYRDLQDYIVNMERYESFKKRVQISEIGATSGPSEESVKNGSLGF
                     PTEPYSWRRPWDEELQADWAEALYLIGYSKSWIDSVLWFDFIDNGAYYDNGGLLSGRG
                     RHPKVAYERLIKLRQWLETSGTK"
     gene            complement(17206..18240)
                     /locus_tag="PKX09_01140"
     CDS             complement(17206..18240)
                     /locus_tag="PKX09_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOU16467.1"
                     /translation="MNLGHYIKVIILGTMLISVIVRLEYCQEESEIPVTFTNPVVTDG
                     ADPWVIRYQNFYYYCFSTGDRIWISCSRNLHEIGLAQPTLAWQPPKGNPWSEELWAPE
                     LHFFDNKWYIYVAADDGANENHRMYVLESDNIDPQGHYVLVGKIADSSDCWAIDGTIL
                     EYNDRLYFIWSGWEGKVNVQQNLYIAPMSNPWTISGDRVLISEPEYEWEKVGRPLVNE
                     GPEILKKNDKIHIIYSASGSWTDDYCLGQLTFTGGDILSRASWIKKPEPVFSKTETVF
                     GPGHASFVKSSDGREDWIVYHAAKYKGSGWDRDVRMQKFGWNNDDSPNFGAPVSAGVL
                     LEVPSGSFKK"
     gene            complement(18247..20454)
                     /locus_tag="PKX09_01145"
     CDS             complement(18247..20454)
                     /locus_tag="PKX09_01145"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOU16468.1"
                     /translation="MRFKISLFLLFFLFINGCSSSGINDLNQFVNPFIGTSGGGNTFP
                     GALVPWGMVSVSPHTDLKAPSGYIHGEPYFYGFGQVHLSGTGCADLGSVIITVTRGEV
                     KTEPEQYRCSYQNEQAKPGYYAVNLNEPDVKVEVTATTRCSFSQFTIGKEGNVNILID
                     AGRSLVERQGGSVRFVSPTEIEGYNRSGGLCGEDNQHQVYFVTRVSATPQKFGTWIDD
                     QVSEDISRASDESSIGAWMTFTCQNGQIVNIKTGISYVSIANARQNLDAEITDWNFDK
                     IGRNAEREWRKQLSKIKVEGGSEAGLTKFYTALYHCLIHPNIISDVNGEFPLMGQTGV
                     GKYTDRERYSVFSLWDTYRTLHPLLTLIYPEQQSAIIRTMIDMYSESGFLPKWELAGN
                     ETYMMVGDPAPIVIADSYLKGIQDFDPDLALEAMLKPTLLDSGESAPPIRAGYHELLK
                     YGYIPFEQDTSQAWWVWGPVSTTLEYCLSDFCIGNMANMLGQNQIAEEFGKRASFYRN
                     LFDPETRFMRPKLRNGQWLEPFDPLATEGSGYWEGSGGPGYVEGNAWNYTWFVPHDVS
                     GLIDLFGGYEPFKIKLEQCFSEGHFTITNEPDIAYPYLFSYIPGNEYRTAELVQEILA
                     SDFGTESDGLPGNDDAGAIAAWLVFSALGFYPACPAVDEYRLGIPLFQKVTIELDKEY
                     YSGKQIVIEKSGKLVNSARLKEILFDGERWDSFGITHKRLTEGAHLKFVYNQE"
     gene            complement(20472..21614)
                     /locus_tag="PKX09_01150"
     CDS             complement(20472..21614)
                     /locus_tag="PKX09_01150"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HOU16469.1"
                     /translation="MEHSKRIYSLDVFRGATIAAMILVNNAGSYDYVYPQLLHAEWHG
                     WTFTDWIFPFFLFMVGMSMVFSFESRLKHGDTHQQILRHSLKRLLILFALGLFINGFP
                     FGLIPGSQFSIATWRILGVLQRIGICYFFTSLIYLNVKPKGQWLWIGILLGIYWFALK
                     VIPVPGYGAGVLEPEGNLAWFIDKTLLGKHTWAFAPVFGFDPEGILSTIPAIASTLLG
                     VQAGRWLRSERSQEEKTAGMFVAGNLCLLLGIIMNIWLPINKNLWTSSYVVFINGWAL
                     NCFATIYWIVDVKGKTWGTKPFVIFGMNAITVYTLSELLANLLWAISWNRADGTFTTL
                     HDAIYENVFLPIASPVNASLLFAIAFVLMMYAVAWGMWKKRLFIKI"
     gene            complement(21623..23944)
                     /locus_tag="PKX09_01155"
     CDS             complement(21623..23944)
                     /locus_tag="PKX09_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16470.1"
                     /translation="MKNWLYLWLIIILFGNELNAQMLNPSIDRDDEPFCYFSKPTDQI
                     GVMDGREGTLITPEGYLYTGSGEMMFFTGNPPIPTEKRVKTLLNGYLPVIQYTLNRDG
                     FDYRWTIFAATLDGKPESPLVNFVRVKIINRNSQQRTTWFAAGIRYQNEANTDWGIGD
                     NRFIRPAVPEQIGEYEQAGLEFRQDWLCLHQDDAVLYNDKLLYSFPPREKIRKFLTWK
                     MGYNEPPAEGEMTLYVLPTTPVGIVQYPLTMLPNEEVVLDFKFPYMPVDTVDEYSKWI
                     RQAQFDHYLNKTIQFWENILQEGMTIVLPEKKVTDTFNANLINSLIARDKIDGWYVQK
                     VNEFQYDNFWLRDAAYFTRMYDLTGYSDEAERVLDFFPRWQREDGNFVSQGGQYDGLG
                     QTLWAYGQHVRMTDDRAFAEKVFPSVLKAIAWLESARKTDPLNLIPVTTPGDNENITG
                     HVTGHNFWTLIGIKNAVFIADYLGEGEKSQQLQKLYDDYYATLMRILAEITAQTGGYI
                     PPGLDGEGGEDWGNLLSVYPEVILSPNHPWVTQTLQTVRSKYQEGIMTYGGGRWLHHY
                     LTLRNTETALVRDEQETVIKELYAILLHTSATQAGFEFSILPWKDRDFGMNLAPHGWF
                     AAEYRILLRNMMVREQSDTLHLLSAISPNWVNKDAKIGINNAPTEFGEVNFTLTFKKN
                     SATLVLKNNFKKAPAVIILHLPWFMEVRKIEIDGRSINQENNAVSLPVDTRQVDLEWS
                     FKKNIENMSYDQTVEQYKSEYRRRYEKFIREGD"
     gene            complement(23953..25323)
                     /locus_tag="PKX09_01160"
     CDS             complement(23953..25323)
                     /locus_tag="PKX09_01160"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOU16471.1"
                     /translation="MTGQMIKKSLIVYFVVMTIRGIGILQAQAITPDPRSKVDILLVV
                     AHPDDETAIGGQLAKWIFDDGKKIGVIYTNRGQGGGNSIGKEQYHSMGAIREIEARRA
                     LSAFGIYNVWFLDGIDTPGQDVLHSLQNLPHGAALEKLVRYVRLTRPEIIVTWLPHFV
                     AGENHGDHQAAGVIATEAFDMAGDPTIFPAQVAFPRETMDINNFYEGLRPWQVKKIYY
                     FSDRDEALNAPGPQFDISAKSPSQGKPYIELAAQLHKFHQTQADVAQIAEQAEQSGNW
                     QPMIEWLEKFHLIYGKSVVKCEPTGDVFEGVSEKPADFHKNTGYISPVARGVKLQFGG
                     IFDYYRHFWQVHDLQHLATIVSPEIAISVGSYFHVPLLITNGDKKTIRLSVKPILPNG
                     WQEVSGSGEYIIPAGESLPVQCFYFAPDNETDGFQPLKWQIIQDSKVLDEIVMNVKLV
                     EWALPQ"
     gene            complement(25340..26626)
                     /locus_tag="PKX09_01165"
     CDS             complement(25340..26626)
                     /locus_tag="PKX09_01165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU16472.1"
                     /translation="MAKYFKEPLLINPEVRRNFLLNWMDGTFFAFGMSFVPVMTVLPV
                     FVRQVGGNNITVALVQVIWIIGVNFPQIFIANYTRQLPYKKPFFLKTAALQRLPWLLM
                     ALLCFFIIKNVSSNMGLVFFFIAFGLAAIAGAVNYPGWFDLISKITPVNLRGRLFALR
                     TITGAILGIAGGWLVKIILSAQPFPANFGYLFLLAFVMMTVSYVSLIFLKEQEANAST
                     ESLNHFQYFRQLPNLIKSNHNFLNFLIADSLMTIGLIAQPFFIVNAMQRFGLDSASSG
                     SFTMIYMLSMVILNLCSGSIGDRFGHRVNMIIAAFSVLASCVLAITLRSLSLYYFVFV
                     LSALNTSMIQVSRLPILAEICNEKERPTYISISNMITSPFILLGLLGGYLVDLYGYDV
                     IFAIAGLGGFLSLIWWVFYVAEPRRQNIFVVESVER"
     gene            complement(26610..28757)
                     /locus_tag="PKX09_01170"
     CDS             complement(26610..28757)
                     /locus_tag="PKX09_01170"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOU16473.1"
                     /translation="MRNILKESYIPFGSQYYRAPSPSRNEWENDLKNMADNGFNTVKF
                     WVQWRWNQPSEDRFCFDDIDELMDLSARFGLKVMLNTIFDVAPAWIYHKFPDASMVTL
                     SGRKIGPQTQPHRQIGGLGYCFNHDGVMQHFFQFLAETVKRYTNHPALAIWNVGSEPE
                     LTSSMAEMREYADDASKMGDMLCYCDNCRVKFRNWLELKYQTIERLNISWNRNYTSFE
                     EIELPLTRNTFNDIIDWRMFFVATLGENVRKRFEVAKAIDQGRHPLMCHHVFIQGFPV
                     TSTASDPWNVGRFGDLHGFTQMDDPAMIDILRSCAKGKPVISAEMLMLNGYTLDLPRS
                     IDINDVKRFIFSGIAGNLKGFIFWQYRPEILGREAPTWGLTTLDGEKTSWLQDFARVG
                     QVLQKNADFLLEAQPQSAEIAISYNPENQIFIWAATGNEKNATNSLLGAHKALYEHNY
                     NIDFVHPIEIDEKLLSRYKVLIFPFPYLIDQRICHLLEDWVKKGGLLIGESYFAAWNR
                     ENGHHEKIVPGYGLSKIFKARQGVVDPVNTNDLVDILVDSDVLDNEEEIVVKGAIVQE
                     RLISEGAKVIRYFPDGSPAVTLAELGLGKAVLIGSYIALPFYNKGIQANGDFLAGLIK
                     KYANISTNYSDSLGQIRVDLLKNESGKMMLILRNLTETAINTEIHICGLIPKHLAEQF
                     SAETLNVVYQDNDSSFNITLNPKEVKVYFGEIF"
     gene            complement(28792..29718)
                     /locus_tag="PKX09_01175"
     CDS             complement(28792..29718)
                     /locus_tag="PKX09_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004404801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOU16474.1"
                     /translation="MKLGFLTACLPQLGLEEIVKWTAEQGFQALELAAWPVASDRDYQ
                     ARQIDAANFSKQDAERVKELFNKYQIEISAFAYYDNNLHPDLKQREYYHNHLKKVINT
                     ASLLGVELVGTFVGARPDKTPKENIQEIGCVFRELVKYAEDRNVKLMIENCPMENWLK
                     FGLPGNYAYSPELLEALFNEVPSENFGLNLDPSHLYWLGIDYIRVIKDFAPRIFHTHA
                     KDAEILPEGQYKYGIFSRQIDPIPWKSGWWRYRMPGSGKIDWGLFIRTLQEFGYDYVL
                     SIEHEDPVWEGSVEKVETGLKLGLKYLKQYVI"
     gene            complement(29750..30901)
                     /locus_tag="PKX09_01180"
     CDS             complement(29750..30901)
                     /locus_tag="PKX09_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020155584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU16475.1"
                     /translation="MKTIKVGIIGAGFMGTAHLENLRRIPGIEVKAIADIKPENAKIL
                     ADKFGISQVYENWRELVNNPEIDVVHNCTPNFLHFEINKAAILAGKAIISEKPLTNSV
                     AESEELVKLAREKKILTAVNFNYRNYPIIQHARQLIVSGKLGEIFLAHGHYLQDWLIY
                     PEDYNWRVEPEMGGNSRAIADIGSHWCDLVQFITGQRIIEVFADLVIIHKSRRKPQSD
                     VATFKGKEVTLDSTKCITVKTEDAGAVLFHLEKGGHGVFNVSQVSAGYKNGFDIAIDG
                     SQMSLAWKQEEPNQLWLGYRDKANQILVKDPALLEADSRSFAHYPGGHPEGYPDTFKN
                     LFLNFYAALRTGKLAIHEQNFPTFEAGLQENRIVEAVLKSHQEKRWIKV"
     gene            complement(30898..32517)
                     /locus_tag="PKX09_01185"
     CDS             complement(30898..32517)
                     /locus_tag="PKX09_01185"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOU16476.1"
                     /translation="MSNSCQNSRSSRIGDDSNQLFLIRTYRAVSEPALLTQKIEYKSS
                     VDGYDIVLQDNSGQFTCQVHCTFTQSGLRFQAKYSAPEPIWLVEWSLTNLNLAEVIIP
                     ALGGQVITDQMPEQTTLSYKYPFWLNAQFVIGIKGNRGLIIYSHDSSSNLKLARVRRE
                     NDSFTISYGFEPPAKKRLTSFETEWFIEEFTGDWQKPVAEYRNWMEKAFKIKPLPQKQ
                     NFPNWAKNINFILEIWGARRESQKPHHTFEQMTERLNEFKNLHDPRRTLLYLPGFAEH
                     GIDSHIPDYNPSDLLGGEEKFKELVDTAHLLGFKVMIHTNGLGMTYTHPKYPEFKKYQ
                     VVDAFGRLQGWAMDIDGDWLTEPYFAYINPGARAWSDLMIETLGRLINKFQVDAVFLD
                     QTLLAFNVKRGPDFIKGMRTHIERLQKAFPEILFAGEGLHEQVAGCLPVAQIHGIDSL
                     SGVHGIEGQEPWRKVHPVSTYLFGNYSKYVAHLLTRHPSNPTFKLQESAYSQLNVIPA
                     LCLYENDQAIQTPETYQMIDRAKRVNEGGKQ"
     gene            complement(32514..32990)
                     /locus_tag="PKX09_01190"
     CDS             complement(32514..32990)
                     /locus_tag="PKX09_01190"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF016040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HOU16477.1"
                     /translation="MNIPVKKIIPINPINFAKFGKVVALPREKPTAEGDTFKFWANIA
                     NYRINGKTEIGLCTVTLPRGNRVNSLECHLHTPEILIPVDAPFALPLLIEGEPEENLK
                     IFRVNVGEAVVINPGVWHGACIPLEKSEATYFVIFRYQTSQKDIEKKSINDIEILI"
     gene            complement(32995..34440)
                     /locus_tag="PKX09_01195"
     CDS             complement(32995..34440)
                     /locus_tag="PKX09_01195"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOU16478.1"
                     /translation="MKKYYWLQSFLALIMLIIVDCSRPNSVNYQRLLKSKDAEFVHIP
                     VGLCEDYPEETTTMEIIHNDMELLKRTGVDLLRISFGWDAIEAQKDQYDWLFWDDYVK
                     TAVNQYGITLIPYICYTPLWNSRGDTLWFWNDQPLDFEEFGEFMYDLVTRYKKYIKTW
                     ELWNEPDLDIYWDTRDVAAFAEFIKIGAQAVRRADPEAKVVLGGMAYHADFLEQLFRD
                     YGLSPYIDIVNMHNYYETWSERPVEKITENINAMAEVIEKYGDDQSLWMAEVGYSTFR
                     QGSYVSDSYYAYYDYEHTPEYQAVDLFKCLTLAVAEEKLSAIAWYEIKDLPAGEEVIG
                     DNYNNRYLGIAYADYRPKPAEKALVFFRKLFGNPYRNVTSLISVEHQPDSDVFYRAFE
                     KEDGTIIFVAWIATNVPSKRPLKPANLVQDTRTETISVTIPAKLKGSVVQYNELGSAS
                     TFNEFQRNDDQTKLNNIVLKGGEIKIFKLEK"
     gene            complement(34536..34937)
                     /gene="rbsD"
                     /locus_tag="PKX09_01200"
     CDS             complement(34536..34937)
                     /gene="rbsD"
                     /locus_tag="PKX09_01200"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HOU16479.1"
                     /translation="MKKHGILNSHLSMVIASLGHTDQLVICDAGLPLPREAEIVDLAL
                     VPNVPRFIDTLKAVLEELIVEKAILAKEIESINNGILKEVKDLLKGVPIEYVSHEEFK
                     NIYRESDSAVFVRSGETSPYANIILISGVDF"
     gene            complement(34954..35874)
                     /gene="rbsK"
                     /locus_tag="PKX09_01205"
     CDS             complement(34954..35874)
                     /gene="rbsK"
                     /locus_tag="PKX09_01205"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOU16480.1"
                     /translation="MTKNSVIIVGSANIDLVAQVDRYPQPGETVFGKKFGIYPGGKGA
                     NQAVACAKLGADTIFIGKMGDDIFRDRLVNNMESNGVHMEEIFIDSETPTGVALIYVD
                     ADGQNEIVVVSGSNMRLTERDIRSKEKLFERAKIVLCQLEIPLSAVEAAAILAKKNGA
                     MFILNPAPACKLPASLLNGIDFLIPNEIELSFLTGQSVTDRASVISSSRKLINTGVCN
                     VITTRGENGAILVNSDRWQEFPATPVKAVDTTAAGDAFNGAFAAALASGKPVNEAIPF
                     ANKVAAWSVTKQGAQTSMPTLADISNYLNQ"
     gene            complement(35885..39148)
                     /locus_tag="PKX09_01210"
     CDS             complement(35885..39148)
                     /locus_tag="PKX09_01210"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOU16481.1"
                     /translation="MKLLNELWPDKELNCENFKNPPLEMGILPFWFWNGEMDEAEMEW
                     QLKEYKAKGITGLFIHGRFGLKIPYLSEKWFERVKFAVKKGREIGLNIWIYDEMNWPS
                     GTAERQVIKKYPYLSQKYLEMVTLFINGPLFIYLEATDSRYINTGDSKPIAAYICSAE
                     EYRTGIKNIIDVTPNLSFNKVIPWEAPAGNWVLMYFLEKEIDYYIDALNPEATKRFIE
                     LTHEQYKTAVGAEFGKIVPGFYTDEPAMHYFQVGTDNYVIPWSQRMFKIFRERNGYSI
                     KPHLAALYANVGRDTARIRYDFWKTLSDQYDDSYYKQLRDWCAENGVLFTGHILFENW
                     LRGHARCEGNVMQHLSNLHLTGVDHLYPKIGSEEQPGEHVELKMASSITHHLGKKRLL
                     CESMGGTYWDCTLERMKWIANWEYSLGVNLFNNHGYHYSIEGERKRDWPPSQFYHHTW
                     WKYYNEFTRYMARLGHALTGGKHIAKILILYPITSIWANYVPQAPDDISRLIEMDFNY
                     LTDALLHLHYDFDYVDEDILINAQLNGNRLEIADESYELIILPPVTHIKKATLDKIKA
                     FVNNGGKLIADILLPVEQFIDGKPSKSRAVDELFGVKPNCILADFQSGKNEVNQLKML
                     QPGVCLIQGGGLAKDRRRQDIKEAIESCITADVQIGHSEVFYLHRVKDGRHLFYFVNT
                     LQQDIGEVDISLETVGRPELWDPATGAVKELSVFDIINNRLCFRLTFGASQTFLVILH
                     NDLPDSYISATNLVIEKTEANFITGYIKNGDLAKLTLKTGGKQKDIVQLVPKNPPPLI
                     LPEVMNFKIENDNTLCLAKWKMLVSNECSNDNYAALDYDDDHWLPVTIGAWEMQLPEE
                     PDDIHYPQTLWYRIPFQIEELPNRVKLLIDGFSGSSFRLFINGTEVKDTGQRSQLDAE
                     IREINIHSYVHPGRNLIAVQLVVQRRTDGILDLIKITGDFALKLNDDGYSIVLPKKEV
                     NIGDWCQQGYPYFSGTGTYSTELTIPADFCKGKLFLDIECGEDVLELQINDSGPIIVP
                     WHPYQIDVTDLVRPGKNTFRFSITNTLINTLEAVPKSSGLLKPPVITNYQLLKIKIY"
     gene            complement(39378..40376)
                     /locus_tag="PKX09_01215"
     CDS             complement(39378..40376)
                     /locus_tag="PKX09_01215"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOU16482.1"
                     /translation="MLHNRKIKIVTLSAMIFFATNLRVEGFNKAGRTSLQFLKIGIGA
                     RSSAMGEACIANLNSIDAVFWNPAAITQMQRLESSFSYTKWIGDLNVTAGAVGLTVDR
                     LGSIALDYIAMDYGDIPEALVTSPDGKLDTRTGLNFSGGDLSVGLSFSKKFTDRLSIG
                     IRTKYLREDLYTYSTSLWAFDVGTYYNTGWKGIRLAMTAQNFASQARWLKTQQREEQQ
                     YEVPLIFRIGTSIDLLGGKDLFLGGNPNKQRLSLNIDAIHSNDYAERLNIGAEYWLWN
                     RIALRTGYRTNREEGHFSVGAALNIPMDFTNLVVDYSFVDYDYLNSAHQFTVMLRF"
     gene            complement(40421..42628)
                     /locus_tag="PKX09_01220"
     CDS             complement(40421..42628)
                     /locus_tag="PKX09_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16483.1"
                     /translation="MKVKNYYIHLVIILGLLTGWSESFAQRKSTHTRGKLWETLNNVG
                     LIGDLGVWDYEETSGLGFYPGFSGYSFPNNEQLANGWITDANFHNFRSGPALVIKGGD
                     VLVAPDYDPQYQEYLFYHSSFAGGSHGILWNYPPFIKTNNFVGTSQFNPLLPEEINYC
                     EFPTATGISVKMRSMAWSFPDYDDFIVYDYVFINTGEMVIPALGEVKHYEQTFDSVFV
                     VFHSGIQVSTKGHLNFHYDPDFMSSAAPAGGFGGWKQLENGGTYTDYFAVENDGDDSK
                     GLFFYSRDFNGGREPHPNDTYSKRSDWQTRLKYNQSYENIELQDPACFGFVFLYRTPP
                     TDSDADPFEADPNYFNIYSDEGEQFQGRALDMNEYFSIERLGEKGMFDFITHNRIPEE
                     NNGDMYCFYTATFGPYYLAPGDSVRLILAEVAGSLDYHEVICGDPNNWYPDSSIADIR
                     RNAEAARRALKWGFGATVEGINLAADVPESPPAPNCSATNASQGSDTAIIAIQWDKLA
                     EEAVITDGAGNKFYDGSTDLSGYRIFRGIDKRGIWDEVVDIPIEEANRYWNTEIGKYV
                     YLDKDLQFGFDFRYYVQAYYSNPKTWVSANHTIVDNLGELVSDDYNMTPLTGARPGPV
                     DITAGWDVFVAPNPYVEGDPERSFGDPTPYKIEFRNLPERATIKIFTVSGDLVKTIEH
                     GPDTYGNLFGSVAWDQRTDSGLRVAPGLYIYAIESHTEGTKGKRTIGKLMIIR"
     gene            complement(42646..45513)
                     /locus_tag="PKX09_01225"
     CDS             complement(42646..45513)
                     /locus_tag="PKX09_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU16484.1"
                     /translation="MISKKAYLQIRRLISRTFLGFMLLISLGYAASDGKIVGKVIDKK
                     TGNPLPGVNIIIVGTEMGAATDMEGDYYIINVAPGIYSLQASMIGYKQVIKTEVNVSV
                     NHTTRVDFELEETVLEFGEAVTVVAERPLIELDKTSSRVVVEETDIKSRPVSQLSDIL
                     TSMPGIDYNPYGELTVRMGTLDQVAFMIDGMRARNPLNSQPYFNVNLMAIEELEIITG
                     GFDAEYGEAQSGVFNIITKEGPDHYVGAVEFRWIPPGLYHWGTALYDYSTERYWENTH
                     ARHLQWWIDNPDMWVDPNGRYGNDPSCSWTPEQAYQFYIDTHQPLTDYTNRSGYQTEF
                     SFGGPTPIKNLRFFLSGRYRTAPPITGNAYRKMGSWLDGTYKISYSLGGNKLLVFSGY
                     YNQDNTCHGMESMEYEGDYGNKYAYYDEAGYPVYRRNGQTLKFVHNLDKNTFYELHLS
                     RVFNYESQTKFPSDTADWTTSDIRRDYLRAYDSQGFAIPDAWAEIYGLHYIGYWYRKE
                     DRNTDLTLTGKLTRQINKNWQMRSGFGFTYYTFDRYQEARAWEAVEDKIYHPYEGNIW
                     WSNKLEFEGLIMNLGLRYDFYNPNDVIYLDPYDPFDIITAEMEGREPNPKTKPTYTFG
                     QLSPRIGVSHPITEKSLLHFNYGHFFQRASFGEYGEGEQTYSILSTYIVDPEFGSIEP
                     RNLGNRELRPRKTVAYELGIEQDFGPVLADVTAFYKDRTNDVRSITVITYNEQQYHTS
                     GNSDYADAKGVEFTLRRPLRGFWGGYLTYTWETGIVGQSGDPFTIYPPNVTVHASQDF
                     DIGDQLYYMRPSLSANLTLAAPSNFSPLGGVLANTQFSIDYYVSYPHKQISSDVYQGA
                     VNIIRPANKNANIRVRKEFKGFKGTRPAIFIEMQNAFNDKWHNVDLMEGDISAEDQVR
                     YINSNLMVYPEKMTNGAPFPDYAKYTNLPRKITVGMALSFQ"
     gene            complement(45582..46532)
                     /locus_tag="PKX09_01230"
     CDS             complement(45582..46532)
                     /locus_tag="PKX09_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOU16485.1"
                     /translation="MRIAALYGAHDFRIETKESPDIKDDECLVRVRACGVCHSEIHQW
                     AKTLDNLEYPRYIGHEVSGEILKCGSAVKGFKRGDRVAVWTDRAGYAEEVAVRSNQIF
                     PIADQISFAEAMAEPIACTTNGVIKAGVELGDTVVLVGTGFMGLILMQQIALKGASRI
                     IGVDVRAEILDKAKMLGADLVINPQEEDVPAIIWDMTNGRGADVVFEVGGNQASLDLA
                     VDLLRMEGKLVIFGYHPGQRIIKDLGYWNWMAFNIVNAHFRDMATILRGAEIGIKLLN
                     EQKINMKPLITHKFKLDEIEAAFCAAQEKPKGFIKAVIEP"
     gene            complement(46622..48595)
                     /locus_tag="PKX09_01235"
     CDS             complement(46622..48595)
                     /locus_tag="PKX09_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU16486.1"
                     /translation="MRKVNMCLLALLLLVAVQSVALSQEEIVRLELNVPKVSPADVVI
                     DGVMDESVWGNAAEVNLVTETGYNIWASSYGREIAEPEYDELYGRLLWTDDTLYVFMH
                     IDEFVNDTTDLYWGGQFLDPTDTSGHWASDQLFIGVSNRLGVESWDNWEGNPMAAPDG
                     PYHLMIMGDRVTFNDGYPVYVPELDSAVIFDPSTFIRSAAQWNKETGVWDVELAIYNP
                     NVKASASIGFNVGGSFSSEYSRETTGDSYGYYTWQPNVPNEPFADPGDLGWCATYIQQ
                     NSEYWALLNFLPGPDDVIVRLELNVPKVSPADVVIDGVMDESVWGNAAEVNLVTETGY
                     NIWASSYGREIAEPEYDELYGRLLWTDDTLYVFMHIDEFVNDTTDLYWGGQFLDPTDT
                     SGHWASDQLFIGVSNRLGVESWDNWEGNPMAAPDGPYHLMIMGDRVTFNDGYPVYVPE
                     LDSAVIFDPSTFIRSAAQWNKETGVWDVELAIYNPNVKASASIGFNVGGSFSSEYSRE
                     TTGDSYGYYTWQPNVPNEPFADPGDLGWCATYIQQNSEYWALLNFGTGLNGIDVAEVN
                     EKPVKFALRQNYPNPFNPTTTIRFEVNERVPITLKVYNVLGKEVATLIDNRTYSPGVY
                     SVIWDGSSLSSGIYFYQLVTDKVVSTRKMILMK"
     gene            complement(48709..50010)
                     /locus_tag="PKX09_01240"
     CDS             complement(48709..50010)
                     /locus_tag="PKX09_01240"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOU16487.1"
                     /translation="MRKKIIFWTISMVLILSDFTKAGDASTAPITSAKSLALGGYNYA
                     GLDGITTTFQNPGKILSTPGFGLAMNIIDRAGQQQCQKGSRDIHRSYLFNDFSGSAGI
                     YWVPNQKFGLAMVYNRALDYSVRWPLALMFTENGITRLYGFQLKSRLIADAYSQVFGL
                     QVGKMSLGAAINIYQIYQQFEFPISNPEWQTNRTNPVYALNLEQEDNAIGWSLGFILP
                     ITTTIKVGGSIRSGVDFDLEGNAKTDCFYALDSLAKKSRLHSRFQMPMTGGIGILYKP
                     TDQLNLNIDLSSEFWGSTQSKLDFNYSDTAWSAIIAAAPRDSITGYWRSEMPFYDRNS
                     FVFAIGLEYSGENNLKYRIGYNYQTTPNSDRSYSLLLPNVNQHRISAGIGFLFNQFYL
                     DLSIAYSVGVAKDIKKSQDEYFYGKYDTKTIIPSINVEYQL"
     gene            complement(50044..50883)
                     /locus_tag="PKX09_01245"
     CDS             complement(50044..50883)
                     /locus_tag="PKX09_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16488.1"
                     /translation="MNNIIIEQETFTPKFTWLGGYGCAFGVNRGDQARLDSTLQWLIK
                     VDGDNLHYPVKYGELPEGVTDITNDYGGHFEALIEDSTYTFWVIKEAAWDAVVENAGK
                     VILMAELDTVTTIANEDTLWIDSYSHVQKSCWIDVYTNVDMASIKLRGGFAKITLIET
                     DTSNSPVITWEIIDSSVEDSLVAAVGICLGESYSPKKIVWEAWSVDTVGGQKIYGKNN
                     VIGQPLVIGSPIPGTKVFVEYPAKGLSRNKTYLFWLAGKDWNGVDHLRSTKYHSYITF
                     NTW"
     gene            complement(51070..51912)
                     /locus_tag="PKX09_01250"
     CDS             complement(51070..51912)
                     /locus_tag="PKX09_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009593380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU16489.1"
                     /translation="MAIDFRKNSHIVKDIILHVIIYALALATIAPFIWMLLTSVKDIG
                     DIFVYPPKWLPSKFHFENYARAFQAAPFGRYYLNSLIVACTVTLGQLITCSMAAYAFA
                     RLQFKGRDVLFYIFLGTMMIPYQVTMIPSFMVLYWLGWVDSYQAMIVPGLASAFGTFL
                     LRQFFLTIPKELEEAAYIDGCGKFRVLWQIIIPLSKPALATLAIFTFMGTFNDFIWAL
                     IVVNSDHLRTVQLGLAIFRDRYITDWDLLMAGSVMATLPILIVFFFAQKYFIKGITLS
                     GIKG"
     gene            complement(51912..52844)
                     /locus_tag="PKX09_01255"
     CDS             complement(51912..52844)
                     /locus_tag="PKX09_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU16490.1"
                     /translation="MLNKSTRVKKTILNRRDGNSKKNILTALGFLSPTLVIFCAFVLF
                     PVFFSFYLSFHKWNMFSSGREFLGLENYRRLFSDPEFWMVLKNTFIYTLGTVPLNMVI
                     ALLTAIILNRRIAGKRFLRTAIFTPVVISPVAAALIWRWIYDPNFGLLNYLMDLVGLP
                     SVNWLNEPKAAMVALIIMGVWKTFGTNMVLFSAGLQGIPDHYYEAAEIDGAGWWSKFW
                     NITMPMLSPTTFFIMIMSMISSFQVFDIVYVLTSGGPLGATKVMVFYIYENAFKFFEM
                     GYASAAAYVFFAILAVLTVLQVKFMQTRAGYEAV"
     gene            complement(52834..54225)
                     /locus_tag="PKX09_01260"
     CDS             complement(52834..54225)
                     /locus_tag="PKX09_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU16491.1"
                     /translation="MQFKNIGNQHSPQKCANVFTTNLRGWSRYFLLSFCLLVFIGCGK
                     KETTKDDKITITFWHSFVASTIPALEDLIEQYETENPNVKIKAQYVPTGDALIQKLIT
                     AIQSKTAPDISWIHSDFLADLVDADAIYEMDHFIKSNNGLSKSEIEDIYPALLQYSSL
                     RGKIYCIPMEATNLALIYNVDMVKEAGYQDGRAPSNWEELEEYAVRLSKDINNDGKFD
                     RIGFFLPIFPAAGPLSGWMVWQWIPYLWQAGGYIIAEDQSKVLYNSEAGVKALTLWKN
                     IYDKLNLRTFTTDYDVSFASKLVAMAMDGPWNLPRFNRMLKNLNWSFAPLPKGPVKQA
                     TICGGEYLVIFKQSEHPDEAWSFVKWIIRPDIQARWAMKSGYLPVRRSVSEIPEFKKY
                     LIENPNFKVFVDQMEVAQIQRSMDYGALQITRHMAEAIEEATLGGGDPKAALDKAAAK
                     SNKILQSYWQNAK"
     gene            complement(54251..55285)
                     /locus_tag="PKX09_01265"
     CDS             complement(54251..55285)
                     /locus_tag="PKX09_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU16492.1"
                     /translation="MTATLEDVAKKSGYSVSTVSRVLNNKGKVNRIKDETADKIHKIA
                     QELNYVPNQLARGLRLRKTHAIGLVIPDISNPFFAHVSRIIQIESYKLGYTLIVCNTD
                     ENQDTEIEQIQLLRSKGVDGFIIMPVGLKSDHIQELIDEKIPLVLLDRCFDSIDTNSV
                     VVDNYTGSYKAVEYLIKNGHKRIAIIQGLPNTSTNNMRVKGYKNALIKYNIEIDENLI
                     VGNDYRRENGYIETKLLLKMSNPPTAIFSTSDLITLGTLDAIIEEKLNVPQDVSLISF
                     DDVDFAPYLFTPLTAIQQPRQIMGEMAVKLLYDDLKSKGRKEKTRIELKPVLNIRKSV
                     RNLNSVINPD"
     gene            complement(55837..58251)
                     /gene="leuS"
                     /locus_tag="PKX09_01270"
     CDS             complement(55837..58251)
                     /gene="leuS"
                     /locus_tag="PKX09_01270"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOU16493.1"
                     /translation="MYPFKEIELKWQRYWEDNKTFKTPDKSNKPKYYVLDMFPYPSGA
                     GLHVGHPEGYIASDIIARYMRKKGFNVLHPMGWDAFGLPAENYALQTGTHPKITTERN
                     INTFRRQIKSFGFSYDWDREINTTDPKYYKWTQWIFQQIYKMGLAYLAEVPVNWCPEL
                     KTVLANEEVINGKSERGGYPVIRIPMRQWMLKITAYADRLLEDLDLVDFPESIKEMQR
                     NWIGRSEGADIDFKIEGSDQTIKVFTTRPDTLFGATYMVLAPEHPILKTLVPPEYKKT
                     VEAYQQAAASKSDLDRTELAKEKTGVFTGLYAINPANNRRIPIWTSDYVLMSYGTGAI
                     MAVPGHDQRDWEFAKKFGLEIIEVISGGDITKEAYVGDGILVNSGFLNGLNVAEAKRR
                     IIEWLNEKGIGQSRVQYKLRDWVFSRQRYWGEPFPLYYDEDNNIHLIDESKLPLELPP
                     LEKYEPSGTGESPLANLPEWVDFIDPETGKHCRYDTNTMPQWAGSCWYYLRFIDPHND
                     ETFCDREKEKLWMPVDLYIGGAEHAVLHLLYARFWHKVLYDLGYVSTPEPFMKLVNQG
                     MILGEDGEKMSKSRGNVINPDEIIDTYGADTFRMYEMFMGPLTRVKPWNTSSIDGVFR
                     FLNRVWNLYVTEEGLISPDIKDITPDASTNSILHKTIFKITDDIENLRFNTAISQMMI
                     LVNEIAKLTVKPLSVMKTFLHLLNPFAPHISEHIWNLMGNEQSLAYLDWPTYDPSLII
                     DDLVTMAVQVNGKIRGTFDIERDSEDQVCIAKALQIDNVQRYLEGHTIVKTVVVKNKI
                     VSIVLK"
     gene            complement(58279..59493)
                     /gene="serA"
                     /locus_tag="PKX09_01275"
                     /pseudo
     CDS             complement(58279..59493)
                     /gene="serA"
                     /locus_tag="PKX09_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
     gene            complement(59594..60070)
                     /locus_tag="PKX09_01280"
     CDS             complement(59594..60070)
                     /locus_tag="PKX09_01280"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HOU16494.1"
                     /translation="MIYPACREVKHLLSGEVRTFDCELIFLDEDFGILRYILEKPYVV
                     NNQILPKNTQTYAFYWESQPFTLYRWFAPQGGHLGDYFNIADRIHLSNFQFEWRDLIV
                     DVFISPQGEISLLDENELPADIDANLARYIDQSTQFIIGNHRKILSELNSLTAKLF"
     gene            complement(60082..60642)
                     /locus_tag="PKX09_01285"
     CDS             complement(60082..60642)
                     /locus_tag="PKX09_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOU16495.1"
                     /translation="MNLLALILIAIGLAMDAFAISITCGLAAQPHNKFKFLKVPLAFG
                     GFQALMPLIGWLAGQTLQRFLANFDHWVAFGLLVFIGIRMIYEAFSKKECTNAIDPLN
                     SHRLFGLSLATSIDALAVGITFAFLEINIVVPIIIIGVITFLISLGGIIIGKRIGHYL
                     GKKVEVVGGIILIAIGFKILIEHLVA"
     gene            complement(60643..61965)
                     /locus_tag="PKX09_01290"
     CDS             complement(60643..61965)
                     /locus_tag="PKX09_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009606163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOU16496.1"
                     /translation="MNTDLFIIIGGLVIISLLVIIMAKVRKKTDQSGDAALEALKEKI
                     IALESQLNIFPELVQSKTADIVQKRFTEFTDNVSTKNREMIEKFGNFQVEITKSLSEN
                     SKKQTEEFSQFKDTFKRAVSEDFEHLTKNVAEKLDAINNKVQENLSEGFKKTNETFTN
                     VIERLAKIDEAQKNIESLSTNVVSLQEILSDKKSRGIFGEVQLNQVLYAIFGEKNDKI
                     FQIQYTLSNDKTVDAILFLPEPIGNIPIDAKFTLDNYKRSIDKSLSEAERITATKLFK
                     ADVKKQIDEIADKYILPGETSNQAIMFIPAEAVFAELYAYHDDLIEYSHSRRVWLTSP
                     TTFMAVLNTVQLVLRDIERRKYADIIQQEIMKLSEEFRRYRDRWNKLSAHIDTVQKDV
                     KDIHTTSNKISNSFEKIAKVELGEKSELPPEQEVKYLNVINEEESEQE"
     gene            complement(61978..62451)
                     /gene="tadA"
                     /locus_tag="PKX09_01295"
     CDS             complement(61978..62451)
                     /gene="tadA"
                     /locus_tag="PKX09_01295"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOU16497.1"
                     /translation="MGLIVNSDEYWMDFAIKEAEKALDAGEIPVGAIVVHADKIIGKG
                     HNQREGLKDPTAHAEMLAITSAATFLDSWQLGDCTLYVTLEPCPMCAGAILNARISRV
                     VFGAYDNAAGMCGSVENLCDQNLLNHKALVRGGVKAELCRSLLNSFFNKIRSTEN"
     gene            62877..63665
                     /locus_tag="PKX09_01300"
     CDS             62877..63665
                     /locus_tag="PKX09_01300"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOU16498.1"
                     /translation="MDDLQLQQKLSATALNAALEAGKILKQKVNTALKIDYKGRVNLI
                     TEADRMSENSIIEAIRRQFPEHQILAEESPLIETSSDYKWIIDPLDGTTNFVHGFAFF
                     SVSIGLEYQKELLLGVVHAPLLGETFTAMKNQGAFLNGQPIRVSRVRKLADSLLATGF
                     PYENNATFRRNFEHFRRIYPLTQGVRRVGSAAIDLCYTACGRFDGFWELDLNAWDVAA
                     GALILQEAGGKTVNMDGSAFSIYARQILATNGLIEAELLPIFKE"
     gene            complement(63669..64922)
                     /locus_tag="PKX09_01305"
     CDS             complement(63669..64922)
                     /locus_tag="PKX09_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOU16499.1"
                     /translation="MIPTGLAILIIAICVLGEGFFSGSEIALVSVDRIRLRHDAKMGK
                     KDAKLVINLLKRPEWVLGTTLIGTNICTVTSTMLATAIAYRYFHGAGVFVAIFCMSLI
                     NWIFAEIVPKSVFQQYADVIAPKIAPVLRIMSLIFTPLVRVFSGAASYIATILGGELS
                     HKLPFISKEELKILMKISDKSDVNPNEREMIGRLLSFKEKKAEDVMVPLIQVTALPED
                     DSVPEAARIISESKHRRLPVYRERVDNIIGILNSFDVIGENNNTPIRRFIRPAFYAPP
                     NMSINVLFDQMKKSGNNMAIIVDEYGGAEGIVTIEDILEEVVGKLEDEYDHVRPRYKI
                     KKDGTIVVSGKMEIQELNDKFGLNLPEGDYDTIGGLIIAHLQKIPRPGERLILPSVAL
                     TVTRASQRVIHEVAIRKNGSTEVTN"
     gene            complement(64919..66178)
                     /locus_tag="PKX09_01310"
     CDS             complement(64919..66178)
                     /locus_tag="PKX09_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOU16500.1"
                     /translation="MVLVTIEIIILVVLLGLSGFFSSSETALFSLSKIQREKIQESDP
                     AQGKIINDLLNTPRSLIITILLGNEFVNICFSSLFTLIVIQIAGTVLPWANYLIALPL
                     VLLIGEITPKTLAIKNNERLSLKFARPLSLVMVIFTPLRWFIRNISDRIVNLIVSKRD
                     QRENILTEDVIRSILEDSEKEGVIDSDESEMISNVFDFGDLVAADVMTPRSLIFSLPV
                     DMALPEVITAIKNRRISKVPIYEGNPDNIIGVLFATDLIGISLNASNDETKPINLRNL
                     LRPPFFVPKTKRVDELFHIFQRKRISMAIVRDEDGIVQGLITIEDLLEEIFGEISDEF
                     EIKDEQFEKINDRLYRVRGTLPIVSFNELLNARIPVDEFETIGGFVSSLFDELPTVNA
                     QKIYDGWLFTVEKIENNRIEILRVKRL"
     gene            complement(66244..67737)
                     /locus_tag="PKX09_01315"
     CDS             complement(66244..67737)
                     /locus_tag="PKX09_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOU16501.1"
                     /translation="MFNITDLIVVVTSVFCLLATAESQEYSGEKYKLDAHKDTIWTKG
                     IPVEYSDFPEAEKIAIAVMELDANGVTLAEAKALTDRLRIELFNAGIFQVMERDKMNK
                     ILDEMQFQMSGCTSDECVVEVGKIVGVKKIIAGSVSKVGEIYSVSIRLIDVETSRIEA
                     TAIEDIEGALGAVLTEAIPSVARQISGLERQTKELKGKKTAFKISTEPYGASVFIDDV
                     YYGIAPIEVEVVPSVTHRLTIVNEGYDKWEHYYDIKKDQVLEINVALSKKLQPVTKNV
                     AEKPKRQFKTGFKIRYSDFHIVDKINNQVQIMNAKMAEGNELFHRRQPGAYVFDEINN
                     FSGIELSNLRQSDEVVGFDFGLGIYRSSFTQWIKNFDATYSEPTLIFWMPSVNLNLRL
                     APIRFPLFYPFCNVGFGYDVLIIDAVEDNKSMGSPIFHSWGLMYGVGAELRPFKFIGL
                     ACDWNHYNMKYKLMNIENKATQRFKDASISHIDLSGNTVGLSLNLYY"
     gene            complement(67960..69246)
                     /locus_tag="PKX09_01320"
     CDS             complement(67960..69246)
                     /locus_tag="PKX09_01320"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HOU16502.1"
                     /translation="MKKLLLILMLTIYFVLFTQRIEAQNELWDRIFVFPENYRIYTLS
                     FSDSLNGWAGGETIGPVGGVILNTKDGGQTWSEQFVYREFSWFNNIDFVDSLNGWAAG
                     LHCLFKTSNGGKEWIRVADSLFDFDIYMGLSDLQSVSFADTAFGIITGTESTIAITLD
                     GGNTWNCATINPENVFIDTVYYGVVLSAGKACVTGRGGVAVSADSGKTWHITHNEDRS
                     YIKCSFIENGYGWVLSRDGQIQKTRDYGESWIFCKQIFEDGVDYAVDISFLDSLTGWV
                     IANNGEIWETNDGGYNWLSSTISQDTALAVITSATNNATFVLNKANSLYKYKRFSAIT
                     EFFKKLPDCYKLCHNYPNPFNSSTNIKYSIPTKSPVKIQVIDVNGNLLETLVDRSHEP
                     GNYQITWDASRFSSGIYFYRIQAGDFIEARKCLLIK"
     assembly_gap    69455..69554
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(69816..70571)
                     /locus_tag="PKX09_01325"
     CDS             complement(69816..70571)
                     /locus_tag="PKX09_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOU16503.1"
                     /translation="MHLTILGSGSFLPNEKRNCSGYLLDIGDETLLLDGGSGTLRQIV
                     KAERSVLEVGRVFYSHFHLDHIADFLPLLFTRKYYKPQRPNAPLYIHAHPDFAYYFNN
                     LTKMFEKWTVDPDYPAYFSPITPGMHDFSSYQVQVFPSNHAPESQMYRFIGPDGESLL
                     YTGDVGLDQILIDASRNVGLLLIECGNLSNVPIAGHLNPEQIKTVIEVAQPQQVILTH
                     LPPEVDNLDITERFGPSLAKRIILAEDFFTFDF"
     gene            70708..>71255
                     /locus_tag="PKX09_01330"
     CDS             70708..>71255
                     /locus_tag="PKX09_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOU16504.1"
                     /translation="MNKNNTGEIIQAMPKIELHLHLEGAFPLESLLRLIQKYGSNEVH
                     NIEELRQKFVFTDFAHFIETWYWKSDFYREPSDIEDMAYSTIASLSLQNIIYAEVYFS
                     PFDFIKPQMPFPVVTEAVISGVRRAESEYPIRIGLIADIVRNHGHETALARLDKITQY
                     QNEVLGIGLGGSEKEFPAKLFA"
     assembly_gap    71256..71355
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <71356..71595
                     /locus_tag="PKX09_01335"
     CDS             <71356..71595
                     /locus_tag="PKX09_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOU16505.1"
                     /translation="VRRLFDYGLLITINSDDPTMFGSNLTDEFNFIANEYNFTLPEIK
                     KLTQNAIQASFAEDSFKQQLTLKNNEYWNSIGVKE"
     regulatory      71703..71899
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            71929..73995
                     /locus_tag="PKX09_01340"
     CDS             71929..73995
                     /locus_tag="PKX09_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU16506.1"
                     /translation="MYHSLLKIYRFFPLWLLPIALEAMEFSSGFICDQNNQPIPYVFI
                     YNKTKDSFIVSDENGIFILPASYQAGDSLLFTRIGYGEQEIVLGNLYEPVKIILPFTP
                     VNLDSVTVAGQIFPRQLSGINFSRVERTEDLGTVEHRRLWTTIPGLYLKSYGGPTGIS
                     TLSLDGSPSTHTRVVIAGFDLTSAQNGQMDISQLPPTFISSINYSPEQAGNTSAENSE
                     GLLNIEPNMSPTGFTCSAGSYGKLNLSANLDIRKTVWNSNILLGHNYYRGDYEVTWRD
                     QNFKRSNNSLQQNYFSWQSGYVFRSKAFARLIAFYSEQERGVAGQVWNPSPDAFRQDA
                     LGFIGLKIGWAVKAGHSYIQTLHRHSDERYVNPTIAINSRHVVNTDQIIYHANRSILN
                     FNLDFTSDLKSDRLQSKQAGHHSRITLANILALPWNFHPNYELRPWVKFEHSPDLYQK
                     ATWGGRIAYRGSGILNTISGSYGQYYAYPTFNDLYWQPGGNPNLKPDETTKYEIDLIL
                     QILPELKLVLNSYYKKDKNLIQWTPVQSYWQPSNISQAERKGIKVILNWSLPNLPVDG
                     FLQGVVTHSCNLTKGNNYQKPLRYTPKESYAAGLNFNFKHIVFHSTIEYVGARIAMYN
                     WPEDIILAEYLVLNANLAYKVNTTIGRFTIVAGCDNITNKSFESMQGYPEPGRAFTGT
                     IEYNPK"
     gene            74013..>75064
                     /locus_tag="PKX09_01345"
     CDS             74013..>75064
                     /locus_tag="PKX09_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16507.1"
                     /translation="MKQKLTLLAIVTIFQISLAQNFTYILCEGNFNTINASLWQLTGT
                     SELIGPVHWNFADNPLGDVGQNLKVYQEKLFIVMNNSNSLEVMSLSNGATYERTIDLP
                     FAGPRDIAFVNNHAYITCWYLPGILSLDLNTWQIDDTIFINGLPDNIIADDGKLYTSI
                     AMNTDWSPADKVLALGLISGEFTPVDTFTVITGPNQLLLYENKLYVLSNYYDDAWNTY
                     TGTSCINLETNQVTKRDQGLSFNFCDDLALINDKIYRSYNGGVAPLTDSLTIDINKII
                     GNYAGIYSMAFDGKNIFIGLSDYSAPDQVVILDTLGNEINTLNVGVCPGSFAFYQQPN
                     AEIFTDEKNGILSDFLL"
BASE COUNT        22255 a        17246 c        13536 g        21827 t
ORIGIN      
        1 tcgaataaaa agacttttgg tttccggaca attgcccgac cgagagctac tcgctgacgt
       61 tgaccgcccg aaagttgttt cggtttccga tttaataaat cgcctaactc caaaatatca
      121 gccgcttcct taacccgctg tcggatttcc gatttaggat atttacgcag tttcaagccg
      181 aacgccatat tctcgtaaac cgtcatatga gggtaaagcg catagttctg gaaaaccatc
      241 gcaatatcgc gatctttggg gagaatatta ttaaccagac gattgtcaat ataaacttca
      301 cctctggtta cttcttccag accggcgatc aagcgcaaag tggtagattt gccacatccg
      361 gaaggtccga caatcactaa aaactcacca tcattaatcg tcaaatcgag atttttaatg
      421 attggagcac ctttatcaaa ttcctttaca atttgtttta attctataga agccaacgtt
      481 ttccctttct cgttttataa attaactaac gttcaaattt actacacaat atttcacaat
      541 gttcctttga ctattaatcc cgattacatt ttcaagtaaa attttacact tcgttaatat
      601 tcttgtccga ttgtaattta aatttcgata aatactcagc cagacgttca cctaagcgca
      661 agcaggcggc tttctccgcg gctgaacgcg gttcaccggc cacagtcgct ccataatgaa
      721 gagtcaactg ttcatctacg tagtcggtaa cgccgaaaac caggaatccg aaattgatga
      781 gaatcatcaa agccgattgg caggtaatct cggcgccacc accccaacct ccagatgacg
      841 aaaatgcgca accgattttg ccttcaattt ttccccagag ttcatcacct aagtcatccc
      901 aaaatttttt cattttccag gatataagtc ccatattagt cggtgatccg agggcaatgc
      961 catcacacca acgaatatct tccgcaccag cattgtcaat atgtaaaatc ctgacattgt
     1021 gcttaccatt tttccgggca ccgagggcta ccaactcagc catcttagcc gtattaccgg
     1081 ttttagaatc atacagtatt agaatattgc tcatatattt ctcccgattt taagttaatt
     1141 tctataaagt aactttcaga ttcatcaacc tttcctttta ctccgtttcc gatgataata
     1201 ggcgtctgat tttctcatca gccactttca gcgatttttc tggagctatt ctaccaatga
     1261 tactactttc cagcgcttcg gcgatacatt gattgacttc aacccgaaat ttatccggca
     1321 gactacgtcc tcgagcatac cgaatctgtt cattgaaaga tttttgctga ggaaattgtt
     1381 ccaaaaaatt aacataatca gaatcattca atactgaacg tcgaactggc agatagccag
     1441 atttttcagc aaaagttcgt tgaacttccg gcttgagaaa ccaattgata aaacgccaag
     1501 cttcctctgg attgatacat tgtttaaaaa cgaccagact ttcacccgcc aagtaacttg
     1561 cccgtcccgc tgaaccggca ggtagcggcg ccacgcccca atcaaattta acatattgac
     1621 gatagcgtgg taaattccaa ggtccatcca taatcatcgc taccgtctga cttataaaac
     1681 ccatatcgtg tgccatagaa aagcgctcga atttttgttg atgataaagt ttactccaaa
     1741 aggcaagcgc cgcaacgcct gcctcgctgg caaatgttgg ttgattatta ctaaaaatct
     1801 cgccacccgc ttgccaaaca aaaggcgccc actgcatcgt catccagata ttgagcggac
     1861 cagaagccgg aaaaacagga acataaaatc cgtagcgctc cggctgtcca tcactattag
     1921 aatcgctaca cagttgcatt gtaaaactgt ccagttccgc ccatgtcgcg gggggatgat
     1981 cgggatttaa accgatctgt cgaaaccaat cgcgattgta caaaagggct aacgtcgttg
     2041 cttccatagg cagagcaaat aaagtatccc gaaaggttat cggcggtaat agctgaggaa
     2101 atatatcggg aagacactgg ttgtaaaata atgaatcgtc accagcgagt tcatttatcg
     2161 agaaaattgc tcgactatta accaaattat ccagaaaatc ggaatgtatc caggcaatat
     2221 caggtgccgt ttggctttga atcgccgtaa taagtttttg caccagcgca tctccggtcg
     2281 gaacatactg ggcttgaatt ttgacacctg gattttcttc ttcaaaatca gcaatcattg
     2341 cctccaatat cggtaaagta gtagccacga agctatgcca aaaggtcaat gtaatgctat
     2401 tggcatcttt cggtttcggc ttggaacaat tattagccat aacaatcaaa aaaacaaaac
     2461 cgacgataga caatctttta atttttctat ttttcacgat acaaatgccc cggacgtttt
     2521 ttgcctttca tactgccttc aaaccagaca gccagaatgg gttcttggac aattggaaag
     2581 tcgtgatgtt caccggctgg ggtaattacg caatccccag cttgaacatt ataacttttg
     2641 ccttccgata ctgcgatgcc cttgcctttg acaatgaacc agtattcatc acaatcgtgg
     2701 aaatgattat caaatacggt gtttcgttca tactcgaccg gatcgccgtc gttacgcgga
     2761 tgctcacttt ttcccaaccg gaagacccca caactccccc gatcgttact ccaattgcca
     2821 ctaatttgaa acaattctac cagcggtttg ggttcaatcc acacctcacc aaaaaattga
     2881 tccaaaacat cccctgaaaa tatcgtcctt ttttcggctc cttcgctaat ttcagccaca
     2941 ccatccaaaa cgaacagcaa tgtttcggaa aactctgatg ggagctgtag tttttgcctt
     3001 ttagacaaac cgattatttt acagcttttg acttccgacc aatccggaaa cggttcattt
     3061 ttataggata ttacaggcat taggactttt tgggtattta gttaacatcg gaacatccgg
     3121 gtttatatcc ggcccgaaaa atttatagat aattaaatcg cggtcaccgg tattgacgac
     3181 ttcgatcggg taaatagccc gctgatggca aatcaacagt tcatcttttt caaaattaag
     3241 accctctatt tcgacatttc cgtaagttcc ctgaccccgc caagccaaaa tattatacac
     3301 tcctttctca actgaaacgt gttttccccc cggacggata atcagcttct taccgctgaa
     3361 tttattagta ttataaaaaa tccaggactg ttcacttcca gattgctggt cagaatcaat
     3421 gagtattggt ggtgtatgcc ggttttcata aaagtaaggg tcgccattta attcccaatc
     3481 aatcattccc agaatagctt tttccgccaa atttttccgt tctttttctg aaacatcctt
     3541 aaatagtaat tctttatcaa taataatatc gccacctact ttagcctgca tcattgcgaa
     3601 cacatcggaa tcttcctgta attcaatagt caaggccgag ccaggtgcgt gtagaattcc
     3661 cgccggaaca tgaaatccat cgcctgcctt taaacggtag gcacgggaat ggagaagaat
     3721 gctatcatct ttccactcct gcatagaagg taaaagtaat tcatagcgct tttccagact
     3781 aatataagga tgaacaccaa aataggtttc cggctcaggt cccagcggta cctcttcggg
     3841 gaaataatag gcttcttcct tactattgcg accgactttg cgagcatctt ttgccatctg
     3901 gtgtaaatga taaggcaagc gataagcata gtcgaaaatt ttcggcaagc gtcccagccc
     3961 ggtatgagcc ttagcataat cagctcccat aatcaatgcc ggggctatct caactgccgc
     4021 ttttagagta atttcctgac catcttcaag atccaaacag cataatcctt cattgggtac
     4081 cttaatacga ttatcagccg gtgtagtcga acctacccag cgttcgcaaa taccgccgcg
     4141 ctcaccgaga tcatacattt cctccggcaa tcccaagcgc ctacccggcg gtaaaaaatc
     4201 cctagcaacc caagccggtt taagacgcaa aataccgtca ttatgttcaa ttagcttttc
     4261 taattttgaa ataatttcat tcataaatta acctctaaat aattattatt tccaatttat
     4321 cgttggttga caatcctcgc tttgaagata agcaactaac tcatctctta atgtcgtcgt
     4381 aaaaggatca tctccataat tctgaattag attaaaggtc gttaggatca atttccctga
     4441 gccaacccgc atttgcatta aataagcgga attcaaatgg agccagccga catacattcc
     4501 ggctaataca tcaacaaact gctccggttg aatcccggtg atggcatatt taggtaatgc
     4561 cttataggct tcaaagccca gcactttatt actttttaaa ttattaaatg gcctgtgact
     4621 atcgtcaacc caattcaaat tcgaagccca attgccgtca tacccatccg attcccgtga
     4681 cgtaattgta aaaggaaaat tggccggtaa aactgagcta gaatcaatga cgcatattac
     4741 atccaagcca tttttcaaat ggtccattgt ttttagatca atagaattac atattaaaag
     4801 attagaattc ggcgaaatat tctctatctt tcgcgacgga tataccgtaa actcgataaa
     4861 attctccgct aagacatccc cttccctgct caataaacgg aatttgacac gttgagatac
     4921 cggttgtttt accggctctg aattaatttt catacttccg acaaaatata caccgaccga
     4981 atcaatcgca atactcttgt caccgataat ctcaccattg taaatcccct ggatacgaac
     5041 attagaaaaa tctctattac tataatgtga aatagataat tgcacgtcta tctcttcatt
     5101 atcccaaaat ccatacttgg ttagtcgagg aattataatg tcaggctgtt gaacttgggc
     5161 aagtatctgg cgatcggctt taaaattgcg ccacatatcc aacaaaccgt tgcattccca
     5221 gttaatgtcg gtaaattcag tgattacgta accttgcaat tcaggttgga gccgaatttc
     5281 ttcaatttcc cacttcaaca cctgtgcttg agctttctga ctgaattcgg ctaattcgtt
     5341 atagtttttg aacattcgtc cgtactgata atcattaaat cgttggatga ctccctccgg
     5401 caatgttacc agattatcca aaaattggcg gtcgaaccag aacggtaatt tctccggcag
     5461 tctgggcaag ccccaattgc cgaattccga gatcatcaaa acttcctgcc cggtttcttc
     5521 cgcatcgcca tagggactaa aaagccaccg tgggcggtcg gcaacttcct ggactgtttc
     5581 gctgaacttc tggtgatttt cgggaattgc ccaataggta tggtagtcat taatatcggt
     5641 tttcaggtga tagttacccc agcaggcgct attatcaact attagccgac cattggcaag
     5701 ggatttagca aagtcaaatt cagctttgag ccattgccgc tgattggctt gattaaggtc
     5761 gagtccccaa ctttcgttaa tcagactaat aattgccagc gatgggtgat tccaatcgcg
     5821 ctgaagcatt tcccgcaatg tttctcggga acgggctttg acgctctcat cgaagacatc
     5881 ccagttggga atttcatacc agactaataa acccaattca tccgccacct ttagataacg
     5941 cggatcagga acttttatat gacaccgaag agtatttaat cccagttctc tggctttgtg
     6001 catttcatcg tgtaaatact cttcagacgg cgtttcataa acggtttccg gatagaaatc
     6061 ctgatccagc gcggcgatca tataaaaagg tttaccatta agataaaatt ttccatcacg
     6121 cactacaatt tccctaaaac cgaaacgatc gaacagaaca tcttcaccca aacggatttc
     6181 gagagtataa aggtttggtt gaccgatagt ccacaatcgg ggattattga tctgccccgt
     6241 aaattctgct tcggaagtcg aagagttccc ctcatattta tcagaaaaaa cgaccttgcc
     6301 attcgggtcg cggattgtta gaattaactc gacaggagta gcaccagccc tagcattttc
     6361 aataaaaacc tttgcttgaa acgcaccgct aatttgagaa ttaacctgaa tacgtgacac
     6421 ataaatttcc ggtttgatag ccatctggac tgcttgccag atgccgctgg tttggacata
     6481 ccagttttgt ttgccgtgcg gtatatgcca gtagctaata ccatcggtac cgactttgtc
     6541 agccacagga tcaacgactc gcactaggat ttcattttct cccttttgca attgctcggt
     6601 aacgtcaaaa ctaaatggcg tataaccacc ttcgtgttca ccgactaatt cccgattgac
     6661 atatacctta gcgaggtaat ccactgctcc gaagtttagc agaaagcgat gtttgggagg
     6721 aagcgatttt aatttgaaca acttgcgata ccagacaact ccctgataat ctaacaagtc
     6781 cacatgcaag cgatgccaag aacccggtat ttcggtcatg cgccaatttt taatctgatc
     6841 aatcgaatta atcgtgattt gtccggtgct atccggcgct aattgccacc gaccgttcaa
     6901 atcaatatcc tgggtcttat cgcaggaatt aatcataatt aatcccgcta tcaatactgc
     6961 taataaattc catcggctca tattaaaaat tttagttatt tgattaatgc tatttactcc
     7021 aactacgact aaaagttagc tctaaaactc gattggaatg cttttccgac atacactaaa
     7081 ctttccatct tatttcaata acaacatttt cccgaccaga atttggtctt tatatttcaa
     7141 acgataaaaa tagcaaccgc tcgccaattg atcagttcgg attagtacct gatgttcacc
     7201 gggattttgt tcaccgtcca ccaattccat cacctctcgc cctaacaaat ccagaacggt
     7261 gagtttgaca ttactctttt gaggaatcgt atatctaatc gtcgttgaag aattgaaggg
     7321 attaggataa ttttggaaaa gtgtgaattt ttcagcaatt gctgggtgct cttccggtgc
     7381 aatattaacg ttgaccattg aatcagctaa taaaaacaaa gaggtcgtat agccgggaat
     7441 agtcagagcc acactactta gattttccgg tttcccagaa aaggtcattc ccgtaattaa
     7501 atctgaaagg taataggttt tcgtagaatc aagatgcaat tcctcaatcg gcaaggagat
     7561 ggtaatattt ttaggctcac tcatcatatt gataacgctg aacaaattct catctccact
     7621 acgacgatgg aacgcgaaag catagtaagc cgggactacc gggatttctt taaaataatc
     7681 actgcggagc gccgaatact tttgcctgat ttgaattaaa tggcgataat gatcgaaaag
     7741 tccatattta tcctgactac ggattgattt gcctcgttga aaaatctggt aactactata
     7801 aggatgttta tcgtagccga tttcctgacc attataaagc atcggcactc cattcaaagc
     7861 aaaaatcaga gtcgaaacca ttttcgtctg agccagatta tgatttttaa taaagggaat
     7921 catatcatta ttgcccatat agcgcaaaat cttggcatta gccgcatagc cttgcccatt
     7981 attcgtcaat gcctgacgta aaaggttaac tctttccgat ggattactgt aattaaaaat
     8041 cgtaaattgt ttggtggcgt cttccgaata gtcaacttcc caataccact gcgaaaccca
     8101 accttcgcct ggcgcccaat caaaggcgac atcaaaacgt tgatcaaaaa ccattgacca
     8161 ggaagcttta tcttccgcca gtagcattag ctccggcttg atgcgcttta attccaatcg
     8221 taagttttgg gtaaattctg ggcaacgtgc atttacaccc cacactgcat caaagcgata
     8281 accgtcaata tcaaattttt ctacccaata tttacaggct gtcatcatcc agttgcggac
     8341 ttcgggatta tcataattca agttgggcaa ttcatcccag aaataattga taaacccctt
     8401 ataataatta taatattgag aatagggtgc gttgtctatt tccctttgat aataattgta
     8461 ataatgggaa gcagtaccat atttcagact atgttgagca taaggatgat aaatagacgt
     8521 gtgattgatg acaatatcca aaagaacttt catccccagc gtgtgagcgg acttgatcaa
     8581 attctgcaag tcactttccg aaccataatc cgaacgtatg gtaaaatagt caattacgtc
     8641 gtagccttgc tcatcccact gagttttgaa aatcggctga agccaaatgg tattaatccc
     8701 caattcccgc aattcgggca gtttctgttg aatatcttga aaggtacctt ccataacaaa
     8761 ggcgtaagga ctaatttcat acaccacggc cgaatcaatc caagccgcat gatcgcggta
     8821 aatatcaaac ggtttaatac catcaaaact tactgtgata aaggtgcgag cacgagtagt
     8881 gtctccactg gtagtaaccc cgatcaaatc gaaataatat tcaccggcgg gagcctgctc
     8941 tggtacgtta aaaaccaaaa ctgaatcgct cgtcccattc cagccgaata tagcgggatt
     9001 gcgctcatct tcagaccagg taaatgtaac cgcatttgaa tcgggatttt cgatgatttt
     9061 accgtgtaat gtcacttcgc ggtcagcgac cgtcgcatag gcttccagaa ttggttttaa
     9121 ggacattttc atctgtaagc gtaatgtgtc agaataaatt ggttcacctt gattttcaat
     9181 cgttgctaaa aaccaattct caccctcatc taattttact tcgatgtaaa agctggaatc
     9241 ggaaggatta atatcgaata ttttacttcc atctttccaa tataaaatcc cagatgtagc
     9301 agtcagtgta tcaatttttc cccaaattac ttgttccggt tgccatacct cgatattatg
     9361 agtttgccac tggagttcta ctgctaacgt cgaagccaag ctaagcccaa tcagtgtaat
     9421 tattttaact atattccgca ttgcaatgca cttctggact tttttagcgt actaatattt
     9481 tccatacgtt ttagctactt ccaccatctt aaatatattg gcatctgggg tatcacgtcc
     9541 acactgatca ccggttgaaa gaacaaaacc accgtcatag ccgacatcat caattagccg
     9601 tttacactta tcttcgacct gttgcggtgt cgctcgcatc ataaattcgg ttgtattaat
     9661 attgccttta atattcaatt tccgtccata tcttttctta accatagcaa tatccacatt
     9721 gccggtcggc ggttcttcca tcggttccat cgaagtaacg tccgtttctt ctgccactat
     9781 ttgcaccaat ggccaggaac gaccacaaat atgcaagtgg caaggcatgt tcgccgcttt
     9841 acaaatgctg gaagcctgct gaataaaagg taactcatat ttccggaaaa tatccggaga
     9901 acttacgcta agactagcac tggagccgcc caacataatc acatccggtt tagccgcgat
     9961 catcgcccgc aggtaggcta atgcccaagt catccagtat tcatgaactt tttgcatata
    10021 gtccgcttcc attaataaat cggagaatac attctcaaag ccaccatccc gattactaaa
    10081 ccaccaatcc tgaaaaaccg gtatcattcc caaatagact cccagatcac caatcacctg
    10141 tttatcacga aattcctttt cccattgcca atcacctttc cccattacca actggaattt
    10201 ctcgaaatcc tcttgcaagt ttttaatcgg tttgacactt cgccaagggg attcatcggc
    10261 aaaaaatatt tcttcttcgg ttacatcacc cttggctgta tgccaggtgc gtttaacgag
    10321 tttgccagcg ggtatatcta ttgtttccga ttcaaaattc agtgaactcg atactttcac
    10381 ctctttcaag ccgccataca tataaaagcc atccatatcg aaatatttta cagctttgac
    10441 ataaacttca ccgtgggtgg ctgaagtcca gccataatgt tcacgaccat ccaaatggat
    10501 ttcataaaat gggcgcccgt ctagtctgtc cggcaccatc accgatatat ccggactaac
    10561 cggaacgtgg tctgctactc caccttttaa agcaatcaat aagcgttctt tcgatgtcat
    10621 tattttcgtc ctatttttta atattatcag gcaaacgatt ccagctcctc aataattcct
    10681 tcaagcgata aaacgactgc ttcggttcgc aactttcggt caccagcccg ccattgacaa
    10741 taaacgggcg aaaattggaa aaatcatacc agttgatggc tttaatattg agcctgctgt
    10801 aataaagagt ataaacttgc tccaaccaat cagcctgtaa ttcttcgtcc caggcccgat
    10861 gccaatcata gggtttaccg gatataccca aactgcggtc cacaacggtt tcaaaagtcg
    10921 gacctgaggt agtgccgatt tcagtgatat atatcggttt cccaaatttc tcaaaccttt
    10981 ccagcatgcg cattatatcc gataaatcgc gattcccgaa gtagatttga attcccagcg
    11041 tatcaaattc cacgcctgca tccagcaaat cggtgatgaa ttgataaggg gtgcgcaaag
    11101 gccgggttgc ttccatccgt gccattctac ctctggcaac gtactcaccc cagggtgcac
    11161 aattattgat caggcgatct actttaggat taatctcatg ggttttatca catacttgtc
    11221 gcacaatttc ggtgatttga tcgggagtat gattgaaaat attcgcccaa tcatgatatt
    11281 cgtttatgac ttcccaatgc aaaacctgat cgccataatg accgacaacg tcgctaatat
    11341 gtttatctac ataacttctc aattgatcat aatttttact tttcaaccat tccggagtta
    11401 cagtaggatg aaaccaaaag agtggacggc cttctatagt aatatttttc tcggttagcc
    11461 agcgaacgat gttatccttg atcccccagt tataaactcc ttccctcggt tcaaataatt
    11521 cataccaggt gtcccagata taatgagtta ctgtagcaaa attgaataat tcgacaaaac
    11581 gcttggtgaa ttcttcattt ttcgcccaaa catactgacg ggattcacag ccaaagtaaa
    11641 cttcatcatg gcgattattt ttagaaatca tttggcgggc atgttccaat tcgatattct
    11701 ctcccgtcca taaagcatag taaagagctt tatccgctat agaggcacac ttttcacttt
    11761 cggcggtata tttagcggaa tcttccagat attcctctgc taaaccagac aaacgaatta
    11821 cttccggtga aaatttagta ccctgagttt gatagcgtcg cataacttgg cgatttcggg
    11881 ctattcggct tctggcaaat tcgtgattca agataagttc atttcgtccc gtcatttcct
    11941 ttaaagaata aaattctccg tcattatcgg cacttaaaaa acagtttccg aagccttcga
    12001 taaaccactg gcaattcaca ctgaagcgtc gggttcccaa atcctccggc acaacaatcc
    12061 catctccatt taccacgata ccagattgaa aagggtcgcc attggtgtca gccgcataaa
    12121 caaaattaag cggtggcatc cacggatgcg gaaaaggttt aaataacaat ggcttagtag
    12181 ttttggcaag aatattcgat ttttccagaa aagacgggaa catcgcggct ccaactatac
    12241 ccattgtact tttccgaatg aactcgcgtc gtgataaagt tttcttcaag gatggatcat
    12301 tattcattta ttgtaaaatc ctccatttct cttgtaattt tttcaagcga tcgacggccg
    12361 ctttccgttc acctttcggt gaacgcaata agccaccatt tttcaagaag gaaaatccat
    12421 caatcaaatc ataccagttt gccgcctgaa tcattggctg actataagca taggtaaaaa
    12481 tagcttccag ccagtcggct tgaagttctt cgtcccagtg acgatgccat tcatagggta
    12541 aagttgaata atcaccaggc tcttcatcca gaaattcttg cgtgattcct ctggacggtg
    12601 ctccgacttc cgccaaatga atcgctttac cgagcttttc atagcgttcg atgacgccga
    12661 tataatcggc taatgtcttt gaggtaaaat agacctgcac accgattgca tcaaaatcgg
    12721 taccagcttc gatgatttgc tcggtaaatt ggaatggcgt tcgctgaggg tatcgagccg
    12781 gaagctcatg ccacaagcct ttctggacat attcaccaaa gggactacaa ttattgatta
    12841 atattttcaa attgggattg acatcgcgaa caacatcaca ggcaaactta gtcaattcaa
    12901 tcgtctgctc atggttcaaa aatagttcat tcgcccagtc atgtaactca ttgaccacct
    12961 cccatacttt tatcctattt ccatagtggc caacgactgt ccgaatatgc ttttccagat
    13021 aattcagcaa ttgaggaaaa gtcttatttt tcaaccaatc cggtgttacc catttatgtg
    13081 tccaaaataa cggacgacct gcaacctgaa tcttgtactt ttctaaatcg gctaagagac
    13141 ggtcacgttc ggcaaattgc ttctgccctt cgactggctc gaaatcaacg aaatcaccaa
    13201 tcaggtaatg agtaattgtc gcataattaa atacttcggt aaatcgttcc agaaataatt
    13261 cactatccat ctgaaaataa ccacgcccat cacaaccaaa taggaaatcg agccgggcac
    13321 tatttttagc aatattccag cgcgctttct ccaattccaa catttcactt gcccaaaggg
    13381 cataaaggag agcttgttgg gaaaatcgag cgcaggtctc atcatcctgg acatttttga
    13441 tcgcatcatc taaataattt tccgccagat taatcatagc agacaattcg cgagatgcca
    13501 accaaccttc acggttaaaa tttgcttgcc gacgccgatt acgatagatc cgggtttttg
    13561 ctaattcaaa atttaaatta aggtggagcg aaccgaactg aggcaaagaa taaaattcac
    13621 cgccattatc cgcaggcatg aaaaggtagc cataaccttc aacattccag cgaacattca
    13681 aggtaaattt tttaattccc ttcgtattac tgatagtaac accttgattt ttaacagcaa
    13741 catcggaatg aaaggtgtca ccattcatat ccatcaaata agcgaactcg tcaagatgcg
    13801 gaccgcgccc tttttgaatg aaatatggtt taaatatcaa tgtcttattc ggcatttttc
    13861 acacaatctt gggcactact gcaaattttt ccactgttct ttgagcttca acaaccgttg
    13921 ccaagcggct tttctttcac cattcggact ccgcaacagt ccgcccgttt tgatccaggc
    13981 ataggggtca acaaaatcat accagttggc ggcttcgatc cacggtttac tatacgctaa
    14041 tgtatatact ccttctaacc agtctgcctg caattcttca tcccaatggc gatgccagac
    14101 ataaggttcg gtgggcattc ctaactgacc agtattgatg gagtctttag tcggaccgga
    14161 ggacgcccca atttcagtca actgcaccgg ccgtccgaat ttttccagtc gctcaatcag
    14221 gataattgta tcctgtaggt cacgataggg aaaatacatt tgttgaccag taattgtaaa
    14281 atctactccg gcatctacta ggtcctgcat aaattgatga ggcgtgcgct gaggatagat
    14341 ggcatccagt tcaccccatt tttttaactg aacatactca gcaaaaggac agcagttatt
    14401 aatcagtcga tgaacttttg gattagtggc tttggcaact tcacaagcca gtttggtaac
    14461 attgacaatc tcttcaggtt ttaagcgtag ttcgttcgcc cagtcatggg cttcattaac
    14521 tacttcccag gcatacatac cttcaccata atgactgacc acctctttgg tatgactttc
    14581 gacatatttc agtaattgat cgtaagattt atggcgcaac caatccggag tcgtggaatt
    14641 atagaaccag aaaagtggac gcccttctac cgtaatcccg ttttggcgta gctctttaaa
    14701 aacggcatct cgtaaattga aatttttctg cccctctata ggctcaaaat cctgataata
    14761 accgctgagc agataatggg ttatcgtggc atagttgaag acctcggtaa atcgctccat
    14821 aaatagttcc ggatccattt ggtagaaagc tcgcgaatcg catccaatga aaaaatgcgg
    14881 ccggtaacca attttgcgaa tctcaaaatc agctttttcc agttccatct tctcgccaac
    14941 cagcatagca taacccaacg ctctctgggc atagtcaccg cagatttccg gtgatgcctg
    15001 cgattttacg gcatcctcat agagactgtc ggatagcgcc agaagctctt ttacttctcg
    15061 tgacggttgc cagccatcac gtcggagtaa ttccaaacgg cgccgattgc gcaacacgcg
    15121 gcttttagct aattcaaaat tgagattcaa ctcgcgctgt ttcccttttg ggggcaattc
    15181 ataaaattcg ccaccgttat cagcagtaat gtagatgtag ccgaaacctt ccacattcca
    15241 gcgaacatca acgccgaatt tttgggtacc ctcagtatca gaaattttca ccccttcatt
    15301 ggatgcggta atgttagaat ggaaagcatc ccaatgttca tcactggcat atgcccaatc
    15361 caataaatga ggtcctcgcc ctttttggac aataatcggt ttaaaaatta attctccggc
    15421 ttccgtatta gttttcgtgc gtttgggcgt tagaacaagt ggtgccgata aagctcgtcc
    15481 accaactatt ccgcccagac cggcatacgt agccattttt aaaaactttc gtcgggttat
    15541 tcttggcata attctatcct ttattttgtt cctgatgttt ctaaccactg gcgcaatttt
    15601 ataagtcgtt cgtaagccac tttggggtga cgacctcggc cgcttagtaa accgccattg
    15661 tcataatagg cgccattatc aataaaatca aaccatagca ccgaatcaat ccaagactta
    15721 ctataaccaa ttaaatataa agcctccgcc cagtccgctt gcaattcttc gtcccagggg
    15781 cgtctccatg aataaggttc agtcggaaat cctaaactac cgttcttgac cgattcctcc
    15841 gaaggaccac tggtggcacc aatctctgaa atttgaactc tcttcttgaa agattcatag
    15901 cgctccatat taacaatgta atcttgtaaa tctcggtatt gataataata ttgcagtccg
    15961 attatgtcaa aatccacatc ggcggcgata aggtcacgca caaattgcca gggtgtgcgt
    16021 tggggataaa gagctttttg ccccgaccaa tttcccaaat gaacatattc tgcaaacgga
    16081 caacaattat tgatcattag cggtatatta ggaaccgtat cacgggctac ttcacaggca
    16141 aatttcgtca attcgacaac ttgatccgga tttaattgga cttcattagc ccaatcgtga
    16201 aattcattca ccacttccca gacagccatt cgatccccat aatgaccgag cacttcccgg
    16261 gtatgagcct cgacatactt ctttaattgg tcaaaattct tatgcgccaa ccactcaggc
    16321 gtaacccatt tatgaaacca aaacagtaaa cgcccttcta cttttatccc attctgtcgc
    16381 agaacattgc agagtatatc gcgatgggta aaattcagct gcccttcaac cggttcaaaa
    16441 atgtccattt ccggatcgcc attgacgaca tacgtcacag ccgcaaaatt aaaaagacga
    16501 gtaaaatatt ccacaaaccg atctttgggc atttcatagt atgaccgagc atcgcaacca
    16561 aaaaggaaat tcgctcgacg ccctattcgt ttaatattcc atttggcttt ttccaattcc
    16621 atcatctcgc taccccataa ggcatagtgg agagcttttt gagccagttg agcacagtcc
    16681 tcgcttaagc gcgattttcg gccggcatct tctaacaatc cttccgacaa atctaaatag
    16741 gcagatgtct cctttgaagg ctgccaccct tctttgacaa aattaacacg acgctggcgg
    16801 tttcttgcca cccgactggc ggcgatctcg taattcaaat tatagacggt ttgcttcccg
    16861 ataatcggta actcgtaaaa ttcgccacaa ttatcagcgg tataatcgtt gtacccaaaa
    16921 ccttccacat tccagcgaac attgattcca aagcgcttat gacccttggt attcgaaatt
    16981 tttattccgt cggcatcaat ggtcatattc gatggaaaag tatcgccgtt ttcgtcagtg
    17041 gcaaatgcta aatcaccgag atgaggacca acaccctttt gtacaaagaa tggtttaaaa
    17101 agcagttcgc cagaaggact gggtgcttgc gactgagaat ttataatatt tgccataacc
    17161 gctatatatc tccccgaatt attacttgat ttccatccta ttcaactact tcttaaatga
    17221 accagaaggg acttctaaca aaactccggc gcttacgggc gcgccaaaat taggcgaatc
    17281 atcgttattc caaccaaatt tctgcattcg tacgtcacga tcccagccac ttcccttata
    17341 tttagcggcg tggtaaacga tccaatcctc gcgaccatcc gacgacttga caaacgaggc
    17401 atgtcccgga ccgaatacag tttccgtttt cgaaaaaacc ggctcgggct ttttaatcca
    17461 actcgcgcgc gacaaaatat ctccccccgt aaaagttaat tgacccaaac aataatcatc
    17521 cgtccaactc ccgctagcag aataaataat gtgaatttta tcatttttct ttaatatctc
    17581 tggcccctca ttcaccaagg gacgaccgac cttttcccat tcatattcag gttcagaaat
    17641 cagcactcga tcgccactga tcgtccaggg attgctcatc ggtgcaatgt aaagattctg
    17701 ctgaacattg actttccctt cccaacccga ccaaatgaaa tagagacgat cgttatattc
    17761 taaaatggtt ccgtcaattg cccaacaatc cgatgaatcg gcaattttcc ctaccaaaac
    17821 ataatgccct tgcggatcaa tattatcact ttccagaaca tacatccggt gattttcatt
    17881 cgcgccatcg tcagccgcga cgtagatata ccatttatta tcaaagaaat gcaactccgg
    17941 cgcccataat tcttccgacc agggattacc tttcggcggc tgccaggcaa gcgttggttg
    18001 cgctaatcca atttcatgta gattcctgga acaactaatc cagatgcgat ctcccgttga
    18061 aaaacagtaa taataaaaat tttggtatct gattacccag ggatcggcac catcggtgac
    18121 aactggatta gtaaaagtga ccggtatttc tgactcctcc tggcaatact ccagacggac
    18181 aataaccgaa atcagcattg tgccaagtat aataactttg atataatgac ccaaattcac
    18241 cagaacctac tcctgattgt aaacaaattt taggtgagca ccttctgtca gacgtttatg
    18301 ggttattcca aatgagtccc atctctcacc gtcaaataat atttctttca aacgagccga
    18361 gttcaccaat tttcccgatt tttcgatcac aatctgtttc ccggaataat attccttgtc
    18421 caattcaata gttactttct gaaacaaggg aattcccagt cggtactcat ccactgccgg
    18481 acaagccgga tagaatccca gcgccgagaa aactaaccag gctgcaatcg caccagcgtc
    18541 atcattgccc ggtaaaccgt cggattcagt tccaaaatcg cttgctaaaa tttcctgaac
    18601 cagttcggct gttcgatatt cattgcccgg gatataagaa aacaaataag gataagcaat
    18661 atcgggttca ttggttatag taaagtgtcc ctcagaaaaa cactgctcca attttatttt
    18721 gaaaggttca tacccaccga aaaggtcaat gagtcctgaa acatcgtgcg gcacaaacca
    18781 ggtgtaattc caggcattgc cttccacata gcctggaccg ccggaacctt cccagtaacc
    18841 agagccttca gttgccagcg gatcaaaagg ttccaaccac tgaccatttc tcagttttgg
    18901 gcgcataaat cgggtttccg gatcgaaaag gtttcgataa aatgaggctc ttttaccgaa
    18961 ctcctctgca atttgatttt gacccagcat atttgccata ttcccgatac aaaaatcaga
    19021 tagacagtat tcaagcgtcg tcgagaccgg tccccaaacc caccaggctt gactcgtatc
    19081 ttgttcaaat gggatgtaac catattttag cagttcgtgg taaccagccc gaattggcgg
    19141 ggctgattcg ccagaatcca gtaacgtcgg tttgagcatt gcctccaaag ctaaatcagg
    19201 atcaaaatct tgtatccctt ttagataact atcggcgatt acgattggtg caggatcacc
    19261 gaccatcata taagtttcat taccagcgag ttcccacttt ggcagaaagc cagattccga
    19321 atacatatca atcatcgtcc gaataatcgc tgattgctgt tcaggataaa tcaacgttaa
    19381 aagaggatgt aaagtgcgat aagtatccca gagggaaaaa accgaataac gttctcggtc
    19441 ggtatattta ccgacaccgg tttgccccat caaaggaaat tcgccgttga catcactaat
    19501 aatattggga tgaattaaac aatggtagag cgccgtataa aatttcgtca aacccgcttc
    19561 actaccgcct tctaccttaa tttttgataa ctgttttcgc cattcgcgtt ctgcattccg
    19621 cccaattttg tcaaaattcc aatcagtaat ttcggcatct agattttgcc gggcattagc
    19681 gatgctgaca taggaaattc cagttttaat attcacaatt tgcccgttct ggcatgtaaa
    19741 agtcatccag gctccgatac tactctcatc cgaagctcgt gaaatatctt cacttacttg
    19801 atcgtctatc catgtaccga atttctgagg agtggcactt accctcgtaa caaaatatac
    19861 ctgatgctga ttatcttcac cacataaacc acctgaccga ttataaccct caatttcagt
    19921 aggcgaaaca aaacgaacgc tacctccttg tcgctccact aaactgcgcc cggcatcaat
    19981 caaaatatta acatttccct ctttaccaat ggtaaactga ctaaaactac agcgcgtcgt
    20041 agccgtcact tctactttca catccggttc attcaaattc accgcatagt agcccggttt
    20101 agcctgctca ttctggtacg aacagcggta ctgttccggc tcagttttaa cttcgcctcg
    20161 cgttacagta ataatcacac tgcccaaatc ggcacatccg gttccactaa gatgaacctg
    20221 tccaaaacca taaaaatagg gctcgccgtg aatgtagccc gatggcgctt ttaaatcagt
    20281 atgcggacta accgacacca ttccccaggg aaccagcgcc cccgggaaag tgttaccgcc
    20341 accgctcgtt ccaatgaatg gattgacaaa ttgattaaga tcgttaatcc cagaagatga
    20401 acatccattt ataaacagaa aaaaaagtaa aaataatgat atcttaaagc gcaaaagtaa
    20461 ttctctaatt ttcaaatttt gatgaataat ctctttttcc acatacccca ggcaacggca
    20521 tacatcatca acacaaaagc aattgcaaat agtaaagaag cattgaccgg actggctatc
    20581 ggtaaaaata cgttctcata aatagcatca tgaagcgttg taaacgtacc atcagctcta
    20641 ttccaggata ttgcccataa tagattagcc agcaattccg ataaagtata aactgtaatt
    20701 gcattcatac caaaaataac aaacggcttg gtgccccagg tcttaccttt cacatccaca
    20761 atccaataaa ttgtggcgaa gcagttcaag gcccaaccgt ttataaaaac tacataggag
    20821 ctagtccaca ggtttttatt aatcggtagc cagatgttca ttatgatacc gagcaataga
    20881 cataaattgc cagcgacaaa cattccggcc gttttctctt cttgactacg ttcactgcgc
    20941 aaccagcgac cggcttgaac acctaataaa gttgacgcaa tcgctggaat ggtgcttagg
    21001 atgccttccg gatcaaagcc gaataccggt gcaaaagccc aggtgtgttt gcccaataag
    21061 gttttgtcta taaaccaagc caggtttcct tcgggttcaa ggactcccgc tccgtaaccg
    21121 gggaccggaa tcactttgag agcaaaccag tatatgccca acaaaatacc aatccagagc
    21181 cactgtccct taggttttac attcagataa ataagactcg tgaaaaaata acagatgccg
    21241 atccgttgca aaactcctaa aatacgccag gtagcaatcg aaaattgcga tccgggaata
    21301 agcccaaacg ggaagccatt aatgaataat cctaaggcaa atagaatcag caagcgcttc
    21361 aaagaatgtc gtagaatctg ctgatgagtg tcaccgtgtt tcagtcggct ctcaaaggaa
    21421 aataccattg acataccaac cataaataga aaaaaaggaa agatccagtc ggtaaaagtc
    21481 catccatgcc actcggcgtg taataattga ggataaacgt aatcataaga accggcatta
    21541 ttgaccagaa tcatagccgc tatagtcgca ccgcgaaaga catccaatga ataaatacgc
    21601 ttagaatgct ccattgtccc tcttaatcac cttctcttat aaatttttca taacgtcttc
    21661 ggtactcgct tttatactgt tcaaccgtct gatcataact catattttct atgttctttt
    21721 taaaagacca ttctaaatca acttgacgag tatcaactgg gagagagacc gcattatttt
    21781 cttggttaat tgaacgtccg tctatttcaa tctttcgcac ttccataaac cagggcaaat
    21841 gaagtataat caccgctggt gcttttttaa aattattttt caatactaaa gtcgctgaat
    21901 tctttttaaa tgttaaggtg aaattaactt caccgaattc cgtgggtgca ttgttaatac
    21961 caatctttgc gtctttattt acccaattcg gcgatattgc cgatagtaaa tgcaaggtat
    22021 cactctgttc acgcaccatc atattgcgca agagaatgcg atattcagcc gcaaaccagc
    22081 catgcggtgc caggttcatc ccaaaatccc ggtccttcca tggtagaata gaaaactcaa
    22141 aaccggcctg agtggcgcta gtatgtaaaa gaatagcata taattcttta attaccgttt
    22201 cctgctcatc gcggacaagc gctgtttccg tgtttctgag ggttagataa tggtgaagcc
    22261 agcgaccacc gccataagtc ataatgccct cctggtactt gctacggacg gtttgcagtg
    22321 tctgggtaac ccacgggtga ttcggtgata gaatcacttc tggatagaca cttagcagat
    22381 taccccaatc ttcgccgcct tctccatcca atccgggagg aatataacct ccggtctggg
    22441 cggtaatttc tgccaggatt cgcattaaag tcgcataata atcgtcatat aacttttgta
    22501 actgttgaga tttttcccct tctcccaaat aatcagctat aaaaacggcg ttttttattc
    22561 caattaatgt ccagaagtta tggccggtaa cgtgtccggt aatattttcg ttatcaccag
    22621 gagtcgttac tggaatcaag ttcaagggat cagtttttcg ggcgctttcg agccaggcaa
    22681 tggcttttaa tacagaagga aacaccttct cggcaaacgc tcgatcatcg gtcatccgga
    22741 catgctgacc ataagcccaa agtgtctggc caagtccatc atactgtcca ccttgcgaaa
    22801 caaaattacc atcctctcgt tgccaacgcg ggaaaaaatc caatacccgt tcagcttcat
    22861 cactataacc agtaagatca tacatccggg taaaatacgc cgcatcccgt aaccagaaat
    22921 tatcatattg aaattcatta actttttgaa cataccagcc gtcaatttta tcacgtgcaa
    22981 ttaagctatt gattaggttg gcattaaaag tgtcggttac ctttttctcc ggtaagacaa
    23041 ttgtcattcc ctcttgcaga atattttccc agaattgaat cgtcttatta agataatgat
    23101 caaattgtgc ctgccgaatc cattttgaat actcatccac cgtatcaacc ggcatatagg
    23161 gaaatttgaa atcaagaacg acttcctcgt ttgggagcat cgtcaaagga tactgtacaa
    23221 tccccaccgg tgttgttggg agaacataaa gggtcatctc gccttctgcc ggtggttcat
    23281 tatagcccat tttccaggtc aaaaatttgc gaattttctc acgtggcgga aatgagtaga
    23341 gtaatttatc attatataaa accgcatcgt cttgatgtaa acaaagccag tcctgacgga
    23401 attccagccc cgcttgttca tattcaccga tttgttccgg taccgccggt cttataaaac
    23461 gattatcacc aatcccccaa tcggtattcg cctcattctg gtaacgaatc ccagccgcaa
    23521 accaggtagt cctttgctga gaatttcgat taataatttt tacccgaaca aaattgacca
    23581 gcggactttc tggcttacca tccaaggttg cagcaaaaat tgtccatcgg taatcgaaac
    23641 cgtcacgatt tagtgtatat tgaatgaccg gtagataacc atttaacaaa gttttgaccc
    23701 gtttttcagt cggtattggc ggattgccgg tgaaaaacat catttcaccc gaaccggtat
    23761 ataaatatcc ttcaggagta atcaacgtcc cctcgcgacc atccataact ccgatctgat
    23821 cagtcggttt actaaaataa caaaaaggtt cgtcatcccg atcaatcgat gggtttaaca
    23881 tctgggcatt caactcattt ccaaaaagta ttataatcaa ccagagatat aaccaatttt
    23941 tcatgcttta tcctattgcg gtagtgccca ttctaccagc tttacgttca ttacgatttc
    24001 atcgagtact ttactatcct gaattatttg ccacttcaac ggctgaaatc cgtccgtctc
    24061 attatctgga gcaaaataga aacattgtac cggtaaactt tctccggcag gaatgatata
    24121 ctcaccgctt ccactgactt cctgccaccc attcggtaaa atcggtttga cgctcagtct
    24181 tatcgttttt ttatcaccgt tcgttatcag gagtggcacg tgaaaataac tccccaccga
    24241 aatggcaatt tccggcgaga ctattgtcgc caagtgctga agatcatgca cctgccagaa
    24301 atggcggtag taatcgaaaa taccaccgaa ttgtagttta acacctctcg ctactggcga
    24361 aatatagcca gtgtttttat gaaaatctgc tggtttttcg ctgacacctt caaaaacatc
    24421 accggttggc tcacatttta caacggattt accgtatatc agatggaatt tttccagcca
    24481 ctcgatcatt ggttgccaat ttccagattg ctccgcttgt tcggcaattt gagctacatc
    24541 cgcctgggtt tggtggaatt tatgcagttg cgcggctaat tcgatataag gttttccctg
    24601 cgaaggtgat ttggcactaa tatcaaattg tggtccggga gcatttaacg cttcatcacg
    24661 atcggaaaaa taataaatct tcttcacctg ccagggccgt aatccctcgt aaaaattatt
    24721 aatatccatc gtttcccgcg gaaaagccac ctgcgccgga aaaattgtcg gatcgcctgc
    24781 catatcgaat gcttccgtcg caataactcc agccgcctga tgatcaccat gattttcccc
    24841 cgctacaaaa tgaggcaacc aggttacaat gatttccggt cgagttaatc gtacatagcg
    24901 aaccaatttc tccaaagccg ctccatgagg aagattctgc aaggaatgca aaacatcttg
    24961 cccgggtgtg tctatgccat cgagaaacca aacattataa atgccaaagg cacttaatgc
    25021 gcgccgcgct tctatttccc gaatagcccc cattgagtga tattgctctt taccaatcga
    25081 attaccgcca ccctggccgc gattagtata aatcacccca atttttttac cgtcgtcaaa
    25141 aatccatttt gccagctgac caccaattgc agtttcatca tcgggatggg caacgactaa
    25201 aagaatatct actttggaac gtggatcggg tgtaattgcc tgagcttgga gaattccgat
    25261 tcccctgatt gtcataacca caaaataaac tatcagcgat ttttttatca tttgtcctgt
    25321 cataatatta ctctgttcat tatctttcca ccgattcaac cacaaaaata ttttggcggc
    25381 gtggttctgc gacataaaat acccaccaaa ttaacgaaag aaatcctccc aaaccagcaa
    25441 ttgcaaagat tacatcataa ccatatagat ccaccaaata acctcccaac aaacccaata
    25501 aaataaacgg actggtaatc atattagaaa ttgatatata agtcgggcgc tccttttcgt
    25561 tgcatatctc tgccagaatt ggcaaacgag aaacttgaat catactcgta tttaaagcag
    25621 ataacacgaa cacaaaataa tataaagaca aggagcgcag tgttattgcc agaacgcagc
    25681 tggctaaaac tgaaaatgcc gctattatca tattgactcg gtgaccaaac cgatcaccga
    25741 tggaaccgga gcagagattc aagatcacca tactaagcat atatatcatt gtaaaactgc
    25801 cacttgatgc ggaatctaag ccgaaacgtt gcatcgcatt cacgataaaa aacggctgag
    25861 caattagtcc aatagtcatc agactatcag caatcaagaa attcagaaag ttatgattac
    25921 tttttatcaa gttgggcaat tgtctgaaat attgaaaatg attcaatgac tcagtgctgg
    25981 cgttcgcctc ctgttccttt agaaaaatta aggaaacgta agacacggtc atcattacaa
    26041 acgctaatag aaataaatag ccaaaattag cgggaaacgg ttgggcgcta agaataattt
    26101 taaccaacca gccgcccgct attcctaaaa ttgcaccagt gatagttcgc aaggcgaata
    26161 accgcccccg caaattaacc ggagtaatct ttgaaattaa atcaaaccaa ccggggtagt
    26221 ttactgcgcc tgcaattgcc gccaatccaa acgcaataaa aaaaaagact aatcccatgt
    26281 tggacgagac attttttata ataaaaaaac agagcaatgc cattaataac cagggtaatc
    26341 gttgcagagc cgcagttttt aagaaaaatg gctttttata aggtaattgg cgggtataat
    26401 tggcaataaa aatctgagga aaattaactc cgataatcca gatcacctga accaatgcca
    26461 cggtaatatt attaccgcct acctgtcgga cgaacaccgg tagtacggtc ataaccggaa
    26521 caaagctcat tccgaaggcg aaaaaggttc catccatcca attcaatagg aaatttcgac
    26581 ggacttccgg atttataagc aatggttctt taaaatattt cgccaaagta aacctttact
    26641 tctttcggat ttaaggtaat attaaatgat gagtcgttgt cttgatatac gacatttaag
    26701 gtttcggctg aaaattgttc ggctaagtgt tttggtatca atccacatat atgaatttcg
    26761 gtattaattg ccgtttccgt gagattcctt aaaatcagca tcatttttcc cgattcattt
    26821 ttcagcaaat caacccgaat ctgtccaagg gaatcactgt aattagtaga tatattagca
    26881 tattttttaa tcagtcccgc caaaaaatct ccattggctt gaattccctt attataaaaa
    26941 ggcaaggcaa tataagaccc gatcaatact gctttaccaa gtcccaattc agccaaggta
    27001 accgccggtg atccgtcagg aaaataccta attaccttag ccccctcaga gatcaggcgc
    27061 tcttgtacga tcgccccctt gactacaatc tcttcttcat tatctaatac atctgaatct
    27121 actaagatat cgaccaaatc attggtatta actggatcaa ctactccctg acgagctttg
    27181 aagattttgg acagtccgta tccgggtacg attttttcat gatgtccatt ctctcgattc
    27241 caagcggcaa aataactttc cccgatcaac agtccacctt ttttcaccca atcttcgagc
    27301 aaatgacaaa tcctctggtc tattaaataa ggaaacggaa aaatgagcac tttgtaacga
    27361 cttaaaagct tttcatcaat ctcaatcgga tggacgaaat caatgttata attatgttca
    27421 tataaagcct tatgggcccc cagcaaagaa ttggtagcat tcttttcatt accagtagcc
    27481 gcccagataa aaatttggtt ttcaggatta taactaatcg ctatctcagc cgattgtggc
    27541 tgtgcctcca gtaagaaatc agcattcttt tgtaatactt gaccgactcg ggcaaagtcc
    27601 tgtaaccatg aagttttctc tccatccaga gttgttaagc cccaggttgg tgcttctcgt
    27661 ccaagaatct caggacgata ttgccagaaa ataaaacctt tcaagttccc ggcgatccca
    27721 ctgaagataa aacgcttcac atcattaata tcaatagacc tcggtaaatc aagcgtatag
    27781 ccgtttagca ttaacatctc agccgagatc accggtttgc ctttggcaca actacgtaaa
    27841 atatcaatca tcgccggatc atccatctgg gtaaatccgt gtaaatcgcc gaagcgtccc
    27901 acattccagg gatcactcgc cgtagaggtt acgggaaagc cctgaataaa aacatgatga
    27961 cacatcaatg ggtgcctacc ctgatcaata gctttggcaa cttcaaaacg tttcctgaca
    28021 ttttcgccca gtgtagccac aaaaaacatt cgccaatcaa tgatatcgtt gaaagtgttg
    28081 cgtgttaacg gtaattctat ttcttcaaaa ctagtataat ttcgattcca ggaaatattt
    28141 aaccgttcga tcgtttgata ttttagttct aaccagtttc taaactttac ccgacaatta
    28201 tcacagtagc agagcatatc gcccatttta ctcgcatcat ccgcatattc acgcatttcc
    28261 gccatactac tagtcaattc cggttcactg cctacattcc aaattgctaa tgccgggtga
    28321 ttcgtgtagc gtttgactgt ttctgcgaga aattggaaaa agtgctgcat tacgccatca
    28381 tgattgaaac agtaacctaa tccaccaatc tgccgatggg gttgagtttg gggaccaatt
    28441 ttccgtccgg acaacgttac catagaagcg tcgggaaatt tgtgataaat ccaagctggc
    28501 gccacgtcaa aaatcgtgtt cagcataacc tttagcccaa agcgcgcgga caaatccatc
    28561 aattcgtcta tatcatcgaa acaaaaccga tcttcgctcg gctgattcca gcgccattga
    28621 acccagaatt taaccgtatt gaaaccatta tctgccatat tcttaaggtc attttcccat
    28681 tcattacgag atggcgatgg cgcccgataa tactgagagc cgaagggtat ataagattct
    28741 tttaaaatat tgcgcatcac aaatgctctt tattaattgt gctaaaatga attaaataac
    28801 atactgtttc agatacttta gacctaattt taatcccgtt tcgacttttt ccacagaacc
    28861 ttcccagacc gggtcttcgt gttcaatcga aagcacataa tcatagccaa attcctgaag
    28921 cgtccgaatg aaaagccccc aatcaatttt acccgagccg ggcatccgat aacgccacca
    28981 accggatttc cagggaattg gatcaatttg ccggctaaag attccgtatt tgtattgacc
    29041 ttccggtaaa atttcggcat cctttgcgtg cgtgtggaaa atccggggcg caaaatcttt
    29101 aatcacccgg atataatcaa ttcccagcca ataaagatgc gatgggtcga ggttcaagcc
    29161 aaaattctca gaggggactt cattgaacaa cgcttccaga agttctggtg aataagcgta
    29221 attcccgggc aacccaaatt tcagccaatt ctccatcggg cagttttcaa tcatcaattt
    29281 gacattgcga tcttcagcat attttactaa ttcacggaaa acgcaaccaa tttcctgaat
    29341 gttctccttc ggtgtcttat caggacgagc gccaacaaaa gttcctacaa gctcaacgcc
    29401 cagcaaagat gcggtattta tgactttttt caggtgattg tgataatatt cccgttgttt
    29461 taaatcggga tgcagattat tatcgtaata ggcaaaagct gaaatttcta tctgatattt
    29521 gttaaataac tcttttactc gctcggcatc ttgtttggag aaattagccg catcaatttg
    29581 acgcgcctga taatcccgat cagaagccac tggccaagcc gccaattcca acgcttggaa
    29641 gccctgttca gccgtccact taacgatctc ttccaatccc agttgtggca aacaagccgt
    29701 taaaaaacca agtttcatct tttatctcct cattgttgca ttaactagtt tataccttta
    29761 tccaacgttt ctcttggtga cttttgagaa ctgcttcgac tatgcgattt tcttgcaatc
    29821 cagcttcaaa tgtagggaaa ttttgctcgt ggattgccaa cttaccagtt cgtaatgcgg
    29881 cgtaaaaatt caaaaacaaa tttttgaaag tatcaggata accttcggga tgaccgccgg
    29941 gataatgagc gaacgaacga ctatccgctt ctaacagcgc cgggtctttt actaatatct
    30001 ggttggcttt atcgcggtaa cctaaccaga gttgattagg ttcttcttgc ttccaagcaa
    30061 gagacatttg actcccatca atagcgatat caaagccatt tttgtaacca gcactcactt
    30121 gagaaacatt gaaaacacca tgcccacctt tttccagatg gaacaacact gcaccagcat
    30181 cctccgtctt aaccgtaatg catttagtcg aatccagggt cacttcctta cccttgaaag
    30241 ttgctacatc ggattgaggt ttcctgcgcg atttatgaat gattaccaga tcggcaaaca
    30301 cttcaatgat tctctgacca gtaatgaatt gaaccaaatc acaccaatgc gagccaatgt
    30361 cggctatcgc gcgggaattg ccacccattt ccggttcaac gcgccaatta taatcttcag
    30421 gataaattaa ccaatcttgc aggtaatgcc cgtgtgccag aaaaatttct cctaattttc
    30481 cgctaacaat taattggcga gcatgctgaa tgatgggata attgcgataa ttaaaattta
    30541 ccgcggtaag gatctttttt tcacgagcta atttgaccag ttcttcagac tcagctacac
    30601 tattggtcaa tggtttttca ctgataatgg ctttgcccgc taaaatcgcg gctttgttta
    30661 tttcaaaatg caggaaatta ggtgtacaat tatgaaccac atcaatttct ggattattta
    30721 ccagttctcg ccagttttca tatacctgcg agatgccaaa cttatcagct aatattttag
    30781 cattttcagg cttaatatcg gctattgctt ttacttcaat cccgggtatt cgacgtagat
    30841 tttccagatg tgccgtaccc ataaaaccgg cgccaataat gccaacttta atggtcttca
    30901 ttgttttcca ccttcattta ctcttttggc tcgatcaatc atttgatagg tctctggtgt
    30961 ctggatagct tgatcatttt catacaagca tagcgctggt ataacgttta attgagaata
    31021 tgcagattcc tgaagtttaa atgtcggatt agatggatgg cgagttaaga gatgcgccac
    31081 atatttggaa taatttccaa ataaataagt tgaaaccggg tgcactttcc gccacggttc
    31141 ttgaccttct ataccgtgga cacccgaaag gctatcaatt ccatgaatct gagctaccgg
    31201 caaacaaccg gcgacttgtt cgtgcaatcc ttcaccggca aaaagaattt caggaaacgc
    31261 tttctgtaac ctttcaatat gggttcgcat tcccttaata aaatccggtc cacgctttac
    31321 attaaaggct agtagggttt gatccagaaa cacggcatca acttgaaact tattaatcag
    31381 acgtcctaaa gtttcaatca tcaggtcact ccaagctcgt gctcccggat ttatatacgc
    31441 aaaataaggt tccgtaagcc aatcgccatc tatatccatc gcccaacctt gcaaacgacc
    31501 aaatgcatca actacttgat attttttaaa ttccgggtat ttcgggtggg tataggtcat
    31561 tcctagtcca ttggtgtgaa tcatcacttt aaaaccaagt aaatgagctg tatccaccag
    31621 ttctttaaat ttttcctcac cacctaaaag atcactggga ttataatcag gaatgtggga
    31681 atcaatgcca tgctcggcaa atcccggcaa atagagtaat gtccgccgtg gatcgtgaag
    31741 gtttttaaat tcgttcaacc gctcagtcat ctgttcaaaa gtatgatgcg gtttttgaga
    31801 ttcacgacgc gccccccaaa tttccaaaat aaaattgata ttctttgccc aattgggaaa
    31861 attttgtttt tgaggaagtg gttttatctt gaaggctttc tccatccaat tacggtattc
    31921 agccaccggt ttctgccagt cgcctgtgaa ttcctcgata aaccattccg tttcaaagga
    31981 agttagcctc tttttggcag gcggttcaaa tccataacta atcgtaaaac tatcattttc
    32041 acgccgaacc ctagctagtt tcagattgga agaagaatca tgtgaataaa ttataagtcc
    32101 tcgatttccc tttattccga tgacaaactg agcattcagc cagaaaggat atttatagga
    32161 caatgtcgtt tgttcaggca tttgatccgt tattacctga ccgcccagtg ccggtataat
    32221 gacttcagcc aaattcaaat tcgtaagact ccactctacc aaccagatcg gttccggagc
    32281 cgaatacttc gcttgaaatc gcaaaccgga ttgagtaaaa gtgcagtgca cctgacaggt
    32341 aaattgaccg gaattatcct gtagaacaat gtcataacca tcgactgacg atttatattc
    32401 gattttttgg gtcaatagag ctggctcgga aacggctcga taggttcgaa tcaaaaatag
    32461 ctggttacta tcatcaccaa ttcgacttga tctactattt tggcaagaat tgctcatata
    32521 agtatctcga tgtcatttat tgattttttt tcaatatctt tctgggaagt ctgatagcga
    32581 aaaatcacga aataagtcgc ttcggatttt tctaaaggaa tgcatgcacc atgccaaacg
    32641 ccgggattaa tcacgaccgc ttcaccgaca tttaccctaa atattttcaa attttcctca
    32701 ggttctcctt caattaatag cggtaaagca aaaggtgcgt ctaccggtat caaaatttca
    32761 ggggtatgta aatgacactc taaactattt acccgattac ctcgaggaag agtgaccgtg
    32821 cagagtccga tttcggtttt accattgatt cgataattgg caatattcgc ccagaattta
    32881 aaggtatcac cctcagcagt tggtttttcc ctaggaagcg ctacgacctt gccgaatttg
    32941 gcaaaattaa taggattaat aggtataatt ttcttaaccg gaatgttcat tgctttattt
    33001 ttccagctta aatattttga tctcaccgcc tttaagcacg atgttattta gtttggtttg
    33061 gtcgtcattc cgctgaaatt cattaaaggt ggaggcactc ccaagttcat tgtattggac
    33121 gacactccct tttaatttgg ccggaatcgt aacggaaatc gtttccgtgc gtgtatcttg
    33181 aaccagattg gctggcttta agggacgctt gctcggtacg ttagtagcaa tccaggcaac
    33241 gaatataatg gtaccatctt ccttttcgaa cgcacgataa aacacatccg aatcaggctg
    33301 atgctcgacg gaaatcaggc tggtaacgtt gcggtaagga ttcccaaaaa gcttgcggaa
    33361 aaataccagg gctttctcag caggtttggg tctataatcg gcatacgcaa taccaaggta
    33421 acgattgtta taattatcac cgataacttc ttcgccagcc ggcaaatctt taatttcata
    33481 ccaggcgatt gccgataatt tttcctctgc caccgctaaa gtaagacatt taaataaatc
    33541 tacggcttga tactccggtg tatgttcata atcataataa gcataatagg aatcggatac
    33601 gtaactacct tgcctaaatg tgctatagcc aacctctgcc atccagagtg attgatcatc
    33661 tccatatttt tcaattactt ctgccatcgc attaatattt tcagttattt tttctacggg
    33721 gcgttcactc caggtttcat aataattgtg catattgaca atatctatat aagggctgag
    33781 accataatca cgaaaaagct gctccagaaa atcggcgtga taagccattc cacctaaaac
    33841 tactttggct tccggatctg ctcgtcggac agcctgcgcc cctattttaa taaattcagc
    33901 gaaagcggcg acgtctcttg tatcccaata aatatccaaa tccggttcat tccagagttc
    33961 ccaggtttta atgtattttt tatagcgagt tacaagatca tacataaatt caccaaattc
    34021 ttcgaaatcc agcggctggt cattccagaa ccagagagta tcgcctcttg aattccagag
    34081 cggagtatag cagatatagg gaattaaagt tataccgtat tgattcacgg cagttttaac
    34141 ataatcatcc cagaataacc agtcgtattg atctttctgc gcctcaatag catcccagcc
    34201 aaaagaaatc cttaaaagat caaccccagt tctcttcaat aactccatat cattatgaat
    34261 aatttccatc gtcgtggtct cttccgggta atcttcacac aaaccaactg gaatgtgaac
    34321 aaattcagcg tcttttgatt ttagcagacg ttgatagttg actgaattgg gtctgctaca
    34381 atcaacaata ataagcataa ttagcgctaa gaatgattga agccagtaat attttttcat
    34441 attttttacc tttcttaaaa cgttaggttt agtcaattca cactctatta ataccttaaa
    34501 atattataat ccctactttt tagtccattc atttattaaa aatcaacccc tgatatcaga
    34561 attatattgg cataaggcga tgtttcaccg ctacgaacaa aaacagcact gtccgattcg
    34621 cgataaatat ttttaaattc ttcatgggat acatactcta ttggaacacc ttttaataag
    34681 tctttcactt cttttaaaat gccattatta attgattcga tttccttagc caggattgcc
    34741 ttttccacaa tcaattcctc aagtactgct tttaaagtat caatgaagcg aggtacgtta
    34801 ggaactaatg ctaaatctac aatttcggcc tcgcgtggta agggaagtcc tgcatcacaa
    34861 ataactaatt gatccgtgtg tcccaatgat gcgattacca ttgagagatg agagttcaag
    34921 attccatgtt tcttcatcgc ttttacgtcc tttctattga tttaaataat ttgaaatatc
    34981 agcgagggtt ggcattgagg tctgagcacc ttgcttcgtc accgaccagg cggcaacttt
    35041 attggcgaaa ggtatcgctt catttaccgg ttttccggat gccagcgccg cggcaaatgc
    35101 cccattaaaa gcatcaccag cggctgttgt atcaacagct ttcaccggtg tcgcaggaaa
    35161 ttcttgccat ctatcagaat taacgagaat ggcaccgttt tcgcctctcg tggtaataac
    35221 attgcaaact ccagtattaa ttaacttccg agaggatgaa ataacacttg cgcgatccgt
    35281 aactgactga ccagttaaaa atgatagttc aatctcattg gggatcagaa aatcaatacc
    35341 attcaacaga ctggcaggca acttacaagc cggcgctgga ttaagaataa acatcgcacc
    35401 atttttcttg gctaatatag ccgccgcttc aacagcactc agaggaattt ccagctgaca
    35461 taacacaatt tttgcccttt caaatagctt ttcctttgat cgtatgtctc tttccgtcaa
    35521 ccgcatatta cttcccgaaa cgacgactat ttcattttgt ccgtcggcat caacgtatat
    35581 taatgccaca ccggttggcg tttctgagtc tataaatatc tcctccatat gaacgccatt
    35641 ggattccata ttatttacca aacgatcgcg aaaaatatca tctcccattt taccaatgaa
    35701 aatcgtatct gctccaagtt tggcacaggc gacagcctgg tttgccccct taccacccgg
    35761 ataaattcca aacttttttc cgaaaacggt ctcgccaggt tgaggatatc tatcaacttg
    35821 ggcaaccaga tctatattgg cactacctac aataataacc gaattttttg tcattaatat
    35881 ctctttaata tattttaatt ttaagtaatt gataatttgt aattactggc ggctttaaaa
    35941 gtcctgatga tttaggaact gcttccagag tattgattag cgtattggta atagaaaatc
    36001 ggaaggtatt ttttcccggt ctgactaagt ccgtaacatc aatttgatac ggatgccagg
    36061 gaactattat cggtccgcta tcattaatct gtaactctaa gacatcttct ccacactcaa
    36121 tatcaagaaa cagctttcct ttacaaaagt ctgccggaat cgtcagctct gttgagtagg
    36181 ttccagtgcc cgagaaatag gggtatccct gttgacacca gtcaccaata tttacctcct
    36241 ttttcggcaa aacaatacta tacccatcgt cattcaattt caaagcaaaa tcacctgtga
    36301 tttttataag gtctaaaata ccgtcagttc tacgttgtac aactaattga accgcgatta
    36361 gattacgacc cgggtggaca taagaatgga tattaatttc ccgaatttca gcatctaatt
    36421 gactgcgttg gccagtatcc ttaacttccg tgccattaat aaataatcgg aaagaacttc
    36481 cactaaaacc atctataaga agttttacac gattaggtaa ttcctcaatc tgaaacggaa
    36541 ttcgatacca gagtgtttgc ggatagtgga tatcatccgg ttcttcgggc aattgcattt
    36601 cccaggcgcc gatggtaact ggtagccagt gatcatcatc ataatcgagt gcggcgtagt
    36661 tatcatttga gcattcattt gaaaccagca ttttccattt agcaagacat aatgtattat
    36721 cattttctat tttaaaattc ataacctccg gtaatattag aggcggtgga ttcttcggca
    36781 ccaattggac aatatccttt tgtttaccac ctgtttttaa agtcaatttg gcaagatctc
    36841 cattttttat atagccggtt ataaaattgg cttcagtttt ttctattacc aggttggttg
    36901 cactaatata agaatcaggt aagtcattat gtaaaataac cagaaatgtt tggctggcgc
    36961 cgaatgttaa gcgaaaacaa agtcggttat tgattatatc aaaaaccgag agttccttta
    37021 ctgcaccggt cgccggatcc catagttcag ggcgtccaac cgtctccaat gaaatgtcca
    37081 cctcgccaat atcttgctga agagtattaa caaaatagaa cagatgtcgg ccatctttta
    37141 ccctatgcag ataaaaaact tcagaatgtc cgatctggac atcggcggta atacacgact
    37201 caatggcttc tttaatatcc tgtctgcgcc gatcttttgc cagcccgcca ccttgtatta
    37261 agcacacccc cggctggagc attttcagct ggttaacctc gtttttaccg ctttggaaat
    37321 cggccaaaat acaatttggc ttgacgccaa atagttcatc tacagcgcga gattttgacg
    37381 gtttgccatc aataaattgt tcaaccggta ataaaatatc ggcaattaac ttaccaccgt
    37441 tatttacgaa agcttttatt ttatccagag ttgctttttt aatatgagtc accgggggta
    37501 aaatgatcag ttcgtaagat tcatcggcga tctcaagtcg gttaccattt aactgagcat
    37561 taattagaat gtcctcatca acataatcaa aatcataatg aagatgtaac agggcatccg
    37621 tcagataatt aaaatccatt tcaatcaatc gggaaatgtc gtcgggagcc tgcggtacat
    37681 agtttgccca aatactcgta attgggtaga gaatgagaat ttttgcaata tgttttccac
    37741 cggttagagc atgtcctaac cgcgccatat agcgagtaaa ttcattgtaa tacttccacc
    37801 aggtatggtg ataaaattgc gacggtggcc agtcacgctt gcgttcacct tcaatggaat
    37861 aatggtagcc atgattatta aatagattaa ctccgagtga atattcccag ttggcaatcc
    37921 acttcatccg ttccaaagtg caatcccaat aagtacctcc cattgattcg caaagtaatc
    37981 tctttttccc taaatggtga gttatcgagc ttgccatctt aagttctaca tgttcgccgg
    38041 gctgttcttc cgaaccaatt ttcggatata gatgatccac tccggttaaa tgtaaattac
    38101 tcaaatgttg catcacattg ccttcacagc gggcatgacc tcttaaccaa ttttcaaata
    38161 gaatgtggcc tgtgaataaa acgccgtttt cagcacacca atctcggagc tgtttataat
    38221 aactatcgtc atactgatct gaaagcgttt tccagaaatc ataacgaatt cgggctgtat
    38281 cgcgaccgac attagcatat aaagccgcta ggtgtggttt tatactatat ccattacgct
    38341 cacgaaatat cttgaacatc cgctggctcc agggaataac gtaattatca gttcctactt
    38401 gaaaatagtg catagccggt tcatcggtat aaaatcccgg cacgattttc ccaaattctg
    38461 cccccactgc ggtcttatat tgttcatgcg ttaattcaat aaaacgttta gtggcttccg
    38521 gatttaaagc atctatatag taatctattt ccttttctaa aaaatacatt aatacccagt
    38581 taccggctgg cgcttcccac ggaatgactt tattaaatga taaattcgga gttacatcaa
    38641 taatattttt gatgccagtt cgatattcct cagctgaaca gatataagcg gcaatcggtt
    38701 ttgaatcgcc ggtatttata tagcgtgaat cggtggcttc aagatagatg aaaagtggac
    38761 cattgataaa cagtgttacc atttccaggt acttttggct taaatatgga tattttttaa
    38821 taacctggcg ttcggctgtg ccgcttggcc agttcatctc atcgtaaatc cagatgttca
    38881 agccgatttc tcgacctttt ttaacggcaa attttacccg ctcaaaccat ttttcggata
    38941 aatagggaat ttttaaccca aagcgtccgt gaataaataa acccgtaata cctttggctt
    39001 tgtattcttt taactgccac tccatctccg cctcgtccat ttcgccgttc caaaaccaga
    39061 atggcaaaat acccatttcc agcgggggat tcttaaaatt ttcacagttt aattctttgt
    39121 ctggccacag ttcatttagc aacttcatca agtaattcct taacctttgt taattttcaa
    39181 tttgcaatta ttagtttttc aatttgccaa aatatcaatc agttatttta acaattggaa
    39241 aatcttagtc gccctcggga ttggctattt tttttatcat agacaagtaa aatacggtaa
    39301 aaaagggata attcatcttc attaaaacca attcatcata acctaaattg agattattaa
    39361 ctcccatgtc aatattatta aaaccttaac atcactgtaa actgatgggc tgaattaagg
    39421 taatcataat ccacaaaaga ataatccacg acaagattgg taaaatccat cggaatattt
    39481 aatgccgccc caacactaaa atgcccctct tcacgattgg tacggtaacc agtacgcaag
    39541 gcgatgcgat tccataacca gtattcggct ccaatattta accgttcggc ataatcatta
    39601 gagtgaatcg cgtcaatgtt cagagaaaga cgctgtttat taggatttcc accaagaaac
    39661 aaatctttgc cacctaataa atctattgat gtcccaatcc gaaatattaa ggggacctca
    39721 tactgttgtt cttctctttg ctgggttttc aaccaacggg cttgcgaagc gaaattttgc
    39781 gccgtcatcg caagccgaat gcccttccac cccgtattat aatacgtacc aacatcaaaa
    39841 gcccataaac tggttgaata ggtataaagg tcttctctta aatatttggt gcgaatacct
    39901 atagacaatc tatcggtaaa cttttttgaa aatgaaagac caaccgagag atcaccgcca
    39961 gagaaattta atccggtgcg agtatcaagt ttaccatcgg gactggtaac cagggcttcc
    40021 ggaatatcac cgtaatccat cgcgatataa tcaagcgcaa tacttcccaa tcggtcaacc
    40081 gttagcccaa ctgctccagc ggtaacattt agatccccaa tccactttgt atagctgaaa
    40141 gaagattcta atcgttgcat ctgagtaatg gcggcgggat tccagaaaac agcatcaatg
    40201 ctatttaaat ttgcgataca ggcttccccc attgcagatg agcgggctcc tataccgatt
    40261 tttaaaaact gaagggaggt gcgaccggct ttattaaaac cttctaccct cagatttgta
    40321 gcgaaaaaaa tcattgcaga aagagttaca attttaattt ttctattatg taacatcatt
    40381 ttttattcct cggcatatca attttactct ttagcttcat tcagcgaata atcattagct
    40441 taccaatcgt tcgtttccct tttgtccctt cggtgtgcga ttcaatggca taaatataaa
    40501 gacccggtgc tactcttaat ccggaatcgg tacgttgatc ccaggctaca ctgccaaata
    40561 aattaccata agtatctggc ccatgttcaa tcgttttgac taaatcacca gaaacggtga
    40621 aaattttaat cgtcgctctt tccggcagat tacgaaattc aattttataa ggcgtcggat
    40681 caccaaaact acgctcaggg tcaccttcca catagggatt cggggcaacg aatacatccc
    40741 agccagcagt aatgtcaact ggtccaggtc tggctcccgt caaaggagtc atattgtaat
    40801 catcactgac cagctcccct agattatcaa cgatagtatg attggccgaa acccaggttt
    40861 taggatttga ataataagcc tgaacatagt accggaaatc aaaaccaaac tgcaaatcct
    40921 tatcgagata cacatattta ccaatttctg tattccaata gcgattggct tcttcaatgg
    40981 gaatgtcaac aacttcatcc caaatgcccc gtttgtcaat accgcgaaat atccgatagc
    41041 cactcagatc agttgaacca tcataaaatt tattcccagc cccgtccgtt attaccgcct
    41101 cttcagctag tttatcccat tgaatcgcaa taatggcagt atctgaacct tgagaagcat
    41161 tagttgcgct acaattaggg gctggtggtg attcgggcac atccgccgct aaattaattc
    41221 cttcaaccgt tgctccaaat ccccacttta atgctctacg agcggcttcg gcattacggc
    41281 gaatatcggc aatcgaacta tcgggatacc agttattggg atccccacaa attacttcat
    41341 gataatccaa actcccggct acttcagcga gtattaaccg cacagaatca cctggcgcga
    41401 gataatatgg gccaaaagta gcagtataaa aacaatacat atcaccatta ttttcttcag
    41461 gaatccgatt atgagtgata aaatcgaaca ttcctttttc tcccagccgt tcaatcgaaa
    41521 agtactcgtt catatccaac gctcggcctt ggaattgctc accctcatcg gaataaatat
    41581 tgaaataatt aggatccgcc tcaaaaggat ccgcgtcaga atccgtcgga ggagttctat
    41641 acagaaacac aaatccaaag caggcgggat cttgcaattc tatattttca taactttgat
    41701 tatacttcaa tcgtgtttgc caatccgatc ttttactata ggtatcgttt ggatgcggct
    41761 ctcgcccccc attaaaatcc cgactgtaaa aaaacagtcc cttgctatca tccccatcat
    41821 tctcgaccgc aaaataatcg gtatatgttc caccattttc aagttgcttc cagcctccaa
    41881 atccaccagc cggagctgcc gaactcataa aatcaggatc gtaatgaaaa ttcagatgac
    41941 ctttagttga gacctggatt ccgctatgaa aaaccacaaa taccgaatca aaagtttgtt
    42001 cgtaatgttt gacttctccc agagctggaa taaccatctc accagtattg ataaatacat
    42061 aatcataaac aataaaatca tcataatccg gaaaactcca agccatagag cgcatcttaa
    42121 ccgaaatgcc ggttgctgtt gggaattcgc agtaattgat ttcctccggc aataatggat
    42181 taaactggga cgtccctacg aaattattcg tttttataaa gggtggataa ttccataaaa
    42241 taccatgact tccaccagca aaggagctgt gataaaaaag atattcctga tattgtggat
    42301 cataatccgg agctactaat acatcaccgc ctttaattac taatgccgga ccactacgaa
    42361 aattgtgaaa attggcatca gtaatccagc cgttagcgag ttgttcatta ttgggaaatg
    42421 aatatccaga aaagccagga taaaatccca aaccggaagt ctcttcataa tcccagacgc
    42481 ccaaatctcc gattaaacct acattattga gcgtttccca tagtttcccg cgagtatggg
    42541 ttgacttccg ctgagcaaaa gattcactcc aaccagtcaa cagtcctaaa atgataacta
    42601 agtgaatata ataatttttt actttcatag taccaaccac ttctattatt gaaaacttaa
    42661 agccatgccc acggtgattt tcctgggaag gtttgtatat ttggcataat ctggaaaagg
    42721 ggcaccgttg gtcatttttt ccggatagac cattaagtta gaattgatat aacgaacttg
    42781 atcttcggcc gaaatatcac cttccataag atcaacatta tgccatttat cattaaaagc
    42841 attctgcatc tcaatgaaaa ttgcaggcct ggtacccttg aatcctttga attcttttct
    42901 cacccgaata ttagcattct tatttgccgg acgaatgatg ttaaccgctc cctgataaac
    42961 gtcacttgaa atttgcttat ggggatacga aacataataa tcaattgaga actgtgtatt
    43021 tgctaaaaca ccacctaacg gtgaaaaatt cgacggagcc gccagagtca ggttagcact
    43081 gagactggga cgcatatagt atagttgatc accaatatca aaatcctggc tggcatgaac
    43141 cgttacattg ggagggtaaa tagtaaaagg gtcaccggat tgtcccacga tacctgtttc
    43201 ccaggtataa gtcaaataac ctccccagaa accacgtaaa ggacgtcgta aagtaaactc
    43261 gacgcctttg gcatcagcat aatcagaatt accggatgta tgatactgtt gttcattata
    43321 tgttatgaca gtaatacttc gaacatcgtt agtgcgatcc ttataaaaag cggtaacatc
    43381 cgccaatact ggtccaaaat cctgctcgat acctaattca tacgccactg ttttccgcgg
    43441 gcgcagttca cgattcccca gattacgggg ttcgatactg ccaaattcag gatcaacaat
    43501 ataagttgat aaaatgctat aggtctgctc accttcacca tactcgccga atgatgcacg
    43561 ttggaaaaaa tgaccataat taaaatgtaa gagggatttc tcggttattg gatgtgacac
    43621 cccaattctc ggggataatt gcccaaatgt ataggttggt ttggttttgg gattcggttc
    43681 acgtccttcc atttctgccg taataatatc gaaaggatcg tatggatcga ggtaaataac
    43741 atcattagga ttataaaaat catatcgcag tccgagattc ataattaatc cttcaaattc
    43801 cagcttattt gaccaccaga tgtttccctc ataaggatgg tagattttat cctcaacggc
    43861 ttcccaagca cgcgcctcct gataacggtc aaaagtataa taggtaaagc cgaaaccact
    43921 ccgcatctgc cagtttttat tgatctgacg agtcaattta cccgtaagcg tcaaatcggt
    43981 attgcgatct tccttacgat accagtaacc gatatagtgt aaaccataga tttccgccca
    44041 ggcatcgggt atcgcaaagc cttgcgaatc gtaagctcgt aaataatccc ggcgtatatc
    44101 gctggtggtc cagtctgccg tatcgctcgg aaacttcgtc tggctctcat aattgaatac
    44161 ccgcgataaa tgtagttcgt aaaaagtatt tttatcgaga ttgtgaacaa atttcaaagt
    44221 ctgaccattg cgcctatata cagggtaacc agcttcatca taataagcat acttattacc
    44281 ataatcacct tcgtactcca tcgattccat accgtgacaa gtattatcct gattataata
    44341 tcccgaaaat actaataatt tatttcctcc caaagaatag ctgattttat aagtaccatc
    44401 aagccaacta cccatcttgc gataagcatt acccgtaatc ggtggtgccg tacgataacg
    44461 accggataaa aagaacctaa ggtttttaat cggagtcggt ccaccgaaag aaaattcggt
    44521 ttggtaacca ctccgattgg tgtaatccgt caagggttga tgagtatcta tataaaattg
    44581 ataagcttgt tccggtgtcc aggaacatga cggatcgttg ccgtagcgac cattcggatc
    44641 aacccacata tcgggattat caatccacca ttgcaaatgg cgtgcatgcg tattctccca
    44701 atatcgctct gtcgaataat cgtaaagtgc agttccccag tgatataaac cgggaggaat
    44761 ccagcgaaat tctactgcac cgacataatg atccggtccc tctttagtaa ttatattaaa
    44821 aacacccgat tgagcttcac cgtactcagc atcaaaacca ccggtaataa tttccagttc
    44881 ctcaattgcc atcagattaa cattaaaata aggctgagaa ttcaaaggat ttcgtgcccg
    44941 cattccatca atcataaatg ccacctgatc caacgttccc attcggactg tcaactcacc
    45001 gtagggatta taatcaattc caggcattga ggtaagtata tcggaaagct gagataccgg
    45061 ccgtgacttt atatcagttt cttcaacaac gactcgactc gaagttttat ctaactcgat
    45121 cagaggtctt tcggctacaa ctgtcacggc ttcgccaaat tccaatacgg tctcttctaa
    45181 ctcaaaatct acgcgagtgg tgtggttaac actaacgttt acctcagttt taataacctg
    45241 cttatatcca atcatcgaag cctggagact ataaataccg ggtgcaacat ttatgatata
    45301 ataatcacct tccatatcgg tagcagctcc catttctgtc ccgacgataa taatgttcac
    45361 gccaggtaag ggattgcctg tcttcttatc aataaccttc cctacaatct taccatcact
    45421 cgccgcatat cccaacgaaa taagcaacat aaaaccgagg aaagttcgac taattaatct
    45481 cctgatttgc aaatatgctt tcttggaaat cattatttta tccctacgaa agtttgattt
    45541 tgtcgtttta tttattttac taatcattat tttcaatccc attaaggttc aatgacagct
    45601 ttaataaatc ctttgggctt ttcctgcgcc gcacaaaacg ccgcctcaat ctcatctaat
    45661 ttaaatttat gagttatcaa tggtttcata tttattttct gctcatttaa gagcttgatc
    45721 ccaatttccg ctccgcgtaa aattgtcgcc atatctctaa aatgagcatt gacaatatta
    45781 aaagccatcc aattccaata tcccaaatct ttaataattc tctgccctgg atgatatcca
    45841 aaaataacta acttcccttc catccgtagt aagtcaacgg ctaagtcgag acttgcttga
    45901 ttgccaccga cttcaaatac tacatctgct ccccgaccat tagtcatatc ccaaataata
    45961 gccgggacat cttcttcctg cggattaatg accaaatctg ctcctaacat ttttgcctta
    46021 tcgagaattt cagcgcgaac atcaacgcca ataatccgag aagcaccttt caaagcaatc
    46081 tgttgcatta agatgagtcc cataaatcca gttccgacta aaactaccgt atcgcctaat
    46141 tcaactccgg ctttgattac cccatttgtc gtgcaagcta tcggttcagc catagcttcg
    46201 gcaaaactaa tctgatcagc aatggggaaa atttgattgg atcgaaccgc aacttcttca
    46261 gcgtagccag ctcgatcagt ccagactgca actcgatctc ctcttttaaa tcctttaacg
    46321 gcacttccac atttcagaat ttcaccagac acttcatgac cgatatatcg cggatattcc
    46381 aagttatcga gagtttttgc ccattgatga atttcggaat gacaaactcc gcaggctcgg
    46441 acgcgaacta agcattcatc atcctttata tccggacttt cttttgtttc tattctaaaa
    46501 tcatgggcgc cataaagagc cgcgatgcgc atattattcc tttctttaca tcaatttttc
    46561 aacgaccaaa gtatagtcag gaggggtgga taaaaattta ccacccctcc tttgactttt
    46621 tttacttcat aagtatcatt ttacgggtag aaaccacttt atctgtaact aactggtaga
    46681 aataaatacc actagataaa ctagatccat cccagattac ggaatagaca cccggagaat
    46741 atgtccggtt atctattaaa gtcgcaacct ctttccctaa tacattataa accttcaacg
    46801 taatcggaac acgttcattg acttcgaacc ggatcgtagt agtcggattg aatggattcg
    46861 ggtagttctg ccgcaatgca aacttaactg gcttctcgtt aacttccgcc acatctattc
    46921 cgtttaatcc agtaccgaaa ttcagcagag cccagtattc agaattttgc tgaatatagg
    46981 tggcacacca acccagatct cccggatctg caaacggttc attcggcaca ttcggctgcc
    47041 aggtgtaata tccatacgaa tcacccgtcg tctcacgaga atactccgaa gaaaaactac
    47101 caccaacatt aaatccaata gaagcacttg ccttaacatt gggattgtaa atcgctaact
    47161 ctacatccca taccccagtc tccttattcc actgcgccgc cgatcgtata aacgtcgaag
    47221 gatcaaatat taccgccgag tctaattcag gcacatatac cggataacca tcattaaatg
    47281 ttacccgatc tcccattatc atcaaatgat aaggaccatc cggtgccgcc atcggattcc
    47341 cttcccagtt atcccaagat tccactccta aacggttcga aacccctata aataactggt
    47401 cactcgccca atgtccagat gtgtcagtcg gatccaaaaa ttgaccaccc cagtacaaat
    47461 ccgtcgtgtc attgacaaac tcatcaatat gcataaatac atatagagta tcatccgtcc
    47521 acaacaaacg cccatacaac tcatcatact ccggctcggc tatctcacga ccataagaac
    47581 tcgcccagat attgtaaccc gtctcagtca ctaaattcac ttccgccgcg ttaccccaaa
    47641 ccgactcatc cattactcca tcaatcacta catccgcagg tgataccttc ggaacattca
    47701 actctaaacg aacaattacg tcgtccggtc ccggtagaaa attcagcaga gcccagtatt
    47761 cagaattttg ctgaatatag gtggcacacc aacccagatc tcccggatct gcaaacggtt
    47821 cattcggcac attcggctgc caggtgtaat atccatacga atcacccgtc gtctcacgag
    47881 aatactccga agaaaaacta ccaccaacat taaatccaat agaagcactt gccttaacat
    47941 tgggattgta aatcgctaac tctacatccc ataccccagt ctccttattc cactgcgccg
    48001 ccgatcgtat aaacgtcgaa ggatcaaata ttaccgccga gtctaattca ggcacatata
    48061 ccggataacc atcattaaat gttacccgat ctcccattat catcaaatga taaggaccat
    48121 ccggtgccgc catcggattc ccttcccagt tatcccaaga ttccactcct aaacggttcg
    48181 aaacccctat aaataactgg tcactcgccc aatgtccaga tgtgtcagtc ggatccaaaa
    48241 attgaccacc ccagtacaaa tccgtcgtgt cattgacaaa ctcatcaata tgcataaata
    48301 catatagagt atcatccgtc cacaacaaac gcccatacaa ctcatcatac tccggctcgg
    48361 ctatctcacg accataagaa ctcgcccaga tattgtaacc cgtctcagtc actaaattca
    48421 cttccgccgc gttaccccaa accgactcat ccattactcc atcaatcact acatccgcag
    48481 gtgatacctt cggaacattc aactctaaac gaacaatctc ttcttggctt aatgccaccg
    48541 attgtaccgc cactaatagc aataatgcta acaaacacat attaacctta cgcatactta
    48601 cctccattcg gttaataaat tggttaataa ttccttgtct tgttttacaa aaataaagta
    48661 agcctgactt ataaattaca cctcctttca tttgtttttt tacctttctc ataattgata
    48721 ctcaacgttt attgatggaa taattgtttt agtatcatat ttaccataaa aatattcatc
    48781 ctggcttttc ttgatatctt tagcaactcc cacagaataa gcaatactga gatctagata
    48841 aaattggtta aataaaaatc caataccagc cgatattcta tgttgattta cattcggcaa
    48901 taaaagacta taactacgat ctgaatttgg ggttgtctga taattatatc caatccggta
    48961 cttcaaatta ttctcgcctg aatattccag tccgatagca aatacaaaac tattacgatc
    49021 gtaaaacggc atttccgaac gccagtaacc ggtgatacta tcacgcggag ccgcagctat
    49081 tatggcagac caggcagtgt cactataatt aaaatccagt ttggattgag tgcttcccca
    49141 gaattcgctg gatagatcaa tatttagatt tagttgatcg gtgggtttgt ataaaattcc
    49201 tataccacca gtcatcggca tttgaaaacg actgtgaagt cgcgatttct tcgctagact
    49261 atcaagcgca tagaagcaat ccgttttagc attaccttca aggtcaaaat ctacgccgct
    49321 ccgaatgctc ccgcctacct taatcgtcgt tgttataggt aaaataaatc ctaggctcca
    49381 acctatggca ttatcctctt gttctaaatt caaagcgtag accggattag tcctgttcgt
    49441 ctgccactca ggattcgaaa tcggaaactc aaattgttgg taaatctgat aaatattaat
    49501 cgcggcaccc aatgacattt tcccgacttg taatccgaaa acctgcgaat aggcatccgc
    49561 aatcaggcgt gattttaatt gaaagccata caaacgggtt attccatttt cagtaaacat
    49621 taatgccagt ggccatcgaa ccgaataatc aagggctcga ttatagacca tagccagccc
    49681 aaacttttgg ttaggcaccc agtaaatacc cgctgaaccg ctgaaatcat tgaataaata
    49741 ggaacgatgg atatcacgac ttcctttttg acattgttgt tgcccggctc gatcaatgat
    49801 attcatcgcc agaccaaaac ccggtgtgct taaaatttta cccggatttt gaaacgtagt
    49861 tgttatacca tctaatccgg cataattata accccccaaa gctagggatt ttgcactggt
    49921 tattggagca gtactcgcat ctccggcttt tgtaaaatcc gaaagaatca aaaccattga
    49981 aattgtccaa aagataattt tttttctcac ttagaatttc ctcttcaata aaactctttc
    50041 aaatcaccaa gtattaaagg ttatatatga atgatactta gtggatctta agtgatcaac
    50101 cccattccaa tccttaccag ccagccagaa taaataggtc ttattgcggg acaaaccttt
    50161 tgcaggatat tcaacaaata ccttcgtccc gggaatcggg cttcctatta ccagcggctg
    50221 acctattaca ttgttttttc catatatctt ttgaccacct acggtatcca ccgaccaggc
    50281 ttcccagact atttttttgg ggctatagct ctcacctaaa cagattccga ccgccgctac
    50341 gagagaatct tcaacgcttg aatcaataat ctcccaggtt atgaccggtg aattgctggt
    50401 atccgtttca ataagagtga ttttggcaaa tccacccctc aacttaatgc ttgccatatc
    50461 aacatttgta taaacatcta tccagcaact cttttgaaca tgactgtaac tgtctatcca
    50521 taaagtatcc tcattggcaa tcgtcgttac cgtgtccagt tctgccatta agattacttt
    50581 accggcattt tctactacgg catcccaagc ggcttcctta ataacccaaa atgtataggt
    50641 gctgtcttca attaatgctt caaaatgtcc accataatca tttgttatat cagttacccc
    50701 ctcgggcaat tcgccatatt taaccgggta gtgcaaattg tcaccatcaa cctttattaa
    50761 ccattgcaaa gtactgtcaa gccgtgcttg atcgccgcga tttactccaa aagcacaccc
    50821 atatccaccc aaccaagtaa atttgggcgt aaatgtttcc tgttcaataa taatattgtt
    50881 cattggccgc gcgccttgat acggagccag cgtattcttc tccccacagg accttaaaag
    50941 gcaaagtaaa aatagaatta aaatgctact tgccgaaata aagtggtttg gtttaaataa
    51001 gtaattcata aaataaacct tatgctgttt ttactaataa ttattattag cctcttctta
    51061 atcacgatac tatcctttaa ttccacttaa agtgatacct tttatgaaat atttttgagc
    51121 gaagaaaaag acgattaaaa ttggtaaagt tgccattacc gaaccagcca ttaataaatc
    51181 ccagtctgta atatatcggt cgcggaatat agctagtcct aactgaaccg tgcgtaaatg
    51241 atctgaattg accacgatta aagcccagat aaaatcatta aaagtcccca taaatgtaaa
    51301 aatggctaat gttgccagag ccggtttcga taaaggaata attatctgcc acaatactct
    51361 aaattttcca cagccatcaa tataggccgc ctcttctaat tccttaggaa tcgtcaagaa
    51421 aaattgtctc aataaaaatg ttccaaacgc cgaagccaag cccggaacaa tcatagcttg
    51481 atagctgtca acccatccga gccagtataa aaccataaaa ctaggaatca tcgttacctg
    51541 gtaaggaatc atcatagttc ctaaaaaaat atagaaaaga acatcccgtc ctttgaattg
    51601 taaccgagca aaagcataag ccgccatcga acaagtaatt agctgcccta acgtcacagt
    51661 acaagctact ataagactat ttaaataata tcgcccaaag ggagccgctt gaaaagcccg
    51721 ggcataattc tcaaaatgga actttgaagg tagccacttt ggtggataca caaatatatc
    51781 cccaatatct tttaccgaag ttaggagcat ccagataaaa ggtgctatcg tcgccaaagc
    51841 taaagcgtat ataataacat gaaggataat gtcttttact atatgactat ttttccgaaa
    51901 atctatagcc attataccgc ctcatacccg gcacgagttt gcataaactt tacttgtaat
    51961 accgttagca ccgctaatat tgcaaagaaa acataagccg ccgcagaggc ataccccatc
    52021 tcgaaaaatt tgaaggcatt ctcataaata taaaatacca tcaccttcgt tgcgcccaga
    52081 ggtccccctg aagtcaaaac atagacaata tcaaagacct gaaacgagct gatcattgac
    52141 attatcataa tgaaaaatgt ggtaggactt aacatcggca ttgtaatatt ccaaaatttc
    52201 gaccaccatc cggcgccatc aatttcggcg gcttcatagt aatgatcggg aatcccttgt
    52261 aatccggcgg aaaataaaac catatttgtc ccaaatgttt tccacactcc catgataata
    52321 agagcaacca tagcggcttt cggttcgttg agccaattta ccgagggaag ccccacgaga
    52381 tccattaaat agtttaacaa accaaaattg gggtcataaa tccaacgcca gattaacgca
    52441 gccgctaccg gggatataac cacaggagtg aaaatcgccg tacgtaaaaa ccgttttcct
    52501 gcgatgcgtc ggttaagtat aatagcagtt aacagagcaa ttaccatatt taaaggaacc
    52561 gtaccaagtg tgtaaataaa agtatttttc agaaccatcc agaattcagg atcagaaaat
    52621 aaccgacgat agttctctaa accaaggaat tcccgcccac tactgaacat attccattta
    52681 tgaaaactga gataaaacga gaagaatacc gggaataaaa cgaaagcgca gaaaataacc
    52741 aacgtcggag ataagaatcc gagcgctgtg agaatattct ttttactatt cccatcccgg
    52801 cgatttaaaa tcgtcttttt aactctggta gatttattta gcattctgcc aatacgattg
    52861 taagatctta ttagatttcg cagccgcttt atccagagct gcctttggat caccgccacc
    52921 aagtgttgct tcttcaatgg cttccgccat atgtcgagta atttgtaaag ccccgtaatc
    52981 catagaacgc tgaatctggg caacttccat ctggtctaca aatactttaa aattcgggtt
    53041 ttctattaga tatttcttga actccggtat ttctgatact gaccgccgca ccggcaaata
    53101 accggatttc atagcccagc gagcttgaat atcaggccga atgatccact tcacaaatga
    53161 ccaggcttca tcgggatgtt cagattgttt gaaaataacc agatattcgc caccgcagat
    53221 ggtcgcctgt tttacaggac ctttgggcaa cggtgcaaac gaccaattca aatttttcag
    53281 cattcgatta aatcgcggga ggttccaagg tccatccatc gccatagcca ccaacttcga
    53341 ggcaaaagat acatcgtaat ccgtagtaaa tgtccgtaaa ttcaatttgt catatatgtt
    53401 tttccagagt gttagagctt taactcccgc ttcactatta tataacactt tggattgatc
    53461 ttcagctata atataaccac ccgcttgcca taaataggga atccactgcc aaaccatcca
    53521 gccacttaga gggcccgcgg ctggaaatat gggcagaaaa aatccgatcc ggtcaaattt
    53581 cccgtcatta ttaatatctt tagataatcg cacggcatat tcttctaact cttcccaatt
    53641 cgagggagca cgaccatcct gatagccagc ttctttgacc atatcaacat tatatatcaa
    53701 cgccaggtta gtggcttcca ttggaataca atatatcttc ccccgcagag aagaatattg
    53761 taataaagca ggataaatat cttctatttc agatttacta agtccattgt tgcttttgat
    53821 aaaatgatcc atctcgtaaa tagcatccgc atcaaccaaa tcagccagaa aatcagagtg
    53881 aatccaggaa atatcaggcg cggtcttact ctgaattgcc gtgataagct tttgaattag
    53941 agcatcacca gttggcacat attgagcttt tattttgaca ttgggatttt cagtttcata
    54001 ttgttcgatt aaatcctcga gcgctggtat agtagatgcg acgaagctat gccagaaggt
    54061 gatggtaatt ttatcatctt tagtagtttc ttttttccca catccaataa atactaataa
    54121 gcaaaacgat agtaaaaaat atctactcca tcccctcaga ttggttgtaa aaacgtttgc
    54181 gcatttctgg ggtgagtgtt gattgccgat atttttgaat tgcatagttc ggacctaatt
    54241 tttattggtt ttaatcggga ttaataacag aatttagatt tcggacggat tttcgaatat
    54301 tgaggacagg ttttaattcg atcctggttt tctcttttct tcccttactt tttaaatcgt
    54361 catacaacaa tttaaccgcc atctcaccca tgatctgccg aggttgctga atagccgtca
    54421 gcggggtaaa aagatacggg gcaaaatcta catcatcaaa agaaatcagc gaaacatctt
    54481 gagggacatt caatttttct tcgataattg catctaaggt acccaaagta attaagtcgc
    54541 ttgtactgaa tatcgccgta ggtggattgg acattttcaa tagtagtttg gtctcaatat
    54601 atccattttc tcttcggtaa tcgttaccaa cgattaaatt ctcatcgatt tcgatattat
    54661 attttataag ggcattttta taacctttaa cccgcatatt gttagtagaa gtattgggaa
    54721 gcccttgtat aattgcgatc cttttatgac catttttgat taaatattca acagctttat
    54781 aggaaccagt atagttatct acaaccacag aattggtatc aatggaatca aaacacctat
    54841 caagtagaac caaaggaatt ttttcatcaa ttagttcctg gatgtgatct gattttaacc
    54901 ccaccggcat tataataaaa ccatctactc ccttgctacg cagtaattgt atttgttcaa
    54961 tttcagtatc ttgattttca tcagtattgc acacaatcaa agtataacca agtttataag
    55021 attcaatttg aataatgcgc gatacatgag caaagaaggg gtttgaaata tctggaatta
    55081 ccaatccaat agcatgcgtc ttccgcagtc ttaaaccacg ggctaattga ttcgggacat
    55141 aattgagttc ttgagcaatt ttgtgaattt tatcggcagt ttcatcttta atccggttta
    55201 ctttcccttt attattcaat acccgagaaa ctgttgaaac ggaatatcct gattttttgg
    55261 caacatcttc gagggtcgct gtcattttga aaccactttg taaatctgcg caatcatttt
    55321 cgcaataagt atagtaaaga aaaaaatatt tgtcaagcta atttttcaga gtattacgaa
    55381 atttttctac gttcatctaa taaatcattg atatcattca atatatagta ttttaaattt
    55441 attgttacat acctttaaat cgcgacaatc tggggatgat tttctacctg ccgctaataa
    55501 gtcgagtcag attaatattg tttttcttct aaccgctaag agcgctaagt ttttatcaat
    55561 gataagtcca ccagccaaac ttatactcgt cagggcaatg agtgttaacc acaaaaaata
    55621 ctaaaagaca catagaactg cataaatgct tttttgcata attgattgtg gtaaatggtt
    55681 actgttagct ggttttggtt atcggtctat tgtcagtaaa ctatttataa acccgattct
    55741 attattcgaa ttctaataaa actatcttgt taatcttgtc taaaatatat cccacgcctt
    55801 agcgcgtggt tcgtggtcaa tagttaacat accattttat tttaggacga tgctgacgat
    55861 tttattcttt accacaaccg tcttaacaat cgtatgccct tcaaggtaac gctggacatt
    55921 atcaatctgc agagctttgg caatgcaaac ttgatcctca gaatcacgtt caatatcgaa
    55981 agtaccgcga attttaccat taacctgcac cgccattgta acgagatcat caataattaa
    56041 actcgggtcg taagttggcc agtcaagata tgccagactt tgctcattgc ccattagatt
    56101 ccagatatgt tcgctaatat gaggcgcaaa tggattcaat aaatgcagaa aagtcttcat
    56161 taccgatagt ggtttaaccg tcaatttggc gatttcattg accaagatca tcatctggga
    56221 gatggcggta ttaaagcgca gattctcaat gtcatccgta atcttgaata tggttttatg
    56281 taaaatagaa ttagtcgaag catcgggcgt aatatcttta atatccgggc tgattaaacc
    56341 ttcttccgtt acgtaaagat tccagacgcg atttaaaaat cgaaaaactc catcaatgct
    56401 ggaagtattc cagggcttaa cacgtgtcag cggtcccata aacatttcat acatgcgaaa
    56461 agtatccgcg ccataggtat ctatgatttc atctgggtta atgacattac cgcgtgactt
    56521 gctcattttc tcgccatctt cacccagaat cataccctga ttgactaatt tcataaaagg
    56581 ttcaggcgtc gagacatatc ccaaatcgta taatacttta tgccagaaac gagcgtatag
    56641 caaatgcaaa acggcgtgtt cggcaccacc gatatataaa tcaaccggca tccataactt
    56701 ttctttttcc ctgtcgcaaa acgtttcatc gttgtgagga tcgataaaac gcaaatagta
    56761 ccagcacgat cctgcccatt gcggcatcgt gttagtatcg tagcggcaat gttttccggt
    56821 ttctggatcg ataaaatcaa cccattctgg cagattagct agcggagact caccggttcc
    56881 actcggttcg tatttttcca gaggtggcaa ttccagcggc aatttcgatt catcaatcag
    56941 gtgaatattg ttatcttcat cataatataa aggaaatggc tcaccccaat aacgttgacg
    57001 ggaaaatacc caatcacgca atttatattg aactcgcgat tgtccaattc ccttttcatt
    57061 taaccactca ataattcggc gtttagcctc agccacattt agtccattca gaaatcccga
    57121 attaactaat atgccgtcgc cgacataagc ttctttggta atatcgcccc cggatatgac
    57181 ctcgataatc tcaagtccga atttcttagc aaattcccag tcacgttgat cgtgtccggg
    57241 aaccgccatt atcgctccgg ttccatacga cattaacaca taatcggaag tccagatcgg
    57301 aattcggcga ttattggctg gattaattgc atataaaccg gtaaatacgc cggttttctc
    57361 cttcgctaat tcggtgcggt ccaggtcact tttggaagcg gccgcttgtt gataagcttc
    57421 tactgttttc ttgtattccg gtggaactaa agtcttcaag atcggatgtt cgggcgccag
    57481 aaccatataa gtagccccaa ataaagtatc cggtcgagta gtgaagactt tgatagtctg
    57541 atccgaacct tcgattttaa aatctatatc ggcgccttcg ctccgcccga tccagttccg
    57601 ttgcatttcc ttaatacttt cggggaaatc aaccagatcc aggtcttcca gaaggcgatc
    57661 agcataagcg gtgattttca acatccactg ccgcatcgga atccgaatga cgggataccc
    57721 accgcgctcg gatttgccgt ttatgacttc ttcattagcc agaacggttt tcagttccgg
    57781 gcaccagttg accggcactt ccgccaggta agccaaaccc attttgtaga tttgctgaaa
    57841 aatccactga gtccatttgt agtattttgg gtcagtcgtg ttaatttcgc gatcccagtc
    57901 ataggaaaat ccaaatgatt taatttgtcg tcgaaaggta ttgatatttc tttccgtagt
    57961 tattttcgga tgagttccgg tttggagcgc ataattctca gccggtaatc cgaaagcgtc
    58021 ccatcccatc ggatgcaaaa cgttgaaacc ttttttcctc atataacgag cgattatatc
    58081 ggaagcgata taaccttccg gatgcccgac gtgcagaccg gctcccgatg gataaggaaa
    58141 catatccagc acgtagtatt ttggtttatt agatttatca ggggttttaa aagtcttgtt
    58201 atcttcccaa tatctctgcc atttcagttc aatttctttg aaaggataca ttaattactc
    58261 gttctaaatt tggaaatatc agtataaaac tctgaacttg attgtatttg gtatattcat
    58321 tatttcaagg agcacttcat tatcgtgatt tttttcaata tcggttatca cgtaaccgat
    58381 atcctcggta gtttttaaat attgtccaac gatattaata ccatgcctgg ctaaaatgtg
    58441 attgatttgg gacaatatgc cagggacatt attgtggacg tgtataaggc ggtgggcgcc
    58501 attgaattgg ggcaattgca aattgggaaa attcacactc ataatcgtat tgcccgagtt
    58561 gataaagcta ataattttcg agggtacgaa ttgagcaata ttttcctggg cttcctcggt
    58621 atttccgcca atatgcggtg taatgattgt attcggcaaa tggcacaatt gacaataaaa
    58681 tctttcctga ttatttaacg gttcagttgg aaaaacatca atagccgctc ctctgacctg
    58741 accactttga aggttttcag ctaaagcttc aatatccaca acgtgaccac gactgaggtt
    58801 gagaaataaa acgcccggtt tcatcttctt aaattgctcg gcgccgatca gatggtaatt
    58861 ttcttcacgg ccatccacgt gaattgaaat tacatcggct atagccaaaa gttcatcgag
    58921 agaattgcat ttttttgcat tgccgagcgc taatttttcg acgatatcat aatagtaaac
    58981 ctgcattccc atatcttcgg ctaatattga aagttgcgct ccaatttttc cataaccgac
    59041 tatgccgatt ttctttccac gcacctcgta gcagtttttg gaatttttat cccaaatccc
    59101 atcgtgaagt ttctgacttt tgtcgaaaat gccacgaaat agcataatga tctctccgat
    59161 agccaattct accacactgc gcgtattgct aaagggagca ttaaaaacag ctattccttt
    59221 ctgggaacag gctttcaggt ctatctgatt agtaccgatg caaaatgctc caatagccat
    59281 caaattttgg gcattttcca gtatttcggc atcgataatt gtcttcgagc ggattccaag
    59341 aactgagatg tctttgatct tctcctttaa aacttcatga ggaatacttc cggacagcga
    59401 ttcaaccgga aaaccttcgt tctgaaaaat acgctgtgcc gtcggagaaa tattttccag
    59461 taataataca ttggttttac ttttctgaaa tgttacatcc atattattat ccattttttc
    59521 aggctcaaat ttaaaacaat ttaatacttt ttcaccgttt gagtgaatcc aattttaaat
    59581 atttttttaa ttattaaaat agcttagcag ttaaagaatt taactcacta agtatttttc
    59641 tgtgatttcc gataataaat tgggtagatt gatcaatata tctggcgaga tttgcgtcaa
    59701 tgtcagccgg taattcattt tcatcgagca ggcttatttc accttgcggc gaaataaaaa
    59761 catccacgat taaatcacgc cattcaaact gaaaattact caggtgaatt cggtcagcaa
    59821 tattaaagta atcgccgaga tggccaccct gcggagcaaa ccaccgatat aaagtaaagg
    59881 gttgcgattc ccaatagaaa gcataagtct gcgtattttt cggcaaaatt tgattattga
    59941 caacgtatgg tttttcaagt atgtatctta atatcccgaa atcttcatcc agaaaaatca
    60001 actcgcagtc aaaagtccgg acttcaccgg ataataaatg ttttacttcg cgacaggcag
    60061 gataaatcat tttattttcg ttcaggcgac aagatgctca attaaaattt taaaaccaat
    60121 ggcaatcagg ataattcctc cgactacttc gactttttta ccaaggtaat gtccaattcg
    60181 tttgccaatt attattcccc ccaaagaaat aagaaacgtg ataactccaa taatgatgat
    60241 gggtacaaca atattaattt ctagaaaagc aaaggtaatt ccgaccgcca gagcgtcaat
    60301 gctggttgcc agcgataaac caaatagacg atgactattc aatggatcga ttgcattggt
    60361 acattctttt ttcgagaaag cctcataaat catccgtatt ccaatgaaaa ccaatagccc
    60421 aaaagcaacc caatggtcga aattcgccag aaatctctgg agagtttggc ctgccagcca
    60481 gccgatcagc ggcattaaag cctgaaaacc gccgaaggct aaagggactt ttaaaaactt
    60541 gaacttatta tgaggctgag ctgctaaacc gcaggtaatc gaaatcgcaa aagcatccat
    60601 cgccagacca attgcaatta atattaatgc taataaattc aattattcct gctccgactc
    60661 ttcttcgtta atcacattaa gatacttaac ttcctgttcc ggcggcagct cggatttttc
    60721 tcccagctcg actttggcaa ttttctcgaa tgaattggaa attttattgg aagttgtatg
    60781 aatgtccttg acatcttttt gaaccgtgtc tatatgagcg gataatttat tccagcggtc
    60841 gcgataacga cggaactcct cggataattt cataatttcc tgttggataa tgtcggcata
    60901 tttgcggcgc tctatgtcac gcaagacgag ttgaaccgta ttcaaaaccg ccataaaagt
    60961 agtaggcgaa gtcaaccaaa ctcgacgaga atgagaatat tctattaaat cgtcatgata
    61021 agcatataat tcggcaaaaa ctgcttcggc tgggataaac ataatcgcct ggttggaagt
    61081 ttcgcccggc aaaatgtact tatcggcaat ctcgtcaatc tgcttcttca cgtcagcttt
    61141 gaacaacttt gttgccgtaa tacgttcagc ttcggacagg ctcttgtcaa tcgagcgttt
    61201 gtaattatcc agtgtgaatt tggcgtcaat tggaatattg ccgattggtt ccggtaaaaa
    61261 caagattgca tccacagttt tgtcgttgct aagcgtatat tgaatctgga atattttatc
    61321 gttcttttcg ccaaaaatag catagaggac ctggttgagc tgcacttcac caaatatgcc
    61381 ccggcttttt ttatcactaa gaatttcctg aagtgagacg acattagtcg agaggctttc
    61441 gatatttttc tgagcttcat caatctttgc caatcgctcg atcacattgg taaatgtttc
    61501 atttgttttt ttgaacccct cactgagatt ttcctgaact ttattattga tcgcatctaa
    61561 tttttcggcg acgtttttgg tcaaatgctc aaaatcttca gataccgccc ttttgaaagt
    61621 atctttgaac tgactgaatt cttcagtctg ctttttactg ttctcgctga gcgatttggt
    61681 gatttctact tgaaagttgc caaacttctc aatcatctca cgatttttgg tagaaacatt
    61741 gtcggtaaat tcggtaaaac gcttttggac aatgtcagcc gttttagact gaactaattc
    61801 cgggaaaatg ttcaactgcg attccagggc aataattttt tcttttaaag cttccagagc
    61861 ggcatcgccg gattgatccg tcttttttct cacttttgcc attataatta ccagtaagct
    61921 gataataact aagccaccga ttatgataaa taagtctgtg ttcataacta ttgcctttta
    61981 attttccgtt gaacggattt tattgaaaaa tgaatttagt aaagatcggc ataattcagc
    62041 tttaactccg cctctcacca aagccttatg attgagcagg ttttggtcgc aaaggttttc
    62101 gaccgaaccg cacattccag ccgcattgtc gtaagcaccg aaaacgactc gtgaaattcg
    62161 agcatttaag atagccccgg cacacatcgg acaaggttct agagtaacat ataaagtaca
    62221 atcccctaac tgccaggaat ctaaaaaagt agcagccgag gtgatcgcca acatttcagc
    62281 atgggcggtt gggtctttca agccttcccg ctggttatga cctttaccga tgattttatc
    62341 ggcgtgaacg acgatggcac caaccggaat ttctccggca tccagtgcct tttcagcctc
    62401 ctttatcgcg aaatccatcc aatattcatc ggaattcaca atcaaaccca tactaaaatt
    62461 taagtaatct ttccgacgaa aaataaccat atccggactt tcacttattt tttcaataaa
    62521 atgccggtta atcggaatgc gatttacctc cagcatgctg tcaagtttgg gaatggcttg
    62581 tttttatttt ttcaacacaa agacccgaag gcacaaagat ttttgcactt ttctagaata
    62641 tcctggtatt caataagata ttacgtctat aaattaattt ttagaggaag ttctgcaacc
    62701 atatcagttg attaattgca tctttttccg tataattcgg ctgtaaaatt tgggaaattc
    62761 gattaaacat tcagtaacaa aaactcatct tcgacattta ataattaaaa caaactacac
    62821 ttagtctaaa aattggatta attaactata acatcaatga aattaaggaa ataatgatgg
    62881 acgatttaca attgcaacag aaattatcag cgacggcttt aaacgccgcg ctcgaggcgg
    62941 gaaaaattct caaacaaaaa gtcaataccg ccttgaaaat tgactataaa ggacgtgtca
    63001 atctaattac cgaagctgat cgtatgtcgg aaaattctat tatcgaggca atccgccgtc
    63061 aatttcccga gcatcagatt ctggcggaag aaagtccgct gatagaaact tcttccgatt
    63121 acaaatggat catagatccg ctggatggta caaccaattt tgtgcacggt tttgcattct
    63181 tttctgtttc aattggacta gaatatcaaa aagaattgtt attaggcgtt gtccacgctc
    63241 ctttgctggg cgaaactttt accgccatga aaaatcaggg tgcctttctt aacggacaac
    63301 ctattcgagt ttcgcgggta agaaaacttg ctgatagtct tcttgctacc ggtttccctt
    63361 acgaaaataa cgccacgttc cggcgcaatt tcgagcattt ccggcgcatt tacccgctta
    63421 ctcagggcgt tcggcgagtc ggttcggcgg caattgattt gtgttatact gcctgtggcc
    63481 gctttgatgg attctgggag ttggatttaa acgcctggga cgtagccgct ggagcgctaa
    63541 ttctccaaga agccggtggc aaaacggtaa atatggacgg ttcggcattt tcgatttatg
    63601 cccgccagat tttagcgacg aatggtttaa tcgaagccga attattgccg attttcaaag
    63661 aataaattct aattagttac ttcagtcgaa ccatttttcc gaatggcgac ctcatgaatc
    63721 acgcgttgag aagcgcgtgt taccgtcaat gcaacagatg gcagaattaa tctttctcca
    63781 ggacgcggaa ttttttgcag atgagcaata atgagcccgc cgatagtatc gtaatcgccc
    63841 tctggcaaat tcagtccaaa tttgtcattg agttcctgaa tttccatttt gccagagaca
    63901 acaatcgtcc catctttttt aattttatag cgaggacgga catgatcata ttcatcttct
    63961 aatttgccga caacctcttc cagaatgtcc tcaatagtca ctatgccctc cgcgccgcca
    64021 tattcgtcga caattattgc catattgttg ccgctttttt tcatttgatc gaacaaaacg
    64081 tttatgctca tattgggtgg tgcataaaaa gccggacgta taaatctcct gattggagta
    64141 ttgttatttt caccgatgac atcgaaacta ttcaatatgc caatgatatt gtcaacccgt
    64201 tcacgataca ccggcagacg acgatgctta ctttcggaaa tgatgcgcgc cgcttccgga
    64261 acgctgtcgt cctcaggcag agcagtaact tgaatgagcg gcaccattac atcttcagcc
    64321 tttttttctt taaatgataa aagtcggccg atcatttcac gttcattagg attaacgtca
    64381 ctcttatcgc ttattttcat taggattttc agctcttctt tactgataaa tggcaatttg
    64441 tggctgagtt cgccgccgag aatggtagcg atataagaag cggctcccga aaaaacacga
    64501 accaatggtg taaatataag agacattatc ctcaatacag gcgctatttt cggagcaatt
    64561 acatcggcat attgctggaa aacgctttta ggaacgattt cagcaaaaat ccaattgatc
    64621 aaactcatac aaaaaattgc gacgaatact ccggcaccat gaaaatatct ataggctata
    64681 gccgttgcca gcatcgtgct ggtcactgtg caaatattcg tgccgatcaa ggtcgtcccc
    64741 aaaacccatt ccggtcgttt taaaagattt atgactaact tagcatcttt tttgcccatt
    64801 ttggcatcgt gacggagacg gattcgatcc acggatacca gtgcaatttc cgaaccggag
    64861 aaaaaccctt ccccgagcac acaaatagct ataattagaa ttgccaaacc ggtcggtatc
    64921 acaatcgctt cacccgcaag atttcgatac ggttattttc tattttctca acggtgaata
    64981 gccagccgtc gtaaatcttt tgagcattaa cagtaggtaa ttcatcaaat aaactggata
    65041 caaatccacc gatagtctca aattcatcta ccggaattct ggcgttcaat aattcattaa
    65101 aactaactat gggcagagta ccacgcactc gatagagccg atcgttaatt ttctcaaatt
    65161 gctcatcttt tatttcgaat tcgtccgaaa tttcaccgaa aatttcttcc agtaaatcct
    65221 caatggtaat caagccttgc actatgccgt cttcatcccg aacaatagcc attgaaattc
    65281 gctttctttg aaaaatatgg aacagttcat ccactcgttt ggttttgggt acaaaaaacg
    65341 gtgggcgcag aagattccga agatttatcg gcttagtttc atcgttactg gcattcagcg
    65401 aaatgcctat taaatcagta gcaaataata ctccgataat attgtcagga ttgccttcat
    65461 aaatcgggac tttagatata cgcctattct tgatggcggt gataacttcc ggcaacgcca
    65521 tatcaaccgg cagactaaaa attaaactgc ggggtgtcat aacatcggcg gcaaccaaat
    65581 cgccaaaatc gaatacatta gaaatcattt cactctcatc ggaatcaata acgccttctt
    65641 tttcactgtc ttccaatatt gagcgaataa catcttcagt caaaatattt tcgcgctgat
    65701 ctcttttcga tacgatcaga ttaacgattc ggtcggaaat attcctgata aaccaacgta
    65761 atggcgtaaa aattaccata accagcgaaa gcggtcgggc aaattttagg gacaaacgtt
    65821 cgttattctt gatagccaag gtcttaggcg ttatttcacc tatcagcaga actagcggca
    65881 aagcaatcaa ataatttgcc cacggcaaaa ccgttccggc aatctggata acaatcagag
    65941 taaacaaaga tgaaaaacaa atattaacaa attcattacc cagaagaatc gtaataatca
    66001 gcgagcgggg tgtattaagg agatcgttga ttattttacc ctgggcggga tcgctctctt
    66061 gaattttctc acgttgaatt ttcgacaagg aaaaaagagc cgtctccgag ctggagaaga
    66121 atccggacaa ccccagcaaa acaaccagta taattatttc aatcgtaacc aaaaccacaa
    66181 attaatataa taaatagcgg tgatataaaa taccaaccag aaaattttta cttcaattta
    66241 cttctaataa tatagattga gagataaacc gacagtattt ccggataaat ctatgtgaga
    66301 aatgctggca tctttaaagc gttgcgtggc tttgttttct atattcatta atttatattt
    66361 catattataa tgattccagt cgcatgctaa tccaataaat ttgaatgggc ggagttcggc
    66421 gccaactccg tacattaatc cccagctgtg aaaaatcgga ctgcccattg atttattatc
    66481 ttcaactgca tctatgatta gcacatcata accgaacccg acattgcaaa acggataaaa
    66541 taaaggaaaa cgaataggcg ccaaacgcaa attcagattt accgacggca tccagaatat
    66601 taaagtcggc tctgaatagg tagcatcaaa attcttaatc cattgggtaa aactactgcg
    66661 atagatgccc aagccgaaat caaaaccaac tacttcatcc gattgcctta aattggataa
    66721 ttcgatgcct gagaaattgt taatctcatc aaaaacgtaa gctccgggtt gtctgcgatg
    66781 aaaaagttcg ttgccctccg ccattttggc attcattatc tgtacttgat tattgatttt
    66841 atcaacaata tgaaagtcag aataacgaat tttaaaacct gttttaaatt gacgtttcgg
    66901 cttttcggct acattctttg taaccggttg gagtttttta gaaagagcaa cgttaatttc
    66961 cagtacttga tcctttttta tatcgtaata atgttcccat ttatcatagc cttcgttaac
    67021 aatggtcaat ctatgagtaa cgctggggac aacttcaact tcgatgggag caattccata
    67081 gtaaacatca tctataaata ctgaagcgcc ataaggttca gtgcttattt tgaaggctgt
    67141 ttttttacct tttaattctt tagtctgacg ctcgaggcca gaaatttgac gagctacaga
    67201 tggaattgct tcggtcaaaa ctgctccaag tgctccctca atgtcttcaa tagccgtggc
    67261 ttcgatccgg ctggtttcga catcaattaa tcggatgctc accgaataaa tttcaccgac
    67321 tttgctgacg gaaccagcga taattttctt gacgcctacg atttttccga cttcgacgac
    67381 acattcatcg gaagtacaac cactcatttg aaattgcatt tcatcgagaa ttttattcat
    67441 cttatcgcgt tccataacct ggaaaatacc ggcgttgaat agctcgattc gtaagcgatc
    67501 agtcagcgct tttgcttccg ccaaagtaac gccattagca tctaattcca taactgcaat
    67561 ggcgattttc tccgcctctg gaaagtcaga atattcaact ggaatccctt tagtccaaat
    67621 agtgtcttta tgagcatcta atttatactt ttctccacta tattcttgag attcagccgt
    67681 tgccaataga caaaaaaccg atgttaccac tacaattaag tcggttatat tgaacatttc
    67741 ctcatcataa ataaatcgta tatcctcaaa attttcgggt aatataaccg caatcggtat
    67801 ttgtagatat gatatttctc acacttttta gtgctatgat tcatcgaatc tcgagttttc
    67861 tcaattgtat ttaatctttc ctatcccttg attctaaaaa tccaaggtta gacatttata
    67921 acctaccgcc tcttatattc tgaatattat tggcaatatt tacttgatta acaaacactt
    67981 tcgagcttcg ataaaatcac cggcttgaat gcgatagaag taaatgccag atgaaaatct
    68041 cgatgcgtcc caggttattt ggtaattgcc aggctcatgt gacctgtcaa ccaacgtctc
    68101 gagcaaatta ccatttacgt caatcacctg aattttaacc ggtgatttgg tcggtattga
    68161 atatttaata ttagtggatg aattgaaagg attggggtag ttatggcaaa gtttataaca
    68221 atccgggagt ttcttgaaaa attcagtaat ggcactaaat cttttatatt tatataagct
    68281 gttggcttta ttcaaaacga aggtggcatt atttgttgct gaagtaatca ccgccaaagc
    68341 tgtatcttgt gaaattgtac ttgataacca gttataaccg ccgtcattag tctcccagat
    68401 ttccccatta ttcgcaataa cccatccagt caatgaatcg agaaaagaaa tatcaacggc
    68461 ataatctact ccatcttcaa aaatttgttt acagaaaatc caggattcac cataatccct
    68521 tgttttttgt atttgaccgt cccgactcaa tacccaacca taaccatttt caataaaaga
    68581 acatttgata taactccgat cttcgttatg ggtgatatgc caggttttcc ccgaatcggc
    68641 tgatactgca actccacctc tacctgttac gcaagctttc ccagccgaaa gaactacccc
    68701 ataataaacg gtatcaataa aaacattttc cggattaatg gtcgcacaat tccaggtatt
    68761 cccgccatct aaggttatag caatcgtact ttccgttcct gttatgattc caaaggcagt
    68821 gtctgcaaaa gaaactgatt gaagatcgga caatcccata tatatgtcaa aatcaaacag
    68881 ggaatcggcg actctaatcc attctttacc gccattagaa gttttaaata aacaatgtaa
    68941 cccagccgcc caaccattca aactatcaac gaaatcaata ttattaaacc aggaaaattc
    69001 ccgatatacg aactgctccg accaggtttg gcctccatct ttagtattta atattactcc
    69061 tcctactggt ccgattgttt ctccgcctgc ccaaccattc aatgaatcag aaaatgataa
    69121 tgtataaatc cgataatttt caggaaaaac aaaaattcta tcccacaatt cgttttgagc
    69181 ctctattctc tgggtaaata aaacaaaata aatagtcagc attaaaatca ataataactt
    69241 tttcatttta cctccttttc ttttattaaa acatttgtta attcacattg ttagaataat
    69301 acggttatcg cctgttaaaa taaacatttt catattacta aaactccgaa gatatatcaa
    69361 gtctattttc tataactgga attctgtctt gtaataaaaa aggataaaat caaaacggtg
    69421 agggtcgtgt agaaattaca gcctcattaa aactnnnnnn nnnnnnnnnn nnnnnnnnnn
    69481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    69541 nnnnnnnnnn nnnnaaggat aaaatcaaaa cggtgagggt cgtgtagaaa ttacagcctc
    69601 attaaaacta atcaaatact tccccatctt cgagtgtacg taaaaagaat tattttacaa
    69661 ttagttaatc aacgactaat aattcggctg tagtattgtg ctagctatac ttatatttct
    69721 gtaccaatct tttaaaattt ggccaaaaat aataaatatc taaatatctc aacgaaaatc
    69781 caccaagtct ctccctgctt actgaatttg tacttttaaa agtcaaaggt aaaaaaatcc
    69841 tccgctaata tgatacgctt agctaacgac ggcccaaatc gctcagttat atcgagatta
    69901 tcaacttctg gtgggagatg cgtgaggata acttgctggg gctgagcgac ttcgattacc
    69961 gttttaattt gctcagggtt taaatgaccg gctatcggaa cgttcgatag attaccacat
    70021 tcgatcaaaa gcagaccaac attgcggctg gcatcaatca atatttggtc taatccaaca
    70081 tctcccgtat aaagcaaaga ttcaccatca ggtccgatga accgatacat ctgactttcg
    70141 ggagcatgat tcgatggaaa aacctgaacc tggtaactag aaaaatcatg cattcctggc
    70201 gtaatcggag aaaaataagc tggataatcc ggatcaaccg tccatttttc aaacattttg
    70261 gtgagattat tgaaatagta ggcaaaatca ggatgagcgt gaatataaag tggtgcattc
    70321 gggcgttgcg gtttataata tttgcgcgta aacagtaaag gtaaaaaatc ggcaatatga
    70381 tcgagatgaa aatggctata aaaaacgcgc ccgacttcca gtaccgaacg ctcagccttg
    70441 acaatttgcc gcagagtacc cgagccaccg tccagcagga gagtctcatc gccaatatct
    70501 aacaaatagc cggagcaatt tcgcttctca ttcggtaaaa acgagccaga gcccagaatc
    70561 gttaaatgca tacttatcct cctttatcag tctatagttt tatttccatc gctaaaattt
    70621 gaaaatatca attattgaaa acaatataaa taatttggtg gtaatcgcaa ttattttata
    70681 ttttccgcca atcaacatgg agattgaatg aataaaaata acactgggga aataatccag
    70741 gcaatgccta aaatcgaact gcacctgcat ctggaagggg ctttcccgct cgaatccctt
    70801 ttacgattaa tccaaaaata cggcagtaac gaagtgcata atatagagga attgcgacag
    70861 aaatttgttt tcaccgattt tgctcatttt atcgaaacct ggtactggaa aagtgatttt
    70921 tatcgtgagc cgtccgacat tgaagatatg gcttattcta caattgcaag tttatcccta
    70981 caaaacatta tttacgctga agtctatttt tcaccattcg attttatcaa gccgcaaatg
    71041 ccttttccgg tcgtaactga agccgtaatt tccggtgtac ggcgagctga atccgaatat
    71101 ccgattcgga ttgggttaat tgccgacatt gttcgtaatc atggtcacga aactgctctg
    71161 gcgagattgg ataaaatcac gcaataccaa aatgaggttc tcggcatcgg actcggcggc
    71221 agtgagaagg aatttccggc taaattgttc gctgannnnn nnnnnnnnnn nnnnnnnnnn
    71281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    71341 nnnnnnnnnn nnnnngttcg gcgcttattt gattatggct tactgataac gattaactca
    71401 gatgatccaa ctatgtttgg ttcaaaccta accgatgagt ttaattttat cgcgaatgaa
    71461 tataatttta ccttacctga aattaagaag ctgactcaaa atgcgattca agctagtttc
    71521 gcggaagata gttttaaaca gcaacttacc ctaaaaaata atgaatattg gaattcgatt
    71581 ggcgtaaagg aataaccttt ccgggttgcg ataacattta attattttcc ttcgctaaaa
    71641 aaataatcac gaaattttat cttgcaattg aaaatgctat ccaataattt tcagccgttc
    71701 tttttaattg gattgaaaat gccgatttaa aggcgtttta agcagagaag cacaacaggg
    71761 aagctcgtga aaatcgggca cagccccgct actgtaatcg cggacgtttc tgccgatgcc
    71821 acggatcatt cgatctggaa ggcgcagaac aacggatgat gcgtaagtca ggatacctgg
    71881 tgtttttctt gccaatctgg agtacgcggg ttattaagga ttaggcagat gtatcattca
    71941 ttactcaaaa tctatagatt ttttccgctg tggcttttac ctattgcgct ggaagcaatg
    72001 gagttttcca gcggttttat ttgtgatcag aataatcagc ccattcccta tgtgtttatt
    72061 tataataaaa ctaaagacag ttttattgtc agcgatgaaa acggcatatt catccttcct
    72121 gccagctatc aagccggcga ttccctgctt ttcacgcgta tcggctacgg cgagcaggaa
    72181 atcgtgctgg gaaatttata tgaaccggta aaaatcattc tccctttcac tccagtaaat
    72241 ctggattcgg taaccgttgc cggacaaatt tttccacgtc agttgtccgg aattaacttc
    72301 agtcgagttg aacgcactga agacctcgga accgtcgagc atcgccgctt atggacaaca
    72361 attcccgggt tatatcttaa atcctatggg ggaccgactg gcataagcac attaagcctg
    72421 gacggttcgc cgtcaactca taccagagtg gtaatcgccg gttttgattt aaccagcgct
    72481 cagaacggtc agatggatat ctcacaactg ccaccgacct ttatatcgag cattaattac
    72541 tctcccgaac aagccggcaa taccagcgct gaaaattcag aaggtttgtt aaatatcgaa
    72601 cccaatatgt cgcctactgg ttttacttgc tcagccggca gctacggcaa attaaatctg
    72661 agcgccaatc tcgacattcg caaaaccgta tggaattcca atatattatt aggtcacaac
    72721 tattaccgcg gcgattatga agttacctgg cgtgatcaaa atttcaagcg ttcaaacaat
    72781 tcgttacagc aaaactattt cagctggcag tctggatacg ttttccgatc gaaggcattt
    72841 gcgcgcctga tagcttttta ctcagaacag gagcgcggag ttgccggtca ggtctggaat
    72901 ccgtcgccag atgcctttcg gcaagacgcg ctggggttta tcggtttgaa aattggctgg
    72961 gcagttaagg ccggacattc ttacattcag actttacatc gtcattcgga cgaacgctat
    73021 gtcaatccga ctattgcaat aaacagccgg cacgtcgtta acaccgatca aatcatttac
    73081 catgccaatc ggtcaatcct caattttaac ctcgatttta cttccgacct taaatccgac
    73141 cgattgcaga gtaaacaagc tggtcatcac tcgcgtatta ctttggcaaa tattctagct
    73201 ttgccctgga atttccaccc taactatgaa ctgcgtccct gggttaaatt cgagcattct
    73261 ccggatttat atcaaaaagc aacttgggga ggcagaattg catatcgcgg ttctggaata
    73321 ctcaatacca tctccggcag ttatggccaa tattacgcct atccgacatt caatgattta
    73381 tactggcaac cgggtggtaa tcccaatctg aaaccggatg aaacaaccaa atatgaaatc
    73441 gatctcattc tccaaatcct tccggagctt aaacttgttt taaatagtta ttataaaaag
    73501 gataaaaacc tgattcaatg gacgccggtt caatcctatt ggcaaccttc gaatatttcc
    73561 caagccgagc gaaaaggcat caaagtcatc ctcaactggt cattacccaa tttaccagtt
    73621 gatggctttt tgcaaggcgt tgtaacccat tcttgcaatt tgaccaaagg caacaattac
    73681 caaaaacctt tgcgttatac tccgaaggaa tcttacgccg ctggtctaaa ttttaatttc
    73741 aaacatattg ttttccatag cacaatcgaa tacgttggcg ccagaattgc gatgtacaac
    73801 tggccagaag acataatcct cgccgaatat ctcgttctga atgccaattt agcttataaa
    73861 gttaatacga caatcggacg tttcaccatc gtcgccggat gcgacaacat taccaacaaa
    73921 tcattcgaaa gtatgcaagg ctatccagaa ccaggacggg cttttaccgg gacaatcgag
    73981 tataacccaa aataaaaaga gaggactaaa aaatgaaaca gaaactgacc ttgttagcca
    74041 ttgtaacgat ttttcaaatc tcactggcgc agaacttcac ttacattcta tgcgaaggaa
    74101 attttaatac gattaatgcc tcgctctggc aattaaccgg cacgagtgag ctaattggtc
    74161 ctgtccattg gaactttgcc gataatccgc tcggcgacgt cggccaaaac ctaaaagttt
    74221 atcaggaaaa gctcttcatc gtcatgaata attccaactc gttggaagtt atgagtttat
    74281 cgaatggtgc aacttacgag cggacgatcg atttgccctt tgccggaccg cgtgatatcg
    74341 ccttcgtgaa caatcacgct tacatcacct gctggtattt acctggcata ctctcgctgg
    74401 atttaaatac ctggcaaatt gacgatacga tttttatcaa tggcttacca gataatatca
    74461 ttgcagatga tggaaaatta tatacctcaa ttgcgatgaa taccgactgg tcaccggctg
    74521 ataaagtgct tgctctggga ttgatctccg gcgaatttac acctgtggat accttcactg
    74581 tcatcaccgg tccgaatcag cttttgttat acgaaaataa gctctatgtc ctcagtaatt
    74641 actatgacga tgcctggaat acctatacgg gaacttcctg cattaatctc gaaacaaatc
    74701 aagtgaccaa acgggatcag gggctatcgt tcaatttttg tgacgacctt gctctaataa
    74761 acgataaaat atatcgcagt tataacggcg gtgtggcgcc gctaaccgat tcgcttacga
    74821 ttgatataaa taaaatcatt ggcaattatg ctggtatcta ttcaatggct ttcgacggaa
    74881 aaaatatttt catcggactg agcgattatt ctgcaccgga tcaggtggta attctcgata
    74941 cgctcggtaa cgaaattaat actctgaatg tgggcgtctg ccccggttct tttgcttttt
    75001 atcagcaacc aaatgccgaa atatttactg atgaaaaaaa tggtatttta tctgattttt
    75061 tact
//
LOCUS       DAOSMP010000005        69502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_421_length_69502_cov_19.788717, whole genome shotgun sequence.
ACCESSION   DAOSMP010000005 DAOSMP010000000
VERSION     DAOSMP010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 69502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 69502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69502
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_421_length_69502_cov_19.788717"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PKX09_01350"
     CDS             complement(<1..723)
                     /locus_tag="PKX09_01350"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOU16508.1"
                     /translation="MNYYNYNQSRYKSGYIRPRFSRGAVGNIIIANVVIYLIMVIFQA
                     QNFFAFNFGLVPYAVLKKGYIWQFFTYMFIHGGFGHIFWNMFVLWMFGMEIENYWGKR
                     EFYKFYLITGVGSGLITMLFSAHSDIPVVGASGAIYGVLVAFAMLFPERYIYFYFLIP
                     IKAKYFAIIMVVITFFSTLSPGTGNISHLTHLGGLVIGYLYLARYKLLSGWHRRTGSA
                     NWHWPRPKIKNPFRNLHINFRPK"
     gene            complement(727..1428)
                     /gene="recO"
                     /locus_tag="PKX09_01355"
     CDS             complement(727..1428)
                     /gene="recO"
                     /locus_tag="PKX09_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOU16509.1"
                     /translation="MAIIRTRAFVIKTIPFKDTSLIARLFTESNGKVAVLAKGARNLK
                     SPFRGYLEPLSLLEVQFYYKPTRDIQTLSQAETIRSFLSNSTEIEPTIMATAILECIE
                     KFIREYHEDVPIFKLVLDTLNYIEAQNDNSQLAFVYFILNLTTLMGYEIGLNSSRNSD
                     WSNLPFSMEELEILKKIEKADIRTGLPLCNLNLPIMAIAKTLVNYLAMQTDLPVRLKS
                     FELLSQIIQSADRKD"
     gene            complement(1641..3086)
                     /gene="proS"
                     /locus_tag="PKX09_01360"
     CDS             complement(1641..3086)
                     /gene="proS"
                     /locus_tag="PKX09_01360"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOU16510.1"
                     /translation="MSISVTKRSEDYAKWYTDVVLKAEMADYGPVKGTMVIRPYGYAI
                     WENIQSIADRMFKETGHVNAYFPMFIPESYMKREAEHVEGFAPECAIVTIGGGKPLEE
                     PLYVRPTSETIIWASYKKWIRSYRDLPILINQWANIVRWEMRTRLFLRTTEFLWQEGH
                     TAHATPEEAEEEARRILEIYRQIAEDYLAMPVLVGRKTEAQKFAGAVHTYAIEAMMGD
                     KKALQAGTSHNLGQNFAKAFDVTFLNKSNQLEYVYATSWGISTRLIGGLIMTHGDDKG
                     LILPPRVAPYQVVIVPIWKSEEEKCAINEFVANLTTRLKAEQIRFRYDNSEQETPGWK
                     FNYYEMKGVPIRLEVGPKDVAKNQVVLVRRDSTEMEPIGRKQPIPVDNAPTVIKNLLE
                     EIQTNLFKRAQAFRENNTFQAENYDQFKELIEAGGFIRVFWAGNSADEARIQEETKAT
                     IRVIPFEQKEKGTCFYTGKTTDRIVIFARAY"
     gene            complement(3088..3774)
                     /locus_tag="PKX09_01365"
     CDS             complement(3088..3774)
                     /locus_tag="PKX09_01365"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HOU16511.1"
                     /translation="MFDEFLKIWRKKSLTAETVDQALDMLAKTREMFVESMWSLRESD
                     TGELRLDIYQHDKTINKLVRDIRKKLMTHLMISDKSEVISGLTLAIVVNSIERIGDYT
                     KNIADLAILHKKTLHAAEFEETVVSIEKRVKDFFDWTIKAFPESNVELARQVVHQYKD
                     VSRDTTILINTLVKGECEFNISDAVTLALYIRFLKRVGAHLYNICTSIVNPFHRIGFK
                     EKNNEINQGN"
     gene            complement(3778..4872)
                     /locus_tag="PKX09_01370"
     CDS             complement(3778..4872)
                     /locus_tag="PKX09_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOU16512.1"
                     /translation="MNFRDRKYWVGFAKALAIITLIYLFLLSVTLIGDTFKMMGQGFA
                     QKIMNGVSNPFVGLFAGMLCTAIVQSSSLTTSLVVSMVAGNVLTINLAIPLIMGANIG
                     TTITNVLVALGHIGKKDEFERAFAVATMHDFFNVLTVIIVFPFQYFFNILGIAAGSVA
                     TAFENIGALKFINPISKILEPVSQFIIDLFPNYPWINIVLALVILFFALTFLVKVIKS
                     LVLAKVELFFDQYIFKNATRGFLLGLLLTAIVQSSSITTSLTVPLAAAGVLSQKKVFP
                     YAVGANIGTTITAFLAAIATNSVDAITIAFAHTLFNVIGACIFLPLQKIPIELARLLA
                     SLTRKNRLYAIAYVILLFFIIPLIIIFITR"
     gene            5191..6537
                     /locus_tag="PKX09_01375"
     CDS             5191..6537
                     /locus_tag="PKX09_01375"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOU16513.1"
                     /translation="MKSSNIFDVILIGAGPAGLFAAETLAGKSLSVLVLDRGDEPLKR
                     HDVNFGIGGAGAFSDGKLNLTSRIGGEPESFGRKADGVEELIQQIDEIFTSLGVTGGY
                     SGENGEAYQTLLRAAHAAGVEFIFAKQRHIGTDRLQDIIQRFYERLIEKGIQFCPNTS
                     VDKISREGEFFKLTCKEEVYFARAVIAAPGRAGAYWLREQAHNLGIHTMYGPIDVGVR
                     VEFPDDIYRPIAAVMYDAKFRLYSKSYDDLVRTFCTNPSGYIVTEKYDDFVLVNGHAK
                     WNSKTNNTNFALLSRVVLTDPVEDTTKYGRDIARLATTIGGGKPIIQRLTDFVSGRRS
                     NWERISRSATVPTLKDVTPGDIAMAFPHRIVTNLIEGLTVLNKIISGLMSNNTLLYAP
                     EIKFYDTKYKVTPYLETTLENFFVAGDASGHSRGIVYSAVTGIFAAQGVLKNLGKD"
     gene            6596..7072
                     /locus_tag="PKX09_01380"
     CDS             6596..7072
                     /locus_tag="PKX09_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOU16514.1"
                     /translation="MKSPENKDKFMQEGLEFIKQIVNRRGSRDTKLLAICRFLNDKFI
                     GFDWVGFYFSDNLKKELILGPFIGDPTEHRNIPFGRGVCGRAAVSGTTVNVADVTAES
                     NYLACSLEVRSEIVIPIFKSGQFVAELDIDSHQSDRFGNSERQFLEQVGQIISVIF"
     gene            7179..8768
                     /locus_tag="PKX09_01385"
     CDS             7179..8768
                     /locus_tag="PKX09_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOU16515.1"
                     /translation="MRKATIPVIIIVVVLGFLFISPFGKSIIASGSDLYQKLNIFQDI
                     IRIINDSYVEEPDWNKVMEGAYRGMLEELDPHSVYIEPKQLDDLNEQFSGKFEGIGIE
                     FDILDGYITVISPVAGAPAERAGLEVGDKIVKIDGESAYRISRDDVQKKLRGPKGSEV
                     TVTVRRLGQADFDVKIIRDEIPLYSVSAAFMIDDSTGYIFLNRFSGTTSDEVEKALNR
                     LSAEGMQRLVFDLRNNSGGYLEQAVEIADKFIAGRQKIVYTRGRISSANEEFYSSKTE
                     PYEKIPLVILINRGSASASEIVAGAVQDLDRGLIVGETSFGKGLVQRQYPLRDGSAIR
                     VTVARYYTPSGRLIQRPYDGAIDDYYDTFSAENRDSLLAVKDSVEKRPEFSTIGGRTV
                     YGGGGITPDYSVKYDQALNNETYKLLRHPSRVIFEYASDFVNKNRDLAADRKNFYKNF
                     EVSNKDFEAFKKMAVEKIGDLNIKQVEKDAEYLKILIKAEIAHGYWGYDEYYKIVRLS
                     DNQVTSAVDFLNEARKMLQIQ"
     gene            8869..9492
                     /locus_tag="PKX09_01390"
     CDS             8869..9492
                     /locus_tag="PKX09_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOU16516.1"
                     /translation="MVELFLRGGIFMWPILALAIFGIMVVIERAYTLSKASRVTKRFL
                     PKVLNTLKEEDGVRKAIELCENTPGPIAEIFHAGLSKVGYGMEAVEKAIQSAGTVEMA
                     FLDKGMIWLATVITLAPLLGFMGTVSGMINAFDAIAKVNDMSPAIVAGGISEALLTTL
                     FGLIVAAPLQLCYNLFVARVDSIVVEMEESTMNLLQTLDVINKENCK"
     gene            9495..9893
                     /locus_tag="PKX09_01395"
     CDS             9495..9893
                     /locus_tag="PKX09_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOU16517.1"
                     /translation="MALLKKKKTRKVDIPSASQADIAFLLLLFFICTTTVDVDKGIGL
                     ILPPEGDSPIEISKKNITTIVIDPQGNVLIDNKEVEINQIRDIAERLQIENPKMIFSV
                     KAHPRCRYDKYVAVLDQLKMAKATRISIAE"
     gene            9905..10312
                     /locus_tag="PKX09_01400"
     CDS             9905..10312
                     /locus_tag="PKX09_01400"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOU16518.1"
                     /translation="MAFQHKKNISTDIPTASMPDIIFTLTLFFMATTVMRQYSGLPVV
                     LPKAKQIEKLQSKIHVTELWVSKEELISIDGKLVNLQDIRNIMYNKRVRDPQLIVSIR
                     ADERVTMDLMSKIHKELRLADALKVNYSTKTAS"
     gene            10323..10994
                     /locus_tag="PKX09_01405"
     CDS             10323..10994
                     /locus_tag="PKX09_01405"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOU16519.1"
                     /translation="MILRKKPEASLKLRQPLMYRVGLVVALVLITGIMIAFPKFEERE
                     VVQRKVDIILEVTDIPQIEQKIEAAKPPSRPSIPIESENEDLSEDITIDQTTDFDSYT
                     AWEAPPPPPEQNNDEGEGPRVRFIPYDEPPEPIGGFAEIQKNIVYPEIAQEAGIEGTV
                     VVQAYVNEYGVVTECTILKGMPNTGLEEAAIAAIKKTRFKPAKQRDRNVGVYISIPVI
                     FKLKS"
     gene            11256..11936
                     /locus_tag="PKX09_01410"
     CDS             11256..11936
                     /locus_tag="PKX09_01410"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU16520.1"
                     /translation="MNLSKPTENRPIYAKTWQNVAAVERYALSRYRHYDQRLISWREK
                     RIVNHLLKQIKNGNHILLDIPSGYGRFTPLFRQHGFKVINGDLNLYALLYQRQRFPDS
                     NLMFVTDGNCLPLPDNSAGVVFNFRLLQHFQTSDQRIALLRELARVTNCYLIVSVYLE
                     SNFHRLAQLVSRRKRRMTMISRTQWDTELRSCGLKVLRISRPLRFLHAQNIFLIEKSQ
                     SCSAPIDK"
     gene            11964..14003
                     /locus_tag="PKX09_01415"
     CDS             11964..14003
                     /locus_tag="PKX09_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOU16521.1"
                     /translation="MQKIISFILTCTVLAIAAEPLDLLTVFTEPYLAGSVAANVTWSP
                     NDSLIAFLWNDQGGEFRNLWIANPQTGAKEQLTDFNPTTDVNMPSIGALGWLNNRCLF
                     FEYQGDIWEASLLPGYPLKRIEGLTNCKPPIALAPNRRYLTYNRGGYLRLFDFITKEE
                     QTIIPDKTYQRPPARQVCAYQTTYCWSNDSKKIVVYLESEADENTVHFKVFTVPKSST
                     DDVVIKEHPSDNSIVRNFIWSPDNQSIVIESVTSNLRERYILLVDLTKDRLDTLYREI
                     SNNWIADFGYQLYWIESEAKILFGMEYNSYNHLFLLDPTNGHYSALTRGKWDVKDYAV
                     DATGQLVYFTATKDNPEQLQLYSAQIKTGEITDISYRGGNYSFQLSNNSRWIAEVFSN
                     YSTPPDLYWIEASPQSKMNQITDRPDSPLEKPLRKIPKSGTIQNAITGKPVNYRVWLP
                     DDFRVNHKYPIIVVLNGADCVAPLTEAWRPECYFKQWLSDKGYIVVAVQYTDLRTAIE
                     PADSESFSDPLKIQLADIQAIVKEIGKLQYVDLSRSGICGWGYGGYLATMAMFETDNI
                     FRAGVAILNDFSWSQSSSIYLNYMANLLANDQVFRKIDPHNFCYNLRGRLLVIQSASS
                     TLPQLVDAQRLIPELLSIRRPVDFYLYPWENNPIMRSANQYDMFIKTARFFQLNL"
     gene            complement(14027..14350)
                     /locus_tag="PKX09_01420"
     CDS             complement(14027..14350)
                     /locus_tag="PKX09_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOU16522.1"
                     /translation="MNSTKPVAQIKIELPESKAEGNYSNFILISHSNAEFVLDFARIM
                     PGSPKAVVQSRIILTPIHAKTLLFTLQDNLAKFEAKFGEIKLPDKESFEPFGFPNSEE
                     SKLPN"
     gene            complement(14380..14796)
                     /locus_tag="PKX09_01425"
     CDS             complement(14380..14796)
                     /locus_tag="PKX09_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16523.1"
                     /translation="MEDLVCPECNAPLKEEDLKQSLVCPNCKTNLRDSKYLDFLELLV
                     YYDIIDDIDFFDMSLYGKEMLEHEREDFDEEEIDPSKFEKKKEVWDEFEDDLELNENL
                     TEEIESSDDAWDLSDTDIPFEDEWDEDNEDREEDEY"
     gene            complement(14849..15208)
                     /locus_tag="PKX09_01430"
     CDS             complement(14849..15208)
                     /locus_tag="PKX09_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16524.1"
                     /translation="MLVPQAFFTLIQEINPDLLIPLISSRINKEGFPTDPEVLALARI
                     NLEFAIDLNVVPTVTTSFIVSRCRAPIKVGFQSELAEKLFNLIINYKTDRLVEKSYNL
                     IWDLLHPQIQKVNKNLT"
     gene            complement(15708..16019)
                     /locus_tag="PKX09_01435"
     CDS             complement(15708..16019)
                     /locus_tag="PKX09_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOU16525.1"
                     /translation="MSRAREAFAQITENKTYPIPINGVLDLHTFSPKEIKDLVPEYLE
                     ACRAKDLLDVRIIHGKGIGVLRETVQAILRKLPYVEKFQLADESGGGWGATIVRLKGK
                     E"
     gene            complement(16026..16655)
                     /locus_tag="PKX09_01440"
                     /pseudo
     CDS             complement(16026..16655)
                     /locus_tag="PKX09_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
     gene            complement(16738..16875)
                     /locus_tag="PKX09_01445"
     CDS             complement(16738..16875)
                     /locus_tag="PKX09_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16526.1"
                     /translation="MKGIRILLNKMIFASYLGNILVFTGALYAQIADFSGERLTFTTK
                     Q"
     gene            17039..18091
                     /gene="carA"
                     /locus_tag="PKX09_01450"
     CDS             17039..18091
                     /gene="carA"
                     /locus_tag="PKX09_01450"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOU16527.1"
                     /translation="MNKRTALLTLEDGHVFEGFSFGWEGATAGELVFNTGMVGYPESL
                     TDPSYYGQILVMTYPLIGNYGVPEFKPDSLYFESDRIQVRGLIVADYSADYCHWNAHQ
                     SLGDWLKSQKIPAITGIDTRTLTQILREEGTMLAKITFDNDIDYYDPNRYNVVAEVTC
                     TEPRTYGQGKKRIALLDCGCKRSIISQLSQHRVEIMRLPWDSDLTKYDFNGLCISNGP
                     GDPRNCEASVASVRLALENKIPTLGICLGNQILALAIGAKTYKLKYGHRGQNQPVMDQ
                     TSGCCYITSQNHGFAVDDKSLPEDWRIWFSNLNDRTNEGLIHRSGRYMSVQFHPEAAT
                     GPLDTMFVFDRFLDIL"
     gene            18240..21419
                     /gene="carB"
                     /locus_tag="PKX09_01455"
     CDS             18240..21419
                     /gene="carB"
                     /locus_tag="PKX09_01455"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HOU16528.1"
                     /translation="MVKKVLILGSAALKIGEAGEFDYSGSQAIKALKEEGIHTILVNP
                     NIATIQTSENLADEIYFLPVNAEFVEKVIKKTRPDGILLSFGGQTALNTGLELHRRGI
                     LQKYDVAILGTPIQAIKDTEDRQLFIQRLQEIEVKTARSQIATTLDEALQAAEALAFP
                     VIIRIAYALGGLGSGVCRNIEEFRDKAEKALTYAPQILIEEYLEGWKEIEYEVICDSF
                     NNCLTICNMENVDPMGIHTGESIVVAPCQTLSNSESHKLRSIAIKVVRHLGIVGECNI
                     QFALDPRSEDYRVIEVNARLSRSSALASKATGYPIAFIAAKLALGYGLHQLPNKVTRT
                     TTAFFEPALDYIVVKIPRWDLKKFRNVSHRIGSGMKSVGEVMAIGRTFEEALQKGLRM
                     LETGANGIVGNNGVIFENLERELSEPTDERIFAVAEALQQGYDVAKIAKLTNINPWFI
                     YKIRNIVAMNTHLSQYRNQELPTDTLLAAKRLGFSDKQIALAIGKSDAEVHQLRLKAN
                     IRPFIRQIDTLAAEFPAYTNYLYLTYNAIRDDISFTDSNVVMILGSGAYRIGSSVEFD
                     WSCVNCGQTLRRLGYNTIMLNYNPETVSTDYDESDFLIFDEISFENVVEIYAKFSALG
                     VIVSMGGQIPNNLALKLHHANVRILGTSPESIDRAENRRKFSDLLDKLNIDQPIWKEI
                     TSVELAKEFAHEYGYPVLIRPSYVLSGAAMAVASNDEELVQYLQRAVKISPEYPTVIS
                     KFLENAKEIECDAVARNGEIITYAISEHVENAGVHSGDATLVLPPQRTYLETIRRIRI
                     ITSKIAQNLKINGPFNIQFIAKQNEVKVIECNLRASRSFPFVSKVLKRNFIDIATQVI
                     MHVPLEPIPVSLFELNHVGVKAPQFSFTRLQGADPTLGVEMASTGEVACLGDDFDEAF
                     LKALISVGYHFPPRAALISSGPIESKAELLESIRTLQNMNVKLYATGGTAKFLEANKV
                     SVETLFWPLEKKEPNVITYLKNGKIDLVINIPKSYQERELTNGYLIRRTAADFNVMLI
                     TNRQIAMRFIEAISRLKMDDLKIRSWGEY"
     gene            21432..22259
                     /gene="mutM"
                     /locus_tag="PKX09_01460"
     CDS             21432..22259
                     /gene="mutM"
                     /locus_tag="PKX09_01460"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006341665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HOU16529.1"
                     /translation="MPELPEVQTIINDLIAANLVGQKVLEVEIHWQKIIAEPSAEKFT
                     TEIQNREITKITRRGKFIVIELKPVKFLLVHLRMSGKFSLTAPDYPISKHEHLIFTLN
                     DGRQLRYHDTRKFGRFWLVNQPEKVIGKLGVEPLSADFTVEILTENLRKHHRQIKPFL
                     LDQTIIAGLGNIYVDEALWEACLHPLRMADSLNDQEIASLHKAIPKVLRQGLANQGTS
                     LGNGKGNFQSVGKRARNQEALKIFRRNGQACPRCGAIIERLKVGQRSTHICPHCQLI"
     gene            22448..23281
                     /locus_tag="PKX09_01465"
     CDS             22448..23281
                     /locus_tag="PKX09_01465"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOU16530.1"
                     /translation="MAVSTFLSPEAGWGVPLTQPISQIVRALSLDHFPNSWLNIGLWL
                     VLITGMLSAVFLKGMRDFAPKFLHCLLAAVFLLIFYDKAFNQRFVISIQEGQEVNFAQ
                     FLKKSKPEYNVPLRLLDFDIKIHPDGKTAASYASRLVVNQTDTAILAVNHPLSIGKYR
                     LYQSAFRKDYLFEVICDGDTIISPFNGACNFNQQEIFLRGVDTLAQALTVEIDRNEYK
                     IPPGEVCNVAGRQIKINPLSPKYTSIIEVAEVTGLKFLAFFSLMYLGVLTYIILWKKI
                     R"
     gene            23297..24061
                     /gene="ccsA"
                     /locus_tag="PKX09_01470"
     CDS             23297..24061
                     /gene="ccsA"
                     /locus_tag="PKX09_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOU16531.1"
                     /translation="MEQPLLIGALILYTIAFVGLITKVKDQICSVVIWLAILTHLAAL
                     IWRSLAAGHPPFTSLYETILLLSFLLALRLVIWRRQIESRYRWIVLLIIMGMIIGALI
                     IPLSYKTIKPLSPALNSCWMYLHVPAYFFGYMALVLAVIYALIFLVQNKNESSSDGMT
                     LLRKMDNEVQIAFLFLNIGLITGAFWGYQSWGNYWSWDVKEVWALLNILVLAFYFHLL
                     KPRGVKKAVIVIIAFLTVIFTYFGVSFLLKGLHSYL"
     gene            24196..24642
                     /locus_tag="PKX09_01475"
     CDS             24196..24642
                     /locus_tag="PKX09_01475"
                     /inference="COORDINATES: protein motif:HMM:NF025049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOU16532.1"
                     /translation="MERSQFENILDFAIEREWESVRFYHRLLQEAPSLAMMEAIRGIE
                     LMERQHINTLEQFRKAGFKSQAIPSVDNIQLGEYYVPAVENQNYRNMLMTAIQREELS
                     HKLYKALAETSRDPEVADLFEKLASEETQHKSYFESLYDSEVLKEN"
     gene            24811..26175
                     /locus_tag="PKX09_01480"
     CDS             24811..26175
                     /locus_tag="PKX09_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOU16533.1"
                     /translation="MGTMIESVTNDTTQRTAKSPEEIINIFNAKKVDDDWSFAGYKPS
                     DTGKWTHAYHRYPAKFIPQLVEKLIAEYISHKEAHINDPFIGCGTTIVTAISKGFKAS
                     GTDINKIAYLITKVKSTPIEPVYLNKKINQILFKLKSLNGTRRTLFNDKVEPLIPQKH
                     IDRINYWFIEENKNELGKILRVIYNEEDETIRNFFLVAFSHILKNCSIWLQGSTKPTR
                     DLKKNPVKPYDAFRRHLMKMQSGNNAFYKIVPAIVRENLSAYLNIENGDARKQPTSDE
                     TVDLVVTSSPYVTSYEYADLHQLSTIWFDLANDLTEYKKEFIGTSYKKYENKRLRSSI
                     AMDIVDKMLPKYKKIAKEIEAFFIDMEEVFDESFRILKHGGRCCYVIGDTKLKGVDIL
                     NAEVFAESIQYSGFKLDRIIKREIPSKILPQKRDEKTGRFANNYEANSEAYPVEYIVI
                     GLKE"
     gene            26181..26867
                     /locus_tag="PKX09_01485"
     CDS             26181..26867
                     /locus_tag="PKX09_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaWI family type II restriction enzyme"
                     /protein_id="HOU16534.1"
                     /translation="MKFEDLKKFYLEKKGQFGAETYKHISELLKEAKEIHKRDWLKHP
                     TPNGDHEQSWRAFKGKNLEKLVQYIITDEVEEIGLKVVNGNQLERSTNLSKELSQVKR
                     NLAIDYGEFGLHLPDVDIIIYNPQNHKVIAVISSKVTLRERIAQTGYWKLKLFQDDTT
                     KHIKVYFITPDEDGTLTSKKPAKKGRAIVEVDLDGSYVLTEEEIEESNKVKLFEHFID
                     DLKRLINNEK"
     gene            26857..27546
                     /locus_tag="PKX09_01490"
     CDS             26857..27546
                     /locus_tag="PKX09_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOU16535.1"
                     /translation="MKNNQPKFETTTLWDFPTQNYGDTPHGDNKYHGVTPAFVIWNLV
                     QRYTTEGDLIIDPMCGSGTTIDVCKEEKRKVIGFDIVPYRNDIKQADARNLPLDDEIA
                     DFIFIDSPYSDNIDYNKHPDNIGNISCEKEEFFIEIEKVAREAHRILKPNKYMAWLIG
                     DHWRKKSGFIPVGFKIYTILEKYFKPIDMIAVSRHNQTSNTPLWHERAKQHNFYLRGF
                     KYLIIMQRMSK"
     gene            27758..28879
                     /locus_tag="PKX09_01495"
     CDS             27758..28879
                     /locus_tag="PKX09_01495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU16536.1"
                     /translation="MAVNIVEVATPRQLKQFIKFPLKLYRNNPYYIPPLNFDERNTLD
                     PRRNPACEFCDTRYWLAYKNGKLAGRIAGIYNKRFVEICGKKYLRFGWVDFIDDEEVV
                     VALFKALEDWGKELGVEAIHGPLGFTDLDREGMLIEGFQEIGTMATIYNYPYYPKYLE
                     QLGYQKDIDWVEYDITVPKVMPQKLVNVSQIVKQRLGLHTLQIKKIKDVLPYAHQIFE
                     VINATYAVLYGFVPLTEKQIDTYVKQYISLMNPDFIAIVLDKNNQIVGIGLTMPSLAR
                     ALQKARGKLFPFGFVHLLWALRHAKVADLLIVGVLPEYQGQGVPALMIAEIGMKLIRK
                     GIQRAESNPELETNIKVQSQWKYFDLRQHKRRRCYIKHV"
     gene            28910..29761
                     /locus_tag="PKX09_01500"
     CDS             28910..29761
                     /locus_tag="PKX09_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOU16537.1"
                     /translation="MFAKKTDIGNQPVRSLQNVRHIILDLDGTIYNGHTLFPFIKDFL
                     DDLKNLQVDYTFLTNNSSLSVTAYIRKLRRMGLPVKPTQVYTSAQATIDYLRRRHPAI
                     KRIYLVGTQSLRREFIDSGFTVVGDDPTNEPDAVIVGFDTSLTFQKLCGAAYWIKTGK
                     LFIATHPDLTCPTDQDTVLVDCGAVCAALKAATGKKPSAVLGKPDPSMIKGIMQRFGL
                     KKDEVAIVGDRLYTDIKMARQAGIASVLVLSGETTQADLQESPILPDYVLENVGELSK
                     ILRQSRK"
     gene            complement(29906..29982)
                     /locus_tag="PKX09_01505"
     tRNA            complement(29906..29982)
                     /locus_tag="PKX09_01505"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29946..29948),aa:Asp,seq:gtc)
     gene            30494..31597
                     /gene="ychF"
                     /locus_tag="PKX09_01510"
     CDS             30494..31597
                     /gene="ychF"
                     /locus_tag="PKX09_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOU16538.1"
                     /translation="MGFKCGIIGLPNVGKSTLFNALTHAGVAMENYPFCTINPHIGVV
                     NVPDKRLDLLAQIYKPDKIIPTTLQFVDIAGLVRGASQGEGLGNQFLSHIQAVDAIIH
                     VVRCFENPNVAHIDEKLDPQRDIEIVETEILLKDLEIVENRREKLIARAKSGDEQTQK
                     QLKILDDIRSLLIKGQSAKQYHSHPEEEYFIRDLALLSSKPVIILGNISEDEAISGNK
                     SAITEAFEKFALQTGNLFLTVSANLELELSELPPDDRKLLMNEWKITEPGLDRLIRAG
                     YELLRLITFFTMESKIVQAWTIPVNTPAVKAAAEIHTSFEDRFIKAEVRHWKDIESLR
                     SENLLREQGLVHIEGKSYLVHDGDVITFKLAAG"
     gene            31566..32294
                     /locus_tag="PKX09_01515"
     CDS             31566..32294
                     /locus_tag="PKX09_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU16539.1"
                     /translation="MSLPLNSPPANMASLVSQFDKLNSQYNLIADNQKILIAVSGGID
                     SLVLSRLLFELRLAEFKNLELLAAYVRQKSVALINEALKEIADLLADWQIPFSILDSE
                     LPESSQINCYACARERRKRLFQFAIMHDCNAIAFGHNLDDYLETGLMNLVFKGNLESL
                     QPVQIQLGGRIRIVRPLLNWPKKHIRTSGRAYGIKSIAPVCPMASFNKRQDINAMITS
                     FSRLNRDFRSNLRRAINHWNQLSI"
     gene            complement(32430..33023)
                     /gene="nadD"
                     /locus_tag="PKX09_01520"
     CDS             complement(32430..33023)
                     /gene="nadD"
                     /locus_tag="PKX09_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOU16540.1"
                     /translation="MKVGLFGGTFDPPHNGHLEIARAAIRAFSLNKLIFIPANISPTK
                     TSEIHTSPVHRLMMLKLALQNEPSCEISTIEIERGGISYSVDTIRAIKHQMRLEREDL
                     FFVVGADSLIEFHKWRQPEDILQEVQVVVARRPGCDLSLVKPEYLAKVLFFNAPLIPF
                     SSSDIRQIAMNGGQIDQFVPPAVAEYIRQNELYRRSA"
     gene            complement(33020..33820)
                     /gene="bamD"
                     /locus_tag="PKX09_01525"
     CDS             complement(33020..33820)
                     /gene="bamD"
                     /locus_tag="PKX09_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOU16541.1"
                     /translation="MPPKRQILKIVLISLLIIYLNNCASNKPKPVVDFEQHYRQGTEY
                     FNKKKWDKAIEYFTLVVINSPGSELADDAQFYIAECYFKRKEYLLAASEYQQLINRYA
                     YSEYVEEAFYKIALSQYKSSPRYQLDQEYSIYALQSFQDFIDTYPQSRFRADSEQKIA
                     EIRSKLARKLFESGKLYRKMQEWKAAIIYLDKVLESYYDTEWAEKARLEKAYCLIRQR
                     QFDQYELLFKEILARSTPQTDPNALEYLRTSYHKELRKIEREKRKRAD"
     gene            complement(33865..34767)
                     /locus_tag="PKX09_01530"
     CDS             complement(33865..34767)
                     /locus_tag="PKX09_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16542.1"
                     /translation="MKSKFLAATLLCAILIPIMGFGQSFFDERTTVTERDRLIYSMKY
                     AQADEDTFVFNYEKSPGLAFFLSAVVPGAGEFYAGAKYRAAAFFSVEVVSWVGYFNRK
                     NNGEKLERKYMNWANTYWDAWQWYENSLSYPEYFGLSDSGSGFGSHTIYFEYEGKEYK
                     ADVSYLDRELPGWQTYLQEGTLRPIKTRDYYENIGKYDQFASGWSDFEQIVSEVDSLN
                     IPTSPLRKKYLNQRYESNQSLKMATNFTTVIMFNHLISAFHAQIAAKNYRPPEQKDVS
                     WHVGLVTDCTYKMPIRGLSLSVAF"
     gene            complement(34778..37819)
                     /locus_tag="PKX09_01535"
     CDS             complement(34778..37819)
                     /locus_tag="PKX09_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16543.1"
                     /translation="MSERQICRNTLVIFLIMLFFFNPAGAQFGTYNHPELEWLSFETT
                     HFKIHYHQGAEWTARRTAEVAETIYGPITEFYQFEPSEKTALVIRDTDDISNGASYYY
                     DNKIEIWATPLDFPFRGNHDWLRDVITHEFTHIVSLQKAMKFGRSIPAIYIQVLDDEP
                     EKREDVVYGYPRIIASYPISGMVIPMWLAEGMAQYMFPGNSNDFWDTHRDMLLRDRVL
                     HKSLLSLSEMGSFGKRGTGNESVYNQGFAFTRYLAEHYGTETIPRLAEELSKPVRMSG
                     ETALKNVTGVAADEIYQQWQTELEQRYFKQTEIIRANEKVGEIILAEGTMQLGPEWSG
                     DSLLYFMSNKGRDYYSQTALYVYDFHQKKSRMLVPRINSRVTVAPDGQNLYYSRASKP
                     NRHGSVFFDIYRYDLKKRKEKPLTKSARVYNPTLSPDGEIIAFVTGRDGTSNVALMNL
                     DSNKIREITTFSEGVEVFTLHWSPDGRTIAFDYLIGHGRNLALVDVETGAMQILDEAA
                     YDTRNPYFSPDGKWLYFASDETGIYNIYRQSLTTGERQLLTNVTGGAFMPAVNEKGEL
                     VYAILDDRAFKIACIKNPEPIEPQLAVYLDYDQNIPPVSEIQPVSIPEPQKYHDQFAK
                     MFIMPRLMIDYGTVKPGFYCLSNEIINRFNIFAGASINRIKDRDLFLILEYHQWEPTI
                     FLEFYNISRNIFNEKSIYKGWPVEADYTFYLTEAYGGISRPFGGFNNVRFDVKWSRYR
                     TSSDETITAADIYQNGFSYDYYRGVNFQLHWQMRMMLPKVNEETNPDNGILLDTYIAR
                     NYDKFIQGFKVNSSYSTIQVDFRNNYYWRLEHEGNWYHKYPLFKNLVGTLRWHLGWIS
                     KPDVDSFFYFFAGGEPGLRGYPFYSIEGRNLSSWHYTWRLPLFREKHFQILTFNLQNA
                     FVATYIEAGNAWNQVTGFPGLKWEQFKTQPVSVLRNIVNDFKRDIGFQVRMSGFSFYS
                     YPTSISLDFVYGLDDFEVVSNSKTYQYGHEWRTYFTLLFGL"
     gene            complement(37819..40953)
                     /locus_tag="PKX09_01540"
     CDS             complement(37819..40953)
                     /locus_tag="PKX09_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU16544.1"
                     /translation="MKIYKYLITGITLIVLANKAAYGRFEPQYRIATAPSVVKNTAPA
                     AIRICALRVSFPADEDETTTGTGQFLMSAVETPCDDEFVVDPAPHNRAYFRDHIRSLA
                     NYYNRVSRGKVVIDTNNSEVFPLNDNNSYQVDHPMAYYHPFLVEDLVDLRLTELLVES
                     VQKADPEVDFAQYDVVIVFHAGVGQDFAITLDPTPYDILSAFMNKDDLKAFLAPNDSD
                     FQGIPVENGTAFVDCGLILPESENHLLYDNWEEVFGESDSPCHYQFGLNGTLAFMFGF
                     YLGLPCLFDTESGMTGIGRFGLMDQGSANLSGLVPAVPCAWERVFLGWETPVVAKNFE
                     DVVLKKVGSASDTSVWKIPINANEYFLVENRCAHFRPGVSFDSLQYRIYMENGMVELP
                     LSFPLIRDSLKAEFSPETGVMLSVPHYDYGLEGSGLLIWHIDESVINANLATNSVNGD
                     REHRGVDMEEGDGAQDLGYEADILNEDIEDGWFFDPWFAGNDGFIDLNPDYILDAEEH
                     VGFTPYTNPSTCSNNYTFTGISIDSIGPAGEVITFRIRLNDALPNFPLSLSAGADEIL
                     PLDLNPNDQVEEFLILADSIRIHTSFGEQIASLPRQTAIALDKDSLLLYGILQNNPEA
                     SSLSLSIWKFGQNGQINKTDTVSLNSQDLVSPLLPVKGGVLFATQDYADNQNYLHLYQ
                     IDTQNIESLELASQVKMLVGDDDNIFGITDDNQVIRIHLNSLSAEVVADLPNALSGTM
                     VIAFINQNESVDLIHCADSLLTLVLDIGTPNEVVLHREQEFNDNLACADLDDDGKIEI
                     ITATSSQVLAFNEELTVKNNFPIGVPNILGSDRFESELLTADLDNDGKKDVIINMSSA
                     LLAFNYKGDLLSGFPKIAYQQSQKSGILLNTPLGITYLALTSTESNPQNYNCIKAVRL
                     SSFSHDSEDWICYGGNAARQFYYPVQSQSSPIQSKSLLDNARTFCWPNPPKDNRTYIR
                     YFPNSSCNISINIYDLAGDLIADFKDSAPLIGEPNEKEWDVSNVESGVYFAVVQAKSS
                     SRTYSKVIKILVVK"
     gene            complement(41011..42234)
                     /locus_tag="PKX09_01545"
     CDS             complement(41011..42234)
                     /locus_tag="PKX09_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOU16545.1"
                     /translation="MKRIIAMLGIISLMTAGALAQGEAGAVWLLINPGARFSGMGEVG
                     AALADDAYASYWNPAGLGFLRGRELALTHVNWLPNLVSDIYYEFIGYRQFIRNVGTVG
                     GNIILLNLGEQQRTDEFGHDLGTFNSFMLAVTGSYGTALTRTSSIGVGVKMVHQHLTD
                     QGAGKELGKGYSTNFCFDIAYLKHSFLTKNLTLGINMANIGPKIAFIDVNQADPMPTN
                     MKLGLAYRLGNEYNKVTIAFDINKILVAAYPDRDIDGDEKVGGYDENGHAVPGGEYNK
                     KGQREYAHSDEWYTGIFTSWFDDWLLMYDSATDGIGSADKDGNTNDGSLAAEFEKLTY
                     NFGIEYWYSNLFALRAGCMYDKPGNITMKGKYPVPTFGAGLRWAGMGFDFGYTAGDPG
                     HPRQNTMLFSMNIKF"
     gene            complement(42382..42546)
                     /locus_tag="PKX09_01550"
     CDS             complement(42382..42546)
                     /locus_tag="PKX09_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOU16546.1"
                     /translation="MLSNELLEILVCPQCKGELEYDISNQQLICNVCRLKYAIKDDIP
                     IMLIEEAEKF"
     gene            complement(42635..43087)
                     /gene="nrdR"
                     /locus_tag="PKX09_01555"
     CDS             complement(42635..43087)
                     /gene="nrdR"
                     /locus_tag="PKX09_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOU16547.1"
                     /translation="MRCPFCSSPESKVIDSRTVQKNNAIRRRRECIDCGHRYTTYEYV
                     VEYPLQIIKNDGRREEYDRNKLLNSIKIACNKRPIPAEKLETLVIDIEKEIEETSGSE
                     VPSSQIGELVMKKLKELDEVAYIRFASVYKKFKDLDEFKEHINKFGGN"
     gene            complement(43095..44399)
                     /gene="glyA"
                     /locus_tag="PKX09_01560"
     CDS             complement(43095..44399)
                     /gene="glyA"
                     /locus_tag="PKX09_01560"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOU16548.1"
                     /translation="MEYNFLAKQDAVLLDTIKAEIERERKTLEMIASENFVSPAVLEA
                     AGSVLTNKYAEGYPGKRYYGGCAAVDQAENLARDRVKKLFGADYANVQPHSGSQANMA
                     VYFSLVNVGDTVLGMDLAHGGHLTHGSPVNFSGKLFRIISYGVKKDTGRIDFDRLAEL
                     ALEYRPKMIIAGGSAYPRFLDFAKFREIADSVGAFLVADVAHPAGLIAAGLHPDPLPY
                     CHVVTSTTHKTLRGPRGGLILMGKDWENPFGLVAPKSGRTKMMSEIIDSNVMPGIQGG
                     PLMHIIAAKAVAFGEALRPEFKAYAQQIIVNAQVLAEELLHRDYNLVSGGTDNHLILI
                     DLSNKNITGKQAENILEESGITTNKNMVPFDQKSPSVTSGIRIGTAALTTRGMCETEM
                     RTIAGLIDRVLSNPDSEDIHQKVRSEVGELCGNFPLYQEIKL"
     gene            complement(44404..44877)
                     /gene="rpiB"
                     /locus_tag="PKX09_01565"
     CDS             complement(44404..44877)
                     /gene="rpiB"
                     /locus_tag="PKX09_01565"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOU16549.1"
                     /translation="MDHGRTEEELNMVIAFGADHGGYRLKNALIEFARSEGYQVIDFG
                     VNSDDSADYPDYARAVGEAIVSGRAQFGVLICRTGAGMCISANKIHGIRAVMAYQPEI
                     ARLSRLHNHANVLCFGADFTTVESAKEILKTFLETPSSDDLRHLRRVEKIMKLES"
     gene            complement(44847..46133)
                     /locus_tag="PKX09_01570"
     CDS             complement(44847..46133)
                     /locus_tag="PKX09_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU16550.1"
                     /translation="MKRWIHFIEEALIVCLIIGLMNCTPPSPESSESVMEQVETKPTI
                     DRDKCDLYLSFAYSYYQNQNWRSAIENYKKMSSFGCEEEYAKDLFPFWGRAYQQLASE
                     NSIYLDSALYVYLAGEKYLPNDLFLKKNIAYIYHLQGKIDLEIREYEKMIEIAPEDIE
                     LYRTLVKLYISAQRYEDVIYATNGILKINPNDEQALNDRIFAYQKLGRDITTIQKEQW
                     EKNPTNIRYGLEYAAALKQQREYDKALEVYQKVAELDAKNIEAWSNIADIYFTQKNNY
                     AEALKAYDQILRNISPNDLNILQQAARCCQLLGDFPKGMEYAQKAITIAPNSPQAYKI
                     RADLYYAAADYYTSGRQITFEDKLVYKLAYDDYLKVMELGDASVKSRIDHLKEYLIPT
                     PEEWFFNRYDANGAMRTSFRPLSPEYKWITVEPKKN"
     gene            complement(46152..47375)
                     /locus_tag="PKX09_01575"
     CDS             complement(46152..47375)
                     /locus_tag="PKX09_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16551.1"
                     /translation="MKTSRRTILFLLILSLNGWSQISYYANYGFGVESPANSVRILGM
                     GRIGTAVRDSMSLNQSNPALWQGFGSTSLQGQMYTSALSIPDLDFKGRIVNFSGFSFK
                     LPVGKRAGLAIGLAPLSRMKSSVSFADSLIFQDDKFEYNSVVELSGGISELYLGSGYR
                     VSPNLAIGLRANFLFGNYVVRNHTEIDSDIDSESYYKRTIAVDGSRISFGLLLSDRSQ
                     RYNLAATVEKGNELKRWTIIENNFGPDTTIKSDPINFPTIIKVGINLPIARNLALNAD
                     MQYGIYEEDLFQNFYIFQQFKSDSRNSYAVGIGLERSPRQELKSAFLQKLYYRCGAYY
                     QTEPVYQNSGIYEYGVTAGISLPFFYNLNRIDFALTYGLKNGFLSDEIGNEKILGIHL
                     GITTGEPWFRRYKKQ"
     gene            complement(47372..48616)
                     /locus_tag="PKX09_01580"
     CDS             complement(47372..48616)
                     /locus_tag="PKX09_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16552.1"
                     /translation="MKSSILWNSSKPYFNRVWSKLHYVKIDYRRSVAAFFCLLLFGCS
                     EKIPTLPLDKNLCSFDSVSVSAEFIMDTTVFAEPNIGYSSYLVVGTDDNLTAYSLLLF
                     KELGVPLDTLPLENILTCELVLMTDNYLLNDTSTHPSLEMTVASIEGEENADWSEDST
                     NSTNFSLDDYHLTDWKTFTYSDADTLDLTTSFIANWRDSTQSHYGIVIKPAVGSAAGL
                     GIIYSGETNSSPHIIISYITDNGDTTSAVLACVGDVTITQFKKTFTAPPQPLLISAGK
                     AAYTFLKFQVEELITDKNLFIAGAYLYLHVNSELTEDYGNSHTIYITLLDSAEWESGS
                     FSPSGSSYVTSQTFSASDSILILKIPSTVQLFTSGNISNFGVALWISPSGLYPAMLGF
                     YPTEDPDPARRPRLEILTMQEE"
     gene            complement(48585..49421)
                     /locus_tag="PKX09_01585"
     CDS             complement(48585..49421)
                     /locus_tag="PKX09_01585"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOU16553.1"
                     /translation="MIKRIYFLTSEIIPFAETYQLAIFSKEIPMVFNERKYDFRLMMP
                     KYNFISERRYILREVIRLREMDLQYRGENIKASVKSAFIPNTKVQVYFLEHPYYFNNS
                     DKNLYPTECVSDEYLNSVRFGYFAMAALKTLSYLRWKPEVIICNDWQMSLIPLFIQSN
                     LVDKEYCEGIKILQIIHSKVDCSNFKVTEYQKIGLNNLDLSKSVDGRLDVVAAAMPYC
                     DHVLIINKNENLLEKYQKDPIFSEIMKQDKKKFSSYELNGESPEDWQKLADEIINIME
                     QL"
     gene            complement(49430..50332)
                     /locus_tag="PKX09_01590"
     CDS             complement(49430..50332)
                     /locus_tag="PKX09_01590"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU16554.1"
                     /translation="MQQPIKNNLKFKPSAIKLFVLALSLMVINLDLARSDSKRLSNAE
                     IAASLDRLAMDISDLKTQIANLTKTVNSLTAKDKESITKATLTTDINKLIEPLQLTMA
                     NLENRLNILSAEQANMQKRLNTVEQRTRYADSINFEILSQLVILENRLASLSNSISDY
                     NSASQTDARQATNRAITSPGITYKDRYLNALSLHQKGKFEEAIALFRKLIADDPTNEL
                     ADNAQYWIGESYYSMKQYQQAIIEFEKVRNFTNTDKDDDAQYKIGLCFKQMGDQKRAR
                     DEFEKLINLYPTSEYSERAKQLIK"
     gene            complement(50417..51667)
                     /gene="rodA"
                     /locus_tag="PKX09_01595"
     CDS             complement(50417..51667)
                     /gene="rodA"
                     /locus_tag="PKX09_01595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOU16555.1"
                     /translation="MRRSISKRINNFLNSFSNLNLAILVIVGLLCLFGMLSIYSASLN
                     MEGSFFEKIIGRQLIWLVLSGLVAATIYFLQKKLIYDSAYILYLLGFCLLLLPYFLGS
                     SVAGTARWITFGSAGFQPSEFMKILVMLAIARFLASTSSSNLDFKFVLIPTLLSLIPM
                     AVVLKQPDLGTSIIYFGMLFPMLYWAGARLFHLFIIIAPILSIITAFNFYTFFIWVII
                     VVIILYLTKEKLRVSLTVMLINLSLGFTTPFLWNSLKPYQQQRILTLFNVNADPQGTG
                     YQVLQSKIAIGSGGLFGKGIARGTQTHLKFLPEQHTDFIFSVIGEEYGFIGVLVVLLL
                     FLAFILICINIAYRQKDRFCSLCMIGIASIIGIHVVINVAMTVGLMPVTGLPLPFISY
                     GGSFLMTCFILVGLALNIGIDKQY"
     gene            complement(51648..53453)
                     /gene="mrdA"
                     /locus_tag="PKX09_01600"
     CDS             complement(51648..53453)
                     /gene="mrdA"
                     /locus_tag="PKX09_01600"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOU16556.1"
                     /translation="MYISHNSITNIRKNILTIFFVLVFLVLIGRLYYLQIIKGPSYKG
                     IAEANRVRVIPIEAPRGIIYDRNGKIIVDNKSQYDINVIPYEFSHTARTDSILAKILG
                     LDVAEINSRIKNNWRGQFVPIKIAEDVNVEIITNIEEHRLDLPGVVYSVKPVRSYPSG
                     VQMSQSLGYVREISANDLIREKYKEYKQGDLIGWKGIERQYEHILKGTRGYNFIQVDA
                     LGREIGQVVDRDNIPPIPGNEMFLAIDLDLQIYIESLTEGYKAGIVVLDADNGEILAI
                     VSKPDYDPALFAGVISNSSWNALISDPDLPMFNRATQGTYSPGSTFKLIAVLTALETK
                     AVDPDWSVRCYGQYRLGHRIFKCWQERGHGSMNMYEAIVHSCNVYFYNLIRRLDLNDW
                     ANMGKDFKFGSETGVDLYGESAGVMPDVDFLNRKYGEHGWTDGNKLNLVIGQGDILVT
                     PVQMARFCAALATRGKLVQPHVGLKYIDRGSNETIIFKAAKDSIQNISVDTWEFIEKA
                     MADVINRGTGRAARVKDVPVLGKTGTAQNPHGDAHAWFIGYMEEQNQTIATAVLVEHG
                     KSGGGIAAQIAGQIFNFIHQQKKRQSLANNAPLNF"
     gene            complement(53457..53936)
                     /gene="mreD"
                     /locus_tag="PKX09_01605"
     CDS             complement(53457..53936)
                     /gene="mreD"
                     /locus_tag="PKX09_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOU16557.1"
                     /translation="MLRKILLIIAFSLVTIIIQILLGPWMTIRAIRPDFLLILVLFIG
                     RSEGKVAGQIYGFLIGLIADALGLGSFLGLSALAKTIAGFGAGLLKKRRSHLNPILFY
                     AIEVLIIIIHFSIIYLINFKGAELSTQTIFLRYILPSTLYTGVFYFIIQYFAPFSLE"
     gene            complement(53936..54724)
                     /gene="mreC"
                     /locus_tag="PKX09_01610"
     CDS             complement(53936..54724)
                     /gene="mreC"
                     /locus_tag="PKX09_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOU16558.1"
                     /translation="MWLKIIRFIIDQYEYLLLIFLIFLSLFILAHNNSPQIRATQGEL
                     ADAFAFLHYPKVWINELSNLVEENKELKEENLRLALLNAQLKEAYLENQRLREMMNFA
                     DTSRLTILPAEVISQGTTPLYNSIMINVGSKHGVGPNMAVITNDGVVGKTVAVGDNNT
                     LVQIFLDVNFRLGVKFQISREHGIMRWRPNGLAEVREIPKTAIIYPGEKVLTSGFSEI
                     YPPNIIVGEVVEVHQSANNLFQIATVRPSVNINSIEEVFVVLSK"
     gene            complement(54730..55743)
                     /locus_tag="PKX09_01615"
     CDS             complement(54730..55743)
                     /locus_tag="PKX09_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOU16559.1"
                     /translation="MGLNLFNIFTGDIAIDLGTANTIIYMKGKGILLDEPSIVARSTH
                     DGEVLAVGADAKDMLGKTHHDIMTIRPMKDGVIADFEMTDAMIRGFIRKINLSRIARP
                     RMVICIPSGITEVEKRAVRDSADRANAREIYLVDEPVAAAIGIGIDISKPVGNIIVDI
                     GGGTTEIAVIALNGIVTKQAIRVAGDEMDEAIVQYFKTEHNLLIGERTAEYIKCSVGS
                     AMPINDTTIQVKGRSLVAGIPKTIEVSSVEIRECLKDTLDLIIQAIKQALENTPPELS
                     SDILDRGIILTGGGALLKGLDQRIRVETDLPVNVAETPLHSVVKGAGMIIEDPEKYRD
                     VLV"
     gene            complement(55760..57310)
                     /gene="purH"
                     /locus_tag="PKX09_01620"
     CDS             complement(55760..57310)
                     /gene="purH"
                     /locus_tag="PKX09_01620"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOU16560.1"
                     /translation="MSEFKIKRAIISVYDKSNIGNLAQALARKGVQIFSTGGTYRALQ
                     TAGIAVEKIEDLTGFPEILDGRVKTLHPAVFAGLLADATDESHRADLARVKVEPFQLV
                     VVNLYPFSETYHSGNKSFEEIIEMIDIGGPSMLRAASKNFKNVVILSDPGQYDEFLQR
                     LDQDELTLEYRQKLAAAVFLKTMTYDSEISNFLTGAGAEKMPEVIVQAFHKQQTLRYG
                     ENPDQSAALYAPLNNPNWQPFQQLQGKEISFNNYVDCLAAYKCYATFKTERAAVVIVK
                     HTNPCGFAIGDTTLEAYRRAVQTDPVSYFGGIVGVNREIDANLAEEFTKSFLECIIAP
                     SYSNQALEILSKRKNLRLLMPNAEALHYDLDLRSYGQGLLVQEIQAAEDDESQWKIVT
                     ESQPDPSLMPTVRMAWHVVKHLKSNAIVFCDANGTVGIGAGQTSRVDSVKIAIRKAGE
                     AGLSIKNTVMASDAYFPFRDSVDIAAEQGVIGFIQPGGSIRDQESITACNERKLFMIF
                     TGKRVFKH"
     gene            complement(57307..57927)
                     /gene="purN"
                     /locus_tag="PKX09_01625"
     CDS             complement(57307..57927)
                     /gene="purN"
                     /locus_tag="PKX09_01625"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOU16561.1"
                     /translation="MKNIAVFASGRGSNFAAILDKIQKGEIAARVVCVISDNERPPVF
                     EIANAAGIPNFCLNRKQFATPEEYANALLNVLEKYDVDLIVLAGYLKLIPSPVVRKYP
                     RAIVNIHPALLPNFGGKGFYGMKVHEAVIASGVKFSGVTIHFVDERYDTGSVICQEKV
                     AVLPDDTPESLAARVLEVEHRLYPEVVKALCEDKIKFVNGKVVWEK"
     gene            complement(58041..58871)
                     /gene="kduI"
                     /locus_tag="PKX09_01630"
     CDS             complement(58041..58871)
                     /gene="kduI"
                     /locus_tag="PKX09_01630"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HOU16562.1"
                     /translation="MEIRYLADPIRFQRMTTSELRQNMLVENLFQAGELRLVYSDADR
                     AIVGAAVPANKPLTLSGSKELAAEYFTERREIGIINIGEAGIVSVGEESFHLAHKEAL
                     YIGKGRHDIVFQSDSTQKPAEFYLLSYPAHQAYPTRKVTQAQANRRDLGSDAECNKRT
                     IFQYLRPGVLDTCQIVMGLTELAEGSVWNTMPPHTHERRTEIYLYFDLPKNGLVFHLC
                     GEPESTRHIVMHNKQVVLSPSWSIHAGVGTAHYTFIWGMGGENQDFDDMDDIDPVNLR
                     "
     gene            complement(58894..59544)
                     /gene="eda"
                     /locus_tag="PKX09_01635"
     CDS             complement(58894..59544)
                     /gene="eda"
                     /locus_tag="PKX09_01635"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOU16563.1"
                     /translation="MDRQTITAKLIESGLVAVIRLDSGEKLPGIISALRQGGINALEI
                     TMTTPGALEIIKNFAAQTGKDFLIGAGSVLDAETARLAILNGAQFIVGPIFNPEVIRM
                     CHRYDKPAIPGAFTPTEILTAWEQGADLVKVFPTTALGPSYIKDILGPLPQVKLLPTG
                     GVTLENAAEFIRSGACCLGVGTALLNKKMIAADDWQALAKHAEAFRSAVLTGRSSK"
     gene            complement(59701..60456)
                     /locus_tag="PKX09_01640"
     CDS             complement(59701..60456)
                     /locus_tag="PKX09_01640"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOU16564.1"
                     /translation="MAPKNKSQPIYNLFPRIGNPLSLSQEIVEHIEELIRQKKILPGE
                     KLPSETELCKMFGVSRTSLREALQKLSARGLLRIRKGSGIYVTDYQAENVIKPMSLFL
                     ELNLDKDYLMSVMEIRKILEPQIASLAATRRTARDLTKLRKNCNDLKKCDSTDYLKQG
                     QIDKDFHLNIAIASKNQLVPIIVEPMFRLMPKIRSLVYQQIDSAKSSAITYHDLILEK
                     IQVKDSQGAFEAMTTHLQIAEEHTLQIITDLAK"
     gene            complement(60703..61473)
                     /locus_tag="PKX09_01645"
     CDS             complement(60703..61473)
                     /locus_tag="PKX09_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOU16565.1"
                     /translation="MQEITGYPGAIRFSIEKNNGETPSLAQIFQMRQNNKQLISPNEQ
                     YHQLVLFQHFLRELTAALKYNILKNTSVAFRMRVELANLLKENRALIQPAAIVRLVDC
                     LPTETGLALAGGTYELHGKSLHRFVNKSDPVKPKPIFIFIVTIGSAIDKRIADLNYTE
                     GDAYQAFLLNGLAAALVETATSDLQQYLEKSSSWLSPSQTLRRVSPGFRDWPLTDQKV
                     IFEILKPSENIGVTLSADCLMQPLKSTSGIMGVIENAL"
     gene            complement(61665..62288)
                     /locus_tag="PKX09_01650"
     CDS             complement(61665..62288)
                     /locus_tag="PKX09_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOU16566.1"
                     /translation="MSEHPYFIGIAGNIGVGKTTMTDLIARRFGWRAFYESVIDNPYL
                     EDFYQDMKRWSFNLQIYFLSHRFRSHKQMTESNITTVQDRTIYEDVEIFARNLYEMGN
                     MSQRDWDNYRALFEIMTSYLKKPDLIVYLRASVDTLLTRIKKRSRGFERDISPEYIYT
                     LNLSYERWIKEEQKRSNILIVETDQFDIFRDQEKLEEIMQKIKQFCP"
     gene            complement(62281..63576)
                     /gene="mtaB"
                     /locus_tag="PKX09_01655"
     CDS             complement(62281..63576)
                     /gene="mtaB"
                     /locus_tag="PKX09_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOU16567.1"
                     /translation="MIATVAFRTLGCRLNQAETDAIKAELAHRNLHIVNSNKPADLTI
                     INSCAVTQAAEAKTRGAITAARRISPNGKIAVIGCYSQIAAAELLNIPGVFLVLGNNE
                     KYEIFDFLETMERGEKIVRVAKPEFPKTFRIESHLPNDKRIRANLKVQEGCDYYCAYC
                     IIPHLRGNPRSRDPEDCIATARLLENQGVQEIVLTGINLGRYESPSGDLSFLIRHLLA
                     ATGLPRIRLSSIEPDLITSELIKLIYEEPRLCRHLHIPLQHGSDPILAKMNRRYLSAD
                     YATLINRIKQQIPEICIGADVMVGFPDETDLEFDQMRQFLENLDINYLHVFRYSPRPG
                     TTAGKFTDRIPDSEKKRRSEILRQLSNRKRAAFRQSMLAKTVSVLFEEKIAPGLWQGL
                     TDNYLKVRIHTRENLLNKIEKVKLIHVSEDYIEGELLGE"
     gene            complement(63573..64643)
                     /locus_tag="PKX09_01660"
     CDS             complement(63573..64643)
                     /locus_tag="PKX09_01660"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HOU16568.1"
                     /translation="MKALITAGGHGTRLRPITHTQNKHLIPIANKPILNYALEYVRDA
                     GIQEVGIITNKEGNEVREVYGDGQAFGLKIVYIPQERPTGLADCVRIAEPFIGNEPFV
                     FYLGDNIIIGGIRRFIEEFERNQTNCHLVLAKVPDPERFGVPEINGDRIVSIVEKPQK
                     PLSDFAVTGIYIYDHTIFEAVNNIQPSARGELEISDAHQYLLNKGYKVTFSEITGWWK
                     DTGKPSDLLEANRLVLDNITTSIQGKVDQNSQLSGRVVISKGAEIINSNIRGPVIIGE
                     GAKIVNAYIGPYTAIDKNCEIRNAEIEFSIVSCNCVIRNQKIRIEASLLGKNVQIIES
                     QAKPFTNRFIIGDQSIIEIRQT"
     gene            complement(64720..66024)
                     /gene="rimO"
                     /locus_tag="PKX09_01665"
     CDS             complement(64720..66024)
                     /gene="rimO"
                     /locus_tag="PKX09_01665"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOU16569.1"
                     /translation="MSLIPAVRSFAIISLGCPKNTVDSEILKGGLMQAGMEFKADPAE
                     ADAVIINTCGFIQPAKEESIETILETLRLKKTGVKKVVVMGCLSQRYYAQIKAEIPEI
                     DGLFGVESQSEVIRFLTGSQNIGPDLETIRQLMTPRHFAYLKIAEGCDNSCSFCAIPL
                     IRGRQRSRSIESLLREAEYLNSQGVKELILIAQDTTRYGSDLPGRVSLKTLLEELLAA
                     RLFPWLRLMYVNPDFWRDDLNHILAQHPEFCPYIDIPIQHASPRLLKLMNRGANPKRL
                     KTALRNIRRYRPDVALRTSVMVGFPSETEAEFEELLNFIEEIRFERLGVFTYSAEEST
                     VAEKFNDDIPEPEKERRRDLLMQIQWDIGEDFARSKIGQKITALIEEQDGDEYIARSV
                     WDAPEIDCNVRVKSPRILSIGDFYEVTIIGVENLDLRAETNG"
     gene            complement(66121..67005)
                     /gene="ftsY"
                     /locus_tag="PKX09_01670"
     CDS             complement(66121..67005)
                     /gene="ftsY"
                     /locus_tag="PKX09_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOU16570.1"
                     /translation="MINFGLNKTGRFLASRLPLLGRFFRLDEKTLEEIEETLIQTDLG
                     VELTENLIERLRKVDRQEATRLNEILQNELSALIQPAVTTEKNFAKPTVILIVGVNGT
                     GKTTTIGKLAAKYASEGQRVLICAADTFRAAAYEQLRIWAERSGVEFIGNPQGKDPSA
                     IAYDAARAAQARNADILLIDTAGRLHTKSNLMEELAKVKRVIGKVIPDAPHEVWLVLD
                     ATVGQNGILQAREFQKAIGITGLILTKLDGTAKGGIALAIHRELNIPIRFLGVGEKLT
                     DLVEFDPNQYIRDLLNIE"
     gene            complement(66998..67636)
                     /locus_tag="PKX09_01675"
     CDS             complement(66998..67636)
                     /locus_tag="PKX09_01675"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOU16571.1"
                     /translation="MSKNPIKHTLQVKRNITSKYDAIPARKTVTISNIISLVRAFLAA
                     PIVYSLRNNDSKTALIFILIAILSDMLDGWLARSSNEITVLGKILDPFADSVVIMSVM
                     LFLTVKERIPTFYFTIMLVRYFLISVFGIYMLNNSKVSPQSNKLGKVSVVFTVITVLA
                     FIYPNFFGSSAKPLMIVSIIFMALSLVQYLYEYSRQIIIANREKKTNLSKND"
     gene            complement(67617..68381)
                     /locus_tag="PKX09_01680"
     CDS             complement(67617..68381)
                     /locus_tag="PKX09_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HOU16572.1"
                     /translation="MNYLICQNLEFQYPRNPDFFLSIPELTCPLNYPLGVYGLTGSGK
                     STFGKILSGLLIPTKGFFQIETSEPFSSDKKIKPRILYLPQFPELIFLGMSIGNAWQK
                     ILKNWNDPADLTIKFADNLRKLGLDYSLIRERYGYELSSGELRLATIALGITLAPQLT
                     IFDEPTIALSPSGRMKFENILKEYDPQKEFMIISHDYRLLRKLCRNVLIFNQGSVIFE
                     GEWKTLYQNHSIIHEVGLNLLEKLYQRDIYNEQKSN"
     gene            complement(68400..69047)
                     /locus_tag="PKX09_01685"
     CDS             complement(68400..69047)
                     /locus_tag="PKX09_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU16573.1"
                     /translation="MILRLDGIDFSYHGNQPVFKNLNLTIPLNCWTVLTGDCGTGKTT
                     LAKLIAGLLKPDKGSIRFENDNGLNNSLEVGYIFQNPDDQFVHFNIEREVAFNLENRG
                     ASPAIVRKAVQLALESVNLWPRRENSPHELSGGEKQKLTIADWLVSKPQVLIFDETTA
                     FLDLPTRFELYENIRQLIVDGLSVIWISQEDYEIAFADYIIELLPGYLLRTSSNK"
     gene            complement(69044..>69502)
                     /locus_tag="PKX09_01690"
     CDS             complement(69044..>69502)
                     /locus_tag="PKX09_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16574.1"
                     /translation="HEPNDYALEMLEDEFQTHNIETVRYSQLKGMPANVRVVLIDRIG
                     ILAELYHQANLAFVGGSFRGSIHNVMEPAVAGLPVLFGPSYHNSQEAELLVQSGGGLV
                     CLDEPGIYSTIKHLITKPDDYQKAAQSARQVILKNMGASSRTVQEILESE"
BASE COUNT        20249 a        14569 c        14743 g        19941 t
ORIGIN      
        1 tttcggccgg aaattaatat gcagattgcg gaagggattt ttgattttgg ggcgtggcca
       61 atgccagttt gccgaacccg tccgacgatg ccaaccggag agtaatttgt aacgagctaa
      121 gtaaagataa ccaattacca gtccgccgag atgggttaga tgactgatat tgcctgtccc
      181 cggggacaag gtcgaaaaga aagtaattac gaccataatg atagcaaaat atttggcttt
      241 aatcggaatc aggaagtaaa agtaaatata tcgttccgga aagagcatcg cgaaagccac
      301 gagcacacca taaattgccc cgctggcacc gaccaccgga atgtcagaat gagcgctgaa
      361 tagcatcgtg atcaacccag aaccgactcc cgttattaaa taaaatttgt aaaattcgcg
      421 tttgccccaa taattctcga tttccatgcc gaacatccac aacacaaaca tattccagaa
      481 aatgtggccg aatccaccat gaataaacat gtaagtaaaa aattgccaaa tatagccctt
      541 tttcaggacg gcgtagggta ccaatccaaa attaaaggca aaaaaattct gagcctgaaa
      601 aatcaccata atcaaataaa tcacaacatt agcgataatg atattgccca cggcgcccct
      661 cgaaaaacgc gggcgaatat aaccggattt ataacgcgat tggttgtaat tgtaataatt
      721 cattggctaa tcctttctat cggctgactg gataatttgc gagagaagtt cgaagctttt
      781 caatctgacg ggtaaatcgg tttgcattgc taaataattc accaaagttt tggcaatcgc
      841 cataattggc aaatttaggt tgcagagcgg caaaccggtg cggatatcag ctttttcaat
      901 ttttttcaga atctcaagtt cctccataga aaatggtaag tttgaccagt ccgaatttct
      961 actggaattg agaccgatct cgtatcccat cagcgtcgtc aaattcaata taaaataaac
     1021 aaaagcaagt tggctattat cgttctgcgc ctcgatataa ttcaaagtgt ccaaaaccaa
     1081 tttaaaaatc ggtacatcct catggtactc gcgaatgaac ttttcaatac attccaaaat
     1141 ggcggttgcc ataatggtcg gctcaatttc agtactgttg gacaaaaaac tccggatagt
     1201 ttcggcttgg ctgagcgtct gaatatcgcg agtcggcttg taataaaatt ggacttccag
     1261 taaacttagt ggctccaagt acccgcggaa agggcttttc aggttgcgag cgcccttggc
     1321 aagaaccgca actttaccgt ttgattcggt aaacagacgt gcaatcaagc tagtatcttt
     1381 aaacggaata gttttgatta cgaaggcgcg cgttctaatt attgccatta ttttcctctg
     1441 gattaatcct aaatcaagcc atctcgatca cattttctta ttgaaaataa aagtattcac
     1501 ttttggactt cctccattag tcacaataaa tcataataaa tcacaatgaa tgacaacgaa
     1561 acacgattaa tgacaatttc ctgactcccg aaatactacc aatgacgact gagctgctaa
     1621 ttgattgccg tcgttaaatt ttaataagct cgagcaaaga tgacaatccg gtcagtggtt
     1681 ttgccagtat aaaaacaagt gcctttttct ttttgctcaa atggaatgac ccgaatagtg
     1741 gctttggtct cttcttgaat gcgagcttca tcagcgctat tgcccgccca aaaaacccgg
     1801 atgaatccac ccgcctcgat taactctttg aattggtcat aattctcagc ctgaaaggtg
     1861 ttattttcgc ggaaggcttg ggcgcgtttg aagaggttag tttgaatttc ttccaataag
     1921 tttttaataa ccgtcggtgc attatcaacc ggaataggtt gtttacgtcc gatgggttcc
     1981 atctcggttg aatcacgacg aacaagaacg acctggtttt tagcgacgtc tttgggaccg
     2041 acttccagac gaatcgggac ccctttcatt tcataataat tgaatttcca gccgggagtt
     2101 tcctgttcgg agttatcata acgaaaccga atttgttcgg ctttcaatct ggtggtgaga
     2161 ttagcgacaa attcattgat tgcgcatttt tcttcttcgc ttttccagat tggcacaatt
     2221 acgacttgat acggcgcaac gcgcggcggc aaaattaatc ccttgtcatc gccatgcgtc
     2281 ataatcaatc cgccgatcaa gcgtgtgctg atgccccagc tggtagcata gacatattcg
     2341 agctggttgg atttatttaa aaaggtgacg tcgaaagcct tggcaaaatt ctggccgaga
     2401 ttatgcgaag tcccagcctg cagagctttt ttatcgccca tcattgcttc aatagcgtaa
     2461 gtgtgaactg caccggcaaa cttctgagct tcggttttgc gaccgaccaa aaccggcatg
     2521 gcgagataat cttcggcaat ctggcggtaa atttcgagaa tgcggcgggc ttcttcttcg
     2581 gcttcttccg gcgtggcgtg ggcggtgtga ccttcctgcc ataaaaactc ggtcgttcgt
     2641 aagaaaagcc gagtgcgcat ttcccagcgg acgatattcg cccattgatt gatcaagatt
     2701 ggtaagtcac gataactgcg tatccatttt ttataacttg cccagatgat tgtttcggaa
     2761 gttgggcgca catacagcgg ttcttcaaga ggtttgccgc caccgatcgt tacaatggcg
     2821 cattccggcg caaagccttc gacatgttcg gcttcgcgct tcatataact ttccggaatg
     2881 aacattggaa aataggcgtt gacgtgacca gtttctttaa acatccgatc agcgatgctc
     2941 tgaatatttt cccagatcgc ataaccgtag gggcgaataa ccatcgtgcc tttgaccgga
     3001 ccatagtccg ccatttcggc tttcaaaacg acatcagtat accatttggc gtaatcttcg
     3061 gaacgctttg taacactaat tgacacgtta atttccttga tttatttcgt tgtttttttc
     3121 tttgaaaccg atccgatgga atggattgac aatactggtg caaatattat agagatgcgc
     3181 cccgacacgt ttcaggaaac gaatatatag cgccaaagtc acggcatcgg aaatattaaa
     3241 ttcgcattcg cctttgacca gagtgttgat taaaatggtc gtatcgcgtg agacatcttt
     3301 atactgatga acaacctgac gagcgagttc tacatttgat tctggaaaag ctttgatcgt
     3361 ccagtcaaag aagtccttga cccgtttttc gatactgact acagtctcct caaactcggc
     3421 ggcatggaga gtctttttat gcagaattgc caaatcagcg atgtttttgg tataatcacc
     3481 aatacgctcg atactattga cgacaatggc aagcgttaat ccggaaatga cctcgctttt
     3541 atcggaaatc atcagatggg tcatcaattt tttacgaata tcccgcacca atttattaat
     3601 agttttatca tgttgataaa tgtcgaggcg caattcccca gtgtcacttt cgcgtagaga
     3661 ccacattgat tctacaaaca tctcgcgggt cttagcgagc atatcaaggg cttgatccac
     3721 ggtttccgca gtcaaactct tttttcgcca aatttttaaa aattcatcaa acatatctta
     3781 cctcgttata aatataataa ttaatggtat gataaagaaa agcaagataa cataggcaat
     3841 ggcatataac cggtttttac gggttaaact ggctaataac cgggctagct caatgggaat
     3901 tttttggagg ggtaggaaaa tacatgcgcc gattacattg aataaagtat gagcaaaggc
     3961 aatcgtaatg gcatcaaccg aattagtggc tatagcggca agaaaagccg tgattgtcgt
     4021 gccgatattt gccccaaccg catagggaaa aacctttttt tgggacaata ctccggcggc
     4081 cgccaacggg accgttaaac tggtggtaat ggaactactt tgaactatcg cggtcagcaa
     4141 caagcccagt aaaaagccgc gagtagcatt tttaaaaata tactgatcaa aaaacagctc
     4201 gactttcgcc agaacgagcg atttaatcac tttgactaaa aatgttagag caaagaacag
     4261 aataactaaa gccaaaacta tattgatcca gggataattg ggaaataaat caataataaa
     4321 ttgcgataca ggttccaaaa ttttactaat tggattgata aacttcaaag cgccgatatt
     4381 ttcaaaagca gttgccacag aaccggcggc aattccgaga atgttaaaga aatattgaaa
     4441 tgggaataca attataacgg ttaagacatt gaaaaaatca tgcatcgtag caactgcgaa
     4501 tgcccgctca aactcatctt tttttccaat atgtcccaat gccactaaaa cattggtaat
     4561 ggttgtgccg atattcgcac ccataatgag tgggattgcc agattgattg tcagaacatt
     4621 accggctacc atactcacta ctaaagaagt cgtcaatgag gaactttgca caatagccgt
     4681 gcaaagcatc ccagcaaaaa gacccacgaa cggattagat acgccgttca taattttttg
     4741 ggcaaagccc tgacccatca ttttaaaggt atcaccaatc agggtgacac ttaagagaaa
     4801 aagataaatc agcgttatga ttgccagcgc cttggcaaat ccaacccaat atttcctatc
     4861 cctaaaattc attccagtcc ttttatatga ttaaataatt tcaaaaatcg ggcgaaattt
     4921 agaaaaaaaa cggctaataa ccgcattttt ggtttgtctc ttcttaatta tttttacttg
     4981 tttttaagtt atatgttaaa attttataat gagttgaagt aatactccta atccacccat
     5041 ccgttaatct ttctcaaatg aacttaaaat cacttaatcg cactttaacg tgcattatta
     5101 gtgcgtgttt tgtacaaaaa tctttcctaa ctttggatgt tttttggata tttttgtaaa
     5161 aataaccact ttatttataa agggatcggt atgaaatcgt ccaacatatt tgatgtcata
     5221 ttaatcggtg ctggaccggc aggtttattt gcggctgaaa ccctggctgg caaaagttta
     5281 tcagtgctag ttttagatcg cggtgacgaa ccgctgaagc gtcatgatgt caatttcgga
     5341 attggtggcg ccggggcttt ctcggacggc aaactaaatc tgacttctcg gattggtggt
     5401 gaaccggaaa gtttcggtcg caaggctgat ggagtagagg aattgattca gcaaattgat
     5461 gaaattttta cttcgttagg agttaccggc ggctattcag gagaaaatgg cgaggcttat
     5521 caaactttat tgcgggcggc gcatgcggcc ggggtggaat tcattttcgc caaacagcgt
     5581 catattggca ccgaccgcct gcaggatatt atccagcgct tttatgaacg attaattgag
     5641 aaaggaattc agttttgtcc taatacctct gttgataaaa tttccagaga gggagaattt
     5701 tttaaattga cctgcaaaga ggaagtctat tttgccagag cagtgattgc ggctcccggc
     5761 cgggcgggcg cttactggtt gcgggaacaa gcccataatc tcgggattca cacgatgtat
     5821 ggaccgattg acgttggagt gcgggttgaa tttccagatg atatttatcg cccaattgcc
     5881 gccgttatgt atgacgctaa gtttcgactt tatagtaaat cttatgatga tctggtacgc
     5941 accttctgca cgaatccgag tggctatatc gttacggaaa aatatgacga ttttgtgttg
     6001 gttaacggtc atgccaaatg gaattccaaa accaataaca ccaattttgc attactttcg
     6061 cgagtcgtat tgaccgaccc ggttgaagat acgaccaagt acggacgcga tattgctcgt
     6121 ctggctacca caatcggcgg cggtaagccc attatccagc ggctgaccga ctttgtttcg
     6181 ggcagacgct caaattggga acgcattagc cgctccgcta cggtaccgac tttgaaggat
     6241 gtcacgcccg gtgatattgc gatggctttt ccgcaccgca ttgtgaccaa tttgatcgaa
     6301 gggcttaccg tattaaacaa aattatttcc gggctgatgt ccaataatac tttgctatat
     6361 gccccggaaa taaaatttta cgataccaaa tataaagtaa cgccctattt ggaaactaca
     6421 cttgaaaatt ttttcgtggc tggtgatgcc agcggacata gtcggggcat tgtctattcg
     6481 gcggtgaccg gtatttttgc ggcgcaagga gtcctaaaaa atttgggcaa agattaagtt
     6541 cagcctgtca ttttttaatt cgatctagtg cgagctgtat aaggagagaa aaatcttgaa
     6601 atcgccggag aataaagata agtttatgca agagggactc gaatttatca agcagatagt
     6661 taaccgtcgc ggtagtcgcg acactaaact actggcaatt tgccgctttc tcaatgataa
     6721 atttatcggc tttgattggg ttggatttta cttcagtgat aatctcaaaa aggagctaat
     6781 tttgggaccg tttatcggag atcccaccga acacaggaat atcccatttg gtcgcggagt
     6841 ttgcgggcgt gcggcggttt cgggcacaac ggtcaatgtg gctgatgtta cggctgaatc
     6901 gaattatctg gcttgtagtc ttgaggttcg gtcagaaatc gttatcccta tttttaaatc
     6961 cggtcagttc gtagctgaat tagacattga ttctcatcaa tcggatcggt tcggtaattc
     7021 cgaaagacaa tttctggaac aagtggggca aattatatca gttatttttt agatataggc
     7081 aacaattatg ctaataaaag gttaaattaa ggtttgattt ttaatttgct atttaaatga
     7141 tttataaaag aattaactat gttagattga gacaaattat gcgcaaggca accattccgg
     7201 ttataataat cgtcgttgtg ttaggatttt tatttatttc cccgtttggc aagtcaatca
     7261 ttgcctcggg gagtgactta taccaaaaat taaacatttt tcaggatatc attcgaatca
     7321 taaatgacag ctatgtggaa gaaccggatt ggaataaagt aatggaaggc gcttaccggg
     7381 gaatgctgga agaattagat ccccattcgg tttacatcga gcccaaacaa ttggatgatc
     7441 taaatgagca attttccggt aaatttgaag gaatcggtat tgaattcgat attttggacg
     7501 gttatataac ggtcatatca ccggttgccg gagctccggc tgaaagagcc ggactggaag
     7561 tcggcgataa aatcgtcaaa attgatggtg aatccgccta tcgtattagt cgcgatgatg
     7621 tccagaaaaa actgcgtggt cccaaaggct cggaagtaac cgttacggtt cgtcgtctgg
     7681 ggcaagccga ttttgatgtg aagattatcc gtgatgaaat cccgctttat agcgtttcgg
     7741 cggcttttat gattgatgat agtacgggtt acattttctt aaatcggttc agcggaacga
     7801 ccagtgacga agtcgaaaag gctctaaacc gattatccgc ggaaggcatg caacgtctgg
     7861 tttttgattt gcgcaataat agcggtggtt atctggaaca agcggtggaa atcgctgata
     7921 aatttatcgc cggtcgccaa aaaatcgttt ataccagggg acgaatttcg agtgctaatg
     7981 aagaatttta ttcaagtaaa accgaaccct atgagaagat acccctggta attctgatta
     8041 accggggttc agccagcgcc agtgaaattg ttgccggtgc ggtgcaggat ctggatcgcg
     8101 gactaattgt aggcgaaacc agtttcggca aaggcctggt ccaacgtcaa tatccattga
     8161 gagacggctc ggcaattaga gtaaccgtcg ctcgatatta tactcccagt gggcggctga
     8221 tccagcggcc ttacgatggg gcaattgacg actattatga cacttttagt gcagaaaacc
     8281 gtgattcctt gttagctgtc aaggattcgg tcgagaaacg gccggaattt agtaccattg
     8341 gtggccggac ggtttatggt ggaggcggga ttacacctga ttattcggtg aaatatgatc
     8401 aagcgttgaa taatgagacc tataaattat tgagacatcc ctcgcgagtt atttttgaat
     8461 atgcctccga ttttgttaat aagaatcggg atttagccgc cgatcgtaaa aatttttata
     8521 aaaattttga ggtcagcaat aaagatttcg aggcatttaa gaaaatggcg gtcgaaaaaa
     8581 ttggcgattt gaatattaag caagttgaga aagatgctga atatttgaaa attttaatta
     8641 aagccgaaat cgctcatggt tattggggct acgatgaata ctataagatt gtgcggcttt
     8701 cggataatca agtcacgtct gccgttgatt ttttgaatga agccagaaaa atgcttcaga
     8761 ttcaataatt attacttgag aatatcattg acttttttaa attattgagt taaaattgtc
     8821 agcattaaaa attgaaatta gaattgtaat tatggaggta aataagtaat ggttgaactg
     8881 tttcttagag gcggaatttt catgtggcct attctagcat tagcaatatt tggtatcatg
     8941 gtcgtgattg aaagggcata tacactgtct aaggcttcgc gggtaaccaa acgctttctt
     9001 ccaaaagttc ttaatacttt gaaagaagaa gatggggtca ggaaagcgat tgagttatgt
     9061 gaaaatactc ctggtccgat agctgagatc ttccatgctg gtttatcaaa agttggatat
     9121 ggcatggaag ccgttgaaaa ggcgatccag agtgctggca ccgtcgaaat ggcatttctt
     9181 gataaaggta tgatttggct ggcgaccgtc attaccctgg ctccgttact gggctttatg
     9241 ggtacggttt ctggtatgat caatgctttc gacgccatcg caaaagtcaa tgatatgtcc
     9301 cctgctattg tagctggtgg tatttctgaa gctttattaa caaccttatt tggtttaatt
     9361 gtagcagctc cgctacagtt atgttataat ctattcgtag cacgcgtgga cagtattgta
     9421 gtcgagatgg aagaaagtac tatgaattta ttgcaaactc ttgatgttat taataaggaa
     9481 aattgcaagt aaatatggct ctgctgaaaa aaaagaaaac tcgcaaagtt gatattccat
     9541 ctgcctcgca ggcagacata gcctttcttt tacttctttt ctttatttgc acgaccacgg
     9601 ttgatgtaga taaaggcatt ggactgatac tcccacccga aggtgacagc ccgatagaga
     9661 tttctaaaaa gaatatcact acaatcgtaa ttgatccgca aggcaacgtg ttaattgata
     9721 ataaagaagt tgaaattaat cagattcggg atattgccga aaggttacag atagaaaatc
     9781 ccaaaatgat cttttccgtt aaagctcatc cgcggtgccg atacgataaa tacgtggctg
     9841 ttttggatca attaaaaatg gctaaagcta ccagaatttc tattgctgaa taaggagtaa
     9901 tgaaatggct tttcaacata aaaaaaatat cagtacggat attccaacag cctcaatgcc
     9961 agatattatt ttcacgttaa ccttattctt catggctacc acagtgatgc gtcaatattc
    10021 tggtttaccg gtagttttac ccaaagccaa acaaattgag aaacttcagt ctaagatcca
    10081 tgtaaccgag ttgtgggttt ctaaagagga attgatttca atagatggta agctggtaaa
    10141 tctccaggac attagaaata ttatgtataa caaacgtgtt agagatccgc agttaattgt
    10201 ttcgattcgg gctgatgaaa gggttacgat ggacttaatg agcaagattc ataaagaact
    10261 gcggttagcg gatgcgctta aagtcaatta ttccactaaa acagctagtt aagggttaga
    10321 ctatgatttt aagaaaaaaa cccgaagcaa gtttgaaatt acgccaaccg ctgatgtatc
    10381 gagttggact ggttgtcgct ctggtattaa tcacagggat tatgattgct ttccccaaat
    10441 ttgaagaacg cgaagtagtc cagagaaaag ttgatattat tttggaagta actgatattc
    10501 cgcaaattga acaaaaaatc gaagccgcca aaccaccgtc tcgcccatcc atcccgatcg
    10561 agtcggaaaa cgaagacctt tctgaagaca tcacaattga tcagacgacc gattttgatt
    10621 cttatacagc ctgggaggca ccaccaccgc ctccggaaca aaataacgac gagggtgaag
    10681 gaccgcgcgt ccgatttatt ccctatgatg aacctcccga gccgattggc ggatttgccg
    10741 agatacagaa aaacatagtt tatccagaaa ttgcgcagga agccggtatc gaagggacgg
    10801 ttgtagtcca ggcttatgtg aatgaatacg gagtcgttac cgaatgcacc attctaaaag
    10861 gaatgcccaa cacgggactc gaagaagccg ctatcgccgc gataaaaaaa acccgcttca
    10921 aaccggctaa acaaagagac cgaaacgttg gtgtctatat ttcgatcccg gttattttca
    10981 aactgaaaag ctaatcaatt agtcctaaat ttttacctga ccggcataat ctcaattatg
    11041 ctggttttgg tttttaatta ttagtcgaaa ttctgaccag ttaatttgct tcattggtga
    11101 agacaatctc cttggttttt atctatttat taacgatacc aagagtctat tgaatgtttt
    11161 tcttcattac tcgttttttt gtttaaatca tttgccaaat ttggactttc tgtgtaaaat
    11221 gaattgaaga tttagatcaa agtcaacggg actaagtgaa tttaagtaaa cctacagaga
    11281 accgtcctat ttatgccaag acttggcaga atgtagccgc tgttgaacgc tatgctctta
    11341 gccgctatcg tcattatgat cagcgcttaa tttcctggcg ggaaaaacgg atcgttaatc
    11401 atcttctaaa acaaataaaa aatggaaatc acatattgtt agatattcct tcgggttacg
    11461 gacgctttac ccctctcttt cgccaacacg gtttcaaggt tatcaatggc gaccttaatt
    11521 tgtatgctct actttatcaa cggcaacgtt ttcccgatag taacctaatg ttcgtaactg
    11581 atggcaattg tttaccgttg cctgataact cagccggtgt cgtttttaat ttcagacttc
    11641 ttcaacattt ccagaccagt gaccagagaa ttgcactctt acgagagctt gcgcgggtca
    11701 caaattgtta cctgatcgta tcggtttatc ttgaatcgaa ctttcatcgt ctagctcaat
    11761 tagtttcacg gcgtaaacgt cggatgacta tgatttctcg aactcagtgg gataccgaat
    11821 tgcgttcatg cggattgaaa gtactccgga ttagtcgtcc gcttcgtttt ctccacgctc
    11881 aaaacatttt cctaatagaa aaatcgcaat cttgttcggc accaattgat aaataattaa
    11941 ctatttataa attgaggtga aggatgcaaa agattatatc gtttatactc acctgtacag
    12001 tgctagcaat tgccgcagaa ccgctggatt tattaacggt atttaccgaa ccctatcttg
    12061 ccggctcggt agcggctaac gtaacgtggt cgccgaatga ttccctgatt gcttttctgt
    12121 ggaatgatca aggcggagaa tttcgcaatc tctggatagc taatccgcaa acaggtgcga
    12181 aagaacaact gaccgatttt aatccaacta cagacgtaaa tatgccgtct ataggagcgc
    12241 tcggttggct gaataatcgt tgtcttttct tcgagtatca aggtgacatt tgggaggcaa
    12301 gtctgttgcc aggttaccca ttaaaacgga tcgaaggctt aacgaattgc aaaccaccaa
    12361 ttgctcttgc gccgaataga agatatttaa cctacaacag aggtggctat cttagattgt
    12421 ttgattttat aactaaagaa gaacagacga ttattccgga taaaacttat cagcgtccac
    12481 ctgccagaca ggtgtgtgct taccagacta cttattgctg gtcgaatgat agtaaaaaga
    12541 tagtggtcta cttagaatcg gaagccgatg aaaatactgt tcattttaaa gttttcaccg
    12601 tcccgaaaag ctcgaccgat gatgttgtga ttaaggagca tccgagtgac aattccattg
    12661 tgcgcaactt tatctggtcg ccagataatc agtcaatagt tattgaatcg gttacttcaa
    12721 atttacgtga aagatatatc cttttagttg atctgacgaa agaccgattg gatactttat
    12781 atcgtgaaat tagcaataat tggattgcgg atttcggata ccaattatat tggatagagt
    12841 cggaagcgaa gattttgttt gggatggaat ataactctta taatcattta tttctgcttg
    12901 atccgactaa tgggcactat tccgccttaa ctcgtggcaa atgggacgtt aaggattatg
    12961 ctgtagatgc tacagggcag ttggtatatt ttacagcgac aaaagacaat cctgaacaat
    13021 tgcaattata ttctgcccaa atcaaaacgg gtgagattac agatatttct tatcgaggtg
    13081 gtaattactc attccaatta tccaataata gtcgttggat agcggaggtt ttttccaatt
    13141 actctacccc gcccgattta tattggatcg aagcgtctcc ccaaagtaaa atgaaccaaa
    13201 taactgatcg tccagattca ccactcgaaa aaccgctgag gaaaattccg aagagtggaa
    13261 caattcagaa tgcaataacc ggtaaaccag ttaattaccg cgtatggtta cccgacgatt
    13321 ttagagtaaa tcataagtac ccgattatcg ttgtattaaa tggtgctgat tgcgtagcgc
    13381 cgctgaccga agcctggcga ccggaatgtt attttaagca atggctgagt gataaaggtt
    13441 atattgttgt tgctgtgcaa tatacggatt taaggacagc tattgaacca gcggactcgg
    13501 aatcgttcag cgatcctttg aaaattcaat tagcagatat tcaggctatt gtcaaagaaa
    13561 tcggtaaact ccaatatgtt gatttgtcac gaagcgggat ttgcggttgg ggctatggtg
    13621 gctacttagc cacgatggct atgttcgaaa ctgataatat ctttagagcc ggggttgcga
    13681 ttttaaatga cttctcctgg tcacaatcat cgagtatcta tttgaattat atggctaatc
    13741 tactagctaa tgaccaggtt tttagaaaaa ttgatccaca taatttttgc tataatctaa
    13801 ggggacgttt gcttgttatt cagagcgcat ccagcacttt acctcaatta gtagatgccc
    13861 agcggttaat tcctgaattg ttatctatcc gtagaccggt agatttttat ctttatcctt
    13921 gggaaaataa tccgattatg aggagcgcca atcaatatga tatgtttatt aaaacggcgc
    13981 gctttttcca gttgaattta tgactctggc tcagaaatga ggttgttcaa ttaggcaatt
    14041 tgctttcttc cgaattggga aatccaaaag gttcaaaaga ttctttgtcc gggagtttga
    14101 tctcaccaaa tttggcttcg aacttggcga ggttatcctg caaagtgaat agtaacgtct
    14161 tggcgtgaat tggagtcagg attattcggc tttgcacaac ggctttgggt gagccgggca
    14221 tgatgcgtgc gaaatcaagc acaaattcgg cgttcgagtg cgaaatgagt ataaaattac
    14281 tgtagttacc ttctgccttg gattctggca attcaatttt aatttgcgcg acaggtttag
    14341 ttgagttcat taatctttcc ttaaaatgat acgagggagt taatattcat cttcctcgcg
    14401 atcttcattg tcttcgtccc attcgtcttc gaagggaatg tcagtatctg agagatccca
    14461 ggcgtcatca ctgctttcaa tttcttcagt gagattttca tttaactcaa ggtcgtcctc
    14521 gaattcatcc cagacttctt tctttttctc aaatttggat ggatcaattt cttcttcgtc
    14581 gaaatcctcg cgttcgtgtt cgagcatttc tttgccatac aggctcatat cgaagaaatc
    14641 aatatcatca ataatgtcat aatagaccaa cagttccaga aaatccagat atttggaatc
    14701 ccgcaggtta gtcttgcaat tcgggcaaac cagagattgt tttaaatctt cctctttgag
    14761 aggcgcatta cattcggggc aaactaaatc ttccatataa tttcacctta tattacggcg
    14821 ctaaatatac acgccgaagg tatatgcgtc aagttaaatt tttatttact ttttggattt
    14881 gcgggtgtaa taaatcccaa ataagattgt aggatttttc aactaatcta tccgttttat
    14941 aattaataat taggttgaat aatttttccg ctagttcgct ttgaaaaccg accttgatcg
    15001 gtgcgcggca gcgggaaacg ataaaagacg tagtaaccgt cggaaccaca tttaaatcaa
    15061 ttgcaaactc gaggttgatt cgagctaatg ccagtacttc tggatcggtg ggaaaaccct
    15121 ctttgttgat ccgactggaa atcagaggta tcaataaatc gggattaatt tcctgaatta
    15181 aggtaaaaaa tgcttgcggt acgagcaatc ttaccaactc cggtttttgt cgtatgaaag
    15241 gtaaaaaaac tttcagcgcg ctatagaaat gagcgtgctc aaagggtaag caaatcagaa
    15301 ctcgagcgaa tttagttgat tcggactctt ctaaagatgt gtccaaaggc cagctaaaaa
    15361 tcttatgaat gactttatga ttcaagacct ttgattgttg ataattagta tattttatca
    15421 aattcaacct taaaaactga gccgaaaata acctttaaaa tgaaattttc caacgctaaa
    15481 aatttgccaa aaggtcattc aatatctaaa tggattttag ttacaaggaa aagaatagtt
    15541 taaaacataa aagttctcaa tgttagattc ctgacagctt ccaggattgg ttggtagttt
    15601 actgctttag tgaaaataat tttataattc tggtaatcag aaactattag taatcaaact
    15661 aaattattgt aatagccact aactggaaat tgtatgactt ttagtgatta ttctttccct
    15721 tttaagcgca caatcgtcgc accccagccg ccaccggatt catcggctaa ctgaaatttt
    15781 tcaacgtaag gcaatttgcg tagaatagcc tgaacggtct cccgcaatac gccaatgcct
    15841 ttgccgtgaa tgatacgaac gtcgagcaag tctttagcac ggcatgcctc aagatattcg
    15901 ggaaccaagt ccttgatttc tttaggtgag aaggtatgta gatcgagaac gccgttgatc
    15961 ggaataggat atgttttatt ctctgtaatt tgagcaaacg cctcgcgcgc tcttgagata
    16021 aaatgccgca cgagtttagc gtccttccat ccagcgggat tctccacccc agtatgagta
    16081 tccactccgg ccggttttac ggttaaaatt gcctcacgaa tatttatggg atttaagccc
    16141 ccagctaaaa taaccggttt aggtgaatat gcgacgattt tccggctgat ttcccaatca
    16201 tgggtctggc cggtggcgcc ggtcgcgccc gtcttaggat cgtatgtatc tgtgataaaa
    16261 gcttcaacca gactggcgaa ttgatcaata gtatagcgca attcatccat atttccagaa
    16321 cgcacgatta aacttttgat gattctcaga tcgggattta tgcgacgtaa cttttgaact
    16381 tcgttgggta agatgaaacc gtgcagttgc aaatatttta ctttgagcaa acgactcagg
    16441 ctcgcgattt cttgcgcatt atcgagataa gtgattaaga cacttctatc gccgcaggcg
    16501 agtttggcga tgatttcggc ggcttcgact tcggttagat ccggttggtg ataagccagc
    16561 cgcaatggga aaccaaggca atccgcaccg ctctcgagta gcattcgagc ttcatctata
    16621 cttcgaatac cggcgatctg gatgatgttt tccataattg aaaattatcg ttccatacta
    16681 actaaaacaa gttgcaaaaa ccaatctgcc gattaggacg gcacgctaaa ttatatttta
    16741 ttgttttgtg gtaaaagtca accgctcgcc gctgaaatcg gcgatctggg cgtatagcgc
    16801 accggtaaaa accaatatat tacccaagta actcgcaaaa atcattttat ttaacaaaat
    16861 ccgtatgcct ttcattatct cttcttttct ttaatacaaa tgtaaaggta attttactta
    16921 gagtcaagtt taagtcagaa atatttactg aaattcttga attgattgat taaattacgc
    16981 cccgattgaa gatctatgaa attaactgaa taaaattctt ggaaagtcaa gtaaatcaat
    17041 gaataaacgc acggcgcttt taaccctcga ggacggtcat gttttcgagg gtttttcttt
    17101 tggctgggaa ggtgcgaccg ccggtgaact agtctttaat accggtatgg tcgggtaccc
    17161 agagagtctg actgatcctt cctattacgg acagatttta gtgatgactt acccattaat
    17221 cggtaattac ggtgtgccag agttcaagcc cgatagcctg tattttgaat ccgatcggat
    17281 tcaggttcgt gggctgatag tagctgatta ttcagcggat tattgtcatt ggaatgccca
    17341 ccagtcgctg ggcgattggc taaaatcaca aaaaattcca gccataaccg gcattgatac
    17401 ccgcacttta actcagattt tacgggaaga aggcacaatg ctcgccaaaa ttacttttga
    17461 caacgacatt gattactatg atcctaaccg ttacaacgtg gtagcggaag taacctgcac
    17521 tgaaccgcgg acgtatggcc agggcaagaa acgaatcgca cttttggatt gtggatgtaa
    17581 acggagtata atctcacaat tgtctcagca cagagttgaa attatgcgtt tgccttggga
    17641 cagcgatttg accaagtatg attttaacgg gttgtgtatt tctaatggcc caggtgatcc
    17701 tcgaaattgt gaagcttctg ttgcctcagt tcgactggca ttagagaata agataccgac
    17761 tctgggcatt tgcctcggta atcaaattct agctttggcg ataggggcaa agacttataa
    17821 acttaaatat ggccatcggg gacaaaatca accggtaatg gatcaaacca gcggatgctg
    17881 ttacatcacc tcgcagaatc atggctttgc ggttgatgat aaatccttac ctgaggattg
    17941 gcgaatctgg tttagcaatt tgaatgatcg aactaatgaa ggcttaatcc accgctccgg
    18001 gcggtatatg agcgttcagt ttcatcctga agccgcgact ggcccgcttg atacgatgtt
    18061 tgtattcgac cgattcctgg atattttata agaggagaga acgcgaaaaa caattcagtg
    18121 ttaattaatg gatattataa tgccaattat aaaaattctc aaaacaaata accgggaaaa
    18181 aagtaggatt gaattatcag tcttggcgat ctcagcgccc tcagcattga aaggaaaaaa
    18241 tggttaaaaa agttctcatc ctcggcagtg ccgcccttaa aatcggagaa gccggggaat
    18301 ttgattattc tggcagtcaa gccatcaaag ccttaaaaga agaaggcatt cataccattc
    18361 tggtaaatcc caatattgct acgattcaga cctcggaaaa tttagccgat gaaatctact
    18421 tcctgccggt taatgccgaa ttcgtcgaaa aagtaattaa aaaaaccaga cctgatggta
    18481 ttttacttag ctttggcgga cagaccgccc ttaatactgg tctggaattg caccgccgcg
    18541 gcatcctcca aaaatatgat gttgcaattc tggggactcc tattcaagcc attaaagata
    18601 ctgaagaccg ccaacttttt atccaacgtc tgcaggaaat tgaggtcaaa actgcacgta
    18661 gtcagatagc cactactctg gatgaagctt tacaagccgc tgaagctcta gctttcccgg
    18721 ttattattcg gatagcgtat gctctcggcg gactgggttc aggcgtctgc cgtaatattg
    18781 aggagttccg tgataaagcc gaaaaagcac tgacttatgc cccccagatt ttgatcgagg
    18841 aatatctgga aggctggaaa gagatcgagt acgaagtaat ttgcgatagt tttaacaact
    18901 gcctgacgat ctgcaatatg gaaaatgtgg atccgatggg cattcatacc ggcgaaagca
    18961 tcgtggtagc gccctgccaa acactttcca atagcgaaag ccataagttg cgttctatag
    19021 cgataaaagt cgttcggcac ctcgggatag tcggtgaatg taacatccag tttgcactcg
    19081 atccgcggtc agaggattat cgcgtcatcg aggtgaatgc ccgattgtcg cgcagttcgg
    19141 cgctggcttc taaagcaacc ggctatccga ttgccttcat tgccgcaaaa ctagcactcg
    19201 gctacggact gcatcaattg cctaataaag taacacgtac aacgactgcc ttcttcgaac
    19261 cggctctgga ttatatcgtg gttaaaattc cccgctggga tttgaaaaaa ttccgcaacg
    19321 tcagtcatcg catcggttca gggatgaaat ctgtcggcga agtaatggcg attggacgaa
    19381 ctttcgagga agctctccaa aaaggcttac gtatgctcga aaccggtgcc aatggtattg
    19441 tcggcaataa cggtgtaata tttgaaaatc tggaacgaga actgagcgaa ccgaccgatg
    19501 aacgtatctt tgcagtcgcg gaagctctcc agcaaggcta tgacgtcgct aaaatcgcca
    19561 agttaaccaa cataaatccc tggtttattt acaaaatccg taatatcgtc gcaatgaata
    19621 cacacttgag ccagtatcga aatcaagaat taccgaccga taccttacta gccgccaaaa
    19681 gacttggctt ctccgacaag caaatcgcgc tcgctatcgg gaaatcagat gccgaagtgc
    19741 accaattgcg tctgaaagcc aatatccgac cttttattcg acagattgac acgctagcgg
    19801 ctgaattccc ggcttatacc aattaccttt acctaaccta taatgccatt cgcgatgata
    19861 ttagttttac cgattccaat gtggtcatga ttcttggttc aggggcttac cgtatcggta
    19921 gttcggtcga atttgattgg agttgcgtga attgtggtca aactctgcgg agactcggat
    19981 ataataccat tatgctgaat tacaatccag aaacggtcag caccgattat gacgaatccg
    20041 atttcctgat ttttgatgaa attagttttg aaaatgtggt ggaaatttat gccaaattca
    20101 gtgccctcgg agtaatcgtt tcgatgggtg gtcagattcc caataacttg gctttaaaac
    20161 tgcaccacgc caatgtccga attttgggca catcgccgga aagcattgat cgggctgaaa
    20221 atcggcgcaa attctcggat ttgctcgata aactcaacat tgatcaacca atttggaagg
    20281 aaattacttc tgtggaattg gcgaaagaat ttgcccacga atatggttat ccggttttaa
    20341 ttcggccttc gtatgtcttg agtggcgccg ctatggcggt ggcttctaat gacgaagaac
    20401 ttgtccaata tttacaacgc gccgtaaaaa tttcgccgga atatccgacc gtcattagca
    20461 aatttctgga aaatgctaag gaaattgaat gtgacgccgt cgcccgtaat ggcgaaatta
    20521 tcacctacgc tatttcggaa catgtcgaga acgccggcgt tcattccggc gatgcgactt
    20581 tggtcctgcc gccgcaacgc acttatctgg aaactatccg acgcattcga attattacca
    20641 gcaagatcgc ccagaattta aaaattaatg gtccgttcaa cattcagttc attgccaaac
    20701 agaacgaggt aaaagtaatc gaatgtaatt tacgggcatc gcgcagtttt ccgtttgttt
    20761 caaaagtctt gaagcgcaat tttattgaca tcgctaccca ggttattatg cacgtgccac
    20821 tcgaaccaat accagtatca ctgttcgaac tcaatcatgt cggcgtaaag gcgccccagt
    20881 tttcctttac acgtttgcaa ggtgctgatc cgactctcgg cgtagaaatg gcttctaccg
    20941 gcgaagtcgc ttgcctcggt gacgatttcg atgaagcttt tctgaaagcg ctgatttcgg
    21001 tcggctacca ttttccgccg cgcgcggctt taatttcatc cggcccaatc gaatctaaag
    21061 ccgaactact ggaatcaatc cgaaccctcc aaaatatgaa cgtgaagctg tacgccaccg
    21121 gtggaacggc aaaattcctc gaagcaaata aggtttcggt tgaaaccttg ttctggccgc
    21181 tcgagaaaaa agaaccgaac gtcattacct atctcaaaaa tggcaaaatt gatttggtga
    21241 tcaacattcc gaaaagctac caggaacgcg aattgactaa tggctattta attcggcgaa
    21301 cagcggctga ttttaatgta atgttaatta ccaatcgcca gatcgcgatg cgcttcatcg
    21361 aggctatctc tcgcttgaaa atggatgatt tgaaaatcag aagttggggc gaatattgag
    21421 gaaaccgctc cgtgcccgaa ttaccggaag tccagactat catcaatgat cttatagccg
    21481 caaatttagt cggtcagaaa gttcttgagg ttgagattca ctggcaaaaa atcatcgccg
    21541 aacctagcgc cgaaaagttc acaaccgaga ttcaaaaccg agaaataact aaaattaccc
    21601 gacgcggaaa atttattgta attgaactaa agccggtcaa atttctcctc gttcatctgc
    21661 gtatgtcagg gaaattctcc ctgacagcgc ccgattatcc aattagcaaa catgaacatt
    21721 taattttcac gctaaatgat ggtcgccaac tacgttatca tgacactcgc aagttcgggc
    21781 gtttctggtt agttaatcag cccgaaaaag taattggtaa actgggcgtt gagccgctaa
    21841 gtgccgattt cacggttgaa attctaactg aaaatttgcg taaacatcac cgccagatta
    21901 aaccgttttt attggatcag acaattatag ctggtttggg caatatttac gttgacgaag
    21961 ctctctggga agcctgtctt catcctttaa gaatggctga ttctctcaat gaccaggaaa
    22021 ttgcatcttt gcataaagct attccaaaag ttctacgaca gggtttagcc aatcaaggaa
    22081 caagcctcgg caacggcaaa ggcaattttc aatcggttgg caagcgcgct cgtaatcaag
    22141 aagcgctgaa aatctttcgc cgtaacggtc aggcttgccc gcgttgcggc gcgattattg
    22201 aacgtctcaa agttggccag agaagtacac acatctgtcc gcattgccag ctaatctgac
    22261 ctatctcaat tattttatct gaatgtccgt ctttttgatt ccctgaaaat ttcctttaat
    22321 tttaatacgt gaataaagat agctttaaac gatttcccaa tttggcagat tttatcatac
    22381 aataattgtc ttgtataaag tttacctaag aatatttcag gtttccacag cgttgctggt
    22441 cataactttg gctgtaagta cttttctgtc tcccgaagcc ggatggggcg ttcctctgac
    22501 tcaaccaata tcgcaaattg tgcgagcgct tagccttgac cactttccaa attcctggtt
    22561 aaatatcggt ttgtggctgg tgttgataac cggaatgtta tcagccgtat ttctaaaagg
    22621 tatgagggat ttcgctccca agtttctgca ttgcttgctg gcggctgttt ttctcctaat
    22681 cttttatgat aaagctttca atcaacgttt tgttatctcg attcaggagg ggcaggaagt
    22741 caattttgcc caatttttga aaaaatcgaa accggaatac aatgttccat tgcgacttct
    22801 cgatttcgat ataaaaatcc atcctgatgg aaaaacagcg gcgtcttacg ccagccgact
    22861 ggtggttaat caaaccgata cagccattct ggctgttaac catccgctca gtatcggtaa
    22921 atatcggtta tatcaaagtg cttttagaaa ggattattta tttgaagtaa tttgcgacgg
    22981 agataccata atttcgcctt ttaatggggc ttgcaacttt aatcagcaag aaattttcct
    23041 gagaggtgtt gatactttag cgcaggcgtt gactgtcgaa attgatcgaa acgaatataa
    23101 aattccaccc ggtgaggtat gcaatgtcgc cggtcgccaa attaaaatta atccgctcag
    23161 tccaaagtac acctcaatca ttgaagtcgc cgaagtaaca ggcttaaagt tcctcgcatt
    23221 ttttagtcta atgtatttag gtgtgctgac ctatatcatt ttatggaaga aaattcgtta
    23281 aaaaggggga atcgcagtgg aacaaccgct tttaatcggc gccttgattt tatacacaat
    23341 tgcttttgtc ggtctgatta caaaagtcaa agaccaaatt tgttccgttg tgatctggct
    23401 agccatttta actcatttag ccgcgctgat ttggcgatcg ctggcggctg ggcatccgcc
    23461 attcacttcg ctctatgaaa ctattcttct attatctttt ttacttgctt tgaggttagt
    23521 aatctggcgg cggcagattg agtctcggta tcgctggata gtgttattga taattatggg
    23581 aatgattata ggcgctttaa taatcccgct ttcgtataaa acgataaaac cgctatcgcc
    23641 tgccttgaat agttgctgga tgtatctgca tgttccggct tacttttttg gctatatggc
    23701 gcttgttctg gcggttattt atgctctgat ctttctggtg caaaataaaa atgaaagttc
    23761 aagcgatgga atgacgttac tgagaaaaat ggataatgaa gtgcaaattg cttttctctt
    23821 tctgaatatc ggattaataa ctggagcatt ctgggggtat cagagctggg gcaattattg
    23881 gtcgtgggat gtcaaagaag tctgggcatt gcttaatatt ctagttttag cattttattt
    23941 tcacttgtta aagccgagag gagtcaagaa agcggtaatc gtaataattg cttttctgac
    24001 agtgattttt acttatttcg gagtgagttt tttattaaaa ggactgcata gttatctttg
    24061 ataaatacta tttttctatt caagacgata tagtaatgtg gtttgaaatc cgggagcggc
    24121 agaaatcttg cgttgtagaa taaattaggc taaattgcat atagtttatt gaattaataa
    24181 atcgcggggg aaacgatgga gcgcagtcaa tttgaaaata tactggattt tgcaattgag
    24241 cgcgaatggg aatctgttcg tttctatcac cgtttgttgc aggaagcgcc atcactggct
    24301 atgatggaag ctattcgagg aattgagctg atggagcgtc agcatatcaa tacattagaa
    24361 caatttcgta aagccggatt caaatcgcag gcaattccat cggttgataa tattcaactt
    24421 ggtgaatatt atgtacctgc agtcgaaaat caaaattatc gcaacatgct tatgaccgct
    24481 attcagcgcg aagaactttc tcataaatta tataaagctt tagccgaaac cagtcgtgat
    24541 cccgaggtag cggatttgtt cgaaaaatta gcttcggaag agacacagca taaaagttat
    24601 tttgagagcc tatacgattc cgaagtgctc aaggaaaatt aaaccgggtg aattggagag
    24661 aatcaagatt tgtcttgatt ttaacttacg gaaattttca gtgaaaattc attaaacatt
    24721 gcgatcggat tttaatttat tcgaaccggc attagagata gaggaaattg ataggcgaaa
    24781 gggcgattta taaatatgaa ggatagtaat atgggaacaa tgattgaatc ggtcaccaat
    24841 gacacaacgc aaagaacagc aaagtcgcca gaagaaatta tcaatatatt taatgcgaaa
    24901 aaagtagacg atgattggtc atttgcggga tacaaaccat cagataccgg caaatggact
    24961 catgcttacc atagataccc tgccaagttt attcctcagt tagttgagaa attaattgcc
    25021 gaatatatat ctcataaaga agcccatata aacgacccat ttatcggttg tggaacgact
    25081 attgttaccg caatttccaa agggtttaaa gcaagtggaa cagatattaa taaaattgcc
    25141 tatctcatca caaaggtaaa gtctacaccg atagaaccag tataccttaa taagaaaatc
    25201 aaccaaattt tgttcaaatt gaaatcttta aatggaacac gaagaacact atttaatgat
    25261 aaggtcgaac ctttaattcc tcaaaaacat attgatagaa taaattactg gtttatcgaa
    25321 gagaacaaaa atgagcttgg gaaaatatta agagtgattt ataatgaaga agatgaaact
    25381 attagaaatt tctttcttgt tgccttcagt catattttaa aaaactgttc tatatggctt
    25441 caaggaagca ccaaaccaac cagagattta aagaaaaatc cagttaaacc ttatgatgca
    25501 ttccgaaggc atttaatgaa aatgcaaagt ggaaataatg ctttttataa aatcgttcca
    25561 gctatagtaa gagagaattt aagtgcatac ttaaacattg aaaacggaga tgctagaaag
    25621 caacccacat ccgatgaaac tgtggattta gttgtaactt cgagtcccta tgtgaccagt
    25681 tacgaatatg cagacttaca tcaattatca actatatggt ttgatttggc aaatgattta
    25741 acggaataca aaaaggaatt tataggcaca tcctacaaaa aatacgagaa taaaagatta
    25801 agaagttcaa ttgctatgga tatagttgat aaaatgctcc caaaatacaa gaaaatcgca
    25861 aaggaaatcg aggcgttttt cattgatatg gaagaagtct tcgacgagag ttttagaatt
    25921 ttaaaacatg gcggtagatg ttgctatgtt ataggagata caaaattaaa aggtgttgat
    25981 atactcaatg ccgaagtttt tgctgagagt atacaatact ctggttttaa actcgacaga
    26041 attataaaga gagagattcc atctaaaata cttccacaaa agagggatga aaaaacgggc
    26101 agatttgcca ataactatga agcaaactct gaagcttatc ccgtagaata tattgttatt
    26161 ggcttaaagg agtgagaaat atgaaattcg aggatctgaa aaagttttat ctggaaaaga
    26221 aaggacagtt tggagcagaa acgtacaaac acatctcaga actgttgaaa gaagcaaaag
    26281 agattcataa acgagattgg ttgaaacacc ctacacccaa tggagaccat gaacagagtt
    26341 ggagagcgtt caaaggaaaa aatctggaaa agttagtcca atacattatc acagatgaag
    26401 tcgaagaaat tggattgaaa gttgttaatg gcaaccaact ggaaagaagt acgaatctat
    26461 ccaaagagtt gagccaggta aaacggaatt tggctattga ctatggagaa tttggattac
    26521 atttaccaga tgttgatatc attatttaca atcctcagaa tcataaggtt atagccgtaa
    26581 tttcaagcaa agtgacttta agagaaagga ttgcacaaac aggttactgg aagctgaaac
    26641 tcttccaaga cgatacaaca aaacacatca aggtttattt cataactccc gatgaagatg
    26701 ggacattgac atctaagaag cctgccaaaa agggcagggc aattgtagaa gtagatctgg
    26761 atggtagcta tgttttgacc gaagaagaaa tagaagagag taacaaagtc aagcttttcg
    26821 agcattttat agatgatttg aagaggttaa taaataatga aaaataatca accaaaattt
    26881 gagacaacaa ctttatggga ttttccgacc cagaattatg gcgatactcc acacggtgat
    26941 aacaaatacc acggggttac gcctgcattt gtaatatgga atcttgttca aagatataca
    27001 acggaaggcg acctcataat tgatcctatg tgtggaagtg gaacaactat agatgtctgt
    27061 aaagaagaaa aacggaaagt tataggtttt gatatagtcc cttatagaaa cgacataaaa
    27121 caggctgatg caagaaattt acctcttgac gacgaaatag cagatttcat ctttatagac
    27181 tcaccatatt cagataacat tgattataat aaacatccag ataatattgg aaacatatct
    27241 tgtgaaaagg aagaattttt catagagata gaaaaagtag caagagaagc tcatcgtatt
    27301 cttaagccga acaagtacat ggcgtggctt atcggcgatc attggaggaa gaaaagcggt
    27361 tttatacccg tcggtttcaa aatttatacc atattagaaa aatatttcaa acctattgat
    27421 atgattgcag tttcaaggca taatcaaaca tcaaacaccc ccttatggca cgagagagca
    27481 aaacaacata atttttacct tcgggggttc aaatatttaa taataatgca gaggatgtcc
    27541 aaatgaaatc gcctcatcgt ctaacaccgc ataaaaggct cgtgccttac ggcactcacc
    27601 caaacctagc tttttttgtg ggagaatttg ggtgcattat ataatggact ttataaatag
    27661 aaaagggctg ggcttctttt atgctggaaa ttgacagagg tacggactaa ttatctatca
    27721 ttgatttgaa attacctaca atttataagg agtaactatg gcagtcaata ttgttgaagt
    27781 ggcaactccc cgacaattaa aacagttcat aaagtttcca ctgaagcttt atcgtaataa
    27841 tccatattac ataccaccat tgaattttga tgaaaggaat acgctggatc cacgccgcaa
    27901 tcccgcctgt gaattctgcg acacgcgtta ctggctggca tataagaatg gcaaattggc
    27961 gggccgcatt gccggtattt acaataaacg ctttgttgaa atttgcggta agaaatattt
    28021 acgattcggc tgggtggact ttatagatga cgaggaagtt gttgtggctc tctttaaggc
    28081 gctcgaggat tggggcaaag agctgggagt cgaagctatt catgggccgc tgggttttac
    28141 tgatctggat cgtgagggaa tgttgatcga aggctttcaa gagattggaa cgatggcaac
    28201 catttataat tatccttact acccgaaata tcttgagcag ttaggttacc aaaaagatat
    28261 cgattgggtc gaatacgata tcactgtacc aaaggttatg ccccagaaac tcgttaatgt
    28321 ttcccagatt gtcaaacagc gacttgggtt acatactttg cagatcaaga aaatcaagga
    28381 tgttttacct tatgcccatc aaattttcga ggtcataaat gcgacttatg ccgttctgta
    28441 cggctttgta ccgcttaccg aaaaacaaat tgatacctat gtcaagcaat atattagcct
    28501 gatgaacccc gattttattg ccattgtgct cgacaaaaac aatcaaatcg ttggaatcgg
    28561 gctcacaatg ccatctttag cccgtgctct tcagaaagcc agaggcaagc tttttccatt
    28621 tggatttgtg cacctgttat gggctttgag acatgctaaa gtagccgatc tgttgatagt
    28681 tggcgttttg cctgaatatc aaggccaggg agtgccggct ttaatgattg cggaaatagg
    28741 tatgaaatta attcgtaaag gcatacagag agccgaatcg aaccccgagc tggagactaa
    28801 tatcaaagtc caatctcagt ggaaatattt cgatttgcgt caacataagc ggcggcgttg
    28861 ttatattaaa catgtctaaa ccctctcaag taaatctaag acgctaaaat tgtttgccaa
    28921 gaaaactgat atcggaaatc agccggttag aagtctgcag aatgtacggc atattattct
    28981 ggatttagac ggcacaattt ataatgggca cactcttttc ccatttatta aagattttct
    29041 cgatgattta aaaaatctgc aagttgatta tactttttta actaataatt cttctctgag
    29101 cgtgacggct tatatccgca aattacgtcg aatgggcttg ccggttaagc caacgcaagt
    29161 ctatacttct gctcaggcaa cgattgatta tttgcggcgt cgtcacccgg ctatcaagcg
    29221 catatactta gtcgggaccc agagcctgag acgtgaattt atagatagcg ggtttacagt
    29281 ggttggcgac gaccctacga acgaaccgga tgccgtaatc gttgggtttg ataccagtct
    29341 aacgtttcaa aaactgtgtg gcgcggctta ctggataaaa accggcaaac tcttcatcgc
    29401 tactcatccc gatctaactt gtcctactga tcaggatacg gtcctggtgg attgcggtgc
    29461 agtttgtgcg gcattgaaag cggctaccgg caagaagcca tcggcagtgc tcggcaaacc
    29521 tgacccgtca atgataaaag gcattatgca aaggttcgga ttaaaaaagg atgaagttgc
    29581 cattgttggc gatcgcctat atactgatat taaaatggca cgacaggcag gaatcgccag
    29641 tgtattggta ttatccggag aaacaacgca ggctgatcta caagaaagtc ccattttacc
    29701 agactatgtt ctcgaaaatg tcggtgaatt gtccaaaatt ctaaggcaaa gtcgcaagta
    29761 gaagaggtgt tccaatagca ggaattgcta ttaatgtaac attgattttg gtcagggggc
    29821 accttttttt agataaaata gacaaatggc attaatatag attttcagat caatgataac
    29881 ttttgattta tttttctgaa agaattggcg ggatcgacgg gactcgaacc cgcaaccccc
    29941 ggcgtgacag gccggtgctc taaccagttg aactacgacc ccttttaaat agcgatggca
    30001 aatttaagta attttccaag tcaggacaag catatttaag tcatttacag tataagtatc
    30061 aagatataga atccaatttt ttaagataaa taggattaac tcagagcaat tttttggaat
    30121 tcaaaggttt gttgagcaca tttaagcaag ctttagacta ctcgtatcaa cgcccccgta
    30181 tcgagaccag gtgaagtcac tgataataat gttcgtttat ccattttaca gtactgttca
    30241 acccctgcgg ggttgcgtgg tatttcgtgt ttttaggact caggatataa tcctgagtta
    30301 ttcgggttca actcctgacg gagttggagg ttgttcaggg ttattcgggt tcaacccctg
    30361 cggggttgga ggctgttcag ggtagctttt aggactcagg atataattct gagttatttt
    30421 taaaattatg tttgttatag atttttttct atattcgtta gtgatgaaat tagacggtca
    30481 tcaggagcaa taaatgggtt ttaaatgcgg aattatcggt ttgccgaacg ttggtaaatc
    30541 aacattattc aatgccctga cgcatgccgg cgtcgccatg gagaattacc cattctgtac
    30601 gatcaatccg catatcggag ttgtaaatgt tccggataag cgactcgatt tgcttgccca
    30661 gatttataag ccggacaaga tcattccgac tacgttgcaa ttcgtggaca tcgccgggtt
    30721 ggtgcgtggc gcttcccaag gcgaaggatt gggtaaccaa tttctgagtc atattcaagc
    30781 agtggacgcc attatccacg ttgtgcgatg ctttgaaaat cccaatgtag cccacattga
    30841 tgaaaaactt gatccgcaac gcgacatcga aatcgtcgaa accgaaattt tattaaaaga
    30901 cctcgaaatt gtggaaaacc gccgggaaaa attaattgcc cgagccaaaa gcggcgatga
    30961 gcaaacccaa aaacaattaa aaattctgga tgatatccgt agtctgctta tcaaagggca
    31021 gtctgccaag caatatcatt ctcacccaga agaagaatat ttcatacgtg atttggcact
    31081 tttgtccagc aagccggtca tcatcctggg aaatatttcc gaagatgaag ctatatcggg
    31141 gaataaatcg gctattacag aagcatttga gaaatttgct ttgcaaaccg gtaatctctt
    31201 cctgaccgtc tctgccaatc tggaactgga attgtctgaa ctgccgcctg atgatcggaa
    31261 acttctgatg aatgaatgga aaattaccga accgggcttg gatcgcttaa ttcgtgccgg
    31321 ctatgaattg ctccgcctca ttactttttt cactatggag tcaaaaatcg tccaagcctg
    31381 gacaattccg gtcaatactc cagccgtcaa agccgccgcc gaaattcata ccagctttga
    31441 agaccggttt atcaaggctg aagtgcgcca ttggaaagat atcgaaagtc tgcgcagtga
    31501 aaatcttctc cgcgaacagg gattagtgca tattgaaggc aagtcctacc tggttcacga
    31561 cggcgatgtc attaccttta aactcgccgc cggctaatat ggcttcgctg gtttctcaat
    31621 tcgataaact caatagtcag tataatttaa tcgccgacaa tcaaaaaatc ctcattgccg
    31681 tatccggagg gattgatagt ctggttctaa gccgcctttt attcgaatta cgcctggctg
    31741 agtttaaaaa tctggaatta ttggcggctt atgttcgcca gaagtcagtg gcgttgatca
    31801 atgaagcact caaagaaatt gccgatctcc tagccgactg gcaaatacca ttttcgatat
    31861 tagatagtga attgccagaa tcgtctcaaa tcaattgcta cgcttgcgct cgcgaaagac
    31921 gaaaacgact attccagttt gcgataatgc acgattgtaa cgccatagct tttgggcaca
    31981 atctcgacga ttatctcgaa accggtttaa tgaacctggt attcaaaggg aatctcgaat
    32041 cgctccaacc ggttcagatc caactgggcg ggcggatacg catcgttcgg cctctgctta
    32101 attggcccaa gaaacatatt agaacaagcg gacgagctta tggaatcaaa tccatcgcac
    32161 ccgtttgccc tatggcaagt ttcaataaac gtcaagatat aaatgcaatg attacaagtt
    32221 ttagtcgcct gaatcgagat tttcggagca atttacgacg cgccataaac cactggaatc
    32281 agctctcaat ctgaaagtgc cttcttgatg tagttaggct ttaggcggac tttcgatttt
    32341 gctttattat tcggacaaga gtttataccg atcacctcgg tctatccgga tggcgagtga
    32401 ttaccaaata attggattct cagactaatt taagccgacc ggcggtatag ttcattttgg
    32461 cgaatatact cagccacggc gggcggaaca aattgatcta tctgtccgcc attcatcgct
    32521 atttggcgaa tatcggaaga ggaaaatggt attaggggcg cattgaaaaa taggacttta
    32581 gccagatatt ccggtttgac tagacttaga tcgcaaccgg ggcggcgggc aacgacgacc
    32641 tgaacttctt gtaaaatatc ttccggttgc cgccatttat gaaattctat caaactatcg
    32701 gcgcccacca cgaaaaataa atcttcacgc tcgagacgca tttggtgttt aatagcccgg
    32761 atcgtatcaa ccgaataaga tatcccgccg cgttcgattt caattgtgga aatctcgcaa
    32821 gatggttcgt tttgtaaagc caacttcagc atcataagcc gatgcaccgg cgaggtgtga
    32881 atttctgagg ttttagtagg cgagatgtta gccgggataa aaatcaattt gtttagagag
    32941 aaagctcgga tagcggctcg ggcaatttct aaatgaccgt tatgcggcgg gtcgaacgtt
    33001 ccgccgaata agccaacttt caatctgctc tcttgcgttt ttctctctct attttacgaa
    33061 gttctttgtg ataagaagtg cgcagatatt caagagcatt cgggtcggtt tggggggtag
    33121 agcgggcaag aatttcctta aaaagaagtt cgtactgatc gaattgacgt tgacgaatca
    33181 ggcaataggc tttctccagt cgcgcttttt cagcccattc ggtatcataa tatgattcca
    33241 acactttatc gaggtaaata atcgctgctt tccattcctg cattttgcga tagagttttc
    33301 cactttcaaa aagcttgcgg gctaatttcg agcgaatctc ggcaattttt tgttctgaat
    33361 cggcgcggaa acgactttgg ggataggtat caataaagtc ctgaaaactt tgcaaggcat
    33421 aaatggaata ttcttgatca agctggtacc gcggggaaga tttgtattgt gaaagagcga
    33481 ttttataaaa ggcttcctcg acatattcgc tgtaagcata gcggttaatc agttgctgat
    33541 attccgaagc cgccaggaga tattctttac gcttgaaata acattcggca atataaaact
    33601 gagcgtcgtc cgccagttcg ctaccggggg aattgataac taccaaagta aaatactcga
    33661 tcgccttgtc ccattttttc ttattaaaat attccgttcc ttggcgataa tgttgctcga
    33721 aatctacaac aggtttgggt ttgttagagg cacagttgtt taaataaata atcagcaggc
    33781 taatcaggac aatttttagg atttgacgtt tgggaggcat ttcaatccgg cacagtttcg
    33841 ggtaggtttg atcggtaaat tattttagaa agccactgaa agagacaaac cacgaattgg
    33901 cattttatag gtacaatcag tcaccaagcc gacatgccag ctgacatctt tctgttctgg
    33961 cggtcggtaa tttttagcgg cgatttgagc atgaaaggcg ctgatcaggt ggttgaacat
    34021 aatcacggtc gtaaaattgg ttgccatttt gagcgattgg ttggattcat agcgttggtt
    34081 gagatacttt ttacgaagag ggctggtagg aatattaaga gaatcaacct cagagacaat
    34141 ttgttcaaaa tcactccaac cggaagcaaa ctggtcgtac ttaccgatat tttcgtaata
    34201 atcgcgggtt ttgattggtc tgagggtccc ttcttgtaaa tatgtttgcc aaccagggag
    34261 ttcacgatcc agataagaaa catcggcttt gtattcttta ccctcatatt cgaaatagat
    34321 agtatgggaa ccaaaaccgc taccggaatc ggaaagtcca aagtattcgg ggtaggagag
    34381 actattttca taccattgcc aggcatccca gtaagtattt gcccagttca tgtatttacg
    34441 ttctaatttt tccccattat tcttgcggtt gaaatagccg acccaggaaa cgacttctac
    34501 gctgaaaaaa gccgccgccc ggtacttggc tccggcataa aattcgcccg cgcccggtac
    34561 gaccgccgat aaaaagaaag ccagcccggg tgatttctca taattaaaca cgaaagtatc
    34621 ctcatcagcc tgggcatatt tcatactata gattaaacgg tcacgctcgg tcaccgtcgt
    34681 gcgttcatcg aaaaacgact gaccgaatcc catgatgggg attagaattg cgcaaagtag
    34741 tgttgcggct aaaaatttcg acttcatgta gcctccctta taagccaaac aatagcgtaa
    34801 aataagttcg ccattcatgt ccgtattggt aagttttaga attggaaacc acctcaaaat
    34861 catccagccc atagacaaag tccaggctga ttgaggtggg ataggaataa aacgaaaaac
    34921 cactcatacg cacttggaaa ccaatatctc ttttaaaatc attcacaata ttacgtagca
    34981 ctgaaacagg ttgggtctta aattgttccc atttcaatcc cggaaaaccg gttacctgat
    35041 tccaggcatt accagcctca atataagtcg ctacgaaggc attttgcaaa ttgaatgtca
    35101 ggatttgaaa atgtttctca cgaaaaagtg gcagacgcca agtataatgc caactgctta
    35161 gattgcgccc ttcgatactg taaaatggat aacctctcaa tcctggttcg ccaccagcaa
    35221 aaaaatagaa aaatgaatca acatccggct tagaaatcca gcccaaatgc catctcaaag
    35281 tgccaaccaa atttttaaat aagggatatt tgtgatacca attgccttca tgttccaatc
    35341 gccagtaata attattgcga aaatctactt gaatagtgct gtaactgcta ttgactttaa
    35401 aaccttgtat aaatttgtca taattgcgtg caatataagt gtccagcaga atgccgttat
    35461 ccggattagt ttcctcattc actttcggta acatcatccg catctgccaa tgcagttgaa
    35521 aattaacgcc gcgatagtaa tcataggaaa agccattttg ataaatatcg gcggctgtga
    35581 tggtttcgtc actactggtg cggtaacgcg accatttaac atcgaaacga acattattga
    35641 aaccgccaaa agggcgggaa atgccgccat aagcttcggt caaataaaaa gtatagtccg
    35701 cttcgactgg ccagccttta tagattgact tctcgttaaa gatattgcga ctgatattgt
    35761 aaaattccaa gaagatagtc ggctcccatt ggtgatattc cagaatcaga aaaagatcgc
    35821 ggtctttgat tctattaatc gaagcccccg caaaaatatt gaagcggttg attatctcat
    35881 ttgacaggca gtagaatccc ggcttgaccg ttccgtaatc aatcatcaag cgcggcataa
    35941 tgaacatttt ggcaaattga tcgtgatatt tctgcggttc tggaatggac accggttgaa
    36001 tctccgaaac cggtggaata ttttgatcat agtctaaata aactgccaac tgcggttcaa
    36061 tcggttcggg gtttttaata caagcaattt taaatgcccg atcgtccaga atcgcataaa
    36121 ccaattcgcc tttctcattg accgctggca taaaagcccc gccggtaacg tttgtcagta
    36181 attgccgttc gccggtagtt agtgattgcc ggtaaatatt gtaaatcccg gtctcatctg
    36241 aggcaaagta caaccacttg ccatccgggc tgaaataggg attgcgggta tcataagccg
    36301 cctcatccaa aatctgcatc gctccggttt ctacatcaac caaagccaga ttacggccat
    36361 gaccaatcag atagtcgaaa gcaatcgtgc gcccgtccgg tgaccagtgc aaggtaaaaa
    36421 cctctactcc ttcactgaaa gtagtaattt ctctgatttt attagagtcc agattcatta
    36481 gggctacatt gctggtgcca tctcgaccag tcacaaaagc tattatttcc ccatctggcg
    36541 aaagggttgg attatagacc cgagcgctct tggttagcgg tttttcctta cgtttcttta
    36601 aatcatagcg ataaatatca aaaaatactg agccgtgccg attcggttta ctcgcccggg
    36661 aataatatag gttttgtccg tcgggtgcga ccgttactct cgagttaatt cgaggaacta
    36721 gcatccggct ttttttttga tggaaatcat atacatataa ggcagtctgg gaataataat
    36781 ctctaccttt attggacata aaataaagca gtgaatcacc gctccattcc ggtcctaact
    36841 gcatcgtacc ctcagccaga ataatttcgc cgactttttc gttagcccgg ataatttctg
    36901 tttgtttgaa atatctctgt tccaattcgg tttgccattg ttggtaaatc tcatcagccg
    36961 cgacaccagt gacgtttttc agagccgtct cacctgacat ccgcactggc ttggaaagtt
    37021 cctctgccag gcgcggaata gtctcagttc cgtaatgttc tgccagataa cgggtgaagg
    37081 caaagccttg attatagacc gattcgttgc cggtgccacg tttaccgaac gaacccattt
    37141 ctgacaaaga caacaatgat ttatgcagaa ctcgatcgcg cagaagcatg tcacggtggg
    37201 tgtcccagaa atcattagaa tttccaggga acatatattg cgccatacct tccgccagcc
    37261 acatcgggat taccataccg gaaattggat aagaggcaat gatccgagga tagccataaa
    37321 ccacatcttc gcgcttctcc ggttcatcat ccagaacttg aatataaatt gccggaattg
    37381 agcgtccgaa tttcatagcc ttttgcaagg aaacaatgtg ggtaaattcg tgggtgatga
    37441 cgtcgcgcaa ccagtcgtga ttgccccgaa aaggaaaatc gagcggtgta gcccagattt
    37501 cgattttatt atcgtaataa tatgaggcgc cattcgaaat atcatcggta tctcggatta
    37561 ccagagcggt tttttcactt ggttcaaatt gataaaattc agttatcggg ccataaatag
    37621 tttcggcaac ttcagccgtt cgtcttgcag tccactccgc cccttgatga taatggattt
    37681 tgaaatgagt ggtctcgaag gataaccact ccagttcagg atgattataa gtcccgaact
    37741 gtgccccggc tggattaaaa aaaaagagca ttattagaaa aatgaccagc gtattccggc
    37801 aaatctggcg ctcagacatt attttaccac cagaatttta attaccttag aataggtccg
    37861 cgaactggat ttggcttgaa ctacggcgaa ataaactcca ctttcgacat tactgacgtc
    37921 ccattccttt tcatttggct cgccgattaa aggcgcggaa tccttgaaat cggcaatcag
    37981 atcgccagcc aaatcgtaaa tattaatcga aatattacaa ctgctattag ggaagtaacg
    38041 aatgtatgta cgattatctt taggcggatt gggccagcaa aaagtcctgg cattatcgag
    38101 caatgacttg gactgaattg gactgctctg ggattgcacc ggataataga attggcgagc
    38161 ggcattccca ccgtaacaaa tccaatcttc agaatcatgt gaaaaagagg agagccgcac
    38221 agcctttatg caattgtaat tttgaggatt tgattcggtt gacgtcagtg ctaaataagt
    38281 tatgccgagt ggtgtattta ataatatgcc agacttttgg ctctgctggt aggcaatttt
    38341 ggggaaaccg ctcaataaat ctcctttata attgaacgcc aacaaagctg aagacatatt
    38401 gataatgaca tccttttttc cgtcattatc taaatcggct gtcaagagtt ccgattcgaa
    38461 ccggtcggaa cctaaaatat tcggaacccc gatcgggaaa ttgtttttaa ctgtcaattc
    38521 ctcgttgaat gccaaaacct ggctggaagt ggcagtgata atttcgattt ttccgtcgtc
    38581 atctaaatcg gcacaggcta aattgtcgtt aaattcctgt tcccggtgta aaacaacctc
    38641 atttggggtg ccgatgtcga gaacaagagt tagcaatgaa tcggcgcaat gaatcaaatc
    38701 cacactttca ttttgattga taaaagctat taccattgtt ccagataagg cgttcggcaa
    38761 atcggctacg acttcggcag aaagtgaatt aagatgaatc ctgattacct gattatcatc
    38821 cgttattccg aagatattat cgtcgtcacc gaccagcatt ttaacctgtg aagcgagctc
    38881 caatgactca atattttgag tatcaatctg atagaggtga aggtaatttt ggttgtcggc
    38941 ataatcttgg gtagcgaata gcacacctcc ctttactggt agaagagggc tgaccaaatc
    39001 ctgagaattc agactgacgg tatcagtttt attaatctgg ccattttgcc caaatttcca
    39061 gatagataaa ctcaaagaag acgcctcggg attattctga agaattccat aaagtaataa
    39121 gctatcttta tccagtgcga tcgctgtttg gcggggcagg gaggctattt gttcgccgaa
    39181 ggaagtatga atccttatgc tgtcagctaa aatcagaaat tcttcaacct ggtcatttgg
    39241 attcaaatct agcggtaata tttcgtcggc tcccgcgctc aggctaagcg gaaaatttgg
    39301 gagcgcatca ttcagtctaa ttcggaaggt tattacttcg ccagctggac cgatgctatc
    39361 aatactaatg ccggtaaaag tataattatt actacaagtt gatggattgg tataaggcgt
    39421 aaagccgaca tgttcttctg catctaaaat ataatccgga ttaagatcaa taaagccatc
    39481 atttccggca aaccagggat cgaaaaacca tccatcctcg atatcttcat tcaaaatatc
    39541 ggcttcatat cccaaatcct gggcaccatc gccttcttcc atatcaacac cccgatgttc
    39601 tcggtcaccg ttaacgctat tagtcgctaa attggcgtta atgaccgatt catcaatatg
    39661 ccagattagt aaaccggagc cttccaatcc ataatcatag tgcggcacag atagcatcac
    39721 gccggtttcc ggtgaaaatt cggcttttag tgagtcacga atcaagggaa aactcagcgg
    39781 caattcaacc atgccgtttt ccatataaat acgatactgg agactatcaa aactgacgcc
    39841 cgggcgaaaa tgagcgcacc gattttctac taaaaaatat tcgttagcat tgattggaat
    39901 cttccaaaca ctcgtatcgg aagcagaacc gactttttta aggacaacat cttcaaaatt
    39961 tttagcgact actggtgttt cccaaccgag aaaaacccgt tcccaggcac atggcaccgc
    40021 tgggactagc ccactcaaat tagccgaacc ctgatccatt aaaccaaatc tgccgatgcc
    40081 ggtcatcccg ctttccgtgt caaataaaca gggcaagcca agataaaaac caaacataaa
    40141 tgccagggtg ccattcaaac cgaattggta atgacagggg gaatcgcttt caccgaatac
    40201 ctcttcccaa ttatcatata gcaagtgatt ctcgctttcc ggcaagatta agccgcaatc
    40261 aacgaaagcc gttccgtttt ctactggaat accctgaaag tcggaatcgt tcggtgctaa
    40321 aaatgccttt agatcatcct tgttcataaa agctgataga atgtcataag gcgtcggatc
    40381 aagcgtaatg gcaaaatcct gaccaactcc ggcatgaaaa actattacga catcgtattg
    40441 cgcaaaatcc acctccggat cggctttctg gactgactca actaaaagtt cagtcaagcg
    40501 taaatccacc aaatcttcga ccaaaaacgg gtgataataa gccatcggat ggtcaacctg
    40561 ataactgtta ttatcattca gcggaaaaac ttccgaatta ttcgtgtcta tcacaacttt
    40621 gccccgggaa acccgattat aataatttgc caaagagcga atatgatccc gaaaataagc
    40681 ccgattgtga ggcgccggat caactacgaa ttcgtcatca cagggagtct ctacggcgga
    40741 cattaggaat tgcccagtgc cggtggtggt ttcgtcttca tcagccggga agctgacgcg
    40801 cagggcgcaa atgcgaatag ctgcaggcgc agtatttttc acaaccgatg gagcagtcgc
    40861 aatccgatac tggggttcaa acctaccgta tgcggcttta ttagctaaaa caattagtgt
    40921 tatcccggta atcaaatatt tatatatttt catcgtgtta ggtttttagc cttttttaaa
    40981 tagggtaaga actgttctga ttatctaaaa ttaaaatttt atattcatcg agaataacat
    41041 tgtattttga cggggatgtc cgggatcacc ggcggtatag ccaaaatcga atcccatacc
    41101 cgcccagcgt aaaccggcac caaaagttgg aaccggatat ttacctttca ttgtgatatt
    41161 gccaggctta tcatacatac aaccagcccg cagagcaaac aaattggagt accaatattc
    41221 tattccgaag ttatacgtca atttttcgaa ttctgccgca agcgagccgt cattggtatt
    41281 accatcctta tccgcagaac cgatgccatc cgtggcggaa tcatacatta ataaccaatc
    41341 gtcaaaccag gaagtaaaaa tccctgtata ccactcatct gaatgggcat attcacgctg
    41401 acccttttta ttatattcac caccaggcac agcatgacca ttttcatcat agccaccaac
    41461 tttttcatcg ccgtcaatat cccgatccgg ataagcggct accaatattt tgttaatatc
    41521 aaatgcaatt gtgactttgt tgtattcatt acctaacctg tacgccagac caagtttcat
    41581 attagtaggc atcgggtcgg cctgattaac atcaatgaag gcaattttag gcccgatgtt
    41641 agccatattg attcccagag taagattttt ggttagaaaa ctatgtttta aataagcgat
    41701 atcgaagcag aaatttgttg agtagccttt tcctaattct tttccggcac cttggtcggt
    41761 caggtgctgg tgaaccattt tcacacccac tccgatcgaa gacgtgcgcg taagagcggt
    41821 cccatacgaa cctgttacag ccagcataaa ggagttgaag gtgccgagat catgtccgaa
    41881 ttcatccgta cgttgctgtt caccgagatt caacagtata atgtttccgc ctactgtgcc
    41941 aacatttctg ataaattgac gatatccgat aaattcataa taaatatcac taactaagtt
    42001 cggtaaccag ttgacatggg tcaatgccaa ttcccgaccg cgcaggaatc ccaatcccgc
    42061 cggattccag taactggcat aggcatcgtc ggcaagagcc gctcccactt cacccattcc
    42121 agaaaagcgt gcccctggat taatcaacaa ccaaacggca ccggcttcgc cttgagccaa
    42181 ggcgcccgcc gtcattaatg atataatccc gagcatggcg attattcttt tcatcaaaac
    42241 ctcctaagaa caaaaaaccg aacatcaata ctttcggtct gaataatata cacaaacgag
    42301 taattttaac gaaggatatt tatctattga cttcgaaata tgggattagc aaaaaggtgc
    42361 gaatttactc ctgataaatt tttaaaattt ttcggcttct tcgattaaca taatcggtat
    42421 atcgtcttta atggcatatt tcaaacgaca aacgttgcaa attaattgct ggtttgaaat
    42481 atcgtactca agttcgccct tgcattgcgg gcagactaaa atttcaagca attcattaga
    42541 taacatatat ttctcgaaaa tttttcattg ttttcaacga cgacagtttt atcaaactca
    42601 ccaatactgt caattgttcc ttttttatat acaattagtt tcccccaaat ttattaatat
    42661 gctctttaaa ttcatccaaa tccttaaatt ttttatagac cgatgcaaat cgaatgtatg
    42721 ccacctcatc caattcctta agctttttca tcactaattc accgatctga cttgaaggca
    42781 cttcgctacc ggaagtttct tcgatttcct tttcaatgtc aattaccaga gtttctagtt
    42841 tctccgctgg aattggccgc ttgttacagg ctattttaat actatttaaa agcttattgc
    42901 gatcatattc ttcacggcga ccatcatttt taatgatttg aagcggatat tctacgacat
    42961 attcataagt cgtatagcga tgcccacagt caatacattc acgccgccgc ctaatggcat
    43021 tgtttttttg cacggtgcga gaatcaatca ctttactttc cggagaactg caaaaaggac
    43081 aacgcataat tcacctataa ttttatctct tgatagagtg ggaaattgcc acatagttcg
    43141 ccaacctctg aacgaacttt ctggtgaata tcttcggaat caggattact caaaacgcgg
    43201 tcaatcaatc cggcgatggt gcgcatctcc gtttcgcaca ttccccgtgt ggtcagcgcc
    43261 gcggtaccga tgcggatacc gctggtgaca gacgggctct tctgatcgaa gggcaccata
    43321 tttttattgg tggtgattcc ggattcttcc agaatattct cagcctgttt accggtaata
    43381 tttttattgc tcaggtcaat caatatcaag tgattatcag ttccacccga aaccagattg
    43441 taatcgcgat gaagaagttc ttccgccagg acttgagcgt tgacgataat ttgttgggca
    43501 taagctttga attccggacg gagagcttcg ccgaacgcca cggctttagc cgcaataatg
    43561 tgcatcagcg gtccgccctg aatgccaggc ataacgttgg aatcaatgat ttccgacatc
    43621 attttggtgc gtccgctttt gggagctacc agaccgaatg gattttccca gtctttgccc
    43681 attaagatca agccgccgcg cggtccccgc agagttttgt gagtcgtcga agtaacaacg
    43741 tggcaatatg gcaagggatc gggatgtaat cccgcggcga tcagaccggc tggatgagcg
    43801 acatctgcta ccagaaaagc gcccacacta tcagcgattt ctctaaactt ggcaaaatcc
    43861 agaaatcgag gataagcgct tcccccggcg ataatcatct tcgggcgata ctcaagagct
    43921 aattccgcca gacgatcaaa atcaatgcga ccggtgtctt tttttactcc ataggaaatg
    43981 attctgaaaa gtttcccgga aaaatttacg ggactgccgt gcgttaaatg tccgccatga
    44041 gctaagtcca ttcccagaac ggtatcaccg acatttacca gcgaaaaata tacagccata
    44101 ttagcctgcg aaccggaatg cggttgaaca ttggcataat cagccccgaa gagtttttta
    44161 actcgatcac gagctaaatt ctcggcttgg tccaccgccg cgcatccgcc ataatagcgt
    44221 tttcccggat agccttcggc atatttattg gtcaaaactg agccggcggc ttccagaacg
    44281 gctggactga caaagttctc cgatgcgatc atttccagag ttttccgttc gcgctcgatt
    44341 tcggctttaa tcgtatctaa aagaacggca tcctgtttag ccaggaaatt gtattccaag
    44401 ttatcaactc tccaatttca taattttttc aacacggcgt aagtggcgta aatcatcgga
    44461 agatggcgtt tctaaaaaag tcttcaatat ttctttagct gattcaactg tggtaaaatc
    44521 agccccgaaa cagagcacat tggcgtggtt atgcaggcga ctaagtcgag cgatttcagg
    44581 ttggtaagcc attacggctc ggataccgtg aattttatta gccgaaatac acataccggc
    44641 gccagtccga caaataagca caccaaattg ggcgcggcca gagacgattg cctcaccaac
    44701 cgctcgggca taatccggat aatcggcact gtcgtcgctg ttgacgccga aatcaatcac
    44761 ttgatagcct tccgaacgcg caaattcgat taatgcgttt ttcaggcgat agccaccatg
    44821 atccgctccg aatgcaatta ccatatttag ttcttcttcg gttcgaccgt gatccattta
    44881 tattcggggc taagaggtcg gaagctggta cgcattgccc cgttagcatc ataacggtta
    44941 aagaaccatt cttcaggagt tggaatcaga tattctttaa ggtggtcaat tcgcgatttg
    45001 acgctggcgt cgcctaattc cattactttt aaataatcgt cataggctaa tttatagact
    45061 aatttatcct caaaagttat ttgacgtccg ctcgtataat aatcagccgc ggcataatag
    45121 aggtcggctc taattttgta agcttggggc gaattgggtg caattgtgat ggctttttgg
    45181 gcatattcca ttcctttagg gaaatcaccc aataattgac agcatcgggc ggcttgttgg
    45241 agaatattta aatcattagg agaaatattc cttaagattt gatcataggc tttcaaggct
    45301 tcagcataat tatttttctg agtgaaataa atatcagcga tattgctcca ggcttcaata
    45361 tttttagcgt ctaattcggc aaccttttga tatacttcga gggctttatc gtattcgcgc
    45421 tgttgtttaa gagcggcggc atattccaag ccatagcgga tattggtagg attcttttcc
    45481 cattgttctt tctgaattgt agtaatgtct ctaccgagct tctggtaggc aaaaatgcgg
    45541 tcgttaaggg cttgctcatc attaggattg atctttaaaa ttccgttagt agcatagatg
    45601 acgtcttcgt agcgctgggc actgatatac agtttgacta aagtgcgata aagctcaata
    45661 tcctccggcg ctatttcgat cattttttcg tattcccgaa tctctaagtc aattttaccc
    45721 tgcaggtggt agatataagc gatatttttc tttaagaaaa ggtcatttgg caaatatttt
    45781 tcacctgcta aataaacata tagggcgcta tcgagataaa tgctattttc actggctaat
    45841 tgctggtaag ctctccccca aaagggaaaa agatccttag catattcttc ttcacaacca
    45901 aaactgctca ttttcttata attttcaatt gcgcttctcc agttttgatt ttgataatag
    45961 ctgtaagcaa aactcaaata caggtcgcac ttatcccgat caatggtcgg cttggtttca
    46021 acttgctcca taaccgattc gctcgattcc ggtgaaggag gagtgcagtt cattaatccg
    46081 atgataaggc aaacgattag tgcttcctca ataaaatgaa tccatcgttt catataatca
    46141 cctcatttat tttattgctt tttatatctt ctaaaccagg gttcaccggt tgtaatcccc
    46201 aggtgaatcc ccagtatttt ttcattgccg atttcgtctg ataaaaagcc attcttcaag
    46261 ccgtaagtta atgcgaaatc tattcgattc aaattataaa aaaatggtaa actgatccca
    46321 gccgtgacgc catactcata aatcccagaa ttttgataga ccggttcggt ttgataataa
    46381 gccccacagc ggtaatataa tttctgtaaa aaggcgctct tcaattcctg acgtggtgat
    46441 ctttctaagc cgattccgac agcataagaa ttacgactgt cgcttttaaa ctgttgaaat
    46501 atgtagaaat tttggaatag atcttcctca tatataccgt actgcatatc ggcatttaag
    46561 gctagattcc tagcgatcgg caaattaatt ccaaccttta taattgtcgg aaaattaata
    46621 ggatcggatt ttatagtggt gtcgggacca aagttgtttt cgatgattgt ccaccttttt
    46681 aactcgttcc ctttttcgac cgtcgcagcc agattatacc gttgactgcg atcacttaac
    46741 aaaaggccga agctgatgcg gctaccgtca acggctatag tgcgtttata gtaactttct
    46801 gagtcaatat cggaatcaat ctcggtgtga ttacggacca cgtaattacc gaacaaaaaa
    46861 ttagctctca agccaattgc cagatttgga gaaacccggt agccgctacc aaggtataac
    46921 tccgaaattc cccctgacaa ttcaactacg gaattatatt cgaatttatc atcttgaaat
    46981 atcaaagaat cagcaaaact aaccgaagat ttcattctgg ataagggcgc cagaccaatt
    47041 gcgaggccag cacgtttgcc gaccggcaat ttaaaagaaa agcccgaaaa atttactatt
    47101 ctacctttga aatcgaggtc aggaattgag agagccgaag tgtacatctg gccttgtaag
    47161 gaagtactgc cgaatccttg ccagagcgct ggattagatt gattcagact catcgagtct
    47221 ctaacggctg tccctatccg acccattcct aatattcgca cggaattggc tggcgattca
    47281 actccgaaac cgtagttggc ataataagaa atttgcgacc agccatttag actgagaatc
    47341 agtaggaata aaatagtacg acgagatgtt ttcattcttc ttgcatcgtt aatatttcca
    47401 aacgaggccg ccgcgccgga tcgggatctt cagtcggata aaatcccagc atagccgggt
    47461 aaagcccact tggcgaaatc cataaggcta ccccgaaatt actaatattg ccactggtaa
    47521 aaagttgtac cgtactggga attttcaaga tcaatatact atcgctggcc gaaaaagttt
    47581 gtgaagtaac ataggatgaa cccgatggtg aaaacgatcc actttcccac tccgctgagt
    47641 ccaacaaagt aatataaatt gtgtgcgaat ttccgtaatc ttcggttagc tccgaattaa
    47701 catgcaagta aagataagca ccagcgataa ataaattttt atccgttatg agttcctcta
    47761 cctgaaattt aaggaaagta taggcggctt ttccggcact gattagcaag ggctgaggcg
    47821 gtgcggtgaa ggtcttttta aattgcgtaa tagttacatc cccgacacag gcaagaactg
    47881 cactggtagt gtctccatta tcagttatat acgaaattat gatatgggga gaggaattag
    47941 tttctccgga ataaattatt cccagaccgg cagctgaacc gaccgccggt ttaattacga
    48001 tgccataatg ggattgagtt gagtcgcgcc agttggcgat aaaagatgtg gtcaaatcta
    48061 aagtatcagc atccgagtag gtaaaagttt tccaatcggt tagatgataa tcatcaaggc
    48121 tgaaattggt actgttagta ctatcttctg accaatccgc attctcctca ccttcgatag
    48181 aagcgacggt catctccaag gacgggtgtg tacttgtgtc gtttaacagg taattgtctg
    48241 tcattagaac caattcacag gtgagaatat tttcaagtgg aagggtatct agcggtactc
    48301 ctaattcttt gaacaataaa agactatacg ccgtgaggtt gtcatccgtg cctactacta
    48361 aataggagct gtagccgata ttaggttcgg caaaaacggt cgtatccata ataaattcag
    48421 cacttacgct aactgaatca aaactgcata agtttttatc taatggcaga gttggtattt
    48481 tttcggaaca accgaacaac aaaaggcaaa agaatgccgc cacactccgg cgataatcaa
    48541 tttttacata atgtaacttt gaccagacac ggttgaaata tggtttagag ctgttccata
    48601 atattgatga tttcatctgc caatttttgc caatcttcag gggattcacc atttaactcg
    48661 taagaagaga atttcttctt atcctgcttc ataatttcgc tgaaaatagg atctttctga
    48721 tatttttcca gcaaattttc atttttatta atgattaata catgatcaca atagggcata
    48781 gccgcggcta ctacgtctaa tcgcccatct acgctttttg ataaatcaag attgttcaat
    48841 cctatttttt ggtattctgt tactttaaaa ttagaacaat caactttgga atggatgatt
    48901 tgcaggattt taataccttc gcagtactct ttatcaacca aattagactg aataaaaaga
    48961 ggaatgagac tcatttgcca gtcattgcag ataattactt ccggtttcca gcgcaaatag
    49021 ctcagggttt taagcgccgc catggcaaaa tagccaaatc gtacggagtt aagatattca
    49081 tccgagacac attcagtcgg gtagagattt ttatcggaat tattaaaata ataaggatgt
    49141 tccagaaaat acacctgaac tttggtattg gggataaaag ccgatttaac cgaggctttg
    49201 atattttcac cacggtactg gaggtccatt tcacgtaagc gaatcacttc ccgtaaaata
    49261 tagcgtcttt cactaatgaa gttatattta ggcatcatca agcgaaagtc gtatttccgc
    49321 tcgttgaaaa ccattgggat ttccttggaa aaaatcgcca gttgataggt ttcagcaaat
    49381 gggattatct cagaggttaa aaaatatatt ctcttaatca taagtgtttt tatttaatta
    49441 attgtttggc gcgttctgaa tattcactgg tcggatacaa gttaatcaat ttttcaaatt
    49501 catctcttgc tcgtttctgg tcgcccattt gtttaaaaca tagtccaatt ttgtattggg
    49561 catcgtcatc tttatcggta ttagtaaaat tccggacttt ttcaaattca atgatagcct
    49621 gctgatattg tttcatcgaa tagtaacttt ctccgatcca atattgggca ttatcagcca
    49681 gctcattggt cggatcatcg gcgattaatt tgcgaaatag ggcaatggct tcttcaaatt
    49741 tacctttctg gtgcaaactc aaggcgttca aataccgatc tttataggtg atgccgggcg
    49801 aagtaatagc gcgatttgtt gcttggcgag catctgtctg actggctgaa ttgtaatcac
    49861 tgatactatt agacaacgat gctaaacgat tttccagaat aaccagctga gatagaattt
    49921 caaaattgat actatcggcg tagcgtgttc tttgttcgac agtattcaaa cgtttctgca
    49981 tattggcttg ttcagcggaa agaatattga gcctgttttc aaggttagcc atggtcagtt
    50041 gaagcggttc gatcaattta ttgatgtcag tggttagggt agctttagtt atagactctt
    50101 tgtctttggc ggtcagtgaa ttgaccgttt tggttagatt tgcgatttgg gtctttaaat
    50161 cggaaatatc cattgctaat cggtcgagac ttgcggctat ttcggcatta ctgagccttt
    50221 tggagtccga acgagccaaa tcaagattta ttaccattag ggataatgca agaacaaaaa
    50281 gtttaatggc agatggctta aattttaggt tatttttgat tggttgttgc atattattaa
    50341 tgacataaat tgtgacgaaa gctacgaaat ttctcgttaa aaagaaaaat ttattttcca
    50401 atcagctttt gaaaatttaa tattgtttat ctattccaat gttgagcgcc aatcctacca
    50461 gaataaaaca ggtcatcaaa aaagatccac cataactaat aaatggaagt ggtagtccgg
    50521 taactggcat cagtccaaca gtcattgcta catttatgac cacatgtatg ccgattatgc
    50581 tggcaatccc aatcatacaa agtgaacaaa agcgatcttt ttgccgataa gcaatattaa
    50641 tgcaaatcag aatgaaagct aaaaagagta gtaataccac taacaccccg atgaacccat
    50701 attcttctcc aattacagaa aatatgaaat cagtatgctg ttccggtaaa aatttaaggt
    50761 gggtctgtgt tcctcgggct atcccttttc caaaaagacc gccggaaccg attgcaatct
    50821 tggattgaag aacctggtag ccggtacctt gcggatcggc attgacattg aatagtgtta
    50881 aaatcctctg ttgctgataa ggctttaaac tattccataa aaacggagtg gtaaaaccaa
    50941 gcgatagatt tatcaacata accgttaaag aaaccctgag tttttctttg gtcaagtaca
    51001 aaatgataac tacgataatt acccagataa aaaaagtata aaaattaaag gcggttatga
    51061 tacttaaaat cggagcaatg atgataaaaa gatggaataa acgggcgcct gcccagtata
    51121 gcatcggaaa caacatcccg aagtaaataa ttgatgttcc taaatccggt tgtttaagca
    51181 cgacagccat tggtattaaa gagagtagag taggaatcaa cacaaattta aaatccaagt
    51241 tggaagatga tgtgctggct aagaaacgag caatcgccag cataaccaat attttcataa
    51301 actctgatgg ctgaaaaccg gccgaaccaa aagtaatcca gcgggctgtc ccagccaccg
    51361 agctacccag aaaatagggc aacaacagga ggcaaaaacc taacaaatac aaaatataag
    51421 ctgagtcgta aatcaacttt ttctgtaaaa aataaatagt tgcggcaacc agaccactca
    51481 atactaacca gatcaactgt cgtccgataa ttttctcaaa aaaggaacct tccatattta
    51541 atgaggcact ataaattgat agcattccga acaaacacaa taatccaaca attactaaaa
    51601 ttgccaggtt caagttggaa aaggagttta aaaaattgtt tattctttta gaaattgagc
    51661 ggcgcattgt ttgctaagga ctgcctcttt ttttgttggt gaataaaatt aaaaatctgt
    51721 ccagcaatct gagcggcgat tccgccgcct gatttaccat gttccaccaa aactgcagtt
    51781 gcgatggttt gattttgttc ctccatatat ccgatgaacc aggcatgggc atcgccgtgt
    51841 gggttctggg cggtaccggt tttccccaaa actggtacat ctttgaccct tgcggctcgt
    51901 ccggtgccgc ggttaatgac atcagccatt gccttctcaa tgaattccca ggtgtcaaca
    51961 gaaatatttt gaatcgagtc tttggcggct ttaaaaatga ttgtttcatt gcttcctcgg
    52021 tcgatatatt ttaatccaac atgcggttgc accaacttac cacgcgtcgc taaagcggca
    52081 cagaagcgag ccatctgaac tggcgttacc agaatatcac cctgtccgat tacgaggttt
    52141 aatttattgc cgtctgtcca gccatgttcg ccgtatttgc gattcagaaa atctacatcg
    52201 ggcatcactc ccgctgattc gccgtacaaa tcaaccccgg tctcggaccc aaacttaaaa
    52261 tcctttccca tattagccca atcattcaaa tccagccgtc taatcaaatt gtagaaataa
    52321 acattacagg aatggacaat ggcttcgtac atattcattg atccatgtcc tcgctcttgc
    52381 cagcatttaa aaatgcggtg ccccagacga tactgaccat aacatcgcac cgaccaatcg
    52441 ggatcaaccg ctttggtttc cagagcggtc aaaactgcga ttagcttaaa ggtcgagccg
    52501 ggcgagtatg ttccttgagt agctcgatta aacattggta ggtcaggatc gcttatcaat
    52561 gcattccaag agctatttga aattaccccg gcgaataacg ccgggtcgta gtccggttta
    52621 ctgacaatcg ccagtatctc tccattatcg gcgtccaaca ctacaatacc tgctttatat
    52681 ccttcagtca acgactcaat gtagatttgc aggtcaagat ctatagccaa aaacatttca
    52741 tttcccggaa ttggaggaat attatctcga tctactacct gaccgatttc ccgtcctaaa
    52801 gcatccacct gaatgaaatt atacccacga gtgcctttca aaatatgttc atattgacgt
    52861 tcgattccct tccacccaat caagtcacct tgtttatatt ccttatattt ttctcgtatt
    52921 aaatcattgg cagagatttc ccggacatac ccgagtgact gagacatttg aactccggac
    52981 ggataggaac gcaccggttt taccgaataa acaacacccg gcaaatcaag gcggtgttct
    53041 tcaatattag taattatttc gacattaaca tcctcagcta ttttaattgg gacaaactga
    53101 ccccgccagt tattttttat gcgagaattt atttctgcga catcgagccc gaggattttt
    53161 gccagaattg aatccgttcg agccgtatga ctgaattcat aagggataac attgatatca
    53221 tattgtgatt tattgtcaac gattattttt ccattacggt cataaattat acctcgcgga
    53281 gcttcaattg gaataacccg aacccgatta gcttcggcga tacctttata actcgggcct
    53341 ttgataattt gtaaataata taaacgaccg atcaaaacca gaaaaactag cacaaagaaa
    53401 atcgtcaaaa tatttttcct gatgttagta attgagttgt gggaaatata catactttat
    53461 tccagagaaa atggagcgaa atattggatt atgaaataaa aaactcccgt atataaagta
    53521 gaaggtaaaa tatatcgcaa aaatatagtc tgggtcgaaa gctctgctcc cttgaagttt
    53581 atcagataga taattgaaaa atgaattata ataatcagta cctcgatggc ataaaacaaa
    53641 atgggattta aatgactgcg gcgtttctta agcaagccag ctccaaaacc ggcgatggtc
    53701 ttggctaaag cagataaacc caggaatgag cctaatccga gtgcatcagc gattagaccg
    53761 atcaaaaatc catagatttg accagcgact tttccttcac tacggccaat aaacaatacc
    53821 agtattagca gaaaatctgg tcgaatcgcg cggattgtca tccagggccc gagcagtatc
    53881 tggattatta tagtcaccag actgaaagca attatcagga gaatctttcg taacattatt
    53941 tactcaaaac cacaaatact tcttcaatcg aattgatatt cacacttggt cgcacagtcg
    54001 caatctgaaa aagattattg gctgattgat ggacctctac aacttcacca acgattatat
    54061 tgggcgggta aatttcgctg aacccagaag tcaatacttt ttcgccagga tagataatag
    54121 ccgttttcgg tatctcgcgg acttcggcta aaccatttgg tcgccagcgc ataatcccat
    54181 gttcccgcga aatctgaaat tttactccca accgaaaatt cacatccaga aaaatctgca
    54241 ccagggtatt attatcaccc accgcaactg ttttacctac gactccatcg ttagtaatga
    54301 ccgccatatt cggaccaaca ccatgcttac ttccaacatt gatcataatc gaattgtaaa
    54361 gcggtgttgt cccctgactg atcacttccg ccggcaaaat cgtgagtcga gacgtatcgg
    54421 caaaattcat catttcccgc aagcgttgat tttccagata agcctctttc aattgggcat
    54481 taagcaacgc cagccgcaga ttttcttcct ttagttcctt attttcttcg acaagattgg
    54541 acaattcgtt gatccaaacc tttggataat gcaaaaaagc aaatgcgtcg gctagctctc
    54601 cctgagtcgc tctaatttga ggactgttgt tatgagccaa gataaataat gagagaaaaa
    54661 ttaaaaatat cagcagtaaa tattcatact gatcaattat aaaacgaatt attttcagcc
    54721 acatggcatt tagactaata catcccgata tttttccgga tcttcaataa tcataccagc
    54781 ccctttgaca acggagtgta gaggcgtttc ggctacgttg accggcaagt cggtctcgac
    54841 ccgaatgcgc tgatcgagtc cctttaggag cgcgccaccg ccagtcaaaa taataccacg
    54901 atcgagaatg tcagatgaaa gttccggtgg tgtattttct aaagcctgtt taatagcttg
    54961 aataatcaga tcgagtgtgt cttttaggca ttcacggatt tcgactgaag aaacctcgat
    55021 agtcttggga ataccggcta cgagactgcg gcctttgacc tggatagtcg tgtcattgat
    55081 cggcatagcg gagccgacgg agcatttgat atattcggcg gtgcgttcac cgattaacag
    55141 attatgctcg gttttaaaat actgcacgat ggcttcatcc atttcatcac cggctacgcg
    55201 gattgcctgt ttggtgacaa tcccattcag agcgataacg gcgatttcgg tggtaccgcc
    55261 gccaatatcg acaattatat ttccgaccgg tttggatatg tcaatcccga tcccgatggc
    55321 ggccgccaca ggttcatcta ccaaatagat ttcccgcgca ttagcccgat cggctgaatc
    55381 acgcaccgct cgtttttcga cttcagtaat gccgcttggt atacaaataa ccattcgcgg
    55441 acgagcaatc cggctaagat taatcttgcg aatgaaaccg cgaatcatcg catcggtcat
    55501 ttcaaaatcg gctattaccc cgtctttcat tggccgaata gtcataatat cgtggtgggt
    55561 tttgccgagc atatccttgg catccgcgcc caccgccaat acttcaccat cgtgagtcga
    55621 acgagcaacg attgacggct catccaagag gatacctttt cctttcatat agataattgt
    55681 attagcggta cctaagtcaa tagctatatc gccagtaaaa atgttgaata agtttaatcc
    55741 cattttcatt tgctaccctt taatgtttaa aaactcgctt acctgtaaat atcataaata
    55801 atttccgttc gttgcaagcc gtgatacttt cctgatcccg aatactgcca cccggttgaa
    55861 tgaaaccaat tactccttgt tcagccgcaa tatccaccga atcccggaaa ggaaaataag
    55921 cgtcggaagc cattactgta tttttaattg acaaaccggc ttcgccagcc ttgcgaatag
    55981 caattttaac cgaatcaact cgcgacgtct gaccagcgcc aattcccacc gtaccatttg
    56041 catcgcagaa gacaatggca ttggatttca agtgctttac aacgtgccaa gccatacgca
    56101 cagtcggcat aaggcttgga tcgggttggc tttctgtcac aattttccat tgactctcat
    56161 catcctcggc ggcttgaatt tcctgaacca gaaggccttg accataagac cgtaaatcca
    56221 aatcatagtg taacgcttcg gcgtttggca tcagtaatcg caaattttta cgcttgctta
    56281 aaatttctaa tgcttgattc gaatagctag gtgcaataat acattcgagg aaactcttag
    56341 taaattcctc agccagattg gcatcaattt cccgattcac cccgacgata cctccaaagt
    56401 aacttaccgg atcggtctga actgcccgcc gataagcctc cagcgtagtg tctccgattg
    56461 cgaaaccaca cgggttagta tgtttgacta tgacaacagc ggctcgctca gttttgaaag
    56521 tagcataaca tttgtatgcc gccaggcaat caacataatt attgaacgat atttccttac
    56581 cctgtaattg ctggaaaggc tgccagttcg gattattgag tggtgcataa agagccgcac
    56641 tctgatcagg attctcgcca taacgtaaag tctgttgctt atgaaatgcc tgaacaatga
    56701 cttccggcat tttttcagct ccggcgcccg ttaaaaaatt ggagatttct gagtcatagg
    56761 tcatcgtttt caaaaaaaca gccgcggcta atttttgccg atattccaga gtcaattcat
    56821 cctggtcgag ccgctgaaga aattcatcgt attgtccggg atcagaaaga atgacgacat
    56881 tcttaaaatt tttggatgcc gcccgtaaca tactaggtcc gccgatgtca atcatctcga
    56941 tgatttcctc aaaactttta ttgccggaat gataagtttc gctgaacggg tagagattaa
    57001 ccacgactaa ttgaaatggc tcaaccttga cgcgcgccaa atcagcgcgg tgactttcat
    57061 cagtcgcatc cgccaacaat ccggcaaaaa cagccggatg tagagtcttg acccgcccgt
    57121 cgagaatttc cgggaaaccg gttaaatctt caattttttc cactgcgata ccggcagttt
    57181 gcaaagctct ataagttcca ccggtcgaaa atatttgaac gcctttccga gctaaagcct
    57241 gagctaaatt cccaatatta gacttgtcgt aaacactgat aattgcgcgc ttaattttga
    57301 actcactcat ttttcccaaa caacctttcc atttacaaat tttattttat cttcgcacag
    57361 ggcttttacc acttccggat aaaggcgatg ctcgacttcc agaacgcgag cggctaaact
    57421 ttccggcgtg tcgtcaggca aaaccgctac tttttcctgg caaatgacgg accctgtatc
    57481 atatctctca tcaacaaagt gaatcgttac cccgctaaac ttgacaccgg aagcaattac
    57541 tgcctcatgt accttcattc cataaaatcc ttttcccccg aaatttggta aaagtgcggg
    57601 atgaatgttg acaattgccc gtggatattt ccttacaacc gggctcggaa tcaacttcaa
    57661 gtatccagct aaaacgatca gatcaacgtc gtatttttct aacacgttca ataaggcatt
    57721 ggcatactct tccggcgtcg caaattgttt acgattaagg cagaaattcg gaattcccgc
    57781 cgcattcgct atctcaaaaa ccggaggtcg ttcgttatca cttataacgc agacgactcg
    57841 ggcggctatc tcgccctttt gaattttatc aaggatagcg gcaaaattag aaccgcgccc
    57901 ggacgcaaaa acagcaatat ttttcaaaac aaccacattt aaataaacat tcaaatttat
    57961 ctaaatatcg tcgtagaatc aattaagatt tatctgataa aaaataaatc tttaaatcgg
    58021 gcttcagccg gtcattatat ctatcgcaaa ttgactggat caatatcgtc catatcgtcg
    58081 aaatcctgat tttctccgcc cattccccag ataaaagtat aatgggcggt cccaacacca
    58141 gcatggattg accagctcgg tgataatacg acttgtttat tatgcatcac gatatgacgg
    58201 gtagattccg gctcaccgca aagatgaaaa acgaggccat tttttggtag atcaaaatat
    58261 aaataaattt cggtgcgacg ttcgtgagtg tgaggcggca tagtattcca gacactgcct
    58321 tccgccagtt cggtaagtcc cataactatt tgacaggtat caagtactcc cggacgcaaa
    58381 tattgaaaaa tggtacgttt gttacattcg gcgtcactac ctaaatcacg acgattagcc
    58441 tgcgcttggg taaccttacg ggtgggatag gcttgatgag ccggataact aagtagataa
    58501 aactcagccg gcttttgggt tgaatcactt tgaaaaacaa tgtcatgtcg ccccttgcca
    58561 atgtataagg cttccttatg ggctaaatga aatgactctt ctccgacaga tacaataccc
    58621 gcctcaccaa tatttataat cccgatttcg cggcgctcgg taaaatattc cgccgctaat
    58681 tcctttgaac cgctcaacgt cagtggtttg tttgccggta cagccgcacc aacgatcgcg
    58741 cggtcggcat cactgtaaac taaacgcagt tcaccagcct gaaataaatt ttcgaccaac
    58801 atattttgcc gtaattcaga cgttgtcata cgttgaaatc gaattggatc ggctaaatag
    58861 cgaatttcca tattatacct cattaattat tttttactta ctggaacgac ctgtcaaaac
    58921 agcagatcga aaagcttcgg catgtttggc taaagcctgc caatcatcgg cggctatcat
    58981 ctttttattc aagagtgccg ttccgactcc cagacagcaa gctccagacc ggatgaactc
    59041 tgcggcgttt tcgagggtga ctccgccagt cggcaacagt ttgacttgtg gcaaaggacc
    59101 aagaatgtcc tttatatagc ttggcccaag cgctgtagtc ggaaaaacct tgaccagatc
    59161 ggcaccctgt tcccaggcgg tcagaatttc ggttggtgtg aatgctccgg gaatggcagg
    59221 tttgtcgtag cgatgacaca tccgaataac ctccggatta aaaatcggac caacgataaa
    59281 ttgggcgccg tttaaaatcg ccagccgggc ggtctcggca tccagaacac ttcccgcacc
    59341 aatgagaaaa tctttgccgg tctgcgccgc aaaatttttg attatctcca gtgctcccgg
    59401 cgtagtcata gtgatttcca gcgcatttat cccgccctgt ctcaaagcgg aaattatgcc
    59461 cggtaatttt tcaccgctat ctaatcgaat tacggcaacc agtcctgatt caatcaattt
    59521 tgcagtaatt gtctgtcggt ccattctatt cccttattat gatttaaagg tttgaattaa
    59581 tttttattat agaatcattc ccaattttaa actgctgaat attaaagttg tcatacaaat
    59641 ttcgcaagtg tttttattat taactggcta aaaagaactt atttccttta gaaaaataat
    59701 ttatttagcc aaatcggtaa tgatctggag agtatgttcc tcagcgattt gcaggtgggt
    59761 tgtcatagcc tcaaaagcac cctgagagtc tttaacctgg attttttcta aaatcaggtc
    59821 atggtaagtg atggctgaac tctttgcgct gtcaatttgt tgatacacca gactgcgaat
    59881 tttcggcatt agccggaaca ttggttcgac gataatcggc actaattgat ttttggaagc
    59941 gatggcaatg ttgagatgga aatctttatc gatctgaccc tgtttgagat aatcggtgga
    60001 atcacatttt ttcaaatcat tacaattttt ccgcaatttc gtcaagtcgc gagcagtacg
    60061 tcttgtggcg gcaaggctag cgatttgcgg ttcgaggatt ttgcgaattt ccatcacact
    60121 catcagataa tctttgtcaa ggttcagttc taggaagaga ctcatcggtt tgattacatt
    60181 ttcggcttga taatcagtaa cgtatatgcc gctccccttg cgaatgcgca aaagtccgcg
    60241 ggcactcaat ttttggagcg cttcacgtaa ggaagtgcgg ctcacgccaa acattttgca
    60301 tagttctgtt tccgacggta atttttcacc gggcagaatt tttttttgac gaattagctc
    60361 ttcaatatgt tcgacaatct cttgactgag actaagcgga ttaccgatcc gcggaaacag
    60421 attataaatt ggttgacttt tattttttgg cgccattctg ctttgatttt aggcagaata
    60481 tagattattt gcaaggaaat ggatttattt ggaggacatc ccaaattcca tctcatttag
    60541 tataaatagg aagctactac gtggctattc ctctgtagat ttagtggtgt ttttcatcat
    60601 tgtttcggca tgtcagtagt cctgtgttaa tccgtgatag attgtaataa caactcaatt
    60661 tacattttct cttttctcag cctttgcctt attccgactt gctcaaaggg cattttcaat
    60721 gacgcccata attccagaag tggatttcag cggttgcatc aagcagtcag cggataaagt
    60781 taccccaata ttttccgatg gtttaagtat ttcgaaaata accttctgat cagtgagggg
    60841 ccagtcccgg aaaccgggac tgacgcggcg caaagtctgg ctgggagata accaggagga
    60901 cgatttttca agatactgtt gtaaatcact ggtagcggtt tcgaccaggg cggcggctag
    60961 cccgtttaat agaaatgcct gataggcatc gccttccgtg taatttaaat cggcaatccg
    61021 cttatcaatc gccgaaccaa tagttacaat aaatataaag atcggtttag gttttactgg
    61081 gtcggattta ttcacaaaac ggtgaaggct tttgccgtgt aattcatacg taccaccagc
    61141 aagcgccaag ccggtttcgg ttgggaggca gtcaaccaat cgaacgatag cggccggctg
    61201 aatcaatgcc ctattttctt tcaaaagatt agcaagttcc acgcgcatac gaaaggcgac
    61261 actggtattt tttaaaatgt tatatttcag tgcggcggtc aattcccgca agaaatgctg
    61321 aaaaagtact aattgatgat attgttcatt cggcgagatt aattgtttat tattttgacg
    61381 catttggaag atttgggcta aggaaggagt ttcaccgtta tttttttcaa tagaaaacct
    61441 gattgctcct gggtacccgg taatctcttg cataagtgta attaagtcgt cctgtttaac
    61501 caggaaagag caaggtttga aaatcgttat tttagtgaaa tctatcggca ttttttataa
    61561 ggctaagata atccaaaatt tcggataatc cgcgtaagtg tgggtgtgga ttataatcga
    61621 gcgcaatatc atttctgact actatgcctt tggaatttgc ttcgtcaggg acagaattgt
    61681 ttaattttct gcattatttc ttccagtttc tcctgatcgc ggaaaatgtc aaactgatcg
    61741 gtttcgacaa tcaaaatatt actacgcttc tgctcctctt ttatccaacg ttcataagag
    61801 agattcaagg tatagatata ttcgggggaa atgtcacgct cgaagccgcg cgaacgcttt
    61861 ttgatgcgcg tcagaagggt gtcaacgctg gcgcgcaaat agacgatcaa gtccggtttt
    61921 ttcaggtagg aagtcattat ttcgaagaga gcccgataat tgtcccaatc gcgctgactc
    61981 atattgccca tttcgtaaag attacgggcg aagatttcca catcctcgta gattgtgcga
    62041 tcctgaaccg tggtgatgtt gctttcggtc atttgtttat gactgcggaa acgatgggag
    62101 agaaaataaa tctggagatt aaagctccag cgcttcatat cttgataaaa gtcttctaaa
    62161 taaggattgt caataacgga ttcgtaaaaa gcgcgccaac caaaacgcct tgcgatcagg
    62221 tcagtcatag tggttttgcc aacgccaatg ttaccggcaa ttccgatgaa atagggatgt
    62281 tcactcaccg agcaattctc cttctatata atcttcggaa acgtggatta atttaacttt
    62341 ttcgattttg ttcagcaggt tttctcgtgt gtggatgcga acttttaggt aattgtccgt
    62401 caagccctgc cagagtccgg gtgctatttt ttcttcaaat agaacggaga cagtttttgc
    62461 gagcattgat tgcctaaaag cggcccgttt tcggttactt aattggcgca gaatttccga
    62521 gcgccgtttc ttttcggaat cgggaatacg gtcggtaaat ttgccagccg tggttccggg
    62581 acggggcgaa tagcggaaaa cgtgcaggta attaatgtcg agattttcga gaaattggcg
    62641 catctggtca aattccaaat cagtttcgtc gggaaaaccg accatcacat cggcaccgat
    62701 gcagatctct ggtatttgtt gtttaatccg gttgataagt gtggcataat cggcgctgag
    62761 ataacgacga ttcattttgg ctaaaattgg atcggaaccg tgttgcaaag ggatatgcag
    62821 atggcgacaa aggcgcggtt cttcgtaaat cagtttaatt aattccgaag tgattaagtc
    62881 cggctcaatg gaactcagcc ggatacgcgg caaaccggtc gcggcgagaa ggtgccggat
    62941 taaaaaagat agatcgccgc tcggtgattc atagcgacca agattgatac cggtcaggac
    63001 gatttcctga acgccttgat tctcaagaag tcgagctgtg gcaatgcaat cctccggatc
    63061 gcgactgcga ggattgccac gcaaatgggg aattatgcaa taagcgcaat aataatcaca
    63121 cccttcctgg actttcaaat tagctcgaat gcgtttatca ttcggtaagt gcgattcgat
    63181 gcggaaagtt tttggaaatt ccggtttggc aactcgtaca attttttcac cgcgctccat
    63241 cgtttccagg aaatcgaata tttcgtactt ctcattattg ccaagcacta gaaagacgcc
    63301 ggggatatta agcaattcgg cggcggcaat ctgggagtag caaccgataa cggcaatttt
    63361 gccattgggt gaaatgcgtc gtgcggcagt gatggcgccg cgagtcttgg cttcggcggc
    63421 ttgcgtcacg gcgcaagagt taataatagt caaatcggcc ggtttattgg aatttactat
    63481 gtgcagatta cgatgggcaa gttcggcctt aatggcatct gtttccgcct gattcaggcg
    63541 acagccaagc gttcgaaatg cgaccgttgc aatcaagtct gcctgatttc aattatactt
    63601 tgatcgccga taatgaaacg attggtaaac ggtttagctt gtgattcgat gatctggacg
    63661 tttttaccga gtaggctggc ttcgatccga attttttgat tgcggatcac gcagttacag
    63721 gaaacgatac tgaattcaat ctcagcattt ctgatttcgc aatttttatc tattgcggta
    63781 tatggaccga tataggcatt gacgattttt gcaccttcgc cgataatgac cggaccgcgg
    63841 atgttgctat tgataatttc ggcgccttta ctgatgacca cgcgaccgct gagctgacta
    63901 ttttgatcaa ctttgccttg gatagaagtt gtgatattat ccagaactaa acgattggct
    63961 tctaacagat cgctgggttt gccggtatct ttccaccagc cggtaatttc cgaaaaggtg
    64021 actttgtaac ctttatttaa taaatattga tgcgcatcag agatttccag ttcaccgcgc
    64081 gcactcggtt ggatattatt gaccgcctcg aaaatggtgt ggtcataaat ataaattcca
    64141 gtcacggcga aatcgctgag tggcttttgg ggcttttcga caatactgac gatgcggtcg
    64201 ccattgattt ccggtacgcc gaaacgttct gggtcaggaa ctttagctag tacgagatga
    64261 caattggtct ggttacgttc gaattcttcg ataaagcgtc ggataccgcc gatgattata
    64321 ttatcgccga gataaaaaac aaacggttcg ttgccaatga acggctcggc aatccggacg
    64381 caatcggcta aaccggtcgg tcgttcctgg ggaatgtaaa cgattttcag cccaaaggct
    64441 tggccgtcgc cgtaaacttc acggacttcg ttgccttctt tattggtaat tattccgact
    64501 tcctgaattc cggcatcgcg gacatattcg agggcatagt tcagaattgg tttattggca
    64561 atcgggatca ggtgtttatt ctgagtatgg gtgattggtc taaggcgtgt gccgtgtccg
    64621 ccggctgtta tcaatgcttt catcgttcat tcatcctgat tattattaaa ctaaaatctg
    64681 accaaaaatt agctaatttg gatttaacta acaaaagttt taaccgttgg tttcagctcg
    64741 taggtctaag ttttccactc cgataatggt tacttcataa aaatcgccaa ttgatagaat
    64801 tcttggtgat tttactcgaa cattgcagtc aatttccgga gcatcccaga cagagcgcgc
    64861 gatatactca tcaccatctt gctcctcaat cagcgcggta attttctgac cgattttact
    64921 gcgtgcaaag tcctcaccaa tgtcccattg aatttgcatc aatagatcac ggcgtcgctc
    64981 tttttccggt tccggaatat cgtcgttaaa cttttcagcg accgtgctct cttcggcact
    65041 gtaagtaaaa accccgagcc gctcgaaacg tatttcttcg ataaaattca gcaattcttc
    65101 aaattcggct tccgtttctg aaggaaaacc gaccataacc gaggtgcgca gagccacatc
    65161 aggacggtac cggcggatat tccgcaaagc agtcttaagc cttttaggat tggcaccgcg
    65221 gttcatcaac ttcaggaggc ggggactggc atgttggatt ggaatatcaa tatatgggca
    65281 aaactctgga tgttgagcaa gaatatggtt caagtcatcg cgccagaaat ccggattgac
    65341 atacattagt ctcagccagg gaaaaagccg tgccgcaagt aattcttcca gcagagtttt
    65401 taaactaact cttccgggca aatcactacc gtaacgagtg gtgtcctgag caatcagaat
    65461 caattcctta actccctgcg aattaaggta ttcagcttcg cgcaggagtg attcaattga
    65521 ccgactgcgc tggcgtccgc ggatcaatgg gatagcgcag aaagagcagg aattgtcaca
    65581 gccttcggcg attttcaggt aggcgaaatg acgcggtgtc attagctggc gaatagtttc
    65641 cagatcggga ccaatatttt ggctaccggt cagaaatcga atcacctcac tttgcgattc
    65701 gacgccgaat aagccgtcta tctcgggaat ttcagccttg atttgcgcat agtaacgctg
    65761 ggagaggcaa cccatcacga ccactttttt gacgcctgtt tttttgagtc gcagggtttc
    65821 cagaatcgtt tcaatagatt cttctttagc cggttgaatg aaaccgcaag tattgataat
    65881 aacggcatcc gcttccgccg gatcggcttt gaattccatt ccagcctgca ttagaccgcc
    65941 ttttagaatt tcggaatcta cggtattctt cgggcaaccg agagagataa tggcaaaaga
    66001 ccgaacagct ggtattagag acattaatga gctaattttc tgctaaagaa aagtatccaa
    66061 gagaatttta tgagttttta taggcattta aggatgatac tgtgatcaat tttaatctat
    66121 tcattcaatg tttaatagat cgcggatata ctgattcgga tcaaactcta ctaagtcggt
    66181 taatttttca ccaacgccca ggaagcgaat cggaatattc aattcgcgat ggatggcaag
    66241 agcaattccg cctttggcag tgccatccaa tttggtcagg attagcccag ttatcccaat
    66301 ggctttctgg aattctcgcg cttgcaatat tccgttctgc ccgacggtgg catccagtac
    66361 cagccagact tcgtgcgggg catcgggaat taccttacca atgacgcgct tgaccttggc
    66421 taattcttcc ataaggttgg actttgtgtg caagcgaccg gcggtgtcaa tcaggagaat
    66481 atcggcattg cgtgcttgag cggcgcgggc ggcatcgtaa gcaatggctg aagggtcttt
    66541 gccctgtggg ttacctatga attctacccc actcctttca gcccagatcc gcaattgctc
    66601 gtaagccgcc gctcggaaag tgtcggcggc acaaatcaat acgcgctgac cttcgctcgc
    66661 atattttgcg gccaatttgc cgatcgtagt agttttgcca gtgccattga ctccgacgat
    66721 tagaattaca gtcggtttgg caaaattctt ctccgttgtt acggccggct gaattaatgc
    66781 gcttagctcg ttctgcagaa tttcattcag gcgagtagct tcttggcggt caactttgcg
    66841 caggcgttcg attaaatttt cggttaattc tactccaagg tcggtttgga ttagagtttc
    66901 ttcgatttcc tccagcgttt tttcatcaag ccggaaaaac cgccctaata gaggcaggcg
    66961 actggctaga aaacggccgg ttttatttaa accaaaatta atcattttta gaaaggttgg
    67021 tcttcttctc gcggttagcg attataattt gtcgcgaata ttcataaaga tattgaacta
    67081 gggacaatgc cataaaaatt atggaaacaa tcatcaacgg cttagccgat gagccgaaaa
    67141 aattgggata gatgaaagcc agcacggtga ttacagtaaa aacaactgag acttttccca
    67201 gtttatttga ctgtggggaa accttactat tattcaacat ataaattcca aataccgaaa
    67261 taaggaaata acgaacgagc attatggtaa aatagaaagt cgggatacgt tccttgactg
    67321 ttaagaacag cataacgctc ataatcacga cgctatcggc aaatggatcg agaatcttgc
    67381 caagcacagt gatttcatta ctggagcgcg ctaaccagcc gtccaacata tcggacaaaa
    67441 tcgcgatcag gatgaaaata agagcagtct tgctgtcatt attgcgcagg gaatagacaa
    67501 tcggtgccgc taaaaatgct cgaactaaag aaatgatatt ggagatagtt acggttttac
    67561 gggcagggat ggcgtcgtac ttggaggtaa tgttacgttt gacctgaaga gtgtgtttaa
    67621 ttggattttt gctcattgta gatgtccctc tggtataatt tttccaataa attcaacccg
    67681 acttcgtgaa ttatagaatg attttgatat agtgtcttcc attcgccttc aaaaattaca
    67741 gaaccctgat taaaaattaa tacattgcgg cataatttac gaaggagacg gtagtcgtgg
    67801 gaaataatca taaattcttt ttgcggatca tactctttga gaatgttttc aaatttcatc
    67861 cggccagatg ggctaagagc aatcgttggt tcatcgaaaa tggtcaattg aggtgcaagc
    67921 gttattccca gagcaatagt agccaatcgc agttcaccgc tactcaattc gtagccatag
    67981 cgttcgcgaa tgagactata atccagtcct aattttcgga ggttatcggc gaatttaatc
    68041 gtcaaatcgg ccgggtcatt ccagttctta agaattttct gccaggcatt gccgatactc
    68101 attccgagaa aaattaactc gggaaattgc ggtagataaa gaatacgcgg tttgattttt
    68161 ttatctgatg aaaatggttc ggatgtttcg atctgaaaaa atcccttcgt gggaattaat
    68221 aatccgctca ggattttacc gaaagtgctt ttaccagaac cggtcagacc gtaaacacca
    68281 aggggataat tcagagggca agtcaattcg ggaatagata aaaaaaagtc cggatttcgg
    68341 gggtattgga attcgaggtt ttgacagatt aggtaattca tttaattgta aaaataggat
    68401 tatttattcg aactcgtccg gagcaaatag ccaggcaata gctcaattat gtaatcggcg
    68461 aaagcgattt cataatcttc ctgagaaatc cagatgacgc tgagtccatc gacgattaac
    68521 tggcgaatat tttcataaag ttcaaaacga gtcggcaagt cgagaaaagc ggtggtttca
    68581 tcaaaaatca gaacttgcgg tttgcttact aaccaatcag cgattgtcag tttctgcttt
    68641 tcaccgccgg atagttcatg cggtgaattc tcacggcgcg gccatagatt gacactctcc
    68701 aaagcaagtt gcacggcctt ccggacgata gcgggactgg caccgcggtt ctctaaatta
    68761 aaggcaactt ctcgttcaat attgaagtgg acaaactggt catccgggtt ttggaaaata
    68821 tagccgactt ctaatgaatt attgagacca ttatcatttt caaatcggat tgagccttta
    68881 tccggcttga gtaaaccggc gattaattta gccagagtag ttttaccagt gccacagtca
    68941 ccggtcaaaa ctgtccagca atttaaggga attgtcaggt tgagattttt gaatacaggt
    69001 tggtttccgt gataggaaaa atctattccg tcaagtcgaa gtatcattcc gactccagga
    69061 tttcttggac ggttcgggat gaagcgccca tattttttag aatcacctgg cgggcagatt
    69121 gggcggcttt ctggtaatcg tccggtttag ttattagatg tttgatggtt gaataaatac
    69181 ccggttcatc aaggcagacc agtccgccac cggactgcac caataattcg gcttcctggg
    69241 aattatgata cgatggtcca aagagcaccg gcaaaccagc gaccgccggt tccatgacat
    69301 tgtgaattga acctcggaaa ctaccaccaa cgaaagccag atttgcctgg tgataaagtt
    69361 cagccaggat accgatacga tcaataagca caacccgtac attggcaggc atgcccttca
    69421 attgtgaata acggactgtc tcaatattat gcgtctgaaa ttcatcctcc agcatttcca
    69481 aagcatagtc attgggttca tg
//
LOCUS       DAOSMP010000006        67645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_438_length_67645_cov_18.563930, whole genome shotgun sequence.
ACCESSION   DAOSMP010000006 DAOSMP010000000
VERSION     DAOSMP010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 67645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 67645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67645
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_438_length_67645_cov_18.563930"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PKX09_01695"
     CDS             <1..722
                     /locus_tag="PKX09_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOU16575.1"
                     /translation="TQTLGIINVVGSTIAREVDSGIYLHAGPEIGVASTKAFICQVIT
                     LLLLALYIKQQKGELTDPALLAKIDMIPEQIRQVAEHSDNLREIASAYYLNRNFIYLG
                     RGINYPVALEGALKLKEISYIHAEGYPAAEMKHGPIALIDENMPVVFIATKGAVFDKV
                     LSNMQEVKARGGQILTITDHNNPHLQELSNHLIIVPETSEDLSPLINVIPLQLLAYHI
                     ADLKGLNVDKPRNLAKSVTVE"
     gene            856..2190
                     /locus_tag="PKX09_01700"
     CDS             856..2190
                     /locus_tag="PKX09_01700"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOU16576.1"
                     /translation="MLKEISLGVILMFSLWVTGQTAEPFFPYKYNVEVLPNGLKVITI
                     PMESNGIVAYYSVTRTGSRDEWEPGHSGFAHLFEHLMFRGTEKYPGSLYDKTITELGA
                     DANAYTTDDYTCYHLNFAAEDLEKIIEMESDRFINLAYKEEEFKTETGAVYGEFLKGR
                     VSPWFVLYEKLYDVAFDKHTYKHTTIGFEADVKAMPTMYKYSKTFFQRYYRPENVVLL
                     IVGQIETEKTLELVRKYYGDWQPGYVSPQIEPEPPQTAERKAEVIYPGKTLPIVMVAY
                     KSDAYDPNNRLYVAGLLLEQLAFGENSDLYKRLVLKEQKVEFITADFSVSRDPKLNEI
                     YAMVKSPDDIDYVREQIFMTMEQFKTEAVPESRLEDLKKHLKYSFLMNLDTPDNVAGN
                     LANYVAITSGIETVDQLFNQIMNVTVTDIQKAAQYYGVPEKRTVVILKGVAQ"
     gene            2187..3698
                     /locus_tag="PKX09_01705"
     CDS             2187..3698
                     /locus_tag="PKX09_01705"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOU16577.1"
                     /translation="MKTKLNLLLSWGIMASILLSNCGSKIDVSRCVLLPVADDPTISY
                     RIYFKVGSQDDPAGKEGLAFLTAHMLSEAATQSNSYEEIIAKLFPLAASYDATVSAEM
                     TVISGRVHRDNLPAYYPLFTEAILKPAFQEDDLNRLKSNVLNYLENVLKYANDEELGK
                     AVLYNEIFAGTGYGHPTEGLINSINSITINDVKDFYQKMYNRNNVIIGLGGGYDNETL
                     TKLQKDLARLPRGVENIATRAVPYDIQGLKVTIVEKDANATAISLGFPINITRENPEW
                     YALAVANSWFGEHRNSSSHLYQVIRETRGLNYGDYSYIEHFPNSGYYQFPPVNVARHQ
                     QIFEIWIRPVPNETRHFVLRATMRELHNFIENGLTPEQFNLTRNFLRKYILHYAPTTM
                     MRLGYAIDDKFYGIKGSHLENFRVALDNMTVNDVNSAIRKYLQYDNLQIVIVTNEAEK
                     LREDLLANTPSPIKYVTPKPDVVLKEDLEIANYPLAIQPENIKIVNIRDLFYE"
     gene            3691..4662
                     /gene="nadC"
                     /locus_tag="PKX09_01710"
     CDS             3691..4662
                     /gene="nadC"
                     /locus_tag="PKX09_01710"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOU16578.1"
                     /translation="MNNSAKNFTDSRTKDVKQCFDISTELRLDNKYYRKFVLTFFDNL
                     LDFDISSKDASLQPAKYYQRQVTAIIKAKAPAIIAGMAEIQAFLDHHNLLSTSDFKDG
                     DPVKGGETIMTIEGRADRILTVERTVLNIFQRLSGIATVTAEYSQALKGLPCFVTATR
                     KTLWGIVDKSALQYGGGLTHRLGLADAAILKENHLTVLKNSGDPQALMTALDAIVATD
                     PFLRFVEVEVTNDDEFWQVVEAYNRLRPETYCVIMFDHFAPENIAQLIKATKNNGVYP
                     TIFFEASGNITLETIRDYAASGVDVISVGALTHSVKSADFSLLINKF"
     gene            4771..6969
                     /gene="selD"
                     /locus_tag="PKX09_01715"
     CDS             4771..6969
                     /gene="selD"
                     /locus_tag="PKX09_01715"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOU16579.1"
                     /translation="MKPIYLDYNASTPLLPEVVDAMLPFLKNVFGNPSSSHFYGQESK
                     QAIENARRQVAEMLNCQPEEIVFTSGGSESNNFAIRGIAEMHPGGHIITSTIEHPAVL
                     EVCRYLESKNYSVTYLPVNSDGLVELPAVQAAIRPDTFLITIMLANNEIGTIQPIREI
                     AALAHKHGILVHTDAAQAVGKIVVDTRELDVDLLSVAGHKMYAPKGVGALFIRTGTEI
                     APLIYGAGHERGLRPGTENVLEIVGLGKAAEIFRAKSAEIVPRQKALGEKFFNDLRTA
                     IPELGLNGHPTQKLANTFNVYFPGIDANTMLAELTQVAASAGAACHASSITPSHVLKA
                     LGLPNERILGSVRFSLGRFTTEDEIADAVSAISQTYHRLIGAEEISVPITFDEPIRLT
                     QFTHGLGCACKIRPQTLEKILREIRPRMALNVLVDFSASDDAAIYKINDEQAIVATVD
                     FFTPIVDDPYDFGRISAANSLSDVYAMGAEPLFALNVVAFPEKRLPVEILTQILRGAQ
                     VIADQAGIPILGGHTVEDNEPKFGLVAIGIIHPEKIIRNIGAQPGDALILTKPIGVGI
                     LSTALKRGLLDELAKNLIIANMTELNATAAEVMKSFPVHACTDITGFGLLGHLHEMTT
                     GSRVRAQISASSVPILPSTNEMVLRNVIPGGTEANLSFISPMVIWDKMITDSDKIILC
                     DAQTSGGLLIAVADEFANDMLAQLHQAGVINAAKIGKIESGADGKIKVVP"
     gene            6996..7916
                     /locus_tag="PKX09_01720"
     CDS             6996..7916
                     /locus_tag="PKX09_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16580.1"
                     /translation="MTRFRKEYSKIARKLTSADLQSGIGNSELSLDNIVSDGRLAVDS
                     QFFMGVFSRQGLLNAIRAFGLLDILERKGLTNITIDIDTSDPYIHRLYAYSGKPLNEN
                     KICELVLKQGPLHFENKPLSSFPRRTANILYIEWLLLQNPREKFSPEKPPLPGQNYPG
                     LGIGDRLMEIMILMTRRLNLEGIINKPHYFHTAFMFSKEFVFINPYNQALMYAISKDL
                     LAKYSFWMVAWAAHFNCIINKNDGKPMDWKTGDLILPFTKDLIKYFRGREYQQEVSRL
                     IANFSFEIDLDKLHTALKSNDLEKYARFPK"
     gene            7931..8599
                     /locus_tag="PKX09_01725"
     CDS             7931..8599
                     /locus_tag="PKX09_01725"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOU16581.1"
                     /translation="MTPNTPINFQSPTALVLGNGNYPNWFDIKQFQVEYKVLLVCADG
                     GAEKARQLNLIPDLIIGDMDSVTTETINHFQASEVKIIHQPNQNNNDLEKCLRWLLRH
                     GWRQFVLVGFTGRRDDQTIATLQIMRKYARRAQFLLFTETAQIYLLKKGIWLINAIPG
                     QTISLFGFPRVYHLTTRGLQYPTKDENLTGGSHGLSNTAIDEMVQIQFSSGYLLVVAI
                     QELN"
     gene            8596..9984
                     /locus_tag="PKX09_01730"
     CDS             8596..9984
                     /locus_tag="PKX09_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOU16582.1"
                     /translation="MTSLRLAPIDLLIIAIYLVGIFLIGLKYSRNYRGNVDDYLLAGR
                     RLSLPAFVATLVSTWYGGILGVGEFTYRYGLLNWVTQGLPYYFFAILFGIFLAPRIQR
                     SGLYTIPDQLYQRYGKPAGFLGSVFVFLLTNPASHLLMSTLLLSAIFGISFWLAFLIG
                     VIFSIFYVFMGGMKSDVLTDFFQFGLMFLGFGVLAVKLITNYGGLNFLQANLPQNHLR
                     FTGGENWQYIMVWFFIAFWTFVDPGFYQKCYAARTPQIARRGVLISVIFWLLFDMLTT
                     TCGLYARALIPDINPLMAFPKLGEMALGDFWRGIFFIGLLATIMSTLDSVALLSAMTF
                     GRDLLWRLKPNSNINRNTQIGFFVIIGLSILVILFLPSVVKIWYTFGTLFIPALLIPV
                     ICNFTKYNYKSSVMFTGMLGSFFATLIWFIIGMRNGSIMEPIYLFNIQPFFIGLLSWL
                     LFLPFLVDRFTT"
     gene            10100..10173
                     /locus_tag="PKX09_01735"
     tRNA            10100..10173
                     /locus_tag="PKX09_01735"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10134..10136,aa:Val,seq:cac)
     gene            10282..11082
                     /locus_tag="PKX09_01740"
     CDS             10282..11082
                     /locus_tag="PKX09_01740"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOU16583.1"
                     /translation="MFKELERLVEVQKIDIKLLEINNLRGDLPQIVENLQAQIDSLSK
                     ELENERKREQEITREIKELESKIADDRILLNRYQDQLYLVTSNKEYDALTSEIDNLKQ
                     EIDNAEYNILKLSDEIERLKISIKSKELTLEEKTRELDQKQSLLDKTDITTKDAQEAL
                     LEQRKRIVDLIPIRYMREYERIAKAKNGIAIVPILQIFEEKVDKKGNVEYIPMQVSCG
                     GCSKIVPPQKILEIRSGDQINRCEFCGRILYWDEQSSEIRSNYEEELF"
     gene            11081..11468
                     /gene="rnpB"
                     /locus_tag="PKX09_01745"
     ncRNA           11081..11468
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKX09_01745"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            11479..13185
                     /gene="recJ"
                     /locus_tag="PKX09_01750"
     CDS             11479..13185
                     /gene="recJ"
                     /locus_tag="PKX09_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOU16584.1"
                     /translation="MKQKWLISNPNPNAVHQLAGELQIPPAIVRILLNRGLFDKDSIE
                     KFFSPSTQDTHDPFLMADMTKAVERLGQAILQREKVLIFGDYDVDGTTATSLLYLVFH
                     EIGLDSAFYIPDREREGYGLSQLGIEYARQIGATLIITCDCGINAFDTIDYANSNQID
                     VIVTDHHEPAEKLPAALAILDPKREDDHYPFKELSGVGVAFKFLQGFHIANNLPVTNL
                     YRHLDLVAIGTAADIVPILGENRVLVAKGLEYLNSSQKVGLQALLKTSGFDSKTMNVV
                     NIVFGLAPRLNAAGRLGEASRAVHLLTSFDRKEAFELANLLEQENRNRQAIERGTIDS
                     AILQLNATHDLDADKILILSNTNWHHGVIGIVASKLKELYNRPVVMISFQNGIGKGSA
                     RSIPGFDLYSAFNSCADLLENYGGHKMAAGLTILIESLPEFTRRLKKIAAEQIDEQML
                     NSGLQIESEIDFNDINPQTLSFLHKMAPFGPGNMRPVFCTRNVTVSGMPRIISENHLK
                     FKAAQNRSVLSAIGWKMGCYYEMLISNRPLDIAFVIDENEWNGYKEIQLNIKDIVYSD
                     YH"
     gene            13210..14202
                     /locus_tag="PKX09_01755"
     CDS             13210..14202
                     /locus_tag="PKX09_01755"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOU16585.1"
                     /translation="MYKSKIASLGKNVPENIVTNADLEKLMDTSDEWIVERTGIRERR
                     FVTPPCGASQLALPAVLEAITSAGLTKNDIDLIIVATTTPEHLTPGTSSYLQALLELP
                     NIPTLDIRVQCSGFIYGLAIADAFIKTGQYRNILLVGVEVQSVGLNMTTAGRDIAVLF
                     GDGAGAAVITRSSDESAILSTHLFGDGRYANELWVEAPDGSKRPRLSKEMLDEGKHYP
                     KMNGRQVFKHAVTKFPEVINIALEANNLNKSDITLIIPHQANLRITEAVAQRLEIPMS
                     QVYSNIHKYGNTTAASIPLAMYDALQEGRYKKGDYLILAAFGSGFTWASAALRW"
     gene            14231..16618
                     /locus_tag="PKX09_01760"
     CDS             14231..16618
                     /locus_tag="PKX09_01760"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOU16586.1"
                     /translation="MANSNSSKIAGKERGVFQSRRQRTGVFLICGVIVVALALLFPKT
                     HYNITKYKINDIARSSIIAPFDFPILKTEQELEADRLEAIKKVPFVFIQDKDVEKTEL
                     MNLQKFFTEIDRLRLARIKYDESLRNISINRSEESLATLTADSITFFALQKKFLNDYK
                     LNKNSPVLKSLVYQYDPKNANQLSAHVQNQVKSVLSELYVLQILDIPKSQIISEKIAI
                     LQGGEEVLENPNEVLELSEAWTKAKLALQARLNDYTPEVVNAAYDIVVAFLKPNLIYQ
                     KQITEKRQAEAIAKVPISKGVVLKNEKIVDANTRITPEIYQKIESMAKEQARLANVQR
                     SWRRALPVIGDPAIFICQVAVVAIIFTFFMTFLFAYRTDIITSPKKMFLIGIIFILQA
                     VFAAILVNHFKISEYTIPITLAAMSLTILFDSRVAFVGTATICLIIGAQIGGNFNFII
                     VSVFVSTFAIYAVRQLRKRSQIFKSIIYIVLGYCLAIFATEMLKYSPPKSILNNIFYA
                     TINGVFAPFLAYGIIGLLENLFGITTDLSLLELADFNQPLLKLLSHEATGTFTHSVTV
                     GNLAEAAADAIGANALLARVGSYYHDIGKLTKPEYFIENQAFDYNRHDKLAPNLSAII
                     ILNHVKEGLRLANEYKLPQVIQDFISTHHGTTRVEYFYDKALKKSAKPDEINDADFRY
                     PGPKPHTKETGIVMICETIEAVCRSLNKPTMSAIEKVVDSVIESRLKEGQLDECPLTL
                     ADLKKIKGDLKEGTGILPILRGIHHLRVEYPGQEKFSNSSFPSNKKGKNDGSQNL"
     gene            16596..17060
                     /gene="ybeY"
                     /locus_tag="PKX09_01765"
     CDS             16596..17060
                     /gene="ybeY"
                     /locus_tag="PKX09_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOU16587.1"
                     /translation="MTVVKIFNNHPHYTLLKPAIEQVVSYIVNMEQQAVQSITFIASD
                     DKTLNEMKVKLFGIDQLTDTISINYNEIDQPVEGEIYLSLDRIEDNAHTFKVSFEKEL
                     ALVIIHSVLHLLGYNDDNLNDKKQMQALQNFYIQQIKFPRLYRQRRIQPNYE"
     gene            17053..17832
                     /gene="mazG"
                     /locus_tag="PKX09_01770"
     CDS             17053..17832
                     /gene="mazG"
                     /locus_tag="PKX09_01770"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOU16588.1"
                     /translation="MNDELSRYFDELIEIVKTLRGPNGCEWDKAQTRDSLTPYLLEEA
                     HELIDSISENNPSKIKEELGDVLLHIIFQAQIAAEAGEFTIADALQQINAKLIRRHPH
                     VFGETKVENVNEIKKNWEEIKLKEGRQSLMDGLPKSLPALLQARRVQERAAQVGFDWS
                     TIEPIWAKITEETRELQEAIATRKKDEIIAEFGDLLFTMVNLSRFLDIDPEEALRQTV
                     TKFIRRFQAIENELAATGKNFKDTSLEELDKIWNKSKDKES"
     gene            complement(18134..18745)
                     /locus_tag="PKX09_01775"
     CDS             complement(18134..18745)
                     /locus_tag="PKX09_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOU16589.1"
                     /translation="MAEMLLQGLKTIADSAEIMVTVKDSKFVGYCFPVADKSEAVQKI
                     AEISQKHRKATHICWAYRIYENNQLLYYAHDAGEPRGSAGAPILKALTSRQLVKALVV
                     VVRYFGGTKLGIGGLIRAYGATAGKTLDLVEHKPFIQRQTLRIECQLANYSQLAHFLG
                     RFSESSKPEFTENSVIVTVQIEVQKADEFRTELNSIPGLSIVT"
     gene            complement(18757..20433)
                     /locus_tag="PKX09_01780"
     CDS             complement(18757..20433)
                     /locus_tag="PKX09_01780"
                     /inference="COORDINATES: protein motif:HMM:NF039970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU16590.1"
                     /translation="MKIVRQLTFTIVCLIVVEQALAIDQIELIATDESYWQALNAVKN
                     DLRPPRELLKQPVVGYKYSRPSLPLSYQSAGSHFLIHYTLEGYDAVDGTITNTDDVPD
                     FVYETAQAAERSYSLLIDTLGFQSPPVDDIFSPETDIYIINLGGAAYAYTYDENPVQT
                     TDRPYDYTAYTVIDNDFVGYLTTGLNGLRVTVAHEFFHVVQLGYNWWEGNRLANSGGG
                     RTSADDYFLEWCSTWFEERAYPEVNDYLFYVGAFFNSPLKSLWNYDYAYALGFFLRFI
                     LDRYGENLLVKVWEEIKTNYAFESLQAVLADDYNADLADLWNEFYLRCYFSGERYDPD
                     WALSSDAMQFPLLKIPDINKVVFTESAVFNQSIEPFACVPYQVVFDQNWLCGINVNSS
                     TPENFLGRYLFIKNRVRKIAETLSLNQNLLVGNAANNDSLLIFMTNSSKTTTPNLTVT
                     ISKVGDEFRIASTIKNVYPNPYSPGKSGDLIVDLQVGQIVNSIKTNWFDLTGRNVFQK
                     NFDNGLIEIGNYSLSFSSDELRQAQLASGVYIMQIAIGSKNLTRKILLLK"
     gene            complement(20430..21743)
                     /gene="der"
                     /locus_tag="PKX09_01785"
     CDS             complement(20430..21743)
                     /gene="der"
                     /locus_tag="PKX09_01785"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOU16591.1"
                     /translation="MAEPIVAIVGRPNVGKSTLFNRLTQKRKSIVHSEAGITRDRVYE
                     TVNWAGRQFLLIDTGGFIPESTNQITKAIREQVRLAIEEANLVIFVVDASEPMTALDR
                     EIAEMIRRSQRDCLIVANKCDNERLEVNSLEFTELGLGDVFPISALNGRHIGDLLDAI
                     LQRIPRKIAANDDEKEYLQLAIVGMPNVGKSSILNVLAGSEKAIVTDVPGTTRDAIDT
                     KIKYFNEPVTLIDTAGMRKHRQIKDEVEYYSLLRAYRAIERCNVALVVVDTVKGFTRQ
                     DAAIVRQVIDLRKGLVIAINKWDLVQRETNTQVNFKRNLIEQFRELEHYPIIFISAKT
                     HQRVHKILETARMVYENRKMRIGTSELNQFLQDVIDRTPPPANLGKFIKIKYITQVKT
                     EPPVFVFFCNEPRLIKEEYRRFLENQLRRRFGFSGVPLIISFRNK"
     gene            complement(21736..23022)
                     /locus_tag="PKX09_01790"
     CDS             complement(21736..23022)
                     /locus_tag="PKX09_01790"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HOU16592.1"
                     /translation="MVEIVGVKPNSLAEMNGIRRGDQIVSINRQPINDFLDLYFHQSD
                     ENCKVVVLRDGHKKTFQIAKNADEPLGIELSPPRLKACGNNCIFCFIKQNPPGMRPTI
                     YFCDEDYRYSFLLGNYITLTNLSEADLNRIVLQRLSPLYVSIHATDENVRRHIFQLKK
                     PDNLMQKLTFLAQNRIDLHTQIVLIPGVNDGAVLEKTLADLYIFRDSILSVAIVPVGL
                     TAHRNHLPLLPAVDSHLASQLLENIRTWNRRYENRDGEHWVFLSDEFYLLVGQDLPGC
                     KFYGSFYQIENGVGTTRAFLDDFKRQSRKFPHAISKRKRVLFVTGMLAAPVLSRYVTA
                     RLNQINNFSVDILPVINNFFGPSVTVAGLLTGQDILAQAINVDEYDLIVLPPRIINED
                     GVLLDDFYPYQIAETLQKPVQVWDGDFVKLMDEDNG"
     gene            complement(23184..23254)
                     /locus_tag="PKX09_01795"
     tRNA            complement(23184..23254)
                     /locus_tag="PKX09_01795"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23220..23222),aa:Gly,seq:ccc)
     gene            23543..25957
                     /locus_tag="PKX09_01800"
     CDS             23543..25957
                     /locus_tag="PKX09_01800"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOU16593.1"
                     /translation="MSESITIDFKNPKNESPEKNIPENSLSINPENIQVTPEKKPLFS
                     KNAMEVLTKRYLQHDKNGNAVETVEELFRRVAEAVAEAEANYSPRSSIPRTTEKFYQM
                     MIRKDFLPNSPTLMNAGRELGQLSACFVLPIDDSMESIFQTLKDTALIHKSGGGTGFS
                     FSRIRPKKSIVKTTSGVASGPVSFMKVYNAATEAVKQGGTRRGANMGILRVDHPDILE
                     FVDCKKNSNELTNFNISVAVTDEFMEKVRRREQYELVNPVDNTIVGTLNAAEVFKLIA
                     QNAWYNGDPGIIFIDRINQMNPTPKLGDIEATNPCGEQPLLPNESCNLGSINLANFIS
                     ENKIDYKRLGETIDLAVQFLDDVIDINKFPLKKIETMVKKTRKIGLGVMGFADVLFSL
                     KIPYDSDEALQLADELMKFIYNRTKEASTKLGESRGTFPAWNDSIYFPDGPKYRNSTC
                     ITIAPTGTISMIADCSSGIEPIFSLAFTKKVMDGTELLYVNPYFEQACKEAGIYSAEL
                     MHRVIAEGSIQHIAEIPEKIRRVFVTAHDISPEWHVRMQAAFQKWVDAAVSKTCNFPK
                     SATVQDVLDAYWLAYEAGCKGITIYRDGSRDEQVLYVGKQTDQAKSEQAVQPPIIDQT
                     LHPRIRPPETHGVTKRVRTGNGTLYITINEDDYGLCEVFTNIGKAGSQAQVESEAISR
                     LISLALRSGIDARDVVKQLKGIGGPSPIWENGTQILSTPDAIAKALEWYLDEREGKKK
                     TTGNQPSFSLLDVESTTTVKKPEPTSQHTAPHNKKSITTCPECGSTVYHESGCVTCPN
                     CGFSKC"
     gene            complement(26240..27319)
                     /locus_tag="PKX09_01805"
     CDS             complement(26240..27319)
                     /locus_tag="PKX09_01805"
                     /inference="COORDINATES: protein motif:HMM:NF020571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1887 family CARF protein"
                     /protein_id="HOU16594.1"
                     /translation="MALLEVCLVNDLPIIGYLSAILKKPDAIVWLRTPNQKKVVSLIE
                     TALSGQISSTFESREINPNDVPEFIEQCQKVLRDYPEHDVILNISGGSRLHAVLAAEV
                     FKNAGKEVILIDPDNSRIVDVKTGEVKTFQFNLTVNEYIALHGIKMESGTRFDPEIGK
                     RSALSFFIGNNLDRIMPFIDSHRDEWNNMGENKKTVHWKIDDQRLRFVVNYDAEQNVM
                     RFRFGTGENQRTVDIAGDGGNYLFNGGWLRELVFLRVHRQGDDVRLNVRIDRESVPEA
                     MHCESMIDICMMKGCNFYIFQCFSYPITRESFIELQAIHETVRILKATGFIFMSHRPH
                     RGFIERASDIGLEVITGHRIANFSL"
     gene            complement(27484..28602)
                     /locus_tag="PKX09_01810"
     CDS             complement(27484..28602)
                     /locus_tag="PKX09_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOU16595.1"
                     /translation="MEKWRKLVQKSYTTVDQITKALNLPNDELAGLDKVFRIKVNPYY
                     FSLIKKKGDPIYRQVIPDKAELEDCGLEYDPLSEEKDSPVPSIVHRYPDRCLFLVSPE
                     CASYCRFCTRKRKVGDAQKISPQFIQQGIKYIAEHPEIRDVIISGGDPLMLSDQKIEH
                     ILSSLRQIPHIQILRIGTRVPCFLPQRITTKLVRILKKYHPLYINVHFNHPDELTPEA
                     VKALSRLADAGIPLGNQTVLLKGVNDDPEVMKLLMQKLLQARVRPYYIYQADMVYGTE
                     HFRTRVEKGLEIIKALRGWTSGLAVPHFVIDAPGGGGKIPLLPEYVKSITDEEVVMCN
                     YEGREFHYRQAGKPPAPPTCVRDIMAEALVAENDLALN"
     gene            complement(28606..29094)
                     /locus_tag="PKX09_01815"
     CDS             complement(28606..29094)
                     /locus_tag="PKX09_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU16596.1"
                     /translation="MNIIREIKSTDRAEILKILSDTQMFTTAEIRVAMELIDTALTKP
                     NQTDYLIFVAINGQKLAGYVCYGPTPATEGTFDLYWIAVAPQIQGKGIGTDLLTYVET
                     AVQAQHGHLIIIETSSQEKYLPTRNFYLKNGYAIAAQIKDFYRQGDDRVIFVKYFKQE
                     NK"
     gene            complement(29091..30107)
                     /locus_tag="PKX09_01820"
     CDS             complement(29091..30107)
                     /locus_tag="PKX09_01820"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16597.1"
                     /translation="MSSESQKIKILVVFNDLQSVDSNFDPDIISEIAVRDEAAAVYTA
                     LNNCGYVAEMLPILNIDEDLIKINSFQPDVIFNLCEGWQSRTDQEMNVAGLWEMLDLP
                     YTGNSPLTLGLAQNKVLSKNIFKAIGIPTPDFYVYESIPETCSLDFPLIAKPSREDGS
                     IGITQNSVVNDLIELKSIVGELLAKYKQPILVEKYIAGREFNVSILGNENPLVLPPSE
                     INFEQVSEEYFPITSYEAKWLSDHPVYQMTPPVCPAPISAELNEKLTSLSLAVYHALR
                     GRDYGRIDIRMDAAGNLFVLEYNPNPDISPSAGFVRALNAAGISYETFVVFLIKQALN
                     RRRR"
     gene            complement(30107..31159)
                     /locus_tag="PKX09_01825"
     CDS             complement(30107..31159)
                     /locus_tag="PKX09_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOU16598.1"
                     /translation="MKIALTYSTKEGLRKEYRKRFAVDPDDEDIPPDLFAEGDDPQTI
                     EAIVDALQLNNHQVVSFEGDDEVAHGLEQSRPDIVFNVTEGLFGDWRESYVPLICERL
                     GLPYTGSDPLTLAICLNKARCKEILSYYHIPNATFQVIYPGDDGFAENFKFPAIVKPL
                     AEGSSKGIFDNSVVYSKEQADDVIASCFAKYNNEPVIMEKYLAGPEFTVAILGNSPEM
                     EVLPIIGMDFSQLPTGAQPIYSYEAKWIWDRPEKPLNIFTCPAQIDDNLKNQIELLVR
                     RTCRILNIRDWCRLDVRLDENGVAHILEINPLPGILPNPEDNSCFPKAARTAGYTYPA
                     MLNQVVQVAARRYGLS"
     gene            complement(31309..32484)
                     /locus_tag="PKX09_01830"
     CDS             complement(31309..32484)
                     /locus_tag="PKX09_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOU16599.1"
                     /translation="MKNQLSLKIRAVAMTALLIYSLTVTARAQTNLGLWVIRDQLNSK
                     EKIDGFVNFAVLNDIKDLFVQVRGRGDAFYHSEIVVYSELLNNNNWDPLEYTLNQAHS
                     YGLRVHAWVNVFLLWTSDQKPKNENHLLYQHPEWCTVDANGIKDIQRRATDFNTLGTE
                     GIYLSPLLPEVRKYLIDIIQELATNYDLDGIHLDYVRYAKNCYDYNPIGRILFREKYG
                     IDPLLLTLSDKSLYKGLELAEIDSLMLKWEDFRRDAISDFVHNARKNLREIRPSLQLS
                     AAVKPDPDDACKNFFQDWQYWLRQDWLDFVVLMNYTKDTKQFQKILRKIDPNINKNNV
                     WVGISVYNQSQYEAMTKTILTLNNQFQNIVFFSYETFLKDPQYYPVVRKAFEIAHPR"
     gene            complement(32505..33098)
                     /gene="rplQ"
                     /locus_tag="PKX09_01835"
     CDS             complement(32505..33098)
                     /gene="rplQ"
                     /locus_tag="PKX09_01835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOU16600.1"
                     /translation="MRHQKKVAKLGRSTDQRKALIQSLTKAMLINGRIKTTDAKAKEL
                     RRVVERLITYGKRDTVHSRRLAFRFIQDRDLVNKLFEDIAPQYANRTGGYTRIIKLGY
                     RDNDNAAVSLIEFVDYKQPEEKKKKAGSEATKSVQGKKEEKSTEVEKEQTPAKTKPTK
                     KTQAKKEEKQSAQAEVKEDIPPENISGEKAEPASETE"
     gene            complement(33120..34100)
                     /locus_tag="PKX09_01840"
     CDS             complement(33120..34100)
                     /locus_tag="PKX09_01840"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOU16601.1"
                     /translation="MTVYDQIIARVVKDENSNDTHGIYYLQPLERGFGITIGNALRRV
                     LLTSIPGAAICSIKIDGVLHEFSTIPGVREDVAEIILNLKQVRLKLFDPKPDKVTLHF
                     KGAGEFTAGMIGENITQFKILNPDLHICTMNEDADFNIEIRIARGTGWVPAEKNKLPD
                     YPIGTIFIDSIFSPILNVSYKVENVPGTAREILEKLTLEITTDDSITPDESLDLASKI
                     LRDFFSLFSRVEAKPIEFQREEPSEDVLKIRNLLKKSVDEMELSVRSYNCLKANNIRT
                     IADLVSHEEADMLKFKNFGRKSLNELMTKLKSMGLEFGMDVDKYLVEKEE"
     gene            complement(34112..34744)
                     /gene="rpsD"
                     /locus_tag="PKX09_01845"
     CDS             complement(34112..34744)
                     /gene="rpsD"
                     /locus_tag="PKX09_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOU16602.1"
                     /translation="MARYTGPVCKLCRREGKKLFLKGERCNSTKCSFETKSYIPGQHG
                     SQTAHPRLSNYGIQLREKQKIKRIYGVLEKQFRQYFELANRQKGVTGTNLMKILERRL
                     DNVVYRLGFASSRRMARELVSHRHFTVNNRVVDVPSYQLKAGDVIQVREKSKKLQVIH
                     DSMKRIRGEHELPWLILDKAKMQGIFIQVPERDQMNVDVNEQLVVELYSK"
     gene            complement(34767..35159)
                     /gene="rpsK"
                     /locus_tag="PKX09_01850"
     CDS             complement(34767..35159)
                     /gene="rpsK"
                     /locus_tag="PKX09_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOU16603.1"
                     /translation="MPPKRSTVKKKKRTKVESEGIATIKATFNNTIISLTDNYGNVIS
                     WASAGTAGFKGSRKNTPYASQQAATKAAQEAINLGLQRVEVRVKGPGAGREAAIRALN
                     LAGLQISAIKDVTPIPHNGCRPPKRRRV"
     gene            complement(35177..35563)
                     /gene="rpsM"
                     /locus_tag="PKX09_01855"
     CDS             complement(35177..35563)
                     /gene="rpsM"
                     /locus_tag="PKX09_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOU16604.1"
                     /translation="MARIAGVDLPNEKKVKIALAYIYGIGRANAMDILQKAGVDPEIR
                     VKALTEEQVRDIRNVISNEYKVEGTLRTEVQMNIKRLMDIGCYRGLRHRRGLPVRGQR
                     TKTNARTRKGPRRTVSRKKVKVGKKG"
     gene            complement(35583..35696)
                     /gene="rpmJ"
                     /locus_tag="PKX09_01860"
     CDS             complement(35583..35696)
                     /gene="rpmJ"
                     /locus_tag="PKX09_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002344960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOU16605.1"
                     /translation="MKVRSSVKKICNKCKIIRRKGVVRVICTNPKHKQRQG"
     gene            complement(35713..35931)
                     /gene="infA"
                     /locus_tag="PKX09_01865"
     CDS             complement(35713..35931)
                     /gene="infA"
                     /locus_tag="PKX09_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOU16606.1"
                     /translation="MVKEKLIKVDGIIKENLPNASFRVVLENGHEVLAHVSGKMRMHF
                     IKILPGDKVTMELSPYDLTKGRITYRYK"
     gene            complement(35943..36692)
                     /gene="map"
                     /locus_tag="PKX09_01870"
     CDS             complement(35943..36692)
                     /gene="map"
                     /locus_tag="PKX09_01870"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOU16607.1"
                     /translation="MVHIRTAEEIEKIRESCRIVSETLDLLSREIKPGITTLYLDHLA
                     AEFIKSQGGHSAFKGYNGFPCNICTSIDEQVVHGIPSNRVLKEGEIISIDVGVKKNGY
                     YGDAAMTFAVGTVSDDRLQLMRVTEDSLYQGIAQARPGNHLSDIGHAIQTHVESYGYG
                     VVRELVGHGVGRELHEPPEIPNYGEPGRGPVLKPGMCLAIEPMITMGSYEVRLLKDGW
                     TVITEDHSPSAHYEHTIAIGPDGPQILSQRG"
     gene            complement(36702..38042)
                     /gene="secY"
                     /locus_tag="PKX09_01875"
     CDS             complement(36702..38042)
                     /gene="secY"
                     /locus_tag="PKX09_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOU16608.1"
                     /translation="MIQQFQSIFKIPELRKRIGYTLALLIVFRIGAHIPIPGIDTEVL
                     GAAMSSFANTLFGLYDMFSGGAFAQATLFALGIMPYITASIILQLLGAVIPYFQKLQK
                     EGEEGRKKLTQMSRYGTVIIAALQSFGVAQFLMSPQMSARVGNQMLPVVPRPGVGFVL
                     MTMLTITTGTVLLMWLGERMTERGIGNGTSMIIMIGILARLPNVFVSEITLIREGVRG
                     LFTEIILIVLMIVIVGFVVLLTQGTRRIPVQYAKRVVGRKVYGGQATHIPLRVNTAGV
                     MPIIFAQSIVFIPSTIATFFPNSDFVQGMMRVFSVQSIVYWLIYGVLIVFFTYFYTAI
                     AFNPVEVADNLKKYGGFIPGIRPGKKTAEFIDNILTKVTLPGSIFLAFIAVFPYILMK
                     IMDINYDLASFFGGTSLLIVVGVALDTLQQIESHLLMRHYDGFLKSGKLKGRRG"
     gene            complement(38039..38476)
                     /gene="rplO"
                     /locus_tag="PKX09_01880"
     CDS             complement(38039..38476)
                     /gene="rplO"
                     /locus_tag="PKX09_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOU16609.1"
                     /translation="MGLNKLKPAYGSLKNKKRLGRGPGSGHGGTAGRGNKGTGARGSN
                     KKPVYVEGGQMPIYRRLPKRGFKNLFKEEFQVVNLDHIARLQITEVNPEILQQKGLIK
                     SLKKPVKILGRGELSGAITVVAHAFSTRAKEKITASGGKVELL"
     gene            complement(38480..38683)
                     /gene="rpmD"
                     /locus_tag="PKX09_01885"
     CDS             complement(38480..38683)
                     /gene="rpmD"
                     /locus_tag="PKX09_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOU16610.1"
                     /translation="MTKKDKTAMLKITQVKSGIGYCEKTKRTLLALGITKMNQTVLLP
                     DNPAIRGMVNSIPHLLRVEEIKE"
     gene            complement(38680..39189)
                     /gene="rpsE"
                     /locus_tag="PKX09_01890"
     CDS             complement(38680..39189)
                     /gene="rpsE"
                     /locus_tag="PKX09_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOU16611.1"
                     /translation="MRKTIDSTQLELLEEKVIKINRVAKVVTGGRRFRFNALVAIGDG
                     KGHVGIGLGKALEVSSAVAKGKENAKKNLVKIPVINGTIPHEVQAKYGAAKVILRPAT
                     PGTGVIAGGAIRAIMESLGVRDVLSKSLGSNNSHNVVKATMLALSKLKDPYMVAHRRE
                     KTLQEIFGS"
     gene            complement(39208..39582)
                     /gene="rplR"
                     /locus_tag="PKX09_01895"
     CDS             complement(39208..39582)
                     /gene="rplR"
                     /locus_tag="PKX09_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOU16612.1"
                     /translation="MNKVKNIKAFARQRRQKRNRHKIQGTSERPRLTVFRSLNHIYAQ
                     VVDDNLHKSLLAVSDLTPEVREQIKSNTKKSDRSALVGKTLAEKAVAKGITKVVFDRS
                     GYQYHGRVKALAEAARQAGLEF"
     gene            complement(39594..40136)
                     /gene="rplF"
                     /locus_tag="PKX09_01900"
     CDS             complement(39594..40136)
                     /gene="rplF"
                     /locus_tag="PKX09_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOU16613.1"
                     /translation="MSRIGKKPIEIPKEVNVTIQDCSITVKGPKGELKWDFPSDMKVQ
                     INDSQIIVTRPSDSRNHKALHGLTRVMIANMIQGVLQGFSKTLEIEGVGYSAEMKGKS
                     VQINVGYSHAILVTPPEGVSFEVPKALTIVVKGIDKQKVGQIAAKIRSIKPCEPYKGK
                     GIRYQNEHIRRKAGKKVGVK"
     gene            complement(40160..40558)
                     /gene="rpsH"
                     /locus_tag="PKX09_01905"
     CDS             complement(40160..40558)
                     /gene="rpsH"
                     /locus_tag="PKX09_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOU16614.1"
                     /translation="MAVTDPIADYLTRIRNAQAANHRWVDIPASNLKKRISLILKHEN
                     FIKDFILIKDGKQGLIRIYLRYSKTGEPMIEGLQRISKPGRRVYAASDNIPRVVGGMG
                     IAILSTPMGVLTDKVARKNNVGGEVLCYVW"
     gene            complement(40575..40760)
                     /locus_tag="PKX09_01910"
     CDS             complement(40575..40760)
                     /locus_tag="PKX09_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOU16615.1"
                     /translation="MAKKSLIAKARRNPKFSTRRVNRCSICGRPRGYYRKFGLCRLCF
                     RELALRGEIPGITKASW"
     gene            complement(40762..41346)
                     /gene="rplE"
                     /locus_tag="PKX09_01915"
     CDS             complement(40762..41346)
                     /gene="rplE"
                     /locus_tag="PKX09_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOU16616.1"
                     /translation="MTAYETEIVPAMVKKFGYKNIMQVPKLVKISINMGVGKTKEDPA
                     SLKKAVEDIRIITGQQPMVTKAKRAISNFKIKTGDPVGCAVTLRRFRMYEFMDRLIST
                     ALPRVRDFSGLSDKSFDGRGNYSIGLKEQIIFTEIDYDKVDHIRGMNITFTTTAKTDT
                     EAYELLSLLGLPFKKRAIKESTTGGEVKTAMEIN"
     gene            complement(41393..41707)
                     /gene="rplX"
                     /locus_tag="PKX09_01920"
     CDS             complement(41393..41707)
                     /gene="rplX"
                     /locus_tag="PKX09_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOU16617.1"
                     /translation="MLHIRKNDTVMVITGESKGKIGKVLKVFPDKERVIVEGVNFIKR
                     HTRPNQQNPQGGIIEKEAPIHISNVKLVHAGQATKIGYRTLKDGKKVRVARETGEIID
                     AI"
     gene            complement(41720..42088)
                     /gene="rplN"
                     /locus_tag="PKX09_01925"
     CDS             complement(41720..42088)
                     /gene="rplN"
                     /locus_tag="PKX09_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOU16618.1"
                     /translation="MIQQESRLKVADNTGAKEVMVIRVMGGSHRKYGTVGDTIVVTIK
                     QATPGGMVKKGEKSKAVVVRTTKEVRRKDGSYIRFDDNAAVLIDNNLEPRGTRIFGPV
                     ARELREKNYMKIVSMAPEVL"
     gene            complement(42112..42363)
                     /gene="rpsQ"
                     /locus_tag="PKX09_01930"
     CDS             complement(42112..42363)
                     /gene="rpsQ"
                     /locus_tag="PKX09_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOU16619.1"
                     /translation="MISSDRRKILIGTVISHKQDKTAVVSVERTVKHSTYGKYIRRTS
                     KFYVHDAENKCHEGDKVKIVESRPLSRLKRWRLLEIIER"
     gene            complement(42382..42600)
                     /gene="rpmC"
                     /locus_tag="PKX09_01935"
     CDS             complement(42382..42600)
                     /gene="rpmC"
                     /locus_tag="PKX09_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOU16620.1"
                     /translation="MKKSTLDELTVNELETKLRDNLTALENLRFQKALQQLENPLKIR
                     HVKKEIAQIRTVLHEYELGIRSKDKPNK"
     gene            complement(42602..43021)
                     /gene="rplP"
                     /locus_tag="PKX09_01940"
     CDS             complement(42602..43021)
                     /gene="rplP"
                     /locus_tag="PKX09_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOU16621.1"
                     /translation="MLAPKKIKWRKQQRGRMTGIATRGNAVSFGHYGLKAMEPGWIDS
                     RQIEAVRVAISRQIRKHGKMWIRIFPDKPITKKPAETRMGKGKGTPEFWVAVVKPGRI
                     LFEIEVASEQEAKDIFKTATHKLPIKTKVVARRELGA"
     gene            complement(43045..43671)
                     /gene="rpsC"
                     /locus_tag="PKX09_01945"
                     /pseudo
     CDS             complement(43045..43671)
                     /gene="rpsC"
                     /locus_tag="PKX09_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654291.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            complement(43683..44051)
                     /gene="rplV"
                     /locus_tag="PKX09_01950"
     CDS             complement(43683..44051)
                     /gene="rplV"
                     /locus_tag="PKX09_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOU16622.1"
                     /translation="MEAKAVHRYVRQSARKVRLVADLVRGKDVETALNLLHFNSKKAS
                     ENVEKVVRSAVSNLLNSEGGSKLNPEDLYLKTIYVDGGSIAKRFRAGSLGRASIIRRR
                     LCHITVVVAEKENKQKSKVQ"
     gene            complement(44060..44335)
                     /gene="rpsS"
                     /locus_tag="PKX09_01955"
     CDS             complement(44060..44335)
                     /gene="rpsS"
                     /locus_tag="PKX09_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOU16623.1"
                     /translation="MSRSLKKGPYIDVKLMKKIEQMNSSGKKRVIKTWARRTTISPEF
                     VGHTFAVHNGNKFIPVFVTENMVGHKLGEFAHTRIFRGHSGAEKKGA"
     gene            complement(44352..45179)
                     /gene="rplB"
                     /locus_tag="PKX09_01960"
     CDS             complement(44352..45179)
                     /gene="rplB"
                     /locus_tag="PKX09_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOU16624.1"
                     /translation="MGIKTIKPITPGQRFKTVDTFEDITKKRPEKSLLEPITQSAGRN
                     NYGRVTSRHRGGGQKRAYRNIDFKRNKYGIEATVKAIEYDPNRSARIALLFYKDGEKR
                     YIIAPDGLVTGSVVLSGEEAPIKVGNCLPLKNIPAGTFVHNIEMRPKKGGQIARGAGT
                     AAQIMAKEGKYVTLKMPSGEMRLINEECYATIGVVSNKDHENISIGKAGRSRWMGRRP
                     HVRGVAMNPIDHPMGGGEGKSSGGRHPCSPWGQLSKGYRTRKKRNPSNKFIIKRRTK"
     gene            complement(45184..45504)
                     /gene="rplW"
                     /locus_tag="PKX09_01965"
     CDS             complement(45184..45504)
                     /gene="rplW"
                     /locus_tag="PKX09_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009353943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOU16625.1"
                     /translation="MSSKSIIIKPILTEKINELQESQNKYGFKVHPSANKIEIKKAVE
                     DKFGVKVEKVATQNIMGKTKLMTVRSGGRVIRTTGRRSNWKKAIVTLKEGEKIDFYQG
                     ETAV"
     gene            complement(45507..46154)
                     /gene="rplD"
                     /locus_tag="PKX09_01970"
     CDS             complement(45507..46154)
                     /gene="rplD"
                     /locus_tag="PKX09_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOU16626.1"
                     /translation="MSIEFSVYKLDGSDSGQKLVIETPAFDIEPHDHAIYLAVKTEMT
                     NSRQGTHSTKTRGEVRGGGKKPWKQKGTGRARSGSRRSSVWVGGGRAFGPKPHPYKMK
                     INKKVKSLARQSALAYRYREGALKIIEELAFDEPKSRNIRELLKKFDLLDKKVTILTA
                     NLNTNLLLASRNFYNVLILEAEKASTYDLLDCEVLLIDKSGFEKLNNALLGDSRN"
     gene            complement(46160..46780)
                     /gene="rplC"
                     /locus_tag="PKX09_01975"
     CDS             complement(46160..46780)
                     /gene="rplC"
                     /locus_tag="PKX09_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOU16627.1"
                     /translation="MMIGIIGKKIGMSQIFKENGELIPVTVIAAGPCKVVQVKTNEKD
                     GYASVQLGFMEAKERLMAKPNLGVFKKNNITPLKYLVEIRDYDPKFCQVGETVTVALF
                     NPGDKVKITGISKGKGFQGVVKRHNFGGGPKTHGQSDRLRAPGSIGASAYPSHVIKGV
                     RMAGRLGGKRCTVNNLTVVKIDPESNLLYIKGAVPGSKNSILTIRR"
     gene            complement(46789..47097)
                     /gene="rpsJ"
                     /locus_tag="PKX09_01980"
     CDS             complement(46789..47097)
                     /gene="rpsJ"
                     /locus_tag="PKX09_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOU16628.1"
                     /translation="MPGQRIRISLKAYDHNLLDKSTRKIVATAKSTGAMVSGPIPLPT
                     QRTVYTVLRSPHVDKKSREQFQTKIHKRLIDILNSTSKTVDALMKLDLPAGVDIEIKV
                     "
     gene            complement(47109..48299)
                     /gene="tuf"
                     /locus_tag="PKX09_01985"
     CDS             complement(47109..48299)
                     /gene="tuf"
                     /locus_tag="PKX09_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOU16629.1"
                     /translation="MAKQQFKRTKPHLNIGTIGHVDHGKTTLTAAITMVLHRQGLADE
                     RTFDSIDNAPEERERGITINTSHVEYETKKRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAFDGPMPQTREHVLLAYQVNVPSIVVFMNKVDLVDDPELLDLVEMELRDLLNK
                     YHFPGDEVPIIRGSALEALNHPDDPEKTKCIFELMEAVDNYIPDPVRAIDKPFLMPVE
                     DVFSITGRGTVGTGRIDRGKVRVGDEVEIVGLGPTRKTVVTGVEMFRKILDEGEAGDN
                     VGLLLRGIEKDWLQRGMVIAAPKSITPHRKFKGQVYVLKKEEGGRHTPFFNGYRPQFY
                     FRTTDVTGVVTLPEGVDMVMPGDNVVMSVELISEIAMEKELRFAIREGGRTVGAGVVT
                     EIIE"
     gene            complement(48311..50407)
                     /gene="fusA"
                     /locus_tag="PKX09_01990"
     CDS             complement(48311..50407)
                     /gene="fusA"
                     /locus_tag="PKX09_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOU16630.1"
                     /translation="MKSENLSKVRNIGIMAHIDAGKTTTTERILFYTGKVHKIGEVDE
                     GSATMDWMEQERERGITITSAATTCYWNQHRINIIDTPGHVDFTAEVERSLRVLDGAV
                     AIFCAVGGVEPQSETVWRQADKYHIPRIAFVNKMDRTGADFFNVLDMIEKRLKAQPVA
                     VTLPLGSGELFTGIIDLITQQAMLYDDNSLGSQYYYIEIPKDLKNIATQYRQKLIEEV
                     ANFDDVVFAKYLNNEDITPQELQNSLRKGTLENKIVPVLCGSAFRNKGIQPLLDAIVN
                     YLPSPFDMPPIEGVNLKGTPIKRYPRPDEPLSALIFKIMSDPHVGRLSFIRIYSGVIS
                     KGDMVFNSTIQKKERISRLLLMHANKREDCEMLEAGEIGAIVGLKYSYTGHTLCDPKN
                     PIILESMQFPEPVIKISIEPKSKADEENLINALNKLADEDPTFEISTDEETGQILIAG
                     MGELHLEILVERLIREFNVKAHIGKPQVSYRETVVSVAEGEGRFVRQTGNKSQFAVVR
                     LRIEPDSASNEVRIENRIVPGTLPKEFVPAIEKGIRNHVKSGVLAGYPLQKVRVIITG
                     AEYREEESTELAFEVAAAMAFENALHKAEPALLEPIMALEVVVTDEYLGDVLSDLNTR
                     RANIINVNKRGDANVIDAEAPLREMFGYATDLRSISQGRAVFTMKFSQFDYCDKRVQR
                     EIIEKARGFVPEFLKN"
     gene            complement(50428..50895)
                     /gene="rpsG"
                     /locus_tag="PKX09_01995"
     CDS             complement(50428..50895)
                     /gene="rpsG"
                     /locus_tag="PKX09_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOU16631.1"
                     /translation="MSRRKKVISREVLPDPKYGDIMVARFINYLLRKGKKSVAEASFY
                     DAIAQIEAKHKGNGLDVFKKAINNVSPIMEVKSRRIGGATYQVPIEVRPERRFALASR
                     WLITYAKARPGKSMAEKLAAEILAAANGEGNAIKKREDTHKMAEANKAFAHFR"
     gene            complement(50911..51282)
                     /gene="rpsL"
                     /locus_tag="PKX09_02000"
     CDS             complement(50911..51282)
                     /gene="rpsL"
                     /locus_tag="PKX09_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOU16632.1"
                     /translation="MPTINQLIRKGREVVSKKEKAPALRGSPQKRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGIEVTAYIPGEGHNLQEHSMVLIEGGRVKDLPGVRYHIIRGTL
                     DSSGVNDRKNSRSRYGTKRPS"
     gene            complement(51358..55632)
                     /gene="rpoC"
                     /locus_tag="PKX09_02005"
     CDS             complement(51358..55632)
                     /gene="rpoC"
                     /locus_tag="PKX09_02005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOU16633.1"
                     /translation="MNYTKPSTFRSEFESITIHLSSPETILTRSHGEVLKPETINYRS
                     YKPEKDGLFCEKIFGPVKDWECHCGKYKRIRYKGIICDRCGVEVARKKVRRERMGHIT
                     LAVPVVHIWFLKSIPSKLYYLLDIPTKNLEEIVYYERYVVLNQGKSDLKYKTVITEEE
                     YFENMQAYGPKTELFDEEEAKNYFIAKTGGEAIRELLQKVDIEGLVHSLKEELKIKKS
                     PTQKAEILKRLKVVMAFLNQEGKPINKPEWMVVTVLPVIPPDLRPLVPLEGGRFAASD
                     LNDLYRRVIIRNNRLKQLIEIKAPDVILRNEKRMLQEAVDSLFDNSRRRTEVRSGTRR
                     PLKSLSDMLRGKEGRFRQNLLGKRVDYSGRSVIVVGPDLRLNECGLPKDMAIELFKPH
                     IIHALLRRRLAKTPKAAKTMVERKDAEVYRVLEYVVKDYPVLLNRAPTLHRLGIQAFQ
                     PKLIEGKAIQIHPLVCAAFNADFDGDQMAVHIPLTFEAQVECRLLALSSHNILHPAHG
                     HPIAIPSQDMVLGVYYITRQKEGARGAGLVFSSPEEAIYAYEIGEVDLHAKVKIRIDG
                     KIIETTIGRALFNSLLPPGMGYYNELISKRSLEKIVGIVFQEAGNYETVKFLDNLKNI
                     GFKYATRSGVSISISDVLIPEEKEKILQESFERVEDIQRKFRKGILTEGERYNKVIDN
                     WTHTTNRLSEVMLEHMATDRQGFNPVYMMADSGARGSGEQIKQLAGMRGLMAKPQKSM
                     TGQVGEIIETPITSNFKEGLTVHEYFISTHGARKGLADTALKTADAGYLTRRLVDVAH
                     DVVISEEDCQTINGIEVTALKKEENIIESLADRIIGRVAQEDIYNPESEDEILVEVGE
                     EITEKTALKIQSAGIERVRIRSVLTCESLRGVCAKCYGRNLANGKLIKVGEAVGIMAA
                     QSIGEPGTQLTLRTFHYGGTAARIVEESETVAKTKGYVQFSSNYVAEKYKDSDYSVVL
                     SHNSVLNLVDDNNRITSTYNIPYGAHVFVNEGQLVKAGHTLFRWDVYMDQILALYSGK
                     VRFVDLIENVTYNIEYDESGHQTMTVIESKDRSLQPRIDIVAQENGKEVVYPGYILPV
                     RAMLVVRDGDEVVKGDILAKKPKEIGKTSDITGGLPRVAELFEARRPKDPAVISEIDG
                     KVTFGKTEKGVREIIVTGIDGTTKKYKIPYGRYVLVNQGDEVRAGERLCEGPVAPQDI
                     LAVQDPRKVQEYLVNEIQEVYRLQGVRINDKHIEVIVRQMMQKVRIEDPGDTNLLEKD
                     RVNRHELIEENNRIKDYVVIVNAGDSDWDEGDVVSKKEFAKFNRLLKEEGKNPAKARP
                     ARPAQFTEMLLGITRASLNTESFISAASFQETTRVLTDAAVAGKTDYLRGLKENVIMG
                     RLIPAGTGLRKYKDISVIEPEEEIEEWKKLEEQKKEKENELAALQK"
     gene            complement(55660..59442)
                     /gene="rpoB"
                     /locus_tag="PKX09_02010"
     CDS             complement(55660..59442)
                     /gene="rpoB"
                     /locus_tag="PKX09_02010"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOU16634.1"
                     /translation="MSNTLPLQKERKSFAKVKIVQKMPNLLEVQLKSFDEFLQKDTMP
                     LERKNIGLESAFRNVFPVEDTHGNYLLEYKYYTIGEPRYTIKECLERGVSYTVPLKVR
                     LVLHISEEGISKGEYQAGIEQDIFFGNVPFMTEKGTFIINGAERAIVSQLQRSPGVFF
                     DETEHTNGMKIYSARVIPFRGSWIDFVIDTRDILSAIIDRRRKFPATILLRAFGYPTN
                     EDIVNLFGIGKRFSLDGDSKELLGKMLAENVIDPETGEVISEIKEEIVVDKDFLKKCK
                     QHGITSLLVFTTDDEIERNIFLNTFRKDTQTNDEESALYFLYRSLRTGEPPNLDIAKK
                     FVERLFFSPKKYDLGQVGRYRINKKFNLKVPLEECVLTRDDIVEIMKRIVDMKKGNLG
                     PDDIDHLGNRRVRNVGEQLTNQFNLAFTRMARTIRERMNLRESENLTPQDLINSRIIT
                     SVINSFFGTSQLSQFMDQTNPLAEITHKRRVSSLGPGGLSRERAGFEVRDVHYTHYGR
                     LCPIETPEGPNIGLIASLATYAYVNDLGFIETPYRRVEKLNGIATVTDKVEYLSADDE
                     DREIIAQANEPLDEQGHFINSRVRARVRGEFPIVTPDEVKYMDVSPEQIVSVSAALIP
                     FLEHDDANRALMGSNMQRQSVPLLKPERPIVGTGLEGVVARDSRSVVLAPVNGIVEEV
                     TADHIVIRTDTENELYLDENQGKVRIELAKFLRTNQDTCINQKPLIKEGDRVKAGDAI
                     ADGCATNDGELALGRNVLVAFMPWRGYNFEDAIILNERLVYDDVFTSIHIKEFELEVR
                     DTKRGNEELTREIPNVSEEATKNLDANGIIRVGAEVEPNDILIGKVTPKGETDPTPEE
                     KLLRAIFGEKASDVKDASKRADPGIRGVAVDTKLFTRKSKVSRMEEKRQIDEETKRSV
                     QKHAYLKGRRDLLLKNILKGATSATIKNLTNGKVAIKSGIQFNDEILDQLDFDLLSLE
                     TPWTNDPEINEKVEKIVKSYFILSQRIDEELEQTIYKIKVGDDLQPGVLQLAKVYIAS
                     KRKVQVGDKMAGRHGNKGVVSIIVPKEDMPFLEDGTPVDIILNPMGVPSRMNLGQLYE
                     TMLGWAGRELGVYYETPVFNGASEQEVQEELRKAGLPEIGKVNLIDGQIGEQFDNPVT
                     VGYIYMMKLSHLVEDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAA
                     YTLQEILTVKSDDVDGRSKVYNAIVKGENFSDFGVPESFNVLLKELQGLCIEMDIN"
     gene            complement(59548..59940)
                     /gene="rplL"
                     /locus_tag="PKX09_02015"
     CDS             complement(59548..59940)
                     /gene="rplL"
                     /locus_tag="PKX09_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOU16635.1"
                     /translation="MANITRADVLEYLEKANMLEISELIKDIEKKFDVKAAAPVAVAA
                     TVAQPGATAEAEAVEEQTEFNVILKEVGPNKINVIKVVRAATNLGLKEAKDLVDGAPS
                     TVKEGISKEEAAELKKQLEEAGAKVEIK"
     gene            complement(59970..60497)
                     /gene="rplJ"
                     /locus_tag="PKX09_02020"
     CDS             complement(59970..60497)
                     /gene="rplJ"
                     /locus_tag="PKX09_02020"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOU16636.1"
                     /translation="MPNQKNIQSLETISEKFQKARGIYFTNYKGLNVKEITDLRKELY
                     AADVEYKVVKKTLTKMAAKNSGLDSIEPLLDGQTALAISYTDPAIPGKVINTFIKKNK
                     LDKLVITGCIYDGVLFGADQVEKVINLPSRRELLASLLATLNAPMSQLTMVLRATLNQ
                     LVGTLNSLNEQKKQS"
     gene            complement(60512..61201)
                     /gene="rplA"
                     /locus_tag="PKX09_02025"
     CDS             complement(60512..61201)
                     /gene="rplA"
                     /locus_tag="PKX09_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOU16637.1"
                     /translation="MNISKRVEQNSKKVDRKKEYPLREALELLKNCNGAKFDETVEAH
                     VNLGVDPRHADQNIRGTVTYPHGTGRKVRVLVLTKSKVDEALQAGADYAGLEEYLEKI
                     QNGWTDVDVIIATPDVMGQVGKLGKILGPKGLMPNPKSGTVTNDVAAAIKEIKAGRVE
                     IRVDKTGIVHTIVGKKSFPIEHLEENVQTLISTLMRLRPPSVKGAYIQKVTLTTTMGP
                     GVRVDKSSIIA"
     gene            complement(61216..61641)
                     /gene="rplK"
                     /locus_tag="PKX09_02030"
     CDS             complement(61216..61641)
                     /gene="rplK"
                     /locus_tag="PKX09_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOU16638.1"
                     /translation="MAKKVLATIKLQIEAGKANPSPPVGPALGQHSVNIVEFCKAFNA
                     RTQDQAGYIIPVVITVYSDRSFTFITKTPPASVLLLKAAKVEKGSGEPNKSKIGSVTM
                     DQVMEIAKIKMPDLNAHTVEKAAEMIKGTARSMGISINE"
     gene            complement(61649..62176)
                     /gene="nusG"
                     /locus_tag="PKX09_02035"
     CDS             complement(61649..62176)
                     /gene="nusG"
                     /locus_tag="PKX09_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOU16639.1"
                     /translation="MEWYVLRTYSGKEKKVREAILIEAEHAGMKDIIKDVLVPSENIM
                     EMREGKKRVRNKVFFPGYILVNMDITKESQYLIENIPGVMNFVGAAGKPQPLKEDEVK
                     RVMGEVEKKDGREVLAIPFKMGDPVKVIDGPFIDFRGVVQEVNEDKRKLKVSVSIFGR
                     PTPVELDFLQVELEK"
     gene            complement(62187..62369)
                     /gene="secE"
                     /locus_tag="PKX09_02040"
     CDS             complement(62187..62369)
                     /gene="secE"
                     /locus_tag="PKX09_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOU16640.1"
                     /translation="MIKRIREFFEGVVFEMKKVSWPTWSELKSSTAVVLIFSLILSVF
                     LFVVDLILSKIMNVIF"
     gene            complement(62418..62490)
                     /locus_tag="PKX09_02045"
     tRNA            complement(62418..62490)
                     /locus_tag="PKX09_02045"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(62455..62457),aa:Trp,seq:cca)
     gene            complement(62502..62651)
                     /gene="rpmG"
                     /locus_tag="PKX09_02050"
     CDS             complement(62502..62651)
                     /gene="rpmG"
                     /locus_tag="PKX09_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002835319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOU16641.1"
                     /translation="MRELITLECTVCKRRNYSSDKNKKLQPNRVEYKKYCRWCKKHTV
                     HKETR"
     gene            complement(62667..63857)
                     /gene="tuf"
                     /locus_tag="PKX09_02055"
     CDS             complement(62667..63857)
                     /gene="tuf"
                     /locus_tag="PKX09_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOU16642.1"
                     /translation="MAKQQFKRTKPHLNIGTIGHVDHGKTTLTAAITMVLHRQGLADE
                     RTFDSIDNAPEERERGITINTSHVEYETKKRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAFDGPMPQTREHVLLAYQVNVPSIVVFMNKVDLVDDPELLDLVEMELRDLLNK
                     YHFPGDEVPIIRGSALEALNHPDDPEKTKCIFELMEAVDNYIPDPVRAIDKPFLMPVE
                     DVFSITGRGTVGTGRIDRGKVRVGDEVEIVGLGPTRKTVVTGVEMFRKILDEGEAGDN
                     VGLLLRGIEKDWLQRGMVIAAPKSITPHRKFKGQVYVLKKEEGGRHTPFFNGYRPQFY
                     FRTTDVTGVVTLPEGVDMVMPGDNVVMSVELISEIAMEKELRFAIREGGRTVGAGVVT
                     EIIE"
     gene            complement(63938..64010)
                     /locus_tag="PKX09_02060"
     tRNA            complement(63938..64010)
                     /locus_tag="PKX09_02060"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63975..63977),aa:Thr,seq:ggt)
     gene            complement(64054..64124)
                     /locus_tag="PKX09_02065"
     tRNA            complement(64054..64124)
                     /locus_tag="PKX09_02065"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(64090..64092),aa:Gly,seq:tcc)
     gene            complement(64133..64216)
                     /locus_tag="PKX09_02070"
     tRNA            complement(64133..64216)
                     /locus_tag="PKX09_02070"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(64180..64182),aa:Tyr,seq:gta)
     gene            complement(64377..64449)
                     /locus_tag="PKX09_02075"
     tRNA            complement(64377..64449)
                     /locus_tag="PKX09_02075"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(64414..64416),aa:Thr,seq:tgt)
     gene            complement(64946..66445)
                     /locus_tag="PKX09_02080"
     CDS             complement(64946..66445)
                     /locus_tag="PKX09_02080"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU16643.1"
                     /translation="MKIIKVCLLTLLMLASSLLFGQAYQVTAMNGYSVNKPKLDVVGD
                     QAMITFATNFNFYSFPLTGPTSPIENPVKPDMNAWGPTNVDIAHANGQVYIVYTDFNQ
                     NSEYGQYDVRYVSQTIGSPEWTIPIILDTVSIGHSIMMTYTNQDLQVGANGAYLFWRN
                     QIDDNSIYFSKSYGSLFSQKRKVLGDSLSLIKDYSFTVIPVEGTDHLFLIYAQDSSLF
                     FISSSDEGSTFSEPTKIDSLEGFWSDFYDLQIVGLLNGTIYAKGQFWQFVQHWDETEQ
                     PGEDEPPMDMDGTRLYRSTDFGAHWSIVNTLPASNSQIALTPGGTIVLTQYPSDPNLY
                     VKTSTDAISWSDSVRINPVENTAAGWSGIGFDVCIIDETTMAVAWIDTTTGHDEIWYR
                     TLTIPTPPLVSVEKSGINTPQEAILVQNYPNPFNPVTKFSYQLPSESFVELSIFDING
                     RLVDKIVNQFQPAGYYSVQWNAGNYSTGVYLYKFRADNFQQVGKCLLLK"
     gene            66975..>67645
                     /locus_tag="PKX09_02085"
     CDS             66975..>67645
                     /locus_tag="PKX09_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOU16644.1"
                     /translation="MSILVTGSAGFIGFHLARQLLQEGIEVVGYDNVNDYYDPSLKEA
                     RLRILNDFDKFKFYRADLCDFATLQKVFSDQTIEKVVHLAAQAGVRYSLINPFAYQKS
                     NLEGFLNIIELSKRAKVKNFIYASSSSVYGNNRHLPFSVQDNVDRPISLYAATKKSNE
                     LIAHVYAHLYQLPCTGLRFFTVYGPYGRPDMALFIFTKNILEGKPIDVYNFGQMKRDF
                     TYIDD"
BASE COUNT        18581 a        15657 c        13485 g        19922 t
ORIGIN      
        1 gtacgcagac acttggcatt atcaacgtag tcggttctac tatcgcgcgc gaagtagatt
       61 ccggcattta cttacacgcc ggaccggaaa tcggtgtcgc cagtaccaaa gcttttattt
      121 gccaggtcat taccctactt ctactggcac tctatatcaa acaacaaaag ggagaattaa
      181 ctgatccggc tcttttagcc aagatagata tgattccgga acaaatccgc caggtagccg
      241 aacattctga caatctgcgg gaaattgcat cggcttatta tctgaatcgc aattttatct
      301 atcttggacg tggtatcaat tatcccgtcg ctttggaagg ggcattgaaa ctcaaagaaa
      361 tttcatacat tcatgccgaa ggttacccag ccgccgaaat gaaacatgga ccaattgctt
      421 tgattgacga aaatatgcca gtcgttttca tcgctacgaa gggggctgtt ttcgataaag
      481 ttctcagtaa tatgcaggaa gtcaaagccc gcggcgggca aattttaacc atcaccgacc
      541 ataataaccc acatctgcaa gaactctcca atcatctgat aattgtgccg gagaccagcg
      601 aagacctttc gccgctcatc aatgtcatac cgctccaatt actagcctac cacatcgctg
      661 atctgaaagg cttaaatgtg gataagcctc gtaatctggc aaaatcggtc acagttgagt
      721 agctgaaata gaaataaaac ttagctaaca aggagcgtta gcatttgcct cgtctcgaaa
      781 aaaactgcaa caattctcct tttaatttct atactttttt taaattaact gagttttaag
      841 atagcggaga aaattatgtt aaaagaaata agtctgggag taattttaat gttcagcctg
      901 tgggtaaccg gtcagacggc tgagccgttt tttccttata aatataatgt ggaagtttta
      961 ccaaacggct tgaaagtcat tactattcca atggaaagca acgggatcgt ggcttattat
     1021 tccgtcaccc gcaccggcag tcgggacgaa tgggaaccgg gacattccgg attcgcccat
     1081 ttgtttgaac atctgatgtt ccgcggcacc gagaaatatc ccggctcgct ttatgataaa
     1141 actataactg aattaggtgc cgacgctaat gcttatacca ctgacgatta cacctgttat
     1201 catctgaact ttgccgcgga agacctggaa aaaatcatcg aaatggaaag cgatcgcttc
     1261 atcaatttag cttataagga ggaagaattt aagaccgaaa ccggagccgt ttacggtgaa
     1321 tttctcaaag ggcgagtcag tccgtggttc gtcttgtacg aaaagcttta cgatgtcgct
     1381 ttcgacaagc acacctacaa acacactacc attggatttg aagccgatgt gaaagctatg
     1441 cctacaatgt acaaatacag taaaactttt ttccaacgtt actaccgacc ggagaatgtc
     1501 gttctgctca tcgtcggtca gatcgagacc gagaaaactc tcgaacttgt tcgtaaatat
     1561 tacggcgatt ggcaacccgg ctatgtctct ccccaaatcg aacccgaacc gcctcaaact
     1621 gccgagcgca aggcggaagt aatttatccc gggaaaaccc tgccaatcgt catggtcgct
     1681 tacaaaagtg atgcctatga tcctaataat cgtctatatg tagccggtct tctgctagag
     1741 caattagctt tcggagaaaa tagcgacctt tacaagcgat tggttttaaa agaacagaaa
     1801 gtcgagttca tcacagccga cttttcggtc agtcgtgatc ctaaattgaa cgaaatttac
     1861 gctatggtaa aatcgccgga cgatattgac tacgtccgcg aacagatttt tatgacaatg
     1921 gaacaattca agactgaagc cgtccctgag tctcgcctcg aggatctcaa aaaacatttg
     1981 aaatacagct ttctgatgaa tctggacact cctgataacg tggcgggcaa tctagctaat
     2041 tacgttgcca tcacaagtgg aattgaaacc gttgatcaac tatttaacca aataatgaat
     2101 gtcaccgtca ccgatattca gaaagccgct cagtattatg gcgttcccga aaaaagaaca
     2161 gtagtcattc ttaaaggagt cgcgcaatga aaaccaaatt aaatctactt ttaagctggg
     2221 gaattatggc aagcatttta ctttcaaatt gtggttctaa aatcgacgtt tctcgttgtg
     2281 tgctcctgcc ggtagccgac gatccgacta tctcttaccg catttatttc aaagtcggct
     2341 cccaggatga tcccgccggt aaagagggat tggcgttcct gactgctcat atgttaagtg
     2401 aagccgctac tcaaagtaat tcttacgaag aaataattgc gaaattattt ccactagcgg
     2461 cgtcctacga cgctacggtt tcggcagaga tgaccgtgat ttccggccgc gtgcaccgag
     2521 acaatctccc ggcttattac ccgcttttta ccgaagcgat cctcaaaccg gctttccaag
     2581 aagatgacct gaatcgtctg aaaagcaatg tcttgaatta tcttgaaaat gttttgaaat
     2641 acgctaatga cgaagaactc ggcaaagcgg tgctttataa tgaaatcttc gccggaaccg
     2701 gctacggaca tcccaccgag ggattaatca acagcattaa ttccattacg atcaacgacg
     2761 tcaaagattt ctatcaaaaa atgtataacc gcaacaatgt tatcatcgga ctgggtggcg
     2821 gatacgataa tgaaacgtta accaaattgc agaaagacct ggcgcgactt cctcgtggcg
     2881 tggaaaatat cgcaactcga gccgttccct acgacattca gggcttaaaa gttaccatcg
     2941 ttgaaaagga cgctaatgct actgctatta gtcttggttt tccaatcaat atcacacgag
     3001 aaaacccaga atggtatgcc ctggcagtcg ccaattcctg gttcggcgag caccgcaatt
     3061 ccagtagtca cctctaccaa gtcatccgcg aaacccgcgg cttgaattat ggcgattaca
     3121 gctatatcga gcattttccc aatagcggct attaccaatt tccaccggtc aacgtcgccc
     3181 gccatcaaca aatattcgaa atttggattc ggccggtacc gaacgaaacc cgccatttcg
     3241 tcctaagagc aactatgcgt gaattacata atttcattga aaacggacta acgccagagc
     3301 aattcaatct cacccgcaat ttcctacgca agtatattct acattatgcc ccgacgacaa
     3361 tgatgcgcct cggctatgca attgatgaca aattctatgg aataaaagga agtcatctgg
     3421 aaaatttccg cgtcgcactc gacaatatga ccgttaacga cgtcaatagc gccattcgca
     3481 aatatctgca atatgataat cttcaaatcg tgatcgtcac caacgaagcc gaaaaattac
     3541 gcgaagactt gcttgctaat acacccagcc cgattaaata tgttaccccg aagcccgatg
     3601 tagtgcttaa agaagacctc gagattgcaa actacccact tgctatccaa cccgaaaata
     3661 ttaaaatcgt aaacattcgg gatttattct atgaataatt ccgccaaaaa tttcacagat
     3721 tcacgcacta aagatgtcaa gcaatgcttc gacatctcga ccgaattgcg actcgataat
     3781 aaatactatc gcaaattcgt actgactttt ttcgacaatc tactggattt tgacatcagc
     3841 agtaaggacg catctttaca accggcgaaa tattatcaac gccaggtaac cgccattatc
     3901 aaagccaaag cgccggcaat tattgccgga atggcggaaa tacaggcttt tctcgatcat
     3961 cataatctac taagcacttc agattttaaa gatggcgatc cggtcaaagg cggcgaaacg
     4021 attatgacta tcgaaggccg ggctgatcgc attctgacgg tcgagcgcac cgtcttgaac
     4081 attttccaac gattgtccgg aattgccacc gttacagccg aatatagcca ggcgctcaaa
     4141 ggtctgccct gttttgtaac tgccacccgt aaaactcttt ggggaatcgt agataaaagc
     4201 gcactgcaat acggcggcgg attaactcac cgactcggac tggcagacgc cgccatctta
     4261 aaagaaaatc acttaacggt tctaaaaaat tccggtgatc cgcaagcact tatgactgcc
     4321 ttagacgcta ttgtagccac tgacccgttt ttgcgattcg tcgaagtcga ggtcactaac
     4381 gacgatgaat tctggcaagt tgttgaggct tataaccgct taaggccaga aacctactgc
     4441 gtgataatgt tcgatcattt tgcgccggaa aatatcgcgc aactgattaa ggcaacgaaa
     4501 aataatgggg tttatccaac aattttcttc gaggcatccg gcaatataac actcgagaca
     4561 attcgtgatt acgccgccag cggcgtggac gttatctcgg ttggggcatt gactcactca
     4621 gtaaaaagcg ccgacttcag tctgctgatt aataaattct aatccaaaac agcccggtcc
     4681 gacgatttta tcgtgattcg cctatcattt ttcccatttt aatccgtttt agtttttatt
     4741 tctatatcaa ccataattaa ttttgctccg atgaaaccga tttatcttga ttataacgct
     4801 agcacccctc tcctaccgga agtggtggac gctatgttac catttctgaa aaatgtcttc
     4861 ggcaatccat ccagcagtca tttctacgga caggaaagca aacaggccat cgaaaatgct
     4921 cgccgtcagg tcgcggaaat gttgaattgc cagcctgaag aaatcgtctt caccagcggc
     4981 ggcagtgaat ccaataactt cgctattcgc ggaatagccg aaatgcatcc cggcggccac
     5041 atcattacca gcactattga acacccagca gtcctggaag tctgtcgcta cctggaaagt
     5101 aaaaattact ccgttactta cttaccggtc aatagcgatg gtttggtcga attgcctgcc
     5161 gttcaagccg ccattcgtcc cgacactttc ctaattacta ttatgcttgc caataacgaa
     5221 atcggtacga ttcagccgat tcgcgaaatc gccgctctcg cccataaaca cggaatttta
     5281 gtccataccg atgccgctca ggcggtcggg aaaatagtcg ttgatacacg cgagctggat
     5341 gtggatttac tctcagtcgc tggacataaa atgtacgctc ccaaaggtgt cggtgccctt
     5401 ttcatacgaa ccggcactga aatcgctcca ttgatttatg gcgctggaca tgaacgtggg
     5461 ttacgacccg gcaccgaaaa tgtactggaa atcgtcggct tgggtaaagc ggctgaaata
     5521 ttccgagcga aatcggctga aattgtgccc cgacaaaagg cgcttggtga aaaattcttc
     5581 aacgatttac gaactgcaat tcccgaactt ggcttaaacg gtcatccaac ccaaaaactc
     5641 gccaatactt tcaatgttta ttttccggga atagatgcca ataccatgct ggcggaattg
     5701 acgcaagttg cggcttcggc tggcgccgcc tgtcatgcca gtagcattac gccttcgcat
     5761 gttttaaaag cgcttggctt gccgaatgaa cggattctcg gaagtgtccg cttctcgctg
     5821 ggacgtttta cgaccgaaga tgaaatcgcg gatgccgtat cggcaatcag tcaaacttac
     5881 catcgtctaa tcggcgcaga ggaaatatct gtccccatca catttgacga accaatccgg
     5941 ttaacgcaat tcacacacgg tctcggttgc gcctgcaaaa ttcgtcccca aactctcgaa
     6001 aaaattctac gtgaaatcag accacggatg gcacttaacg tgttagttga cttctcagct
     6061 tctgacgacg ccgccatata taaaattaat gacgagcaag cgatcgtagc aaccgttgat
     6121 tttttcactc caattgtgga tgatccatac gatttcggga gaatttcagc ggctaactcg
     6181 ttgagtgacg tttatgcgat gggcgctgag ccactatttg ccttaaatgt agtagccttc
     6241 cctgaaaaac gcctgccggt agaaattttg actcagattt tgcgcggcgc acaggttatc
     6301 gccgatcagg ctggtatccc gattctcggc ggtcataccg tcgaagataa cgaaccaaaa
     6361 ttcggtctgg tggcaattgg cattattcat cctgaaaaaa ttatccgaaa tatcggcgcg
     6421 caacccggtg atgctttaat tctgaccaaa ccgatcggcg tcgggattct ttcgacggct
     6481 ctcaagcgcg gtttattaga tgaactggcg aaaaatctga tcatcgccaa tatgaccgaa
     6541 ctgaacgcca ccgccgcaga agtgatgaag tcatttccgg tgcacgcctg cactgacatt
     6601 accggatttg gtcttttggg acatttacat gaaatgacca ccggcagtcg tgtccgagcc
     6661 cagatttcag cctcgtctgt accgatttta ccgtccacta acgagatggt tttacgcaat
     6721 gttattccgg gcggcaccga ggcgaactta tcgtttattt caccgatggt catctgggat
     6781 aaaatgataa cggatagcga taaaataatt ttgtgcgatg ctcagacttc tggtggcttg
     6841 ctaattgctg ttgcggatga atttgccaat gatatgctgg cacaattaca tcaagcaggt
     6901 gtaataaatg cggctaaaat cggcaaaatt gagtccggtg ctgatggaaa aattaaggtt
     6961 gttccttagt aaaaattttt tagattagca aagttatgac ccgttttcgt aaagaatact
     7021 caaaaattgc ccggaagctg acaagcgccg accttcaatc cggaatagga aacagtgaat
     7081 taagtctcga taatattgtt agtgatggta gattagctgt ggactcgcaa tttttcatgg
     7141 gcgttttcag ccgacagggt ttgctaaatg ccattcgggc tttcggactt ttggatattc
     7201 tcgagcgaaa aggattgacg aatattacaa tagacattga caccagtgat ccgtatatcc
     7261 atcgccttta tgcctatagc ggaaagccgc taaacgaaaa taaaatctgt gaattagtat
     7321 taaaacaagg tccgcttcat tttgaaaata agccactatc gtctttcccc cgtagaactg
     7381 ccaatatatt atatatagaa tggcttctac tccaaaatcc acgcgaaaaa ttttcacccg
     7441 aaaaaccgcc cttgcccggc cagaattatc ccggccttgg tattggcgat cgcctaatgg
     7501 aaattatgat acttatgacc cgtcgcctta acttggaagg tattatcaat aaaccgcatt
     7561 actttcatac agcgtttatg ttcagtaaag agttcgtgtt catcaatccc tataatcagg
     7621 ctttgatgta tgcaatcagt aaagacttat tggctaaata ctcattctgg atggtagctt
     7681 gggcggcgca tttcaactgt attattaata aaaatgacgg caaaccaatg gattggaaga
     7741 caggagacct tatcctgccc ttcaccaaag atttgattaa atactttcgc gggcgcgaat
     7801 atcagcagga ggttagccga cttatagcta atttctcctt cgaaatcgat cttgataaat
     7861 tacataccgc ccttaaaagc aatgaccttg aaaaatacgc tcgatttcct aaataatttt
     7921 ctgaattaaa ttgactccca acaccccaat aaattttcaa tcaccgacgg ctttagtcct
     7981 gggaaatgga aattacccaa actggtttga tatcaaacaa tttcaagtgg aatataaagt
     8041 gctattggtt tgcgccgacg gcggagcgga aaaagcacgc caattgaatt taatacccga
     8101 tttgataatt ggcgatatgg attctgttac cacggaaacc ataaatcatt ttcaagccag
     8161 cgaggtaaag atcattcacc aaccaaatca gaataacaat gatttggaaa aatgcctgcg
     8221 ctggcttttg cgacatggct ggcggcagtt cgtattagtt ggatttaccg gaaggcgcga
     8281 tgatcaaact atcgcgactt tacagataat gagaaagtat gcccggcgag cgcagtttct
     8341 gttatttacc gagacagcac aaatttattt attgaaaaaa ggcatctggc tcattaacgc
     8401 cattcccggc cagaccattt ctctgttcgg ttttccaaga gtttatcatc taactactcg
     8461 gggccttcaa tatccaacca aggacgaaaa tttaacaggt ggttcgcacg gtctaagtaa
     8521 taccgccatt gatgaaatgg ttcaaattca attttccagt ggttatttat tagttgtcgc
     8581 gattcaggag ctaaattgac gtctcttaga ttggctccga ttgatctgtt aattatcgcc
     8641 atttatctgg tcggcatatt tctaattgga ttaaaatact cccggaatta tcgaggaaat
     8701 gttgacgact atttattagc tgggcgcagg ctttcattac cggcgttcgt cgccactctg
     8761 gtgtcaacct ggtatggcgg catcctgggg gtcggtgaat tcacttaccg ttacggatta
     8821 ttgaattggg tgactcaggg attgccgtat tacttctttg ctattctttt cggaattttt
     8881 cttgcacctc ggattcaacg cagtggtctt tacacgattc ccgatcagct ttaccagcgt
     8941 tatggcaaac cggctggatt tctgggtagt gtgtttgttt ttttgttgac taatcccgct
     9001 tcgcatttat tgatgagtac gcttttactc agcgctattt tcgggatttc tttttggttg
     9061 gcttttctga ttggagttat tttttccata ttctatgttt ttatgggcgg aatgaaatct
     9121 gatgttttga cggatttctt tcaatttggg ctaatgtttt taggatttgg agtgttggca
     9181 gtaaaactca taactaacta tggcgggttg aatttcctgc aggcaaatct gccgcaaaat
     9241 cacctcagat tcactggtgg cgaaaactgg cagtatatta tggtttggtt ttttatcgcc
     9301 ttttggactt ttgttgaccc gggtttttat cagaagtgtt atgccgctcg cacgccgcag
     9361 atagcgcgcc gcggagtatt gatttcagtg attttctggt tactttttga tatgctcacg
     9421 acaacctgcg gtttatatgc gcgggctctt attcctgata taaatccttt aatggctttc
     9481 cccaagttag gcgaaatggc actgggagat ttttggcgag gtatattttt tattggtttg
     9541 ctggcaacga ttatgtccac tttggattct gttgccctgc tctcagctat gacttttgga
     9601 cgggaccttt tgtggcgtct gaaacccaat agcaatatta accgcaacac ccaaatcgga
     9661 ttctttgtta tcattggctt atctatattg gttattcttt tccttccttc ggtagtaaaa
     9721 atctggtaca cttttgggac attatttatc ccagctctgt taatccctgt catttgtaac
     9781 tttacgaagt ataattataa gtcgtctgtt atgttcaccg gcatgctggg atcatttttc
     9841 gcaacattaa tctggttcat aattggaatg agaaatggtt caataatgga acccatttat
     9901 cttttcaaca tacagccctt ttttattgga ttattgtcct ggttgctctt tttgcccttt
     9961 ttagttgata ggtttactac ctaaactgac cgacataata attcaataaa ctgaaaaaat
    10021 attgatgcaa atctgttgta ttaatcagtc agattagcta aattttgttg cttttttaaa
    10081 atgtccggtt aatttaactg ggcgattagc tcagttggtc agagcgtccc gttcacatcg
    10141 gggaggccat ccgttcaagt cggatatcgc ccacgtgata ggttgggttt tgaaagaatt
    10201 aattgtccga tacaatacat tacaccatat aaaatgcacc gcaagcggtg catttttttt
    10261 ataaaaacaa ctggaggtta aatgtttaaa gaactcgaaa gattggttga agttcaaaaa
    10321 atcgatatca aattgctcga aatcaataat cttagaggcg atttgccgca aattgtcgag
    10381 aatctccaag cccaaataga ttccttatcc aaagagctgg aaaacgaacg taagcgcgaa
    10441 caagaaatta cgcgggagat aaaagaatta gaaagtaaaa ttgccgatga tcgcattctt
    10501 ttaaatagat atcaagatca actttactta gtcacttcta ataaagaata tgatgctctg
    10561 acttccgaaa ttgacaacct caagcaagag attgacaatg ccgaatacaa catcttgaaa
    10621 ctttccgatg aaatcgaacg cctgaagatt tcgattaaaa gtaaagaact aacccttgag
    10681 gaaaaaacca gggaattgga tcaaaaacag tccctattgg acaaaactga tattactact
    10741 aaagacgctc aggaggcttt actggaacaa aggaagcgga ttgtagattt aatcccaatc
    10801 cgctatatgc gagaatacga acgtattgct aaagccaaaa acggaattgc gattgtccca
    10861 attctgcaga tttttgaaga aaaagtagat aaaaagggaa atgttgaata tattccaatg
    10921 caggtttcct gtggcggatg ttcgaaaata gtgccgccac agaagatcct tgaaatacga
    10981 agtggtgacc aaattaatcg ctgtgaattc tgcggccgca ttttatactg ggatgagcaa
    11041 agcagtgaaa ttcgttccaa ttatgaagag gagcttttct gaagttggtc agacagccgc
    11101 tccgatttta tcggagagga aagtccgaac tccgcagggc agggtgtccc gtaatccggg
    11161 atcattccga atgatcggaa tgcaggaaag tgccacagaa aatataccgt cccgccccgg
    11221 cgggataagg gtgaaaaggt gcggtccgac tgaggtcggg attaaagtaa gagcgcaccg
    11281 tcccgtctgc aaaggcggga gccaggcaaa ccccacccgg agcaagagca agcagggaaa
    11341 aggaatagcc cgttccgttt gattcccggg tagctcgctt aatcccgact gaaaagtcgt
    11401 ggatagataa atggctgtcg tctcgattcg tcgggataca gaattcggct tatagaccaa
    11461 cttcagaaat tttatatcat gaaacaaaaa tggcttatca gcaatccgaa tcccaacgct
    11521 gtccaccaac tagcaggtga gctccagata ccaccggcta ttgtgcggat tttacttaat
    11581 cgcggtctgt ttgataagga ttcaattgaa aaatttttta gtccttctac tcaagatacc
    11641 catgatccct ttttaatggc ggatatgacc aaagccgttg aacgactcgg tcaagccatc
    11701 cttcaacgcg aaaaagtctt gattttcggc gattacgacg tggatggcac cacagccacc
    11761 tcccttcttt atctggtttt ccatgaaatt gggctcgact ccgcatttta tattcccgac
    11821 agagaacggg aagggtatgg tctctcccaa ctcggcatcg aatatgccag acaaatcgga
    11881 gcaactttga ttatcacctg tgattgcgga atcaacgcct tcgatacaat tgattatgct
    11941 aattccaacc agattgatgt gatcgttacc gatcatcacg aaccagctga aaagcttccc
    12001 gccgccttgg ctattctaga tccaaagcgc gaagatgacc attatccatt taaagaatta
    12061 tccggagttg gcgtcgcttt caaattctta cagggatttc atattgcaaa taatctacca
    12121 gtcaccaacc tttatcgtca tcttgattta gtggcaatcg gaactgccgc cgacatcgtt
    12181 ccaatcctcg gcgaaaaccg tgttttagtc gccaaaggtt tggaatatct caacagttcc
    12241 caaaaagttg gcttacaagc tcttctcaaa acctccggct tcgatagcaa aacaatgaac
    12301 gttgtcaata tcgttttcgg tcttgccccc agacttaatg ccgccggacg actcggggaa
    12361 gcctctcgag cagtacactt gctgacatcc ttcgaccgca aagaagcatt tgagctagcc
    12421 aacctccttg aacaagaaaa ccgcaatcgc caagctatcg aacgtgggac gatcgacagc
    12481 gctattttac aacttaacgc cactcatgat ttagatgccg acaagattct gattctgagc
    12541 aacacaaatt ggcatcatgg tgtcatcggt atcgtcgcct caaaattaaa ggaattatac
    12601 aaccgaccgg ttgtcatgat ctcgttccag aatggaatcg gcaagggctc tgcccgcagt
    12661 attcccggct ttgatctata tagcgccttc aactcctgcg ctgacttgct ggaaaattac
    12721 ggcggacata aaatggcggc tgggctgacc attttgatcg aatcactacc ggaattcacc
    12781 agacgcttga aaaaaatcgc cgcagaacag attgacgaac agatgcttaa ttccggattg
    12841 caaattgaaa gcgaaattga ctttaatgac attaatcccc aaacactctc ctttctccat
    12901 aaaatggctc ctttcgggcc cggaaatatg cgaccggtat tctgcacccg taacgtaacc
    12961 gtctccggaa tgccgcgcat aattagtgaa aatcacttaa aattcaaagc ggctcaaaat
    13021 cgttcggttt taagtgctat cggctggaaa atgggttgct attacgaaat gttaatcagc
    13081 aatcgccccc ttgatattgc ctttgtaatt gatgaaaatg aatggaatgg ctataaagaa
    13141 attcaactta atatcaaaga tatcgtatat tctgactacc attaaaaaat gacaggagaa
    13201 gctgtaccaa tgtacaaaag taaaatcgcc agcctcggga aaaatgttcc cgaaaatatt
    13261 gtaactaatg ctgaccttga aaaactgatg gataccagtg acgaatggat cgtcgaacga
    13321 accggtattc gcgaacgacg ttttgtaacc ccaccctgcg gcgccagcca gctggctcta
    13381 ccggcggtac tggaagcgat taccagtgcc ggattaacta aaaatgacat tgatttaatt
    13441 atcgttgcga caactactcc ggaacatctg actcctggta ccagcagtta tttacaggct
    13501 ttacttgaat tacccaatat tccgacactc gacattcggg tgcagtgcag tggttttata
    13561 tatggacttg cgatcgctga cgcttttatc aaaaccgggc aatataggaa tatcctcctc
    13621 gtcggagtcg aagttcagtc cgttgggctg aatatgacca ctgccgggcg tgacatcgcc
    13681 gtcctctttg gcgacggagc cggcgcggct gttattacgc gttccagtga tgaaagcgca
    13741 attctctcta ctcatttatt cggcgatggc cgctatgcga atgaactctg ggtagaagct
    13801 cccgacggtt caaaaagacc gcgtctttcc aaagagatgc tggacgaagg gaaacactat
    13861 cctaaaatga acggccgtca agtttttaaa catgccgtta ctaaatttcc tgaagtaatc
    13921 aacatcgccc tcgaagccaa caatctgaat aaaagtgata tcacattaat tattcctcat
    13981 caggctaatt tgcgtattac cgaagccgtt gcccagcgcc tggaaattcc aatgagccaa
    14041 gtctatagta atatccataa atacggcaat acaaccgcgg cttcgattcc tctggcaatg
    14101 tatgacgcac ttcaagaagg cagatataaa aaaggtgatt atctaatcct ggccgcattc
    14161 ggttctggat ttacctgggc ttccgccgct ctacgctggt agtaaatatt ttaaattatg
    14221 agcataaatc gtggcgaatt ctaattcatc taaaatagcc ggcaaagagc gtggcgtttt
    14281 ccaatccagg cgccagcgca ccggtgtatt tttaatctgc ggagtaatcg tcgtagcctt
    14341 agctttactc tttcccaaaa ctcattacaa catcactaag tataaaataa atgacattgc
    14401 acgctcatca ataattgcgc cgttcgactt cccaatcttg aaaacagaac aggaattaga
    14461 agccgatcgt ttagaagcca tcaaaaaagt tccgttcgtt tttattcagg ataaagacgt
    14521 cgaaaaaacc gaactaatga acctccagaa attttttacg gaaattgatc gcttacgatt
    14581 agcccgaatc aagtatgatg aatctctgcg aaatattagt attaatcgga gcgaagaatc
    14641 gctggcaact ttgacagctg attcaattac tttttttgct ttacagaaaa aattcctcaa
    14701 cgattataag cttaataaaa attcaccagt tctaaagtcc ctggtatatc agtacgatcc
    14761 caagaatgcc aaccagctct cggctcatgt ccagaatcag gtaaaatcag tgcttagcga
    14821 actatatgtt ttacagattc tcgatattcc caaatcccag ataatttcgg aaaaaattgc
    14881 catccttcag ggcggcgaag aggttttaga aaatccaaac gaggttttag aattatccga
    14941 agcctggaca aaagccaaat tggctttgca agcccgccta aatgactaca ccccagaagt
    15001 ggttaatgcc gcttacgaca tcgtggtcgc tttcctgaaa cctaacctca tttaccagaa
    15061 acaaattacc gagaaacggc aagcggaagc cattgcgaaa gtccccatca gcaagggagt
    15121 tgttcttaaa aacgaaaaaa tcgttgacgc taacacgcgc attacgccgg aaatctatca
    15181 gaaaattgaa tcaatggcta aagaacaagc gcgcttagcc aatgtgcaac gcagttggcg
    15241 gcgagcttta ccggtaatcg gcgatccggc aatattcatc tgccaggtcg cggtggttgc
    15301 gattattttt acctttttta tgactttttt atttgcttat cgcaccgata ttatcaccag
    15361 tcccaagaaa atgtttttaa ttgggatcat cttcatattg caagcggttt ttgcggctat
    15421 actggtcaat cattttaaaa tttctgaata tacgattccc attactctgg cggctatgtc
    15481 attgacaatt ctttttgaca gtcgcgtagc tttcgtcggc actgctacca tctgcttgat
    15541 aatcggcgcc caaatcggtg gcaatttcaa ctttataatc gtgtcggttt tcgtgagcac
    15601 ttttgcgatt tatgcggtcc ggcaattgcg gaagcgtagc caaattttca aatctatcat
    15661 ctatatcgta ctcgggtatt gcctggctat tttcgccacc gaaatgttga aatactcgcc
    15721 cccgaaatct atcctaaata atattttcta cgccacgatt aatggcgttt tcgcaccgtt
    15781 cctagcgtat ggaattatcg ggctattgga aaaccttttt ggaataacca ctgatttatc
    15841 actactcgaa ctggcggatt ttaatcaacc gctcctaaaa ttactttcgc acgaggcaac
    15901 cggaactttt actcatagtg taacggtcgg caatctggct gaagccgccg ctgatgctat
    15961 tggagcaaat gccctattgg ctcgcgtcgg ttcctactac catgacattg gcaagttgac
    16021 taaacccgaa tattttatcg aaaaccaggc ttttgattat aaccggcacg ataaactggc
    16081 gcccaatctt agcgcgatta tcatccttaa ccatgtcaag gagggtctgc ggctggcaaa
    16141 cgaatataaa ttaccccagg ttattcagga cttcatttct actcaccatg gtactactcg
    16201 cgtcgaatat ttttacgata aagccctcaa aaaatccgcc aagcccgacg aaatcaatga
    16261 tgccgatttt cgctatcccg gcccaaaacc gcatactaag gaaaccggca tcgttatgat
    16321 ttgtgaaaca atcgaagcgg tatgtcgctc cctcaacaag ccaactatgt cggctatcga
    16381 aaaagtcgtg gattcggtga ttgaaagccg actcaaagaa ggtcaactcg atgaatgccc
    16441 gctgacttta gccgatctaa agaaaatcaa aggcgatctc aaggaaggta ccggaatact
    16501 gccaatttta cggggcattc atcacctgcg tgtggaatat cccggacagg aaaaattctc
    16561 caattcttcc ttcccaagta acaaaaaggg aaaaaatgac ggtagtcaaa atctttaaca
    16621 accaccctca ttacaccctt ttaaaaccgg caatcgaaca ggttgtctct tacattgtca
    16681 atatggagca acaagccgtt cagagcatca ccttcatagc tagcgatgat aaaactctta
    16741 atgaaatgaa agtcaaatta ttcgggatcg atcaactgac cgatacaatc agcatcaatt
    16801 acaatgagat agaccaaccg gtagaaggcg aaatttatct cagtctcgat cgcattgagg
    16861 acaacgccca tacctttaag gtctcctttg aaaaagagct cgccctggta attattcaca
    16921 gcgttttaca tttactcggt tacaatgatg acaacctaaa cgacaaaaaa caaatgcaag
    16981 ctcttcagaa tttttatatc cagcaaatta aattccctcg cctttaccgt cagcgccgaa
    17041 tccagcctaa ttatgaatga tgaactttca cgctattttg atgaactaat cgagatcgtg
    17101 aaaaccttgc gcggtcccaa tggttgtgaa tgggacaaag ctcagactcg tgattctctt
    17161 acgccttatt tgctcgaaga agcccacgag ctcattgact ccatttctga aaataacccc
    17221 tcgaaaataa aagaagaact cggcgatgta ctgctccata taatttttca ggctcagatc
    17281 gccgccgaag ccggtgaatt caccattgcc gacgctttgc aacaaatcaa cgccaagtta
    17341 attcgacgtc atccgcacgt tttcggcgaa actaaagttg aaaatgtcaa cgaaatcaag
    17401 aaaaattggg aagaaattaa gctgaaagag gggcgccaat cccttatgga tggtttaccc
    17461 aaatccctgc ctgctttact acaagcccgc cgcgtccagg aaagggcggc tcaagtgggt
    17521 ttcgactggt ctaccatcga accaatctgg gctaaaatca ccgaggaaac gcgtgaattg
    17581 caagaagcca ttgctactcg taagaaagat gagatcatag cagaattcgg cgatttactt
    17641 tttacaatgg taaatctttc ccgcttcctc gatattgatc cggaagaagc cctgcgacaa
    17701 acagtcacta aatttatccg gcgttttcag gctatcgaaa atgaattagc cgccaccggt
    17761 aaaaatttca aagatacttc gctggaagag ctggataaaa tctggaataa atcgaaagat
    17821 aaagaatcct gaatattaag gccactaact ctcggtttag taaaaaatca ttcccacaat
    17881 ataatgaaat ttttcgccga acctattgac gaatatctta attaatgcta tattgtttat
    17941 gtatgaaacg cttattgaaa aattatcctg caatagtgtg gcgagtgttt ttggaaccaa
    18001 caccataaac ttgggagccg gactccgaac ggctaaagca cgcttcctcg ttgaagaaac
    18061 ttgatccgtt cgggaggtgc ccaccgtgat tttcacggtt cccttaatcc gccagcgctg
    18121 ttgcaggata tttttatgta acgatactca aaccaggaat cgagttcaat tccgtccgga
    18181 actcatccgc tttctgaact tcaatttgca ccgtaactat gacgctattt tcggtaaatt
    18241 ccggcttgct cgattccgaa aaacgcccga gaaaatgtgc cagctgtgag tagttcgcta
    18301 gctggcattc gattcttagg gtttggcgct ggataaaagg tttgtgttcg acaagatcaa
    18361 gcgtcttccc ggccgtagcg ccgtatgccc taatcaagcc tcctattccc aacttcgtcc
    18421 caccgaaata tcgcacgact acgacaagcg ctttgaccaa ctgccggctg gttagcgctt
    18481 ttaagattgg cgctccggct gaaccgcgtg gctcgcccgc atcgtgagca taatatagca
    18541 attgattatt ttcataaatt cgatacgccc agcaaatgtg agtagctttt cggtgcttct
    18601 ggctaatttc cgctattttt tggactgctt cggacttatc ggcaaccgga aaacaatacc
    18661 cgacaaactt tgaatctttg actgttacca taatttcagc cgaatcagca attgttttta
    18721 gcccttgcag taacatttca gccataaatt ttaaatttac ttcagtagta aaattttgcg
    18781 cgttagattc ttgcttccga tcgcaatttg cataatatag acaccggatg caagttgcgc
    18841 ctgtcttaat tcatcactcg agaatgataa tgaataatta ccaatctcga tcagtccatt
    18901 atcaaaattc ttctgaaata catttcgtcc cgtaaggtca aaccagttcg tcttgattga
    18961 attaacaatc tgacccacct gtagatccac tatcaaatct cccgattttc ccggagaata
    19021 cggatttgga tagacgtttt taatagttga cgctattcga aattcatcac ccaccttgga
    19081 aattgtcaca gtcagattag gagttgtagt tttactgcta ttagtcataa aaataagtag
    19141 agaatcatta ttggcggcat taccgaccaa taaattttga ttaagtgaca atgtctcggc
    19201 tatttttctg acccgatttt taatgaacaa gtaacgtcct agaaaattct ccggcgttga
    19261 tgaattgaca tttattccgc ataaccaatt ctggtcgaac actacttgat atggcacaca
    19321 cgcaaagggt tctattgatt gattaaaaac cgcactttct gtaaagacaa ctttgttaat
    19381 atccggaatt tttaacaatg gaaattgcat agcatcgctg gaaagcgccc aatcgggatc
    19441 atagcgttct ccggaaaaat agcatcggag ataaaactca ttccacaaat cggctaaatc
    19501 ggcattataa tcatccgcca gaaccgcctg aagtgattcg aaagcataat tagtcttgat
    19561 ctcctcccag actttgacca agagattctc gccataacga tctaagataa atctaagaaa
    19621 aaaacctaac gcataggcat aatcataatt ccaaagcgat ttaagtggag aattgaaaaa
    19681 agcgccgaca taaaagagat aatcgttcac ttccggataa gcccgttctt cgaaccaggt
    19741 cgagcaccat tccaggaaat aatcatcggc gcttgtccgc ccaccacccg aatttgccaa
    19801 gcggttacct tcccaccaat tgtaaccgag ctgtactacg tgaaaaaatt catgggcaac
    19861 tgtgactcgc agaccattta agcccgttgt cagatatcca acaaagtcat tatcaattac
    19921 cgtataggcg gtatagtcat aggggcgatc agttgtctga accggatttt catcgtaggt
    19981 ataagcatag gcagccccac ccagattaat aatgtagata tcggtttctg gactaaatat
    20041 atcatcaacc ggcggtgatt gaaaaccaag tgtatcaatt agcaaactat aagaccgctc
    20101 ggcggcctgg gcagtttcat aaacaaaatc cggcacatcg tcagtatttg taatcgtccc
    20161 atcaacggca tcgtagcctt ccaatgtgta atgaatcaag aaatggcttc ccgctgactg
    20221 ataacttaaa ggcaaagacg gcctcgagta tttatacccg accaccggct gtttgagcaa
    20281 ttcgcgcgga ggtctcagat catttttgac ggcgttaagc gcttgccagt agctttcatc
    20341 ggtagcaatc aattcaattt gatcaattgc cagggcttgt tcgaccacaa tcagacatac
    20401 gattgtaaaa gttaattgac ggactatttt catttattcc ggaaggaaat tatcagcggc
    20461 actccagaaa aaccgaaccg gcggcgcagt tgattttcta aaaagcgtcg gtattcttct
    20521 ttgattaagc gcggctcatt acagaaaaaa acaaaaaccg gcggctcagt tttgacctgg
    20581 gtgatgtatt ttatcttgat aaatttgccc agatttgccg gaggcggtgt gcggtcaata
    20641 acgtcctgca ggaattggtt caattcactt gttccaatgc gcattttgcg attctcatag
    20701 accatccggg cagtttctaa aattttatgc acgcgctggt gagttttagc tgaaataaaa
    20761 attatcggat aatgttccag ttcccgaaat tgttcgatca aattacgttt gaaattaacc
    20821 tgggtattag tttcgcgctg aaccaaatcc cacttattaa tcgcaattac caagcctttt
    20881 ctcaagtcaa tgacctggcg gacgatggcc gcatcctgac gggtaaatcc tttaactgtg
    20941 tcaacaacta ccagcgccac attacagcgc tcgattgccc gataagcacg cagaagacta
    21001 taatactcta cttcatcttt aatttggcgg tgcttgcgca ttccggctgt atctatcaaa
    21061 gtaaccggtt cattaaaata ttttattttt gtatcaatcg catcgcgggt cgtgccggga
    21121 acgtcggtta cgatagcctt ttccgagccg gctaagacat ttaaaatcga agatttgccg
    21181 acattcggca taccgacgat tgctaattgt aaatactctt tctcatcatc attagcggcg
    21241 atttttcgtg gaattctctg cagaatggca tccagtaaat cgccgatatg gcgtccattc
    21301 aaagccgaaa tcggaaaaac atcgcccaag cctaattcgg taaattccag actattaact
    21361 tccagtcgct cattgtcaca tttattggca acgattagac aatcccgctg ggagcgtcga
    21421 atcatctcgg caatttcacg atcgagggct gtcatcggtt cactggcatc tactacaaaa
    21481 ataaccagat tggcttcttc aatggctaat ctaacctgtt cacgaattgc tttcgttatt
    21541 tgattggtgc tttctgggat aaagccgccg gtgtcaatca gcaaaaattg tcgtcccgcc
    21601 caatttacag tttcgtaaac ccgatctcgc gtaataccgg cttccgaatg cacgatagat
    21661 ttacgcttct gggttaggcg attgaataaa gttgatttgc cgacgttcgg tcgtccaaca
    21721 attgctacaa tcggttcagc cattatcttc gtccattaat ttaacaaaat cgccatccca
    21781 aacctgtacc ggcttctgca aagtctcggc aatctggtag ggataaaaat catcgagtaa
    21841 aaccccatct tcattaataa tccgcggcgg taacacaatt aagtcatact catcaacatt
    21901 gattgcctga gcaagaatat cttgaccggt caataatcct gccaccgtta ccgatggtcc
    21961 aaagaaatta ttaattactg gtaaaatatc caccgagaag ttattgatct gattcaatcg
    22021 cgccgtgaca tatctactta aaaccggtgc cgccagcata cctgtaacga aaagcactct
    22081 tttcctctta ctaatagcgt gtggaaattt acgactttgc cgtttgaaat cgtcaagaaa
    22141 tgcccgcgtc gttccgactc cattttcaat ttggtaaaaa ctcccataaa atttgcaccc
    22201 cggtaaatct tgaccgacta acaaataaaa ttcatcggag agaaaaaccc aatgctcgcc
    22261 atcgcgattt tcgtaccggc gattccaagt tcgaatattt tcaagcaatt gagatgccag
    22321 atgcgagtct accgctggga gaagcggaag atggttgcga tgcgcagtca gaccgaccgg
    22381 cacgattgcc accgataata tcgaatcccg gaaaatatat agatccgcca acgttttttc
    22441 gagtaccgcc ccgtcattaa cacccggaat taggactatt tgagtatgca gatcaatccg
    22501 gttttgggct agaaaagtga gtttttgcat caaattatcc ggctttttca attgaaaaat
    22561 atggcgtcgc acattttcat cggtcgcatg aatcgaaacg tacaggggcg aaaggcgttg
    22621 caagacaatt cgattaagat cggcttcact taaattggtt agagtgatat aattcccgag
    22681 tagaaacgaa tagcggtaat cctcatcgca gaaataaatt gtcggtcgca ttccgggcgg
    22741 attttgcttg ataaagcaga aaatgcagtt atttccacag gctttcagac gcggcggaga
    22801 taattcaatc cccagtggtt catcggcatt tttagcgatc tggaatgttt tcttatgacc
    22861 atcccgaagg acaacaactt tacaattttc atcagattga tgaaaataca aatcgaggaa
    22921 atcgttgatc ggctggcgat tgatgctgac aatctggtcg ccccgacgga taccgttcat
    22981 ttcagccagg ctgttgggct ttactccgac aatttcaacc acgattccag caccatattt
    23041 tataattaat taaaaattga ttcatattaa taaaaactgt ccaaaattcg ctaatcccga
    23101 ccggaaaaac aaaattaatc cagaatgtcg aatttgacag aaaaccggtt tatcctgctt
    23161 tttaatacaa agactgggat ttgagcgggt gaggagaatc gaactcccgt gaccagcttg
    23221 ggaagctgga gttctaccac tgaactacac ccgcaatcca aaaaagcgtt gataatttaa
    23281 tagcactaaa taattttaaa aatgttttta attacttgat tggacaaaca ttacccagtc
    23341 tattttaaag tttttgtcaa agtaattagc atatattcac taacgcttat tacccaacaa
    23401 ttggtattat tctcgtcggt aaatacaaaa tcttgtattt ttacttgact acttggtatg
    23461 aacttactaa ctttcaccca gtttaacttt tgaaaattaa aatgggctca atcaattaac
    23521 tcaaaatagg agattttcgc gaatgtctga gtcgattacc atagatttta aaaatcctaa
    23581 aaacgaatcg cctgaaaaga atattccaga aaattctctg tcaattaatc ctgaaaatat
    23641 tcaagttaca cccgagaaaa aacctttatt ttccaaaaat gcgatggagg tattaaccaa
    23701 acgctacctt cagcacgata aaaatggcaa tgccgtcgaa acggtcgagg aattattccg
    23761 gagagtggct gaagcagtag ccgaagccga ggctaattat tctccgcgct ccagcattcc
    23821 ccgcacaacc gaaaaatttt accagatgat gattcgtaaa gacttcctcc ccaattcacc
    23881 aaccttgatg aatgccggtc gtgaactagg gcagctttcg gcttgctttg tcctaccgat
    23941 tgatgattcg atggaatcca tctttcagac tttaaaagac accgcactaa ttcataaaag
    24001 tggcggtgga accggctttt ccttcagccg gattcgcccc aaaaaaagca ttgtaaaaac
    24061 taccagtggg gttgccagcg gaccagtatc ctttatgaaa gtttacaacg ccgccacgga
    24121 agccgtcaaa caaggtggca cccggcgcgg cgctaatatg ggaattctac gcgttgatca
    24181 tcccgatatc ctcgaattcg tagattgcaa gaaaaatagc aatgagctaa ctaattttaa
    24241 tatttcggtc gccgtaaccg atgaatttat ggaaaaagtc cggcgacgcg aacagtatga
    24301 actggtcaat ccggttgaca atactatcgt cggaactctt aatgcggctg aagttttcaa
    24361 actgattgcc cagaatgcct ggtataatgg cgaccccggt ataattttta ttgaccgtat
    24421 taatcagatg aatcccactc cgaaattagg cgatattgaa gcaaccaatc cttgcggcga
    24481 acaaccgctc ctgcctaacg aatcctgcaa tctcggttcg atcaacttag ccaattttat
    24541 ttccgagaat aaaattgact ataagcgcct tggcgagact attgatttag ccgttcaatt
    24601 tctggacgat gtaattgaca tcaataaatt ccctctcaag aaaatcgaaa caatggtaaa
    24661 aaagacgcgc aagatcggtc taggtgttat gggatttgcc gacgttcttt tcagcttgaa
    24721 aataccttac gattcggatg aagcacttca gttagccgac gaattaatga aatttatcta
    24781 caaccgaacc aaggaagcct ctaccaaatt aggcgagagc cgtggtactt ttcctgcctg
    24841 gaacgatagc atttatttcc ccgatggtcc caaatatcgc aattcaacct gcatcacgat
    24901 tgcgccaacc ggtacaattt caatgattgc cgactgttcg agcggcatcg aaccgatttt
    24961 ttcattagca ttcactaaaa aagttatgga tggcaccgaa ttgctttatg ttaatcctta
    25021 tttcgaacag gcttgcaaag aagccggtat atacagtgca gaattgatgc atcgggtcat
    25081 cgccgaaggc tcaatccagc atattgcaga aattcctgag aaaataaggc gggttttcgt
    25141 taccgcacat gatatttcac ccgaatggca tgttcggatg caagccgctt ttcagaaatg
    25201 ggtagatgcg gcggtttcca agacctgcaa cttccccaaa tcggctacgg tgcaggatgt
    25261 cctggacgct tattggctgg cttatgaagc cggttgtaaa ggcatcacaa tttatcgaga
    25321 cggaagtcgg gacgaacagg ttctgtacgt cggcaaacag accgatcagg ctaaatcgga
    25381 gcaagccgtt cagcccccga taattgacca aaccctgcat ccgcgaattc gtccacccga
    25441 gactcatgga gtaaccaaac gcgttcggac gggtaatggc actttgtata tcacgatcaa
    25501 tgaagatgac tacgggctgt gtgaagtctt taccaatatc ggcaaagctg gtagtcaggc
    25561 gcaagtcgaa tccgaagcca ttagtcgttt gatttcgctt gccctgcgtt ctggaattga
    25621 cgcccgcgat gtggttaagc agttaaaggg catcggtggg ccttcgccga tctgggaaaa
    25681 cggcactcaa attctctcga ctccggacgc tattgccaaa gccctcgaat ggtatctgga
    25741 cgaacgcgaa ggcaagaaga aaaccactgg caaccaaccg agtttttcgc tactcgacgt
    25801 tgagagtaca acgacggtaa agaagcccga acctacttca caacacaccg cgccccataa
    25861 taaaaagtca attacgacct gtccggaatg tggttctacc gtttaccatg aaagtggctg
    25921 tgtgacctgc ccgaattgtg gcttttcgaa atgctaaact gcgtctgggt ttagaatcta
    25981 tactttgata catattttca tatacctcca agctataccg aaagcctaag gtcaccgagc
    26041 gattaaatat tttgcgtatc tgccatgtga atttgaaaac tcgaaaattc tatctgcctt
    26101 ggtcagtatt accgtttatc gtattttatt tttcaatagc ttaatccgtc aacctagtaa
    26161 tcttacagta tgtttctctc cttgcccctc attaattcaa tgcaaggaat atcagtataa
    26221 ttctttagct gataatggat tacaaactaa aattagcgat ccgatggcca gtgataactt
    26281 ccaagccaat atcgctggca cgttcaataa agccccgatg cgggcgatgg gacatgaaaa
    26341 taaatcctgt agctttcaat atcctaacag tttcatgaat agcctggagt tcaataaatg
    26401 actcgcgagt gatgggatag gagaaacatt gaaaaatata aaaattacac cctttcatca
    26461 tacagatatc aatcattgat tcacaatgca tggcttcagg gacactctcc cggtcaattc
    26521 gcacattcag gcggacatca tcgccctgac ggtgcaccct tagaaatacc aattcacgca
    26581 accacccacc attgaaaaga taattaccgc cgtcgccggc aatgtcaaca gttctctggt
    26641 tttcgcccgt tccaaagcgg aagcgcataa cattctgttc cgcatcataa ttaacaacga
    26701 aacgcaatcg ctgatcatca attttccaat gaaccgtctt tttattttcg cccatattat
    26761 tccattcgtc tcgatgcgaa tcaataaacg gcatgatccg atctagatta ttgccgataa
    26821 aaaatgaaag ggcgctacgt tttccgattt ccggatcgaa gcgtgtcccg gattccattt
    26881 taattccgtg cagagcaata tattcattta cagttagatt aaattgaaaa gtcttaacct
    26941 cgccggtttt gacatccacg attcgcgagt tgtcgggatc aatcaggatg acttccttac
    27001 cggcattttt aaaaacttca gccgccagaa cagcatgaag acgcgatccc ccactaatat
    27061 tcagaataac atcatgttca ggatagtctc tgagaacttt ctggcactgt tcgataaatt
    27121 cgggaacatc attcggattg atttcacggc tctcgaatgt cgagctaatt tgtccactta
    27181 aagcagtttc gattagcgaa acgacctttt tctgatttgg ggtgcggagc caaacaatag
    27241 cgtccggctt ttttaaaatt gcacttagat atccgattat cggtaaatcg tttaccaaac
    27301 aaacttccaa cagcgccata ttcatctccc atttctacta aaacgaatca tttattaaac
    27361 atttttagaa tttaataatt aatttgagtt tttcaataag tgattttaat aaaagaagat
    27421 aggctggcaa ggcgagtaat tatccgaatt accttaccct gaaaaattaa aaggcggtgg
    27481 aatttaattt aaagctaaat cattctccgc aaccagcgct tccgccataa tatctcttac
    27541 acaggtaggt ggtgcgggtg gcttacctgc ttgtcggtaa tgaaattctc tgccttcgta
    27601 attgcacata acgacttctt catctgtaat agacttgaca tattcgggta ggagagggat
    27661 ttttccgcct ccaccgggag cgtcaatcac aaaatgcgga accgccaaac cggaagtcca
    27721 gccgcgcaga gctttgataa tttctaaacc tttctcgact cgggtgcgga agtgttcggt
    27781 accatagacc atatcagcct gataaatata ataaggccgg acgcgcgctt ggagcaattt
    27841 ctgcatcagc aatttcatca cttcagggtc atcgtttacc cctttcaaga gcacggtctg
    27901 gtttccgaga ggaataccag catcagccag gcggctaaga gccttgaccg cttccggcgt
    27961 caactcatcc ggatggttga agtgaacgtt aatgtatagt ggatgatatt tcttcaggat
    28021 ccgaactaat ttcgtagtaa ttcgctgagg cagaaaacag ggtacgcggg tgccaatgcg
    28081 tagaatttga atatgcggta tttggcgtag gcttgataga atatgctcga ttttctggtc
    28141 actcagcatc agcggatcac cgcctgaaat gataacatct cgaatttcag gatgttctgc
    28201 aatgtattta attccttgtt gaataaattg cggactgatc ttctgtgcgt caccgacctt
    28261 gcgtttacgt gtacaaaaac ggcaatatga ggcacattcc ggagaaacaa gaaaaagaca
    28321 gcgatcggga tagcgatgga caatactggg taccggcgaa tctttttctt cgctgagcgg
    28381 atcgtattcc aagccgcaat cttccaattc agccttgtca ggtataacct gcctgtaaat
    28441 cggatcgcct ttctttttaa tcaatgaaaa ataatatgga ttgaccttga tccgaaagac
    28501 tttgtcaagc ccagccaact cgtcatttgg aaggtttaag gctttggtga tttgatccac
    28561 agtagtgtaa cttttttgga ccaatttgcg ccatttttcc atttcttatt tattctcctg
    28621 cttaaagtat tttacaaaaa ttactcggtc atcaccctgg cgataaaaat ctttaatttg
    28681 ggcggcaatt gcataaccat ttttcagata aaaattgcgg gttggtaaat atttctcctg
    28741 ggaggaagtt tcaattataa tcagatggcc atgctgggct tggacagcgg tttctacata
    28801 agtcaacaag tctgtgccga tgcctttgcc ctgaatctgg ggagccactg caatccagta
    28861 aagatcaaaa gtaccctcag tagcaggggt aggcccatag caaacataac cggcgagttt
    28921 ctggccattg atggctacaa atattaaata atcagtttgg ttaggttttg tcaaagcggt
    28981 gtcaatcaat tccatagcca ccctaatttc cgccgtagta aacatctggg tatcgcttaa
    29041 tattttcaga atctcggctc gatcggtgga ttttatctcg cgaattatgt tcatcttctt
    29101 cgcctgttca aagcctgttt tataagaaaa acaacaaatg tttcgtaact gataccggcg
    29161 gcatttaaag cccgaacaaa accagcagaa ggcgaaatat caggattggg attatattcc
    29221 agtacaaata aattaccggc ggcatccata cgaatatcaa ttcgtccata atctcgacca
    29281 cgcaaagcgt gataaaccgc taaactaagg ctggtcaatt tttcgttaag ttcggcacta
    29341 atcggagccg gacaaaccgg tggggtcatt tgataaacgg ggtgatcact gagccactta
    29401 gcttcgtagc tagtgatggg aaaatactct tcgctgacct gttcgaaatt aatttcagaa
    29461 ggtggcaaaa ccagtggatt ttcattgccg agaatactga cattgaattc gcggccggct
    29521 atatattttt caaccaaaat tggttgctta tacttagcca gtaactctcc gacgatcgac
    29581 tttaattcaa tcagatcatt gacgactgaa ttctgagtaa taccgatact accatcttcc
    29641 ctcgaaggct tagcaatcaa tggaaaatcc agactgcagg tttccggaat agattcgtag
    29701 acgtaaaaat caggcgtggg aattcctatg gctttaaaaa tatttttcga taacacttta
    29761 ttctgtgcca gaccaagcgt tagaggagaa ttaccggtat aaggcaaatc tagcatctcc
    29821 cagagtccgg cgacattcat ttcctggtcg gtgcgacttt gccaaccttc acagagattg
    29881 aaaattacat ccggctggaa ggagttaatt tttattaaat cctcatcaat gtttaaaatc
    29941 ggaagcattt cggctacgta accgcaatta ttcagagccg tataaacggc ggcagcttca
    30001 tctctaacgg cgatttccga aataatgtcc ggatcaaaat ttgaatctac cgattgcaaa
    30061 tcgttgaaga ccaccagaat tttgatcttc tgagattcgg atgacattag gacaagccgt
    30121 atctccgggc ggcgacctga acgacttggt ttaacattgc aggataggta taaccggcag
    30181 tgcgcgcggc tttgggaaaa caggaattat cttctggatt aggcaagatc cccggcaaag
    30241 gattgatctc taaaatatgg gcgaccccat tttcatccag acggacatcc aatcggcacc
    30301 agtctctaat atttaaaatc cggcaagttc tacgaacaag tagttcgatt tgattcttca
    30361 gattatcatc aatttgagcc gggcaggtga aaatatttag cggtttttct ggccgatccc
    30421 aaatccattt ggcttcatag gagtagatcg gttgagcacc ggtcggcaat tgtgaaaaat
    30481 ccataccgat aatcggcaga acttccattt cggggctatt gcctaggatt gcgaccgtaa
    30541 attccggccc agccaggtac ttttccatga ttaccggttc attattatat ttagcaaaac
    30601 aacttgcaat tacgtcatca gcttgttctt ttgagtaaac caccgaatta tcaaaaatgc
    30661 ctttgctact accttctgct aacggtttaa caatagccgg aaatttaaaa ttttccgcaa
    30721 aaccatcgtc cccgggatat atgacctgaa aggtagcatt cggaatgtga taatagctca
    30781 aaatttcttt acaacgtgct ttattcagac aaattgccag tgttaaagga tcggaaccgg
    30841 tatatggtaa acccagcctt tcgcagatta agggcacata ggattcgcgc caatcgccaa
    30901 atagcccttc cgtaacatta aaaacgatgt ccgggcggct ttgttctaaa ccgtgcgcga
    30961 cctcgtcatc accttcaaag ctgactacct gatgattatt taattggaga gcatctacga
    31021 ttgcctcaat cgtttgaggg tcatcaccct cggcaaaaag gtcgggagga atgtcttcgt
    31081 cgtccggatc gacggcaaag cgcttacgat actcctttct caaaccttct tttgtgctat
    31141 acgttaatgc aattttcatt ttttatggcg tgaaatttac atattatttt tgaaatgcaa
    31201 gtaaaatatt tttataaatt gaaatttctt taatattcta aaaagcccaa tcctttgggg
    31261 aatgctattt taggattaca aattgttgta taggaaaata tctccaacct aacgcgggtg
    31321 ggcaatctca aaggctttac ggacaacagg atagtattgc gggtctttca aaaaggtctc
    31381 gtaagagaaa aatactatat tttgaaattg attattcaaa gttaatatcg ttttagtcat
    31441 agcctcgtat tgactctgat tatagacact aataccgacc cagacattat ttttatttat
    31501 attagggtca attttcctca ggattttttg gaattgctta gtatctttgg tatagttcat
    31561 caagaccacg aaatccagcc agtcttgcct taaccaatat tgccagtctt gaaagaaatt
    31621 tttacaggca tcgtccggat cgggcttgac ggccgcactt aattgtagag acggtcgaat
    31681 ttctcgtaaa ttcttgcggg cattatgaac gaaatcagaa atggcatctc gccgaaagtc
    31741 ttcccatttg agcattaaag aatcaatttc cgccagttct aatcctttat aaagtgattt
    31801 gtcggagagg gttaagagga gaggatcaat tccatatttc tcacgaaata atatgcggcc
    31861 aataggattg taatcgtagc aattttttgc ataacgaacg taatcaagat gaatgccatc
    31921 gaggtcataa ttggtggcta attcctgaat aatatcaatg aggtatttac gtacttctgg
    31981 taagaggggc gataaataga ttccttcggt acccaaggtg ttgaaatcag tggcacggcg
    32041 ttggatatct tttataccat tggcatctac cgtacaccac tcgggatgtt gatacaaaag
    32101 gtgattttca tttttcggtt tctgatcaga tgtccatagc aaaaatacat taacccaggc
    32161 atggactctc aatccataac tatgggcttg gttgagggta tattccagtg gatcccagtt
    32221 attgttgttt agtaactcac tatagactac aatttcggag tgataaaatg catcaccgcg
    32281 accacgaact tgaacaaaca gatcttttat atcatttaga acggcgaagt taacgaagcc
    32341 atctattttc tctttagaat tgagctggtc gcgaattacc cataagccga ggttggtctg
    32401 ggctcgagcg gtgaccgtta gactataaat tagaagggca gtcatcgcga ctgccctgat
    32461 ttttaaagat aattgatttt tcaaaaacaa tccaacaagt ttatttactc agtctccgat
    32521 gcgggttcgg ctttttcgcc tgatatattt tctggtggga tatcttcttt tacttctgct
    32581 tgggctgatt gtttttcttc ttttttggcc tgagtttttt tggtaggctt ggtcttagct
    32641 ggagtttgtt ctttttctac ttctgttgat ttttcctctt tcttcccctg aactgattta
    32701 gtcgcctctg aaccggcctt cttctttttt tcttccggtt gtttataatc tacaaattcg
    32761 attaaagaaa cagccgcatt atcattatcg cgatagccta gtttaataat tcgagtataa
    32821 cccccggtgc gatttgcata ctgcggagca atgtcttcga ataatttatt tactaaatcg
    32881 cgatcctgaa taaatcggaa agcaaggcga cgggaatgga cagtatctct cttgccataa
    32941 gtaatcaagc gttcaactac gcgacgtaat tcttttgctt ttgcgtcagt agtttttatt
    33001 ctaccattta tcagcattgc cttcgttaga ctttgtatta gcgccttgcg ctggtcagtt
    33061 gatcggccta atttagctac ctttttctgg tggcgcatat cagtggtact ccttttgggt
    33121 tattcttctt tctcgacgag gtatttatca acatccatgc cgaattccaa gcccatgctt
    33181 ttcaatttgg tcattaactc atttagagat tttcgtccga aatttttgaa ctttagcata
    33241 tcggcttctt catgggaaac cagatcggca atggtacgga tgttgttggc ttttaagcaa
    33301 ttgtacgatc taacggataa ctccatttca tccacgctct ttttgagcag attcctgatt
    33361 ttaaggacat cttcgctggg ttcttcacgt tgaaattcga tcggtttagc ttcgacgcgt
    33421 gaaaaaaggc taaagaaatc gcgtagaatt ttagaggcta gatcgaggga ttcatcggga
    33481 gtaatgctat catcagtcgt aatttccagg gttagtttct cgagaatttc acgggcggta
    33541 ccgggcacat tttcgacttt ataggataca ttaagaatgg gtgaaaaaat cgaatcaatg
    33601 aaaatcgttc caatcggata atcgggcagc ttattcttct ccgctggtac ccaacctgtg
    33661 cctcgggcga tcctaatctc aatattgaaa tcggcatcct cattcatcgt acagatatgt
    33721 aaatccggat tcaagatctt aaactgggta atattttcgc caatcattcc ggccgtaaat
    33781 tcaccagcgc ctttaaaatg gagagtgact ttatctggct tgggatcgaa tagtttgagg
    33841 cgaacttgtt tcagattaag aattatttcc gcaacatctt cacgtactcc aggaatagtg
    33901 gaaaattcat gcaaaacgcc atcaattttg atcgaacaga ttgccgcacc cggtatcgaa
    33961 gtcaagagaa cccgtcgcag ggcattaccg atggtgatgc caaaaccccg ttccagcggc
    34021 tgtagataat aaatgccatg agtgtcatta gaattttcgt ctttaacgac tctggcgatt
    34081 atttgatcgt aaacagtcat tatttccctc cttatttaga ataaagttcg acaaccaatt
    34141 gttcgttgac atcaacgttc atctgatctc tttccgggac ttggatgaaa attccctgca
    34201 ttttggcttt gtcaagaatc aaccagggta gttcgtgctc accacggatt cgtttcatcg
    34261 aatcatgaat tacctgcaat ttcttgcttt tttctctaac ctgaatgaca tcaccagctt
    34321 tgagttgata ggaagggaca tcaacgacac ggttgtttac ggtaaaatgg cggtggctga
    34381 ctagctcgcg tgccatccgc cgggatgaag caaagccaag ccgatagacg acattatcca
    34441 gtcttctttc cagaatcttc atcaggttag taccggtgac gcccttctgg cggttggcta
    34501 attcgaaata ttggcggaac tgtttttcta gaacgccata aattctctta attttctgtt
    34561 tttcacgtaa ttggattcca taatttgaca aacgtggatg agcagtctga ctgccatgtt
    34621 gaccggggat ataacttttc gtttcaaaag agcatttggt gctgttacag cgttcgcctt
    34681 tcagaaaaag ttttttaccc tcccggcgac atagtttgca tactggacct gtatatcttg
    34741 ccattaaata ttctccattt tagaaattat actcttctcc gctttggagg ccggcaaccg
    34801 ttgtgcggta tcggggtaac gtccttaata gctgaaattt gtagcccggc taaattcagc
    34861 gctcggatag cggcttctct gcccgcgcct gggcctttga ctcgtacttc gacgcgctgt
    34921 aatccaagat taatggcttc ttgagctgct tttgtggcgg cttgctgaga agcgtagggc
    34981 gtgttcttgc gcgagccttt gaaaccggct gttccagctg aagcccagga aattacatta
    35041 ccatagttat cggttagtga tattatcgta ttgttgaagg ttgctttgat ggtagcaatt
    35101 ccttccgatt caacttttgt gcgtttcttc ttttttacgg tagaacgttt gggtggcaaa
    35161 tcaaatacct cctattttat ccttttttcc cgacttttac tttcttacgg ctaactgtgc
    35221 gccgtggtcc tttgcgcgtg cgagcattgg ttttagtccg ttgaccgcga accggcaaac
    35281 ctcgacgatg gcgcaagccg cgataacaac caatatccat taggcgtttg atgttcatct
    35341 gaacttctgt gcgcaaagta ccttcaactt tatattcatt gctaatcaca ttgcggatat
    35401 cgcgcacctg ttcttcggtt agcgctttta cccggatttc aggatcaact cccgctttct
    35461 gcaggatatc catagcatta gcccggccaa ttccatagat gtatgccaag gctattttca
    35521 ctttcttttc gttcggtaaa tctacaccgg caattcgagc caaattttcc tcctaattgc
    35581 ttttatccct gtcgctgttt atgttttgga ttggtacaaa taactcgcac tactcctttg
    35641 cgacggataa ttttacattt gttacaaatt ttcttaactg atgatcttac tttcattttc
    35701 tatatccaat cttcatttat agcgataagt tatgcgaccc ttggtcaaat catagggtga
    35761 tagctccatg gtaaccttat cacccggtaa aatcttgata aagtgcatcc gcattttacc
    35821 ggaaacgtgc gccagaactt catgtccatt ctcgagcact acccgaaagg aagcgttcgg
    35881 caaattttcc ttaattattc cgtcaacttt tatcaatttt tcttttacca cttttttttc
    35941 ctttatcctc tttgtgataa aatttgcggt ccatccggac caatagcaat cgtatgttcg
    36001 taatgggctg aaggtgaatg atcctctgtg attaccgtcc aaccatcttt caacaaacgg
    36061 acttcataac ttcccatcgt aatcatcggt tcgattgcca gacacatacc cggtttcaaa
    36121 actggtccgc gacccggttc accgtaattg ggaatctctg gcggctcatg cagttcgcga
    36181 cccaccccgt gcccaaccag ttcacgcaca actccataac cgtagctctc gacgtgggtc
    36241 tggatggcat gtccgatgtc cgataaatga tttccgggtc gagcctgagc tatgccttga
    36301 tagagtgaat cctccgtgac gcgcattaat tgtaatcggt catcagagac tgttcccacg
    36361 gcaaaggtca ttgccgcatc gccatagtag ccatttttct tgacaccgac gtcgatgctg
    36421 ataatttcgc cctctttgag aactcggttg gaaggaattc cgtggacaac ttgctcatca
    36481 atgctggtgc atatattgca gggaaatccg ttatagccct tgaaagccga atgcccgcct
    36541 tggctcttaa taaattctgc cgccagatga tcaagataga gcgtcgttat accaggcttt
    36601 atctctcgac ttaacaaatc gagagtttca gagacaatcc gacaactttc acgaatcttc
    36661 tcaatttcct cggctgtacg aatatgaacc attatcgcta attatccgcg gcggcccttt
    36721 aattttccgc ttttcaaaaa accatcataa tgtctcatca aaagatgaga ttctatctgc
    36781 tgtaatgtat ccaaagctac accgacgacg atcaggagac ttgttccccc aaagaaagaa
    36841 gccagatcat aattaatgtc cataatcttc atcagtatat agggaaaaac agcaatgaat
    36901 gctaaaaaga tcgagccggg taacgtgact ttggttagaa tattatcaat aaactccgcc
    36961 gtctttttac caggtctaat tcccggaata aaaccgccat acttttttag attatctgca
    37021 acttctaccg gattaaaggc aattgctgta taaaaataag taaaaaagac gattaaaact
    37081 ccataaatca gccaatatac aattgattgc acactaaata ctcgcatcat tccctggacg
    37141 aaatcactgt tcggaaagaa agtagcaata gtgctcggaa tgaaaacaat cgactgcgca
    37201 aaaatgatcg gcattacacc ggcggtattg acccgtagag gaatgtgagt cgcctgcccg
    37261 ccgtaaactt tccggccaac gactcttttg gcatattgca ctggaatacg acgtgtgcct
    37321 tgggtcaata gcaccacgaa tccaacaatt actatcatta atacgattaa aattatttcc
    37381 gtaaataatc cgcggacacc ttctcgaatc agagtaattt cgctgacaaa cacgtttggt
    37441 aaacgtgcca ggattccgat cattatgatc atagatgtac cgttaccaat acccctttct
    37501 gtcatacgtt cgccaagcca catcagtaaa accgtaccgg ttgttattgt cagcattgtc
    37561 attaaaacga aaccaactcc cggtcggggt acaactggta gcatttgatt gcccacccgg
    37621 gctgacatct ggggactcat cagaaattgt gctactccaa atgattgcag ggcggcaata
    37681 ataaccgtac cataacgcga catctgggtc aatttttttc gcccttcttc accctctttt
    37741 tgtaattttt gaaaatatgg gattactgca ccgagcaatt gtaagataat tgaagccgta
    37801 atataaggca ttatacccag cgcgaaaagc gtcgcttgag caaatgcccc tccggagaac
    37861 atatcataca gtccaaagag tgtattcgcg aaactactca ttgccgcacc cagtacttca
    37921 gtatcaatac ctggaatcgg tatatgggct ccaattctga aaactatcag aagggcaagg
    37981 gtataaccga tgcgttttct caattcggga atcttaaata tactctggaa ttgctggatc
    38041 ataaaagttc tacctttcct ccggatgccg ttattttctc tttcgcgcgg gtactgaagg
    38101 catgcgcaac gacggtaata gcgcctgaga gttcacctcg tcctaaaatt tttaccggtt
    38161 tttttaggct tttaatgagg cctttttgtt gcagaatttc cggattcact tcggttattt
    38221 gtaaacgagc aatatgatct aaattgacta cttgaaattc ttctttgaat aaatttttaa
    38281 aacctctttt gggtaaacgg cgatagattg gcatctgtcc accctcaaca tagacgggtt
    38341 ttttattgct accccgtgca ccggttcctt tattaccgcg accggctgta ccgccgtgtc
    38401 cggaaccagg tccacgcccg aggcgctttt tattttttag tgaaccgtaa gccggtttaa
    38461 gtttgtttaa gcccatatcc tattccttaa tctcttctac tctaagtaaa tgcggaatgg
    38521 agttgaccat accgcggatt gctggattgt ctggtaaaag aaccgtctga ttcattttag
    38581 taattcctaa ggcaagcaag gtgcgtttgg ttttttcaca atagccgatt ccgcttttta
    38641 cctgagtaat tttcagcatt gcagttttat ctttttttgt catgaaccaa atatctcctg
    38701 aagcgttttt tcgcgtctat gagccaccat gtaggggtct tttaatttcg agagggcaag
    38761 cattgtcgct ttgactacat tgtgcgaatt attagagccg agcgatttgg ataaaacatc
    38821 gcgcacaccg aggctttcca taatagcccg aatcgcacca ccggcaataa cgccggtacc
    38881 cggagtagcc ggacgcagaa tgactttggc ggcgccgtat ttagcctgga cttcgtgggg
    38941 tatggtaccg ttgatgaccg ggatttttac caaatttttc ttggcgtttt ctttgccctt
    39001 agccaccgct gaactcacct cgagggcttt gcccagacca atcccgacgt gccctttgcc
    39061 atccccgatt gctaccaggg cgttgaagcg aaacctccgc ccaccggtaa caacctttgc
    39121 gactcgatta atttttatga ctttttcttc cagtaattcc agttgagttg aatctatagt
    39181 ttttctcaag ttaccattcc tccgctatta gaattctaat ccggcttgac gtgccgcttc
    39241 tgctaaagct tttacgcgac catgatattg atacccgctc cggtcaaaaa ctactttagt
    39301 aatacctttt gccacagctt tttcggctaa ggttttaccc accagagcac ttcggtcgga
    39361 ctttttagta ttgcttttga tctgctcgcg gacttcaggc gtaaggtctg aaacggcgag
    39421 aagtgattta tgaaggttat catcaacaac ctgggcataa atatgattca ggcttcggaa
    39481 aaccgtcaga cgcggccgct ccgacgtacc ttgtattttg tgtcggttac gtttctggcg
    39541 gcgctgtcga gcgaatgctt ttatattctt gactttattc atgtataaaa cctctatttc
    39601 acaccaactt ttttaccggc ttttctgcgg atatgttcat tctggtaacg aattccttta
    39661 cccttataag gttcacacgg tttgatagat cgaatttttg ccgcaatttg tccgactttt
    39721 tgtttatcaa taccttttac gacaatagta agggctttgg gaacctcaaa gcttacccct
    39781 tcgggaggcg taactaagat tgcatgggaa taaccaacat taatttgaac ggatttaccc
    39841 ttcatctcag ccgaatagcc gacaccttcg atttccagag tcttgctgaa tccttgcaat
    39901 acaccctgga tcatattagc gatcattacc cgcgtcaagc cgtgtaaagc tttatgattg
    39961 cgactatcgc tcgggcgggt aacaattatc tgactatcgt ttatctgaac tttcatatcg
    40021 gacggaaaat cccactttag ttcacctttg ggacctttga ctgtaatgct acaatcttgg
    40081 attgtaacat tgacttcttt aggaatttcg attggttttt taccgattcg tgacactcta
    40141 tactccttga atcacctttt taccagacat agcaaagaac ttcaccaccc acattatttt
    40201 ttcgggcgac tttatcggtc aggacgccca ttggagttga taaaatagct attcccattc
    40261 caccgactac ccgagggata ttatccgaag ccgcatagac acgccgacca ggtttgctga
    40321 ttctttgcaa accttcgatc atcggttcac cagttttaga atagcgtaag tagatacgaa
    40381 tgagtccctg tttgccgtct ttgattaaaa taaagtcctt gataaaattt tcgtgcttta
    40441 aaataagcga tattctcttc tttaaattgg aggcaggaat atcaacccag cgatggttcg
    40501 cggcttgggc attacgaata cgagttaaat aatctgcaat tggatcggtt accgccatat
    40561 catatactcc tgtattacca gcttgccttt gtgatacccg gaatttcacc gcgcagggct
    40621 aattcccgaa aacaaagccg gcaaagacca aatttacgat aataaccgcg cggacgcccg
    40681 caaatcgaac aacgattgac tctccgcgta ctgaatttgg gatttcgcct ggctttggca
    40741 ataagtgatt ttttagccat cttagtttat ctccattgcg gtcttgactt caccgccggt
    40801 tgttgattct ttgattgctc ttttcttgaa cggcaacccg agaagggata gcaattcata
    40861 cgcttccgta tcggttttag ccgtggtagt aaaagtaatg ttcattcccc gaatatgatc
    40921 aactttatca taatctattt ccgtgaaaat aatctgttcc ttgagaccta tcgaatagtt
    40981 accgcgacca tcgaaagatt tatcagaaag tcctgaaaag tcacgtactc gcggtaaagc
    41041 tgtcgaaata agccgatcca taaattcata cattcgaaaa cgacgcaggg taacggcaca
    41101 accgaccgga tctccagttt taattttaaa attggaaatg gcgcgttttg ctttagtcac
    41161 catcggttgc tgtccagtaa taatacgaat gtcttcaaca gctttcttca agctggctgg
    41221 atcttccttg gttttgccca ctcccatatt tatgcttatt ttgacaagtt tgggaacctg
    41281 cataatattt ttatagccga acttctttac catagctgga actatttcgg tctcgtatgc
    41341 ggtcattaag cggggtttat atgcagactt ttttgccact tatagctcct aattatatag
    41401 catcaataat ttcaccagtt tcgcgtgcta cacggacttt tttcccatct ttcagagtcc
    41461 gatagccgat ttttgttgcc tgaccagcgt gtactaattt gacattagat atatggatcg
    41521 gcgcttcttt ttcaataata ccaccttgag gattttgctg gttggggcga gtatgccttt
    41581 tgataaaatt tactccttcc acgattactc gttctttatc cgggaaaact ttcaaaacct
    41641 ttccgatttt gcctttggat tcccctgtta taaccattac tgtatcattt ttacgtatgt
    41701 gaagcatttt tatattcctt tataatacct cgggcgccat agagacaatt ttcatataat
    41761 ttttctcgcg cagttctctg gcaactggcc caaagatacg ggttcctctc ggctcaaggt
    41821 tattgtcaat cagaacagcg gcattgtcat caaagcggat atatgagcca tctttgcggc
    41881 gaacttcctt ggtcgtgcgc acaactacgg ctttggattt ttcgcctttt ttaaccatac
    41941 caccgggcgt agcctgtttt atggttacaa cgatggtatc gccgactgtt ccgtatttac
    42001 gatgggaacc gcccattacc cggatgacca ttacttcttt tgctcccgta ttgtcggcta
    42061 cttttaatct tgattcttgc tgtatcatag aaatatcctt tgtggtttgt cctagcgttc
    42121 aattatttcc agcagacgcc atctcttcaa ccggcttaag ggacgcgatt cgacaatttt
    42181 aactttatcg ccttcgtgac atttattctc ggcatcatgg acataaaatt tggaagtccg
    42241 tcgaatatat ttgccataag tgctgtgctt tacagttcgc tcgacggata cgacagcggt
    42301 tttatcttgc ttatgactga tgactgttcc aattaaaatt tttcttctgt cgcttgaaat
    42361 cattcttgat tgtcctgtga tttatttatt cggtttatct tttgatctga ttcccagttc
    42421 gtactcatgt aaaacggttc gaatttgagc aatttctttc ttgacgtgcc tgatcttcag
    42481 tggattttct aattgctgaa gagctttttg aaagcgtagg ttttccaagg cggttaaatt
    42541 gtcgcgtaat ttagtttcca gttcattcac cgttaattca tcaagagtac tttttttcac
    42601 tttatgctcc taattctctt ctagctacga ctttggtttt aatgggcaat ttatgggtag
    42661 cagtcttgaa tatgtcttta gcttcttgtt cgctggctac ttcaatttcg aataaaatac
    42721 gtcccggttt taccacggcg acccagaact caggtgtccc tttgcctttt cccatgcggg
    42781 tttcagctgg ttttttagtg ataggtttat ccgggaagat ccgaatccac attttaccat
    42841 gtttgcgaat ttggcgcgaa attgctactc gaacagcttc gatctgccga ctatcaatcc
    42901 agccgggttc catagctttt aaaccataat gaccgaaact gacggcattc ccacgggttg
    42961 cgattcccgt catccggcct ctttgctgtt ttctccattt tatctttttc ggtgccagca
    43021 tgattaatta ttcctttttt aacttttctt gaatctctcg ccgttacaaa tccagacttt
    43081 gattccgatg caaccgtaag ttgtatgcgc tgttacagcg gcaaaatcaa tatcggctcg
    43141 caaggtatgt aaaggaaccc gtccttcgcg catagtttct gtacgggcaa tttcggcgcc
    43201 gcctaaccgg ccactgcatt gaacccggat tccttccgca cccatacgca tggttgattg
    43261 aatggctttt tttacagcac ggcgataaga tacttttttc actatttggg cggctatatt
    43321 ctgaccaacg agtgaggcat ccagttcggg gcgcttgatt tcattaacat tgatctggac
    43381 atcggttttg gtgactttct gtaattcgag tcggagtaat tcaacttctt cgcccttttt
    43441 accaataaca atgccgggtc gcgaggtgtt aatggtaatt gtgatttttt taggagtccg
    43501 atagatatcc actcttgaaa tgccggcatt aggaagacgt ttcaaaacat aacgccttaa
    43561 tttgatatcc tctgttaatt tttcagtgaa attcctttca tcaaaccagt aactgctcca
    43621 ctccttgttg aatcccagac gaaaacctat tggatgcgtt ttttgaccca agtcacctcc
    43681 gattattgta ctttactttt ttgtttattt tctttttccg ccacaactac agtaatatga
    43741 cagagccgac gcctgatgat actggctcga cccagtgatc cagcccgaaa gcgtttagca
    43801 atcgagccgc catctacgta gatagtcttt agatagagat cttccggatt taatttcgaa
    43861 ccgccctcac tattcaagag gttggacact gccgaccgaa cgaccttttc tacgttctct
    43921 gaggcttttt tactattaaa gtgtaggagg tttaaagccg tttcaacgtc tttcccccgt
    43981 actaaatcag caaccagacg gactttccgc gctgattgcc ttacatatct atgaactgct
    44041 ttggcttcca taactcgatt taggctccct tcttttccgc accggaatgc ccgcggaaga
    44101 ttctggtatg ggcaaattcg cccagcttat gacccaccat attttcggta acgaagaccg
    44161 ggatgaattt attaccgttg tgtacggcga aagtgtgacc gacaaactcg ggagagatcg
    44221 tagtgcgacg tgcccaggtt ttaatcactc gttttttccc ggagctattc atttgctcga
    44281 ttttcttcat caatttaaca tcaatgtatg gacctttttt cagcgaacga ctcatccaat
    44341 attctcccta attatttagt tcgtctcttg ataataaact tattgctggg attacgtttt
    44401 ttacgtgttc gatatccttt tgataactgc ccccaaggtg aacatggatg ccttccgccc
    44461 gagctcttac cttcgccacc acccatcgga tgatcaatcg gattcatagc cacaccccgt
    44521 acgtgcggac ggcgacccat ccaacgactg cgtcctgctt ttccaatact gatattttcg
    44581 tggtctttgt tacttacgac gccgatcgtg gcatagcatt cttcattgat gagccgcatt
    44641 tcacccgaag gcatttttaa agtcacatat ttgccttctt tagccataat ttgagcggct
    44701 gtaccggctc cgcgagcgat ttgccctcct tttttcggac gcatttcgat attgtgtaca
    44761 aaagtacctg cgggaatatt ttttaacggc agacaattac cgaccttaat gggggcttct
    44821 tcacccgaga gtacaacgct accggtaacg agtccatctg gtgcaattat atagcgtttc
    44881 tcaccgtctt tatagaatag aagcgcaatg cgtgctgatc gatttggatc atactctata
    44941 gctttgacag tcgcttcgat tccatatttg ttacgcttga agtcaatatt ccggtaagca
    45001 cgcttttgac cgccaccccg atggcgcgaa gttacccgac cgtaattatt gcggccggca
    45061 gattgcgtaa tcggttcgag cagacttttt tcgggtctct ttttggtaat atcttcaaaa
    45121 gtatcgaccg tcttaaagcg ctgtccgggt gtaatcggtt tgatagtttt aatacccatg
    45181 atactatacc gccgtttctc cttgataaaa atctattttt tcaccctcct tgagggttac
    45241 aatggctttt ttccagtttg accgacgccc agtagtgcgt ataactcgac cgccactccg
    45301 gactgtcatc agttttgttt tacccataat gttctgagta gcgacttttt ctactttgac
    45361 cccaaactta tcttcaacag cctttttgat ttcgattttg ttggcagagg gatgaacttt
    45421 aaagccatat ttattttggc tttcctgtaa ttcgttaatt ttttcggtta gaattggttt
    45481 gataataatt gacttagaag acatatttag ttcctactat cgcccaacaa ggcattattt
    45541 aacttttcga agccgctttt atcgattaaa aggacttcgc agtccagcag atcgtaggtc
    45601 gaagcttttt cagcttcgag gatcaataca ttgtaaaaat tgcggctggc taacaataga
    45661 ttagtattta aattagcggt tagaatagtc acttttttat ccaagaggtc aaattttttc
    45721 agtaattcgc ggatatttct ggattttggc tcgtcgaagg ccaattcctc gatgattttt
    45781 agggcacctt cgcggtagcg gtaagccaga gcactttggc gcgccagtga tttgactttt
    45841 ttgttaattt tcatcttata gggatgcggt ttgggaccaa aagcccgccc accgccgacc
    45901 cagaccgatg accgacggga gccagagcga gcccgaccag tacctttttg tttccatggc
    45961 ttcttcccac caccccgtac ttcaccacgg gttttggtag aatgagtacc ctgacgacta
    46021 ttagtcattt cggttttaac ggctaaataa atagcatggt cgtgcggttc tatatcaaag
    46081 gctggggtct cgattactaa tttctggcca gaatctgaac catctaattt ataaacagaa
    46141 aattcgatac tcaccgttcc tatctcctaa tagttagaat actgtttttt gaaccaggta
    46201 cggcgccttt gatataaagc aagttgcttt cggggtcaat tttaacaacc gttagattat
    46261 tgaccgtgca acgcttgccg cccaaacgtc cagccatacg cactcctttg ataacatgcg
    46321 atggataggc ggaagcgcct atggaaccgg gagcacgtaa acgatcactc tgtccgtggg
    46381 tttttgggcc accgccaaaa ttgtggcgct tgacaacacc ttgaaaacct ttacccttgg
    46441 aaatgccggt aattttaacc ttatctccag gattgaagag cgcaacagtt actgtttcac
    46501 ctacctgaca aaatttagga tcgtaatcac gaatttctac cagatacttc aggggagtaa
    46561 tattattctt cttgaaaacg cccagattag gcttagccat tagacgttct ttggcttcca
    46621 taaatcccag ttgaaccgag gcataaccgt ctttttcatt cgttttcacc tgaaccactt
    46681 tgcaaggccc agccgcaata actgtcaccg ggatcaattc accattctcc ttgaatatct
    46741 ggctcatgcc aatttttttt ccaattattc cgatcataat gtatccattt ataccttaat
    46801 ttcgatgtcc acaccagctg gtaaatccag tttcatcaga gcatcaacag ttttggaagt
    46861 ggagttcaga atatcaatca gacgcttatg aatcttggtc tgaaattgtt ctctggattt
    46921 tttatccaca tgtggagaac gtaaaaccgt atataccgtg cgttgggttg gtaatgggat
    46981 cggtcccgag accatcgctc cggtggattt agctgttgct acaatctttc ttgtactttt
    47041 gtcaagtaaa ttatgatcgt aggcttttag actaattctg attctttgcc caggcacagt
    47101 tctactcctt attcaatgat ttcggtaacg acgccagcac caactgtccg tccaccttca
    47161 cggatcgcaa accgcaactc cttctccatc gcgatctcac taatcaactc aacactcatc
    47221 acgacattat cacccggcat taccatatct acgccttccg gcaaggtcac tactcccgtc
    47281 acatccgtcg tccgaaaata aaattgcggc cgatatccat tgaaaaacgg ggtgtgacgt
    47341 ccaccctctt ccttcttcaa tacatatacc tgacccttga acttccggtg cggcgttata
    47401 ctcttcggcg ccgctattac cataccccgc tgtaaccaat ccttctctat tccgcgcaac
    47461 agtaaaccta cattatctcc agcctctccc tcatccaata tcttccgaaa catctctact
    47521 cccgttacca ccgtcttacg tgtcggacct aatcctacaa tctctacctc atcgcctact
    47581 ctgaccttac cgcgatctat ccgacctgtt cctaccgtcc cacgtcccgt gatcgaaaat
    47641 acatcctcta ccggcatcaa aaatggcttg tcgatcgcgc gtaccggatc cggtatgtaa
    47701 ttatctaccg cttccatcaa ctcaaatatg cacttcgtct tctccggatc atctggatga
    47761 ttcaatgcct ctaacgcact acctcggata atcggaacct catcacccgg aaaatgatac
    47821 ttattcaata aatctcgcaa ctccatctca actaaatcca ataactccgg atcatctact
    47881 aaatctacct tgttcataaa tactactatc gaggggacat tcacctgata ggctaataat
    47941 acatgctctc gcgtctgcgg catcggacca tcaaaagcac taactaccaa tatcgctcca
    48001 tccatctgtg ccgctcccgt gatcatattc ttgatgtaat ccgcatgacc aggacagtca
    48061 acatgtgcat aatggcgctt cttcgtctca tattcaacat gtgaggtgtt gatcgttatc
    48121 ccacgctccc gctcctcagg ggcattatct atactatcaa acgtccgctc atccgctaac
    48181 ccctgtcggt gcaataccat cgttatcgcc gccgtcaacg tcgtcttacc atgatctaca
    48241 tgaccaatcg taccaatgtt gaggtgcggc ttcgtccgct taaattgttg ctttgccata
    48301 tttcagctcc ttaatttttt aaaaactcag ggacaaaacc cctggctttt tcaataattt
    48361 ctctttgaac tcttttatca cagtaatcga attgggaaaa tttcatagtg aaaaccgctc
    48421 gcccttgtga aattgacctc aggtcagtgg cgtagccaaa catctcgcgc aagggggctt
    48481 cagcgtcaat tacattggca tcgcctcttt tattcacatt gattatatta gctcgccgtg
    48541 tattcagatc actaagtaca tcaccgagat attcatccgt tactaccact tccagcgcca
    48601 taatcggctc aagcaaagcc ggttcggctt tgtgtaaagc attctcaaac gccatagccg
    48661 cggctacttc aaaagccagt tcggtggatt cttcttcacg atattccgca ccagtgataa
    48721 taacacgtac tttttgtaat gggtatccgg ctagtactcc gctttttaca tgattgcgta
    48781 tacctttttc aatagccggg acaaattctt ttggtaaagt acccggaacg attcggttct
    48841 cgattcgcac ttcattgctt gcggaatccg gttcaattct caatcttaca actgcaaatt
    48901 ggcttttatt cccggtttga cgaacgaatc ggccttctcc ttctgcgaca ctcacgaccg
    48961 tttcgcgata tgaaacctgc ggcttgccga tgtgggcttt gacattaaac tcccgaatca
    49021 ggcgttccac cagaatttcg agatgtaatt ctcccatacc ggcaatgaga atctggccag
    49081 tttcttcatc ggtagaaatc tcaaaggttg ggtcttcgtc tgccaattta ttcaatgcat
    49141 taatcaaatt ttcctcatca gccttgcttt tcggttcgat agaaattttg ataacgggtt
    49201 ctggaaattg cattgattcg agaataatag ggtttttagg atcacaaaga gtatgcccgg
    49261 tataactgta ttttaggccg acaattgccc cgatttcacc cgcttccaac atctcacagt
    49321 cttcacgttt gttggcatgc ataagaagta aacgactgat gcgttctttt ttctgaatgg
    49381 tcgagttaaa aaccatatca cccttgctta tgacgccact ataaattcgg ataaaactta
    49441 atctgccaac atggggatcg gacataattt taaaaatcag cgcgcttagc ggctcatccg
    49501 gacgcggata gcgtttgatt ggcgtgcctt ttaaattgac tccctcaata ggaggcatat
    49561 caaaaggtga tggtaaataa ttaacaattg cgtccagcaa aggttgaatt cctttatttc
    49621 ggaaagccga accgcatagt accggaacga ttttattctc gagagtaccc tttcgaagac
    49681 tattctgtag ttcctgcgga gtaatatctt cgttatttaa atatttggca aaaactacgt
    49741 cgtcaaaatt ggcgacttct tcgatcaatt tctggcggta ttgagtagca atatttttta
    49801 aatctttagg aatctcaata taatagtatt gacttccgag agaattatcg tcatataaca
    49861 tagcctgctg agtaatcaaa tctattattc cggtaaacag ttcaccagac cccaatggca
    49921 aggtgactgc taccggctgg gctttcagcc gtttctcaat catatccaga acgttaaaga
    49981 aatcggcgcc tgtgcgatcc attttattga caaaggcaat tcgtggaatg tgatatttat
    50041 cagcttgccg ccagacagtt tcactttgag gttcgacacc accaaccgca caaaagatcg
    50101 ctaccgcgcc gtctaaaact ctcagagagc gttccacttc agccgtaaaa tctacatgtc
    50161 cgggtgtatc tataatattt atgcgatgtt gattccagta gcaggttgtg gcggcagatg
    50221 taattgtaat tccgcgttca cgttcctgct ccatccagtc catagtggct gaaccttcgt
    50281 ccacttcgcc gattttatga acttttccgg tgtaaaacag aatgcgctca gttgtagtag
    50341 tcttaccggc atcaatgtga gccatgatgc cgatatttct gactttgctg agattttctg
    50401 atttcactct actttatcct caatatatca tctgaaatgg gcaaaagcct tgttggcttc
    50461 agccattttg tgtgtgtctt cgcgtttttt gatagcatta ccttcaccgt tagcggcggc
    50521 gagtatttcc gcggctaact tttctgccat tgattttccc ggccgagcct tagcataggt
    50581 aattagccag cgggaagcta gagcaaagcg tctttcaggc cttacttcga taggcacttg
    50641 ataagtagct ccaccgattc gccgtgattt gacctccata attggtgaaa cattattaat
    50701 agctttttta aacacatcaa ggccattacc tttatgcttg gcttcgattt gagcaatggc
    50761 gtcataaaat gaagcctcgg cgacggattt tttacctttt cttaaaaggt aattaataaa
    50821 gcgagctacc ataatgtcgc cgtatttggg atcgggtaag acttctcggg atataacttt
    50881 ttttcgacgt gacatacaat gttctcccta ttaggacgga cgttttgttc cgtatctgga
    50941 gcgactgttc ttgcgatcat tgacaccact ggaatccagg gtaccgcgga taatatgata
    51001 acgaacaccc ggtaaatcct taacacgacc gccttcaatt aacaccatcg agtgttcctg
    51061 gagattatgg ccctcacctg gaatataagc cgttacttcg attccattgg ttaaacgtac
    51121 acgcgctact tttcgcaaag ccgaatttgg cttcttggga gtagttgtat aaacgcgggt
    51181 acaaacgcct cgtttctgag gggaaccgcg taaggcgggt gctttttctt tcttactgac
    51241 tacttcgcga cccttacgaa ttagctggtt aatagtcggc atattttctc cataataaaa
    51301 gcctgtaaaa atacgacact taaaaatttt aaacaataaa ttttaacaac cagatgttta
    51361 tttttggaga gcggcgagct cattttcttt ctctttcttt tgttcttcta attttttcca
    51421 ctcttcgatt tcttcttctg gttcaatcac gcttatatct ttgtatttac gtaaaccggt
    51481 tccggctgga atcaagcgtc ccattatgac attttctttc aaaccgcgta ggtaatcggt
    51541 ttttccagct acggcggcat cagtcaaaac tcgggttgtt tcctggaaag aagccgctga
    51601 aatgaatgac tcagtattaa gtgaggcacg agtgataccg agtaacattt cggtaaattg
    51661 ggctggacga gccgggcgag ccttagctgg attcttgcct tcttccttaa gtaatcggtt
    51721 gaatttggcg aattctttct tggaaacgac atcgccttca tcccaatcgg aatcaccggc
    51781 attgacgatt accacgtaat ccttgatacg attgttttct tcaataagtt catggcgatt
    51841 aacgcgatct ttttccagca aattcgtatc gccgggatcc tcgatgcgaa ccttctgcat
    51901 catttgacgc acaatgactt cgatatgttt atcgttgatt cggacaccct gtaagcgata
    51961 gacttcctga atttcgttaa cgagatattc ttgaactttg cgtggatctt gcactgccag
    52021 aatatcctgg ggcgcaactg ggccttcgca taatcgctca ccagcccgaa cctcatcccc
    52081 ttgattgacc agaacgtaac gaccgtaggg aatcttatat ttcttcgttg ttccgtctat
    52141 accggtaacg ataatttcgc gaacaccttt ctcggtttta ccgaaagtga ctttaccatc
    52201 aatttcactg ataacagctg gatcttttgg ccgccgggct tcaaacagtt cagctacccg
    52261 tggcagaccg ccggtaatat cgctcgtttt accgatctct ttcggttttt tggctaaaat
    52321 gtctccttta acgacttcat caccatctcg aacgactaac atagcacgca ccggtagtat
    52381 atacccggga taaaccactt cttttccatt ttcctgagca acgatatcaa tacgtggttg
    52441 tagcgagcgg tctttggact caatcacggt catagtctgg tgaccggatt catcatattc
    52501 gatattataa gtcacgtttt cgattaaatc tacaaaacgt actttgccgg aatagagggc
    52561 tagaatctga tccatataaa catcccagcg gaaaagggta tgaccggctt taaccaattg
    52621 gccttcattg acaaaaacat gcgcaccgta gggaatgtta taagtagaag taatccggtt
    52681 gttatcatca accaggttca agacggaatt atgggaaaga acgactgaat agtctgaatc
    52741 tttgtatttc tcggcaacat aattgctgga aaattgtaca tagcctttcg ttttggcaac
    52801 agtttccgat tcttcgacaa tgcgcgccgc cgtaccaccg taatggaaag tacgcaaggt
    52861 caactgtgtc cccggttcgc cgatggactg ggcggccata atcccaaccg cttcaccgac
    52921 tttgattaat ttaccgttcg caagattgcg tccataacac ttggcacata ctccccgaag
    52981 agattcacag gtcagaacag aacggatacg aactctttcg ataccagccg actgaatttt
    53041 cagggcggtt ttttccgtaa tttcctcacc tacttcaacc aaaatctcgt cttcactttc
    53101 cggattataa atatcttcct gagctactcg gccaataata cgatcggcca gtgattctat
    53161 aatattttct tcctttttca aggcggtgac ttcgataccg ttaatcgtct ggcaatcttc
    53221 ctcggaaatc acaacatcat gagctacatc tactaatcgg cgagtcaggt aaccggcgtc
    53281 agccgtcttc aaagccgtgt ctgccagtcc cttgcgggct ccgtgagtag aaatgaaata
    53341 ttcgtgaact gtcaaacctt ctttgaaatt ggaagtaatc ggcgtttcaa taatttcacc
    53401 gacttgaccg gtcatagatt tttggggttt tgccattaga ccgcgcattc cagccagctg
    53461 cttaatctgt tcaccgcttc cgcgcgcgcc agaatcggcc atcatataga ccggattgaa
    53521 accctgacga tcagtcgcca tatgttcaag cataacttcc gaaaggcggt tggtagtgtg
    53581 cgtccagtta tcaattactt tgttataacg ctcaccttca gtcaatattc cctttctaaa
    53641 tttgcgctga atatcttcta ctctttcaaa tgattcctgt agaatcttct ccttttcctc
    53701 aggaattaac acatctgaga tagagatcga gactccggaa cgagtggcat atttaaagcc
    53761 gatatttttc aaattatcga ggaacttaac tgtttcatag ttgccagctt cctggaatac
    53821 gataccaacg attttttcaa gacttctctt gctgattaac tcgttgtaat accccattcc
    53881 gggtggcaaa agactattga ataaagcgcg accgatagtc gtttcgataa ttttaccatc
    53941 aatcctaatt ttaactttgg catgcaaatc tacttcgccg atttcatagg cgtaaattgc
    54001 ttcttccggc gaagaaaaaa ccagaccggc gccccgcgca ccttctttct gacgggtaat
    54061 gtaataaaca cccaaaacca tgtcttgcga aggaatagca atcggatgac catgcgctgg
    54121 atgcaaaatg ttatgactgg ataacgccag caaacgacat tcgacctggg cttcaaaggt
    54181 aagtggtata tggacagcca tctggtcgcc atcgaagtcg gcattgaatg ccgcacaaac
    54241 taacggatga atttgaatgg ctttgccctc aattaatttc ggctgaaaag cctgaattcc
    54301 taaacgatgt agagtcggag cacggttcag aagaaccggg tagtccttga ccacatattc
    54361 cagaacgcga taaacttcgg catccttgcg ttccaccata gtcttagcgg ctttgggcgt
    54421 cttggctagc cgacggcgaa ggagagcgtg aattatatgg ggcttaaaga gttcgattgc
    54481 catatcttta ggcaaaccac attcattgag acgtaaatct ggtccgacga cgataaccga
    54541 acgtccactg taatccacac gtttccccag taaattctga cggaaacgcc cttccttacc
    54601 tcttagcata tcgctcagac ttttgagcgg acgacgcgtg ccactgcgaa cttctgttcg
    54661 acggcgacta ttgtcaaata atgaatcaac tgcttcctga agcattcgct tttcattgcg
    54721 caaaatgaca tcaggagcct tgatttcgat taattgtttt aaacgattat tgcgaataat
    54781 aacccgccga taaagatcat tcaggtcgct cgccgcgaat cgcccacctt ccagtggaac
    54841 cagcggacgc aaatccggcg gaataaccgg taaaacggtt accaccatcc attctggttt
    54901 atttatcggc ttgccttctt gattcaagaa agccatcacg actttcaggc gttttagaat
    54961 ctcggccttc tgcgtcggtg acttttttat ttttaattct tctttcagac tatgaaccag
    55021 gccttctata tctacttttt ggagcagttc ccgaatagct tcgccccctg ttttagcaat
    55081 aaaatagttc ttagcttctt cttcatcaaa aagctcggtc ttcggaccat aagcctgcat
    55141 attttcaaaa tattcttctt cggtaataac cgttttatat tttaggtcgg atttgccctg
    55201 atttaaaact acatatctct cgtaataaac aatctcttcc aaattcttag tcggaatatc
    55261 caatagataa tataacttgc taggaattga cttgagaaac cagatatgga caaccggtac
    55321 agccagggta atatgtccca ttctttcgcg acgaactttt ttgcgagcga cttctacgcc
    55381 gcaacgatcg caaataatgc ctttataccg gatacgttta tattttccgc aatggcattc
    55441 ccagtctttc actgggccaa aaatcttttc acaaaacaac ccatctttct ccggcttata
    55501 agaacgataa ttaatcgtct ctggctttag gacttcaccg tgagacctgg ttaaaatggt
    55561 ttcaggtgaa gaaagatgga tcgtaatact ttcaaattcg ctacgaaatg tagatggttt
    55621 tgtataattc atactgataa ttatacctcc gtaatgggat taattaatat ccatttcaat
    55681 gcacaagcct tgtaattctt tcaataaaac attaaaagat tccggcacac cgaaatcgga
    55741 gaaattttcg cctttgacaa tggcattata gacttttgaa cgaccgtcaa cgtcatcgga
    55801 tttgactgtc aagatttcct gcaaagtgta agccgcacca taggcttcca gcgcccagac
    55861 ttccatttca ccaaatctct gaccgccaaa ttgagccttg ccacccagcg gttgctgagt
    55921 aactaatgaa tacggaccgg tggaacgagc atgcattttg tcttcgacta agtgtgataa
    55981 tttcatcata tagatgtagc cgaccgtcac cggattatca aactgctcac cgatttgacc
    56041 atctataaga ttaaccttac cgatttccgg taatccggct ttgcgcaatt cttcctgaac
    56101 ttcctgctcg cttgcgccat tgaacacggg agtttcataa taaacaccaa gttcgcgccc
    56161 cgcccaacct aacatagttt catacaattg ccccaggttc attcgggaag gtacacccat
    56221 cggatttagg ataatatcta ctggtgtacc gtcttcgagg aaaggcatgt cctctttagg
    56281 aacgattatt gagacaacac ctttattacc atgacgacca gccattttat cgccaacttg
    56341 aactttccgt ttgctggcaa tatagacttt ggctaattgt aagacacccg gctgaaggtc
    56401 atcgccaact ttaatcttat aaatggtttg ttctaattct tcatcaatcc gctgactaag
    56461 aataaaataa cttttaacga ttttctcaac tttttcgttg atttccggat catttgtcca
    56521 gggcgtttcc agactgagta gatcgaaatc caactgatcc agaatttcat cattaaattg
    56581 gatgcctgac ttgatagcaa ctttgccatt ggttagattc ttaatcgtgg cacttgtagc
    56641 acccttcaga atatttttca atagaagatc gcgtcttcct ttaaggtaag catgcttttg
    56701 aacactgcgt ttggtttcct cgtcaatctg acgtttttct tccatccgac tgactttact
    56761 tttgcgggta aaaagtttgg tatcaacggc aacgcctctg atccccggat cagcccgttt
    56821 ggaggcatct ttgacatcac tggctttttc tccgaaaatt gcccgcagta atttttcttc
    56881 aggagtcgga tcggtctcac ctttgggagt aactttacca attagtatgt cattaggttc
    56941 gacttccgcg ccaacccgaa tgattccgtt ggcatctaaa ttcttagtag cctcttcact
    57001 gacattcgga atttcgcggg ttagttcttc gtttccacgt ttggtatccc gaacttccag
    57061 ctcgaattcc ttgatatgaa tagaagtaaa tacatcatca tagaccaagc gttcgtttag
    57121 aataatggca tcttcaaaat tatagccgcg ccagggcata aaagccacca acacattacg
    57181 accaagcgcc aactcgccat cattggtagc acaaccatcg gcaattgcat caccggcttt
    57241 tactcgatca ccttctttaa tcaaaggctt ctgattaatg caggtatcct gattagtacg
    57301 gagaaactta gccaattcaa tacgaacctt accttgattt tcatctaaat acaattcgtt
    57361 ctcagtgtcg gtgcgaatta cgatgtgatc ggcggtaact tcctctacaa tgccgtttac
    57421 cggggcgagt acaaccgacc gcgaatcgcg ggctactacc ccttccagac cggtaccgac
    57481 aatgggacgc tctggtttca acaatggtac tgattgtctc tgcatgtttg aacccattaa
    57541 agcccggttg gcatcatcgt gttcaagaaa tggaattaat gcggcggaga ccgagacaat
    57601 ctgctccggt gaaacatcca tatatttaac ttcgtcgggt gttacaattg ggaattcacc
    57661 cctaaccctt gccctcaccc tagaattaat aaaatgccct tgttcatcca gcggttcatt
    57721 agcctgagca attatctcgc ggtcttcatc atccgctgac aggtactcaa ctttatcagt
    57781 taccgtagcg attccgttga gtttttcaac acggcgatat ggagtttcga taaaaccgag
    57841 atcattgaca taggcatagg tagcgagaga agcaatcaga ccgatatttg gaccttctgg
    57901 agtctcaatc ggacataatc gtccgtagtg agtatagtgc acatcacgca cctcaaatcc
    57961 agcacgttcg cgggataagc cacccggtcc taaggatgag actcggcgct tatgagtgat
    58021 ttcagccaga ggattggtct gatccataaa ttgggacaat tgactagtcc cgaaaaacga
    58081 atttattacc gaagttataa tgcgtgaatt aattaaatct tgaggggtga gattttccga
    58141 ttcgcgtaga ttcattctct ctctgattgt gcgcgccatt ctggtaaatg ccagattaaa
    58201 ctggttggtg agctgttcac caacgtttcg cacccgacga ttcccgaggt gatcaatatc
    58261 atctgggccg agattgccct ttttcatatc aacgatacgc ttcataatct cgacgatatc
    58321 atcgcgagtt agaacacatt cttccagtgg aactttcaga ttaaattttt tattaatccg
    58381 ataacggcca acctgtccca aatcatactt cttgggactg aaaaataagc gctcaacaaa
    58441 ctttttagca atatcgagat tcggtggctc gccagttctt agactgcgat agagaaaata
    58501 gagcgcagat tcctcatcat tggtttgagt atctttgcgg aaggtattta aaaaaatatt
    58561 gcgttcgatt tcatcatcgg tcgtgaatac taatagggaa gtaattccat gttgtttgca
    58621 ttttttgaga aaatctttat caacgacgat ctcttctttg atttcagaaa taacttcgcc
    58681 ggtctcggga tcaatgacgt tttccgccag catttttccc agaagctctt tggaatctcc
    58741 atcaagggaa aagcgtttgc cgattccgaa taagttgact atgtcttcat tggtcggata
    58801 gccaaaggca cgcaataaaa tggtcgccgg aaatttacgg cggcgatcta tgatagcact
    58861 caggatatca cgagtatcaa ttacaaaatc aatccatgaa ccccggaagg gaataactcg
    58921 tgccgaataa attttcatgc cattggtatg ttcggtttca tcaaagaaaa cacctggtga
    58981 acgttgcaat tgactgacaa tagcgcgttc agcaccatta atgataaagg tacctttttc
    59041 tgtcataaag ggaacgtttc caaagaaaat atcctgctcg ataccagcct gatactcgcc
    59101 tttggaaata ccttcttcac tgatatgaag caccagacga acttttaggg gtacagtata
    59161 gctgacacct cgttcgagac actctttgat agtgtaacgc ggttcaccaa ttgtgtaata
    59221 tttgtactca agaagataat tgccgtgggt atcttcgact ggaaaaacgt ttctgaacgc
    59281 tgattccagt ccaatatttt tgcgttccag cggcatcgta tctttttgta aaaattcatc
    59341 gaaagatttt aactgcactt ccagcaggtt aggcatcttt tgcacaattt taactttggc
    59401 aaaagatttc cgttctttct gtaaagggag agtattactc aacggtatcc ctcctgatta
    59461 atttgatttt ccatataata caatgaaaat tccttattgg caaaacaaga attctcataa
    59521 atgctgaaga ttgaaactga gtgtagttta cttgatttcg actttggctc cggcttcttc
    59581 gagttgtttt ttaagttcag ccgcttcctc tttggaaatg ccctctttga ccgtactggg
    59641 agcgccatca accaaatctt tggcttcttt caatcccaaa ttggtagccg cccgcacgac
    59701 tttaattacg ttaatcttat taggaccaac ctctttaaga ataacattga attcagtttg
    59761 ttcttcaact gcttcggctt cagccgttgc cccaggctga gctaccgtag ccgcaactgc
    59821 gacgggcgcg gcggctttca cgtcgaattt tttctcgatg tccttaatga gttcagaaat
    59881 ttccagcata tttgcttttt ccagatactc cagaacgtct gctctagtta tgttcgccat
    59941 ttctaaaaaa cctcctttta gtgcgatgct tatgattgtt ttttttgctc atttaatgaa
    60001 tttagagtgc cgactaactg atttaacgta gcacgtagaa ccatagtcag ctggctcatc
    60061 ggcgcattta gagtagcgag tagagaagcc agtagttcgc ggcgggaagg caaattaatg
    60121 actttctcaa cttgatcggc tccaaacagc actccatcat agatacaacc ggtaataacc
    60181 agcttatcca gtttattttt cttaataaaa gtgttgatca ctttcccggg tatagcggga
    60241 tcagtatagg aaattgctaa cgcagtttgg ccatctaata agggctcgat cgaatctaat
    60301 cctgaatttt tagcggccat cttggtcaaa gtctttttaa ctactttata ttcgacatca
    60361 gccgcatata attccttacg taagtcggtg atttctttga catttagacc tttatagttg
    60421 gtgaaataga taccacgggc tttttgaaat ttctcactga tcgtttccag actctgaata
    60481 tttttctgat ttggcatttt tgctcctgag attacgctat aattgaggat ttatctaccc
    60541 gtactccggg acccatcgtc gtggttagag tcactttttg aatgtatgct cctttaacag
    60601 aaggtgggcg taaacgcata agtgttgaga ttaaagtctg tacattctct tcgagatgct
    60661 cgattggaaa tgacttttta ccaacgatag tatgaacgat tccggtctta tctactcgga
    60721 tttctactcg accggctttg atttctttta tagccgcagc gacatcatta gtcacagtgc
    60781 cacttttggg attcggcatc aaaccttttg gtccgagtat ttttcctaat ttaccaactt
    60841 gtcccattac atccggagtg gcaatgataa catctacgtc agtccagccg ttttgaattt
    60901 tttcgagata ttcttccaga ccagcataat ctgctccggc ttgaagggct tcatctactt
    60961 tactcttagt caatactaaa actcgaactt tgcggccggt gccgtgcgga taggttactg
    61021 tgcccctgat attttgatcg gcatgacgcg gatcaactcc caaattaaca tgggcttcga
    61081 cggtttcatc gaatttggct ccgttgcaat tttttagtaa ctcgagtgct tcccgcagag
    61141 ggtattcctt cttccggtca acttttttac tgttctgttc aactcgcttt gaaatgttca
    61201 taacgttgaa tcctcttact catttattga aatgcccata ctccgggcag tacctttaat
    61261 catttccgcg gctttttcaa ccgtgtgggc atttaaatca ggcattttta ttttagcaat
    61321 ttccatgact tggtccattg ttaccgatcc gattttactt ttattaggtt cacctgagcc
    61381 tttttctact ttggcggctt tcaatagcaa aacggatgcc ggcggtgtct tggttataaa
    61441 agtaaatgaa cgatctgaat aaaccgtaat aactacggga atgatataac cagcttgatc
    61501 ctgagtgcga gcattaaacg ccttacagaa ttcaacgata ttgacactat gctgacccaa
    61561 tgccgggcct accggaggtg acggattggc tttgccagct tcgatttgca atttaatagt
    61621 ggctaagact tttttcgcca tattacacct atttctccaa ttctacttgt aaaaagtcca
    61681 actcgactgg tgttggacgt ccaaaaatac ttactgaaac ttttaattta cgtttatctt
    61741 cattgacttc ctgaactact ccgcggaaat ctataaaagg gccatcaatg actttaaccg
    61801 ggtcacccat tttaaacgga atcgctaaca cctcccggcc gtctttcttc tcaacttcgc
    61861 ccatcactcg tttaacttca tcttctttca aaggctgagg tttaccggcg gctcccacaa
    61921 agttcattac tccgggaata ttctcaatca gatattgcga ctctttagtg atatccatat
    61981 tgaccaaaat atagccggga aagaatactt tgttgcgaac gcgtttctta ccctcacgca
    62041 tttccataat attttctgaa ggcaccagta catctttgat aatatctttc atcccagcat
    62101 gctcggcttc aatcagaata gcttctctga cttttttttc cttaccggaa tatgttctta
    62161 atacatacca ttccatatcg gacctgctaa aaaattacat tcataatttt tgatagaatc
    62221 agatcaacaa cgaaaagaaa taccgataaa atcaatgaga aaatcagaac aacggcggtt
    62281 gaacttttaa gctccgacca ggtcggccag gagacttttt tcatctcgaa gacaacccct
    62341 tcaaaaaatt cgcgaatgcg tttgatcatg tctttgtcct ccgtttgggc gatcggtaag
    62401 gtctattagc aatattgcag gtgaggcagg aattgaaccc gcaacccccg gttttggaga
    62461 ccggtgctct gccagttgag ctactcacct ttagttgcat attaacgggt ctctttatga
    62521 actgtatgtt ttttgcacca acgacagtat tttttatatt cgacccggtt aggctgtagc
    62581 ttcttattct tatcagaaga ataatttcga cgtttacaaa ccgtgcattc cagagtaatt
    62641 aattcacgca tattattccc tgaaatttat tcaatgattt cggtaacgac gccagcacca
    62701 actgtccgtc caccttcacg gatcgcaaac cgcaactcct tctccatcgc gatctcacta
    62761 atcaactcaa cactcatcac gacattatca cccggcatta ccatatctac gccttccggc
    62821 aaggtcacta ctcccgtcac atccgtcgtc cgaaaataaa attgcggccg atatccattg
    62881 aaaaacgggg tgtgacgtcc accctcttcc ttcttcaata catatacctg acccttgaac
    62941 ttccggtgcg gcgttatact cttcggcgcc gctattacca taccccgctg taaccaatcc
    63001 ttctctattc cgcgcaacag taaacctaca ttatctccag cctctccctc atccaatatc
    63061 ttccgaaaca tctctactcc cgttaccacc gtcttacgtg tcggacctaa tcctacaatc
    63121 tctacctcat cgcctactct gaccttaccg cgatctatcc gacctgttcc taccgtccca
    63181 cgtcccgtga tcgaaaatac atcctctacc ggcatcaaaa atggcttgtc gatcgcgcgt
    63241 accggatccg gtatgtaatt atctaccgct tccatcaact caaatatgca cttcgtcttc
    63301 tccggatcat ctggatgatt caatgcctct aacgcactac ctcggataat cggaacctca
    63361 tcacccggaa aatgatactt attcaataaa tctcgcaact ccatctcaac taaatccaat
    63421 aactccggat catctactaa atctaccttg ttcataaata ctactatcga ggggacattc
    63481 acctgatagg ctaataatac atgctctcgc gtctgcggca tcggaccatc aaaagcacta
    63541 actaccaata tcgctccatc catctgtgcc gctcccgtga tcatattctt gatgtaatcc
    63601 gcatgaccag gacagtcaac atgtgcataa tggcgcttct tcgtctcata ttcaacatgt
    63661 gaggtgttga tcgttatccc acgctcccgc tcctcagggg cattatctat actatcaaac
    63721 gtccgctcat ccgctaaccc ctgtcggtgc aataccatcg ttatcgccgc cgtcaacgtc
    63781 gtcttaccat gatctacatg accaatcgta ccaatgttga ggtgcggctt cgtccgctta
    63841 aattgttgct ttgccattgg tgctcctttt ttttattttt cctttacaaa aattctcaat
    63901 ttgatttgac acttacccaa aaaccgttta aggtatgagc ccacgaccag agttgaactg
    63961 gtgaccccat ccttaccaag gatgtgctct acctactgag ctacgtgggc attattccga
    64021 taaatcaatt acttttcttt taatcgcaag ttgagcgggt gaagggaatc ggaccctcgt
    64081 agccagcttg gaaggctgga gctttaccac taagctacac ccgcagaagc tgtgggccgg
    64141 gatgggttcg aaccaccgta ggcattacgc cggcagattt acagtctgct gccattaacc
    64201 actcggccac cgacccgctt ttatttatgt tttggaaaga taatttgtat tcgcaaaatg
    64261 ctattttaaa tgtttgactt agcgaaagta attactctct cccaaattta ctctgcaatt
    64321 caggctttgg tatccgtcaa atctccattt caaatagcat catcatcaaa tcctagagcc
    64381 accagtagga tttgaaccca cgacccgctg attacaaatc agctgctcta ccaactgagc
    64441 tatggtggca ggtcgtctaa caaatttcaa aaaagcctcg aaatttaatc aatttattaa
    64501 ttaaatcaat aagtttttat ttcaatagaa tctcaatttt taatttatga caattacttc
    64561 caccaattta aaaatccaat ttttgtaagt aggttactcc ctgtaaagta aagttctcga
    64621 gggtgaataa tctctactat actcaaggtc ttccgagcca atttggaatt aatagtttta
    64681 gattagactc taccgcatag caagcaaaac atttgtgaac ggtctaaccg gaaaaaccac
    64741 tactaaaata agattataaa acctatcacc actccattta tagttgatag acataaaata
    64801 ttttgggaaa tcttgttatt aacgctttaa caatctagtt cgtcttcgta cttttcaatt
    64861 ccaattctat aatccgcaaa atcttctcta ctttactaaa ttccgatatt cgctgagtag
    64921 ccaatagcta catattttta taagtttatt tcaataataa acactttcca acctgttgaa
    64981 aattgtcagc tcgaaattta tataagtaaa cacctgtact ataatttcct gcattccatt
    65041 gaactgaata ataaccagcc ggttgaaact gatttactat tttatctacc aatctaccgt
    65101 taatatcaaa tatggatagt tcaacaaaag attcgctcgg tagctgatag gaaaatttag
    65161 taaccgggtt aaacggattg ggatagttct gtacgagaat agcttcttga ggagtgttaa
    65221 ttccactttt ttctactgaa acaagtggtg gagtcggaat cgtaagagtc cggtaccaaa
    65281 tctcatcgtg tcctgttgtg gtatcaatcc aggcaaccgc catcgttgtt tcgtcaataa
    65341 tgcatacatc gaaaccaata ccagaccatc cagcagcagt attttcaact ggattgatac
    65401 gaacagaatc tgaccatgaa atagcatcgg tactcgtttt aacatacaga ttaggatcag
    65461 aaggatattg agttagcacg atcgttccac cgggagtaag tgcaatctga gaattacttg
    65521 cggggagcgt attaacaata gaccaatgtg ccccaaaatc tgttgaacga tagagccgcg
    65581 tgccatccat atccatcggt ggttcatcct ccccgggctg ttcagtttca tcccagtgct
    65641 gtacaaattg ccaaaactga ccttttgcat agatagtacc attcaataat ccaactattt
    65701 gaagatcata aaagtcactc cagaatcctt cgagactgtc aatcttggtg ggctcggaaa
    65761 aagtacttcc ttcgtctgaa gatgatataa aaaagagaga cgaatcttgc gcataaatca
    65821 agaagagatg atcagtgccc tcaactggta tcacagtgaa cgagtaatct tttattaatg
    65881 agagagagtc tccgagtact ttcctctttt gagaaaaaag ggagccgtaa gatttcgaga
    65941 aataaatgct attatcatcg atctgatttc tccagaataa ataagctcca tttgctccaa
    66001 cttgcagatc ctggtttgta taagtcatca taattgaatg tccaattgaa accgtatcta
    66061 atattatcgg gattgtccat tcgggacttc cgattgtttg ggaaacatat cgtacatcat
    66121 attgtccata ctcagaattc tggttaaaat cagtgtacac aatatacacc tgtccattag
    66181 catgggctat atcaacattg gttggtcccc aagcattcat atctggttta accggatttt
    66241 cgatgggtga tgtaggacct gttagtggga aactgtagaa attaaaattg gttgcaaacg
    66301 taatcattgc ttggtcgcct actacatcca atttaggttt attaacgcta tatccgttca
    66361 ttgcggtaac ctgataggct tgcccaaaga gcaacgaaga cgctagcatt aaaagtgtta
    66421 atagacaaac tttgataatt ttcataacaa aataactcct tttttaaagt ttaatttcac
    66481 ggcttattag agctcatttg ccctatatta gttacatcat cccggcgaat ctttggggat
    66541 ttttattcca agcgagtatt ttgacgctgg gctgtgttca tcaaaacctc ttaaaatata
    66601 ttgggttaaa agttcactca taagtcaaaa aactgctctt aaaagtataa aagtgtagtt
    66661 tattaaatcg atctaatgta gaatatttta ataaaacttc gacttatttt aaagcaattt
    66721 ttgggaaata ttggtgaatt ttctgtattt aatttcggaa aaccacccaa tctccaagaa
    66781 accagctcgt tatgtcagca atcagaaatt cacacccgat aacaattact actcacttga
    66841 catttgtttt ggggcacatg ttaccaaatt tgttcaagcg ctcacccatt cctacttttt
    66901 tttatctgaa caaatcattt gaatttctca cataattggt ttaatctttt gcgtttattt
    66961 ttaggtgtga ttaagtgagt attctcgtaa ctggaagtgc cggatttatc ggttttcatt
    67021 tagctcgtca gctcctgcag gagggcatag aggtcgtcgg atatgataat gtcaatgatt
    67081 attacgaccc gtcgttgaag gaagcccgtc tgcgaatttt gaatgacttt gacaaattta
    67141 aattttatcg tgccgatctg tgcgatttcg ccacccttca aaaagtcttt agcgatcaaa
    67201 cgatcgaaaa agtggtccat cttgccgccc aagctggggt acgctactcg ctgattaacc
    67261 ctttcgctta ccagaaatcg aatttggaag gttttttgaa catcatcgaa ttatcaaagc
    67321 gcgctaaggt caagaatttc atttatgctt ccagttccag cgtttacggc aataaccgcc
    67381 acctgccatt ctccgtccaa gacaatgttg atcgcccaat ttcgctctac gccgccacta
    67441 aaaaatccaa tgaattaatc gcacatgttt atgctcatct ttatcaactg ccttgcacgg
    67501 ggttgcgttt cttcacggtt tacggacctt atgggcgacc ggatatggcg ctgtttattt
    67561 tcactaaaaa tattcttgag ggtaaaccga ttgacgtcta taattttggt cagatgaagc
    67621 gcgattttac ttatattgat gatat
//
LOCUS       DAOSMP010000007        67170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_442_length_67170_cov_17.326022, whole genome shotgun sequence.
ACCESSION   DAOSMP010000007 DAOSMP010000000
VERSION     DAOSMP010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 67170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 67170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67170
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_442_length_67170_cov_17.326022"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1234)
                     /gene="wecB"
                     /locus_tag="PKX09_02090"
     CDS             complement(170..1234)
                     /gene="wecB"
                     /locus_tag="PKX09_02090"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOU16645.1"
                     /translation="MSTIATIVGARPQFIKCAPVSREIRRYFDEILIHTGQHYDYNMS
                     QAFFAELNIPEPDYNLEIGSGNHGEQTGKMLIELEKTLLKIQPDLVLIYGDTNSTMAG
                     ALAAAKLQIPIAHVEAGLRSFNRQMPEEINRIVADTLSQYLFAPTQTAVRNLCHEGFD
                     DQIHLVGDVMYDAFLYNSQIAEKSDILERLGINSQPYYLVTIHRPQNTDDPNTLKALI
                     STLEHLNELIIFPIHPRTRNLMRQFNITADSPNLRFVEPVGYLEMLKLEKQSKLIITD
                     SGGVQKEAYFARVPCLVLRPETEWVELVEYGWAKLIGNDFQRLPEETQKFAVFNRENT
                     ILFGDGNASLKIAEIIKDDF"
     assembly_gap    1419..1518
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1823..2056)
                     /locus_tag="PKX09_02095"
     CDS             complement(1823..2056)
                     /locus_tag="PKX09_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16646.1"
                     /translation="MSKQTTLKYLNCLMGIAFLGIIVSLFLLKTGIVTSDAIVEVHEI
                     FGGLLILGVIGHLILNWKWVKQTYLRRKKPNQN"
     gene            complement(2082..3425)
                     /locus_tag="PKX09_02100"
     CDS             complement(2082..3425)
                     /locus_tag="PKX09_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16647.1"
                     /translation="MYDQFVRDFYFPLTQHIKGEKIAQYLKILRYNEHLPLEKLQKIQ
                     LAKLYHILDVAYQNVPYYQAIFNQLGIKPENISSPQDFAHLPLLNKKDIRKNLSRLTN
                     PNYSGKIYSGKTSGSTGIPLKFYITNEYSSWDWASRWRARNWFGVQIGDPEVAIWGRP
                     LHSTIRKVIDALKGRFRNTLLISGFEYSEETLEKLSRQIWHFNPRYFYGYSNSIYRLA
                     LYYKENNLPLPGRLKAIFVTAETLFPQERKIVESVFHVPVSNEYGCSEIGGFAYECPA
                     GNWHVSIENVFLEFIENENGVREIVGTSLTNEYMPFIRYRIGDIGEWINGKCPCGCNL
                     PIMKLHIGKATDIVVMKNGQTFSSEIFDYLNLALLDLNRQPFDQYQIIQTQLSTFTIR
                     YVKSHNYSDSDLDLFQKLLRKTVHDDNIQIEFQPVTAIKPDQTGKMRYFISELKP"
     gene            complement(3425..4597)
                     /locus_tag="PKX09_02105"
     CDS             complement(3425..4597)
                     /locus_tag="PKX09_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU16648.1"
                     /translation="MKSNSTQIIFYFCFEGFSRLKDINNHIRETVSWLAKFGYEVHFF
                     NPNIVKPSFSSKVHIHLIPVINYPFLRWATFEFLSFFILTWNALKRRPTHLYFRETSS
                     FAPLLVSKLCHIPLTIEVNGWVLEELRQIGYSRWKLAYIRLNQRLNFVHCNRIIPVTE
                     GLKNLIVTNYPVKSVKIIPIANGTNPEVFRPMPKLEALAKTGLPNTPTVGFIGSCYHY
                     HGVQYLIKAAPLVLQKMPQVRFVVAGDGAQLEAWKTLSRELKVNEAFTFTGKVPFSLA
                     PYYINSFDICVAPWDTALVPKDGQSPMKFFDYLACGKPVIASPVSSVVKLINKFRCGI
                     TCDVKNSDVFAELIIELLSNPKIRESLGKIAREVVTANFTWEITSRKIEQVLKEIR"
     gene            complement(4594..5742)
                     /locus_tag="PKX09_02110"
     CDS             complement(4594..5742)
                     /locus_tag="PKX09_02110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU16649.1"
                     /translation="MKILVLTDLFPTLSNPNHGIFIYQWAYYLAQSCDLTVYQIVFKE
                     NQRSVSELELKRFRENYNANQRFAWTQRIKTIGRFDRIWRRSRQFYRQSKLDLSQTIG
                     EFDIIIGQMGCPGGYAAVRLAKKFGKKSIVGLRGSDVNAYLSRPVLKHYMHWTLKNAD
                     ILVTVSEAMKVELIKRGYPESKINVIYNGVDEKLFHPLDQAEAQKKLKLPPDYKYLLF
                     VGTLSQRKGINLLLSAFAELNRPEVKLILIGQSNDRHLIENFRIEHSLQDRILLSGVI
                     PHAQLPLWYAASDVVVLPSLSEGVPNVILEALAIGKPVVASQVGGIPEVLDGNKAAFL
                     CQPGDLNSLFEAIKKALNYSSKIEPFHKFTWEMNTKKYLQIFQEVIRR"
     gene            complement(5739..6896)
                     /locus_tag="PKX09_02115"
     CDS             complement(5739..6896)
                     /locus_tag="PKX09_02115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU16650.1"
                     /translation="MKQINVLHIIGNLQLGGAEKSLLLLLQNIDRAKFNPVVVTMYSD
                     GLNDFFSDDFRKLNIPLYHLHLKSWRDIETFRGLRQIIWRHSIDIAHSHYGALEFFGT
                     LYARLAGVRHCVYTKHNLRFKTSLLFKLGRILLNRILAERILSISKTVTEHLVNTEYA
                     SRSRIRLVYNPVENPKIPNAAHQASIKQKWQIPTGQFIIGNTSRFDSSKGFDIFYLAL
                     KYLQKMGIPVHTVVMGNESSQMEHQRLISENDLHELITILPYQKDLSPVYSILDCYLF
                     PSIHTEGFGIALLEAMSYGLPVVGLNVGVMNEIIRNQSNGLLPYPEKWEKTFHGNRTD
                     AAKALAKAIAELYNSPVLLRNISANAKVTAEKYSVTNFVNKLEAVYLELFK"
     gene            complement(6893..8821)
                     /gene="asnB"
                     /locus_tag="PKX09_02120"
     CDS             complement(6893..8821)
                     /gene="asnB"
                     /locus_tag="PKX09_02120"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOU16651.1"
                     /translation="MCGICGIYHHGKIEVSPAEICRMRDFMIERGPDSSGYELLPEIA
                     LGHRRLKIIDLSDAATQPMTNADRSIWTVFNGAIYNYRELRQELLAVGYHFKSNSDTE
                     VLLQGYAEWGTDLFQKITGMFAIAIYDSQKKQLILARDRVGKKPLYFTEQSGIVYFAS
                     DIKAVVAVLPAQPPVSLTALDCYLHHIAIPDQHTIFEGIQKVQPGSYHVFSENGQVVT
                     TYWEWNYAHKVVNDEPIILQNCEQLLIQAVLRRTVCDVPIGMMLSGGVDSSIITAILC
                     RNSTQRIKTFTMGFKNYPMEDILAAREVAKLYDTEHTELILDHDVTENLLELVWQFGE
                     PFADSSAIPTYLISRAARQYITGMLTGDGGDEAFGGYGRTIVPRNAQIFSQIIPPLLH
                     PLVSGILKSLGINPESNSIFGKILFYINYLKGFPYESFYNTMGFYKYRNSLWSPEYLS
                     KIVGHNPLHPFHENFRKVSDLDPIDQVLFTDGMTRLVYDYLVKIDRATMANSIECRSP
                     FLDTALLEYVAKIPPKMKFKNHQTKYLLKKIAEKYLPHKLIYTEKRGFGVPVDRWIQN
                     DLSGIFETVVFNRYARQRNYFNYDFINKIWNGQRTNVQNHKHRLWALFWLELWHLMFI
                     DKIISRDMPLREVERILK"
     gene            complement(8831..9508)
                     /locus_tag="PKX09_02125"
     CDS             complement(8831..9508)
                     /locus_tag="PKX09_02125"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU16652.1"
                     /translation="MKESISRILKYASQRREPFYEIVKKYVKPDSIILDIGAGNGSFA
                     EFIGRNDIFMIDGNLETVTRNQKRFPNYQYSRLPDLPFHNDKFDIIHCSHVIEHLTSN
                     DFFLLLKNCDRCLETSGYLIFSSPLMWYHFWDDLSHVRPYSPRIFEKYLCEKSSPNST
                     REKISNKYSIIELQYRYHLTEIGADVINIRSSIVNQVYRIVMQFLTRVGFRRIEKTGY
                     TIVLKKS"
     gene            complement(9517..10725)
                     /locus_tag="PKX09_02130"
     CDS             complement(9517..10725)
                     /locus_tag="PKX09_02130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU16653.1"
                     /translation="MTPGTNIVYLCANPGLDLDKMLGPKVHIQAILRGLKSAGVNPTL
                     IAVQNTKSLKEYEEFETVILPHHYLRGFVHRFIPYTGIVDSLRIFLKIIALNRTKHFT
                     LIHERYTGLSWGGVLAAKFLRIPLVLQMVGPGIEEKTLQLNPLKPSRRWLALLNQKYL
                     FSNCDHLILVSKLIASFIYQKRGWILPKHSVILFGPDLLDPLSEAKKTTIRRQIGAED
                     RPLFIYPGSLYRWYGTLDLVKAFHLALQKRPDLKLIIVGSGDEENTIREYIQKKSLTD
                     SVLLIGVVPHAELLKIIQAVDYCLVFYPGEPTYFGSSIKVIEYMAAGKPVISTPQMIE
                     IIEDGVTGFMSKTASPEDFAAKIEEVVSKPDLARTVGKNARNLISSHYTWNHYIEKLI
                     KIYKATLNQT"
     gene            complement(10728..12224)
                     /locus_tag="PKX09_02135"
     CDS             complement(10728..12224)
                     /locus_tag="PKX09_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOU16654.1"
                     /translation="MADSVIEQAKSGVRWVGIGQVGYRLLSIASSIVLARLLMPDDFG
                     VIALARLVLGFVMLLSAWGIDASIIVEEKRPKQIANVAFWINFGIMLVLGLLVIGISP
                     WVKKFYDTPVLQPILIWMGLGLIFQSFELVPRTILNRDLNYKYLTIINLSIEIFTQLL
                     VILLAIFGCGVWSLVIPQILASPLRAIPYWIKTKWRPSLYIEKADIKQVLSFGKSILG
                     AELIRYLNTNTDYFLIGKLLSKDKLGYYSFAFNLANWPVENIVKVINTVSLPTLAKVQ
                     SDLAEMRRLFLRMTEMVSLVTFPIFGVLVGITYELLVVIFGTKWIPAVRPLQVIVIYG
                     IMRSLFSPAGRIFLIQKKTHYMFFINLAQFPFLVVAVWLGIRKAEIIGAAVGVTIVLS
                     IGGLITIIVVSKMLKMKITEFIRTISPGFLCCVIFLICGWFFKHLLIGLGLPIVLILL
                     FYLLFAGSLYALSLRLFYPRLFIDFVRTSISIIGITPRNVFEKLRGNQ"
     gene            complement(12307..13608)
                     /locus_tag="PKX09_02140"
     CDS             complement(12307..13608)
                     /locus_tag="PKX09_02140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU16655.1"
                     /translation="MKYFSRIQNLLDKYEIPIIFAILLIGLVIRLAVIITIEEPIDRD
                     ALEYYTIAKNLNEHHRFSIDGENPTIRRSPGYPFFLAFFMKIVGTNPNHLYTVQALIN
                     ILTIWIVYLALKQIGANATIRIIAILLFTFNPSFIYVNTFYAEILTMFIMSLLIYLTI
                     NQELFKNKNWNIILQGILIGVLALIRPTFLYLPIFILVVSLLIRIFFHNKKMKDALIT
                     AIIAILTLVPWTIRNRITFNEWIPTESSSGLALWQANLEIENRTVWYSVTDIAKYEQQ
                     RTASAELQSKLRSEYLQKYDINSLVELNQFLEQKAIEIILRHPIRYCILCVNRFLIFW
                     FSPPIGSTTLKAISPVIFVLALLFRYLITILGICGLWQFARRDFPASFVLICLVVYLT
                     ILHSATHAIQRYFLPLIPLIYFALGYFLNNQKSLNRKGRQE"
     gene            complement(13640..14224)
                     /locus_tag="PKX09_02145"
     CDS             complement(13640..14224)
                     /locus_tag="PKX09_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOU16656.1"
                     /translation="MKNQEAEGLLSPLIKKIRLKKAAKCIQNNSTVLDLACGAGYLRQ
                     FLPSQCKYFGIDRIIPPSLNLFNEFLQLDILQPESFETIKEWLPEPKVDVVTMLAFIE
                     HIKNPQYLLKQSLLLLKPNGLLIITTPHPIGRKLHDSLAKLYLCSLSGAKEHENFFGQ
                     QDLKFLLKQSGYKLLSYERFLMGLNQLIIATPLK"
     gene            complement(14291..15229)
                     /locus_tag="PKX09_02150"
     CDS             complement(14291..15229)
                     /locus_tag="PKX09_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU16657.1"
                     /translation="MNLIIQIPCYNEEDTIEKTLNDLPRSIPGIDSIEILIIDDGCQD
                     RTVEIARQWGVNHFVHFNQNKGLARAFEAGLEKALELDADIIVNTDADNQYKGSDIVR
                     LVQPILAKKAEIVIGDRQIDKIEDFSWLKKKLEKFGSYIVRKVSQTNIPDVTSGFRAY
                     SREAALHINIISTFSYTLETIIEAGQKDIPIISVPIETNKTERPSRLFKSNWYYIRRS
                     FATIVRIYTMYQPLRVFFYTGITSFGIGLILSLRYLIFMIVGQGKGHIQSVIIAAVFL
                     IIGFFLLMIGLLADVIAANRKLQEETLYRIKKDSLK"
     gene            complement(15351..16484)
                     /locus_tag="PKX09_02155"
     CDS             complement(15351..16484)
                     /locus_tag="PKX09_02155"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU16658.1"
                     /translation="MIKYFRDLLSDRIRIENFRQAIESTVNHNSIVVEIGSALGTYSF
                     FAAKAGAKAVYAIEMNDIFYVGRELAERNNLTDRLKFIHGRSTEIDLPEKADLIIIED
                     FGPFFYYHNLLEVLRDARERFLKTDGRFLPARIILYIAPFECQAWRDELDLFAADNDQ
                     LFGFDWSYTTEIAFNQTYYADSHPKKLLSIPLNVKTIDLKNETNFTFQKQFELDTQED
                     GQIHGLIGWWDCWFTDQQFFSNSPQSGNNSWGQLIFPFRYPINVKSGESVKISLMVLE
                     SSPSGLIDYKWKITHHSGSQEQDTFSGRFFDLQQFNQMRRDATPSLNEKRLIQTFILN
                     QIDGKRSWDEIAEIVMKMFPEKFASKEDVFKIVLPLSNFLKGN"
     gene            complement(16486..17334)
                     /locus_tag="PKX09_02160"
     CDS             complement(16486..17334)
                     /locus_tag="PKX09_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16659.1"
                     /translation="MPLFKIQVGSAEISLECCQSTISEVTSSVFSAIITPSQISAKIH
                     YRIETKNGKYFLFRNNRLTHRNRQLSRIIYALEWQIVQDLLTKNKSALKFHGAALKHL
                     DTGFIFLGAASSGKTSLAILLMRYGWEFLSDEFALVDCDRQIIPCPRNLIIKPHLRDK
                     INIPLDSPIFKLDDESDGKIDAHYISPSLFGTVYLSKIPLKGLVFLEKADRAGFDLQP
                     LEHHKAFRLLTQHLLNRNFSGEKWLDFLVNLLDMSAVYRLKISSPIDLTPSDSRALVE
                     QLNQIE"
     gene            complement(17334..17615)
                     /locus_tag="PKX09_02165"
     CDS             complement(17334..17615)
                     /locus_tag="PKX09_02165"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOU16660.1"
                     /translation="MGKIDLDFKPTTRPDLPFRELKDGGVVYEYRTDTLHSLNATAAY
                     IWVLCDGQHTVAEIITSIKQNYNQFETDPTSEVIKIIEKFRTLNLLTND"
     gene            complement(17621..18979)
                     /locus_tag="PKX09_02170"
     CDS             complement(17621..18979)
                     /locus_tag="PKX09_02170"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOU16661.1"
                     /translation="MLVALVLVKVDPVYAILAAVGLGILIVTFTNTDFAIYILIFSML
                     LSPEIGSRTTSGEGMTIRIDDLILAIIILTWLAKTAINKELGLFRKTPLNKPIWIYTL
                     VCILSTGIGMMAGRVNFLTGLLFVLKYIQYFMIYFMVMNQIRTKKQINNYLISLIITF
                     TIVTIFALIQAPSGQRITAPFEGESGEPNTLGGYLVLLIAINLGLVLNDDAIANRHLR
                     RGLLLITVISCYPLLMTYSRGSWLAALAVIIAYIAISKKRWIIFGVVVLVFVLAPYIF
                     PQSVIDRVKYTFTEQQGYDATLQERIGGLTIDTSASERIRSWRAAFRDITKRPVLGFG
                     ITGWRFLDAQYIRVLIETGIIGLAAFVYLQFSLLSNIRKVYSDAKTPLYRALAQGFFV
                     GMIGMMAHSITANTFIIVRIIEPFWLACGLVMAVPEVEELEAEKLPEIEKSPTTINLE
                     NR"
     gene            complement(19050..19397)
                     /locus_tag="PKX09_02175"
     CDS             complement(19050..19397)
                     /locus_tag="PKX09_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16662.1"
                     /translation="MPHHKHHKSRSNRKAIIFLVIPISLIVAITIIIIIREPNWPFLS
                     ILRQDETAHQEKYMGSAVRVDTPIRQKYETEWRKDYFETWQKTYEKIMQNTDKNEIKE
                     LERELKASEKGIN"
     gene            complement(19409..22597)
                     /locus_tag="PKX09_02180"
     CDS             complement(19409..22597)
                     /locus_tag="PKX09_02180"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOU16663.1"
                     /translation="MAHYELNLRDYWRIIKKRKIIIIVTLVLLTCFSLIFAIMNRPTP
                     LYRATASLKIEKSTNLTGLYMYSISWGTGDDLATRAEEIKSYPMIEKAAQKLGLIDSS
                     LTSEQIRSNKEYLDLVSRLKSRVKTEQEGYTNIINIIVTDYDPVLAANLANALAQVYE
                     VESFKEKNLQAQKALEAVRNQRAMAEQALRISEQKVQNYREQNKLATLDGAASRLTAE
                     LNSAETNLEKIRGDIHQIDNILREIGNNREYIYLANLNLLLSQPNRFLEDLQTQLNQI
                     RSQITNYSQYYTPQHPAIIELQQQLKNTENRFINELKSFRQNLARAETIAEEKYKRLN
                     EEYKSLPLIELTLTNLERERDVNTQIYQQLEIRYQEAIIRYSEQVKEVFIIRPAFVPT
                     SPINQTTVGPTTAIGMIIGLILGIVLAFVAETLDTTFNTVDDIKEALNTNIMGIIPFV
                     DFQGLKSQLAEKAETPVPDEILDIQARLVSYYSPKSSIAESFRGLRTNVHFALLDKGY
                     KTLMITSSIYNEGKTSIAANLAISMAQIGINTLLVDADLRKPRISKLFGIDREPGLTD
                     VILRKEPIDNVIRTMSDLMVGTMAGDILRTDNIAGIEYLNILTSGKIERNPAEIVASK
                     LMDNLIAELKRRYDLVIFDTSPLIPATDATILGTKVDTVLLVYYQGKISRGTLRRSKS
                     QLELLKTDILGVVVNGMKAELSADYADYKYGYEYHYQEESAKPKNKVITALESFFLKP
                     AVGMTNGFFSQLNKLRAGLALIALGLIIGGGLWAFTKYQGKKAKSQVESKITETIIPQ
                     TTSSSDTSVASHILSSDTLPNLTAQAVPIIPPELKQLQEQYGIETISQQQVDQNLTES
                     GRPTNNSPTPVQSTSFATTQSDKQTEPLASIVYSSTTSSKTAPAVSVQPLPSSFPYTL
                     IIEQSFDKSTLINQLNKLRRNGLLAFITIDFSDPKQKNYLLCYGSFSSENEAINKSKE
                     LQFLGFRGTFRAIKLPFSILLDDFPTLQSAQQAIERFASIKDYLYIQPSSQKPDAKKT
                     YYLLFGGFPSTESTELVMNTYPELGHKTIVIR"
     gene            complement(22611..23858)
                     /locus_tag="PKX09_02185"
     CDS             complement(22611..23858)
                     /locus_tag="PKX09_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOU16664.1"
                     /translation="MKRNQFLKLPQKIILLVCISSLFGFAQKREPSITTDSYQNEMTL
                     SPGDRIKVSYIDIGPQGVPIEKSSILEVRHDGTIFHELLKVVYVQNLTIKQVEDLLTV
                     KFSEFFTQPQVVVSIVEKTTIKVFLWGGVDNIGVYRISPGTTLAEFIITHGGLTSDAN
                     ISKITLTRKDGSVIIFDLERYLFSNDETNNVELHDEDKIIVPRLLLKDEYARLSKGYI
                     LQAGNVLEITINELATMEKSPATPENYIIDQDGNIYHRRFGLVQLGGLTVDKAQATLT
                     ELAKKYYREPVVTIDVVKLTSRSVFVFGEVNRPGIYPIEGNIRLAEFLADIGGLTSNA
                     DPRKIIVTRENYKPVTFDLNEYLFDRKDEKNIYLEDGDRIIVLSRKRGFIVNLAEKIQ
                     PFTTVLYIISTALNIYFLITYTK"
     gene            complement(23912..24298)
                     /locus_tag="PKX09_02190"
     CDS             complement(23912..24298)
                     /locus_tag="PKX09_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU16665.1"
                     /translation="MEKILIVDDEQEIAELLEEVLEQLGYKTAMAFSKAQASEVLREF
                     EPRIVLLDIRLPDGDGISFLKEIKASQPAVEVIMITGLADRDIAIEALREGAADYICK
                     PIDLDYLANSLLANIVTRYNTDAPVL"
     gene            complement(24370..25536)
                     /locus_tag="PKX09_02195"
     CDS             complement(24370..25536)
                     /locus_tag="PKX09_02195"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU16666.1"
                     /translation="MIPRKNLARAFQKAIEQPDYALNNFIHRLKASFSYHFLNGNSLG
                     PETISLFLTYKCNLKCAMCGQWGTNGAFREYPSEVVRQQLSLDEIQNLIDDVRHYNPN
                     ITLFGGEPMLYPNWLKVVEIVKAAGLRCNIVTNGTYIGKWVREIADSRLDEIIFSLDG
                     PPEIHDKIRGVPGTFQKAINGLQELNTLKKTRNLNRPLVNINCTITELNYRSIDEIIK
                     IAEDIGACGLTLHHLLFLDRATVMEFIPFFQEKFDQTPTDWLGFIVDELPQIDPEYLI
                     AKRNELKTRKFKTDINFYPNLEDDEIRQWYTQFQFKASSYKNRCQSLWATAYVFPDGS
                     VRPYHSMNFTPGNILQDKFSKIWNNPSYIAYRRFVRKNKCFSICSKGCTEFFRY"
     gene            complement(25533..26300)
                     /locus_tag="PKX09_02200"
     CDS             complement(25533..26300)
                     /locus_tag="PKX09_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOU16667.1"
                     /translation="MRELKFKPGDVIINEGTYGSEAYILKAGSVEVSKMTDHKKVILA
                     ILQPQEIFGELGLIDDKPRSATVIARTAVTVDEITREDFSALLDDKMPFVIPIMRSFF
                     EHLRQANQLVLQMEAQITEASKTAPQPNAPTEIKLEGLTPIAKQALKNHAVNLRKFPF
                     KIGRESGQRREDVFIDNDLSLIDNIPYNVSRNHMSINFYNGQYYILDRGSSLGTIVNK
                     IPIGGRLSNFKAELHKGENTVILGTSNSQFQFKITIE"
     gene            complement(26499..27860)
                     /locus_tag="PKX09_02205"
     CDS             complement(26499..27860)
                     /locus_tag="PKX09_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HOU16668.1"
                     /translation="MTDKRLEEYRQALIDLRNGKTPRPINCEPDDELSALGREIIRFQ
                     KEWQELSLLIGLTAQINAGLNLEEVLDRIFNSFHAIIPYDRIGVALLTDDGKNVRSCC
                     TRSRAPVIKLKEGYSARLKNSSLEQIIRTGQPRIINDLEEYLAQKPRSTSTRLILSEG
                     MLSSLTCPLIIQTKPVGFIFFSSCNKNTYRDAHIELFQKIADQVSVIIEKSRLYQNLA
                     ALNEEETKFLGIAAHDLRSPIGVIKSYTDILLAGLVGNLTDQQTNIIKTIENNCTRML
                     NLINDLLDISAIESGKLTLQKTTIEPSTFFANYAENATRLTAVKSIHFTTFIAPQIPP
                     IEFDPNRITQVLDNLVSNAIKFSSPGTTITLTVKATGKYLEIRVADQGQGIPAAEIPK
                     LFKPFSRTSVQPTGGEKSTGLGLAIVRRIVQAHGGKIRVKSQPGQGTEFIIHLPFNPK
                     SDD"
     gene            complement(27869..29581)
                     /locus_tag="PKX09_02210"
     CDS             complement(27869..29581)
                     /locus_tag="PKX09_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOU16669.1"
                     /translation="MFLPTTKQEMEELGWQELDVILVTGDAYIDSPHIGIAVIGKVLL
                     KAGYRVGVIAQPALNVPDDIMRLGEPCLFWGVTGGSIDSMVANYTASLKKRRHDDYTP
                     GGINDRRPDRAVIVYTNLIRQYFKKTKPIVLGGIEASLRRIAHYDYWSDSIRRSILFD
                     AKADFITYGMSERSILELAESIQKGSDYRDIRGICYIAPHKPENTLEIPSYEKVRDNP
                     KTFTRMFHEFYGNNDAMRARRLCQKYADRWLVHNPPSLPLSQAELDEVYNLNYERDVH
                     PYYAKQGSVKALETIQFAVTTHRGCYGECNFCAIAIHEGRTVQWRSPESVINEVRSFA
                     TMPNFKGIVADVGGPTANMYGFECPQKLKTGGCKQKRCIYPEICPQLPVSHKKQIELL
                     IKLRDLPGVRKVFVASGIRHDLVLADKKWGRKYLREVISEHTSGQMKIAPEHIVPQVL
                     HLMGKPNHKTLINFIKLFYNYTRAANKKQFLTYYLIAAHPGCTYQDMVKLKSFATNFM
                     KITPEQVQIFLPAPSTYSALMYHTGFDPFTGEKIFVAKTLHQKEIQKNVVTSKREYPF
                     SKTE"
     gene            complement(29783..31060)
                     /locus_tag="PKX09_02215"
     CDS             complement(29783..31060)
                     /locus_tag="PKX09_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOU16670.1"
                     /translation="MSRTNSDSKKIPKVIVVGGGPAGLMAAGQASLSGAQVFLIEKME
                     RPGRKLAITGKGRCNLTNATELSEFLTHFGRNSRFLRGAFHRFFVPELLEFLKQLGIP
                     TVTERGGRIFPVRNDANEIVTALVNWANATGVIIRPNTRAEQLVLSENRISGVIVRNP
                     NSTTEKLNTDAVIIATGGASYPGTGSTGDGYRLAKSAGHSITPVRPALVPLVTVGNLA
                     ACLQGLSLKNVGIKVFADSRKIADEFGEMLFTHFGLSGPVILTISKLVVDALNAKQKV
                     RISIDLKPALDDQKLDLRLQRDFAQHGKMQFKTVLKELLPQKLIPVCLDLIKIPPEKT
                     CNQITTAERRRLRLWLKNFNFDISGHRGFSEAIITAGGVALNEVDSQTLQSKIVQGLY
                     FAGEILDLDADTGGYNLQAAFSTGWLAGQSAGR"
     gene            31513..33012
                     /locus_tag="PKX09_02220"
     CDS             31513..33012
                     /locus_tag="PKX09_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HOU16671.1"
                     /translation="MKRKIGIVIGGILLVALGFTIASIIIRPGKVVFYEDKNSGMTKF
                     RPAAVTAGDDIPSLQAFNDAFVQIAESVTPSVVTITTEKVIKTNLRRDFPGFPDDDLF
                     WRFFGFPQGEMRSKALGSGVIVDQKGYILTNNHVVEQGEKISVKLIDNREFKAEIVGT
                     DPLTDLAVIKIDAKDLQPAILGDSDALRVGEWVLAIGSPFSENLAHTVTAGIVSAKGR
                     SGVINNTNYESFIQTDAAINPGNSGGALVNIRAELIGINTAIATAGMSQGNVGIGFAI
                     PINLAKKVMTDLIEKGKVVRAWLGVVIGDVDENIAKSLGIESRQGAIISEVVKDSPAE
                     KAGLKVGDVIKEFDGKTIKDSAHLRTLVSTSEVGKPKTLVVLRGKKELKIDVKLGELP
                     ESVERGVYPDTKSDSKLGIRVDEINSVTASQYGISPDETGVVVTGVDRNGRAAGILQV
                     GDIIKRIGERDISNVAEYQKALEEVKTDIVLILIKRKNVTFFETIDLSK"
     gene            33187..33408
                     /locus_tag="PKX09_02225"
     CDS             33187..33408
                     /locus_tag="PKX09_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOU16672.1"
                     /translation="MNQVGKLLIIFGVVLIVVGVLLTVAGKFPFLDKIGRLPGDIIIR
                     REHFTFYFPWVTCLLISLIFFILFNLFRR"
     gene            33423..36563
                     /gene="ileS"
                     /locus_tag="PKX09_02230"
     CDS             33423..36563
                     /gene="ileS"
                     /locus_tag="PKX09_02230"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOU16673.1"
                     /translation="MIKKVPSSVDFVAIEHEILKFWEQNQIFKKLVAQNTNGPKWSFI
                     DGPITANNPMGVHHAWGRTYKDIFHRYKAMNGFRTRYQNGFDCQGLWVEVEVEKELGF
                     KSKRDIEKYGIEKFVERCKERVRKYSKIQTEQSIRLGYWMDWENSYYTMSDENNYTIW
                     LFLKKCHENGWIYKGHDVMPWCTSCGAALSEHEIATEGYKEITHPSITVKFPLINRPG
                     ESLLVWTTTPWTLTSNTAAAVHPDLNYLKIRQGNEILYVVEGRERDVIKGEYTIEGKI
                     PGREMLGWIYHGPFDDLPAQKNVKHVVIDWEEISATEGTGIVHIAPGCGQEDHELGKI
                     HDLAVLAPLDEFGNYLEGFGWLTGRNVDGISRDIFKDLEAKGIKYRTDSYLHRYPVCW
                     RHGTELIFRLVDEWFIAMDGVRQPMMDITRKIRWIPEYGLQHELDWLRNMHDWMISKK
                     RYWGLALPIYECQDCGQVTVIGSREELKEKAMEGWDKFEGHSPHRPWVDNVQITCPRC
                     GAKVSRIPDVGNPWLDAGIVPFSTLHYTTDRVYWEEWFPADFICESLPGQFRNWFYSL
                     LAMSTVLENREPFKTILGYALVKDEKGQDMHKSAGNAIWFDEAAEKMGVDVMRWLYAD
                     HNPFTNLNFGYNIAQDYRKRLITLWNSYSFFATYAELDGYSPQNHQVAEENLSDLDRW
                     LMARLVQLIRLSREALDDYNVARVMKGVDDFLEVLSNWYIRRSRRRFWKSDNDQDKWS
                     AYQTLYKALKGLILILAPVVPFITEKIYQNLVRGLEPDAPESIHLAKFPQAEEEFDEA
                     LLKKIDTIIEIVSVGRAARNKANLKVRQPLAQMWVKLPAEVTANDLVDLTDQILEELN
                     VKALDFITDETQFVNIQIKPNYAQLGAKYGQRLPAIRQAIAELNPIEVARRLEKSENL
                     ELNLIDGKIWLSRDEVIVERKENEGFAIAESNGITVILDTKLTPSLLQEGLVRDFIRH
                     VQTMRKEADFQVEDRIQVNFEAPADFTAAVHNYIEYFCNETLAQKVVDNFAPGEYEKE
                     LKIENNKVRVSLTRIK"
     gene            36590..36988
                     /locus_tag="PKX09_02235"
     CDS             36590..36988
                     /locus_tag="PKX09_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOU16674.1"
                     /translation="MEAKTTKKAWSKEDLEYFKNLILKKREETLKAIERLEATTVSDN
                     TRNDNTAMDSAYAYHMADVGTDSQEREKAFLWLARENKFLSHLNSALERIKTGEYGVC
                     IECGELIMKERLEEVPHTQHCVKCKNKPKK"
     gene            37123..38103
                     /locus_tag="PKX09_02240"
     CDS             37123..38103
                     /locus_tag="PKX09_02240"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOU16675.1"
                     /translation="MNLEPAKIIKPCPDEGVGKRLDLYLSEKLPAFSRTKIAGLIKNG
                     KILVNGQISKPSYHLQAIDTIAVFAEPQPISSIQPEDIPLEILYEDEYLLAINKKPGI
                     VVHPGAGVRQGTLVSALLHYTNQLSSLGGSERPGLVHRLDKDTSGVLIVAKNDEVHWK
                     MSRLFAERKVFKQYRAIVYGVPELDEGIIDAPLRRSQRNRQIFTVDERGRPARTRFTV
                     LKDYRIMAYLAVVLATGRTHQARVHLQSIGHPVVGDPVYGGKPRQGSAVSAKDVALLK
                     KVQSEVRRQLLHAYLVRFQHPFFDKDIEIIAPLPDDFSRILNILEVGIEP"
     gene            38118..39047
                     /locus_tag="PKX09_02245"
     CDS             38118..39047
                     /locus_tag="PKX09_02245"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOU16676.1"
                     /translation="MTINFLRFLAQERGYSENTIMAYRRDLEQFFLFYAEYANQVPID
                     LNSVSKMAIRHYLGKLAESGLEISSISRKLASLKAFFKFAARQGYADSNPAALILSPK
                     AKKRLPVVLTEEEIAKAIELPDDNTLVGKRNRAILELFYSSGIRLGELIALNIGDLNF
                     SRQTLRVFGKGAKERVVPFGKKAAEALNNYLNMRKKEVGALELNSPLFVSSRGGPNSQ
                     PTGYRRISRSQVQKLISGLLQQASEQTHLSPHVLRHSFASHLLDHGADLNAVKDLLGH
                     SSLSTTQLYTHVRISTMKEVYQQAHPHADSGKK"
     gene            39167..39454
                     /gene="raiA"
                     /locus_tag="PKX09_02250"
     CDS             39167..39454
                     /gene="raiA"
                     /locus_tag="PKX09_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOU16677.1"
                     /translation="MNIELTTRHYEITDRAKKHLEEEMNNLKLYEDLITSCKVIMEKT
                     KDGESIEISIHVRGKDLIAQVASDDMIKSIDLAIAKIERQLKKFKEKRYAR"
     gene            39451..40434
                     /gene="hprK"
                     /locus_tag="PKX09_02255"
     CDS             39451..40434
                     /gene="hprK"
                     /locus_tag="PKX09_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOU16678.1"
                     /translation="MNQNDLTVGRIYHENRKKLGFLRINGDIGHDTVVTSSKLNRPGL
                     EISGFWESFDPHRIQLIGEGEFRYIKTLSGDKVEEIFEHFFSAKIPLMIFAHDVKPPV
                     CAVRLARKYQVALLATPVTTTLLVASLLDYLDRELAPSTIIHGSLVDVYGIGLLLTGP
                     SGIGKSEIALDLVGRGHRLVADDVVKIIRRADNIIIGSGVEILEHHLELRGLGIVDVR
                     NIYGIRAIRQQKRIEVQIELEEWDNTKNFERIGAQEKYNKILDVDIPIINLPIYPGKN
                     ITVIAEVIALNHVLKIMGTNAALNFQKKIAATIRQKTQISDLQNYLKKDLE"
     gene            40450..41424
                     /locus_tag="PKX09_02260"
     CDS             40450..41424
                     /locus_tag="PKX09_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOU16679.1"
                     /translation="MKAIIPAAGIGERLRPLTLTRSKVLLPVAGKPIIGHIYDRLALT
                     GIKDVTVIIGYYGEQVRDYSLKNYDFNFTFIEQSEQCGLGHAVGLGLTPEDGPVMIIL
                     GDVILELDFKGLSSRSENLIGVMPVDDPRRFGIVEMKDGKVTRLVEKPSSSTSNLAIA
                     GIYLIQRGALLKKAIDYIITVNIKTKGEYQLTDALQKMLEWGESFEVINIEHNLDCGT
                     RKSLLKTNAYLLNKNPQPPPAIPETVIIPPVYIGRDAVIKRSIIGPNVHLGAEVQVMN
                     SVLSNAIISDGAILKDVILDESLIGQKSQVSGIVQQIDTADYTVQKLS"
     gene            41486..42643
                     /gene="metK"
                     /locus_tag="PKX09_02265"
     CDS             41486..42643
                     /gene="metK"
                     /locus_tag="PKX09_02265"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017186814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOU16680.1"
                     /translation="MKKYSFTSESVTEGHPDKICDQVSDAILDALLTVDPESRVACET
                     MTTTGLVMVVGEITTKAYVDIPRIVRETIEKIGYTDPDFGLDARSCAVITSIHEQSEN
                     IALGVNETEKHEQGAGDQGLMFGYACDETPELMPLPISMANKLTMRLAEVRKKGILNW
                     VRPDGKSQVTVEYEDDHPKRITNVVVAVHHNPEIAHEKIVADIKNHVILPVCQGYLTP
                     ETKFYINATGIFAIGGPEADSGVTGRKIICDTYGGMGRHGGGAFSGKDPSKVDRSASY
                     MARYIAKNIVAAGLARRCEVQLAYSIGVADPISVYINTSGTGKQPEERLAEAVRAIFP
                     LKPAAIIKYLCLKRPIYLKTAAYGHFGREDEEFSWEKTDKVAELKQYFGVK"
     gene            42643..43896
                     /locus_tag="PKX09_02270"
     CDS             42643..43896
                     /locus_tag="PKX09_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOU16681.1"
                     /translation="MTGKIKDITLAPSGKLKIEWAESHMPVMMALRQKYRDSKPLKGM
                     RISGCLHVTKETAVLIRTLIAAGAEVAWSGCNPLSTQDDVAAALYDEGVPIWAWHGLT
                     TAEFYWCIEQTLLQKPNLTLDDGADLVFTIHNKFPELAAGVLGGSEETTTGVHRLRAM
                     AEDGKLLYPMVAVNDAETKWDFDNVYGTGQSSIDGILRATSILYAGKTMVVAGYGHCG
                     KGVAKRASGMGANVIVTEVKPTAALKAKLDGFRVMPMDQAAELGDIFITATGMKDVIV
                     SRHFEKMKDGAIVCNTGHYDCEINLKELATLTKTKQEMRPNCVKHELTNGRSIYVLGE
                     GRLVNLVAAEGHPSEVMDMSFANQFMTMIFLAQNGAKLAKKVYDISVEQDQEIAFLKL
                     QTMGVNIDTLTPEQVRYKTAYSEGT"
     gene            44056..45576
                     /locus_tag="PKX09_02275"
     CDS             44056..45576
                     /locus_tag="PKX09_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16682.1"
                     /translation="MLDTENGNPIKADQSGVLYYSFTDSTSMPIEINPTTFRIVPAMT
                     ISQTLDLRKNIKRNVNNTVNPINYIMKKAWDDSNNRVTSGVLDNSTGFTADNIPINAL
                     VLAAQLSDSFPNPIQIRVIAPNFQKNPGQSVVATLDLPVNSLQDSIMIELGGASLIGG
                     LLKEEIDSLEFNIEVQVLGELAEATSSLKQKLTTSLTVGQLALESFNGRVFATGETPP
                     ALVAKSLTGASDIEFDSARASLNIETFGGNFDSVYVQLSGKKVHGVETVSDTMTAINR
                     ADLKMDIGEVVSNLPDTVRVFVKVMTATADFERNTVFNAGVNVKYNISVPLRFKIPPQ
                     ITLASGNTTTYFIKDSTTRARLITSQNGADLDMTVENRTDLQGDLYFLISNFNFFPFD
                     TLGGNLPSDFILFDGTVCHLGDDTTVVEIDTLAVLEFPPAQFSENTLIVPGIKSQIFQ
                     ISSDAIGLLADTCYFKPYFELVNPDTSLATITATQSIHVRGFLDLYFVATDLKDSD"
     gene            45579..47033
                     /locus_tag="PKX09_02280"
     CDS             45579..47033
                     /locus_tag="PKX09_02280"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOU16683.1"
                     /translation="MKPKKLSKRLTRTLRSSNWLWQLILLGFFLYGLPRPLDAQYYDS
                     PINRALGGAGNAITRGIFCVGQNPANLGLTAQYRTYVYLLGINGYVTNNFYSLESGAR
                     FSEKDLTANGGELQREFLEKLPGGGYRLNSSVASPLPLVNFSVGNKAFTTSLIQISDY
                     YLSKPALDVIFGNWEKGTTYNLDLRCDAMTAVEYVYSIGIPFGKMSVGISLKYLQGLG
                     YYGLDPAYSTGQIAVDTSNFVLYGNGSYYFRESSHGRGYGADLGVAFEDLNGWTLGFS
                     VLNLGESVKWNTETALSKWLKDNGIDVLKMMGFQIKSFPVKNTDLKLDFEGESYFYNF
                     RIDSLDADALFRSENTYQDHFSDTKLIKQDSSAFRVQVPLVLKVGAAKQLRKDLLTAI
                     DFSMSFSDRLNLRKGWRTAVGFEYAYFPKMPLRVGLALGGISGWEFDIGSSVNLAALH
                     IDWALGMNRGLWVHSMQGFDFALATYLVRGGKKK"
     gene            47049..49307
                     /locus_tag="PKX09_02285"
     CDS             47049..49307
                     /locus_tag="PKX09_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16684.1"
                     /translation="MIWILSFIKELFLLKQFRLYSTIFFLSIGLICGCSYEPLAPPEM
                     PTYYQTINLPLADVSLPLADLVNPDNNIYGDSTKDQLYFQFSGNLDTVTLTENIFQIP
                     AVGNVNFSQNFSELSESQLTFSRSVTQATKLSEIITIPGILPSPNDLLIGEIARYELD
                     GQRYKYRVFDKDDIPYFERVDYLTVGSGTFQTEIDNQLPVDMDSVRITLRNKNGSLIA
                     ESFYENVPAGATLSDGTPGNLNGAQLFDSIEVIVTAVMAGSQGQPVTIPANTDPFIAI
                     KFDTNIEEIESFTGIPKPIVTTQNQLLPPSNNTIFKATIAQTTTSPLDTNFLDLTIHN
                     LMPFNLRMQVIFHNFYIRNEPLTIDTIVQSGQIVENPARLDGYIFRNPDSTTVVDSFL
                     VEIIATILPNAGEQVVTIPTDMGDASIDVNLYFARLKLDMLEGFFNESFDIPPMAIAN
                     IPTGLANIDFGQVFLKLTFFNEIQARTNVSLVLRGLKKGVAPQTITEDGSIAKASPAN
                     LIQESMMEIDIAPIFNMVPDSILVSGQAIIPSDDTSRLQIGKAFWGFYEITVPFQVKI
                     GPVTFIPVNSTEMAAIDPETRQKIRDGLIKSSIYTEIINDFPFSGNLSILMSNYDYFP
                     LEPDSLDAGYFWLNDTLYARTDTGDVPIIIDTLLLVELPPPEAFNSNGSVKTPGFTLA
                     ETPLDSAKMEAILRDETHYIRPRVHFNATEDFVIVGYYDEVRILAAISLTVETMELIN
                     NNNENETPATVP"
     gene            49350..50705
                     /locus_tag="PKX09_02290"
     CDS             49350..50705
                     /locus_tag="PKX09_02290"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOU16685.1"
                     /translation="MGWLNNRYKITLLLMLGMSIMATAQTRRDPRALALAGAYGSLAR
                     GIFAIDYNPANLAIPHEYHSYRIFGGFGTNVSTNFLSIKQYQKYNGKDLEAGNGHLKK
                     EFMNDIPDEGWRVFTDIYFPLPLISYSTYNRAISSDLIVIGDLGLPKDLLNFVFDENP
                     IGETLDLDFNEEVMAIIQWGYSLAFPVGNIFLGFTAKYLQGLAYIGLNPDSSYGSVTT
                     YFEPERTYIDGYGQYYFQQSLGGRGFALDIGLTTEELNGYRLGLSLTNLFGSITWNKS
                     TLLSRSVSAEKVLPWEGNFYKYVFSIDEARFDKFFGKASVDDVFQGEGYKIKDTTAFR
                     VKYPSLVRFSAAKILEENITLASDLVVGFEDRLYSFGAWKWCLGLESTKSQRFPLRVG
                     LSFGGHSSRELTFGSGFHWGLIHLDWALGFNHGLWPTTTTGLNFNMLCYSTKRAKTSY
                     R"
     gene            complement(50942..52027)
                     /locus_tag="PKX09_02295"
     CDS             complement(50942..52027)
                     /locus_tag="PKX09_02295"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOU16686.1"
                     /translation="MKLLDRYILKKFLLIFGLCLLAFLVIFNIVDLIEKLDKFLKADM
                     PSNLIALFYLYQLPYYLNLALPMALLLAAVFTIGMLSKHNELDAIKSSGISLYRVSIP
                     LLITGILISIGSFFFEDAVVIPATRRRIDIEAHNMHRRKYIPKTFFTNIMYQDSPTCN
                     IVIGNFSTKDNIAQNVTIQYNDQFRLVRRIDARKMVWLPDQQLWQLFDYKVRSFDQDG
                     TETVVAASSDTLIQLNLQPEDIVKSDLNPEGMRYNELAYFIKRLQESGNDARKWEVNK
                     HFKLAFPFTNFIVILFGVPLAAAKPHKGIAFGAGMSLLAIFIYYGFIKLGQVLGYKGI
                     LSPLLAVWLGNIVFLIAGGYLLYKIRQ"
     gene            complement(52267..52608)
                     /locus_tag="PKX09_02300"
     CDS             complement(52267..52608)
                     /locus_tag="PKX09_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16687.1"
                     /translation="MLNIIKNFVAILKIEIEDLAADIELMMNECQKKKDAGKITDYVY
                     LENMALYRHELLAVRSFARLLESTELSNFETLDALITYLQESFREAGYVRAINICVER
                     KMKKVAGYVSR"
     gene            complement(52697..54130)
                     /locus_tag="PKX09_02305"
     CDS             complement(52697..54130)
                     /locus_tag="PKX09_02305"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOU16688.1"
                     /translation="MHRFQLFSLIIFWASISNCSNPISPKPENPFKNINLQILSDSTL
                     AITWQGEFESEALLSLDRKVGEASWQMDYTNVPANDSIYVDTLYSVHTDTVYSYRLRF
                     KESDNSSSISPSVALLSERCVPVDFSARQIDKSTIRIAWRDQSIGEAGFRIDRQVGND
                     DWQIAYAKADSNSYEYFDITTTSSEISYRIAAYVGSSFSSFTEIVKIKLGSLTLANLY
                     FGTEPTFEVMTWNIEEFPRFDTLTVSAVSQAIKALDIDIIGMQEIKSATAFQQLLDSL
                     GSWDGYRTNSAAYGINLAFVYNPANITVNQYYEIFEHEDAFPRPPLVIELEWQNVPII
                     VINNHFKAQGDEDSQNRRLQACTLLEEYIRTNLADDNVIVIGDLNDELTDAQSVNVFW
                     PFLSQPSAYKFADYSIAQGSSKYWSYPTWPSHIDHILITDELFGVFANSTSVIKTLLI
                     ENYMEGGWNEYAVKISDHRPVALKLAF"
     gene            complement(54545..56911)
                     /locus_tag="PKX09_02310"
     CDS             complement(54545..56911)
                     /locus_tag="PKX09_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOU16689.1"
                     /translation="MPPKKIDNTQIPTSNVEKYYLSLSGRQFIYPPTEKIPVIQVANF
                     PMLGQLTALRFLEWVQNNPDGVIALPTGKTPEHFIKWVGYYLTNWHSAEVIKDLEMVE
                     IDPARRPDMARLHFVQIDEFYPIDPNQQNSFYYYIQKYYIKNFGLDPEKALLINLYEI
                     GTANNLSIAEIFGDEKADLSLRTRWAKNPIEELQKETIARTDQFCVNYESKIRALGGI
                     GFFLGGIGPDGHIAFNIRGSDHYSTTRLMETNYETQAAAASDLGGIEIARKRLVITIG
                     LDTITYNKDATAIIIAAGQAKANIVRDSIQNFPSIQYPATVLQKLPNARFYLTEGAAS
                     KLFERRYEKMAKSPELEPKTIEKAVINLTLEKNKKLTDLTLADFNSNRFTELILKRTN
                     QTHTEICRNLHESIINRIDRGIYYLDNEVFMHTSPHHDDEMLGYLPYIVHLVRNPRNK
                     HYFTYMTSGFTAVTNSYVLERLKNLQDLVDSPTFQVLMQEHYFDPANTAARNRDVNLY
                     LDGVALYDAKKKTEAESRRLLSILTEIYEENNVIYLKDRISELINYCTTQYPGKKDIA
                     HIQKLKGMIREWEADVLWGYFGFNSNNVFHLRLGFYKGDIFSEEPTLDRDVLPIYQLM
                     KRIKPTVITVALDPEGSGPDTHYKVLQAITETLKLYEKEEDISNLRIWGYRNVWHRFH
                     PASTNIYVPVSLNSMAIMENAFMESFGSQRSASFPSWEFDGPFCRLARKIQVDQYLKI
                     KTCLGRDFFIKNEHPRLCATHGFVFIKELTPKEFYRHSEESRRVTENL"
     gene            57291..57752
                     /gene="nusB"
                     /locus_tag="PKX09_02315"
     CDS             57291..57752
                     /gene="nusB"
                     /locus_tag="PKX09_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOU16690.1"
                     /translation="MRERRAAREVALQALYAEELTSYPIAQIIDDVIENSELQDELKT
                     FAKDLFIAAVTHRDELDEIIREKSENWDFERIAIIDRLVIRMAISEFKYFNDIPPKVS
                     ISEAIEIAKIYSTDDSSAFVNGILDAVLHDISDILTYKLKRSETKSATREE"
     gene            57834..60905
                     /gene="secA"
                     /locus_tag="PKX09_02320"
     CDS             57834..60905
                     /gene="secA"
                     /locus_tag="PKX09_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOU16691.1"
                     /translation="MGIATYLTKIFGTSSDREIKKILPLVAEINRIYATLSEQPIEKL
                     RERTAELKVQIQAAREAARIEAEESGISEEEAQKLIQTAEQNALNAILPEAFAMVKEV
                     CRRLVGQEWLVTDQKIVWDMIPYDVQLIGAIVLHQGKIAEMKTGEGKTLVATMPVFLN
                     ALTGRGVHIVTVNDYLAQRDSEWMGRIYTELGLTVGCILNSMDSEERKKMYACDITYG
                     TNNEFGFDYLRDNMALSAEEQVQRSYYYAIVDEVDNILIDEARTPLIISGPVQSSRHE
                     KFDELNPAVGTIVRYQRDKITEILSRFPDSPESIPEKDYSEFARQLFIASQGMPKNRR
                     LRKLLEEPGYQKMLQSGEKEYLLLTNAKTGTSISQDEFFKDLYYFIDEKNNNITLTPN
                     GEEKLATLLGIKLDELIIPDLAEEFVKIDENPELDEVEKEKRKMELDTQHSEISDRYH
                     NISQLLRAYTLFEKDVEYVIQDGKVLIVDEFTGRILPGRRYSDGLHQAIEAKEKVKVE
                     AETQTFATITLQNYFRMYEKLAGMTGTAATESAEFADIYKLEVVVIPTHKPCIRIDYE
                     DQVYKTRREKYNAIINEIETSFRHGQPVLVGTISVEVSETISRMLKRRGIPHNVLNAK
                     NHKREAEIVARAGERGAVTIATNMAGRGTDIKLGQGVVELGGLYIIGTERHESRRIDL
                     QLRGRSGRQGDPGASRFFLSLEDDLMRLFNSERIAAIMDRLGVEEGQVITHPMVTRSI
                     ERAQRRVEERNFTIRKNLLEYDDVMNHQREIIYARRNYALKGSDTKGELYNILEDWAD
                     DLVQKYNAQVKDSGDWQWMALRMDLTTSLGIDLDPSELSEYTPEALKQAIIDRAKQYY
                     ERKEKIYGAEQMRFLERFIMLRTIDEKWRDHLYAMDQLKEGINWRAYGQKDPLLEYKG
                     EAFDAFVQMVNDINQQTLRLCFRAQIGSPQPETRQVRRPATMRLMHQQADGMGFIPGM
                     QSETEEQPQYATSDGQQAKIRPVRVEAKVGRNDPCPCGSGKKYKYCHGAGN"
     gene            61060..61142
                     /locus_tag="PKX09_02325"
     tRNA            61060..61142
                     /locus_tag="PKX09_02325"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:61094..61096,aa:Leu,seq:tag)
     gene            61180..62448
                     /gene="tig"
                     /locus_tag="PKX09_02330"
     CDS             61180..62448
                     /gene="tig"
                     /locus_tag="PKX09_02330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOU16692.1"
                     /translation="MKAELINSTPIEKTVKVVVDWDEIADDYIAVFNDLRRDLKIDGF
                     RKGKVPESLAKRLLKPQIFVNFTNQIIEKTHKQALKDVGMDDYLDLHIAELDFNENTP
                     FNYTIKVENDPEITLPDYKKGIKIQQKEYIVDPETVERYIEDFLENQAEVQEVFDGAQ
                     DGDFLVADLQALDEGGIPIIGKRLPDRLIKVGEGIFGDKEAGNFKGAKAGDSVNLNVK
                     SSSGKTLKYLVDVKRVEKHTLPELTDELVKSQVEDVQTVAEFRQRITENLQYKWNQRA
                     QEELDQALADYFIENTKFDIPPYRINSYLDSLIEKMKENSQNKPIDEAKIREEYLPIA
                     EKNIRWYLIQQAVRKQEDIRVSEDEIQTEIDKILQIVPEEQKEFAEQFYQKSRNRLEI
                     KLNLEDRKIREHIAQFIKGEKVTVHTESLQ"
     gene            62461..63066
                     /gene="clpP"
                     /locus_tag="PKX09_02335"
     CDS             62461..63066
                     /gene="clpP"
                     /locus_tag="PKX09_02335"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOU16693.1"
                     /translation="MKTKAQAYVPFVIEQTERGERSYDIYSRLLKERIIFLGLPIDDH
                     VANLVIAQLLFLAAEDAKKDIYLYINSPGGSITAGMAILDTMNYIKPDMVTICLGLAA
                     SMAAVLLASGTRGKRQALPNSRIMIHQPLGGVEGQASDIEIHAREIIRLKENTNRILA
                     ELTGQPLEKINRDTDRNYFMSSEEALKYGIIDEILPARIKK"
     gene            63191..66169
                     /locus_tag="PKX09_02340"
     CDS             63191..66169
                     /locus_tag="PKX09_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU16694.1"
                     /translation="MARRVAICLLAMLLPIVLFAGTSGKIAGSVVDKNTGAPLAGVNI
                     IVTGGGYNLGAATDLDGYYSILNVPVGTYTVRSSYIGYKETVYNNVTVSLDLTTELNF
                     EMERTTLEFEQVVVTAERPMIRKDATNTNIIKSAEEIANMPLRGMQEIAASTAGVVKA
                     ENSTAMNIRGGRSAETALYIDGVLVNDPYNNAVYTYLPNEAIAEMSVQTGGFNAEYGD
                     AMSGIVAITTNAGSENYSASFQVITDEFLKADEKILGTYGYGFNEYVASIGGPIIPKS
                     NHTFFISGTRRYAADAWPSYGWAENDYRLDDFTYDQVLTRIDDQGNTFLDTITHNYDL
                     TARIPGNSTSDWSWNAKLKFQLGKNMVLKTSYVSTDRIISSVTPIDIFNTEHMGEESR
                     YSHSFNATLTHTIRQNTYYDLKFNWFDTKRKIYDATFGDDLEKYGDPWLNPWPDNSAY
                     VGVAYTGRITPDYFQPGCQYDDYFKNRTTYWGIDFDIVHQMGKNHTFKAGFEYKYHTL
                     REYRVLAPVYLAKTNLSDLEKYQSADVRFYGYDINGKEIEEGDFLEDAVYDETGRLAD
                     GYQKQAPYHPIILSGYIQDKIEFRDLVLNIGLRYDHINPNAWQFKQLDAEWAADGSYI
                     PGTGMFGGNEKFDKTDVEDSEVHTYISPRLGISFPVTDQTVFHAQYGQFYQAPSLQDL
                     YLSPFYLEAFVSRGGYFTTLDNPNLKPPKTTSYEIGFKQMLGNIASVQLTTFYKETED
                     LIQVMNVTTDVTNIAFSRNGDFGTVKGLDIIFNLRRYKNLAATFNYELQYATGTGSAT
                     NSNFNIAWQGGARGNFPKFAMPLDFEQRHTGSLNLDYRLGRTEGPKFLHNAGANLMFS
                     FNSGHPYTLMQVFNTMPFTGRYDNTNVSETPVSAVNSQTTPWVFRIDLKLDKKFYIGG
                     VQLTAYAWALNLLNTENVANVWITTGLPDDTGYLGTSAGRQYWNSLTDEQKSLYKMRE
                     MDYNHYGSPRQIRLGLMLEL"
     gene            66213..>67170
                     /locus_tag="PKX09_02345"
     CDS             66213..>67170
                     /locus_tag="PKX09_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16695.1"
                     /translation="MKKILSIFMIILTVSTLVIGAEDIYKTRALEKAAMTSDIWVNAN
                     RMNGVFRNNGTWFYDNVLGDWGLEWPKGSGLSPIFGAGQYIGAKIDGDIRVAGVQHSA
                     TEFQPGLILATDVADNKADPKYRWYELRSGGVGDWTTWPTDQGAPLDKNGNPMLIGDQ
                     TIYSVWNDLAPHSEYGTNKLNAEAHQMAWAFDRANVMGDMIFIKWTLVNKSGKDWEDT
                     YFVIWTDPDLGNAGDDQVGCDSTLGLGYCYNATDNDQVYGAAPPAVGIDFFQGPIVSS
                     DNPLDTVKLPDGSVFPGKKMLKMTSFIYYNNDDSNQGNPSSGM"
BASE COUNT        19289 a        13001 c        15299 g        19481 t
ORIGIN      
        1 aagttacaca tttttatcac ttatcctacc tctccagttt ttggggtaaa gctcaaagtc
       61 tccttccagg aatgattgat cattgtcaac cgcaaagcat gccaaggatt catcgcgcta
      121 actacataac tgaaaaatta gtcgtcagta atctttgcat acttggcggt caaaaatcat
      181 cttttatgat ttcggcgatt ttcagactgg cattgccgtc accgaaaaga atggtgtttt
      241 cacgattaaa aacggcaaat ttttgggtct cttccggtag tcgctggaag tcgtttccga
      301 ttaattttgc ccaaccgtat tcgacgagtt ctacccactc cgtttcaggt cttagcacga
      361 ggcatggcac tcttgcgaaa taggcttctt tctgcactcc acccgagtct gttatgatca
      421 gtttggattg tttttcgagt ttcagcatct ccagataacc gacgggctcg acgaaacgaa
      481 gattgggact atcggctgta atattaaact gcctcattag attacgcgtg cggggatgaa
      541 tgggaaagat aatgagttcg tttaagtgct ctaaagtgct aataagggct tttaaggtgt
      601 tggggtcgtc ggtattctgg gggcgatgaa ttgtgaccag gtaatatggt tgcgaattga
      661 tgccgagtcg ttccagaatg tctgattttt ccgcaatctg gctattgtat aagaaagcgt
      721 cgtacataac gtcgccgacg agatggattt gatcatcgaa tccttcatgg cacagatttc
      781 tgacagcggt ttgtgtcggg gcaaacaaat attgcgagag ggtatcggcg acgatgcgat
      841 tgatttcctc cggcatttgg cgattgaaac tgcgtaatcc agcttcgaca tgcgctattg
      901 ggatttgcag tttggcggcg gccagggcgc cagccatggt ggagttggta tcgccataaa
      961 tcaagacgag gtctggctga attttcagta atgttttttc caattcgatt agcattttgc
     1021 cggtctgttc gccgtgattg ccactgccga tctccagatt ataatccggc tcgggaatat
     1081 tcaattcggc gaagaaagcc tgtgacatat tatagtcgta gtgctgaccg gtatgaatca
     1141 agatttcgtc aaagtatctc ctgatttcgc gcgaaaccgg tgcgcatttg atgaattgcg
     1201 gacgtgctcc gacgatggta gcgatggtgg acaagttcgt tcctttcagg tttgttcaac
     1261 cccgttgggg ttgtatggtg tttcgcattt ttatggctca ggatataatc ctgagttatg
     1321 caggttcaac tcctgacgga gttacggatt gttcaggtct caggttcaac cccgttgggg
     1381 ttgtatggtg tttcgcattt ttatggctca ggatataann nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnntc ctgagttatt cgggttcaac tcctggcgga gttggagggt
     1561 gttcggggtt agttctttag gctcaggata taatcctgag ttatgcaggt tcaactcctg
     1621 acggagttag aggatattca tgtttcgagt tcaactcctg gcggagctgt gggttattcg
     1681 ttaaatccta tctcgactgt tcgggattat aattaaatca agtttatgaa taattattcc
     1741 gcttaatccc agcgggattg aaattgaata gaataaaatc atacatatcg cccaatccca
     1801 acgggattga atatgaaatt aactaatttt ggttaggttt tttacgacgt aagtaggttt
     1861 gtttaaccca cttccaatta agaattaaat gtccgatgac tccgagaatt aataagcctc
     1921 caaaaatttc gtggacttcc acaatagcgt cgctggtaac tatcccagtt tttaaaagaa
     1981 acagcgaaac tattatccct aaaaaggcaa ttcccatcag gcaattgaga tactttaagg
     2041 tagtttgttt tgacattgtt ggttactcct ttatttgatg attatggttt aagctcagat
     2101 atgaaatagc gcatcttgcc ggtttgatct ggttttattg cagtaactgg ttgaaattcg
     2161 atttggatgt tatcatcatg gactgtttta cgaagtaatt tttggaaaag gtctaaatcc
     2221 gaatcggaat aattatgact tttgacatag cgaatagtaa aagtcgatag ttgagtttgg
     2281 ataatttggt actgatcaaa aggttgccga ttcagatcga gtaaagccag attcaggtaa
     2341 tcgaaaattt cactcgaaaa agtttgccca tttttcatta caacaatatc agtggctttt
     2401 ccgatgtgca atttcataat tggcagattg caaccgcatg ggcattttcc gttaatccat
     2461 tcaccgatgt caccaatgcg atagcgaata aaaggcatat attcattagt cagcgaagtt
     2521 ccgacgattt cgcggactcc attttcattt tcgataaatt cgagaaaaac attttcaatg
     2581 gaaacgtgcc aattaccagc tggacattcg taagcaaaac caccgatttc tgaacaacca
     2641 tattcgttag aaactggaac gtggaaaact gactcaacta ttttgcgttc ctgtggaaat
     2701 agagtttctg cggttacgaa aattgctttt aagcgcccag gaagtggcag gttgttctct
     2761 ttataataga gtgcaagccg gtaaatgctg ttagagtatc cataaaagta gcgcgggtta
     2821 aagtgccaaa tctgacgact taatttttcc agcgtttctt ctgagtattc aaagcccgaa
     2881 attagcagtg tattccgaaa gcgtcctttc agggcatcta tgactttgcg aatagtcgag
     2941 tggagcggtc gcccccagat agcaacttcc ggatccccga tttgaactcc aaaccagttc
     3001 cgtgctcgcc accgactagc ccagtcccaa ctgctatatt cgttagtaat gtaaaatttc
     3061 aatggaatac ccgttgagcc actagttttc ccagaataaa ttttaccgga ataatttgga
     3121 tttgttaaac ggctgagatt tttcctaata tcttttttat ttaacaacgg aagatgggcg
     3181 aaatcctggg gcgaggagat gttctctggc ttgatcccaa gctggttaaa tattgcttgg
     3241 taatagggta cgttttgata agctacgtca agaatatgat aaagtttagc cagctggatt
     3301 ttctgcaatt tttccagcgg taaatgttca ttgtatctaa gaatttttaa atattgagca
     3361 attttctcac ctttaatgtg ctgagtcaag gggaaataaa agtcccggac aaattggtca
     3421 tacattatct aatttccttt aatacttgct caattttacg actagtgatt tcccaagtga
     3481 aattcgcggt gacaacttcg cgagcaattt ttcccaatga ctcacggatt tttggattag
     3541 ataaaagctc aataattaac tcggcgaaaa catctgagtt ttttacgtcg caggtaattc
     3601 cacaacgaaa tttgttgatc aatttgacca cgctggaaac cgggctggca atgactggtt
     3661 tgccacatgc caagtagtca aaaaatttca tcggcgattg tccgtccttt ggtactaaag
     3721 cagtatccca cggcgcgacg caaatatcaa atgaattgat ataatagggc gccaaactaa
     3781 aggggacttt gccggtgaat gtaaaagctt catttacttt cagttcacgg ctcaaggttt
     3841 tccatgcttc taattgagca ccgtcgccag caaccacgaa gcgaacttgt ggcatttttt
     3901 ggagaacaag tggagcagcc ttgattagat attgtacacc gtgataatga taacaacttc
     3961 caataaatcc gacagtcggc gtattgggca agccggtttt ggctaaagct tccaacttcg
     4021 gcataggccg aaagacctcc ggattcgtgc catttgcgat gggaatgatc ttaacagatt
     4081 tgaccggata attagtaaca atcaggtttt tcaaaccctc agtaaccggt atgatacgat
     4141 tgcagtgaac aaaattgagg cgctgattca aacgaatgta ggcgagtttc cagcgcgagt
     4201 aaccgatttg acggagttct tccaataccc aaccgttgac ttcgatagtt agtggtatgt
     4261 ggcagagttt tgagacaagc agtggagcga atgatgacgt ttcgcgaaag tataaatgag
     4321 ttggtcgtct cttaagtgca ttccaggtta aaataaaaaa agataaaaac tcaaaggttg
     4381 cccagcgtag gaatggataa ttaatgaccg ggattaaatg aatatggact ttagagctga
     4441 aacttggttt gacgatattt ggattaaaga aatgcacttc gtatccaaac ttcgccaacc
     4501 agctgaccgt ttcacgaatg tgattattga tgtcttttaa gcgactaaaa ccttcaaagc
     4561 aaaagtaaaa gattatttgg gtagaattcg atttcatctc ctgataactt cttgaaatat
     4621 ttgtagatat tttttggtgt tcatttccca ggtaaattta tgaaaaggtt caattttact
     4681 tgaataattg agtgcctttt tgatagcctc aaacagtgaa tttaaatcgc ctggctggca
     4741 tagaaaagct gctttgttgc catctaatac ttccggaata cctccgacct gcgaggctac
     4801 caccggcttg ccaattgcca gtgcttccag aattacgttc gggacgcctt ccgaaagact
     4861 cggtagtact actacatccg acgcggcgta ccaaagtggt aattgcgcat gcggaatgac
     4921 tcccgatagg agaattctat cctgtagtga atgttcaata cggaaatttt cgatcaggtg
     4981 gcggtcgttg ctctgaccga ttaaaattaa ttttacttct ggccgattaa gttccgcgaa
     5041 tgccgaaagt agtaggttga ttcctttgcg ttggctcaac gtcccgacaa aaagcaggta
     5101 tttataatct ggcggcaatt ttaatttttt ttgggcttct gcttgatcaa gcggatggaa
     5161 aagcttttcg tccacaccgt tatatataac gttaattttt gattccggat atcctctttt
     5221 aattagttct actttcatag cttcagaaac tgtgaccagg atgtcagcgt tcttcaatgt
     5281 ccagtgcata tagtgcttta agactggccg cgacaggtaa gcattgacat ccgaaccgcg
     5341 cagaccaacg attgactttt ttccgaattt ctttgctaat cgcaccgccg cgtaaccgcc
     5401 ggggcacccc atttgtccga tgataatatc gaattcaccg attgtttggg ataaatccaa
     5461 ttttgactga cgataaaatt gccgcgaacg tcgccagata cgatcaaagc gaccgattgt
     5521 ttttatgcgc tgagtccagg caaagcgctg attggcgttg taattttcac ggaaacgctt
     5581 cagctccagt tcactgactg atcgctgatt ttccttaaaa acgatctggt aaactgtcag
     5641 gtcgcaactt tgcgccaaat aatatgccca ttgataaata aaaatcccgt ggttgggatt
     5701 tgagagtgtc gggaagagat cagtgagtac cagaattttc acttgaataa ttccaaatat
     5761 accgcttcta atttgttaac aaaattagtg actgaatatt tttcagccgt tacttttgcg
     5821 tttgcagaaa tattacgaag taaaactggg ctattgtata attcggcaat ggccttagcc
     5881 agagcttttg ccgcatcggt ccgattacca tggaaggtct tctcccactt ttccgggtac
     5941 ggtaaaagcc cattagactg gttgcggata atctcgttca ttacgcccac atttaatccg
     6001 acgaccggca atccgtaact cattgcctct agtaaggcta tcccaaatcc ttcggtatga
     6061 atcgaaggaa atagataaca atcgaggatc gagtagactg gactcaggtc tttttgataa
     6121 ggcaggattg ttattaattc gtgaagatca ttctcgctga taagtctttg atgttccatc
     6181 tgactggatt cattgcccat taccactgta tgtaccggaa tgcccatttt ctgcagatac
     6241 ttcagcgcta ggtagaaaat atcgaaacct ttggacgagt caaagcgact ggtatttccg
     6301 atgataaatt gtccagttgg aatctgccat ttctgtttaa ttgaagcctg atgggcggca
     6361 ttcggtattt taggattttc gaccggatta taaacgagcc tgatccggct tcgggaagca
     6421 tattcggtat ttactaaatg ttcagtgacg gtttttgaga ttgataaaat ccgctctgcc
     6481 agaatgcggt taagcaatat cctcccgagt ttgaataaaa ggctggtctt gaagcgtaga
     6541 ttatgtttgg tataaacaca atgccggacg ccggccagcc gcgcgtaaag tgttccgaag
     6601 aattccagtg cgccatagtg tgaatgggcg atgtcgatgc tgtgcctcca aataatttgg
     6661 cgcaatcccc gaaaagtctc aatatctcgc caggatttta aatgcaggtg atagagcggg
     6721 atattaagtt tgcggaaatc gtcagagaaa aaatcgttca aaccgtcgga atacatcgta
     6781 acaaccactg gattgaattt tgcacggtca atattttgca aaagcaaaag tagtgacttt
     6841 tcggcgccgc cgagttgtaa atttccgata atatgcagta cgttgatttg tttcatttca
     6901 gtattcgttc gacttccctg agcggcatgt cgcgactaat gattttatca atgaacatta
     6961 aatgccagag ttccagccaa aagagtgccc aaagacgatg tttatgattt tggacattag
     7021 tgcgctggcc attccaaatc ttattaatga agtcatagtt aaaataattc ctttgccggg
     7081 cgtaacggtt gaatacgacc gtttcgaaaa ttccactcaa gtcgttttga atccagcggt
     7141 caaccgggac accgaatccg cgtttctcgg tgtaaatgag tttatgaggt agatattttt
     7201 cggcgatttt cttcaacagg tatttggtct ggtggttttt aaatttcatt ttgggcggga
     7261 ttttggcgac atattccagt aaagccgtgt cgagaaaggg tgagcggcat tcgatagagt
     7321 ttgccatggt cgcccggtct atctttacta aataatcgta aaccagccgg gtcataccgt
     7381 ccgtaaacag cacctgatca atgggatcga ggtcacttac ttttcggaaa ttttcgtgaa
     7441 acggatgaag cggattatgt ccaacaattt ttgacaaata ttccggactc catagagaat
     7501 tccgatattt ataaaatccc atcgtattat aaaagctttc gtatgggaag ccttttaaat
     7561 aatttatgta gaaaagaatc ttgccgaaaa tcgagttgct ctctggatta attcctaaac
     7621 tttttaaaat accgcttacc agcgggtgta gtaagggtgg aattatttgg gaaaaaattt
     7681 gagcattgcg tggcacaatt gttcggccgt aaccgccaaa agcctcgtcg ccaccatcac
     7741 cagtcaacat tccggtaatg tactggcggg cagctctgga aattaaataa gtgggaatcg
     7801 ccgaagaatc ggcaaacggt tcaccgaact gccagacgag ttccagtaga ttttcagtga
     7861 catcgtgatc taaaatcagt tcggtgtgtt cggtatcgta taatttggcg acttcccgcg
     7921 cggcaagaat gtcttccatc gggtaattct taaaacccat cgtaaatgtt ttgatccgtt
     7981 gggtggaatt ccggcataga atagcggtga taatgctcga atccactccg ccgctgagca
     8041 tcataccgat tggaacatcg cagaccgtgc ggcgcaatac agcctgaatc aataattgtt
     8101 cacagttctg caaaattata ggttcatcgt tcacgacttt gtgagcgtaa ttccattccc
     8161 agtaggtcgt gacgacctga ccattttccg agaatacatg ataagatccc ggctggactt
     8221 tttgaattcc ttcgaagatt gtgtgttgat caggaatggc aatatgatgc aggtagcagt
     8281 ccaaagccgt caggctgacc ggtggctgag ccggtaaaac cgcaaccacc gctttaatat
     8341 ccgatgcgaa atagacaata ccggactgtt cggtgaagta gagtggcttt ttcccaacac
     8401 gatcgcgagc caggattaat tgtttcttct gagaatcgta aatagcaatg gcgaacatgc
     8461 cggtaatttt ttggaataaa tcggttcccc actccgcgta accctgcaaa agcacctcag
     8521 tatcggaatt ggatttaaag tggtaaccga ctgcgagtaa ttcctggcgg agttcgcggt
     8581 agttataaat tgcaccgtta aaaaccgtcc agatgctacg atctgcattg gtcatcggct
     8641 gagtggcggc atccgatagg tcaatgattt taaggcggcg atgaccgaga gcgatttccg
     8701 gtagaagttc atatcccgat gagtcgggac cacgctcgat cataaagtcg cgcatccggc
     8761 agatttccgc cgggctgact tcgattttac cgtgatgata tattccgcag atgccacaca
     8821 taattaaaat tcacgatttc tttaggacaa ttgtgtaacc ggttttttct atccttctaa
     8881 aaccaactcg agttaaaaat tgcataacaa ttcggtaaac ttgattgaca attgaagagc
     8941 gaatgttaat gacgtctgcg ccaatctctg tcagatggta tcgatattgt aattctatta
     9001 tagaatattt attagaaatt ttttctcggg tagagttagg gctggatttt tcgcagaggt
     9061 atttttcaaa gattctggga gaataaggcc gaacatggga taaatcatcc cagaaatgat
     9121 accacattaa cggacttgaa aaaataagat agccactagt ttccagacag cgatcgcaat
     9181 tttttagtag tagaaagaaa tcattagaag tcaaatgctc aattacatgg ctacaatgaa
     9241 tgatatcgaa tttgtcgttg tgaaagggca agtccggtaa gcggctgtat tgataattcg
     9301 gaaaacgttt ttggtttctt gtaacagtct caaggttacc atcaatcatg aaaatgtcgt
     9361 ttcttccgat aaattcagcg aaggagccat ttcctgcccc aatatcgagg attattgaat
     9421 ctggtttgac gtactttttc actatctcgt aaaatggttc gcgtcgctga gaggcatact
     9481 ttaatatcct tgagattgat tctttcataa tgctttttag gtttgattta gggtcgcttt
     9541 atagattttt attaattttt ctatatagtg attccatgta taatgacttg aaatcaaatt
     9601 ccgagcattt ttaccgactg tccgtgccag atcaggctta ctaaccactt cctcaatttt
     9661 agcggcgaaa tcttctggcg aagccgtttt tgacataaat ccggttactc cgtcttcgat
     9721 aatttctatc atttgtggtg ttgaaattac cggtttacca gccgccatat attcgataac
     9781 ttttatcgaa gaaccaaaat aggtcggttc acccggataa aaaacgagac aataatcaac
     9841 cgcctgaatg atttttaaca gttcggcatg agggacaacc cctatcagaa gaactgaatc
     9901 ggtaagggat tttttctgga tatactctct gatcgtgttt tcctcatcgc ctgatccaac
     9961 gatgattaat ttcaaatccg gtcgtttttg cagggcaaga tgaaaagcct taaccagatc
    10021 gagagtaccg taccagcgat atagactgcc gggataaata aacaacggcc ggtcttcggc
    10081 gccaatttga cggcgaatag tcgttttttt tgcctcagaa agtgggtcta gcagatctgg
    10141 accaaataga ataacactat gcttaggtaa tatccaccca cgtttttggt aaataaaact
    10201 ggcgatcagt ttcgaaacta aaattagatg atcacaattt gaaaagagat atttttgatt
    10261 aagcagagcc aaccagcgtc tggatggttt aagtgggttt agttgtagag tcttctcttc
    10321 aatacctggt ccgaccatct gaagaaccaa cgggattcta agaaacttag ccgccagaac
    10381 tccaccccag gacaaaccgg tatagcgttc gtggattaaa gtaaaatgtt ttgttcgatt
    10441 taacgcaatg attttcaaga aaatccgcag gctgtcaacg attccagtgt aaggaataaa
    10501 gcgatgaaca aatccgcgta gataatgatg gggcagaatg acagtttcaa attcctcata
    10561 ttcctttagc gatttagtat tttgaaccgc gatcagagta ggatttacac ctgctgactt
    10621 tagaccacgt aatatggcct gaatgtggac ttttggtccg agcattttat caagatctag
    10681 gccgggattg gcgcatagat aaacgatgtt agtccctgga gtcataatta ctggttgcct
    10741 ctcagtttct caaatacatt acgcggtgta attccaatga tactgattga agttcttacg
    10801 aaatcaataa aaagacgtgg gtaaaaaaga cgcagactca gcgcatacaa actacctgca
    10861 aataataaat agaaaagcag gatcaggaca atcggcaacc ccaatccgat aagcaggtgt
    10921 ttgaaaaacc aaccacagat tagaaagatg acacagcaga gaaaacccgg tgagatcgtt
    10981 ctaataaatt cggtgatttt catcttcaac atttttgata cgacaattat ggtgataaga
    11041 ccgccgattg acaatacgat tgttacacca accgccgcgc cgataatttc cgccttccga
    11101 atgcccagcc agactgccac aaccagaaat ggaaattgag cgagattgat gaaaaacatg
    11161 tagtgtgttt tcttctgaat caggaaaatc cgtccagccg gcgaaaagag cgaacgcata
    11221 atcccataaa ttacgatgac ttgaagtggt cgtactgccg gaatccattt tgttccgaaa
    11281 attacgacga gcagttcata agtaatacca accagaactc cgaaaatggg gaaagtaacc
    11341 agcgagacca tttcggtcat acgcagaaat aagcgtcgca tctctgccag atctgactgg
    11401 actttggcta aagttggaag cgataccgta ttgattacct ttacaatatt ctcaacaggc
    11461 cagttggcga gattaaaagc gaacgagtaa tacccgagtt tgtcctttga aagtagcttc
    11521 cctatcagga agtagtcggt attggtgttc aaatagcgga taagctcagc tccaagaatg
    11581 cttttgccaa aagataatac ctgtttgata tcggcttttt cgatataaag tgatggccgc
    11641 catttggtct ttatccaata tggaatggct ctgagcggac tagccaaaat ttgtggaatg
    11701 accaaactcc agacaccaca accgaaaatt gccagcagaa ttaccagtaa ctgtgtgaaa
    11761 atttcgatac ttagattgat tattgtcaaa tatttataat tcagatcacg attaagaatc
    11821 gttcgcggca ccagttcgaa agactggaaa atcagtccca gtcccatcca gattaggatt
    11881 ggttggagca ccggagtatc gtagaatttt tttacccagg gtgaaatgcc aataaccaat
    11941 aagccaagta ccagcataat gccaaaattt atccagaaag caacgttggc aatctgttta
    12001 ggtcgctttt cttcgacaat gatggaggca tctattcccc aggcactcaa cagcatgaca
    12061 aatcccagaa ccaaccgcgc cagcgcaatg acaccgaaat cgtccggcat aagtaagcgc
    12121 gctagaacaa ttgaggaggc tatcgagaga agacgataac caacttgccc gataccgacc
    12181 caacggacgc cggacttagc ctgctcgatg acggaatcag ccacgtttta ctccggaagg
    12241 attccattca ccgccaagag cgcaaagatg gatatattta cctaaataag aaaacatatt
    12301 aagtatttat tcttggcgcc ctttgcggtt taatgatttc tggttattaa gaaaatatcc
    12361 aagggcaaaa tatatcagcg gaataagcgg tagaaagtag cgttggattg catgtgtcgc
    12421 agaatgcaaa atagtcaagt aaacgaccag acagattaac acaaaagaag cgggaaaatc
    12481 gcgccgagca aattgccata atccgcaaat gccgagaatc gttataaggt atctaaataa
    12541 taaagcaagt acgaaaatta ctggtgatat tgcttttaaa gtcgtcgagc cgatgggtgg
    12601 cgaaaaccag aagattaaaa atcggttaac acataaaatg caatacctga taggatgtct
    12661 aagtataatt tcaatggctt tttgttccag aaactgattt aattcaacta aagaattaat
    12721 atcgtatttt tgtaggtatt ccgaacgtag ttttgactgt aattcggctg atgctgtacg
    12781 ttgctgttca tatttagcga tgtcggtaac ggagtaccat acggttcggt tttcgatttc
    12841 gaggttagct tgccagagtg ctaatccgct ggaactttcg gttggaatcc actcattgaa
    12901 ggtaatccga ttacgaatag tccagggcac cagggtaaga attgcgataa ttgcggtaat
    12961 aagcgcatct ttcatttttt tattatgaaa gaaaatgcgg atcaataaac ttactaccaa
    13021 aataaaaatt gggagatata gaaaggttgg tcggattaga gccaataccc caattaatat
    13081 gccttgtaat atgatattcc agtttttgtt tttgaacagt tcttgattta tagttaaata
    13141 gattaataat gacataataa acattgtcaa tatttctgca taaaatgtat taacataaat
    13201 gaatgatggg ttaaaagtaa aaagtaaaat ggcaatgatc cggattgtcg cattggcacc
    13261 tatttgcttt agtgctaaat atacaatcca gattgtcaat atattgataa gtgcttgaac
    13321 tgtgtataaa tggttcggat tagttccgac aattttcata aaaaatgcta aaaagaaagg
    13381 gtagccgggc gagcgtctaa tggtagggtt ctcgccgtca atcgaaaaac gatggtgttc
    13441 gttcaaattt ttggcaatgg tataatattc taaagcatct cggtctatag gttcttcaat
    13501 tgtgataatg accgccagcc ggataaccaa accaattaaa aggatcgcaa atataattgg
    13561 gatttcatat ttatcgagta gattttgtat gcgtgaaaaa tatttcattg taattaatgt
    13621 gaggataatt atgataaact tattttaatg gagtcgcaat aatcaattgg ttaagcccca
    13681 ttagaaatct ttcataagaa agtagtttat atccagactg ttttagcaga aatttaagat
    13741 cttgttgtcc gaagaaattt tcatgttcct tagcgcctga aagactgcag agatataatt
    13801 tggcaagcga atcgtgcaat tttctcccta ttggatgagg agtcgtaata atcaataaac
    13861 catttggttt taataacaat agactttgtt tcagtaaata ttggggattt ttaatatgtt
    13921 cgatgaaagc tagcatagtc acaacatcaa ctttaggttc aggaagccat tcctttatag
    13981 tttcaaacga ttctggctga agaatatcaa gttggaggaa ttcattaaaa aggtttagtg
    14041 acggaggtat tattcggtca ataccaaaat atttacattg acttggcagg aattggcgca
    14101 aatatccggc gccacaagct aggtcaagaa ctgtggagtt attttgaata cattttgctg
    14161 cctttttcag gcgaattttc ttgataaggg gggaaagtaa gccttctgct tcctgatttt
    14221 tcatttataa tgtcgagttt ctgacgagat gattagctta tatagtgttg attaagtatt
    14281 attattcaaa ttacttcagt gaatctttct taatgcggta aagagtttct tcctgcaatt
    14341 tgcgattcgc ggcgatgaca tcagctagca agccaatcat tagcaagaaa aaaccgataa
    14401 tgagaaagac ggcggcgatg atcaccgact ggatatggcc tttgccctgg ccaacaatca
    14461 taaaaattaa ataacggaga gaaagaatta aaccaatgcc gaaactcgtg attcctgtat
    14521 agaaaaagac tcttagaggc tggtacatag tataaattct tacgattgta gcaaaggaac
    14581 gccgaatata gtaccaatta cttttgaata ggcgcgaggg gcgttcggtt ttattggttt
    14641 cgataggtac actaattatc gggatgtctt tttgtcctgc ttcgataatg gtttccagcg
    14701 tatatgagaa agtagagatg atatttatgt gcaatgcggc ttcacggcta taggcgcgaa
    14761 aaccactagt tacgtctgga atattagttt gggagacttt gcggactatg taactaccga
    14821 atttctcgag ctttttttta agccaggaaa aatcctcaat tttgtcaatc tgacggtcgc
    14881 cgattacgat ctcggctttt ttagcgagaa tcggctgaac aagccgcaca atatctgaac
    14941 ctttgtactg gttatcggca tcggtattga cgatgatgtc ggcatcgagt tccagtgctt
    15001 tttccagccc ggcttcgaaa gcccgcgcga gtcctttgtt ctgattaaaa tgaacgaaat
    15061 ggtttactcc ccactggcgc gcaatttcga ccgtgcgatc ttgacagccg tcgtcaataa
    15121 tcagaatctc gattgaatca atcccgggaa tggatcgggg aagatcattg agggtctttt
    15181 caatcgtgtc ttcttcgtta tagcatggaa tctgaattat caagttcatt tcgacctccc
    15241 cggtttaatg gcacagcccc gtcaactaca tttaagtgac ggagatttta tcgccattaa
    15301 tatcagtatc ttttgtgaaa aagtcactaa aaaacttcaa tcgttagttt tcagtttcct
    15361 tttaaaaaat tgcttaatgg cagaactatt ttaaaaacgt cttctttgct ggcgaatttt
    15421 tcaggaaaca ttttcataac gatctcagca atctcgtccc aactacgttt tccgtcaatt
    15481 tgattcagga taaaggtttg aatcagtctt ttttcgttca aactgggagt agcatcgcgc
    15541 cgcatttggt tgaattgttg gagatcaaaa aaccgaccgc tgaaagtatc ttgttcttga
    15601 ctaccggaat gatgcgtgat tttccatttg taatctatta aaccgctcgg tgatgattcc
    15661 agtaccatca gggaaatttt aaccgactcg ccggatttga cattaatagg ataacggaag
    15721 ggaaatatca gctgacccca agaattattg ccggattggg gagaattaga gaaaaattgt
    15781 tggtcggtga accagcaatc ccaccagccg attaagccgt gaatttggcc atcttcctga
    15841 gtatctaatt cgaattgttt ttggaatgta aaattggttt cgtttttcaa atctattgtt
    15901 ttgacattaa ggggtatgga tagaagcttc ttgggatggg aatcggcata ataagtctga
    15961 ttaaaggcta tttcggtggt ataactccaa tcgaaaccaa acagttggtc attatctgcg
    16021 gcaaaaaggt caagctcgtc gcgccaggct tgacattcga aaggagcgat gtataaaatg
    16081 attcgggcag gtaggaaacg accgtctgtt ttcaaaaaac gttcacgggc atcgcgtagg
    16141 acttcgagga gattgtgata gtaaaaaaat ggtccaaaat cctctataat aatcaggtcg
    16201 gctttttccg gcaaatctat ttcagttgat cggccgtgaa taaattttag gcgatcagtt
    16261 aaattgttgc gttcagcgag ttcgcgacca acatagaaaa tgtcattcat ttcgatggca
    16321 tatacggctt ttgctccggc ttttgcggca aaaaatgaat aagtaccgag agcacttcca
    16381 atttctacta caatagaatt atgatttaca gtggattcta tagcttgccg gaaattttca
    16441 atgcgaatcc ggtcactgag taaatcgcga aaatacttga tcatttcatt caatttggtt
    16501 taattgttca acgagtgctc ggctatcgga cggtgtcaaa tctatcgggc tggaaatttt
    16561 caggcgataa acggcgctca tgtccagtaa attgaccaaa aagtcgagcc atttttcacc
    16621 gctaaagttg cgattgagaa gatgttgggt taagagccga aaagccttgt gatgctccag
    16681 cggttgcagg tcaaaaccag cgcggtcggc tttttccaga aagactagtc cctttaatgg
    16741 aattttactt aaataaactg ttccgaaaag agacggcgag atatagtggg cgtcaatttt
    16801 tccgtcggat tcatcgtcga gtttgaaaat aggggaatcg agtgggatat taattttatc
    16861 acgcaaatgc ggcttgataa tcaaattgcg ggggcagggg attatttgcc ggtcgcaatc
    16921 aacaagggca aattcatccg ataagaactc ccagccatag cgcataagca ggatcgcaag
    16981 gctggtcttg ccgctggaag ccgcacctaa aaagataaag ccggtatcaa ggtgcttgag
    17041 agccgcgccg tgaaatttaa gggcggattt atttttagtg agaaggtctt gaactatttg
    17101 ccattccaga gcatagataa ttcgagataa ttggcggttg cggtgggtca ggcgattatt
    17161 gcgaaagaga aaatatttgc cgttcttggt ctcaatccga tagtggattt tagcggaaat
    17221 ttgggaaggt gttatgattg ccgaaaaaac agaagaggta acttcactaa tggttgattg
    17281 gcaacattcg agactgatct cagcggagcc aacctgaatt ttaaatagtg gcatcagtca
    17341 ttagttaata aattaagagt cctaaatttt tcgataattt tgatcacttc gctggtggga
    17401 tcagtttcaa attgattata gttctgcttt atcgaagtga ttatttcagc aacggtatgt
    17461 tgtccatcgc ataggaccca gatgtaagcc gcagttgcat tcagtgaatg gagagtatcg
    17521 gtacgatatt catacacaac tccgccgtct tttaattccc gaaatggtaa atctggtcgg
    17581 gtggtaggct taaaatccaa gtcaattttt cccatgataa ctatctattt tccaaattta
    17641 tggttgtagg acttttttcg atttccggta atttttctgc ttctaattct tccacttcgg
    17701 gaactgccat aaccagacca cacgccaacc agaagggttc gattatacgc acgatgatga
    17761 aagtattggc agtgatgcta tgagccatca ttccaatcat accgacaaaa aagccttggg
    17821 ctaacgctcg gtatagcgga gtcttggcat cggaatagac tttacgaata ttagataata
    17881 gtgaaaactg caaatagaca aaagccgcta aaccgattat tcctgtttca atgagtacac
    17941 gaatatattg agcatccaga aaacgccagc cggtaatccc aaaaccgagc accggacgtt
    18001 tggtaatatc tctaaaagcc gcccgccagc tgcgaattcg ctcggacgcg gaagtatcaa
    18061 tcgttaaacc gccgatccgt tcctggagag tagcgtcgta tccctgttgt tcggtgaatg
    18121 tatatttgac acggtcaatt acagactggg gaaaaatata aggagctaat acgaatacta
    18181 aaaccacaac tccaaaaata atccagcgct ttttactgat cgcaatataa gcgatgataa
    18241 cagctagagc ggcaagccag gagccccgcg aataggtcat taaaagtggg taacaagaaa
    18301 ttaccgttat taaaagaagc ccacgacgga gatgcctgtt ggctatagca tcgtcattca
    18361 acactaaacc taggttaata gctattagta aaacgagata accgcccagg gtattcggtt
    18421 cacctgattc accttcgaag ggagcggtaa tccgttggcc gctaggagct tggatcaaag
    18481 cgaaaattgt aactatggta aaggtgatta tcagagaaat caggtaatta ttgatttgtt
    18541 ttttggtgcg aatttgattc atcaccataa aataaatcat aaaatactgg atatatttta
    18601 aaacgaagag aagaccggta aggaaattta ctcttccagc catcatcccg attcctgttg
    18661 acaggataca cactaaagta taaatccaga ttggtttatt tagaggtgtt ttgcgaaaaa
    18721 gacccagttc tttattaatg gcagtttttg ctaaccaggt gagaatgatg attgccagaa
    18781 tcaggtcgtc aattctgata gtcatccctt caccagaagt tgtacgcgag ccaatttccg
    18841 gtgataatag catcgaaaag attaaaatat aaatcgcgaa atcggtattg gtaaaagtga
    18901 caattaatat cccgagacca actgccgcta gaatagcata aactggatca acttttacta
    18961 agacgagagc aacaagcatt gaaagaggta tacttagaaa tataaataac cggtttatcc
    19021 gattttcgaa ggcctcaatc aggctcatac taattaatgc ctttttcgct cgcttttaac
    19081 tcgcgttcga gttctttaat ctcgttctta tcagtgttct gcattatttt ttcgtaagtc
    19141 ttttgccaag tctcaaagta atctttacgc cattcagtct cgtacttttg tcgaatggga
    19201 gtatctactc gtacggcact gcccatgtac ttttcttgat gagcagtctc atcctgacgt
    19261 aaaatgctga ggaatggcca atttggttca cgaattatta taatgattgt tatagcgaca
    19321 attagtgaaa ttggaataac cagaaaaata attgcctttc tattacttct ggatttatgg
    19381 tgtttatgat ggggcatttt ttctcttttt agcgtataac gatagttttg tggcctaatt
    19441 ccgggtaggt attcattacc aattcggtgg attcagtgct agggaaacca ccaaaaagga
    19501 ggtaataggt ctttttggcg tccggttttt gactggatgg ttgtatatat aggtaatctt
    19561 taattgatgc gaaacgttct atagcttgct gggcagattg aagagtgggg aaatcatcga
    19621 gcaatatact gaatggtaat tttattgctc tgaaagtacc acggaatccg aggaattgaa
    19681 gttctttaga tttgttaatt gcttcattct cagatgaaaa actgccatag cacagtaagt
    19741 aatttttttg tttaggatcg ctaaaatcta tagtaataaa agctagaagc ccgttccgac
    19801 gaagcttatt taattgatta atcagagttg acttatcgaa agattgttca attattaaag
    19861 tataaggaaa agaacttggt aatggctgaa ctgagacggc cggagcagtt tttgatgaag
    19921 tagttgatga atagacaatg ctggcaagcg gctctgtttg cttgtcagat tgggtagtcg
    19981 caaatgaagt ggattgaacc ggtgttggag aattattagt tggtcgtcca ctttcagtta
    20041 aattttggtc aacctgttgt tgagaaatgg tctcgatacc atattgttct tggagttgtt
    20101 ttaattctgg cggaattatg gggacagctt gggcggtgag attcggtaga gtgtcagagg
    20161 ataaaatatg actggccact gaggtgtcgc tggatgaagt tgtctgcggg atgatagttt
    20221 cggttatttt gctttctacc tgggattttg cttttttccc ctgatatttg gtaaatgccc
    20281 atagaccacc gccaatgatt aaaccgagcg caataagagc tagaccagcc ctcaatttat
    20341 ttaattgact gaaaaacccg ttggtcattc ctacggctgg tttcaagaaa aaagattcta
    20401 atgcagtaat tactttgttt ttcggtttgg cagattcttc ttgatagtga tattcatatc
    20461 catatttata atcggcataa tcggcactta gttcggcttt cataccatta acgactacgc
    20521 ccagaatatc agtcttaagt aattctagtt ggcttttgga acgtcgtaga gtgccgcgcg
    20581 aaatttttcc ctgataatag actagcaaca ctgtatcaac tttagttcca aggattgtgg
    20641 catcagtagc tggaataagg ggcgaagtat cgaatattac cagatcataa cgccttttta
    20701 attcagctat taaattgtcc attaacttag aggcaacgat ttccgctgga ttgcgctcta
    20761 tttttccact ggttaaaata ttcagatact caattccggc aatgttatca gtccgaagta
    20821 tatcgcctgc catagttcca accattaaat ccgacatagt gcgaattaca ttgtctatcg
    20881 gttctttccg caaaattaca tcggttaagc ctggttcgcg atcaattccg aatagtttcg
    20941 agatacgtgg tttgcgcaga tcggcgtcaa ctaaaagagt attgatcccg atttgggcca
    21001 tagaaatagc cagattggcg gctatactgg ttttcccttc attataaatt gacgacgtaa
    21061 tcatcaaagt cttataaccc ttatccaata aagcaaaatg cacattggta cgcaagccgc
    21121 ggaagctttc ggcaatgctc gacttgggac tataatagga aacaagcctg gcttgtatat
    21181 caaggatttc atcaggaact ggagtttcgg ctttttcagc tagttgggac tttaagcctt
    21241 gaaaatcaac gaagggaata attcccataa tgttggtgtt aagggcttct ttaatatcgt
    21301 caacggtatt gaaggtcgta tccagtgtct cggctacgaa tgccaacacg atgccgagaa
    21361 ttaaaccgat gatcataccg atagctgtgg ttgggccgac tgtggtctga ttgataggtg
    21421 aagtcggcac aaaagccggt cgaatgataa acacttcctt gacttgttcc gaatatctaa
    21481 ttatggcttc ctggtagcgg atttcgagct gctgataaat ctgggtattg acatcgcgtt
    21541 cgcgctcgag gttagtcaat gtcaattcaa ttaatggtaa agacttatat tcttcgttta
    21601 atcttttata tttttcttcg gcgatagttt cggctcgggc taagttttga cgaaacgact
    21661 ttaattcatt gataaatctg ttttcagtgt tttttaattg ctgttgaagc tcgataatgg
    21721 ctggatgttg aggggtgtaa tattgggagt aattggtaat ctgggaacga atttgattaa
    21781 gctgagtttg taaatcttcc aggaagcgat taggctggct gagaagcaga tttaagtttg
    21841 cgagataaat atattcgcgg ttattcccaa tttcacgtaa aatgttgtcg atttgatgga
    21901 tgtcgcctct gattttttcc aaattagttt cggcagagtt taattcagca gttaaacgtg
    21961 atgccgctcc atcgagggtt gccagtttat tttgctcacg gtaattttgg actttctgct
    22021 cggagatacg taaagcctgt tcagccatag cgcgctggtt acggacggct tccagagctt
    22081 tttgagcctg gaggtttttt tccttgaagc tctcgacttc gtaaacctga gccagggcat
    22141 tagccagatt agcggcaaga accgggtcgt aatctgttac tattatattg ataatattgg
    22201 tgtaaccttc ctgctcggtt ttcacgcgcg atttgagtct tgatactaaa tcgaggtatt
    22261 ctttgttact gcggatttgc tcagaagtca aagatgaatc aattagccct aatttctggg
    22321 cggctttttc tatcattggg tagcttttta tttcttcggc gcgagtagcg aggtcatcac
    22381 cagttcccca ggaaatactg tacatataga gaccggttaa attagtactt ttctctattt
    22441 tcaagctggc ggtagcgcgg taaagaggag taggccgatt cattatggca aaaatcaggc
    22501 taaagcaggt gagcaataca agagtaacaa taatgataat ctttcgcttc ttgattattc
    22561 gccagtaatc gcgcagattt aattcataat gtgccatagg cagttcattt ctatttggta
    22621 tatgtgatta aaaaatagat attaagtgcg gttgaaataa tgtagagaac agtggtgaat
    22681 ggttggattt tttccgctag gttgacaata aatcctcgtt tacgacttaa aactataatt
    22741 cgatccccat cttcaagata gatatttttt tcatcttttc gatcgaaaag gtattcattg
    22801 agatcaaaag taaccggttt atagttttcg cgagtaacta taattttacg tgggtcagca
    22861 ttggaggtta aaccgccaat atcagccaga aattccgcca gtcggatatt accttcaatc
    22921 ggataaattc ccggtcggtt aacttctcca aaaacgaaga cactgcgaga agtaagtttg
    22981 acaacatcta ttgtaaccac cggctcacga taatattttt ttgccagttc ggtcagggta
    23041 gcctgggctt tgtcaacagt caaacctccc aattgaacca gaccaaagcg ccgatgataa
    23101 atattaccgt cctggtcaat tatataattt tccggggttg ccggactttt ttccatagtt
    23161 gctaattcat tgatagtgat ttccagaacg tttccagcct gcagaatata tcccttagaa
    23221 aggcgagcgt actcgtcttt taatagaagt cgtggaacga tgattttatc ctcatcatgt
    23281 aattcaacat tattggtttc atcgttactg aataagtatc gttcaagatc gaaaatgatg
    23341 acagaaccat ctttacgggt taaggtaatt ttgctgatgt tggcatcgct ggttaagcca
    23401 ccatgcgtaa ttataaattc ggcgagagtt gttccaggcg atatacgata tacacctata
    23461 ttgtcaaccc cgccccagag aaaaaccttg atcgtagttt tttcaactat actgactact
    23521 acctgcggtt gggtaaagaa ttccgaaaat tttaccgtca ataaatcttc gacctgttta
    23581 atggtcaggt tctgtacata aacgacttta agcaattcat gaaaaatagt gccgtcgtgc
    23641 cgcacctcga gaatcgaact tttttcgata ggaacacctt ggggcccaat atcaatgtaa
    23701 gagactttta ttcgatcgcc gggtgacagg gtcatttcat tttggtagga atcggtggtg
    23761 atggacggtt cacgtttttg agcaaaaccg aagaggctgg atatacaaac cagtaaaata
    23821 attttttggg gcaattttaa aaattgattt cgtttcattt tttacccgat tttataaaaa
    23881 atgctgagaa taagcattac ctctcagatt tctagagtac tggagcgtcc gtattgtagc
    23941 gggttacaat attagctagt aagctatttg ccaagtaatc cagatcaatt ggtttacaaa
    24001 tgtaatcggc ggctccttcg cgcagagctt ctatggcgat atcacgatca gccaatccgg
    24061 ttatcatgat tacttctacg gctggctggc tggctttgat ttctttcagg aaggaaatgc
    24121 catcaccatc gggtaaacga atatccagta gaacgattct gggttcaaat tctctgagga
    24181 cttcggacgc ctgtgctttt gagaaagcca ttgcggtttt ataaccgagt tgctccagta
    24241 cttcttctaa taactcagct atttcctgct catcatctac tataagtatt ttttccataa
    24301 acctgtctca ttttaagtta atttaattat tattgtattc ttagtcaatt taattatact
    24361 ttatcaatat caatatcgaa aaaattcagt gcagcctttt gaacagattg aaaaacactt
    24421 gtttttcctt acaaacctac gataggcgat gtaacttgga ttgttccaaa tttttgagaa
    24481 tttatcctgt aaaatattac caggtgtaaa attcatcgag tgataaggac gaacagagcc
    24541 atcgggaaaa acataagccg ttgcccatag actttggcag cgatttttgt atgaagaggc
    24601 tttgaattgg aattgggtgt accattggcg aatttcatca tcttctaaat tgggatagaa
    24661 attgatgtcg gttttaaact tgcgagtttt tagttcatta cgtttggcaa ttagatattc
    24721 gggatcgatt tgcggtagtt cgtccacgat aaaaccgagc cagtcggtcg gcgtctggtc
    24781 gaatttttcc tgaaagaacg gaataaattc cataactgtc gcacgatcca ggaaaagaag
    24841 atggtgtaaa gttaatccac aagcgccaat gtcctccgcg attttaataa tttcatcaat
    24901 cgagcgataa ttcaattcgg taatggtgca atttatattc acgagcggtc tatttaagtt
    24961 gcgagttttt tttaaagtgt tcaattcttg taagccattg atggcttttt gaaaggtgcc
    25021 gggcacgccg cggattttat cgtgaatttc aggcggtcca tcaagtgaaa aaataatctc
    25081 gtccaacctc gaatccgcaa tttcacggac ccattttccg atgtaagtgc cattggtaac
    25141 gatgttgcaa cgcaatccgg ccgctttgac gatttcgacg actttcagcc aatttgggta
    25201 aagcatcggt tcgccgccga agagtgtgat gttggggttg tagtgccgaa cgtcgtcaat
    25261 caaattctgg atttcgtcga gggaaagttg ctgacgaacc acttcacttg gatattcgcg
    25321 gaaggcgcca ttggtacccc attgaccgca catagcgcat tttaaattgc atttgtaggt
    25381 tagaaaaagg gagattgttt ctggcccgag actattgcca tttagaaaat ggtacgaaaa
    25441 agacgctttt aatctatgga tgaagttatt tagtgcatag tcaggctgtt cgatagcttt
    25501 ttggaaagcc cgggcgaggt ttttgcgggg aatcattcga tcgtaatttt aaattgaaat
    25561 tgagaattgc tagttccaag aatgacggta ttttcgccct tatgaagttc agccttgaaa
    25621 ttggaaagac ggccaccgat cggaatttta ttgacaatag tacccaggct actgccgcga
    25681 tcgagaatgt agtattggcc attataaaaa ttgattgaca tatgattacg cgaaacgtta
    25741 taggggatgt tgtcaattag ggacaagtcg ttatcaataa aaacatcttc gcgacgctgt
    25801 ccactttcac ggccaatttt aaaggggaat tttcgtaaat ttaccgcatg gtttttaaga
    25861 gcctgtttgg caattggggt taagccctcg agtttaattt cggttggggc atttggctga
    25921 ggggcagttt tcgacgcttc tgtaatttgc gcctccatct ggagaaccag ttgattagct
    25981 tgtcgcaggt gttcgaaaaa tgaccgcata attgggataa cgaaaggcat tttatcgtca
    26041 agcagggcgc taaaatcctc ccgtgtaatt tcatcaacag tcaccgcagt cctggcgatg
    26101 acggtagccg aacgtggttt gtcgtcaatt aaaccaagtt cgccaaaaat ttcttgtggt
    26161 tgtaaaatag caagaatgac ttttttgtga tcggtcattt tagatacctc gactgaaccg
    26221 gcttttagga tataagcttc cgaaccgtag gtcccctcat taataataac gtcacccggt
    26281 ttaaatttca gttctctcat agttcttcct tatttcaatg gtcaaaatat attaatatta
    26341 tttaaggtat ggaataaaaa atattggtcg gttaaaatct gaaattttct tgagattgtc
    26401 caatatgtaa ttttgagtaa cctttaattt tcaattgctt acgatagagg attattggat
    26461 tgttatatcg ctgagatgcg gttgatggat ataaaaattt aatcgtcaga cttagggtta
    26521 aatggtaggt gaataatgaa ttcggttccc tgaccaggtt gacttttaac tcgaattttg
    26581 ccgccatggg cttgcacgat gcggcggaca attgctaagc ctaatccggt gcttttttca
    26641 ccgccggttg gctgaacact ggtccgactg aacggtttga agagtttagg aatttccgcg
    26701 gctggaattc cctgtccctg atcagctacc cggatttcta aatatttacc ggtagccttt
    26761 actgtcaacg taattgttgt tcccggtgaa gaaaacttga tagcattcga gacgagatta
    26821 tctaaaactt gggtgatgcg gttgggatca aattcgatcg gcggaatttg cggggcgatg
    26881 aatgttgtga agtggattga cttgaccgcc gttaaacgcg tagcgttttc agcataatta
    26941 gcaaaaaaag tactcggttc gatagttgtt ttttgaaggg tcagtttacc ggattcgatc
    27001 gcgctaatgt ccagcaagtc attgatcagg ttgagcatac gagtgcagtt attttcgatc
    27061 gttttgatga tattggtttg ttggtcagtc aaatttccga cgagcccggc cagtaatata
    27121 tctgtataac ttttaatgac tccgatggga ctgcgtaagt catgcgcggc gatgccgaga
    27181 aattttgttt cttcctcgtt taaagccgct agattctgat agaggcggct tttttcaatg
    27241 ataaccgaaa cctggtcggc gattttctga aaaagttcga tatgggcatc gcgataggta
    27301 tttttattac agctggaaaa aaatataaag ccgaccggtt ttgtctgaat aatcaacgga
    27361 caggtaagac tggagagcat tccttcggat aaaatcagac gcgtagaggt tgatcgtggt
    27421 ttttgcgcta aatattcttc aagatcgttg ataatgcgtg gctgaccggt gcgaatgatc
    27481 tgttcgaggc tactgttttt caagcgggcg gagtaacctt cttttaattt aatcactggg
    27541 gcgcgactac gagtacaaca agatcggaca tttttcccgt catcagttag tagagccact
    27601 ccgatgcggt cataagggat aatggcgtga aatgagttaa agatgcggtc aagtacttct
    27661 tccagattca agccggcgtt gatctgggcg gtcaaaccaa tgaggagaga caattcttgc
    27721 cattcttttt ggaaccggat aatctcgcga ccgagcgcgg ataattcatc atccggttca
    27781 cagttaattg gacggggagt tttaccattg cgcaggtcaa ttaatgcctg ccgatattcc
    27841 tctaatcttt tatctgtcat aaagttattt attccgtttt tgaaaatgga tattcccttt
    27901 ttgaggttac gacgtttttt tggatttctt tttgatggag ggttttggcg acgaaaattt
    27961 tttcaccggt gaaggggtcg aagccggtgt ggtacatcaa agccgaataa gtagagggtg
    28021 ccggaagaaa aatctggact tgctcgggtg tgattttcat aaaattggtc gcaaatgatt
    28081 ttaacttgac catatcttga taggtgcaac cgggatgtgc cgcgattaga taataagtca
    28141 agaattgttt tttgttcgcg gctcgggtat aattataaaa cagtttaatg aaattgatca
    28201 gtgttttatg attaggttta cccattaaat gcaatacttg cgggacaatg tgttccggtg
    28261 caattttcat ttgaccggaa gtatgttcgg agatgacttc acgtagatat tttcggcccc
    28321 attttttatc agctaagact aaatcatgcc ggattcccga ggcaacgaag actttgcgaa
    28381 cgcccggtaa gtcgcgcaat ttaataagca attcgatttg tttcttatga ctaactggta
    28441 gttgaggaca gatttcggga taaatacagc gtttttgttt acaaccgccg gtcttcaatt
    28501 tttggggaca ttcgaagccg tacatattgg cagtcggacc accaacatcg gcgacgattc
    28561 ccttgaaatt gggcattgtc gcaaaactcc ggacttcatt gatgaccgat tcgggactgc
    28621 gccattgtac cgtgcgccct tcgtggatgg caattgcgca gaaattgcat tcgccgtaac
    28681 agccgcgatg ggtggtaacg gcaaattgta ttgtttccag agctttgact gagccttgtt
    28741 tggcataata aggatgcaca tcacgctcgt aattaagatt gtaaacttca tccagttcag
    28801 cctgcgaaag cggcaatgac ggcggattat ggaccaacca gcggtcggcg tatttctgac
    28861 aaagacgccg cgctctcatg gcgtcattat ttccataaaa ttcatgaaac atacgggtga
    28921 aagttttggg attgtcacgc accttttcat aagacggtat ttccaaagta ttttccggtt
    28981 tgtgaggagc aatataacag ataccgcgaa tgtcgcgata atcggaacct ttctgaatac
    29041 tttctgccaa ttccagaatg gagcgttcac tcataccgta agtgataaaa tcagctttgg
    29101 catcgaaaag aatcgagcgg cgaatggagt cgctccagta atcataatgc gctatccgtc
    29161 tgaggctagc ttcgatgccg cccagcacga tcggtttggt ttttttaaaa tattgacgga
    29221 tgagattggt gtaaacaatt accgcccgat caggacggcg gtcgttaatt ccgccgggag
    29281 tataatcgtc atggcggcgt tttttcaaag aagcggtgta attggcaacc atcgaatcaa
    29341 tcgaaccacc ggtcacaccc cagaagagac acggttcgcc gaggcgcata atgtcgtcag
    29401 gaacattcag ggcgggctgg gcaatgacac cgactcgata accagcttta agtaaaactt
    29461 ttccaattac tgcaatgcca atgtgtgggc tatcaatgta agcatcgcca gtaaccagaa
    29521 ttacatctag ctcctgccac ccgagttctt ccatttcctg tttagtagtg ggtaaaaaca
    29581 ttaaactaaa tttccaacga ttgaactgaa aatcctattc gaaactttgc aaccacgaaa
    29641 aatgcgatat attttggata ttttttccaa gtataaataa gccacatcgc atcgaggaaa
    29701 aactggtgag attaaagcaa ttcttcctta aataacaagt tcgactcttc tgtacggata
    29761 atttttaatg atcacggagt aattaacgtc cagcagattg tcccgccagc catccggttg
    29821 agaaagcggc ttgaagatta tagccgccgg tgtcggcgtc taagtccaga atctcaccag
    29881 caaaataaag tccctgaaca attttagatt gcagagtttg actgtcaact tcattcaggg
    29941 caaccccacc agcggtaatt atcgcttcgc taaaaccgcg atgcccgctg atgtcgaagt
    30001 taaagttttt cagccagagt cggagacgac gacgttcggc cgtggtaatt tgattgcaag
    30061 ttttttcagg tggaattttt attaggtcaa ggcaaaccgg aataagtttc tgggggagga
    30121 gctcttttag gacagttttg aattgcattt taccatgctg agcaaaatca cgttgtaacc
    30181 gtaagtcgag tttttggtcg tcgagcgctg gtttcaaatc aatactaatc cgcacctttt
    30241 gtttggcatt tagggcatca accaccagtt tgctaatagt caaaattacc ggacccgaga
    30301 gtccgaagtg ggtaaataac atctcgccga attcatcggc aatttttctg ctgtcggcga
    30361 atactttaat acctacgttt tttagactca gtccctgtaa acaggcggca agattgccca
    30421 cggtgaccaa tggcaccaag gccggtcgaa ccggtgtgat ggaatgacct gcagatttag
    30481 cgagacgata gccgtcgcca gtggaaccgg tgccgggata ggatgctcct ccagtggcaa
    30541 ttattactgc gtcggtattt agtttttcag tagttgaatt tggattcctg acgattacgc
    30601 cgctgatccg gttttcggaa aggacgagct gttcggcgcg agtattcggg cgaatgataa
    30661 cgccggtagc gtttgcccaa ttgactaaag ccgtaacgat ttcattagcg tcattgcgga
    30721 ctgggaaaat acggccgccg cgctccgtaa cggttggaat tcccaactgc ttgagaaatt
    30781 ccagcagttc aggcacgaaa aaacggtgaa aggcgccgcg caggaatcgg ctgtttcgtc
    30841 caaaatgggt caggaattcg cttagctcgg tagcattggt caagttacag cgtcctttgc
    30901 cggtgatggc aagtttgcga ccgggacgtt ccattttttc aattaaaaaa acctgcgcac
    30961 ctgataatga ggcttgtccc gccgccatca aaccggctgg tccaccacct acgacgatga
    31021 cttttgggat ttttttacta tcagaattgg tccggctcat tacggtgaaa aattaagacc
    31081 gcggaaattt taaagccaat acattccaaa taaaattagc gaagtgcata aaaattaggc
    31141 aaaatggatt attagataac caatcgctaa gaatgcagag gaattcaatt cgcttaaggg
    31201 tttttaggaa atcaagatga atatgcgata tttatgctct gaaattgtgg cacaaaggtg
    31261 taagaagttg ctccgagaaa caagttatct tttaagtact tctaagctct tttgaggact
    31321 tagtagcatt taaaagcact tataaggact taacaacact ttctaaattt ctcgtcgaac
    31381 tttaataatg tatttaatca tacatccatg caaataacca atacattctc ttgtttagta
    31441 tttgaaacaa taaaaaaaaa gattagttta atctaccagt tttttaaaaa taaattaaaa
    31501 aggggataag atatgaaacg aaaaattggt atagtaatag gcggaatttt actggttgcc
    31561 cttggattta ccattgccag catcattatt agacctggca aggttgtatt ttatgaagac
    31621 aagaattctg gaatgacgaa gtttcggccg gcggcggtaa cggctggtga tgatattcct
    31681 tctctgcaag cttttaatga tgcttttgtg cagattgcgg aatcagttac tccgtcagtg
    31741 gtgaccatta ccaccgaaaa agtcatcaaa actaatctgc gtcgggattt tccgggtttt
    31801 ccggatgatg atttattttg gcgttttttt ggatttccac aaggcgagat gcggagtaaa
    31861 gcacttggct cgggagtaat cgttgatcaa aagggttata ttttgaccaa caatcacgta
    31921 gttgaacaag gcgaaaaaat cagtgttaaa ttaattgata atcgtgaatt taaagctgaa
    31981 atcgttggca cagatcctct caccgatctg gctgtcataa agattgacgc taaggattta
    32041 caaccggcga ttctcgggga ttcggatgct ctacgcgtcg gcgagtgggt tctcgcaatc
    32101 ggatcacctt tcagcgagaa cctggctcat acggtgacgg ctggaatcgt cagtgccaaa
    32161 ggtcgtagcg gagtgattaa taacaccaat tatgaatctt ttattcagac tgatgcggct
    32221 attaatcccg gtaatagtgg aggtgctttg gtcaatattc gggctgaatt gattggcatt
    32281 aataccgcta ttgctacggc gggaatgtca caaggtaatg tgggaattgg atttgccata
    32341 ccgattaatc tggccaagaa ggttatgacc gatttgatag aaaaaggcaa ggtcgttcga
    32401 gcctggctag gagtggtgat cggagatgtg gatgaaaata ttgccaaatc gctcggtatc
    32461 gaaagtcgtc agggggctat catcagcgaa gttgtcaaag acagtccggc ggagaaagcc
    32521 ggtttgaaag tcggcgatgt gattaaggaa ttcgatggaa aaacaattaa ggattcggcg
    32581 cacttgcgga cgctggtttc cacttccgaa gttggtaagc ccaaaaccct agtagttctg
    32641 cgtggtaaaa aagagttaaa aattgatgtc aaactcggcg aattacctga atctgtcgag
    32701 cgcggtgttt acccagatac aaaatctgat tccaaactcg gtatccgcgt cgacgagatc
    32761 aattcagtaa ccgccagcca atacggtatc agtcctgatg aaaccggagt cgtggttact
    32821 ggggttgacc ggaatggtag agccgctgga attttgcagg ttggtgatat tattaaaagg
    32881 attggggaac gggatataag caacgtggct gagtatcaga aagccctcga agaggtgaaa
    32941 acagacatcg ttctgattct gattaaacgt aagaatgtta ccttttttga gactatagac
    33001 cttagtaaat aactctccaa ttttctctct ccgaaaagaa gggtttccct tgcggaagcc
    33061 cttctttttt caaataggaa gtgagccatt ttaattgttt ttttagccag ttaaatctaa
    33121 atttcgcggt taattggaaa gtgctagcgg taaaccagat caggaatgga gattggtagc
    33181 aattaaatga atcaagtagg caaattgtta ataatattcg gagtggtgtt gattgtagtc
    33241 ggagttcttt tgacagttgc cggaaaattt ccatttcttg ataagattgg tcgtttgccg
    33301 ggcgatatta taattcgccg ggaacatttt actttttatt ttccgtgggt tacctgtctg
    33361 ctgattagtc taatattttt cattttgttc aatttgtttc ggcgttagga ggtttatttt
    33421 cagtgataaa gaaagtcccg tccagtgtag attttgtagc tatcgagcat gaaatcctga
    33481 agttttggga acagaatcaa atatttaaaa aattagtggc gcagaatact aatggtccga
    33541 aatggtcatt tattgatgga ccgataactg ccaataatcc gatgggtgtt catcatgcct
    33601 gggggcgcac ttacaaagac atttttcatc gttataaagc gatgaatggc ttccgcactc
    33661 gctatcaaaa cggttttgat tgtcagggtt tatgggtcga ggtcgaagtc gaaaaagaac
    33721 ttggttttaa atcaaaacgt gatattgaga aatatggcat tgagaaattc gttgagcgct
    33781 gtaaagagcg agtgcgaaaa tactcgaaaa tccagaccga gcagtcaatt cggctgggtt
    33841 actggatgga ctgggagaat tcctattata caatgtccga cgagaacaat tatacgatct
    33901 ggctttttct gaaaaaatgc catgaaaatg gttggattta taaagggcac gacgttatgc
    33961 cctggtgcac aagttgcggt gcggcgctat cggagcatga aatcgctacg gaaggttaca
    34021 aggaaataac acatccgagt attacggtca aatttccgct aatcaatcgt cctggcgaat
    34081 cgcttttagt ctggacgact acgccctgga cgttgacctc gaataccgcc gccgccgttc
    34141 atccggattt gaattacttg aaaattcggc agggcaatga aattctctat gttgtcgaag
    34201 gacgtgaaag ggatgtaatc aaaggcgaat ataccattga aggtaaaatt cccggtcggg
    34261 agatgctggg ctggatttac cacggtccgt ttgatgattt acctgctcag aagaatgtca
    34321 agcatgtcgt aattgactgg gaagaaatca gtgccaccga aggaacggga attgtgcata
    34381 ttgctcccgg atgcggtcag gaagatcacg agcttggtaa aattcacgac ctagcggttc
    34441 tggctccgct cgatgaattc ggtaattatt tagaaggctt cggctggctg actggccgca
    34501 atgtggatgg cattagcagg gatattttta aagacctgga agccaaaggt attaaatatc
    34561 gtaccgattc atatctgcac cgttacccgg tttgctggcg gcacggcacc gagctgattt
    34621 tccgattggt agatgaatgg ttcatcgcca tggatggcgt gcgtcagcca atgatggata
    34681 taactcgcaa aatccgttgg attcctgaat atggtttaca acatgaactg gactggttgc
    34741 gtaatatgca cgattggatg atttccaaga agcggtactg gggactggct ttgccgatct
    34801 atgaatgtca agattgcggt caggttaccg ttatcggtag tcgcgaggaa ttgaaagaaa
    34861 aagccatgga aggctgggat aaattcgagg ggcactcgcc gcatcgtcct tgggttgata
    34921 atgttcagat tacctgtcca cgctgtggtg ccaaagtcag tcgtattccg gatgttggaa
    34981 atccctggtt ggacgctgga atagtgccat tttctactct gcattacact actgatcggg
    35041 tttattggga agagtggttc ccggcagatt ttatctgtga atccttgccg ggtcaattcc
    35101 gaaactggtt ttattctcta ctggcgatga gcaccgtttt ggaaaatcgg gagccgttca
    35161 aaacgattct cggctatgct ttagtcaagg acgaaaaagg ccaggatatg cacaaaagtg
    35221 ccggcaatgc catctggttt gatgaagccg cggaaaaaat gggcgtggat gtaatgcgct
    35281 ggttatatgc cgatcataat ccattcacca atttaaattt cggctataat atcgcgcagg
    35341 attatcgcaa gcgcctgatt acactctgga attcctattc cttttttgcg acttatgccg
    35401 aattggatgg atattcgccc caaaatcatc aggtagctga agagaatcta agcgatctag
    35461 atcgttggtt gatggcgcgg ttggtgcagt tgattcgcct gtcacgggaa gcactggatg
    35521 attataacgt cgctcgcgtt atgaaaggcg tagatgattt tctggaggtg ctttccaatt
    35581 ggtatattcg gcggagtcgt cgccgttttt ggaaatcgga caatgaccag gataagtgga
    35641 gtgcttatca gaccttatat aaggcattga aaggattgat tttaatcctg gcgcccgttg
    35701 tgccgtttat aacggaaaaa atttaccaga atctcgttcg aggtctggag ccggatgctc
    35761 ccgaaagcat tcatttggct aagttcccgc aggctgagga ggagttcgac gaggcattac
    35821 tgaaaaaaat tgacaccatt attgaaatcg tgtcagttgg tcgggcggcg cgtaacaaag
    35881 ccaacctaaa agtgcgtcag cctttagcgc aaatgtgggt gaagttgcct gctgaggtta
    35941 ccgccaatga tctggtagat ttaacagatc aaattctgga agaattaaat gtaaaagctt
    36001 tagattttat caccgacgaa acgcagtttg ttaatattca aatcaaacca aattacgccc
    36061 agctcggagc aaaatacggt cagcgtttac cagcaattcg gcaggcaatt gccgagctga
    36121 atccgatcga agtcgcccga cgccttgaga agagtgaaaa cctcgagttg aatttaattg
    36181 atggcaaaat ttggctatca cgcgatgagg ttatcgtcga acgcaaggaa aatgagggct
    36241 tcgccatagc cgaaagtaac ggaatcactg ttatcctcga tacgaaattg accccgtctc
    36301 ttttacagga aggcttggta cgcgatttta tccggcatgt gcaaacgatg cgcaaggaag
    36361 ccgattttca ggtagaagat cgcattcagg tcaactttga agcgccagcc gattttaccg
    36421 ccgctgttca taattatatc gaatatttct gtaatgagac cttagcacaa aaagttgtag
    36481 ataattttgc gcccggcgag tatgaaaaag agctaaaaat cgaaaataac aaagttcgtg
    36541 tttctttgac acgaattaag taaatttcga gcagtaggag aaaataaata tggaagccaa
    36601 aacgacgaaa aaagcatgga gtaaagagga tcttgaatat ttcaagaatc tcatcttaaa
    36661 gaaaagagaa gaaactctta aagccatcga acggctggaa gccactaccg ttagcgacaa
    36721 tactcgcaat gacaatacgg caatggattc tgcttatgcc tatcatatgg ctgacgtcgg
    36781 aaccgactcc caggagcgcg aaaaagcctt tctctggtta gcgcgtgaaa ataaattttt
    36841 atcgcatcta aactcggcac ttgaaagaat taagactggc gaatacggcg tttgtattga
    36901 atgtggagaa ctgattatga aagagcgact ggaggaagtt cctcacactc aacactgtgt
    36961 taaatgcaaa aataagccta agaaataaac cggttttaac ccgattgata aaaaacgatc
    37021 ctttttctga taagccaaat aaaaagggga ctttttatat tgcatactat tacgattatc
    37081 ggcgtttata attttatttc ttttaaataa aataccttcc ggatgaatct ggaaccggcc
    37141 aaaattatca aaccttgtcc cgacgaaggg gttggaaaac gcctcgattt atatctttct
    37201 gaaaaattac ctgcattttc caggacgaaa attgccggtt tgattaaaaa tgggaaaatt
    37261 ttagtcaatg gacaaatctc caagcctagc tatcatctgc aggctataga tacgattgcc
    37321 gtttttgctg aaccgcaacc aatcagctca attcagccgg aggatattcc gctggaaatt
    37381 ctttacgaag atgaatattt gctggcaatt aataaaaaac cgggtatagt ggtacatccc
    37441 ggcgccggtg ttcggcaagg gacactggtc agcgcccttc ttcattatac taatcaatta
    37501 agttcgttgg gcggcagtga gcgcccggga ctggttcatc gcctggacaa ggatacttcc
    37561 ggagtgctaa ttgttgctaa aaatgacgaa gttcattgga aaatgagtcg tctttttgcc
    37621 gaacgaaagg ttttcaagca atatcgggca attgtttatg gagttccaga attagacgag
    37681 ggtataattg acgctccgct ccgtcgctct cagcgtaatc gtcagatatt tacggttgat
    37741 gaacgaggtc gcccggcgcg aactcgtttt actgttctga aagattaccg cattatggct
    37801 tatctggcgg tggttttggc aaccggacgt actcatcaag cgcgggttca tttgcaatca
    37861 attggccatc cggtagttgg cgatccggtt tatggaggta aaccgcgcca gggatcggca
    37921 gttagcgcta aggatgtggc tttactcaaa aaagtccagt ctgaggttcg ccgtcaatta
    37981 ttgcatgcct atttagtaag attccaacac ccattttttg ataaagacat cgagataatc
    38041 gcacctttac ctgatgattt tagcagaatt ttgaatatat tagaagtggg aatcgagccg
    38101 tgatcactgt tgaagaaatg acgataaatt ttttgcgatt cctagcgcaa gagcgtggat
    38161 attctgaaaa tactattatg gcttatcgtc gcgacctaga acaatttttt ctattttatg
    38221 ctgaatatgc caatcaggta ccaatcgatc tcaactccgt atcgaaaatg gcaattcgtc
    38281 attatctggg taaattagct gaatcaggac tcgaaatcag ttccatctct cgcaaattgg
    38341 cttcgctcaa agcctttttt aaattcgccg cccgtcaggg ttatgctgat agcaacccgg
    38401 cggctttgat tctttcaccc aaagccaaga aacgtctgcc ggtcgtttta accgaagagg
    38461 aaattgccaa agctattgaa ttgccggatg acaacacctt ggtcggcaag cgcaatcgcg
    38521 ccatacttga gttattttac agtagcggga ttcggctggg agaacttata gcgctcaaca
    38581 tcggtgactt aaatttcagt cggcagacac tgcgtgtttt cggcaaaggc gctaaagaac
    38641 gagtagttcc gtttggcaaa aaagccgctg aagcgctcaa taattatctc aatatgcgga
    38701 aaaaggaggt aggcgcattg gagttaaatt caccgctgtt tgtcagtagc cggggtggtc
    38761 ctaactctca acctaccggc taccggcgaa tttcgcgatc gcaggtgcag aagttaatca
    38821 gcggtttatt acaacaagct tccgagcaaa ctcatctcag cccgcatgtt ctccggcact
    38881 cgtttgcttc acatttattg gatcatggtg ctgatcttaa tgcagttaaa gatttactgg
    38941 ggcacagcag tttatcaacg actcaattgt acacgcatgt ccgcattagc accatgaaag
    39001 aagtttatca acaagctcat ccgcacgcgg attcaggtaa gaaataaaag tcggatgaat
    39061 agcctaccgt taaaatattt aacccaaaaa ttatccaatg ccgcatttcc ctttgattgc
    39121 ggtttgaatt ggataatcaa ttttaaatag ccaaaggagg tttcaaatga atatcgaact
    39181 aaccacgcgt cattatgaaa ttaccgatcg tgccaaaaag catcttgaag aggaaatgaa
    39241 caatcttaaa ctttacgaag acctcattac cagctgtaaa gtcattatgg agaaaactaa
    39301 ggacggcgaa tctattgaaa tctctattca tgtgcgcggc aaagacctga tcgcgcaggt
    39361 cgcaagtgat gatatgatca aatcaattga tttagccata gcaaaaattg aaaggcaatt
    39421 gaaaaaattc aaagagaaga ggtatgcgcg atgaatcaga atgatttaac agtcggacgg
    39481 atatatcatg aaaatcgcaa gaagttggga tttttacgta tcaatggtga tattggtcat
    39541 gatacggtcg taacttcatc caaattgaac cgtccaggat tggaaatttc cggcttctgg
    39601 gaaagttttg atcctcaccg aatccagttg atcggagagg gtgaatttcg ttatatcaaa
    39661 actctttcag gagacaaggt tgaagaaatt ttcgagcatt ttttttcggc taaaataccg
    39721 ctgatgatct ttgctcatga tgttaaacca ccggtttgtg ccgttaggct cgctcgtaaa
    39781 taccaggtgg ctctattagc gacgccggta acgacgacct tattggtggc ttctctcctt
    39841 gattatctgg atcgcgagct ggcgccttca acgataattc acggcagtct tgtggatgtt
    39901 tatggaatcg gcttgcttct gaccggaccg agcgggatcg gtaagagtga aattgctctc
    39961 gatctggttg ggcgtggcca ccggctggtg gctgatgatg tcgtcaaaat tattcgccgg
    40021 gcggataata tcattatcgg tagtggagta gaaattttag aacaccattt agaattacgc
    40081 ggactgggaa tcgtcgatgt ccggaatatt tatggcatcc gggcaattcg tcagcagaag
    40141 agaatcgaag tccagattga actcgaagaa tgggataata ccaaaaactt cgaaaggatt
    40201 ggtgctcaag aaaaatacaa caagattctc gatgtagata ttccgattat caatctaccg
    40261 atttatcccg gtaaaaatat tacagtaatt gccgaggtta ttgctctgaa tcatgttctg
    40321 aaaattatgg gtactaacgc cgcacttaac tttcagaaga aaattgccgc cacgattcgg
    40381 caaaaaacac aaatcagtga tttacagaat tatctgaaaa aagatttgga atagaaagga
    40441 agttggattt tgaaagctat cattcctgcg gccgggattg gcgaacgcct taggccattg
    40501 actttaaccc gatcgaaagt gctcctgcct gtggcaggca aaccgataat tggccatatt
    40561 tatgaccgcc ttgccttgac cggaattaag gacgtgacag tcataatcgg ctattacggg
    40621 gagcaagtgc gcgactactc attaaagaat tacgacttta attttacttt cattgagcag
    40681 tcagagcagt gtgggctggg acacgccgtc ggattaggct taacccctga ggatggacct
    40741 gtgatgatta ttttagggga tgttatcctc gaacttgatt ttaaagggtt gagcagtcgc
    40801 tccgaaaacc tgattggcgt tatgccggtt gatgatccac gtcggtttgg aatagtagag
    40861 atgaaagatg ggaaagtaac tcgcctggtg gagaaaccgt ctagttctac cagcaatctg
    40921 gcaattgcgg gtatctattt aattcaaaga ggcgcattgc tcaaaaaagc tattgactat
    40981 atcattaccg tcaatattaa aactaaaggc gaataccagc tgaccgatgc ccttcagaaa
    41041 atgctggaat ggggcgaatc gttcgaggta attaacatcg aacataacct cgattgtggt
    41101 acgcgtaagt ctttgcttaa gaccaatgcc tatttactca acaagaatcc gcaaccgcca
    41161 cccgctattc cagagacagt cattattcca ccggtttata tcggccgtga tgcggtaatc
    41221 aaacgctcga ttattgggcc gaatgttcat cttggggctg aagttcaggt aatgaattca
    41281 gtgttatcga atgccataat ttcggacggc gcgatattaa aggacgttat attagatgag
    41341 agcctgattg gccaaaaaag tcaggtctcc ggaatcgttc agcagattga tacggctgat
    41401 tatacggttc agaaattaag ttgattttct cgatacatta cagtattttt gaacgtttta
    41461 atttttaata atggagttag agacaatgaa aaaatattcg tttacctcag aatcggtgac
    41521 ggaaggtcat ccggataaaa tctgtgatca ggtttcggat gctatactgg atgcactttt
    41581 gacagttgat ccggagagcc gagtcgcttg cgaaacgatg acgaccactg gtttggttat
    41641 ggtcgtcgga gaaattacga ccaaagccta tgtcgatata ccgcggatag tacgcgaaac
    41701 cattgaaaaa attggttata ccgatcccga ctttggtctg gatgcacgtt cctgcgctgt
    41761 gataacatct attcatgaac agagtgaaaa tatcgccctc ggcgtgaatg aaactgagaa
    41821 acacgagcaa ggagcaggtg atcagggttt gatgttcggc tacgcctgcg acgagacgcc
    41881 ggagctaatg cctttaccga ttagtatggc gaacaagctg acgatgcgtc tggcagaagt
    41941 tcgaaaaaaa ggaatactca actgggtcag gcctgatggt aagtcccagg ttaccgtcga
    42001 atatgaagat gatcatccca aaagaattac caatgtagtg gtggcggtac atcataatcc
    42061 cgaaattgcg catgagaaga ttgttgcgga tattaaaaat catgtgattt taccagtttg
    42121 tcagggatat cttactcctg aaactaaatt ttatatcaac gcgactggca tttttgctat
    42181 cggcggtccg gaagccgact cgggcgttac cggccgtaaa ataatttgcg acacttacgg
    42241 tggtatggga cggcatggtg gcggagcatt ctccggcaag gacccatcca aagttgaccg
    42301 ctcggcttcc tatatggcac gttacattgc caagaatatc gttgcggctg gtttagcccg
    42361 ccgctgtgaa gttcagctgg cgtattctat cggcgttgct gatccgattt cggtctatat
    42421 taatacttcc ggcacaggta aacagcctga agaacgcctg gcggaagccg ttcgagcgat
    42481 tttcccctta aaaccggcgg caatcattaa atatctgtgt ctcaaacgtc cgatttatct
    42541 caaaacagcc gcttatggtc atttcggtcg cgaagatgaa gaattttctt gggagaagac
    42601 cgacaaagtc gccgagttaa agcaatattt tggagtaaag taatgactgg aaaaatcaag
    42661 gatatcacac tagcaccctc tggaaagttg aaaatagaat gggcggaatc gcatatgccg
    42721 gttatgatgg cgttaaggca gaaatatcgc gattccaagc cgctgaaagg gatgcgtatc
    42781 agcggttgct tacatgtcac caaagagaca gctgtattga ttcggaccct gatagcggct
    42841 ggggcggaag tagcctggag cggttgcaat ccgcttagta cacaagatga tgtcgccgcc
    42901 gcgctttacg atgagggcgt tccaatctgg gcttggcacg gtttgacaac cgctgaattc
    42961 tactggtgca tcgaacaaac tttgcttcaa aaacccaatc tgacgcttga tgacggtgcc
    43021 gatctggttt ttactattca taataaattt ccggaactgg cggctggagt tctgggcggt
    43081 tccgaagaaa cgaccaccgg tgttcatcgt ttgcgagcca tggctgagga tgggaaattg
    43141 ctctatccga tggtggctgt caatgatgcc gaaaccaaat gggatttcga taatgtctat
    43201 ggaaccggcc agtcctcaat tgacggaatt ttgcgcgcca cttcgatact ttatgctggt
    43261 aagacaatgg ttgtcgcggg ttatggacat tgcggaaaag gcgttgccaa acgtgccagt
    43321 ggcatgggcg ccaatgtaat tgtcacagag gttaaaccaa ctgcggctct caaagccaaa
    43381 ctggatggat ttcgagttat gccgatggat caagccgccg agctaggcga tattttcatt
    43441 actgccacgg ggatgaagga tgtcattgtc agtcgtcatt ttgaaaaaat gaaagatggc
    43501 gctatcgtct gcaacaccgg tcactacgat tgtgaaatta acctgaaaga gctagccacc
    43561 cttaccaaaa cgaaacaaga aatgcgtccc aattgcgtta agcatgaatt aaccaacggt
    43621 cgttcgatct atgttctggg tgaaggacgc ctggtgaatt tggtagcggc ggaggggcat
    43681 ccatcggaag taatggatat gtcctttgct aatcaattta tgacaatgat tttcctagct
    43741 caaaatggag ccaaattagc aaagaaagtt tacgacatca gcgtcgagca ggatcaggaa
    43801 attgcgttct taaaattaca aactatgggt gtcaacattg atactttaac cccagagcaa
    43861 gttcgatata aaaccgccta tagcgaagga acttaaacta cgcattgccg gtcgaaatgg
    43921 cttatccttt ttctgaccct cctaatctgg ctgggcaaat gtgagtttaa aattccaact
    43981 gctccgagtc ttcccgcaaa atggaattct aaaataattc tacccttaat agataaatcc
    44041 tattcatttc aggatttgct tgataccgag aacggcaatc caattaaagc cgatcaaagt
    44101 ggtgttctat attattcgtt tactgattca accagtatgc cgatcgagat caatcctacg
    44161 accttccgga ttgtaccggc tatgactatc agtcagacgc tcgacttaag aaaaaatatc
    44221 aaacgtaatg tcaacaacac ggtcaatccg attaattata taatgaaaaa agcctgggac
    44281 gattctaata accgagttac ttctggtgtt ctggacaatt cgaccggttt cactgccgac
    44341 aatattccga ttaacgcttt ggtactggcg gcacagttga gcgattcttt tcccaacccg
    44401 attcagattc gcgtaatagc tccgaatttt caaaaaaatc caggtcaatc agtagtcgct
    44461 actcttgatt tacctgttaa tagtttacag gatagtataa tgatcgaact gggtggcgct
    44521 tcgttgatcg gaggcttatt aaaagaagag attgattcgt tggaatttaa tatcgaagtc
    44581 caagtgctgg gcgaattagc ggaggctacc agtagtctca aacaaaaact gacgacttcg
    44641 ctgacagttg gccaattagc tctagaatca tttaacgggc gagtgtttgc gacaggcgag
    44701 acgccacccg ctctggtagc gaaaagtcta accggagcga gcgatattga attcgacagt
    44761 gccagggcga gtctgaatat tgaaactttc ggtggaaatt ttgattcggt ttatgtccag
    44821 ttgagcggca agaaagttca tggtgttgaa acggtctctg acactatgac agcaataaat
    44881 cgagcggatt taaagatgga tatcggtgag gttgtgtcca atcttcctga tactgtccgg
    44941 gtttttgtca aagttatgac tgccacggcg gattttgagc gcaatactgt ttttaatgcg
    45001 ggcgtgaacg tcaagtataa tatatcggtt ccgttgaggt ttaaaatacc gccgcagatt
    45061 acactagctt ccggtaatac aactacctat tttattaaag acagcacaac tcgcgctcgg
    45121 ttaataacgt cgcaaaatgg tgctgatctg gatatgacgg tcgaaaatcg caccgatctg
    45181 caaggtgatc tctatttttt aatcagtaat tttaattttt ttccatttga tacgcttgga
    45241 ggcaatttac caagcgattt tattcttttt gatggaactg tctgccacct tggggacgat
    45301 acaaccgtgg tagaaattga taccttggcg gtgttggaat ttccacccgc tcaatttagc
    45361 gagaatactt taatagtacc cggaatcaaa agtcaaattt ttcagataag ttctgatgcg
    45421 attggattgt tagcagatac gtgttacttt aaaccttatt ttgagttggt taatccggat
    45481 acgagtctgg cgacgattac cgcaacgcag tcaattcatg tcaggggctt tctggacttg
    45541 tactttgttg cgacggattt aaaagattct gactgaaaat gaagccgaag aaacttagta
    45601 aacgacttac ccgaacctta agatcatcaa attggttgtg gcagttgata ctgcttgggt
    45661 tttttctcta cggattgccc cgaccgctcg atgctcagta ttatgatagc ccgattaacc
    45721 gcgctctggg tggagccggt aatgcgataa cgcgcgggat tttttgtgtc ggtcagaatc
    45781 cagccaatct cgggctcact gcgcaatatc gtacttacgt ttatctttta ggaataaatg
    45841 gttatgtcac caataatttt tattcactcg aatctggggc tcgttttagt gagaaagatt
    45901 taacagccaa tggtggtgaa ctacaacgcg aatttttaga aaaattaccg gggggcggtt
    45961 atcgtcttaa ttccagcgtt gcatcgcctt tgccgttggt gaatttttcg gtaggtaata
    46021 aagcattcac aacctcgtta attcaaatct cagactacta tctttccaag ccggctctgg
    46081 atgtcatttt cggcaattgg gaaaagggaa cgacctataa cttggatttg cgatgcgatg
    46141 ctatgactgc cgtcgagtat gtttattcga tagggattcc tttcggcaaa atgtcggttg
    46201 ggatttcttt aaaatatttg caaggtctgg gatattacgg attggatccc gcttacagta
    46261 ctggccaaat cgcggtggac acgagtaact ttgtgcttta cggtaatggc tcgtattatt
    46321 ttcgcgagtc cagccatggt cgcggctatg gggcagacct aggtgtcgca tttgaagatt
    46381 taaacggctg gactctgggc ttcagcgttt tgaatctggg tgaatcggtt aaatggaata
    46441 ccgaaacggc gctctcaaaa tggctcaaag acaatggtat tgatgtttta aaaatgatgg
    46501 gatttcaaat caaaagtttt ccagtaaaaa ataccgacct gaaacttgat ttcgagggtg
    46561 agagttactt ctacaatttt cggattgaca gcttagatgc cgatgccctt ttccgcagtg
    46621 aaaatactta tcaagatcat ttcagcgata ctaaattaat taaacaggat tcaagtgcct
    46681 ttcgggttca agtgccactg gtgctgaaag tgggcgcggc aaagcaattg cgcaaagatt
    46741 tgctgactgc gattgatttt agcatgtcgt ttagcgatcg gctgaatctt aggaaagggt
    46801 ggcggacagc ggtcggtttt gaatatgcct attttcccaa aatgccatta agggttggat
    46861 tagcactggg aggtatcagc ggttgggaat ttgatatcgg tagtagtgtg aatcttgcgg
    46921 cactgcatat tgactgggcg cttggtatga atcgaggtct ttgggtgcat tcgatgcaag
    46981 gtttcgattt tgcgctagct acctatctgg tgcgtggtgg caagaagaaa taaagctggg
    47041 tactaaaatt gatctggatt ttatctttta ttaaagaact gtttttatta aagcaattta
    47101 ggctctattc gactattttt tttcttagca tcggattgat ttgcggctgt tcctatgagc
    47161 cgctggcgcc gccggaaatg ccgacttatt atcagacgat aaatctgcca ctggctgatg
    47221 tttctttgcc attagccgat ctggtcaatc ctgataataa tatttacggc gattcgacta
    47281 aagaccaact ctattttcaa tttagtggta accttgacac cgtaactctg acggaaaata
    47341 tttttcagat tcccgccgtc ggtaatgtta attttagtca aaacttttcg gaactcagcg
    47401 aatctcagtt gacttttagt agatcggtaa cccaagctac caaactatcg gaaattatca
    47461 ctattcccgg tattttacct tcgccgaatg atttgctcat aggtgagatt gcgcgctatg
    47521 aattggatgg ccagcgctat aaatatcggg tttttgacaa ggatgatatt ccttattttg
    47581 agcgggtaga ttatctgacg gtaggctccg gcaccttcca aacggaaatt gataaccaat
    47641 tgccggtaga tatggattcg gtccgaatta ccttgcgtaa taaaaacggt tcactaattg
    47701 ccgaatcttt ttatgaaaat gtcccagccg gtgcgacgct aagcgacggt actccaggta
    47761 atttaaacgg cgcccaatta tttgattcaa tcgaagtaat tgttacggcg gtaatggctg
    47821 gcagtcaggg acagccggtg acaattccgg ctaataccga tccatttatt gcgatcaaat
    47881 ttgacacgaa tattgaagaa attgaaagct ttaccggaat tcccaagccg attgtaacta
    47941 ctcaaaatca gttattgcca ccatctaata acacaatttt caaggcgaca attgcgcaga
    48001 caaccacctc tccgctcgat accaattttc ttgatttgac aattcataac ctgatgccat
    48061 tcaatctcag gatgcaggtg atttttcata atttttatat caggaatgaa cctctcacta
    48121 ttgacacgat tgttcagtct ggacaaattg tagagaatcc cgcacgcctg gatggttata
    48181 tttttcgtaa tcctgattct actacggtag tggattcgtt tttggtcgaa ataattgcaa
    48241 caattttacc gaatgcaggt gaacaggtgg ttacaatccc gaccgatatg ggtgatgcct
    48301 ctattgatgt taacctatat tttgcgcgtc tcaaattaga tatgctggaa ggatttttca
    48361 atgaatcatt tgacatacca ccaatggcga tagccaatat tccgacgggg ttagcgaata
    48421 tagatttcgg ccaagttttt ttgaagttga ctttcttcaa tgaaatacaa gctcgtacca
    48481 atgtcagttt agtactgaga gggttgaaaa aaggtgtcgc accccagacg atcacggaag
    48541 acggttcgat tgccaaagca tccccagcta atctaattca agaatcaatg atggagattg
    48601 atattgcgcc gatttttaat atggtgcctg attcgatttt ggtcagcggc caagccataa
    48661 tcccctccga cgatacttcc cgcctgcaaa tcggcaaagc cttttggggc ttttatgaga
    48721 ttactgttcc atttcaggtg aagatcgggc cagtgacttt tattcctgtc aattcaacgg
    48781 agatggcggc tattgatccc gagacccgcc agaaaatccg cgatggtctg ataaaatctt
    48841 ccatttatac tgagataatc aatgattttc cattttccgg taatttatcc atattaatgt
    48901 cgaattatga ttattttcct ctcgaaccgg acagtctgga cgcaggttat ttctggttaa
    48961 acgatacctt gtacgccaga actgacaccg gcgatgttcc gataataatt gataccttgc
    49021 tgttagtcga attgccgccg ccggaggctt ttaacagtaa tggaagtgta aaaactcccg
    49081 gctttaccct tgccgaaacg ccccttgatt cggctaaaat ggaagcaatt ttgcgagatg
    49141 aaactcatta tattcggccg cgagtgcatt ttaatgcaac cgaagatttt gtgattgttg
    49201 gttattatga tgaggtaaga attctggcgg cgatatcgct gacagttgag acgatggaac
    49261 tgattaataa caataatgaa aatgaaacgc ctgcaactgt tccgtagtac atttacgatt
    49321 tcacagctgg ttggttggcg aagaaaaaaa tgggctggtt aaacaatagg tataaaatca
    49381 ctctgttact gatgctcggg atgagcatta tggcaacggc tcagacgcga cgcgatccac
    49441 gcgctttagc actagccgga gcttatggct cgttagcccg cggtattttt gccatagatt
    49501 ataatccggc taatctggct attccccatg aataccactc atatcgtatt ttcggtggat
    49561 ttggaaccaa tgtgtcaact aacttccttt caattaaaca gtatcaaaaa tataacggta
    49621 aggatctaga agcaggcaat ggccatttaa agaaggaatt tatgaacgac attcccgatg
    49681 aaggctggcg ggtttttacc gacatatatt ttcctctacc gttgatcagt tattcgacat
    49741 ataatcgcgc gatcagctcc gacttgatcg tgattggaga cttaggcttg ccgaaggatt
    49801 tactgaattt tgtctttgac gaaaacccaa tcggagaaac tctcgacctg gatttcaacg
    49861 aggaggtaat ggctataatt cagtggggat attcactggc ttttccagtc ggtaacattt
    49921 ttctgggatt tacggctaaa tatctgcaag gtcttgccta tatcgggtta aatccggatt
    49981 cttcctatgg ctcggttact acttattttg agcctgagcg gacttatatt gacggttatg
    50041 gacagtatta ttttcagcaa tcgttaggtg ggcgcggttt tgcgctggat attggactga
    50101 cgaccgaaga attaaatggt taccgactcg gcttaagcct gactaatctg tttggatcta
    50161 taacctggaa taaaagcact ctattgtccc gatcggtttc agcggaaaaa gttttaccgt
    50221 gggagggtaa tttttacaaa tatgtgttta gtattgatga agcccggttc gataagtttt
    50281 ttggaaaagc tagtgtggac gatgtctttc agggtgaagg atataaaatt aaagatacaa
    50341 ccgcatttcg ggtgaaatat cccagtttgg tacgtttcag tgcggccaaa atactggaag
    50401 aaaatataac gctggcttct gatttagtag ttggttttga ggatcgtttg tattcatttg
    50461 gcgcctggaa gtggtgtctg ggtttagagt caactaaatc acagcgcttc ccactaagag
    50521 tagggctgtc ttttggcggg cacagttcac gggaattgac cttcggaagt ggtttccact
    50581 ggggattgat tcacctggat tgggcgctgg gttttaatca tggattatgg ccgacgacaa
    50641 ccaccggcct aaatttcaat atgctatgtt attcgaccaa aagggcaaag acttcctata
    50701 gatagttagc gcaattattg agaactataa taattactca attgtcaatt aattgctacc
    50761 ttgttgtaaa attaaactcg ttcagccatt caatcatacc cttgggcaat taaacataaa
    50821 aagcctgagg ttaaaaattt tcttcaatgt acttaaaagt gcttaaaaac tcatccactc
    50881 gtaaaattat ctattcttat ccggcaaatt atgcggttga ggaaaaatta aagtttttcc
    50941 gctactgtcg gattttataa agtaggtaac ctccggcgat caggaagact atattaccga
    51001 gccagaccgc aagaagcgga ctaaggattc ctttataacc caatacctga cctaacttga
    51061 taaatccata atagatgaat atcgccaata aactcattcc agcgccgaag gcaatcccct
    51121 tatgcggttt agccgccgcc aggggaaccc cgaaaagaat tacaataaaa ttggtgaaag
    51181 gaaaagccaa cttgaaatgt ttgttaactt cccacttacg ggcgtcgtta ccgctttctt
    51241 gaagacgctt aataaaatag gctaattcgt tatatcgcat accttccgga ttaaggtcgc
    51301 tcttgacaat atcttccggt tgtaaattaa gctgaatcaa ggtatcgctg gaggcggcaa
    51361 ccacggtttc agtaccatct tggtcgaagg agcgaacttt atagtcgaat aattgccaga
    51421 gttgttgatc gggcaaccag accattttgc gagcgtcaat gcgtcgcacc agccggaact
    51481 gatcattgta ttgaatagtc acgttctgag cgatattatc tttcgtcgaa aaattgccaa
    51541 tcacgatgtt gcaggtcggt gaatcctgat acataatgtt ggtaaagaaa gtcttgggta
    51601 tatatttgcg acggtgcata ttatgtgcct caatatcaat tcgccgacgg gtagcgggaa
    51661 ttactacggc atcttcaaag aaaaacgagc cgatgctaat taaaattccg gtgattagta
    51721 acggaatcga aacccgataa agactgatac cggaggattt gatggcgtct agctcattat
    51781 gtttcgataa cattccgatg gtgaataccg ccgctaacag taaagccatt ggaagggcaa
    51841 gattaaggta ataaggtaat tggtacaagt aaaaaagcgc tatcaaattg gacggcatat
    51901 cggctttgag gaatttatct agtttttcga ttaaatcaac aatattgaaa ataaccagaa
    51961 aagcaagcag gcaaagcccg aaaatgagta gaaatttctt gagtatatag cgatcgagta
    52021 atttcaagtc aaactccgct aaaaattatc accggcgcag atgtttatca aatttttatt
    52081 ttcagactgg gaaatcgagc acctgaaagg tcggttatta gtctttaaac caaacgatga
    52141 aagatgaaat aggaaaggaa ttgaacttaa gaccttgagt cgcaagcacc gattaagatt
    52201 attaaatggt tggtaattga atgatagcag acaagccgca ggtctatcaa accggggaaa
    52261 ctttgattac cgggatacgt aaccggcgac ttttttcatt ttgcgttcga cgcagatatt
    52321 gattgctcgg acatagccag cttcacggaa agactcttgt aaataagtga ttagtgcatc
    52381 aagagtttcg aaattactca gttcggtgga ttccagcagt cgggcaaacg aacgaaccgc
    52441 cagcaattca tgccggtaga gtgccatatt ttccaggtaa acataatcgg tgattttgcc
    52501 agcatctttt ttcttctggc attcattcat catcaattca atatcggcgg ctaagtcttc
    52561 gatttcgatt ttcagaatag cgacgaagtt ttttatgatg tttagcataa tacctcccgc
    52621 ttttaaaaat cttacgattt aatttataac caatattggg aattgcaagt gctttaatgg
    52681 gtagaggaga aaagccttag aaagcgagct tgagcgctac cgggcggtgg tcggaaattt
    52741 taaccgcata ttcattccag ccaccttcca tataattttc gatcaataat gttttaatca
    52801 cagatgtaga attggcgaaa acgccgaata attcatcggt gatgagaatg tggtcaatat
    52861 gacttggcca ggttggatat gaccagtatt tactactgcc ttgtgcaata gagtaatcgg
    52921 caaatttata ggcggaaggt tggcttaaaa atggccaaaa aacattaact gactgagcat
    52981 cggtcaattc atcatttaaa tcgccgatta cgatgacatt atcgtcagcg agatttgttc
    53041 taatatattc ttctaataaa gtgcaggctt gcagacgacg attttgtgaa tcttcatcgc
    53101 cctgtgcttt aaaatgatta ttaatgacaa taataggaac gttttgccat tccaattcta
    53161 tcaccagcgg cgggcgggga aaagcgtcct catgttcaaa gatttcataa tattgattga
    53221 cagtgatatt tgcaggatta tacacgaaag ctagattaat accgtaagcc gcgctattgg
    53281 tgcggtagcc gtcccaacta ccgagcgaat ccagtaattg ttgaaaagca gtggcgcttt
    53341 ttatttcttg catgccgatt atgtcaatgt cgagggcttt aatggcttga ctgacagcgc
    53401 ttacggtgag ggtgtcaaaa cgagggaatt cctcgatatt ccaggtcatt acctcaaagg
    53461 tcggttcagt accaaaataa aggttagcta aagtgagtga acctaattta atttttacta
    53521 tttctgtgaa agaagaaaat gatgatccca cataagcggc gatccggtag ctaatctctg
    53581 aactggtagt ggttatgtcg aaatattcat aggaattgga atcggctttg gcataagcga
    53641 tttgccaatc gtcattacca acctgccgat cgattcggaa accggcttca ccgatggatt
    53701 ggtcccgcca agcaatccgg attgtggatt tatctatttg ccgcgctgaa aaatctaccg
    53761 gtacacatcg ctccgataaa agcgctacgc ttggagaaat tgaagatgaa ttatcacttt
    53821 ctttgaaacg taaacggtag gaatagaccg tatcagtgtg aacggaatat agcgtatcta
    53881 catagataga atcattagct ggcacattgg tataatccat ttgccaagat gcttcaccga
    53941 ctttgcgatc gaggctgagc agtgcttctg attcgaactc tccttgccag gttattgcca
    54001 gggtgctgtc cgataatatt tgcagattaa tatttttgaa cggattttct ggctttggag
    54061 aaattggatt ggagcagttt gaaatactag cccaaaaaat gattagcgaa aatagctgaa
    54121 aacgatgcat tactataaat accgcgattt aaggccaaat tttataaaga taaccaccat
    54181 acatcaacaa aagaaaaaga ccctcgaatc ggttgatttt taagcctata aaagtaaatg
    54241 gataaattgc taacgagagg atagccataa aaagcaattc aaatgacttt attccaaagc
    54301 cgttgattgg agctattaat ccagctacta ccatcattaa tagcatattg aaaatgttga
    54361 aaccgatgaa attttcgaat aaaacacaga acaattaaca ttaatatctt ctgattatat
    54421 ggagatcaat atgccagtca aaaatatatt gcttatcaat tccaaataaa atgatagtcc
    54481 gcctacttct ggaatttatt gatgcttgcc ggattgataa ataacaagtt attttcattc
    54541 gatgttacag attttcggtg acccggcgcg attcctcaga atgacgataa aattcctttg
    54601 gtgtcaattc tttgataaaa acaaatccgt gggtagcgca caatcgagga tgttcatttt
    54661 tgataaaaaa atcgcgtccc agacaagtct tgatttttaa gtattgatca acctgaatct
    54721 tgcgcgccaa tcggcagaac ggtccatcga attcccagct gggaaagcta gccgatcttt
    54781 gagagccgaa gctttccata aaggcatttt ccataatagc cattgagttc aggctgaccg
    54841 gaacgtaaat attggtgcta gcgggatgaa aacggtgcca gacgttgcga tagccccaga
    54901 tgcgtagatt agagatatct tcttcttttt cataaagttt aagtgtctca gtgatggctt
    54961 gcagaacttt gtaatgcgtg tcagggccac tgccttccgg gtcaagggct accgtaatga
    55021 cggtcggttt tattcttttc attaattggt aaataggaag gacatcgcga tccaacgtcg
    55081 gttcttccga aaaaatatcg cctttataaa aacctaaacg aaggtgaaag acattattgc
    55141 tattaaaacc aaaatagccc cagagaacat cagcttccca ttctcgaatc attcccttta
    55201 atttctgaat gtgtgcgatg tcttttttac cggggtattg ggttgtgcaa taattgatta
    55261 attcgctaat gcggtctttg aggtagatga cgttattttc ttcatagatt tcagtcaaaa
    55321 tggataatag tcggcgcgat tcggcttcag tttttttctt agcatcataa agggcaaccc
    55381 cgtcgagata caaattgacg tcccggttac gggcggcggt gttggcgggg tcgaaatagt
    55441 gttcctgcat taaaacctgg aaagtagggc tatctaccaa atcttgtaga tttttcaatc
    55501 gttccagtac ataggaatta gtcacggcgg tgaatccact ggtcatatag gtgaaataat
    55561 gtttgttacg tggatttctc acgagatgaa caatataagg gaggtagccg agcatttcat
    55621 cgtcgtgatg tggtgaagtg tgcataaaga cttcgttgtc taaataataa atgccacgat
    55681 ctatccggtt gataatagac tcgtgtaaat ttctacagat ttcggtatgg gtctgattcg
    55741 tacgcttaag aatcaattcg gtaaatcggt tagagttaaa atccgcgagg gttaagtcgg
    55801 taagtttttt atttttttcg agagtgagat taatgactgc tttctcgatg gttttcggct
    55861 ccaattcggg cgactttgcc attttttcat atcgtctttc aaacagtttg ctggccgcgc
    55921 cttccgtcaa gtagaagcgg gcgttaggta atttttgcaa aacggtggct ggatactgga
    55981 ttgaaggaaa attttggata gaatcgcgca caatattggc tttagcttga ccggcggcga
    56041 tgataattgc ggtggcgtct ttattatagg taatggtatc gaggccaatc gtaattacca
    56101 ggcgtttgcg agctatttcg atgccgccta aatcgctagc cgcggcggct tgggtttcgt
    56161 agttggtttc cattagacga gttgttgaat aatgatcact gcctcgaata ttgaaagcga
    56221 tatggccatc cggtccaata ccgcccagaa agaagccaat tccacctaat gcccggattt
    56281 tgctctcgta gttgacgcag aattggtcgg ttcttgcgat cgtttccttt tgtaattctt
    56341 cgatgggatt tttcgcccag cgtgtccgca aactcaagtc ggctttctca tcgccgaaaa
    56401 tttcggcaat actcagatta ttagctgtgc caatttcata gagattaatc aaaagtgcct
    56461 tttctgggtc aagtccgaaa ttcttgatgt agtatttttg tatgtaataa taaaagctgt
    56521 tctgctgatt gggatcaatc ggataaaatt catcaatctg aacaaaatgc aggcgcgcca
    56581 tatccggtcg gcgtgccgga tcaatttcca ccatttcgag atccttaatg acctcagccg
    56641 agtgccaatt ggttagatag tatcccaccc acttgataaa atgctcggga gttttaccgg
    56701 ttggcagagc gattacccca tcgggattat tttgtaccca ttctaaaaac cggagggcgg
    56761 tcaattgacc gagcatcgga aaattcgcta cctgaatgac tgggattttc tctgtcggag
    56821 gatagatgaa ttgcctgcca gagaggctga gatagtactt ttccacattg gaggttggga
    56881 tttgggtgtt gtcaattttt ttaggtggca ttttagtttg cctgcggaaa ttttttctta
    56941 ttcattgata gtcatttcaa atttgaaatt taccttattt tttgctgttt gccaattgtt
    57001 cgtgttgtag ttgtaaaggt tgcgcggtag tctgaaagtt ctcttaagtg cttataagcg
    57061 tttttaagtc tgtttaagtt ctactaacca aatatttatt atgagtttgt cttgaggtac
    57121 gattgtgcga atggatagat gagagggcaa aaatctcaaa aatatttttg attcattgca
    57181 cgtgtttcgc aggctctgaa aattcagttg tgagaaaaat tgtatattgc cccaaaaata
    57241 ctgtaaattt tggcgttgtt atttgttatg gttaaggatc agttgtaacg atgagagaaa
    57301 gacgagccgc gcgcgaagtg gctctgcaag ctttatatgc ggaagagtta accagctacc
    57361 ctatagctca gataattgat gatgtgattg aaaattctga acttcaggat gagttaaaaa
    57421 cttttgccaa ggatttattc atagcggcgg ttacccaccg tgacgaactg gatgaaatca
    57481 ttagagaaaa atcagaaaat tgggattttg aacggattgc catcattgac cgtctggtaa
    57541 ttcgaatggc gattagtgaa tttaaatatt tcaatgatat tccaccgaaa gtctctattt
    57601 cggaagccat tgaaattgct aaaatatatt ctaccgacga tagtagtgcc tttgttaatg
    57661 ggattttgga tgcggtactg cacgatatta gcgatatttt aacttataaa ttgaagcgct
    57721 cggaaaccaa gtcggctact cgagaagaat agtttcggaa aacttgacaa atttccggaa
    57781 acaaattttg agtgttttag tcaaaaacaa taaacataat ttggtaagat aaaatgggaa
    57841 tagctactta cttaacgaaa atttttggaa cctcttctga ccgtgagatc aaaaaaattc
    57901 tcccgctcgt agcagaaatt aatcgtattt atgcaaccct gtctgaacaa ccaatagaaa
    57961 aactgcgcga acgcacagct gagcttaaag ttcaaattca agccgctcgt gaggccgccc
    58021 gaatcgaagc cgaagaatct ggaatctcag aagaggaagc ccaaaaactc atccaaaccg
    58081 ccgagcaaaa cgctctaaat gcaattttgc cggaagcctt tgccatggta aaagaagttt
    58141 gccgacggct ggtggggcaa gaatggttag tcactgacca gaagatagtc tgggatatga
    58201 ttccttatga tgttcaattg attggggcga ttgttttaca tcagggtaaa atagccgaaa
    58261 tgaaaaccgg cgaaggtaaa actctggtgg cgacaatgcc ggtttttttg aatgccctga
    58321 ctggtcgcgg agtccatatc gttacggtca atgattattt agcccagcgt gatagtgagt
    58381 ggatgggacg aatctatacc gagcttggtc tgacggtcgg ctgtattctt aattcgatgg
    58441 attccgaaga gcgtaagaaa atgtacgctt gcgatattac ttacgggact aataatgaat
    58501 ttggttttga ttacttgcgt gataatatgg ctctttcggc tgaagagcaa gttcaacgta
    58561 gctattatta tgctattgtt gacgaagttg acaatatttt aattgatgaa gcgcgcacac
    58621 cactgataat ttcgggaccg gttcaaagtt caaggcatga aaaatttgac gaactcaacc
    58681 cagccgtcgg tacaattgtg cgttatcaac gcgataaaat aacggaaatc ctgagtcgtt
    58741 ttccagattc gccagaatcc attccggaga aagattattc tgaatttgca cgccagttat
    58801 ttatcgcttc tcaagggatg cctaagaatc gccgcttgcg caaattactg gaagaaccgg
    58861 gttatcaaaa aatgttacaa agtggtgaga aggaatattt attacttacg aatgccaaaa
    58921 ccggaacttc aatcagtcag gatgaatttt tcaaggattt atattacttc attgacgaaa
    58981 aaaataataa cataacttta actcctaatg gtgaggaaaa actggctaca ttgttaggaa
    59041 tcaagctcga tgaactcata attcctgacc tggctgagga attcgtcaaa attgacgaga
    59101 atcccgaact agatgaggta gagaaggaaa agcgcaaaat ggagctcgat acccagcatt
    59161 cggaaatctc cgatcggtat cacaatattt cgcaattact aagggcttat accttatttg
    59221 aaaaggatgt cgagtatgtt atccaggacg gtaaagtcct gatcgtggac gaattcaccg
    59281 gccggattct gccggggcgg cgctatagtg acggattgca tcaggctatc gaagccaagg
    59341 aaaaggtcaa ggtcgaagcc gaaacccaaa cttttgcgac gattaccctg caaaattact
    59401 tccgaatgta tgaaaaatta gcgggaatga ccggtacagc cgctaccgaa tcagctgaat
    59461 ttgccgatat ttataaatta gaagttgtgg ttatcccaac ccataagccc tgcatccgca
    59521 tagattacga agatcaagtc tataaaactc gtcgtgaaaa atacaatgcg attattaatg
    59581 aaattgaaac ttcctttcgc catggccagc cggtgttggt cggtactatc agcgttgagg
    59641 tttccgaaac gattagccgt atgttgaaac gacgtggcat tcctcacaac gtattgaatg
    59701 ctaagaatca taaacgtgag gcagaaattg tggcgcgagc cggagaacgg ggtgccgtga
    59761 caattgctac caatatggct ggacgtggca ccgatattaa gttagggcaa ggcgttgttg
    59821 aacttggagg gctctatatc atcggtacgg aacgccatga atcccgtcgt attgatttgc
    59881 aattgcgcgg tcgtagcgga cgtcagggcg acccgggagc atcacgcttt ttcctttcgc
    59941 tcgaagatga tttaatgcga ctttttaata gtgagcgcat tgcggcaatt atggaccggc
    60001 tcggtgtcga agaaggtcag gtcattactc atccgatggt aacccgttcg atcgaacgag
    60061 cacaaaggcg agtcgaggag cgcaatttca ctatccgcaa gaacttgctg gaatatgacg
    60121 atgtaatgaa tcatcagcgt gaaattatct acgcccgtcg caattatgcc ctgaaaggta
    60181 gcgatactaa aggcgaactg tataacatac ttgaagactg ggcagacgac ctggtccaaa
    60241 aatataacgc tcaggttaaa gattctggtg attggcaatg gatggcattg cggatggacc
    60301 ttactaccag tcttggaatt gatttagatc ccagtgaatt atccgaatat acacctgaag
    60361 cgcttaaaca agccattatt gaccgcgcta agcaatatta cgaacggaag gaaaaaattt
    60421 acggcgctga gcagatgcgc tttctggaac gttttataat gttgcgtact attgacgaaa
    60481 aatggcgtga tcacttatat gctatggatc aactcaaaga ggggataaac tggcgcgctt
    60541 atggccaaaa ggatcctctg ctggaatata aaggtgaggc attcgacgct tttgttcaaa
    60601 tggtaaatga cattaatcaa caaaccttgc gactctgttt ccgagcccaa attggcagcc
    60661 cgcaacctga aactcgccag gttcgtcgcc ctgctacgat gcgcttgatg caccaacaag
    60721 ccgacggtat gggtttcatc ccgggaatgc aatcggagac ggaagaacaa ccgcaatacg
    60781 ccaccagtga tggacagcag gctaaaattc gaccggtgcg ggttgaagcc aaagttggcc
    60841 gcaatgatcc ctgcccctgc ggtagtggga aaaaatacaa atactgtcat ggcgccggta
    60901 actgaccggt ggattttttc tgggaactta cattctatcc tatatgatag acgcaagttg
    60961 attaggtcaa attatctaaa gtctctattt ttacacgatt atcattgtta atcactttgg
    61021 aaaatcgcag agtggcggtt aaatttgggc gttttttgag ccgcggtggc ggaattggca
    61081 gacgcgctgg acttaggatc cagtccttaa taggggtgcg agttcaaatc tcgcccgcgg
    61141 cacttctcaa attgaaaaaa taaatagata ggaatttcca tgaaagcgga attaataaat
    61201 agtacaccga tcgagaaaac agttaaagtg gtggttgact gggacgaaat tgccgacgat
    61261 tatatcgcag tatttaacga tttacgccgc gatttgaaaa ttgatggttt tcggaaaggc
    61321 aaagtgccgg aaagtttggc taaacgcctg ctcaagcctc aaattttcgt gaattttact
    61381 aatcagatta tcgaaaaaac tcataagcaa gccttgaaag atgtgggaat ggatgactat
    61441 ctcgatttgc acattgctga actagatttc aatgaaaata caccttttaa ttacactatt
    61501 aaagtcgaaa atgatccgga aattactttg cctgattata aaaagggcat aaaaattcag
    61561 cagaaagaat atattgttga tccggaaact gttgagcgtt acatcgaaga ttttctggaa
    61621 aatcaggcgg aagtgcagga agtttttgac ggtgctcaag atggtgattt tctcgtggca
    61681 gatttgcagg ctctggatga aggcggcatt ccgataattg gcaaacgctt accggatcgg
    61741 ctgattaaag tcggcgaagg gattttcggc gataaggaag ccggtaattt taagggggcc
    61801 aaagccggtg atagtgtcaa cttgaacgtg aaatcgtcgt ctggtaaaac gctaaaatac
    61861 ttggtagatg ttaaacgagt cgaaaaacat accttacctg aacttactga tgaattagtt
    61921 aaaagtcagg tggaggatgt tcaaaccgtg gcggagtttc gtcagcgaat tacggaaaac
    61981 ctgcaatata aatggaatca gcgggctcag gaagaattag accaagccct tgccgattat
    62041 tttattgaaa atacaaaatt tgatattccg ccttaccgaa tcaattccta tttggatagt
    62101 ttaattgaaa aaatgaaaga aaattcccag aataaaccca ttgacgaagc caaaatcagg
    62161 gaagaatatt taccaattgc cgaaaaaaat attaggtggt atcttattca acaggctgtt
    62221 cgaaaacaag aagatattag ggtaagcgaa gacgaaatcc aaacggagat agataaaatc
    62281 ctgcaaattg tgccggaaga gcagaaagag tttgcggaac aattctacca gaaatcccgc
    62341 aatcggctgg agattaagtt aaatctggaa gatcgcaaga ttcgggagca tatcgctcaa
    62401 tttatcaaag gggaaaaagt aaccgttcat accgaatcct tacaataaaa ggaggtaacg
    62461 atgaaaacca aggcccaagc ttatgttcca tttgtcattg agcagaccga gcggggagaa
    62521 cgttcgtatg atatttactc gcgattgctc aaggaacgca ttatatttct tggactgccg
    62581 atagacgatc acgtggctaa tttggtaatt gcccaattgc tttttctggc ggctgaggat
    62641 gccaagaaag acatttattt atatataaat agtccgggtg gctccataac ggctggcatg
    62701 gcgattctgg atacgatgaa ttacattaag ccggatatgg tgacaatttg tctcggttta
    62761 gccgccagta tggcggcggt tctactcgcc agcggtacca gaggcaaacg ccaggctttg
    62821 cccaattcgc gcattatgat tcatcaacca ttgggcggcg tggaaggcca agccagcgat
    62881 atcgagattc acgctcgtga aataattcgt ttaaaggaaa atacgaatcg aatcttggcg
    62941 gaattgaccg gtcaaccact tgaaaaaata aaccgagaca ctgaccgtaa ctattttatg
    63001 tcgagcgaag aagccctgaa atatgggata attgatgaaa ttttaccggc gcggataaaa
    63061 aaataattta tttttataca aaatagaata aaaaaagctt gacattagaa aaataaattc
    63121 tttacattta ctctgcagtg aggtaatgta attgaacaaa caacaatagt attaaaaaag
    63181 gaggatttgt atggcacgtc gtgtagcgat ttgtctttta gcaatgttgc tacctattgt
    63241 tctcttcgcg ggaacctctg gtaaaatcgc aggttcagtt gttgacaaga acacaggtgc
    63301 tcctctagcc ggtgtaaata ttatcgttac cggtggtgga tataatcttg gggcagccac
    63361 cgatctggat ggctattaca gcattctcaa tgttcctgtc ggtacttata ccgtgcgatc
    63421 gagctacatt ggctataaag agacagttta caacaatgtc actgtgtcac tcgatttaac
    63481 cacagagtta aattttgaga tggagcggac tacgttggaa tttgaacaag tggtggtgac
    63541 cgccgaaagg ccaatgatca gaaaagatgc tactaacacc aacattatta agtcagcaga
    63601 agaaattgcc aatatgcctt tacgaggtat gcaagaaatt gctgctagta ctgctggtgt
    63661 tgtcaaagcg gaaaacagca ccgctatgaa tatccggggt ggacgtagtg ctgaaacagc
    63721 tctttatatt gatggtgttt tagtaaatga tccatacaat aatgctgttt acacttattt
    63781 gccgaacgaa gccatcgcgg agatgagtgt tcagaccggc ggttttaatg cagaatatgg
    63841 tgatgctatg tccggtatcg ttgcaattac aacaaacgcc ggcagtgaga attattctgc
    63901 atcattccag gtaattacgg atgaattttt aaaggctgat gagaaaatct tgggtactta
    63961 cggttatggg tttaacgaat atgtagcttc tattggcgga ccaattatac cgaaaagtaa
    64021 tcacacattt tttatcagcg ggacacggcg gtatgcggct gatgcctggc catcctatgg
    64081 gtgggcggaa aatgattatc gtttggatga ttttacgtat gatcaagttt taactaggat
    64141 tgatgatcaa ggtaatactt tcttagatac tattacccat aattatgatc taacagcacg
    64201 cataccaggc aattcaacgt ctgattggtc atggaacgcc aagctcaagt tccagctggg
    64261 taaaaatatg gtattgaaaa ccagctatgt ttctaccgat cgcatcatta gttccgttac
    64321 tccgattgat attttcaata ctgagcatat gggagaagaa agtcgttatt cgcattcgtt
    64381 caatgctacc ctaacccata cgatcagaca gaacacctat tacgacctga aattcaattg
    64441 gtttgatact aagcggaaga tttatgacgc taccttcggt gacgatctgg aaaaatatgg
    64501 cgatccgtgg ttgaatccct ggccggataa cagtgcctat gtcggggttg cttataccgg
    64561 tcgcattaca ccggattatt ttcagccagg ttgtcagtat gatgattatt tcaagaaccg
    64621 gacaacttat tggggaattg attttgacat tgttcaccag atggggaaaa atcatacttt
    64681 taaagccggc ttcgaatata aatatcacac gttgagagaa tatcgggtac tggcaccggt
    64741 ttatctagca aagacaaacc tatctgatct tgaaaaatat cagtctgccg atgtccgttt
    64801 ttatggctat gatattaatg gtaaagagat tgaagaaggt gattttcttg aagatgccgt
    64861 ttatgatgaa actggaagat tagccgatgg ttaccagaaa caggctcctt atcacccgat
    64921 tattctgagc ggctacattc aggataagat tgaattcaga gatttagtgc taaatatcgg
    64981 tctcagatat gatcatatta atccgaatgc ctggcagttc aaacaactgg atgccgaatg
    65041 ggcggctgac ggcagttata taccgggtac tggaatgttt ggtggaaatg aaaaatttga
    65101 taagacagat gtcgaagatt ctgaagtaca tacctatatc agtccccgtc tgggaatcag
    65161 ttttccggta actgatcaaa ctgtttttca cgcgcagtat gggcaatttt accaagctcc
    65221 gagtctgcag gatttatact tgagtccatt ttatttggaa gcctttgtaa gtcggggcgg
    65281 atattttact actttggata acccaaatct taaaccgcca aaaactactt cctatgaaat
    65341 tggttttaaa cagatgctgg gtaacatcgc aagtgtgcag ttgaccactt tctataaaga
    65401 aactgaagac ttaatccagg taatgaacgt tactacggat gtgaccaata ttgccttcag
    65461 tcggaatgga gatttcggaa ctgtcaaggg cttggatatt atttttaatc tcagacgtta
    65521 taagaattta gcggcgacat tcaattatga gctacagtat gcgaccggta ctggttcagc
    65581 gacaaattcc aactttaata tcgcttggca gggtggcgct cgtggaaatt tcccgaaatt
    65641 tgcaatgccg ctggatttcg agcaaagaca tactggtagt ttaaacttag attatcgtct
    65701 cggtagaacc gaaggtccta agtttttaca taatgccggg gcaaacttga tgtttagttt
    65761 caattcgggg catccctata cgctgatgca ggttttcaat actatgcctt ttaccggtcg
    65821 ctatgacaat accaacgttt ccgagacgcc ggtatcagca gttaattctc agacgacacc
    65881 ctgggttttt agaattgatt tgaaactgga taaaaagttt tacattggtg gtgttcaatt
    65941 aacagcttat gcctgggctt tgaatctact gaataccgag aacgtcgcaa atgtctggat
    66001 tactaccggt ttaccggatg atactggata tttagggaca tcggctggcc ggcaatattg
    66061 gaatagttta actgatgagc agaagagtct atataagatg cgcgagatgg attataacca
    66121 ttatggctcg ccgcgtcaga ttcgcctggg actcatgctt gaactataac gagcgaacta
    66181 tgaagataac tcattataat ggaggaaagt atatgaaaaa aatattgtca atatttatga
    66241 taatattaac ggtttcgacg ctcgtaattg gagcagagga tatttataaa acccgggcgt
    66301 tggaaaaagc agccatgacc agtgatatct gggtgaatgc caatcgaatg aatggcgttt
    66361 ttcgaaataa tgggacttgg ttttatgata acgtattagg agattgggga ttggaatggc
    66421 cgaaaggtag tgggctgagt ccaatctttg gtgctggaca atatatcggt gctaaaattg
    66481 atggtgatat tcgtgtcgct ggtgtgcaac actctgcaac agaatttcaa cccggtttga
    66541 ttttagcaac cgatgttgct gataataaag ctgatcctaa gtatagatgg tatgaactaa
    66601 gatctggtgg tgttggtgat tggactacct ggcctaccga tcagggggca ccattagata
    66661 aaaatggaaa tccgatgttg attggcgacc agacaatata tagcgtctgg aatgacctgg
    66721 caccccattc tgaatatggc acaaacaaac tgaatgccga agctcatcaa atggcttggg
    66781 ctttcgatcg tgcgaatgtt atgggagata tgatttttat aaagtggact ttagtcaaca
    66841 agagtggtaa agactgggaa gacacctatt ttgtcatctg gactgatccc gatttaggaa
    66901 atgctggtga tgatcaggtt ggttgtgatt cgactttagg ccttggttac tgttacaatg
    66961 cgaccgataa tgatcaagtt tatggtgccg caccaccagc cgtcggtatt gattttttcc
    67021 aaggtcctat tgtttcttct gacaaccctc ttgatactgt taaactgccg gatggatcgg
    67081 tattccccgg aaagaaaatg cttaagatga catctttcat ctattataat aatgatgatt
    67141 ccaatcaggg taatcccagt tctggaatgg
//
LOCUS       DAOSMP010000008        64885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_478_length_64885_cov_15.827688, whole genome shotgun sequence.
ACCESSION   DAOSMP010000008 DAOSMP010000000
VERSION     DAOSMP010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 64885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64885
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_478_length_64885_cov_15.827688"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PKX09_02350"
     CDS             <1..869
                     /locus_tag="PKX09_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16696.1"
                     /translation="ILIWNCTIKAQFRFEREIVPQSVREGVQFNAISVNEFDDIYLLE
                     SKFSEVYRLDQNGNILNRNGGFGWGLGQFDCPHDLCFSGLDVIVADQNNHRLVRYDRQ
                     LNYIATQDLRSDSRRLIYPMSLTASQINELFILSGETAEILRLYVEKNEQTWFGGIEY
                     GVYALVHPVSLRINRKGILTVLQEDGSLVQFDRFGTPLGLIPFHNSKINKCKAYGLTA
                     VNNDWLILIDQTPFLRMFYTKSKIWDKPTLAGFDRLEPFIATTFSNNRLYLLTSNCTI
                     LVFSMETSQSKP"
     gene            1021..>3664
                     /locus_tag="PKX09_02355"
     CDS             1021..>3664
                     /locus_tag="PKX09_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16697.1"
                     /translation="MKIAASDSVVYLSHRFVIPETIELNVTDSISLDPLTGAITLHST
                     RTDSAFFIAKYQYLPLNLPLNRIVNLPPRIYTGNEIAEPSSTDYPAHPPAQYSTETTD
                     FLKSGTLYRGVTLGSQSGMSLQSGLNLELQGKIAEDISIVGTITDQNIPIEPEGNTQT
                     LDEIDKVFITVVLPHERLTFGDYEMAYSSGSLGNYNRKLQGVYLESERPPMRNVMTGA
                     VTKGQYYHNYFLGEESNQGPYQLIGRNGETDIIVLAGTEKVWLDGRLLTRGESNDYII
                     DYSTAEITFMTRQIITAESRISVDFQYSDLIYQKNIYMAGNTTNLFDEKLRVSAAAIT
                     EKDDRENPIELVISDADRKLLKSLGDNAQDAYISTITSDTNGSYILVDSILIYAGPGQ
                     GTHTATFYNVGKAGSYKKVYRGDVTYFEWVDKNNPLTAASDIAAALYLPVEPLKLPTS
                     RTLYHLASDWQPHQNFAIKAEVARSDFDQNLFSPLDDEDNAGIAVDLTSNLIIPLSES
                     SKIALTGHYKQENWDFEPIDRHQIVEYRRKWDLPSDSTNGEQYYEGQIQYALKNNLVV
                     KTEGGQYSRGDFNSNRLAASAGLSYKMLETVQISEEFIQRNQTGFNDISWTRRGFRLI
                     THLWKVKPFATLNYELREGDTIRTENFRFLEQIYGIGSASPGCLTWQVQTTLRRDDLS
                     DSNTWIKGTNARNIGLNGQLINWHSFSSQWNYIYRIKKFYNQNNSDIVVHLLDFSLKQ
                     EPRKLPYRWETTLKIENEQTVKKEWRYYYVGDGMGDYIYDSTYADYVPDPQGDYILRI
                     LPSSIKVPITSIEDGLRFQFDGANVKMPIFGDWLKRISTLTDVRLRQEIHDPQGIIKY
                     LATDINQVDTSWANYSRIIQQDVNL"
     assembly_gap    3665..3764
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3765..4430
                     /locus_tag="PKX09_02360"
     CDS             <3765..4430
                     /locus_tag="PKX09_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16698.1"
                     /translation="VDTSWANYSRIIQQDVNLRSRERKSDLRLRFYSSDLITQADVRG
                     LEKTFTNEWSLRYRNRYLGKTTIESESALKNYSRQSEINNLRERDIYTVREKLTISTF
                     LDRIHLIGTEITLNSDKAKGSDPIESLLAGVKLSYERKIVGKGRWKSFVELDKVSVTP
                     KGKAIPYEMSNGKKEGLTGGWGTTVEYRVGSNVSIRVNYEGWKEPERTVYHLGSGEVR
                     LSF"
     gene            4430..5956
                     /locus_tag="PKX09_02365"
     CDS             4430..5956
                     /locus_tag="PKX09_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOU16699.1"
                     /translation="MTDQRHRRKNLQIVIFLIIGFVGFLPLLGIAQDTSRAEVIFGEI
                     TVRALEEKLTDFTEITRDYTNYPVTADSYEELTNKILDAVLKDGYLYPVLTLSAVQPE
                     TEGNLTKLNPEFRLDRGERVVIDTVIYEGVKRTAPELLKRESAAILGKSPDLQKISAF
                     LNNFKSYSFLTVQSQPEIVKTREGQFGLVVQLTEHSTNTFSGIVGYIPATATTEGYFT
                     GEMNLNLNNISGRGRSLSVFWAKPNRYSQQIDLRYFEPWIRHTNFNAEGQFSQILRDT
                     LVVLRSFEAGIGRRLFQNGSGALKFNYTSTMPTSGGRQLLGLNDLTTRGLGLSFRYDG
                     RDYPLNPQKGVMVAINAFLSRRIEQNVGRLWQYQTEINGELDVKIRSQLVAACGWNLR
                     GKWLSEGVPVYSDYYWFGGSRSLRGYATELFGGSEVAWLNNEIRWLIGKNGRAHIFFD
                     QGYYRRSVEGLDKKYFPHSYGVGLRLDSRMGVIGLDYGFGEGDTFTTAKIHIFLESKF
                     "
     gene            5956..8100
                     /gene="rnr"
                     /locus_tag="PKX09_02370"
     CDS             5956..8100
                     /gene="rnr"
                     /locus_tag="PKX09_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOU16700.1"
                     /translation="MGKHKNNSPKPKPVKVRTDTQIVERIIQFLQRNKGNIYKQKKIA
                     REVGISSNKYVRFKQILKELAIEGEIENPRSGWYRIPDKARFVEGIISFSGRGFAFVN
                     TDAGEEIFIGADHTANALHHDRVLIEKLRYSNGIRPEGKVVKVLNRGQKSIIGTMKRK
                     NKHWVVIPEHPAAVEEIELVGSEKGLREGQLVEIQDIEWSNARLRPRATLKQILGFPE
                     NPQDDLIIVKKMYRLSDRFPNKAWQEANRLGNQKIKADNRLDLRDKFIFTIDPPDAKD
                     YDDAVSLEKSPDGNWLLGVHIADVSHYVTPGSVLDREARRRGTSIYLGENVIPMLPPP
                     ISDELCSLKPGEDRLAFSVILTLNGAGAVVRYSFAPSIIRSFRRFSYEEVQEILNTKS
                     GEYFDMFTNMRQISRTLFERRRAAGSIDFDIPEPVFKMGPAGIPIEINPSERLDSHRI
                     IEEFMLAANRAVAEYISIKRRNEKLPFLYRIHPQPPQQNITELYQILRNLGLDLHQPP
                     KLKPMDIQKILNGLTEMPYKNFIEQITLRSMAKAIYSHQPLSHFGLAFPHYTHFTAPI
                     RRYPDLVVHRLLKNYLGDYDSNDLAFYRRSLPHIASSASQREIEAMEAERTYQKIKQV
                     RFMADKIGRWYKGVISGVREFGFFVEISQFLVEGLVHIRTLSDDYYIYDPENHLLRGK
                     YSQRIFRLGDEVMVMVTDVSVRERRIDLEWCE"
     gene            8171..9190
                     /locus_tag="PKX09_02375"
     CDS             8171..9190
                     /locus_tag="PKX09_02375"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HOU16701.1"
                     /translation="MNKKIGLLVLIISGMLMNCNQLRESIGGVDDIYIFATDQTREVL
                     GKAIDTTFSYGIRTPEFQPYFSTKWLPVEEFPSYIKFKNIILVADLTQDDLAAEIARG
                     ILPKQSLIAAEKDSIHMFAIPDNWARDQMFVLIAGRNLKMVERSILEQKGWLYGKFEE
                     KFEEVQMDYIYGRYEQKKLTNYLWQKYRWTMRIPHDYIIIRELPENNFVWLGRGLPYR
                     WISVTWGDGIQTEWLTNVGLYEKRNEIGKLYGGIMTDKRFLGFQFFKFGEYDALKMYG
                     LWYHTEETQGGPFETYAFYDWRTDRTFIIDMLMYGPGEKLSVLFRGLELTAKTFTTDY
                     KGSWK"
     gene            9266..9727
                     /gene="purE"
                     /locus_tag="PKX09_02380"
     CDS             9266..9727
                     /gene="purE"
                     /locus_tag="PKX09_02380"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOU16702.1"
                     /translation="MEKIALIVGSNSDLETVEQARKYLEYFELDCEIKVLSAHRQHDE
                     LVNFVKRFEKENGRLIIACAGMAAHLPGIIAALTTLPVIGVPLNASSLNGVDALYSIV
                     QMPGGIPVGTMAIGKPGIINAIIYAARILALSDAKLKAKLVEFAAAGFKLN"
     gene            9730..10641
                     /gene="rfbD"
                     /locus_tag="PKX09_02385"
     CDS             9730..10641
                     /gene="rfbD"
                     /locus_tag="PKX09_02385"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOU16703.1"
                     /translation="MKILITGANGMLGEKCTRLLAVKHRVLATDLHDKLIYKLETDYR
                     KMDITKLEEIENIISEFQPDWVVNCAAYTNVDGAETDRETAWQVNAVGPMNLVAALRK
                     SNTPIIQISTDYIFDGVNGPYRENDPVKPINYYGETKLAGERAVLESELPATVFRTNV
                     LFGNSTNQEASFVYWVVNKLSQREPINVVNDQFGNPTWADGLAQVIEMAIERGIHGLY
                     HYGGADYINRLEFALQIAAVFELDPTLIRAVSTRSLNQKAPRPFRAGLICEKIQSEFG
                     IHLYSVKEALTNMKEKQFEVTSNHTNI"
     gene            10610..11326
                     /locus_tag="PKX09_02390"
     CDS             10610..11326
                     /locus_tag="PKX09_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOU16704.1"
                     /translation="MKSLVIIPTYNEKDNIRTVIERLQALPIELDILIIDDNSPDGTA
                     DIVRELQADDPHIFMINRPAKLGLGTAYITGFRWALERDYEYILEMDADLSHNPDDVP
                     RLIQECENGYDLVIGSRYCNGVNVINWPIKRLLLSYGANKYTRMITRMPIMDATAGFK
                     CYRREALAAINLDRVKSSGYSFQIEMHFRVWDAGFKIKEIPIIFIERSEGLSKMSKNI
                     VWEAVFMVWKLKLRQIFRSL"
     gene            11329..13398
                     /locus_tag="PKX09_02395"
     CDS             11329..13398
                     /locus_tag="PKX09_02395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU16705.1"
                     /translation="MQISVIIVSYNVKEFLQQCLLSLKNALEGLSSEIIVVDNDSVDG
                     TVNVIKNKFPEIILIENHFNKGFAAACNQGLAIARGEYILLLNPDTMLQEDTIKVMIE
                     FFRAHPEAGAAGCKILNADGSLQLACRRSFPTLTVALPKLLGLSRLFPKVKAFSRYNL
                     TYLDPDQITEVDAVSGSFLMMRRAVYEQIGGLDESYFLYGEDLDYCYRIKKSGWKIYY
                     VPDTKIIHYKGESAKHAAFDNFIAFYRAMAIFASKHLGKSSSIITDIVFKVGIFFRGI
                     ISLVAKFFRRRMVMIVDGIALVAAILVAHQLQPKPLPDYPSLLSMLVFYLLVWLGIGY
                     SIGLYDRRELSYSRAVVASLLSFVASLIFNLIFKDVIYSPNMLLLSFIIVTLFLPGWR
                     VLLLFLQRRRVIQPASSLSKALLSRRTIIVGAGKEGERIARKLKTHIEHGFEILGFVD
                     KDYMNEEIAGFPFLGLVSDLPEIIRINHATEIIFTTDRFNNDDILGIVDKIKKYRTNI
                     KIVPRNLDYILGKSSVENIEDIPLVNIDYNLFHVGNRVTKRIFDLLISSISIILLSPF
                     VIPIAFLLGYRFQKANFIGAEQHQISALTLVKRNGNHVHPFLNRFPLLFSVVKGDISL
                     VGSELVPAENGERNLRFKPGLTSLFRVSKNHRTDENDKQNYEHFYMQNHSLFLDVEII
                     LKTVLKI"
     gene            13403..14386
                     /locus_tag="PKX09_02400"
     CDS             13403..14386
                     /locus_tag="PKX09_02400"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOU16706.1"
                     /translation="MMAEIVLDFEKPIIELEKKIEELRAQSAASGVDLNPEIEKAKLK
                     LNRLIQNTYSNLTRWQRVQLARHPERPYTLDYVKRICSEFIEIHGDRYYADDKAVVAG
                     LAQIEDYKVILIGQQKGRTTKENLYRNFGMPHPEGYRKARRAMKLAEKFKRPVISFID
                     TQGAFPGVGAEERGQAEAIARNLFEMARLRTPIIVVVIGEGASGGALGIGVGDRILLL
                     ENTWYSVITPEGCASILYRDSSKAPLAAEAMKVTARDLAEFKIADEIIPEPLGGAHRN
                     PDQMAQTLKDVLLRYLAELTQIPIDQLVDQRIEKYARMGSWKVVDVKSKSK"
     gene            14408..14818
                     /locus_tag="PKX09_02405"
     CDS             14408..14818
                     /locus_tag="PKX09_02405"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOU16707.1"
                     /translation="MLEHHYQLRVRYSSVDQLGVVYYSRYLEYFEAARDEIMRAIGLP
                     YSQFEQAGYAMPVVEAHCVYHRSAHFDDLLTIISRISAIPTARLRIDYEILRENESQP
                     LVSGYTIHAFVNQSGKAVRPPQFFLKLLAAQMSK"
     gene            14863..15495
                     /gene="tmk"
                     /locus_tag="PKX09_02410"
     CDS             14863..15495
                     /gene="tmk"
                     /locus_tag="PKX09_02410"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOU16708.1"
                     /translation="MTKGLLITFEGIDGSGKTTQIKYFIKKCERENIPVALFREPGGT
                     PIGEKIREILLSKSNSEMFAITELLLYSASRHQLCRELIRPALESGKIVVCDRFYDST
                     TAYQGYGRGIDLNFINMLNRIAADNLVPDLTFIFDIPVAERLQRLGTRDLDRLEREEY
                     NFQEKIRQGFLKIAAAEPNRVCLIDGTKTEELVADEVWHKFNKIIRKMQL"
     gene            15492..17138
                     /locus_tag="PKX09_02415"
     CDS             15492..17138
                     /locus_tag="PKX09_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOU16709.1"
                     /translation="MKLSFKKGLKIWIAVLVSVSVLLAFTAKDIYKTISDSYKLYNAV
                     FQQILLNYADPLDAAALTESNIRNMTRELDPYTVYMTEEEKEPLEILSKGEYGGVGLR
                     ISLRNDTLTVISPIEGSPAKKANILPGDQILKIDTIWTTGLDLDKCTRLIRGNVGTKV
                     ELVIRRPGADEGKVYILERAKIQVYDVYYADYLEEGIGYIRLAEFSKGSTAEVRQAIE
                     NFKDHDQLKALILDLRGNPGGLLTEALAVAELFTQPGDTLLFTRGQNESSNRVDVARR
                     RPLLDESVKIAVLIDGGSASASEIVAGIIQDLDRGIVLGTPSFGKGLVQTVFRVDKEH
                     SLKITTAKYYLPSGRLIQKPDYIKNPKLIAASPSQDSIFYSRHKRQLKSKGGITPDII
                     IKSDEQSDFITELWRQNQFYSFAVKYKTEHNELPEEVNELIIKEFKNYIDHSGFQFIE
                     KTEKNIREMDKIISQDERLKNVKIDFQPVYEIFDSLKEAEFENNHLMIQRGIENQFAM
                     LRDGIAGQLKADLKYDKVVSEAILAVRDDGKYKTTLGLAR"
     gene            17147..18286
                     /locus_tag="PKX09_02420"
     CDS             17147..18286
                     /locus_tag="PKX09_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOU16710.1"
                     /translation="MRTTIQLFALMLFIFSIGKICQADTTEVKQQIETIIAEIRKKYC
                     PDQRVALWKIEIDYQDNDYNLVGETDHPEAVRALTNQIKQTLPAIKIQNLIKILPDET
                     VNGKNFGIVINSVEPLRSGPSVFNDMVTQTLRGLMVEILKSTEGCFLVKTDDGYLGWM
                     EADRLVVGGDSLKTAWENTPKVVYDDFEGIIYSKADQKSMPVADVVLGNRFKMIDKQG
                     QWTRVEIPDGRRGFIPSQQLVSESEYLARTPTAEGIITTAKNLLGRPYLWGANSPKAF
                     DCSGFTQTVYRRNGYLLARDANMQVNQGFAVDTTNLLENLMPGDLLFFGPNPEQINHV
                     TIYIGDYEYIHCSAWVRINSLKPGAANYNDYLLKKLRYVRRIIGK"
     gene            18705..19511
                     /gene="nagB"
                     /locus_tag="PKX09_02425"
     CDS             18705..19511
                     /gene="nagB"
                     /locus_tag="PKX09_02425"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOU16711.1"
                     /translation="MRVIIHNNYEKLSDWVARYVAAKIIHANQTSAKPFVLGLPTGSS
                     PIGTYQALVRLYKEGKVSFKNVITFNMDEYVGLSEDDPESYHYFMRHYLFNHIDIPPE
                     NINILDGNAKDLEKECEEYEAKIKRVGGIDFFLGGIGVNGHIAFNEPGSSISSRTRIK
                     TLSYDTIVANSRFFGGDLSRVPKTALTVGVGTVLDAREVMVIASGYTKARALQKVIEE
                     GVNHMWTVSMLQLHPHGTIVCDDEATLELKVGTVEYFKDIEDEAINNLPV"
     gene            19527..21497
                     /locus_tag="PKX09_02430"
     CDS             19527..21497
                     /locus_tag="PKX09_02430"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOU16712.1"
                     /translation="MENRQLTQTEINLLEKQGCRAENWEKITVKPDFNPANVRNVTFA
                     GAVEIGNCCGVQEVKPGCLKNCGIYDSYIRNCTIADNVYIANVKNLINYTIEKDVIIE
                     NVGDLIVTGETTFGNGTELEILNEGGGRILPIYDRLNAQIAYMIVVYKHDPALQKALK
                     KLVSDYAAERKSSRGVISQGVRIYNSNIIRNVQIGEKATVSGALLLEDGTIAGSEVDP
                     VYIGEGVIAKHFIIQTGARVDSSAVLDKCFVGQGTRLGKQFSAENSAFFANCECFHGE
                     AVSVFAGPYTVTHHKSNFLIAGMFSFFNAGSGTNQSNHMYKLGPLHQGVLDRGSKTGS
                     YSYLVWPARIGIFSVVIGKHFAHLDTSELPFSYLIESEDKSILKPAMNLFTSGTRRDI
                     IKWPARDRRKDTDRLDLINFDFFSPYIIEKALRAIEILHRLDESSQPDQEFVKYQGAY
                     IRKSVLKGAIQNYELIPKIFYGNLIMQKIGSIDNLTWDKVKNIFTGEADKSAENWEDL
                     SGMFATSAEISKINFALRENRLNSIVDLECALRDVHQNYEENTWQFCLNLLFKRFATN
                     ALTSEQVCQLLDEWQASVVRFNNLVLKDAEKEFAQCSRVGYGIDGDAIIRDQDFEAVR
                     GTFAENKFVVELKKESEEAVKKVERICAALRK"
     gene            complement(21563..23194)
                     /gene="pruA"
                     /locus_tag="PKX09_02435"
     CDS             complement(21563..23194)
                     /gene="pruA"
                     /locus_tag="PKX09_02435"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOU16713.1"
                     /translation="MSNAKFTLPQPVNELQFSYLPGSSERQALKIALNKIAGEQIEIP
                     LIIGGKEIHTGQTAKCIMPHNHQHILAVYHQATAKEVSLAVEASQKAWEFWSVMPWHE
                     RAAIFKKAAALLSGPWRMTLNAATMLNQSKNVYQAEIDSACELIDFLNFNVKFMEQIY
                     EQQPLSSPGQWNRLEQRPLEGFVFAVTPFNFTSIAGNLPTAPAIMGNTVLWKPASSAV
                     LSAYYFMKLLQEAGLPEGVINFIPGAGRNVGPNVLSDPLLAGVHFTGSTAVFQSMWKT
                     IGENIANYRGYPRIVGETGGKDFIFAHVSADLDELNTALIRGAFEYQGQKCSAVSRAY
                     IPQSLWPSLKEKMLASLSTIKIGDVRDFRNFINAVIDKTAFDNILSYINYAKNSRDAE
                     IIAGGKGNATNGYFVEPTIILTTDPHFKTLEEEIFGPVLTVFVYDDNKYEETLNLCNM
                     TSPYALTGAIFARDRQAILLADKFLLHAAGNFYINDKPTGAVVGQQPFGGGRASGTND
                     KAGSMINLLRWVSPRATKELFVPPKDYHYPYMIEE"
     gene            complement(23556..24587)
                     /locus_tag="PKX09_02440"
     CDS             complement(23556..24587)
                     /locus_tag="PKX09_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006893319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOU16714.1"
                     /translation="MKAYFISEIKDLKTTETPLTIADVEQPRPTSNDILIKVSVCGIC
                     HTELDEIEGRTPPSSFPMIPGHQVIGQIIENGPDARRFAIGTRVGVAWIFSACGKCSY
                     CLSDQENLCPQFRATGRDTNGGYAEYMTVDENFAYPIPEIFTDAEAAPLLCAGAIGYR
                     SLRLTNLYNGQKLGLTGFGASAHLVLKMVRHRFPQTSVYVFARSENEQAFARELGAVW
                     AGDTNEPAPELLDAIIDTTPVWTPIVAALKNLMPGGRLVINAIRKENSDKSILTTIDY
                     PEHLWLEKEIKSVANVTRRDVAEFLELAAEIPIKPEVEIYPFEKANQALLDLKQRHVR
                     GAKVLQISY"
     gene            24894..25970
                     /locus_tag="PKX09_02445"
     CDS             24894..25970
                     /locus_tag="PKX09_02445"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HOU16715.1"
                     /translation="MQKEHKYNIERKNILRSFTLIFLLSFIFQIPKCDENIIDEKEAN
                     NTWTKVESGVSACLYDIDFVNEKNGWVVGDSGVILNTKNGGETWEKQLCLTEETLFAV
                     DFIDTNCGWICSRNSIIRTTNGGKSWEIKYSEDLGEGRFRDIQFLNMNTGYVVGGRGA
                     FGGQGVLTKTEDGGETWQDVTPQDINTLTHISIVDKQNIWICGFGGTILNTTDLGLTW
                     IHQDLNVSSAQYLTAIQFVDQYNGWVGGDDNSWLGFFRTTDGGSTWTQRSPDSWPVFL
                     GVQSFFFVDSLYGWLATIPGAGPYAIIQTHDGGQTWEFLPEDMNIYDIRSFYFINKEL
                     GFAVGLEEVNTNAEGVILIYRSIE"
     gene            26117..26296
                     /locus_tag="PKX09_02450"
     CDS             26117..26296
                     /locus_tag="PKX09_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16716.1"
                     /translation="MAEQIPSANLFSMPTEDLIQEYLNSRYPRYILIYNDIQNAFEHA
                     YNDFNGFRELPITLI"
     gene            complement(26339..27367)
                     /locus_tag="PKX09_02455"
     CDS             complement(26339..27367)
                     /locus_tag="PKX09_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006893319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOU16717.1"
                     /translation="MKAYFISEIKDLKTTETPLTIADVEQPRPTSNDILIKVSVCGIC
                     HTELDEIEGRTPPSSFPMIPGHQVIGQIIENGPDARRFAIGTRVGVAWIFSACGKCSY
                     CLSDQENLCPQFRATGRDTNGGYAEYMTVDENFAYPIPEIFTDAEAAPLLCAGAIGYR
                     SLRLTNLYNGQKLGLTGFGASAHLVLKMVRHRFPQTSVYVFARSENEQAFARELGAVW
                     AGDTNEPAPELLDAIIDTTPVWTPIVAALKNLMPGGRLVINAIRKENSDKSILTTIDY
                     PEHLWLEKEIKSVANVTRRDVAEFLELAAEIPIKPEVEIYPFEKANQALLDLKQCHVR
                     GAKVLQIS"
     gene            27934..29040
                     /locus_tag="PKX09_02460"
     CDS             27934..29040
                     /locus_tag="PKX09_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16718.1"
                     /translation="MANHSKFLQVLSYTRCQLIILSAFIIFIISCDDNTINSNPSNSP
                     DLCYQKMDDNSHWQIFTNNISGTNPENISNNPNNDCYDPAWSPDGQYIAFRWDKSVGG
                     CDIYLYDIFSDSLINLTTDLSATESASLPEWTPDGEKIVYTQRNNGINYQYIMNKDGS
                     DKRKLNDWINFFYNDSYHIICQRDNSIFKSNIINSVNEFIIDIKAIGEEYTKLYDFNP
                     NTEDLLFLIADTPRKTNIIAEYNVQTQKLDTLSVADSGWVYLRPQYSNDYAKIAFIER
                     NYDKDISNMVIIENGVKKDLVTLTDEDEWIYNSPIVFSPYDNYLAYSKNINQEGYLVW
                     WKSYLYIVNIETKKIKFIDGESAVDPVWNPLLTY"
     gene            29586..30884
                     /locus_tag="PKX09_02465"
     CDS             29586..30884
                     /locus_tag="PKX09_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16719.1"
                     /translation="MKKIIVLLFVMISIIQPQTIKKVVQFSDSESIDILTESENYVGA
                     AISVNSFQALPRAILFNKNGDIVFEIEDPKRSGFIVVEPVESLGYFITVRRGGGKVLD
                     QIRAFDLNTGHKVWETNANACAFELSPNKKFMITVGDPGTERTGIFEIINLKDGSKVD
                     FGYNYDQYCAAWLDDERIIFVFQEWEKGVKQNVQKDSVQSHRWKEIEFTKKSFQLKYK
                     YDQGLISETEYEKGKKELDELQMELEKTKPKMKRTPSGGFIEARGPVTMTPKTIKVIL
                     YNLTTNSIDREEYLYKGDGSKISIGVSPNGSKTINVDKEKNIYIYGYDIIENELHSYL
                     VKFTENLNFIWTTTAEIANIITFFVDNNVYFRTKIGNDVNLIDTNTGHLLPVDEAKKK
                     NSKINDIDFSYRHRIYKVKILRNTNVDYQNKTITFSKVED"
     gene            31347..32174
                     /locus_tag="PKX09_02470"
     CDS             31347..32174
                     /locus_tag="PKX09_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16720.1"
                     /translation="MNNCGKCTFILLLLILCIYGVSNSQIEQPSIPARDKPFPLINKF
                     PPAIQRLDSIKVKYYKEKKELELQGKKYEYSDKFLKDYARADISSRLFYSKIDGSGLE
                     REYKLLTGPIGTETMKLLKQLPYPWGILIEKHGIVLAEVLNDTVLPKMYYPVPRPESA
                     SKVNNYVLKAKIIDDIFGTIEEDTVLIRHNHLITENILFNQYKDVKVIFSLKPGGTVQ
                     REGYVERIYLMGTPTEFMYVADGIIYDVNDAFNKNGITYQEFKNELLDFIKSEGIRP"
     gene            32546..34411
                     /gene="htpG"
                     /locus_tag="PKX09_02475"
     CDS             32546..34411
                     /gene="htpG"
                     /locus_tag="PKX09_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOU16721.1"
                     /translation="MPKKKTDENQTYEFKAELKQLLNLIIHSLYTQPEIFLRELISNA
                     SDALNKVRFMQLTNQNILDPERELKIIIEVDPKKRTFSIEDTGIGMTREELIENIGTV
                     AKSGTLQYLEQIQKEGKQIDADIIGQFGVGFYSVFMVTDEVTIETRHASPDSKAYLWK
                     SDGKGTFSISEIIRPERGTRISFTLKEEYKDFAEEYYVKSIIQKYSNFINFPIYVGKE
                     RVNSIEALWRKPKDEIKEDELIEFYKFLTNDINPPLGHLHLSLEGSVNFKALLFVPSS
                     TPYDIFYEIREKSLHLYANRVFVQDNATDLLPEYLRFIKGVVDTEDLPLNISRQVTQD
                     SPVMAKIRNVLTTRILAMLEDWAKNDVSKYEKFYKNFGHIFKLGVTTDFANKDKIIKL
                     LRFETTAKNKGELTSLDEYIARMPKDQKEIYYLTGENREIAERNPKLEYFRKHNLEVL
                     LLVDPIDIFIIPTIYEYEKKKFQSIEKADLKLEKKAQPAEGEALEENLLKSLIAVFKE
                     TLGDKVEDVIVSSRLVDSPATLVVGKEGLEPQIEKFMKMMNQEIEPQKKILEINPSHP
                     LIKNLSRLNLGNSHDPLLRQSILQIYESALLLDGNLENPTEFVSRMVDILTTATK"
     gene            34667..35638
                     /gene="obgE"
                     /locus_tag="PKX09_02480"
     CDS             34667..35638
                     /gene="obgE"
                     /locus_tag="PKX09_02480"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOU16722.1"
                     /translation="MFVDYTKVSVQSGSGGKGCVAFRREKFVPKGGPSGGDGGRGGDI
                     IVKVDHNLNTLQDIKYHKSYKAQNGEPGQGGLKSGADGAPVIIRVPKGTLIRDELTNK
                     ILADLVEDNQEVVIARGGKGGRGNAHFATPTKQTPRYAEPGLSGEKKKLIFELKLFSD
                     VGLIGLPNAGKSTLISKITAARPKIADYPFTTLTPNLGIVKFGEYKSFLIADIPGLIE
                     GAHQGKGLGFRFLRHLERTKVLAFLIEATDPEPELTYQMLNTELVKYLSTFENKPRFI
                     VITKTDLVHKLPTLKFDNLPIVPISAVTGEGLELFIQQSLKLLAQNG"
     gene            35631..36728
                     /gene="dprA"
                     /locus_tag="PKX09_02485"
     CDS             35631..36728
                     /gene="dprA"
                     /locus_tag="PKX09_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOU16723.1"
                     /translation="MDKLQAILSLMSINGLGSHRILSLYRQYPDIQEVFSASPKRLIE
                     VPGIDFKIAERIKNNVDHQFVDQQLNWLKNTPYKITTIIDEDYPARLRNIYDPPVVLF
                     RYGEFLPQDEDAIAIVGTRNPDHYGKEVAALLVSQLVEQNITIVSGFARGIDTIAHKT
                     ALEEGGRTIAVLGNGIERVYPPENRSLRDKLVTQGVYCSEFPFGTKPDAVNFPRRNRI
                     ISGLSLGTVVISAGERSGALLTAYYALDQNREVFAVPGRITDEKSFGTNHLIQKGAKL
                     VTSVGDILDEIDAIRRFPRQPRQLEISFEFENEDEKAIYEALSTDPIHIDDLAQKLGK
                     NTYSILATLLSLELKGAVRQLAGKMFIKAGL"
     gene            36729..38429
                     /locus_tag="PKX09_02490"
     CDS             36729..38429
                     /locus_tag="PKX09_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16724.1"
                     /translation="MQIFKEIRGNFGLIFSICISILFLPQKSPSQDIFDRPVRLYQLW
                     DWISYKYSNYPTSLTEGVELVYFGTSGGIYAFHKYGRYWTEPFTTSNGLSDDFITAVL
                     YDETTRYLWAAHNKGVSYLTPSSFRWTNISKADLNLPETNRILRLGTDGNAIWVQAEG
                     DYLLTINKILGFYQAEEESASEAVKWQPTSLDGVPDMRGYTIDQDYQIDGRGLILDQN
                     LREYRVTVFAKINNDIYGGIAGLGLFEGNANIKTLHIHPFGPMQNYINAMAVDDNRIW
                     VGGTRSDLNPIFTKSGISCYNQNDGFWEYFESLFINEFATPVVNDLAYKNNRLWIGTE
                     QGLSIYDQNKNRWKRLSMSSGLADEIITSIALEDTLAWIGTPRGLNLISIPSYKARRQ
                     RLSPRQNILKIFKIAIDSKNIWIGTDNGIYSIDKFNHNVEHYNMFGERIGLDDAVAGN
                     ITAIGCGDSVTVFGRYHDLLQFSYNERKWTNIPLTDQLNEASFFDIAIDGNYMWLGTD
                     RGAFLMRLSDYYCENYTTLDGLAGNNVFKIFIDGDWVWFATDCGLTKYSWRKYVTLTE
                     "
     gene            38410..39681
                     /locus_tag="PKX09_02495"
     CDS             38410..39681
                     /locus_tag="PKX09_02495"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOU16725.1"
                     /translation="MSHSLSKISLLVCGLLTVALAQEPADFRGNVEPEIPNFIYDVSI
                     TPIVTRDSIKIDIIIKVPYSAIQFVKKDTIFIGKYEISVMLLDEKEVNAISKIWTKTL
                     QTKKFEETHSNELYDINKLTYKVKPSDYSLTIGVMDLDTRKTTHRKRELKLKNYYKKS
                     ITLSSINIIEDIVADSSGDSTDVSALTGTLSNKSEKFDISFFVLSNGGKGTIKYSIYN
                     MNKKVVLENKIDHNFKKGVEYQRISIPRQKLSYSKYRLVLTVKIGSEEVTAEKIIQIR
                     WTGMSDMINNLDEAIEQLRYIARGDVISKMKKASGEEKKNLFLEFWRERDPTPGTEEN
                     ELMNEYYRRVAFANQNFSGYLEGWKTDMGMVYILFGPPNDIERHPFEIQTKPYEVWYY
                     YELNRTFIFVDETGFGEYRLITPLDYYGSFY"
     gene            39681..40760
                     /locus_tag="PKX09_02500"
     CDS             39681..40760
                     /locus_tag="PKX09_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOU16726.1"
                     /translation="MLKSRKIAVIAGDGIGPEIIRQVVKIFEVLKNYSQINLAITHAP
                     YSAEYYLNTKVAIPDEYIAELEKNYDAILIGPFGDPRLPDGRYAREIIWGIRNKLDLF
                     FGYQRVKVYDSWMSPLKNTQEGLADFYIIRENLESINPKPGGSYYRDTNKEIAIQSSI
                     YTRQNIDRFIQQAFEFAGKRNYKKIVLAHKNTLMTNLHELLLKIFQEKITAFPEINAD
                     VMNIDALLFELLSDPAKFEIIVAPGDLADSIYTAGLFLQGSYGMAHICEINPVKIGAF
                     RIAQGSAVKIAGHNRANPLGAFIATIEMLKFIELDEAAEAVERALIQILQKHLVTIDM
                     GGLIGTEELGNYFCEFLKEQLSISR"
     gene            complement(40763..41173)
                     /locus_tag="PKX09_02505"
     CDS             complement(40763..41173)
                     /locus_tag="PKX09_02505"
                     /inference="COORDINATES: protein motif:HMM:NF022443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HOU16727.1"
                     /translation="MTLRQLFKRTYAIKILIILIIVPIGFYTKFYSGPLQKWVSYSSG
                     DFWYEIFWCLVISLFFPNLKSSRIAIIVFFATGILEFIQLWHLPILENIRDTFVGRTL
                     IGNYFIWSDFIYYAAGCLAGYFILEAISRVKTAK"
     gene            41379..42809
                     /gene="gatB"
                     /locus_tag="PKX09_02510"
     CDS             41379..42809
                     /gene="gatB"
                     /locus_tag="PKX09_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOU16728.1"
                     /translation="MSEKFEPVIGLEVHAQLLTDTKIFCSCKNAYGAPPNSLTCPVCL
                     GMPGTLPVLNRKAVEYAIKMGLATDCSITAFSRFARKNYFYPDLPKGYQISQYDEPLC
                     QNGKIKIQVNGIEKNIGITRIHLEEDAGKSIHGFDNTLVDFNRCGVALIEIVSEPDLR
                     SPEEAYAYLIKLKQILEYLEICDCNMEEGSLRCDANISLRPIGEEKFGTKTEMKNMNS
                     FRGVERALRFEIERQKAILEKGKNIIQQTMLWDEAKGEARPMRSKEESHDYRYFPEPD
                     LVPIEVEENWKTKILNDLPELPDQKYFRFLNKYNLRHYDAAILTSDKYLAEYFERVND
                     LINDPQLVSKWIQGEVLRTLSQKNLTSKQIPLAAENLGELLNLIKAGTISANIAKEVF
                     DKMVENNESAKIIVEREKLVQVSDDSELEQIIQTVISENPNEVERYRRGAKNLFAFFM
                     GQVMKKTKGKANPGVANNLLRKALDN"
     gene            complement(42928..43015)
                     /locus_tag="PKX09_02515"
     tRNA            complement(42928..43015)
                     /locus_tag="PKX09_02515"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42979..42981),aa:Ser,seq:gga)
     gene            43147..43479
                     /gene="cutA"
                     /locus_tag="PKX09_02520"
     CDS             43147..43479
                     /gene="cutA"
                     /locus_tag="PKX09_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOU16729.1"
                     /translation="MKKDVYLQIITTTDTKEEAEKIAHILVEKGLAACVQVIGPVTSV
                     YQWNGKIEISAEWICQIKTIKDLYNSVEKEILTHHKYELPEIIAIPILTGSKKYLDWM
                     RMQVTTND"
     gene            43787..45199
                     /gene="glnA"
                     /locus_tag="PKX09_02525"
     CDS             43787..45199
                     /gene="glnA"
                     /locus_tag="PKX09_02525"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOU16730.1"
                     /translation="MTSKEFFKFAKQNDAKMVDLKFTDLLGTWQHCTFPINSWGENTF
                     VEGVGFDGSSIRGWQGIHLSDMLAIPDPDTAVMDRFFKEPTVSVIADIIDPITRENYS
                     RDPRFVARKGEAYLKKTGIADTCYIGPEPEFFIFDEVMYEQNQHTSMYLINSIEGAWN
                     TGRFEEPNLGYKPSFKGGYFPVSPTDTYHDLRTEMALELQKVGIAVEVHHHEVATAGQ
                     SEIDMQYMPLTKMADQFMWFKYIVKNIAKRHNKTVTFMPKPIFQDNGSGMHTHFSLWK
                     NGKPLFADNGYAGLSQIGLYSIGGILKHAPAILAFAAPTTNSYRRLVPGFEAPVNLVM
                     SARNRSAAVRIPMYSNNPKAKRLEFRCPDPTANGYLTWTAMLMAAIDGIKNKIDPGEP
                     LDRDIYEMTPQELEKYPKTPKNLAEAIDALEKDYEFLIADDVFTTDLIQTWINWKREK
                     ELYELSLRPHPYEFYLYYDC"
     gene            45394..45981
                     /locus_tag="PKX09_02530"
     CDS             45394..45981
                     /locus_tag="PKX09_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU16731.1"
                     /translation="MKCENITRDKILMTAARLFADKGFEATSMREIAESCELTKPALY
                     YYFQDKNTLFAEIINAIANYSVQFLTEIYQSDKDPINKLYDIVIMQVTRLKQNPEITK
                     FLINAPIRNIPNETKVEFYKQMQKNKEMLIHIITEGKEKGYLKSDLDVNAFLYCYVGG
                     INQFIIDHIKNGTENLNEQNANVIVRTLLDGARNH"
     gene            46013..47359
                     /locus_tag="PKX09_02535"
     CDS             46013..47359
                     /locus_tag="PKX09_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU16732.1"
                     /translation="MEFKLKVGVVFLITIASLFAEELNLDIETAREIALQRNPSLKLA
                     REAVLKSRASVNEVRGSMLPSISAFSQFKHAWEVGGMPTPQEFKDLITLLGGTPQETM
                     SFERENTFVAGLNLSQPVFTGGLILNSYQMSKIGVRITESQLKATEQKILSDVTNAYY
                     GVLFANSASEVAEEALHSAEQNLAQVQKFFDVGKASRFDVLRAEVQVANFKPMVVSAN
                     NAKKLAESSLRLILGIGEDININYLEKLSYKESDLINMTLDELITIALAERSEVIMLE
                     DRKKLAEKQVNLAKAKYSPSLVFGTAYQYQAFRDDFDFTTDDLVKSFNSSISLSIPIF
                     SGFATKAKVQQAKIAVKEVDYQAESAIDGIKLEVKSAYFKMKEANENVQTQEKTIEQA
                     KEALRLAELMYSEGASTQLDVLNANVALNQAKMNYQKSLYEYNVALANLKKAINQL"
     gene            47374..48516
                     /locus_tag="PKX09_02540"
     CDS             47374..48516
                     /locus_tag="PKX09_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU16733.1"
                     /translation="MKLSKNILNLFLVCGIIYYINSCSKTQTDEQMTENIVPVNVVTA
                     ERGTIEEKINFLGNIKGFREIKIYSLIPNKILKINVDVGDWVNKGAVLAVVESDKIEQ
                     AVKQANAGLEAARAQLRNIETEWNRIKKLYDDNAVSKSQYDAITTQRDAARANIKQLE
                     AAVSTAKKQLEDTQIKAPIAGIVSSRLLDVGDQAAPQIPVFTVVEMDDVKITIDVIES
                     QFNKVKIGQRAYINVDTYPDTVFVGEISKISPTLNLMTKTSPAEIIISNPEHKLRPGM
                     FARVDVITDVHKNALIIPRYSIIERTSLEYIGGDISSTKTIINKYVFVVESGIAREVK
                     IETGIENGRIVEVLQGLNDGDLIVSMGQHNLSDSTKVEIIEKGKAI"
     gene            48513..51659
                     /locus_tag="PKX09_02545"
     CDS             48513..51659
                     /locus_tag="PKX09_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOU16734.1"
                     /translation="MSLSNLAIKRGITFTMIYLIVIGFGIFGLTQLKLDLYPNITIPV
                     VGVMTQYTGVGPEDIENVITRPLEQTLVAVENIDKIISMSSVGNSLVILQFDWDADID
                     QSEIDVRKNIDLIRDYLPPDASNPITISFDPSMTPVMLISARSGQLGMAELRRVIDDE
                     VRPRIERLKGVASAASGGGLERQIKVLLDPQKVAAYGIPAQQIVQKLQMENVQIPAGV
                     VDDEYQEYSVRTYGEYRTVEQIANTVIGNVSGTPIYLKNVANVIDGYKEQSQIVRNNG
                     EDAVMLFIQKQSDANTVQTARAVAKELPKIMELVGKDVKLEIVFDSSEFITESLNNLV
                     STGVQAFILVFLVLLFFLRYIRSSLITAISIPVSIIVTFFAMYLLNMTFNVITMAGLA
                     LAIGMLVDNSIVVLENIFRLKEKGLDIKQASDEGANEVSMAITASTLTTIAIFVPVLF
                     LPGISGILFKDLVIVIVSSLTISLLVALTLIPLLSTQWLKGEKKKSKSKVLRRMDSVI
                     DNFLQGLHDRYVRILDYFLSHKKVFLIGLIVIFVITILMFPFLGGEFLPEADRSSIRI
                     SVERETGASLASTDKTFREIEKIIREEVPEAENIQVNFGVGEGVTAIMASSNSGNITV
                     RLLDVEERERSSFEIQDILRERFQKIPGANITIASGEEATSMLGGGITIKIFGYDRLV
                     ARNLGDQVAEMLKGVKGVVDVTKSYNLPKPEYQIIIDRDKASAFGLSTYQIANIVGTS
                     IRGTRVTRFREGGDEYDVIVQLDEQFRKSQGDIENLYITTPTGAQFPLKNIARVLPGT
                     GEESINREDQERRVSISCSVSGRDIQSVTRDITAGLRNINFPPEFRWEIGGAEEERRE
                     AFLYLYIALAAAVILVYMVMASQFESLIDPFIIIFTIPLAMIGAIWLLFITGTPMSVM
                     ALVGCILLVGIVVNNGIVLVDYINQLRYKHNYHLWIAILAGSKRRLRPVLMTALTTIL
                     GMLPLALGLGTGAELWAPMAIAVIGGLTFATVFTLILIPIIYLFFEQIALKRKIKKGK
                     VEAEPIGRPEGFDINLVK"
     gene            51752..51934
                     /locus_tag="PKX09_02550"
     CDS             51752..51934
                     /locus_tag="PKX09_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16735.1"
                     /translation="MKYCDLTCEYAEFPDKLSDGSATCQTFIAIHCKLLDRLVDKNGI
                     CPAEQSATDKSVNKGR"
     gene            52014..53843
                     /gene="uvrC"
                     /locus_tag="PKX09_02555"
     CDS             52014..53843
                     /gene="uvrC"
                     /locus_tag="PKX09_02555"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOU16736.1"
                     /translation="MAERQFPEKLLEKIRTAPTDPGCYIYRNSAGKIIYIGKAKNIRN
                     RVRSYFIGGYKEPKTAALVNNIADVEYILTNSEIEALILENTLIKTHHPKYNIFFRDD
                     KTYPYVRITNEPFPQVFITRSVVRDGSKYFGPYTDSRLLRDTLEIIKNIFPIRSCKHH
                     LNDKTIANGHIKVCLDYHIKKCEGPCQRLVSAEEYNRMINQVIAFLNGRTEGSIEYFE
                     EKMKTAAASLQFETAAAYRDKISILMNYSNRQSVELNDELDRDLIAVLKEAENCCAVI
                     FRVRQGKLIGRDKFLLEGTENYDYPAITHDLLLRYYDNATSIPSEVLLNYLPEEQEML
                     EKWLMSQKGARVSILQPQRGDKARLMKMAIRNAELQISELQMKQSQKTEFVPKSLVAL
                     QNELRLKELPRRIEAFDISNIGGKFAVGSMITFINGVAKSSEYRRFKIKTVTGSNDYA
                     MLAEVVRRRYTRLLREEKPLPDLILIDGGKGQLNAAVTVLQELNLSAIPVIGLAKRLE
                     EIFIPGNDEPILLARNSLALSLLQRVRNEAHRFAITYHRKIRGKGGISTKLDEIPSLS
                     QNTKKALLQHFKTIANIKSASTEDLMNVKGIGLKIAQKIKQYL"
     gene            53857..55095
                     /gene="lysA"
                     /locus_tag="PKX09_02560"
     CDS             53857..55095
                     /gene="lysA"
                     /locus_tag="PKX09_02560"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOU16737.1"
                     /translation="MKINGLNVSDLADEFGTPLYVYDFDRIKDNAQRLRRAFDSDEIE
                     TAFFYAVKANSHPAIVQLLKDCGYGCDCVSPGEIEIALKTGVKPSDIIFAGNYESMED
                     FNVAVEAGVNIILDDITSLDRLLRIHCPEVISFRINPGKGRGKYEQITTGGEKAKFGV
                     PHEKAWLAYEKARQVGIERYGAQMMTGSGILDEEHFPRMLELLLNILGQIHQKLGITF
                     EYVDMGGGFGIPYYGDDHELDIQYVGRECLKVLKAKSESLSIGKPALHLEPGRYLVGD
                     AGYLVTRVLGIKESYQTFAGLDAGFNTLIRSALYKAQHPVIVDGKEDEKELYPINLCG
                     QICENTDIFTVGRPLPKLEEGDLLIFTQAGAYGSTMSMTYNHRLRPAEVAIINGKAIE
                     ITRRETMSDYWNRITYPILL"
     gene            55122..57356
                     /gene="purL"
                     /locus_tag="PKX09_02565"
     CDS             55122..57356
                     /gene="purL"
                     /locus_tag="PKX09_02565"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOU16738.1"
                     /translation="MASQIEPEVNLQTAIQHGLTEEEWNKVLKILGRTPTYTELGIFS
                     VMWSEHCSYKNSIALLKTLPRNGKRLLVGAGEENAGLVDVGDGLAICFKIESHNHPSA
                     VEPFQGAATGVGGILRDIFTMGARPIASLDSLRFGPLEAPRNRYLFDNVVKGIAHYGN
                     CVGVPTVAGEIYFDNCYTENPLVNVMTIGLVKHKNVISAVAKGEGNTIMIVGSSTGRD
                     GIHGATFASVELTEESENRRSSVQVGDPFTEKLLLEATLELAKQDFLIGIQDLGAAGI
                     ACSTSEMAVRGKSGMIINLDSVPLREPGMSPYEIMLSESQERMLVVIRKGFEEETKAI
                     FEKWDLNCVKIGKVINSGNLDIFMNDKKVASLPAWHLVLGGGAPVYERETKEPAYLKK
                     VQGRNLKKIPEPKDYNKVLLRILSSPNIADKSWVYRQYDYSVRSNTVVKPGGSAAVIR
                     IKGTRKALAIKTDGNGRYTYLNPYKGGAIAVAESARNVVCSGARPIAITNCLNFGNPY
                     DPEIYYQFKMAVQGIGDACRVFDTPVTGGNVSFYNESPKGAIFPTPVIGMVGLIEDLK
                     NVTTPWFKNDGDFIILLGALKGELGGSEYLKVVHNIIAGDAPDIDLTFEKRIFNATLE
                     AIQKGIVNSAIDISDGGLAVALAEACISNPEKPLGASVYISRKLRNDELFFGESQSVI
                     LISINEKYLLDIERIASKNIIPSVTIGRVKDNGRLTINNIIDLPLTELQKAYTRTIPG
                     FMDI"
     gene            complement(57391..58404)
                     /gene="galE"
                     /locus_tag="PKX09_02570"
     CDS             complement(57391..58404)
                     /gene="galE"
                     /locus_tag="PKX09_02570"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOU16739.1"
                     /translation="MKILLTGGAGYIGSHTLVELLNQNHEITVIDNLSNSKIESIKRV
                     KTITGKELIFNKIDLCNIIELEKVFSSNQFEAVIHFAGLKAVGESVQKPLLYYYNNIT
                     GTTNLCQMMIKYDVKNIVFSSSATVYGNPTSVPITEDFPIKPANPYGNTKYIIETILK
                     DIQIANPDWNVIILRYFNPVGAHESGLIGEDPNGIPNNLMPYISQVAVGRLPYLKVYG
                     NDYPTKDGTGIRDYIHVVDLAQGHLKALDKLLTNPGYCVYNLGTGQGYSVLDVITTFE
                     KWSQRKIPYKIVGRRPGDIAECYADPSKANRELDWKAERNLDDMCRDTWNWQKKNPNG
                     YSD"
     gene            complement(58417..59496)
                     /locus_tag="PKX09_02575"
     CDS             complement(58417..59496)
                     /locus_tag="PKX09_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16740.1"
                     /translation="MYKKIILLMLIVVINASGNDYEYYFYNPDIDYGSESIFNPISLF
                     INGSFDILRNGAHNKNIFDQPSKTGFNNVIDNVLHPIDNIRNYGVKNFLNREIFNLEI
                     NKNESQFLPNIGLHLIGNGMQYRKLAEWYDYHEYPNPNLLSIITTISYQLVNEIVENG
                     SYSGTNVDPISDMLIFNPLGILLFSTKAGQEFFSNKIPIYDWSLNPYFNPNNYFLENA
                     GQQYATKIKLKTTDKNALFGYWGVVSTFGISHTINETDAISFSAGAVVAKLTPNEEYD
                     SRFIIPNLDYAVAFFYDRNNSLLGSLTLFGPKFYNLRLEIFPGIIGFKNYKPGFFIGI
                     GEMDNFQFGISFPPIPIGIIGEISN"
     gene            complement(59776..60015)
                     /locus_tag="PKX09_02580"
     CDS             complement(59776..60015)
                     /locus_tag="PKX09_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6429 family protein"
                     /protein_id="HOU16741.1"
                     /translation="MKYDKDKVDNMILALLFLTSSSDQYGTKAWKGFDFEALDRLYQK
                     GLISDPKDKSATVSLSEKGAKLSKELFFKYFVIKD"
     gene            complement(60026..61375)
                     /locus_tag="PKX09_02585"
     CDS             complement(60026..61375)
                     /locus_tag="PKX09_02585"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOU16742.1"
                     /translation="MQKITRKNLTCSNLLKLILILLIIGQFSPILVARDTSRAAYYPD
                     PTQYPDHLILSWSDDPTSTQSVTWRTDTSIKSAYGEIALADASPNFVYQAKRIPAKTE
                     LLNFDGKSANYHSVTFTGLQSNTLYIYRVGSDKRWSEWFQFRTASKKPEPFRFIYLGD
                     GQEDVLSLWSRAIRTAYKNAPDARFIIHGGDLVNNARDYPALNEWFTAASWINATVPI
                     MPAIGNHEYYDGNWGEQFFMPYWRPQFTLPLNGIEELPETNYYFDFQGLRMIVLNSNF
                     ELEKQAQWLEEILKNNPNKWTVVAFHHPIYCGAEGRDYQELRDTWNPIFIKYKVDLIL
                     NGHDHTYARGNNPERLTIRPNDPTGPVYVVSVSGPKLYNINKIRWMDRAAENTQLYQI
                     ITINDNILDYKAYTVTEELYDAFQIIENSKGYKVFFDLQPKNFPERMYKNTLNAPNN"
     gene            complement(61460..62497)
                     /locus_tag="PKX09_02590"
     CDS             complement(61460..62497)
                     /locus_tag="PKX09_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOU16743.1"
                     /translation="MKAVSLTGIRQLTIQQVADPTIVNDTDVLIRVKMVGVCGSDIHY
                     YKNGKIGSQVIQFPFIIGHEAAGVVERVGNKVTRVKIGQRIAIDPTVYCANCDQCRSG
                     RENTCRNLKFLGTPKQLNGALCEYIVLPENCCYPISDKMTFEQAVLSEPLAIAIYSVA
                     RSLLPDNANVAILGVGPIGMSVFCILRIKNVGNIYVTDKIQERLDFAQQLNPGWRGNP
                     DLTNVVSEINKREPLQLDVVYECSGDTTAISQAVQLLKPGGILVLVGIPEMDEISFPI
                     HELRRREITIINVRRQVRCTQKAIDLLESHLVNMNAMATHHFPLEKTAEAFELVANYR
                     DGVMKALISIG"
     gene            complement(62570..63553)
                     /locus_tag="PKX09_02595"
     CDS             complement(62570..63553)
                     /locus_tag="PKX09_02595"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HOU16744.1"
                     /translation="MKQIRIGIVGYGNLGRGVEQAVTQNPDIELVAIFTRRDPAKFTT
                     ASKSTMVLSANELASMQSAIDVMILCGGSATDLPKQGPAFASLFNTVDSFDTHAKIPE
                     YFAKVDKAARDNGHLAIISVGWDPGLFSINRLYAEAILPNGKSYTFWGKGVSQGHSDA
                     IRRIKGVKNAIQYTIPIEESLVAVRSGANPQLTTRQMHRRECFVVLEEDADRDYVENT
                     IKNMPNYFIENETVVHFISEDEFKKNHSKMNHGGFVIRSGHTGAGNRHIIEYSLKLDS
                     NPEFTGNVLVCYARAAYRLAQSGAIGARTAFDIPPALLSPKSGEQLRNELL"
     gene            complement(63573..64277)
                     /locus_tag="PKX09_02600"
     CDS             complement(63573..64277)
                     /locus_tag="PKX09_02600"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HOU16745.1"
                     /translation="MRYRIELLVLIVTFCLFATICSKKQSEPTSEFSPEVGDLMFQDL
                     DGSPFYDAIETVTKGYQGASLSHIGIVTKDSSGKIFVLEAILAGVSLTPLFEFLARSY
                     DNQNHPKVLVSRLKPKYRHQILPALKEAQKLIGKPYDNFFDIENDAYYCSEVIYKAFL
                     KSTDNQSLFKLQPMTFVDPSTGETFPIWQEYFAELGVAIPEGKLGINPGEISRSKAID
                     IIYNYGIPNGWKKLNK"
     gene            complement(64583..>64885)
                     /locus_tag="PKX09_02605"
     CDS             complement(64583..>64885)
                     /locus_tag="PKX09_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU16746.1"
                     /translation="TGTGLKLANSSVAYEFKLEQNYPNPFNPVTVIKYSIPEDQLVTL
                     KIFDMTGKEIDVLINEFQTAGIHEVQWMPKGLPSGIYFYRIQAGAYSETKKLVLQK"
BASE COUNT        20103 a        11783 c        13849 g        19050 t
ORIGIN      
        1 ttattttaat ctggaattgt acgatcaaag cgcaattcag gtttgagaga gaaattgttc
       61 cgcaatcagt cagagaagga gtgcaattta atgccatcag cgtcaatgag ttcgacgata
      121 tttatctact ggaaagcaaa ttcagtgaag tttatcgtct cgaccaaaat ggcaatattc
      181 tgaaccgcaa cggcggtttt ggatggggat tgggacaatt cgattgccca cacgatttgt
      241 gtttttctgg attagatgta attgttgccg accagaataa tcaccgcttg gtgcggtatg
      301 atcggcaatt gaattacatt gcgactcagg atttacgcag tgattctcgc cgcttaattt
      361 atcctatgtc tttgacagcc tcgcagatta atgaactctt cattttatcc ggtgaaacgg
      421 ctgagatttt gcgcctttat gtcgaaaaaa atgaacaaac ctggttcggt gggatcgaat
      481 atggtgtcta tgcgctggtc catccggtaa gtctgcggat aaatcgcaaa ggtattttaa
      541 ctgtgctaca ggaagacggc agtttggtgc aattcgaccg ttttgggaca ccgctcggat
      601 taattccatt ccataattca aaaataaaca aatgtaaagc ttatggatta acagccgtca
      661 ataacgactg gctgattctg attgaccaga ctccgttttt gcgaatgttt tatacgaaat
      721 cgaaaatctg ggacaagccg actttggctg gttttgatcg tctggagcct tttatagcca
      781 cgactttttc caataatcgg ctttatttgt tgaccagtaa ttgtacaatt ttagtttttt
      841 cgatggaaac ttctcagtcg aaaccttagt gattagctgg tggttaaaat ataacttttg
      901 aatcacgcca gttcattata tcgaaaagca tataaacgat ggacgattac ctctcgtctg
      961 gtaggaattt tatttatatt cacgagactt cttattgctc agcagtggca ggtggatact
     1021 ttgaagattg cggcgagcga ttcggttgtc tatttatctc atagatttgt catacctgaa
     1081 acaattgaat tgaacgttac tgattcaatc agtctcgatc ctttgaccgg tgctattact
     1141 ttacattcta caagaactga ttcagcgttt tttattgcca aatatcaata tttgccgctc
     1201 aatttgccgc taaatagaat agttaacctg ccgccgcgca tttatacagg taatgaaatt
     1261 gccgaaccca gttcaactga ttatccggcg catccgcccg ctcagtattc taccgagacc
     1321 accgatttcc tgaagtccgg tacgctttac cgcggtgtga cccttggtag tcagagtgga
     1381 atgtcgcttc agtctggctt gaacctggag ttgcaaggga aaattgcgga ggatatttcg
     1441 attgtcggca caattaccga ccagaatata ccgattgaac cggaaggcaa tacgcaaacc
     1501 cttgatgaaa ttgataaagt gttcattacc gttgtcttgc cgcatgaacg actgactttt
     1561 ggtgattatg aaatggctta tagctccggc agtttgggaa attacaatcg caaattgcag
     1621 ggcgtttatc tggaaagcga gcgaccgccg atgcgcaatg ttatgactgg agcagttacc
     1681 aaaggtcaat attatcataa ttattttctc ggagaggaga gcaatcaagg tccgtatcag
     1741 ctgatcggca gaaacggcga aaccgatata atcgttctgg ctggaacgga gaaggtctgg
     1801 ctggacggtc gccttttaac ccgcggcgaa agcaatgatt atatcattga ttattcgacc
     1861 gccgagataa cttttatgac tcgtcaaatt attacagcgg aatcgcgcat tagtgttgat
     1921 tttcaatatt ccgatttgat ttaccagaaa aatatttata tggcaggcaa tactacgaac
     1981 ttatttgatg agaaattaag agtttccgcc gccgcgatca ctgaaaaaga tgaccgcgag
     2041 aaccccatcg aacttgttat tagcgatgcg gatagaaaat tgctaaaatc gctcggtgac
     2101 aacgctcaag acgcttacat aagtaccatt acgagcgata caaacggctc ttatatttta
     2161 gtagattcaa tcttgattta tgctggtccc ggtcagggaa ctcatacagc gacgttttat
     2221 aatgtgggaa aggctggcag ttataagaaa gtttaccgtg gcgatgtaac atattttgaa
     2281 tgggttgaca aaaataatcc cttgacggcg gcttccgata ttgccgcggc gctttatttg
     2341 ccagtagaac cgttgaaatt gccgaccagt cggacgcttt atcatttggc aagtgactgg
     2401 caaccgcatc aaaactttgc gataaaggct gaagtggcac gttcagattt cgatcaaaac
     2461 ctattctcac ctctggatga tgaggataat gccggaatag cagttgatct tacgtcaaat
     2521 ctaatcattc cgctttccga atccagtaag attgctctca ctggtcatta taaacaggaa
     2581 aattgggatt tcgagccgat tgatcgtcat caaatagttg agtatcgaag gaaatgggac
     2641 ttgccttctg attcgactaa cggtgaacag tattatgaag ggcaaattca atacgctttg
     2701 aaaaataatt tagtggtcaa aacggaaggc ggtcagtatt cgcgtggtga ttttaattcc
     2761 aatagattag ctgccagtgc cggtttgagc tataaaatgc tggaaactgt ccaaatcagc
     2821 gaagaattca ttcaacgtaa tcaaaccggt tttaatgata ttagttggac gcgtcgagga
     2881 ttcaggttaa taacccattt atggaaagtc aaaccgtttg ctacactaaa ttacgaatta
     2941 cgagaaggtg acaccattag aactgaaaat ttccgctttc tggaacaaat ctatggaatc
     3001 ggctcggcga gtccaggatg tctaacttgg caagttcaga cgactctccg tcgggatgac
     3061 ctttcagatt caaatacctg gataaaaggg acaaacgctc gcaatatcgg tctgaacggc
     3121 caattgataa actggcattc attctcatcc cagtggaatt atatctaccg tataaagaaa
     3181 ttctataatc agaataactc agatattgtt gttcatctcc tcgatttctc cctaaagcaa
     3241 gaaccacgca aattacctta tcgctgggaa accactctga aaattgaaaa cgagcagact
     3301 gtcaaaaagg aatggcgcta ctattatgtc ggcgatggaa tgggtgatta catttatgat
     3361 tcgacttatg ccgattacgt tcccgatccg caaggcgact acattttacg gattcttcct
     3421 tcttcgatca aagtgccgat caccagtatc gaggatggat tgcgttttca atttgacggt
     3481 gccaatgtta aaatgccgat tttcggtgat tggctaaaac gtatttctac cttgactgat
     3541 gtccgtctcc ggcaggaaat ccacgatccg cagggaataa taaaatacct tgcgacggat
     3601 attaatcagg ttgataccag ctgggcaaat tattcccgca taattcaaca agatgttaat
     3661 ctgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngttgat accagctggg
     3781 caaattattc ccgcataatt caacaagatg ttaatctgcg ttcgcgagaa agaaaaagcg
     3841 atttgcgcct tcggttttat tcctccgatt tgataactca ggctgatgtt cggggattag
     3901 aaaaaacatt caccaacgaa tggtctctgc gttatcgcaa tcgttattta ggaaaaacca
     3961 caatcgagtc cgaatcggca ctaaaaaatt atagtcgtca atcggagata aataatctta
     4021 gggaacgtga tatttatact gttcgggaaa agttaaccat atcaactttt ctggatcgga
     4081 ttcatctaat cggtacagaa ataacgctta acagtgacaa agcgaaagga agtgacccaa
     4141 ttgagtcgct cctagctggt gtaaaattga gttatgaacg aaaaattgtc gggaaaggtc
     4201 gctggaaatc atttgttgaa ctggataaag tatctgtaac ccctaaaggg aaagcaattc
     4261 cgtacgagat gagcaatggc aagaaggaag gacttaccgg tggttggggg acaactgtcg
     4321 agtaccgcgt cggttcgaat gttagtatac gagttaatta cgaaggttgg aaagagcctg
     4381 agcgaaccgt atatcattta ggaagtggcg aagtgaggct ttcgttttaa tgacagacca
     4441 gcggcatagg cgaaaaaatt tacagattgt catcttcttg ataatcggat ttgtcggttt
     4501 tctaccgctt ttgggaattg ctcaggacac ttcacgagct gaagtgattt tcggagaaat
     4561 tacggttagg gcgcttgagg aaaagttgac tgattttact gaaattacca gggattatac
     4621 taattatccg gttaccgctg attcgtatga ggagttgacg aataaaatac tggatgccgt
     4681 gctaaaggat ggctaccttt acccagtatt gactttaagt gcggttcagc ctgaaactga
     4741 aggaaatctg accaaactga acccagaatt tcgattggat cgcggtgagc gggttgtcat
     4801 tgatacggtg atatatgagg gagtcaagcg caccgctcct gaattattaa agcgcgaatc
     4861 ggcggcaatc cttggtaaat cacccgattt gcaaaaaatc tcagcctttc tgaataattt
     4921 taagagttat tcatttctga cggtgcaatc tcaaccggaa atagtaaaaa cccgcgaagg
     4981 tcaatttggc ttagttgttc aactgactga acattcgact aataccttca gtggaatagt
     5041 cggctacata ccagccactg ctacgaccga aggttatttt accggtgaga tgaatcttaa
     5101 tttgaataat atcagcgggc gcggtcgcag tctgagcgta ttttgggcga aacccaaccg
     5161 ctattctcaa caaattgacc tgagatattt cgagccgtgg attcggcaca ccaattttaa
     5221 tgcggaaggc caattcagtc agatattgcg agatactctg gtggttttac gttcttttga
     5281 agccggtatc ggccgcagat tattccagaa cggctctggg gctttgaaat tcaattacac
     5341 atctacaatg ccgacctcag gcggtcgcca gttactcgga ttaaatgatc tcacgactcg
     5401 cggtttggga ttatcattcc gctatgatgg tcgcgattat ccacttaatc cccaaaaggg
     5461 ggtaatggtt gcgattaatg cctttctcag tcgtcgtatc gaacagaatg tcggtcgcct
     5521 ctggcaatat cagactgaaa taaatggtga gttggatgtg aaaattcggt cccaattagt
     5581 agccgcctgt ggctggaatc tcagagggaa gtggctatcg gaaggcgtgc cggtttattc
     5641 cgactactac tggtttggcg gttctcgcag tctgcgtggc tatgctactg agctgtttgg
     5701 tggctcggaa gttgcctggc tcaataatga gattcgctgg cttatcggta aaaacggtcg
     5761 ggcgcatata ttcttcgatc agggatatta tcgccgttcg gttgaaggct tagataaaaa
     5821 gtattttcct cattcctatg gcgtcggctt gcgtttggat tcgcggatgg gagttatcgg
     5881 gctcgattac ggattcggag aaggcgatac ttttacaacc gccaaaattc atatattttt
     5941 ggagagtaaa ttttaatggg aaaacacaag aataacagtc caaaaccgaa accggtgaaa
     6001 gttagaacgg atacgcaaat tgtcgagcgg attattcaat ttcttcaaag aaataaaggc
     6061 aatatttata aacagaaaaa aattgctaga gaagtcggca tctcttccaa taagtatgtt
     6121 agattcaaac aaatattgaa agaattagca atagaaggag aaattgaaaa tccgcggtcc
     6181 ggttggtatc gaattcccga taaagcgcgg tttgtggaag gaataatttc gttctccgga
     6241 cgcggttttg cttttgtaaa tactgacgcg ggcgaagaaa tcttcatcgg cgctgatcat
     6301 accgctaatg cgctacacca tgaccgggtg ctaatcgaaa aattgcggta tagcaatgga
     6361 attcggccgg aaggtaaagt ggtaaaagtt ttaaatcgtg gtcaaaaatc aattatagga
     6421 acaatgaagc ggaaaaataa gcactgggtc gtgattccgg aacatccggc cgctgttgag
     6481 gagatcgaat tagtaggttc ggaaaaaggt ctgcgggaag gtcaattagt cgaaatccag
     6541 gacatcgagt ggagcaacgc tcgcctgcga ccgcgagcca ctttaaaaca aattcttggt
     6601 tttccggaga atccgcagga tgatttgatc atcgtaaaaa aaatgtatcg gctgagcgac
     6661 cgatttccga ataaagcttg gcaggaagcc aaccgcctcg gaaatcagaa aataaaagca
     6721 gacaatcgtc tcgatttgcg tgataagttt atttttacga tcgatcctcc tgacgcaaaa
     6781 gactatgatg atgctgtatc gctggagaaa agtccagatg gtaattggct tctgggtgtg
     6841 catatagcag atgttagtca ttatgtgacg ccgggcagtg tactcgatcg cgaggctcgg
     6901 cggcggggta ccagtattta cctgggcgaa aatgttattc caatgttgcc gccgccaatt
     6961 tctgatgagt tgtgtagtct gaaaccggga gaagatcggt tggcattttc ggttatcttg
     7021 acacttaacg gtgcaggtgc ggtggtgcgc tattcatttg ctccgagtat aattcgctcg
     7081 tttaggcgat ttagctacga agaagtgcag gagattctga ataccaaaag cggtgaatat
     7141 tttgatatgt ttaccaatat gcgtcagatt agtcgcactc tttttgagcg ccgacgggcg
     7201 gcgggcagta tagacttcga cattccggag ccagttttta agatgggacc ggctgggatt
     7261 ccgattgaaa tcaatccttc tgaaagattg gacagtcacc gaataataga ggaatttatg
     7321 ctggcggcta atcgggcagt cgctgaatat atatctatta aacggcgcaa tgaaaaactg
     7381 ccttttctct atcgaattca tccccaaccg ccgcaacaga atatcactga attgtatcag
     7441 attttgagaa atttggggct cgatttgcac cagcctccca agctcaaacc gatggatatt
     7501 cagaaaattc taaatggatt gactgaaatg ccctataaaa attttattga acaaattaca
     7561 ctgcgttcga tggctaaagc aatctattct catcaaccgt taagtcactt cggtttggcg
     7621 tttcctcatt atacccattt caccgcgcct ataagacgtt atcccgatct ggtggtacat
     7681 cgtttgctga aaaactattt aggtgattat gattctaacg acctggcttt ttaccggcgt
     7741 tcattaccgc acattgccag ttctgccagc caacgtgaaa tcgaggcgat ggaagccgag
     7801 cggacttacc agaaaatcaa gcaggtgcgt tttatggcgg acaagatcgg tcgatggtac
     7861 aaaggcgtca ttagtggcgt gcgtgaattc ggattttttg tggaaatttc ccaatttctg
     7921 gtcgagggat tggtacacat tcggactctg agtgatgatt attatattta cgatccggaa
     7981 aatcatcttc tacgtggtaa atatagtcaa cgcatttttc ggcttggcga tgaagtgatg
     8041 gtaatggtga cggatgtttc agttagagaa cgacggatag atttggagtg gtgtgaataa
     8101 ttaaaagatt tattggctta ataccaatga atgtttaatt ttaaagttga ataattgatg
     8161 gggtataaaa atgaataaaa aaattggatt attagttttg attatttccg ggatgttgat
     8221 gaattgcaac cagctgcggg aatctatcgg cggcgttgac gatatttata tttttgctac
     8281 cgatcaaact cgagaagtgc tgggtaaggc aattgatacg actttttcct acggaataag
     8341 gacgccggaa tttcaaccct atttttcaac aaaatggttg ccggtagaag aatttccatc
     8401 atatatcaaa tttaaaaaca tcattttggt agccgattta actcaagatg acctggcggc
     8461 tgaaattgcc cgtggtatat taccaaagca aagtttaata gccgcggaaa aggatagcat
     8521 tcatatgttt gccattccgg ataactgggc tcgagatcag atgttcgttt taattgccgg
     8581 ccgcaatttg aagatggttg aacgcagtat cttggaacaa aaaggctggc tttatggaaa
     8641 gtttgaagag aaattcgaag aagtccagat ggattatata tatggacgct atgagcaaaa
     8701 aaaactaact aattatctct ggcagaaata tcgttggacg atgcgcattc cgcacgatta
     8761 tatcattatc agagaattgc ccgaaaataa cttcgtttgg ctcggaagag gtttgccgta
     8821 ccgttggatt tcggttacat ggggagatgg cattcagacg gaatggttaa cgaatgtggg
     8881 actatacgaa aaacgcaatg agattggcaa attatatggc gggattatga ctgacaagcg
     8941 ctttctcgga tttcagttct tcaaatttgg tgaatatgat gctttgaaaa tgtatggact
     9001 ctggtaccat actgaggaaa cgcaaggtgg tccatttgaa acgtatgcct tttatgactg
     9061 gcgcaccgac cgaaccttta ttattgacat gctaatgtat ggtcctggtg aaaaattgtc
     9121 agtcctattt cgcgggctcg aacttaccgc taagacattt accaccgatt acaaaggctc
     9181 ctggaaatag agctagaact ggtcggtagt cggaatttgg caattatagg gtggtcaatt
     9241 aaattacggc ggagcaagta gatttatgga gaaaatagca ttaattgtcg gaagcaattc
     9301 tgatttagaa acagtcgaac aagcgcgcaa gtatctggaa tattttgaat tagactgcga
     9361 aataaaagtc ctttcggctc atcgccagca cgacgaattg gtaaattttg taaagcggtt
     9421 tgagaaagaa aacggccgtt tgattatcgc ctgtgctggg atggcggcgc atttgccggg
     9481 tattatagcg gctctgacta ctttgccggt aattggcgtc cctttgaatg cttcgtcttt
     9541 gaacggggta gatgccctct attccattgt gcaaatgccg ggtggtattc cggtgggcac
     9601 gatggcgatc ggcaaacccg gaatcattaa tgccattatc tatgcggctc gaattttggc
     9661 gctctccgat gccaaattaa aggctaaatt ggtcgaattc gcggcggcag gcttcaaact
     9721 caactaataa tgaaaatatt aataaccggc gctaacggaa tgttaggtga aaaatgcact
     9781 cgtttgctag cagtaaaaca tagagtttta gcgactgacc tccatgataa actgatttac
     9841 aaattagaaa ctgactaccg caaaatggat atcacaaagc tggaagaaat cgaaaatata
     9901 atcagtgaat ttcaaccgga ttgggttgtc aattgtgcgg cttatacaaa tgtagatggc
     9961 gctgaaaccg accgcgaaac cgcctggcaa gttaatgcag tcggtccaat gaatcttgta
    10021 gccgctttac gaaaatctaa tactccgatt attcagattt caaccgatta tatttttgat
    10081 ggcgtcaatg gaccgtatag agaaaatgat ccggtaaaac ctattaatta ttatggcgaa
    10141 accaaattgg ctggcgaacg cgctgtttta gaatcagaat taccagcaac agttttccgg
    10201 acaaatgtcc tttttggtaa ctcgactaat caggaagcca gctttgttta ttgggtggtg
    10261 aataaactca gccaacggga accgataaat gtggtcaatg atcaattcgg taatcctacc
    10321 tgggctgacg gcttggcgca agtgatcgaa atggcgattg agcggggaat tcacgggctt
    10381 tatcactatg gcggggctga ttatatcaat aggctggaat ttgctttgca aattgcggcg
    10441 gtttttgaac tggatccgac tttgattcgg gctgtttcta ctcgttcgct gaatcagaaa
    10501 gcccctcgac cttttcgagc gggattaata tgtgaaaaaa ttcaatctga atttgggatt
    10561 catttatatt cagttaaaga agctctaaca aatatgaaag aaaaacaatt tgaagtcact
    10621 agtaatcata ccaacatata acgaaaaaga caatattcgc accgttatcg aacgtttaca
    10681 ggctttaccg atcgagctgg atattttaat tattgacgat aatagtcctg atggaaccgc
    10741 cgatattgta agggaattac aagccgatga tcctcatatt tttatgataa atagacccgc
    10801 taaactcggt ttagggacgg cttatatcac tggtttccgc tgggcacttg aacgtgatta
    10861 tgaatatatt ttggagatgg atgctgattt gtcgcataat ccggacgatg tgcccagact
    10921 cattcaagaa tgcgagaacg gttatgatct ggtaatcgga tcgcgctatt gtaatggagt
    10981 gaatgtcatt aattggccta tcaagcgttt gctcctgagt tatggggcta ataaatatac
    11041 tcgtatgatt actcgaatgc cgattatgga tgcaaccgcg ggttttaaat gctaccggcg
    11101 ggaagcctta gccgcgatta atttagatcg cgtaaaatcc tccggctatt cctttcaaat
    11161 tgaaatgcac ttccgggttt gggatgcggg ttttaaaatc aaggaaattc caattatttt
    11221 tatcgagcgt tccgaaggct tgtcgaaaat gtcaaaaaat attgtttggg aagctgtatt
    11281 tatggtctgg aaattgaaat tacggcagat tttcagatct ttgtaaacat gcagatctcg
    11341 gtcatcattg ttagctacaa cgttaaagaa tttcttcaac agtgcctttt gtctttgaag
    11401 aatgcccttg agggtttatc aagtgaaatc atcgtagttg ataatgattc ggttgacggt
    11461 actgttaatg tcataaaaaa taaattcccc gaaatcatat tgatagaaaa ccattttaac
    11521 aaaggatttg cggcggcatg caatcaagga ttggcaatcg cgcgtggcga atatattttg
    11581 ttattaaatc cggatacgat gcttcaggaa gatacaatta aagtaatgat cgaatttttt
    11641 agggctcacc cagaggctgg agccgccgga tgtaagatac tgaacgctga cggttcactc
    11701 caactggctt gtcgccgctc ttttccaact ttgacagtgg cgttaccgaa attacttggg
    11761 ttgagccgtc tatttccaaa agtgaaggct ttttcgcgtt ataatcttac ctatctggat
    11821 cccgatcaaa tcaccgaagt tgatgcggtt agcggttcat ttttaatgat gaggagagcc
    11881 gtatatgaac agattggcgg attggatgaa tcctattttc tttatggtga ggatttagac
    11941 tactgctatc gaatcaaaaa aagcggctgg aaaatttatt atgtgccgga tacgaaaatt
    12001 attcattata aaggcgaaag tgccaaacat gccgccttcg ataattttat cgcattttac
    12061 cgagcaatgg ctattttcgc gagcaaacat ctgggaaaaa gttcctcgat aatcactgat
    12121 attgttttca aagtcggtat ttttttccgc gggataatat cactggtagc gaaatttttc
    12181 cgacgccgaa tggtaatgat tgtagacggg atagctttgg tagcggccat tttggtcgct
    12241 catcaattac aacccaagcc tctccctgat tatccttcgc tactatcaat gttggttttt
    12301 tatttactgg tttggttggg aataggatat tctattggct tatatgatcg tcgggaatta
    12361 tcctattcac gagcagttgt tgcttccttg cttagtttcg ttgcttctct gatctttaat
    12421 ctgattttta aggacgtcat ttattcgccg aatatgctgt tattgagttt tatcattgtc
    12481 actctgtttt taccaggctg gcgggtgtta ttattatttt tacagcgacg tcgtgtaatt
    12541 caaccggcat catcattgtc aaaagctctg ctgtcccggc gaactattat cgtcggagcc
    12601 ggtaaagaag gtgaacgaat tgcccgtaag ttaaaaactc atatagaaca tggttttgaa
    12661 attttgggtt ttgttgataa agattatatg aatgaggaaa tcgccggatt tccttttctg
    12721 ggattggtaa gcgatttacc tgaaatcatc cggattaatc atgctactga aattatcttt
    12781 actaccgatc gcttcaataa cgatgatatt ctggggattg tagataaaat caaaaagtac
    12841 agaactaata ttaaaatcgt tccgcgcaat ctggattaca ttttagggaa atcgagtgtt
    12901 gaaaatattg aagacatacc actggtaaat atcgactata acttgtttca tgtcggcaat
    12961 cgggttacca agcggatttt tgacctgtta atttcttcaa taagtataat tttgctatct
    13021 ccgttcgtta ttccgatagc gtttttgctg ggttatagat tccagaaagc caattttatc
    13081 ggggctgaac aacatcagat cagtgcgctg actttggtaa aaagaaatgg taatcatgtc
    13141 catccattcc taaatcgttt cccattactg ttttcagttg taaaaggcga tatcagttta
    13201 gttgggagtg agttggtacc agccgaaaat ggggaacgga atttgcgttt taaaccggga
    13261 ctgaccagtc ttttccgggt aagtaaaaat catcgcaccg acgaaaacga caagcagaat
    13321 tacgaacatt tttatatgca aaaccattct ttgtttctgg atgttgagat tattttgaaa
    13381 acggttttaa aaatttgaaa aaataatggc tgaaatagtt cttgattttg aaaaaccaat
    13441 aatcgagttg gagaaaaaaa tagaggaact gcgagcacaa agtgcggcca gcggggtgga
    13501 cctcaatccg gaaattgaaa aagccaagct caaactcaac cgcctgattc aaaataccta
    13561 ctctaattta actcgctggc agagagttca acttgcccgt catccagagc gtccatatac
    13621 cctggattat gtcaaaagaa tttgttccga attcattgaa attcacggcg atcgatatta
    13681 tgccgatgat aaagccgttg tagccggatt ggcgcaaatt gaagattata aagtcatttt
    13741 gattggtcag cagaaagggc gaaccactaa ggaaaacctt taccggaatt ttggtatgcc
    13801 gcatccagaa gggtatcgca aagcccgccg ggctatgaaa cttgccgaaa aatttaaacg
    13861 tccggtaatt tccttcattg atacgcaagg agcgtttccg ggagttggtg cagaagagcg
    13921 tggacaggca gaagcgattg cccgtaactt atttgagatg gctcgcttgc gcactccgat
    13981 aattgtggta gtaatcggcg aaggtgccag cggtggcgcg ctcggcatcg gggtaggcga
    14041 tcgcatcctt ttactggaaa atacctggta ttcggtcatt actccggaag gttgtgcttc
    14101 gatcttatat cgagatagct ccaaggcgcc gttagccgcc gaggcaatga aggtaaccgc
    14161 ccgtgatctt gccgaattta aaatcgccga tgaaattatt ccggagccac ttggcggagc
    14221 ccatcgtaat cccgatcaga tggcgcagac tctgaaagat gtcttgctca ggtatttggc
    14281 tgaattaacg cagattccga tagatcaatt agttgatcag cgaattgaaa aatatgcccg
    14341 tatgggttcg tggaaagtgg ttgacgttaa atccaaatcc aaataaaaaa tttgataggc
    14401 ttttaatatg ttagaacacc attaccagtt gcgagttcgt tactcgagtg ttgatcaatt
    14461 gggagttgtt tattattcac gctatctgga atacttcgaa gccgcccgcg atgagataat
    14521 gcgagccatt ggactcccgt atagtcaatt tgaacaggct ggttatgcga tgccggtagt
    14581 ggaagcccat tgcgtttatc atcgaagtgc gcattttgat gatttgctaa caattatttc
    14641 tcgcatttcc gcaattccaa cagcccggct tcgaattgat tacgaaattc tacgtgaaaa
    14701 tgaatctcag cctctcgtta gcggctatac gattcacgcc tttgttaatc aatccggaaa
    14761 agcagtccgt ccgccccaat ttttcttaaa attattagcc gcgcaaatgt caaaatagag
    14821 tttccagtat tttagtaaaa ttcaatagaa aagtattcat taatgactaa aggtctgcta
    14881 atcacattcg aagggattga cggttcgggc aagacgaccc agattaaata tttcataaaa
    14941 aagtgtgagc gagaaaacat tccagtggcg ttatttcggg aaccgggcgg tacgccgatc
    15001 ggtgaaaaaa tccgcgaaat tcttttaagt aaatccaact cagaaatgtt tgccattact
    15061 gaacttttac tttactcagc cagccgacac cagctttgtc gggaattgat tcgcccggct
    15121 ttggaatcgg gaaaaatcgt tgtctgcgat cggttttacg attcgacgac tgcttatcaa
    15181 ggctatggta ggggaataga cctaaatttt ataaatatgc tcaatcgcat cgccgctgat
    15241 aatctggtac ccgatttgac ttttatcttc gatattccgg ttgcggaaag gcttcagcgc
    15301 cttggtacgc gtgatcttga tcgtctcgaa agggaagaat ataattttca ggaaaaaatc
    15361 cgacagggat ttttgaaaat cgccgccgct gaacccaatc gtgtttgttt aattgatggc
    15421 actaaaacgg aagagttggt ggctgatgaa gtttggcata aattcaataa aattattaga
    15481 aagatgcaac tatgaaatta tcatttaaaa aaggtttaaa aatctggatt gccgtgctgg
    15541 tttcagtaag tgtcttattg gcttttacgg cgaaggatat ttataaaacg atttcggatt
    15601 cttacaagct gtataatgcg gtctttcagc aaatattgct gaattatgcc gatccactcg
    15661 atgccgccgc tttaacggaa agtaatattc ggaatatgac ccgtgagctc gatccctata
    15721 cggtttatat gaccgaagag gaaaaagagc cgctggaaat tttatcgaaa ggagaatatg
    15781 gcggggtcgg cttacgaatt agtctgcgca atgacacgct gactgtaatt tcaccgatcg
    15841 aaggcagtcc cgctaaaaaa gcgaacattt tgcctggtga tcaaatttta aaaattgata
    15901 caatctggac aaccggtctc gacctcgata aatgcacccg cctgattcgc ggcaatgtcg
    15961 gtaccaaggt cgaattagtg attcggcgtc cgggggctga tgaaggaaaa gtatatattt
    16021 tagagcgggc aaaaattcag gtttatgatg tttattatgc cgattattta gaagaaggca
    16081 tcggttatat tcgattggct gaattttcta aagggtcaac ggcggaagta cgccaggcga
    16141 ttgaaaattt taaagatcat gaccagctta aagcccttat cttagattta cgcggcaatc
    16201 cggggggatt attaacggaa gcattggcgg tagccgagtt gtttactcag ccgggagaca
    16261 ctttgttatt tacgcgtggt caaaatgaga gctccaatcg agttgatgtt gcccgtcgcc
    16321 gaccactctt ggatgagtcc gtaaaaatag ccgttttaat tgacggcggc agtgcctccg
    16381 caagtgaaat tgttgccggt ataattcaag atctcgaccg cggaatcgtt ctgggtactc
    16441 ccagttttgg taaaggttta gtacagacag tttttcgcgt tgataaagag cattctctca
    16501 agataaccac cgccaaatat tatcttccca gcgggcgatt aattcaaaag cctgattata
    16561 ttaaaaatcc aaagttaatc gccgccagtc cgtctcagga ttcgatcttt tattcacgcc
    16621 ataagcgtca gctaaaaagc aagggtggaa ttaccccgga tattattatt aaaagcgatg
    16681 agcagagcga ttttataacc gaactctggc gtcaaaatca gttttattca tttgccgtta
    16741 agtataaaac tgaacataat gaattaccag aagaggttaa cgagttgatc atcaaagaat
    16801 tcaaaaatta tattgatcat tcgggatttc aatttatcga aaaaaccgaa aagaacatcc
    16861 gtgagatgga taagataatt agccaagatg aacgcttaaa gaacgtcaaa attgatttcc
    16921 agccggttta tgaaatattt gattcattaa aagaagccga attcgaaaat aatcacctga
    16981 tgatccagcg tggaattgag aatcagttcg cgatgctcag ggatggaatc gccggacaac
    17041 ttaaagccga tttaaaatat gacaaggtcg tttccgaggc aattttagcg gtcagggatg
    17101 acggaaaata taaaacgaca cttggtttag ccaggtaaag gtttttttgc gaactaccat
    17161 tcaactgttt gccttaatgc tcttcatttt tagtattggt aaaatttgcc aggcagatac
    17221 aacagaagtc aagcaacaaa ttgaaacgat cattgcagaa attcgcaaga aatattgtcc
    17281 ggatcaacga gtcgcgctat ggaaaattga aattgattat caggataatg attataatct
    17341 agttggggaa accgaccatc ccgaagcagt ccgggcttta actaatcaaa taaaacaaac
    17401 tttgccagct attaaaattc aaaatcttat taaaatattg cccgatgaaa cggtgaacgg
    17461 aaagaatttt ggcattgtga ttaattcggt tgagcctttg cggagtggac catcggtatt
    17521 taacgatatg gtaacacaaa ccttacgggg attgatggtc gaaatattga aaagtaccga
    17581 aggctgcttt ttggtgaaga ccgatgatgg ttacctggga tggatggagg ctgaccgatt
    17641 agtagttggc ggcgactcac taaagaccgc ctgggaaaat acaccgaaag tcgtttacga
    17701 tgatttcgag ggcatcattt actctaaggc tgaccaaaaa tctatgccgg tagccgatgt
    17761 tgtcctcggg aatagattta aaatgattga caagcaaggg caatggactc gcgtcgaaat
    17821 tcctgacggt cgccggggtt ttattccgtc ccagcagttg gttagcgaat cggaatatct
    17881 tgcaagaact ccgactgcgg aggggattat aacgactgct aagaatttac ttgggaggcc
    17941 ttatctctgg ggtgccaatt cgcccaaagc ttttgattgc agtggtttta cccaaacggt
    18001 ttatcgtcgc aacggttatc tattagccag ggatgccaac atgcaggtaa atcagggatt
    18061 tgcggttgac acgaccaatt tactggagaa tttaatgccc ggcgatttgt tgtttttcgg
    18121 cccaaatccg gagcaaatta atcacgttac catttatatt ggggactatg agtatattca
    18181 ttgttcggct tgggtaagga ttaatagtct caaacccggc gcggctaatt ataatgatta
    18241 tttgctgaag aaattgcgct atgtacggcg aatcattggc aaataacttt aataaatata
    18301 aatagtaaaa aataattgga attacaggaa tatttttaat ttctgaaagc gcttaagaga
    18361 atttgtcttt tgttgatttc agtgatataa tttagtcttc gatcgtaccc ccgcgaatgg
    18421 accaattata ctatctatat tttctactgt ctcgtttatt tcaatttacc acattttcga
    18481 ccgctaagga ttttacgata tcgaaatcaa atttgtttaa attgtcttac tctcattaaa
    18541 aaaaaagttc gtaaaatttt tcttctgctt aattatattt ttgttatata ttatttttgt
    18601 gatcggtaat tttatataaa attgtaacta caagaacttt aaatggtatt tttgattcag
    18661 taattcagta aaagtaaaat tattcattag taaaggaatt taaaatgagg gtaataattc
    18721 acaataatta tgagaaactg agtgattggg tagcccgata tgttgcggct aaaattattc
    18781 atgccaatca gactagcgct aaaccttttg ttctcggttt accaactggt tcttcgccaa
    18841 tcggtactta ccaggcgctg gtaagattgt ataaagaagg taaagtatca tttaaaaatg
    18901 tcattacttt taatatggat gagtatgtag gactttcgga agatgacccg gaaagttatc
    18961 attattttat gcggcattat ttatttaatc acattgatat tccgcctgaa aatattaata
    19021 tactggatgg aaatgccaaa gatcttgaaa aagaatgtga agaatacgaa gccaaaatca
    19081 aaagagtcgg cggcattgat ttttttctgg ggggaatcgg ggtgaatgga catattgctt
    19141 tcaacgaacc gggttcttca atttcttcac ggactcgaat aaagacgctg tcctatgata
    19201 cgattgttgc caattcgcgc tttttcggtg gtgaccttag ccgagtacct aagacggctt
    19261 taaccgtggg ggtcggaacg gtgctggacg cccgagaagt aatggtgata gcctccggat
    19321 acacgaaagc gcgagccttg cagaaagtca tcgaagaagg tgtcaaccat atgtggacgg
    19381 tttcgatgtt acagttgcat ccacatggca ctattgtttg tgatgatgaa gccacgctcg
    19441 aattaaaagt cggtaccgta gaatatttca aagatatcga ggatgaggct atcaataacc
    19501 tgccggttta gtaggaaagt taggcaatgg aaaatcgcca attaactcaa acagaaatta
    19561 accttcttga aaaacaaggc tgtcgggcag aaaactggga aaaaatcacg gttaagcccg
    19621 attttaatcc tgctaatgtc cgtaatgtta cattcgccgg agcagtagaa ataggtaatt
    19681 gttgtggtgt tcaggaagtc aaaccgggct gtctgaaaaa ttgtggcatt tatgatagct
    19741 atatccgcaa ttgtacgatt gccgataatg tctatatcgc taacgtaaaa aatttaatca
    19801 attatacaat cgagaaggat gtcataattg aaaacgttgg tgatctgatt gtcactggcg
    19861 aaacgacttt cggcaatggt accgagctgg aaattctcaa tgagggtggc gggcgcattt
    19921 tacccattta tgatcgtttg aatgcccaaa tagcctatat gatcgtcgtt tacaagcacg
    19981 atccggcttt gcagaaggca ctgaaaaagt tggtaagcga ttatgccgcc gagcgcaaat
    20041 caagccgcgg tgtaatttct caaggagtca gaatttacaa cagtaatatt atccgcaacg
    20101 tgcaaattgg ggaaaaagct actgtttcag gagcattact actcgaagat ggaaccattg
    20161 ccggttctga ggtggatccg gtttacattg gagaaggtgt aatcgctaaa cattttatta
    20221 tccaaaccgg agcgcgagtt gacagttcag cagtgcttga caaatgtttt gttggtcagg
    20281 gaacgcgtct tggtaagcaa ttttcagctg agaattcggc tttttttgct aattgcgaat
    20341 gttttcatgg cgaagccgtt agcgtttttg ccgggcccta taccgttact catcataaat
    20401 ctaatttttt aattgccggg atgttcagtt ttttcaatgc aggaagcggt acaaaccaga
    20461 gtaatcatat gtataagcta gggccattac atcagggggt tctcgatcgc ggctcaaaaa
    20521 ctggatcgta cagttattta gtctggccag ctcgtattgg gatattttca gtcgttattg
    20581 ggaaacactt tgcacatctg gatacctccg aactgccttt ttcatacctt atagaaagtg
    20641 aagacaaaag tattctaaaa ccggcgatga atttattcac ttccggcacg cgacgtgata
    20701 ttataaaatg gccggctcgc gatcggcgga aagataccga ccggttggat ttaatcaatt
    20761 ttgatttttt tagcccatat attattgaaa aggctcttcg tgctattgaa atactccatc
    20821 gtcttgatga atcttcacag ccggatcaag aattcgttaa atatcaaggt gcgtatattc
    20881 gaaagtcagt cttaaaaggg gctatccaaa attatgagtt gattccgaaa atcttctatg
    20941 gcaatttgat tatgcagaaa attggttcga tcgataattt aacctgggat aaagtgaaaa
    21001 atatttttac tggtgaagcg gataaatcgg cagaaaattg ggaagatctt agcggtatgt
    21061 ttgctaccag cgcggaaata agtaagatca atttcgcact gcgggagaat cgccttaatt
    21121 caattgttga tttggaatgt gccttaagag atgtccacca aaattatgaa gaaaatacct
    21181 ggcaattctg tctcaatttg ctttttaaac gttttgcgac taacgccttg acttccgaac
    21241 aggtttgcca attgctagat gaatggcagg caagtgtcgt tcgctttaat aatctggttc
    21301 tcaaagatgc tgaaaaggaa tttgctcaat gcagtcgagt cgggtatgga attgatggtg
    21361 atgcgattat ccgtgatcag gactttgaag ccgtgcgagg gacttttgca gaaaataaat
    21421 tcgtggtaga acttaaaaag gaatctgaag aagccgtaaa aaaagtcgag cgtatttgcg
    21481 cggctctccg aaaataaaaa agcctcaaaa acattaaact cactaataac cagtccacga
    21541 attaattcac gggcttatta atttattctt cgatcatata cggataatga taatcttttg
    21601 gcggaacgaa aagttccttc gtcgcgcgtg gcgagaccca acgcaaaagg ttaatcatac
    21661 taccggcttt atcattcgta ccggaagcac gtccgccacc gaatggttgt tgaccgacga
    21721 ctgcaccggt tggtttatca ttgatataga aattaccggc ggcgtggagt agaaatttgt
    21781 ccgcgagcaa gatagcctgc cgatcacggg cgaaaatggc gccggtcagg gcgtacggtg
    21841 aagtcatatt acagagattg agagtttctt cgtatttatt atcgtcatag acaaaaacgg
    21901 tcagaaccgg tccgaatatt tcttcttcaa gagttttgaa atgcggatcg gtcgttaata
    21961 taatagttgg ctcgacgaaa tagccattag tagcgttgcc ttttccgcca gcgataattt
    22021 ccgcatcgcg ggaattttta gcgtaattga tatacgataa aatattatcg aaagcggttt
    22081 tgtcaattac agcattgata aaattgcgga aatcgcgcac gtcgccgatt ttaatcgtgc
    22141 taagagaagc gagcattttt tcttttaatg atggccagag tgattgcggg atgtaagcgc
    22201 gggaaaccgc cgaacatttt tgaccttggt attcgaaagc gccgcgaata agtgcagtat
    22261 ttaactcatc cagatcggca ctgacgtgag caaagatgaa atccttaccg ccggtttccc
    22321 cgacgatgcg tggataacca cggtaattgg caatattttc accaatagtt ttccacatac
    22381 tttgaaaaac ggccgtcgag ccggtgaaat gcacacccgc caggagcgga tcggaaagga
    22441 cattcggtcc tacgttgcgg ccagctccgg ggataaaatt gattacgccc tctggcaatc
    22501 cggcttcttg taataatttc ataaaataat aggcggaaag cactgcggag ctggctggtt
    22561 tccagagaac ggtattgccc attatagctg gagcagtcgg caaattaccg gcgatggaag
    22621 taaaattgaa cggcgttacg gcaaaaacaa aaccttccag gggacgctgt tccaatcggt
    22681 tccattgacc aggtgatgat aacggctgtt gctcataaat ttgttccata aatttgacat
    22741 taaaattgag gaaatcgatt aactcgcatg ccgaatcaat ttcagcctgg taaacatttt
    22801 tgctttgatt gagcattgtc gccgcattga gcgtcatacg ccaaggaccg gatagcaatg
    22861 ccgcggcttt tttgaaaatt gcggcgcgtt cgtgccaggg cataactgac caaaattccc
    22921 aggctttttg agaagcctcg actgccaggc taacttcttt ggcagttgcc tgatgataga
    22981 cagccagaat atgttgatga ttgtgaggca taatgcattt cgcggtttgt ccggtgtgga
    23041 tttccttgcc gccgataatt aacgggattt caatctgttc gccggctatt ttatttaaag
    23101 caatttttaa agcctggcgc tccgaactgc cgggcaaata ggaaaattgc agttcattaa
    23161 cgggttgggg taaagtaaac ttggcgttac tcattgatag ctctcctctc tggcgttgaa
    23221 cttgggatac aatgttaaaa tgcttaacac tgacttaaat taataaacta ttttctgaat
    23281 tggtaggatt aaacgttgtt aagtagtaaa aatttatatt ttagtggaat tttggaaata
    23341 tgaagttgtt tatgacgacg aatacagtat taatcgccat tgtttggctt tataatacat
    23401 atttttcata gtgatttttg cgaaaattat gaggttgaat atttaaagtt gaattaagat
    23461 ttaagctgta ccgattgatt ttcgaaagtg gaaattgtca gcaatttgaa tattacgaaa
    23521 aatgctctaa atagtctata aactgccgat attttttaat aactaatttg gagaactttg
    23581 gcgccgcgaa catgacgctg ttttaaatcc agcagggctt gattggcctt ttcaaacgga
    23641 taaatttcga cttccggttt aatcggaatt tccgcggcta attctaaaaa ttcggctaca
    23701 tcccgccgag tgacattggc aacggattta atttccttct ccagccagag atgttcggga
    23761 taatcaatcg ttgtcagaat ggatttatcg ctattttctt tacggatagc gttgatgacc
    23821 agacgaccgc ctggcatgag atttttcaag gcggcgacga tgggagtcca gaccggcgtc
    23881 gtatcgataa tagcatccag caattctggt gcaggttcat tcgtgtcgcc tgcccagacg
    23941 gcgccgagtt cacgcgcgaa tgcctgttca ttctcactgc gggcgaagac atataccgac
    24001 gtttgcggaa aacgatgacg caccattttc aggactaagt gggccgaggc gccgaatccg
    24061 gttaagccta atttctgtcc attgtataaa ttagtcagac gcaaagaacg atagccaatc
    24121 gcgccggcac agagaagtgg ggcggcttcg gcgtcggtga aaatttcagg aattggataa
    24181 gcgaaatttt cgtcaaccgt catatattcc gcatagccac cattggtgtc ccgtccggta
    24241 gcgcgaaatt gcgggcagag gttttcctga tctgagaggc agtaagagca ttttccgcag
    24301 gcggaaaaaa tccaggcgac gccgacgcga gttccgatag caaagcggcg ggcgtcggga
    24361 ccattttcta ttatttgacc gattacctga tgtcccggta tcatcggaaa tgacgatggc
    24421 ggtgtgcgtc cttcgatctc atccagttca gtatgacaaa tgccgcagac actgacttta
    24481 atcaagatat cattggaagt cggacgtggt tgttcaacat cagcgatagt aagaggcgtc
    24541 tcagtcgttt ttaaatcttt aatttccgag ataaaataag ctttcaattg acaccttcta
    24601 tttcaatacg aatttaacta tatatcaaat tatgtgaaac agagacattt tgaatccaaa
    24661 tagctattga gttaaaaaat aagtttaata gaaaaatgat tgctattttt attcgagtaa
    24721 gcaaatacaa tgtaagtaac caaaaatatt cttttagcct attgacaaaa tagcaaataa
    24781 tcattataat tactataatc actacagaaa gagaatgaca gaaatggtta ttataagtgg
    24841 atattctaaa gagggaggtt taaagtctaa tccaaacagc acctccaatt gacatgcaaa
    24901 aagaacataa atataatatt gaacgtaaga atattttaag aagctttact ttgatatttc
    24961 tattgtcttt tattttccaa atcccgaaat gcgatgaaaa tatcatagat gaaaaggagg
    25021 ctaacaatac ttggactaaa gtggaaagcg gagtgtcagc ttgtctatac gatattgatt
    25081 tcgtaaatga aaaaaatggc tgggtcgttg gagatagcgg tgttattctt aatacaaaaa
    25141 atggaggtga aacttgggag aaacagctat gtctgactga agaaactcta tttgcagttg
    25201 attttataga taccaattgt ggatggattt gcagcaggaa ttctattata aggacaacta
    25261 atggcggtaa aagctgggag ataaaatata gcgaggattt aggtgaaggg cgttttcggg
    25321 atatacaatt tttaaatatg aataccggtt atgttgttgg gggaagggga gcttttggtg
    25381 gtcaaggagt tttaactaaa actgaagatg gtggagaaac gtggcaggat gtaactccgc
    25441 aagatataaa tactcttacc catatttcta ttgttgataa acaaaatata tggatttgtg
    25501 gctttggcgg aacaattctt aataccacag acctcggact gacatggata catcaagacc
    25561 ttaatgtatc ttccgctcaa tatttaacag caattcaatt tgtagatcag tacaatggct
    25621 gggttggagg tgatgataat agttggcttg gattttttcg aacgacagat ggtggcagta
    25681 catggactca aaggtcgcct gattcatggc cagtattttt gggtgttcaa tcattctttt
    25741 ttgttgattc actatatgga tggttggcca ctattcccgg agcagggcct tatgcaatta
    25801 ttcaaactca tgacggtgga cagacgtggg aatttcttcc agaggatatg aatatctatg
    25861 acattcgctc attctatttt atcaataaag aattaggatt tgcggttggt ttggaggaag
    25921 tgaacacaaa tgcagaggga gtaattctta tttatagaag cattgaataa taaacaaaat
    25981 ttatcaggat gttattatga aagcaaaaac agtgaaatct tccttttata ttatcttttc
    26041 gttagaagtt atttgcaaca gctttaatat ttatgcacaa aacaagtagg atggtaatta
    26101 tttagatgag ataaaattgg ctgaacagat tccatcagcg aatctttttt ctatgcctac
    26161 ggaagatctg attcaagagt acttgaatag ccggtatccc cgttatattc tcatttataa
    26221 tgatattcaa aatgctttcg aacatgcata taatgatttt aatggttttc gtgaactgcc
    26281 gattacttta atttagagat actcacacgg atgtttgatt ttatagaaga aatattacct
    26341 aactaatttg gagaactttg gcgccgcgaa catgacactg ttttaaatcc agcagggctt
    26401 gattggcctt ttcaaacgga taaatttcga cttccggttt aatcggaatt tccgcggcta
    26461 attctaaaaa ttcggctaca tcccgccgag tgacattggc aacggattta atttccttct
    26521 ccagccagag atgttcggga taatcaatcg ttgtcagaat ggatttatcg ctattttctt
    26581 tacggatagc gttgatgacc agacgaccgc ctggcatgag atttttcaag gcggcgacga
    26641 tgggagtcca gaccggcgtc gtatcgataa tagcatccag caattctggt gcaggttcat
    26701 tcgtgtcgcc tgcccagacg gcgccgagtt cacgcgcgaa tgcctgttca ttctcactgc
    26761 gggcgaagac atataccgac gtttgcggaa aacgatgacg caccattttc aggactaagt
    26821 gggccgaggc gccgaatccg gttaagccta atttctgtcc attgtataaa ttagtcagac
    26881 gcaaagaacg atagccaatc gcgccggcac agagaagtgg ggcggcttcg gcgtcggtga
    26941 aaatttcagg aattggataa gcgaaatttt cgtcaaccgt catatattcc gcatagccac
    27001 cattggtgtc ccgtccggta gcgcgaaatt gcgggcagag gttttcctga tctgagaggc
    27061 agtaagagca ttttccgcag gcggaaaaaa tccaggcgac gccgacgcga gttccgatag
    27121 caaagcggcg ggcgtcggga ccattttcta ttatttgacc gattacctga tgtcccggta
    27181 tcatcggaaa tgacgatggc ggtgtgcgtc cttcgatctc atccagttca gtatgacaaa
    27241 tgccgcagac actgacttta atcaagatat cattggaagt cggacgtggt tgttcaacat
    27301 cagcgatagt aagaggcgtc tcagtcgttt ttaaatcttt aatttccgag ataaaataag
    27361 ctttcaattg acaccttcta tttcaatacg aatttaacta tatatcaaat tatgtgaaac
    27421 agagacattt tgaatccaaa tagctattga gttaaaaaat aagtttaata gaaaaatgat
    27481 tgctattttt attcgagtaa gcaaatacaa tgtaagtaac caaaaatatt cttttagcct
    27541 attgacaaaa tagcaaataa tcattataat tactataatc actacagaaa gagaatgaca
    27601 gaaatggtta ttataagtgg atattctaaa gagggaggtt taaagtctaa tccaaacagc
    27661 acctccaatt gacatgcaaa aagaacataa atataatatt gaacgtaaga atattttaag
    27721 aagctttact ttgatatttc tattgtcttt tattttccaa atcccgaaat gcgatgaaaa
    27781 tatcatagat gaaaaggagg ctaacaatac ttggactaaa gtggaaagcg gagtgtcagc
    27841 ttgtctatac gatattgatt tcgtaaatga aataagtaga tgagtgagtg gagatactgc
    27901 tacaattctg aatacaaaaa atatgggggg aatatggcta accatagtaa atttttacaa
    27961 gtactgtctt atacacgttg tcaattaatt atattatcag catttatcat ctttattata
    28021 tcttgtgatg ataatacaat aaattcaaat ccatcaaata gtccagacct ttgttatcaa
    28081 aaaatggacg ataattcgca ttggcagata ttcacaaata atatatctgg tactaatccg
    28141 gaaaatatat caaataatcc caataatgac tgttatgatc ctgcatggtc gcccgatggt
    28201 caatacatcg catttcgttg ggataaatca gtaggaggat gcgatatata tttatacgat
    28261 atattcagtg atagtttaat aaatctgaca actgatctgt cagccaccga gagcgcttct
    28321 ctgccagaat ggactcccga tggtgaaaaa atagtgtata cccagaggaa taacggcatt
    28381 aattatcaat atattatgaa caaagatggt tccgataaaa gaaaactgaa tgactggatt
    28441 aattttttct ataatgacag ttatcatata atctgtcaaa gagataattc aattttcaaa
    28501 tctaatataa ttaacagtgt aaacgagttc attattgata taaaagctat aggagaagaa
    28561 tacactaaac tatacgattt taatccaaac acggaggatc ttctatttct aattgcggat
    28621 actccccgaa aaacgaacat tattgccgaa tataatgttc agacacagaa attggacacg
    28681 ctttctgttg cagactcagg ctgggtatat ttacgcccgc aatattccaa tgactatgca
    28741 aaaattgcct ttatagaaag aaattatgat aaagatattt ctaatatggt tattattgag
    28801 aatggtgtta aaaaagacct tgttacttta actgatgaag atgaatggat ttacaatagt
    28861 ccgatagtat tttctcctta tgataattat cttgcttact caaagaatat aaatcaggaa
    28921 gggtatttgg tttggtggaa atcttatcta tacatcgtca atatagagac taaaaaaata
    28981 aagttcatag atggagaatc cgctgttgat cccgtatgga atcctttatt gacttattaa
    29041 ttaaactctc tgcggaaagg aggtgagtta aatattgaat acatcttaaa ataaaagaaa
    29101 aatgataagg tgttttcata agaaaacata acaattcttt tatttgaaaa taccttagaa
    29161 aaaaaatgaa gaaaggagct aatagtcaca tagcatgaca tcgtttacat tcaccccatg
    29221 gggtgaatcg gttttttatg taaagtatca agacatggta tccacctttt agaaataaat
    29281 aggattaatg tagagcagtt ttttgaaatt aaaaggtttg ttgagcgtag cgaaacaagc
    29341 ctttgaacaa cttatgtata tttatcttct tccgaatcat gagataagat gttagacaat
    29401 gctgtcggaa gatccgcaga aagagataca tcgttttaca gggaagataa tgttgatgaa
    29461 gtaattccat cgaattggat taagaaataa tcattgacaa attaatattt acaattcata
    29521 ttacccacag attgaggtta ttaaaatggc gcatagagat taatagtcat ttaataggag
    29581 gaaaaatgaa gaagataatt gttttgttgt ttgtaatgat ttcgattatt caaccacaga
    29641 caattaaaaa agtggttcaa ttcagtgatt cagaatctat cgatatattg actgaatcag
    29701 aaaactatgt cggagcagcg atctcagtta attcttttca agcgttaccg cgtgccattt
    29761 tgttcaataa aaatggtgat atagtatttg aaattgaaga tcctaaaagg tccgggttta
    29821 ttgttgtgga acctgtcgag tctctcggtt attttattac agtcaggaga ggcggtggaa
    29881 aagttcttga tcaaatcaga gcatttgatt taaataccgg gcataaggta tgggaaacta
    29941 atgcaaatgc ttgtgcattt gaattgtcac caaataaaaa gttcatgatt accgtaggag
    30001 atcccggaac cgaaaggacg gggatttttg aaattatcaa tcttaaagat ggcagtaagg
    30061 tagatttcgg gtataattat gatcaatatt gtgcggcatg gttagatgat gaaaggataa
    30121 tttttgtgtt tcaggaatgg gaaaaaggag ttaagcaaaa tgtccaaaaa gactctgtgc
    30181 aatcacaccg atggaaggag atagaattca caaaaaaatc gtttcagctt aaatataagt
    30241 atgatcaagg attaatatca gaaaccgagt acgagaaagg aaaaaaagaa ttagatgaat
    30301 tacaaatgga attggaaaag actaaaccaa aaatgaagag aacgccttca ggcggattta
    30361 ttgaggcgcg tggtccagta acgatgactc caaagaccat taaggtgatt ctttataatt
    30421 taacaaccaa cagcattgac cgggaagaat atctttacaa aggcgatggt agtaaaataa
    30481 gcattggtgt atctccaaat ggatcgaaaa ctataaatgt tgacaaagag aaaaatatat
    30541 acatttacgg ttacgatata atagaaaatg aattgcacag ttatttagtt aaatttacag
    30601 agaatctgaa tttcatctgg actacaactg cagaaattgc taatattata actttttttg
    30661 tcgataataa tgtttatttt agaactaaga tcggaaatga tgtaaaccta atagatacta
    30721 atactggtca tcttcttccg gtagatgaag ccaagaaaaa gaattcgaaa attaacgata
    30781 tcgacttttc ttatcgtcac cggatttata aagtcaaaat actgagaaac acgaatgttg
    30841 attatcaaaa taaaacaata acattttcta aagtggagga ttgaaaaatg agccctgcaa
    30901 gaaattctat tgttttcatt gtatatttat cttcttccga atcatgagat aagatgttag
    30961 acaatgctgg cggaaaatcc gcagaaagag atgcatcgtt ttaccggcaa gatgatgctg
    31021 atgaagtaaa ttcgtcgaat tagtttagga aaaaatcctt gaggaattaa tatttacaat
    31081 ttacattaca tacagattga ggttattaga atggcggtgc attttttagg aaggtttgat
    31141 aaaatttgaa ttatttggat aggtgcgaaa gcattgaaaa ggaaggggga aaatacgaaa
    31201 agtaaattat cagaggtgtt ccttttgttc aaaagtttaa tatctgaact ggatataaaa
    31261 tgtaatttta tcttccgtga caggagtgct aatagttttc gttatagtga agtagtaaaa
    31321 ccactattaa aatatggagt tagataatga ataattgtgg taagtgtact tttattttgt
    31381 tactgttaat attgtgtata tatggcgtgt caaactctca gatagaacaa ccgagtatac
    31441 ctgcaaggga taagccattt ccgctaatta ataaattccc acctgctatt caaaggttag
    31501 attcaataaa ggtaaaatat tacaaagaaa aaaaagaatt ggaattacag ggtaaaaaat
    31561 atgaatattc agataaattt ttgaaagatt acgctagagc tgatattagt agcagattat
    31621 tttattctaa aatagatggt agcggactag aaagagaata taaattgttg acaggtccaa
    31681 tcgggacaga aacgatgaaa ttgttaaaac aactcccata tccgtggggt attttaatag
    31741 aaaaacatgg aatagtgctt gctgaagtat taaatgatac ggttctcccc aaaatgtact
    31801 atcctgtacc cagaccagaa agtgcctcta aagtaaataa ttatgtacta aaagcaaaaa
    31861 taattgatga tatttttggg acaattgaag aggatacagt attaataaga cataatcatt
    31921 taataacaga aaatattctt tttaatcaat ataaagacgt aaaggtgata ttttctctga
    31981 aaccaggcgg tacagtacaa agagaaggat atgttgaacg tatatactta atgggaactc
    32041 caactgagtt tatgtatgtt gctgatggta ttatttatga tgtgaacgat gcgtttaata
    32101 aaaatggcat tacatatcag gaatttaaaa acgaattact tgattttatt aaaagcgagg
    32161 gtattcggcc atgaatatta aaacaaaata tttattattg atagttgaca cctacggaga
    32221 aagattacgg cgcgcaggaa aatatgaata ggtttactgc attattacca gaatggttgg
    32281 gaagactatc aacagttaag caagtatttc aggttttaca aggtttataa caataccaat
    32341 aaacaaatat tagccaccat ctatgacgat gaattccaac tgaatcgcta tggaattact
    32401 attgtataac tacagtatag ctatcgtctc cccattgttt acctttcagt ctccgtctgc
    32461 caaactgacc ggcgactttt ctggcacgca attggtaatt ttcttggtgc ttttttaaaa
    32521 taatttaaaa ataaaaggag accctatgcc caagaaaaaa accgatgaaa accaaactta
    32581 tgagtttaaa gccgaactca aacaactact taacttaatc attcattcgt tatacactca
    32641 gcccgagata tttctgcgcg agttgatctc aaatgcttcg gatgctttga ataaagtccg
    32701 gtttatgcaa ttaaccaatc agaatattct cgatccggaa cgcgagctga aaatcataat
    32761 tgaagttgat cccaagaaac gtactttttc gattgaagac accggcattg gtatgactcg
    32821 cgaggagctg attgaaaata tcggcaccgt tgccaagtcc ggaactctac aatatttgga
    32881 gcaaattcag aaagaaggga agcagatcga cgccgatatt attgggcagt tcggagttgg
    32941 tttttactcc gtatttatgg taactgacga agtcacgatc gaaactcgcc atgccagtcc
    33001 ggattccaaa gcatatctct ggaaatccga tgggaaagga accttcagca tttcggaaat
    33061 cattcgaccg gaacgcggta cccgcatttc atttactcta aaagaagaat acaaagattt
    33121 tgcggaagag tattacgtca aatccattat tcagaaatac tccaatttca tcaacttccc
    33181 gatttatgtt ggcaaggagc gagtaaacag catcgaagcg ctctggcgta agcctaaaga
    33241 tgaaataaaa gaagatgaac tgatcgaatt ttataaattt cttactaacg atattaaccc
    33301 accactggga catctacatt tatcgctgga aggctcggtt aatttcaaag ccttgctgtt
    33361 cgtgccgtcc agtacgcctt atgacatttt ttatgaaatt cgcgaaaaat ccttacacct
    33421 ctacgccaat cgtgttttcg tacaggacaa cgccactgat ttattgccgg aatatttgcg
    33481 gttcattaaa ggtgtagttg ataccgaaga cctgccatta aatatttccc gtcaagtgac
    33541 gcaggacagc ccggtaatgg ccaaaatccg taatgtattg actactcgca ttctagcaat
    33601 gttagaagat tgggcgaaga atgatgtcag caaatatgaa aaattttata agaatttcgg
    33661 tcatattttt aaactaggcg ttaccactga ttttgctaat aaagataaaa tcatcaaact
    33721 tttgcgtttt gagactaccg cgaaaaataa gggcgaattg acttcgttgg acgaatacat
    33781 tgcacgtatg ccaaaagacc aaaaggaaat ttactacctg actggcgaga accgcgagat
    33841 tgccgaacgc aatcccaaac tggagtactt ccggaaacac aatcttgaag ttcttttgct
    33901 ggttgacccg attgacattt tcatcatacc gacgatttac gaatatgaga aaaagaaatt
    33961 tcagtcaatc gaaaaagccg acctgaaatt ggaaaagaaa gctcaaccgg cggaaggtga
    34021 agctcttgaa gagaacctgt taaagtcact gatagcggtt tttaaggaaa cgctgggtga
    34081 taaagtcgag gatgtaatcg tttcgagtcg tttagtggat tcaccggcaa ctctggtcgt
    34141 tggtaaagaa ggactcgagc cccagatcga aaaatttatg aaaatgatga atcaggaaat
    34201 cgagcctcag aagaaaattc tggaaatcaa tccatcgcat cccttgatta aaaatctctc
    34261 ccgtttgaac ctcggcaata gtcatgatcc gcttttgcgt caatcaattc tacagattta
    34321 tgaatcggct ttgttattgg acggtaattt ggaaaatccg actgaatttg tctcgcggat
    34381 ggttgatatt ttaactactg caaccaaata atcataattg taggacaggc attcttgcct
    34441 gtccacaata aattgtaggt cgaaataata tttcgaccat tgcgatttaa caaatcgcct
    34501 tacaattttc ttaatggtta tataacgaca gcctgggaag gctgtcctac gtatatacat
    34561 agttgctaaa aataattgct aatggcgcaa gatttttgtt ggcaacttta tatttcctct
    34621 ttagtttaaa cctgatgttc aggcttatga ggaaagagaa aatcatttgt tcgtagatta
    34681 caccaaagta tctgtgcaat ccggtagcgg tggtaagggc tgtgtagctt ttcgtcgtga
    34741 aaagttcgtt ccaaaaggcg gtcccagtgg cggtgacggt ggacgcggtg gcgatataat
    34801 tgttaaagtt gatcacaatc tcaatactct tcaggatatt aaatatcata aaagttataa
    34861 agcccaaaat ggcgaacccg gtcaaggcgg tttaaaaagc ggcgccgacg gtgcgccggt
    34921 tataattcgc gtgccgaaag ggacattgat acgtgatgaa ttaactaata agattctggc
    34981 ggatctagtc gaagataatc aggaagtagt catcgcccgc ggtggaaaag gcggacgagg
    35041 aaatgcccat ttcgctactc cgacgaaaca aactcctcgt tatgccgagc cgggtttgtc
    35101 gggtgagaaa aaaaaactga ttttcgaact gaaacttttc tcggacgtgg gattgatagg
    35161 tttgccaaat gccggtaagt caactttaat ctcgaaaatt actgccgctc gtcccaaaat
    35221 cgccgattat ccctttacta ctctgactcc caatcttggc attgtcaaat ttggtgaata
    35281 taagagtttt ttgatagccg atattcctgg tttaatcgaa ggcgctcatc agggaaaagg
    35341 gctgggtttt cgtttcctta gacatcttga acgcacgaag gttctggctt ttttgatcga
    35401 agcaacagac ccagagcccg aactaactta tcaaatgctt aataccgaat tggtaaaata
    35461 cttatccact tttgaaaaca aaccacgttt tatcgtaatt actaaaactg atctggtaca
    35521 taaacttccc accctaaaat tcgacaattt gccgatcgtt cccatctctg ccgttaccgg
    35581 tgaaggactg gagttattta tccaacaatc gcttaaatta ctagcgcaaa atggataaac
    35641 tgcaagccat tctcagttta atgagtatta atggattggg ttcacatcgc atattgagcc
    35701 tataccggca ataccctgat attcaagaag tattcagcgc ttcacctaaa cgattgattg
    35761 aggtaccggg cattgatttc aaaatcgcgg aacgaataaa aaataatgtt gatcaccaat
    35821 ttgtcgatca gcaattaaac tggctaaaaa atacccctta taaaatcacg actataattg
    35881 acgaggatta tcctgctcga ttgagaaata tctacgatcc tccggtggtt ttattccgat
    35941 acggtgaatt tttgccgcag gacgaagatg cgattgccat tgtgggaact cgaaatccgg
    36001 atcattacgg caaagaagta gcggctctat tagttagcca attagttgag caaaatataa
    36061 cgatagttag cggatttgcg cgcggaattg acaccatcgc ccacaaaact gctctggaag
    36121 aaggtgggcg aactatcgcg gttctgggta acggcatcga acgagtttat ccgccggaaa
    36181 atcggtcatt gcgtgacaag ttggttaccc aaggcgtcta ttgttcggaa tttccttttg
    36241 gtaccaaacc ggacgcggtc aattttccgc gtcgcaaccg gattatctct ggattaagtt
    36301 taggaactgt cgttatttcc gccggagaaa ggagtggcgc cttattaact gcttattatg
    36361 cacttgacca aaatcgcgag gtttttgctg ttcccgggag aattaccgac gaaaagagtt
    36421 ttggcaccaa tcacctgatt caaaaaggag ccaaattagt cacctcggtc ggcgatatat
    36481 tggatgaaat tgatgctatt cgtcgcttcc cccgtcagcc gcgccagctt gaaattagct
    36541 ttgaatttga aaatgaagac gaaaaagcga tttacgaagc gctttcgacc gatccgattc
    36601 acatagatga tctggctcaa aaactcggta aaaatacata ttcgattttg gcgactttgc
    36661 tctcgcttga attaaagggt gctgtgcggc aattggcggg gaaaatgttc attaaagcag
    36721 gattataaat gcaaatattt aaggaaatta ggggcaattt cgggttgatt ttttcaatct
    36781 gtatttcgat tttgtttctt ccacaaaaaa gtccatctca agatatcttt gaccgaccgg
    36841 ttcgacttta tcaattgtgg gactggattt catataaata tagcaattat ccgacgagct
    36901 taaccgaagg ggtagaactt gtctatttcg gaaccagtgg cggtatttac gcttttcata
    36961 aatacgggcg ctactggact gagccgttta ctaccagcaa tggtttgagc gatgacttta
    37021 taacggcagt cctttatgat gaaacaacgc gttatttatg ggcggcacat aataaaggcg
    37081 tcagctattt aactcccagc agttttcgct ggactaatat ttcaaaagcc gatttaaatt
    37141 taccggaaac gaaccggatt cttcgactcg gtacggatgg taacgcaatc tgggttcaag
    37201 ctgaaggtga ttatctcctc actatcaata agatattggg attttatcaa gcagaggaag
    37261 aatcagcctc agaggcagtt aaatggcaac cgacttccct ggatggtgta ccggatatga
    37321 ggggttatac catagatcag gattaccaaa ttgatgggcg tggtttaatt ttagatcaga
    37381 acctgcgaga atacagggta acggtttttg ccaaaataaa caatgacatt tatggtggaa
    37441 ttgccggatt gggacttttt gaaggcaacg ccaatattaa aaccctccat atccatcctt
    37501 tcggcccgat gcaaaattac attaatgcta tggcggtgga tgataatcgg atttgggttg
    37561 gaggtacccg gagtgatttg aacccaattt tcaccaaatc aggaatttcg tgttataatc
    37621 aaaatgatgg tttctgggag tattttgaga gtctgtttat caatgaattt gcaacgccgg
    37681 tagtcaatga cctagcctat aaaaataatc ggctctggat tggcaccgag cagggtttgt
    37741 caatctacga tcaaaataaa aaccgctgga agcgattatc tatgtccagt ggcttagccg
    37801 atgaaatcat tacttcgatt gcgctcgaag atactctcgc ctggattggt acaccgcgtg
    37861 gactgaatct catcagtatt ccgagttata aagcgcgtcg tcaacgtttg tcgccccgcc
    37921 agaatatctt aaagatcttc aaaattgcca tagattcaaa aaatatttgg attggcaccg
    37981 ataatggaat ttactctatt gacaaattca atcataatgt cgaacattat aatatgtttg
    38041 gcgaacgaat aggtttggat gatgcagtgg ccggtaatat cacggctatt ggatgcggag
    38101 attcggtgac ggtttttggg cgttatcacg acctcctaca attttcatat aatgaaagaa
    38161 aatggactaa tattccactg acagatcagt taaatgaagc cagttttttc gatatcgcaa
    38221 ttgatgggaa ttatatgtgg ctgggaaccg atcggggcgc atttctgatg cgtctatctg
    38281 attattattg cgagaattat actactctcg atggcctggc tggtaacaac gtctttaaaa
    38341 ttttcataga tggcgattgg gtttggtttg ctaccgattg cggtctgaca aaatatagtt
    38401 ggagaaaata tgtcacactc actgagtaaa atatcgttgt tagtttgtgg gttattaacg
    38461 gtggctcttg cccaagagcc ggccgatttc agaggaaacg tggaaccgga aattccgaac
    38521 tttatctatg atgttagtat cacgcctatt gtgaccagag atagtattaa aattgatata
    38581 attattaaag tgccttacag tgcaatccaa tttgtcaaaa aggatacaat attcatcggc
    38641 aaatatgaaa tatcagtgat gctactggat gagaaagaag ttaatgctat cagtaaaatc
    38701 tggactaaaa cccttcaaac caagaagttc gaggaaactc attctaatga actttacgat
    38761 attaataaac taacctataa agtaaagccg agtgattatt cgttaacgat tggagtaatg
    38821 gatttagata ctcgaaagac tactcatcgc aaacgggaat taaaattaaa gaattactat
    38881 aaaaaatcaa ttactctcag cagtatcaac ataatcgaag acattgtcgc tgacagtagt
    38941 ggagatagta ctgatgtttc cgcattaacc gggacactaa gcaataaaag cgaaaaattc
    39001 gacatctcgt ttttcgtatt atctaatgga gggaagggaa cgattaaata tagcatttat
    39061 aatatgaaca aaaaggtagt ccttgaaaac aaaattgatc ataattttaa gaaaggagta
    39121 gaataccaga gaatcagtat tccgcgtcag aaattgtctt acagtaaata tcggttggtt
    39181 cttacggtga aaatcggttc tgaagaagtc acggctgaaa aaataatcca aatccgctgg
    39241 actggtatgt ctgatatgat caataatctc gatgaggcaa tcgaacaact cagatatatt
    39301 gcccgtggtg atgtcattag taagatgaaa aaagccagtg gagaagaaaa aaagaaccta
    39361 ttccttgaat tttggaggga gagagaccca acccccggaa ctgaagaaaa tgaattaatg
    39421 aatgaatatt accgtcgcgt tgcttttgct aaccaaaatt tcagcggtta tttagaaggc
    39481 tggaaaaccg atatgggaat ggtttatatc ttgtttggtc caccaaatga tatcgaacgc
    39541 catccttttg aaattcagac aaaaccgtat gaagtctggt attactacga attaaatcgg
    39601 acgtttattt tcgttgatga gaccggtttt ggagaatacc gtctgattac gcccttggac
    39661 tattacggta gtttttatta atgctaaaat cgcgcaaaat tgccgtcatc gccggagatg
    39721 gaatcggtcc ggaaattatc agacaagtag tcaaaatatt tgaagtgctg aaaaactata
    39781 gccagattaa tctggcaatt acgcatgcgc cttactcggc ggaatattat ctgaatacca
    39841 aagttgcaat tccagatgaa tatattgccg aactcgagaa aaattacgat gcaatcttga
    39901 taggaccatt cggagatcca cgcctccctg acggtcgtta cgcacgcgaa atcatttggg
    39961 gcattcggaa taaacttgat ttattttttg gttatcagcg cgttaaggtt tatgattctt
    40021 ggatgagccc attaaagaat acgcaagaag gattagctga tttttatata attcgcgaaa
    40081 atctggaaag cattaatccc aaaccgggtg gtagttacta tcgcgataca aataaggaaa
    40141 tcgcgatcca atcatctatt tatactcgcc agaatattga tcgatttatt caacaggcat
    40201 ttgaattcgc cgggaagcga aattataaaa aaatcgtgct ggctcataaa aatactttaa
    40261 tgaccaactt gcacgaatta ttgttaaaaa tttttcagga aaaaattacg gctttccccg
    40321 agataaatgc tgatgtgatg aatattgatg ctcttttatt tgaactgctt agcgatcccg
    40381 ccaaatttga aataatagtt gccccgggag acttagctga ttcgatttat actgccggat
    40441 tatttttaca aggcagttat ggaatggctc atatttgtga aattaatcct gtgaaaattg
    40501 gcgcatttcg cattgcgcaa ggatcggcag tcaaaatagc gggacacaat cgcgctaatc
    40561 ctttaggagc attcattgcc acgattgaga tgctgaaatt tatagaactc gatgaagcgg
    40621 ctgaagccgt agagcgagcg cttattcaaa tcctacagaa acacttagtt acgattgata
    40681 tgggaggctt gattggcaca gaagaactgg gaaattattt ttgtgaattt cttaaagaac
    40741 aattatctat ttcccgttaa aattactttg ccgtcttgac tcggctaatg gcttctaaga
    40801 taaaatatcc agctaagcat cctgcggcat agtatataaa atcgctccag ataaagtaat
    40861 ttccgattag agttcgccct acaaaagtat ctcttatatt ttctaaaatg ggtaaatgcc
    40921 atagttgtat gaattcgaga ataccggtag cgaaaaaaac tattattgcg atccgggaag
    40981 atttcaaatt cggaaaaaat aacgagatta ccagacacca gaatatttca taccaaaaat
    41041 cacctgatga gtaactaacc catttttgta aaggtccgga gtagaatttt gtgtaaaaac
    41101 caattgggac aataatcagt ataatcaaga ttttaatagc atacgtgcgt ttaaacaatt
    41161 gtcttaaagt cattgatatt cagcattatt tagtaagttg ggtaaatcat tcgtaattat
    41221 acaagtacaa aattatttgg gtggaaatta aaaagacggg agcaggattc aaaattaatc
    41281 tctgtcaaat tcttaattga ggcgattcat aatttgtctt tttgccaaat tactatcaaa
    41341 ttacgacgca atgtgttcgt taaaaagaag gtccgaaaat gtccgaaaaa tttgaaccag
    41401 tcatcggctt agaagtccat gcccaacttc tgacagatac gaaaatattc tgcagttgta
    41461 agaatgccta tggcgcaccg cctaattcac tgacctgtcc ggtttgtctg ggaatgcccg
    41521 gaactctacc ggttcttaat cggaaagccg ttgaatatgc cattaaaatg ggtctggcga
    41581 cggattgttc gattactgct ttttcgcgtt ttgcccgtaa gaattacttt tatccggatt
    41641 tgccgaaagg ctatcaaatt tcacaatatg acgaaccact gtgccaaaat ggaaagatta
    41701 aaatccaagt aaatggaata gaaaaaaata ttggtataac acgaatacac ctcgaagaag
    41761 atgccggtaa atccattcat ggttttgata atactctggt tgatttcaat cgttgtggtg
    41821 tagcgctaat cgagattgtc tctgaaccgg acttgcgttc accggaagaa gcttacgctt
    41881 atcttattaa gttgaagcaa atccttgaat atttagaaat ttgtgattgt aatatggaag
    41941 aaggcagttt gcgatgtgat gccaatattt ccctgcgacc gatcggagag gaaaaattcg
    42001 gcactaaaac cgaaatgaag aatatgaatt cattccgtgg agtcgaacga gccttaagat
    42061 ttgaaattga gagacaaaaa gctattttag aaaaagggaa aaacatcatt cagcaaacga
    42121 tgctctggga tgaagccaaa ggcgaagccc gccctatgcg ttctaaagaa gagtctcatg
    42181 attatagata ttttccggaa cctgatctag tcccaattga agtagaagaa aattggaaga
    42241 cgaagatatt gaatgattta ccggagttgc ctgatcagaa atattttcgt ttcttaaata
    42301 aatataatct gcgtcattat gatgcggcta tattaacaag tgataaatat cttgccgaat
    42361 attttgaaag ggtaaatgat ttaattaacg atcctcagct ggttagtaag tggatacaag
    42421 gcgaagtgtt acgtacttta tcgcagaaaa atcttacctc gaaacagatt cctctcgcgg
    42481 cggaaaacct tggtgaatta cttaatctga taaaagccgg tacgatttca gccaatattg
    42541 ctaaagaagt cttcgacaaa atggtagaga ataatgaatc ggccaaaatc atcgtcgagc
    42601 gtgaaaaatt ggttcaagtt agtgatgatt ccgaactgga acagatcatt cagacggtaa
    42661 tttccgagaa tcctaacgaa gtcgagcgtt atcgcagagg agctaaaaac ctttttgctt
    42721 tttttatggg acaagtaatg aagaaaacga aaggtaaagc taatccaggc gtcgccaata
    42781 atctgctgag gaaagccctg gataattgat gctttatatc ttaattattg aagtttcgaa
    42841 tatcaaaatt aattgataat aaataacaat tttcataata actctgtttg aagaataaac
    42901 attgttaatc ccgtcagaaa taatctgcgg agagacgggg attcgaaccc cgggtagggt
    42961 ataagcctac acacgctttc caagcgtgct ccttaaacca ctcggacatc tctccaaaaa
    43021 gcgccatagt ttaaattaat tgaaccaata agacaagaaa aaccaaaatt ctgcttatat
    43081 taataggtta ttaattttca cattctaaca tttaaatttt tttatgatta tttggggtat
    43141 taaaaaatga aaaaggatgt ttatctgcaa attattacta ccactgatac gaaagaagaa
    43201 gctgaaaaaa ttgcccatat tctggtagaa aaaggtttag ccgcctgcgt tcaggtcatt
    43261 ggtccggtaa ctagcgttta tcagtggaat ggtaaaatag aaattagcgc ggaatggata
    43321 tgccagataa aaactatcaa agatttgtac aattctgtcg aaaaggaaat actcactcat
    43381 cataaatacg aattgccaga aataattgcc ataccaattt tgacaggatc gaaaaaatat
    43441 ttggactgga tgcggatgca agtcacaaca aacgattgaa ttaacacttt ttctctggtc
    43501 attctgataa taatctgctt cttgggcagt tcattacttt aataattaaa agcgatttga
    43561 agtactaatc gtaactgcta cattagtaat taataatatt aagaacatta gccttgtaat
    43621 atttctaata attaataatg taaagataaa tttaaaattg gcataatata tatgtatttt
    43681 ctattaatta ataaaataat ttgtatgaat ttacttgaat acttaatata gagtcacatt
    43741 ttttgggtta atttataagt ataacaacat taaaaaggaa tcttatatga catcaaaaga
    43801 attttttaaa tttgccaaac aaaatgatgc taaaatggtg gatttgaagt ttactgacct
    43861 gcttggcacg tggcaacatt gtacttttcc gatcaattct tggggtgaaa atacttttgt
    43921 cgaaggcgtt ggatttgacg gttcttcgat tcgtggttgg caggggattc atttgtcaga
    43981 tatgctggct attcctgatc cagatacagc tgtgatggat cgttttttta aagaaccaac
    44041 tgttagtgtt atagctgata tcattgaccc aattactcga gagaattata gccgcgatcc
    44101 acggtttgta gcccggaaag gcgaggcata tttgaaaaaa actggcattg ccgatacctg
    44161 ctatattgga ccggagccgg aattctttat ttttgatgaa gtaatgtatg aacaaaatca
    44221 acataccagt atgtatctga tcaattccat cgagggtgcc tggaataccg gacggtttga
    44281 ggaacctaat ctgggatata aaccaagttt taaaggtggt tatttcccgg tcagtccaac
    44341 cgatacgtat catgatctac ggaccgaaat ggctctcgaa ttgcaaaagg tgggtattgc
    44401 tgtcgaggtg caccatcatg aggtagccac tgctggacaa agcgaaatag atatgcaata
    44461 tatgccctta actaaaatgg ctgaccagtt tatgtggttt aaatatatcg taaagaatat
    44521 tgccaaacgt cataataaaa ccgtaacctt tatgcccaag ccgatttttc aagacaacgg
    44581 tagtggaatg cacacccatt tctcattatg gaaaaatgga aaaccactat ttgcagataa
    44641 tggctatgct ggattaagcc aaatcggttt atattcaatt ggtggtattc tgaaacatgc
    44701 tccagcgatt ttagcctttg ccgccccaac gaccaattcc tatcggcggc tcgttcccgg
    44761 ttttgaagct ccggtcaatc tggtgatgag tgccagaaat cgttcagccg cagtccgtat
    44821 tccaatgtac tccaataatc ccaaagccaa acgtttagaa ttccgttgcc ctgacccaac
    44881 tgctaacggt tatttaacct ggactgctat gcttatggcg gcgattgatg gaattaaaaa
    44941 taaaattgat cccggagaac cccttgatcg tgatatttac gagatgacac cgcaagagct
    45001 ggagaagtat cccaagacgc cgaaaaacct ggcagaggcg attgatgcct tagaaaagga
    45061 ctatgaattt ctaatcgctg atgatgtttt tacgaccgat ttgattcaaa cctggataaa
    45121 ttggaaacgt gagaaagaac tgtatgaatt gtccttaaga ccgcatcctt atgagtttta
    45181 tctttactat gattgttaaa ttaattttct atataataaa gcccacagcg atcgctaaaa
    45241 ggtttgaaga tagcgaataa ttcatcagcc ttcctttttt taatcagata aaatatagaa
    45301 tagcatataa tattttaaaa aaatcataga aaatatttgg atgattttaa tttttgttcg
    45361 taacttactt accggtaagt aaggattgat aaaatgaaat gtgaaaatat tacaagagac
    45421 aaaatcttaa tgaccgcggc cagattgttc gctgataaag gattcgaggc aacctcaatg
    45481 cgcgaaattg ccgaatcatg tgaattaacc aaacctgccc tttattatta cttccaggat
    45541 aaaaatactc tatttgctga aatcatcaat gctattgcca attattcagt tcaattttta
    45601 acagaaatat accaatctga caaggacccg atcaataaat tatatgatat tgttattatg
    45661 caagttacca gattaaagca gaatcctgaa ataaccaaat tcttaattaa tgcacccatc
    45721 cgtaatattc ccaatgaaac caaagttgaa ttttataagc aaatgcaaaa gaataaagaa
    45781 atgcttattc atatcatcac cgaagggaag gaaaaaggtt atctcaaatc cgatttggat
    45841 gtcaatgcct ttttatattg ttatgtagga ggaattaacc agtttattat agatcatatt
    45901 aaaaatggga ctgaaaattt aaatgaacaa aatgctaatg taatcgttag aacacttctc
    45961 gatggtgcac gtaatcacta aactaattta ttaatatata aaggagtatt tgatggaatt
    46021 taaattaaag gttggtgtag tatttttaat tactatcgcc agtctatttg cggaagaact
    46081 taaccttgac attgaaactg cccgggaaat agctttacag cgtaatcctt ctttaaaatt
    46141 agcccgcgag gctgtgttaa aatccagagc cagtgtaaac gaagtcaggg gtagcatgtt
    46201 gccttccatt agtgctttct cccaatttaa gcatgcctgg gaagttggtg gaatgccaac
    46261 accgcaagag ttcaaagatt tgattacgct tcttggtggt acacctcaag aaacaatgtc
    46321 ttttgaaaga gagaatactt ttgtagctgg tttaaattta tctcaaccag tttttaccgg
    46381 tggtttaatt ctgaattcat atcagatgtc gaagattggc gtaagaatca ccgagtcgca
    46441 gctcaaagct accgaacaga aaatattgag tgatgtaact aacgcctatt atggcgtttt
    46501 atttgccaac tcagccagtg aagtagcgga agaggctttg cattctgcgg aacagaattt
    46561 agctcaggtg cagaagtttt ttgatgtcgg caaagcttcg cgttttgatg ttttaagagc
    46621 ggaagttcaa gtagcgaatt ttaaaccgat ggtagtgtcg gccaacaatg ctaagaaatt
    46681 agccgaatca agtttaagac ttatcctggg cataggcgaa gatatcaaca taaactactt
    46741 agaaaaattg tcatataaag aatcggattt gatcaatatg accctggatg aattgatcac
    46801 aatcgccctg gcagaacggt ctgaagtaat tatgctggaa gatagaaaaa aattagccga
    46861 aaaacaggtt aatttagcta aagctaaata ttctccatcg cttgtttttg gtacagcgta
    46921 tcaatatcag gcattcaggg atgattttga ttttactacg gatgatctgg tgaaatcgtt
    46981 caattcatca atcagtttga gtattccgat tttttccggt tttgcgacca aagccaaggt
    47041 tcagcaggcg aaaatagccg ttaaggaagt tgactatcag gctgaatctg ctattgatgg
    47101 aataaaacta gaagtcaagt cagcttattt caaaatgaaa gaggctaatg aaaatgtcca
    47161 aactcaagag aaaaccattg agcaggctaa agaagccttg cgattagcag aattaatgta
    47221 ttccgagggc gcgagcacgc aattggatgt attaaatgcc aacgttgctc tcaatcaggc
    47281 taagatgaac tatcaaaaat cattatacga atacaatgta gctttagcaa atttaaaaaa
    47341 agccatcaat caactttaat cacggagata aaaatgaagt tatcaaaaaa tattctgaat
    47401 ctttttcttg tttgtgggat aatctactat attaattcct gtagtaaaac ccaaaccgac
    47461 gaacaaatga cagagaacat tgttccggtc aacgtcgtta ccgctgaaag aggaactatc
    47521 gaagaaaaaa tcaatttcct aggaaatatt aaaggtttca gggaaatcaa aatctattcc
    47581 ttaatcccta ataagatatt aaaaataaat gtagatgtag gagattgggt taataaaggc
    47641 gccgttttag cagtggttga aagtgataaa atagaacagg cagtgaagca agcgaacgct
    47701 ggtctggaag ccgccagagc gcaactcaga aatatcgaaa cagagtggaa tagaattaaa
    47761 aaactatatg atgataatgc cgtcagtaaa tcgcagtacg acgcaatcac gactcagcga
    47821 gatgccgccc gagcgaacat aaaacaactg gaagccgctg tgtctacagc taaaaaacaa
    47881 ttagaggata cccaaatcaa agccccgatc gccggaattg tttcctcgcg cttattggat
    47941 gttggtgatc aggcggcgcc tcaaatacca gttttcaccg ttgttgaaat ggatgatgta
    48001 aaaataacca tagacgtcat tgaatctcaa ttcaataaag taaaaatcgg acaaagagct
    48061 tacattaatg ttgataccta cccggataca gtttttgtcg gggaaatttc taaaattagc
    48121 cccacgttaa atcttatgac gaaaacctca ccagcggaaa taatcatttc caaccctgaa
    48181 cataaactaa gaccgggaat gttcgccaga gttgatgtca ttaccgatgt tcataaaaat
    48241 gccttaatta tcccgaggta ttctattatt gaaagaactt cgctggaata tatcggtggt
    48301 gacattagtt caactaaaac tataataaac aaatatgttt ttgttgtaga atcgggaatt
    48361 gcaagagaag taaaaattga aacaggcatt gaaaacggaa gaatagtaga agtattacaa
    48421 gggttaaatg acggagattt aattgtttca atgggacagc ataatctttc agattcgaca
    48481 aaagttgaga ttattgagaa agggaaagcg atatgagttt atccaacctt gcaattaaac
    48541 gaggtattac ctttacaatg atctatctaa ttgtgattgg gttcggtatt ttcggactta
    48601 ctcaattaaa attagattta tatccgaata tcaccatacc agtagtcgga gtaatgaccc
    48661 aatataccgg cgtaggccct gaagatatcg agaatgttat cactcgtcca ctggaacaaa
    48721 cgctcgtagc agttgaaaat attgataaaa tcatttcgat gtcgagtgtt ggtaattcac
    48781 tggtaatatt gcaattcgat tgggatgccg atattgatca atcggagatt gatgtccgaa
    48841 aaaatataga tttaattcga gattatctcc ctccagatgc atcgaatccc ataaccatca
    48901 gttttgatcc gtcaatgact cccgttatgc ttatttcagc cagatcgggt caactgggta
    48961 tggcggaatt aagaagagtg atagatgacg aggttaggcc gcgaattgag agactaaaag
    49021 gcgttgcttc agccgcatct ggaggtgggt tagagcgaca aattaaagtc ctgcttgatc
    49081 cgcaaaaagt tgcggcttat ggaataccag cccaacaaat tgttcaaaag ctacaaatgg
    49141 aaaacgtcca gattccggct ggtgttgttg atgatgaata ccaagaatat tctgtccgga
    49201 cttatgggga atatagaact gttgagcaaa tagctaatac agtaatcgga aatgttagtg
    49261 gtacacctat ttatttaaag aatgttgcga atgtcattga cggttataaa gaacaaagcc
    49321 agattgtgcg gaacaatggc gaagacgcag ttatgctctt tatccaaaaa caatcagatg
    49381 ccaatacggt gcagacggca agagcagtag caaaagaatt gccgaaaata atggaattgg
    49441 tcggcaaaga tgtaaaactt gagattgtct ttgattcttc ggaatttatt acagaatcac
    49501 taaacaactt ggtgagtacc ggagtccaag catttatttt ggtttttctg gttctattat
    49561 tctttcttag gtatatcaga agttctttga taaccgcgat ctcaattccg gtttctatca
    49621 tagttacctt ttttgcaatg tatttgctca atatgacctt taacgtcata actatggcgg
    49681 gcttggctct tgctatcggt atgttggtag ataattcgat agtcgttctt gaaaatatat
    49741 ttaggcttaa ggaaaagggg ctagatatta aacaagcatc cgatgaagga gcaaatgaag
    49801 tttcaatggc gatcacggct tcaactttaa caactatagc aattttcgtt ccggttcttt
    49861 ttctaccagg gatttctggt attcttttca aagatctagt tattgttatt gtatcatctt
    49921 tgactatttc tttgctagtt gctttaactt taatcccatt attatctacg caatggttaa
    49981 agggcgagaa aaagaagtca aaatcaaagg tccttagaag aatggattcc gtaattgata
    50041 attttcttca aggacttcat gatagatatg taagaatatt agattatttt ctcagccata
    50101 agaaggtatt tttaattgga ttgatcgtaa ttttcgtgat aactatttta atgtttccat
    50161 ttctgggtgg tgaattttta ccagaggcgg acagatccag tattagaata agtgtcgaaa
    50221 gagaaaccgg ggcatcttta gcctcgacgg acaagacatt tagagagata gagaagataa
    50281 tcagggaaga ggtgccggaa gctgagaata ttcaggttaa cttcggagtt ggcgaaggtg
    50341 ttactgcaat tatggcaagt tctaattctg gtaatattac agttcgctta cttgatgtag
    50401 aagaaagaga gagatcttcc ttcgaaattc aagacatatt aagagaaaga tttcaaaaga
    50461 ttccaggtgc taacattacc attgctagtg gtgaagaagc tacctctatg ttaggtggtg
    50521 gtatcactat caaaatcttc ggttatgata gattagttgc caggaatttg ggtgaccagg
    50581 tagcggaaat gctgaaaggc gtaaaaggag ttgttgacgt tactaaaagt tacaaccttc
    50641 ccaaacctga gtatcagatt ataattgaca gggataaagc ctctgcattt ggtttgagta
    50701 cttatcagat tgccaatata gtcggaacga gcatcagagg aactcgggtt actagatttc
    50761 gtgaaggtgg agatgaatat gatgtaatag ttcaattaga tgagcagttc agaaaatctc
    50821 agggtgatat cgaaaatcta tatattacca caccaaccgg ggctcaattt cccttaaaaa
    50881 atattgccag agttttacct ggtaccggtg aggaatcaat aaaccgagaa gatcaggaaa
    50941 gaagagttag tatatcttgt tcagtttccg gtagagatat ccaaagtgtt acaagagata
    51001 taactgctgg tttaagaaat attaatttcc cacctgaatt tagatgggaa ataggcggcg
    51061 ccgaggaaga aaggcgcgag gcttttttgt atctctatat cgctctagct gcggctgtga
    51121 tactcgtcta tatggtaatg gcttcccaat ttgaatcttt gatagatcca tttatcatca
    51181 tttttactat tccgctggcg atgattggag caatctggtt gttattcata actggaacac
    51241 ccatgagtgt tatggcattg gttggatgca tactattagt aggtatcgtc gtcaataatg
    51301 gaattgtatt agttgactat ataaatcaac taaggtacaa acataattat catctgtgga
    51361 tcgctattct ggcagggagt aagagacgat taagacctgt attaatgacc gcactaacca
    51421 ctattttggg aatgttgccg ctggcattag gcctcggcac cggtgcagaa ttatgggctc
    51481 cgatggctat cgcagtgata ggcggattaa cctttgccac ggtattcacc ttaattttaa
    51541 tacccattat atatcttttc tttgaacaaa ttgctttgaa acgaaagata aagaagggga
    51601 aagttgaagc cgaaccaatt ggaagacccg aaggttttga catcaattta gtaaaataaa
    51661 aaaatccagt aaaaatcggg tagagcaacg agatggtttg attctacccg attttcattt
    51721 taaatttgcg agacaattgg cagggtaata aatgaagtat tgtgatttaa cttgtgaata
    51781 tgcggaattt ccggataaac tgtctgatgg ctcagcaacc tgtcagactt ttattgcaat
    51841 tcattgcaaa cttcttgata gattagttga taaaaatgga atttgtccgg ctgaacaaag
    51901 tgcgaccgac aaatcagtta ataaaggccg ataacgtaga tgttgttatt tctattcact
    51961 aaaagtttac atagctggag tggattttgg cttacggctg tggatattaa ctgatggccg
    52021 aacgacaatt ccccgaaaaa cttctcgaga aaatccgaac tgccccgact gatccaggtt
    52081 gttatattta ccgtaattca gccggtaaaa ttatttatat tggaaaagct aaaaacattc
    52141 gtaatcgagt aagatcttat tttattggag gttataaaga acctaaaaca gccgcattgg
    52201 ttaataatat tgctgatgtc gagtatattc tgactaattc ggagatcgaa gctctgattc
    52261 tcgaaaatac tctaattaag acgcatcacc caaagtataa catctttttt cgggacgata
    52321 aaacttaccc atacgttcgc attaccaatg aaccttttcc gcaagttttt ataactcgta
    52381 gcgttgtgcg agacggctcg aaatacttcg gtccatatac cgatagtcgt ctcctgcgtg
    52441 atactctcga aatcattaaa aacatttttc ctattcgaag ctgcaaacac catttgaatg
    52501 ataaaaccat tgctaacggg cacatcaaag tttgcctcga ttatcatatt aaaaagtgcg
    52561 agggcccttg ccagaggctg gtttcggccg aggaatataa tcgtatgatc aaccaggtga
    52621 tcgcgttttt aaacgggcga accgaagggt ctattgaata ttttgaggaa aaaatgaaaa
    52681 ctgccgcggc gagtctgcaa tttgaaacag ccgcggctta tcgtgataag atttccattc
    52741 ttatgaatta ttctaatcgt caatcagtcg agttgaatga tgaactcgat cgcgacttaa
    52801 tcgccgttct gaaagaagcc gaaaattgtt gtgctgtcat atttcgggtc aggcagggaa
    52861 agttgatcgg tcgcgataaa tttttactgg aaggaaccga aaactatgat tacccggcaa
    52921 ttactcacga tttgctcctg cgctattatg ataatgcaac cagtatccca tcggaagtat
    52981 tgctaaacta tctaccggaa gaacaagaaa tgcttgaaaa atggcttatg tctcaaaaag
    53041 gtgcccgagt cagtatcctc cagccgcaac gaggcgataa agctcgattg atgaaaatgg
    53101 ctatccgcaa cgctgaattg caaatcagtg agttgcaaat gaaacagagc cagaaaaccg
    53161 aattcgtgcc gaaatcgctg gttgctctcc agaatgaact tcgactaaaa gaattaccga
    53221 gacggattga ggcttttgat atatctaata ttggcggtaa atttgccgtc ggttctatga
    53281 ttacatttat caacggcgtc gcgaaaagca gtgagtaccg gcgattcaaa ataaaaactg
    53341 taaccggaag taatgattat gctatgttag cggaagttgt tcgccgccga tacacacgcc
    53401 ttcttcgtga ggaaaagcct ctaccggatc tgattctaat agatggtgga aaaggtcaac
    53461 tgaatgccgc tgttactgta ttacaggaat tgaatttatc cgcaattcct gtaattggtc
    53521 tggcaaaaag actggaagag atatttattc cggggaatga tgaaccaata ttattagcgc
    53581 gcaacagcct ggcactcagc cttttacagc gagtaaggaa tgaggcacat cgctttgcca
    53641 tcacctatca tcgaaaaata cgtggaaaag gcggaatttc taccaaactt gatgaaatcc
    53701 cgagcttgag ccagaatacc aaaaaggctt tactgcaaca ttttaaaacg atcgccaata
    53761 taaaatccgc cagtacggaa gacttgatga atgttaaggg gattgggcta aaaatcgctc
    53821 aaaaaataaa acaatatcta taaaatagga atttaaatga agattaatgg attgaatgtc
    53881 agcgatttag ctgatgaatt tgggactccg ctttatgttt atgattttga tcgtattaaa
    53941 gacaatgctc aacgtttgcg gagagctttt gattctgatg aaattgaaac tgcctttttt
    54001 tatgctgtga aagcgaattc gcatcccgca attgtacaac tgttgaaaga ttgtggttat
    54061 ggctgtgact gcgttagccc aggtgaaatc gaaattgctt tgaaaactgg agtaaaacca
    54121 tcggacataa tctttgccgg taattacgaa agcatggaag atttcaatgt tgccgtcgaa
    54181 gcgggagtaa atatcatttt ggatgatatt acctcgctgg atcgtttgtt acgaattcat
    54241 tgtcctgagg taataagttt tcggattaat ccagggaagg ggagaggtaa gtacgaacaa
    54301 attaccaccg gtggtgagaa ggctaaattc ggtgtgcccc acgaaaaagc gtggctcgct
    54361 tatgaaaaag cccgtcaggt agggattgaa cggtatgggg ctcaaatgat gaccggttcc
    54421 ggcattctcg atgaagaaca ctttccgcga atgctggaat tattactgaa catcttaggc
    54481 caaatccacc aaaaactcgg gataactttt gaatacgttg atatgggtgg cggattcgga
    54541 ataccttatt atggagacga tcatgaactt gatattcaat atgttggaag agaatgcctc
    54601 aaggtgctta aagccaagtc tgaaagttta tcaattggca aaccggcttt acatttagaa
    54661 cccggtcgtt acctcgtcgg cgacgccggt tacttggtga cacgagttct aggaattaag
    54721 gaaagctatc agacatttgc cggattggac gccggattca atactttgat tcgttcagca
    54781 ctttataaag cccagcatcc ggttattgtc gatggcaaag aagatgaaaa agaactctat
    54841 ccgattaatc tttgtggtca gatttgtgaa aatacagata ttttcaccgt agggcggcct
    54901 ttacctaagt tagaagaagg cgatttgcta atttttactc aagctggtgc ttacggaagt
    54961 actatgtcaa tgacctataa tcaccgactt cgtccggctg aagtagccat aattaatggc
    55021 aaggcgattg aaattactcg acgcgagacg atgagtgatt attggaatcg aattacatat
    55081 ccgatacttc tttgacaatt aacacattac tggagtaatc aatggctagc cagattgaac
    55141 cggaagttaa cctgcaaact gctattcaac acggtttaac tgaggaagag tggaataagg
    55201 tattaaagat tttagggcgg acgcctacct ataccgaact gggtattttt tcagtgatgt
    55261 ggagtgaaca ttgtagctat aaaaattcga tagctttgct taaaactctg cccagaaatg
    55321 gcaagcgtct tctggttggc gctggcgaag aaaatgcggg gttagttgat gttggtgatg
    55381 ggctggcgat ttgttttaaa attgagagtc acaatcatcc gtcagcagtt gagccatttc
    55441 agggagcggc gactggggta ggagggattt tacgggatat ttttaccatg ggcgctcgac
    55501 cgatcgcttc actcgattcg ttacgttttg ggccattgga ggcaccacgc aaccgctatc
    55561 tcttcgataa cgtggtaaaa ggaatcgccc attatggcaa ttgcgtcggt gtgccgactg
    55621 tagctggcga aatttatttc gataattgct atacagaaaa tccattggtt aatgttatga
    55681 ctattggctt ggtcaaacat aaaaatgtca ttagcgctgt tgccaaagga gaaggaaata
    55741 ccataatgat agtcggctct tctacaggaa gggacggcat ccacggagca acttttgcat
    55801 ccgttgaatt aactgaagaa tctgaaaatc ggcgttcttc agttcaggtt ggagatccct
    55861 ttaccgaaaa actattgctg gaggctactc ttgaacttgc caagcaggat tttttaatcg
    55921 gcattcaaga tttaggtgcg gctggaattg cttgctcgac ttccgaaatg gcagtgcgcg
    55981 gcaaatccgg aatgattatt aaccttgatt ctgttcctct ccgtgaaccg ggtatgagcc
    56041 cctatgaaat tatgttgtct gaatcgcaag aacggatgtt agttgtcatt cgaaaaggtt
    56101 ttgaagaaga gactaaggcg atttttgaaa aatgggattt aaactgtgtc aaaatcggta
    56161 aggttattaa ttctggtaat ttggatattt ttatgaatga taaaaaggtc gcttctctgc
    56221 ctgcatggca tctggtgttg ggtggcgggg caccggttta tgagcgagaa accaaagaac
    56281 cggcttattt gaaaaaagtg caggggcgta atttgaaaaa aatacccgag cctaaagact
    56341 acaacaaggt gcttctgcgg atattatcgt cgccgaatat cgcggataaa tcctgggttt
    56401 atcggcagta tgattattcc gtgagatcaa atacagttgt caagcccggt ggttcagccg
    56461 cagttattcg tattaaaggt actcgtaaag cactggctat caaaaccgat gggaatggtc
    56521 gttatactta tctcaacccg tataaaggtg gtgcgatcgc cgtcgccgaa tcggctcgaa
    56581 atgttgtctg ttccggtgcg cgaccgattg cgattaccaa ttgtctaaat tttggtaatc
    56641 catacgaccc agaaatttat tatcaattta aaatggcggt gcagggcatc ggtgatgctt
    56701 gtcgtgtttt cgatacgccg gtaactggcg gcaacgtcag tttctataac gaatctccta
    56761 aaggagccat tttcccgacg ccagtaattg gtatggttgg tctgattgaa gatcttaaaa
    56821 acgtgactac cccctggttt aaaaacgacg gcgactttat cattttatta ggagccttaa
    56881 aaggcgaact ggggggaagt gaatatctaa aagtggttca taacattatt gctggtgatg
    56941 ctcctgatat agatttaacg tttgaaaaaa gaatctttaa tgctaccttg gaagcaattc
    57001 aaaaggggat tgtgaattca gcaatagata tatcggacgg cggtttggct gtggctctgg
    57061 cagaagcttg catctctaat cctgaaaaac cacttggcgc ctcagtctat atttcgcgca
    57121 aacttagaaa tgatgaactg ttttttggtg aaagtcaatc agttatcctt atatccatta
    57181 atgaaaaata tttacttgat atagaacgaa ttgcctctaa gaatatcatc ccaagtgtga
    57241 caatcggacg cgttaaagac aacggtcgct tgacaattaa taatattatt gatttacctt
    57301 taactgaact gcagaaagcc tataccagaa ccattccagg ttttatggat atttaataga
    57361 atccacaaat attttattaa aattggaaaa ttagtctgaa tagccgttcg gatttttctt
    57421 ctgccagttc caggtgtctc gacacatgtc atctaagttt cgttctgctt tccagtcaag
    57481 ttcgcgattg gctttgcttg gatcggcata acattcggca atgtctcccg ggcgtctgcc
    57541 aacgatttta taaggaattt ttcgttgaga ccatttctca aaagtcgtaa ttacatcgag
    57601 tacactatat ccctggccag tacctaaatt gtaaacacaa taaccgggat ttgtcagtaa
    57661 tttgtctaat gcttttaaat gtccttgggc taaatcaaca acgtgaatat aatctcggat
    57721 accggtgcca tctttggtgg ggtagtcatt tccataaacc tttaaatagg ggagacgtcc
    57781 taccgcgact tgagaaatat aaggcattaa attattagga atgccattag gatcttcgcc
    57841 gattaaaccg gattcatgag ctccaactgg attgaaatac cgtaatataa taacattcca
    57901 atccggatta gcgatttgaa tgtcttttag aatcgtttca ataatatatt tggtattacc
    57961 ataaggattc gcaggtttga ttgggaaatc ttcggtaatt ggaactgatg tcggattgcc
    58021 gtacacggta gcggaagaac tgaacacgat gttcttgaca tcgtatttaa tcatcatctg
    58081 gcagaggtta gtcgttcccg taatgttatt ataatagtaa agcagaggct tttggacgga
    58141 ttcaccaacg gctttcaaac cggcaaaatg gattactgct tcgaattgat tagaagaaaa
    58201 tactttttca agttcaatga tattacatag gtcaatctta ttgaatatca attctttacc
    58261 agttatagtc ttaactcttt ttatagactc tattttgcta ttggaaagat tatcaataac
    58321 cgttatctca tggttttgat tcaacaattc gaccaatgta tggcttccga tatacccagc
    58381 accgcctgtt aataatattt tcatagtatg tccattttag ttgctaattt ctccaattat
    58441 tccaatagga atgggtggaa atgagattcc aaactgaaaa ttatccatct cgccaattcc
    58501 tataaaaaaa cctggtttat aatttttaaa tccaattatg cccgggaata tttccagacg
    58561 aagattataa aacttaggac caaataaagt taaagatccg agtaatgaat tatttctatc
    58621 atagaaaaaa gctacagcgt aatcaaggtt tggaataata aaacgactat catattcttc
    58681 attaggcgtt aatttagcta caacagcacc ggcagagaat gatatcgcat cagtttcatt
    58741 tatggtatgg ctaattccaa aagtagagac aacaccccaa tagccaaaaa gcgcattttt
    58801 atcagtcgtt tttaacttaa tctttgtggc atattgctgg ccggcattct ctaaaaaata
    58861 gttatttgga ttaaaatagg gatttagcga ccaatcgtaa attggtattt tatttgagaa
    58921 aaactcttgt cctgcttttg tactgaaaag taatattcct aatggattga atattaacat
    58981 atctgaaatg ggatctacgt tagtgcctga atatgaacca ttttctacga tttcatttac
    59041 caattgatat gatattgtag ttattattga taacagatta gggtttggat attcatggta
    59101 atcataccat tcagctagtt tgcgatattg cataccattg ccaatcaaat gtaaaccaat
    59161 attgggtaaa aattgggatt catttttatt tatttcaagg ttaaatattt cacgattcag
    59221 aaaattttta acaccataat ttctaatatt atctatagga tgcaaaacat tatcaataac
    59281 attattaaaa ccggttttag atggctggtc aaaaatattt ttattgtgag caccatttcg
    59341 caaaatatca aacgaaccgt tgatgaacaa actaattgga ttaaaaattg attcagagcc
    59401 ataatcaatg tcaggattgt agaaataata ttcataatca ttgccgctgg cattgattac
    59461 aacaattaac attaacaaaa taattttttt atacatatca tataactgag aatttaatca
    59521 acagttgcat aattcaaaat gaattgaatg atacttacaa atgtctaaaa atgccctaaa
    59581 atcaatatac gcataagata tattatgtat agttgtagat aaatttaaag aagttatata
    59641 taggataaaa ttgatttatc ccttcctacc ttctgttata ctattaatga attatattct
    59701 tggactgtgt ttttataaaa tgctattatt agtaataaca attctctgaa tatcaattaa
    59761 ttattgaaat atatttcaat ctttaataac gaaatacttg aagaacaatt ctttagataa
    59821 ttttgcacct ttttcactta atgatacagt tgctgattta tctttagggt cactaattaa
    59881 acctttttga tataaacgat ccaaagcctc aaagtcaaat cctttccatg ccttagtacc
    59941 atattgatca gatgaactag taagaaataa aagagctaaa atcatattat cgactttgtc
    60001 tttgtcgtat ttcatttata caatcctaat tatttggtgc atttaatgta tttttataca
    60061 ttctctctgg aaaattcttt ggttgtaaat caaaaaagac tttgtaacct ttagaattct
    60121 ctatgatttg aaaagcatca taaagttctt cagtaacggt ataggcttta taatcgagaa
    60181 tattatcatt aatggttata atttgataga gttgagtatt ttctgccgca cgatccatcc
    60241 agcggatttt attaatatta tataattttg ggccgctgac tgaaacaaca taaaccggtc
    60301 ctgttggatc attaggacga atagtcaaac gctctggatt atttccgcgt gcataagtgt
    60361 ggtcatgacc gttaagaatt aaatctacct tatattttat gaaaattgga ttccaggtat
    60421 cacgaagttc ctgataatca cgaccttcag ctccgcaata aatgggatga tggaaggcta
    60481 caaccgtcca tttattaggg ttatttttca aaatttcttc caaccattgc gcctgtttct
    60541 ctaactcaaa attggaatta agtacaatca tacgcaagcc ctgaaaatcg aaatagtaat
    60601 tagtctcagg aagttcctcg attccgttta aaggtaaagt gaattgcggc cgccagtacg
    60661 gcataaaaaa ttgctcgccc caatttccat cataatattc gtggttacca attgctggca
    60721 taatcggaac cgtggcattg atccagctgg cggctgtgaa ccattcattt aaagccggat
    60781 agtcacgagc attattgact agatcaccgc catgaatgat aaaccgcgca tccggcgcat
    60841 ttttatatgc agtgcggatc gctcgtgacc atagagaaag tacatcttct tgcccatcgc
    60901 ctaagtaaat aaatcgaaat ggttctggtt ttttactcgc agtgcggaat tgaaaccact
    60961 cactccatcg cttgtctgaa ccaacccggt aaatatataa tgtatttgat tgcaaaccgg
    61021 taaaagttac cgaatgataa ttggcgcttt ttccatcgaa attaaggagc tcggtcttgg
    61081 caggtattcg cttggcttga taaacaaagt tgggcgatgc atcggctagc gctatttcac
    61141 cataagcaga tttaatgctg gtgtctgtgc gccaggttac cgattgagtg ctggtgggat
    61201 catcactcca ggaaagaatt agatggtcgg gatattgagt aggatcggga taataagccg
    61261 cccgtgatgt gtcccgagca actaaaattg gagagaattg cccgattatc aataaaatca
    61321 atatcaattt taacagatta ctgcaagtca aattttttct ggtgattttc tgcatgataa
    61381 aattgctcct tttgtgcctc taccctttca tttaattata ttacataacc ttaaatatca
    61441 tctaaataaa tcaaaaacat taacctatag aaatcaacgc tttcatcaca ccgtcccgat
    61501 aattagcaac cagttcgaat gcttcggcgg ttttttcaag tggaaaatga tgcgtcgcca
    61561 ttgcattcat atttaccaga tggctttcta ataaatcgat agctttttga gtgcaacgga
    61621 cttggcgacg gacattaatt atggtgatct ccctacgtcg aagctcatga attggaaatg
    61681 aaatttcatc catttcaggg atgccaacca atactagaat tccacctggt tttagcaatt
    61741 gaactgcctg actaattgct gttgtatctc cgctacattc ataaacgacg tccagttgta
    61801 gaggctctcg tttattaatt tcgctaacta cattggttaa atctggatta ccgcgccacc
    61861 caggatttaa ttgttgggca aaatcgaggc gttcctgaat tttatcagtt acatagatat
    61921 taccgacatt ttttattcgc aagatacaaa aaactgacat accaatcggt ccgactccca
    61981 gaatagcaac attagcatta tccggaagca gggatcttgc gacggaataa atcgcaatcg
    62041 ccagtggttc ggataaaaca gcctgctcga aggtcatttt atcactgatt ggatagcaac
    62101 aattttccgg gagcacgata tactcgcata aagcaccgtt tagttgtttt ggagtaccca
    62161 ggaatttcag gttgcggcag gtattttcac gacctgagcg acactggtcg caattagcac
    62221 aataaactgt cggatcaatg gcaatccgct gaccgatttt gactcgagta actttattcc
    62281 caaccctttc aactactcca gcggcttcgt gcccgatgat gaatggaaat tgaataacct
    62341 gcgatccaat tttgccattt ttataataat ggatgtctga gccgcaaact ccgaccattt
    62401 taactcgaat taatacatcg gtgtcattta caattgttgg atcggcaacc tgctggatgg
    62461 tgagttgtct gattccggtg agtgatactg ctttcattat aattaaattt attttaaaaa
    62521 taaaatttat ctatgattat ccaaccagat ttaaacgatc taaaaatgat cacaataact
    62581 cgttccgcag ttgttcgcca ctcttgggtg acaataaagc cggcggaatg tcaaacgcgg
    62641 ttctcgcacc tattgcgccg ctctgtgcta aacgataagc cgcacgggca tagcaaacca
    62701 atacatttcc ggtaaattct ggattagaat ctaacttcaa tgaatattca ataatgtgcc
    62761 tattccctgc tccggtatgt ccgctccgaa ttacaaatcc accgtgattc attttgctat
    62821 gattcttctt aaattcatct tcggaaataa aatggacaac cgtttcattc tcaatgaaat
    62881 agttgggcat attttttata gtattttcga cataatctct atccgcatct tcttcaagca
    62941 cgacaaaaca ttctcggcga tgcatttgac gagtggtcaa ctgcggattg gcaccactac
    63001 gaactgccac aagtgattcc tcgattggta tggtgtattg gatagcattt ttgacacctt
    63061 taatccgccg aatcgcatct gaatgtccct ggctaactcc ttttccccaa aatgtgtaac
    63121 ttttgccatt aggcaagatt gcctcggcat acaatctatt gattgaaaat aacccggggt
    63181 cccaacctac cgaaataata gccaaatggc cattatcgcg cgcggctttg tcaactttag
    63241 cgaaatattc cggaatcttg gcgtgcgtat cgaagctatc tactgtgtta aacaacgaag
    63301 cgaaagccgg tccttgcttc ggcaaatctg tggctgaacc accacataat atcatcacat
    63361 caattgctga ttgcatactt gctaattcat tggcgctcag caccatagta gatttacttg
    63421 ctgttgtaaa tttagctggg tctcttctgg tgaaaatggc tactaattca atatccggat
    63481 tctgagttac agcctgttcc actcctcgac caagatttcc atagccgacg ataccgattc
    63541 taatttgttt caaagggata ttcctttcat ttttatttgt tgagcttttt ccagccgttt
    63601 ggaattccat aattatatat gatgtcaata gcttttgagc gcgatatttc accaggattg
    63661 atacctaact ttccttctgg gatagctaca ccgagttcgg cgaaatactc ttgccatatc
    63721 ggaaaagttt ctcctgtact tggatcaaca aatgtcattg gttgcagttt aaaaagtgac
    63781 tgattatcag tcgatttgag aaaagcctta taaattactt cagagcaata ataggcatcg
    63841 ttttcaatgt cgaaaaaatt gtcgtaaggt ttaccaataa gtttttgggc ttcctttaat
    63901 gccggtagaa tttggtggcg atatttcggc ttgagtcggc tgactaagac tttgggatga
    63961 ttttgattgt cataacttct agctaaaaat tcaaagagcg gggtcaaact gactccggca
    64021 agtatcgctt ccagcacaaa aattttaccg ctcgaatctt tggtgacgat gcctatatgg
    64081 ctgagactcg caccttgata gcctttcgta acggtttcaa tcgcgtcata aaacggactg
    64141 ccgtctaaat cttgaaacat taggtcacca acttctgggc taaactcact agttggttcc
    64201 gattgttttt tagaacatat agtagcaaac aggcaaaatg tcacgatcaa tacaagtaat
    64261 tctattctat atctcatttt cacgccgtta ttttaagaaa caatgcggcg aatttcaagc
    64321 aaattacaat ccctaatttc tcggaatatt aaatctccgc aagctgattt gaaaaatgga
    64381 tgattgatag attaatatta cctacgaaac ggcgaaggta ttttagagca acaaagtcag
    64441 gagagttatt ctctttagga aagataatgc caattatttt gcagggtctt tatctatcct
    64501 gcgtctaaac gagaaccatc aaattgttat cagcaaatca gcagatatgc atctgaaata
    64561 tcggaaaaga cctgaagtct aatcacttct gcaagaccag ttttttcgtt tctgagtagg
    64621 cacctgcctg aattcggtag aagtaaattc cactcggtaa tcccttaggc atccattgga
    64681 cctcgtgaat tccagcggtt tgaaactcat tgatcagcac atctatctct ttcccggtca
    64741 tatcgaaaat tttcagagtt acaagttgat cttcaggaat tgaatattta attacagtga
    64801 cgggattaaa aggattagga taattctgtt ctagtttaaa ttcataggca accgaggaat
    64861 ttgctaattt taggcctgtt ccagt
//
LOCUS       DAOSMP010000009        64348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_487_length_64348_cov_16.004200, whole genome shotgun sequence.
ACCESSION   DAOSMP010000009 DAOSMP010000000
VERSION     DAOSMP010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 64348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64348
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_487_length_64348_cov_16.004200"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PKX09_02610"
     CDS             <1..345
                     /locus_tag="PKX09_02610"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOU16747.1"
                     /translation="NSKIKKAITDPQKIRRGDPGRPEICLVYTYHEKFNPAQCPEIYA
                     GCTSGALGCVECKMKCGKAIQSVIAPYRTRRMYYAAHQSEVIDILIDGEKRARQVAQN
                     TMADVHNKMRIG"
     gene            399..1139
                     /locus_tag="PKX09_02615"
     CDS             399..1139
                     /locus_tag="PKX09_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOU16748.1"
                     /translation="MSYNVKLNVYEGPLDLLLFFIKRDEINIYDIPIAYITDEYLNYL
                     QLMQSLNLQLAGEFILMASILIRIKVQMLLPRPETEKLEDIEDPRTELVQMLVEYQKY
                     KEIGEQLKRLEANQSRHFPVTLTSDQRNYDPELFLGDITLIDLGLAFQQILEKLPKVE
                     PVNLEIMKVNIRQQILFIRECLKKRSRIRFSEIAAKLTDRLEVVVTFLAILEMIKSME
                     ISVSQKDIFGEIWITKKKTIQDNVISNA"
     gene            1120..1680
                     /gene="scpB"
                     /locus_tag="PKX09_02620"
     CDS             1120..1680
                     /gene="scpB"
                     /locus_tag="PKX09_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOU16749.1"
                     /translation="MLSQTHRIVLALLFGATEPLTKSQVDICLEEDIDLTQIIADLNQ
                     YLETEGLPVWIQLVAGGYRLMTRAEYEPWLSRLFQNKGKLRLSRSALETLAIIAYKQP
                     VTKSEIDAIRGVDTSLRPLVEKNLIKITGRQEGPGRPLLYGTTNHFLEYFGLASLQDL
                     PQVKELAEIAGEDNLQSDIVEQDATE"
     gene            1667..2380
                     /locus_tag="PKX09_02625"
     CDS             1667..2380
                     /locus_tag="PKX09_02625"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOU16750.1"
                     /translation="MRLNRYLAQGGFSSRRKCEDLIRGGRVKINGCIVTNLATQVKET
                     DQVTVDNRRIVPPKPVYILLNKPAGYITTMHEQFGRKAVTDLVNNAFAKPIGRLDMAT
                     TGVLLLTNDGELAYRLTHPKFGVERIYRAAIAGRVSPDIKKQVAEGISIGGAQKASGQ
                     ILTIKYLDGFSSVTIELKEGQNREVRRIFAVLGYKVISLDRISFAGVRYSGLERGQWR
                     NLNESEVRRLKTLVGLADK"
     gene            2504..3223
                     /gene="cmk"
                     /locus_tag="PKX09_02630"
     CDS             2504..3223
                     /gene="cmk"
                     /locus_tag="PKX09_02630"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOU16751.1"
                     /translation="MGIIIAIDGPAGSGKSTTAREVARRLNFTYIDTGAMYRAITLAV
                     IRQNVAVEDHQSVIKIAEQADIQFEWADGNLRTILNGEDVSNEIRSAQVARLVSPVSA
                     IGGVRDILVKRQREMAKKANIVMEGRDIGTNVFPDADFKFYLEADLETRARRRINDYQ
                     KIGQNLSITEIMTEIEQRDKIDSSREHSPLKKAPDAIVIDTSNLKFEEQVEMIIQIVK
                     SSELFKKIEKAPVINGTNCKN"
     gene            3248..5461
                     /gene="rpsA"
                     /locus_tag="PKX09_02635"
     CDS             3248..5461
                     /gene="rpsA"
                     /locus_tag="PKX09_02635"
                     /inference="COORDINATES: protein motif:HMM:NF004953.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOU16752.1"
                     /translation="MLEEEQTLNPKPSEESEVIPDINTADPFAEPVTARDATIEERTS
                     ATSDYTTELVTSGPLSEIVRVYDLKDIDKLNEVQAPKAEDHTKIYENSLKDIAKDQRI
                     LGTVVAINENEILVDVGFKSEGIITREEFEEGELPKIGDQIEVFVDVLEDENGQMILS
                     KRKADFMRIWERIKEIYAAGETVEGQIVSRIKGGMVVDIMGIDAFLPGSQIDIRPVTD
                     FDSYIGKTFEFRIVKLNELRKNIVLSRKELLEESMKEKRDDLLSKIKVGDILTGRVKN
                     ITDFGVFIDLGGLDGLLHITDISWGRINHPREVVQMDQELTVKVIDYDQEKQRVSLGL
                     KQLTTHPWEGIESKFPVGTIVKGKVVNITNYGVFVELEKGVEGLIHISEISWTQHIKH
                     PSEVFKLGDEIEAKVLGIDSAERKISLGYKQLEPDPWESIEQKFTVGSVHKGIVRNLT
                     PYGAFIELQEGVDGFAHISDMSWTKKIRHPRELLKKDDEVEFKILEISKENRKISLGL
                     KQVTEDPWPMIETLFSVGSIVEGEVAKVTDKFIVVNLEHDLEGIIPLGQMPKKDRKDL
                     SKAVEIGEKLQLKVLEVNRQEKKIILSREQAIPREPKTEVEAYMMQQAETTNKLEIPS
                     EIIQKIAESEEKTQEKEEKSAAKAKSPAKKAKKAENVEKPEDLTTEEEKPKKKSTKKV
                     ISEEIPAEEKPEVETKKETEDSPEKTRKNSAKSTSKKSKPQAESEKPAADDTAEK"
     gene            5535..6572
                     /locus_tag="PKX09_02640"
     CDS             5535..6572
                     /locus_tag="PKX09_02640"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOU16753.1"
                     /translation="MNPSLKEKRRKFNEKLSYRNVRQAVRKWITTNTRTKIWIFVFTV
                     FLWLYVVLNNTYSYTFSAKLEVRNIDPTKTTIEKLPSQVQASFSGKGIDLFYLILSPQ
                     QSIKFVVDCYSIKQYYDFPLNEYFKNNPEKVITPRNINVKLDHIIWPETLRVVLDDLV
                     TAKVPVKPEVEIQLAPGYVLAGSMRIIPDSVTLRGPRTYVRQYNEVPTKKLVLTNVSS
                     SISQEIDLEFVPKYNVYIDVTRVRILQKVDQLGEKKLTDIPIRVINLAQDQKAEIIPP
                     TATLTISGGIEKLKTVQPEDIKIVFDFATDWRPDESFYTPTVELPPGLLNWWNLEPDT
                     FEIRIIRERQP"
     gene            6573..7577
                     /gene="tsaD"
                     /locus_tag="PKX09_02645"
     CDS             6573..7577
                     /gene="tsaD"
                     /locus_tag="PKX09_02645"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOU16754.1"
                     /translation="MLILGIETSCDETAVAILNDNEILVNLVSSQLLHQQFGGVVPEF
                     ASREHMRLLDQMVKEAFVQTGLQPENLDGIAVTRGPGLMGALLVGLSFAKGLAYSLRK
                     PLLGVNHIEGHIMANFLTCPDLKFPFLCLLVSGGHTQIIQVNDFGNYQILGRSVDDAA
                     GEAFDKAARILELPYPGGPQIDQMAHRGDPNFYQFPRAKVRSEQYDFSFSGLKTAMLY
                     LVRQRGQKWAQENLADLCAAYQEAIVDVLKINTFTAVKNLHANKLVLAGGVAANSRLR
                     QVFQEQADYYQIELIIPSLKYCTDNAAMIARTGQEMLSRNQISPFSISAVPNLTLTTK
                     "
     gene            7579..9702
                     /locus_tag="PKX09_02650"
     CDS             7579..9702
                     /locus_tag="PKX09_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16755.1"
                     /translation="MHSLTLKIPGFEWWWFILLLGLVGLLFFSFRKIPGGRSRSVTTI
                     LLVLRSLILILLAGLILKPEIHWTSSRKKAPSALVWIDNSLSIAAQNNFSKDSLLSIV
                     QEINTALDGKGIKPHLFLFDEKIIPVTGKLKNIENNGLATDLAQVLQYSKTEFESENI
                     VGALIISDGVITRGEDPTFSDIEMPFPVFTIGIGDSELVMDPAVTKIEMPQTVKAGDT
                     VTINAEIIPTGNGDPITVMLKEGEIILDKKVIASQSQAMRKMLSFQAIPSKPGEKIYS
                     VEITAAQDKNPYNNLRMNAVKVLESQIKILIVSGQANFEAPFLTRTLRGLQDVFVQNV
                     VINKGQWLPLSFQEIMAKEWNLIILIGYPTAESSQNDLIRLRQKISDYNLPLIFFLDG
                     DVDSKQLERLLGWNPMNELLVDKSVNQISVKPTRAGLEHPLIKNFQKVNVSEIIWREL
                     PPIGSPYRRIRLKPSLLTIIESTDIAENPVIAVNQERNKPLALCIGLDFWRWSFMTNE
                     IGRFDIYDELFQGMTKYLTDTLSSSPIQLSINKRIFLNGESVEMSCLLYDLKGLVVND
                     AVVKAELIQQNDVIASSNLIWNGNNYSGVIPVRNAGDCQVRISAWSGDNLIGIREQPI
                     TVVDRSIELLEVRQNVDLLRTIALRSGGLKTNRIPEIIDRLQVTEKIITQSHVIKFLN
                     WRWTLVILIVLLTVEWSMRRFQGFL"
     gene            9863..11176
                     /locus_tag="PKX09_02655"
     CDS             9863..11176
                     /locus_tag="PKX09_02655"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOU16756.1"
                     /translation="MKKIAMLGVGYVGLVSGTGLADFGNEVICYDIDTTKIEKLNNGE
                     MPIYEPGLIDLVRKNVAKGRLKFTDDLTLAIRESEVIFLAVPTPSGENGEVDLSYIFN
                     AVDQIAKVIDSFKVIVTKSTVEVGTNDQIRERLLSHKIAPEMFEVVSNPEFLREGSAV
                     SDFMRPDRIVIGTSSPKDLEIMKEIYRPLYLIETPIVATDIHTAEMIKYASNAFLATK
                     ISFINEIANLCELVGADVQQVAKAMGLDGRIGKKFLHAGPGYGGSCFPKDTLALTKIA
                     EKAGMRATIVEAVIEVNNRQKLRVVEKLLKVLPNLSDKTIGILGLAFKPNTNDVRETP
                     ALTVIEEILKRGGQIKAYDPEAMKEMRKYYPQITYVASTEDAAKGADALIIMTEWNEF
                     RNLDLNYLKSVMKNPIMIDSRNIYDPQKVQAVGFIYDCIGRKIVN"
     gene            11191..12210
                     /gene="rfbB"
                     /locus_tag="PKX09_02660"
     CDS             11191..12210
                     /gene="rfbB"
                     /locus_tag="PKX09_02660"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOU16757.1"
                     /translation="MSDSERKVYLVTGGAGFIGSNYIHYLFSKYKNVRVINLDKLTYA
                     GNLANLQDLADNSDYIFVKGDICDADTVNNIFRKYHPDYVVNFAAESHVDRSIGKPDD
                     FIRTDMFGVFVLLEASKEFGIERFIQISTDEVYGSILEGSFTEESPLKPGNPYSASKA
                     GGDRLAYSYFNTYNLPVIITRASNNYGPYQYPEKLIPLFVTNLIEDKPVPLYGDGKNV
                     RDWLYVWDHCDAIEFILKNGKPGEVYNIAGENELENIFITNFIFEYMNKPKTLIKPVA
                     DRLGHDRRYSISAAKLHELGWQAQNKFEDAMRNTIQWYIDNPQWWKPLKSGEYLEYYK
                     RYYKI"
     gene            12222..13460
                     /locus_tag="PKX09_02665"
     CDS             12222..13460
                     /locus_tag="PKX09_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOU16758.1"
                     /translation="MKKAFVILMVAILFDISLGQQYRSSESQKSYRSYGKMFEQETEK
                     EKTTLKEKAKEVSKMQELIALDKAVSPEEYIVGPGDIFGIDIIITENINIELAITPTG
                     DLLIPSVGKINVNGLILKDAIELCKKKINEVYPSAKVDVALVNLRTFKIQIAGAVFQP
                     GFYDVTAVTRLNEIIELAEGFDQFAKEFNIQITRNDGRIDTINYFNYKLEGDLKHNPT
                     FLEGDRIFVPFGDPDKEAIVIRGAVYGSGYDIIQENESLGYYLRRQAQFIENADLENV
                     TITRKEADHQLVMTVYPRDFFSTPLKPGDAIDILSERGISVNGFVQSPGSFYFYPGYS
                     YLDYINMAGGNTYEGDIDKVYIRHLDGSTEPGKSATLKRGDVIIIPRSSKSTIFGDLS
                     VLEIFTSIATILLTFIAAMK"
     gene            13499..14491
                     /locus_tag="PKX09_02670"
     CDS             13499..14491
                     /locus_tag="PKX09_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOU16759.1"
                     /translation="MAPIKKEHPLLYFIFKHRVTNLLILLLGAILVFVIVFFGMKKKY
                     TATVSILPPSQDLSSGIGSSMGALANLAGINLPQSTLQSPEMYKEILTSRKVLDTILF
                     EEFEGVDSSGTIYRKKLIDMLDIKAKNEREKIDKALKKLLKDVIYVNINNESNVTKLS
                     VTLTDPFYAANVANRMVELLNEIVVTQIQHENRQQLLYIEDHIKNTQDSIKIAEKYLQ
                     TFLEQVKSIAEPANQIKELALKRDLELYSTIWIELKQQKEIYTLKSIPSLAEVKVLDI
                     AQAPYLKSRPKRLLTMISLGGLFLILQICVNALILIINNFRKELKPDEPIPHPK"
     gene            14466..15773
                     /locus_tag="PKX09_02675"
     CDS             14466..15773
                     /locus_tag="PKX09_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HOU16760.1"
                     /translation="MNQFPTLNELKGFFSNKEGKSLFANFAQFGIYQVIIYLAPLITV
                     PYLLRYIGVAKFGIISFAMALVYYFQIVVEYGFGITGIQMIAQSQNNSRKQSEIVSNV
                     LIIQIILFSICFILLGILCFLIPKIHDYRSIYLFSYGYVLANIFLFTWFYIGVEKVKY
                     VNVIISISKLLYILLVIIFIKNENDFVLVPIFNSGTTFLAGIVSIIILRKKFIIRFLK
                     PNKDILKRYLKEGWHIFLSNIAMNLYRNTNVIILGIFSSDISVGIYSAGEKIVKAIQS
                     TLSPLTQTLYPFISRKKIEEPEKSIRYISRMITIMGFIGCIISILLFIFAGKIAYLLN
                     GSVNLAMVRVIRIASFVILFGGLNYCIGIIFMTNYDMKKEFLKSVMIVGILNTIICAI
                     LCHLISEIGAATALLLSEFILFMLLVYHIQHNKYKWSTNPLEL"
     gene            15770..16834
                     /locus_tag="PKX09_02680"
     CDS             15770..16834
                     /locus_tag="PKX09_02680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU16761.1"
                     /translation="MSYPHVSIIILNWNKWADTIECLESVLKSDYPNFNIILVDNGSD
                     NDSVEKILAWADGKLIKPYISNYDEIVLPEVKKPLIIQKIQIENINHVPTESGKNAMI
                     NLLISNENLGFAKANNLSIRYADATWNSKYYYLLNNDTVIEKETLRNLVTVLENETLY
                     KIAQSTIFYYNNKEEIACAGGRLFPWGKGKFYTRITSDEIKEIGFINGCALLISQEIV
                     EKYGLLSERFFFGEEDFEYSYRLSLAKVKKVCVGNSRVYHKISVSSNHLLDNDRGRSL
                     VLSALNRIADMRNYYPRFIWIFWRYLTVIYYYLFLMIIKHGIDTKLANKYLKYILKKS
                     RNISSVDKTLYEEIINKKWN"
     gene            16825..18099
                     /locus_tag="PKX09_02685"
     CDS             16825..18099
                     /locus_tag="PKX09_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6337 family protein"
                     /protein_id="HOU16762.1"
                     /translation="MELTKLSSLFFAAVILAMTIFEKRRLNTIITPFSVSAWPLVFII
                     LVVNFMLVKIEFDIVTPRAILFLLANLFIIWLIGIIFSYIHNNDRIQSLNDIFLPYVK
                     FEKILIVLSWVVNIVVFLKMKNLLAKYGGLSFLGDYRFEEMINSGPEAHISQFGKVTF
                     LLLSFIYPMANHKKTILITLFGSIVMLFMLQSKYNLIGVLVIVFIYYNFTKPVKKQIG
                     SILKFSLLTLMIMALSLITLTIFWGTFKVSKQSVWLFLSKNLLSYFFSSPIVLDKWLD
                     HAFVQPDWTLLLLFKNIINRIVGNPLVYSSLDYVDLNFSEVAKDVYSNVGTSYGTYFI
                     IGGWIFSLFMSTVISSLTYFVYYSAKKKPNPVNVFLCMTLLNIALFSFFGQYFTFLPY
                     YEMIIFYIVTIKVMDTYNLFISDYQNSLPLRK"
     gene            18099..19208
                     /locus_tag="PKX09_02690"
     CDS             18099..19208
                     /locus_tag="PKX09_02690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU16763.1"
                     /translation="MFRKIKILHVVNVFGGAVGRILVDVMPHLVKKFDVKVVSIQSID
                     YSEPLVQEYIRKYNNLESLKLNRFSLFKAYHKLNCYFKKIDPDLIHSHMGRADIFTAM
                     SKTKNMIIMNTLHCEKKNHNPLTQIGYRLTDRRVDWRICISQTVARSYYNHGFKSPYS
                     VIYNPIDINGFTKPINREEIRNNLGFKPNEQIIINVGRLIKCKGQRYLIEVMPHLCHL
                     LPEIKLLIVGDGPQYNSLKKLIINNNLENNVYLLGYRQDVQNLLKISDLFVYSAQWEG
                     LGMAVIEAMATRIPVLAHPLPALKEYIIDNHNGYFCNIHLKESFANKIYDILTNSHHN
                     SSIAENAFRTVTDMFSADKISKQYIELYQQLLSNR"
     gene            19251..20360
                     /locus_tag="PKX09_02695"
     CDS             19251..20360
                     /locus_tag="PKX09_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU16764.1"
                     /translation="MLVSNTSWYLYNYRRPLIIEMINRNINVIAVTPKDDYTDLLKKI
                     GVNHIDIKINRKGMNPITDIILFLNLIKIYHCQNAICIQHYTIKPVIYGSLAAKVLHF
                     KNVINVIPGLGYVFGNKSNHQKIIKILVKCLYRHTLGVSKVCMFQNPSDRDYFVKSKI
                     IQISKTEVTYGSGVDLNYFDFNKCVEKEYGHCTFLLFGRLLRDKGVLEYVESARILKR
                     KYPNAIFNILGFIDDGNPEAILFNEIEKWKKEKYINYLGNTDDVRPYIINSDVIVLPS
                     YYNEGVPKSLIEALSIGRPLITTDRPGCREVINDGKNGIIIQPKNVEQLVNAIEYMIQ
                     NPDERIRMGIESRLYAEIKFDVNKVNEIFFKYMNI"
     gene            20548..21735
                     /locus_tag="PKX09_02700"
     CDS             20548..21735
                     /locus_tag="PKX09_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOU16765.1"
                     /translation="MLKINLMSNKLIKSIYLIWINYGSKLLHKYYHFLKRYKRDGFGM
                     YVRILKEDENIGYNNFIAESRFSSIYQSFEWGIVKQYDHWEVIRLIVIDSGQIVGGLS
                     CLCKKSPFFAKRIFYIPRGPVLDYFAENAPDIFKLLFLKIREIARREGALYIRISPDI
                     SKNIHFNDYISKYNLHRVRYPILHTTTYRLDLTKSEEQLLNSMESRVKYDIRKAQREN
                     VEILSDDGNEEYLKDFYNLLYQVAERNKFPIYSYEMMQNIWNILSPNGMCKIFLARSQ
                     GKIISGAFLFLFGDKCIYQWGGSVRAKNNPNQLLHWEIIRWAKSNGFSIYDLQGIPEN
                     VKKGDELWGIYLFKRGFGGDRIQLIGEYDYILSPILYKIWQVIEPKYPAVKNCLSKIK
                     KRK"
     gene            21705..22547
                     /locus_tag="PKX09_02705"
     CDS             21705..22547
                     /locus_tag="PKX09_02705"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOU16766.1"
                     /translation="MFIKNKEEEMSKRLILAYHRILPDELALLRQPLAVSTTQFERQI
                     LYLINKGWRSLTLSEYFEIYYLEQKKPDRVFVVTFDDGYRDNYYYAFPILKKYNLKAT
                     IFLVTGLLNEPKTLQSDNNINNNLTEMDYSITLEQMREMANYGIEFGSHTVTHPRLDT
                     IGLAEAEEEIYQSKRMLEQYYNRQVKTFCYPYGAFNDDVISLVAKSGYQCAVVTPTRP
                     GIKETRFTLIRVGVYNSNSLLKFKFKCSNLFTNIRKARLWFLLKHLSIYLKKKSTIKT
                     SFVW"
     gene            22792..25335
                     /locus_tag="PKX09_02710"
     CDS             22792..25335
                     /locus_tag="PKX09_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HOU16767.1"
                     /translation="MALASLLAVFLTTYICALGLTYLARKIALRYHIGDMPSERKVHK
                     VFTPYLGGAAILLSIIIGIVLSIYLLPMVAEEVPRRYYFLGITGVLIALVGLYDDIKK
                     LRFSTKFYFQILAAIIVVLGGLRFESLYIPIIGEVGLGWFSIIFTILWIVFITNAMNL
                     LDGLDGLAAGVSTIIFLAFALVALHSGRTLVALVCLLFIAANLGFLKFNSHPASIFMG
                     DTGSLFIGFSTAVISLEVGRIGHTNCLDIIIPLTILAVPILDTTISFFRRINKHMNPF
                     MADKEHIHHRLMSIGFSHKNAVKVMYFLSIIAGFIGCSFIYLDNRAIVTILFAGAIFV
                     GLVIRRLGYFEIEKNLIVVPNGTNNNSKTNGFSNGHSEPRFIPFDTSQFIQSFIFFLS
                     DVLFIVGSFLAVYYWQIVPNLLPEQLLTPYNLILWIAWSVIYWTSLLGLNNLYRIEWD
                     TSRVDEIFTVFKIIFFGTIVLFFLSFEFKIPFLPSRRILVTYGIFLVTGISVGRLVLI
                     TILKKYNLLEFKKRPTLIIGASRRALNVVRKINSVPELKFNIVGMVDDHANGNIGKII
                     EGVPIIGSFENIPEIIKKRKIKEVIIALDDNNMNQVIDMIDLFSRYNVSIKLLPDFYN
                     LLTGFRTSHIYGVSLVKFFGSNMKTWEWVLKRLIDIIISLVVLIGFLPIWLIISLIIK
                     LDSPGPVFYKQKRIGKNRREFEVIKFRSMVQGAEQETGPKWAEKNDPRVTRFGKFLRK
                     TGLDEIPQFFNVLMGDMSIVGPRPERDYFVNDLERSIQFYSRRLIIKPGITGWAQIKY
                     KYDETIDDVREKLRYDLYYIENMSVILDLKIIIQTLLVGLRRKHRTSYV"
     gene            25428..26705
                     /gene="serS"
                     /locus_tag="PKX09_02715"
     CDS             25428..26705
                     /gene="serS"
                     /locus_tag="PKX09_02715"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOU16768.1"
                     /translation="MLDIKQIRNEPEKFRRGLEAKNAGDKLDELLKWDGERRKCINQA
                     DELKAIRNRVSDEIGQLKKKGLPVAEKILEMRSVGDQINNLDHKLKEIDEKINSILIT
                     LPNLPHTSVKIGKSAADNVTIKEWGHKPEFDFPLKDHLDLGEALGILDMKRAAKISGS
                     GFPMYIGDGATLERALVNYMLDFHRNHHGYQEVFPPFMVNRDSAFGTGQLPKLEEDMY
                     HIEIEDLFLIPTAEVPVTNIHRDEILPENRLPIKYTAYSACFRREAGSYGKDTRGLSR
                     VHQFNKVELVWFTTPENSYQVHEQILRDAEDILQALNLHYRVVELCSADLSFAAAKCY
                     DIEVWAPASGKYFEVSSVSNFEDFQTRRANIRYRRTSDNKVNFVHTLNGSGVATPRLM
                     IALLETCQTPEGTIIIPQVLQKYTGFNQIRTKK"
     gene            26702..27358
                     /locus_tag="PKX09_02720"
     CDS             26702..27358
                     /locus_tag="PKX09_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOU16769.1"
                     /translation="MTGEKKYRKLICEIGKKIYANGYVAANDGNLSMRLNAEMVLITP
                     TNVSKGAVKPSQIAKIDLSGNPLDKNRPSSESLLHLAIYKRCDWVKAVIHSHPPYATA
                     WAVIGLGLTDPILPETIVSIGQIPLIPYKPPSTQELADIVADAAQKNDVLLLQNHGLV
                     AVGKDLLDAYYKTERAEHVFRIVALAKQIGEIRTLSKAEVAELYRLFNVSEKLQVTYR
                     "
     gene            27366..28079
                     /locus_tag="PKX09_02725"
     CDS             27366..28079
                     /locus_tag="PKX09_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOU16770.1"
                     /translation="MIRNFFAVIWVVLVTIVFGSLSMALPGSHNTMMRKWSQMILQFL
                     GVKLQVHGLENIVNRGSKIVISNHESALDIPILMAALPVPIRFMAKKELFQIPLFGWT
                     LNLARHIPVDRKRPRKSYTVINRVAKELIRNGLSIAVSPEGTRSYDGKIAPFKKGAFR
                     LAHIHNLPILPVLILGARHCVPNKSLSIIPGIVEVHIGKEVHLSDYPTMNDCVADLRN
                     QMIVQKEQYEEKRKQGSLD"
     gene            28027..29427
                     /locus_tag="PKX09_02730"
     CDS             28027..29427
                     /locus_tag="PKX09_02730"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOU16771.1"
                     /translation="MSKRNNTKKKESKVALINCIPNRIAEPEISITKKEKSLYKAWLN
                     QEFNLADLQTVEGYSLSILSPGNRNETEGPDFHDALIMLNEKFERGDVELHLESNDWY
                     SHGHDRDPHYNNVILHVVLNSGKLLPIKSCDNRIIPTLIMTPFAISAPDEYACLSWKH
                     LSNDDLNQVLKTFAEIRFKRKAQIWRSLILTKGAEQTLYEGLADVLGYSRNRKMFGIL
                     SQKVTLELIRKILRDTDPEQRIIVLESLLFGSAGFLTKPELKVFIRDTKYSEGLYKIW
                     VKISQKYELEELESADWHFAGSRPPNFPTLRIAALAQIINKFYPNYLAETWLRVIKNA
                     KIFTDIRRYASEMFQQPDGLWRNHPLLKQQPGKMLIGVQRFNDLLSNLLLPFSWAIAS
                     LDNQYELIEKTTTFAEQVECLEVPGSVKVALNRLPLDTITLPRNYLIQGIIEFSRRYC
                     NMNICKLCPLEKYAQK"
     gene            29414..29893
                     /gene="rfaE2"
                     /locus_tag="PKX09_02735"
     CDS             29414..29893
                     /gene="rfaE2"
                     /locus_tag="PKX09_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOU16772.1"
                     /translation="MPKNDKIITREQAGRIVSQLKREGRKVAFTNGCFDLLHIGHLEL
                     LEHARKLGDILIVGLNSDDSVRRLKGPQRPINTQLDRARILAALEVVDYVVIFNEDTP
                     LELIQTIKPDFLIKGGDYSPDTIVGSAFVESYGGKVIVFPLVHGKSTSDLINTIFQL"
     gene            30008..31771
                     /locus_tag="PKX09_02740"
     CDS             30008..31771
                     /locus_tag="PKX09_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU16773.1"
                     /translation="MTITISVALSANNSYEKINNNNGEEKELSDNSGNDIFELIFNEA
                     KLFYVDALVSSYYNDTAAVKYCFDRIFEIIAEISELDSLTLVEQDDFNRFNEMVSNDY
                     QTHFSYLIEETDSVMIVSVGEELFVTPLDTVDIGNDTLIVVEDRPGHIPIVRSKKIDK
                     LIIYYSGQQAPVIQRYLEAFNKYKDHYLPILKKYNVPEEIIYLPILESGYNPNAYSYA
                     HAAGIWQFIAGTGAKYGLKRNWYVDERRDPIKSAHAAAKYLRKLYEEFDDWYLALAAY
                     NCGEGNVWRAIRREGTRDYFKLRSLPSQTRNYVPSFMAITIIAKNPEKYGLTTPTTPT
                     WEWEEVIIDRCYELDVIAKACETTPDILRQYNPELRRWMTPANDNQYVLRVPKGKAEG
                     LTEKLVSLPEPKDVKPEVVYHKVRKGENLSSIARKYGTSVSALVSANHLRNRNQLRVG
                     QVLLIPTGSYYSAPEKKSSQTNVVTHVVQRGETLSEIAEKYNTSVSKIRSMNNIHNSH
                     IQVGQKLKVPTNLASQIEPSDNPKGSTKVIHVVKKGDTLTSIANKYNVSLTKLKSWND
                     LDGRKPIYPGQRIVIYQSIKS"
     gene            31899..32795
                     /locus_tag="PKX09_02745"
     CDS             31899..32795
                     /locus_tag="PKX09_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HOU16774.1"
                     /translation="MNSINISLMSNVGHLILTMVSSEILNTERHRDGVKYFCKLFRIE
                     SSLPSINHVTSILRKFSLLPYENISKILSLNQNWDENHFRWPQEIICDYEKYKLGGTC
                     FSLTFFLKAILDYFGYQTTILMADMKAGRNTHCALLLEFKNRKYLLDPGYLLHFPLPL
                     NSAVISEKVQLEYDPFCERYSLSTLENGKKKWRYSFQERPTSIEDFSQYWEDSFHWMT
                     MHGICLSKRDENNFIYLHNHYLKLENRSQHFKGNLNTEIAELAQRWFNIPPEIVRQAE
                     KALRENLYQDKILGYQIPKWFK"
     gene            complement(32932..33798)
                     /locus_tag="PKX09_02750"
     CDS             complement(32932..33798)
                     /locus_tag="PKX09_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOU16775.1"
                     /translation="MTNYSSSSRKSPFISNEVLRRYFDEIRTEKTLTPDEEIELARKV
                     KAGDTQALDRLLKANLRFVVSVAKKYQGYGLSLADLINEGNIGLIKAARRFDETRGFK
                     FISYAVWWIRQTILQAISENSRLVRLPLNVVGSLNKITKITSEFERDYEREPTNDEIE
                     VLLQNEKIDIDSARQLTENTISIDAPVGNDQTGTLQDILPSQEDNDPGLTLNQESFHL
                     EIERALNSLDKREAYILRLYFGIDSDLPLNLEDIAERLHLTRERVRQIKEKALRKLRH
                     NSRALHLRGYLG"
     gene            complement(33826..34218)
                     /locus_tag="PKX09_02755"
     CDS             complement(33826..34218)
                     /locus_tag="PKX09_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16776.1"
                     /translation="MTGIWDDIKKSVGGFASKAAEKAGELSREAAEKAEEMTQLGKIK
                     LDIFQIKRDIDKNYTELGRVVYGLLNQNTKGNLVKNEKVTSLINNIKELEKKLKDKEA
                     QYYKIQESSKENTAKPDEPDQSKSETET"
     gene            complement(34351..36030)
                     /gene="groL"
                     /locus_tag="PKX09_02760"
     CDS             complement(34351..36030)
                     /gene="groL"
                     /locus_tag="PKX09_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561033.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOU16777.1"
                     /translation="MAKDITYEIQARTALKEGVDKLANVVKVTLGPKGRNVVIEKKFG
                     SPTITKDGVTVAKEVELEDKLENIGAQMVKEVASKTSDVAGDGTTTATVLAQSIIAEG
                     IKNVTAGADPMELKRGIDTAVNTVVESLKELSKAVTNSKEEIAQVGTISANNRKVRRM
                     IKDESGKEDIVEIPIGVLIAEAMEKVGKEGVITVEEAKSTLTTLDLVEGMQFDRGYIS
                     AYFVTNAEAMEAVLEDPYLLIYDKKISSMKELLPILEKVSQMGRSLLIIAEDVEGEAL
                     ATLIVNKLRGTLKVAAVKAPGFGDRRKEMLEDIAVLTGGKVISEEAGYKLENATISHL
                     GNAKRIVVDKDNTTIVEGAGKPEDIKGRIKQIKAQIEASTSDYDKEKLQERLAKLSGG
                     VAVLKVGAATEVEMKELKALVEDALHATRAAVEEGIVPGGGVALIRCIPALDKIKVTG
                     DQMVGVKIIKRALEEPLRQIAKNAGHEPSIVAQKIKEGKDDFGFDAYKEEYVHMYKAG
                     IIDPTKVTRIALQNAASIAGLMLTTEAVITEVPEKEPAAPAMPPGGMGDMY"
     gene            complement(36052..36336)
                     /gene="groES"
                     /locus_tag="PKX09_02765"
     CDS             complement(36052..36336)
                     /gene="groES"
                     /locus_tag="PKX09_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOU16778.1"
                     /translation="MAIKPLADRVLVKPLVSNEKTPGGIYLPDTAKEKPQEGTVIAVG
                     PGKTTDDGKKIPLEVKVGDHVLYGKYSGTEVMIDGEEHLIMRESDIFAIL"
     gene            36587..37366
                     /locus_tag="PKX09_02770"
     CDS             36587..37366
                     /locus_tag="PKX09_02770"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOU16779.1"
                     /translation="MISSNFLFILVGFVAGVLSGLFGIGGGIVIVPIMIWFCHFSQLA
                     ANSISLTALLLPVGILAVLAYHKSKLVKIRQALFISLGIFIGVVGGAILALEIPAHFL
                     KRLYGIFLLYIAWTYIDIPSLFANGANQEKTNQQFENLNLDRPVCYIVLGFAAGIMSG
                     LFGIGGGIIIVPALISIFKYPYKKAVGTSLMALLLPVGLPGVLIYNSAGQLQIGPALF
                     IALGLLFGALVGALVAIELPTKLIKRIYGGFLLLAGFNFIF"
     gene            complement(37409..37756)
                     /gene="rplS"
                     /locus_tag="PKX09_02775"
     CDS             complement(37409..37756)
                     /gene="rplS"
                     /locus_tag="PKX09_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002833579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOU16780.1"
                     /translation="MDKLTAAVADQFRTDIPEFKPGDTVAVEVKIIEGDKERLQTFQG
                     VVIARHGSGISETFTVRKISNGIGVERIFPLHSPKISKIKRISVGKVRRAKLYYLRGL
                     RGKASKIEESNRR"
     gene            complement(37749..38459)
                     /gene="trmD"
                     /locus_tag="PKX09_02780"
     CDS             complement(37749..38459)
                     /gene="trmD"
                     /locus_tag="PKX09_02780"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008403309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOU16781.1"
                     /translation="MKIYIITAFPGMITACLAESMFRKAVENGKVEFNVWNLRDFTRD
                     KHKQIDDKPYGGGAGMVLKPEPFFRAYDKIVKLTGHKRPRVVFPTPQGRLFNHHLALE
                     LAQESDLTFFCGHYKGVDERVIANLVTDEISIGDYVLTGGELPALIIIDAVIRHIAGV
                     LHSYESAETDSFTDNLLEGPIYTHPQVYRRKSVPEVLTTGNHAKINAWRQEQRKLRTR
                     QRRIDLIKNMNMEDKNNG"
     gene            complement(38456..38962)
                     /gene="rimM"
                     /locus_tag="PKX09_02785"
     CDS             complement(38456..38962)
                     /gene="rimM"
                     /locus_tag="PKX09_02785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOU16782.1"
                     /translation="MDHLVLLPVGSILKSFGLNGAVLLNRGSLKNIKVDEPKVLWLGE
                     SAQVARPWKVDYLRISTKKVYLKLRDINSRQEADYLRGLKVFVPREYELKKELVELIG
                     FRVRVINSAKDIGFISSLDETNPQALFVIQSEDATLLVPAVTDLIEQIDPEQQIVIFK
                     DIEGLFPK"
     gene            complement(38971..39204)
                     /locus_tag="PKX09_02790"
     CDS             complement(38971..39204)
                     /locus_tag="PKX09_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOU16783.1"
                     /translation="MKEFVEFIVKQLVDHPEDVKVVQVDSEKTVILELSVKEGDLGKV
                     IGKKGRTARAIRTLLTAAAAKQGQKRAILEIIE"
     gene            complement(39310..39918)
                     /gene="rpsP"
                     /locus_tag="PKX09_02795"
     CDS             complement(39310..39918)
                     /gene="rpsP"
                     /locus_tag="PKX09_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOU16784.1"
                     /translation="MAVKIRLQRMGRKKKPFYWIVAMDSKCRRDGRYLDKLGYYDPLT
                     KPATVKIDKEKVFSWLEKGAVPSDTVFNLLQKEGIAMEWHLIRNNISEQVRNIEIQKW
                     QLAHNKSAEIAEEVKEAQAVEVPEPESVPVVENVEEELTPETKPAEKAETPGKPKSAA
                     AKKAKAIASAEEKVIPEIELSEETSETSSEVTESKTDVQTEM"
     gene            complement(39936..41261)
                     /gene="ffh"
                     /locus_tag="PKX09_02800"
     CDS             complement(39936..41261)
                     /gene="ffh"
                     /locus_tag="PKX09_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOU16785.1"
                     /translation="MFEQLQNGFGAIIKSLRGEGRITENNIADAMRQIRRVLLEADVN
                     YKVAKEFVQSVQEKSVGVTVAKSLSPGQLIVKIIYDEMVHLLGDKNRNLKEASIPPTI
                     IMLVGLQGSGKTTCAAKLANQLLQKQHSPVLVPVDVYRPAAEEQLEILARQIKVPCFR
                     ENPDVVGRIQDAITFARQQHRDTLIIDTAGRLHIDEKMMAELKTIKNTIKPHNILLVV
                     DGMTGQDAVNTATSFNEQIGVDGIILTKMDSDTRGGAALTIVSSTGKPIIYMSVGEKI
                     GDLEIFHPERVAGRILGMGDVVSLVEKVQASVDLESALKLENKLKKEQFTLEDYLTQL
                     RQLKKLGPLDSVMEHLPGFARMRLGNQKVDEKELIKAEAIIQSMTRTERLKPNIINGS
                     RRRRIALGSGTQPQDVNRLLNQYWQITKLMKQMGRMKLPKNLSAFGIGN"
     gene            41462..42484
                     /locus_tag="PKX09_02805"
     CDS             41462..42484
                     /locus_tag="PKX09_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HOU16786.1"
                     /translation="MNRRHLMIVLTFPIFLMYNCSETQPESSNPYRINSGLNPGINIG
                     NALEAPNEGEWGVVIKDEYFPLIKSAGFNSVRLPIRWSAHADINPPYTISDDFMNRVR
                     HIVDTALEHKLYVVINVQHFQEIYTEPIENKPRLLAFWHQIGLAFKDYPQTLLFEPLN
                     EPHFNLTPELWNEWIPELIDTIRVTNPNRTLVIGTADWGGIWKMKELKLPTTERNLIL
                     AIHYYEPFRFTHQGAEWEKESNAWLGRTWTKTTDQMQELNQHFDLIKKFADSLGVPVY
                     IGEYGCYNRAPEESRLIWTRTVSEQCEKYGFSRAYWEFCSSFGIYDPISQQWREPLRS
                     AVLLAR"
     gene            42760..46677
                     /gene="porU"
                     /locus_tag="PKX09_02810"
     CDS             42760..46677
                     /gene="porU"
                     /locus_tag="PKX09_02810"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOU16787.1"
                     /translation="MIINKRFCAKIIFTSFCLFSAMLVRLEAVELTILSETTSELILR
                     VEMDADLEWRQIENYPNKVSALTLGDAYQSHLSQTLEIPYYQWLIALPTEDKPSVSIT
                     KSVFESVKLLKNLTTDELTALDKFPLVAIAETGFLRDVPFGSLVIYPLRSANNSNELR
                     LLREIEITIQYTAVRKTTINQTTVEKGDYLNAFVNGSSATQWQQSRPLSLRKPQSQPA
                     GKWFRLTVKKDGVYTLALDDLKSAGLTETSIDTGRIYLYSNSTGGRPLDATIGAAVPS
                     NLIENTRWIKGNNDQDFTNADTILFYGRATSGVDVDSKNNLTFVRNPYSLINYYWLFI
                     ADEPGYPKAMPTINSLSATPNMTVTQSEHLEVHEEEKVNFLRSGLKWSGEKFNTSGSN
                     VSVILTMPKVRDSYVGVMTICTRGAVDEILHYFKAYLNNSTTPVASWSATNYQSATTS
                     FSTTLSANINILKINYTSQPSSASAYLDYVMFRYTKDLSAFTEPQTFWSLLYSGIVEY
                     QITDLNFTNLLILDVSDWSNVSIQQYNRNSNLISFRTASQSNQRRQYYLTAPSFFNKP
                     EKIELIPNPQWITLRNITSSYDYIIITADDFLTAAEKIATLYSQELSPGDRLNTLVVT
                     QNQILREFNADITDPHAIRFFLKYAYENWTPAPAYALLLGDGTFDYRAIESSTGNYVM
                     TYQVEPAVEDYSGFSSYASDARFVYIKGSDQVMDMAIGRVPVRTATEALDYYEKLKQY
                     LTEPIYGEWRSRITLVADDPQRPYNNEKYHINDTESYIAKYIPKSLITDKLYLLEYPE
                     VQDVSSNWVKKPAATEAILNQLKRGTTLINYLGHGSSTVWAQEEVLKMDRDIGLIEAG
                     MKLPLWIAGTCSWGQFDDINTICMPEALINKNSDGAIAILTPTRPTTGTQNASFIGNF
                     IKKLFPSGEISRTRLGTLIQNIYNGSIENQEKYVLFGDPALYLALPYQKAAFDKMPRD
                     TLAALEKVSVTGRTVTEQNFNGDGIINVFDSDRYVTRYYLDDAKVTQNLSYTLSGETL
                     YRGNLKIENSKFFSRFYIPKDLSYMNLFGRIKVYGWNTSAMSDFAGCYDSLLFTGTTQ
                     FDDTSGPQIVIGFEGMDFKSGDIVAPDAKIKVSIMDPVGINIAGKLGHDITFRFDNDN
                     DLIFEMTEDFVYDVNSDSSGVITSDLPDLSPGIHQITVKAWDNANNYATAIAEFVLAS
                     SDKLKLERVVNFPNPFASTTDFVFYLTQPGQVKITIYTIRGLKIKTIASDEILPPGFH
                     QVNWDGCDDFGDEIARGIYLYKVQVQSSDSNQKDTFIGKMVKS"
     gene            46674..47273
                     /locus_tag="PKX09_02815"
     CDS             46674..47273
                     /locus_tag="PKX09_02815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HOU16788.1"
                     /translation="MTTQLNWYSMPVVLASQSPRRKEILDMIGLKYEVCPPHYIENNS
                     IGKSPTTIVIKHALNKAQTVAPRYPKAWIIGADTVVVCKNRILGKPQNENEAVQMLKF
                     LSGVEHSVFTGYCVLNSANGRYLKNAVRTRVRFKEIPLAEIQYYVENYHPLDKAGAYA
                     IQDFSAIFVEKINGCFYNVVGFPLPAFYEQVKNELSICL"
     gene            47341..>47911
                     /locus_tag="PKX09_02820"
     CDS             47341..>47911
                     /locus_tag="PKX09_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU16789.1"
                     /translation="MATFYEILKQSREAQGLTVEQISSRTKISKEIITAIESGDLKIL
                     PKTYMRLFLKAYSQELNLDPEAILKNFEESLGEPVETPAVPDNNIKPSLPISQKPEKP
                     EKPIIGKKQNRNFSTVIIVFVILVFLIAVLRQVLMDEKKTSSNTALPKITPTDTTQTI
                     PPSPDLEVAVPKSELNLTITTKDSCWVRIV"
     assembly_gap    47912..48011
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            48166..48618
                     /gene="mraZ"
                     /locus_tag="PKX09_02825"
     CDS             48166..48618
                     /gene="mraZ"
                     /locus_tag="PKX09_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOU16790.1"
                     /translation="MITAFAGETRHPLDEKNRLSIPAKYRRWADGQDGECTFVVTKGS
                     DPCLVVYPSTEWDVLSEKLLKLSYFKKKNRAFIRALARSSVRLKCDGQGRILIPTSLL
                     EYAHIKKEVTIIGVLNRLELWDPETLEKHEESSIPLDDEYYEDLGDIL"
     gene            48643..>49517
                     /gene="rsmH"
                     /locus_tag="PKX09_02830"
     CDS             48643..>49517
                     /gene="rsmH"
                     /locus_tag="PKX09_02830"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOU16791.1"
                     /translation="MTTLHIPVLVEQTIQFLINRPTGIYVDCTLGTGGHFQAIAQHLD
                     NDAYLIGIDADPSAVQYCEQNISISQPHQFITANFADIKRICYRAGFPKVDGILMDLG
                     LSSFALDNPERGFTYSQAGPLDMRFSPDTAQTAMDFINTAPMKELALVFKEYGEERYS
                     YNIARRIITERDKNPIKTTGQLASIIREEVPKQFQIKTLSRIFQAIRIQVNQELDALR
                     QGLSDAVSLLAPSGRIVVISYHSIEDRIVKQFFKKEASDCICPPDFPICQCNHHAQLK
                     ILTHRPIVPSKSEIE"
     assembly_gap    49518..49617
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            49653..>51028
                     /locus_tag="PKX09_02835"
     CDS             49653..>51028
                     /locus_tag="PKX09_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOU16792.1"
                     /translation="MANQFKSIRKRTLIVSSSLVVLWVVLIARLFYIQVLNSKKYQEL
                     CNNQANLRKAVLPRRGIFYDRNGEALTIDLVNYSIAAHPYLVEDKPRLASTLANDLSA
                     KPEQYLKLLESNKTFVWLEREVPYSSFQNYEKYEKTPGLVIEKNVKRYYPLGEITGQL
                     LGFTNIDNRGVCGLESGLEGLLCGTPGWIMVQKDGWGRLHYRPDLPSKSSIDGNDIVL
                     TIDREYQTILYEELLQAYTETKAEKAMGIVINPNDGNILAMVTLPPFNSNKPSDYPIS
                     AQRNAVVSDIFEPGSIFKIVTATAALDNKVLQPKHQIDCNPGYIQVGNRIIRDHDKYS
                     ILTFAEVIKNSSNVGTIKVAQLTGKDQIFNYARKFGFGAKTDIQFPGEESGILHPFKK
                     WNDLVLAQVAIGQGVCVTALQTGMAYAAIANGGLLLKPQIVKTIKTKDGIEIFNAQPV
                     YIRRVASS"
     assembly_gap    51029..51128
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <51129..51521
                     /locus_tag="PKX09_02840"
     CDS             <51129..51521
                     /locus_tag="PKX09_02840"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOU16793.1"
                     /translation="GGGVAAPVVREVFKRIVNQSDDLFFDEPAPLPTPQANYAYETKP
                     VVANVPQTSDEAYHPNLTTAIYTNQMPDLCGKTLRQAVGILYELGIDDIEIDGAGVVV
                     SQKPAPHAPINPHVKCILKLQPTRTPLE"
     gene            51514..52977
                     /locus_tag="PKX09_02845"
     CDS             51514..52977
                     /locus_tag="PKX09_02845"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOU16794.1"
                     /translation="MNNSLSEILQGIEFRRLGNLPSSVAALSVNSKELAKGALFFAIR
                     GAKDDGHKYVKNAIQNGAAAVVVEEVVNDESIPQIIVADTKTALSKAAANFYENPTNN
                     MTIVGVTGTNGKTTTVYLLNEIFKEAGLKHGTVGTLGYSIGDERFSSNLTTPDSIQLQ
                     QIFKKMLNRGVEIAAMEVSSHALALHRVDDVHFKAAVFTNISQDHLDFHHSLDEYATT
                     KSKLFAMIEKDGFVINNLDDNYSDLFKNAARCRLFTYSLLKTADFTWAPGVTWSNGIS
                     GEILTPNGKIEIKSRLSGQFNLKNILAATAVAVNLNIDDKIIASALDRVKSVPGRLQE
                     ISHPGQFRVFIDYAHTPDAITNVLQTLRGMLPADGRLIVVFGCGGNRDRDKRPKMARA
                     VESLADLAIVTTDNPRFEEPHDIINDIVKGFTSGYNFKVIIDRKEAILDALKNAEQND
                     IIAILGKGHETYQEIRGVRYPFYDGEIVQQFYGGSND"
     gene            52970..54319
                     /gene="murF"
                     /locus_tag="PKX09_02850"
     CDS             52970..54319
                     /gene="murF"
                     /locus_tag="PKX09_02850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOU16795.1"
                     /translation="MIKADFIRSLGLGRIVNFEGAINGAEIDSRKLEPGAAFFALKGA
                     TSDGHAYVQEALQKGAVLCVVAEDWAAKNPTPAPLWIVPDPEKALQKLAEKWRLSFSI
                     PVLAITGTNGKTTTRAMCAAIIKTAYNLHTTSGNFNNQLGLPITLLNLRPEHQFSLIE
                     MGTNHYGEIARLCEIAHPNAGLITNVGWGHIEFFGNIEGVAQAKSELFAALPENGTAF
                     INADDPLIVKMPVAGRKIKYGFENKAVDFYGKISGYNDEGQAVLNVNSRFDIHLSIPG
                     RIAALNALAAVAVGLFFNVDEKAAIAALEAFQPVSQRFVQIKVGPYQVINDAYNANPD
                     STIAALETFGRIKTSGRRIFIMGDMLELGQYSSSGHQRVGQAVAKVGIDCFYGVGTQT
                     AYATEAAAQVGLKNVYHFQNKSDLLSALKSDLKKGDTLLVKGSRGSRMEEIIEGLSQ"
     gene            54321..55424
                     /gene="mraY"
                     /locus_tag="PKX09_02855"
     CDS             54321..55424
                     /gene="mraY"
                     /locus_tag="PKX09_02855"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOU16796.1"
                     /translation="MFYHLLYPLKKYFTGFNLFQYITFRAAMAAITALLISFIIGPYI
                     IKKLKKYQIGELIREDGPQTHLAKKGTPTMGGIIILFSVIVPVLLWANLTNTYINLIL
                     LSTVWMGIFGFLDDYLKVVKKKKKGLIARYKLLGQIALGLLIGCYLYFSPEFADINSI
                     TSVPFLKNNYLDFGWFYIPFVIFFITGISNAVNLTDGLDGLAAGLMGIVALALAVIAY
                     ITGRVDFSNYLNTIYLAGSGELSIFCMAAAGAFLGFLWFNAKPAEVFMGDTGSLSMGS
                     ALATLAILLKKEILFIFLGALFIAEALSVIIQVVYFKWTKKRTGVGQRVFKMAPLHHH
                     FEMQGIPESKIVIRFWIIGILLALFSLSTFKIL"
     gene            55421..56815
                     /gene="murD"
                     /locus_tag="PKX09_02860"
     CDS             55421..56815
                     /gene="murD"
                     /locus_tag="PKX09_02860"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOU16797.1"
                     /translation="MIDLKTKPANLNDLKVAVLGAKRSGIAAAVLAARRGAEVLLSDT
                     ANPEIAPEQHSALKQHNVQLEIGGHTSKIFDSDLVVLSPGIPNDAPIVIEVTKHSIPI
                     VAEVEMAYWFCPTDNIIAVTGSNGKTTTTTLLSEIFKGTQYEPYCGGNIGTAFSHLII
                     EAERSAHPQKIFILELSSFQLERIVHFRPKVSLILNITPDHLDRYEHRMELYLAAKLR
                     ITMNQTEDDYYIFNDDDELLKTNLPPRCHLIPAGLHSTSQKLITSDAEKIYFSGQDQF
                     IERARVALLGEHNLYNLTAALTAGRLYQLPITHLQNVLYNFKSIEHRLEFVTTIDGVD
                     YYNDSKATNVDSVNYALKSFNRPVIVILGGKDKDSDFTLLIPQIREHVKAAVLIGKAA
                     DKIRKALIGVVEMYDAGYSMPNAIEIARNLAVPGDIVLLSPACASFDMFNDYEHRGRV
                     FKEIVMNLPKDLKV"
     gene            56812..57954
                     /gene="ftsW"
                     /locus_tag="PKX09_02865"
     CDS             56812..57954
                     /gene="ftsW"
                     /locus_tag="PKX09_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOU16798.1"
                     /translation="MNVAFKKGKVDQKYLIAFFILLGFGLVMQYSASSSLGEARFDDP
                     SFYVRGQFIRIVLGMVVGLICTFINYQILKKGAFWIALAGIIALIATLVYHKLHPGQA
                     TARWFPIGGFNFQPSEFAKLALIIYLSSFIERHERHLDDFKRGFIPPVIIMLIMVILI
                     IVEPNYSTAAVFTVFGLIVLFIGGTKLLHFLPFIGGFALLSVITILRSPYKLMRILTF
                     LEPEKDLQGAGYQIHQSMITLGNGGFFGRGLGGSIEKNLFLPDMHTDFIFSVVGEELG
                     FIGAVVLLSLYLYLFIRGIKIALRAPDVFGNLLGIGLSSCLFIYVIVNVGVTCGLLPV
                     TGLPLPFISYGGSSMLFNFACVGIILNISRYAVPQEKTNSLVMFND"
     gene            57947..59086
                     /gene="murG"
                     /locus_tag="PKX09_02870"
     CDS             57947..59086
                     /gene="murG"
                     /locus_tag="PKX09_02870"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOU16799.1"
                     /translation="MTKKDESRRLKVLITGGGTGGHLFPAIALAEELQMRQSQIGELE
                     IIFIGTRRGLESQIVPSLGFEFHKIWIRGWQRGWTLNDILVNLLFPVRLFVSLIQSFF
                     IIKNFDPDLAIGTGGYTAGPPLRIAAMLKIPIFLHEQNVIPGATTKMLANKAKRLYTS
                     FEESKTYLENAICLGTPLRRSLQIVPREQAIYFFELEPDRKTVFIFGGSQGSQAINSY
                     WIENITTVVEAVKCQFIWQTGQRDFKMIDNIFSNNPWVHVTPFIHEMGIAYCAADVVI
                     SRAGALSLAELCLYGKPSILVPLPTAAGNHQEINARNMEQRGASILVLQKELTNDRLT
                     QELIALLKDEPRLQAMSANAKALARPDSAKLIIDDILGFIEVNAL"
     gene            59076..60434
                     /gene="murC"
                     /locus_tag="PKX09_02875"
     CDS             59076..60434
                     /gene="murC"
                     /locus_tag="PKX09_02875"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOU16800.1"
                     /translation="MHSEVKKYHFVGIGGIGMCGLAEYLLHNGQIVTGSDLAPSENTE
                     RLENLGAQIKYGHRRENVDDCDYLVYSSAVKPDNPERMVAIERQIPTLRRAELLGQIF
                     DTKPIRIAVSGTHGKTTTTAMIGQVLTTAELDPLILSGGVLKSSGSPVQIGKGDIVIA
                     EADEFDRSFLQLSPTHAIITNIESEHLDCYANLTQIKNAFYEFTAKIPDAGRVIACSD
                     EKEIPEFLKHHPRPYVTYGLYSGEFQARNIQLKGNSSECEVYHNSDLIGSLQLHVPGL
                     HNIKNGLAAIAMASLLNIPFPVIQEGLADFRGVKRRFEILQENDNLMLVDDYAHHPSE
                     IAATLNAARKGWQRRIIAVFQPHLFSRTRDFYPEFALVLRVADVIVLLEIYPAREAPL
                     EGISSRLIYDEIRKDRTQDVYLTNTLDEALALLQEIKLTGDMIITLGAGDVNKILLKL
                     ME"
     gene            60744..61556
                     /locus_tag="PKX09_02880"
     CDS             60744..61556
                     /locus_tag="PKX09_02880"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOU16801.1"
                     /translation="MIDEMTVEKATKISRNDQTKRPRKKVWFFLCLALIGGFSYLVYL
                     AVDWCEYTNFFNLDKIEVSGVSILSNEEIIKIAGLKNHVSLLKLDIHQIQNRLEMEPY
                     IEAAVVSREFPNHLKIMIKERIPVCYLNTGTLWLIDQQGIILPLRKEKLKANLPVITG
                     FEKDSARCRPGQLAPNPEMFKALNFIQTAALKANTLYTEISEIFAWKKGEFIIYTVNG
                     GTPIYIGRGDLTKQLYILAAFQNLIKSKRTFADYQYLDLRWDKQIIARERKS"
     gene            61557..62828
                     /gene="ftsA"
                     /locus_tag="PKX09_02885"
     CDS             61557..62828
                     /gene="ftsA"
                     /locus_tag="PKX09_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOU16802.1"
                     /translation="MKMLKLKNQILTGLDVGSSKVCAVIAEVTPDSPEPIIKGYGISP
                     SKGIKKGLVVDIENATQSIVNAVSEAEKMAGFSINSCLVGVSGDHIRSLNTRGMMAIS
                     RDSRTGLGEAREIEIEDIERVIEHTKAVPLPLDRQILHVLPQEYIIDEQPGVKNPLNL
                     SGRRLDAKVHLTTYNTTVLSNLSKCIKNADLEIDGFVLSSLAAAYACLEESEKTMGSV
                     LLDIGSGVIDIIVFYDDGVHHTGVVNLGANSVTNDIAYMLRVPLEKAESIKREYGFAK
                     VSLTDREAFFRLDSLGGRSPREISIFNLAEWIEPRMEEILRESYLEAKKADIPLTNTL
                     SVVLTGGGARLKGTDELAEMIFNTPARVAAPRGFQEYVTELSDPIYSVAIGLIKYAIA
                     GQISGSALNKTISGPFHRIRGWIKHVTENVM"
     gene            62844..64043
                     /gene="ftsZ"
                     /locus_tag="PKX09_02890"
     CDS             62844..64043
                     /gene="ftsZ"
                     /locus_tag="PKX09_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOU16803.1"
                     /translation="MLFEFEELVNQQARLKVVGIGGGGGNAITRMIENGLIGVDFIAL
                     NTDVQALECCKAPTKFQIGRALTRGLGAGAIDDIGRKAAEADRQTIASMIEDTDMVFI
                     TAGMGGGTGTGAAPIVAQIAREQGSLTVGIVTKPFNFEGAKRMARAEEGINQLRKCVD
                     TLIVIPNQKLLAIVDHDTSILDVFKTADSVLYQAIRGISDLINKNGVINLDFADVRTI
                     MKDMGKAIMGTGLATGEERAVLAAQQAISSPLLDEVNIKDAKGLLINITGPANLDIHE
                     VDEACSIITDEVGANAETIFGMVVDENYTDEIMITVIATGFGEAPTPINLSQNKKDFS
                     SPIKDEPRNTDDIWNRINEPGQPVSLFSDDQVIKILNPDDMGMADNPSHKIHQTNIPT
                     YIRQKIK"
     gene            complement(64133..64327)
                     /gene="rpmB"
                     /locus_tag="PKX09_02895"
     CDS             complement(64133..64327)
                     /gene="rpmB"
                     /locus_tag="PKX09_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOU16804.1"
                     /translation="MSKVCDICGKKPIHGNKVSHANNRTHRIWEPNLRKIRIVDNSGS
                     IKTVKVCLTCLKSGTIQKAV"
BASE COUNT        19491 a        12531 c        12783 g        19243 t
ORIGIN      
        1 aattccaaaa ttaaaaaagc tatcactgat ccccagaaaa taagacgcgg cgatcccggt
       61 cgtcccgaaa tttgtctggt ttatacctat catgaaaaat ttaatcctgc ccaatgtccc
      121 gaaatttacg ccggttgcac aagcggcgct ctcggttgtg tcgaatgtaa aatgaaatgc
      181 ggaaaggcga ttcaaagtgt tatcgcacct tatcgtactc gccggatgta ttatgccgct
      241 caccaatctg aggtcattga cattttaatt gatggcgaga aacgggctcg tcaggttgcc
      301 caaaacacta tggctgatgt ccataataaa atgagaatcg gataagacgt gataatccaa
      361 tacctggact ggcgaatcca gcaattttaa tcggattaat gagttacaac gtcaaactaa
      421 acgtatatga agggccactc gacctcttgc ttttctttat taagagagat gagattaaca
      481 tttacgacat acccattgcc tatataacgg atgaatatct taattatcta caactcatgc
      541 agagtttaaa tctgcagtta gcgggtgaat ttatcctgat ggcttccatc cttataagaa
      601 tcaaagttca aatgctcctg ccgcgtccgg aaaccgaaaa actcgaggat attgaagatc
      661 cacgcaccga attagtccaa atgcttgtcg aatatcagaa atacaaagag atcggcgagc
      721 aactgaagcg acttgaagca aatcaatccc gccattttcc agtcacactg acgtcagacc
      781 agcgcaatta tgatcccgaa cttttcctcg gtgatataac tttgatcgat ttggggcttg
      841 cctttcagca aattctcgag aaactgccaa aagttgagcc ggtcaatctc gaaattatga
      901 aggtcaacat tcgtcagcaa atattattca taagagaatg tttaaaaaaa agaagccgta
      961 ttcgattttc cgaaattgcg gctaaattaa ctgaccgact cgaagttgtg gttacttttc
     1021 tggctattct ggaaatgatc aaatcaatgg aaattagcgt atcgcagaag gatattttcg
     1081 gtgaaatatg gataaccaag aaaaaaacta ttcaggataa tgttatctca aacgcataga
     1141 atcgtactag cgcttttatt cggcgcaacc gagccgttga ctaaatcaca ggtagatatt
     1201 tgtctcgaag aggacattga tttgacacaa attatcgccg atttaaatca atatcttgaa
     1261 acggaaggac ttccggtctg gattcagttg gtggcgggag gttatcggct aatgacaaga
     1321 gctgaatacg aaccctggct aagtcgactt tttcaaaata agggcaaatt acgtctgtca
     1381 cgctcggctc tggaaaccct agctattatt gcttacaaac aaccggttac caaaagcgaa
     1441 attgatgcga ttcgcggggt tgataccagt ctcagacctc ttgtagaaaa aaatctgatt
     1501 aaaattaccg gtcgtcaaga aggacctggg cgacctctgc tatatggtac cactaaccat
     1561 tttttagaat atttcggatt ggcatctttg caagatttac cacaggttaa agaactggcc
     1621 gaaattgccg gtgaagacaa tctccaatct gatattgtcg aacaggatgc gactgaatag
     1681 atatttagcg cagggaggtt ttagttcacg acgcaaatgt gaagatttga tccgaggagg
     1741 tcgggttaaa ataaatggat gtatcgtaac caacctggct actcaggtaa aagaaaccga
     1801 tcaagtgact gttgacaatc gccgaattgt tccacctaaa ccagtgtata ttctcttaaa
     1861 caaaccagct ggctatataa caacaatgca cgaacaattc ggccgcaaag cagtcaccga
     1921 tttagtaaat aatgccttcg ccaaacctat tggtcgtttg gatatggcta caaccggagt
     1981 gcttctgctg actaatgacg gcgaattagc ctaccggctg actcacccca agtttggtgt
     2041 tgaacgcatc tatcgagcag caattgctgg tagagtctct cccgatatta aaaaacaggt
     2101 agcggaagga atttccatcg gaggggcgca aaaagcatct ggtcaaatcc ttactataaa
     2161 atatttagat ggtttttcca gcgtcaccat cgaattaaag gaaggtcaga atcgcgaagt
     2221 gcgtcgcatt ttcgctgttt taggctataa agtcatttca ctggatcgga taagtttcgc
     2281 cggtgtgcgc tattcaggat tggaacgtgg tcaatggcgc aatctaaatg aatctgaagt
     2341 acggcgactg aagactctgg ttggtttggc agataaatga cggagtgaca aatttttaca
     2401 attgattatt caaatattgt attaatttac cttaagatag ctgaaaggaa tttgaattgt
     2461 tatcctatgg aattaaattt acgcgctttt tgaaaggtag aatatgggca ttataatagc
     2521 aatcgatgga ccggccggta gcggaaaaag taccactgcg cgggaagtag ctcgccgact
     2581 gaatttcact tatattgata ccggcgcaat gtatcgagcg ataaccttgg cagttatccg
     2641 gcaaaatgtt gcagtcgaag atcatcaatc tgtgataaaa attgccgaac aagccgacat
     2701 tcagtttgag tgggcggatg gcaatttgcg tacgatttta aacggtgagg atgtaagtaa
     2761 tgaaatccgt tcagctcaag tcgcgcgttt ggtaagtccg gttagtgcca ttggaggcgt
     2821 acgtgacatt ttagtcaagc gccagcgcga aatggctaag aaggcaaata ttgttatgga
     2881 aggtcgcgat atcggcacca atgttttccc cgatgctgat tttaaatttt atttagaagc
     2941 tgatttggaa acgagagctc gccgacgaat taacgactat caaaagatcg gccagaattt
     3001 atctataacg gaaattatga ctgagatcga acaaagagat aaaatcgatt cttcgcgcga
     3061 acattcacct cttaaaaaag cccctgatgc tattgtaatt gacacctcca atctaaaatt
     3121 tgaagaacag gttgagatga ttatacaaat tgtgaaatca tctgaactct tcaaaaagat
     3181 agaaaaggct ccggtcatta acggaaccaa ttgtaaaaat taaccctaaa ccaaaggaac
     3241 attttcaatg ttagaagaag aacaaacctt aaaccctaaa ccctccgaag aatcggaagt
     3301 aattccggac attaacacgg ctgatccatt cgccgagcca gtgactgcca gagatgccac
     3361 gattgaggaa agaacatccg ccacttcgga ttatactacc gaattagtca cttcggggcc
     3421 attgtcagaa attgtaagag tatatgattt aaaagatatt gataaattga atgaagtcca
     3481 ggctcccaag gccgaagacc atacaaaaat ctatgaaaac tcactaaaag atattgcaaa
     3541 agatcagcgc atactcggaa cagttgtagc cattaatgaa aatgaaattt tagttgatgt
     3601 cggcttcaaa tccgaaggta ttattacccg tgaagaattt gaagaaggtg aattgccgaa
     3661 aatcggcgac cagattgaag tatttgtaga tgtacttgaa gacgaaaatg gtcagatgat
     3721 tctttccaag cggaaagccg attttatgcg aatctgggaa cgaattaagg aaatttatgc
     3781 ggctggtgaa actgtagaag ggcaaattgt cagccgtatt aaaggtggaa tggtagtgga
     3841 tattatggga attgatgcct ttctgcccgg ctcacaaatt gatattcgac cggttaccga
     3901 ttttgattcc tatatcggga aaacttttga atttcggatt gtcaaattaa atgaattacg
     3961 gaaaaatatt gtcttgagcc gtaaagaatt actcgaagaa agtatgaagg aaaaacgtga
     4021 tgatttatta tccaaaatca aagtcggtga tattctaact ggccgggtta aaaatataac
     4081 cgattttgga gtttttattg atctcggtgg attagacggc ttactccata tcaccgatat
     4141 ttcctgggga cgtatcaatc acccgagaga agtagttcag atggatcagg aattgacagt
     4201 caaagtcatt gattacgatc aagaaaaaca gcgtgtttcg cttggactca aacaactgac
     4261 cacccatccc tgggaaggta ttgaaagcaa atttcctgtt gggacgatcg ttaaaggcaa
     4321 agttgttaat atcactaatt atggcgtatt cgtggaactc gaaaaagggg ttgaaggact
     4381 aatccatatc tcggaaatat cttggactca gcatattaaa catccttcag aggtgttcaa
     4441 attgggcgat gaaatcgagg ctaaagtgct gggcatagac agtgccgaac ggaaaatttc
     4501 attaggatat aaacagcttg aaccagaccc gtgggaatca attgaacaga aattcacagt
     4561 gggttctgtt cacaaaggta tagttagaaa tctgactcct tatggcgcct ttatcgaact
     4621 tcaagaaggt gttgacggat ttgcccatat ttcagatatg tcctggacga aaaaaatccg
     4681 ccatccccgg gaattgctaa aaaaagacga tgaagtcgaa tttaagattc tggaaatctc
     4741 gaaggaaaat cgcaaaatat ctctcggttt aaaacaggtt actgaagatc cctggccaat
     4801 gattgaaact cttttttccg tcggaagtat agtggaaggc gaagtcgcta aagtgactga
     4861 taaattcatt gtggtcaatc tagaacacga tctcgaagga ataattccat taggacaaat
     4921 gcctaaaaaa gatcgtaaag acctcagcaa ggcagttgaa attggtgaaa aactgcagct
     4981 caaagtcttg gaagtcaacc gacaagaaaa gaaaatcata ctatcacgtg aacaggctat
     5041 tccgcgcgaa cccaagaccg aagtagaagc ttatatgatg caacaggctg aaacaactaa
     5101 caaactcgaa atcccgagtg aaataattca gaaaatcgcc gaatcggaag aaaaaactca
     5161 ggaaaaagaa gaaaagtcag ccgcaaaggc taaatctccc gccaaaaaag ccaagaaagc
     5221 ggaaaatgtc gaaaaaccag aagatctcac cacagaagaa gaaaagccca agaaaaaaag
     5281 taccaaaaaa gtcatttctg aagaaatacc tgctgaggaa aaacctgaag tagaaaccaa
     5341 aaaagaaacc gaagacagtc cggaaaagac caggaaaaat tcggccaaat cgacttctaa
     5401 aaaatctaaa ccgcaggccg aaagtgaaaa accagccgct gacgataccg ctgaaaaata
     5461 aggagcctga aatcgtttaa aaataacaat ggggcttgga ttttgacagc cccattgtta
     5521 ttttgcaaag ttgtatgaat ccaagtctta aagaaaaacg ccgcaaattt aatgagaaat
     5581 tgtcctatcg gaatgtcagg caagccgtcc gaaaatggat taccaccaat acgcgtacta
     5641 agatttggat cttcgttttc accgtttttc tctggctcta cgtcgtattg aataatactt
     5701 attcttatac tttttcagct aaactggaag tgagaaatat tgacccgact aaaacgacga
     5761 ttgagaaatt accgtcgcaa gtccaagcta gtttttcagg taaaggaatt gacctgtttt
     5821 atcttatcct atcacctcaa caatcaatca aatttgtcgt agattgctac tccattaaac
     5881 aatattacga tttcccttta aatgaatact ttaaaaataa tcccgaaaaa gtcatcactc
     5941 cacgaaatat taatgtcaaa ctcgatcaca ttatctggcc ggaaaccttg cgcgttgttt
     6001 tggacgattt agtcacggca aaagtaccgg tcaaaccgga ggttgaaata caattagcac
     6061 ctggctatgt cttagccggt tcaatgcgta taattccaga cagcgttact ttaaggggtc
     6121 cgcgaactta tgtcagacaa tataatgaag tgccaactaa aaagctggtt ctcactaatg
     6181 tttccagttc gatttcgcag gaaattgatt tagaatttgt ccctaagtat aatgtttata
     6241 ttgatgtaac gcgcgtccgc atcctgcaga aggtcgacca attgggtgaa aaaaaattaa
     6301 ccgacatccc aattagagta atcaatcttg ctcaagatca gaaagccgaa attattcctc
     6361 caacagccac attgactatt tccggtggaa tcgaaaaatt gaaaacagtt cagccggaag
     6421 atattaaaat cgtatttgat tttgccacag attggcgacc ggatgagtcc ttttacactc
     6481 caaccgtaga actgccaccc ggactcttaa actggtggaa tctcgaaccc gatacttttg
     6541 aaattcggat aattcgcgag aggcaaccct gaatgctaat tctcggcatc gaaacatcct
     6601 gcgacgaaac cgccgttgcc attttaaatg ataatgaaat tttagtcaat ctggtctctt
     6661 ctcaattact tcatcagcag ttcggaggcg ttgtcccgga atttgcttct cgtgaacata
     6721 tgcgtctgct tgatcagatg gtaaaagaag cattcgtcca gaccggattg caacccgaaa
     6781 atcttgatgg tattgctgtg actcgcggtc ccgggttaat gggagcttta ttggttggat
     6841 taagtttcgc aaaaggtttg gcatattcac ttcgtaaacc cttactgggt gtcaaccaca
     6901 tcgaaggtca tattatggct aattttttga cctgcccgga tttaaaattt ccctttctct
     6961 gtttactggt ttccggcggc catacccaaa ttatccaggt gaatgacttt ggtaattatc
     7021 agattcttgg gcgcagtgtt gatgatgccg ccggtgaggc ttttgacaaa gccgcccgca
     7081 ttctggaatt accttatccg ggaggtccac aaattgatca aatggcacat cgtggtgatc
     7141 ccaattttta ccaatttccg cgggctaaag ttcgctccga gcagtacgat ttcagtttca
     7201 gtggactaaa aaccgcgatg ttatatttag ttcgtcaacg aggccaaaaa tgggctcagg
     7261 aaaatttagc cgatttatgc gccgcttatc aggaagcgat cgttgatgtc ctgaaaatca
     7321 acactttcac tgcagtcaaa aatctgcacg ccaataaact ggtcttagcc ggtggcgttg
     7381 ccgctaattc cagactaaga caagttttcc aggaacaggc tgattactac caaatcgaat
     7441 tgataattcc atctttgaaa tattgtacgg acaatgccgc gatgatcgcc agaaccgggc
     7501 aggaaatgtt gtcacgcaac caaatttcgc ctttttcaat ctcagccgta ccgaatctaa
     7561 cacttactac taaataaaat gcattcattg accttgaaaa ttccgggatt tgaatggtgg
     7621 tggtttatcc ttttattggg attagttgga cttttattct tctccttcag gaaaattccc
     7681 ggcggaaggt cgcggtcagt aacgacgata ttgctggttt tacgcagttt aattttgatt
     7741 ctactggcag gattgattct taagccggaa attcattgga caagtagtcg caaaaaagcg
     7801 ccatcagctt tggtttggat tgacaattcg cttagcattg ccgctcaaaa taatttttct
     7861 aaagacagtt tgctttcaat cgtccaggaa attaatacag cactggatgg gaaaggaatc
     7921 aagccgcacc tttttctatt tgatgaaaaa attattcccg taacgggaaa actgaaaaat
     7981 atcgagaata atggcctcgc caccgactta gcccaagttt tacaatattc taagaccgag
     8041 tttgagagcg aaaatatcgt aggtgcgcta attatttccg acggtgtcat cacccgaggc
     8101 gaagacccaa cttttagcga tatcgaaatg ccgtttcccg tgtttactat tggaattggc
     8161 gatagcgaac tggttatgga tcctgccgtt acgaaaattg aaatgcccca aaccgtcaaa
     8221 gctggagata cagttactat caatgctgaa ataataccga ctggaaacgg cgatccgatt
     8281 actgtaatgc ttaaagaagg tgaaataatt cttgataaaa aagtcatagc gagtcaaagc
     8341 caagctatga gaaaaatgct gtcttttcaa gcaattccgt ccaaaccggg cgaaaaaata
     8401 tattctgttg aaattacagc cgcgcaggat aaaaatccct acaataatct caggatgaat
     8461 gcggtgaaag tgctcgaatc ccaaattaaa atactcattg tcagcgggca ggctaatttc
     8521 gaagcgcctt ttctgactcg cactctacgc ggattgcaag atgttttcgt tcagaatgta
     8581 gttataaata aaggacagtg gttgccgctc tcatttcaag agataatggc aaaagagtgg
     8641 aatttgataa ttcttatcgg ctatccgaca gccgaatcca gccagaacga tttaattcgt
     8701 ctccggcaaa aaatttccga ttacaatttg ccactaatat tttttctcga tggagatgtt
     8761 gattctaaac agttagaaag attgctgggc tggaatccga tgaatgaatt gttggttgat
     8821 aaatcagtaa atcagattag cgtcaaacca acgcgcgcag gtctggaaca tcctctaata
     8881 aaaaattttc aaaaagtaaa tgtttccgaa ataatctggc gggaattacc accgatcggc
     8941 tcgccttacc gacggattcg ccttaagccg tctttgctaa cgattattga aagcactgat
     9001 attgccgaaa atccggttat cgccgtcaat caggaaagaa ataaaccttt ggcgctctgt
     9061 atcggattag atttttggcg ctggtcattt atgaccaatg agataggacg gtttgatatt
     9121 tatgacgaac tgtttcaagg aatgacaaaa tatttaaccg ataccctcag ctcttctcca
     9181 attcaactgt caatcaataa aagaatattt ctgaacggtg aaagcgtcga aatgagttgc
     9241 ctgctgtacg atttgaaggg cttagtagta aacgatgccg tagtcaaagc cgaactaatt
     9301 caacaaaacg acgtgatcgc ctcaagcaat ttgatctgga atggaaataa ttattcggga
     9361 gttatacccg ttagaaatgc cggcgattgt caggtacgca tttcagcttg gagtggcgat
     9421 aatttaatcg gaattcgcga acagccgatc acggtagttg atcgctcgat cgaattactt
     9481 gaggttcggc aaaacgtgga tttactcaga acaattgcct tacggagtgg cggtttaaaa
     9541 acgaaccgga ttcccgaaat tattgatcgc ttacaagtaa cggaaaagat tatcacccag
     9601 tcacacgtta tcaagttttt aaactggcga tggacgcttg tgattttgat tgttttactt
     9661 acagtagaat ggagtatgcg acgttttcag ggctttctat aaaaagtctt gaccttttaa
     9721 ttgtttatgt tcaaatttcc accgtgcaga ctagaattca aaaaaagagg tatttcagca
     9781 agaattggta aatccgatct ggtcttagca cgtaaaacgc ttaccccatt tattaaatag
     9841 aaaaaaatac cggagtgatt ttatgaagaa aattgcaatg ctgggagtag gatatgtcgg
     9901 tttagtatcc ggaacgggac tggctgattt cggtaatgaa gtaatctgtt atgatattga
     9961 tacgactaaa atagaaaaac taaataacgg agaaatgcca atttatgaac cgggtttgat
    10021 tgatttggtt agaaagaacg tcgctaaagg ccgactgaag ttcaccgacg atctgacgct
    10081 ggcaatacgg gaatcggaag tgatttttct ggcggtaccg actccttccg gcgaaaatgg
    10141 tgaagttgat ctttcctaca tttttaatgc ggttgaccaa atcgccaagg ttattgacag
    10201 ttttaaagtt attgtcacta aaagtacagt cgaggttgga actaacgatc aaatccgcga
    10261 acgtctatta tcacataaaa ttgcgccgga aatgttcgaa gtggtttcaa atcccgaatt
    10321 tttacgcgag ggttcggcgg ttagcgattt tatgcggccg gatcggattg tgatcggcac
    10381 cagttcacca aaagacctcg agataatgaa ggaaatttat cgtcctctct atttaatcga
    10441 aacgccgatt gtggcaaccg atattcacac cgccgaaatg attaagtatg cttcaaatgc
    10501 ttttttagcg acgaaaattt catttattaa cgaaatagct aatctctgcg aactagtagg
    10561 cgccgatgtt cagcaagtcg ccaaggctat gggtctggac gggcggatcg gtaagaaatt
    10621 tctacatgct ggaccgggtt acggcggctc ttgcttccca aaagatacat tagcgttaac
    10681 taaaatagct gagaaagccg gaatgcgagc tacaattgtc gaagctgtta ttgaagtcaa
    10741 taatcgtcag aaattgcggg tcgtcgagaa gctcttgaaa gttctaccaa acctctccga
    10801 taaaacgatt ggaatcctgg gattagcctt caagcccaac actaacgatg tccgcgaaac
    10861 gccagcatta actgtaattg aagaaatttt gaaacgcggc gggcaaatca aagcctatga
    10921 tccggaagct atgaaagaaa tgcgcaaata ttatccccaa attacttatg tggcttccac
    10981 tgaagatgcc gctaaaggag ccgatgcttt gattattatg acggaatgga acgaatttcg
    11041 caatttggac ttaaattatc taaaatcagt gatgaaaaat ccgattatga ttgatagtcg
    11101 caatatttac gatcctcaaa aagtccaagc agtcggcttc atttacgatt gtatcggccg
    11161 taaaattgtc aattaattag gaatatacta atgtctgatt ccgaacgaaa agtttatctg
    11221 gtgaccggtg gcgccggatt tatcggcagt aactatatcc actatctatt ttcaaagtac
    11281 aaaaatgttc gggtgattaa tctcgataaa cttacatatg ccggcaacct ggcaaatctg
    11341 caagatctgg ctgacaattc cgattatatt tttgttaaag gcgatatttg tgatgcggat
    11401 actgtaaata atatttttcg taaatatcat ccggattacg tcgtgaattt tgcggccgag
    11461 tcgcatgtag atcgtagtat cggtaagccg gacgatttta ttcggacgga tatgtttggc
    11521 gtttttgttt tattggaagc ttccaaagaa tttgggattg agcgtttcat ccagattagt
    11581 acagatgaag tctatggctc aattttggaa ggttcattta ccgaagaatc gccattgaaa
    11641 ccgggtaatc cttattcagc cagcaaagcc ggtggcgatc gtctggcgta ttcctatttc
    11701 aatacttata acttgccggt aattattacg cgcgcttcga ataattacgg tccttaccaa
    11761 tacccggaaa aactcattcc gctgtttgtc acaaatttaa tcgaggataa acccgttccc
    11821 ttgtatggtg atggaaaaaa tgtgcgtgat tggttgtacg tctgggatca ttgtgatgcg
    11881 attgaattca ttctgaaaaa tggaaaaccc ggcgaagttt ataatatcgc cggagaaaac
    11941 gagttggaaa acatcttcat caccaacttt atttttgaat atatgaataa accgaagaca
    12001 ttgataaaac cagttgcgga ccgactggga catgaccggc gttactcgat ttcggcggct
    12061 aaactgcacg aactcggctg gcaagcacaa aacaaattcg aagatgctat gcgcaatact
    12121 atccagtggt atattgacaa tccgcaatgg tggaaaccat taaaaagcgg cgaataccta
    12181 gaatattata aaaggtatta taaaatctaa gaggtatatt aatgaaaaaa gcatttgtta
    12241 ttctaatggt ggctatttta ttcgatattt cattagggca acaatataga tcctccgaat
    12301 ctcaaaaatc gtatagatcc tatgggaaaa tgtttgaaca agagactgaa aaagaaaaaa
    12361 ctactttaaa agaaaaagca aaagaagttt caaaaatgca agaactaatt gctcttgata
    12421 aagcagtttc acccgaagaa tatatcgtag gccctggtga tatatttggg attgatataa
    12481 ttatcactga aaatataaac atagaattag ctataactcc tactggggat ttattaatac
    12541 catcggttgg aaaaattaat gtaaatggtt taattctgaa agatgccatc gaactatgta
    12601 aaaagaaaat caatgaagtc tacccaagtg ccaaagtcga tgtagcattg gtaaacttac
    12661 gcactttcaa gattcaaatt gccggcgccg tgtttcaacc agggttctat gatgttactg
    12721 cggttacaag attaaatgaa ataattgagc tggctgaggg atttgaccaa tttgccaagg
    12781 aatttaatat ccagataact agaaacgatg gcagaattga cacaataaat tactttaatt
    12841 ataaactcga gggagacctg aagcataatc ctactttttt agagggcgat agaatattcg
    12901 tcccatttgg agatccggat aaagaagcta tcgttattcg aggtgcagtg tatggaagtg
    12961 gctatgatat tatccaagaa aatgaatcct taggctatta tctacgacgc caagctcagt
    13021 ttattgaaaa cgctgactta gaaaatgtaa caattacgcg aaaagaggct gatcaccagc
    13081 tagtaatgac ggtatatccg cgagatttct tctccactcc attaaagcca ggtgatgcga
    13141 ttgacattct ctcagaaaga ggaatatcag ttaatggatt cgtccaatct ccaggtagtt
    13201 tttatttcta tccgggctat tcatatttgg attatattaa tatggctggt ggaaacacct
    13261 atgaaggaga tatcgataag gtttacatac gtcacttgga tggaagtacc gagccaggta
    13321 aatctgcaac tcttaaacga ggtgatgtta taattatccc tcgtagttct aagagtacaa
    13381 tattcggaga tttatcagtt ctggaaatat ttacttccat tgcaactatt ttattaactt
    13441 ttattgccgc aatgaagtaa atgaaattta aaattttcat aaataacagg agaattacgt
    13501 ggcacctata aaaaaagaac atccgcttct ctattttatt tttaaacatc gcgtcacaaa
    13561 cctattaatt ttactccttg gagctatcct agtttttgta atcgtatttt ttggaatgaa
    13621 gaaaaaatat accgctacag tgtcaatttt acctcccagc caggatttat catcaggtat
    13681 aggcagctcg atgggtgctt tggcaaattt agctgggata aatttacctc agagtacact
    13741 ccagagtcct gagatgtata aagaaatcct tacctcaaga aaagttttag atacaatcct
    13801 atttgaagaa tttgaaggag ttgattccag cggtacaatt tatcgaaaaa agttaattga
    13861 tatgcttgac atcaaagcca aaaatgaaag agaaaaaatt gacaaggcat tgaaaaaatt
    13921 actcaaagat gttatttatg taaatatcaa taatgaaagt aatgttacta agttatcggt
    13981 gacacttacc gatccttttt atgcggctaa tgtagccaat aggatggtag aactattaaa
    14041 tgaaattgtc gtaacccaaa tccagcatga aaatcgtcaa caattactat acattgaaga
    14101 tcacatcaaa aatacccaag acagtattaa aattgcagaa aaatatttac aaacattttt
    14161 agagcaggta aaatcaattg ctgagccagc taatcagatc aaagaattgg cattaaaaag
    14221 ggatctggaa ttgtattcta ctatctggat cgaacttaaa cagcagaaag aaatttatac
    14281 attaaagtca atcccaagcc tggcagaagt taaagtcctt gatatagccc aagcacctta
    14341 tttaaagagt aggcctaaaa gattactaac tatgatcagt ctgggagggc tctttctaat
    14401 tttacaaata tgtgtaaatg ctctcatttt aattattaat aattttcgca aagaactcaa
    14461 gcctgatgaa ccaattcccc accctaaatg agttaaaggg atttttctct aataaagaag
    14521 gtaaaagttt atttgctaat tttgctcaat ttggcatata tcaggttatt atttatttag
    14581 cgccattaat tactgttccg tatctcttga ggtatattgg agtagcaaaa tttggtatca
    14641 tcagctttgc aatggctctc gtctattatt ttcaaatagt cgtagagtat ggatttggga
    14701 taacgggaat ccagatgatt gcccagagtc agaataatag taggaaacaa tcagaaatag
    14761 tatcgaatgt tttaataatt caaattatac tattcagtat ttgttttata ctattaggga
    14821 tattatgttt tttgattcca aaaatacacg attatagatc catttatcta ttttcctacg
    14881 gctatgtctt agcgaatata tttttattta cctggtttta cattggagtg gaaaaagtca
    14941 aatacgttaa cgtaattatt tctatatcaa aattgttata tatactttta gttattattt
    15001 ttattaaaaa tgaaaacgat tttgttttag tgccgatatt taattcgggg actacctttc
    15061 tggcggggat tgttagcatt atcattttaa ggaaaaaatt catcatccgt ttcctcaagc
    15121 caaataagga tattttgaag agatatttaa aagaaggttg gcacattttt ctctcaaata
    15181 ttgctatgaa tttatatcgc aatactaatg ttattattct gggaatcttt tcatcagata
    15241 taagcgtggg aatatacagt gccggtgaaa agatagtcaa agcaatccaa agtaccttat
    15301 cacctcttac tcaaaccttg tatcctttta tctcccgaaa gaaaattgaa gaaccggaaa
    15361 aatccataag atatatcagt agaatgatta cgattatggg ttttattgga tgtattatct
    15421 caatattact atttatcttt gcagggaaaa ttgcttatct tttaaatggg tcagttaatc
    15481 tggcaatggt acgagtgatt agaatcgcct cttttgtaat tttattcggg ggtttgaatt
    15541 attgtattgg aataattttt atgacaaact acgatatgaa gaaagaattt ttaaagagcg
    15601 tgatgattgt gggaatattg aatactataa tttgcgcaat tctttgtcat ttaatcagcg
    15661 aaattggtgc tgcaactgct ctattgcttt ccgaatttat tctatttatg cttttggttt
    15721 accatattca acataataaa tataaatggt caacaaatcc cttagaatta tgagttatcc
    15781 acacgtatct atcataattt tgaactggaa taaatgggct gatacgattg agtgcctgga
    15841 gtcggtattg aaatcggatt atccgaattt caatattatt ctggttgata atggctcgga
    15901 taacgattca gttgaaaaaa tattagcatg ggctgatgga aaattaataa aaccttatat
    15961 tagtaattat gatgaaattg ttttgccgga agtaaagaag cctttaatta ttcagaaaat
    16021 acagattgag aatattaatc acgttccgac tgaatctggt aaaaacgcta tgattaatct
    16081 gttaataagc aatgagaacc ttggatttgc taaagctaat aacttaagca ttagatatgc
    16141 tgacgcaaca tggaatagta aatattatta tttattaaat aatgatacag tcatagagaa
    16201 ggaaacccta agaaatcttg taactgtatt agaaaacgaa actctatata aaatagctca
    16261 atcgaccata ttttattata acaataaaga agaaattgct tgtgcgggcg gtaggttgtt
    16321 tccttgggga aaaggtaaat tttatacccg gattacgtcg gacgagatca aagaaatagg
    16381 atttataaac ggatgcgctc tattaatttc gcaagaaatt gttgaaaaat atggtttact
    16441 tagcgaaagg tttttctttg gtgaagaaga ttttgagtat agctacagat tgtctttagc
    16501 aaaagtaaaa aaagtatgtg tcggaaatag tcgagtgtat cataaaatta gcgtctcttc
    16561 taatcactta ctagataatg atcgcggtcg tagcttagtt ttatcagcat taaacagaat
    16621 cgcagatatg cgaaattatt atcccagatt catctggata ttttggcgct atcttactgt
    16681 catttattat tatttattcc tcatgattat taaacatgga atcgatacta aattagccaa
    16741 taagtatttg aaatacattc tgaaaaaaag tagaaatatt agtagtgttg ataaaacatt
    16801 atacgaagag ataattaata aaaaatggaa ctgactaaat tatctagtct cttttttgcg
    16861 gctgttatat tagctatgac gatttttgag aagcgtcgct tgaacacgat aattacaccc
    16921 ttttccgtat cagcgtggcc tttagtgttt atcatactcg tagtaaattt tatgttagtg
    16981 aaaatcgaat ttgatattgt aacaccacga gcaatattat tcttattggc gaatttattt
    17041 attatatggc taattggcat aatattttca tatattcata ataacgatag gatacagtca
    17101 ctaaatgata tttttttacc gtatgttaaa tttgaaaaaa tccttatagt tctttcctgg
    17161 gtagtaaata ttgttgtatt tttaaagatg aagaaccttt tagctaaata tggcggttta
    17221 agttttttag gagattacag atttgaagaa atgataaaca gtggacctga agcacatata
    17281 tcacaatttg gaaaagtaac gtttctacta ctgagtttta tttatccaat ggcaaatcat
    17341 aaaaaaacta tccttattac actttttgga tcaattgtta tgttatttat gcttcagtca
    17401 aaatataatc ttattggagt tttagtaata gtatttatct attataattt tacaaaacca
    17461 gttaaaaagc agattggcag tattttgaaa ttcagtttat taacgttaat gataatggct
    17521 ctttccttaa tcacattgac catattctgg ggtactttta aagtatcaaa acaatcagta
    17581 tggctattct tatcaaaaaa cttactcagt tattttttct catctccgat tgttttggac
    17641 aaatggttag accatgcttt tgtgcaacct gattggacat tactattact atttaaaaat
    17701 ataataaacc gtatagtggg caatccatta gtatatagct cgttggatta cgtagactta
    17761 aacttttcag aagttgccaa ggatgtttat tcaaacgttg gaacaagcta tggaacgtat
    17821 tttatcattg gaggttggat ttttagttta tttatgagta cagtaatctc ttcattaacc
    17881 tattttgttt attacagcgc caagaaaaaa cctaatcctg tgaatgtatt tttatgcatg
    17941 actttattaa acattgcgct ttttagtttt tttgggcaat attttacatt tctaccatat
    18001 tatgaaatga ttatatttta tatagttacc ataaaggtaa tggataccta taacttattt
    18061 attagtgatt atcaaaattc gcttccattg cgaaagtaat gtttcggaaa attaaaatat
    18121 tacatgttgt gaatgtattt ggaggtgctg ttggccgaat attggttgac gtcatgccac
    18181 atttagtaaa aaaatttgat gttaaagtgg tatcgataca atcaattgat tattcggaac
    18241 cattggtaca ggaatatatt aggaaatata acaatctgga atcgcttaaa ttaaatcgtt
    18301 tttctctgtt taaagcatat cataagctaa attgctattt taaaaagata gatccagatt
    18361 taattcattc tcatatggga agagctgata tttttacagc tatgtcaaaa actaaaaata
    18421 tgataataat gaataccctc cattgcgaaa aaaagaacca taatccattg actcagatcg
    18481 gttatagact aactgataga agggtcgatt ggagaatttg tatttcgcag actgttgcga
    18541 gaagttatta taatcatggt tttaaaagtc cttattcagt aatctataat ccaattgata
    18601 ttaatgggtt taccaaacca ataaatcgag aggaaataag aaataaccta ggatttaagc
    18661 ctaacgagca aattataatt aatgtcggtc ggctaattaa atgtaaaggt cagaggtacc
    18721 ttatagaagt aatgccgcat ttatgtcatt tattaccgga aataaaactt ctcattgtgg
    18781 gagatggccc tcagtataat agtttgaaaa aattgattat taataataac ttagaaaata
    18841 atgtatattt attaggctac cggcaagatg tacaaaatct gttaaaaata tccgatctgt
    18901 ttgtttattc agctcagtgg gaaggcttag gtatggcagt aatagaagca atggcgacta
    18961 gaattccagt tctcgctcac cccctgcctg ccctcaaaga atatatcatt gataatcaca
    19021 atggttattt ttgcaatatt cacttaaaag aatcgttcgc caataaaatt tatgatatac
    19081 ttactaattc tcatcataat tcatcaatag ctgaaaatgc tttcagaacg gtgactgata
    19141 tgttttctgc tgataaaatt agtaaacaat acattgaact ttatcagcaa ttactatcaa
    19201 atagataatt aataggacta tagattaagg ataagagtat aaagacagtc attttagttt
    19261 caaataccag ttggtattta tataattacc gacgtccatt aataatagaa atgattaatc
    19321 gtaatattaa cgttattgct gttactccaa aggatgatta tactgatttg ctaaaaaaaa
    19381 taggcgttaa ccatattgat attaaaataa atagaaaagg gatgaatccg ataactgata
    19441 ttatactttt tctaaatctt attaaaattt atcattgtca aaacgctatt tgtatccaac
    19501 attatacaat aaaaccagtg atttatggaa gtctggccgc aaaggtttta cattttaaaa
    19561 atgtgataaa tgtgattcca ggtctaggat atgtatttgg taacaaatcc aatcatcaaa
    19621 aaattattaa aattctagta aaatgtctat atagacatac attaggcgta tcaaaggttt
    19681 gtatgtttca aaatccaagt gatcgtgact attttgttaa atcgaaaatt attcaaattt
    19741 caaaaacaga ggttacatat ggttcaggag tagatttaaa ttactttgat tttaacaaat
    19801 gtgtagaaaa agaatatggg cattgtacat ttttactttt tggacgttta ttacgcgata
    19861 aaggtgtgct ggaatacgtt gaatccgcaa gaattctaaa aagaaaatat cctaatgcca
    19921 tttttaatat tttaggattt attgatgacg gtaatccaga agcaatactt tttaatgaga
    19981 tagaaaagtg gaagaaagag aaatatatca attatcttgg taacactgat gatgtaagac
    20041 catatattat taattcagat gtcattgtat taccttctta ctataatgaa ggtgtcccaa
    20101 aatcgttaat agaagcatta tcaattggtc gtcccttaat tacgactgat cggcctggtt
    20161 gtagagaagt tatcaatgat gggaaaaatg gaataattat acaacccaaa aatgttgaac
    20221 aattagtaaa cgcaattgaa tatatgattc aaaatcctga tgagaggata agaatgggaa
    20281 tagaatcaag gttatacgca gaaattaaat ttgatgtaaa taaagtaaat gaaatatttt
    20341 ttaagtatat gaatatttag ttcacgtcta aacctgtata taacagtatt atgagaatat
    20401 gcaattacaa ttaatgttaa aattgtcgac caaatatata gagaagagaa tgaattttat
    20461 tattaacaat aacttgatat gaataatgga tttttatgat aaacaatccc gatatacggt
    20521 taaaaatggg aaaaatgtct aggctgattg ctcaaaataa atttgatgtc caacaaatta
    20581 ataaagagta tttatcttat ctggatcaac tatggtagta aattattaca taaatattat
    20641 cactttttaa agcgatataa aagggatggt tttggtatgt atgttcggat actcaaggaa
    20701 gatgagaata tcggatacaa taattttata gccgaatccc gtttctcaag tatatatcaa
    20761 tcttttgaat ggggaattgt caaacaatac gaccattggg aggttattcg actaattgtt
    20821 attgattcag gccagatagt tgggggactg tcttgtttat gcaagaaatc gccattcttt
    20881 gctaaacgaa tcttttatat tcctcgtggg ccggtattgg attattttgc ggaaaacgca
    20941 cctgatattt tcaaattact ttttttgaaa atacgggaaa ttgcacgccg tgagggggct
    21001 ttgtatatca gaataagtcc cgatattagt aaaaatattc attttaacga ttatatttca
    21061 aaatataatt tacaccgcgt ccgttacccg attttacata ccacgactta tcgcctcgat
    21121 cttacaaaat ctgaagaaca attgctgaac tcgatggaaa gtcgggtaaa atatgacata
    21181 aggaaagctc agcgtgaaaa tgtagaaatt ttatctgatg acggtaatga agaatattta
    21241 aaagacttct ataatttgtt atatcaggta gccgaacgaa ataaatttcc gatttactcg
    21301 tatgaaatga tgcaaaatat ctggaatatt ttgtctccta atggaatgtg taaaattttt
    21361 ctagctagaa gccaaggcaa aataatctcc ggcgcttttt tatttctttt cggtgataaa
    21421 tgtatttatc aatggggtgg ttcagtaagg gccaaaaata atcccaatca attattacat
    21481 tgggaaataa ttcggtgggc gaaatcaaac ggatttagca tatatgatct tcagggtatt
    21541 ccagaaaatg tcaaaaaagg cgatgaatta tggggcatat atttattcaa gcgcggattt
    21601 ggtggcgatc ggattcagtt aattggagaa tatgattata ttttatctcc gattttatac
    21661 aagatttggc aagtaattga accaaaatat ccagccgtga aaaattgttt atcaaaaata
    21721 aagaagagga aatgagtaag agattaattt tagcctatca tcggattctt cctgatgaat
    21781 tagcactcct aagacagcct ttggcagtaa gtaccacaca atttgagaga caaattctct
    21841 atttaataaa taaaggatgg cgaagtctaa ccttatcaga atattttgaa atatattatt
    21901 tagaacaaaa gaaacctgat agagtttttg tagtcacttt tgatgatggg taccgtgaca
    21961 attattatta tgcatttcca attttaaaaa aatacaatct taaagcgact atttttttag
    22021 tgactggact attaaacgag cctaaaactt tgcaatctga taataatatt aataacaatt
    22081 taaccgagat ggattattcg attacactag aacaaatgag agaaatggct aattatggaa
    22141 ttgaatttgg ttcgcatact gtgacccacc cgagattgga tacgatcggg ttggcggagg
    22201 ctgaagagga aatttatcaa tcaaagagga tgttggagca atattataac cgacaggtga
    22261 aaactttttg ttatccttat ggcgcattta acgacgatgt tatttcatta gttgccaaaa
    22321 gtggttacca atgtgccgtc gttactccga ctcgtccagg gattaaagaa actcgattta
    22381 ccttgattcg ggttggtgtc tataattcta actcactctt gaaattcaaa ttcaaatgtt
    22441 caaacctatt tacaaacatt agaaaagcaa gattatggtt tctgttgaag catttatcaa
    22501 tttatttaaa aaagaaaagc acaattaaaa cctcctttgt ttggtgaaat ataataaccg
    22561 ggttggagaa taaaaattat ttgctgataa tacaaaaatt acttgacctc atcaaaaaat
    22621 acttgtagat ttaatcacga tgagtaaaga agaggaaaaa gactaaatca acttggtacc
    22681 ggcctcgatt ctttaaataa ccctaaaatt tatcaactta gaaaaactgt aaatcttatg
    22741 ttttgagtag aaatttaata ttcatttagt aatcttggct ggaggaatgt agtggctttg
    22801 gcatctttat tagcagtctt tttgactacc tatatatgcg cgttaggatt gacttatcta
    22861 gcacgcaaga tagctctccg ttatcatatt ggcgatatgc ccagtgaacg gaaagttcat
    22921 aaagtgttta cgccttattt aggtggtgcg gcaattctcc tgtcaatcat aattggtatc
    22981 gtattatcaa tctatttatt acctatggtc gctgaagagg tcccgcggcg atattatttc
    23041 ctaggtataa ccggtgtgct aatcgccctg gtaggattgt acgacgatat taaaaagtta
    23101 cgtttttcta ccaaatttta tttccaaatt ctcgcggcta tcatcgtggt tttaggtggc
    23161 ttaagatttg aatctttata tattccgatt attggtgagg ttgggttagg ttggttttcg
    23221 ataatcttta cgatattatg gattgtattt ataaccaatg cgatgaattt gctggatgga
    23281 ttggatggac tagcggctgg agtttctacg attatttttc tggcttttgc attagtagca
    23341 ttacattccg gtcgaacatt ggtagcctta gtttgtctat tatttattgc agctaactta
    23401 ggatttttaa aattcaattc tcatccggct tcaatcttta tgggtgatac tggttcgctt
    23461 tttattggtt tttcaacggc agttatttca ttagaagtag gccgaattgg tcacactaat
    23521 tgtctggata ttattattcc gcttacgatt ttagctgtac caatattgga tactacgatt
    23581 tcctttttcc gacgaatcaa taagcatatg aacccattta tggcggataa ggaacatatt
    23641 catcatcgtt taatgtcaat tggattttcg cataaaaatg cggtcaaggt aatgtatttt
    23701 ttaagtatta ttgccggatt tatcggctgt tctttcattt accttgataa tcgggcgatc
    23761 gtgacaattt tattcgccgg tgcaattttt gtaggtttag tgatacgccg actgggttat
    23821 tttgagatcg agaaaaacct aatcgttgtg cccaatggta ccaataataa cagcaagacc
    23881 aatggattta gcaatggaca ttcagagcca agatttatcc cgtttgatac gtcgcagttt
    23941 attcaatcct ttatcttttt cttgagtgat gtcttattta tcgttggttc gtttttagcg
    24001 gtttattatt ggcagattgt tcccaatctt ttaccggagc aattgttgac tccctataat
    24061 ttaattttat ggatcgcctg gtcagttatc tattggactt cgttattggg cttgaataat
    24121 ctttaccgaa ttgaatggga tacttcgcga gttgatgaaa tatttactgt ttttaaaata
    24181 atatttttcg gcacgattgt attattcttt ctgagttttg aattcaaaat tccctttctt
    24241 ccttcgaggc gaatattagt cacttatggt atttttctgg ttactggtat tagtgttggt
    24301 cgtttagtat taattactat tttgaaaaaa tacaatttgt tagaatttaa aaaacgtccg
    24361 acattaatta tcggtgcttc acgtcgtgca ctcaatgtag ttcggaaaat aaattctgtt
    24421 cctgagttaa aatttaacat agttggaatg gttgatgatc acgctaatgg aaatatcggg
    24481 aaaataattg aaggggtacc gattattggt agttttgaaa acattcccga gattattaaa
    24541 aaaagaaaaa ttaaagaagt cattattgcc cttgatgaca ataatatgaa tcaggttatt
    24601 gatatgattg atctgttttc aaggtacaac gtttcgatca aattactgcc tgatttttac
    24661 aatctactca ccggttttcg aaccagtcac atttatggag tctcattggt aaagtttttc
    24721 ggctctaata tgaaaacctg ggaatgggta ttgaaacggc tcattgatat cattatttcg
    24781 ttagtagttt taatcggatt tctacctatc tggctaatca taagtttgat aattaaactc
    24841 gactctccgg gaccggtatt ttataaacaa aaaagaattg gcaaaaatcg tcgcgaattt
    24901 gaagtaatta aattccgttc gatggttcag ggtgccgagc aggaaaccgg accgaaatgg
    24961 gctgaaaaaa atgatccgcg agtcacccgt ttcggtaaat ttttgcgaaa aactgggctc
    25021 gatgagattc cgcaattttt caatgtcctg atgggtgata tgagcattgt gggaccacgt
    25081 cccgagcggg attactttgt taatgattta gaacgttcca ttcaatttta ttcgcgacgc
    25141 ttaatcatca aacctggcat caccggctgg gcacagatta aatataaata cgatgaaacc
    25201 attgatgatg tacgcgaaaa attgcgctat gatttatatt acatcgaaaa tatgtcagtt
    25261 attttagact taaaaattat cattcagact cttttagttg gtctccgtcg taaacaccgc
    25321 accagctacg tctaaaaatt agcaactgga aataacgatt agattataat atactctatt
    25381 atcattccaa aattttttct attttataag gttgaaatgg agaatgaatg ttagacatta
    25441 aacaaattag aaacgaaccg gaaaaatttc ggcgaggatt ggaagccaaa aatgccggtg
    25501 ataaattgga tgagctgtta aaatgggatg gcgaacgccg aaaatgtatt aaccaagctg
    25561 atgaattgaa ggctattcgc aatcgggtca gtgacgagat tggccaattg aaaaaaaaag
    25621 ggttaccagt cgcagaaaag atcctggaaa tgcgctcggt aggcgatcaa atcaataacc
    25681 ttgaccataa attgaaagaa attgatgaaa agataaatag tatcctaatt actttaccta
    25741 atctaccgca cacaagtgtt aaaatcggaa aatcagccgc tgataatgtt actataaaag
    25801 aatggggaca caaaccggag tttgattttc ccttaaaaga tcatttagat ttaggagaag
    25861 cactgggaat tcttgatatg aaaagagccg ccaaaatttc aggctcggga tttcctatgt
    25921 atatcggaga tggtgctact ctcgagcgcg ccttagtcaa ttacatgctg gattttcatc
    25981 ggaatcatca cggatatcag gaagtctttc cgccatttat ggttaaccgt gattcagcct
    26041 tcggtaccgg tcagttacct aaactcgaag aagatatgta tcacattgaa attgaggacc
    26101 tttttctaat cccgacagca gaggtaccgg taacgaatat tcatcgtgat gaaatacttc
    26161 cggaaaaccg actgccgatt aaatatacag cttactctgc ctgttttcga cgggaagccg
    26221 gttcgtatgg caaagatacc cgcggtttat cacgcgtgca tcaatttaat aaagtggagt
    26281 tggtttggtt caccacgcct gagaactcct accaagtgca cgaacagata ctgcgggacg
    26341 ccgaagatat tttacaggct ttgaatctcc attatagagt cgtggaactt tgttcggctg
    26401 atttgagttt tgcggcggct aaatgttacg acatcgaagt ttgggcaccg gcaagcggaa
    26461 aatatttcga ggtttcgagt gtcagtaatt ttgaggactt tcagactcgc cgggcaaata
    26521 tccgttaccg ccgaaccagc gataataagg taaatttcgt gcatacttta aatggttctg
    26581 gtgtagccac tccccgactt atgattgcat tactcgaaac ttgccaaacg ccggaaggca
    26641 caatcataat tccgcaggtt ttacagaaat atactggatt taatcagata aggaccaaaa
    26701 aatgacaggt gaaaaaaaat atagaaaact tatttgcgaa atcggcaaga aaatctatgc
    26761 caatggttat gttgcggcta atgacggcaa tctcagcatg cgtttgaatg cggaaatggt
    26821 tttaattaca ccgactaatg tatccaaagg tgcggtgaaa ccttctcaaa tagctaaaat
    26881 agatttatct ggaaatccat tagataaaaa tcgtccgtcg tcggagtcac tcctgcattt
    26941 ggcgatttat aaacgatgtg attgggtcaa ggcagtcatt cattcgcatc cgccttatgc
    27001 cactgcttgg gctgtaattg gtctgggatt gacggatcca atcttgcctg aaactatcgt
    27061 atcaatcggg caaatcccgt taatcccgta taaaccgcct tcaactcaag aattggcaga
    27121 tatagtagcc gatgcggctc agaaaaacga tgtgctatta ctccaaaatc atggattagt
    27181 agcagtcggc aaggatttat tggatgccta ttacaaaacc gaacgcgccg agcatgtctt
    27241 tcgaattgtg gcgttggcta aacaaatagg agaaattaga accctgagca aagcagaagt
    27301 tgcagaattg tatcgtttat ttaacgtatc cgaaaaacta caagtaactt atcgatagtt
    27361 cttatatgat taggaatttc tttgctgtaa tctgggtagt tttagttacg attgtatttg
    27421 gtagcctgag tatggctttg ccaggaagcc ataatacaat gatgcgcaaa tggtcgcaga
    27481 tgattctcca atttcttggg gtcaaattac aggttcatgg ccttgaaaat atagtaaatc
    27541 gaggttcaaa aatcgtaatt agcaaccatg aaagtgctct ggatattccg atcttgatgg
    27601 cggctttacc agttcctatt cgttttatgg caaaaaagga attatttcag attcctcttt
    27661 ttggatggac acttaatctg gcgcgtcata taccagttga ccgtaaaaga cctcgcaaat
    27721 catatacggt aattaaccgt gttgctaaag aattaatccg gaacgggctc agtattgctg
    27781 tcagtccaga gggcactcgc agttacgatg gtaaaattgc accttttaaa aagggcgctt
    27841 tccgattagc tcatatacat aacctcccga ttcttccggt attgattctc ggagctcggc
    27901 actgcgttcc gaataagagc ctttcgatca ttcccggaat agtcgaagtg catattggga
    27961 aagaagtcca tttgagtgat tatccgacaa tgaatgattg cgtggctgat ttacgtaacc
    28021 aaatgattgt ccaaaaggaa caatacgaag aaaaaagaaa gcaaggtagc cttgattaac
    28081 tgcatcccaa accggattgc cgaaccagaa atatccatta ccaaaaagga aaaatccctt
    28141 tacaaagcct ggttgaatca ggaattcaat ctcgcggatt tgcaaacggt tgaaggctac
    28201 tcgctcagta tattatcacc cgggaatcga aacgaaactg aaggtccgga ttttcacgat
    28261 gctttgatta tgttaaatga aaagttcgag cgaggtgacg ttgaattaca tctcgaatcg
    28321 aatgactggt acagccatgg acacgaccgg gaccctcatt acaataatgt gattttacac
    28381 gtggttttaa actcaggtaa attactaccg ataaaatctt gtgacaaccg tattattccc
    28441 acattgatca tgacgccatt tgcgatctca gcacctgatg aatatgcttg cttaagctgg
    28501 aaacatctat ctaacgacga tctcaaccaa gttctaaaaa cattcgccga aattcgcttc
    28561 aaacgtaaag cccaaatatg gcgctcactg attttaacaa aaggcgctga gcagacatta
    28621 tatgaaggac tggctgatgt gctgggatac agtcggaatc ggaaaatgtt tggaatacta
    28681 agccaaaaag tgacccttga attgatacgg aaaattttac gggatactga tcccgaacag
    28741 cgcattatag tattggaaag tcttctattt ggttcggctg gatttctgac caaacctgaa
    28801 ttaaaagtat tcatcagaga tacaaaatat tcagaaggtt tatataaaat ctgggtgaaa
    28861 atatcgcaaa aatacgaact ggaagaattg gaatcagcgg actggcattt cgccggatcg
    28921 cggccgccga attttccgac tttgcggatt gcggcactag cccagataat taataaattt
    28981 tatcctaact atctagctga aacttggtta agagtaatca aaaatgccaa aatttttact
    29041 gatattcgac gctatgcaag tgaaatgttt caacaaccgg acggactctg gcgcaatcat
    29101 ccattattga aacaacagcc gggaaaaatg ctgatcggcg ttcagcgctt taacgatttg
    29161 ttgagtaatc tgctattgcc attcagctgg gcgatagcgt ccctcgacaa tcagtatgaa
    29221 ttgattgaaa aaaccactac tttcgcggag caggttgagt gccttgaagt gccgggatca
    29281 gttaaggttg ctctgaaccg attgccactg gatactatca ctttgccccg aaattattta
    29341 attcagggaa taattgagtt ttcacgtcgg tattgtaaca tgaatatttg taaattatgt
    29401 ccgttagaaa aatatgccca aaaatgataa aataatcacg cgtgaacaag ccggccgaat
    29461 cgttagtcaa ctcaaaagag aaggccgaaa ggttgccttc accaacggct gctttgatct
    29521 tcttcacatt ggccatcttg aactattgga gcatgctagg aaattaggtg atattctgat
    29581 agtcggttta aattcagatg attcggtacg acgcttaaaa ggtcctcagc gcccaattaa
    29641 cactcaatta gatcgtgctc ggattcttgc cgctctggaa gtggtagatt atgtagtaat
    29701 atttaatgaa gatacccctt tagagttaat tcaaaccatt aagcctgatt ttctgatcaa
    29761 aggtggtgat tatagcccgg atactattgt tggtagtgca ttcgtcgaat cctatggcgg
    29821 aaaggtaatt gtatttcccc tggttcatgg caaatcaact tccgacctga taaataccat
    29881 atttcaactt taatcaataa atgctcttga cattaagttt tgtttgaaat aatttcccac
    29941 ccaaaaatat atagaatgga gtgaaatgaa taatagaaag aaattgagat taatcttatt
    30001 attttggatg acgataacaa ttagtgtagc attatctgct aataattcct atgaaaaaat
    30061 aaataacaac aatggagaag agaaagaact atcagataat tctgggaatg atatttttga
    30121 acttattttc aatgaagcta agttattcta tgtagatgct ttagtatctt cttattataa
    30181 tgacacagcc gccgtaaaat actgttttga tcgcatattt gaaattatcg ccgaaatcag
    30241 tgaacttgat agcctgactc tggttgaaca agatgacttc aaccgtttta atgagatggt
    30301 atcaaatgac tatcaaaccc atttttcgta cttaatagaa gaaactgact cagtgatgat
    30361 agtttcggta ggagaggaat tatttgtaac accattagat acggtcgata taggcaatga
    30421 taccttaatc gtagtcgaag accgaccagg acatattcca atcgttcgat ccaaaaaaat
    30481 cgacaaactt ataatatatt attccggtca gcaagccccc gtaattcaaa gatatcttga
    30541 agcattcaat aaatataaag atcattatct cccgattcta aaaaaatata acgtaccgga
    30601 agaaattatt tatttaccaa ttttagaaag cggttataat cctaatgcct attcttatgc
    30661 tcatgccgcc ggaatatggc aatttatcgc tggtactggc gccaaatatg gtctaaagcg
    30721 taactggtat gtagacgagc gtcgtgatcc gattaagtct gcccatgcgg cagctaaata
    30781 tctcagaaag ctttatgaag aatttgacga ctggtattta gctttagcgg cttataattg
    30841 cggtgaaggt aatgtctggc gtgctatcag acgcgaaggc acccgtgact acttcaaatt
    30901 acgctcgtta ccctcccaaa ctcgcaatta cgtaccatct tttatggcta tcacaatcat
    30961 tgccaaaaat ccggaaaagt atggacttac cacacccacc acacccactt gggaatggga
    31021 agaagtgata atagatcgtt gctatgaact cgatgtcatt gccaaagctt gtgaaactac
    31081 acccgacatc ctgcggcaat ataatcccga actgcgacgc tggatgaccc cagctaatga
    31141 taatcaatat gtactgcggg tacccaaagg taaagccgag ggtttaacag aaaaattggt
    31201 atcactacca gaaccaaagg acgtaaaacc cgaggtggtt tatcataaag ttcggaaagg
    31261 tgaaaacttg agttcaattg ctcgtaaata tggcacctcg gtttcggctt tagtttctgc
    31321 caatcacctt cgtaatcgca accaattacg tgtgggtcaa gtgctcctga taccgactgg
    31381 ctcctattat tcggcacctg aaaaaaaatc gtcccaaact aatgtagtaa cccatgtcgt
    31441 ccaacgaggt gaaacattaa gtgaaattgc tgaaaagtat aataccagtg taagtaaaat
    31501 aagatcaatg aataatattc ataatagcca tatacaggtt ggtcaaaaac tcaaggtacc
    31561 tactaatcta gccagccaga tagaaccttc cgacaatccc aagggcagta ctaaagtaat
    31621 ccacgtcgtt aaaaaaggag atactttaac ttcaatcgcc aataaataca atgttagtct
    31681 tacaaaattg aaatcgtgga acgatctgga tggtcgcaaa ccaatttatc ccggtcagcg
    31741 gatcgtaatt taccagtcaa ttaaaagcta agtcaaacgg ttttcccttt ctgaaagtct
    31801 gaaatgttgt gtcatataaa tcgtgctcga tcaaaatcct ctactattat gacaaaagga
    31861 taagcctaat tatagaaaaa taatggcttg cctttattat gaattctata aacatctctt
    31921 tgatgtctaa tgttggtcat ttaattttga ctatggtatc ttctgaaatc cttaataccg
    31981 aaagacaccg cgacggggta aaatatttct gcaagttatt ccgtatcgaa tcctcactac
    32041 cttccataaa ccacgtcacc agtattcttc gcaaattttc acttcttccc tatgaaaata
    32101 tcagcaaaat ccttagtctg aaccaaaatt gggacgaaaa tcatttccgc tggcctcagg
    32161 aaatcatctg cgactatgaa aaatataaat taggcggtac ctgtttttct cttacttttt
    32221 ttctaaaggc aattctcgat tattttgggt atcaaaccac aatattaatg gccgatatga
    32281 aagccggacg aaatactcac tgcgcactct tgcttgaatt caaaaaccgc aaatatctac
    32341 ttgatcccgg ctatttactc cacttccctt taccattaaa ctcggccgtc atttctgaga
    32401 aagtccaatt ggaatatgat ccattttgtg agaggtattc actctcgaca ttagaaaatg
    32461 gcaagaaaaa atggcggtat tcatttcagg aaagaccgac ttctatcgag gattttagtc
    32521 agtactggga agattccttt cattggatga ctatgcacgg aatctgcctg tccaagcgcg
    32581 acgaaaataa tttcatttac ctgcataatc attatttaaa attggaaaac agatcccagc
    32641 attttaaagg aaatttgaat acggaaattg cagaactggc tcagcgttgg tttaatattc
    32701 caccggaaat tgttcggcaa gccgaaaaag ctttgcgtga aaatttatac caagataaaa
    32761 tattaggtta tcaaatacca aaatggttta aatagcaatt gctaaaacac ttgaaaaaat
    32821 aaaatatatt aaaaatcttg cttaataacc acttagaaaa cagattgtgg ttaaaatatc
    32881 agactagact aactcttaat tttttagttt gagaaaaata caggggtatt tttatcccaa
    32941 ataacctctt aaatgtaaag ccctactatt atgccgtaat tttcgtaagg ctttttcttt
    33001 aatctggcga acccgttctc gcgttaaatg taaacgttcg gcaatatctt ccagatttaa
    33061 cggtagatcg ctgtcaatac caaaataaag acgtaagatg taggcttccc ttttgtctaa
    33121 agaatttaat gctcgctcga tctctaagtg aaatgactcc tggtttagag taagtccggg
    33181 atcattatct tcctggctag gtagaatgtc ttgtaacgta ccggtttgat cgttgccgac
    33241 tggagcatca atactgatag tattttcagt taattggcgg gcagaatcta tgtctatctt
    33301 ttcattttgc aacaacactt caatttcatc gttggtcggc tctcgttcat aatccctttc
    33361 aaattctgaa gtaattttgg taatcttgtt aagactaccg actacgttaa gtggtaagcg
    33421 aaccagacgt gaattttctg agatagcctg caagatagtt tgccgtatcc accagacagc
    33481 gtaggagatg aatttaaatc cacgagtttc atcaaaacga cgcgccgctt tgattaaacc
    33541 gatattacct tcattaatca aatcagcgag agaaagacca taaccctggt attttttagc
    33601 aactgatact acaaaccgaa gattagcttt tagaagccga tcaagagcct gggtatcacc
    33661 ggctttgact tttctggcta gttcaatttc ctcatccggt gtgagtgtct tttcagtccg
    33721 gatttcatca aaataacggc gtaaaacctc gttggagatg aatggtgatt ttctagatga
    33781 agatgaataa ttagtcatgg ccatttccgg ccagggtaat aatggttaag tttctgtctc
    33841 acttttagat tgatccggct catccggttt agcagtattt tctttagaac tttcctgaat
    33901 cttgtaatac tgggcttctt tatcttttaa ctttttctcg agttctttaa tgttattaat
    33961 caaactggtt accttttcat ttttaacgag attgccttta gtattttgat tcagcaatcc
    34021 ataaactact cgacctaatt cggtgtaatt cttatcaata tccctcttga tttgaaaaat
    34081 atccagttta attttgccca gctgggtcat ttcctcggct ttctcggcgg cttctcgaga
    34141 taattcaccg gctttttcag cggctttaga tgcaaatcct ccgacacttt ttttaatatc
    34201 atcccaaata ccagtcatat tgtcctccta attttttttc ttactattta aaaaatccac
    34261 ctaaaattat ttataataaa acataatgtc cacaaaaaat aagagcctcc tacaattagg
    34321 aggctctttt atggacaatg tctttaattt ttaatacatg tcgcccattc cacctggcgg
    34381 cattgccggt gcggctggtt ctttttccgg cacttcggtt attacagctt cagtagtcaa
    34441 catcaatccc gcaatacttg cggcattttg gagagcgatg cgggttactt tagttggatc
    34501 tataattccg gctttataca tatgaacata ttcttcttta tatgcatcaa atccgaaatc
    34561 atctttccct tctttaattt tctgggcaac gatagatggt tcatgaccgg catttttggc
    34621 tatctggcgc aaaggctctt cgagagcacg tttgataatt tttacgccaa ccatttgatc
    34681 gccagtaact ttaattttat ccagagcagg aatgcatctg attaaagcaa cgccaccacc
    34741 agggactata ccttcttcca ctgcggctcg agttgcatgc agggcatctt ctaccaaagc
    34801 cttgagttcc ttcatttcaa cttccgtcgc ggcaccgact ttcaggacag ctactccgcc
    34861 cgagagttta gctaagcgtt cctgaagttt ttctttatca taatccgatg tactggcttc
    34921 aatttgagct ttaatttgtt tgattcggcc tttgatatcc tcgggtttac cggcaccttc
    34981 gacaatcgta gtattgtctt tatctaccac gattctcttc gcatttccca gatgtgagat
    35041 agtagcattt tccaatttgt aaccggcttc ttcagatata actttaccac ccgttaatac
    35101 tgcaatatct tctaacattt ctttacgacg atcgccaaat cccggggctt tcaccgcggc
    35161 aacttttaaa gtgcctctta gcttattgac aattaaagtg gctaaagctt cgccttctac
    35221 atcctccgca attatcagta aggaacgccc catctgcgac actttttcca aaatcggcag
    35281 caattccttc atactgctaa tttttttgtc ataaatcaac aaataaggat cttctaaaac
    35341 tgcttccata gcctcagcat tcgttacaaa ataggctgaa atatatccgc gatcaaattg
    35401 cataccttcg accaagtcca gagttgtcag agtacttttg gcttcttcaa cggtaataac
    35461 gccttcctta ccgacttttt ccattgcctc ggcaattaat actccaatcg gtatttcgac
    35521 tatatcctct ttacctgatt catcttttat catccggcgg actttccggt tattagctga
    35581 gatagttcct acctgagcta tttcttcttt actgtttgtc acagctttgc tcaattcttt
    35641 taggctttcg actaccgtat ttacagccgt gtcaatacca cgtttcaact ccatcggatc
    35701 ggcacccgcg gtaacgttct tgatgccttc agcaataatt gactgggcta atacagtagc
    35761 tgtggtggta ccgtcaccag caacatctga agttttagat gcgacttctt ttaccatttg
    35821 cgcgccgatg ttttcaagtt tatcttccag ttcgacctct ttggctacgg taacaccgtc
    35881 tttggtaatc gttggagaac caaatttctt ttcgataacc acattacgtc cttttggacc
    35941 aagggtaact ttcacaacat tagccagttt atcaacgccc tcttttagag cagttctggc
    36001 ttgtatttca taagtgatgt cttttgccat atttgactcc ttttgttagc attaaagtat
    36061 tgcaaaaatg tcgctttcgc gcattatcaa atgttcttca ccatcaatca ttacttctgt
    36121 accagaatat tttccataca agacatgatc gccgactttt acttccagcg gaattttttt
    36181 gccatcatca gttgtttttc cagggccgac agctattact gtgccttcct gtggtttttc
    36241 tttggcagta tcaggcaagt atatgccacc tggagttttc tcattagaaa ctaggggctt
    36301 aactaagacg cgatctgcta atggtttgat tgccatagaa tttctccttt caattaattt
    36361 attaattatt gattaaccaa ttgtttttag ccattattct ccttcaagca ttcaaagagc
    36421 gcatttaaat ttacaaatga cgtgccagtt ttcggctcgg acagaaagtc agagaaaaat
    36481 atttcagctg actgtaacca tagatagatt atttctagta aaatcctttt aatgtaaatt
    36541 ttgttttaat tctttcgtat tttcctatga cgattatgat cctgatatga taagttcaaa
    36601 tttccttttt attctggtgg gatttgtggc tggtgttctt tccggcttgt ttggtatagg
    36661 aggtggtatt gtaattgttc ccattatgat ttggttctgt catttttctc aattagccgc
    36721 aaacagcatt tcacttacgg ctttacttct accggttggg attctggccg ttttggctta
    36781 ccataaatcc aagttggtta aaatacgcca ggctttattt ataagtttgg gaatttttat
    36841 cggcgtggtt ggcggcgcta ttttagctct tgagattccc gctcatttcc tgaaacgact
    36901 ctatggcatt ttccttttat atattgcttg gacttatatt gacattccat cattatttgc
    36961 aaatggagct aaccaagaga aaactaacca gcaatttgaa aatttaaatt tggatcgacc
    37021 agtttgttat atagtgctgg gatttgcggc gggaataatg tccggcttat ttggcatcgg
    37081 tggcggtatt ataatcgtac cggccttgat ctcaattttt aaatatccat ataaaaaagc
    37141 tgtcggtaca tcattgatgg ctttactttt accagtagga ctgccagggg tgttgattta
    37201 taatagcgcc ggacaattac aaatcggtcc agcgctattt attgctctcg gattattatt
    37261 tggtgcgtta gttggagctt tagttgcaat cgagttgcct actaaactaa ttaagagaat
    37321 ctacggggga tttttattgt tagcaggctt taattttatt ttttaataaa atcaagacga
    37381 taccgctaaa gcggcttcta atcacggttt aacgacggtt actttcctcg attttagaag
    37441 ctttgccccg caaacctctc aggtaatata acttagcccg acgaactttg ccgacgctga
    37501 ttctttttat tttcgatatt ttcggagagt ggagtggaaa aatcctttct actcctattc
    37561 cattggagat ttttctgacg gtgaaagtct cactaattcc actgccgtga cgagcaatga
    37621 cgactccctg gaaagtctgc agacgttctt tatcgccttc aataatcttg acttcaaccg
    37681 cgacggtatc acctggtttg aattcaggaa tatcagtcct aaactgatcc gcaactgcgg
    37741 cggttaattt atccattatt tttatcctcc atattcatat ttttaattaa atcaattctt
    37801 ctttggcgag ttctaagttt gcgttgttcc tggcgccagg cgtttatttt ggcgtggtta
    37861 cccgtggtca agacctctgg aacacttttc cgtcggtaaa cctgtggatg ggtatatatt
    37921 gggccttcca gtagattatc cgtaaaggaa tcggtctcag ccgactcata ggaatggagt
    37981 acccccgcta tgtgtcggat aactgcatca ataataatga gcgccgggag ttcaccgccg
    38041 gtcaacacat aatcaccgat actgatttca tcagtcacca ggttagcgat tactctttcg
    38101 tcaacacctt tgtagtggcc gcaaaaaaaa gtcaagtctg actcctgagc taattcgagt
    38161 gccaaatgat ggttaaacaa tcttccctgt ggcgttggaa acacgacgcg cggtcgttta
    38221 tgcccagtca gtttgacaat tttatcataa gcacgaaaaa acggctccgg ctttaagacc
    38281 attcccgcac cgcctccgta gggtttgtcg tcaatctgtt tatgtttatc gcgggtgaaa
    38341 tcccgtaaat tccagacatt aaattcaacc ttaccatttt cgacggcttt gcggaacatg
    38401 ctttcagcta aacaggctgt aatcattccc gggaatgcgg tgattatgta aattttcatt
    38461 tcggaaataa accttcgata tctttaaaaa taacaatttg ctgttcaggg tcaatctgtt
    38521 caattaaatc ggttactgcc ggaacaagga gggtcgcgtc ttccgattga atgacaaata
    38581 atgcctgcgg attggtttcg tccaatgaac taataaaacc gatatctttg gctgaattga
    38641 ttactctaac cctgaatccg ataagctcaa cgagctcttt tttcaattca tattctcgtg
    38701 gaacgaaaac ctttagtcct ctaagataat ccgcttcctg ccgggaatta atatctcgca
    38761 atttcaaata aactttttta gtacttattc tcagataatc aaccttccat gggcgagcta
    38821 cctgagcact ttcaccgagc caaagtactt ttggctcatc tacttttata tttttcagag
    38881 agccgcgatt taaaaggact gccccgttca gaccaaagct ttttaaaatg gatccgaccg
    38941 gtaacagtac caaatgatcc attttaacgc ttattcaatg atttctaaaa ttgccctttt
    39001 ctgaccttgt ttggcggcgg cggcagtcaa aagtgttctt atcgcgcgag cggtgcgccc
    39061 ttttttcccg atcacttttc caagatcacc ttccttgact gaaagttcca gaattacggt
    39121 tttttcacta tcaacctgca ccacttttac atcttccgga tggtcgacta attgttttac
    39181 gatgaattca acaaactctt tcatagttgt cactcctctt ttctagtttc agacaatttc
    39241 aatactacta ctcaaatcta ctttaaaatt tagtaataaa tagtcaaaat atctttaaga
    39301 cttaaaattt cacatttctg tctggacatc agtcttagat tcggttactt cgctggaagt
    39361 ttcactagtc tcttcggata attctatttc gggaattact ttttcttcag cggatgcaat
    39421 cgcttttgct tttttagcgg cggctgattt aggttttcca ggtgtttctg ctttttcagc
    39481 cggtttggtt tcaggcgtta attcttcttc gacattctcg acgactggga cagattcggg
    39541 ttctgggacc tcgacggctt gtgcttcttt aacttcttca gcgatttcag cgcttttatt
    39601 atgagctaac tgccattttt ggatttcgat atttcttacc tgttcgctga tattattgcg
    39661 aatcaaatgc cactccattg caataccttc tttctgtaat agattgaaaa cagtatctga
    39721 aggaacagca cctttttcta accaactaaa aaccttttcc ttatctattt taacggttgc
    39781 cggtttggtc agcggatcgt aataacctaa tttgtcgagg tatctaccat cccggcgaca
    39841 tttactgtcc attgccacga tccagtaaaa cggtttcttc tttctaccca ttcgttgtaa
    39901 tctaattttt actgccacta agagcctcct tttatttaat tacctattcc aaaagcagat
    39961 aaattcttgg gtagtttcat ccgacccatt tgtttcatta acttagttat ctgccagtat
    40021 tgattaagta gacggttgac atcctgcggc tgagtaccac tccccagagc aatccgtcgt
    40081 cgccgactac cattaattat attgggttta agccgttcgg ttcgagtcat tgattgaata
    40141 atcgcttcgg ctttaatcag ttctttttca tctacttttt gatttccgag tcgcatacgg
    40201 gcaaagccag gcaagtgttc cattacgcta tcaagcggtc caagtttttt taactgtcgt
    40261 agctgagtca aataatcttc gagggtaaat tgttcttttt ttaatttatt ttctaatttc
    40321 aatgccgatt caaggtcaac gctcgcttgc actttttcga ctaatgagac aacgtcaccc
    40381 atcccgagaa ttcgcccggc tacccgctcc ggatggaaaa tttccagatc gccgatcttt
    40441 tcaccgacac tcatatatat aatcggtttc ccggtagaag aaacgatcgt caaagcagct
    40501 ccaccgcgcg tatcgctatc cattttggtt aatatgattc cgtcaacacc aatttgttcg
    40561 ttgaaagaag tagcggtgtt gactgcgtcc tgcccggtca ttccgtcaac aaccagaagg
    40621 atattatgcg gtttgatggt atttttgata gtttttaatt ccgccatcat tttttcatct
    40681 atgtgcaaac gcccggcggt atcaattatt agcgtatcgc gatgttgctg tcgtgcaaaa
    40741 gtaattgcat cttggattcg cccgactaca tcggggttct cgcgaaaaca gggaactttt
    40801 atctgtcgag caagaatctc taactgctct tcagcggcgg gacgataaac gtccacaggc
    40861 accaataccg gactatgttg cttctgaagt aattgattag ctaattttgc ggcgcacgtt
    40921 gttttcccgc taccttgcaa accgaccagc ataatgatag tcggcggaat agaagcctcc
    40981 ttcaaattgc ggtttttatc accaagcaga tgaaccatct catcatagat gattttcacg
    41041 attaattgtc cgggagacag gcttttggca accgttaccc cgactgattt ctcctgtacc
    41101 gactgaacga attcttttgc aaccttgtaa ttgacatcgg cttcaagtaa aacccgccta
    41161 atctgacgca ttgcatccgc gatattattc tcagttatcc gcccttctcc acgaagggat
    41221 tttatgatcg caccaaaacc gttttgtaat tgttcaaaca ttgtttaatt caaattcatt
    41281 aaagcgggta aatttaattt ttttaacttc tttttgcaag atattatcct cacaggatta
    41341 tatagtatca agaaatggta tcaaccgttt catcactaaa gaatataagg atttatttga
    41401 aaataaagga agattgagtt accttttgct tgtgaatgct aattaaaact aaaggattat
    41461 catgaataga agacatctaa tgatcgtttt gacgttccca atattcttaa tgtataattg
    41521 ctccgaaaca caacctgagt ccagcaatcc ctaccgaata aattcgggac tcaatcctgg
    41581 aataaatatt ggtaatgctc tcgaagctcc gaacgaaggc gaatggggcg tggtgattaa
    41641 agatgaatat ttcccattga taaaatcggc cggattcaat tccgtccgtt taccgattcg
    41701 ctggtcagcc catgctgata ttaatccacc ttatacgatc tcggacgatt ttatgaaccg
    41761 agtgcgtcac attgttgaca ctgctcttga gcacaaatta tatgtagtta tcaatgttca
    41821 acattttcag gaaatttaca ctgaacccat agaaaataaa ccgcgcttgc tggctttctg
    41881 gcatcaaatc ggcttagcat tcaaggacta cccgcaaact ttacttttcg agccactcaa
    41941 cgaacctcat ttcaatctca cccccgaatt gtggaatgaa tggatacctg aattaattga
    42001 tacgattcgg gtcacaaatc ccaatcgcac tttggtaatc ggaacagccg actggggtgg
    42061 aatctggaaa atgaaagaat taaagttacc cactactgaa cgaaatctaa tcctcgcaat
    42121 ccactactat gaaccattcc ggttcactca ccaaggcgcc gagtgggaaa aagaaagtaa
    42181 tgcctggctg gggcgcactt ggaccaaaac caccgatcaa atgcaagaac tcaaccagca
    42241 tttcgatcta attaaaaaat ttgctgactc gttaggagta ccggtttata tcggagaata
    42301 tggctgttat aatcgtgctc ccgaagaatc tcgattaatt tggacacgaa cagtctccga
    42361 acaatgtgaa aaatatggat ttagtcgtgc ctactgggaa ttctgttcca gtttcggtat
    42421 ttatgatccg atcagtcaac aatggcggga gccgcttcgt tcagcagtat tattggcgag
    42481 gtgaaaatta ccaaaatcat tgatcatcaa ttccgaaaaa ctttcccctt gaatcactct
    42541 actagattaa aattgtcaga taagcggtaa ttctcattag aaattgaagc tatgacggaa
    42601 tatttcataa ccattatgat ataaccggca ggtgattaat cctggtgaaa agtaatggaa
    42661 cttaacttcg tcttaatact ttaattttgg tcatagctaa aatcagacgt aattgcatat
    42721 cagaaatcac caatgtaaat tccgataaat ttgattttta tgataattaa caaacgattc
    42781 tgcgctaaaa taatattcac ttcattttgt ctattcagtg ctatgcttgt tcggctcgag
    42841 gctgtcgaat tgacaatttt aagcgaaact acttcagagc tgattcttcg ggtcgaaatg
    42901 gatgccgact tagaatggcg ccaaatagaa aattacccga ataaagtcag cgccttgact
    42961 ttaggagacg cctaccaatc ccatttgagt caaaccctgg aaattcctta ctatcaatgg
    43021 ctaattgcac ttccgactga ggataaacct tcagtatcca taaccaaatc tgtttttgag
    43081 tcagtcaaac tcttaaaaaa cctgactacg gacgagttaa cggctctcga taaatttccc
    43141 ttggttgcga tcgccgagac cggtttttta agagatgttc ccttcggttc gcttgtaata
    43201 tatcccctgc gttcagcaaa taattccaat gaattacgtt tattgcgcga gattgaaatt
    43261 acaatacagt acacagcagt aagaaaaacg actatcaatc agaccacggt agaaaagggc
    43321 gattatttaa atgcttttgt gaatggatca tcagccacac aatggcaaca atctcgccca
    43381 cttagtctac ggaaacctca gagccaacct gccggaaagt ggtttcgcct aacggtaaaa
    43441 aaggatggcg tttacactct agccctcgac gacctaaaaa gcgccggatt gactgagaca
    43501 agtatagata cgggacgtat ttatctctac tctaattcca ccggcgggcg tcctctggat
    43561 gcaacaattg gcgccgctgt tccatcgaac ttaatcgaaa atacacgctg gataaaaggt
    43621 aataacgacc aagattttac taacgctgat acgattcttt tttacggtcg ggctacgagt
    43681 ggcgttgacg ttgattctaa aaataatctc accttcgtgc gaaatcctta ctcgctaata
    43741 aattattatt ggttattcat cgccgatgag cccggctatc ctaaggctat gccgactata
    43801 aatagtcttt cagcgacccc caatatgacc gtaactcaaa gtgagcatct cgaggttcac
    43861 gaagaagaaa aggttaattt tttacgctcc ggattaaaat ggagcggaga aaaattcaat
    43921 acctcgggtt caaatgtttc ggtaattctg actatgccaa aagttcgtga tagttacgtt
    43981 ggtgtaatga caatctgtac tcgcggagcg gttgatgaaa ttttacatta ttttaaagct
    44041 tatttgaaca atagtacgac gccggtcgcc agttggtcag ccactaatta tcaaagtgcc
    44101 actacttctt tttcaacgac tctttcagct aacataaata ttttgaaaat taactatact
    44161 tcccaaccta gtagcgccag cgcttatctg gattatgtaa tgtttcgtta tactaaagac
    44221 ctaagcgcat tcactgaacc gcaaactttc tggtcactgt tgtattctgg aatagtcgaa
    44281 tatcaaatta ccgatctaaa ttttaccaat ctactgattc tggacgttag cgactggagc
    44341 aatgtctcca tccagcaata caaccgaaat tctaacctaa tttcattccg tacagccagt
    44401 caatccaatc agcgtcggca gtattatctt accgctccat ctttttttaa taaaccggaa
    44461 aaaatcgagt taatcccgaa tcctcaatgg attacattgc gcaatataac cagttcctac
    44521 gattacatca ttatcactgc cgatgatttc ctgaccgcgg ctgagaaaat tgccactcta
    44581 tattcacagg aactttcccc cggagaccgt cttaatacat tggttgtcac tcaaaatcaa
    44641 atcctgcgcg aatttaatgc cgacatcacc gatcctcacg ccattcgctt ttttctgaaa
    44701 tatgcctatg aaaattggac acccgctcca gcgtatgcac tgttacttgg cgacggcact
    44761 tttgattacc gcgccatcga atccagcaca ggaaattatg taatgaccta tcaggttgaa
    44821 ccggctgtag aagattactc cggtttttct tcctatgctt ccgatgctcg ttttgtctat
    44881 attaaaggtt cggatcaggt tatggatatg gcaatcggtc gggtgcctgt gagaactgct
    44941 acggaagccc tcgattatta tgaaaaatta aaacagtacc ttaccgaacc aatttacggc
    45001 gaatggcgca gtcgaatcac actggttgcc gacgatccgc aacgacctta taacaatgaa
    45061 aaatatcata taaatgatac tgaatcttat attgccaaat atattcctaa aagtctcata
    45121 accgataaat tgtatttact tgaatatcca gaagtccagg atgtatccag taattgggta
    45181 aaaaaacctg ccgcgaccga agccatctta aatcaactca aacgcggcac aactttgatt
    45241 aattatctcg gccatggttc ttccacagtt tgggcgcaag aagaagtact aaaaatggac
    45301 cgcgacattg gtttaatcga agccggaatg aaattaccgc tctggatcgc cggaacctgt
    45361 agctgggggc aatttgacga catcaatact atttgtatgc cggaagcctt aatcaataaa
    45421 aatagcgacg gagccatcgc tatccttaca ccaactcgac caactactgg tactcagaat
    45481 gccagtttca taggcaattt catcaaaaag ttatttccaa gcggcgaaat cagtcgaact
    45541 agacttggca ctctaattca aaatatttat aatggttcta ttgaaaatca ggagaaatac
    45601 gttctattcg gagacccagc cctttacctg gcgttaccgt atcaaaaagc cgctttcgat
    45661 aaaatgcccc gcgacacttt agcggcattg gaaaaggtca gtgttaccgg acgaacagtt
    45721 acggaacaga acttcaacgg cgacggtatt atcaacgttt ttgacagtga ccgttacgtg
    45781 acccgctatt acctcgacga cgctaaagta acccagaatc ttagttacac tctttctggt
    45841 gaaacgctct atcgaggcaa tcttaaaatc gaaaatagta aatttttctc ccgcttctac
    45901 atcccgaaag acctcagtta tatgaacctt ttcggccgga ttaaggttta cggctggaac
    45961 acttctgcta tgagtgattt tgccggttgt tatgattcat tattatttac cggtaccacc
    46021 caattcgatg ataccagtgg cccgcaaatc gtgatcggct ttgaaggtat ggattttaaa
    46081 agtggagaca tcgtcgctcc cgatgccaaa attaaagtat caattatgga tccagtggga
    46141 ataaacatcg ccggcaaact tggacacgat atcactttcc gttttgataa tgataatgac
    46201 cttatttttg aaatgacgga agactttgtt tatgatgtaa atagcgactc ttcaggcgtt
    46261 atcaccagtg acttgcctga tctctcaccg ggaatccacc aaatcactgt aaaagcctgg
    46321 gataatgcta ataattatgc aactgccata gctgaatttg tccttgccag ttctgataaa
    46381 ttgaaattgg aaagagtagt caatttcccc aatcccttcg ctagtacaac cgactttgtc
    46441 ttttatctta ctcagccggg acaggtaaaa atcaccatct acaccatccg cgggctaaaa
    46501 ataaaaacca tagcgagcga tgaaatccta ccacctggtt tccatcaagt gaattgggac
    46561 ggatgtgacg atttcggtga tgaaatcgcc cgcggcattt acctctataa agttcaagta
    46621 cagtcatccg attcaaacca aaaagatacc ttcatcggaa aaatggtgaa atcatgacga
    46681 cccaactcaa ttggtattcc atgccggttg ttttggcttc tcaatcgccg cgccggaaag
    46741 aaattttaga catgattgga ctgaaatacg aagtctgtcc tccccattat atcgaaaaca
    46801 attcaattgg caaatcgccg acaaccatcg ttataaaaca tgccctgaat aaagcccaaa
    46861 ccgtagcacc tcgctatccg aaagcttgga ttatcggagc cgatactgtc gtcgtttgta
    46921 aaaacagaat tttaggaaaa ccacagaacg aaaacgaggc agttcagatg ttaaaattcc
    46981 tgagcggcgt cgaacatagc gtcttcaccg gatattgcgt tctgaattcc gcaaatggtc
    47041 gttatttgaa aaatgcagtg cgaacccggg tcagatttaa agaaattcct ttggcggaaa
    47101 tccaatatta cgtcgaaaat tatcatccgc tcgataaagc cggggcttac gccattcagg
    47161 attttagcgc tattttcgtg gaaaaaatta acggttgttt ttacaatgtt gttggttttc
    47221 cactaccggc tttctatgaa caggttaaaa atgaactttc tatctgcctc tgaaagtttt
    47281 tattgaaatt aataggaatt tttctgatat ttgcaacaat ggaaatgaac ttgaggaaca
    47341 atggcaacgt tttatgaaat attaaaacaa agcagagaag cacagggact taccgttgag
    47401 cagatttcca gccggaccaa aatcagcaaa gaaataatca cagccatcga atccggtgat
    47461 ctaaaaatat tgccgaaaac atatatgcgt ttattcctga aagcctattc tcaggaatta
    47521 aatttagacc ctgaagcaat tcttaaaaat ttcgaagaat cgctcggcga accagttgag
    47581 acaccggcag ttccagacaa caacattaag ccgagtttac ccatttcaca aaagccggaa
    47641 aaaccagaaa aaccaataat aggaaaaaaa caaaaccgca atttttctac tgtaataatc
    47701 gtttttgtaa ttctcgtttt tttgattgca gtcctcagac aggttttgat ggatgaaaaa
    47761 aagacgagca gtaatactgc actaccgaaa attactccaa ctgacacaac tcaaactatt
    47821 cctccgagtc ccgatctcga agtagcagtt cccaaaagcg aattgaattt aactattaca
    47881 actaaagaca gctgctgggt gcggattgtc annnnnnnnn nnnnnnnnnn nnnnnnnnnn
    47941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48001 nnnnnnnnnn ntcattttaa tttctcctct ttttaaataa cccattaatc ctcttaaaac
    48061 cttttaaaaa aactcttgac aattagtttt ttttgtccta ttttgtgggt gaatgtggta
    48121 agaaatccca aataatccca aaaatgcgag gaaactgtaa acccgatgat tactgccttt
    48181 gccggtgaaa cccgccaccc acttgacgaa aaaaatcgct tgagtattcc ggctaaatac
    48241 cggcgttggg ctgatggtca ggacggcgag tgtacctttg tggtaaccaa aggctcggat
    48301 ccttgtttag ttgtctatcc atccaccgaa tgggatgtat tatctgagaa acttttgaaa
    48361 ttatcctatt ttaagaagaa aaatcgggct tttatccgag cgctggcacg tagttctgtt
    48421 cgtctaaagt gtgatggcca ggggcgtatt ttgataccta caagtttact tgaatacgcc
    48481 catattaaaa aagaagtaac gatcatcgga gtattgaatc gtctcgaatt atgggatccg
    48541 gaaacacttg aaaaacatga agagtcttcg attcctctcg atgatgaata ttacgaagac
    48601 ctgggcgaca ttctttaatc ttgatatttg gaaaattatg gtatgactac cctccatatt
    48661 cccgtattgg ttgaacaaac aatccaattt ttgatcaata gaccgacggg aatttacgtt
    48721 gactgtaccc ttggtaccgg tggtcatttc caagcgatag cccaacattt agataatgac
    48781 gcatatctaa ttggtataga tgccgatccg agtgccgtgc aatattgcga gcaaaatatt
    48841 tcgatttctc agcctcacca gttcatcact gccaattttg ctgatattaa acggatctgc
    48901 tatcgggcag gctttccgaa agtcgacggt attttaatgg atctcggatt atcatcattt
    48961 gccttagaca atcccgagcg gggttttact tatagtcagg ctggtcctct cgatatgcgc
    49021 ttctcgcctg atactgctca gactgcgatg gactttatca atacggctcc catgaaagaa
    49081 ctggcattag ttttcaaaga atatggtgaa gaacgttaca gctacaatat tgctcgtcgc
    49141 ataataactg aaagagataa aaatcctatt aaaactactg gccaattagc ctctattatt
    49201 cgggaagaag ttccaaagca atttcaaatc aaaaccttga gtcgtatttt tcaggcgatt
    49261 cggattcaag tcaatcagga attagacgct ttacgacaag gtctatcaga tgctgtttca
    49321 ttattagctc cttccggacg gattgttgtt atttcttatc attccattga ggatcgcatc
    49381 gtcaaacaat tttttaaaaa ggaagccagc gattgcatct gccctcctga tttcccaatt
    49441 tgtcaatgta atcaccatgc ccagttaaaa attttgactc atcgcccgat agtgccgagc
    49501 aaatctgaaa tagaaagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    49561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtt
    49621 gttatagatt ctaaagtttt agcagttgaa taatggctaa tcaatttaaa tccattcgga
    49681 aaaggacttt gatagtgagt agctcattag tggttctctg ggtggttttg atagcacgcc
    49741 ttttttatat tcaagtttta aattcaaaaa aatatcagga attatgtaat aatcaagcca
    49801 atctgcgcaa agccgtgtta ccgcgccgag gcatatttta cgatcgcaac ggcgaagctt
    49861 taaccattga tttagttaat tactcgatcg cggcgcatcc ctacctggtt gaagataaac
    49921 cccgtttagc cagtactctg gctaatgatt tgagcgccaa acccgagcag tatcttaaac
    49981 tgttggaatc caataaaact ttcgtctggc tggaacgcga agttccctac tcaagttttc
    50041 aaaattatga aaaatatgaa aagactcccg gattggttat cgaaaaaaat gttaaacgct
    50101 actacccgct cggtgaaatt accggtcaat tgcttggttt tacaaatata gataatcgcg
    50161 gagtttgcgg gctggaaagc ggattggaag gtttactttg cggaacgccc ggctggatta
    50221 tggttcaaaa agacggctgg ggacgacttc actatcggcc ggatttgcct tccaaaagca
    50281 gtattgacgg caatgatatt gtcctgacaa ttgatcggga gtatcagact attttatatg
    50341 aggaactttt acaggcttac accgaaacaa aagctgaaaa agctatgggc atcgtgatta
    50401 atcccaatga tggcaatatt ttagctatgg ttaccttgcc acctttcaat tctaataaac
    50461 cttccgatta tccgatttct gctcaacgca atgccgtagt ttcggatata ttcgagccgg
    50521 ggtcaatttt caagattgta accgcgacag ccgctctcga taacaaagtt ctgcaaccta
    50581 aacaccaaat tgattgtaat cccggttata ttcaagtcgg gaatcggata attcgcgatc
    50641 atgataaata cagcattctg actttcgcgg aagtgataaa aaatagcagt aacgtcggaa
    50701 ccattaaagt cgcccaatta accggaaaag atcaaatctt taactatgct cgcaagttcg
    50761 ggttcggggc aaaaaccgat atccagtttc ccggtgaaga atccgggatt ttacacccat
    50821 ttaaaaaatg gaacgaccta gttctggctc aggttgctat cggtcaagga gtttgcgtca
    50881 cggctttaca aaccggaatg gcttatgcgg ctattgccaa tggcggctta ttacttaaac
    50941 cgcaaatcgt caaaacgatc aagaccaaag atggcattga aatcttcaat gctcaaccgg
    51001 tttatatcag gcgagttgcc agtagtgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    51061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    51121 nnnnnnnngg tggtggtgtt gcggcaccgg tcgttcgaga agttttcaaa agaatcgtga
    51181 accagtctga cgatcttttc tttgacgaac ctgccccttt accaactcca caggctaatt
    51241 atgcttatga aactaaaccg gtagtcgcta atgttcctca aacatctgac gaggcttatc
    51301 atcccaattt aacgactgcg atttatacca atcaaatgcc tgatctttgt ggaaaaactc
    51361 ttagacaggc ggttggaatt ttgtatgaac tcggaattga cgatatcgaa attgacggtg
    51421 cgggagtggt ggtcagccag aagccagcac ctcatgcgcc aatcaatccg catgtcaaat
    51481 gcatattgaa acttcaaccc actcggacgc ctcttgaata attcactttc cgaaatatta
    51541 caaggtattg aatttcgtcg gctgggaaac ttaccgtctt cggtagcggc tcttagtgtc
    51601 aattccaagg aattggctaa gggggcgctc tttttcgcaa ttcgcggggc taaggatgat
    51661 ggccataaat atgttaaaaa cgcaatacaa aacggtgcag ccgccgtcgt ggtcgaagaa
    51721 gtagtcaatg atgaatccat ccctcagatc atcgttgccg atactaaaac agccctctcg
    51781 aaagccgccg ccaattttta tgaaaatccg accaataata tgacgatcgt cggtgtaacc
    51841 ggcacaaatg gcaaaactac caccgtttac ctattaaacg aaatattcaa agaagccggt
    51901 ctaaagcacg gtactgtcgg cacgctgggt tattcgattg gagatgaacg tttctcctcg
    51961 aacttgacta cacccgatag tattcaatta cagcaaattt ttaaaaaaat gctcaatcgc
    52021 ggagtcgaaa tagcggctat ggaagtgtct tcgcatgctt tagccttgca tcgcgttgac
    52081 gatgtccatt ttaaagccgc cgtgtttacc aatatttctc aagatcatct cgattttcac
    52141 catagtctcg atgaatatgc cacaactaag agcaaactct tcgcaatgat tgaaaaagac
    52201 gggtttgtaa tcaacaatct cgatgataat tattccgatt tattcaagaa cgccgctcga
    52261 tgccgcctgt tcacctattc tttgctgaag acagccgatt tcacctgggc gcccggagta
    52321 acctggtcaa acggcatttc cggagaaatc ctaactccaa acggtaaaat tgaaatcaag
    52381 agtcgccttt cgggacaatt taacttgaaa aatattttgg cggctacggc ggtagcggtt
    52441 aatctaaata ttgacgataa aatcatcgct tcagccctag acagagtcaa atctgtgccc
    52501 ggccgcttac aggaaatttc gcatcctggc caatttcgcg tttttataga ttatgctcat
    52561 accccggacg cgattactaa tgtcttgcag actctacgtg gaatgcttcc ggccgacggg
    52621 cgcttaattg tagtttttgg ttgtggtggt aatcgcgatc gcgataagcg tcctaagatg
    52681 gcacgagctg tcgagtcctt agccgacttg gcgatcgtca ccaccgacaa tccccgcttc
    52741 gaagaaccgc atgatattat taatgatatt gttaagggct ttacgagcgg gtacaatttc
    52801 aaagtaatca ttgatcggaa agaagcgatc ctggatgctt taaaaaatgc tgagcaaaac
    52861 gatataatcg caattcttgg caaaggtcat gaaacctacc aagaaattcg cggagttagg
    52921 tatccatttt acgacgggga aatcgtgcaa caattctacg gcggatcaaa tgattaaagc
    52981 agatttcatt cggtcactcg gtctcggacg catagtcaat ttcgaagggg caatcaatgg
    53041 cgcggaaatt gacagtcgca aacttgagcc tggtgcggct ttcttcgctt taaagggtgc
    53101 gacatccgat ggtcatgctt atgttcagga ggctttgcag aaaggtgccg ttttgtgcgt
    53161 ggtagcggaa gattgggcgg ctaaaaatcc gactcccgca cctctctgga tcgtacccga
    53221 tccggaaaaa gccctccaga aattagccga aaaatggcgc ctctccttct ccattccggt
    53281 gctggcgatt actggaacta acggaaaaac aacaactcgt gcgatgtgcg ccgcaattat
    53341 aaaaacagcc tataacttgc atacaacttc aggcaatttc aataatcaac tcggtttacc
    53401 gatcactttg ttgaatttaa gacctgaaca ccaattcagt ctaattgaaa tggggacaaa
    53461 tcactacggc gagattgccc gactatgtga aattgcccat ccgaatgccg gtttaattac
    53521 caacgtcggg tggggacata ttgaattttt cgggaatatc gaaggtgtag cgcaagcgaa
    53581 atcggaattg tttgccgcct tgccggaaaa tggcaccgct tttattaatg cagatgatcc
    53641 tctaattgtc aaaatgccgg tagccgggcg caaaattaag tacggttttg aaaataaagc
    53701 tgttgatttc tatggcaaaa tctcaggcta taacgatgaa ggtcaagcag ttttaaacgt
    53761 caattcgcgg tttgacattc atctgtcaat tcccggtaga attgccgctt taaatgctct
    53821 ggcggcggtt gcagtcggtt tattttttaa tgtagatgaa aaagcggcca tagcggctct
    53881 tgaagcattt caaccggtca gtcaacgctt tgtgcagatt aaagtcggtc cctatcaggt
    53941 gattaatgat gcttacaatg ccaatcctga ttcgactatc gccgctttgg aaacctttgg
    54001 acggattaaa acttctggac gacgcatttt cataatgggt gatatgctcg aattaggtca
    54061 atattcctct tcagggcatc agcgtgttgg acaggcggtt gctaaagttg gcattgattg
    54121 cttttatggc gttgggacac aaactgctta tgccaccgaa gccgccgctc aggttggctt
    54181 gaaaaacgtg tatcatttcc agaacaaatc tgatttatta tccgctttga aatccgattt
    54241 gaaaaaaggc gatactctac tcgtgaaagg ttcacgtggc agtcgtatgg aagaaattat
    54301 tgagggacta agccaataat atgttttacc atctcctcta tccattaaaa aaatacttca
    54361 caggatttaa tctgtttcag tacatcacct ttcgagcggc tatggcggct attacggctt
    54421 tgctgattag tttcattatt ggtccataca tcattaaaaa gctaaaaaaa taccaaatcg
    54481 gcgagttgat tcgggaagat ggcccccaaa cacatttggc taaaaaaggt acaccaacaa
    54541 tgggcggaat tattatctta ttttcggtaa ttgttccagt acttttgtgg gctaatctta
    54601 ccaacaccta cataaatcta attttactta gcacagtctg gatgggtatt ttcggtttcc
    54661 tcgatgatta tttaaaagtc gttaagaaaa agaaaaaagg gctgatcgct cgttataaac
    54721 ttttagggca aatcgctctg ggtttgttaa tcggttgtta tctgtatttc tcccctgaat
    54781 ttgctgacat taactcaatc acttccgtac cgtttttaaa aaataattat cttgatttcg
    54841 gatggtttta tattccgttc gtaatttttt tcatcactgg tatttctaac gccgttaatc
    54901 taactgacgg cctggatggt cttgccgctg gcttgatggg aattgtcgcc ttggcgctgg
    54961 cggtcattgc ttatataacc gggcgcgtag attttagtaa ttacctgaac acgatttatt
    55021 tggcggggag tggggaatta tcaatattct gtatggccgc cgccggagca tttcttggtt
    55081 tcctgtggtt taatgccaaa ccggctgaag tttttatggg cgacactggc tcactttcaa
    55141 tgggtagtgc cctggctact ctggcaattt tattgaaaaa ggaaatattg ttcatttttc
    55201 taggcgcttt gttcattgcc gaggcgctct ctgtcataat tcaagtggtt tactttaaat
    55261 ggactaaaaa gcgaaccggc gttggtcaac gggttttcaa aatggcgccc ttacatcacc
    55321 atttcgaaat gcaaggcata ccggaaagta aaattgtaat ccgcttttgg ataatcggca
    55381 ttttgttggc tttgttcagt ctaagtactt ttaaaattct atgatagatt taaaaactaa
    55441 accagctaat ctgaatgatt taaaagtcgc cgttctgggc gctaaacgca gtggaattgc
    55501 ggccgcagta ttagccgccc gacggggtgc tgaggttctt ttatcagata ccgccaatcc
    55561 ggaaattgcc ccagaacagc attccgcatt aaagcaacac aatgttcaac tggaaattgg
    55621 cgggcacacc tcgaaaattt ttgattccga cctagttgtt ctaagtccgg gaattcccaa
    55681 tgatgctccc atcgtcattg aagtcaccaa acattcaata ccgatcgtgg ctgaagtgga
    55741 gatggcttac tggttctgcc ctacggataa tatcatcgcc gttactggtt ccaatggtaa
    55801 aactactacc actacccttc tatctgagat ttttaaaggc actcaatacg aaccgtattg
    55861 tggcggcaac atcgggacgg cttttagcca tttgatcatt gaggctgaac gttccgccca
    55921 cccccagaaa attttcattc tggagctgag cagttttcag ctggagcgca ttgtccattt
    55981 tcgtccgaaa gtttcgctga tcctgaacat aacgcccgat catctggatc gctacgaaca
    56041 tcgtatggag ctttatttag ccgccaaatt gcgcataacg atgaatcaga ccgaagatga
    56101 ctactatatt tttaatgacg atgatgaatt gctaaagact aatttgccgc cgagatgcca
    56161 tttgattccc gccggattac attccacttc tcagaaatta attacttcag acgccgagaa
    56221 gatttatttt tccggacaag atcaattcat tgaacgggcg cgagttgctt tgctcggcga
    56281 acataatctt tacaatttga ccgccgcttt gactgccggg cgattgtatc aattaccaat
    56341 tacgcattta caaaatgtat tgtacaattt caaaagcatt gaacatcgcc tcgaatttgt
    56401 gacaaccata gacggcgtgg actattacaa tgattctaaa gccactaacg ttgattcggt
    56461 caattatgcc ctaaaaagtt ttaaccggcc ggtaattgtc attttgggcg gaaaagataa
    56521 agactccgat tttacgcttc tcatcccgca aatccgggag catgtcaaag ccgcagtttt
    56581 aattggaaaa gccgctgata aaattcgcaa ggcattgatt ggtgtcgtcg agatgtatga
    56641 cgccgggtat tctatgccga atgccattga aattgctcgc aatctggctg tgcctggtga
    56701 catagtcttg ctctcgccgg cttgtgccag tttcgatatg tttaatgatt atgaacatcg
    56761 cggtcgggtt tttaaggaaa tcgtgatgaa tttgccgaag gacttgaaag tatgaacgtt
    56821 gcttttaaaa aaggaaaagt tgaccaaaaa tacttgatcg ccttttttat tctgttaggt
    56881 tttggtttag tgatgcaata tagtgccagt tcttcgcttg gggaagcccg ttttgacgat
    56941 ccgtcttttt atgttcgtgg acagtttatt cggattgtgt tgggtatggt tgttggtctt
    57001 atttgtactt ttatcaatta tcaaattctc aaaaaaggcg cgttctggat tgctctggct
    57061 gggattattg cactgattgc gactttagtc tatcacaaat tgcaccccgg tcaagcgact
    57121 gcgcgctggt ttccgatcgg cggttttaat tttcaacctt ctgaattcgc caaattggca
    57181 ttaattattt acctttcatc cttcatcgag cgccacgaaa gacatctgga tgatttcaag
    57241 cgtggtttta taccaccagt catcattatg ttaataatgg tgatattgat tatcgtcgag
    57301 ccgaattata gcacagctgc tgtttttact gttttcggtc taattgtctt atttatcggt
    57361 ggcacgaaac tgctccactt tctacccttt ataggtggat ttgcattatt atcggtaatc
    57421 acaattctgc gttcaccgta taaactgatg cgcatcctga cttttctgga accagagaag
    57481 gatttacagg gcgccggtta tcaaattcat caatcaatga tcacactggg taatggtggt
    57541 tttttcggtc gtggtctggg tggcagtatc gagaaaaatt tgtttttacc tgatatgcat
    57601 actgatttta ttttctcagt agtcggcgaa gagcttggtt ttatcggggc ggtggttttg
    57661 ctatcacttt acctttatct atttatcagg ggaataaaaa tagctctgcg cgcgccggat
    57721 gttttcggca atttgctagg tatcggtcta tcgagttgtt tgtttattta cgtaattgtt
    57781 aatgtgggtg ttacctgtgg acttttaccg gtaaccggct tgcctttacc cttcataagc
    57841 tacggcggtt cttcgatgct attcaatttc gcctgtgtcg ggatcattct aaacatttca
    57901 cgctatgcgg tcccgcaaga aaaaacaaat tcactggtga tgttcaatga ctaagaaaga
    57961 tgaatcccga cgtttaaaag tattaatcac cggcggtggt acaggtgggc atctttttcc
    58021 ggcgatcgcc ctagccgaag aactccagat gcgtcaatcg cagattggcg aactcgaaat
    58081 catttttatt ggaacgcggc ggggcttgga atctcaaata gttccgagtc tgggttttga
    58141 attccacaaa atctggattc gcggctggca acgcggctgg actctaaatg atattttggt
    58201 caatctgtta tttcccgtca gattattcgt cagtttgata caatcatttt ttatcattaa
    58261 aaattttgat cccgatttag ccattggaac cggcggctat acggctggac cacctctaag
    58321 aattgccgca atgctgaaaa tcccgatttt tctccacgag cagaatgtca ttccaggggc
    58381 tacgactaaa atgctggcaa acaaagccaa gcggctttat acgtcttttg aagagtcaaa
    58441 aacttacctg gaaaacgcca tctgcctcgg cactcctctc cggcgttctc tgcaaattgt
    58501 tccgcgggag caggcgattt acttttttga attggagccc gatcgcaaga ccgtttttat
    58561 ttttggaggc agtcagggct cccaagctat taattcttat tggattgaga atatcaccac
    58621 ggtggtggaa gccgttaaat gtcaattcat ctggcagacc ggacagcgcg attttaaaat
    58681 gattgataac atttttagta acaatccctg ggttcacgtc acgcctttca ttcatgaaat
    58741 gggaattgcc tattgtgccg ctgatgttgt cattagccgg gcaggcgctc tatcgctggc
    58801 agaactttgt ctctatggca aaccttccat tctcgtgcct ttaccaactg cggctggcaa
    58861 tcaccaggaa atcaacgccc gcaatatgga acagcgtggc gcttccattt tggtactcca
    58921 gaaagaattg actaacgacc gattgactca ggaacttatc gctttactga aagatgagcc
    58981 gcgtcttcaa gctatgtctg ccaacgctaa agccttagcc agaccggatt cggcaaaatt
    59041 aatcattgac gatattttag gttttatcga agtaaatgca ctctgaagtt aaaaaatatc
    59101 actttgttgg aatcggcggc ataggtatgt gcggtctggc tgaatattta ttgcacaacg
    59161 gtcaaattgt aaccggctcc gacctggcgc cttccgaaaa tacggaacgg ttggaaaatc
    59221 tgggcgctca aatcaaatac ggacatcgcc gcgaaaacgt cgacgattgt gactatctgg
    59281 tatattcttc ggccgtcaag ccggacaatc cggagagaat ggtcgctatc gaacgtcaaa
    59341 ttccaactct gcggcgagcg gaattgctcg gccaaatctt tgatactaaa cccattcgga
    59401 ttgcagtcag tggtactcac ggaaaaacga ctacgacggc gatgatcggg caagtcttga
    59461 ctacggctga gctggaccca ctcattttaa gtggtggcgt tttgaagagt agcggtagcc
    59521 cggttcaaat cggcaaaggt gatatagtaa tcgccgaagc cgatgaattt gatcgcagtt
    59581 tcctgcaatt atctccaacg catgccatca tcacaaatat tgagagtgaa catttggatt
    59641 gctatgcaaa cctcacccaa atcaaaaatg ccttttatga atttaccgca aagataccgg
    59701 atgctggcag agtcatcgcc tgctcggatg aaaaagaaat acccgaattt ttaaaacatc
    59761 atccgcggcc atatgttacc tatggtcttt atagcggaga atttcaagct cgtaatatcc
    59821 aattaaaagg gaattcaagt gagtgcgaag tttatcataa ttcagattta attggctcgc
    59881 tccaattaca cgtccccggt ctacataata ttaaaaacgg actagcggcg atcgcgatgg
    59941 cttctttact aaacataccc ttcccggtta tccaggaagg tttggcagac tttcggggcg
    60001 taaaaaggcg attcgaaatt ctacaagaaa acgacaatct tatgctggtg gatgattatg
    60061 cccatcatcc cagtgaaatt gcggcgactc tcaacgccgc tcgcaaaggc tggcaaagac
    60121 gaatcattgc ggttttccag ccccatttat tttctcgtac tcgggatttc tatcccgaat
    60181 ttgcgctggt acttcgggtg gctgatgtta tcgttcttct tgaaatttat ccagcgcgag
    60241 aagcaccttt agaaggtata agttcccgct tgatttatga tgaaattcgc aaagaccgga
    60301 ctcaggacgt ttaccttacc aacacactcg atgaagctct ggcattattg caagaaataa
    60361 aattaaccgg cgatatgatt atcacactgg gcgccggtga cgtcaataaa attctactga
    60421 aattaatgga ataaaaaatt gaaactggga ttgataatat taaaaaataa atcgcaaagg
    60481 tgtttaggtg ttcagaattt atcttctttt ttcagaaagt actgggacct aaatagactt
    60541 tgtgatcaaa acctttggac aaaaattttc cagggttccc gcagatatgg ggggcaaaac
    60601 atcaaaacgc cggctcttca gcaccgaaaa atttctgcaa tcggttatcc cacgaacaaa
    60661 gatttcgatc tgaagacaca gcaacgccag gtaattttgc tattattctc gggaaccctg
    60721 aaaggttttg gattaactaa ttgatgattg atgaaatgac agtagaaaaa gcaactaaaa
    60781 tcagtcggaa tgaccagaca aagcgtccta gaaaaaaagt gtggtttttt ctctgtttag
    60841 cattgattgg cggatttagc tacctcgtat atttggctgt tgactggtgt gaatatacta
    60901 atttctttaa tctcgataag atcgaagtca gcggagtatc aattttatct aatgaagaaa
    60961 taataaaaat cgctggtctt aagaaccacg ttagcctgct taaattagac attcatcaga
    61021 ttcagaaccg gcttgagatg gaaccatata tcgaggcggc agtcgtcagt cgcgaatttc
    61081 cgaatcactt gaaaataatg atcaaagagc gcatcccagt ttgttacttg aataccggca
    61141 cgctctggct aattgaccaa caaggaatca tattaccgct tcgcaaggaa aaattaaaag
    61201 ccaatttgcc ggttattacc ggtttcgaga aagattccgc ccgttgtcgg ccgggacagc
    61261 ttgcacctaa tcccgaaatg ttcaaagcct tgaatttcat ccagactgcg gctctgaagg
    61321 ccaatactct gtataccgaa atttcagaaa tctttgcctg gaaaaagggc gaatttatta
    61381 tttatacggt caacggagga acaccgattt atatcggacg tggtgattta accaaacaat
    61441 tatatattct ggcggctttt caaaacttaa tcaaatccaa gcgcactttt gccgattatc
    61501 aatatcttga tctgcgctgg gataaacaaa tcatagcgag agaacgaaaa tcataaatga
    61561 aaatgttaaa actaaaaaac caaatcctga ccggtctcga cgttggctca tctaaagtct
    61621 gcgccgtaat tgccgaagtt actcccgatt cacccgaacc tattattaaa gggtacggta
    61681 tcagtccttc taaaggaatt aagaaaggct tggtcgttga tattgaaaat gcgactcagt
    61741 caattgttaa tgccgttagc gaagctgaaa aaatggctgg tttttcgatt aattcctgcc
    61801 tggtaggagt ttcgggcgat catatccgca gtttgaatac acgtggaatg atggcaatct
    61861 cgcgcgattc acgtaccggc ttaggcgaag cccgagaaat cgagatcgaa gatatcgagc
    61921 gtgtaatcga acacaccaag gctgtaccgc ttccgttaga tcggcagatt ctacatgttc
    61981 ttcctcagga atacatcatt gatgaacagc cgggagtaaa aaatccgttg aatctcagcg
    62041 gtcgacggct ggatgccaag gtacatctga ccacctataa taccaccgtt ctttccaatt
    62101 taagtaaatg catcaagaat gccgatctgg aaattgacgg ctttgtattg agttctctgg
    62161 cggccgccta tgcctgtctg gaagagagtg aaaaaacgat gggcagtgtt cttctggaca
    62221 ttggcagtgg agtcattgac ataatcgttt tttacgatga cggtgttcac cataccggag
    62281 tcgtcaatct gggagctaat agcgttacca atgatatcgc ttatatgctg agagtccccc
    62341 tcgaaaaagc cgaaagtatt aaacgtgaat atggttttgc caaagtctct ttgaccgata
    62401 gagaagcatt tttcagactg gatagtcttg gggggcggtc acctcgagaa atttctattt
    62461 ttaatctggc tgaatggatt gaaccacgta tggaagaaat tttgcgcgaa tcatatctcg
    62521 aagccaaaaa agccgatatt cctttgacta atacattatc agtcgtccta acgggcggcg
    62581 gcgcccgttt aaaaggcacc gatgaattag ctgaaatgat tttcaatacc ccagcaagag
    62641 tggcagctcc ccgtggtttc caggaatatg tcacggaatt aagtgatccg atttattcgg
    62701 tggcaatcgg cttaattaaa tatgcaattg ccggacaaat tagcggttcg gctttaaata
    62761 aaacaatcag cggtcccttt cacagaataa ggggctggat taaacacgta accgaaaatg
    62821 taatgtaatt agggaggcct aaaatgttat ttgaatttga agaattagtc aatcagcaag
    62881 ccagactgaa agtggtcggc atcggtggcg gcggcggaaa cgccattacc agaatgattg
    62941 agaatggact gatcggggtt gatttcatcg ctctaaacac tgatgttcag gcgctcgaat
    63001 gttgtaaagc cccaactaaa tttcagatcg gacgagctct gacacgtggt ctgggtgctg
    63061 gtgccattga tgacattgga agaaaagcgg ctgaagccga tcggcagacg atagcttcaa
    63121 tgattgaaga taccgatatg gttttcatta ctgctggaat gggaggtggc actgggactg
    63181 gcgccgcacc aatcgttgcc caaattgcac gggaacaagg ttcattgacc gtcggaatag
    63241 tcactaaacc ttttaatttc gaaggggcga agcggatggc acgcgccgaa gagggtatta
    63301 atcaactcag gaaatgcgtt gatactctaa tcgtaattcc gaaccaaaaa ttattggcaa
    63361 ttgttgacca cgatacttcc atattggatg tttttaaaac tgctgatagc gtattatatc
    63421 aagcgattcg tggcatttca gacctgatta acaaaaatgg cgttattaat ttagattttg
    63481 ctgatgtcag aaccattatg aaagatatgg gtaaagccat tatgggtact ggtttggcaa
    63541 ccggtgagga acgcgctgtt cttgccgctc agcaagctat ttccagtcca ctacttgatg
    63601 aagttaatat caaagatgcc aaaggtctgc taattaacat taccggaccg gctaacctcg
    63661 acatccatga agttgacgaa gcctgttcaa ttataaccga tgaggttggt gccaacgctg
    63721 aaaccatctt tggtatggta gtggacgaaa attacaccga cgaaattatg atcaccgtaa
    63781 tcgcgactgg ttttggtgaa gcgccgacgc cgattaatct gtcccaaaat aaaaaggatt
    63841 tctcctcccc aattaaagac gaaccacgaa atactgacga tatttggaat agaattaatg
    63901 aaccgggaca gcctgttagc ctcttcagtg atgaccaggt tattaaaatc ctgaatcctg
    63961 atgatatggg tatggctgat aatccttccc ataaaataca ccagaccaat attccaactt
    64021 atatacgtca aaaaataaaa tagttcgatt tatttttctc cccctattaa aatccaaaag
    64081 cccctgttta ttcaggggct tttagtttga atccttagag agagtattaa tatcagacgg
    64141 ctttttgaat tgtaccgctc ttgagacagg tcagacaaac ttttacagtt ttaattgaac
    64201 cactattatc cactattcta attttacgca gattgggttc ccatatccga tgagttctat
    64261 tattagcatg actaacttta ttgccgtgaa tcggcttttt tccgcaaata tcacaaactt
    64321 ttgacatttt cattactcct taaaaagc
//
LOCUS       DAOSMP010000010        63060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_503_length_63060_cov_17.223855, whole genome shotgun sequence.
ACCESSION   DAOSMP010000010 DAOSMP010000000
VERSION     DAOSMP010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 63060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 63060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63060
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_503_length_63060_cov_17.223855"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..401)
                     /locus_tag="PKX09_02900"
     CDS             complement(33..401)
                     /locus_tag="PKX09_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU16805.1"
                     /translation="MKVFIADDSEILRKHLTNLLIEIPGVEIVGYGEDTESAISGIDA
                     QKPDVVILDIRMPGAGGIYVLKTVRQNRPDLRIIVFTDYPYPQYRKKCLEEGTDYFFD
                     KSSESEKLIELVRKMAGGKA"
     gene            complement(626..1069)
                     /locus_tag="PKX09_02905"
     CDS             complement(626..1069)
                     /locus_tag="PKX09_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOU16806.1"
                     /translation="MAITRWTPRSSWLDVRNEFDRLFESIFNPQYEEETSLLAFSPAV
                     DIIEEDKAYNIQVELPGMNKDDIKIAVKDNYLTISGEKKRESKVEDKNYQRTERIYGS
                     FQRSFRLDDSVEADKIEAEFKDGVLNIVIPKKKESLAKQIEVKVK"
     gene            complement(1106..1537)
                     /locus_tag="PKX09_02910"
     CDS             complement(1106..1537)
                     /locus_tag="PKX09_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013554948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOU16807.1"
                     /translation="MAITKATPKSVTRKSPYTIDRFFDPFFDWDTETSLVTFAPEVDI
                     EEKDTEYVVTVELPGVKKDDVKISLKDNVLTISGEKKRVKKINKKNFYQCERVFGSFE
                     RSFRLPELVDHEKIEAEFKDGILTIVVPKSPESKPKSIEIK"
     gene            1871..2248
                     /gene="folB"
                     /locus_tag="PKX09_02915"
     CDS             1871..2248
                     /gene="folB"
                     /locus_tag="PKX09_02915"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOU16808.1"
                     /translation="MGIVRLNNMVFYGYHGVAEPEKILGGKIEVDVELEFDMTKVITT
                     DHLADTIDYEKVYQLVQGVVTQSKFFLIEALAGKILSEIFNHHLVESVRVRIRKPNAP
                     IKGVLDTVEVEIFRRRDQMGEKS"
     gene            2245..2784
                     /gene="folK"
                     /locus_tag="PKX09_02920"
     CDS             2245..2784
                     /gene="folK"
                     /locus_tag="PKX09_02920"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOU16809.1"
                     /translation="MKSSCVFVALGSNLNLREKNLSAALELLSHNPAISICRMSAVYE
                     TAPKYNPDQPKFLNMVVEITTSLTPLQLLEFCKDIEVRLGRHFPHYKNSPREIDLDII
                     FFNRLIVESPTLTIPHPGLYERKFVLVPLAEIAPEFICPKTAKSVAELLRICPDIEQV
                     VRIGQLDELLTIPVTTGVL"
     gene            2781..3425
                     /locus_tag="PKX09_02925"
     CDS             2781..3425
                     /locus_tag="PKX09_02925"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOU16810.1"
                     /translation="MKNLYFLAIEGVIGAGKTSLARLMAEKLNARLVLEKFEENPFLE
                     DFYRDPERYAFQAQLYFLLSRYRQQIELKQIDLFTNLLISDYMFAKDKLFAILNLDEK
                     ELSLYNLVAQHLEKDLPKPDLVVYLQASTDRLISNIKKRGRSYEKSINRDYIDSLNQI
                     YNEFFFRYKDSPLIIVNTNEIDFVNNENDLNELIREIKQPISGTIYYNPAKKIL"
     gene            3425..3622
                     /locus_tag="PKX09_02930"
     CDS             3425..3622
                     /locus_tag="PKX09_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16811.1"
                     /translation="MLRLLLLFLIFYLLWKMVKPRNLRQPKPNNPKPKKPRTTLKIDK
                     SQIEDAKFREIKEEPEKEPKD"
     gene            3636..4265
                     /locus_tag="PKX09_02935"
     CDS             3636..4265
                     /locus_tag="PKX09_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16812.1"
                     /translation="MTISVFFLNILIISSVLILVFFIFGQTPDDKPSGWRTLIAEHLN
                     RYPAMQPADVYKLVYQGTFGPAHLGNDSIQISTSLENELAIITPDSSAPLSESITPSG
                     KYVRLNLKRCKAEQIQTAKIVTAILNSIPQSSADSSRFIRRWETIEKLVVEGSLPIPR
                     SDFFTFSDSIKKTGYPVIHHSSQYIKNYQPAYRVIDRRYLNLISDSLSQ"
     gene            4351..5256
                     /locus_tag="PKX09_02940"
     CDS             4351..5256
                     /locus_tag="PKX09_02940"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU16813.1"
                     /translation="MKKLVITGASGFLGGHIANLAASRFQTLGLYSTYPFNLKGITTQ
                     RIELSSPSSIIVVLKRFKPAVVIHNAALANPDQCENQPDFARQNNVIATEHIASWCHK
                     NNARLIYVSTDMVFDGQKGNYSETDRPNPLSVYGKSKYEAEKLAMANNPDTVVARLAL
                     MYGRGIFPRAYNSEWLLRELLRRKKEPKLPPLKLYNDQYRSMLMVTNAAQALIELALS
                     HFCGIIHIGGPERISRFAFGEKLCRKLNLPLTFIQAVPYDEHQLTAPRPRDLSLDISL
                     ARSFLKTNFLAVDDGLDEMLMSNSD"
     gene            5268..6263
                     /gene="dusB"
                     /locus_tag="PKX09_02945"
     CDS             5268..6263
                     /gene="dusB"
                     /locus_tag="PKX09_02945"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOU16814.1"
                     /translation="MDSVQKITALTIGKVCIKPPLILAPMAGYTDQAFRQICKANGAG
                     LVCTEFVSSDGLVRSSEKTYDYLRFSDFERPISMQIFGNNPVVMAEAANLIENEFHPD
                     LIDINAGCSVRKVIKKNAGSALLKNPPLLEKIARAVVEAVSVPVTVKMRSGWDRQSII
                     AVETAQRLEQTGIKAITIHPRTAVDGFRHPADWSLIKEIKKKISLPVIGNGDVFTPTA
                     ALRMFTETNCDAIMIGRGALENPWIFRQISDLLEDKPLISYDSPTERVKLCRRHLEME
                     IQYRGEVVAHKEMKKFYRWYLRGFGHVSEIREKLVRSKSVQEAFNILDELAHVTK"
     gene            complement(6321..6908)
                     /locus_tag="PKX09_02950"
     CDS             complement(6321..6908)
                     /locus_tag="PKX09_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16815.1"
                     /translation="MKKTLVVFCVVVILILASLATAGTPTTQKGSAAMWFNFNGLSDM
                     SYQNSAIGLNYLFKDKTGAGIALNFSHDSEDDGIEEKEKTTALGMDLYLIHYPFQKGP
                     VALYLAPGVGFNFSSKKDKDFGDIHINSTYMWLGGSVGVEWWIFDQVSLSASNWVGLE
                     IDLLNDKENDDKMTDVHLGILGSSTASIVLAFYFK"
     gene            complement(6947..8125)
                     /locus_tag="PKX09_02955"
     CDS             complement(6947..8125)
                     /locus_tag="PKX09_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOU16816.1"
                     /translation="MNIEQIQTEIRQQNVDGWLFYDFHNRDKMAYHILGLNPNMFVTR
                     RWYYFIPAEGIPIKLVSAVEATKIDNLPGEKIIYRSWEQQHNSLRTMLKNSRRIAMQY
                     SPNNNIPYISVVDGGTIELIRSFGVEIVSSANLVQIFEAVIDEEGYQSHLRAGQKVQQ
                     IKNEAFALISSRIKEQKSVTEFEVQQFIVKRFGEEDLTDDNEHPIVGVNEHPADPHFE
                     PKAENAYTIKPGDTVLIDLWAREQSERAIYYDITWCGFVGQNPPAKYVEIFNIVVRAR
                     NAARDFIRRKMADNQPCYGYQVDDACRNVVKEAGYGDYFVHRTGHSIGHYVHGNGVNI
                     DNLETKDERELVPGICFSIEPGIYLPGEMAVRSEINVFITKDREVVVAGDEQEKLLLL
                     "
     gene            complement(8137..8679)
                     /locus_tag="PKX09_02960"
     CDS             complement(8137..8679)
                     /locus_tag="PKX09_02960"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOU16817.1"
                     /translation="MKLKNERSVLVIIDVQTKLLSVMYNYPLLLENLKKLIRGSQVLD
                     IPIIYTEQYPTGLGPTHPEIASLLSNTPPIIKMAFSCCSEDNFMNEIHKINRDQIIVC
                     GIESHICVYQTCRDLIDKKYEVHLVTDCIASRKPENTQLTVSKLLAYGVHPTSVEMAL
                     FEMLKDASNDAFKKIAKIIK"
     gene            9145..9357
                     /locus_tag="PKX09_02965"
     CDS             9145..9357
                     /locus_tag="PKX09_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16818.1"
                     /translation="MKNLVLISGVLGVLTFLMGVITQLVGHSILFCAASWNSLTQTML
                     LFAIAFAVWEHEKCKCKQEEKEKEKE"
     gene            complement(9443..9832)
                     /locus_tag="PKX09_02970"
     CDS             complement(9443..9832)
                     /locus_tag="PKX09_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOU16819.1"
                     /translation="MKEIILKTNRRIELIDITAQVAEAVRSSGIREGICVVYCPHTTA
                     GLTINEHADPNVASDIVNCLSKLVPHNENYAHIEGNSDAHIKSTLTGNSVQLIVNNGN
                     LLLGVWQGIFFCEYDGPRNRRVWVQIK"
     gene            complement(9847..11118)
                     /locus_tag="PKX09_02975"
     CDS             complement(9847..11118)
                     /locus_tag="PKX09_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HOU16820.1"
                     /translation="MKRNRPLIVLIGLILILANLSFAQKILIPMDQTQSDHLKAYGIV
                     YWSLKQGVNVEWLLNYRGGSFLLDNYPGIVKELRLRGVAYEEVSAAQNLQIYATIEEN
                     NMDVVLLEKAAKIAVYSPPNKQPWDDAVTLVLTYAEIDYDVLWDEEVLTGKLPEYDWL
                     HLHHEDFTGQYGKFYASYHNQAWYQNEVAENEAIAAKLGFRKVSEEKKAVARTIKKYV
                     NEGGFLFAMCSATDALDIALAAENVDIVAEIYDGDPADPRANEKLDYSKCLAFENFKL
                     YLDPMLYEYSDIDYPPSFQAVAPGAEADYFTLFEFSAKWDPVPTMLTQNHVGVIKGFM
                     GQTTGFRRDRIKKSVVVMGEVVNGEQVRYIHGNHGKGTFTFLAGHDPEDYQHFVGDPP
                     TRLELHKNSPGYRLILNNVLFPSARKKERKT"
     gene            complement(11124..13064)
                     /locus_tag="PKX09_02980"
     CDS             complement(11124..13064)
                     /locus_tag="PKX09_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU16821.1"
                     /translation="MKLGRRWGLLILLIFSLTAFVAAQPVKVVSPEEKLMNLRFNQAQ
                     RLEREGQLTQAAEIYRALVDAQPANYLYYSNYVNLLFRQKNLPELERVITRFMQLNPL
                     HENAAIDLGKLFYVRGDSAEAFQEWQKAVVKFNHGVSFYRALFNEMVGLRLYDEAERL
                     IAAARAHYRQPDLLTMELANLQIARGKYSSAARELVLYARANPRYYNMIAGQILRFPT
                     DSTLFIQIDSLLKAELLLLPDQAALHRLRADFLFKNGHYNEALEEIFQVEALTGNHGD
                     QALAMAKNLVQIRRYELAQDFYTKILAKNELQGIAPQALLGLAESFEKAGLEQTTAEP
                     LHYFYPGNFFFNTDFVQQINQEQSSLQKAFAIYDSLVANQPNSIYSAQALYRLADLRF
                     RAVRDFDGALNLLRQAQNITRDRQLILNCALRIGEVQIARGDPDAAIKYYQAEIVRWE
                     GTPGEKDLRVHLALAQFLAQEFDSLDIELKNLIPLLGPKHALFNDVVGFDGFFRANYS
                     ETDAAGKKAFAEFATAELLLHQNKLSEAEKIYAFILQNYPQTPIISAARFRLTQILLQ
                     FNRYAEAEATAEPFFTSENENADQIAFMLAEVADYREANNQAAAYYYELILEKYPNSF
                     LIDNVRKRLRELQRLSGVKKEI"
     gene            complement(13061..16453)
                     /locus_tag="PKX09_02985"
     CDS             complement(13061..16453)
                     /locus_tag="PKX09_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16822.1"
                     /translation="MRNNQSSYNRIERKLNDLRRKFARHTLQVGLLKVLLLIIIVTFL
                     IVVVEGFRYFKGSFRSDILFFTVGFSLIFLAIPIVYYFQIIRNRIRAYDDYHLARRIG
                     ADQPEIKDDLLNALQLRELLNKETQGYSSELIEQALERIARKIEPLNFDRIIPSQEVR
                     KSLRNLAIVIFCLVICIASSPAYFKNSMERLLHPGSEYPVKLPFSIESTRASSGVLGG
                     DSVTISFQCQGRFPHYLTLDLQYPDYTRQEILMVDDSGRSQYLIENLRHDLVYSAYYQ
                     NHSPFKPWRRIGTPPDTIRVINRPEILYVKSRFIYPEYTGLPAQTQETKSAEFNLLSG
                     TKISLEIRINKEIKEGHLSFASGNVLNLATNGDRARGEFIVKNDDQFEIIVADEKQVT
                     NINPIKYRLSVIPDAYPTITMLSPKNDLDLNADMVIPLGIRISDDFGFTRAAIRYRVI
                     KKYADYQEEETEITFPLAKLNITWQEVFFQWQIGELSLGPEDAVEFRVDIYDNDNISG
                     PKKASTDWLIARFPSLNELFSSVNEQQDEIYSSSEEVLKSLETTKKVLDEISRELLKK
                     PELKWEQKKQLEEEMKKTREAGEKITKLSEQLDDLIEKSRENQLFDAETLEKLAQVQE
                     AFQDIMTPEMREALDKLQAAIEKMDPKEVQKALNNFKTSQEQFSQELDRLLKIFRRIQ
                     IEQNIDELVRRFADLSERQRQVANELGKTAPNDQQKLNELAQAENAIKNDTEIAQNIM
                     KRTAADMAEFPMMPNKELQQLIDEMQALNLPQEQSQASSAMRKGDKSQAEKSAQNSQQ
                     NLQKLLERMEEFRTEFQRRNMADVMTDFRRILNKTMQLSYRQEELTEEIKNTPRQSDQ
                     IMDIAVKQSETRQNLSRLISDLIDLSGKTFGVSPRIGKNLGQSANSMNAAIAYMEERD
                     PGLAALSAENATASLNMTALELMAAMQELQKSGSASGFENYLQQLQELAGMQQGLNEE
                     TQMLGLGSPGDQGTLQQLAARQQQIRQSLEKLRDEIQGQGSTKQSGDLGGIAKDMDEV
                     IKDLENNQVLRRTLERQQRILSLLLDAQKSLRTQDFKEERQSKTGQEIVRESPDGLPS
                     NLGERRTLLQENLEKALRDGYSKEYENLIRRYFEELSKESVE"
     gene            complement(16456..17127)
                     /locus_tag="PKX09_02990"
     CDS             complement(16456..17127)
                     /locus_tag="PKX09_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOU16823.1"
                     /translation="MKKISWTFIVILLAVLNLAGQSKNLYGKFTIARLKYDGGGDWYS
                     NPSSIPNLLKFLRENLNMDVASDEVRVGIMDKDFFAYPYLYMNGHGNVRFSDEEVLRL
                     RQHLQNGGFLHADDNYGMDQSFRREIKRVFPDNDLVELPFDHPIYNILYKFPHGLPKI
                     HEHDGKPPQGLGIFYKGRLVVFYTYECDLGDGWEDPEVHNDPEEKHQAALQMGANIVL
                     YSLMQ"
     gene            17373..19013
                     /locus_tag="PKX09_02995"
     CDS             17373..19013
                     /locus_tag="PKX09_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU16824.1"
                     /translation="MTLFEFKTGIQTFGFDGQPLLFDSPGISFNGYKTSWERAMNSLE
                     LDLLKFNWLVYILPQNLLDFQTGIGAKYTYSLSEQKLPETWPQRLPGSVDQLFLSPRI
                     YEFNLNQTVIYQWAPNFYNYLTISYGQASGSAYKTHSNDYFLHQKGHTYSFALGFKFL
                     GSINYKLKEGYGVEIRYTVGDFGDLEDPHNISPIKKINFNTVGISLTFNSNLGGGRST
                     GDEAQALYQANDFIAAKATFEQFIADNPHHPSRFKARRMIKECDKRIPYQEVTLAELF
                     IARQNYSIATEYLARAQVTKDKTLLARINSNYERMIKQFVDTMNVMISNNRIDEAEQF
                     LNTIAKLNLPNTNELIYRYHSEIYFHRGAVFTESGYWEKAIDYFDLAIRYYPPIRQRV
                     EPYLVQIADGYINDANLSVDKKSIALALESLKQATALRPDIHYLTAPHIKNLEAGIEY
                     LKQQAAKQKAQESIGKTFNPPPDAPKPDIGMSTDQIRSSLGQPGSQTRLETGGGRIYE
                     LWIYLYPDGKELQIYFDNAKVVKMEALSPIDVISEMDE"
     gene            19014..19508
                     /gene="rnhA"
                     /locus_tag="PKX09_03000"
     CDS             19014..19508
                     /gene="rnhA"
                     /locus_tag="PKX09_03000"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOU16825.1"
                     /translation="MKIIYSDGACSGNPGHGGWATLIFNENQLLKVLAGYQENTTNNR
                     MEIIAALNGLKQLQPGEKAIIYTDSQYLRNGITTWLPNWKKRNWKTADKKSVKNQELW
                     LELDRLNHPGITWQYVRGHSDNLYNEICDCLARKMIASQGKVSDLNAEFEKYYWQLQK
                     KKKQ"
     gene            complement(19516..21744)
                     /locus_tag="PKX09_03005"
     CDS             complement(19516..21744)
                     /locus_tag="PKX09_03005"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOU16826.1"
                     /translation="MKSLEDLNPEQCRAVEYNAGPTLIFAGAGSGKTKVLAHKVAYLI
                     ENKIVAPQNILAVTFTNKAARELGERVEKYIGKKARQVNIGTFHSICARLLRREIECL
                     GYSSNFTIYDAEDQLSLLKKIINDRSIILESTSPKNVLSRISYLKNHMIEPENFSARP
                     GFMADRFVAVLYPLYQGALRKAMAVDFDDLLILPLKIFQLKPEVLEKYRALYQYILVD
                     EYQDTNRPQFLLIEALGKKHQHVCVVGDDDQSIYSWRGADIRNILSFNEIFPQCRVFK
                     LEQNYRSTNNILKAASAVVSHNKQRATKTLWSQKSDGDLLVKLDAETEYDEAALIASW
                     IQREILQNKRNFKDFAILYRTNAQTRVLEEILRRNNIVYVIVGGVRFYERKEIKDVLA
                     FFNVISNPHDDISLKRIINLPPRGISKTTVQHLGELALAKDISLFDAMSYADELNLAT
                     RAIESIKKFYDQINDLRSLRNTLSFEEWARVVIVSIGLRNYYKESEGEEATERLANLD
                     ELLNDISEFCQTNEKPSLESYLENVTLATSIDEWEDTKNAVTLMTLHGAKGLEFPVVF
                     ICGLNQGLLPLERDLTHEALEEERRLFYVGLTRAKEKVFLSSSRQRMRAGEVAYSSES
                     MFLNEIPPELITICDFREGIISSKRQPRTSNRSTARKIKDTFERVESNPIKIAGINSS
                     SSEKTSPTSRTIEPGTHVSHKIFGTGQVVNIDGVGQNARVTVNFRSCGRKTIVARFLN
                     IN"
     gene            complement(21741..22802)
                     /gene="mltG"
                     /locus_tag="PKX09_03010"
     CDS             complement(21741..22802)
                     /gene="mltG"
                     /locus_tag="PKX09_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOU16827.1"
                     /translation="MKRIKSNFKPSVKRPLVYIPLIIFFIVILYLAILVTPLSSSNTN
                     QVITIQPGASVQEVSELLYEKGIIKYPKRFILATKMMGKERKLCAGRFDLYGAINYYE
                     LIKTLSDLESVTVRVTIPEGLRAREIARLLENQIGISADDFMQKVMDSRFTHQLGVEA
                     NNLEGYLFPDSYHFIVGDSPENVISRMVERFWEVVNDSVQAEIRRTGYTLHQIITLAS
                     IVEGECQVDFERPLVASLYYNRLRRRIRLEACPTIQYIIPDSPRRILRQDLKIDSPYN
                     TYLHYGLPPGPVCNPGFKSIKAAIYPAKTKYLYMVSNGDSTYTHTFTANYEEFLAAKR
                     KLQKIRTQDKLVASEDYIN"
     gene            complement(22815..23864)
                     /locus_tag="PKX09_03015"
     CDS             complement(22815..23864)
                     /locus_tag="PKX09_03015"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOU16828.1"
                     /translation="MLDITTKENIILYLSQWIEIVRIEDRDKYLVIEGRQRDTTSLRK
                     IARNSRNLISSIKKISTGSIDRFIVKPEKIEKRIPTVNLVLFLITILTTLLAGAIMEG
                     ANPFVHPLDLLKGVPFSVTLMVILGFHETGHFLMARKHNVDATLPYFIPAPTIIGTFG
                     AFIKMRSPVTRRQALLEIGAAGPICGFLVATPALFIGLKLSEVVSLSEAIGIQLGDSL
                     LMKIASALIFPNLEVGQDILLHPVGFAAWIGMLVTMLNLLPIGQLDGGHIAYALLGDK
                     YPYVAWGALIATLALSFVSLNWLVWGLLVFFLVRVKHPPIYDDNSPVTLREKLIGLAS
                     LVIFILTFIPVPFKQ"
     gene            complement(24176..26860)
                     /locus_tag="PKX09_03020"
     CDS             complement(24176..26860)
                     /locus_tag="PKX09_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU16829.1"
                     /translation="MTVTMAQELHTPAPQPISGTPFLWNKYPEIVFHHRLAKPVAGDT
                     TFFIRKNVEDNSAEFIEIAFLRLINTAEIEIYAEKTEFDAGRITQSDAERVRDIMLYD
                     TPPGSINPAKGIYYNEIDIFGELPDKDGNGKLFVLLIDVRDNYEVGKSDTYVAGYFDP
                     LDQVPGKGNYGEIIYIDTNPADAKDLGTLSIVAHELQHLIHNFYDSDEDVWVTEGLSE
                     LAPRLLGLPARSFASFLNETNRSLMDFDGSIADYAKVGLWTYYIYQRFGLDVIKKIFN
                     NKNDSFNGFIGGLSSAGYDGSVAELLRDWFVANLLNDPTMGDGQYGYGGAAISPISSN
                     YFHSNFTEGEQISLALNPAAAVYIQFYSGQNIEFVMNYQSSYSFGLAVIKHYETPEIT
                     FVTCNGTGTYNFSDETFGSDYAKLTIIPYWPAEIAATVQANISYSATGIGGYAESELG
                     YDNDMLDYFIRLEDAEAAEKFDLNLSEATLSAIKILTYDSSPVTIKIYSNLAEAPLLT
                     MSGIIPDEQAWTKIILGNPIDLADLSSFAVSVTSSGNSLGYSSAQDGFGHAYIKTDEI
                     FHDLCNYTVTGGSEDVSLTGNWAIRAIVSGKIVIPPRIILTPDSLWFWHDEYTQTFRI
                     SNSGTEALEWEISGELPNWVKIEPLFGTVNIGSAEIAVTIDRNLLTSGFHDINIPIIS
                     NGGDELLYVSVLKRNTSTAQSALIPTEMVFSDDISRTTLPIFNIGIGSSQFAFWSEEA
                     CLAFLPSSGFIPEDDTIDVQVFLDRSAASDSILAFFFFDGVDTLAYECIYSDWQGETA
                     THFTILNPFPNPFLPGENPLIYFPFRLTVEKPVTALIFNLNGQEVYSEKINNPKIGLN
                     VWTWDGHNQKGNPVASGVYFIVFRQGERRARQKLVLLR"
     gene            complement(26902..27912)
                     /locus_tag="PKX09_03025"
     CDS             complement(26902..27912)
                     /locus_tag="PKX09_03025"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOU16830.1"
                     /translation="MSIAVLGAGSWGTTIAIHLYNKKLPVKLWEYFPENVAYMNKTRR
                     NPLLEGIPIPRAIPILNDLAEAVKNANFLVLAVPSQSMRSLLEKIKGQIEPDTIIVNL
                     TKGIEQNTLKRMSELITEILNHPPEKIVTLHGPSHAEEVARQVPTAVVAASINEETAQ
                     LVQEVFHSPYFRVYTNTDIIGVEIGGAVKNVIAIAAGICDGMRLGDNAKAALMTRGIM
                     EIVRLGLRVGGREVTFAGLSGIGDLIVTCNSQHSRNRYVGEEIGKGRKLDDILNGMSM
                     VAEGVTTCLTVHQMAEKYKVVMPISEEIYKVLFKNKDPQTALWDLMNRDPKQERHSLS
                     KY"
     gene            complement(27947..28939)
                     /gene="purM"
                     /locus_tag="PKX09_03030"
     CDS             complement(27947..28939)
                     /gene="purM"
                     /locus_tag="PKX09_03030"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOU16831.1"
                     /translation="MSVSYKDAGVDIAAGEQAVKEIKNLVKQTFTPNVLTEIGLFGGC
                     FAFPVKNYRQPVLVASTDGVGTKLIVANLLDRHNTVGQCLVNHCVNDILTTGARPLFF
                     LDYIGTGQLKPAITTEIIRGISLACKANGCALIGGEMAEMTDIYQTKDYDLVGTIIGV
                     VEREQMLIGRVGKGDVLLGLPSTGLHTNGYSLARKVLLTKYSVTDFIPELNATIGDAL
                     LAIHRSYLNVIEPLLADPDLHGLSHITGGGIIGNTSRIVPENLTLKINWQAWEWPPLF
                     KFIQKTGEIDIEEMRRVFNLGIGMIIICAASAVDKFSQVLQKSGEKAVILGEVC"
     gene            complement(28940..30751)
                     /locus_tag="PKX09_03035"
     CDS             complement(28940..30751)
                     /locus_tag="PKX09_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOU16832.1"
                     /translation="MSDFSYSTISEMFVGITDKYTDRPMYKYKKDGKWLGLTFSEVRN
                     RVEQISGGLKALGMKAGEHVAIIAANNPLWAMTDYGVICAKGVVVTIYPTLTAKQVWW
                     IARHSDSRFIFCGDREQTEKVIPLLNELPEVYKVIIMDDTKIDHPKAIMMSELMQMGA
                     EYRGQNPSAFLEDIHNTKEDDLLTIIYTSGTTGEPKGAMLTHGNLTSNITGALQIVEA
                     NENDTFLSFLPLSHGFERMAGHFLATGSGACISYAESIDTVAENMLEVHPTLMVAVPR
                     FYEKVYARVIDSISTSSKISQKIFWWAINVGKKAVFLKSENRNTGAILDNQLKIAQKL
                     VFSKLYEKIGGNLRFFVSGGAPLSKEIAQFFQAAGIPILEGYGLTETSPVISVNPLSK
                     CKVGTVGPKLPNIEVKIASDGEILTRGPHVMLGYYKDEASTRDVLDADGWFHTGDIGM
                     IDGDGYITITDRKKNILVTSGGKNVAPQPVENALVTSKWIEQIVVIGDRRKFVSALVV
                     PAFVNLEDWAKENNLQFHSREELIALPAVKELYDRVIAESMEGFAQFEKIKKYVLLPS
                     EFTIESGELTPSLKVKRSVVEKHYADLIESMYQEAES"
     gene            30820..30903
                     /locus_tag="PKX09_03040"
     tRNA            30820..30903
                     /locus_tag="PKX09_03040"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30854..30856,aa:Leu,seq:caa)
     gene            complement(30997..32370)
                     /locus_tag="PKX09_03045"
     CDS             complement(30997..32370)
                     /locus_tag="PKX09_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU16833.1"
                     /translation="MKKYLFIILIAFTTTLALPACSGGKETKKVERRTEQTKVTEKTE
                     PKKVQSPKTSTETTNLETVRKDTAGMVMQSVTAVKESQLDSVTPAKKDVQNEPDSTPL
                     TGNTSELAIQPSPVITDEIKTDADLLPTSNETNGVLESKVVTEKPGLCTKEAETSAKV
                     LKILIFDDIFFDADETETPNLTFNSNYIVTLNKVVKALKSDPDIKIRLKGYAMDTGTK
                     SGNAIIKSQKRAITVGKLILDLFPEEEKDSVANRIEIVPVGADEMLIESDNQVKELLN
                     HRVSIELFVGEVVNPTIADFVFHGKAPEPEVTSTAPKPVQREKAKTPKSRPRTRDMLY
                     ETGNDLFQQKKYEDAISVYTEFINLDPQHPLADNAQWWIGECYYFLGDYVSALNAYRK
                     VFGLGDGNKSAYAQLRMGYCYNRMNQVDLAVSAWEKVIRDYPNAIEEVTKAKKAIQIY
                     SGDVNQD"
     gene            complement(32450..32953)
                     /locus_tag="PKX09_03050"
     CDS             complement(32450..32953)
                     /locus_tag="PKX09_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16834.1"
                     /translation="MKELIKTIALVLVVVAVVYFFFEFEKVNKRLDILNARDSTHSVT
                     INDFDHNLHDLSLEFVGRGKHIQQFQQSLKDMNSRLDSVDLALSDKIEETNTSIAELK
                     IDIDSRFNVMKTDQDGINERLNKFQRETQRTLTDLQSAVTRMSRELSDLDKRVKTLET
                     PPTPAKK"
     gene            complement(32989..33306)
                     /locus_tag="PKX09_03055"
     CDS             complement(32989..33306)
                     /locus_tag="PKX09_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16835.1"
                     /translation="MEDKKVDFSIEESRKNFLITKFDDLFQGINNLYGKELMEELFHR
                     MEKTIAIFHQDVENLIKELKSSNSTGSTDQPIMGISDIPQTPAEPNPFSANEDNDWAK
                     HLG"
     gene            complement(33389..33574)
                     /locus_tag="PKX09_03060"
     CDS             complement(33389..33574)
                     /locus_tag="PKX09_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16836.1"
                     /translation="MLTRMTIELERSELLEFRQVLQQLTSNQPSVEIKLNSVIRGVCR
                     QIIREIDRWEDLKQERW"
     gene            complement(33610..34665)
                     /gene="amrS"
                     /locus_tag="PKX09_03065"
     CDS             complement(33610..34665)
                     /gene="amrS"
                     /locus_tag="PKX09_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOU16837.1"
                     /translation="MNVNCVVFNVEKLELPKNLYPARWWHALENNEVECFLCPRHCRL
                     KNGKRGVCRVRTNIEGRLFSEVYGKPIAIHIDPIEKKPLYHFQPGSRIFSIGTAGCNL
                     QCEFCQNWDISTADGHLGMGRIVQPAEIVQSAIDNQCGSIAFTYNEPTIFGEYVVDIA
                     KIAHQSNLKTVKVTNGYITQEAIADIYPLIDAANIDLKSMNADFYRKRCHAELTPVLE
                     AIVAIQKVGTFIELTTLLIPGLNDSEGEIQKLCDWILDNLGNDTPLHFSAFHPAYKMS
                     NWPQTTKEVLDRARTIALRTGLHYVYEGNVLTDSESDTFCPACGRLLIKRGWYTQVTK
                     SLQGNRCECGQKIPIIW"
     gene            34795..35067
                     /locus_tag="PKX09_03070"
     CDS             34795..35067
                     /locus_tag="PKX09_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOU16838.1"
                     /translation="MAQVKTTKKVKTKEKSASFIKALSFTKINYLLFAIGIAVIIIGY
                     ICMATGETYSFRSLTVAPIILLIGYLIIIPLAILYRKKEEKTPSES"
     gene            complement(35118..35408)
                     /locus_tag="PKX09_03075"
     CDS             complement(35118..35408)
                     /locus_tag="PKX09_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015274273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOU16839.1"
                     /translation="MPTYEYKCEKCGYEFEQFQKMSAEPLKVCPKCGQKSVTRKISGG
                     AGLIFKGSGFYVNDYGDKNKTKSPSPTTPEDKPTSTPKAKEKTITTSSNSSN"
     gene            complement(35429..36406)
                     /gene="pta"
                     /locus_tag="PKX09_03080"
     CDS             complement(35429..36406)
                     /gene="pta"
                     /locus_tag="PKX09_03080"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOU16840.1"
                     /translation="MKLIEEIRSQAQKCKKTVVLPEAHDERILKAAEILEKEGLAQII
                     LVGDPEIIGQHTREINVNLGSIGIFDPVQFEKLDDFINTYYEKRKHKGMTLENARTVM
                     LNPTFFGAMLVEKGLADGCVSGADTTTGDVLKAALHIIGTNPDTPTVSSDILIITPDE
                     ERIWSFADCAVLPNPTAEQLADIAIATANTHRRVVKTEPIVAMLSFSTKGSAKHELVD
                     KVIQATAIVRGKYPDLRIDGELQADAAIVPTIGAKKAPGSPVAGQANVLIFPDLNAGN
                     IGYKLVQRIGNCEAIGPIIQGLKKPMNDLSRGCSVEDVVNVTAIICNLV"
     gene            complement(36408..36887)
                     /gene="coaD"
                     /locus_tag="PKX09_03085"
     CDS             complement(36408..36887)
                     /gene="coaD"
                     /locus_tag="PKX09_03085"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOU16841.1"
                     /translation="MKIAVYPGSFDPITNGHLDILKRASSLFDLVYIAVARNLQKQPI
                     FTTEERLALIRGCTSEFENVKMDSFDGLVVEYARQVGAEVIIRGLRALSDFDYEFQMA
                     LMNRHLDENINTVFLMPHEDYTYLSSSMVRDLAKFGAEIGSFVPANVKAALLKKFRE"
     gene            complement(36888..37412)
                     /gene="rsmD"
                     /locus_tag="PKX09_03090"
     CDS             complement(36888..37412)
                     /gene="rsmD"
                     /locus_tag="PKX09_03090"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOU16842.1"
                     /translation="MVRVIAGLYKNQSIMSDKKATIRPTQDRVKAALFSILGDVSGLK
                     VADLFAGTGNLGIEALSRGAERCTFVDNNWQSLNLVKHNLAKLKLEQRAEVIQADVLK
                     YLAQKPLFDLIFADPPYEYSSYNELLMLFGQLRPDTQIVLETDRRFDLPSSFKPREIK
                     IRRSGDTNLNFIRI"
     gene            complement(37415..38599)
                     /locus_tag="PKX09_03095"
     CDS             complement(37415..38599)
                     /locus_tag="PKX09_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16843.1"
                     /translation="MIKINLAATPGLQQEPKDIAISQSLKDILIKMAVEPKNQPNDDQ
                     AISKSSTKPIPENIEKKLDAFASQGSNSSMPGETDAKKSISQPSPKVSDKLREAEQIV
                     ERKEEEYSRHLSNRLILNIGIWILVLVIVGALGYWGYTKYFNPRKSAITAKPTGKSDL
                     PVEQPILLTEQSQPSSTSDMPEVDQPVLIPINQIDEIYLPPVLTGISRCQILSQLISV
                     LPDNSRIQFLKLDKDVVNFLIFVDTENEAQQVKDEVQKLPGIGSIQAFYIEHVADTPS
                     PAVQMMAILNLKTESAVARNIRFYDDVSLSQVLWAIGKRQNLKLQPLNIAEPPAPKPR
                     LAEIRGNGSLNSIINLLSELSNFDLNLSVEKIAINLSADSAVDTGILDFSINSVIYPQ
                     KL"
     gene            complement(38608..39552)
                     /locus_tag="PKX09_03100"
     CDS             complement(38608..39552)
                     /locus_tag="PKX09_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16844.1"
                     /translation="MLAISLSDNFIRYGQLIKTDNSISIELIGKKKLPFKFDTTSLKN
                     PDLIPSIESLLLNIKNRLQVQERFLALSAPSQLFDITINSIDLGLEKQKIAEILDWNE
                     KQRLGVVFNKKFVQHYPLKQRISENKRDYLTISYFKELGRAIYQACQRLGFIIKVFDL
                     NIFSAANALESLYKEKNGKKWGLWLIGEKYHILLLIKSGEISQYLEFDLNNDGDYNIL
                     VDSNPDEDGKEIVSQINNLRNLSSETIQDIENLYFFTYDVNSEFYNMLLTYDIDNLRC
                     VDPFENFKPVKIYQDDGSGPGAMSQFLDVMGLLYRFML"
     gene            complement(39577..40359)
                     /locus_tag="PKX09_03105"
     CDS             complement(39577..40359)
                     /locus_tag="PKX09_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16845.1"
                     /translation="MSDETAKFNQEEEIIPEYKPTLVKVLNVVAFILIIVLIAVIYIP
                     SSIWKEENRIRELGRQRMQIQYDVQKYYNQMAGTHQPDPVLAMKVVSAVRDSTRADSN
                     FYGQKVIKLPDGKFNLEVPKNFYASFDTNFAFKYELSDTVIDTTIKITKWNPELMTID
                     TLYVISSMLEEVKTDPNFRSVIDTELTQRVANNTYYRRYYLTEDLAMRPLLDTPYTIL
                     KTEKGIRVEDPLKTVIREPRYLFFTFVDSSHGYIENGEPSWK"
     gene            complement(40352..40789)
                     /locus_tag="PKX09_03110"
     CDS             complement(40352..40789)
                     /locus_tag="PKX09_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOU16846.1"
                     /translation="MINLTRQEKMVIQLLVACFLVGATIRIYKMRSNRINNEKSRQQT
                     VVTDSFQLYASQIDSEYTELQRSGHSTYQSQKSTALQQKINLNTATKQELMLLPKIGS
                     VTADHILEYRAQVGKFNRIEELLNVKGIGTKTFEKIKGEVTLE"
     gene            complement(40846..42000)
                     /gene="dnaJ"
                     /locus_tag="PKX09_03115"
     CDS             complement(40846..42000)
                     /gene="dnaJ"
                     /locus_tag="PKX09_03115"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOU16847.1"
                     /translation="MAKDYYEILGVNRNAQADELKKAYRKIAMKYHPDRNPGNKEAEE
                     KFKEAAEAYAVLSDPEKRRVYDQYGEDGLKGTTGFSGAQSMNMEDIFSMFGDIFGGHF
                     GGFGGFDDLFNGGGRQRSTRTRTRTTQQRGSDLKITMPLTLEEINTGVDKKVKIKRQE
                     ICPNCNGTGAKPGSSNVTCPVCQGSGEVREVSRSFFGQMVNVRPCSNCQGEGVIVEQR
                     CPQCGGEGRIQSVKELTIKVPPGVTTGNYLTMHGEGNAGVRKSSRGDLIVIFEEKPHE
                     YFTREGDDIYLDLHIMPSEAVLGTEIVVPTLSGKVSLTIPPGTQPSKILRLKNKGLPH
                     LQHPGRGDMMVRIQVKIPERVSGKELELYRELFKFESNKIKDEKRYSKIR"
     gene            complement(42002..42556)
                     /gene="grpE"
                     /locus_tag="PKX09_03120"
     CDS             complement(42002..42556)
                     /gene="grpE"
                     /locus_tag="PKX09_03120"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOU16848.1"
                     /translation="MEDISINEKTSGINKNQTRSTKKQTSEKERLSAKIAELEAKVAE
                     YEKALNEANEKFLRLAAEFDNYRKRREKEYSDIIEYAGEEVLRNILPIMDDLERALNN
                     INDDQSLREGLELIYRKFQKTLQNLGVEPIESVNKPFDPNLHHAIMTREEAGVAPDIV
                     LEEYEKGYKFKNRVLRYAKVVVSK"
     gene            complement(42857..43723)
                     /locus_tag="PKX09_03125"
     CDS             complement(42857..43723)
                     /locus_tag="PKX09_03125"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOU16849.1"
                     /translation="MTILWFLIKEGFRGLGRSGYAGAFSIIIIALSLILIGFGYVAAR
                     DTLVMVENIRSQFDITIFVHRNASLTDLENFIEELSYFKEIEKIDYISTDSAAAQFKA
                     EFGEDIFSILDYNPLPPSFTLRLKPQARNLASIEDLTTRIKQYPIVDEVRYRENFLRL
                     LEKYQRLIFIAVVTAFIGLTLISIILASNSIKMTIFSRRNVIATMKLVGATNNFVRAP
                     FLIEGTLEGLIGALLASGVIGLISYFFNNYLQGLTGYQIIVSYRYYIGLLVLGSLIGL
                     IGSMRAIRRFLE"
     gene            complement(43720..44379)
                     /gene="ftsE"
                     /locus_tag="PKX09_03130"
     CDS             complement(43720..44379)
                     /gene="ftsE"
                     /locus_tag="PKX09_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HOU16850.1"
                     /translation="MITFFNVSCTFGKDAGVKDVNLTINEGEFVFLTGSSGAGKSTLL
                     RLIYMDLFPEKGKVVIRNFDSSKVRPNEIPFLRRKVGMIFQDFRLLEDRNVYENVALS
                     LYVSHYKTREIKNRVYNVLNQVGLGHRVYYNPRELSGGEQQRVAIARALVKEPVVLLA
                     DEPTGNLDPRVASDLMNLLIKINQTGTAVIVATHNYSLIPRVANARIIQVNEGEIINP
                     V"
     gene            44552..45925
                     /locus_tag="PKX09_03135"
     CDS             44552..45925
                     /locus_tag="PKX09_03135"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HOU16851.1"
                     /translation="MNKLRYWIITLILDVWCFSATGPCFDDYFLDQTMRLDFYFTGTK
                     TQNIFSLDAVYISPLWSGSQTNLIDTLNLGHHLIRVYDVDSQTLIYSRGFSSIFNEWQ
                     STNEAIQEIWRTFHFSVLIPLPRQAITVTLSSRDRQNQFIEYFKTNIEPDSRFINRDG
                     VHTDYRLKKLIYNGAPTQKIDLLILPEGYTKSEMRQFRQKCRHFTEVLFNVSPFKEES
                     DKFNVWFLEVPSAESGIDNPRGGTFKRTAFDLTYNALDLDRYVLTLNNKTVRDIAAQA
                     PYDFIIFIFNSDKYGGGGIFNLYSTCYSTAAKPEQKGWPEYVFVHEFGHSFAGLADEY
                     YTSSTPYNELYPMGVEPWEPNITALLNPTTLKWLDLVSPEVPVPTPWEKSAYEQATYS
                     NASEFEQFLKSQKYYGKIGAFQGAGYASEGLYRPCLDCIMFSRNEIAFCPVCQAAIRK
                     IIHFYSE"
     gene            45999..46976
                     /locus_tag="PKX09_03140"
     CDS             45999..46976
                     /locus_tag="PKX09_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU16852.1"
                     /translation="MPNLNSIEIKKRAIELYKLIDPCQICPRLCRAKRLHGAAGFCQV
                     LDKAILSSALAHFGEEPPITGRGGSGTIFLSSCNAACIFCQNYQISHERIGNPVSISQ
                     LANEYLHLQAVGCENINWVTPTPHLPFLVDALASAVEQGLKIPVVYNTNGYDRVEVLR
                     LLDGIVDIYLPDMKYGEDVWAEEYSGLKDYFTINVAAVREMYRQVGPLIINENGVAVK
                     GLLVRHLVLPYGRAGSARLFRALAKIDPQVPVSLMAQYHPCYQAVGHPVLGRRIFRRE
                     YLKALEDFENAGLLNAFTQDYISLEHEDFFFPDFNDNERIFNCRDNRKK"
     gene            47178..47609
                     /locus_tag="PKX09_03145"
                     /pseudo
     CDS             47178..47609
                     /locus_tag="PKX09_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624240.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            47887..49971
                     /gene="fusA"
                     /locus_tag="PKX09_03150"
     CDS             47887..49971
                     /gene="fusA"
                     /locus_tag="PKX09_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOU16853.1"
                     /translation="MDNIEIQRIRNIGIVAHIDAGKTTTSERILFFTGMTHKIGEVHN
                     GQAVMDFMKQEQERGITITSAAITTEWKDHQINLIDTPGHIDFTVEVERSLRVLDGIV
                     MVFCAVGGVEPQSETVWHQADNYKVPRIAFINKMDRAGADYFQTINMMNEMLGANAVA
                     FQLPIGDEADFNGIIDLITMKAYSYRELDMFESEIPSNLLPKAQKLREQMIEKLADFD
                     ENLMEKFINGMAISESDIRNAARYAVLNLCVTPVFCGSAFKNKGIRLLLDAIVDYLPS
                     PVDRGIITGRDANNPEVIISRKPHISEPLTALAFKITNDAYVGQQTFVRVYSGELHSG
                     SYIFNSTKQKRERVGRVLRIRADERTEITSLKAGDIGALIGMKLTTTGDTLCNENDTI
                     LLENIRYPETVLDMKIEPLAIKERDRLGIALSRLALEDPSFKVRFADETEETIISGMG
                     ELHLEIIVDRLKNEHKLDVNVGKPAVAFRETITREAEHTYRYKKQTGGKGQFAQIAFR
                     LEPNPGKGIEFVDRVKGGNIPREFIPAVEKGFREIVEKGLLAGYPMVDIKFVLFDGAY
                     HPVDSSEMAFRMCTILGMKEIINKTAPQLLEPIMKIEINTPDEYMGDAIGDINRRRGK
                     IENMRRHRKGSQKLNGTVPLMEMFGYASALRTISSGRAAFSMEFLHYTPLPKSIEESI
                     IEERRKKKAERD"
     gene            49973..51091
                     /locus_tag="PKX09_03155"
     CDS             49973..51091
                     /locus_tag="PKX09_03155"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOU16854.1"
                     /translation="MLEKTIKKPRLSFSLILLLSIISFFSFGHSQVQTILWPELNGTA
                     LWDSVIANFKPIKTLGYDKSRDTMYAVIDLKNGGSLSCVYTGYTIKLDRTKDPSADAY
                     NKLIDCEHTWPQSMGADSEPQKSNLHHLYPTFTNVNSSRGNDPFAEIPDAKTDKWWYL
                     NKSQTIIPTSNIDKYSEKENDPPACFEPREDHKGNCARSMFYFYAMYSEAYFTKDPDM
                     TFWNFQKETLLAWNYYDTVDADEYARTWKIATYQDNKPNPFVLDSTLARRIWFYKPNT
                     LVREKPSLADDFKLHPCYPNPFNKATNLAFTLPEVENVTLSVFDLNGHLVNEQQLGRF
                     PSGHHIVAWNGGNLSSGVYIYCLQAGQRAHAGKLLIIK"
     gene            51424..51765
                     /locus_tag="PKX09_03160"
     CDS             51424..51765
                     /locus_tag="PKX09_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOU16855.1"
                     /translation="MNIYVGNLAPNVTEGDLRVAFEAFGQVASASVIKDKFTGESRGF
                     GFVEMPLMAEAQAAISNLNGKDLKGKTITVNEARPRTEGGRGGDRFGGHSGSGHFGGK
                     PRDNRRGGGRY"
     gene            51939..52646
                     /locus_tag="PKX09_03165"
     CDS             51939..52646
                     /locus_tag="PKX09_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16856.1"
                     /translation="MNNNTEIVDLKKEQISSLDRPRYKYSLMARAFFLLMDIVTGCQI
                     TLSKAKLIEILASIPYRAWEVRHYSRLTRVYRNYEKVTRSQRIVTWSRSAQDNEYWHL
                     LVIHEKMKTDGIKDAWYLFPLVPFVMVAVYRLFAKTLAFCNIRRAYLFNAEFEDHAEH
                     VYAQFVADHPEWETQPVESDLVNEYGKFSSWADVFRRIGLDERDHRNHSFLLCGKPEA
                     VVSYNGMPKDINFPTGR"
     gene            complement(52649..53854)
                     /locus_tag="PKX09_03170"
     CDS             complement(52649..53854)
                     /locus_tag="PKX09_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HOU16857.1"
                     /translation="MKKNCGLMVLLFCVPLMAQKFDKLALTPPMGWNSWNRFGCNVNE
                     QLIRETADAMVSSGMKAAGYQYVVIDDCWQGERDALGFIQPDPARFPSGMKALADYIH
                     SKGLKFGIYSDAGWKTCGRHPGSRGYEYQDALTYARWGVDYLKYDWCNTEGLNAKGAY
                     LTMRDALFAAGRPIVFSICEWGDNRPWEWGKDIGHLWRTTGDITAIFDGQEGHGTWNS
                     WGVLQILDMRENIRQYAGPDHWNDPDMLEVGNGMTIAEDRAHFSLWCIQAAPLIAGND
                     LRDMSSEVIDILTNKELIAIDQDPLGIQGFKYLVKDNWEVWAKPLDKGDLALCFLNRT
                     LEPVKVDLDWKKYPVKDDEFHYYYLFNENEYKIRDLWLKKEIGTTAKPFSTQIGAHDV
                     IVLRLSPVK"
     gene            complement(54139..55230)
                     /locus_tag="PKX09_03175"
     CDS             complement(54139..55230)
                     /locus_tag="PKX09_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU16858.1"
                     /translation="MKFKNEHPRRVLTARQRKRPEDIRGPFFRDQTAIIHSLAFRRLK
                     HKTQVFFSPENDHICTRIEHSLHVATISATICRALKLDIDLATAIGLGHDLGHAPFGH
                     AGEKWLENVFGGFVHELHSLRVVDKLENYGQGLNLTYAVRDGIVSHCGEVDEQFIEIS
                     PTLNDLDNLQFRPVQPSTWEACVVRLSDSIAYLGRDLEDAVAANLINKEDLPEKLRKG
                     IGQSNSEIINNLVFDVIDWSTKNGKIGFSPEKFALISEFKEFSREKIYDNPKLKYWRS
                     YSGFVIETLKNYLIDLYEKYQNDYERYANSRTPLDVKFGKYLKSLHERYQAENCPSEL
                     IVRDYIAGMTDAYALHCFQNILTMGTRGL"
     gene            complement(55349..55825)
                     /locus_tag="PKX09_03180"
     CDS             complement(55349..55825)
                     /locus_tag="PKX09_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOU16859.1"
                     /translation="MTIFSIRDVFDIAVKIEERGEKFYRETAKVISKAEVKELFEKLA
                     DEEVVHKKIFRQMAQKIGAVKLESAAREEFYDYLEAYTQNLIFSDANDETKIPAINDA
                     KTALLYAIERELDSVLYYKEIKELIPASEHQLISGIIDEERRHVVRLSELKKNLTF"
     gene            complement(55837..>56633)
                     /locus_tag="PKX09_03185"
     CDS             complement(55837..>56633)
                     /locus_tag="PKX09_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOU16860.1"
                     /translation="LSLGNKTLRFIFTPWVHWPETISTYLEEDKILFSCDFFGSHLAT
                     SNLFVDDESRVYRSAKRYYAEIMMPFRSNVRQNLEKVKNLAIAMIAPSHGPIYPNPEF
                     IMEAYADWTSDSVKNIVLIPYVSMHGSTEAMVQYLTKALIEREITVQPFNLSRTDIGE
                     LAMAVVDAATVVIGTPTVILGPHPAALSAAYLVNLLRPKTRYLTIIGSFGWGGKMPEQ
                     IKALLSGMKAEFLSPVIIKGYPKLEDFSALDRLADDIAARHRALSS"
     assembly_gap    56634..56733
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(56810..56974)
                     /locus_tag="PKX09_03190"
     CDS             complement(56810..56974)
                     /locus_tag="PKX09_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOU16861.1"
                     /translation="MTKYVCTVCGYVYDPRKGDPDSGVPRGTAFEDLPDDWVCPQCGA
                     EKEDFEPQED"
     gene            complement(57012..57587)
                     /locus_tag="PKX09_03195"
     CDS             complement(57012..57587)
                     /locus_tag="PKX09_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOU16862.1"
                     /translation="MPSLKGTRTEKNLLTAFAGESQARNRYNYFASQAKKEGYEQIAA
                     VFAETADNEKEHAKRFFKFLEGGEVSITAAFPAGVIGTTAENLLEAANGEKYENSVMY
                     PEFAKVADEEGFTEIANVFRAVIVAETQHEKNYRTLRKNVLENRVFEKEQVVTWRCRN
                     CGYIHVGTKAPEKCPACAHPRAYFELLAENY"
     gene            complement(57608..57811)
                     /locus_tag="PKX09_03200"
     CDS             complement(57608..57811)
                     /locus_tag="PKX09_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOU16863.1"
                     /translation="MKYTFKIPTMSCQHCKMRIEKALSPYVNPANIAFDLTSKKVVVE
                     TDTPLAILIEAIESVGYPVEHDN"
     gene            complement(57877..58101)
                     /locus_tag="PKX09_03205"
     CDS             complement(57877..58101)
                     /locus_tag="PKX09_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOU16864.1"
                     /translation="MKEKVAEVIESIRPMLMADGGNVELVEVTKDGVVKLRLTGACGG
                     CPMSTYTLRMGIERRLKEAIPEIKSVEQVF"
     gene            complement(58115..58519)
                     /locus_tag="PKX09_03210"
     CDS             complement(58115..58519)
                     /locus_tag="PKX09_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOU16865.1"
                     /translation="MINIKGELEKRDIKPTFQRLCVLELMEQSSEHLTADAIYESLLK
                     KMPTMSRTTIYNTLNLFLEKGLIAPIMITGTEVRYEISTNQHHHFLCLQCNRIYDVDV
                     QCPLFESKEVDGHKIQEIHGYFKGICRNCLNQ"
     gene            58657..>59249
                     /locus_tag="PKX09_03215"
     CDS             58657..>59249
                     /locus_tag="PKX09_03215"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HOU16866.1"
                     /translation="MSKIEEYLGKDADFLLNHKCMTISKELLHLPGPDFVDRILLYSD
                     RPVGVLRNLQTMYNTGRLSGTGYLSILPVDQGIEHSAGASFAPNPMYFDPENIVKLAL
                     EGGCNAVASTLGVLGSVARKYAHKIPFLLKLNHNELLSYPNMYDQTMFASIDQAFDMG
                     AVAVGATIYFGSAESRRQIMEVSEAFEYAHQLGMVTVL"
     assembly_gap    59250..59349
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <59350..59568
                     /locus_tag="PKX09_03220"
     CDS             <59350..59568
                     /locus_tag="PKX09_03220"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007902999.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     D-glyceraldehyde 3-phosphate from D-fructose
                     1,6-bisphosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HOU16867.1"
                     /translation="GCYMGRAGLINSGGASGEDDLIEAVKTAVINKRAGGMGLILGRK
                     AFQRPMAEGVKIVNAVQDVYLDKNVTIA"
     gene            59667..60143
                     /locus_tag="PKX09_03225"
     CDS             59667..60143
                     /locus_tag="PKX09_03225"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOU16868.1"
                     /translation="MTNQSSHPNWIIGLFLILLGLLIFLIQFDYFDWSDFWPLIIIGV
                     GLLFLVGFFVNRNNYGLLMPAMILLIVGTLFLYLERTTWTNMNYLWPIFILAPGVGFF
                     FMYFFGPQGNNLWIPGTILIVISVIFLAGEWTILRYWPVILILLGIYLIFSKKPSS"
     gene            60170..60850
                     /locus_tag="PKX09_03230"
     CDS             60170..60850
                     /locus_tag="PKX09_03230"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOU16869.1"
                     /translation="MPKDTPNPKSNWWQKWKTSQRNKIIAGIIALIPVVLTIYFIKLV
                     ISFFDRIVSPVLDPLIGFHLPGLGLIVALIVIYLLGLFITNFIGKSLILLIEKWLNYI
                     PIVRTIYNTSKQVMNALSFSKAGFEKTVIVEYPRKNTWAIGFLTGEVSDHNGKRFLSI
                     FLPTTPNPTSGWILLFPEDEVYSSDMNVEQGLKAIISAGAILPPKLNFRSTDKVNFSE
                     KNESQIKQ"
     gene            60936..62720
                     /locus_tag="PKX09_03235"
     CDS             60936..62720
                     /locus_tag="PKX09_03235"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOU16870.1"
                     /translation="MKIKYIDGKRFIRGIKVGAYKIVNHQDYLNKINVFPVPDADTGT
                     NMAATMKTIVDNIQEDTNQIDEATKNIANSALEGARGNSGVILAQFFYGLAKGIGKNK
                     RLTTIQFGEAVNSAKSHAYEALSKPREGTILSVIKDWAENIYENSKKIFDFQELLNHS
                     LEVARHSLEKTRTKIEAMRNANVVDSGAQGFVYILEGISNFINRGRLKELEKNCIQPV
                     SVIARTEVLPEQITYRFCTECLIEGKNIPIENLRLEFEPYGDSIVLAGSPQKARLHIH
                     TNEPAKVFKIAHQYGDLLQQKADDMLKQYLIAHNPHPKIALVVDSACDLPPEFIDRSF
                     IHIIPIRLTFGQSSYLDKVTITPEYFYEMLDTEPEHPRTSQPSPADFINLYSFLLSHY
                     DSIISIHLPATLSGTYQNALKAAQNFPKRKISVIDSKALSVSFGFLAELAVELITAGK
                     SHNEIVNELEKATHRTQLFIGVPSLKYLMRSGRVSKAKGLIAKLFNIKPILNLDEYGS
                     PQHISKSFSKKGAFTKVLQMASNFAAQKVNPRFAIAHANNLKIAEYFNNELKKRFKVE
                     KINILPVAPMLGAHAGNGAAAIAVTWSE"
     gene            62720..>63060
                     /locus_tag="PKX09_03240"
     CDS             62720..>63060
                     /locus_tag="PKX09_03240"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOU16871.1"
                     /translation="MITLLAIIVFSYLIGSLPTAIIVGKITKKIDIRQYGSCNAGATN
                     AFRVLGWRAGLFVALFDIAKGAFTTAYISQIRFDIPPLNPTWLMILAGLCAILGHTFT
                     IFAGFKGGKGVA"
BASE COUNT        18174 a        13789 c        12177 g        18720 t
ORIGIN      
        1 ctcaatccat aaagtatttt ttaaaaactg attcaggctt taccccctgc catttttcgg
       61 accaattcga ttaatttttc tgattcgctg gatttatcga aaaaataatc agttccttct
      121 tcgagacact ttttgcgata ctggggataa ggataatcgg taaagacaat tatacgcaaa
      181 tcgggacgat tttgacgcac cgttttaagc acataaatgc caccagcgcc cggcatacga
      241 atatcgagaa taactacatc cggcttttgg gcatcaattc cgctaatagc agattcggta
      301 tcctcaccat agccgacaat ttccacccct ggtatttcta ttagcagatt cgttaaatgc
      361 ttacgaagga tttcagaatc atcggcaata aaaactttca tgcttaaagt attttatccg
      421 aaggtgtgag gttcatgtaa ttcataattg agtaaaatat atattaattg caccgacttg
      481 taaatcagac aaaacctgat tttcttgtcg gtatttatcc tacaagatct gatttaaatt
      541 gataaacaaa atctggcaag tctataaaaa agaaaagccc ggctaagagc cggacttctc
      601 tcgattgagg gtgattggga ggaaattatt tgaccttaac ttcaatctgc ttggcgagtg
      661 attctttctt tttaggaatg acgatgttca gtacaccatc tttaaattcg gcttcaatct
      721 tgtctgcctc gaccgaatca tcgagtcgga aactccgttg gaatgaacca tagatgcgtt
      781 cagtgcgttg ataattctta tcctcgactt tagattcgcg ttttttctcg ccgctaatag
      841 tcaggtaatt atctttcact gcgattttga tatcatcttt gttcatacca ggcagttcaa
      901 cttggatgtt ataagcctta tcctcttcga tgatatcaac ggcagggctg aatgctaata
      961 aactggtttc ctcttcatac tgcggattga agattgattc gaacagacgg tcaaactcat
     1021 tccttacatc caaccacgag cttcttggag tccatctagt aattgccata acacacctcc
     1081 tttcttttta gattagtttg tttgtttatt ttatttcaat cgatttcggt ttgctttccg
     1141 gtgatttcgg aacaacgatg gtcaaaatac catctttgaa ctcagcttca attttctcat
     1201 ggtcaactaa ctccggcaag cggaaagaac gttcaaacga accgaatacg cgttcacatt
     1261 gatagaaatt ctttttgttg attttcttaa cccgtttctt ctcgccggaa atggtcagaa
     1321 cattatcttt caggctaatt ttgacatcat cttttttgac accgggcagt tcaacggtaa
     1381 caacgtattc cgtatctttt tcttcaatat caacttctgg agcaaaagtc actaaagatg
     1441 tttctgtatc ccaatcgaag aaaggatcga aaaaccgatc aatggtataa ggacttttcc
     1501 tcgttacgct ttttggagtt gctttagtaa ttgccatttc tacctccttt ttttattatt
     1561 ttttactttc tcatttcaat ttttcaaaaa ccgcttattg attactcaag tagtatgccg
     1621 accccaaaaa gcaccggata ttgtcagact atttgcaaaa aacgacaaag cactttgccg
     1681 atgtggcgat gtttggtaga attggcattc tttcctgaca gattcacgcc agaagggcaa
     1741 accatccagg ggcaaattgg cctatcatat atttcctcaa tcaaaggatt taatcataaa
     1801 tatgacttta ctttcttgtc cttttgacac tcaatctcta aattccatta gtctaaagaa
     1861 ttgaaaaatt atgggtattg tcagattaaa caacatggtc ttttatggtt accacggagt
     1921 agccgaacca gaaaaaatac tcggcggcaa aatcgaggtt gatgtcgagc tggaatttga
     1981 tatgactaag gtcatcacca cggatcatct ggcagacacg attgactacg aaaaggttta
     2041 tcaattagtt cagggagtcg taacccagtc caaatttttc ctgattgaag cattagccgg
     2101 taaaatttta agcgaaatct ttaatcatca tctggtagaa tcggtccgcg ttcgcattcg
     2161 caaacccaat gcgcccatca agggcgtgct ggatacggtc gaagttgaaa tatttcgacg
     2221 acgcgaccaa atgggagaaa aatcgtgaaa agttcttgcg tctttgtggc tctgggaagt
     2281 aatttgaact tgcgcgaaaa aaatttatca gccgctctgg aattactgtc gcataatcct
     2341 gcaatttcca tctgtcgtat gtcagcagtc tatgaaactg ccccaaaata caatcccgac
     2401 caaccgaagt tcctgaatat ggtagtagaa attaccacca gcttgactcc gttacagtta
     2461 ctcgaatttt gtaaagacat tgaagttcga ttaggaaggc atttccccca ctataagaat
     2521 tcacctcgtg aaattgacct cgatataatc ttttttaatc ggcttatagt tgaaagcccc
     2581 actctgacaa ttccccatcc gggtctctac gaacgtaaat ttgtgcttgt gccactggca
     2641 gaaatcgccc cagaattcat ttgccccaaa accgctaaat cggtcgcaga gttgctaagg
     2701 atttgtccgg atatcgagca agttgttaga atcggccaac tggatgaatt actgactatt
     2761 cccgttacta ctggagttct atgaagaacc tttacttttt agccattgaa ggcgttatcg
     2821 gtgccggtaa aactagcttg gcgcgtctaa tggctgaaaa actcaatgcc cgtctggttc
     2881 tcgaaaaatt cgaggaaaat ccatttctgg aagattttta ccgcgatccg gaaagatacg
     2941 cctttcaagc tcaactctat tttctcttga gtcgctaccg ccagcaaatc gaattgaaac
     3001 aaattgatct attcactaat cttttaatca gcgattatat gtttgccaaa gataaactat
     3061 tcgcaatcct caatctcgat gaaaaagaat tgtcccttta caacctggtc gctcaacatt
     3121 tggaaaaaga cttgcccaaa ccggacctag tagtttactt acaagccagc actgaccgcc
     3181 tgatatcaaa tatcaaaaaa cgcggccgtt cttatgaaaa atctatcaat cgcgactata
     3241 ttgattccct caaccagatt tacaatgaat tcttcttccg ctacaaagac agtccgctaa
     3301 ttattgttaa caccaatgaa attgacttcg tcaataatga aaatgatctg aatgaactga
     3361 tccgggaaat caagcaacca attagcggga cgatttatta taatccagcg aagaagattt
     3421 tataatgctc cgactattac tactttttct gatcttttac ctgctttgga aaatggtcaa
     3481 gccgcgcaat ctacgacagc ctaaaccgaa taatcccaaa cctaaaaaac cacgcacaac
     3541 ccttaaaatt gataaaagcc aaatcgaaga tgccaagttt agagaaatca aagaagagcc
     3601 agaaaaagaa ccaaaagatt gataaactgg caaatttgac tatttccgtt tttttcttaa
     3661 acatcctgat tatttcgtct gtattgatat tagtcttttt tattttcggc caaactccag
     3721 atgacaaacc ttccggttgg cgcactttga tcgcagaaca tttgaatcgc tacccggcaa
     3781 tgcaaccggc cgatgtatat aaattagttt accaaggaac tttcggaccg gcgcacttag
     3841 gtaacgattc aatccaaata tcaaccagtc tggaaaacga gctggcgatt attacaccgg
     3901 attcgagtgc cccgttatcg gaaagcatca caccttccgg taaatatgtg cgcctaaatt
     3961 taaaacgctg taaagccgag caaatccaaa ctgccaaaat cgtcacagcc atcttgaact
     4021 caatccccca atcttcagcc gattcctcca gattcattcg ccgctgggaa actatcgaaa
     4081 aattagttgt ggaaggctca ttgcctattc cgcggtcaga ttttttcact ttttccgata
     4141 gtatcaaaaa gaccggctac ccggtgattc atcattcaag tcaatatata aaaaattacc
     4201 aaccagcata tcgggtaatc gaccgtcgct atcttaatct aataagtgat tcattatcgc
     4261 aatgaaaatg gatttcattt taccttcttt tggactaatt gacgccgatt atctgattgg
     4321 cagttttatt ctcgaaatct ggcaaaagat atgaagaaat tagtcatcac cggcgccagt
     4381 ggtttcctgg gtggacatat tgcaaattta gcggcttctc gtttccaaac actcggactt
     4441 tattcgactt atcctttcaa tttaaaagga atcacaaccc agcgaatcga actttcctcg
     4501 ccgagttcaa tcatcgttgt ccttaaacga tttaaacctg ccgtcgtaat tcataacgcc
     4561 gctttggcaa accccgatca atgtgaaaat caaccggact ttgccagaca aaataatgtt
     4621 atcgctaccg aacatatcgc cagttggtgt cataaaaata atgcccgttt gatttatgtt
     4681 tcaaccgata tggtattcga tggtcagaaa ggtaattatt cggaaaccga ccgacctaat
     4741 ccgttaagtg tttatggcaa atcaaaatac gaagccgaaa aactcgcgat ggcaaataat
     4801 cccgatacgg tcgtagctcg tctggccttg atgtacggtc gcggaatttt tccgcgtgct
     4861 tacaattcag aatggctact acgagagcta ctacggcgca aaaaagaacc gaaattacca
     4921 ccgttgaaac tttataacga tcaataccgc agtatgttga tggtcacgaa tgccgctcaa
     4981 gcattaatcg agctggcttt atcacatttt tgtggaataa tacatatcgg cggtccggaa
     5041 cgcatcagcc ggttcgcctt cggcgagaaa ctttgccgca agctaaactt acccctgact
     5101 tttatccaag ccgttccata cgacgaacat caattaacag cgccgcgacc acgcgattta
     5161 agtttagata tttcgctagc ccgttcattt ttgaaaacaa attttttagc ggttgacgac
     5221 ggtttggatg aaatgctaat gagtaatagt gattaaaatg gaaaattatg gattctgtcc
     5281 aaaaaattac tgctttgact atcggcaagg tttgcatcaa gccgccgctg attttagcgc
     5341 cgatggctgg ctataccgac caagcttttc gccagatttg taaagcgaat ggagccggcc
     5401 tggtttgtac cgaattcgtc agtagtgacg gtctggtacg cagttcagaa aaaacttatg
     5461 actatctacg tttttccgac ttcgagcgac cgattagtat gcaaatattc ggcaacaatc
     5521 ccgtggttat ggcggaagct gccaatttga tagagaatga atttcatccc gaccttattg
     5581 acatcaatgc cggatgctcg gtccgcaagg tcattaagaa aaacgccggt tccgccttgc
     5641 tgaaaaatcc tcctttactg gaaaaaattg cccgtgcggt tgtcgaagcg gtatcggttc
     5701 cggttacggt aaaaatgcgg agcggatggg atcgccaatc cattatcgct gttgaaactg
     5761 cccagcgatt ggaacagacc gggattaaag ctataacaat tcatccacgc actgctgttg
     5821 atggttttcg ccatccagcc gattggtcac tcatcaaaga aataaagaaa aaaatcagtt
     5881 taccggttat tggtaacggt gatgttttta cgcccaccgc cgctttacga atgttcactg
     5941 aaaccaattg tgacgctatt atgatcgggc gtggggcact cgaaaatccc tggattttcc
     6001 gccaaatctc agatttactg gaagataagc cgctaatttc ctatgattcg cccaccgaac
     6061 gagtcaaact ctgtcgccga catttagaga tggagatcca atatcgcgga gaagtcgtag
     6121 ctcataaaga aatgaaaaaa ttctaccgct ggtatttgcg cggcttcgga cacgtctccg
     6181 aaatccggga aaaattggta cgaagcaaat ccgtacagga agcatttaat attctcgatg
     6241 aattggctca cgttacaaaa taagaaaggt taatgaattc attaaccttt cctgaacctt
     6301 aatttttaac gactgcagag ttatttaaaa tagaaagcaa gcacgatgct cgccgtcgaa
     6361 ctgccgagaa ttcccagatg gacgtcagtc atcttatcat cattttcctt atcatttaat
     6421 aagtcaattt ccagacctac ccaattactg gccgataagc tcacctgatc gaaaatccac
     6481 cattcgacac ccaccgagcc acctaaccac atataagtcg aattgatatg aatgtcacca
     6541 aaatctttat cttttttgct agaaaaatta aatccgacac ccggagcaag atataacgcc
     6601 accggacctt tctggaatgg gtaatggatt aagtatagat ccatacccag cgctgtagtt
     6661 ttttctttct cttctattcc atcatcctca ctatcatggg aaaaattcaa ggctatcccg
     6721 gcaccagttt tgtccttaaa caagtaattt agcccgatcg cggaattctg gtaactcata
     6781 tcgctcaatc cgttaaaatt aaaccacata gccgccgaac ctttctgggt cgtcggcgtg
     6841 ccggcagtcg ccagactcgc taaaatcaaa atcaccacca cacaaaaaac aacaagcgtc
     6901 ttcttcattt ttaacctcca ttgtttgtta aagacttcat tgtcatttat aataataaca
     6961 atttttcttg ttcatcacca gcgacgacca cttcccggtc tttggttatg aaaacattaa
     7021 tctccgaccg caccgccatt tcacccggca ggtaaatccc cggttcaatc gaaaagcaga
     7081 taccgggcac cagttcgcgc tcgtccttcg tttctaaatt gtcaatattg acgccattgc
     7141 catgcacata atgcccaatc gaatgtccgg tgcgatgaac aaaataatcg ccatatccgg
     7201 cttccttgac tacattccga caggcgtcgt caacttgata accgtaacaa ggctggttgt
     7261 cagccatttt gcgacggata aaatcgcgag ctgcattacg agcgcgcacc acgatattaa
     7321 aaatttcgac gtatttagca ggcggatttt ggccaacaaa cccacaccag gtaatatcat
     7381 aataaattgc ccgttcggat tgttcgcgcg cccagaggtc tatcaatacc gtatcgcccg
     7441 gttttatcgt gtaagcattc tccgccttcg gctcaaaatg cgggtcggca ggatgttcat
     7501 tgactcccac aatcggatgc tcgttgtcgt ccgtcaaatc ttcttcgccg aaacgtttga
     7561 cgataaattg ctgaacttca aattcggtaa cagatttttg ctcttttatt cgtgaactga
     7621 ttagggcaaa ggcttcattt tttatctgct gaactttctg accagctcgc aggtgcgact
     7681 gatagccttc ttcatcaata acagcctcaa aaatttgtac gagattagct gaagaaacaa
     7741 tttccacccc aaaagagcga attaattcaa tcgttccacc gtcaacaacc gaaatgtagg
     7801 gtatgttatt attcggcgaa tattgcatcg cgattcgccg actgtttttg agcatagtgc
     7861 gaagactatt atgttgctgt tcccaactgc gatagataat tttttcaccg gggagattat
     7921 ctattttagt cgcttcgacg gccgaaacaa gttttatggg aatgccttct gccggaatga
     7981 aataatacca gcgccgggta acgaacatat tcggatttaa gcccagaata tggtaagcca
     8041 ttttgtcacg gttatgaaaa tcataaaaaa gccacccgtc aacgttctgt tggcggattt
     8101 cggtttgaat ctgttcaatg ttcattaaaa taacctctat ttaatgatct tagcaatttt
     8161 cttaaaggcg tcattcgaag catctttcag catctcaaag agtgccattt cgacactcgt
     8221 tgggtgaacc ccgtaagcca gcaatttgct taccgtcagt tgcgtatttt cgggcttgcg
     8281 gctcgcgatg caatcggtta ccaaatgcac ttcatacttt ttatcaatca aatcccggca
     8341 ggtctggtaa acacaaatat gggattcaat tccacaaacg atgatctgat cgcggttaat
     8401 tttgtgaatt tcattcataa aattgtcttc actgcaacaa ctgaatgcca tttttataat
     8461 cggtggagta tttgataata acgaggcaat ttcaggatga gttggcccta acccggttgg
     8521 atattgttca gtatagataa tcggtatatc cagaacctgc gaaccgcgga ttagtttttt
     8581 aagattctct agtaagagcg gataattgta cataaccgat aaaagcttcg tttgtacatc
     8641 aataatcact aaaactgatc gttcattttt tagcttcatt gctcgaccct ttctattgga
     8701 acattgtttt tagatttagt tattttagtc ctaaggttgc gaaaatgtaa ataaaaaagt
     8761 attccaatta gaaggaagat cagaattaag tttgttattg atttccgtct ccgactcatc
     8821 gctaccatat ttctttaatt aaatatggaa tatttagaaa aaataaacaa taaaaatatc
     8881 gtcattttca cttaaacatt ctgaatattt ttggctgatt tagtcatcat attcaacgat
     8941 tcggaatcat ttgaactttc tattgagggt gtcgcgctgg cgctatctgg agcttgtttt
     9001 tttaattgat taaaatagaa aacacccaaa aaccacgact acaatctgat tattgcaact
     9061 attttaataa taaacaattg actttctgat aaaacactat taatattagc aaggtatttt
     9121 acaatattaa atacggagga ttttatgaag aatttagtat tgatttcagg cgttctggga
     9181 gtattgactt tccttatggg agttatcacc caattagttg gtcactctat cctgttctgt
     9241 gccgcgagtt ggaacagcct gactcaaacg atgttactat tcgccattgc ctttgctgtt
     9301 tgggaacatg aaaaatgtaa atgtaaacaa gaagagaaag agaaagagaa agaatagtaa
     9361 ttagacatca tttcttaaag tcccggtaaa tccgggactt ttttttcgcg gaattattta
     9421 ttttactact ggttaaaaac gcttatttta tttgcaccca gactcgtcgg ttacgcggtc
     9481 cgtcgtattc acagaaaaaa attccctgcc aaacgcctaa caacaaattt ccattattca
     9541 cgatcagctg gacactattg ccggttaaag ttgacttgat atgagcgtcg gaattgcctt
     9601 cgatatgggc gtaattttca ttatggggca ccagtttgct tagacagtta acgatatcgc
     9661 tggcaacatt cggatcggca tgttcattga tagttaaacc ggcagttgtg tgtggacaat
     9721 agacgacaca tattccttcc cggattccac tggagcggac agcttcagcc acttgagctg
     9781 taatgtcaat tagctcgata cgacgattgg tttttaaaat aatctctttc aattttcatc
     9841 cctatcttaa gttttacgtt ccttcttacg ggctgatgga aaaagtacat tattcaatat
     9901 cagccgataa cctggtgaat ttttatgtaa ttccaaccgg gtaggcggat caccaacaaa
     9961 atgttgataa tcttctggat cgtgtccggc aagaaaggta aaagttccct tgccatgatt
    10021 gccatggata tagcgcactt gctcaccgtt aacaacttca cccataacga caaccgattt
    10081 tttaatccga tcgcggcgaa atccggttgt ctgtcccata aatcctttaa ttactccgac
    10141 atgattttga gtcaacattg ttggcaccgg atcccatttc gctgaaaatt caaataaggt
    10201 aaaataatcg gcttcggcac ccggcgctac ggcttgaaag cttggaggat aatcaatatc
    10261 ggaatattcg tagagcatcg gatccagata caatttaaaa ttctcaaatg ccaggcactt
    10321 ggaataatcc agtttttcat tggcgcgtgg atcagccgga tcaccgtcgt aaatttcggc
    10381 gacaatatcc acattctcag ccgccaatgc gatatccaag gcatcggtag ccgaacacat
    10441 tgcgaacaag aaaccacctt cattgacata tttctttata gtacgcgcta cggctttctt
    10501 ttcctctgaa actttacgga agcctaactt agcggcgata gcttcattct cagcgacttc
    10561 gttttgatac caagcttgat tatgataact cgcataaaac tttccgtatt gcccggtgaa
    10621 atcttcgtga tgcagatgaa gccagtcata ttccggcaat ttcccagtta gcacttcttc
    10681 atcccagaga acatcgtaat caatttcggc atacgtcaga accagagtta cggcatcgtc
    10741 ccaaggctgt ttattgggtg gcgaataaac cgcaatctta gcggcttttt ctaatagcac
    10801 aacgtccata ttattttctt cgatcgtcgc ataaatttgc aggttttgag cggcgctaac
    10861 ttcttcatag gcaactcctc gtaagcgtag ttctttaaca atgcctggat aattatccag
    10921 aagaaaagag ccgcctcgat aattcaacaa ccactcgaca tttactcctt gcttcaaact
    10981 ccagtaaaca atgccgtaag cttttaggtg atcgctctga gtttgatcca tcggaatcag
    11041 aattttttgg gcaaaactca gattcgccag gattaaaatc agtccaatta atacaattaa
    11101 aggccggttg cgcttcattc gattcaaatt tcttttttta cgcctgacaa gcgttgcagt
    11161 tcgcgtagac gcttgcgaac attatcaatt aaaaatgaat taggatattt ttcaagaatt
    11221 aattcgtaat agtaagccgc cgcttgattg tttgcctcac gatagtcggc aacttcagcc
    11281 agcataaaag cgatttggtc agcattttca ttttcagaag tgaaaaatgg ttcggcggtc
    11341 gcctcggctt cggcataacg attaaactgt aaaagaatct gggttaaacg aaaccgagcg
    11401 gctgaaatga tcggggtttg aggatagttt tgtaaaataa aggcataaat cttttcggct
    11461 tcactcaatt tattttggtg taaaagaagt tcagccgtag cgaattcagc aaaagctttt
    11521 ttcccagcgg cgtcggtctc gctataattt gcccggaaaa aaccatcaaa gccgactacg
    11581 tcattaaaaa gcgcatgctt tggtcccagc aatggtatta aatttttcag ttcgatgtcc
    11641 aatgaatcaa attcctgcgc caaaaattga gctagtgcca gatgaactcg taaatctttt
    11701 tcacctggtg ttccctccca tcgtacaatt tctgcttgat aatatttaat agccgcgtcg
    11761 ggatcgccgc gcgcaatctg cacctcgcct attcgtaaag cacagtttaa aattaattgt
    11821 cggtcgcggg tgatattctg agcctgacgc agtagattca gcgctccatc aaaatcacgc
    11881 actgcccgaa aacgcaaatc tgccagccga tacaaagcct gagctgaata gatgctattg
    11941 ggttgattgg caactaaaga atcataaatc gcaaaggctt tctgcaaact cgattgttcc
    12001 tgattaattt gctggacaaa gtcggtattg aaaaagaagt taccaggata aaaataatgc
    12061 aacggctcag ccgttgtctg ttccagccca gctttctcga atgactcagc taatccgagt
    12121 aaagcttgcg gagcgatacc ctgcaattcg ttctttgcca aaattttcgt ataaaaatcc
    12181 tgggcgagtt cgtaacgccg aatctggact aaatttttag ccattgccag cgcttgatct
    12241 ccatgattac cagtcagcgc ttcaacctgg aaaatttcct ccagtgcctc attataatgc
    12301 ccgtttttaa aaagaaaatc cgcccgcagg cgatgcagag cggcttgatc gggcaaaagt
    12361 aacaactccg cctttagcaa ggaatctatc tgaataaaca acgtcgagtc ggtcgggaaa
    12421 cgcaaaattt gtccggcaat catattgtaa taacgcggat tggcgcgagc ataaagtaca
    12481 agttctcttg ccgccgagct atattttccc cgtgcaattt gcaaattggc taattccatc
    12541 gtcaataaat cgggttgtct ataatgagcg cgggcggcgg caatcaagcg ctcggcttca
    12601 tcatataatc tcaagccgac catttcattg aacagagcac gataaaacga aacgccatgg
    12661 ttaaatttca cgacggcttt ctgccattcc tgaaacgctt cagcgctgtc accgcgcaca
    12721 tagaagagtt taccgagatc aatagcagca ttttcatgaa gcggattcaa ttgcataaaa
    12781 cgggtaatga cacgttcgag ctccggtaaa tttttctgcc ggaaaagcaa gttgacataa
    12841 ttgctataat acagataatt agccggttgg gcatcaacca acgcccggta aatttcagcg
    12901 gcttgcgtca actgaccttc ccgctcgagg cgctgagcct gattaaagcg taaattcatc
    12961 agtttttctt cgggtgaaac gacttttacc ggttgagcgg caacgaaagc tgtaagcgaa
    13021 aaaattaata agattaataa tccccaacgc cgtccgagct tcattccacc gactccttac
    13081 tcaattcttc aaaataccgg cgaatcagat tttcgtattc tttggaatac ccgtcgcgca
    13141 gagctttttc tagattttcc tgtaaaagag tgcgccgctc gccaaggtta gacggcagac
    13201 catcaggaga ttcgcgaacg atttcttgcc cggttttact ctggcgttcc tctttaaaat
    13261 cctgagtgcg cagagatttc tgagcatcca ggagtaggga gagaattctc tgctggcgtt
    13321 ccagcgtgcg gcgtaaaacc tggttattct cgaggtcttt gatgacttcg tccatatctt
    13381 tggcaatgcc accgagatcg cccgactgtt tagtgctacc ttgcccttgg atttcatcac
    13441 gtaatttttc gagtgattgg cgaatttgtt gctggcgagc ggctaattgc tggagagttc
    13501 cctgatcacc gggagaaccc aatcccaaca tctgggtttc ttcattcaaa ccctgttgca
    13561 taccagccaa ttcctgcaat tgctgaaggt aattttcgaa accggaagcc gaaccgctct
    13621 tctgtaattc ttgcatagcc gccatcagtt ccagagccgt catattgagc gaagcggtgg
    13681 cattttcagc ggaaagtgcc gccagtcccg gatctcgttc ttccatatag gcgatagccg
    13741 cattcatact attagctgat tgaccgaggt ttttgccgat tcgcggggaa acgccaaaag
    13801 ttttacctga caaatcaatc aaatcggaaa tcaggcggga caaattctgg cgagtttcac
    13861 tttgcttaac ggctatatcc attatctgat cgctttgacg cggagtgttt ttaatctcct
    13921 cggtgagttc ttcttgacga taggataatt gcattgtctt attcaaaatc cgacggaagt
    13981 ccgtcataac gtccgccata ttccgcctct gaaattcagt gcgaaattct tccattcttt
    14041 ctaacaattt ctgcaagttt tgctgagaat tctgggctga tttctcggct tgcgatttat
    14101 cacctttgcg catcgcactg ctggcttgcg actgttcttg cggcagattc agagcttgca
    14161 tttcatctat taattgctga agttccttat tcggcatcat cggaaattct gccatatcag
    14221 ctgcagttct tttcataata ttctgagcaa tttcagtatc atttttaatg gcattttctg
    14281 cctgcgctaa ttcattcaat ttttgctgat cattcggcgc tgttttcccc agctcgttag
    14341 ccacttgcct ctgcctttcg ctcagatcgg caaagcgcct aaccaattca tcaatattct
    14401 gttcaatttg aatccggcga aaaatcttca ggaggcggtc gagttcctgc gaaaattgct
    14461 cctgggaagt tttgaaatta tttagagctt tttgaacttc ctttggatcc attttttcga
    14521 tagcggcttg caatttatcg agtgcttcac gcatttccgg tgtcattata tcctgaaatg
    14581 cttcctgcac ctgggctaat ttttccaatg tttccgcatc gaagagttga ttctcacgac
    14641 ttttctctat caagtcatcg agttgctcgc ttagtttggt gattttttca ccggcttcgc
    14701 gagttttttt catctcctct tctaattgct ttttctgttc ccatttcagt tccggttttt
    14761 tgagtaattc acgggaaatc tcgtcgagaa cctttttggt agtttccaga gattttaaaa
    14821 cttcctcgct actggaataa atttcatcct gttgttcgtt gaccgagcta aaaagttcat
    14881 tgagtgatgg aaagcgggcg attagccagt cggtgctggc ttttttgggg ccgctaatat
    14941 tatcattgtc ataaatatca acccggaatt cgactgcatc ttcaggacct aagctaagtt
    15001 cgccgatttg ccactgaaaa aatacttcct gccaagtgat attcaatttt gcgagtggga
    15061 atgttatttc cgtttcctct tcctgataat cggcatattt ttttatcaca cggtagcgaa
    15121 tagcggcgcg ggtgaatccg aaatcatcgc taatccgaat accgagtgga attaccatat
    15181 cggcgtttaa atcaagatca tttttcgggc tgagcatagt gatagtcggg taggcatccg
    15241 gaatgacgct tagtcggtac ttgataggat taatatttgt cacttgcttt tcatcggcta
    15301 caataatttc aaactggtcg tcatttttta cgatgaattc tccacgagcg cgatcaccat
    15361 tcgtcgccaa atttaagaca ttcccgctgg caaaggataa atggccttct ttaatttctt
    15421 tattaatacg aatttctaaa gaaattttcg tgccagacag caagttaaat tccgcgcttt
    15481 tggtttcctg agtctgagcc ggaagtccgg tatattccgg ataaataaaa cgggatttga
    15541 catagagaat ttcaggtcgg ttgatgactc gaattgtatc gggaggagtt ccgattcggc
    15601 gccagggctt gaacggcgaa tgattttgat aataagcgct ataaaccaga tcatgccgca
    15661 aattttcgat cagatattgg ctccgtccgg aatcatctac cattaaaatc tcttgccgag
    15721 tgtaatccgg atactgaagg tcaagcgtta aatagtgcgg aaatcgtccc tgacactgaa
    15781 agctgattgt taccgaatcg ccacctaaca cccccgatga cgctcgggtt gattcgatag
    15841 aaaagggcaa tttgaccggg tactcgcttc cgggatgcaa aagtcgctcc atgctatttt
    15901 tgaaatatgc gggactggag gcgatacaaa ttacgaggca aaaaatcaca attgccagat
    15961 tacgcaaact cttccggact tcctgagatg gtattatccg atcaaaatta agcggctcta
    16021 tttttcgggc tatccgctcc agagcttgct cgatcagttc actcgaataa ccttgtgtct
    16081 ctttattcag caactctctt aactgcaatg cattcagtaa atcgtccttg atttccggct
    16141 ggtcggcgcc tatccggcgt gccaggtgat aatcatcgta agccctaatt cgattccgga
    16201 ttatttggaa ataatacaca attggaatcg ccagaaaaat gaggctgaat ccaaccgtaa
    16261 aaaataaaat gtcactgcgg aaggaacctt tgaaatagcg aaaaccttcg acgacaacaa
    16321 ttaaaaaagt cacgattata atcaacaata aaactttcag caaaccgacc tgtaaagtat
    16381 gccgcgcaaa tttgcgtcgc aagtcgttta atttgcgttc gatccggtta tagctggatt
    16441 gattatttct catttttatt gcatcaacga atataaaact atattggcgc ccatttgaag
    16501 tgccgcctgg tgtttttcct caggatcatt atggacttcc gggtcttccc agccgtcgcc
    16561 gagatcacac tcataagtat aaaacacaac cagtcgacct ttgtaaaata ttcccagtcc
    16621 ctgcggtggc ttgccatcgt gttcgtggat tttgggcaaa ccgtggggaa atttatataa
    16681 aatattataa atcgggtgat caaatggcaa ttccaccagg tcattatcgg gaaaaactcg
    16741 tttaatttca cgccgaaagg attgatccat accataatta tcgtcggcat gtaaaaagcc
    16801 accgttttgc aaatgctggc gaaggcgcag aacctcctca tcactgaaac gcacgttgcc
    16861 atgaccattc atataaagat aaggataggc gaaaaagtct ttgtccatta taccaacccg
    16921 cacttcgtcg gaggcgacgt ccatattgag attttcacgg agaaatttca gcagattcgg
    16981 aattgaactc ggattgctgt accagtcgcc accgccgtca tatttcaggc gagcaatcgt
    17041 aaatttccca tagagattct tactctgccc ggcaagattt aagaccgcca ataaaataac
    17101 gataaatgtc caactaattt tcttcaattt cctgcgactc tcgcataata atcataataa
    17161 atttactata cgcttgagct ggtaacaagt ttcgctcaag aaaaaacaat cctcttcatt
    17221 ttttcccgag gtattgttat tttctatccg tggctcgatt cgtaaaatgt atcttaataa
    17281 tcggggtatt aaccgttagc ttatccgcac aatggtattt caagattgtc gatgctattc
    17341 acggaccgat cgaatatcgc acaccattta aattgacact attcgaattt aaaaccggca
    17401 tacagacatt tggatttgac ggtcaaccac ttctatttga ttcccccgga atttccttta
    17461 acggctataa aacttcttgg gaacgagcga tgaacagcct cgaactggat ttgttgaagt
    17521 tcaactggct ggtgtatatt ttgccgcaaa atttgctcga tttccagacc ggaataggcg
    17581 ctaaatacac atactcatta tccgaacaaa aactcccgga aacctggccg caacgtctgc
    17641 cgggctccgt tgaccaactt tttttgtcac cgcgaatcta tgaattcaac ctcaatcaaa
    17701 cagtgatata tcaatgggcg ccgaactttt ataattactt aacgatcagt tacggtcaag
    17761 cttccggctc agcttataaa actcattcta acgactattt tctacatcaa aaagggcaca
    17821 cctactcctt cgcgctggga tttaaattcc tcggcagtat caattataaa ttaaaagaag
    17881 gttacggcgt agaaatcaga tacacagtcg gtgatttcgg tgatttggaa gaccctcata
    17941 acataagccc gattaagaag attaatttta atactgtggg gatttctctg acattcaatt
    18001 ctaatctcgg cggaggtcgg agtactggtg acgaagccca agctctttat caggctaatg
    18061 attttatagc cgccaaagcc acctttgaac aattcatcgc cgataatccc caccacccaa
    18121 gccgctttaa agcccgtcgg atgattaaag aatgcgacaa gcgaattccc tatcaggaag
    18181 taactctggc ggaattgttt attgcccgtc aaaactattc catagctact gaatatttag
    18241 cgcgagccca agtgactaaa gacaaaaccc tattagctcg gataaatagt aattacgaaa
    18301 gaatgattaa acagtttgtt gatacaatga acgtgatgat tagcaacaat cgaatcgatg
    18361 aagccgaaca atttttaaac acaatcgcaa agctgaatct cccaaacact aatgagctta
    18421 tctaccgcta ccacagtgaa atttatttcc atcgcggagc agtttttact gaatctggtt
    18481 attgggaaaa agctattgac tatttcgatc ttgccattcg atattatcca cctattcgac
    18541 aacgcgtcga accctatctg gtacaaatcg ctgacggtta tatcaatgac gccaaccttt
    18601 ctgtggataa gaaaagtata gcgttggcgc tggagtcgct caagcaagcg accgctctgc
    18661 gaccggatat tcattactta actgctcctc atattaaaaa cttagaagcc ggtatcgaat
    18721 accttaaaca gcaagccgcc aaacaaaaag cacaggaatc cataggtaaa acattcaacc
    18781 cgccgccgga tgctcctaaa cccgacatcg gtatgagtac tgatcaaata agaagctcgc
    18841 ttgggcaacc aggttcccaa acccgcttag aaaccggcgg tggaagaatt tacgaactgt
    18901 ggatttactt atatccagat ggcaaagaat tacagattta ttttgataat gcaaaggtgg
    18961 ttaagatgga agctttatct ccgattgacg taatttcaga aatggacgaa tagatgaaga
    19021 tcatttactc cgacggagcc tgttcaggaa atcccggcca cggcggttgg gcgactctga
    19081 tttttaatga aaatcaattg ctcaaagtcc tagccggtta tcaagaaaat acgactaata
    19141 accggatgga aatcatcgcg gcgctcaatg gcttgaaaca attgcaaccc ggtgaaaaag
    19201 ccattattta tactgacagt caatacctac gtaatggaat tacaacctgg cttcccaact
    19261 ggaaaaagag aaattggaaa accgctgaca agaaatcagt caaaaatcag gagctgtggc
    19321 tcgaacttga caggttgaat catcctggaa tcacctggca atacgttcga ggacatagcg
    19381 ataatcttta taatgaaatt tgcgattgtt tggcacgtaa aatgatcgca agtcaaggga
    19441 aagtaagcga tctaaacgcc gaattcgaaa aatattactg gcaattgcag aagaagaaaa
    19501 agcagtaatt atttatcaat tgatgttgag aaaacgcgct acgatcgttt tgcgtccgca
    19561 ggaacgaaaa tttacggtta ctcgggcatt ttgtccgaca ccgtctatat taactacctg
    19621 accggtgcca aaaattttat gagaaacatg cgtaccgggt tcaatcgtcc ggctggtcgg
    19681 agaagttttt tcgcttgaag aagaattgat tcccgctatt ttgatcgggt tagattcaac
    19741 ccgctcaaag gtatctttta ttttccgagc agtggaacga ttgctggtcc gcggttggcg
    19801 cttactggaa ataatacctt cccgaaaatc gcagattgta atcaactctg gcgggatttc
    19861 gttgagaaac attgattccg atgagtaggc gacttcaccg gcgcgcatac gctggcggga
    19921 cgatgataaa aaaacctttt ccttggcgcg agtcagaccg acataaaaaa gacgacgctc
    19981 ctcttcgagc gcttcatggg tcaagtcgcg ttcgagcggc aataagcctt gatttaatcc
    20041 gcaaatgaag accacaggaa attccaagcc cttggcaccg tgtaaggtca tcagcgtcac
    20101 cgcattttta gtgtcttccc attcgtcaat cgaagtcgcc agagtcacat tttcgaggta
    20161 agattctagc gaaggctttt cgttagtctg gcaaaattcc gaaatatcgt tgagtagctc
    20221 gtcaaggttg gctaatcttt cagttgcttc ttcgccctcg ctttctttgt aatagttacg
    20281 caggccaatg ctgacgataa caacccgtgc ccattcttca aaagaaagtg tattgcgtaa
    20341 tgaacggaga tcattgatct gatcgtaaaa ctttttgata gattcgatag cccgcgtagc
    20401 aagattgagt tcatcggcgt acgacatagc atcgaatagc gaaatgtctt tagccaaagc
    20461 cagttcccca agatgttgaa cggtcgtttt acttatcccg cgtggcggta gattgataat
    20521 ccgttttaaa ctgatatcat catggggatt ggatatgacg ttgaaaaacg ctaaaacatc
    20581 tttgatttct ttgcgctcgt aaaaacggac accacctaca attacataca cgatgttatt
    20641 gcgccgtaaa atttcctcaa gaacacgggt ttgggcattt gttcggtaaa ggatggcaaa
    20701 gtccttgaaa ttacgtttgt tctgtaaaat ttctcgttga atccagctgg cgatcagggc
    20761 ggcttcgtcg tattcggtct cggcatccag cttcactaat aaatcgccat cgcttttctg
    20821 tgaccagagt gtttttgtag cgcgctgttt attgtggctg acaaccgctg aagcggcttt
    20881 caggatatta ttggtcgagc gataattttg ctcgagctta aaaacccggc attggggaaa
    20941 tatttcgttg aaactgagta tgttgcgaat atcagctccg cgccagctgt aaatactctg
    21001 gtcatcgtcg ccaactacac agacgtgctg atgtttttta cccagggctt caatcaagag
    21061 aaactgggga cgattggtat cttgatattc atcaactaaa atatattgat aaagtgcccg
    21121 gtatttttcc aaaacttcgg gtttaagctg gaatattttc agcggtagaa ttagcagatc
    21181 gtcgaaatcc accgccatag ctttacgtaa agctccctga taaaggggat atagcacagc
    21241 tacaaaacga tcagccataa atccgggccg agcgctgaaa ttttccggct caatcatatg
    21301 atttttcaga taggaaattc gactcaagac atttttagga ctggtggact cgaggataat
    21361 agaacgatca ttgataattt tcttgagaag actgagctgg tcctcagcat cgtagattgt
    21421 aaaattcgaa gaataaccga gacattctat ttcacgtcgg agaagacgag cacaaatcga
    21481 atgaaaagtg ccgatattga cttgcctggc tttcttgccg atatattttt ctacccgttc
    21541 acctaattcg cgtgcggctt tgttggtaaa ggtaaccgct agaatatttt ggggggcgac
    21601 tattttattt tctatcaggt aggcaacttt atgcgccaga accttggttt tcccactccc
    21661 ggcaccagca aaaattaagg ttggaccagc attatattcg accgcacggc attgctcagg
    21721 attcaaatcc tcaagtgatt tcaattgata taatcctcac tggcaacaag tttatcctgg
    21781 gtacggattt tttgcaattt tctttttgct gctaaaaatt cctcataatt cgcggtaaaa
    21841 gtgtgggtat aggtactatc accgtttgaa accatatata gatatttggt tttggcggga
    21901 taaatagccg cttttatgga tttgaaaccg ggattacaga ccggccctgg cggtaatccg
    21961 tagtgtaagt aagtattata aggtgaatca atttttaaat cttgtcgcag tattcgtcgc
    22021 ggactatccg gaattatgta ttgaattgtc ggacaggctt cgagacgaat ccttctacgc
    22081 aagcgattgt aatagagtga ggcaactagc ggtcgttcga aatctacctg acattctcct
    22141 tccactatcg aggctaaggt aataatctgg tgaagagtat aaccagtacg acggatttca
    22201 gcctgcactg aatcattgac aacttcccag aagcgttcta ccatccgact aatgacattt
    22261 tccggcgaat cacctacgat aaaatgatag gaatcaggaa agagataccc ctccagatta
    22321 ttagcttcaa cccccaattg atgagtaaat ctggaatcca taactttttg catgaaatca
    22381 tcggccgaaa ttccaatctg attttcaagc aacctagcaa tttcacgggc acgcaaaccc
    22441 tcaggaatag tgacgcgcac cgtaaccgat tccagatcac tcagagtttt tatcaactca
    22501 taataattga tagctccata tagatcaaaa cgtccggcgc ataatttccg ttcctttccc
    22561 atcatttttg tcgccagaat aaagcgttta ggatacttga taataccttt ttcatataaa
    22621 agctcagata cttcctgaac acttgctcca ggttgaatag tgattacctg attggtatta
    22681 cttgaactca atggagttac taaaattgct aaatacagaa ttactataaa aaagattatc
    22741 agtggtatat aaaccaatgg tcgtttaacg gatggcttaa aattggattt tatacgcttc
    22801 atactatcat aattctattg cttaaatgga actggaataa acgtcaggat aaaaatcact
    22861 aaagatgcca gtccaatcag tttttcacgt aatgttaccg gagaattatc atcataaatc
    22921 ggcggatgtt ttactctaac caagaaaaac actagtaagc cccacactaa ccaattaagt
    22981 gacacaaatg agagcgctaa agtcgcaatt aacgctcccc aagcaacata aggatattta
    23041 tcgcccagaa gggcatacgc aatatgtccg ccatccagct gcccgatagg caagagattc
    23101 aacatggtta ccagcattcc gatccaagcc gcaaaaccaa ccggatggag caaaatgtcc
    23161 tgcccgactt ccaggtttgg aaaaatcaaa gccgaggcaa ttttcatcaa tagtgaatca
    23221 ccgagttgaa taccgattgc ctcacttaac gatacgactt cagaaagctt taagccaatg
    23281 aataaagctg gcgtcgctac caaaaaaccg cagatcggcc cagccgctcc aatttccaat
    23341 aaagcctgcc gccgcgttac cggagagcgc attttaatga aagcgccgaa agtcccgata
    23401 atagttggcg ctggaataaa gtaaggtaag gtcgcatcaa cattatgttt acgcgccatg
    23461 agaaaatgcc cggtttcatg aaaaccgaga attaccatca gagtcacact aaaaggaaca
    23521 cctttgagca aatccagtgg atgaacgaat ggattagccc cctccattat cgcccccgct
    23581 aaaagagtcg ttaatatcgt tatcaaaaac aagacaagat taaccgtcgg aatccgtttt
    23641 tcaattttct ccggcttgac gataaatcgg tcaatcgaac cagtagatat tttttttatc
    23701 gagctaatca aatttcgaga attgcgggcg attttacgca aagaagtggt atcccgctgg
    23761 cgcccttcga tcaccagata cttatctcga tcttctattc ggacaatttc gatccactga
    23821 ctcaggtata agataatgtt ttctttggta gttatatcca gcataacatt tcgtttaaat
    23881 tacccaattt ttagagaact tacaaaatac agccttaaaa tcaatcaaat aattcttcga
    23941 cttttgtctg gcaacttatc agtttctgct tctaaatgat tgaacttaat gattaaagat
    24001 attcacgtat tatgaaaaat tcgaccgaac tttagacacc ggcagactaa aaccggttac
    24061 aataacctac ttgggtaaag ttcacatttg aattcaggta tcaaagaaaa tgaaccagaa
    24121 accgcactga atttcatttt agtttactct acatgaaatg catagtagcc acatattatc
    24181 tcaataacac caatttttga cgtgcccgtc gttcaccctg acggaaaaca ataaagtaaa
    24241 ctccgctggc aaccggattg cctttttgat tgtgaccatc ccaagtccag acattcaagc
    24301 cgattttcgg attattgatt ttttcggagt aaacttcctg tccgttaaga ttgaaaatca
    24361 gcgccgtaac tggcttttcc acagtcaatc gaaaagggaa ataaatcaat ggattttcac
    24421 cgggcagaaa tggattcggg aaaggattca ggatagtaaa gtgggttgct gtctctcctt
    24481 gccagtcaga ataaatacat tcgtatgcta aagtatctac tccgtcgaag aagaaaaagg
    24541 ctaaaatgct atcgctcgcg gctgatcgat ccaaaaatac ttgcacatca attgtatcat
    24601 cttccggaat gaatccgctc gatggcaaaa aagccagaca ggcttcttcc gaccagaaag
    24661 caaattggct agaacctatg ccaatattga aaattggtaa tgttgtccgg ctaatatcat
    24721 cgctgaatac catttctgtg ggaatgagag cagattgcgc tgttgaggta tttcgcttta
    24781 aaacactaac atagagcaat tcatcgccgc cgttcgaaat aatcggaata ttaatgtcgt
    24841 gaaagccgct ggtcaataaa ttacggtcaa tggttaccgc aatttcagca ctcccaatat
    24901 tcactgtacc gaaaagtggt tcaattttca cccaattcgg cagttcgccg gaaatttccc
    24961 attccagcgc ctcggtaccg ctattgctaa tccgaaacgt ttgagtatat tcatcatgcc
    25021 agaaccacaa actatccggc gtaagaataa tccgcggagg tatcacgatt ttccccgaaa
    25081 cgatggcacg gatagcccaa ttgccggtaa gagaaacatc ttccgaaccg ccggtcacag
    25141 tataattgca gaggtcgtga aatatttcat cagttttgat ataagcatga ccgaatccgt
    25201 cttgcgcaga actatagccg agcgagttac ccgaactggt aaccgatacc gcaaatgaac
    25261 tcaaatcggc caagtcaatc ggattgccga gaataatctt tgtccaagcc tgttcatcag
    25321 gaataatgcc actcatagtt aatagcggcg cctccgctaa attgctgtat atttttatcg
    25381 taaccggact ggaatcatag gttaatattt taatcgctga cagagtcgct tcagataaat
    25441 ttaaatcgaa cttctcagcg gcttcggcat cctctaatcg aataaaataa tcgagcatat
    25501 cattatcgta tcccaattcg gattcggcat agcccccgat tccagttgct gagtaagata
    25561 tattggcttg aacagtggcg gcaatctcag ctggccaata gggaataata gtcagtttag
    25621 cataatcact tccgaaggtc tcatcggaaa aattataagt tccggtgcca ttacaggtga
    25681 cgaaggttat ttccggtgtc tcataatgtt taatcactgc caatccaaaa ctgtaagacg
    25741 attggtaatt catcacaaac tcgatatttt ggcctgaata aaactgaata taaaccgccg
    25801 ccgccggatt gagcgccaac gaaatctgct cgccttcggt aaaattagaa tgaaaatagt
    25861 tcgatgatat tggcgaaatc gccgccccac cataaccata ttgtccatca cccatcgtcg
    25921 gatcgttaag aaggttggca acaaaccaat cgcgtaatag ctccgcgacc gagccatcgt
    25981 aaccggcaga tgacaaacca ccgataaaac cgttaaatga gtcatttttg ttgttaaata
    26041 tctttttaat cacatctaat ccaaagcgct gatatatata ataagtccat aaaccgactt
    26101 tagcataatc ggctattgaa ccatcaaaat ccattaacga acgattggtc tcgtttaaaa
    26161 acgaggcaaa gctacgtgcc ggtaaaccca gcaagcgtgg cgccaattcc gagagccctt
    26221 ctgttaccca aacatcctca tctgaatcat aaaaattatg aatcaaatgc tgaagttcgt
    26281 gtgctacgat tgagagtgta cccagatcct tggcatccgc cggattagtg tcaatataaa
    26341 taatttcacc gtaattgcct ttgccaggca cctgatcgag cgggtcgaaa tagcccgcaa
    26401 cgtaggtatc ggattttccc acctcataat tatcacgtac gtcaatgagt aacacaaaga
    26461 gtttaccatt accgtctttg tccggtagtt cgccgaatat atcaatctcg ttgtaataaa
    26521 tacctttagc aggattaatc gaaccgggcg gcgtatcgta aagcattata tcgcgcaccc
    26581 gttcggcgtc gctttgagta attcgtccgg catcgaattc cgttttctca gcatatattt
    26641 caatttctgc ggtattaatc agacgaagga atgcaatttc aataaactca gccgaattat
    26701 cctcaacatt tttccgaata aagaaagtag tatctccggc aactggtttg gctaatctat
    26761 gatgaaaaac aatttccgga tatttattcc ataaaaatgg cgtacctgaa atcggttggg
    26821 gtgcaggagt atggagttcc tgcgccattg tgacagtcat taaacagcca gataaaagta
    26881 gtaatctttt tagtgaagaa atcaatattt agagagtgag tgccgctcct gtttaggatc
    26941 gcggttcatt aaatcccaca gagccgtttg cgggtcttta tttttaaaaa gaactttata
    27001 aatttcctcc gaaatcggca ttacgacttt gtacttctca gccatctggt gaaccgttaa
    27061 acaggtcgtg actccttcag ccaccatcga cataccattc agaatatcgt ccagtttgcg
    27121 gcctttgcca atttcttcac cgacgtagcg gttacggcta tgctgactgt tacaagtaac
    27181 aatcaaatcg ccgataccac ttaatccggc aaaagttact tctcgacccc cgactcgcaa
    27241 tccgagacgg acgatttcca taattccacg cgtcatcaga gcggctttgg cgttgtcacc
    27301 taatctcatt ccgtcgcaaa taccagccgc aatcgcaatg acgtttttga ccgccccgcc
    27361 gatttcaact ccgatgatat cggtattggt ataaacgcgg aaatatggtg aatgaaatac
    27421 ttcctgaact agctgggcag tctcttcatt tatcgaggcg gctacaaccg ccgtgggaac
    27481 ttgccgggca acttcttcgg catggctagg accatgcaga gtaactatct tttccggtgg
    27541 gtgatttaaa atttcagtaa ttaactcgct catacgcttg agtgtatttt gttcgatgcc
    27601 tttggtaaga ttaacgataa ttgtatcagg ttcaatctgc ccttttattt tctccagcag
    27661 ggaacgcatc gactgcgacg gcaccgccag caccaggaaa ttagcatttt tcacggcctc
    27721 agccagatca tttaaaatcg gaatcgcgcg cggtatcgga atgccctcca aaagcggatt
    27781 tcgacgagtc ttgttcatat acgctacatt ttccggaaaa tattcccaga gtttgaccgg
    27841 caatttttta ttataaagat ggatggcaat cgttgtcccc caactaccgg cgcccaatac
    27901 ggctatcgag atcgatttag gaatttttct tagcatgaaa atatattcag caaacctcac
    27961 cgagaataac cgctttttct ccggactttt gtaatacctg agaaaattta tcaacggctg
    28021 aagcggcgca aattataatc atcccgattc caaggttgaa aacgcggcgc atttcttcta
    28081 tatcaatttc accagttttt tgaatgaatt taaaaagcgg tggccattcc caggcttgcc
    28141 agttgatttt caaagtcaaa ttttcgggga caatgcggga agtattacca atgattccgc
    28201 caccagtaat atgactcaac ccatgtaaat ctggatcagc tagtaatggc tcaatcacat
    28261 ttaaatagga gcggtgaatt gccagcaagg cgtcgccgat ggtcgcattt aattccggaa
    28321 tgaaatcagt gacagaatat tttgtcaaga gcaccttgcg ggctaaagaa taaccgttgg
    28381 tgtgcaaacc agtggaaggt aaaccgagca gaacatcacc tttcccaact cgaccaatta
    28441 acatttgctc cctttccacc actccgatga tcgttcccac caaatcataa tctttggttt
    28501 gataaatatc agtcatctcc gccatttcac cgccaattaa ggcgcaacca ttggctttac
    28561 atgccagaga tattccccta ataatttcag tagttattgc tggttttagc tgaccagttc
    28621 caatataatc cagaaaaaac aaagggcgcg ccccagtcgt caaaatatca ttaacacaat
    28681 gatttaccag acattgcccg actgtattat gccgatcgag cagattggca actattaatt
    28741 ttgttccaac tccgtcggtg ctggctacca aaaccggttg acggtaattt tttaccggaa
    28801 aagcaaaaca gccgccgaat aagccaattt cagtcaatac attgggggta aaagtctgtt
    28861 ttaccagatt cttaatttct ttgacagctt gttcaccggc ggcaatgtca acgcccgcat
    28921 ctttatatga tacactcact taactttctg cctcctgata cattgattca attaaatccg
    28981 cataatgttt ttcaactacc gaacgtttta cttttaggga cggtgtcagt tccccgctct
    29041 cgattgtaaa ttctgatggt aatagaacat attttttaat tttctcgaat tgggcgaagc
    29101 cttccattga ttcggctatc actcggtcat acaattcctt gacagctgga agggcaatta
    29161 actcctcccg actgtgaaac tgcagattgt tctctttagc ccagtcttcc aaattaacaa
    29221 aagccggcac cactaaagcc gatacgaatt tccgtcggtc gccgatgact acaatttgtt
    29281 cgatccactt tgaggtaacg agcgcatttt ccaccggttg gggagcaaca ttcttacctc
    29341 cggaagtcac taatatgttt ttcttacgat cggtaatggt gatatagcca tccccgtcaa
    29401 tcattccaat atcgccggta tgaaaccaac cgtccgcatc gaggacatca cgggtagagg
    29461 cttcatcttt ataatagccc agcataacgt gtgggccgcg cgtcaggatt tcgccatccg
    29521 aagcgatttt cacctcgata ttagggagtt tcggaccaac tgtcccgacc ttacattttg
    29581 acagcggatt aacggaaata actggtgatg tttccgttaa accgtagcct tcgagaatcg
    29641 gaattccagc ggcttggaaa aattgggcaa tttccttaga gagaggtgct ccgccggaaa
    29701 caaaaaaccg caaattgccg ccgattttct catataattt tgaaaaaacc agtttttgag
    29761 cgatttttag ctggttatcg agaattgcgc cagtgttccg attctcactc tttagaaaaa
    29821 cggctttttt accgacattt attgcccacc agaagatttt ctgactaatt ttggaagatg
    29881 ttgaaatact gtcaataact ctagcgtaaa ccttctcgta aaaacgcgga acggcgacca
    29941 tcaatgtggg atggacttcc agcatattct cggcaaccgt gtcaatactc tcagcataac
    30001 tgatacatgc cccggaaccg gtggctaaaa aatgtcccgc catacgttca aaaccatgac
    30061 tgagtggtaa aaacgacaaa aaagtgtcat tttcattagc ctcaacgatt tgtaatgcac
    30121 cggtaatatt cgaagtgaga ttgccatgcg tcagcatagc acctttgggt tcgccagtcg
    30181 taccagaagt ataaattata gttagcaaat cgtcttcctt ggtattatga atatcttcaa
    30241 ggaaagcact cggattctgg cctcgatatt cggcacccat ttgcatcaat tcactcatca
    30301 taatagcttt cgggtgatca attttcgtat catccattat aatgacttta taaacttcgg
    30361 gaagttcgtt aagaagtgga atgacttttt cggtctgttc gcggtcacca caaaaaatga
    30421 aacgagaatc agaatgccgg gcaatccacc acacttgttt agccgtaaga gttgggtaaa
    30481 tcgtcacgac tacccctttg gcacagatca caccataatc agtcatcgcc cataatggat
    30541 tgttagccgc aataatggcg acatgttcgc cagctttcat acccaaggct ttcagtccgc
    30601 cggaaatttg ctcgacacgg ttgcgcactt ccgaaaaagt aagtcccagc catttcccat
    30661 cctttttata tttgtacatt ggccgatcag tgtatttatc cgttatgccc acgaacattt
    30721 cgctaatagt actatagcta aaatccgaca taatcaactc cctatattta cttttttatt
    30781 gtttttaact tgtaaagagt taattttagg gcgctttttg ccgaggtggt gaaataggta
    30841 gacgcagtgg actcaaaatc caccggactt cggtccatgg gggttcgatt ccccctctcg
    30901 gcacaaaaac cacaatcata tatcaaagca actccgcatc aatgtaatat ttggattcat
    30961 tgatttcact ttaatagctg ataaaaaaat atttatttaa tcttggttaa cgtcgccgga
    31021 ataaatctga attgcttttt tggctttggt tacttcctca attgcattcg gataatctcg
    31081 tataactttt tcccaggcac ttacagccag atctacctga ttcattcggt tatagcaata
    31141 gcccatacgc agttgagcat aagcagattt attcccatca cccagcccaa atactttgcg
    31201 atatgcattt aaggcgctga cataatcgcc aagaaaataa tagcattcac cgatccacca
    31261 ctgcgcatta tcagccagcg gatgttgcgg atctagattg ataaattcag tataaacgct
    31321 gatggcatct tcgtattttt tctgttgaaa tagatcgtta ccagtttcat aaagcatatc
    31381 ccgagttctc gggcggcttt ttggtgtttt tgccttttct ctttgaacag gttttggcgc
    31441 tgtactggtt acctctggtt ctggtgcttt tccatgaaac acaaaatccg ctatagttgg
    31501 gttgaccact tcaccaacga ataattcaat tgacacccgg tgattcagca gttcttttac
    31561 ttggttatca ctctcgatca gcatttcatc agcaccaaca ggcacgattt caatccggtt
    31621 ggcaacgcta tccttctctt cttctggaaa taaatcaagt attaatttcc ccactgtaat
    31681 cgcccttttc tgagatttta tgatagcatt accggatttc gtaccagtat ccattgcata
    31741 gcctttcaac cgaattttga tatccggatc actcttcaga gctttcacta ctttgttaag
    31801 agtaactata taattggagt taaatgtaag gttaggagtt tctgtttcat cggcatcaaa
    31861 aaatatatca tcaaaaataa ggatttttaa aaccttagcc gaagtttcgg cttcttttgt
    31921 acataaaccc ggtttttccg taacaacctt actttcgagt actccatttg tctcattgga
    31981 agtcggtagc aaatcagcat ctgttttgat ttcatccgtt ataacgggag agggttgtat
    32041 agctagctcg gaagtattac cagtcagagg agttgaatcc ggctcatttt gaacatcctt
    32101 tttggctgga gtaacagaat ccaattgaga ctctttcacg gcggttactg attgcataac
    32161 cattccagct gtatcttttc taaccgtttc aaggttagta gtctcggttg aagtttttgg
    32221 actttgtacc ttttttggtt ctgttttttc agttactttg gtttgttcag tccttctttc
    32281 tactttctta gtttcttttc caccagaaca agcaggcaat gctaatgtgg tggtaaaagc
    32341 tattaaaata ataaataaat attttttcac ctctccctcc tctaagattt taatgcttaa
    32401 caaatccaat tatccccaga tgataaaaat atattattta tctatcagat tatttttttg
    32461 ctggggtagg tggagtttcc aaggtcttta cacgtttgtc gagatcactc aattcacgac
    32521 tcatccgggt aaccgcggat tgtagatcag taagggtccg ctgagtttca cgttgaaatt
    32581 tattgagtct ttcgttaatt ccatcctggt cggttttcat aacattaaaa cgagaatcga
    32641 tatcaatttt taattcagct atgctggtgt tagtttcttc gattttgtca cttagcgcca
    32701 agtccacgct gtctaatcgt gaattcatat ctttcagcga ttgctgaaac tgttgaatat
    32761 gtttgcctct cccgacaaat tccagactca gatcatgtaa attatgatca aaatcgttga
    32821 tggttacact atgggtggaa tcacgggcat ttaaaatgtc caggcgtttg tttacttttt
    32881 caaattcaaa gaaaaaataa acgaccgcta caactacaag caccaatgct atggttttaa
    32941 ttagttcttt cattcatgtc tcctgtacct ttacttattg ttaatatttt aaccgaggtg
    33001 tttagcccag tcattatctt catttgccga aaaagggtta ggttcagctg gcgtttgagg
    33061 tatatcagat attcccataa tcggttgatc agtactgccc gtagaatttg acgattttag
    33121 ttctttaatt agattctcga cgtcttgatg aaaaatagca atggtttttt ccattctatg
    33181 aaataactct tccattaact ctttgccata gaggttattg ataccttgaa ataagtcgtc
    33241 aaatttagtt ataagaaaat ttttacggct ttcctcaatg ctaaaatcga cttttttatc
    33301 ttccataatt aacgctgtat tctttttaaa atatcaatat ttaatttaca aataccattt
    33361 taattatcaa tatttttcta tgttcagact accacctttc ttgctttagg tcttcccagc
    33421 gatcaatttc tcggattatt tgacgacaaa cccctcgaat aacactattt aatttaatct
    33481 cgactgaagg ttgattagaa gtcaactgtt gtaagacttg acggaattcc agtagttcac
    33541 tcctctccag ctcaattgtc atacgggtta acatttgatt tcactccttc ttatttgaat
    33601 ttaccaattc taccatatta tgggaatttt ttgtccacat tcacaacgat taccttgtag
    33661 agactttgtt acttgagtgt accatccccg tttgatcaaa aggcgaccgc aagccgggca
    33721 aaaggtatcc gattctgaat cagtaagaac attaccttca tagacgtaat gcaagccagt
    33781 gcgcagtgct attgttcgcg cccggtcaag aacttcttta gttgtctgag gccaattgct
    33841 cattttgtag gctgggtgaa aagccgaaaa atgcaacggc gtatcatttc ccaggttatc
    33901 aagaatccaa tcacataact tttgtatttc gccttcgcta tcgttcaatc cgggaatcaa
    33961 gagagttgtt aactctatga aagttccgac cttctgaatg gcaacgattg cttccaaaac
    34021 cggtgtgagt tccgcatgac agcgtttgcg ataaaaatcg gcgttcatag atttcaggtc
    34081 aatattagcg gcatcaatca gcggataaat gtctgcaatt gcctcctggg taatgtaccc
    34141 attagttacc ttaacagtct tgagattgga ttggtgagct attttggcaa tatcaacaac
    34201 gtactcgcca aaaatcgtcg gctcgttata ggtaaaagca atcgagccac attgattatc
    34261 aattgcagac tgaacgattt cagctggttg cacaattcga cccatcccaa gatggccatc
    34321 cgcggtagaa atgtcccaat tctgacagaa ttcgcattgc agattacatc cggctgtgcc
    34381 aatggaaaaa attcgactcc ccggttgaaa atgatatagc ggtttttttt cgattggatc
    34441 aatatgtatg gcaattggtt taccatacac ttctgaaaag agacgccctt cgatatttgt
    34501 ccggacgcgg catactcccc ttttgccgtt tttcaggcgg caatgtcgtg gacaaagaaa
    34561 acattctacc tcattatttt ccagggcgtg ccaccagcgg gcaggatata agttttttgg
    34621 tagttctaat ttttccacat taaaaactac acaattaacg ttcatttgtc aacagtagat
    34681 ttgagaaaaa ttttctatta atgattaaaa cattttacaa tgagataaat tttcgactga
    34741 ggagaagttt tacaagttgc aaaatctcaa acaatcatat aaatttaaat tactatggca
    34801 caagtaaaga caacaaaaaa agtaaagacc aaagaaaagt cggcaagctt cataaaagct
    34861 ctctctttta caaagattaa ttatctactc tttgcaattg gaattgcagt aataataatc
    34921 ggttatattt gtatggctac cggtgaaaca tatagtttcc gttcgctaac cgtagcacct
    34981 atcatacttt taatcggcta tttgataatt attcccctgg caattcttta tcgcaaaaaa
    35041 gaagaaaaaa cgccctcaga gagctgaccg aaattattaa aatcaagcgc tgatttcagc
    35101 tgtttatgaa tataaattca gttggaacta ttagatgatg tcgttattgt tttttcttta
    35161 gctttgggtg tggaagtagg tttgtcttct ggagtggttg gagatgggct tttagtcttg
    35221 tttttgtcac cataatcatt cacataaaaa ccggagcctt tgaatattaa tccggcgcca
    35281 ccactaatct tccttgtgac acttttttgt ccacatttag ggcagacttt tagtggttcg
    35341 gcactcattt tttgaaattg ttcaaattca taaccacatt tttcacactt atactcataa
    35401 gtaggcatat atttttctga tcctatattt aaacgagatt acagataatt gctgtcacat
    35461 taacgacatc ttcaacgcta catcctcgag aaagatcatt catcggtttt tttaatccct
    35521 gaattatcgg accgattgcc tcgcaattac cgatgcgttg aactaactta tacccgatat
    35581 tgccagcatt aagatccggg aagattagta catttgcctg accggcaaca ggactaccag
    35641 gtgctttttt agctccgatt gtgggtacga tagccgcatc ggcttgtaac tcaccatcaa
    35701 ttctcaaatc gggatatttc cctcgcacta ttgcggtagc ctgaatgacc ttatctacta
    35761 gctcgtgttt ggcactgcct ttagtagaaa aagaaagcat agcaacaatc ggctcggttt
    35821 ttacgactct gcgatgagta ttcgcggtcg caatggctat gtcagccagc tgctcggcag
    35881 ttggattggg cagcacagca cagtcggcaa aactccagat tctttcttca tcaggtgtta
    35941 taatgagaat atcactggaa actgttggag tatccggatt agttccgatg atatgcaatg
    36001 cggctttaag aacatcgcca gtggttgtat cggctcccga aacacagccg tccgctaatc
    36061 ctttttccac cagcatagct ccaaaaaaag tcgggtttaa cattacggtg cgagcatttt
    36121 caagggtcat ccctttatgc ttgcgttttt cataataagt attaataaaa tcgtccaatt
    36181 tttcaaattg aacagggtca aatataccta tcgagccgag attgacgtta atttcacggg
    36241 tgtgttgacc gattatctct ggatcaccga ctaagattat ttgcgctaag ccttcttttt
    36301 ccaaaatttc ggcggctttt aaaatacgtt catcatgtgc ttccggtaaa acaaccgttt
    36361 ttttacactt ttgggcttga gacctaattt cctcgataag tttcatttta ctccctgaat
    36421 tttttcaaca gagccgcctt cacatttgcc ggcacaaaac tacctatttc tgcaccaaat
    36481 ttggctaaat ctcgcaccat agaggaactt agataagtat aatcttcatg gggcattaga
    36541 aataccgtgt taatattttc atccagatga cgattcatta gcgccatctg aaattcataa
    36601 tcgaaatcag ataatgctcg taagccgcgg atgattacct ctgccccgac ttgacgcgca
    36661 tattcgacta ctaatccatc aaagctatcc attttgacat tctcgaattc gctcgtacag
    36721 cctcgaatca atgccagcct ttcttccgtg gtaaaaatcg gttgcttctg gagattgcga
    36781 gctacggcaa tatatactaa gtcaaataaa gatgacgctc gtttgagtat atccagatga
    36841 ccattggtaa taggatcaaa actaccggga taaactgcta ttttcattta tatccttatg
    36901 aaattcaaat tagtatcgcc acttctgcga atttttattt cccgcggttt aaaagatgaa
    36961 ggcaggtcaa atctacgatc cgtttcgagt acaatttgag tatcgggtcg taattggccg
    37021 aacagcatta atagttcatt ataactcgaa tattcatatg gtggatccgc aaaaatcagg
    37081 tcaaaaagcg gcttttgggc taaatatttc aggacatcag cctgaataac ttcagcccgc
    37141 tgttccaatt tgagttttgc taaattgtgt ttaaccaaat ttaggctttg ccaattatta
    37201 tcaacgaaag tgcatcgctc ggcgccgcgg gatagcgcct caattcctaa attaccggtt
    37261 ccagcaaaca aatcggcgac ttttagaccg gatacatccc ctaaaattga aaataaagcc
    37321 gcttttactc gatcctgagt tggtcgaatt gtcgcttttt tatcggacat tatggattgg
    37381 tttttataca aaccggcaat tacccgaacc atctttacaa tttttgtgga tagattacag
    37441 aattaatact gaaatcgagt attccggtat ctactgccga atcagcactt aagtttattg
    37501 caattttttc aacacttaaa ttcaaatcaa aattcgataa ctcacttaat aaattaataa
    37561 tactgttcag tgagccattc ccacgaattt ccgccaaacg gggctttggc gctggtggtt
    37621 cggcaatatt aagcggctgc aactttaaat tttgacgctt accgattgcc caaagcactt
    37681 gcgagagtga tacgtcatca taaaaacgga tatttctagc aactgcactc tcagtcttta
    37741 aattgagaat tgccatcatt tggacagcgg gcgaaggagt gtccgcaaca tgttcaatat
    37801 aaaatgcttg aattgatcca attcccggta atttttgcac ttcgtcttta acctgctgag
    37861 cttcattctc agtatcaaca aaaattaaga aattgaccac atctttatct aattttaaaa
    37921 attgaattcg tgaattgtct ggtaaaacac taattaactg ggataatatt tgacaacgag
    37981 atataccagt taaaacaggg ggcagatata tttcatcaat ttgatttatc gggattaaaa
    38041 ccggctgatc aacttctggc atatcggagg tagaagaagg ctgggattgt tccgttaaaa
    38101 gaattggctg ttcaaccggt agatccgact ttccggttgg tttagcggta attgccgatt
    38161 ttcgtggatt aaagtactta gtatatcccc aataacctaa agctccaact ataaccagaa
    38221 ccaatatcca gattccgata ttaagaataa ggcggttaga taaatgcctg gaatattcct
    38281 cttcttttct ttcgacgatt tgttcggctt ctctcagctt atcggaaacc ttaggtgacg
    38341 gttgagagat ggatttttta gcgtcggttt ctcccggcat acttgaattt gaaccttgtg
    38401 aagcaaaagc atccagcttt ttctcaatat tttcaggtat cggtttagtc gaagatttgc
    38461 taattgcttg atcgtcattc ggttgatttt tcggctcgac cgccattttg attagaatat
    38521 ctttaaggct ctgactaatc gcaatatctt taggttcttg ttgtagtcca ggtgtagcgg
    38581 caagattaat ttttatcata attcgaccta aagcataaac cgatataaca atcccattac
    38641 atccaagaac tgactcatag ctccgggacc cgatccatca tcttggtaaa ttttaaccgg
    38701 cttgaaattc tcaaaaggat caacacatcg tagattatca atatcataag tcaaaagcat
    38761 attataaaat tcagaattga catcataagt gaaaaaataa aggttttcaa tatcttgaat
    38821 cgtttcactg cttaaattcc taagattatt tatttgagaa acgatctctt tcccatcttc
    38881 gtcgggattt gaatccacca atatgttata atccccatcg ttatttaaat caaattctaa
    38941 atattgactg atttcacccg atttgataag taggagaata tgatattttt cacctattag
    39001 ccataagccc cacttttttc catttttttc tttatagaga ctttcaagcg cgttagccgc
    39061 gctaaagata ttcagatcaa agacttttat gataaatccc aaccgctgac aggcttggta
    39121 aatagcccgt cctaattcct taaaatatga aattgttaaa taatcacgct tattttcaga
    39181 aattcgttgt ttcagaggat aatgctgaac aaattttttg ttaaatacta ccccaagacg
    39241 ttgtttctcg ttccagtcca gaatctcggc aattttctgc ttttccaatc ccagatcaat
    39301 tgaattgatc gtaatatcaa ataattgcga gggagctgat aaagctaaaa accgttcctg
    39361 cacttgtaac ctattcttga tattaagcaa aagggattca atgctaggaa tcagatcagg
    39421 attttttaag gaagtagtat caaatttaaa cggcagtttt tttttaccga tcaattcgat
    39481 cgatatagaa ttgtccgttt taatcagctg gccataccta ataaaattat ccgaaagact
    39541 tattgccagc atcttatctc cgaattgttt ggctatttac ttccaactcg gttcaccgtt
    39601 ctcaatatac ccgtgtgatg aatctacaaa agtaaaaaag aggtagcgcg gttcacggat
    39661 aacagttttg agagggtctt ccaccctaat tcctttttcg gttttaagaa tagtgtaagg
    39721 agtatccaat agagggcgca tagctaagtc ctcagttaga taatatctac ggtaataagt
    39781 attattagct acccgttgcg tgagttcggt atcaattaca cttctgaaat tgggatcggt
    39841 ttttacttct tcaagcattg aggagattac atagagtgta tcaatggtca taagttccgg
    39901 attccactta gtaattttaa tagtagtatc aatgaccgta tcggacaact catatttaaa
    39961 agcaaaatta gtatcgaaag aggcgtaaaa gttcttcggt acttccaaat tgaatttgcc
    40021 atccggtagt ttgatcactt tttgaccata aaaattgctg tcagcccggg tgctatcccg
    40081 aaccgcgctg actaccttca tagctagaac cgggtctggt tgatgagttc cagccatctg
    40141 gttataatat ttctggacat catactgaat ctgcattctt tgacgaccta attcccgaat
    40201 tcggttctct tctttccaga tcgaactcgg aatataaatt acggcaatca aaacaatgat
    40261 gagaatgaaa gccacaacat ttaatacttt cactagtgtc ggtttgtatt ccgggattat
    40321 ctcttcttcc tggttgaatt ttgcagtttc gtcactcaag ggttacctct cctttaattt
    40381 tttcaaacgt tttagttccg atacctttta catttaggag ttcttcaatc ctgttgaatt
    40441 ttccaacctg agcacgatat tccaaaatat ggtcggcagt taccgaaccg atttttggca
    40501 ataacatgag ctcctgtttg gttgcggtat ttaaatttat cttttgttga agagcagttg
    40561 atttttgtga ttgatatgta gaatgaccgc ttctctgtaa ctcggtatat tccgaatcaa
    40621 tttgactggc atatagttga aaagaatcag taactacagt ctgttggcgg cttttttcgt
    40681 tattaatccg gttcgacctc attttataga tacggatagt tgcacctacc aggaaacaag
    40741 caactaataa ctgaataacc attttctcct ggcgggttaa atttatcatt tttcctaaaa
    40801 attaaaaatc ttaaaaaatc tcattttccc ttaatgtaat attaattagc gaatcttact
    40861 ataacgtttt tcatctttta ttttatttga ttcgaatttg aataattcgc ggtataattc
    40921 caattctttg ccactgactc tttcaggaat tttaacctga attctcacca tcatatcgcc
    40981 tctgccggga tgttgtaaat gtggtaagcc tttattctta agtcgcagta ttttggacgg
    41041 ttgagtgcct ggcggaatag tcaggcttac tttaccgcta agggttggga cgacaatttc
    41101 cgtccctaaa actgcctccg atggcataat gtgaagatca aggtaaatat catctccttc
    41161 gcgagtgaaa tattcatgcg gtttttcttc gaaaattaca attaggtctc cgcgcgaact
    41221 tttgcgaacg ccagcattac cctcaccgtg catcgttaaa taattaccag tagtaactcc
    41281 cggtggaact ttaatcgtca gttctttaac actctggata cgtccttcgc ccccgcactg
    41341 aggacaacgc tgttcaacaa ttactccttc gccttggcaa ttagaacaag gacgcacatt
    41401 taccatttgt ccaaaaaacg aacgggaaac ctcccgaact tcgccacttc cctgacaaac
    41461 cggacaggtg acatttgaag aaccgggttt agctccggta ccgttgcaat tggggcagat
    41521 ttcttgacgt tttatcttaa cttttttatc aacgccggta ttgatttctt ctaaagtaag
    41581 tggcattgta attttaaggt cactaccgcg ttgctgagtc gtacgagttc gagtacgggt
    41641 agagcgttgt cgcccaccgc cattgaataa gtcatcaaat ccgccgaaac caccgaaatg
    41701 gccaccaaaa atatccccga acatggaaaa aatatcttcc atattcatac tctgcgcccc
    41761 gctaaaacca gtcgtacctt ttagtccgtc ttcaccgtat tgatcatata cccgtcgctt
    41821 ttctggatca gatagaacgg cataagcttc ggcggcttct ttaaatttct cttcggcttc
    41881 tttattccct ggattgcgat cgggatgata tttcatagca attttccggt aggctttctt
    41941 taattcatca gcctgtgcat tccggttaac gcctaaaatt tcatagtaat cttttgccat
    42001 attatttact tacaaccact ttggcgtatc ttaaaacacg gtttttaaat ttataaccct
    42061 tctcatattc ttccagaaca atatctggcg ctactcctgc ttcttcacgg gtcataatcg
    42121 catggtgcag attgggatca aatggtttat tgaccgactc aatcggttct acacctaaat
    42181 tctgcaaggt tttttgaaat ttacgataaa taagttccag accttcacgc aaagattgat
    42241 catcgttgat gttatttaaa gcccgctcta aatcatccat aatcggcaga atgtttcgca
    42301 aaacttcttc accagcgtac tcgattatat ccgaatattc cttctcacgt cttttacgat
    42361 aattatcgaa ttccgccgct aaccgcaaaa atttttcgtt agcttcgttc aaggcttttt
    42421 catattcagc tacttttgct tccagttccg cgattttggc ggataatctt tccttttcac
    42481 tggtttgctt tttagttgac cgagtctgat ttttatttat accagacgtt ttttcattta
    42541 tacttatatc ttccacgatt cattctcctc aaaattaatt tttaatcatt taaataaaac
    42601 tcgttctgaa ttatgcaatt ccaataccag ttataagaag ttgactattc gctcagtcaa
    42661 aaatgcgatt attcggcttg gagaatcatt ttttcggact ttctgacaat tacagttttt
    42721 actcgggcgc ttattgtatt tttctatcat cataaaatca ttcataactg cggaatttaa
    42781 attttctgat aggtcattcc aaaataaatt gagtaatatt atttataatt taaagggaat
    42841 tattctcagg tatttcttat tcgaggaatc tacgaatagc gcgcatacta ccgattaaac
    42901 cgattaaact gcccaaaacc agcaatccta tataataacg gtagctgacg attatttgat
    42961 aaccagtaag tccttgtaaa taattattga aaaaatagga gatcagaccg attacgcctg
    43021 aagctaataa agccccgatc aatccctcga gcgttccttc aatcaaaaaa ggcgctcgta
    43081 cgaaattatt cgttgctccg actaatttca tcgtagcaat gacatttcgg cgggaaaaaa
    43141 tcgtcatttt aatggaattt gaagctaaaa taatcgagat caacgtcaag ccaataaaag
    43201 ctgtgactac ggcaatgaat atcaagcgct gatacttttc cagcaatcgc aggaaatttt
    43261 cacgatagcg gacttcatcc acgatcggat attgtttaat acgagtagta agatcctcaa
    43321 tactagctag attacgggct tgaggcttga gacgaagagt aaatgaaggt ggcagggggt
    43381 tataatcaag aatactaaat atatcttctc caaattcggc tttgaattgg gcagcggcac
    43441 tatcggttga aatgtagtca attttttcaa tctctttaaa gtaacttaat tcctcaataa
    43501 aattttccaa gtcggttaat gacgcattac ggtgaacgaa aatagttata tcgaactgtg
    43561 aacgaatatt ttccaccatt actaaagtat cacgggcggc aacgtatccg aatccaatta
    43621 gaattagcga tagagcgata attatgatgg aaaaagcacc ggcatagccg gaacgcccta
    43681 atccgcgaaa tccctcttta atcaaaaacc acaatatggt catactggat taattatttc
    43741 tccttcgttg acctgaatta tgcgcgcatt cgccacccgc gggatgagtg aataattatg
    43801 cgtagcaacg attacggctg tcccagtttg gttaatttta attagaagat tcatcaaatc
    43861 ggaagcgacg cgcggatcga ggttgccggt cggttcgtcg gccaataata ctaccggttc
    43921 ttttaccagt gctcgggcaa tggcgacccg ctgttgctcg ccaccggata attcgcgggg
    43981 attatagtaa acgcgatgtc ccagcccaac ttgattcaaa acgttataga ctcgattttt
    44041 aatttcgcga gttttataat gtgacacata cagactaaga gccacatttt cataaacgtt
    44101 ccgatcctcg agtagtcgaa aatcctggaa aatcatcccg actttgcgtc ttagaaatgg
    44161 gatttcattt ggtcggactt tagaactatc aaaattacgg atgacaactt tgcccttttc
    44221 gggaaacaag tccatatata tcaagcgtaa aagcgttgat ttcccagcgc cggaagaacc
    44281 agttagaaaa acaaactctc cttcattaat cgtaagattg acatctttaa ctccggcatc
    44341 tttaccgaag gtgcaactaa cgttgaaaaa agttatcata atttaagttc taaaactcgg
    44401 acaatttaaa cgcctgaata taataaatca acgccgtttc ttctaaatat tatagttata
    44461 cttcatagtt actttaactc accgaaaaac tttctcttga aatctaaagt cagcttgaat
    44521 aatttgccaa taggaaaatg gagaaatgtc aatgaataaa ttaagatatt ggatcattac
    44581 cttgattttg gatgtttggt gtttttcagc gaccggtcct tgctttgatg actattttct
    44641 tgaccaaact atgcgactcg atttttactt cactggaacc aagactcaga acattttcag
    44701 tctcgatgcg gtttatatta gtcctctctg gtcgggcagt cagaccaatc tgattgacac
    44761 gctgaaccta gggcaccatc tcattcgcgt ttacgatgtg gacagccaaa ctttgatcta
    44821 ctcccgcggc tttagcagta tttttaatga atggcaatca actaatgaag ctattcagga
    44881 aatttggcgc acttttcatt tttctgtcct gattccatta ccgcgccaag cgatcaccgt
    44941 aacgctttct tcccgtgatc gccaaaatca gttcatcgag tattttaaga ccaacattga
    45001 gcccgattcc cggtttatta atcgggatgg cgtccatact gattatcggt tgaaaaagtt
    45061 aatctataac ggcgcaccca cccaaaaaat tgacctgcta attttgccag aaggttatac
    45121 aaagtctgaa atgcgtcaat ttcgccagaa atgtcgtcat tttaccgagg tcttattcaa
    45181 cgtgagtccc tttaaagagg aatccgataa atttaacgtc tggttcttgg aagtgccttc
    45241 agctgaatcg ggcattgata atccgcgagg tggtaccttt aaaagaaccg cctttgacct
    45301 gacttataat gctcttgatc tcgatcgtta tgtattgaca ctcaataata aaaccgtacg
    45361 tgacattgcc gctcaggctc cttatgattt tatcattttt atttttaatt ctgataaata
    45421 cggcggcggc ggcattttta atctttattc tacctgctat agtaccgccg ccaaaccgga
    45481 acaaaaaggc tggcctgagt atgttttcgt ccacgaattt ggccattcat tcgccggtct
    45541 ggcggacgaa tattacacct cgagtacgcc ttataatgaa ctctacccga tgggagtcga
    45601 gccatgggaa ccgaacatta ccgccctatt gaatccaacc acattgaaat ggttagattt
    45661 ggtttctcct gaagtccctg tgccaactcc ctgggaaaaa tccgcttatg aacaagccac
    45721 ttatagcaat gcaagtgaat ttgaacaatt tctaaaaagc caaaaatatt atggaaaaat
    45781 tggggcattc cagggtgctg gttacgcctc cgaaggttta tatcgtccct gcctggactg
    45841 cattatgttt tcgcgtaatg aaatcgcctt ttgccccgtt tgtcaagcgg caatccggaa
    45901 aataatccat ttctatagcg agtaaagtaa tttcaatata attaaattat tgtaaagaca
    45961 tcaaaatgta aaataaatga tgaaataaca cgctaacgat gccgaactta aattctatag
    46021 aaataaaaaa acgggctatt gaactttata aactgatcga tccctgtcaa atttgcccgc
    46081 gactctgccg ggcaaagcgt ttacacggag cggcgggatt ttgtcaggtg ctggataagg
    46141 ctattctttc cagcgctctg gcacatttcg gcgaggaacc accaataacc ggtcgcggcg
    46201 gctctgggac aatctttcta tcatcctgca acgccgcctg tattttttgc caaaattacc
    46261 agattagcca cgaacggatc ggcaatcccg tatctatttc tcaattagct aatgaatatt
    46321 tacatctcca ggctgtcggc tgtgaaaata tcaactgggt tacgccgact cctcatttgc
    46381 catttctggt ggatgcactg gcttcggcag ttgaacaggg attaaaaatt ccagtcgttt
    46441 acaataccaa tggttacgac cgggtcgaag tgttacgtct tctggacggt atcgttgata
    46501 tatacttgcc agatatgaaa tacggcgagg atgtctgggc agaagaatat tctggactaa
    46561 aagactattt cacaataaat gtcgcggcag tacgcgaaat gtaccgacag gttggaccat
    46621 tgatcataaa tgaaaatggc gtggcagtca aaggtttgct ggtgcgtcat ctggtcttgc
    46681 cgtatggtcg cgccggtagc gctcgacttt tccgcgcctt agcgaaaatt gaccctcaag
    46741 taccggtaag tctaatggcg caatatcatc cttgttatca agccgtcggt cacccggttc
    46801 ttggtcgccg aatcttccgt cgtgaatatt taaaagcctt agaagatttc gaaaacgccg
    46861 gattattaaa tgcctttact caggactata ttagtctgga acacgaggat tttttcttcc
    46921 cggattttaa tgataacgag cggattttta attgtcgtga taatcgtaaa aaatgagatg
    46981 ccgttttacc agtctattaa atcctataat tcaactcatg agaattagat cgctttcgaa
    47041 aaaatggctc tgtaatacgg aaagaaattt gatctacacc gctgtctcat ttactcaaag
    47101 taaatccaga aatgcctaaa ttatcctaat attcggtaat agtcctgacc cagatttggt
    47161 tccctttcag atattatttg cactgcccac ttacacctga aactgaccat atgatcaatg
    47221 ctaaggcgct tgcactgatg aaaaacggtg tgatgatcat caataccggt cgaggacgat
    47281 taatacagac agaggattta atcgaagctt taaaaagtgg taaggtaggt tcagccggtc
    47341 tggacgttta cgaagaagaa agcgagtatt tcttcgagga tttctcaaac caggtaattt
    47401 ctgacgatat gctggctcgc ctcttaatcc tgcctaacac gcttataact tcgcatcagg
    47461 gatttttcac cgctgaagcc ctccagaaca ttgcagaaac taccctatct aatattaccg
    47521 aatattttga aggcgactac ctaaaaaacg aagtctgcta taaatgtgat cacgattgcc
    47581 ttaaaaaaca aaaaaagaga tgctggtaaa ttgtaataga tatctcaatt ttttcccgat
    47641 aaaatcagga ttttcctaaa atttcctata attatctatt aatttgccga taatccaggc
    47701 aatatcgtaa taaacaatta gccaaggctg atattcaata tatttatgaa aatcctgcta
    47761 catatcaggg aaaaagtgga taacatatct tgatactata taggtttatt gaacaataat
    47821 tcttccgaat cgctgattat ttggttaaat ttcttagcta tttttagtat tattaggaaa
    47881 caataaatgg ataatatcga aatacaaaga attagaaata ttggaattgt agcccacata
    47941 gatgctggta aaactacgac ttcagagcgt atcctctttt ttaccggtat gactcacaaa
    48001 atcggcgaag tccataatgg tcaagctgtt atggatttta tgaagcagga acaagaacgc
    48061 ggcatcacga tcacttcggc ggcgatcacc accgaatgga aagatcatca aatcaacctc
    48121 atcgatacgc ccggtcatat cgatttcaca gtagaagtcg aacgctcttt gcgtgttctg
    48181 gatgggatag ttatggtttt ttgtgcagta ggcggcgttg aaccgcagag tgaaaccgtc
    48241 tggcaccagg ctgacaacta caaagttccg cgcatcgcgt ttatcaataa aatggatcgc
    48301 gccggagccg attattttca gactattaat atgatgaacg aaatgttggg tgctaatgcg
    48361 gtcgctttcc aattgccaat tggcgacgaa gccgatttta atggaatcat tgacttaatt
    48421 acaatgaaag cctattcata tcgcgaactt gatatgttcg aaagcgaaat cccatctaat
    48481 ttattgccga aagcccaaaa attacgcgag cagatgattg aaaaattggc tgatttcgac
    48541 gaaaacctga tggagaagtt tatcaatggt atggcaatca gtgagtccga cattcgtaat
    48601 gccgctcgtt atgccgtctt aaatttatgt gtaacaccgg ttttctgtgg ctcggctttc
    48661 aaaaataaag gtattcgctt actacttgac gccatagttg attacctgcc gtcccctgtt
    48721 gatcggggaa taattaccgg acgtgatgct aataatcctg aagttatcat ttcacgtaaa
    48781 ccccatattt cagaaccgct gacggcgttg gcattcaaaa tcactaatga tgcttacgtg
    48841 ggtcaacaga ctttcgtgcg ggtttactcc ggagaattac attccggttc ttatatcttc
    48901 aattctacca agcaaaagcg ggaacgtgtc ggacgggttc tgcgtattcg cgccgatgaa
    48961 cgtacagaaa ttacatcttt gaaagccggt gatattggcg ctttaatcgg tatgaaactg
    49021 acaactacag gtgatacgct ttgtaacgag aatgatacta ttttactcga aaatattcgt
    49081 tatcccgaaa ccgtactgga tatgaaaatc gaaccactcg caatcaaaga acgagatcgt
    49141 cttggaatag ccttgagtcg cttggctctt gaagacccct catttaaagt gcgatttgcc
    49201 gacgaaactg aagaaactat catctccgga atgggagaat tacatctcga aatcatcgtg
    49261 gataggttaa agaacgaaca caaactcgac gtcaatgtcg gtaaaccggc ggtagctttt
    49321 cgcgaaacta ttacacgtga agctgaacat acttatcgct ataaaaaaca aactggcggc
    49381 aaaggtcaat ttgctcagat tgctttccgt ctcgagccaa atccaggaaa gggtattgag
    49441 tttgtcgatc gtgtcaaggg tggtaacatt ccgcgcgaat tcatcccggc ggtcgaaaag
    49501 ggttttcgcg agatcgtcga aaaaggttta ttagccggtt acccgatggt agatattaaa
    49561 ttcgttcttt tcgacggcgc ttatcacccg gtggattcca gcgaaatggc tttccgaatg
    49621 tgcacgatcc tggggatgaa ggaaatcatt aataaaacgg caccgcaatt attggaacct
    49681 attatgaaaa tcgaaatcaa tacgccggat gaatatatgg gcgatgctat cggtgatatc
    49741 aaccgccggc gcggtaaaat tgaaaatatg cgccgtcacc gcaaaggctc acaaaaactc
    49801 aatggcacag tcccattaat ggaaatgttc ggttatgcct cggctttacg cactatttcc
    49861 agcggccgtg cagctttttc gatggaattc ttacattata ctcccttacc aaaaagtatt
    49921 gaagaaagta ttattgaaga acgtcgcaaa aagaaagccg aaagagatta atttgttaga
    49981 aaaaacgata aaaaagcccc gactatcttt ttccttaata ctccttttgt caatcatttc
    50041 ctttttctct tttggacatt cacaggttca aacgattcta tggccagaat tgaatggcac
    50101 agcactatgg gattcagtaa ttgctaattt taaaccgatt aaaacactgg gctatgataa
    50161 atcccgcgat acaatgtatg ccgtgattga tttaaaaaat ggtggttcac tctcctgtgt
    50221 ctataccggt tacacgatca aacttgatcg taccaaagac ccaagcgccg acgcttataa
    50281 caaattaatt gattgcgaac atacctggcc acaatcaatg ggcgccgatt ccgaacctca
    50341 aaaaagcaat ttacaccatt tgtatccgac ctttaccaac gtgaattctt cacgcggcaa
    50401 tgatcctttt gccgaaatcc cggatgctaa aactgataaa tggtggtatt taaataagtc
    50461 acaaaccata attccaacct ccaatattga taaatactcg gaaaaagaaa acgatccccc
    50521 agcctgtttt gaaccgcgtg aagatcataa aggcaattgt gcgcgttcga tgttttattt
    50581 ctatgccatg tattcggagg catactttac taaagatccc gatatgactt tttggaattt
    50641 tcaaaaagaa actttattag cctggaatta ttacgacacc gttgatgccg acgaatatgc
    50701 gcgtacgtgg aaaatcgcca cttatcagga taacaaaccc aatccattcg tgttggattc
    50761 aaccctcgcc cgcagaatct ggttctataa accgaacaca ttggtaagag agaaaccaag
    50821 tcttgccgat gatttcaaac ttcatccttg ttatccgaat ccttttaata aagccaccaa
    50881 tttggcattc accttaccag aggttgagaa tgttacgcta tccgtattcg atttaaatgg
    50941 tcatttagtt aacgaacaac agttagggcg atttccatcc ggtcatcata ttgtagcttg
    51001 gaatggaggc aacttgagca gtggcgttta catttattgt cttcaggctg ggcaacgcgc
    51061 gcatgctggt aagttattaa ttattaagta atatgttcta tttattaatc cagtaagaca
    51121 tagaaaattt tattcacaaa aactattaca attaacactc taaaaaataa agcttgttta
    51181 actgcagggt ttaaagtatt ttaatacaac gcttttcgaa aaacgagtaa ccatcaatca
    51241 caaaccaacc agaaaaactt tttcacttag tggtagacct tttttgatca cgaaaatacc
    51301 cattttgcgg gtactggcgg aaactacttt ctggagttgg gttatggaac ccgcttagag
    51361 ataagcattt ggtcaatgta aaagaaagta cgttaaagcg ttcaagatca aaaggaggtc
    51421 ataatgaaca tctacgtagg aaatttagca ccgaatgtca ctgaaggtga cttacgcgtt
    51481 gctttcgagg ctttcgggca ggtcgccagt gcatctgtta ttaaagataa attcaccggc
    51541 gaatcaagag gcttcggctt tgtcgaaatg cctttaatgg ctgaagctca agccgctatc
    51601 agcaatttaa acggcaaaga cttgaagggc aaaaccatta ccgtcaatga agctcgtcct
    51661 cgtacagagg gcggtcgcgg tggcgatcgc ttcggcggcc attctggtag cggtcacttc
    51721 ggtggcaaac cgcgggacaa tcggcgcggc ggcggacgct attagtctgc tgtacgtcgg
    51781 ctaaatccaa gccaaattaa taataactgg ctatccacta cttaaataga ggatagccag
    51841 ttatatatgg aattgcaatg caaattccac tggtttattg tttgaactac cgattttaat
    51901 tatattcctg attgttgatt aaaatcgctg gataaagcat gaataataat accgaaattg
    51961 ttgatcttaa aaaagaacag atcagctctc ttgaccgacc acgttataaa tattcgttga
    52021 tggcacgcgc cttcttttta cttatggata ttgttactgg atgtcagatt accttatcta
    52081 aagctaaatt aattgaaatc ctggccagta tcccgtatcg cgcctgggaa gtacgccatt
    52141 attctcgtct gacccgcgtt taccgcaatt atgaaaaagt cactcgaagt caacggattg
    52201 tcacatggag ccgatcagcc caggataatg aatactggca tttactggtc attcacgaaa
    52261 aaatgaaaac cgatggtatt aaagatgctt ggtacctttt tccgctcgtt ccgtttgtaa
    52321 tggttgcagt atatcgcctt tttgccaaaa ctttggcttt ctgcaatatc cggcgcgctt
    52381 atctcttcaa tgctgaattc gaggatcatg ccgaacatgt ctatgcccaa ttcgttgccg
    52441 atcacccgga atgggaaact cagccggtcg aaagcgacct ggtcaatgaa tacggaaaat
    52501 ttagctcctg ggcagatgtc ttccgacgaa tcggactcga cgaacgtgat caccgcaatc
    52561 acagttttct actctgcggt aaaccggaag cagttgtcag ttataatgga atgcccaaag
    52621 atataaattt cccgaccggt cggtagtttt acttgaccgg acttagcctt aatacaataa
    52681 catcgtgagc acctatttga gtcgaaaacg gtttggcagt tgtcccaatc tcctttttca
    52741 accaaaggtc acgaattttg tattcatttt cattaaaaag ataatagtaa tgaaattcgt
    52801 catctttgac cggatatttt ttccaatcga ggtcaacttt caccggttct aacgttcgat
    52861 ttaaaaagca gagtgccaga tcgcctttgt ccagcggctt tgcccagact tcccaattat
    52921 ctttcacaag gtatttaaaa ccctggattc ccaatggatc ctgatcaatg gcaatcaact
    52981 ctttattagt caaaatatct atcacctccg aactcatatc tctgaggtcg ttgcccgcaa
    53041 ttaaaggtgc cgcctgaata caccacaggg aaaaatgtgc ccgatcttcg gcgattgtca
    53101 tcccattgcc gacttccagc atatccggat cgttccaatg atccggtccg gcgtactgac
    53161 ggatattttc gcgcatatcg agaatttgta acacacccca ggaattccag gtaccgtgcc
    53221 cctcctgtcc atcaaaaatg gcagtgatgt caccggtcgt acgccagaga tgcccaatat
    53281 ctttgcccca ttcccaggga cgattatcgc cccactcaca aatactgaaa acaattggcc
    53341 gaccagccgc gaacaaagcg tcacgcatag tcagataagc acctttggca ttaagcccct
    53401 cagtattgca ccaatcatat ttcagataat caactcccca tctagcataa gtaagtgcat
    53461 cctgatattc gtacccacgg ctaccaggat gcctcccgca tgttttccaa ccggcatccg
    53521 aataaatccc aaatttcaat cccttggaat gaatataatc cgccagagct ttcataccgg
    53581 atggaaatct tgccggatca ggttgaataa atcccagagc atcgcgttcc ccttgccagc
    53641 aatcatcaat gactacatac tgataaccag cggctttcat accgctggac accatcgcat
    53701 cggcggtttc ccgaataagt tgttcattga cattgcatcc aaacctgttc caactattcc
    53761 aacccatcgg tggcgtcagc gccagtttgt cgaacttttg agccattagt ggaacacaaa
    53821 acaataaaac cattaacccg cagttttttt tcataaaagc atcccccata tattttgttt
    53881 tcagaaagct tacatttttt ataataaaat ccacttcgga ttgtaagtta aattaaagtg
    53941 aatttcaaga gttttttaga tgaaaatcga attgcaaagc aaggctgaaa atttcttata
    54001 tcaaattttt ataaatgact caactgaaaa attatcgggc taatctgcac caattgcaaa
    54061 ggttaaaaat aaaaaagtac taaactcagt aatatcataa gaggcttcta gcccattagt
    54121 tatcggttgt caaagatatt aaagcccgcg ggtccccatc gttaagatat tctgaaagca
    54181 atgtaaggcg taagcatccg tcatcccagc tatgtaatca cgcacgatca attccgaagg
    54241 acagttttca gcctgatagc gttcatgtaa ggatttgaga tatttgccaa attttacatc
    54301 cagtggtgtt ctactgttag catatctctc atagtcattt tgatattttt cgtacaagtc
    54361 aatcaggtaa tttttgaggg tttcgattac aaagccggag taactccgcc aatattttaa
    54421 cttaggatta tcgtagattt tttcccgact aaattcttta aattcgctaa tcagggcaaa
    54481 tttttccggt gaaaaaccaa ttttgccatt ttttgttgac caatcaatga catcgaaaac
    54541 tagattattg ataatttcac tattggattg tccgatacct tttctcaatt tttccggcaa
    54601 atcttccttg ttaataagat tagccgcgac tgcatcttcc agatcacgtc ccaaataggc
    54661 gattgaatcg ctgaggcgta caacacaggc ttcccaggtg gaaggctgaa ctgggcgaaa
    54721 ttgtaaattg tccagatcgt taagtgtcgg actgatttcg ataaattgct cgtcaacttc
    54781 gccacaatgc gaaacgattc catcacgcac cgcataggtc agattcagcc cttgcccata
    54841 attttcgagt ttatcaacta ctcgcaggct gtgtaattca tgcacgaagc cgccaaatac
    54901 attctccagc catttttcac cggcatggcc aaaaggcgcg tgtcctaaat cgtgtcccaa
    54961 tccgatcgcc gttgctaaat caatgtccaa ttttaaagct cgacaaatcg tagctgaaat
    55021 cgtcgccacg tgtaaagagt gttcgatccg ggtacaaata tggtcatttt ccggggaaaa
    55081 aaagacctgc gttttgtgtt ttagtcttcg aaatgccagt gaatggataa tagccgtttg
    55141 atcgcggaaa aatggcccac gaatatcttc cggtcgctta cgctggcgcg ccgttaaaac
    55201 tcgccgtgga tgttcattct tgaatttcaa ttgcgatttt atcctctata gtcattttta
    55261 ttcctgaata tattacgatg aaaattaaaa atacagcgtt tttcacgctc attacctaag
    55321 ccaaattatc gggtaagaat ctctttgatt aaaacgtcag attcttcttc aattccgaaa
    55381 gtcggacgac atggcggcgc tcttcgtcaa tgattccgct aatcaattga tgctcactgg
    55441 ctggaattaa ctccttgatt tctttataat acagaaccga atcgagttct ctttcaatgg
    55501 cataaagcaa tgccgtttta gcatcattaa ttgctggtat tttggtttca tcattggcat
    55561 cagaaaaaat caggttctgg gtataggctt ccagataatc gtaaaattcc tcgcgagccg
    55621 ccgattccag ctttactgca ccgattttct gcgccatttg ccggaatatt ttcttatgca
    55681 cgacctcttc gtcagccagt ttttcgaaaa gttctttgac ttctgctttc gaaataactt
    55741 ttgccgtttc gcggtaaaat ttttcgcctc tttcctcaat tttaaccgca atgtcaaata
    55801 catcacggat gctgaaaata gtcatcaatt atctccttaa gaacttaaag cacggtgtct
    55861 ggcggcaatg tcatccgcca atctatctag ggcagaaaag tcctccaatt tgggataacc
    55921 tttaataatt accggagaca gaaactcggc tttcattcca cttagcaggg ctttgatctg
    55981 ctcgggcatt tttccacccc agccaaaaga gccgataatc gtcaagtacc gggtcttagg
    56041 tcgtaaaagg tttactaaat aagccgcact gagtgccgct ggatgcggac cgagaatcac
    56101 agtcggcgtc ccaatgacca ccgtagccgc atccaccacc gccattgcca attcccctat
    56161 atcggttctg gacaggttaa aaggctgaac cgtaatctca cgctcaatca gagccttcgt
    56221 gagatactga accatcgctt cggtactgcc atgcattgag acatacggta taagcactat
    56281 atttttaacc gaatccgaag tccaatcggc ataagcctcc ataataaatt ccggatttgg
    56341 gtaaatcggt ccatggctag gggcaatcat tgcaatggcg agattcttta ctttttccag
    56401 attttgacgc acattcgagc gaaacggcat cataatttcg gcataatagc gtttagcgga
    56461 gcgataaact cgactttcat catcaacaaa taaattactc gttgctaagt gcgaaccaaa
    56521 aaaatcacag gagaacagga ttttatcttc ttccagatag gtcgaaatgg tttccggcca
    56581 gtgaacccag ggagtaaaaa taaaacgtaa agttttattc cctaagctga gttnnnnnnn
    56641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    56701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcatcaa acaaccggcg atcccaatcg
    56761 cgggcaccaa cgctaaaaat atccggcttt aaaatctgga ctgccaattt cagtcctcct
    56821 gtggttcaaa atcctctttt tcagcaccac actgcgggca aacccagtca tccggtaaat
    56881 cctcaaaagc agtgccccgg ggcacaccac tatccggatc ccctttgcga ggatcataaa
    56941 cataaccaca aacagtacaa acatatttag tcatattacc tcctgtaaat gagttaataa
    57001 attctatttg atcaataatt ttcagccaat agttcaaagt aagctcgagg gtgggcgcag
    57061 gctggacatt tctccggtgc cttagtaccc acatgaatat aaccgcagtt tcgacaacgc
    57121 catgtcacaa cctgctcctt ttcaaaaacg cggttttcca acacattttt acgtagagta
    57181 cgataatttt tctcgtgctg ggtttcagca acaataacgg ctcgaaaaac attggcaatt
    57241 tcggtaaacc cttcctcatc agcgacttta gcaaattcag gatacattac cgaattttca
    57301 tatttttcac cattggcggc ttccagcaaa ttctcggctg tcgtgccaat tactccggct
    57361 ggaaatgcgg cggttatgct aacctcaccg ccttctaaaa atttgaagaa acgcttggca
    57421 tgttccttct cattatcagc cgtttctgca aataccgcgg caatttgttc atagccttct
    57481 tttttggctt gagaagcgaa ataattataa cgattacgag cttgagattc accggcaaaa
    57541 gcggtcaaca agtttttttc cgtgcgggtt ccttttaaac ttggcatata tttctccttt
    57601 cttattatta attatcatgt tcaacggggt aaccaaccga ttcgatagct tcaatcagta
    57661 tcgccaaggg agtgtcagtc tccactacta ccttttttga agttaaatca aaagcgatat
    57721 tagccggatt gacatacgga ctcaaggctt tctcgatccg cattttacaa tgctgacagc
    57781 tcatcgtcgg aattttgaac gtatatttca taaatcactt tttcacagga taaaattaat
    57841 catagaatcg tgattttaaa atcagattat tttttgctaa aaaacctgct caacgctctt
    57901 gatttcggga atagcttctt tcaagcggcg ctcgatgccc atccgcaagg tataagtgct
    57961 cattggacaa ccgccgcaag cacctgttaa tcttaatttt acgactccat ctttggtaac
    58021 ctcgactaat tcgacattac cgccatcagc catcaacatc ggccggatgg attcgattac
    58081 ttctgcaact ttctctttca tttaggttcc ctttctattg atttagacag tttcgacata
    58141 ttcctttgaa atagccatga atttcctgga ttttatgtcc atcaacttcc ttactttcaa
    58201 ataacggaca ttgaacgtca acatcataaa ttcggttgca ctgtaagcag agaaaatgat
    58261 ggtgttgatt ggttgagatt tcatagcgga cttcggtgcc ggtaatcata atcggcgcta
    58321 ttagtccttt ttccagaaat aaattcaagg tattataaat cgtcgtccgc gacatcgttg
    58381 gcatcttttt taacagactt tcatatatcg catcggcagt caaatgctca ctactttgtt
    58441 ccattaattc taaaacacat aaacgctgga aagtcggttt aatatcgcgc ttttccaatt
    58501 cacctttaat atttatcaac tttgaactca ttacaagtta atattaacaa cagttatgga
    58561 aaaattcaag taatattttc catttttaca tcaaatctct tttaagatta gttaattttg
    58621 tttattttct gatgtaaatt ttttggaggt ttttgtatga gtaaaattga agaatatctt
    58681 ggaaaagatg cggattttct actgaatcat aagtgcatga caatttccaa ggaactattg
    58741 catttacctg ggcctgattt tgttgaccgg attttgcttt attcggatcg gccagtaggc
    58801 gtcctgcgca atttgcaaac aatgtataac accggtcgtt tgtcaggtac tggttatctc
    58861 tcgattctgc cggttgacca gggcatcgag cattccgccg gagcttcatt tgccccgaat
    58921 ccgatgtatt tcgatccgga aaatattgtc aaattagccc tcgaaggcgg atgtaatgcc
    58981 gtagcttcga cgctcggcgt tctggggagc gtcgcccgta aatacgccca caaaattccc
    59041 tttctgctga aacttaatca caacgaactg ctatcctatc ctaatatgta tgatcagacg
    59101 atgtttgcca gtattgatca agcttttgat atgggcgcag ttgccgtggg tgctacgatt
    59161 tattttggtt cggcggaatc acgtcgtcag attatggaag tcagtgaagc attcgaatat
    59221 gcgcatcagc ttggtatggt tacggtactn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    59281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    59341 nnnnnnnnng gttgctatat gggacgagct ggattgatta attccggtgg cgccagcggt
    59401 gaagacgatt taattgaagc cgtaaaaaca gccgttatca ataaacgggc tggcggtatg
    59461 ggtttgattc tgggacgtaa agcttttcag cgaccaatgg ctgaaggcgt caagatcgta
    59521 aatgccgtcc aggatgttta tctcgacaaa aatgttacga ttgcctaacc tgaatcaatg
    59581 gggcaatcta taccgaggct atccttggat aataaaatca agatggtaaa gccccgttcg
    59641 gggctttacc tttttcgagg taggaaatga ctaatcaatc ttcgcatccc aattggataa
    59701 tcggactatt tttaattctg ctgggtttgc tgatattctt aattcagttc gattactttg
    59761 actggtctga tttttggccg ctgatcatta tcggagtagg attattattt ttagtgggtt
    59821 tttttgtcaa tcgcaataat tatgggctat tgatgcccgc tatgattctt ttaattgtcg
    59881 ggactttgtt tctttatttg gaaagaacca cttggactaa tatgaattat ctctggccga
    59941 tttttatatt agcaccggga gttggttttt tctttatgta ttttttcgga ccacaaggca
    60001 ataatctctg gattcccgga actatattaa ttgtgatttc cgttattttt ctggctggtg
    60061 aatggactat tttacgctac tggccagtta tattaattct acttggtatt tatctaattt
    60121 ttagtaaaaa accttcttca tgaacccacc ttctcccgaa aattcccaaa tgccaaaaga
    60181 cacacccaat cctaagtcca attggtggca aaagtggaaa acttctcaac gcaataaaat
    60241 aattgccgga ataattgcct taattccggt tgtgttgaca atttatttca ttaagctagt
    60301 tatttctttt ttcgatcgga tcgttagccc ggtacttgat cctctgattg gtttccactt
    60361 gcccgggttg ggtttaatcg tggctcttat agtcatctat ttgcttggtc tttttattac
    60421 taatttcatt ggaaaatcat taattctcct aattgagaaa tggcttaatt acattcccat
    60481 cgttcgcaca atttataata cgagcaaaca agtaatgaat gctctttcct tttccaaagc
    60541 cggttttgaa aagaccgtta tcgtcgaata tccccgtaaa aatacctggg caattggatt
    60601 cttaacaggc gaagttagcg accataatgg caaacgattt ctgagtattt ttctaccgac
    60661 gactcccaat ccaacttccg gctggattct gctctttccc gaagatgaag tttactcttc
    60721 cgatatgaat gttgaacaag ggcttaaggc tataatatca gctggagcta tcttgccacc
    60781 taaattaaat tttagatcaa ccgataaagt aaacttttcc gagaaaaatg aatcccaaat
    60841 caaacaatag aaacttttaa ttaacttgaa tataaatttt gagaacaaca ttaatagttg
    60901 taaaatgata aaaaatagag aagagaccgc catttatgaa aattaaatac attgatggta
    60961 agagatttat acgtggcatc aaagtcggtg cgtataaaat cgttaaccat caggattatc
    61021 tcaacaaaat caatgtcttt cctgttcctg acgcagatac aggcaccaat atggccgcca
    61081 ctatgaaaac catagtggat aatatccaag aagacacaaa ccagattgat gaagcgacga
    61141 aaaacatcgc caattcagca ctcgaaggag cgcgcggcaa tagcggcgtt attctggctc
    61201 agtttttcta tggattggca aagggaatcg gaaaaaataa acgtcttaca accattcaat
    61261 ttggcgaggc tgtcaattct gccaaaagtc atgcttacga agcgctatcc aaaccgcgag
    61321 aaggtaccat tctatctgtc attaaagatt gggctgaaaa tatttacgaa aacagcaaga
    61381 aaattttcga ttttcaggag ttactaaatc attccctaga agttgcccga cattctttgg
    61441 aaaaaacccg caccaaaatt gaggcgatgc gaaatgccaa cgtcgttgac tccggcgcac
    61501 agggattcgt ttatattctg gaaggaatca gtaattttat caatcgtgga agactcaagg
    61561 agctggaaaa aaattgtatt cagccggtct cagtcatcgc ccgcacggaa gtactaccgg
    61621 aacagattac ctaccgtttt tgtactgaat gtcttatcga aggtaaaaat attccgatag
    61681 aaaacctacg tctggagttc gaaccatacg gcgattcgat tgtcttagcc ggttcaccgc
    61741 agaaggcacg tttgcatatt catactaacg aaccagctaa agtttttaaa attgcccatc
    61801 aatatggcga tctccttcag caaaaagccg acgatatgct taagcagtat ttaattgccc
    61861 ataatccaca tccgaaaatt gcgttggtag tagattcagc ctgcgatttg ccacctgaat
    61921 tcatcgaccg ctcctttatt catattatac cgatacgatt gacgtttggt caatcgagtt
    61981 accttgataa agtcacaatc actcccgaat atttttatga aatgctcgac acggagccgg
    62041 aacatccccg tacttctcag ccatcaccgg ctgattttat caatttatat agtttcttgc
    62101 tcagccacta tgattcgatt atttcaattc acctgccagc cactctcagt ggaacttacc
    62161 agaatgcctt aaaagccgcg caaaattttc ccaaacggaa aattagcgta attgatagta
    62221 aggctctatc ggtttctttt ggttttcttg ccgaactggc ggtggaatta attaccgccg
    62281 gtaaatctca taacgaaatc gtcaatgaat tggaaaaagc tacccatcgg acacaattat
    62341 ttatcggagt accaagtttg aaatatctaa tgcgtagcgg gcgagtctcg aaggctaaag
    62401 gcttgatagc caagttattc aatatcaaac cgatcttaaa tttggacgaa tacggttcac
    62461 ctcaacacat atcgaaaagt ttttccaaaa agggagcttt cactaaagtt ctgcagatgg
    62521 ctagcaattt tgcagctcaa aaagtcaatc cacggtttgc tatcgctcac gccaacaatc
    62581 tgaagattgc tgaatacttc aataatgaat taaaaaaacg tttcaaggtt gaaaaaatca
    62641 atatcttacc tgttgcgccg atgttaggcg cgcatgccgg aaatggcgcg gcggcgatcg
    62701 ctgttacctg gagtgaatga tgataacatt attggcaatt atcgtattca gttatttaat
    62761 cggctcgctc cctacggcca tcatcgtcgg gaaaatcact aaaaaaattg atattcgtca
    62821 atacggttcg tgcaacgccg gcgccacgaa tgcgtttcgc gttctaggct ggagagcagg
    62881 gcttttcgtg gctctttttg acattgccaa aggggcattt actaccgcct acatttccca
    62941 aatccgcttc gatattccgc cgttaaatcc cacctggtta atgattttag ccggtttatg
    63001 tgcaattctt ggccatactt tcaccatctt tgccggtttt aaaggtggca agggtgttgc
//
LOCUS       DAOSMP010000011        57152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_604_length_57152_cov_14.972503, whole genome shotgun sequence.
ACCESSION   DAOSMP010000011 DAOSMP010000000
VERSION     DAOSMP010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 57152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57152
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_604_length_57152_cov_14.972503"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1532)
                     /locus_tag="PKX09_03245"
     CDS             complement(<1..>1532)
                     /locus_tag="PKX09_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16872.1"
                     /translation="VTGTDDSPQEYDDDYSFFDVERDLTYTGDYPNNNSRNPKWQGPV
                     GLVGYAFLESPGNPYDGIDNDGDNKPEEHPGKNLIFTAPLFTESDFDSVVYNKGDQIV
                     LIDKDYKRTFHTITSDTETVVTKGMQITIIAGQTKLVEGNVLANGSINPNVYDGIDND
                     LDGLIDENYLLHYRQRRVDQNGVVLFDTLNPVAYKDYRRGLGIDDPLIDEARNDGLDN
                     DGDWNPETDDVGNDGKPGTNDYGENDGKPTANEPNFDKTDVDESDQIGLTSFNYFVPS
                     NAYPMADDELLWEWLKPGYFDVPSSIQNGKPVAGEDGDFIYGSGYFPLRAGDTQRFSL
                     ALVYGYDLTDLLKNRETVQDIYNSDYRFPQPPDKPTVTAVAGDGKVTLYWDRKAEESI
                     DPVTKEKDFEGYKIYKATDPDFNDVFVITDANGVLTGYKPLAQFDKVNGIKGIFEASS
                     DLYQSAGGYSCYLGSDNGLVHSYVDEDVQNGRIYYYAVVAYDHGDASNDIFPSENTKF
                     ISV"
     assembly_gap    1533..1632
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1633..2488)
                     /locus_tag="PKX09_03250"
     CDS             complement(<1633..2488)
                     /locus_tag="PKX09_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16873.1"
                     /translation="MKHSIKYLTIEILSILLMTITTSQIILANQSGKAYRRSSIHNGN
                     LVRTVFGNWGVIGQPREKGPRGAWLFDTNGYIGDVSPMVGAEVNYHSEELDSTITFHS
                     VVVCPVDRPATAREESPTGDQWTFEPVSGYFNESSQGIAMSTDRNSWPPYWPDKMDDP
                     EDPGWPEQWNGFFGKGVTNADQESYFIMDDNNDVEFNSGHNLKGVYFKPDNRDANRNG
                     LGLEVKVRGMQWAQFLAQDVIFWLYEITNTSTTDYDKVVFGMIAGTYNGVTGTDDSPQ
                     EYDDDYSFF"
     gene            complement(2485..5283)
                     /locus_tag="PKX09_03255"
     CDS             complement(2485..5283)
                     /locus_tag="PKX09_03255"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU16874.1"
                     /translation="MRYKDWLITVVLILTAGYLFGGNTGKITGIVTDSQTGLPLIGCN
                     IMLEDTYLGAASDTDGRYMILNVPPGEYRLKAQMIGYQPKVITGVIVSADLTTSINIE
                     LSTQVLEGEEVIVTAERKMIVKDMTATASHVKAEELAALPVTEVSDVLEMQAGYVDGS
                     LRGGRRGEVAYLIDGIPVTDSYDRSAVIDVNKNMIQELQVISGAFNAEYGKVMSGIVN
                     ITTKQGSNKHGASLEMYIGDYLSTHDEIFDNIGSINPLNIHNAEISFFGPLIKDKLFY
                     NFNTRYIYFGGWLHGQNRYKPTSILAVSPDGSHFLLDSTTMLGDQKWVNMNWNEKLYF
                     QGQLIYRLSNRSSIYYNLFYDNKDYQDYDRMFKYNPAGILKKNLTAETHLLKFQQQLS
                     SKTFFTLAGSYYYRKYTGRLSADTSYNDSTTYVHPAYLYQYPYQFYFGGTQNDHEYRD
                     SRTYLAKWDLTSQINGQHQIKGGLEFQQHDISLRTFNIRPATGETYIDFASGLNIPST
                     WNPYIHPTILDNSTIYASYYRHKPYEFAGYLQDKMEFSELIINIGVRFDYFEPDGVVL
                     ADPSDPEIYEPIKPENRYHDLNGNGVQDPGEPEVTLEERRQYWYKKATPKYQFSPRIG
                     LSFPISDRGVFHFSYGHFFQIPNFNYLYMNPDFDLGSGTGNQGVIGNADLKPEQTIVG
                     EIGVQQQLSDNFSVDITAYLKDIRDLTGTRAEEIIVYGGAASYSRLVNSDFGVVKGIT
                     IALNQQNPSGLYTSFDYTLQIAKGTASDPEAYRNAIAGGSQPEIQLNPLDWDQRHTIN
                     GTVGYNHKNYGINLIGRWGSGLPYTPQSSEDITSLLTNAGTKPPTLTIDAKSYYRFTL
                     SGRYSVELFIRIQNLFDKLNEVNVYDETGRAGFTTDEARVEALNIKTPINSVHEWFTD
                     PTYYSEPRRVEIGMRISI"
     gene            complement(5329..7176)
                     /locus_tag="PKX09_03260"
     CDS             complement(5329..7176)
                     /locus_tag="PKX09_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOU16875.1"
                     /translation="MKNATENQVPKGKFSLARGLLVIGIVAGICLSDNLSDRTYWTYD
                     AIWYQIFPERFRNGDPTNDPTIETLEGTWPYEPQTQWSIMPWTADWYKLQPWEKSNQR
                     DFYYNAQLRRYGGDIEGIIDKLDYLVDLGINAIYLNPVFESASSHKYGATMYYHIDNN
                     FGPDPAGDIAIWQTENPNDASTWLWTSADKLFLKLIQEVHTRQMKIIIDGVFNHVGIP
                     FWAFQDVKKNGEASPYADWFVIKSFDDPSTIEDEFEYQGWNGIPDLPEIWEDENGPVQ
                     GFRAHIKAVVERWGDPNGDGDPSDGIDGWRLDVAEKVSKNFWRDFRKWVKSINPDAYL
                     TGEIWWEDFMNNKMFDASPWLQGDIFDGVMNYRFGDAMLKAFVDREMRIKPSELDQLL
                     GDIREKYPLPAQYQLQNIIGSHDNERFASMLLNPDRWIDHGGNLVYNKDFKINRPSDE
                     LRQIQKTILVFQFTYIGAPYIYYGDEVGMWGADDPDNRKPMVWDDMQYELETTHPFGI
                     KRPVDSVKVDTDLFNFYKSVINLRKNHECLRRGRYKTILTDDKRGIFGFERKYGTETI
                     YIFANLNDKPIKMSEINFDVTKCEKIFTVNTDTNYLGSKGIVVYKIIEG"
     gene            8058..8825
                     /locus_tag="PKX09_03265"
     CDS             8058..8825
                     /locus_tag="PKX09_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU16876.1"
                     /translation="MRKILVVDDDPNIIELITSYFSARGFEVYSSIEGAQVLDLAISK
                     EPDLIILDINLPDIDGLSILKTLKTHPITAFIPVIMLTAKASTEFQVDGLVSGADDYV
                     TKPFDLNVLYARVLNSLRRSLLPTRFKHDQFNLLSYLLRVYSKRGYKVYSKLLDNYSP
                     HPPYWKGYIPDMIIEKPEKLRCFNFETTQSIMEESFLDRLNSLVECHKNTTSHFEATV
                     IVRTMDNYKIVQKLVNEHCLPIKIKYFKKHIRRNLSV"
     gene            complement(8822..9193)
                     /locus_tag="PKX09_03270"
     CDS             complement(8822..9193)
                     /locus_tag="PKX09_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU16877.1"
                     /translation="MPIKPKILIVDDEAVIREIYGDFLEQNGFEVTTALNVETALESI
                     KAKKPDLILLDIVMPFRNGFDLYDQVIVDYPDIKFVFMTGYDDDIKITDRLSKSGKKW
                     FAKPIKLEEMLEVIRNELSAS"
     gene            complement(9194..10567)
                     /locus_tag="PKX09_03275"
     CDS             complement(9194..10567)
                     /locus_tag="PKX09_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU16878.1"
                     /translation="MEDNKPVQNPLREIVDPFVNLIKAPRALWGINLSYLLEGITYFG
                     ILSLLAMYFNDLGLNDIQAGQMVGIQTAGITLAMLFLGATVDWIGVRKALLISLSTML
                     VGRVLLTLAPQTGAPGLWQKAHIVAMLGILGVVLGYGIYQPACYAAVKQFTDKKTSAM
                     GYAMLYAIMNLGGFLPGLISPPIRKNHGIVGVYWVYVALTVAGILVVAFILSKKTVEM
                     AIANAREGQDEPVSEPETNETQSTKEKIVYYLKNFPVKDGRFMFFIFILIPVQTLFAH
                     NWLTLPMYFERAFSGVVSNNFEFFVNFNPILIFILAPMVAALTAQKDTYKMMILGTTV
                     MALPTFLLAIGPNIYSVFGYLIIMTIGEAIWQPRFLQWVAEIAPKNMTGIYMGIGQFP
                     WFLTKVITSLYSGWFLTKYCPAGVTPEQTNTETMWLIYGFIAIISPIALLIARKWMGK
                     GLEKQKA"
     gene            complement(10786..11790)
                     /locus_tag="PKX09_03280"
     CDS             complement(10786..11790)
                     /locus_tag="PKX09_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOU16879.1"
                     /translation="MLNFKIPIPYFLFCVFLLTSCQPKSKLVKAQSTRFAMGTVIEIT
                     VLDTTEAEANNALEAGFAEINRISTQFWEGNPEGPIYAFNHRSTETVTMPLEILELIA
                     RCEAYSTKLDGAFDITVGTISPLYNFKGDSPRPPDLAMIKALLPAVNYQNLTIDFAKA
                     QLGAKNHQTKIGMGAVAKGYGIDQAVAVIAKHNVAGALVNAGGDLRALRRVDGQKWRI
                     GIQDPRQADNILLVIEFDSGAVATSGDYEKFFMYEGKRIHHIINPRTGLPADSCQSTT
                     VIAATAEQADAMATGLFVLGALSGQKALENFPNCEVLWICADGQKVQSAGFEKYLAQH
                     "
     gene            complement(11807..12280)
                     /locus_tag="PKX09_03285"
     CDS             complement(11807..12280)
                     /locus_tag="PKX09_03285"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOU16880.1"
                     /translation="MNNNDQYFKTDDETATVIDADQHWVTVELAEKDTCHSCGAKMIC
                     RPNSTGKRILKIPNTINAKVGDTVLIEQIGANQLKLTIVQYGLPLFGFLFGVLIANRF
                     IKHSIFGIPADIVQMFCGIIVVILTGFVIYFWSKRKAKSGFNVFRLRQTLNGKFE"
     gene            complement(12270..13088)
                     /locus_tag="PKX09_03290"
     CDS             complement(12270..13088)
                     /locus_tag="PKX09_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOU16881.1"
                     /translation="MNSTTFLYSLLSMGGIGLFFSILIAVANKKLHVEEDPRISLIAE
                     CLPGANCGSCGYAGCNNFAEALAGGKSQISNCPVCSPSAREEIARILGVSVEIGERQV
                     AVVFCQGGDDVATKKAEYRGIKTCSAANLVSGGERACSYGCIGLGDCVAVCPFDAIHM
                     DENNLPVVDRAKCTGCGNCVTACPRGIIELHPISHHVFVLCRNRDIGRIARTVCKRAC
                     TGCLLCEKAVNGQGFKVVDNLAVVDHDVYTKELVLPTEKCPTKAIVILGTKDEQ"
     gene            complement(13107..13679)
                     /gene="rsxA"
                     /locus_tag="PKX09_03295"
     CDS             complement(13107..13679)
                     /gene="rsxA"
                     /locus_tag="PKX09_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOU16882.1"
                     /translation="MDLILIFISAAVINNYVLVSFLGICPFIGVSKKLGSAVSMGMAT
                     TFVMTITAAVTWLLNKYILVGLHLEFLQIVSFILVIASLVQLVEMFIKKVSRPLYDAL
                     GIFLPLITTNCAILGLALFLVMKQYNFIESIVFGFASGAGFTLALVIMAGIREELELA
                     DVPEPFKGAPITLIVAGCLALAFMGFSGLI"
     gene            complement(13682..14290)
                     /gene="rsxE"
                     /locus_tag="PKX09_03300"
     CDS             complement(13682..14290)
                     /gene="rsxE"
                     /locus_tag="PKX09_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HOU16883.1"
                     /translation="MAKSKISVTQEFLKGLWNENPILRMVLGTCPTLAVTTSAINGVT
                     MGLAVIFTLVMSSMMISLLRKLIPNEVRIATFIIIIATFVTVADRVLAAFYPEMSKAL
                     GPFVPLIVVNCIVFARQEAFASKNPVGRSVIDALGMSVGFTLVLLIMGSFRELIGNGT
                     IFSHQIMPLTFQPWMAIALQPGAFLTLGFIIGFTNWLNTKRK"
     gene            complement(14292..14882)
                     /locus_tag="PKX09_03305"
     CDS             complement(14292..14882)
                     /locus_tag="PKX09_03305"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOU16884.1"
                     /translation="MNKSLKLVVVLTLTALLSGLILAVLNTYTAPRIELNENKTLLEA
                     IEYVIPGQKTCDVKVIKDVTFYVGKDDKGQVIGVAFVAEGDGFQSRLRILVGMDPEMT
                     KIVQMKILSQAETPGLGTKIENDPTNKTDAFWFLKQFVDLPVVNPISVVKGQAADKSK
                     SQIQAITGATISSRAVVDIVNAAIEKNRALYLEKVK"
     gene            complement(14879..15910)
                     /locus_tag="PKX09_03310"
     CDS             complement(14879..15910)
                     /locus_tag="PKX09_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOU16885.1"
                     /translation="MTEQQNIVKDGAGKEPKVKPTPNPVDKSVEKRLFILSSSPHIKA
                     STDVSSIMWLVVATLVPTVIFSIYNFGWRCLTVIATSMITAMIAEELFNLIRRRKMTI
                     YDGSAALTGLLLALTLPPGLPLSMVIIGAAFAIVVGKQIFGGLGHNVFNPALLGRAFL
                     QTSFPIAMTTWTIPRTIDAITAATPLGSYKFEKTLTPYLDLFIGKVGGCIGETSALAI
                     LIGGLILIILHISNWRIPVSYLGTVVVFSGILWLINPAQYPDPIFHLFSGGLMLGAFF
                     MATDMVTSPITNLGAWIFGIGAGFLVVLIRQFGGLPEGVMYSILLMNGFVPLINRYTR
                     PKYFGEVKS"
     gene            complement(15954..17303)
                     /gene="rsxC"
                     /locus_tag="PKX09_03315"
     CDS             complement(15954..17303)
                     /gene="rsxC"
                     /locus_tag="PKX09_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOU16886.1"
                     /translation="MKLKTFKKGVYPDEFKSLSDYKSIEKSPLPTEVFIPLQQHVGAA
                     CVPVVEKGMEVKTGQLIGKSGGFISSNIHASISGKVKTIERLPHPLGNNSLMINIIGD
                     GHDEWVELPDSISDWENLTPEQINKIILDAGIVGLGGAAFPTQVKLNPPKNKTIDTFI
                     LNGCECEPYLTADHRIMVEHSEEVVLGLRIMMKALGVSRAIIGIEANKPDAIAALTKA
                     TEKFWGIIVQPLKVKYPQGAEKMLIDAVLNRKVPAGGLPMDVGVVVNNVGTALAVAEA
                     VTKRKPLIERVVTVTGQGVREPKNILARIGTPFQNLIDFCGGLTDDTVKVVMGGPMMG
                     VAQASLAVPVIKATSGIVCLTDKIAVERKEYPCIKCGACLHVCPMNLIPTRLARLSQF
                     AKWDEAEQYGIANCIECGSCAFICPSAIPLVHHIRTGKTRVAEIQRQKRGEKKAVSV"
     gene            17463..17816
                     /gene="ssrA"
                     /locus_tag="PKX09_03320"
     tmRNA           17463..17816
                     /gene="ssrA"
                     /locus_tag="PKX09_03320"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(17795..19069)
                     /locus_tag="PKX09_03325"
     CDS             complement(17795..19069)
                     /locus_tag="PKX09_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU16887.1"
                     /translation="MKPNKQLLIAHYTLPPAIGGVESILKPFAEVFARNGYLVTILSG
                     AGSIEGQNIKTSLLPDLNPNSPHVREIQRVLKFGSLPEFYEYQMQNLQRRIEAEIGNI
                     DTIIIHNIMTMPFNLMATEAFWNYILKYPNKKYYIWTHDLAWLIDEYKPNLYDRRPWS
                     LLRTEIPNVTYVTVSEYRKRQIAELLNIPRRKIHVVPNTIKYQDFFKFEPATNIIVEA
                     LKIFSFYPIILVPARIIPRKNLERSIKVIAALRNTWPNLVGIITGMPKRENGALTEYA
                     ASLYNLVQENNLSNNLFFLGDLFERLNVPAEKNRAVVRDLYFISNLVLYLSMDEGFGL
                     PILEAGIARTPLALSQIPVFREVAKEGTLYLPLDESPEYNANRIVKYLTDNQPRSDVL
                     YKSIIQKNNWDDLWESHLNSIFNESMSGGGGN"
     gene            complement(19114..20049)
                     /locus_tag="PKX09_03330"
     CDS             complement(19114..20049)
                     /locus_tag="PKX09_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16888.1"
                     /translation="MFIKYYPNKYERHQLIEDLKIIRDKGYDGVFYCINKPYETGDLT
                     IKYFPVARMIELTKLAGLKAGIILDCFQNPFLWQMDIFSPPVNYLGTAYAPQKGYYPV
                     CPNNPLGHDFYHLLLQKTYRLGQIDYCMFENLRFPFFWQEENLDIQHRVPPYCYCPFC
                     VTEFSSIVGEVVSFTSQIVEMLPDWLEWRSGIIANLVFDACDALIKRTKIIISTPPLA
                     LIDLPFATGQLPLAYVDNGCLISPALYHPSKMGNELWIEDVLDQYRIEFKMNKLFPTF
                     KVQSESDLKQYLAIDSTENFAGLLFDNWQSLKYEN"
     gene            complement(20103..20178)
                     /locus_tag="PKX09_03335"
     tRNA            complement(20103..20178)
                     /locus_tag="PKX09_03335"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20140..20142),aa:Pro,seq:ggg)
     gene            complement(20238..20624)
                     /locus_tag="PKX09_03340"
     CDS             complement(20238..20624)
                     /locus_tag="PKX09_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOU16889.1"
                     /translation="MEKPIKKLYYSIGEVSEITGLKQYVLRYWEKEFPSLTPTKNRAG
                     NRAYREKDIQLIQYIKHLLYDKRFTIEGAKQKLKEMKPEDWQKAIDTENRNELDKTVE
                     ERTDTAVVLSEIKKGLNELLEMLNNW"
     gene            complement(20708..21373)
                     /locus_tag="PKX09_03345"
     CDS             complement(20708..21373)
                     /locus_tag="PKX09_03345"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOU16890.1"
                     /translation="MLNRGYEFATLSNLQANKNKPNLIALTFDDGYQDIYLYAFPILK
                     SLGIPATIFIITDYLDRPNTWDINLGGIHFNHLTYDNIAELIQNGWEIGSHSISHHLL
                     VGMPTLEMQAEIHDSKKLLEERFNCEVRFFCAPFGKLNPKIISEAKSAGYQGICGFFP
                     FRYYHQKPPDFFIPRLAVYSFDSLNAVARKLATNWQLRREIIKQNVVNFCANGTIIVQ
                     KLR"
     gene            complement(21506..22759)
                     /locus_tag="PKX09_03350"
     CDS             complement(21506..22759)
                     /locus_tag="PKX09_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU16891.1"
                     /translation="MNRKIIPARRTEKVTYAIRDIAVKAKHLEDQGRKIIYLNIGDPP
                     VYDFETPFELRQIVSEKILQSSANSQLNVSVYGDSMGLIEARDAVARYATEKKGIKGV
                     TANDVFMANGASEAITLAIAALINPGDNIMTPSPGYPLYSGQIPVYHGNLNPYLLNES
                     TGWQIDFDELEKRVNKRTKAIVVINPNNPTGANYNRESLLNVINFACRHNLVIFADEI
                     YDKLLLDGQTHTSIASLADDVPVVTFGGLSKNYIMPGWRVGWAIFSDPTAVMADYREA
                     INKLLRARLCAVHPQQYMVAPALNGNDSHLPQVIQKLTIRRDITDKMLNEIPGISCVK
                     PEAAFYAFPRIQLPPGVTDEQFVLELLNDTGVLVVYGSGFGQQRGTNHFRVVFLPDEN
                     ILRHSYTLIGEFTAKFYRKYNFKPE"
     gene            complement(22799..24040)
                     /locus_tag="PKX09_03355"
     CDS             complement(22799..24040)
                     /locus_tag="PKX09_03355"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOU16892.1"
                     /translation="MISENIQRTILPNGIRVLSEYVDSVYSVSLGFWIQAGSQDEEDN
                     ENGIAHLLEHMVFKGTHKRSAFAIAHEIESLGGIINAFTSRNVTCFNVQLLHENLAKG
                     VEVLSDLILHGRLDPHELEKEKRVICEEIREIEDTPHDLIHDLFIQQLFPNQAMGRPI
                     QGTVESVNAIQVSDLKRFLKRHYTTGRLVVSAAGRVNHEQLVKLVEKYCQDMGNGADY
                     QEKDSGITQPETQKIYPKPIMQSHVILGRRIFGQGDSRRFQLGLLNMILSGGMTSRLF
                     HNIREKYGYVYEVYSFSDLFLNEGLFGIYAGVDKNRLDMVIDKINNEMTALYRKPIPV
                     REYKKAKQQVKGGLVLAMENMNARMSNLAKTEIYEKHIITIAELLEIIDKISIEDLQE
                     LARYLFDRNAFIETIIQPIES"
     gene            complement(24044..26239)
                     /gene="pnp"
                     /locus_tag="PKX09_03360"
     CDS             complement(24044..26239)
                     /gene="pnp"
                     /locus_tag="PKX09_03360"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOU16893.1"
                     /translation="MYQKKMTLGGRELSIETGRMAKQASGAVVVRYADTMILVTAVAN
                     FDAVTEQDFFPLQVEYRERAYAAGKIPGGFFKREGRPSEKEILGSRLIDRPIRPLFPE
                     DFHCDTQIIANVISSDQENPADVLGIIGASTALMISEIPFSGPIAGVRVGRLNGEFIL
                     NPTIKQMEEGELDIVVAGTEDSVVMVEGECKIISEDLLIEAIEFGHSAVRELIQFQKD
                     IVAELKPVKRPIEPVEEPLELKNAVSAQTETMLADWRQLAQVSKKSWNDAIRTFVNKL
                     TEEYAEQYPEKGNYIGQVVDEIVKKDMRRLIIETGKRLDGRALDQIRPITCEIGILPR
                     AHGSALFTRGETQSLGSVTLGTKLDEQIIDDIDREEFTKKFMLHYNFPPFSVGEVKPM
                     RGTGRREIGHGNLAERALKTQIPSEEEFPYTIRIVSDILESNGSSSMATVCAGSLAMM
                     DAGIPVKETVAGIAMGLIKEDNKLIVLSDIIGAEDHYGDMDFKITGTRTGITAIQMDL
                     KIQGIAASTMRGALEQARVGRMQIIDIMEQTISKPREKISDFAPRMLTLKVDNDLIGE
                     IIGPGGKNIRALQTESNSKIEIDDNGMVFISAADFASAEKARQLIIDMIAKPEEGKIY
                     HDCPIKRLESYGAFVEFLPGKEGLLHVSEIAWRRTEDINSVLKVGDKVDVLLKKIAAP
                     GRFELSMKELVEKPEGYQETPRNSHQPRQSHSDRSKGDHHSSHRTHPRY"
     gene            complement(26258..26527)
                     /gene="rpsO"
                     /locus_tag="PKX09_03365"
     CDS             complement(26258..26527)
                     /gene="rpsO"
                     /locus_tag="PKX09_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOU16894.1"
                     /translation="MTLTKEIKSELVSKYGKNSHDTGAITAQIAMLTERIKDLSQHFA
                     AHPKDHSSRRGLMKMVSRRKHLLEYLRRKDNAAYKELIEKLELRK"
     gene            complement(26524..27492)
                     /locus_tag="PKX09_03370"
     CDS             complement(26524..27492)
                     /locus_tag="PKX09_03370"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOU16895.1"
                     /translation="MNIYYGIENIPADYNGACVTLGVFDGLHLAHRQIIQKVIELATE
                     QNSKAMLITFEPHPRRVLSQSSANHLPILTTVDEKIELLEKIGIEEVLFIKADRTFLN
                     TEESVFVQEILVKKLKVSAVVVGYDYHYGRDRKGNPQTLTAAGKKFGFRVIVMPPFAM
                     NGILVRSSLIRELITEGKILTANQMLGWRYSFKGKVIRGTGRGHKLGFPTANLVVNPP
                     DKLIPGDGVYFVKACLSNFEYFGILNIGMRKTFNESERVIELFIINFKDEDLYDREIK
                     VIFIERLRDELKFETSEALKKQMQADLEICLAKINKLENKTEVCFK"
     gene            complement(27489..28220)
                     /gene="truB"
                     /locus_tag="PKX09_03375"
     CDS             complement(27489..28220)
                     /gene="truB"
                     /locus_tag="PKX09_03375"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOU16896.1"
                     /translation="MPRISKSYSKKYINRKRIIKTDWSNKILNVYKPAGISSYQVVRR
                     VKNLLKIKKVGHSGTLDPFAEGVLLILIGGATKKMNDLLKLPKSYEAILRLGEMTATG
                     DNTAPIIKTAPVPSITPENLIEITKNFTGIIEQIPPIYSAKKINGRPSYKYARKGQEI
                     KLQPTQVNIYELDLRLLDYNTIFIKVTCSSGTYIRKLGEDLAEALGTVGHLTNLKRTR
                     IGNYDWKEAVPYSELNGYLLENATV"
     gene            complement(28147..28521)
                     /gene="rbfA"
                     /locus_tag="PKX09_03380"
     CDS             complement(28147..28521)
                     /gene="rbfA"
                     /locus_tag="PKX09_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOU16897.1"
                     /translation="MTGYRIKRVEDEIKRIVAEIFIRDIRLDNVGLITVTKVVCSSDL
                     REAKIYMSIYNNQKYSIAEAMKIIKSHTSFVRGLLGNRIMMRFVPRITFYYDETQEYA
                     ENIEKLFQKIHQPEEDNQNGLE"
     gene            complement(28521..28802)
                     /locus_tag="PKX09_03385"
     CDS             complement(28521..28802)
                     /locus_tag="PKX09_03385"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOU16898.1"
                     /translation="MTIGLLQIELRLKNTHSLKDKRSIINRIKNQVRAKFNVSIAEIE
                     PNDQYGHCLLGITTISNDQKIVHQTLNSVEYLVENFAEIQIIGREMEMF"
     gene            complement(28819..31347)
                     /gene="infB"
                     /locus_tag="PKX09_03390"
     CDS             complement(28819..31347)
                     /gene="infB"
                     /locus_tag="PKX09_03390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOU16899.1"
                     /translation="MSIDPKDKPKRIFEIAKELNISHNDIIKYLEKEGIPCKSIMTPV
                     DESSYMKILGEFAKEKDIVERLRLERARREAENRRKAEEDARRTAEMERRKLEEEVYK
                     SALLFFNSAIKDVFKYSLNVQSQLIESVWETAAVTDRLPEIPKEPEITEIAEPSIEKT
                     TTTTPPLESPKKKGKKKLRRIKIDELETRLDLKHGKHKPTETGDEVSHRRKKPGNERE
                     ANDRVVDAQIRKTMAKMDEKSTKKRHKKTETVAEESEVSKKIRISDFATVDSLASLIG
                     IDAADVIQKCIELGTFVTVNQRLDFDTISVLCDEFGFEVEKMEHYGEDQIKIEDTKED
                     LENASPRPPVVVIMGHVDHGKTSLLDYIRRSNVVAGESGGITQHIGAYKVELPNKKFI
                     TFLDTPGHEAFTAMRARGAQVTDIVVLVVAADDGVMPQTIEAINHAKAANVPIVVAIN
                     KIDKPEADVERVKRDLANYGVLVENWGGKVQTAEISAKTGKGIDHLLELILLEAEMLE
                     LKANYQTLAKGIVIESSIDKRRGPMATVIIKKGTLKIGDPFVCGAAFGKVRTILDERG
                     QKLTKAEPSDPVVISGFDFPPKPADIFAVVQDEREARKIATERQKIEHEHQKRQVKEW
                     SLDTISAQIAEGKIKQLNVILKGDFDGTVEAIGQTLQDLGNEEVAVNIIHKAAGNITE
                     SDVLLAKTSQAIVIGFNVSANPKVNDLAKKENVEIRHYSIIYELTDDIKAALEGLLTP
                     EKIEEHLGEAEVREIFKIPRVGEVAGSYVIEGKVTRNATARLKRDGEVIFEGAVESLK
                     RFKDDVREVAEGFECGISLQGAPEFKVGDRIEFFEVKSVKRTLA"
     gene            complement(31352..32743)
                     /gene="nusA"
                     /locus_tag="PKX09_03395"
     CDS             complement(31352..32743)
                     /gene="nusA"
                     /locus_tag="PKX09_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOU16900.1"
                     /translation="MVNREIIEAFSQIAREKNVDRNELGDIIEKVFLTLIEKKYGSTE
                     NFDVIVNMDKGEIEIYHQMTIVDNPTGSPHEITLENARKTEPDLQVGDPFIEVIDPMS
                     FGRRLISHAKQILNQQIVRVEKNQIYEEYAKKIGQIVIGDVHQIRRDAIFILIGKAEL
                     KMPREEQIPNERYRRGENIKAIIKEAIMGPSGPEIIVSRADKSFLVKLFENEVPEIYD
                     KTIEIKKVARHAGDRSKVIVESNDRRIDAVGACVGIKGSRIQAIVRELNNEKIDIINY
                     SSEPEILITRALSPAKPLELEIDEEKKTALAIFADDEISVAIGKMGQNIRLASEVTGY
                     TIDAIKKSDYELTESETLYLEDIEGITEHNWKLLYSYGIETAEEFLDTPREELLSIKG
                     LGEKTLDKLEPIIIEAQEKRKAEVATIHEAAKAQREAALQEAQENKEKQTVEEETDLS
                     DEDIIDDFGQEEK"
     gene            complement(32766..33224)
                     /locus_tag="PKX09_03400"
     CDS             complement(32766..33224)
                     /locus_tag="PKX09_03400"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16901.1"
                     /translation="MELETTKNAARAIVDKIVERFDAYVVGFKVNRQGYRTFVKVLIT
                     TDHGIALDQIAEISRAVRDDVAFNELFPDNFQLEVSSPGIDFPLRNFRDFHRNLGREM
                     NVFHHSENLKSPLKGKLESVDEDQLVIANAEGTHTLKMGEIDFAKVIIQW"
     gene            complement(33310..34005)
                     /locus_tag="PKX09_03405"
     CDS             complement(33310..34005)
                     /locus_tag="PKX09_03405"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU16902.1"
                     /translation="MLKPKKKITRKELKKDPVLEKISQIENLVRKQRKLVSYILLGVV
                     VVVLLTILLVNSKKKANTAAMGELGLAEMSFARNDYDDAILRLENLIEKYSRTRSSGM
                     ATILLARCYLAKDDYENARVNFEKYIDDYKDDKFLTAGAYSGLAICAEQDSNFALAAE
                     YFEKGGKISPHKFHKHELLLNSARNYLKVNNLEKAEEIVNIILNDEPESVNKNSAELL
                     LAQITTRKGLALK"
     gene            complement(34034..34106)
                     /locus_tag="PKX09_03410"
     tRNA            complement(34034..34106)
                     /locus_tag="PKX09_03410"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34070..34072),aa:Arg,seq:cct)
     gene            complement(34246..35400)
                     /locus_tag="PKX09_03415"
     CDS             complement(34246..35400)
                     /locus_tag="PKX09_03415"
                     /inference="COORDINATES: protein motif:HMM:NF027594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HOU16903.1"
                     /translation="MNVTKRFLRDEELAVYLQQLDIKLEKVDDNYRAEALILHQNDPE
                     KGYPKLIKNMQIYSQVAITELIVKTIPVHVMLNKTRRPSLVSSSFGENTDTIKKTCEL
                     CKLDRGQRGIIIKVGDEQEFIILTNPGITYPGDLTIAALKHDKQLITGRFNDMIQISR
                     ALYHFSIYFNGILAGASSPHFHFQAGYKDKLIGEIQIQKLLAGQSVGQARLKRILKSG
                     ELEVFYIENFLRPVHIVVTKNAEALLKFFDHYLILLKDVSQNIKGIKNVPDYVSLIPA
                     LGMRENEPRLNIMLKYFPEYGGFILALFPKRYNRPSCYFRKGNSQILIGMGIKEALGN
                     IITGRETDYKRLRNNPALISQIYTETSISPESVEILNQNLKKINILPRNR"
     gene            35951..36124
                     /locus_tag="PKX09_03420"
     CDS             35951..36124
                     /locus_tag="PKX09_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOU16904.1"
                     /translation="MSREISPISKNIKKLRKQKDLAQDRLSKLADISYNTVIKLESGG
                     ITYPSIGTLQKLA"
     gene            36229..36981
                     /gene="arsM"
                     /locus_tag="PKX09_03425"
     CDS             36229..36981
                     /gene="arsM"
                     /locus_tag="PKX09_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOU16905.1"
                     /translation="MKDDEIKQIVKTKYSNIAKGSCGCGNVSSCCSNSLSTKQISESI
                     GYSEEEINSASEANLGLGCGNPVALSTIKEGDVVLDLGSGAGFDAFLAAKKVGNKGKV
                     IGLDMSEEMTHKAKENAKMYGYQNVEFRLGDIEHIPIEANTIDVIISNCVINLAPNKE
                     KVFSEAYRVLKSGGKMFVSDIVLLEELTQEQRNDKELIAGCVGGAILKDEYIKLIQNA
                     GFKVKVLAEDKKISKRQYNGIKLESLKIEAAK"
     gene            37248..37388
                     /locus_tag="PKX09_03430"
     CDS             37248..37388
                     /locus_tag="PKX09_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16906.1"
                     /translation="MTKEQKFYKALQDVYQKIYLERTPKDYQWAWFTLAARQQPSKKL
                     KS"
     gene            complement(37391..37480)
                     /locus_tag="PKX09_03435"
     tRNA            complement(37391..37480)
                     /locus_tag="PKX09_03435"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37444..37446),aa:Ser,seq:tga)
     gene            complement(37597..38070)
                     /locus_tag="PKX09_03440"
     CDS             complement(37597..38070)
                     /locus_tag="PKX09_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOU16907.1"
                     /translation="MGIEIERKFLVIANDYRTFSPGVRYRQGYLSTVKERVVRVRTVG
                     QQAFLTIKGINVGAVRPEFEYEIPRQDAEQILENICEKPILDKIRYRVKHSGFIWEVD
                     EFLGDNAGLVIAEIELADENQSFIKPDWVGAEVTGDPRYFNSNLIKNPYKSWNIK"
     gene            complement(38072..38692)
                     /locus_tag="PKX09_03445"
     CDS             complement(38072..38692)
                     /locus_tag="PKX09_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16908.1"
                     /translation="MKDSIQKNIANQINNRVIPRWEWRTFGSEFPKAEKFLSSLPYDT
                     IKESEEIYILSKNSNENCKIRNSLMDIKIIEQTNPDGLELWKPVLKASFPLSFEIVRL
                     ILRTLHVRKPLAMRDHYTHHQFLNEIVASNPKLSLVDVKKKRRCYTLEGAEVELSELQ
                     VGNQSVKTIAVESEKPQNVQKVLKLLELDNLPNTNYIKALKKMKRH"
     gene            complement(39048..39545)
                     /locus_tag="PKX09_03450"
     CDS             complement(39048..39545)
                     /locus_tag="PKX09_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HOU16909.1"
                     /translation="MKNKWMSVILILLLASSLFSAGKEWYSFNEGIALAKKENKHIVL
                     DFYTDWCGWCKVMDQKTFSVPTVSKYLFDNFVPIRINAENDKEKLVFQGKTYTPRQLT
                     RAFQVTGFPSIAFITPEMEVIEVIPGYIEKDLFLNLLKFVHQECYKTGISFDEFVKNG
                     CQGSK"
     gene            complement(39678..40883)
                     /gene="hydF"
                     /locus_tag="PKX09_03455"
     CDS             complement(39678..40883)
                     /gene="hydF"
                     /locus_tag="PKX09_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOU16910.1"
                     /translation="MQSTPKSNRLQIGIFGRRNVGKSSLMNALTSQYVSIVSDVAGTT
                     TDPVEKPMELPPLGPVLFIDTAGVDDEGSLGGQRIARTRKILERVDLGIIVAEGKNWN
                     KYEDDLLKELQNRHIPTVVAFNKSDLSQPSENLQAYLKQQNIPYINTIATDGFGVLDL
                     HKILIDTTPADYFNSIGIIGDLVKPGEMAVLVVPIDQEAPKGRLILPQVQTIRDLLDN
                     NAGCIVIKENELTSVLNNLKQPPAIVVTDSQAFLRVAAETPADIPLTSFSILFARYKG
                     DLAEYIAGTRTIDKLKPGDEILIAEACTHHPITDDIGRVKIPRWLNQYVGGQLNFTHY
                     QGHDFPENLSKYKLVIQCGSCMLNRREVLSRIWYCKQARVPVTNYGLTIAYTLGIFER
                     ATALFNIET"
     gene            complement(40940..41242)
                     /locus_tag="PKX09_03460"
     CDS             complement(40940..41242)
                     /locus_tag="PKX09_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16911.1"
                     /translation="MSISKEILQSAANQTNLPDKLNAIAGKLEEEYGLQVNFCKIYGK
                     RWSYVAGDRNLEFAPQRRQLTSRYGIFYKQNDQINPDKMNEIFTALTQILTEIEND"
     gene            complement(41229..42752)
                     /gene="hydG"
                     /locus_tag="PKX09_03465"
     CDS             complement(41229..42752)
                     /gene="hydG"
                     /locus_tag="PKX09_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOU16912.1"
                     /translation="MQNVKVSEWTKKVIKPDEIEKYLIEGKDFIDETVITSQLKSAAN
                     PDKSRVREILAKSLEIERLEPEETATLLNVTDDDLWEEMYLAGLAVKRKVYDNRIVFF
                     APLYCSNLCVNNCAYCGFRYENKEEKRRILTMGKIRKETEAVINEGHKRMIVVFGEHP
                     RSAVDYIAETIRTIYDVKVPVKTGFSAIRRVNVNAAPMSIADLKKLWEVGIGTFQVFQ
                     ETYHKPTYANLHPLNTIKGNYRWRLYALHRAMEAGIDDVAIGALFGLYGWRFEVMGLL
                     YHTIDLERHFGIGPHTISFPRLTPASGSWFSRNSQWRVSDADFKKLVTVLRLSVPYTG
                     LIVTARESAEMRREVIGVGCTQTDASTRIDIGGYTSALQALAKKEKADQQIDEQQFTL
                     GDTRSLDEVVRELAEQGLITSFCTAGYRCGRTGGKIMNLLKGCVEGKFCKANAVLTFR
                     EYLDDYASPETKAVGERLIQKEIEEIKAMPFFQKGRMLETFERLYQQVASGERDVYI"
     gene            complement(42776..43798)
                     /gene="hydE"
                     /locus_tag="PKX09_03470"
     CDS             complement(42776..43798)
                     /gene="hydE"
                     /locus_tag="PKX09_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOU16913.1"
                     /translation="MQKTEIIEFLQGKDDNWLFNEADRIRRAVFGDEIYLRGIIEFTN
                     YCRQNCYYCGLRRSNRAVQRYRLSHEEIIAAVETAANEKIPTIVLQGGEDPYYSAEWI
                     ADLIRKIIDKYDLAITLSLGEQTPEVLQLWRKAGATRYLLRIETWNRENYGSLRPGKR
                     WEDRLNCLKVLRELDYEVGSGIMVGLPGETLATLADAIIGLTELDLDMIGIGPFLAHP
                     QTPLKDKPNGSIQLTLRTLALVRILNPYANMPSTSAMECAEKGARLAALKIGMNVIMP
                     SITPERVKLLYNIYPGKNADSDASEHSIPAVLEIIREAGFHRSLSKGISPNLIRRHES
                     QNILSK"
     gene            complement(44062..45759)
                     /locus_tag="PKX09_03475"
     CDS             complement(44062..45759)
                     /locus_tag="PKX09_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOU16914.1"
                     /translation="MKKVYVPMILCVIFSVIFAQERNPQMTPLDSEFINYIEMVKKGE
                     VKKFTPEGYALGYIPHPLTFETEIPIHLQKQVSLPERYDLREEGKLTSVKDQKDCGCC
                     WTFATYGSIESRWKALELGDYDLSEQNLRNGHGFLLGSCEGGNAKMATAYFSRGNGPI
                     AEVDDPYNVKDSIYVSGLSSQAYVPDARFLPDDINILKQIVYTYGAVYTDMFWDNTYY
                     NSQNHTYYSYVDTSTNHAVLLVGWDDSKETAGGTGAWIIRNSWGSGFGENGFFYISYN
                     DTKVNTSPAFWPNRMDYNPNANVHYYDQLGCVTSFGWVDNSLDQPDYEDYGLVKFVIS
                     TDQKITKVGTWVHSSNSTVNFYIYDNFDGRSLSTLLGSTGDKICDYAGYYTFDLPDSI
                     EVNSGDDIYIKVKYNTPETQYSLPAETFITDYADPVIETGKCWISSTGNNNWLAIGDN
                     TDYKYDLCVKAYGVSTNTGIVGGDDIIPEQILLYQNQPNPFNPVTTIRYSLSTSQYVV
                     LKVYNVLGNEVATLVNEYKTPGNYAVNFDATDLTSGIYLYQMKAGKNIQTRKLMVLK"
     gene            complement(45776..46060)
                     /locus_tag="PKX09_03480"
     CDS             complement(45776..46060)
                     /locus_tag="PKX09_03480"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HOU16915.1"
                     /translation="MKKGDSLEIVLQANPTTGYRWEVASNDTSVLKNIGIEYQADKVP
                     SGIVGSGGKTIMRFMAIKEGETFLQLVYRRPFEKDMPPVKKFELIVVVKH"
     gene            complement(46192..47829)
                     /locus_tag="PKX09_03485"
     CDS             complement(46192..47829)
                     /locus_tag="PKX09_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOU16916.1"
                     /translation="MKYKRSSIILIVVLIAETLLAAEKFPAKDYLKIVTIFTNDMHGG
                     IDRSEASFINPDFPPMLGGGAVAGRYILQKRQEAAQNGWGFLLVDAGDFYQGTPLGTV
                     SEGEAVIEYMNTVGYDALTIGNHDFDNGWKNLKKLAEKANFPFLATNLYRKSTGELAE
                     FVKPYIIKEIQGIKIGIIGATLSATPSMSYPEHIEDLDFRPEIEGIKRYLPELKAQGA
                     NTVIVLTHAWLAYDPEEGYKQMMADLSDGELGESYNTSAQAIAHRIPGIDIMFSGHLH
                     RGFYEPWEDPLNHTLIFQNYANGSNLGHVNFYIHRQTGTLAGYDYESDDGAIFTLFED
                     DFLPDTSLTRLIDFWVQKAEAGFDEVIGLATGQIYRSSSGESPMGNLMADAMLASTKA
                     DIAFSNFGGVRADLPAGQITPRHVFKVMPFGNRIVIMKVTGKFLKELVEDRVSGSNQG
                     MLVGGAEIVIDRNQPDGSRVIKFNIGGKPIEYEREYTLAVTDYLAEGNSGYDLLTTIT
                     PDRINYTGILLRQAMIDYIREHSPIVPKTDGRWREIR"
     gene            complement(47833..49179)
                     /locus_tag="PKX09_03490"
     CDS             complement(47833..49179)
                     /locus_tag="PKX09_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16917.1"
                     /translation="MKKKIFILTFVLIVSSTGLSQSFFSPLIGDLPSVADARSLAVGL
                     LGWGDGGSASALLTNSALLGYSKQKLSLTAGLNAFSVKEKRSFPVQDSYGDFLADNTY
                     VLNQNWFPDFQVGINTKPLARIRLAMLLNHTNLSDFQYEEEVRGSLYGEYNRDPLVGY
                     NRLSNRYSVFNTSLGMAVNPWKSLWLGAALNFITPDFSEQKYEIQVIKPSDYQSSDHT
                     ISYVVEPKFESAITGNFGMAFDVTKHLSLAISYRLPYNTVQKGGLLYLVNDTTQTLPV
                     LAIDSVFTIEKVTYHQPGELRFGLCLKPVNIIPTQLFFEIVYQNWKDASAKYQFRSDA
                     NPNDIPNNFSDVPIPYRNVTKIHLGIEHIFFSGMPLRFGFYHDPNPINAEMNRNWFTA
                     GTAYQFGNFNLEISGAFTTCDYKYHDLFPIAVEKRVEFDTVRESYLIGMATLRYNF"
     gene            complement(49180..51618)
                     /locus_tag="PKX09_03495"
     CDS             complement(49180..51618)
                     /locus_tag="PKX09_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOU16918.1"
                     /translation="MALNIIVGAVELESKIDLNTADEVELAQLPLTRQQLEDILYWRE
                     YIGPFSSIFDLKNIKSIDHETFLKLKELTQISPVFLEESEKRIEDNYYKVEQWISDDG
                     ASENTVNNWIDMLSNPVNINKLSYFELRNLAGLAPSDAVAIVNRQKQGEIKNRTDLRS
                     TEGLTYYGFSNLEDFIRYTDDDNVHRFGGSFSSVVKNITLSQTPSDDADSYAAFVQLD
                     QPLDTYYKMRLHWGTKFRLEGSYLRNLGEETIYYQGTKIPQFKAFFEINNVEIGAVKL
                     NKIVIGDYLAGFGQGVTFEATDFFMPRKTGYGFRNRLNGLSGNSSRITQYGLRGIGAE
                     AQIGNLIGTGFFSINNRDAVVNDDGSFSTLITMYPRMSHGLYGNIPHNMINAVKEMVI
                     GGNAKYSFVPGTYLGLTIYQSLYDRELDLQVTESLLDPSGYSKYLTQIGNSADAEINA
                     SYESSAPSSIWSSAKSIRRVYGLEFSTVISNLTLQGEYAELDKDLKLGDRSGDPSALV
                     VSGYLQFDNFNFLMLYRDYDLDFDNPYQRSYSNYQRFKGTIYEDVYYLKDPSLAYLYS
                     ATAQPQAERGLYLSTRYQLHRSLIATAEQDIWTRVADNAQYYRTVLNLEYRPVFKYRF
                     KVRQKWQHRDKTNTLTPVFYQSNETRLEATMRMSRYNQVKLLYSISFTEFTTRSRLVI
                     NAETGETSYVGNAGSPSDALGLTVTHNVNERLKLIGSVMTYSGFLWNFEDTDFRVFNT
                     DTRALHGWITVFSRLSRDLSLRLKYSFDLHNQLDNVVGGMIDIGADSSNPVVSEVYYD
                     KFYSDFRIQLDYRF"
     gene            complement(51665..52582)
                     /locus_tag="PKX09_03500"
     CDS             complement(51665..52582)
                     /locus_tag="PKX09_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16919.1"
                     /translation="MRTFKHLLIAFLFIIIPIALWAQVFTQVNEIAAPISATGSSNLA
                     NIGSAWAQLNNPAGLCRLTALELVGGLYRPYGLSYYNSAVAIIAMPIGKVGTMAIGYQ
                     GSSTSYSGNSLTSETAFSLSHGFYLQKDLFSTLSLGYTLNLYQLDYGHSAGVSGDGSD
                     GMDLGSGFGFGLDLGIQGSLHERSWIGLFVKNINSPEMGSALSAQRLPRSVAIGLGYE
                     PYYGLTTNFVIYQKIGTDKTQYRAGLEYRIVPWLVLRTGVNTEPNRIGFGFGIEKWGI
                     LADYAYISHPLLPETHQFSLGYSFRKNEK"
     gene            complement(52582..54576)
                     /locus_tag="PKX09_03505"
     CDS             complement(52582..54576)
                     /locus_tag="PKX09_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOU16920.1"
                     /translation="MKKRIILPGLILLPVLLLTAQDHVVISEIVLQPSNAEYIKLYNP
                     TDNAVDLSNYYLTDGTDPSNQKYYYNLPTGTNFWSGSGSDFIVRFPPGYSIEAQSEII
                     IAATTTAAYENYYGSSPGLSVKDDLLNAIDGQNTKGIGTAYLDNAAETLVLFYWDGSS
                     PTVKDVDYILWGSLICAVDKSGIAGYQNDTPVEQQTFTPTHQDGQKLQRISDEGSEPS
                     SGGNGITGHNETGEDLDDTWQVVAVGNTKPKLSSINYSPSSPLVGQVITFSVNVTDDG
                     SIAAVRLIYTFQSETGTVEMSLTTGNTYSVTIGPFSVAGILYYQIEAEDESGLISTSN
                     QNMITIQAPPEMLTIKTIRENWDEYNGQTVTLRGVVTIGSNILITSRTSAYFQDVSGR
                     GLNLFASAITNLMQGDSIEVTGELTEYNGVKELTNWTNSYTVLAQNVPITSTAKVYIS
                     ELANNLTDWEGSYVEIHGTVAERSNPTATNQGCNVVIEDETARTTVRIWNTTGVLYNS
                     LGEIVNEYTDSLLTLGNQVIVRGVVGIYNNAPQILLGYASDVEPYIVGEVGEGRTKIS
                     VAPYPFVPKLGEVIKYTYEYPANCRAIVRVYDLSGRYITTLVDNYFAVSWKREDTWNG
                     RNELNQLVPPGNYILHLQTTNRSTGKSQISIAPVVVGVKF"
     gene            complement(54580..57114)
                     /locus_tag="PKX09_03510"
     CDS             complement(54580..57114)
                     /locus_tag="PKX09_03510"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOU16921.1"
                     /translation="MHQTIKIFLIALILPTLILAGDLFFSEYIEGSSNNKAVEIYNST
                     GTIVDLTNYRIVRSNNGATVVQDSIVLSGTLNDKSVYVVGNPSAASAILSVSNTTHTI
                     TYFNGDDYLGLQKLISGTWTTIDVLGQFGEDPGTAWSVAGVTDAMAEHTLVRKPTVSQ
                     GNTDWTSSAGTSADDSEWEVHPQNTFTYLGSHVWSGGGNVSPMARAGLDRVERFNVTV
                     TLDGSISNDPDGSIVSYTWTQISGTSVTLSATDQAIVTFTSPSSVETLMFELVVTDNE
                     SAIGKDSVTIYVDPSPIIISEYVEGTSNNKYIEIFNASNELINLTNNGYELRTSFNGA
                     NNFSSSFSDWGSRASIPAGSVIVLANAQAAIYPSPDILVPSSLSSPLNFNGNDAVALF
                     RNGLIVDIIGEPTKIDTILDNRTMRRNSTITFGSYTYIESEWIEFGTDNVENLGSHNT
                     NPNAPLVSDITVSPDFITLEDEITVSATITPVVGSITSATIYYGAPGSLINTAVMWKE
                     TGDTCWMGNIPPQTGNSIIEFYIYCKDDQSPANSGKSATQTLIIANSTPETIANVHTN
                     IESLNGKIVTIRGIVTIGAGVLANDYTSAYIQDASGRGLNLYKSGKLYSDITRGDELM
                     VVGYVEKFFTTIEVTNFIYKKISSGNELPPAQVVTVAGANSSDWEGTLIEFSGMVTKQ
                     ETSDEATNISVSAGTDTTIARIPDATGIDVASITLGNSYKFRGVGNKYKSTYQTLIGY
                     AEDIVPLTAIKESKTNALTFKLNPAYPNPFNPATTISWQLDKNGAYEVVVFNVLGQKI
                     DVIASGYATAGSYAKIWNAAKFPSGVYFVQLQAGNRVRTQKMLYLK"
BASE COUNT        16436 a        12441 c        10904 g        17271 t
ORIGIN      
        1 accgaaataa acttggtatt ttccgatgga aaaatatcat tactggcgtc accatgatca
       61 taagcgacta ccgcataata gtagattcgc ccgttctgaa catcttcatc aacgtaggaa
      121 tgcaccaaac cattatcaga gcccaaatag caagaataac cgccagcact ttgatacaaa
      181 tccgaactcg cctcgaaaat tcctttgatg ccattgacct tatcaaattg tgcgagtggc
      241 ttgtaaccag tcagtacacc attagcatcg gtaataacaa atacatcatt gaaatcagga
      301 tcagtagctt tatagatttt ataaccttcg aaatctttct ccttagttac tggatcgatg
      361 ctttcttcag cctttctatc ccaataaagg gttaccttgc cgtcaccagc aaccgccgtt
      421 accgtcggct tatccggcgg ttggggaaaa cgataatcac tattgtaaat atcctgaacc
      481 gtttcccgat tcttgagtag atcggttaag tcatagccat atactaaagc caatgaaaaa
      541 cgctgggtat caccggctcg tagtggaaaa taccccgaac cataaatgaa atcgccgtct
      601 tctccggcaa ctggtttgcc attttgaata ctcgacggca catcaaaata tcccggtttt
      661 aaccattccc agagtaattc atcatccgcc attggataag cattagatgg aacgaaataa
      721 ttaaaactag ttaaaccgat ttggtccgat tcatcaacat cggttttatc gaaattcggt
      781 tcatttgctg taggtttgcc gtcattctct ccataatcat ttgtacctgg tttaccatca
      841 ttaccgacat catctgtttc aggattccaa tcaccgtcat tatcaagtcc atcattgcgg
      901 gcttcgtcaa tcaaaggatc atcaattccc aatccccttc gataatcttt ataggctacc
      961 ggatttaagg tgtcaaataa aactactccg ttttgatcca cccggcgttg tctataatgc
     1021 aacaggtaat tctcgtcaat taatccgtcc agatcattat caatgccgtc atagacattt
     1081 ggattaattg aaccatttgc caggacattt ccttctacta attttgtttg tcccgcaatg
     1141 attgtgattt gcatcccttt ggttacaacc gtttctgtat cactggtaat tgtatgaaaa
     1201 gtacgcttat aatctttgtc gattaatact atctgatctc ctttattata tacaactgag
     1261 tcgaaatctg attcggtaaa taggggtgcc gtaaaaatta aatttttacc aggatgttct
     1321 tcaggtttat tatcaccgtc attatcaata ccgtcataag gattaccagg actttccaga
     1381 aaagcatatc cgaccaatcc aaccggtccc tgccatttag gattacgact attattatta
     1441 gggtaatcac cagtataagt caaatcccgt tcaacatcaa agaaagaata atcgtcgtca
     1501 tattcctgag gtgaatcatc tgtaccagtc acnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nncaaagaaa gaataatcgt cgtcatattc ctgaggtgaa tcatctgtac
     1681 cagtcactcc gttgtaagtt ccggctatca ttccgaagac cactttatca taatccgtag
     1741 tgcttgtatt ggtaatttca taaagccaaa atattacatc ctgtgccaga aactgagccc
     1801 attgcattcc ccgaaccttc acttccaagc cgagtccgtt gcgattcgca tctctattat
     1861 caggtttgaa atagacaccc tttagattgt gaccactgtt gaattcaaca tcattattat
     1921 catccataat aaaataactt tcttgatcag cattggttac cccttttcca aaaaatccat
     1981 tccattgctc tggccaaccc ggatcctcag gatcatccat tttatcaggc caataaggcg
     2041 gccacgaatt tcgatcagta ctcatagcta ttccttgaga agactcatta aaataaccgg
     2101 aaactggctc aaaagtccat tgatcaccag taggcgattc ttcgcgagca gttgccggac
     2161 gatctactgg acagactaca acgctatgga atgttattgt cgagtcaagt tcttcagaat
     2221 gataatttac ttcagcgcct accattggac tgacatcacc gatatatcca ttggtatcaa
     2281 aaagccatgc cccacgcgga cctttttccc tcggttggcc gatgactccc cagttaccaa
     2341 aaactgtccg aaccagatta ccgttatgga tggaactacg ccggtaagct ttcccacttt
     2401 gattagctaa aattatttgt gacgttgtaa tcgtcattaa caaaattgag agaatctcaa
     2461 tagtaagata ttttatcgaa tgtttcatat acttatcctc attcctattt cgactcgacg
     2521 tggttcggaa taatacgttg ggtcggtaaa ccactcgtga accgaattaa tgggagtttt
     2581 aatatttaaa gcttctactc gggcttcatc ggtcgtaaaa ccagctcgac cggtctcgtc
     2641 ataaacatta acttcattca atttatcaaa taaattctga attctgataa ataactctac
     2701 cgaatatctt cctgataagg tgaaacgata gtaagattta gcatcaattg ttagggtagg
     2761 tggtttagta ccagcattag ttaaaagtga tgtaatatct tcgctcgatt gcggagtata
     2821 gggcaaacca cttccccagc gaccgattaa atttattcca taatttttgt gattatatcc
     2881 cactgttccg ttgattgtat ggcgttgatc ccagtctaag ggattaagtt gaatttccgg
     2941 ttgacttcca ccggcaatag cattacgata tgcttccgga tctgatgcag tgcctttagc
     3001 aatttggagc gtataatcaa aactggtata taaaccgctc ggattctgct gattaagtgc
     3061 tatagtaata ccttttacta caccaaaatc actattaact aatcggctat aagaggccgc
     3121 tccgccataa actataatct cttctgctct cgttccggtc aagtctctta tgtctttcag
     3181 ataggcagta atgtcaactg aaaaattatc acttaattgt tgctgaactc cgatttctcc
     3241 cacgatagtt tgttctggct tcaaatccgc atttccaatt actccttgat taccggttcc
     3301 agaaccaaga tcaaaatcag gattcatata taaatagtta aaattgggga tttggaaaaa
     3361 atgaccataa gaaaaatgaa ataccccgcg atcactaatc ggaaatgaca atccgattct
     3421 gggactaaat tgatacttgg gagtggcttt tttgtaccaa tattgtctcc gttcctccaa
     3481 ggtgacttca ggttcgcctg gatcctggac gccgtttcca ttaagatcat gataacgatt
     3541 ttcaggtttg attggctcgt agatttcagg atcgctagga tccgcaagca caacaccatc
     3601 cggttcaaaa taatcaaatc ttaccccgat attaatgatc aactcgctaa actccatttt
     3661 atcttgtaaa tatccggcaa attcataagg tttatgacgg taatagctag cataaatcgt
     3721 actattatcc aaaatagtcg gatgaatata tggattccag gtagatggta tattcaatcc
     3781 ggatgcgaag tctatatagg tctcaccagt cgctggacga atattaaatg ttcttaatga
     3841 aatatcatgc tgttgaaatt ccaaaccgcc ctttatctga tgttgtccat ttatctggga
     3901 agtcaaatcc cattttgcaa ggtatgttct actatcacga tattcgtgat cattttgagt
     3961 cccaccaaaa taaaattgat aaggatattg ataaaggtaa gccgggtgga cataggtggt
     4021 gctatcgttg taactggtat cggcagacaa tctgccggta tatttacgat aataatatga
     4081 gccagccaga gtgaaaaagg ttttagaact taattgttgc tgaaacttta gtaaatgcgt
     4141 ttcagcggtt aggtttttct tcaatattcc tgccggatta tatttgaaca tacgatcata
     4201 atcctggtaa tctttattat cgtaaaaaag attataataa atactgcttc ggttagaaag
     4261 ccggtagatt aattgtcctt gaaaatacaa cttttcgttc cagttcatat tgacccactt
     4321 ttgatctcct aacatagtag ttgagtccag caagaaatgc gaaccatcgg gagacacagc
     4381 taatatacta gttggtttat aacgattctg accatgtagc cagccaccaa agtaaatata
     4441 gcgcgtattg aaattgtaaa ataacttatc ctttattaat ggtccaaaaa aactgatttc
     4501 agcattatgg atatttagag gatttattga cccaatatta tcaaagattt cgtcatgtgt
     4561 actaagataa tctccaatat acatttctag cgacgcaccg tgcttgttgc taccttgctt
     4621 ggtagtgata ttgacgatgc ctgacattac ttttccgtat tctgcattaa acgctccgga
     4681 aattacttgc aattcctgaa tcatattttt attgacatca atgaccgcac tacgatcata
     4741 agaatcggtt accggaatac catctattaa ataggcaact tcacctcttc tgcctccccg
     4801 taaactccca tctacatatc cggcttgcat ttcaagaacg tcgctgactt cagtcactgg
     4861 taaagcggct agttcttcag ctttaacatg tgaagcggtc gcagtcatat ctttgacaat
     4921 cattttgcgc tctgcagtaa ctatcacttc ttcaccctct aaaacctgag tcgagagttc
     4981 aatattgata ctagttgtca aatcagcaga tacaatcacg ccggttatca cttttggttg
     5041 gtaaccaatc atttgagctt ttaaacgata ttcgccaggc ggtacattaa gaatcatata
     5101 tcgtccatct gtatctgatg ccgcacccaa ataagtatcc tcaagcatta tgttacaacc
     5161 tattaaagga agaccagttt gactatcagt aactatacca gtaattttac cagtattacc
     5221 tccgaataaa taccctgcag ttaaaatcag aacaactgtt attaaccaat ccttatatct
     5281 cattctttac ctcaattgaa tttgatttat tgattttatc aaagacaatt acccttcaat
     5341 aattttataa actactattc ctttcgaccc gagataattt gtatcggtat taactgtaaa
     5401 aatcttttcg cattttgtga catcaaaatt tatctcgctc attttgatag gtttatcatt
     5461 caaatttgca aagatgtaga tggtttcagt accatattta cgctcaaatc cgaaaatccc
     5521 tcttttgtcg tcagtcaata ttgttttata cctgcctctt cttagacatt catgattttt
     5581 tctgagattt attacagatt tatagaaatt gaacaaatct gtatcaactt taacggaatc
     5641 tacaggtctt tttattccaa atggatgagt agtctccagt tcatattgca tatcatccca
     5701 aaccattggt tttcgattgt cgggatcgtc tgctccccac atccccactt catcgccata
     5761 ataaatataa ggtgctccga tataagtaaa ttgaaaaaca agaattgtct tttggatttg
     5821 tcggagctca tccgaaggac ggtttatctt aaaatcttta ttataaacca gattgccacc
     5881 gtgatcgatc cagcggtctg gatttaataa catactggca aaacgctcat tatcgtgact
     5941 accaattata ttttgcagtt gatattgagc gggtagcgga tatttttctc taatatcgcc
     6001 taataattga tcaagctcgg atggttttat cctcatttct ctgtcaacaa aggctttaag
     6061 catagcatcg ccgaaacgat aattcatcac gccgtcaaaa atatcgccct gcaaccatgg
     6121 tgaagcatcg aacatcttat tattcataaa atcttcccac catatttcac cagtcaagta
     6181 agcatcagga tttatgcttt tcacccattt gcgaaaatca cgccagaaat ttttactgac
     6241 cttctcagcc acatccaatc gccagccgtc aatcccgtcc gacggatcgc catcaccatt
     6301 tggatcaccc catctttcaa ccacggcttt tatatgcgcc cgaaatcctt gtacaggacc
     6361 attctcgtct tcccagatct ccggtaaatc cggaattcca ttccagcctt gatattcaaa
     6421 ttcatcttcg attgtactcg gatcatcaaa agactttatc acaaaccaat cggcgtatgg
     6481 actggcttct ccattttttt tgacgtcctg aaaagcccag aaaggtatgc caacatgatt
     6541 gaatactcca tcaatgatta ttttcatttg acgggtatgc acctcctgta ttagtttcag
     6601 aaataattta tcagcggaag tccacagcca ggtcgaggca tcattaggat tttcagtttg
     6661 ccaaattgct atatcaccag ccggatcagg cccaaaatta ttatcaatat gataatacat
     6721 tgtagcaccg tatttatgcg acgaagccga ttcaaaaact ggattcaaat aaattgcatt
     6781 gattcctaaa tctacaagat aatcgagttt atcaattatt ccctcgatat caccgccata
     6841 acgacgcaat tgagcattat agtaaaagtc gcgttggttg gatttttccc aaggctggag
     6901 tttataccaa tcagcagtcc atggcataat gctccattgc gtttgaggtt catatggcca
     6961 ggtgccttct aacgtttcaa tcgttggatc attagtcgga tcaccgttcc taaagcgctc
     7021 tgggaatatt tgataccaga tagcatcgta agtccagtat gttctatctg agaggttgtc
     7081 actcaaacaa atcccagcta ctattccgat gacaagcaat cctcttgcca gtgaaaattt
     7141 gccttttggg acttgatttt ctgtcgcgtt tttcaattgt agttctaatc tgagttacta
     7201 ctaaaagaat atagcgtatt aaaatattta agtcaatagt tttttttact gattttgtct
     7261 gagctaaaga tatggtattc acttttggtg ataataagtg ggaagattcc aatcccccac
     7321 tgtcaaaatt ggattaggtg atactaagac cccagcaaat caaatcttga gtaacctcct
     7381 cctccgtcgg gagttgaacc tgtttaactc tggattcaat cctgagtcta aaaatactaa
     7441 agactacacc aaaccctgcc gcggttgaac aatattatca gtaactccac ctagtctcga
     7501 cgcgggagtg ttgacacgaa tagtctaaag cttgcttaaa ggtgctcaac aatcctgtga
     7561 attacaaaaa actattacgc gttaatctaa tctatttcta aaatgtggat ataaggatgc
     7621 tacaaaggat tgtaaaaatt tatcaatttt tgatcataaa caatgaattt ttggtaaaat
     7681 aaaaaagccg ctcttgtaag cggcttatct atagtgtttc tgtgattttg aggtttccct
     7741 ccccgaacgt aaaagagggg gattttgagg gatattacgg cacatccggg gagggggagg
     7801 ggaaatctat cttcttttgg acattatcat taagtcaaat aacaagactt aatttacaaa
     7861 ttttatgcca atattcaaat agcaaataca gttaattaga caaaatattt tagacctggt
     7921 tacaataaat tttcgggtaa tctataatat ttcgtagata actcctcaga gtttaattcc
     7981 tcttaaaaat atattttctt ttttcatcta acttttttta ttgataataa ttgattgttt
     8041 agtgaaatta tagagtaatg agaaaaattt tagtagttga tgatgatcca aacattatag
     8101 aattgatcac atcctatttt tcagctcgtg gatttgaagt ttattcgagt atagaagggg
     8161 cgcaagtact tgatctagct atcagtaaag aacccgattt aattattctt gatattaatt
     8221 taccggatat agacggcctc tctattttga aaactttaaa gactcatcca atcacggctt
     8281 ttattccggt gattatgtta accgcaaaag catctaccga atttcaagtc gatggactag
     8341 tctctggtgc tgacgattat gttaccaagc cttttgatct caatgttctc tatgctcgag
     8401 ttctcaattc actacgacgt tcgcttcttc ccactcgttt taaacacgat caatttaact
     8461 tgctctcata tttacttaga gtatattcaa aacgtggtta caaagtctat tccaaattac
     8521 tggataatta tagccctcac ccgccctatt ggaaaggtta tattccggac atgataatcg
     8581 aaaaacccga aaaattgcgt tgttttaatt ttgagaccac tcaatcaatt atggaagaat
     8641 cttttctgga tcgcttgaat tctctggtag aatgccataa aaatacaacg tctcatttcg
     8701 aggctacagt tatcgttcgg actatggata actacaaaat cgtgcagaaa ttagtaaacg
     8761 agcactgttt accgataaag ataaaatatt ttaaaaaaca tatccgccgg aatctatcag
     8821 tttaagaagc tgataactca ttccgaatta cttctaacat ttcttctaat ttaattggtt
     8881 tagcaaacca tttcttccca gatttgctta aacggtcagt aatctttata tcatcatcat
     8941 aaccggtcat aaagacaaat ttaatatcag ggtaatcaac tattacttgg tcgtaaagat
     9001 caaagccatt acgaaaaggc atcacaatat ctaatagaat taaatccggc tttttagcct
     9061 ttatagactc caaagccgtt tcgacattta aagctgtcgt cacttcaaat ccattctgtt
     9121 ccaagaaatc cccgtagatt tcacgaatta ccgcttcatc atcaacgatt aatattttgg
     9181 gttttattgg catttaggct ttttgttttt cgagtccttt acccatccat ttccttgcta
     9241 ttaataaagc aatcggacta atgattgcga taaagccata aatcagccac atcgtctcag
     9301 tattagtttg ttccggtgta actccggctg ggcaatattt cgtcaggaac cagccggaat
     9361 aaagcgaagt gatgactttg gttagaaacc aggggaactg tccaatcccc atataaatgc
     9421 cagtcatatt tttaggagcg atttcagcta cccactgcaa aaaacgcggt tgccagatag
     9481 cctctccgat tgtcataatg attagatagc caaacaccga gtagatattt gggccaattg
     9541 ccagtaaaaa agtcggtaga gccattacag tagtgcctaa aatcatcatt ttataagtgt
     9601 ctttttgggc ggttaaggcc gctaccattg gagccagaat aaaaatcagg ataggattaa
     9661 aattgacaaa gaattcaaaa ttgttggaaa ccacacccga aaaagctcgt tcgaaataca
     9721 tcggtagagt cagccaatta tgagcaaata gagtctgaac cggaattaga atgaaaatga
     9781 aaaacataaa tcggccgtct ttaacgggga aattttttaa gtaataaaca atcttttcct
     9841 tggttgattg cgtttcattc gtttctggtt cagaaaccgg ttcgtcctgt ccttcccgag
     9901 cattggcaat cgccatctcg acggtcttct tcgataaaat gaaggcaact actaaaatcc
     9961 cagctaccgt taaagctaca taaacccaat atacaccaac aattccatga tttttcctga
    10021 ttggcggcga aattaatcct ggcaggaaac cacccagatt cataatagca tagagcatcg
    10081 catagcccat agcggaagtt tttttgtcag tgaattgctt gaccgcggca taacaggcgg
    10141 gctgataaat gccataacct aagactactc ccaatattcc caacatagca acaatatgag
    10201 ctttttgcca aagtccggga gcaccggttt gaggtgctaa tgttagaagc acacgcccaa
    10261 ccaacatcgt tgaaagtgaa atcaataaag ccttccgcac tccgatccaa tctaccgtcg
    10321 ctcctaaaaa taacattgcc aatgttatcc cggcagtttg aatcccgacc atttgtccag
    10381 cctgtatatc gttcagtccg agatcattga aatacattgc taaaagactg agaataccaa
    10441 aataggtgat tccctccaac aaatacgata aattaattcc ccataaagcg cgaggcgcct
    10501 taatcaaatt aacaaacgga tcaacgattt cacgtaaagg gttctgaacc ggtttattat
    10561 cttccatcta aacatccttt atttttatcg actctgtcca ataactgaat ctataaaata
    10621 ttgacttatc attccaataa aaatttttaa tggcaaaact tctatcgcta attttgtatg
    10681 tcagtgatta tttattatta atattgcatt aaggaatgta aatgaatatt cagaaatcaa
    10741 ttatgaattg ctcgaatatt gttatttaaa tttttagaac ttagactagt gctgtgctaa
    10801 atatttctca aaaccggcag attgaacctt ctggccatcg gcgcaaatcc aaagcacttc
    10861 gcaattgggg aagttttcca gagctttttg tccggataaa gcgcccaaaa caaataatcc
    10921 agttgccatt gcatcggctt gctcagcagt tgcggcaatt acagttgtgg attgacaaga
    10981 atcagccggt aaaccggtac gtggatttat gatatgatga atgcgtttgc cttcatacat
    11041 aaaaaatttc tcataatctc cggaagtagc cactgcccca ctgtcgaatt caattacaag
    11101 tagaatatta tcggcttgac gcggatcctg aatcccgatt ctccactttt gcccatctac
    11161 acgcctcaac gctcgtaaat ctccgccggc attgactaat gcaccagcga cattatgctt
    11221 tgcaatgact gcgacagctt gatcaatacc gtagcctttg gcaaccgcac ccattccaat
    11281 tttggtttgg tgatttttag cacccaattg cgctttcgca aaatcaatgg taaggttttg
    11341 gtaatttaca gccggtagaa gtgctttaat catagcaaga tccggcggtc gtggagaatc
    11401 acccttgaaa ttataaagcg gcgaaatcgt cccaacggta atgtcaaacg caccatcgag
    11461 ctttgtagaa taagcttcac aacgagcaat caactccaaa atttcaagcg gcatagtgac
    11521 tgtttcagtg gaacgatggt tgaaagcata gattgggccc tcgggattgc cttcccagaa
    11581 ttgggtggaa atgcggttga tttcggcaaa cccggcttca agcgcattat tggcttcggc
    11641 ttcggtcgta tctaatacgg ttatttcgat aaccgttccc atagcgaaac gcgttgattg
    11701 agccttgacc agcttgcttt taggttggca acttgtcaac agaaaaacac aaaataaaaa
    11761 gtaaggaata gggattttga aattaagcat tacttcttct gcggaatcat tcaaacttgc
    11821 catttaatgt ctgacgcaaa cgaaagacat taaatccact tttggcttta cgtttactcc
    11881 aaaaataaat gacgaacccg gtcaagataa ctactataat tccacaaaac atctgcacga
    11941 tgtccgctgg aattccaaaa atactatgct taataaatcg gttagcgatt agtactccaa
    12001 acagaaaacc aaataaaggt aaaccatatt gaactatggt taattttagc tgattagcac
    12061 cgatttgctc gattaaaacc gtatcgccca ctttagcatt aatggtatta ggtattttaa
    12121 gaatgcgttt gcccgtagaa ttggggcgac agatcatttt tgcaccgcag gagtggcagg
    12181 tatctttttc tgccagttcg accgttaccc agtgctggtc agcatcaatc accgtcgccg
    12241 tttcatcatc agttttgaaa tattggtcgt tattgttcat ccttagttcc aaggattaca
    12301 atagctttcg ttgggcattt ttcggtcggc aaaaccagtt ccttggtata aacgtcgtga
    12361 tcaaccacgg caagattatc cacgactttg aagccttggc cgttgacggc tttttcgcac
    12421 aatagacagc cggtacaggc acgcttacaa accgtacggg caattctacc gatgtcacga
    12481 ttcctacaaa gcacaaaaac atgatgactg attggatgca attcgataat gccccgtgga
    12541 caggcagtaa cgcaattgcc acaaccggtg catttggcgc ggtctacgac cggtaagtta
    12601 ttttcatcca tatgaatagc gtcaaatgga caaaccgcca cgcaatcacc aagtccaata
    12661 catccgtaac tgcaggcacg ttcaccgcca ctaaccaaat tagctgcaga acaagttttg
    12721 atgccgcgat actcggcttt tttagtagcg acatcgtcgc cgccctgaca aaaaaccacc
    12781 gccacttgcc gctcgccaat ttcgacactc actcccaaaa tccgggctat ctcctcccgg
    12841 gctgatggac tacagaccgg acaatttgag atttgcgatt taccaccggc taaagcctca
    12901 gcaaaattat tacagccggc atagccgcaa ctgccgcaat tggcacccgg taaacactca
    12961 gcaatcaagc tgattctcgg gtcttcttca acgtgtagtt ttttattagc tactgcaatt
    13021 aatatggaaa agaacaaacc aattccgccc atacttaata aactgtataa aaacgttgtg
    13081 gaattcattt cagatacctt ttgagattag attaatccag aaaatcccat aaatgctagg
    13141 gctaaacaac cggcgacgat taaagtaatc ggtgctcctt taaaaggttc aggcacatcc
    13201 gccagttcca actcttcccg gatgccagcc ataattacca gcgccaaggt aaagcctgcc
    13261 ccggaagcaa aaccaaatac gatactctca ataaaattat attgcttcat caccaagaaa
    13321 agtgccagcc ccagaatggc acagttggtc gtgatcagcg gcaggaaaat accgagcgca
    13381 tcatagagcg gacggctgac ttttttgata aacatttcaa ccaattggac cagcgaggca
    13441 attaccaaga tgaaagatac aatttggagg aattccaggt gtaaaccgac cagaatatac
    13501 ttattcaata accaggtaac cgcggccgtt atcgtcatca cgaaggtcgt cgccattccc
    13561 atactaaccg cggaaccgag ttttttcgag actccgatga agggacaaat tccgaggaat
    13621 gacaccaaga cataattatt gataaccgca gctgagatga aaattaatat cagatccatt
    13681 tttacttcct tttcgtatta agccaattgg taaatccgat tataaaaccc aacgtcaaga
    13741 aagcacccgg ttgcagagct atagccatcc agggttgaaa ggttaacggc ataatttgat
    13801 gcgaaaaaat agtaccatta ccaattagtt cgcgaaatga acccattatc agtaatacca
    13861 gagtaaaccc gacactcatt ccgagcgcat caataacaga acgaccgact ggattttttg
    13921 aggcaaaggc ttcctgacgt gcaaatacga tgcaattcac gacaattaat ggaacaaaag
    13981 gtcccagcgc cttgctcatt tcgggataaa aagcagccag aactcggtct gccaccgtca
    14041 caaaagtcgc aattatgatg ataaacgtcg caatccggac ttcgttggga atcaattttc
    14101 ttagaagaga gatcatcatg ctggacataa ccagcgtaaa tattactgcc aatcccatcg
    14161 taacgccatt aatagctgat gttgtaacgg ctaaagttgg gcaggttcct aaaaccatac
    14221 gcaggatggg attctcgttc cagagtcctt tgagaaattc ctgcgttact gaaatttttg
    14281 acttcgccat attatttcac cttctctaaa taaagggcgc ggtttttttc aatagcggcg
    14341 ttaacaatat ctacgaccgc cctcgaagaa atggttgcgc cggtaatggc ttgaatttgc
    14401 gatttacttt tatccgcggc ttgtcccttg acaacggaaa tcggatttac gactggcaaa
    14461 tcaacaaatt gctttaaaaa ccaaaatgca tccgttttgt tcgttggatc gttttcaatt
    14521 tttgtcccga gtccgggagt ttcggcttgg gataatattt tcatttgtac aattttagtc
    14581 atttccggat ccataccgac cagaatacgt aaacggctct gaaatccatc accctctgcc
    14641 acaaacgcca cgccgattac ctgcccttta tcatccttac caacataaaa agtaacatct
    14701 ttgatgacct ttacgtcaca ggttttttgg cccgggatta catattcgat agcttccaat
    14761 aaggtcttat tttcgttcaa ttcaatacgc ggagcagtgt aggtatttaa cacagccaat
    14821 attaatcccg ataataaagc cgtcagggtt aaaacaacta ccagtttgag tgacttattc
    14881 acgattttac ctccccaaaa tatttcggtc gagtataacg attaatcaac ggaacgaatc
    14941 cattcatcaa taaaattgaa tacatgacac cctccggtaa tcctccaaat tgacgaataa
    15001 ggacaaccaa aaatccggcg ccgattccaa aaatccaggc tcctaaatta gttattggcg
    15061 aagtcaccat atccgtcgcc ataaaaaaag cccccagcat caacccgccg gaaaagagat
    15121 gaaagatcgg atcgggatac tgtgccggat taatgagcca taaaattccg ctgaaaacca
    15181 caactgtacc aagatagctc accggaatgc gccaattgga tatatgcaga attattaata
    15241 tcaatccacc gatcagaata gccaaagcac tcgtttcacc gatacagccg ccgactttgc
    15301 caatgaaaag gtcaagatag ggcgtcaaag ttttttcaaa tttataagaa cccaacggag
    15361 ttgccgccgt aatcgcatca atcgtccggg gaatagtcca ggtcgtcatc gctatcggga
    15421 aactggtttg taaaaatgcc cgccctaaca gcgccggatt gaaaacattg tgccctaagc
    15481 ctccaaaaat ttgtttccca acgacgatgg cgaaagccgc cccgattatt accatactaa
    15541 gcggtaatcc tggtggtaaa gtcaaagcca gcaacaagcc ggtcaaggcg gcactgccat
    15601 cataaattgt cattttacga cgtctaatta gattaaataa ttcctcggca atcatagcgg
    15661 taatcataga agtggctata actgtcagac aacgccatcc gaaattgtaa atactgaaaa
    15721 taaccgttgg cactaaagta gccacaacca gccacataat tgaagaaaca tcagtagagg
    15781 ctttaatatg gggcgaagat gaaagaataa aaagtcgttt ctcaactgac ttgtcaacag
    15841 gatttggagt cggttttact ttcggctctt ttccggctcc atctttaact atattctgct
    15901 gttctgtcat tatgataaat ttccttatta aatattcgat attgaacgat atttcacacg
    15961 cttacggctt ttttttcacc tctcttttga cgctgaattt cggctacccg cgttttccca
    16021 gttcggatat ggtgaactaa agggattgct gacgggcaaa taaaagcgca agaaccacat
    16081 tcgatacaat tagctatacc atattgttcg gcttcatccc atttggcaaa ctgagataag
    16141 cgcgccagcc tggttgggat tagattcatt ggacagacat ggaggcaagc cccacatttg
    16201 atacagggat attctttcct ttcaacagcg attttatccg ttaaacagac aattccactg
    16261 gtagctttta taaccggtac cgctaaagaa gcctgagcaa cgcccatcat tggaccgccc
    16321 atcactacct tgaccgtatc gtcagtcagt ccgccacaga agtcaatcaa attctggaat
    16381 ggagtgccga ttctggctaa tatgtttttt ggttcgcgca ctccctgacc ggtaaccgta
    16441 acgactcttt caatcagcgg tttgcgtttt gtaacggctt cagccacagc cagagccgtg
    16501 ccgacattat taacaacaac gcccacgtcc attggtaatc cgccggcggg tactttgcgg
    16561 tttaaaacgg catctattaa catcttctcg gcaccttggg gatattttac cttcaggggc
    16621 tggacaataa taccccagaa tttttcggta gctttggtta gcgccgcaat agcatccggt
    16681 ttattggctt cgatacctat tattgcccga ctgactccga gcgctttcat cataatgcga
    16741 agtcccagga ctacctcttc cgaatgttcg accataatgc ggtgatcggc tgtcaaataa
    16801 ggttcacatt cacaaccgtt aaggataaaa gtatcaatgg ttttattttt gggcggattt
    16861 aatttcactt gcgttggaaa agccgctccg cctaagccaa cgattccggc gtctaatata
    16921 attttattga tttgttcggg agtaagattt tcccaatcac taatcgaatc gggtaattct
    16981 acccattcgt cgtgaccatc gccgatgatg tttatcatta gcgaattatt tcccaaaggg
    17041 tgcggtaatc tttcaatcgt ttttactttt cctgagattg aggcgtggat attgctcgat
    17101 ataaatccgc cgcttttgcc aatcaattga ccggttttaa cttccatacc tttttcgact
    17161 accggtacac atgcggctcc aacatgttgc tgaagcggaa taaacacttc cgtcggtaaa
    17221 ggcgattttt ctatgctttt ataatcactt aaacttttaa actcatccgg ataaacgccc
    17281 ttcttgaacg tcttcaattt cattttaatt ttgctcctac tctaatcaag ctctaaaagt
    17341 caaaaaaatc gagaaaattt aatcaaatta tttcaattat accttaaata ttttgggtaa
    17401 atcctaacgt ttgatagttt gtttataagg agaaaattgc gtaaattcta aagctttttg
    17461 acgggggcga atggattcga cgggattgaa gatctgccga atagcgtgtc gtggttttca
    17521 ggagggccac gttaataaac ctgagaatcg aaaatgccaa ttcttttggc actgtcgctt
    17581 acgcttaagt aagtgaccgt tctgggccgg tcttgcctgc aggctggttc agagcgtcgt
    17641 caaagcaggc tcgttccgtt tatcggtttg cgtaaacgga attaaaaaca gcaaactggc
    17701 ttttaccaag tttgtccgct ggcacggtaa aagcgagatt cagcaacgga ctacacacgt
    17761 agcgattctg cagttctctt tttcggacgg gggttcaatt ccccccgcct ccactcatac
    17821 tttcgttaaa gatggaattt agatggctct cccagagatc atcccaatta tttttttgaa
    17881 tgatagattt atataataca tcgctccgcg gttgattatc tgtcaaatat tttactatgc
    17941 ggttagcatt atattccgga ctctcatcca gcggcaagta tagagtacct tctttcgcta
    18001 cttctctaaa taccggtatt tgactgagtg ccaatggagt tctggcgata cctgcttcaa
    18061 gaattggtaa accgaaacct tcatccatgc ttaaataaag cactaaatta ctgataaaat
    18121 aaaggtcacg gaccacagct cgattctttt ctgccgggac atttaatctc tcgaaaagat
    18181 cgccgagaaa aaacaggtta ttggacaggt tgttttcttg aaccaaatta taaagactgg
    18241 cggcgtattc tgtcaaagcg ccattctccc tcttaggcat tccggtaatg ataccgacaa
    18301 gattaggcca ggtattccgc agtgccgcaa ttactttaat actgcgttca agattcttcc
    18361 ttggaattat tctggctggg accaaaatga ttggataaaa actaaaaatc ttgagtgctt
    18421 caacgattat gttagttgct ggttcaaatt tgaagaaatc ctggtattta atcgtattag
    18481 gaactacatg aatttttcga cgtggaatat ttaacaactc ggctatttgc cgtttacggt
    18541 attcagaaac agtaacgtaa gtaacattgg gtatttcggt tcgcagtagt gaccacggcc
    18601 gtcggtcgta taaattaggt ttgtactcat caatcaacca tgccagatca tgagtccaga
    18661 tgtaatactt tttatttgga tattttaaga tgtaattcca aaaagcttcg gtagccatca
    18721 gattaaaagg catagtcatt atattgtgaa taataatggt atctatatta ccgatttcgg
    18781 cttcgattcg ccgctggagg ttttgcattt gatattcgta aaattcaggt agtgaaccga
    18841 attttaaaac ccgctgaatt tcacgaacat gcggactatt cgggttcaaa tccggcaata
    18901 gactggtctt gatattctgg ccttcgattg aaccggctcc acttaggatt gttactaaat
    18961 agccattgcg cgcaaatact tcggcgaacg gcttcaggat actctcaacc cctcctatag
    19021 ccggtggtaa ggtataatgg gcaatcagta attgtttatt aggcttcaca agaggtttac
    19081 taaatttgct ccggatttca ttcgtaagaa gaattaattt tcgtatttta atgattgcca
    19141 gttatcaaac agaagaccgg caaaattttc agtcgagtca atcgctaaat attgcttaag
    19201 atcactttcg ctttgaactt taaaagtcgg gaaaagtttg ttcattttga attcaatacg
    19261 atattgatct aaaacatcct ctatccacag ttcattaccc attttactgg ggtgatataa
    19321 tgctggtgaa atcaaacatc cattatccac ataagccaga ggcaactgcc cggtagcaaa
    19381 cggtaaatct ataagcgcca gcggtggcgt actaataatt attttagttc tcttgattaa
    19441 tgcatcacag gcatcaaaaa ctagattagc aattattccc gaacgccatt ctaaccaatc
    19501 cggaagcatc tcgacgattt ggctggtaaa agataccact tcaccaacta ttgatgagaa
    19561 ctccgtaacg cagaacgggc aataacaata gggtggtacg cgatgctgta tatccagatt
    19621 ttcttcctgc cagaaaaagg ggaatcttag attttcaaac atacagtaat caatttgccc
    19681 cagacgataa gtcttttgaa gcaataaatg ataaaaatca tgtccaaggg gattgttggg
    19741 acaaaccgga taatatcctt tctggggggc ataggcagtc ccgagataat taaccggtgg
    19801 agaaaaaata tccatttgcc acaaaaatgg attttggaaa caatccaaaa taattccagc
    19861 ttttaagccc gccagtttag tcagttcgat catccttgcc accggaaaat atttaatcgt
    19921 caaatcaccg gtttcataag gcttgtttat acaataaaac acgccgtcat atcctttatc
    19981 acggataatc tttaagtctt caatcaattg gtgacgttcg tatttattgg gataatattt
    20041 gataaacatt ggcatttcgt tctgaatttc gtgaaaaatt tatttaattc aatcgaaatt
    20101 tgtcggagcg agtggatttg aaccaccgac cccctgaacc ccattcaggt gcgctaatcc
    20161 ggactgcgct acgctccgct tgtcaaaaac gccagtaatt taattgataa gaatttattt
    20221 atctacgaaa aattggatta ccagttatta agcatttcca gtaattcgtt aagccccttt
    20281 ttaatttctg acaataccac agcggtatcg gttctctcct ctactgtttt atccaattcg
    20341 ttcctattct cagtatcaat agctttttgc caatcttctg gtttcatttc tttcagtttc
    20401 tgtttagcgc cttctatagt aaaacgttta tcatacaata ggtgtttgat atattgaatt
    20461 aattggatgt ctttctcacg ataagcgcgg ttgcccgccc ggtttttagt cggtgttaat
    20521 gatggaaatt ctttctccca gtatcggaga acatattgct tgagcccggt aatttcactg
    20581 acttctccaa ttgagtaata taatttttta attggctttt ccatatcaca cctgttatca
    20641 ctattattta aaccattact ttaatagtat aatcagttta aagggttatg tcaagttatt
    20701 tttttattta tcgcaatttc tggacgataa tagtgccgtt ggcgcagaag tttacgacgt
    20761 tttgtttgat aatttcacgg cgcaattgcc aattggtagc cagcttcctt gccacagcat
    20821 ttaatgaatc aaatgaataa actgccagac gtggaatgaa aaaatcagga ggtttttgat
    20881 gatagtatct aaatgggaag aatccgcaaa taccctgata accagcagat ttggcttcag
    20941 aaataatttt tgggttcaat ttcccgaaag gagcacaaaa gaatctgact tcgcaattga
    21001 atcgttcttc aagtaatttc ttggagtcgt gtatttcggc ttgcatttct aaagtcggca
    21061 ttccgactaa tagatgatgt gatatgctat gcgagccgat ttcccaccca ttttggatta
    21121 attctgcgat gttatcatat gtcagatggt tgaaatggat tccacctaga ttaatgtccc
    21181 aggtatttgg ccgatctaaa taatcggtga tgataaaaat agttgccggg atacccagac
    21241 tttttaatat cggaaaggca tataaataaa tatcctgata accatcgtcg aaggttagag
    21301 cgatcagatt cggtttattt ttattagcct gaagattgct aagagtggca aattcataac
    21361 cacgattaag caaaaactcg atatgttttt gaaatttttc tggtttgatg caactcgcgc
    21421 cgatttccga acgccaatca attttatgat agacaaggac tcgattcttt atgggaacaa
    21481 actcgtctat ggctccaccc tttgactact ccggtttgaa attatatttg cggtagaatt
    21541 ttgcggtaaa ttcgccgatt agcgtataag aatgacgcag aatattttca tctggtagaa
    21601 aaactacccg aaaatgatta gtccctcgtt gctgaccgaa accgctaccg taaaccacta
    21661 atactccggt atcattgagc aattcgagga caaattgttc atcagtaacg ccgggcggca
    21721 attgaattcg gggaaaagcg taaaaagccg cctcgggttt aacgcaagaa atgccaggaa
    21781 tttcatttaa cattttgtct gtaatatcac ggcgaatcgt cagtttttga atgacttgag
    21841 gtaaatgaga gtcattacca ttcagggctg gagccaccat atattgttgc ggatgtaccg
    21901 cacaaagtct tgcccgcaag agtttattaa tggcttcgcg gtaatctgcc atcacagcgg
    21961 taggatcaga aaatattgcc caaccaaccc gccaaccagg catgatataa ttcttactca
    22021 atccaccaaa ggtaactacg ggtacatcat cagctaatga ggcaattgag gtgtgcgttt
    22081 gaccgtcaag aagcaattta tcgtaaattt cgtcagcgaa aatcactaag ttatggcgac
    22141 aggcaaaatt gatgacattt aataaacttt cgcgattata attcgcacca gtcggattgt
    22201 taggattaat gacgacaatt gccttagtgc gcttgttgac ccgcttttct aattcgtcaa
    22261 aatctatctg ccagccggtt gattcattta ataaatatgg atttaagtta ccatgataaa
    22321 ccgggatttg gccgctataa agtggatagc caggcgaggg ggtcatgata ttgtcgccgg
    22381 gattaattaa agccgctatc gccagcgtaa tggcttccga agctccatta gccataaaga
    22441 catcattggc tgttacccct tttattcctt ttttttcagt agcatagcga gcaactgcgt
    22501 ctctggcttc gattaaaccc atcgaatcgc catagaccga gacattcaat tgggaattgg
    22561 cactcgattg taaaattttt tcacttacaa tctggcgcag ttcaaaggga gtttcaaagt
    22621 cataaactgg aggatcgcca atatttaagt atataatttt tctgccctgg tcttctaagt
    22681 gcttggcttt cacagctatg tcacggatcg cataagtgac tttttcggta cgcctcgctg
    22741 gaatgatttt ccgattcata taattttctc ttttatgact ttttgccatt tatcataatc
    22801 aggattctat cggctgaata atggtttcaa taaaggcgtt ccggtcgaat aagtagcgtg
    22861 ccaattcctg taaatcctca atgctgattt tatcaataat ttccaataat tcggcaatcg
    22921 taataatatg tttttcgtaa atttctgtct tagctaaatt actcattcga gcgttcatat
    22981 tttccatcgc cagtaccagc ccacccttga cttgctgttt ggctttttta tattcacgaa
    23041 caggaattgg tttacgataa agcgcggtca tttcattatt aattttatca ataaccatat
    23101 ccagtcggtt tttatcaact ccggcatata ttccgaataa gccttcgttt aaaaatagat
    23161 ctgaaaatga gtaaacctcg taaacgtaac cgtatttttc acgaatgtta tgaaataaac
    23221 gcgatgtcat accgccactt aaaatcatat tgagtaaacc gagctggaat cgtcgcgaat
    23281 cgccttgtcc gaaaatccgg cgtcccaaaa taacatggct ttgcattatc ggtttgggat
    23341 aaattttctg ggtttctggt tgagtaatac cggaatcttt ttcttgataa tcggcaccat
    23401 ttcccatatc ttgacaatat ttctcaacca gttttactaa ttgttcatga ttgactcttc
    23461 cggcggctga tacaactaaa cgaccggtcg tataatgacg cttaaggaaa cgttttaaat
    23521 cagacacctg aatggcattg acactttcga ctgttccctg gatcggacgc cccattgctt
    23581 gatttgggaa aagctgctga ataaataaat cgtggattaa gtcatgtgga gtatcctcga
    23641 tttcgcggat ctcttcgcaa ataactcttt tttccttctc gagttcgtgg ggatcaagcc
    23701 gaccatgcaa aataaggtca gataacacct caacaccttt agcaagattt tcatgtaaca
    23761 actgcacatt aaagcaagtc acattccggc tggtaaaggc attgatgatt ccacccagcg
    23821 attcaatttc atgcgcaata gcaaaagctg aacgtttatg cgttccttta aaaaccatat
    23881 gttcaagcaa atgcgcaata ccgttttcat tatcttcttc gtcttgagaa ccagcttgta
    23941 tccagaatcc taacgaaact gaataaaccg aatcgacata ttccgatagg actcggattc
    24001 cgttaggtag aattgtacgc tggatatttt cggaaatcat cgcttaatag cgggggtgtg
    24061 tccgatggga tgaatgatga tcaccttttg aacgatcact gtggctttgc cttggttggt
    24121 gagaattacg tggcgtttct tgataacctt ccggcttttc aaccaattct ttcatcgaaa
    24181 gttcaaaacg tccgggggcg gcgatttttt tcaatagaac atctacctta tcgccgactt
    24241 tcaagacgct attaatatcc tcagttcgac gccaggcaat ttcagaaaca tgcaacagac
    24301 cttctttacc aggtaaaaat tcaacgaagg caccgtaact ttccagcctt tttatcggac
    24361 agtcatgata aatcttgcct tcttccggtt tagcgatcat atcaataatc aactggcgtg
    24421 ctttttcagc cgatgcaaaa tctgcggctg aaatgaaaac cataccgtta tcatcaatct
    24481 caatttttga attactttca gtttgcaaag cccgaatatt cttaccgccg ggaccgatta
    24541 tttcgcctat taaatcgtta tcaactttta aggtcaacat acgcggtgcg aaatcggata
    24601 ttttttctcg cggtttgctg attgtctgtt ccataatatc aataatttgc atgcgaccga
    24661 cacgagcttg ttcgagggcg ccacgcatag tcgaagccgc aattccctga attttcagat
    24721 ccatctgaat tgcggtaatg ccggtacgag taccggttat tttgaaatcc atatcgccgt
    24781 aatggtcttc cgcgccaatg atatccgata atactatgag tttattatct tctttaatta
    24841 atcccatcgc gataccagct accgtttctt taaccggaat tccggcatcc atcatagcta
    24901 gcgaaccggc acaaaccgtt gccattgaag acgagccatt cgattccaga atatcggaaa
    24961 cgatccggat tgtataggga aattcctcct cgcttggaat ttgagttttt agagctctct
    25021 cagccaaatt accgtgtcca atttcccgtc ggcctgtgcc tctcattggt ttgacttcac
    25081 caacgctaaa tggcggaaaa ttgtaatgaa gcataaattt cttagtgaat tcttcgcggt
    25141 caatatcgtc aatgatttgc tcatccaatt tcgttccgag agttaccgaa cccagacttt
    25201 gagtttcacc gcgagtaaaa agggctgagc cgtgggctcg tggcaaaatt cctatttcac
    25261 aagtaatcgg tcgaatttga tccagtgcgc gaccgtcgag tcttttgccg gtctcgatta
    25321 tcagccgccg catatctttc ttaacgattt catcaactac ctggccgatg taattgcctt
    25381 tctccggata ttgttcggca tattcttctg tcaatttatt gacgaaagtt cgaatggcat
    25441 cattccagga ctttttagaa acctgcgcca attgacgcca gtcggctagc attgtttccg
    25501 tttgagcgct aaccgcgttt tttaattcca gcggttcttc aaccggttca atcggccgtt
    25561 tgactggctt taattccgcg actatatctt tctgaaactg aataagttcc cgaacggcgg
    25621 aatgtccgaa ctcaatagct tcaattaaca aatcttcgga aattatttta cattcacctt
    25681 cgaccattac aaccgaatct tcggtaccag ccacaacgat atcgagttca ccttcttcca
    25741 tctgcttgat agtggggttg agaataaatt ccccatttag tcgaccaacc cgtactccgg
    25801 caattggacc ggaaaatgga atttctgaaa tcatcaaagc tgtcgaagct ccaataatac
    25861 cgagtacatc agcgggattt tcctgatcgc ttgagataac gttcgcaatt atctgagtat
    25921 cacagtgaaa atcttccgga aagagaggtc gaatcggtcg gtctatcaac cgtgacccca
    25981 gaatttcctt ttccgatgga cgtccttcgc gtttgaaaaa accaccagga attttaccag
    26041 cggcataggc tcgctcccga tattctacct gtagagggaa gaaatcttgt tcggtaacgg
    26101 catcaaaatt ggctaccgct gtcactaaaa tcatcgtatc ggcataacga acaaccacgg
    26161 ctcccgaagc ctgtttagcc attcggccgg tctcaatact taattcacga cctccaaggg
    26221 tcatcttttt ttggtacaat tatttactcc caattattta ttttcttaat tcaagtttct
    26281 caattaattc tttataagcc gcattgtctt tgcgacgcaa atattccaaa agatgcttcc
    26341 gacggctaac cattttcatc aggccgcgcc gcgaactatg atctttagga tgagccgcaa
    26401 aatgctggct taaatctttg atcctttcgg ttagcatagc aatctgggct gtaatcgctc
    26461 cagtatcatg gctgtttttt ccgtacttgg atactaattc acttttgatt tctttagtca
    26521 atgtcattta aaacatacct ccgttttatt ttctaattta ttaattttcg ctaaacatat
    26581 ttccaaatca gcttgcatct gtttttttag tgcctcactc gtttcaaatt ttaattcatc
    26641 gcggagtctt tcgataaaaa taactttaat ttcgcgatcg tacaaatctt catccttaaa
    26701 atttattata aataattcaa ttacccgctc agattcgttg aaagtctttc gcatcccaat
    26761 attcaaaatt ccaaaatatt cgaaatttga aaggcaagct ttaacaaaat agactccatc
    26821 acctgggatt aatttatccg gtggatttac aacaagatta gcggttggaa aaccgagttt
    26881 gtgaccacgc cccgttcctc taataacctt tcctttaaaa ctatatcgcc agccgagcat
    26941 ttgattggca gtaaggattt tcccttcggt tataagttct cggatcaacg atgaacgtac
    27001 cagaatccca ttcattgcaa acggcggcat aactattacc ctaaaaccga actttttgcc
    27061 cgccgcagtt aaagtctggg gattgccctt tcgatcccga ccataatgat aatcataccc
    27121 cacaacgaca gcgctcacct ttaacttttt aactaaaatt tcttgcacga ataccgattc
    27181 ttcagtattt agaaaagttc gatcggcttt aataaaaagg acttcttcta ttccaatttt
    27241 ttcaagtaat tctatttttt catccactgt tgtcaatatc ggtagatgat tagccgacga
    27301 ttgactaagt acccgacgtg gatgaggttc aaaggtaatt aacatagctt tgctattttg
    27361 ctcggtggca agttcgataa ccttttgaat aatctggcga tgcgccaggt gcaaaccgtc
    27421 aaacacaccc agcgtcacgc aagcgccatt ataatcggca ggaatatttt caatgccgta
    27481 ataaatattc ataccgtagc gttctccagc aaatatccat ttaattccga gtagggcacg
    27541 gcttctttcc agtcataatt gccgatccgg gtgcgcttca ggttggtcaa atgacccacc
    27601 gtgccgagcg cctctgcaag atcttcgcct agtttacgaa tataggttcc actcgaacag
    27661 gtaactttaa taaaaatcgt attataatct aatagcctca aatccaattc ataaatattt
    27721 acttgagttg gttgcagttt aatttcctgt ccttttcggg catatttata agacggacga
    27781 ccattgattt tcttggcgga ataaattggt gggatttgtt cgatgatgcc ggtaaaattt
    27841 ttggtaattt caatcaaatt ttccggtgtt attgacggaa ccggggcggt cttaataatc
    27901 ggagcagtat tgtcaccagt ggcagtcatt tcacccaaac gtaaaatcgc ctcataagac
    27961 ttgggcaatt tcaataaatc attcattttt ttggttgccc cgccaattaa aattaggagc
    28021 actccttccg cgaacggatc gagtgtcccg ctatgaccga ctttttttat tttaagaagg
    28081 tttttaacac gacgtactac ctgataggaa gaaataccag ccggtttata aacgtttaaa
    28141 attttattac tccagtccgt tttgattatc ctcttccggt tgatgtattt tttggaataa
    28201 cttttcgata ttctcggcat attcttgagt ctcgtcataa tagaaagtaa tgcgtggcac
    28261 gaaacgcatc ataattcgat taccgagcag accacgcacg aaggaagtat gactcttgat
    28321 aattttcatt gcttctgcaa tcgagtattt ttggttattg taaatgctca tgtagatttt
    28381 ggcttctctt agatcagagg agcaaacaac tttcgttacg gttattaagc caacattatc
    28441 gagccgaata tcccgaatga aaatctcagc cacgatgcgt ttaatttcat cctcaactcg
    28501 ttttatgcga taaccagtca ttagaacatc tccatttccc tgccaataat ctggatttcg
    28561 gcaaaattct caaccagata ttcgacagag tttaaagttt gatgcactat cttttgatca
    28621 ttactgatgg ttgttattcc gagcagacag tgaccatatt gatcatttgg ttcaatttcc
    28681 gcaatggaga cattgaactt tgctcttact tggtttttaa tgcgattgat aattgaacgt
    28741 ttatccttta aagaatgggt atttttcagg cgcagttcta tttgtaacaa tccaatcgtc
    28801 ataacaattt acgcccattt aagccagcgt ccttttgacc gatttaactt caaaaaattc
    28861 tatgcgatcg ccgaccttaa attcgggtgc tccttgcagt gaaatgccac attcgaaacc
    28921 ttctgcgact tctctgacat cgtctttaaa tcgtttcaac gattcgacag caccttcgaa
    28981 aataacctct ccgtcgcgtt ttaaacgggc tgtggcgttg cgggtaacct tgccctcgat
    29041 tacataagaa ccggctacct ctccaacccg cggtatttta aatatttcgc gcacctcggc
    29101 ttcaccgaga tgttcttcaa ttttttcggg agtcaataag ccttccagtg cggctttgat
    29161 atcatcagtc aattcataaa taatcgaata atggcgaatt tcgacatttt ctttcttagc
    29221 taaatcattg acttttggat tcgcgcttac attaaatcca attacgatag cctgggaagt
    29281 tttggcaagc agtacgtccg attcagtaat attaccggcg gctttatgaa taatgttcac
    29341 ggcaacctct tcattcccaa gatcctgcaa agtttggcct atcgcctcta ctgtaccatc
    29401 gaaatcacct tttagaatca cattgagctg ttttattttt ccctcagcaa tctgagcgga
    29461 aatagtatct aatgaccact cttttacctg gcgtttctgg tgttcatgtt caattttttg
    29521 cctttcggta gcaatttttc gtgcttcacg ttcatcctga accactgcaa atatatcagc
    29581 cggtttaggg ggaaagtcga aaccagaaat cacaaccgga tcggatggtt cggctttggt
    29641 caatttctgg ccacgttcat ctaagattgt tcgtactttt ccaaaggcag ctccacaaac
    29701 gaatgggtca ccgattttta atgtcccctt ttttattata acagttgcca tagggccacg
    29761 gcgtttatca atgctggatt cgattacaat gcctttagcc aatgtctggt aattggcttt
    29821 gagttccagc atctcagctt ctaacagaat taattctaat aagtggtcta ttccctttcc
    29881 cgttttagct gatatttcgg ctgtctggac tttcccgccc cagttttcta ccaaaacgcc
    29941 ataattggca agatcacgtt tgactcgttc gacatctgct tctggtttat caattttatt
    30001 gatggctact acgattggta cattggcggc ttttgcgtga ttgattgcct cgattgtttg
    30061 cggcataacg ccgtcgtcgg cggcaactac cagaactact atatcggtga cctgagcacc
    30121 gcgggcacgc attgcggtaa aggcttcatg ccccggagta tcgagaaagg taataaattt
    30181 cttattaggc aattcaactt tatacgcgcc aatatgctga gtaattccac ctgattcacc
    30241 tgcgactaca ttcgaacggc ggatataatc gaggagagag gtcttcccgt gatcaacatg
    30301 tcccattatt acgacaactg gcggacgggg agaggcattt tcgaggtctt ccttagtatc
    30361 ttcaatcttt atttgatctt cgccatagtg ttccattttt tcaacttcaa aaccgaactc
    30421 gtcacataga accgagattg tatcgaaatc aagacgttgg tttaccgtta caaacgttcc
    30481 cagctcgata catttttgga ttacatcggc ggcatctatc cctattaagg aggcgaggga
    30541 atcaactgta gcaaaatcac ttatgcgaat ttttttgcta acctcagatt cttcagcaac
    30601 agtttcagtt tttttatgtc tttttttcgt tgacttttca tccattttag ccattgtttt
    30661 gcgaatctga gcatctacta cgcgatcgtt agcttcccgc tcattacctg gttttttgcg
    30721 acgatggctt acttcatctc ctgtttccgt cggtttgtgt ttgccatgtt tcagatccag
    30781 acgagtttcg agttcatcaa tttttatccg acgcagtttt ttcttacctt ttttcttagg
    30841 agattctaac ggaggagtgg tagtagtagt tttttcaata ctgggttctg cgatttcagt
    30901 gatttccggt tcttttggta tttcgggaag tctatcggta accgcggctg tttcccagac
    30961 agattcaatt agctgacttt gaacgtttag actatattta aatacatcct taatggcact
    31021 attaaaaaac agcaaggctg atttatagac ttcttcttcg agtttacgcc tctccatctc
    31081 agccgtacga cgcgcgtctt cttccgcttt acgtctattc tcagcttcac ggcgggctcg
    31141 ctccaaacgt aatctttcga caatatcttt ttctttagca aattcgccca gaattttcat
    31201 ataagaagat tcgtcaaccg gcgtcattat gcttttacag ggaattcctt ctttctcaag
    31261 atattttatg atatcattat gagagatatt taattccttc gcaatctcga aaatccgttt
    31321 gggtttatct tttgggtcaa tactcatttg tttatttctc ttcctgaccg aaatcgtcaa
    31381 tgatgtcttc gtcggataag tctgtctctt cctcaacggt ttgtttttct ttattttctt
    31441 gtgcctcttg cagggcggct tcgcgctgag ccttggcggc ttcatggatt gtagcaactt
    31501 cggctttccg cttttcctga gcttcgatga ttatcggttc aagtttatcg agggttttct
    31561 cgcctaatcc tttgatactc aataactctt cacgaggagt gtctaaaaat tcttcggcag
    31621 tttcgatacc ataactatat aacaatttcc agttatgctc agtaattcct tcaatatctt
    31681 ccagataaag ggtctcggat tccgttaatt cgtagtcgga ttttttaata gcgtcaatcg
    31741 tatagccggt aacttcggat gccagtcgta tattttgtcc cattttaccg atagctacgg
    31801 aaatttcatc atcggcaaaa atagccaaag cggttttctt ctcctcatca atttccagtt
    31861 cgagcggttt agctggtgaa agtgcgcgcg taatcagaat ttcaggttca ctactgtaat
    31921 ttataatatc tattttttca ttgtttaact cacgaacaat cgcctgaatg cgactacctt
    31981 taataccaac gcaggctccc acagcatcaa ttcgtcggtc attggattca acgataacct
    32041 tactgcgatc accggcatgg cgagcaactt ttttgatttc aatcgtctta tcataaattt
    32101 ccggaacttc attctcgaac agcttcacca gaaaagattt atcggcacgc gaaacaataa
    32161 tttccggacc gcttggtccc attatggctt ccttaataat ggctttaata ttctcgccgc
    32221 gacgatagcg ttcattcggt atttgttctt cgcgcggcat ttttagttcg gctttaccga
    32281 ttaagataaa aatggcgtca cgccgaattt gatgaacatc gccaattaca atttgtccaa
    32341 tttttttggc gtattcttcg taaatctggt ttttctccac tcggacaatc tgctgattaa
    32401 gaatctgttt agcgtgtgat attaaacgcc gtccaaagct catcgggtct atcacctcaa
    32461 taaacggatc ccctacttgc agatctggtt ccgtttttcg ggcattttcc aaagtaattt
    32521 cgtgcggcga accagtggga ttatctacga tcgtcatctg gtgataaatt tcaatttcac
    32581 ctttatccat attgacgatg acatcaaaat tttcggtcga gccgtatttc ttttctatta
    32641 aggtcagaaa tactttttca attatgtcac cgagttcatt ccgatcaacg tttttttcac
    32701 gagcgatctg agaaaaagct tcgatgattt cacggtttac caattttgga agtctcctat
    32761 atgttttacc attgaattat tactttggcg aaatcaattt cgcccatttt tagggtatga
    32821 gtaccttcag cattcgctat gacaagttgg tcttcatcaa ccgattccaa ttttcctttt
    32881 agcggtgact ttagattctc actatgatgg aatacgttca tttcgcgccc aagattacga
    32941 tgaaaatcac ggaaatttct caatgggaaa tcaattcccg gcgaactaac ttccagctga
    33001 aaattatcgg gaaagagttc attaaatgct acatcgtctc gcaccgcgcg gcttatttcg
    33061 gcaatctgat cgagggcaat tccgtgatca gtagtaatta aaaccttaac aaaagtacga
    33121 tagccctgac gattaacttt aaagccaaca acataagcat cgaatcgttc tacgatctta
    33181 tctacaattg ctcttgccgc gttcttggta gtctctaatt ccatgctaca cttctttcaa
    33241 actaaaagtg ggctttcaaa cccacttact atgcggcaaa tttaggtaat ttaggcttat
    33301 tattgcaact tattttagtg ccaacccctt gcgagtggta atttgggcta ataaaagctc
    33361 agctgaattc ttattaacgg attcgggttc gtcgttaaga attatgttta cgatttcttc
    33421 cgccttctca agattgttta cttttaaata attacgcgcc gaatttagga gtaattcgtg
    33481 cttatgaaac ttgtgtggcg agatttttcc tcctttttca aaatactcgg cggctaatgc
    33541 aaagttggaa tcttgttcgg cacaaattgc caaaccagaa taagctccag ctgttaaaaa
    33601 tttgtcatct ttataatcat caatgtactt ttcaaaattt actcgggcat tttcataatc
    33661 atctttagct aaataacagc gtgctaggag aatagttgcc atgccagaac tacgtgtccg
    33721 agaatacttt tcaatcaaat tttcgagtcg aagaatggcg tcatcgtaat cattgcgggc
    33781 aaatgacatt tcggccagtc ccagttctcc cattgccgcc gtgttggctt ttttcttgga
    33841 atttaccagt aaaatagtca gcaagacgac gaccaccact ccgagtaata tgtaagatac
    33901 taatttgcgc tgtttgcgga ccagattttc aatttgtgag attttttcta agaccgggtc
    33961 ttttttcaat tctttccggg ttatcttctt tttaggtttt aacatctatt tactcctgtt
    34021 gtaatttttc ctgtaccctc gccgggaatt gaacccagat caaaggatta ggaatcctct
    34081 attctatcca tttgaactac gagggctcaa aaagcgggta aagttatatt tatagacgat
    34141 attttgcaag ctacaataaa gtcaacgagg aaataataat gctgtatcta atcaataata
    34201 gtttctgaat tcgttataaa taattcggcg ccactctctc atgatttatc tatttcttgg
    34261 caaaatatta atttttttca ggttctggtt caaaatttca accgattccg gtgaaattga
    34321 ggtttcggta tagatttgag atatcagagc cggattattt cttaaacgct tataatccgt
    34381 ttctcgtcca gttattatat ttcccagtgc ttccttaatg cccataccga ttaaaatttg
    34441 gctgttacct ttccgaaaat aacacgatgg acgattatat cttttcggga aaagcgccag
    34501 aataaaaccg ccatattcgg ggaaatattt tagcataatg ttcaaacgcg gctcattttc
    34561 cctcattccg agtgccggta tcagactgac gtaatccggg acgtttttaa ttcccttgat
    34621 attttggctg acgtccttta aaagtattaa ataatggtcg aaaaatttaa gtaaagcctc
    34681 agcatttttc gtgactacaa tatggaccgg tcgcagaaag ttttcaatat aaaatacctc
    34741 cagctcgcca gattttagaa ttcttttgag acgtgcttga ccaaccgatt gcccggctaa
    34801 tagtttctgg atctggattt cgccaattag tttatcttta taaccggctt gaaaatggaa
    34861 gtgggggctc gatgcaccgg ctaatattcc attaaagtaa atcgaaaaat gatacagagc
    34921 gcgggaaatt tgaatcatat cattaaatcg ccccgttatc aactgcttat catgctttag
    34981 ggcggctatg gttaaatcac cgggataagt gattccggga ttggtcagga taataaattc
    35041 ttgttcatcg ccaactttaa ttattatccc acgctgtccc cgatcgagtt tacatagctc
    35101 acaagttttt ttgattgtat ctgtgttttc gccaaatgaa ctggagacca gactcggccg
    35161 acgagtttta ttcagcatca catgtactgg aatcgttttt acgattaatt ccgttatcgc
    35221 tacctgacta taaatctgca tatttttaat gagcttggga taaccttttt cgggatcgtt
    35281 ttgatggagg atgagagctt ccgctctata attatcgtca actttttcta acttgatatc
    35341 taactgctgt aaataaaccg ccagttcttc atcacgtaaa aatcttttag taacattcat
    35401 tctttaaaga ccttgttctg ttctatgaac cattcctatt agcctatttt ttaaataatc
    35461 tgatggtaag ctacgcaatt atgatttcag aggcaattat tagaaagtct tagtgaaata
    35521 atcaagaatt tttatgttta ctaaacttaa aataggtgtt aaggaacatt aaagaattac
    35581 agcggattgc ggcgatttca aataaattgg tgatttcgaa taaaatatca ataactgata
    35641 ctcaagcttt ccatcacaaa taatcttttg gtattagtca aaatatcctt ggcagtattt
    35701 tttggtcgaa atccaaatat tttttggaaa aaacccggaa gataactttt aacccgcctg
    35761 aattgcccat ttggcgggca agccgatgat ttttagaaaa gaaaaataaa tacccttgcc
    35821 ccgcccttct cgtacacgtg catttgaaat tcaactccca aaattctctt tacattatca
    35881 aaataatttt gtatcataat tatagattga ggttattcta tagataaata ctatttattt
    35941 taatactaca atgtcaagag agatttcacc aataagcaaa aatatcaaaa agttaaggaa
    36001 acaaaaggac ttggcgcaag accggctttc taaattggct gatatttcct ataacacggt
    36061 cataaagctg gaatcgggcg gtatcaccta cccttccatt ggcacgcttc aaaaattagc
    36121 ctaagctctt aatgtttcag ttgaaaattt attaaagtaa tattaaattt gaacatagca
    36181 gaaatgaaga aataattatt tattttactt tagctttagg gggtatagat gaaagatgat
    36241 gaaataaaac aaattgttaa aacaaagtat tctaatattg caaaaggcag ctgtggatgc
    36301 ggcaatgttt cctcatgctg cagtaattca ctttcgacta aacaaatatc agaaagcatt
    36361 ggatattctg aagaagagat caattctgca tcagaggcta atttaggact tggctgcggg
    36421 aatccagtgg ctctttcaac cataaaagaa ggtgatgtag tcctggattt aggaagcggc
    36481 gctggttttg atgcttttct ggctgccaaa aaagttggga ataaaggcaa agtcatcggg
    36541 ctggatatga gtgaagagat gactcataag gcaaaagaaa atgctaaaat gtatggctat
    36601 caaaatgttg aattcaggtt gggagatatt gaacatattc caattgaagc aaatacaata
    36661 gatgttatta taagcaattg tgttattaat ttagcaccga acaaggaaaa ggtttttagt
    36721 gaagcatatc gggttttaaa aagtggtgga aaaatgtttg tttcagatat tgttttgtta
    36781 gaagaattga cgcaggaaca aagaaacgat aaagaattaa ttgcaggatg cgttggcggg
    36841 gcaattttga aagatgagta tattaaacta attcaaaatg caggatttaa ggttaaagtt
    36901 ttagcagaag acaaaaaaat tagcaaaaga caatataatg gaatcaagtt agagagttta
    36961 aaaattgaag cagctaaata aattgataaa taaggaaaca tgcgccaata agtagcacaa
    37021 gtaataaagg catattaaag cactaaggta tatgaaggaa aagagtccat aaattagatg
    37081 atgttgttgc cttcgtttaa cttcatagga ggaaaagaga gaatgaagag gctaagtcac
    37141 tttcatctaa tgaaagttaa acaaaaattt ttcttaattt tgcgggtggg ggcttttaat
    37201 ggacttaaaa aaaagattta taatgaaaga gataatggag taatataatg accaaagaac
    37261 aaaaatttta caaagcatta caggatgtat accagaaaat ataccttgaa agaactccga
    37321 aagattatca atgggcttgg tttacgctgg cggcgagaca acaaccctca aaaaagctga
    37381 aatcgtaaat tggcggagag ggagggattt gaacccccgt cccgataagt cgagaaacgg
    37441 ttttcaagac cgccgcattc aaccactctg ccacctctcc aaatagcggc gttaatttaa
    37501 tatttttagc gaagtttcct caaaatattt tcagataggt accacttatt ttaccaacaa
    37561 accaaacaat aattaaaagg atactatatt ttgggattat ttaatattcc aggacttgta
    37621 gggatttttt atcaaattgg aattgaagta acgcggatca ccggtcacct cagctcccac
    37681 ccagtccggc ttaataaatg attggttttc gtcggcgagt tcaatctcag cgattactaa
    37741 tccagcatta tctcccagaa attcatccac ttcccagata aatccgctat gcttaactcg
    37801 ataccggatt ttgtctaaaa tgggcttttc gcagatattt tcgagaattt gttcagcatc
    37861 ttgcctagga atttcgtatt caaactctgg tcgcacggct ccgacgttta tgcccttaat
    37921 tgtcaaaaat gcctgttgac cgactgttcg aacccgtacc actcgctctt tgactgtgct
    37981 gagataaccc tgacgataac gtacaccggg gctaaaagta cgataatcgt tggcaattac
    38041 cagaaatttc ctttctattt ctattcccat attagtgcct tttcattttt ttcaaagcct
    38101 tgatgtaatt ggtattgggc aagttatcca attcgagcaa ttttagtact ttttgcacat
    38161 tttgcggttt ttcgctttca acggcaatgg tcttaactga ttgattgcca acctgcaatt
    38221 cggataattc gacttcagcc ccttcgagag tgtaacacct tcgcttcttt ttaacatcta
    38281 ccaaacttag tttcggattg gatgcgacga tttcatttaa aaattgatga tgagtataat
    38341 gatcgcgcat cgccagcggc ttacgaacat gtaaagtgcg cagtatgaga cgtacgattt
    38401 caaaactaag tggaaatgaa gcctttagaa ctggtttcca taattccaat ccatcgggat
    38461 tagtttgttc gataatttta atatccatca acgaattgcg gattttgcaa ttttcattgg
    38521 aattttttga taaaatgtaa atttcttcac tctccttgat agtatcgtat gggagtgaac
    38581 tcaaaaattt ttccgctttg gggaattccg atccgaaagt gcgccattcc catcttggaa
    38641 ttactctgtt attaatttgg ttagcgatat ttttctgaat tgaatctttc ataggtattc
    38701 ttggttattg gcttacaatt tgtttgatta atgtaattaa atagatgaga attaaaaaga
    38761 gatagacttg gatttattat tcctgatttt atgattttgc cggtcacttg aaggcgatcg
    38821 gcatataaat tggctttttt cgattttgaa gtttttactc ttccatgtag tgactttatc
    38881 accgacttat atttcctatt tttcagccaa attcataaac aattaaattt agatattacg
    38941 aattttacct gccaagaaat agttctgata aacatatttt agaggtaaat ctaagttttt
    39001 tcagagagat attttggaaa atttttgcga ttttagtttg atggggacta tttggaaccc
    39061 tggcatccgt ttttgacgaa ttcatcgaaa ctgattccag ttttataaca ttcctgatgg
    39121 acaaatttca atagattaag gaaaaggtct ttttcgatgt agccgggaat tacttcgatt
    39181 acttccattt ctggggtaat aaaagcaatt gacggaaaac cggtcacttg aaatgctctg
    39241 gtcaattgac ggggggtata agttttacct tggaaaacta acttttcctt gtcattttcg
    39301 gcgttgatcc gaattggaac aaaattatca aataaatatt tacttacggt cggcaccgag
    39361 aaagtctttt gatccataac tttgcaccag ccacaccagt cagtgtagaa gtcgagtacg
    39421 atgtgtttat tctctttttt agccagagca ataccctcgt taaaactata ccattctttc
    39481 cctgccgaaa ataggctgga agctaaaagt agaatcaaaa ttaccgacat ccatttattt
    39541 ttcattttat ctccattttt ttactttgat aaccaattgc ggtattggtt gcatagattt
    39601 tataaaatca aattaataaa aaatatagga aaagtatttg tccaagaaaa ttggtacaac
    39661 tcgcgctttt agcgttttta ggtctcaata ttaaataatg ctgtcgccct ttcgaatata
    39721 ccgagcgtat aggcgatcgt caacccataa ttcgtaaccg gaactctcgc ttgtttacaa
    39781 taccagatgc gtgacaggac ttccctccta ttcagcatac aactaccaca ttgaatcact
    39841 agcttatatt tactcaaatt ctctgggaaa tcgtgtccct ggtagtgagt aaagttcaat
    39901 tgtccaccga catactgatt taaccagcgg ggaattttta cgcgaccaat atcgtcagtt
    39961 atgggatgat gcgtacaggc ttcagcaatt aaaatttcat cgccgggttt gagtttgtca
    40021 atcgttcgag ttccagcaat atattctgcc aaatcgcctt tgtaacgggc gaataaaatt
    40081 gaaaaagatg ttaatggtat atctgctggt gtttcggcgg caaccctcag aaaagcttgc
    40141 gaatcggtta ccacaatcgc cggtggttgc tttaaattat ttaatactga ggttaattca
    40201 ttttctttga tgacaatgca accagcatta ttatccaaaa ggtcacgaat cgtctgaacc
    40261 tgtggtaaga ttaaacgccc tttgggtgct tcctgatcaa tcggcacaac taacacagcc
    40321 atttcaccag gcttaaccaa atccccaata atcccgatgg aattaaagta atcagccgga
    40381 gtagtatcga tcaaaatttt atgcaaatcc agcacgccga aaccatcagt tgcgatggta
    40441 ttgatgtacg gaatattttg ctgttttaaa tatgcctgca gattttcaga aggctgtgat
    40501 agatcagatt tattgaaggc aactactgtc ggaatgtgcc gattttgcaa ttccttcagc
    40561 agatcatctt catatttatt ccaatttttc ccttcggcaa cgataatccc gagatctact
    40621 cgttccaata ttttacgcgt gcgcgcaata cgctgtccgc cgagggagcc ttcatcgtcc
    40681 actccggcag tatcaataaa aagcaccgga ccaagcggtg gtaattccat tggcttttcg
    40741 actggatcgg tggtagttcc agccacatcg gagacaattg agacatattg acttgtcagc
    40801 gcattcatca aagacgactt accgacattg cgacgaccga aaattccgat ttggagacga
    40861 ttgcttttgg gggtgctttg catattttca atttcgccag actttggaaa tgaaatgaca
    40921 ttttggacta ctgtgttctt cagtcatttt cgatttccgt tagaatttgg gttagtgctg
    40981 taaaaatctc gttcattttg tcaggattaa tttggtcgtt ttgtttatag aaaattccat
    41041 atcgcgaagt caattggcgg cgctgaggag caaattcaag gttccggtca ccggctacat
    41101 acgaccagcg cttgccataa attttacaaa agttgacttg caaaccatat tcttcttcca
    41161 gttttccggc aattgcgttt agtttatccg gtaggttagt ctggttcgcg gctgattgta
    41221 aaatttcttt agatatagac atcgcgttcg ccgctggcaa cttgctgata gagtctctcg
    41281 aaagtttcca gcattcgccc tttctggaaa aacggcatcg ctttaatttc ctcgatttct
    41341 ttttgaatca atcgttcgcc aaccgcttta gtttcaggcg aggcgtaatc gtcaaggtat
    41401 tcacggaaag tcaagacggc attcgcctta cagaattttc cctcgacaca accttttaac
    41461 aaattcataa ttttgccacc ggtgcggcca caacgatagc cagcggtaca gaaagaagtg
    41521 atcagacctt gttccgccaa ttcacggacg acttcatcga gactgcgtgt atcgccgaga
    41581 gtaaattgtt gttcatcaat ctgttggtca gccttttctt tttttgccag ggcttgcaga
    41641 gcggaagtat agccaccgat gtcaattcgc gtggaagcat ccgtctgggt acaaccgact
    41701 ccaataactt cgcgacgcat ttcagcactt tcgcgcgcgg tgacaattaa tccggtataa
    41761 ggcacggata agcgtaaaac ggtaaccagt ttcttaaaat cggcgtcact aacccgccat
    41821 tgcgaattgc gactgaacca ggaaccgctg gcaggcgtca agcgcggaaa tgaaatcgta
    41881 tgcggtccga taccgaaatg ccgttcgagg tcaatcgtat gatacagcaa gcccataact
    41941 tcgaagcgcc aaccatacaa tccgaaaagc gccccgatgg ctacgtcgtc tattccagcc
    42001 tccattgctc gatgtaaagc atacaaacgc cagcgatagt tacctttgat agtattaagc
    42061 ggatgcagat tcgcataagt cggtttgtgg taggtttcct gaaaaacctg gaatgtaccg
    42121 attcccactt cccagagttt cttcagatcg gcgatactca tcggagcggc attgacgttg
    42181 acgcgccgaa tcgcactaaa gccggtttta accgggacct tgacgtcgta aatcgtgcga
    42241 atggtttcgg caatgtaatc caccgccgag cgcgggtgct cgccgaagac tacgatcata
    42301 cgcttatgcc cttcgttaat cacggcttcg gtttctttgc gaatttttcc catagttaaa
    42361 attcgacgtt tctcttcttt attttcatag cggaaaccgc aataagcgca gttattaacg
    42421 caaagattac tgcagtacag cggggcaaag aatacgatac gattatcgta aactttccgc
    42481 ttaactgcca gaccggctaa gtacatttct tcccagagat cgtcgtcagt aacattcagc
    42541 aatgttgccg tctcttccgg ttcgagtcgt tcaatctcta aagatttcgc taaaatctca
    42601 cgaacgcgag atttatccgg attggcggcg gatttaagtt gagatgtaat gacggtttca
    42661 tcaataaaat ccttgccttc tatgagatac ttttcgatct catcgggctt gattactttt
    42721 ttcgtccatt cacttacttt gacattttgc atttatactc cattgaaaaa atcatttact
    42781 tagataaaat attttgggat tcgtgacgcc gaataagatt cggtgaaatc cccttgctaa
    42841 gactgcggtg aaatcccgcc tcacggataa tttccaatac ggctggtatc gaatgttcag
    42901 aagcatcgct atcggcattt ttgccgggat aaatgttgta gagcaatttg actctctccg
    42961 gagttattga cggcataatg acattcattc caatttttag agccgcaaga cgagcgcctt
    43021 tttcggcaca ttccatagca ctcgtcgaag gcatattggc ataggggttc agaatgcgca
    43081 ccagcgccag cgttcgcaga gtcaattgga ttgaaccatt cggcttatct ttcaaaggtg
    43141 tctgaggatg agccagaaat ggaccaatcc cgatcatatc aagatcaagt tcagtcaatc
    43201 cgataatggc atcagccagc gttgccaaag tttcaccggg caatcccacc ataattccac
    43261 taccgacttc gtaatccagt tctcgtaaaa ctttcaggca attcagccgg tcttcccatc
    43321 gtttgcctgg ccgtaaagag ccataatttt cccgattcca ggtttcgatg cgcaacaaat
    43381 accgggtcgc gcctgccttt cgccataatt gtaaaacttc cggagtctgt tcgccgagtg
    43441 aaagggtaat cgccagatca tatttatcaa taattttgcg gattaaatcg gcaatccatt
    43501 ccgctgaata gtaggggtct tccccaccct gcagaacgat cgtcggaatt ttttcattag
    43561 cggcagtttc cactgcggct ataatttcct catgcgataa ccgatagcgc tggacggcgc
    43621 gattggaacg gcgcaatcca cagtagtagc aattctgccg acagtaattg gtgaactcga
    43681 taataccacg aagataaatt tcatcgccga aaaccgcccg ccgaatgcga tcggcttcat
    43741 tgaataacca attgtcatcc ttaccttgca aaaattctat gatttcggtt ttttgcatag
    43801 ttatttacta accattgaca attagaaata gccaaataaa aattttagat ttcacacttt
    43861 tgatatcttt atccacaata atagtttcat aatcttactt tttacatcat cactcaaata
    43921 acctttagtt cacaatttta acaaaaataa tgttaatcct ataaaaaatt atttagaata
    43981 attcaggtta atctaatggt aaaaacaaac cggcttttaa ttcacaagag aaaatactac
    44041 ctatcttgat gtcacttgat actatttaag taccataagt ttgcgagttt ggatattttt
    44101 tcccgctttc atttgataaa gataaatgcc actggttaaa tcagttgcat cgaagtttac
    44161 tgcataattg ccaggtgttt tatattcatt caccaaagta gccacttcat tgccaaggac
    44221 attataaact ttcaatacga catattgcga ggttgataat gaataacgga ttgttgttac
    44281 tggattaaaa ggattcggtt ggttctgata aagaaggatt tgctctggta ttatatcatc
    44341 tccacccaca atccctgtat tagttgaaac accataagct ttaacgcaga gatcatattt
    44401 ataatcggta ttatctccta ttgcgagcca gttattattc cctgtactac taatccagca
    44461 tttacctgtt tcgataactg ggtcagcata atcggtaata aacgtctcag ccggaagaga
    44521 atattgagtc tcaggagtat tgtattttac ttttatgtaa atatcatcac cactgtttac
    44581 ttctatcgaa tcgggcaaat caaaggtata gtaaccggcg taatcacaga ttttatctcc
    44641 ggttgaaccc aaaagagtcg aaagagatct cccatcaaaa ttatcataaa tataaaaatt
    44701 cactgtcgaa ttggaagaat gcacccaagt tcctactttg gtaatttttt ggtcagtaga
    44761 tataacaaat tttaccagac cataatcttc ataatcaggt tgatcaaggg agttatcaac
    44821 ccaaccaaac gatgtaacac atcctaattg gtcgtagtaa tgaacattag cgtttggatt
    44881 gtaatccata cgatttggcc agaaagccgg gctcgtattt actttggtat cgttatagga
    44941 aatgtagaaa aagccatttt ccccgaaacc tgaaccccag ctatttctta taatccaggc
    45001 tcctgtgcca ccagcagtct ccttactgtc atcccatcct accagcagga ctgcatggtt
    45061 ggtactagtg tccacataac tataataggt atgattttgt gaattataat aagtattatc
    45121 ccagaacata tcggtatata cagcgccata agtatataca atctgtttaa ggatatttat
    45181 gtcatccggt aaaaatcgag cgtcgggaac ataggcttga gaacttaaac ccgaaacata
    45241 aatactatct tttacattgt aagggtcatc aacttcggca attgggccat ttccgcgact
    45301 gaaataggca gttgccattt tggcattacc gccttcgcag gaacctaaaa gaaagccatg
    45361 gccatttcta aggttttgtt cagaaaggtc ataatccccc aattctaaag ctttccatct
    45421 tgattcgatc gaaccgtaag tcgcaaatgt ccagcaacaa ccgcaatctt tctgatcctt
    45481 taccgaggtt agtttacctt cctctcgcaa gtcatacctt tccggtaacg aaacttgctt
    45541 ctgaagatgt atcggaattt ctgtttcaaa tgtcaatgga tgcggaatat agccaagggc
    45601 gtatccttcg ggcgtaaatt tctttacctc accctttttt accatttcta tataattgat
    45661 aaactccgag tcaagcggag tcatttgagg atttcgctcc tgcgcaaaaa tcacggaaaa
    45721 tatcacgcat aaaatcatag gaacgtaaac ctttttcatg caagctcctt atcggttaat
    45781 gtttgaccac aacaattagc tcaaattttt taaccggcgg catatccttc tcaaatggtc
    45841 ggcgataaac taactgtaaa aaggtctcgc cctcttttat cgccataaaa cgcattattg
    45901 ttttcccacc tgaacctaca atacccgacg gtactttatc agcctggtat tcgatgccaa
    45961 tgttttttaa tacactggta tcgttggaag ctacttccca gcgataaccg gtagtcggat
    46021 tggcttgcag gacaatttcc agactatcac ccttcttcat ttctaatata ctgccggcac
    46081 cactctcact tatttttatc gtcaccggct tacattgaaa tgaaaaagcg agcagggaca
    46141 aaatcaaaat aaattttata gtagttttaa accttaacct cgatcttaat cttacctaat
    46201 ctcccgccat cgcccatcag ttttcggaac gataggagaa tgttctctaa tataatcaat
    46261 catcgcttgc cgcagaagaa tgccggtgta attgattcga tccggcgtaa tggtcgtcaa
    46321 taggtcataa ccagaattcc cttccgccaa ataatccgta acagccagag tatattctcg
    46381 ctcatactcg ataggttttc cgccaatgtt gaattttata actcgcgaac catcgggttg
    46441 attgcgatct attacgattt cagccccgcc cacaagcata ccttgattgc tacctgaaac
    46501 gcgatcttcg actaattctt tcaaaaattt gccggttacc ttcattatga cgatacgatt
    46561 gccaaacggc attactttga aaacatgccg aggagtaatt tgcccagcag gcaggtcggc
    46621 tctgactccg ccaaaattag aaaaagcaat gtcggctttt gtactggcaa gcatggcgtc
    46681 agccatcagg ttgcccatcg gtgattcgcc gctactcgag cgatagattt gtccggtcgc
    46741 taaaccgatg acctcgtcga atccggcttc ggctttttgg acccagaaat caatcagtcg
    46801 tgtcagactg gtatccggca aaaagtcatc ttcaaacaag gtgaatatcg cgccgtcgtc
    46861 agattcataa tcgtaacccg ccagagttcc ggtctggcga tgaatataaa aattcacatg
    46921 tcccagatta ctgccattgg cataattttg gaaaatcagg gtatgattaa gcggatcttc
    46981 ccacggctcg taaaagccgc gatgcaaatg accagaaaac ataatatcaa tccccggaat
    47041 ccgatgagca atagcctgcg cgctggtatt ataactttca cccagttctc cgtcactgag
    47101 atcagccatc atttgcttat agccttcttc cggatcgtag gctaaccagg cgtgcgtcag
    47161 aacgataacc gtattggcac cttgcgcctt taattccggt aaatagcgtt tgattccttc
    47221 aatctcaggg cggaaatcca ggtcttcaat gtgttccgga taactcatac tgggcgtcgc
    47281 agaaagcgtc gctccgatga tgccgatttt tattccctga atttctttga tgatatacgg
    47341 cttaacaaat tcagccaatt ctccggttga tttgcggtaa aggttagtcg ctagaaatgg
    47401 aaaattagct ttctccgcca attttttcag atttttccag ccattatcga aatcgtgatt
    47461 cccgatagtc agggcatcat agccgacggt attcatatat tcgataacgg cttcgccctc
    47521 cgaaaccgtt cccagcggcg tcccctgata aaaatctccg gcatcaacta ataagaatcc
    47581 ccagccattt tgggcggctt cctgacgctt ttgtaaaata taacgtcccg cgactgctcc
    47641 gccacccagc atcggcggaa aatcgggatt aataaaactg gcttcgctcc gatcaattcc
    47701 accgtgcata tcattggtaa aaatcgttac gattttcaag taatctttag cgggaaattt
    47761 ttcagccgcc aaaagcgtct ctgctattag aacgacaatt aaaataatag atgatctttt
    47821 gtatttcata gattaaaaat tgtacctcaa ggttgccatt ccaatcagat aactttcgcg
    47881 aacggtatca aattcgaccc gcttttctac cgcaattgga aaaaggtcgt gatacttgta
    47941 atcgcaagtt gtaaaagctc cggaaatttc gagattgaaa ttaccaaatt gataagcggt
    48001 gcccgccgtg aaccaattgc gattcatctc ggcattgatc ggattaggat cgtgataaaa
    48061 tccgaaccgc aaaggcatac cggaaaagaa aatatgttcg atgccaagat ggattttcgt
    48121 cacattgcga taaggaatcg gcacgtccga aaaattattc ggaatatcat tcggattggc
    48181 gtcactccta aactgatatt tagccgaggc atctttccaa ttctgataga cgatttcgaa
    48241 aaacaattgt gtcggaatta tatttactgg ttttaaacat aatccaaacc gcaattcacc
    48301 cggttgatga taggtaactt tttcgatagt aaaaaccgaa tcaatcgcca gtaccggcag
    48361 agtctgggta gtgtcattta ctaaataaag taaaccgcct ttctggactg tgttgtatgg
    48421 taatcggtaa gaaatcgcaa ggctgagatg cttggtaacg tcaaatgcca tcccgaaatt
    48481 gccggtaatc gccgactcga atttcggttc gacgacgtag gaaatcgtgt gatcggaaga
    48541 ttggtagtcg ctgggcttga tgacctgaat ctcatatttt tgctcggaaa aatccggggt
    48601 aataaaattt aacgccgctc ctaaccagag tgatttccat ggatttactg ccattcccaa
    48661 cgaagtattg aaaactgaat agcggttcga tagtcggtta taacccacca gcggatcgcg
    48721 attgtattca ccgtacaacg agcctctgac ttcctcttca tactggaaat cactcagatt
    48781 ggtatgattt aacagcattg ccaaccggat gcgcgccaga ggtttggtgt taattccaac
    48841 ctgaaaatct ggaaaccagt tctggttcaa cacgtaagta ttgtcagcca aaaaatcgcc
    48901 ataactatcc tgcaccggga aactgcgttt ttctttaacg gaaaatgcat ttaatcccgc
    48961 ggttagcgac aatttttgtt tgctgtatcc cagcaaagcc gaatttgtga gtaaagccga
    49021 agcgcttccc ccatcacccc agcctaataa gccgaccgct aacgaccgcg cgtcggctac
    49081 cgacggtaaa tcgccaatca acgggctaaa gaaactctgg cttaagccag ttgagctaac
    49141 gattaacaca aatgtcagaa tgaatatttt ctttttcatt taaaacctat aatcaagttg
    49201 gattctgaaa tccgaataaa atttatcata atatacttcg ctgacgactg gattggaact
    49261 gtccgcgcca atatcaatca ttccacccac tacattatct aactggttat gcagatcgaa
    49321 actgtatttc aggcgaagcg aaagatcgcg cgacagccgt gagaaaactg taatccagcc
    49381 gtgaagcgcg cgcgtgtcgg tattgaacac tcgaaaatcc gtgtcttcaa aattccagag
    49441 aaagccgcta taggtcatca ccgaaccaat cagtttgagt cgttcattga cattatgagt
    49501 aacggtcaga ccaagcgcat cactgggcga accggcattg ccgacataag aagtttcacc
    49561 ggtttcggca ttaataacca gtcgtgagcg tgtggtaaat tcggtaaacg aaattgaata
    49621 taacagtttt acctggttat aacgcgacat ccgcatagtg gcttcgaggc gagtttcatt
    49681 agactgatag aaaaccggcg tgagagtatt ggtcttatcc cgatgttgcc atttctggcg
    49741 aaccttgaac cgatacttga aaaccggacg atattccaga ttaagcacgg tgcggtaata
    49801 ttgggcgtta tcggctacac gagtccaaat atcctgttcg gcggtggcga ttaagctgcg
    49861 gtgcaattga tagcgagttg atagatacaa tccgcgttct gcctgaggct gagccgtagc
    49921 cgaataaaga taggcgaggc tggggtcttt gagataatat acatcctcgt aaatcgtacc
    49981 cttgaagcgc tgataattgc tataactgcg ctggtaagga ttatcgaagt ccaggtcgta
    50041 atcccgatag agcataagga aattaaaatt atcaaactgt aaataaccag acaccaccag
    50101 cgcactcgga tcgccggaac gatcgcctaa cttcaagtct ttatccagtt cggcatattc
    50161 accttgcagg gtcaaattac taatgaccgt cgaaaattcc agtccgtaaa ctcgccggat
    50221 agatttggca gatgaccaga ttgaagatgg agccgatgac tcgtaagagg cattaatttc
    50281 cgcatcggcg gaattgccga tttgggttaa atatttgcta tagccggaag ggtcaagcag
    50341 tgactcggta acttgtaaat ctaattcccg gtcatacaga ctctggtaaa ttgtcaaacc
    50401 taaatacgtt cccggcacaa aagaatattt ggcgttgccg ccgatcacca tttctttgac
    50461 ggcattgatc atattatgcg gaatattgcc gtacaaaccg tgtgacatcc gcggatacat
    50521 cgtaattaga gtcgaaaacg aaccatcatc attgacaact gcgtctcgat tattgatgga
    50581 gaaaaatccg gtgccaatca gattaccgat ttgggcttcc gcaccaatac cacgcaagcc
    50641 gtattgcgta atgcgcgaag aattgccact taaaccgttc agccgattgc gaaaaccgta
    50701 tccggttttg cgcggcataa aaaagtcggt agcttcaaaa gtcacccctt gcccaaaacc
    50761 agccaaataa tcaccgatta cgattttgtt caattttacc gcgccgattt caacgttatt
    50821 tatttcgaaa aaggctttga attgcggaat tttggtaccc tgataataaa tcgtttcctc
    50881 tcccaaattg cgcaagtaag aaccttcgag acggaacttc gtgccccagt gcaaacgcat
    50941 tttgtaataa gtgtccagcg gctgatccag ctggacgaaa gcggcgtatg aatcggcgtc
    51001 atcgctggga gtctgactga gggtaatatt cttgaccacg ctcgaaaaac tgccgccgaa
    51061 cctgtgaacg ttatcgtcat cggtatagcg aataaaatct tccaaattgg aaaaaccgta
    51121 ataggtcaac ccctcggtac tgcgtaaatc ggtgcggttt ttaatctcgc cctgtttttg
    51181 gcggttaacg atcgccacgg cgtcgcttgg agctagtccc gctagattcc gcaattcaaa
    51241 atagctaagt ttgttgatat taactggatt ggagagcata tcaatccagt tattgacggt
    51301 attctcactg gcaccgtcat cggaaatcca ttgctcgact ttgtaataat tatcttcgat
    51361 gcgtttttcc gattcttcca gaaaaaccgg cgagatttgg gttagttctt tcagtttcaa
    51421 aaaagtttca tggtcaatac ttttgatatt ctttaagtcg aatatactac taaatgggcc
    51481 aatgtattcc cgccagtata gaatatcttc caattgttgg cgagtcaacg gcaactgcgc
    51541 cagttcgact tcgtcggctg tattgaggtc aattttcgat tcaagttcga cagcaccaac
    51601 aataatgttc agagccatta taaataggat caatagccaa caatactttg aatgcctcat
    51661 aaatttactt ttcatttttc ctgaatgaat agcccagact gaattgatga gtttcgggta
    51721 atagcgggtg actgatataa gcgtaatctg ccagaatgcc ccatttctcg attccgaagc
    51781 caaagccgat gcggttcggt tcggtattga cgccggttcg caggactaac cacggtacaa
    51841 tacggtattc caatccggca cgatactgag tcttatccgt tccgattttc tggtaaatca
    51901 caaaattggt cgtcaatccg taataaggtt catagcctaa accaattgcc acactcctgg
    51961 gaaggcgctg agcgctcaag gcactcccca tttccggcga attgatattc ttaacaaata
    52021 atccgatcca gcttctttca tgcaggctac cctggatgcc cagatcaagt ccaaaaccaa
    52081 acccgctacc caaatccatc ccgtcgctcc cgtcgccgga aacgcccgct gaatgaccat
    52141 aatcaagttg gtagagattg agagtatagc cgagcgatag cgtcgaaaat aggtcttttt
    52201 gcaggtaaaa accgtgcgac aggctaaaag ccgtttcact cgtcaaggaa ttgccggaat
    52261 aagaggtcga actgccttga tagccaattg ccattgtgcc gactttgccg attggcatag
    52321 cgataattgc cacggcacta ttataatacg aaagtccata aggtctgtat aaaccgccga
    52381 ctaattcgag agcggttaaa cgacacaaac cggctggatt gttgagttgt gcccaggctg
    52441 aaccgatatt tgctaaattg gatgaaccgg tagcgctgat tggggcggcg atttcgttca
    52501 cctgggtgaa aacctgagcc cagagtgcga tcggaattat aataaataaa aaagcaatta
    52561 acaggtgctt aaaagttctc atcagaattt cactcctact actaccggcg caattgaaat
    52621 ctgggatttt cccgtcgaac gattggtcgt ttgtagatgc aaaatataat taccgggcgg
    52681 caccagctga ttcaattcat tgcgcccgtt ccaggtatct tcccttttcc aggaaacggc
    52741 aaaatagttg tccaccagcg tcgtgatgta ccgacctgac aggtcataaa ctcgcacaat
    52801 ggcgcggcaa ttcgccggat attcataggt gtacttgatg acttcgccta atttcggtac
    52861 gaaaggataa ggtgccacgc tgattttggt gcgtccttct ccaacttcac caacaatgta
    52921 aggctcaaca tccgaggcgt aacccagcag gatttgcggt gcattattat aaataccgac
    52981 aaccccgcgc acaattacct gattgccgag ggtcaataat gaatcggtat attcattgac
    53041 gatttcgccc agcgaattat aaagcactcc agtcgtattc cagatgcgca cagtcgttcg
    53101 ggcagtttca tcttcaataa caacgttaca tccctgatta gtggcggtcg gattactgcg
    53161 ttccgctaca gtgccgtgaa tttcgacata actgccttcc caatcagtca aattattcgc
    53221 caattccgaa atatatactt tggcagtact cgttatcggg acgttttgag ccagaacggt
    53281 ataactatta gtccagttgg ttaattcttt gactccattg tattcggtca gttcgccggt
    53341 cacttcgatg gaatcgccct gcatcaggtt ggtaattgcg gaagcaaaga ggttcaatcc
    53401 tcttcccgaa acatcctgaa aataagccga ggttcggctg gtaataagaa tattcgaccc
    53461 gattgtcacc acacctcgca gagttacggt ttgaccgtta tattcgtccc aattttcgcg
    53521 gatggttttg atagtcagca tttccggtgg cgcttgaatt gtaatcatat tctgattaga
    53581 agtgctgatt agaccagatt catcttcggc ttcaatctga taataaagaa taccggcaac
    53641 tgaaaatgga ccaatggtaa cagaataagt atttccggta gtcagcgaca tttccacggt
    53701 accagtctcc gattgaaaag tataaatcaa tctaaccgcc gcgattgatc catcgtcggt
    53761 aacattcacc gaaaaagtta taacctgacc gacaagcgga ctactcggcg aataattgat
    53821 actggataat tttggtttgg tattgccgac ggcaaccacc tgccaggtgt cgtcgaggtc
    53881 ctcgccggtt tcgttatgac cggtaatgcc attgccgcct gatgatggct ctgagccttc
    53941 atcactaatg cgctggagct tttgaccgtc ctggtgggtc ggcgtaaaag tctgctgttc
    54001 gaccggcgta tcgttctggt aaccagcaat gccgctttta tcaacggcgc agattagact
    54061 gccccaaaga atataatcaa catctttgac cgtcggcgat gagccatccc aataaaacag
    54121 taccagtgtt tccgctgcat tatctaaata agctgtaccg atacctttcg tattctgccc
    54181 gtcgattgca tttagcaaat cgtctttaac cgacaagccg ggcgaagatc cgtaataatt
    54241 ttcataagcc gcggtggtcg tagccgcgat aatgatttct gattgcgcct caatgctata
    54301 acccggcgga aaacgtacta tgaaatccga accgcttcca ctccagaaat tggtaccggt
    54361 tggtagatta tagtaatatt tttggtttga tggatcagtg ccgtcggtaa gataataatt
    54421 actcaggtca actgcattat ccgtcgggtt gtaaagtttg atgtattcgg cattactcgg
    54481 ctggaggaca atctcggaaa tgacgacgtg atcctgcgcc gttaatagta aaaccggcaa
    54541 tagaatcagc ccaggtagaa taattcgttt tttcataggt tacttcaagt acaacatttt
    54601 ttgcgttctt acgcggtttc cggcttgcag ttgaacaaaa tacacgccgg atggaaactt
    54661 ggcggcattc cagattttcg cataacttcc ggcggtggca taaccgctgg caattacgtc
    54721 aattttctgg ccaagaacat tgaacacgac aacttcgtaa gcaccatttt tatccaactg
    54781 ccaactaatc gtggtcgccg gattgaacgg attgggataa gccgggttga gtttgaaagt
    54841 cagagcatta gttttactct ctttaatcgc ggttaatgga acaatatctt cggcgtagcc
    54901 gatcagggtt tgataagtac ttttatattt attgccgact cctctgaatt tataagaatt
    54961 gcccagtgtg atagatgcaa cgtcaattcc ggtagcgtcg ggaatccggg ctatagtcgt
    55021 atcagtccca gccgaaacgc taatattagt cgcctcatcg gaagtttcct gtttggttac
    55081 cattccggaa aactcgatca gtgtgccttc ccagtcactg ctattagcac cggcgaccgt
    55141 tacgacctga gccggtggca attcgttgcc actggagatt ttcttataga taaaattagt
    55201 aacttcaatg gtcgtgaaaa atttctcaac ataaccgact accatcaatt catcaccgcg
    55261 cgtaatatca gaatataatt tgccactctt gtacagattc aaaccgcgac cggacgcatc
    55321 ctggatgtaa gctgaagtat aatcatttgc cagaacacca gcgccgatcg tgacaattcc
    55381 tcgaatcgtg acaattttgc catttaaaga ctcaatgttt gtatgaacat tggcgattgt
    55441 ctcgggagtg ctattggcga ttatcagcgt ttgggtagct gattttccgg agttggctgg
    55501 tgactgatcg tccttgcaat aaatataaaa ctcgataatg ctattgccgg tctgaggcgg
    55561 tatattgccc atccagcagg tatcaccggt ttccttccac atcaccgcgg tgttgatcaa
    55621 actaccgggc gctccgtaat aaatcgttgc cgaagtaata ctccctacca ccggcgtaat
    55681 cgttgcgctg acggtaatct cgtcctccaa agtgataaaa tcgggtgaaa cagtaatatc
    55741 actgaccagc ggcgcattgg gattagtatt atgcgaaccg agattttcaa cattgtccgt
    55801 accaaattca atccattcac tttcaatata tgtatagcta ccaaaagtaa ttgtactgtt
    55861 acgcctcatt gttcgattat ctaatattgt atcaattttt gtgggctcac ctattatgtc
    55921 aacaatcaat ccgttacgaa acaaagccac agcatcattt ccattaaaat tcaaaggcga
    55981 tgaaagggat gaaggtacta aaatatcagg tgatggataa atggcagctt gtgcatttgc
    56041 taagacaatt acggaaccag ctggaatact tgcccttgat ccccaatcac tgaaagaact
    56101 tgaaaaatta ttcgcaccat tgaaactagt acgtaattca taaccgttat ttgtcaaatt
    56161 aattaattcg ttactggcat taaaaatttc aatatattta ttattcgaag tcccttcaac
    56221 atattcacta ataataatag gactgggatc aacataaatc gtcacactgt cttttccaat
    56281 agcgctttca ttatcggtaa caaccagttc aaacattaga gtctctacac tagacggtga
    56341 agtaaaagtg acaatggctt gatcagtagc cgacagtgta actgatgttc ccgaaatttg
    56401 cgtccaagta taactgacaa ttgaaccgtc gggatcatta ctgatcgaac catctaaagt
    56461 cacagttaca ttaaaacgct ccacccggtc taaaccggct cttgccatag gagaaacatt
    56521 accaccgcca ctccagacgt gcgaacctaa ataagtaaat gtattctgag gatgaacttc
    56581 ccattctgaa tcatcggccg aagtaccggc tgaagaggtc caatcggtat tgccctgtga
    56641 tacagtcggt tttcgcacta aagtatgctc agccatcgca tcggtaactc cagcaacact
    56701 ccaagcagta ccgggatctt cgccaaattg tcccaaaaca tcaatagtcg tccatgtccc
    56761 actgataagt ttttgaagcc caagataatc gtctccgtta aaatacgtta tagtatgagt
    56821 tgtatttgaa acagataata ttgcacttgc tgcagatgga ttaccaacta cataaacaga
    56881 tttatcattt aatgtaccag atagaacaat cgaatcttga acaacagtgg ctccattatt
    56941 ggatcggact atacgataat ttgtcaaatc gacaattgtt ccagtactat tgtaaatttc
    57001 aactgcttta ttattactcg atccttcgat atattcgctg aagaacagat caccggccaa
    57061 aatcagggtt ggcaaaatca aagcgatcag gaaaattttg atggtttgat gcataataac
    57121 ctctctaatt ttttaaatac cttttgaacg tt
//
LOCUS       DAOSMP010000012        52710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_675_length_52710_cov_16.494787, whole genome shotgun sequence.
ACCESSION   DAOSMP010000012 DAOSMP010000000
VERSION     DAOSMP010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 52710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52710
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_675_length_52710_cov_16.494787"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1113)
                     /locus_tag="PKX09_03515"
     CDS             complement(31..1113)
                     /locus_tag="PKX09_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOU16922.1"
                     /translation="MNNTSATPTSQLSKTIWRIFLIIMGVAFIFSIFSYVKTVLLMLL
                     IAWLISVILNPLVDYLESIGFNRALAILTIMAIILGGIIALIIGIIPTVIKAVESISA
                     TLKSDLISNLTIQLEQFFQEKFNNPDLARDLINKAEQIGGALLVEVAGFLKNAGTYVA
                     FVGIVPIITFFLIKDRRLFKRALISQVPNRYFELFLNVLHKVDSQVTKYIQGQAIDAL
                     IVGLLSVIGLFIINLVFDNPVPYFFVIGMIAGVANLIPYLGPYVGAVPAIVLTILNNP
                     NNVGYIIFWIIVVFVLVQLIDNNLVSPMVVSKSVNMHPITVVIAVIIGGNIAGMFGML
                     FAVPFWGIIKVTMREVSWGLRSYKLK"
     gene            complement(1106..1630)
                     /locus_tag="PKX09_03520"
     CDS             complement(1106..1630)
                     /locus_tag="PKX09_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOU16923.1"
                     /translation="MNHPLWDPISAKNKSEMSDIMQILAKVPIFSDLDSKELKEVEKI
                     IYHRKYKKGEPIFRMGDPGLGMYIIINGAVEIVEEPENGTRTKLAELEKGAFFGDMAL
                     LDEKPRSASAIAMVDSDIIGFFRPDLVDLLYRKPRMGIKILWALSRVVGERLRKTDEQ
                     LTNLQREMKAKRHE"
     assembly_gap    1723..1822
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1823..2932
                     /locus_tag="PKX09_03525"
     CDS             <1823..2932
                     /locus_tag="PKX09_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOU16924.1"
                     /translation="EYQSEQLEKIRKEIRNYKSDLKKAESKEKSILTQIDETERSISL
                     TEKLIAQLNREQIQKEREIEQIERSIKQLESDLNTLKNDFAKRLVYLYKNGKYNDLEL
                     ILTAKSINQAFYRYKYLRILADIDRRTCTLIRKDINTINQKKQQLNQELRNKEKIINE
                     KKDLMANLQQQKRRHNVQLTKARQNKSNIAAQIKEKEKNAAEISKLIANLDKEREKRN
                     KELERQRTLLGVAEDNPFLASQGELIWPAKGEIVSRFGIQKHPTLKTITDNPGIDIKV
                     KLGTPVVSVLDGVITTITYIRGFGNTIIIDHGSGFYSVYSHVENIRVAENDYVTANSV
                     IAEVGQSGSLSGPMLHFEIWRNKTKLNPEEWLNKR"
     gene            2929..4059
                     /locus_tag="PKX09_03530"
     CDS             2929..4059
                     /locus_tag="PKX09_03530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOU16925.1"
                     /translation="MTLFPLINQTENRNLLRNIVATGRVANTYLFYGPEGSGTEGFAL
                     EFAAMLNCSSKSERPCGICQSCKQMKTLEHPNLTLVFPIPGSPDSKPGDSPFKKFKEG
                     EMDEIRQIIQNKSRNPYDKIIVKKALIIPINFIREIKRHIYLTSQNGGQKVVVIFDAH
                     LMNDEAANAFLKILEEPPDNSTIILSTAYPDQLLPTVQSRCQWLYFPPLPEAELKEFL
                     IKKGYPLENIRLAIRLSGGNVSQLTQLIEHDLPDIKELTLAIFLDIAAWKVSNLYNHI
                     QKMTTIYRQDPEKFAQLMQSLIFWIRDAELIRQGQPIEDIIHLDYAERLTNFVQKFPD
                     FDAQNMKTSVENCVDFINKNVYINLALIEMFFKLKKQLNNSK"
     gene            4062..4883
                     /gene="ricT"
                     /locus_tag="PKX09_03535"
     CDS             4062..4883
                     /gene="ricT"
                     /locus_tag="PKX09_03535"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOU16926.1"
                     /translation="MNTSNKLIKIKFKGGNRQLCQNPLDFPVQPNDKMVVSADRGEEL
                     GCALSSGSPKNLPEELGEIPSLLRKATPQDLSQSSENRLQEKMALEFCVAKISELNLP
                     MKLIDVDVRLDRRKIVFNFTADERIDFRELVKLLAAEFKTRIEMRQISSREEMKRTDG
                     VGLCGLRLCCASFIDDFDPISTQFAKEQNLPMNPAKISGCCGKLKCCYRYEHDFYEEV
                     LKNYPAYGTKVKVADKTGVLEKIDIFAQTLTIRYDDNTAEDFPLTDFNQKITTVQ"
     gene            4897..>5886
                     /locus_tag="PKX09_03540"
     CDS             4897..>5886
                     /locus_tag="PKX09_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOU16927.1"
                     /translation="MNNQEHFFVTTPIYYVNDKPHIGHAYTTILADVLARFQRSNGVE
                     TFFLTGVDEHGQKVQQAAASAGISPQEHCDRMVVYFREAWQRLGIKYDFFIRTTDQFH
                     VRAVQQVLQDLFNKGEIYLHEYGGYYCVGCERFYTPKELVNGQCPQHLKEPDFIKEKN
                     YFFRMSKYQDWLIGYIQDHPNFIRPESRKNEVLGFLKNPLGDLCISRPKSRLSWGIEL
                     PFDQDYVTYVWFDALLNYITAIGYPNDRQRFAKWWPADFHLIGKDIVTTHCVYWPTIL
                     KAMDLPMPKTIFAHGWWMIDETKMSKSLKNIVEPLDLVDAYGVDPVRYFLMRDM"
     assembly_gap    5887..5986
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5987..6625
                     /gene="metG"
                     /locus_tag="PKX09_03545"
     CDS             <5987..6625
                     /gene="metG"
                     /locus_tag="PKX09_03545"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HOU16928.1"
                     /translation="RSINKYLEVRQPWHLIKSDKTAAGTVLFTALEGLRLAATLLEPI
                     MPQKIAELLNALPVDENKPPFEWGRIKSGATLGKLPILFPRIDKIVKPMVEPPSTPPQ
                     PAEPLITIDDFAKIKLTTAKVLTAEKVPDATKLLKLTIDIGTEKRQIIAGIAEYYSPE
                     QIIGKTIVVVTNLQPATIRGIKSEGMLLAAKKDGKLTLLSSLEPIEPGAKIS"
     gene            6630..7388
                     /locus_tag="PKX09_03550"
     CDS             6630..7388
                     /locus_tag="PKX09_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOU16929.1"
                     /translation="MFVDTHAHLTYPDLIVKLDEIIKRARDAGVSRIINIATDLTSSE
                     IVIESAIKYDGVFAAVGIHPQDSANLPADWHQTLRNLLRQKKVVAVGEIGLDYYREYS
                     PKATQQEVFRTQVLIARELGLPLILHDRQAHADLKRILMEIGYYRGVLHCFSGNADFA
                     REMTDLGFYISFTGNATYGNKKTEAAVQAVSLDKLMLETDAPFLAPAGKRGEINEPAN
                     IPLIAEKIAELKNKTLAEIATATTRNAVKFFNLP"
     gene            7388..8182
                     /gene="rsmA"
                     /locus_tag="PKX09_03555"
     CDS             7388..8182
                     /gene="rsmA"
                     /locus_tag="PKX09_03555"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOU16930.1"
                     /translation="MEHYPKKRLGQNFLIDPNILRKIVRVIEPQRDDIIVEIGPGKGA
                     LTRFLLESNAEIHAIELDPELVNYLKQELGQFENFHLYYADAVRFDFSDLFNRNQKIK
                     IAGNIPYNITSPLLFRLFEITDWLDRIVLLVQKEIALRLCAQPATKEYGILSVMTKFY
                     CRPKIEFNVSPQVFRPIPRVTSSVVKLVPIENNYSPEFRRQFNELVRKAFNRRRKILR
                     NSLDLPASTDISACPIDLLRRAETLSVAEFIELTHWYYAHFFLDKK"
     gene            8218..8290
                     /locus_tag="PKX09_03560"
     tRNA            8218..8290
                     /locus_tag="PKX09_03560"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8251..8253,aa:Ile,seq:cat)
     gene            8640..9326
                     /locus_tag="PKX09_03565"
     CDS             8640..9326
                     /locus_tag="PKX09_03565"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: protein motif:HMM:NF009695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HOU16931.1"
                     /translation="MKIHSIKSIIFDLDGTLVDTAGDILAAVNFTMKKFSLKLISYEQ
                     CLRFLGDGVEDLIRRAVANSLREPIDPNYLAEIVAQYKDFYSSHLTDRSRLFPGVRET
                     LAELQDYTLVVVSNKSHDFCVQILRQLDIEKYFQLIIGGDLLPSRKPDPGQLLYVMQK
                     FNLTAAQILIVGDSENDILAAHSIGAPVVVVKYGYRDIEILQEYHPDYMIDNFSDLLK
                     ILKQEIQTFK"
     gene            complement(9311..10432)
                     /gene="alr"
                     /locus_tag="PKX09_03570"
     CDS             complement(9311..10432)
                     /gene="alr"
                     /locus_tag="PKX09_03570"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOU16932.1"
                     /translation="MHFPQNTTIAEVDLNNLRYNYQNIKQKVAPAQIMAVVKANAYGH
                     GAVPIAQCLRDQGVQYFAVARLCEAMELIQSGIGGEILIFGTLYDEEASFAIAHNIRI
                     TVTRAEDFVTYDRLAGALQKVAKVHVKIDTGMGRVGLPAIQAFDLIKASRKLKNIEIE
                     GICTHFATADSRDKTFAYEQLARFNQIIKQLRQTGITSPLIHAANSGAILDLPETYFD
                     MVRPGITLYGHYPSLETSESIPLRQVMSLKTRVAQIREVPGGTSISYGRLYFTKKVTR
                     IAVLNIGYADGMPRAFSNKGQVMIKGQLFPIIGAVTMDQLMVEVGDADVKPGDEVLIW
                     GNSPDGILSATQVAAKAGMLIYELCCAVAPRIPRVYLNV"
     gene            complement(10484..10945)
                     /locus_tag="PKX09_03575"
     CDS             complement(10484..10945)
                     /locus_tag="PKX09_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16933.1"
                     /translation="MIAYLAGAIEYAPDHGKVWRTEMEKFLQEEFGHSCYNPLIEEHK
                     YLTPDESRNFRGYKQTDPLRFQQIVRKLIRGDLRALETKIDYVICNWDKYAVKGGGTY
                     GEVTYAFYHNIPVYLVSEFPVIEISGWIIGCATKIFSSFNELKEYLKITYK"
     gene            complement(10942..11688)
                     /locus_tag="PKX09_03580"
     CDS             complement(10942..11688)
                     /locus_tag="PKX09_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU16934.1"
                     /translation="MIFKKIMLKNELRLDLHKILLGLFFNIIGTCLVYFALVTSLGKI
                     LPRIDYLEVDKFILSAIIGFTSGLMALGLASSYVTHYFQDSRGDYGRLPGMPISQIYS
                     SFLIRLWIILILHLLVTTLTVLILSGFFPGLGRLLLFWIFAILPLGIFIQLGMLIGLA
                     EDRRNQFYWIFLILVPLFLMSGMIIPTHFHTGFISKLLMALPSTVLIEGGRSILTRQP
                     FEFLNILYLIVLNILLFIGGIFFFKRKLQN"
     gene            complement(11691..12614)
                     /locus_tag="PKX09_03585"
     CDS             complement(11691..12614)
                     /locus_tag="PKX09_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU16935.1"
                     /translation="MEASITLRNVSKFFKKKYVLSNLTLGIEKNTFFAIIGRNGAGKS
                     ILLRVISTFMAPDAGEVFINGEDIAKSPQMAKNCIGYLPDHDIHNRWITGWENLRARA
                     ELLEIPQSRFKEICEPRIKDFNLQEVLDEYPVTYSRGAKRCLDLVLVLLGDPQILILD
                     EPTLGLDYQNRTSLFKYLLSVKDHKTIIVASNEFIEVQTLAERWIVLDYGKIRFDGTL
                     EKMLTRVDVPFSGEIILRKKDETLLEKIKESDNFSKVYDLDLTIQLTFERLQDFYAVL
                     ATIPAENLVGVNIQSLSIAEFINRLLSDEEF"
     gene            complement(12614..15331)
                     /gene="polA"
                     /locus_tag="PKX09_03590"
     CDS             complement(12614..15331)
                     /gene="polA"
                     /locus_tag="PKX09_03590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOU16936.1"
                     /translation="MIKIFHIFMEKKETLFLIDGSALAYRSYFGTIRSRLATSRGKPT
                     GAVFAFVNSLKRILEEVKPDYLAIVFDAPEKTFRHQIYTDYKATREAMPEDLVAQLPV
                     MFEIVKAMNIPMLTVPGYEADDVIGTLAKAAKNKGLQVFVVTGDKDFMQLVDDDILVY
                     KPASGQKEVEILDKNAVVSKLGVQPDQITDYLGLVGDPSDNIPGIKGIGPAKAQPLLA
                     KFGSLEKVIEQAESIGNPRLTETIKNGAEIARLSKQLATIKTDVPLDINIESLKIKPV
                     NQAELERIYRELEFYSLIETKPEVSEAVKPDKHYQTIQTQAEVRELVKNLLNKELLSV
                     DLETTSINPMMAEIVGVALCWEAHSGVYLPIRHPAMADQLFAQTDNSAWLAELKPLLD
                     NEQIVKCGQNLKYDMLILRRNGIELRGKIFDTMVAAFLIQPDARSYKLDKLSQYYLNY
                     KMQSIEELIGNGKDQLNMEQVDIDKVTFYAVEDADVVLQLVPIFEEKLKADQLWQVFE
                     EIEMPLVPVLMQLELNGVYLDVKSLKEMSRNLEVQIDAIMRRIYDLAGVEFNINSPKQ
                     LGEILFEKLNLPKVRGNSTDVSVLEKLQHKHPLPSAVLEYRGLVKLKNTYLDTLPLLV
                     NKKTGRVHSSFNQTMAATGRLSSSDPNFQNIPIRTDVGREIRRAFIPQQTGWKILSAD
                     YSQIELRIMAHLSGDAELRRAFQEGVDVHARTAALVYGVPEKDVLPEMRRIAKVVNFG
                     IMYGAGPFRMSEELGISRSDANQLIEQYFRTYPGINDYITKTIAEARQNTYVKTLTGR
                     RRYVPDINSENKNVREAAERVAINMPIQGTAADMIKIAMIRIQRQLTKEKFKSMMILQ
                     IHDELLFEVHPDELERLRQIVTTEMENALPLDVPVKVDIGVGNSWYEAH"
     assembly_gap    15368..15467
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15484..16239)
                     /gene="lgt"
                     /locus_tag="PKX09_03595"
     CDS             complement(15484..16239)
                     /gene="lgt"
                     /locus_tag="PKX09_03595"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOU16937.1"
                     /translation="MYPELLHIGPVTIYTYGLMMAIAFIVCYLIFRAETKKLGDNPDF
                     ASNVVFWAAIGGIVGAKLFYLLENIPDLLADPKGMIFSGSGLVFHGGLIGGTIAVILV
                     LRRSQKSIGVYADIIGPLLLIGQGIGRIGCFFAGCCHGKACDLPWAVTFPNASPPANY
                     PVHPTQLYEAFLNFAFYFILVKVIRPKTTKVGQTMAIYLMFAGLERFLIEFVRVNPRV
                     WRNLTSAQFTSIGLFCAGLIILLFFAKTPKKSV"
     gene            complement(16243..16845)
                     /locus_tag="PKX09_03600"
     CDS             complement(16243..16845)
                     /locus_tag="PKX09_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU16938.1"
                     /translation="MASASDFINIIKTRRTIRQFRQEQVDQAIIIGWVDCARLAPSAR
                     NLQPLEYFLITEPELRARVFPLLRWASYIAPLGDPKEGQHPTGYLVVLINKDLMQSHY
                     AKDAGAAIENFILAAWANGVGTCWLASVEREKLRALLAIPEQYEIDSVVAFGYPAESP
                     VIENRNEIRYWLDEKNTLHVPKRPLASLLHINSWNRHEVE"
     gene            complement(16863..17318)
                     /locus_tag="PKX09_03605"
     CDS             complement(16863..17318)
                     /locus_tag="PKX09_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOU16939.1"
                     /translation="MNIITTLRVSDLTHGLTDAELEALAGLFRCRHIKRDEIIFREGE
                     PSEELYIIAKGRVSVLIYSVSLPGKCEKIATLRDNEIFGEFALIDGSPRSATVVAEED
                     TDLIFVDNVVLYRYLNENEHVGYLIMRNIAKILTGKLRQTDLEMRNAIL"
     gene            complement(17341..18222)
                     /locus_tag="PKX09_03610"
     CDS             complement(17341..18222)
                     /locus_tag="PKX09_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009225787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOU16940.1"
                     /translation="MSNPKLINGVEIAQKVKDDLRPRINRLKQKGITPGLAVVLVGDN
                     PASQSYVNMKAKAFESMQLFSETIRLPGESSQEKILDLINQLNHDSRFHGILVQLPVP
                     KHLDSNAIIQAISADKDADGIHPTNLGKMVLGIEAPLPCTPHGILMLLKYSDIDPTGK
                     HVVITGRSNIVGKPIANLLMQKRPLGNATVTVCHTHTRNLPEITRLADILIAALGQPE
                     FIDRSFVKPGVVVIDVGSNRIEDTSSPKGYRWVGDVKFSEVAEIASAITPVPGGVGPM
                     TIAMLIANTVLLAEKTS"
     gene            complement(18219..19766)
                     /gene="rny"
                     /locus_tag="PKX09_03615"
     CDS             complement(18219..19766)
                     /gene="rny"
                     /locus_tag="PKX09_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOU16941.1"
                     /translation="MTWTYIIFAIGGAVVGTALSLYIYTQKLKRAQATATEIIANAQK
                     EAEEIKREARLEAKDEAIRIRNRAEEEARNKMKDVREAEKKLAKREFYLDRKTELCDK
                     KTEELNKREEELKRHSEIILHKEAELQNIIEAQNERLERIAGMSRKEAEEILMNNLLD
                     KAKESVNKKVKEIRENALAEANRDAKKIIVSAIQRSAADHTAETTVSVVNLPNDNMKG
                     RIIGREGRNIRHFESLTGIEIIIDDTPEAVVLSGFDPIRREVARIALEKLIADGRIHP
                     ARIEEMINKAEKEVDEEILRAAEEAIDEVGISQFPPELLKMLGKLKYRTSYGQNVLKH
                     SIEVAWLSGLMAAELGLNGELAKRAGLLHDIGKAIDRNVTGTHALIGADFVKKFRENP
                     IVINAIASHHEEVEATHPISAIVQAADQISGSRRGARGDTLESYIQRLEKLESIATSF
                     AGVTKTYAIQAGREVRVIVENGVVSDAEADNLAEEIAAKIQQELNYPGQVKVVVVREH
                     RSVSFAK"
     gene            complement(19872..20177)
                     /locus_tag="PKX09_03620"
     CDS             complement(19872..20177)
                     /locus_tag="PKX09_03620"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOU16942.1"
                     /translation="MEEPNVNIVRVTIYGQEYSVKSRADRDYIISVANYVNEKMEEVE
                     STLRTIQPSIRVAILAAMNITDELFTANQEKDKIVSSVEEKAGFLIDIIDEQLKEEI"
     gene            complement(20227..20427)
                     /locus_tag="PKX09_03625"
     CDS             complement(20227..20427)
                     /locus_tag="PKX09_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16943.1"
                     /translation="MNIVNLNLLEEKIKSLLLLVTHLKEENEKLKSELKEGASSEVML
                     DLKKRQELRAKIEGMLELLKDY"
     gene            complement(20438..22825)
                     /gene="pheT"
                     /locus_tag="PKX09_03630"
     CDS             complement(20438..22825)
                     /gene="pheT"
                     /locus_tag="PKX09_03630"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOU16944.1"
                     /translation="MIVNTNWLKKYTDFPFSPAELAERLTAIGLEANLIEDKSTGLEN
                     IIVAEILAVRPHPNADRLTICTVSTGAKQFEVVCGAPNVKQGQKVPLALVETTLPNGM
                     TLKPTKIRGVVSEGMLCAEDELGFSNDHSGIMILEQDAAVGSRLSDYLATDGKSLEID
                     LTPNRPDCASHIGVAREIALMTGNELRKPAINLKEIQQPTSDSIEIEIADPVGCPRYA
                     ARLIRGVKIGPSPDWLVKSLESIGQRSINNVVDAANYVLMETGHPLHTFDYAHLKGQK
                     IVVRRAKPGEKVVTLDGVERELSGDVLLICDSEKPVAIAGIMGLANSEINDDTRDVLL
                     ESAYFNPGVIRKGARFLGMQTEASYRFERGTDYEGLLYALNRLAALIVEVAGGQICRG
                     IVDNYPNPIKARRVVLRYERTNRLIGMNFEPDWIKKVLTGLGCEIINSAESEITVTVP
                     TWRPDLEREVDLIEEIVRVYGMSKVPSAKYIQIVPMETINERYDLIEKLRSFVAGCGY
                     SELYCNSLVSAKAAEFGAESGDKLKLRNPLGQDMAYLRTSLLPGMIAAVKHNFNHYRT
                     DLRLFELGFVQAFAPQAETLAEESLHLGVILTGYLDEPNWAYPRRASNIFFLRGLVEA
                     IAKQFGLQPINFQKAANPYYKHLIQILAGEAKLGYLGELAPGYLAKEWDIQLPVNSME
                     LNVEVILKSLNLTKTYHEPPAFPFTERDISIIVDRTIPVDKVIENIRQNGGNLLREIR
                     FYDLYLGKNIDKKEKSLTFNLLFQANDRTLTDAEVDQIMATIYANLSRELNARLR"
     gene            complement(22830..23846)
                     /gene="pheS"
                     /locus_tag="PKX09_03635"
     CDS             complement(22830..23846)
                     /gene="pheS"
                     /locus_tag="PKX09_03635"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOU16945.1"
                     /translation="MDILNRADEVRGQLQKDLQQVSADPQAVENLRIKYLGKKGLITA
                     LFTDFNSITAEQKRQVGKVLNILKKEAEAQIQALNERLKEQVAQDEYFDYSLPGLAPA
                     VGHLHPITQTLNQVISIFREVGFSVEFGPEVETDYYNFQALNFAENHPARDMQDTFFI
                     DEKRLLRTHTSPVQIRVMLKQKPPIRILAPGRVYRNEAINPRSHCVFHQVEGLYVDEN
                     VTFSELKTTLEMFAKRFFGSEVKTRFRPSFFPFTEPSAEMDVSCFICSGKGCRLCKNT
                     GWLEILGCGMVDPNVFVNVGIDPEKYTGYAFGMGIDRTALLKYGINDIRLYFEGDMRF
                     LEQF"
     gene            complement(23851..24198)
                     /gene="rplT"
                     /locus_tag="PKX09_03640"
     CDS             complement(23851..24198)
                     /gene="rplT"
                     /locus_tag="PKX09_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOU16946.1"
                     /translation="MPRANSSVPHHRKHRKIVGMAKGYRGARSKNYVSAKDAVEKALQ
                     YAYRDRRNKKRDFRSLWIARINAAANQHDISYNKFIAGLKKAGITLDRKALAEIAMHD
                     ADGFAMLVALVKG"
     gene            complement(24209..24403)
                     /gene="rpmI"
                     /locus_tag="PKX09_03645"
     CDS             complement(24209..24403)
                     /gene="rpmI"
                     /locus_tag="PKX09_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOU16947.1"
                     /translation="MPKMKTRRSAAKRFTLTGSGKVKRNKAYHSHILTKKSSKRKRNL
                     RQSEILKSADVKRVKKMLGV"
     gene            complement(24415..24921)
                     /gene="infC"
                     /locus_tag="PKX09_03650"
     CDS             complement(24415..24921)
                     /gene="infC"
                     /locus_tag="PKX09_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOU16948.1"
                     /translation="MEKEVRVNEQIRSQTVRLIDFDGKQIGIVSNKEALRISEDRGLD
                     LVEVSPNTNPPVCKIMDFGKYRYQQQMKERESKKKQHVVKIKEVRFRPHIGEHDFEMK
                     INAARRFLGEGYKVKITLMFRGREMAHQETGKELVQKIIEKLADVGTPEKNPVQEGRT
                     IVTYFTAK"
     gene            complement(24918..26861)
                     /gene="thrS"
                     /locus_tag="PKX09_03655"
     CDS             complement(24918..26861)
                     /gene="thrS"
                     /locus_tag="PKX09_03655"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOU16949.1"
                     /translation="MESNRKITIHLPDNSTMEVSAGVSAGQIAKEISPGLFQNALAAK
                     VNDRLVDLSTIISEDARLEILTYNSPESQEILLHSTSHVMAQAVKQLYPQAKVTIGPA
                     IENRFYYDFDIEPPLTDGDLPAIEARMNEIIAADLPIIRREVSREEAIELFSRMGETY
                     KVEIIREIEPDVPLSIYTQGDFTDLCRGPHIPSTGKIKAFKLLNVAGAYWRGDSRNKM
                     LTRIYGTSFATQKELKKYLNFIEEAKRRDHRKLGKSLELFEINEQVGPGLVLWYPRGA
                     LLLQNIKDYWIKEHLKRGYDLVQTPHIGKAQLWETSGHLDFYRESMFDGMTVEGETYY
                     IKPMNCPFHMQIYKSKTRSYRDLPIRYAELGTVYRYELSGVLHGLMRVRGFTQDDAHI
                     ICTPEQLNDEIQSLVLFSFEFIRSFGFKEMDVYVATRPKEKYVGSPENWAEATEALKA
                     ALSKLDIEYHIDEGGGAFYGPKIDIKIRDALNRSWQCTTIQFDFNEPERFNMDYIASD
                     GNKRRPYMIHRAIMGSLERFAGVLIEHTVGDFPLWIAPVQAVVIPVTNNQDQIATEFY
                     KLLIDNGIRAEINLQNDTIGAKIRDAELMKIPFMFIIGEREATGRQISIRRRKIGDTG
                     VMSWEEAIDLIVKEIQLKKESKS"
     gene            complement(27045..27121)
                     /locus_tag="PKX09_03660"
     tRNA            complement(27045..27121)
                     /locus_tag="PKX09_03660"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27085..27087),aa:Val,seq:gac)
     gene            27840..27977
                     /locus_tag="PKX09_03665"
     CDS             27840..27977
                     /locus_tag="PKX09_03665"
                     /inference="COORDINATES: protein motif:HMM:NF024729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HOU16950.1"
                     /translation="MIAEQIAEYIRFYNEERLHSALFYLTPKEVLEGKMEQRLEERQK
                     N"
     gene            complement(28255..29763)
                     /locus_tag="PKX09_03670"
     CDS             complement(28255..29763)
                     /locus_tag="PKX09_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOU16951.1"
                     /translation="MSINNGNNDLVEYLHSLSGEIDPKQGSVAIILAAGHGKRIKSER
                     SKMLHEIWGVPTVVRVANAASKGLASNNQIIVVGIKACEVAESAGKRANRIFVYQTEQ
                     KGTGHAVKEALSAISQNGHIKDIYIFPGDMGLITPAVVSELKRRFEQSGCGLALLTGN
                     YKGEPEANSYGRIIRVPARTATGEPSGEDEGRVIEIKEHKDILAIPESGFYTTVYNGK
                     KYQFKRSELLGITEYNTGVYAMKFDLAQRFIHQLTPDNVQGELYLTDIIGLFNANKIE
                     VEAYPVDDESAVIGFNVKSVLQEMENIARRKVWKLLRDIIYIEDENDFFIADEVVIRI
                     LEMDKINPPLDIEIGKGVHISSGVHLERGVKIRAGSLLNGNIHLGVGTVIQENVSLST
                     YPNQQMTIGKNCVIMRGDLIKGNITIGDNTSIESSVIITGSSEYPTIIGKNVVIKGTT
                     YIFGSIIEDDLFIEHSVLKCKRVERTVRKDGTIQPVRWVMPQPQGLDIVRDL"
     gene            complement(29811..30614)
                     /locus_tag="PKX09_03675"
     CDS             complement(29811..30614)
                     /locus_tag="PKX09_03675"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOU16952.1"
                     /translation="MNNFASRMLINIIGIPAILLLIHLGGIPFVLFITVMVFIAQVEL
                     YLLMRKKGIAPLLLPGVLAGVIWQLASFYLPGALLLYLLIGLVVLLLLASLVRQIKNV
                     TVDLASTLFGFVYLPVLTSTIILMRNIPGRGRSIVLLLFVTIWVCDSLAFIIGKWLGR
                     RKIAPKISPNKTYAGCIGGLFGAVLTVLIFYFLGWAPDFMSIMQLIIFGILAGVFGQA
                     GDFVESAFKRDAEVKDSSNLLLGHGGVLDRFDSFFIAAPVIFLYIITIL"
     gene            complement(30630..31058)
                     /locus_tag="PKX09_03680"
     CDS             complement(30630..31058)
                     /locus_tag="PKX09_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOU16953.1"
                     /translation="MLGFMGCASAPRYLYTSPKTGATNDDKNTLLLLKNSNRTAVLTA
                     SYYGADFHGKLTSSGEVFDMNGMTAAHKSLPFGTVLQVTYPPTGQSVTVVVNDRGPFV
                     PGRDLDLSYAAARKIGLVNDGTGKVKVKVLKWGNNDSPKQ"
     gene            complement(31088..32002)
                     /locus_tag="PKX09_03685"
     CDS             complement(31088..32002)
                     /locus_tag="PKX09_03685"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOU16954.1"
                     /translation="MTDLHIKLREDFILRNPLLTASGTYGYGDEIADLINPELYGGIV
                     TKSISLEPRVGNPPPRIAEVTSGMLNSIGLANIGVDKFLEEKEEFLAAYNGKVIVNIA
                     GRKDDEYVQVVKKLSDRQWVAAFEINVSCPNVSEGGIEFGRDPLILGQLVKKLRHATD
                     KFLIIKLSPNVTDIRVLALAAQENGADALSMINTVYGAAIDIHTRRPKINTVVGGFSG
                     PAIKPIALAHIIKTYKEVDIPIIGIGGISCGADVIEFLLAGAHAVQIGTANFFNPKVC
                     GEMLDFIEQYCTENKISCLRDIIGRVEV"
     gene            complement(31999..32766)
                     /locus_tag="PKX09_03690"
     CDS             complement(31999..32766)
                     /locus_tag="PKX09_03690"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOU16955.1"
                     /translation="MRVLKDTTVLSNRMVATDTYLMEFAFPEISQSAQPGQFVNFYFP
                     ERPQLFPRPFSIAGVNRENLTILYKKVGCLTSIMSQWQPSQKIRVLGPLGNTFNIKYN
                     NHVLIAGGVGLAPLLFLNQKLANLNYKIWNFVGVRSKDELPPLSLLSGEKYIATEDGS
                     VEYCGTVVDCLRNNLGAIPHPFSVYACGPEAMLSALWHLSRENHFDLQLSLEKLMACG
                     LGICQGCAIPHSHPSEQPLRLVCQDGPVFNANELDLG"
     gene            complement(32763..34622)
                     /locus_tag="PKX09_03695"
     CDS             complement(32763..34622)
                     /locus_tag="PKX09_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU16956.1"
                     /translation="MKNNITVKSIETLFLIGLILVLSNCAYYNTYYNAEKYFREAEKE
                     FKTLPDEKISPALKKKFDTAIEKANKVITQYPESRWVDDSYYIIAMSHYYKGDYRIAR
                     QKFEDFAVKFPQSKLYPEAMVWYGRNLWKLDERELAFFQWKKIMNLTDDYYLLAELYL
                     SIGELYFKDSAYDSALFYYQKATEVGGSFDIAAEAQFRIAEIDLALNRPQEAIKNLKK
                     IDQFSPSLILRDKMQVLLVRIYRESGQYDKAIDLINEKLNDPANEKIWGDLELQLGLI
                     YLAQQDYDAAISRLTKVIEDYKGKPVAAEASYHLAELYMTQIHDYTKAAVQYDNVVKI
                     EVNTLRAFECRNKSSEIKRFNTIYNRLAALSEQVAAINLNPPTESDTLGESLPVDQEQ
                     SELKKALEKKNTTQKKTIDTLAVFKEYYTTLYEIAEIFYFSFNQPDSAIYYFEKVAYE
                     IPFNSLRDKALYSLYRIASDRQNIEKANEYANQIKQWFPESIYLAEIEKRPLNTSARE
                     VEADSLLVKAENLDQTDLHSAITIYEKLAKEYTDCQAAEKAVMYLAYFYHHRLFDLNN
                     ALTWYKYYLDTYPNGQYYQIMANAYDQLKEIEDALAQGSEAEENEPQKPEKVE"
     gene            complement(34649..35416)
                     /locus_tag="PKX09_03700"
     CDS             complement(34649..35416)
                     /locus_tag="PKX09_03700"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOU16957.1"
                     /translation="MNLITNEVARKRISQGEQVVVLTGAGISAPSGIPTFRGENGLWT
                     KLSPQELASFESFYLNTAMVSEWYKHRREIIEVCQPNAGHYALVELAKMVSKFTLVTQ
                     NIDGLHQRAGSRDVLELHGTIMENYCIRCGQQYSATEFDEIYNSSFDHIPRCYCGGLI
                     RPNVVWFGESLPKQAYEEAYRASQQADLFLSVGTSAQVRPAADLPLIAKENGAFLVEI
                     NIVETVLTPFTDLFLKGAADEILPQFNREYQYLLMQV"
     gene            complement(35475..38036)
                     /gene="glgP"
                     /locus_tag="PKX09_03705"
     CDS             complement(35475..38036)
                     /gene="glgP"
                     /locus_tag="PKX09_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOU16958.1"
                     /translation="MKPIQSFTVKPILPKKLEELRDLAYNLWWTWNADARDLFRRLDM
                     DLWEETRHNPVALLNRVEQDVLETRAQDDSYLYQLRRVYDKFKQSLNSPTWFEKSGKY
                     NSHLNIAYFSMEYGITECLADYSGGLGVLAGDHLKSASELGIPLQAVGLSYQYGYFQQ
                     VLNVDDYQEEEYPVNDFYNSPLTLVKGSDGKQLLIKVPFPGRLVYARIWKAQVGRVPL
                     YLLDTNIAENSEADKKITAELYGGNTETRIQQEIILGMGGFQALQILDATPFICHMNE
                     GHSAFSGLERIRNVVKNNGLTFYEALEIIKAASVFTTHTPVQAGIDLFHPDLIKRYFD
                     EYCREVGIEINELLALGRNDPDDNSEHFSMAILAINLSSKINAVSKLHQQVAQEMWES
                     LWPDILTTEIPIDYVTNGIHQASWISQEMSDLFDRYLGPDWRREPASKTIWNKVDKIP
                     DGELWRTHERRRERLVAYARSELYRQYVTLGKPQNIIARVKSILSTDALTIGFARRFA
                     AYKRAYLLFTDPERLAKILTNKNFPVQIIIAGKAHPQDEEGKQVIRRILKLSQEEPFY
                     QCVVFLENYDMNMARYLVQGCDLWLNTPRRGLEACGTSGMKAVANGGLHFSTIDGWWD
                     EVYTPGIGWAIGTRDIYEDIDYWDRQEANILYNVLENEIIPSFFERDNDDLPRDWIAM
                     IKASMKAICPQYNSNRMVCEYLDQIYYPAAVRANELSKDNYAVAKELAHWKEKMRKNW
                     DLVRFLKVTSGSIEELSVNSQLEIRAEIYLDGLQVDDVDVQVYYGRVDPNGDIIDGKF
                     SSMKFETKVDSKKYVYVDKINNWESGLNGYTIRIIPKHPALAYPFEDGLIHWFDE"
     gene            complement(38142..39401)
                     /gene="murA"
                     /locus_tag="PKX09_03710"
     CDS             complement(38142..39401)
                     /gene="murA"
                     /locus_tag="PKX09_03710"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOU16959.1"
                     /translation="MSRFIIEGNRNLSGEIKVAGNKNAALPIIAATVLTDEPCILENL
                     PDISDVRSMLAILSDLGKSVEMLSAHKCQITGSVNKSRLNDKLASTLRASILYLSVLL
                     ARTGEVEMVPPGGCVIGRRKVDQHFDALQALGVKVNIIEHGYRLILKRPKPNYVFLRE
                     ASVTATENAMMLAAMTEGKTTLDNAAGEPHVQDLAKVLVKMGAKIQGAGTNRLTITGT
                     KKLKGFRHRIMPDHIEAGTFAIAAACTGGEVVIHDANRENLTIINLILDQMRVKLSFL
                     DEKTLVIKPSKNIRAIPKVQVGVWPGFPTDLMSPMVVLATQARGTTLCHDWMYESRMF
                     FVDKLVTMGAQIVQCDPHRVLVSGPTRLKGQEIVSPDIRAGIALVIAALIARGKSKIE
                     KAELIDRGYEDIVPRFRQLGAEINREQ"
     gene            complement(39419..40471)
                     /locus_tag="PKX09_03715"
     CDS             complement(39419..40471)
                     /locus_tag="PKX09_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOU16960.1"
                     /translation="MFANQDKSSIQKSEEFVSELSRNHITAQKEVPLAKFTTFGIGGN
                     ARLLVIVENTEKLEAVIKLATAFQVPFRIIGCGSNLLVSDDGYDGLIIINRSKSWQII
                     DDCPANLKFSRIIPRYDPLTASQEEEIQAENLPQKIVRVDSGFYNNTLIQEMFEQGLT
                     GLEWFSGIPSSVGGAVYMNMHGAYTYFGDLVYRAKLIDGISPKIVDNAYFQFDYDFSV
                     LQKTREVVLWVELILTMGNVEQARIVAQEWKSRKSFQPRRSAGCIFKNLSPEAQKQFN
                     LPTSSVGYLIDKVLNLKGLRIGGAVISPKHAAFIENDGTARADDVYKLIEIIKAKAKE
                     TLGLELETEVELLGKF"
     gene            complement(40555..43128)
                     /locus_tag="PKX09_03720"
     CDS             complement(40555..43128)
                     /locus_tag="PKX09_03720"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOU16961.1"
                     /translation="MSIVNNSLYCIPIYNPYYSLPFPSQAEINELIDNLLNSLKEGTQ
                     KGVYHFDQMIYPLCEYLKSHPEKTDLVRQLVEEKKIYFGPFFIQLHSGHISGELLIRN
                     LLIGHQEGDKIGDILKYFHLPAMRGRNSQTPQILAGFNIDAVLIDYDIKLMDKNTTEF
                     IWEGLDGTQLLVSRVVFLNPNRLNLAKSKIQETFREYFSHNPNRVLVYLFNDKKSWET
                     MPTYIEKLASIFSSDLKIESLADCIWEIKEQIDSREISEYKGEMCLEKTEDCTETQFD
                     YYLRHSVLIGLSNHRLENLIQYFVEPWSLIMKYLQLKIRPKATNHIWEEFLKNQTFTY
                     LVDTNPNKFIQSLSTTQTGLMNQLEEQVKDLITAVVNNIHIDDYNPNNIYFTVFNPLP
                     YNRSEIVEFVAELSSLINRDTIIAKEVGGRDIPFRVLYKETETLFEYDKADKAKYRCL
                     VELRDLPGMGWKTFEIDPKGKPKLYSAVPISAERNSLENDFLRVEINQNGTLEIFAKE
                     TGEFFSEVAYFQDEIDLGRFSVEDKTATHIPLTTKKLHPQIKLLYNSPKAAAYKIEYF
                     WEIPEMFNWKNSRRSPRHETIKISEVVTLSSLSRMIDLKIEINDHAYDHMIKICFPIE
                     FVPENSYTDGFFSVEMRPFNSPQNLHCQALSMGNFVGVSNESGGFAIFSDGINEYQLL
                     KGKHNVLTLTLVRDFPIVRSKKTGGPILSRNEKTEVHLSIYPRLSDWTSSDILTEAII
                     FNNPIIIRQMDQTNGYLPAKMQFLKVTPDTLFYSALKPTEDEKDVVLRLYNPTRNIIV
                     GEIATYIPIKAARYITVEEHIIAPLEISDPYRLKVKVFPQKIMTLKIIFDRLPINLSG
                     S"
     gene            43332..43817
                     /locus_tag="PKX09_03725"
     CDS             43332..43817
                     /locus_tag="PKX09_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16962.1"
                     /translation="MNLKKKISIGGMIELRDLMMVSIFSFPDQPGSAGKVLTYFGQRG
                     ISVDFITESRNLEGSADLIFCINQKYKSDITQTFQELSAIIPIKDTKITEPVAIVSFY
                     GPHFREKPAIAGTICQTLGEAGINILGISTSISSVSCVINEHQVDAARQAITANFNLP
                     E"
     gene            43820..45004
                     /locus_tag="PKX09_03730"
     CDS             43820..45004
                     /locus_tag="PKX09_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOU16963.1"
                     /translation="MNLPDSFTSDESKTREQLLWELQQLRNRINQLEAADLERRRIEK
                     SLNKYEKNFRELVNLLPQTIYEMDKNGKFRFTNQVGFETFGYNQQDIDTGVNFSQLFI
                     PEEREKLNRNIQKIYNGSDLEGHEYTALRKDGTTFQALIYSSPIFYDNKPVGLRGVVI
                     DITDRKKVEQDLKDSRDQFRNLSAHLQSVREEERSYIAREIHDELGQALTALKMDLIW
                     INKRLTTAQSEIAEKISTMFDLIDKTIQTIRRISTDLRPGLLDDLGLTAALEWYCEDF
                     QNRTGIHCNLHLRPAEITIDQERSIAIFRILQETLTNVARHAHASEVKVNLGIKDHAL
                     EMKIVDNGIGITDEQVNDPQNLGLVGLRERVYPWNGHIAIKGRPQKGTTVLVFIPLDG
                     NI"
     gene            45004..45636
                     /locus_tag="PKX09_03735"
     CDS             45004..45636
                     /locus_tag="PKX09_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU16964.1"
                     /translation="MINLLIVDDHPLIRQGLRQTFLDTNDIRVSGEASDSTYALAEIE
                     NHNYDAVILDLSLPGRSGLDLLKQIKNLSPSLPVLILSVFPESQYAIRVLKAGADGYI
                     SKESHPDELIQAVRKIVTGGKYISPTLAEKLANSLTETTQNPTHEILSDREFQTMLAI
                     ASGKTVKEIAAELNLSVKTISTYRTRILRKMHLKNNAEIICYAVRLGLVQ"
     gene            45857..46462
                     /gene="lexA"
                     /locus_tag="PKX09_03740"
     CDS             45857..46462
                     /gene="lexA"
                     /locus_tag="PKX09_03740"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOU16965.1"
                     /translation="MAEKLSPKKQRFVNFVHDFTETNKRAPTFLEIMHALQIKSPGTV
                     SWYVRELEKAGVLRRSGPPNAKRALSVSETESASTLPLLGLIAAGYPLEAIENQETIA
                     VPPAYIHPSNYVLKVKGDSMIDDNIQDGDYIIVRQQAQAESGQTVVAFINNEATLKRY
                     YPKAGGVELHPRNPAYPVIKVKPGDEFRLGGVVLAVWRVYE"
     gene            46561..46959
                     /locus_tag="PKX09_03745"
     CDS             46561..46959
                     /locus_tag="PKX09_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOU16966.1"
                     /translation="MSTKQVPLSPREEEIAKKIVSAAFNVHQHLGPGLLEKIYETCFC
                     YELSKYGLHYRQQVEIPITYDNIVFDAGLRLDILVEDLVICELKAVTEMNPVFQAQIL
                     SYLKIAEKRLGFLINFNVPLIRDGIQRIIL"
     assembly_gap    47053..47152
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            47201..48454
                     /locus_tag="PKX09_03750"
     CDS             47201..48454
                     /locus_tag="PKX09_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16967.1"
                     /translation="MSESVLHIRLSDFELQVERMLDSGLRTRAIAVISSHHPNGTIVC
                     ASPEALSEGIYPGLKVTIARKMSHSVALLPYNSTLYTKINRYVYQTITRYSPVIEPTV
                     YGQYFADLSGMHGIYRDSLQAGNLIARDIQSKVSLQSHIGISVNKLVSQITTAVVPET
                     IHRVEPGTEPNFLAPLNTEVLPVTNEPPVNKLVRFLFLSQVQNVQQVTTESETGAILF
                     GKYYRRLFREARGQDNSPVRPPQKTDRITEQTVLTADTNDEVQLLAAVHNLAEQIAYQ
                     LRRRRQIVRTLNLEIHYTDGFKSNRKGAVIANDDTTVIAEASRLFSLANYRRNRVRAI
                     ILDATDFQPVINQLDLFRPPKDDRLSQALDQIRQKYGFASIGSADNLLIPLNKTTKAL
                     RHKDIFGAVISNKNVSCSDKIALPA"
     gene            48720..51746
                     /locus_tag="PKX09_03755"
     CDS             48720..51746
                     /locus_tag="PKX09_03755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOU16968.1"
                     /translation="MFVHLNTHSTYSKMRGTASPQALLTQAKAAHQTHLALTEVNGLW
                     GFIRFVQWAREVGIQPIAATNLLTPNFDLILLVENQYGYENLCRAISRVHDNENLSIV
                     ELLKPYAKGLFVLAYQEDVLSELACFIPATHLFVELRPGVSEQETRRLAETFHLEIVA
                     TGDVYFLKPEDQKTHRILRAIDKNSTLAQLAPSDYKSEQHFFRTEKEMIDLFPNSLNA
                     INNSQFLAERCKTDWNYTNTIFPGLSLKETNLANRQLRQLVYDGAKIRYGKIDQVVKK
                     RIEYELSLITQKGYAPYFLIVYDIVQQTPTTIGRGSGAASIISYCLFITQVDPIKYNL
                     CFERFIHPERIDMPDIDIDFAWDERDAILDYIFKKYGLERTAMVSNQVMLQPRSAIRE
                     VAKVYGLSNEEIKAVTKRVGYFARKTELLEWLSTDPRFRNLDLDDTLREIILQSEKIV
                     GVFQVSSVHPGGVIIVPDEIRQYAPVLTATKGVQIVEWEKDQVEDSGLLKIDILGNRS
                     LAVVRDTLKQIGLYRSEFMDYHKIQPVGDSATQDLMQSGRTMGIFYIESPATRLLLKK
                     AGRVDFEHVVIYSSIIRPAANRFINIMLARIHGQPWQLLHPDLKCLEESYGIMVYEEQ
                     VSMVAREIAGFSYAESDYIRKVISRPALAHLVPIWKKKFINGALARGYSPKLAETLWE
                     MIESFSGYSFCKPHSASYAMLSFTCAYLKAHFPAEFLAAVISNQGGYYSTYAYLSEAR
                     RWGIQILPPHINRSWQEYRGRRNKIRMGFMAIRNLQDKAVEAILKERKNGDFKNLDDF
                     LKRVDLDLADAMALTNAGCFAEFEPQLSHREIAFKVASYYLQKGDRRPLPPPIDRQPI
                     SRLEKLTLEIESFGFPIAEHPLERYLPVFAGKVKKARDLAQYVGQTINLLGVYITRKV
                     ALTKQHDPLEFVTFEDETDIFECVMFPESYKEFGDLLNWETLFVIRGKVEEAYQTYTV
                     TIEKLASLQRTVEKMSSGRRMLVKTSSAETHFAKEY"
     gene            complement(51855..52289)
                     /locus_tag="PKX09_03760"
     CDS             complement(51855..52289)
                     /locus_tag="PKX09_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16969.1"
                     /translation="MMWNEFGELAQNEWLKTGIIRPNIVIDAFVVMPNHLHGILIIND
                     NDDDGRGFRGGGNRRDTLQRVSTTITITITITDTNTITDTDTITDTEMGTETDTGTIE
                     QFGKPTKNSIPTIARLFKSTTTRQYIINNPKQWKEGNLNKFL"
BASE COUNT        15153 a        11879 c        10294 g        14984 t
ORIGIN      
        1 aagatttacg ttgatttata gattgctgag ttatttgagt ttgtaacttc ttaaacccca
       61 gcttacttct cgcattgtaa ctttaataat tccccaaaac ggaaccgcga acagcatccc
      121 gaacatacct gcaatattac caccaattat caccgcgatc acgacagtta tcggatgcat
      181 attgacgctc ttagagacta ccatcggtga gaccagatta ttatcaatga gttgcacgag
      241 aacaaatacc acgataatcc aaaaaatgat atatccgacg ttattcggat tattcagaat
      301 agtaagtaca atagcgggaa cagcaccgac atatggcccg agataaggaa taagattagc
      361 gacgccagcg atcattccga taacaaaaaa atacggcacc ggattatcaa ataccaaatt
      421 gataatgaat aagccaatta ccgacaataa gccgacgatc aaagcgtcta tcgcttgccc
      481 ctgaatatat ttcgtaacct gagaatcaac tttgtgcaag acatttaaaa ataattcgaa
      541 ataacgattt ggaacctgac tgattagtgc tctcttaaat aatcgtcgat ccttgatcaa
      601 aaaaaaggtt ataattggta caattccaac gaatgctaca taagtgccgg cattttttaa
      661 aaaacccgcg acttccacca gtagcgcacc cccgatttgt tctgccttat ttatcagatc
      721 gcgcgccaga tcgggattgt tgaatttttc ctgaaaaaat tgctcaagtt gaatggtcaa
      781 gttactaatt aagtcacttt ttaaagtagc cgagatggat tcaaccgctt ttattacggt
      841 cggaataatg ccgataatta aagcaattat tccaccaaga ataatagcca taatggttag
      901 aattgctaag gctcggttaa atccaatact ttcaagataa tctactaatg gattgagaat
      961 caccgaaata agccaggcaa ttaacaacat taataacaca gttttaacat aggagaagat
     1021 cgaaaatata aatgccactc ccataataat taaaaatata cgccagatgg ttttacttaa
     1081 ctggcttgtt ggagtagccg atgtattatt catggcgttt cgccttcatt tcacgttgta
     1141 gattagtcaa ttgttcatct gtttttcgta aacgctcacc aactactcgg gataaagccc
     1201 agaggatttt gattcccata cgaggtttgc gatacaataa atccactaaa tccggtcgga
     1261 aaaaaccaat tatgtcagaa tcaaccatcg cgattgccga ggctgaacgt ggtttctcat
     1321 cgagtagagc catatcccca aaaaaagcgc ctttttcaag ttccgctaat ttcgtccggg
     1381 ttccattttc tggttcttcg acaatttcca ccgctccatt aatgataata tacattccaa
     1441 gccccgggtc acccattcgg aatatcggct cgcctttttt atatttacga tggtaaatta
     1501 ttttttccac ctcttttaac tccttagaat ccagatcaga gaagataggc actttagcca
     1561 gaatttgcat tatatcgctc atttccgatt tatttttggc tgatattggg tcccataatg
     1621 gatgattcat tttatctatt ccttttactt cattttccaa taatatcgag gtaatataca
     1681 aacaatttat ttatacaaac aattccaata agtcaagact tcnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nngagtatca atcggagcaa ctggagaaaa tccgtaaaga
     1861 aatccgcaat tataaaagtg atcttaaaaa agccgagtct aaagagaaat caattttgac
     1921 tcaaattgat gaaaccgaac gcagtatcag cctgaccgag aaactcattg cccagcttaa
     1981 tcgcgaacag attcaaaaag agcgcgaaat cgagcaaata gagcgttcaa ttaagcaatt
     2041 ggagtccgat ttaaatactc ttaaaaacga ttttgccaag cgccttgttt atctttataa
     2101 aaacggtaaa tacaacgatc tggaattgat cttaactgct aaatccatca atcaggcttt
     2161 ttatcgctat aaatatctgc gcattctggc tgatattgac cgccgtactt gtactcttat
     2221 tcggaaagat atcaatacta taaatcaaaa aaaacaacaa ctcaatcaag agctaagaaa
     2281 taaagaaaaa atcatcaatg aaaaaaaaga cctgatggct aatcttcaac agcaaaagcg
     2341 tcgccataat gtccagctca caaaagcccg tcagaataaa tcaaatatcg ccgcccaaat
     2401 taaagaaaaa gaaaaaaacg ccgccgaaat ttccaaattg attgctaacc tggacaagga
     2461 gcgcgaaaaa cgcaacaaag agcttgaacg tcagcgcact ctattgggcg tggcggaaga
     2521 caatccattt ctcgccagtc agggcgaact aatctggcca gctaaaggtg aaatcgtctc
     2581 gcgctttggc attcaaaagc atcctacgct aaaaacaatc accgataatc ccggtattga
     2641 cataaaagta aaactcggaa caccagtggt ttccgtcctg gacggagtta ttacaactat
     2701 tacttatatt cgcggctttg ggaatacaat catcattgat cacggctccg gtttttattc
     2761 ggtttattcc catgtcgaaa atattcgcgt cgctgaaaat gattatgtta cagccaattc
     2821 agtcatcgcc gaggttggtc agtccggctc gctgagcggc ccaatgttgc attttgaaat
     2881 ctggcgtaat aaaacaaaac tcaatcccga agaatggctg aataaaagat gacactattc
     2941 ccccttatta atcagaccga aaaccgaaat ctattgcgca acatcgtcgc cactggcaga
     3001 gtagcaaaca cctatctatt ttatggcccg gaaggtagcg gaacagaagg cttcgccctt
     3061 gaatttgcgg caatgctgaa ctgctcctca aaaagtgaac gaccatgtgg tatctgtcaa
     3121 tcttgtaaac agatgaaaac cctcgaacat cctaacctaa ccctggtttt cccgattccg
     3181 ggttcaccag actctaaacc aggcgattcg cctttcaaaa agtttaaaga aggggaaatg
     3241 gatgaaatcc ggcaaattat tcagaataaa agtcgtaatc cttacgataa aattatagtc
     3301 aaaaaggcgc tcatcattcc catcaatttt atccgtgaaa ttaaacgcca tatctatctc
     3361 acttctcaga atggcggtca gaaagtcgta gtcatttttg atgcgcattt aatgaacgat
     3421 gaagccgcta acgctttcct gaaaatcctc gaagaaccac ccgacaattc tactataatc
     3481 ctctccaccg cttatcccga tcagctcctg ccgaccgttc aatcacgctg tcagtggcta
     3541 tacttcccgc ctttgccaga agctgaactg aaagaatttc tcataaaaaa agggtatcca
     3601 cttgaaaata tccggttggc tattcgtctc tcaggcggca atgtcagcca gcttactcaa
     3661 ctgattgaac acgatttacc agatattaaa gaattgaccc tggcaatttt cctggatatc
     3721 gccgcctgga aagtttccaa tttatataat catattcaaa aaatgacgac tatctatcgc
     3781 caagaccctg aaaaattcgc ccaactgatg caatccctga ttttctggat tcgcgatgcc
     3841 gaattgattc gccagggaca gccaattgag gatattatcc atctcgatta cgccgaaaga
     3901 ctaactaatt tcgttcaaaa atttccggat ttcgacgccc aaaatatgaa gacttccgtc
     3961 gagaattgtg ttgatttcat taataagaat gtctatatta acctcgcctt aattgagatg
     4021 ttctttaaac tgaaaaagca acttaataat tctaaataac aatgaatacc agcaacaaac
     4081 ttatcaaaat aaaattcaaa ggcggtaatc gtcagttatg tcagaatccg ctcgactttc
     4141 cagttcagcc gaatgacaaa atggtcgtat ccgccgatcg gggcgaagaa ctcggttgtg
     4201 ccctatcatc gggctcacct aaaaatttac cggaagaatt gggtgaaatt ccctctctct
     4261 tgcgtaaagc caccccgcaa gacctatcgc agagtagcga aaatcgcttg caagaaaaaa
     4321 tggctctgga attctgcgtc gctaaaatta gcgaattaaa tctgccgatg aaattaattg
     4381 acgttgatgt gcgtcttgac cgtcgtaaaa ttgtatttaa tttcacggca gatgaacgaa
     4441 ttgatttccg cgaattagta aaactcttag cggctgaatt taaaactcgc atcgaaatgc
     4501 gccagattag cagccgcgaa gaaatgaagc gtaccgacgg agtcgggctc tgcggtttga
     4561 gattatgctg tgccagtttc attgatgatt tcgatccaat cagtactcaa ttcgctaaag
     4621 aacaaaattt gccaatgaat cccgccaaaa tctccggctg ttgcggcaaa ttgaagtgct
     4681 gttaccgcta cgaacatgac ttttacgaag aagtattaaa aaattatcct gcttatggca
     4741 ccaaggtcaa agtcgccgat aaaaccggcg ttctcgaaaa aattgatatt ttcgcccaaa
     4801 ctctcactat tcgctacgac gacaatactg ccgaagactt tccattaacc gatttcaatc
     4861 aaaaaataac tacagtccaa taattttcac caaactatga ataatcaaga acatttcttt
     4921 gttacgactc ccatctatta tgtcaacgac aaaccgcata ttggtcacgc ttatacgacc
     4981 attctcgctg atgttttggc gcgttttcag cggagtaacg gtgtcgaaac tttcttccta
     5041 accggtgtgg atgaacacgg ccagaaagtt caacaagccg ccgcctctgc cggtatttct
     5101 cctcaggaac attgtgaccg aatggttgtc tatttccgcg aagcctggca aagattagga
     5161 atcaaatacg acttttttat ccgcacgacc gaccaatttc atgtccgtgc tgttcagcaa
     5221 gttctacaag atttgttcaa taagggtgaa atctatctgc acgaatatgg cggctattac
     5281 tgcgtcggat gcgaacgatt ttatactcct aaagaactgg ttaatggtca atgtccccag
     5341 cacttgaaag agccggattt catcaaagaa aagaattatt tcttccggat gagtaaatac
     5401 caggactggc taatcggtta cattcaagac catccgaatt ttattcgacc ggaaagccgc
     5461 aaaaacgaag tactgggctt tttgaaaaat ccgctgggcg atttgtgtat ttcccgtccc
     5521 aaatcccgcc taagctgggg tattgaactg ccgttcgatc aggattatgt cacctatgtc
     5581 tggttcgatg ctttgctgaa ttatattacg gcgattggct atccgaatga ccggcaacgt
     5641 ttcgcaaaat ggtggcctgc tgattttcat ctgattggca aagacatcgt tacaactcac
     5701 tgcgtatatt ggccgactat attgaaagct atggatttac cgatgccgaa aaccatcttc
     5761 gctcacggct ggtggatgat tgacgaaacc aaaatgtcaa aatcactaaa aaatattgtc
     5821 gagccgctcg atctggttga cgcttatggc gttgacccgg tgcgctattt cctgatgcgc
     5881 gatatgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgat ctataaacaa
     6001 atatctggaa gtgcgccagc cctggcacct cattaaaagc gataaaaccg ccgccggaac
     6061 cgtgcttttt accgcgctgg aaggcttgcg tctcgccgcg acactactcg aaccgattat
     6121 gccgcaaaaa attgcggaat tgttgaacgc tctgccggtt gatgaaaaca aaccgccttt
     6181 cgaatgggga cgaatcaaaa gcggtgcgac tctcggcaag ctcccaatac tcttccctcg
     6241 aattgataaa attgtcaaac cgatggtcga accgccttcc accccacccc aaccggctga
     6301 accattaatc acaattgatg attttgccaa aattaaacta acaaccgcta aggttctaac
     6361 tgctgaaaaa gtgccagatg ccactaaatt actaaaattg actattgaca tcggcacaga
     6421 aaaacgtcaa atcatcgctg gaatcgccga gtattattca ccggagcaaa ttatcgggaa
     6481 aacgattgtc gtcgtcacta atctccaacc ggcaacgatt cgcggtataa aaagcgaagg
     6541 tatgttatta gccgccaaaa aagacggcaa actgacttta ctcagtagtc tcgagccgat
     6601 cgaaccgggc gcaaaaatca gctgattaaa tgttcgttga tacacacgcc catctcacct
     6661 accctgatct gattgttaaa ctcgatgaaa ttattaagcg cgctcgcgat gccggagtca
     6721 gtcgcattat taacattgct accgacctaa cttcttctga aattgttatc gaatcggcaa
     6781 taaaatacga tggcgttttt gccgcagtcg gcattcatcc acaagattcg gccaatttac
     6841 cggctgactg gcaccaaact ttgagaaact tactccgtca aaaaaaggtc gtagccgtcg
     6901 gcgaaatcgg gctcgattat tatcgcgaat actctccaaa agccactcag caagaggttt
     6961 ttcgtactca ggtccttatt gctcgcgaat tgggtctgcc gttgattctg catgatcgcc
     7021 aggctcatgc cgatttgaag cggattttaa tggaaatagg ttactatcgc ggcgttttac
     7081 attgcttctc tggaaatgcc gactttgccc gggaaatgac cgaccttggg ttttatattt
     7141 ctttcaccgg caatgccact tacggcaata aaaaaaccga agccgccgtg caggcagttt
     7201 ctctcgacaa actaatgctc gaaaccgatg cgccgtttct ggcgccagcc ggcaaacgcg
     7261 gtgaaatcaa cgaaccggct aatattcctc tgattgccga gaaaatcgcc gaattgaaaa
     7321 ataaaacgct ggctgaaatt gcgactgcca ctacccgaaa cgcggtgaaa ttcttcaatt
     7381 taccgtgatg gaacactatc ctaaaaaaag acttgggcaa aattttctga ttgatcccaa
     7441 tattctgcgc aaaattgtcc gcgtcatcga acctcagaga gatgatatta tcgtcgaaat
     7501 tggcccgggc aaaggcgcat tgaccaggtt tctgctggaa tcaaacgctg aaatccacgc
     7561 tatcgagctc gatccagaat tggtcaatta tttaaaacaa gaattagggc aatttgaaaa
     7621 ttttcacctt tattatgccg acgccgtgcg gttcgacttt agcgaccttt tcaaccgtaa
     7681 tcaaaaaata aaaatcgccg gcaatatccc ttataatatc accagcccgt tgctttttcg
     7741 acttttcgaa ataaccgact ggctcgaccg gattgtctta ttagttcaaa aagaaattgc
     7801 cctccgcctt tgtgcccaac cggcaactaa agaatacgga attctttccg ttatgactaa
     7861 attctactgc cgaccgaaaa ttgagttcaa cgtttcacca caggtcttcc gcccgattcc
     7921 acgagtaact tccagtgtgg taaagctcgt cccaattgag aataattata gcccggaatt
     7981 ccgccgccag ttcaatgaac tggtccgaaa agccttcaac cgccgacgaa aaattttacg
     8041 aaattcgctc gatttaccgg cttcgactga tatttccgcc tgcccaattg acttattgcg
     8101 gcgcgccgaa acgctgagcg tggcggaatt tattgaattg acacactggt actatgcgca
     8161 ttttttcttg gataaaaagt gaatttggga taatttccag cgctttttaa taaacgaggg
     8221 cccctagctc aacggttaga gcaccggact cataatccgt cggttcgggg ttcaaatccc
     8281 cgggggccca caaaacctaa tcagacgact atatcgaact attggagata ttcttccagg
     8341 tattaatcaa atttaataat tcattatgaa cgttcgggaa acagataatt tcttcttcat
     8401 tttctccgag gattactttt ctgcgattcc tttattttac aacacttcga cttctatgcg
     8461 gtgagcgtga aatacctatg attcatttcc aactagcctg tagggcttga agttttgtag
     8521 taattattcg ctcaaaatat ttccccacct cggagaggtt taactttcta aaattgagac
     8581 caattttgat actacggaag gtttgtcgaa atgcctgcta tctaattata tttgccacta
     8641 tgaaaattca ttcgattaaa tctattatct ttgatcttga tggtactctg gttgacaccg
     8701 ccggtgatat tttagccgcg gtaaatttca caatgaaaaa attcagccta aaactaataa
     8761 gttacgagca atgtctgagg ttcctcggcg atggagtcga agacctgatc agacgagcag
     8821 tcgcaaattc actccgcgaa ccgattgatc caaactatct ggctgagatt gtcgcccaat
     8881 acaaggattt ttattcatct catttaaccg atcgctctcg tctatttccc ggtgtgcgcg
     8941 aaaccctggc cgaattacag gattatacat tagtagtagt ttccaataag agtcatgact
     9001 tttgtgtcca aatcttaaga caattggaca tcgagaaata cttccagcta ataatcgggg
     9061 gcgatttact accttcacgt aaacccgacc cggggcaatt gctttatgtt atgcaaaagt
     9121 ttaatctgac tgccgcgcaa attctaatcg tcggcgacag tgaaaacgat atacttgccg
     9181 ctcattcaat tggggcgccg gttgttgtcg ttaaatatgg ttatcgagat atagaaatac
     9241 tgcaagagta tcatcccgat tatatgattg acaatttttc tgatttatta aaaattctta
     9301 agcaagagat tcagacattt aaataaaccc gcgggatacg cggagcaacg gcacaacata
     9361 gttcatagat cagcatccca gccttcgcgg ctacttgagt agcggataat atgccatcgg
     9421 gtgaattgcc ccagatcaat acttcatcac caggcttgac atcggcgtcg ccgacttcta
     9481 ccatcaattg atccattgtg acggctccta ttatcggaaa aagctgacct ttgatcatta
     9541 cctgtccttt attggaaaag gctcggggca ttccatcggc atagccgata ttcaggacgg
     9601 caatacgagt tactttcttt gtaaaataaa gacgtccgta gctaatgctg gttccccctg
     9661 gcacttctct aatctgggca acccgagtct tcaaagacat tacctggcgg agagggatag
     9721 attcgctagt ttctaacgaa ggataatgac cataaagcgt aattccaggc cgcaccatat
     9781 caaaataggt ttccggcaga tccagaatgg cgccgctatt ggcggcatga atgagcgggg
     9841 aagttatccc agtttgacgt agttgcttta taatttgatt aaagcgggct aattgttcat
     9901 acgcaaaggt tttgtcacgg ctatcagcag ttgcaaaatg ggtacaaata ccttctattt
     9961 caatgttttt caattttcga gaggctttga taagatcaaa agcctgaata gccggtaatc
    10021 cgactcgtcc catcccagta tcaattttga cgtgcacttt cgcaactttc tgcaaagcgc
    10081 ccgccaagcg gtcataggtt acaaaatctt ctgcacgggt tacggttatc cggatattat
    10141 gcgcaatcgc gaaggatgct tcttcatcgt agagtgttcc gaaaatcaaa atttccccgc
    10201 cgatgcccga ttggattagt tccattgctt cgcacagacg cgctaccgca aagtattgga
    10261 caccttgatc tcgcagacat tgagcgatag ggaccgcgcc gtggccatag gcattggctt
    10321 tgacaacggc cataatttga gcaggtgcaa ctttttgttt tatattttga tagttatagc
    10381 gaagattatt gaggtctact tctgcgatgg ttgtgttttg cggaaaatgc atattctatc
    10441 caatcatatt tttttatcga gatggttctc atctttttaa tttttattta tatgttattt
    10501 ttaaatattc ttttaattca ttgaacgaag agaaaatttt agtagcacag ccgataatcc
    10561 agccactaat ttcgattacc ggaaactctg aaactaaata aaccggaata ttatggtaaa
    10621 aagcataggt tacctcgcca taggtgcctc cacccttcac ggcgtatttg tcccaattgc
    10681 aaataacata atctattttg gtttccaatg cccgtaaatc acctcgaatc aatttccgga
    10741 caatttgctg aaaacgcaaa ggatcagttt gtttatatcc gcggaaatta cgcgattcgt
    10801 caggtgtcaa atatttatgt tcctcaatca aaggattata acaggagtgt ccgaactctt
    10861 cctgtaaaaa cttttccatt tctgtccgcc agaccttccc gtggtcgggg gcgtattcaa
    10921 tcgcaccggc taaataggct atcaattttg caatttcctt ttaaaaaaga aaatcccacc
    10981 aataaaaagc aagatattga gcacaatcaa atataaaatg tttaggaact caaatggctg
    11041 acgcgttaaa atggatcgcc cgccttcgat caatactgtg gatggtaaag ccatcagcaa
    11101 tttacttata aatcctgtat gaaaatgggt tggaatgatc atcccggaca ttaaaaatag
    11161 gggcacgagt attagaaaaa tccagtaaaa ctgattacgc cgatcttctg ctaaaccaat
    11221 caacatacct aactggataa atataccaag cggtaatatt gcaaatatcc agaaaagtaa
    11281 tagtctgcct aatccgggga aaaaccccga aagtatcaga acggttaagg tcgtaacaag
    11341 cagatgaaga attagaatta tccacagtcg aatcagaaaa ctgctataaa tttgtgagat
    11401 tggcattccg ggaaggcgcc cgtaatcacc gcggctgtcc tgaaaatagt gcgtcacata
    11461 ggaactggct aatcctagcg ccatcagtcc gctggtaaat cctatgatcg cagacaatat
    11521 aaatttatca acctcgaggt aatcaattcg cggcagaatt ttccccaaac tggttaccag
    11581 agcaaaatac actaaacaag taccgataat attgaaaaat aagcccagca aaattttatg
    11641 caaatcaagg cgtaactcat tctttaacat tatcttctta aatatcatta ctaaaactct
    11701 tcatcagata ataaacggtt aataaattca gcgatactta gagactggat attcactccg
    11761 accagatttt cagcgggaat cgtagccagt acggcataga aatcttggag tctttcaaaa
    11821 gtcagctgga tggttaaatc cagatcatag actttagaaa aattatccga ttccttaatt
    11881 ttttccagta aagtctcatc ttttttgcgc aagataattt ccccggaaaa tggcacatct
    11941 actcgggtta acattttttc gagagtaccg tcgaatctaa ttttcccata atccagaaca
    12001 atccagcgtt cagcaagagt ttgaacttct ataaattcat ttgacgcgac gataatcgtc
    12061 ttgtggtctt taacggagag taagtattta aataacgatg tgcggttctg gtaatccaag
    12121 cctaaagttg gttcgtctaa aatcaatatc tgcgggtcac cgagcaaaac cagaacaagg
    12181 tcaagacagc gcttagcgcc gcgtgaataa gtcaccggat attcatccag cacttcctgt
    12241 aaattgaagt ctttgatacg aggctcacaa atctctttga aacgagactg aggtatttcc
    12301 agtagctcag cccgcgctct gagattttcc cagccggtaa tccagcggtt atgaatatcg
    12361 tgatcgggta ggtaaccgat acaattttta gccatttgcg gggatttggc aatatcttcg
    12421 ccgttgataa aaacttcgcc cgcatccggt gccataaaag ttgatattac tctcaaaaga
    12481 atagacttcc ctgctccatt cctgccaatt atagcaaaaa atgtattttt ttcgatgccg
    12541 agagtcagat tactcagaac atatttcttc ttgaaaaatt tgcttacatt tctaagcgta
    12601 atagaagctt ccattaatgc gcctcatacc atgaattacc aactccaata tctaccttaa
    12661 ccggaacatc cagaggtaaa gcgttttcca tttcggtagt gactatttga cgaagacgtt
    12721 ccaattcatc cggatgaact tcaaataaaa gttcatcgtg aatctggagt atcatcattg
    12781 atttgaattt ttctttagtt aattgacgct gaattctaat catcgcgatt ttgatcatat
    12841 cggcggctgt gccttgaatc ggcatattga ttgccacgcg ttcagcggct tcccgcacat
    12901 ttttattttc actattaata tcgggaacat agcgtcgccg accggttaag gtttttacgt
    12961 aagtattttg ccgggcttcg gcgattgttt ttgttatata atcattaatg ccgggataag
    13021 tgcggaaata ttgttcaatg agctgatttg catcggaacg tgaaatcccg agctcttccg
    13081 acattcgaaa tggaccggcg ccgtacataa tgccgaagtt aacgactttg gctatcctgc
    13141 gcatttccgg caagacatct ttttcgggaa cgccataaac cagggctgcc gtgcgggcat
    13201 gcacatctac tccttcctga aaggcgcgtc tgagttcggc atcaccggat aaatgcgcca
    13261 ttatccgtag ttcaatttga gaataatcgg ctgataaaat tttccaaccg gtttgctgag
    13321 gaataaacgc tcgacgaatc tcgcgaccaa cgtcggtgcg aatcggaata ttctggaaat
    13381 tcgggtcgct actactcaaa cgtccggttg ccgccatagt ttgattaaag gaactatgca
    13441 ctcgtccggt ctttttatta actaaaagcg gcaaagtatc caaataggta tttttcaatt
    13501 tcaccaaacc gcgatattcg agtacggcag acggcaaagg atgtttatgt tgcaatttct
    13561 ccagaacgct aacatcggtt gaatttccgc ggactttggg taagttcagt ttttcgaata
    13621 ggatttcgcc gagttgtttg ggagaattga tattgaactc gacacctgcc agatcataaa
    13681 ttcgtcgcat aatggcatca atttggactt ccagattgcg tgacatttcc ttcaaacttt
    13741 tcacatctag ataaacgccg tttaattcca gttgcattaa aaccggaacg agaggcattt
    13801 cgatctcttc aaaaacttgc cagagttgat cagcctttag cttctcttcg aatatcggta
    13861 ccagttgtaa aacaacatcg gcgtcttcga cggcatagaa tgtcacttta tcaatatcaa
    13921 cctgctccat attcaattga tctttgccgt ttccgatcaa ttcttcgatt gactgcattt
    13981 tataattcaa ataatactga ctcaatttat cgagtttgta agagcgagca tcgggttgaa
    14041 tcaggaaggc ggctaccata gtatcgaaga tcttgccgcg caattcaatg ccgttgcggc
    14101 gcaaaatcag catatcgtat ttcagattct gtccacattt aacgatttgc tcattatcta
    14161 aaagtggttt aagctccgct aaccatgctg aattatcggt ttgagcgaaa agctggtcag
    14221 ccattgccgg atgtctgatc ggcaaataaa ccccggagtg ggcttcccag caaagcgcaa
    14281 ctccgacgat ttcagccatc attggattaa tagaagtcgt ttccagatca accgataata
    14341 attctttgtt aagtaagttt tttaccagct cgcgcacttc cgcttgagtc tggatggttt
    14401 ggtaatgttt atccggtttg acggcctctg agacttcggg ttttgtttcg attaacgagt
    14461 agaattcaag ttcgcgataa atacgttcca gttccgcctg attgaccggt ttaattttta
    14521 acgactcaat attaatatcc agcggaacat cagttttaat cgtggctaat tgtttagaaa
    14581 gccgggcaat ctccgcccca ttttttatcg tttctgttag gcgagggttg ccgattgatt
    14641 cagcctgttc gatcactttt tcaagggagc caaatttagc caaaagtggt tgtgccttag
    14701 caggaccaat gcccttaatc cctggaatat tgtcggaagg atcgccgacc agaccgaggt
    14761 aatcagtaat ttggtcgggc tgaacaccta atttgctgac taccgcattt ttatcgagaa
    14821 tctcaacctc tttctgtccg gaagccggtt tatagaccaa aatatcatca tccaccaatt
    14881 gcataaaatc tttatcacca gtaaccacga aaacctgcaa gcctttattt ttggcggctt
    14941 tggcaagcgt accaatcacg tcatcagctt catagccagg tactgtgagc atcggaatat
    15001 tcatcgcctt aacgatttca aacataaccg gcaattgagc cactaagtct tccggcatcg
    15061 cttcgcgggt agctttataa tcggtataaa tctgatggcg gaacgtcttc tccggagcat
    15121 cgaaaactat agccagataa tcgggtttga cttcctcgag gatgcgtttc aatgaattaa
    15181 cgaaagcgaa taccgcgcca gtcggtttcc ctcgggaagt cgccaaacgg ctgcgaatgg
    15241 tcccaaagta agaacggtac gctaaagccg acccatctat taaaaataat gtttccttct
    15301 tctccataaa aatatggaaa attttaatca ttaactggca atttgccaaa cgatttcagt
    15361 gaaaagtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngga cttaaaagta
    15481 cttttaaaca cttttcttag gagttttggc aaagaatagg agaattatta atccggcaca
    15541 gaataatcca atagaagtaa actgggcgct ggtgagattc cgccaaacgc gcggattgac
    15601 tcggacaaat tcaatcagga agcgttccag tccggcgaac atcaagtaga ttgccatagt
    15661 ttgcccgact ttggttgttt tggggcgaat tacttttacc aagataaaat aaaaagcaaa
    15721 gttcaagaaa gcctcataaa gctgagtcgg atgcaccgga taattagccg gcggtgaagc
    15781 attaggaaaa gttactgccc agggtaaatc acaggcttta ccatggcaac aaccggcaaa
    15841 aaaacagccg atacgcccta taccctgccc gattaaaagc aatggaccaa tgatgtcagc
    15901 gtaaacgcca atactttttt gcgagcggcg taaaaccaga atcacggcaa ttgtcccacc
    15961 gatcaatcca ccgtgaaata caagcccgga accggaaaag atcatccctt tcggatcagc
    16021 taataaatcc ggaatatttt ccagtagata aaataattta gccccgacaa tgccaccgat
    16081 tgccgcccaa aatacaacat tactggcgaa atcaggatta tcgccgagtt ttttagtctc
    16141 ggctcgaaat atcaaatagc aaacaataaa agcgatcgcc atcattagac cataagtata
    16201 aatagtcacc ggaccgatat gcagaagttc aggatacata tttcattcca cctcgtgtct
    16261 attccaactg tttatgtgta ataaactggc taatggtctc ttgggaacat gaagagtatt
    16321 tttctcatcc agccaatagc gaatttcatt cctattttct atcacaggcg attcagccgg
    16381 atagccaaag gcaaccaccg agtcaatttc atattgctcg ggaattgcga gcaaagcccg
    16441 caatttttcg cgttcgactg aagcaagcca gcaagtgcca actccatttg cccaagccgc
    16501 cagaatgaaa ttttctatcg ccgcgccggc atctttagca taatgcgatt gcattaaatc
    16561 tttatttatc aaaaccacca aataaccggt cggatgttgt ccctctttgg gatcacccaa
    16621 tggtgcaatg taactcgccc agcgtaaaag tggaaaaact cgcgccctta attccggctc
    16681 ggtaatcaga aaatactcaa gtggttggag attgcgagcg gacggtgcta agcgggcgca
    16741 atccacccat ccgataataa tggcttgatc aacttgttcc tggcgaaact ggcggattgt
    16801 acggcgagtt ttaataatat tgataaaatc agaagctgaa gccataaaag accctttcgg
    16861 atttaaagaa tagcgttccg catttcgaga tcggtttgac gtaatttgcc ggtaagaatt
    16921 ttagcaatgt tgcgcataat taagtagcct acatgttcat tttcgtttaa ataccggtat
    16981 aaaacgacat tatctacaaa aatgagatca gtatcttctt cagccacaac tgttgctgag
    17041 cgtggtgagc cgtcaataag cgcaaattct ccgaaaattt cattatcacg cagggtggca
    17101 attttttcgc atttgccggg aagtgatacc gaataaatca aaacactcac ccgccctttt
    17161 gctataatat ataattcttc actgggttca ccttccctga aaattatttc gtctcgtttg
    17221 atgtgacgac agcgaaacaa accggctaaa gcctcaagtt cagcatctgt caacccatgt
    17281 gtcaaatccg aaactcgcag agtagtaatt atattcatca tccctcctgt gttattaatt
    17341 ttaggacgtc ttttctgcca ataaaacagt attggcaatc agcatagcaa tcgtcatcgg
    17401 accgaccccg cccggtaccg gcgtaatagc ggaggcaatt tcggctactt cactgaattt
    17461 gacatcgccg acccagcgat agccctttgg tgatgatgta tcttcaatcc gattggatcc
    17521 aacatcaatc accaccactc cgggtttcac aaatgaacga tcaataaact ccggctgacc
    17581 aagggcggca atcaaaatat ctgccagacg cgtaatttcc ggcagattcc tggtgtgcgt
    17641 atgacaaacc gtaactgtgg cattaccgag cggccgtttc tgcatcaata aattagcaat
    17701 cggtttgcca acgatgttac tccgaccagt tatgactaca tgtttaccgg ttgggtctat
    17761 atcggaatat ttcaaaagca ttaaaattcc atgcggcgtg cagggcaacg gcgcttcaat
    17821 ccccaaaacc attttcccca aattggtggg atgaatgcca tccgcgtctt tatcggctga
    17881 aatagcctga ataatggcat tactgtccag atgctttggc accggtaatt gcaccagaat
    17941 cccgtggaat cgcgaatcgt ggtttaattg atttatcaag tccagaattt tctcctgcga
    18001 actttcgccc ggcagacgaa tagtttcaga aaaaagttgc atactctcga aggctttggc
    18061 tttcatattg acataagact gggaagccgg attatcgcca actaatacga ccgctaagcc
    18121 tggcgtgatt cctttctgtt ttagacgatt tatacgcggt ctgagatcgt ctttaacttt
    18181 ttgggcgatt tcaacgccgt taatcaattt tggattactc atttggcaaa actcacacta
    18241 cgatgttcac gaacgacaac aacttttacc tgaccggggt aattgagttc ctgctggatt
    18301 ttggcggcga tttcttctgc caaattatcg gcttctgcat cgctaaccac cccattttcg
    18361 acgatgacac gtacttcgcg tccggcttga atagcgtaag tcttcgtaac cccggcaaag
    18421 gaagtcgcga tggattccag cttttccaaa cgctgaatat aactttccag cgtatcacca
    18481 cgtgctccgc gccgtgaacc gctaatctgg tcagcggctt gtacaatggc agaaatcgga
    18541 tgagtcgctt cgacttcctc atgatgagag gcaattgcat taattactat cggattttct
    18601 ctgaattttt tgacaaaatc cgcaccgatc aaggcgtggg taccagtgac atttcgatca
    18661 atagctttgc cgatatcatg caagagaccg gctcttttgg ctaattcacc atttaatccc
    18721 aattcagccg ccattaagcc ggataaccag gcgacttcaa tggaatgttt taaaacattt
    18781 tgaccatagg aggtacggta tttcagtttg ccgagcattt ttagcaattc cggcggaaat
    18841 tgagaaatgc caacttcatc aatagcctct tccgccgcac gcaaaatttc ctcatcaacc
    18901 tccttctccg ctttattaat catttcttcg atgcgcgccg gatggattcg tccgtcagca
    18961 attaattttt ccagagcgat acgggcaact tctcgacgaa tcggatcaaa accggaaagg
    19021 acaacggcct ccggcgtatc gtcaataata atctcgattc cggtcaatga ctcgaaatgg
    19081 cggatattgc gtccctcgcg tccaataata cgtcccttca tattatcatt cggcaagttg
    19141 actaccgaaa cagtcgtttc ggccgtatga tcagccgccg agcgctggat agccgagacg
    19201 ataatttttt tagcatcgcg attggcttca gccagcgcat tttcgcggat ttccttgacc
    19261 tttttattga cactttcttt agctttatca agcagattgt tcattaaaat ctcttcggct
    19321 tctttacggg acataccggc aatgcgctcc agtcgttcat tctgagcttc aataatattc
    19381 tgaagttcgg cttctttgtg gaggataatt tcgctatgtc tttttaattc ttcctcccgt
    19441 ttattgagct cttcggtttt tttatcacat aattcggttt tccggtcgag atagaactcg
    19501 cgcttggcga gttttttttc ggcttcgcgc acatccttca ttttgttgcg ggcttcctct
    19561 tcggcgcggt ttcggatacg aatggcttcg tctttggctt cgagacgagc ttctcgtttg
    19621 atttcttcgg cttctttttg agcgttggcg ataatttccg tcgcggtcgc ctgtgccctt
    19681 ttcaattttt gggtatatat gtaaagcgat aaggcagtcc caacaactgc tccgcctatt
    19741 gcgaagataa tataggtcca ggtcattaca cttctcctta tagaatcggt tagaactcta
    19801 ccgttaattt tagataattc caaccgtaat aaggatggta gagttaatct gatttattta
    19861 tttaagatgg attaaatttc ttctttaagt tgctcgtcaa tgatatcaat caagaaacct
    19921 gccttctcct cgacggagct gactatttta tctttttctt gattggctgt aaataattca
    19981 tcggtaatat tcattgcggc taaaatagct acacggatag agggttgaat agtacggagc
    20041 gtagattcga cttcctccat cttctcattg acatagttgg caacgctgat aatatagtcg
    20101 cgatcggctc tggactttac actgtattcc tgaccataga tagttacacg gactatattt
    20161 acgttaggtt cttccatctt ttttaaatca atttttgttt caaataacgt tacggttgga
    20221 cagggattaa taatccttta aaagttcgag cataccttcg attttagctc taagttcctg
    20281 tcttttttta aggtctaaca ttacttccga agaggctcct tcttttaatt ccgacttcaa
    20341 tttctcattt tcttctttaa gatgagttac gagcaacaac aaacttttga ttttttcttc
    20401 taataaattc aaattgacga tgttcatttt gctcttctca tcttaaacgg gcattcaatt
    20461 cccgtgataa attggcataa atagttgcca taatttgatc aacttcggca tcggttaagg
    20521 ttcgatcatt agcttgaaat aacaggttaa atgtaagact tttttccttt ttgtcaatat
    20581 tttttccaag gtatagatca tagaacctaa tttcacgcag taaatttcca ccgttttgtc
    20641 taatattttc aatgacttta tccacgggaa tcgtgcgatc tactataatt gaaatatcgc
    20701 gttcggtaaa cggaaaagct ggcggctcgt gatacgtttt agtcaaattc aaacttttca
    20761 ggatgacttc cacatttaat tccatactgt ttaccggtaa ctgaatgtcc cattccttag
    20821 ccagataccc gggcgctaat tcccccaaat aacccagttt agcctcacca gccaagattt
    20881 gaattaaatg cttgtaataa ggatttgcgg ctttttgaaa attgataggc tgaagaccaa
    20941 attgtttggc aatggcttct accagtcctc ttaaaaagaa aatattactg gcgcggcgtg
    21001 gatatgccca attcggctca tctaaatatc ccgttaaaat tactcccaga tgaagtgatt
    21061 cctcagccag agtttcggct tgcggtgcga aagcttgcac aaaacccagt tcgaaaagtc
    21121 ttagatcggt gcgatagtga ttgaaattat gtttaaccgc cgcaatcata ccaggtaata
    21181 atgaggttcg tagataagcc atatcctgac cgagtggatt acgcagtttt aacttatcac
    21241 cagattcagc tccaaattca gccgctttag cactaaccaa agaattgcaa tacaattcgg
    21301 aatagccaca accggctacg aaagaccgca acttttcaat caaatcgtaa cgctcgttga
    21361 tagtttccat cggaacaatc tggatatatt tagccgacgg tactttgctc attccataaa
    21421 cgcggacaat ttcctcgatc aggtcaactt ctcgctcgag atcaggccgc caggtcggga
    21481 cagtaaccgt gatttcactc tcagcgctat taataatttc gcaacctaat ccagttagta
    21541 cttttttgat ccaatccggt tcaaaattca tccctatcaa acgattagta cgttcataac
    21601 gcaaaacaac tctgcgagct ttaatcggat tgggataatt atccacgata cctcggcaaa
    21661 tctgaccgcc cgctacttct acaattaggg ccgccagtcg gttcagcgca tagaggagac
    21721 cttcataatc ggtgccgcgt tcaaagcgat aagacgcttc ggtttgcata cccagaaaac
    21781 gcgctccttt tcgaatcacg cctggattga aataagcact ttccagcaag acatcgcgag
    21841 tatcatcatt gatctcgcta ttcgccaaac ccataatccc ggcaattgct accggctttt
    21901 cgctatcgca aattaagaga acatcgcccg aaagttcgcg ttcaactcca tcaagcgtaa
    21961 cgactttttc gcccggttta gcccgacgta caacaatttt ctgccctttg agatgggcat
    22021 aatcgaaagt atggagcggg tgtccggttt ccattaagac ataattggcg gcatctacca
    22081 cattattgat ggaacgctgt ccaatacttt ctaaactttt aactaaccaa tccggtgagg
    22141 gaccgatttt tacaccgcga attaggcggg cggcatagcg cgggcaacca accggatcgg
    22201 cgatttcgat ttcgatggaa tcgctagttg gttgctggat ttctttgaga tttatggctg
    22261 gcttgcgtaa ttcattgccg gtcatcaatg cgatttcacg agcgacccct atatgcgatg
    22321 cgcaatcagg gcgattggga gtcaggtcaa tttccagact ctttccatca gtcgccaaat
    22381 aatcagaaag gcgactacca accgccgcat cttgttccag gatcataatc ccactatgat
    22441 cgttggaaaa ccccagttca tcttccgcac aaagcatacc ttcggagact acacctctga
    22501 ttttagttgg tttaagcgtc ataccattcg gtaatgtggt ttccaccaat gccagcggga
    22561 ctttttgccc ttgtttgaca ttaggcgcgc cgcagactac ttcgaattgt tttgctccag
    22621 ttgaaacggt gcaaattgtc aagcgatcgg cattaggatg cggtcgaacg gctaatattt
    22681 cagcgacgat gatattttct aaaccggtgc ttttatcctc gattaaattg gcttccaagc
    22741 cgatggcagt cagacgttca gctaattctg ccgggctgaa gggaaaatcg gtgtattttt
    22801 ttagccagtt tgtattgact atcataaggt taaaactgtt ccagaaagcg catatcacct
    22861 tcaaaataaa ggcgaatgtc atttatgccg tattttaaca acgccgttcg gtcgattccc
    22921 atgccgaaag cataaccggt atatttctcc gggtcaatgc cgacattgac aaaaacgttt
    22981 ggatctacca taccgcaacc gagaatttcc aaccagccag tatttttaca gagtcggcaa
    23041 cctttaccgg aacaaatgaa acaggacaca tccatttcgg cacttggttc agtgaacggg
    23101 aaaaaactgg gacgaaaacg agtttttacc tcgctaccga aaaagcgttt cgcaaacatt
    23161 tcgagtgttg ttttcaattc gctaaaggtt acattttcat caacgtatag tccttcaact
    23221 tggtgaaaaa cacaatgact gcgcggattg atggcttcat tccgatatac tcttcccggg
    23281 gctaaaatcc ggatcggtgg tttttgcttt aacattacgc gaatttgaac cggtgaagta
    23341 tgggttctca gcagtctttt ctcgtcaatg aagaaggtgt cttgcatatc gcgcgctgga
    23401 tgattttccg caaagtttaa agcttggaaa ttataataat cggtttcgac ttccggccca
    23461 aattcgacac taaaaccgac ttctcgaaaa atagaaatga cctggttgag tgtttgggta
    23521 atcgggtgta aatggccaac ggcaggcgcc agacctggta aactgtaatc gaaatattcg
    23581 tcctgagcga cctgctcctt cagtcgttca tttaaagcct gaatctgagc ttcagcttct
    23641 ttcttcaaaa tattgagcac tttaccaacc tggcgttttt gctcggcggt aatcgaattg
    23701 aaatcggtaa aaagcgccgt tatcagacct tttttaccta aatatttaat tctgagattt
    23761 tcaaccgctt gaggatcggc gctgacctgc tgaagatctt tttgcagttg cccccgaacc
    23821 tcatcagccc gattcaggat gtccatactc tcagcctttt accagcgcta ccaacatcgc
    23881 aaatccatcg gcgtcgtgca tggcaatttc ggccaaagct ttacggtcga gagtaatccc
    23941 agccttcttc agaccggcga taaatttatt ataggaaatg tcatgttgat tagcggcggc
    24001 attaatgcgc gcaatccaga gggagcgaaa atcgcgtttt ttattgcgac gatcgcgata
    24061 ggcatattgt aaggcttttt cgacggcatc tttggcacta acgtaatttt tactgcgtgc
    24121 gccacggtag cctttagcca tcccgacaat tttacgatgt tttctatgat ggggaacgga
    24181 actatttgct cttggcataa ttttactcct atactccaag catcttttta acgcgtttaa
    24241 cgtcagcgct tttaagaatt tcagattgtc ttaaattgcg tttgcgtttg ctggattttt
    24301 tagtcaaaat atgagaatga taggctttgt tacgtttaac ttttccactt ccggtcaagg
    24361 taaaacgttt agccgctgat cttctggttt tcattttagg catatttttt tctcctattt
    24421 agccgtgaag taagtaacga tggttcgccc ttcttggaca ggattttttt caggtgttcc
    24481 gacgtcagct agtttctcga taattttttg caccagttct tttccggttt cctgatgagc
    24541 catctcgcga ccgcggaaca ttagagtgat cttgactttg taaccttcgc cgaggaatcg
    24601 tcgggcggcg ttgattttca tttcaaaatc atgttccccg atatgcggtc ggaaacgaac
    24661 ttccttaatc ttaacaacat gctgtttttt cttactctca cgctctttca tttgttgttg
    24721 ataacgatat ttaccaaaat ccattatctt acagacgggt ggattagtgt tcggtgatac
    24781 ttccaccaaa tcaagcccac gatcttcgga tattcgtaga gcttctttat tactgacaat
    24841 gccaatctgt ttcccatcaa agtctattag acggacagtt tggcttcgaa tttgttcgtt
    24901 tacccgtact tctttctcta tgacttgctc tccttcttta attgaatttc ttttacaatt
    24961 aaatcaatgg cttcttccca gctcattacg ccggtatcgc cgatttttcg acgtctaatc
    25021 gaaatctgtc gtccagtggc ttctctttct ccaattataa acataaatgg aattttcatc
    25081 agctcagcat cgcgtatttt agcaccaatg gtatcatttt gcaaattgat ttcagctcga
    25141 attccattgt caattaataa tttataaaat tctgttgcga tttgatcctg attatttgtc
    25201 accggaatga caactgcctg aaccggcgcg atccatagcg gaaaatctcc gacggtatgt
    25261 tcaatcaaaa cgccagcaaa tctttccagc gaacccataa tcgcgcggtg aatcatataa
    25321 ggccgacgtt tattgccgtc actggcaata taatccatat tgaaacgttc cggctcgtta
    25381 aagtcgaatt gaatagtcgt acattgccag gaacgattca aagcgtcacg gattttaatg
    25441 tcaattttgg gaccgtaaaa agcgccgcca ccttcgtcta tgtgatattc aatatcgagt
    25501 ttacttagtg ccgctttcaa agcctcggtg gcttccgccc aattttcagg cgaaccgaca
    25561 tatttttctt tcgggcgggt agcgacatat acatccattt ctttaaagcc aaacgagcga
    25621 atgaattcaa atgagaaaag cactaaactc tggatttcat cattgagttg ctccggcgtg
    25681 caaataatat gggcatcatc ctgagtaaag ccccgtacgc gcattaatcc atgtaacacg
    25741 ccggataatt catagcgata aaccgtccca agttcggcat aacgaatcgg caggtcgcga
    25801 taactacgcg ttttcgattt ataaatctgc atatggaacg gacagttcat tggcttgata
    25861 tagtaagtct cgccttcgac tgtcatccca tcgaacatag attcacgata aaagtcgagg
    25921 tgaccacttg tttcccaaag ctgagctttc ccgatatggg gcgtttgtac tagatcgtag
    25981 ccgcgtttta aatgttcttt aatccagtaa tctttgatat tttgcaaaag caacgcacca
    26041 cgcggatacc aaagcaccag tccggggcca acctgttcgt tgatttcgaa aagttcgaga
    26101 ctcttgccga gtttgcgatg atcgcgccgc ttggcttcct cgataaaatt caagtacttc
    26161 ttaagttcct tttgagtggc aaaagaagta ccataaatac gtgttagcat tttattgcgc
    26221 gaatcaccgc gccaatatgc gccagcaaca ttcaataatt taaaggcttt gattttgccg
    26281 gtcgaaggaa tgtgtggacc tcggcaaaga tcggtaaaat cgccttgagt ataaattgac
    26341 aatggcacat ccggttcaat ctcacgaatg atctcaactt tatatgtctc acccatcctg
    26401 gaaaataatt caattgcttc ctcgcgggaa acctcacgtc ttataattgg caaatctgcc
    26461 gcaatgattt cattcatccg ggcttcgatt gctggcaaat cgccatccgt taaaggcggc
    26521 tcgatatcaa aatcatagta aaaacgattc tcgatcgccg ggccaatggt aacttttgcc
    26581 tgtggatata attgcttaac cgcctgcgcc ataacgtgcg aggtactgtg cagtaatatt
    26641 tcttgtgatt cgggagaatt ataggtcaga atctcaagcc gagcatcttc cgaaataata
    26701 gtcgaaagat caaccaagcg gtcattgact ttagcggcga gagcgttctg aaaaagaccg
    26761 ggactgattt ctttagcaat ttgcccggct gatacacctg ccgacacttc catagttgaa
    26821 ttgtcgggta aatgtatcgt aatctttcga tttgattcca aaatttgctt ccttttcgcg
    26881 cttcagagtc ataatgacta tatttaattt ttcaattgta ctagggcgat actggactct
    26941 tccgacccat cggggttcct aataaagaac cagtgtcccc ggtttttatc aggatagata
    27001 tcattaacca tctaacctga attcgattgt aaaaaaactc actttggtgg gcgatactgg
    27061 acttgaacca gtgaccccct gcatgtcaag caggtactct agccacctga gctaatcgcc
    27121 cggatatatc aacttaaaat aatggtccaa aagttgataa taattattcc aattcattat
    27181 cgtcaaaaaa cgaatgcaaa tttaaccaaa gcatatttat cacgcaaaaa gaaaaattga
    27241 cattgaggtc cgcttaatac ataattctct gtttaataaa aatactaaac gattttaggt
    27301 agtgaatttg ttattttatt aattgattca aaaaactgct ctgcgttaat cctatttatt
    27361 tctaaaaggt ggataccatg tcttaatact ttacataaaa aaccgattca ccccatgggg
    27421 tgaatgtaaa cgatgtcatg ctatgtaact attagctctt ttcttcattt tttttctaag
    27481 gtattttcaa ataaaagaat tgttatgttt tgttatgatt cacttggaaa tcgaaaatta
    27541 ttaagtttga aataagagtc aaaaagaaag gaaatcaaga tgaatcaaag tcgtaatcag
    27601 aacacagccc cagcaagaag gttgaaattt taagggagca cctggagaac catcccgaac
    27661 attcgggaca ttagtgagat tgctcgtcgc tacggaatca atcccagccc gttacatata
    27721 tgaaagaagc agttatttga aggcgctgtt gaaacatttg gtcgtcagca tgaaaaagcg
    27781 agactgggca atctgccagg aaaggacaat tagaggaaaa gattaaggac aaggatagtt
    27841 tgattgccga acaaatcgct gaatatattc ggttttataa cgaggaacgc ttgcatagcg
    27901 ctctgtttta tctgacacct aaagaagtac ttgaaggtaa aatggaacaa cgacttgaag
    27961 aacggcaaaa aaactagatc aagctgccga cccgcctgct cccgacatag gagggcgggc
    28021 aggaaatcgt aaaaatttat ccttatttga aaggagcgaa aattccattt ccttatgaac
    28081 caagacatgt gaaacttcca accatcccta tgaactatgt ctggaattgt cggatattga
    28141 acatcgcaat cccgcctggg cgggacgcta tccgcagacc aacggatttg tataacgatt
    28201 caatcgtact gccccaaatg aattcttcta accagcctac cggctacgtt aatgttataa
    28261 atcgcggacg atatccagac cttgcggctg gggcataacc cagcgaacag gctgaatcgt
    28321 tccatctttg cggactgtac gctccacccg tttacatttc aataccgaat gttcaataaa
    28381 caggtcgtct tcgataattg acccaaatat ataagtcgtc cctttgatga caacattttt
    28441 cccgatgata gttggatatt cgctactgcc ggtaataatg acgctcgatt cgatgctcgt
    28501 attatcaccg attgtaatgt taccttttat caaatcaccg cgcataatga cacaattttt
    28561 cccaatagtc atttgttgat tgggataggt gctaagagaa acattttcct gaatgaccgt
    28621 gccaacgccc agatggatgt taccattaag taaagagccc gcccgaattt ttacgccacg
    28681 ttcgaggtgc acaccactcg aaatatgcac acctttgccg atctcaatgt cgagcggcgg
    28741 attgatttta tccatttcca aaatccgaat aaccacctca tcggcaatga aaaaatcatt
    28801 ttcatcctcg atataaatta tatcccgcag aagtttccac acctttcggc gggcgatatt
    28861 ttccatttcc tgtaaaacac tcttgacgtt aaaaccgatt actgcacttt cgtcgtcaac
    28921 cggataagct tctacttcaa ttttattagc attgaaaaga ccaattatat cggttaagta
    28981 taattcaccc tgcacattat ccggagtaag ttgatggata aatcgctgag cgaggtcaaa
    29041 tttcatcgca taaacgccgg tattatattc cgtgattccc agcaattccg aacgtttaaa
    29101 ttgatatttt ttaccattat acacagtcgt ataaaatccg ctttcgggaa ttgctagaat
    29161 atctttatgc tccttaattt caataacgcg tccctcatct tctcctgagg gctcaccggt
    29221 agctgttctt gccgggacgc ggataattct accgtagcta ttagcttccg gttcaccttt
    29281 ataatttccc gttagcaaag ctaaaccaca accggattgt tcaaaacggc gtttaagttc
    29341 gctgaccacg gctggtgtaa tcaatcccat atcacccggg aagatataaa tgtcttttat
    29401 gtggccattc tggctaatgg ctgaaagtgc ctctttcacg gcatgcccgg tacctttttg
    29461 ttcggtctga tagacaaaaa tgcgatttgc tcttttccct gctgattccg ccacttcaca
    29521 ggctttaata cccacgacaa ttatctgatt gttagaagcc aagcctttac tggcggcatt
    29581 agcgacgcgc accacagtcg gcacacccca gatctcgtgt aacattttag aacgttcgga
    29641 tttaattctt ttgccatgac cggcggctaa gataatagct accgaaccct gtttcggatc
    29701 tatttcacca ctcaaactat gtaaatattc aaccagatcg ttattgccat tgtttattga
    29761 cattttcgtc tccgtcaatt tatctccgtg tgtgaaaaca attagatttc ctacaatatt
    29821 gtaataatat aaagaaaaat aaccggcgcg gcgataaaaa atgaatcaaa gcgatccagc
    29881 accccaccat ggccgagcaa taaattggat gaatccttga cttcggcgtc ccgcttaaaa
    29941 gccgattcga caaaatcacc agcctgtccg aatactcccg ccagtatacc aaaaataatt
    30001 aattgcatta tgctcataaa atccggcgcc caacctaaaa aataaaaaat gagtacggtc
    30061 agcacagcac caaatagtcc gccaatgcaa ccggcatagg ttttattagg actaatttta
    30121 ggtgcgatct tgcgacgtcc cagccacttt ccgattataa aagccagtga atcacaaacc
    30181 cagatcgtaa caaacaaaag aagtacaatc gaacgccccc taccaggaat attgcgcatt
    30241 aaaattatag tcgaagttaa caccggtaaa taaacaaaac cgaacaaggt actggcaaga
    30301 tcaaccgtta cgtttttgat ctggcgcacc aaactagcta gtaagagcaa aactaccaat
    30361 cctatcagta aataaagcag cagtgcgccc ggaagataaa aactggcaag ttgccaaatt
    30421 actccggcta aaacaccagg caaaagtagc ggcgcaattc ctttttttcg catcaaaaga
    30481 taaagttcaa cctgcgcgat gaaaaccatt accgttataa aaagaacgaa aggaattcca
    30541 cctaaatgaa tcaaaagcaa aatggctgga attccaatta tattgattag catacgagaa
    30601 gcgaagttat tcattaaaaa aacctgattt cattgtttcg gggaatcatt atttccccat
    30661 ttaagaactt taactttcac cttgccggta ccgtcattga ctaacccaat ttttcgggcg
    30721 gcagcgtacg ataaatctaa atcgcgaccg ggcacaaatg gtccgcgatc attaacaacc
    30781 acagtaaccg attgaccagt tggtggataa gtcacttgca gaaccgtccc aaacggcaaa
    30841 gatttatgag cggctgtcat tccgttcata tcgaaaactt ccccactgga agttaattta
    30901 ccatgaaaat ccgctccgta atatgaggca gtcaaaacgg cggtcctgtt agaattcttg
    30961 agcaagagca acgtattctt atcgtcattt gtcgcaccag ttttaggtga agtatagagg
    31021 taacgcggcg cggaagcaca acccataaag cccaacatca taaacagtaa taaaatgacg
    31081 attaggctta gacttcgact cgtccgatta tatctcgcag acaacttatt ttattctccg
    31141 tacaatattg ttcaatgaaa tcaagcattt cgccacagac tttaggatta aaaaaattgg
    31201 ctgtcccaat ctgaaccgca tgcgctcccg ccaataaaaa ttcaatcaca tccgccccgc
    31261 aactgattcc tccaattccg ataattggaa tatcaacttc tttgtaagtt ttaataatat
    31321 gggcaagggc gatcggcttg atcgccggtc cggaaaagcc accaactacg gtattaatct
    31381 tcggacgacg tgtatgaata tcaatcgccg ccccgtaaac cgtgttaatc atcgaaagag
    31441 catcagctcc gttttcctga gcggctaaag ccagaacccg gatgtcagtg acattcggcg
    31501 agagtttaat tattagaaat ttatcggtag catgacgcag tttcttgacc aattgtccca
    31561 gaattaacgg gtctctcccg aattcaatgc caccctcgct aacattcgga caggaaacgt
    31621 tgatttcaaa agccgctacc cattggcgat cggacaattt tttaaccacc tgaacatatt
    31681 catcatcttt gcgaccggct atattgacaa ttactttgcc gttataagcc gccagaaatt
    31741 cctccttctc ttccagaaat ttatcaactc cgatatttgc caagccgatt gaattaagca
    31801 ttccggaggt cacttcggca atacgcggcg gcggattacc aacgcgaggc tcgagcgaga
    31861 ttgatttggt cacaattccg ccataaagct ccggattaat caaatcggct atctcatcgc
    31921 cgtaaccata agtaccggaa gccgttaaaa gtggattcct aagaataaaa tcttctctta
    31981 actttatatg taaatcggtc atcctaagtc caattcattg gcgttaaata ccggaccatc
    32041 ctgacaaacc aaacgcagag gttgttccga cggatgagaa tggggaattg cacatccctg
    32101 gcagattccg agtccgcaag ccattaattt ttccaatgac aattgtaaat caaagtggtt
    32161 ttcgcgagac aaatgccata aagcactcag catcgcttcc ggaccacagg catatacact
    32221 aaatggatgc ggaatcgctc ccagattatt ccggagacaa tctactactg taccgcaata
    32281 ctcgactgac ccgtcttcgg tcgctatata tttttccccc gataataatg ataatggtgg
    32341 taattcatct ttagaacgca cccctacaaa attccagatt ttgtaattca aattagccaa
    32401 tttttggttc aggaaaagta gcggtgccaa accgactcca ccggctatca atacatgatt
    32461 gttatatttg atattgaagg tatttcctaa aggacctaga actcgaattt tctggctggg
    32521 ttgccattga ctcataatag aggtcaggca cccgactttt ttataaagaa tggttagatt
    32581 ttctcgattc actcccgcaa tgctaaaagg acggggaaac aattgcgggc gttcaggaaa
    32641 ataaaagttc acaaactggc cgggttgcgc cgactgactg atttcgggaa acgcaaattc
    32701 cattaaatag gtatcggtag cgaccatccg gttagataaa accgtggtat cttttaatac
    32761 tctcattcaa ccttttccgg cttttggggc tcattttcct cggcttcact cccctgagct
    32821 aaggcatctt ctatttcctt gagttgatca taagcatttg ccattatttg gtaatattgc
    32881 ccattgggat aagtgtcaag gtaatatttg taccaggtta aagcattatt caagtcaaat
    32941 aatcggtgat gatagaaata ggctaaatac ataaccgctt tctccgcggc ttggcaatcg
    33001 gtatattcct tggcaagctt ttcgtatatt gttatcgcag aatgcagatc agtctggtct
    33061 aaattttctg cttttaccaa taatgaatcg gcttcgactt cgcgggcgga agtattaagc
    33121 ggccgtttct caatttcggc taaatagatt gactccggga accactgctt aatttggtta
    33181 gcatattcgt tagccttttc gatattttgc cggtcgctgg caattcggta aagactgtag
    33241 agtgctttat ctcgtaaaga attaaaaggg atttcgtagg caactttttc aaaatagtaa
    33301 atcgcgctat cgggttgatt aaaactaaaa tagaatattt cagcgatttc ataaagggta
    33361 gtgtaatatt ctttgaaaac cgccagtgta tcgattgttt tcttctgggt tgtgtttttc
    33421 ttttcaagcg ctttcttcaa ttccgattgc tcctgatcaa ctgggagcga ctcgccgagt
    33481 gtatcgcttt cagtcggcgg attcagatta atcgcggcaa cctgttcact tagagcggca
    33541 agacgattat aaatagtatt aaatcgtttg atttcagaag atttattgcg acattcaaaa
    33601 gcccgcaggg tattaacctc gattttgacg acattatcat attgaacagc ggctttggta
    33661 taatcatgaa tttgagtcat atacaactcg gctaagtggt agctggcttc cgccgcaacc
    33721 ggtttacctt tataatcctc aattaccttg gtcaatcgag agattgcggc atcgtaatct
    33781 tgttgagcaa gataaatcaa tcccaattgt agctccagat caccccagat tttctcatta
    33841 gccggatcgt ttaatttttc attaattaaa tcaatggctt tatcatattg accggactct
    33901 cgataaattc gcaccaggag aacctgcatc ttatcgcgta agattaaaga aggcgaaaac
    33961 tggtcaattt ttttaagatt tttaatggct tcttgtggtc tgtttaaagc caggtcaatc
    34021 tcggcaatac gaaactgtgc ttcggcggca atatcgaagc ttcccccaac ttcagtcgcc
    34081 ttttgataat aaaataaagc cgagtcatag gcactgtctt taaaatacaa ttcaccgatt
    34141 gataaataga gctccgctag aagataataa tcatccgtaa ggttcatgat ttttttccac
    34201 tggaaaaaag ctaactcgcg ttcgtccaat ttccacaaat tacgcccata ccagaccatt
    34261 gcttcaggat ataatttgct ctgaggaaat ttgacagcaa aatcctcgaa cttctgacgg
    34321 gcaattcggt aatcgccttt ataataatgc gacattgcga ttatatagta ggaatcatca
    34381 acccagcgac tctccggata ttgagtgatt accttattag ccttttctat cgcggtatca
    34441 aattttttct tcaatgccgg gctgattttt tcatcaggaa gggttttaaa ttctttttcc
    34501 gcttcacgaa aatatttttc ggcattgtaa taggtattgt aataggcaca attcgacaag
    34561 accaatatca gccctattaa aaacagggtt tcgatagatt ttaccgtgat attatttttc
    34621 atatttttct ggcaaactca gattaatatc aaacctgcat cagcagatat tggtactccc
    34681 gattaaattg gggcaaaatt tcatcagcgg ctccttttag gaataaatcg gtaaaaggag
    34741 tcaataccgt ctcaacaata ttaatttcta ctaaaaaggc accattttct ttagcaatta
    34801 atggcagatc agccgcaggt cgtacctgag cagatgtccc tactgagaga aaaaggtcgg
    34861 cttgttgaga agcacgatat gcctcttcgt aagcttgttt cggtaaagat tcaccaaacc
    34921 agactacgtt cgggcgaata agaccgccac aataacagcg tggaatatgg tcaaatgaac
    34981 tattataaat ttcatcaaat tcggtggcgc tatattgctg accgcatcgg atgcagtaat
    35041 tttccataat cgttccgtga agttctaata catcgcggct acccgctcgc tggtgcaaac
    35101 cgtctatatt ttgggtaact aaagtaaatt ttgacaccat tttcgccaat tcaacaagag
    35161 cataatgtcc ggcattgggt tgacaaactt cgataatttc acggcgatgt ttataccact
    35221 cactcaccat agctgtattt aaataaaacg attcaaaact ggctaattcc tgaggagata
    35281 gtttagtcca taaaccgttt tccccgcgaa aagttgggat gccgctgggt gccgatatgc
    35341 cagcaccagt taaaaccacg acttgttcgc cttgactgat ccgttttcga gcaacttcat
    35401 tggtaattaa attcatcaat aaccatctat atttacttta aaattataac agctcgctta
    35461 aaatttagat tcaattattc atcaaaccaa tgaattaatc catcttcgaa aggataagct
    35521 aaagccggat gtttgggaat aatccggata gtgtagccgt tcaaaccgct ttcccaatta
    35581 ttgattttat caacgtacac gtattttttg gagtcaactt ttgtttcaaa tttcattgaa
    35641 ctgaatttcc cgtcaatgat gtcgccgtta ggatcaacgc ggccataata aacctgaaca
    35701 tccacatcat caacctgcaa accgtccaaa tatatctccg ctcgaatctc cagctgacta
    35761 tttaccgata attcttcgat tgacccggag gtaactttta ggaagcgaac caagtcccaa
    35821 tttttacgca ttttctcttt ccagtgtgcc aattctttgg cgactgcata gttatccttg
    35881 gacaattcat tggctctcac ggcggctgga tagtaaatct gatcaaggta ttcacaaacc
    35941 atacgattgg agttatattg aggacagatg gctttcattg atgctttgat catagcaatc
    36001 caatcacggg gcaagtcatc attatcgcgt tcgaaaaagc tcggaataat ctcgttttcc
    36061 agaacattgt aaagaatatt agcttcttga cgatcccaat agtcaatatc ctcataaatg
    36121 tcacgagttc cgatcgccca gccgattccc ggagtataga cttcatccca ccagccgtca
    36181 attgtactga aatgtaaacc gccgttagcg acggctttca ttccactggt accgcaggcc
    36241 tctaaaccac gacgcggagt attcaaccac aggtcacagc cctgtaccaa ataacgagcc
    36301 atattcatat cataattttc cagaaaaact acgcactgat aaaatggttc ttcctgggat
    36361 agtttcaaaa tacgccgaat aacctgttta ccttcttcat cttggggatg agctttacca
    36421 gcaataataa tctgaactgg aaaattttta ttcgtcaata tcttagctaa tctttcggga
    36481 tcagtgaaga gcagataggc tcttttatat gcggcaaaac gccgtgcaaa accaattgtc
    36541 aaagcgtccg tgcttaaaat actttttact cgcgcaatta tattttgagg tttaccgaga
    36601 gtaacatatt gccgatataa ttcactgcgg gcataagcta ctaaacgttc ccggcgacgt
    36661 tcatgagtgc gccagagttc gccgtccgga attttatcta ctttattcca gatagtctta
    36721 ctcgccggtt cacgtcgcca atccggtccg agataacggt caaataaatc agacatttct
    36781 tgtgaaatcc aggaggcttg gtggattccg ttagtaacat agtcaatggg aatttctgta
    36841 gtgagaatat ccggccagag agattcccac atttcctggg caacctgttg atgtaattta
    36901 ctgacagcat tgatcttact actcaaattt atggctagaa ttgccatcga aaaatgttcg
    36961 gaattatcgt ccggatcgtt acgacccaac gctaaaagtt cattaatttc tattcctact
    37021 tcccggcaat actcatcaaa atagcgtttt atcagatcag ggtgaaaaag gtcaattccg
    37081 gcttgaactg gagtatgggt ggtaaaaact gaagccgctt taataatttc aagcgcttcg
    37141 taaaatgtca gaccgttgtt ttttacaaca ttccgaatcc gctctaatcc cgaaaaagcc
    37201 gagtgccctt cattcatatg gcaaataaat ggcgtggcat cgagtatttg aagagcctga
    37261 aaaccaccca ttcctaaaat aatttcctgc tgaattcggg tttcggtatt gccaccgtac
    37321 aattcagctg taatcttttt atctgcttct gaattttcgg caatattggt atcaagtaaa
    37381 tatagcggaa cgcgaccaac ctgggctttc caaatccgag cataaaccaa acgaccggga
    37441 aaaggcactt taatcagtaa ttgtttacca tcagaacctt ttaccagagt cagcggtgaa
    37501 ttataaaagt cattgacagg gtattcttct tcttgataat catcaacatt taagacttgc
    37561 tgaaaatagc cgtactgata tgaaagtcca acggcctgga gtggaatacc aagttccgaa
    37621 gctgatttaa ggtggtcgcc ggctaataca cccagtcctc ctgagtagtc cgccagacat
    37681 tcggtaatgc catattccat cgaaaaatag gcaatattga ggtgggaatt gtatttgccg
    37741 ctcttttcga accaggttgg tgagtttaag gactgtttga atttgtcata aacccgacgc
    37801 agttggtaca aatagctatc atcttgggca cgcgtttcga gtacatcttg ctctactcta
    37861 ttcaatagag ccactggatt atggcgcgtc tcttcccaca aatccatatc cagccgccga
    37921 aagagatcgc gagcatcggc attccaggtc caccacaaat tataagccag atcacgcaat
    37981 tcttccaatt tcttaggcag aattggtttg acagtaaagg attgaatagg cttcattaat
    38041 ttcctcagct ttaaaaatca gtgcctaaat taatcaattg tcttctttcc tacaaaataa
    38101 tgaaaacaac aacaaagtaa taaaattcga tagaaaaacc actattgttc gcgattaatt
    38161 tcggctccca gttgccgaaa gcgtgggacg atatcttcat aaccccgatc aatcagttca
    38221 gccttctcga ttttactttt acctcgggcg attagggcgg caattacaag ggcaatacca
    38281 gcccgaatat caggactgac aatttcctgc cctttaagac gcgtcggacc tgaaacaagc
    38341 acgcggtgcg ggtcacactg cacaatttga gcgcccatcg tcaccagttt gtcaacgaaa
    38401 aacatacggg attcgtacat ccaatcatgg cagagagtcg tccctctggc ttgggttgcc
    38461 aacacgacca tcggactcat tagatcagtt gggaagcccg gccagactcc tacctggact
    38521 ttcggaatgg ctctgatatt tttggacggc ttaatcacca acgttttttc atcaaggaag
    38581 gataacttta ctcgcatttg gtcaaggata agattgatga tcgtcagatt ttcacgattg
    38641 gcatcgtgaa taacgacttc accgccggtg caagccgcgg caatcgcgaa cgttccagct
    38701 tcaatatgat caggcataat gcgatgtcga aatcctttta gttttttggt tccggtaatt
    38761 gttaagcgat tggtaccggc gccttgaatt ttagcgccca ttttcactaa aactttagct
    38821 aagtcttgaa catgcggttc tccagccgca ttatcaaggg tagttttccc ttccgtcatt
    38881 gccgccagca tcatcgcatt ttcagtagcg gtcactgaag cctcacgcag aaaaacataa
    38941 ttaggtttag ggcgttttaa tattagccta tagccgtgct caattatatt gacttttact
    39001 cccaacgcct gtaacgcgtc gaaatgttgg tcaactttac gccgtccgat tacacaaccg
    39061 cccggcggta ccatttcaac ttcaccagta cgcgccagta gaacgcttaa atatagaatg
    39121 gaagcgcgta gagtactggc taatttatca tttaggcgcg atttattgac cgatccggta
    39181 atttgacatt tatgagcgga tagcatctcc accgacttcc ctaaatcgct caggattgct
    39241 agcattgaac gaacatcgct aatatcgggt agattttcca gaatacaggg ttcatcggtc
    39301 aaaacggtcg cggcgataat cggcaatgcc gcatttttat tcccggctac cttgatctca
    39361 ccggataagt tgcgattacc ttcaatgata aatctggaca taattacctt tcattacgtt
    39421 aaaattttcc aagcaattcg acttcagttt ccaattccaa cccgagtgtc tctttggctt
    39481 tggctttgat gatttcaatc aacttataga catcatcagc ccgggcagtt ccatcatttt
    39541 cgataaaggc ggcatgtttt ggagaaatga cagcgccacc aatccgcaat cctttcaagt
    39601 tcagcacctt atctatcaag taacctactg aagaagtagg taggttaaac tgtttttggg
    39661 cttctggtga gaggttttta aaaatgcaac cggcggaacg acggggttgg aaggattttc
    39721 tacttttcca ctcttgagca acgattcgag cctgttcgac attgcccatc gtcagaatta
    39781 attccaccca taatacaact tctctggtct tctgcaaaac actaaaatca taatcgaact
    39841 gaaaataagc gttgtccact atttttggag agattccatc aatcaatttt gcgcgataaa
    39901 ccagatcgcc gaaataggtg tacgctccat gcatattcat ataaacagcg ccgcccacgc
    39961 tggacggtat tccgctaaac cattccaagc cggttaagcc ttgctcaaac atttcttgaa
    40021 ttaaggtatt attataaaat ccggagtcaa ctcggacgat tttttgtggt aaattctccg
    40081 cttgaatttc ttcttcttga gacgctgtca atggatcata tctcggaatg attcggctaa
    40141 atttaaggtt tgctggacaa tcatcaataa tttgccagga cttagaccgg ttgataatta
    40201 tcaaaccgtc atatccgtca tccgaaacca gtaaattact gccgcaacca ataattcgaa
    40261 atggtacctg aaaggcagtg gctaatttga tgaccgcttc gagtttttcg gtattttcga
    40321 caatcaccag taaacgtgca ttgccaccga ttccgaaggt ggtaaatttg gctaacggca
    40381 cttctttttg agcagtgata tgatttcggc ttagttcaga gacgaattct tctgatttct
    40441 ggatacttga tttatcttga ttagcgaaca cgtgctaaat taatactacc tgacgttaat
    40501 tattataaat ttttatcata attctgacaa atagccttta gtaaaaagat aaaattaaga
    40561 acctgacaaa ttaataggta atcgatcaaa aataattttt aaggtcatta ttttttgcgg
    40621 aaaaactttt actttcaaac gatagggatc ggatatttcc aacggggcaa taatatgttc
    40681 ttcgaccgta atgtaacggg cggctttgat tggaatataa gttgctattt cgccaacgat
    40741 tatgttgcgg gttggattat acaatctgag gactacatct ttctcatcct cagtgggttt
    40801 gagagccgaa taaaataatg tatccggggt aacctttaaa aactgcattt tcgccggtaa
    40861 gtatccattt gtttgatcca tctgtcgtat aattatcggg ttattgaaaa ttatagcctc
    40921 agttaaaata tcgctggatg tccaatcgga taatcgcgga taaatagata aatgaacctc
    40981 tgtcttttcg ttgcgtgata aaatagggcc gccggttttt tttgatcgaa caattggaaa
    41041 atcacggacc aaggttaatg tcaagacgtt atgcttgcct tttaatagtt ggtattcgtt
    41101 gatgccgtca ctaaatatgg caaatcctcc cgattcattg gaaactccaa caaaattgcc
    41161 catgctcagc gcttggcaat gaagattttg tgggctgtta aacggacgca tctcaaccga
    41221 aaaaaagccg tcagtataag aattctccgg tacaaactca atgggaaaac aaatttttat
    41281 catatggtca taggcatgat cattaatttc tatttttaaa tcaatcattc gcgaaaggct
    41341 acttaaagtt actacctcac taattttgat tgtctcatgc ctgggactgc gacgcgaatt
    41401 tttccaattg aacatttcgg gaatttccca aaaatattcg attttatagg cggcggcttt
    41461 aggagaatta taaagcaatt tgatttgagg atggagcttt tttgtagtaa ggggaatatg
    41521 agtcgcagtt ttatcctcaa cactgaatcg tcccagatca atttcatctt gaaaataggc
    41581 aacttccgag aaaaattcac cggtttcttt ggcaaatatt tccagcgttc cattctgatt
    41641 aatttcaact cgtaagaaat cattctccaa cgaattgcgc tcggcgctga tcggcacagc
    41701 cgagtataat ttcggcttgc ccttcggatc aatttcaaaa gttttccagc ccattccggg
    41761 caggtcgcgc aattctacta aacaacgata tttcgcttta tctgccttgt cgtactcaaa
    41821 tagtgtttca gtttccttat agagaactcg aaacggaatg tcacgtcccc ccacctcttt
    41881 agcaatgatt gtatctcgat taattaggga tgacaattcc gcgacaaatt caacaatttc
    41941 agatcggtta tatggcaaag gattaaatac cgtaaagtaa atattattag gattataatc
    42001 atcaatatga atattattaa caactgccgt aattaaatcc tttacctgct cttcgagctg
    42061 attcatcagt ccagtttgag tggtactaag tgactggata aatttattgg gattagtatc
    42121 tacgagataa gtaaatgtct ggttttttag gaattcctcc caaatatggt tggttgcctt
    42181 cggcctaatt ttcagctgta aatatttcat aattaatgac cacggttcaa caaaatattg
    42241 aattaggttc tcaagacgat gattacttaa accaatcaac acgctatgtc ttagataata
    42301 atcgaactga gtctcggtac aatcttcagt tttttctaaa cacatttccc ccttgtattc
    42361 acttatttca cgcgaatcaa tctgctcttt aatttcccaa atacaatcgg ccagagattc
    42421 aattttcaaa tcagaagaaa aaatcgaagc caatttttca atataagttg gcatggtttc
    42481 ccagcttttt ttatcattga acagataaac caagactcga ttgggattat ggctgaaata
    42541 ttcgcggaac gtctcctgaa ttttactttt tgctaaattt aaacgattag gatttaagaa
    42601 aaccacgcga ctgaccaata gttgggtacc atccaagcct tcccagataa actcggtggt
    42661 atttttatcc attaacttta tatcgtagtc aattaaaaca gcatcaatat tgaaaccagc
    42721 caaaatctgg ggcgtctgtg aattgcgtcc gcgcatcgct ggtaaatgaa agtattttaa
    42781 aatgtcaccg attttgtccc cttcctgatg gccaatgaga agattacgaa tcaacaattc
    42841 accggaaata tgtccggaat gaagttgaat gaaaaacggt ccgaaataaa tttttttctc
    42901 ttcgactaat tggcgaacta aatccgtttt ttccgggtga cttttcaaat attcgcacaa
    42961 agggtaaatc atttggtcaa aatgatacac ccctttctgc gttccttctt tcaggctatt
    43021 taaaagatta tcaatcaact cattgatttc cgcttgagaa ggaaaaggta atgaataata
    43081 gggattataa attggtatgc aatacaaact gttattaacg atcgacatta catctacttt
    43141 ttaatttcat acaagccgaa tttgcccaat ccaacttgtt ttctaaaata tatcacatat
    43201 ttagtgaaat tttcaattat tttttaaaat taatagatga agattggtat taatttatcg
    43261 gctgttaaaa atcggactgg attttttcaa ataattaacc tatttttcaa caatttgcaa
    43321 acagagccaa tatgaattta aaaaagaaaa tcagtatcgg cggaatgatc gaattaagag
    43381 acttgatgat ggtctcgatt ttctcattcc ccgatcaacc gggttcagcc ggaaaagttt
    43441 tgacttactt cgggcaaaga gggattagtg ttgattttat aaccgaaagc cgcaatttgg
    43501 aaggctcagc ggaccttatt ttctgtatca atcagaaata caaatcagac attacccaga
    43561 cgtttcagga actttctgcc attatcccaa ttaaggatac taaaataact gaaccagtag
    43621 cgattgtctc tttttatggc cctcatttcc gtgaaaaacc agctatcgcc gggacaattt
    43681 gtcaaacact tggcgaagct ggaatcaata tattaggaat ttcaaccagt atttcttcag
    43741 taagctgtgt aattaacgag catcaggttg acgccgctcg gcaagctata actgcgaatt
    43801 ttaacttacc ggagtagaaa tgaatcttcc tgattcattt actagtgacg aaagtaaaac
    43861 ccgcgaacag ttgctctggg aattacagca actacgtaat cggataaacc agcttgaagc
    43921 cgctgatctc gaacgccgcc gaatagaaaa atccctgaat aaatacgaaa agaattttcg
    43981 cgaattagtc aatctcctac cccaaactat ttatgaaatg gacaaaaatg gcaaatttcg
    44041 ctttaccaat caagtcggat ttgaaacttt cggatataat caacaggata ttgacaccgg
    44101 agttaatttc tcccagcttt ttattccgga agaacgggaa aaacttaacc gtaatattca
    44161 aaaaatttat aatggtagcg atctcgaggg acatgaatat actgccttgc ggaaagacgg
    44221 gacaactttc caagccctaa tctattccag tcctattttt tacgataaca aaccggtcgg
    44281 cttgcgcggt gtggtcattg atataaccga ccggaaaaaa gtcgaacaag atttgaagga
    44341 ttcgcgcgac cagtttcgta acctttcggc gcatttgcaa tcagtacgcg aagaggaacg
    44401 ctcttatatc gcccgcgaaa ttcacgacga actcggtcag gcactaacgg ctttgaaaat
    44461 ggacttgatc tggattaata aaagactaac taccgctcaa tccgaaattg ctgaaaaaat
    44521 cagcacaatg ttcgatctaa ttgataaaac aattcaaact attcggcgga tttcgaccga
    44581 cttgcgacct ggtttgctcg atgatttggg cttgaccgcc gctctcgaat ggtattgtga
    44641 ggattttcaa aatcggacag gtattcactg taacctacat ctgcgaccgg cggaaatcac
    44701 aattgatcag gaacgtagta ttgcgatttt ccgtatttta caagaaactc tgacgaatgt
    44761 cgctcgtcat gcccatgcca gcgaggtcaa agtcaacctc ggtattaaag atcacgcctt
    44821 agaaatgaaa attgttgata acggcatcgg aattactgat gagcaggtca acgatcccca
    44881 aaatctgggc ttggttggat tgcgcgaacg cgtttatccc tggaatggcc acatcgcaat
    44941 taaaggtcgc ccgcaaaaag ggacaaccgt tctggtcttt atcccattgg atggaaatat
    45001 ctaatgataa atttgctaat agttgacgat catcctttga tccgccaggg attacgccaa
    45061 accttccttg ataccaacga tattcgagtt agcggcgagg cgagcgatag tacttacgcc
    45121 ctcgctgaaa ttgaaaacca taactacgat gcggtaattc tcgatctttc attgcccggc
    45181 cgcagtggct tagatcttct caaacaaatc aaaaacctct caccttctct gccggtatta
    45241 attctcagcg ttttccctga aagtcaatat gctattcggg tattgaaagc cggcgccgat
    45301 ggttatatta gcaaagaaag tcatccagat gaactaatcc aagctgttcg taaaatcgta
    45361 accgggggta aatacatttc acccacgctt gcggaaaaat tggctaacag ccttaccgaa
    45421 accactcaaa atcccaccca cgaaattctt tcagaccgcg aatttcaaac aatgttagcg
    45481 atcgccagcg gcaaaaccgt caaggaaatc gccgctgaac tgaacctcag cgtcaaaact
    45541 atcagcactt atcgcactcg cattctacgc aaaatgcacc tgaaaaataa cgccgaaatt
    45601 atctgctatg ccgtccggct gggactagta cagtagagtt aattttataa gacaatgatt
    45661 attcattgcg attcgtagtc atttattggt tgaatgacac gaggtctgct actgcgggat
    45721 tattgattga cgttggtagt tattatttga tttgaaatcc caaatttcca attttccaat
    45781 tgcttttcag ctaaaaactc gctatattac ttacgagtaa cttacgagta tttgataaag
    45841 tgagaggaaa agcaaaatgg ctgaaaaact ttcgcccaag aaacagcgtt tcgtgaattt
    45901 cgttcacgac tttacggaaa ccaacaaacg tgccccgact ttcctcgaga ttatgcacgc
    45961 cttgcaaatc aaatcgccgg gtacagtcag ctggtacgtg cgcgaattag aaaaagccgg
    46021 tgttcttcgg cgttccggtc cccccaatgc caaacgtgcg ttgtccgttt ccgaaacgga
    46081 gagcgctagc actttgcccc tgctcggatt aattgcggct ggttatccgt tggaagctat
    46141 cgaaaaccag gagacaatcg ccgtgccacc agcctacatt cacccgtcta attacgtttt
    46201 gaaagtcaag ggcgattcga tgattgacga caatattcaa gacggcgatt atatcatcgt
    46261 tcgtcagcaa gctcaggctg aatccggtca aaccgtagta gcatttatca ataatgaagc
    46321 cactttaaaa cgctattatc ccaaagccgg tggtgtcgaa ctgcatccac gcaatccggc
    46381 ttatccggtt atcaaagtca aaccgggaga tgaattccga ctcggcggag tggtgcttgc
    46441 cgtgtggcgg gtgtacgaat gaataaaacc accaaggcgc taagacacga agatttttat
    46501 atacaatata tttgttcttc gtgccttcga atcttagtgg taaaaaaata aggagcaatc
    46561 atgtcaacaa aacaagttcc cttatcgcca agagaagaag aaattgctaa aaagattgta
    46621 agtgccgctt ttaatgttca ccaacatctt ggccccggtc tgctggaaaa aatatatgaa
    46681 acctgctttt gttacgaact tagcaagtat ggacttcatt accgtcaaca agttgagata
    46741 ccaattactt acgataacat agtatttgac gccggtctgc gcttagatat cttggtggaa
    46801 gatttggtaa tatgcgaact caaagcagtc acagaaatga atccggtatt tcaagcccaa
    46861 atattgagtt atttgaagat agccgaaaaa cgccttggtt ttctgattaa ttttaatgtt
    46921 cctttaatcc gtgacggtat tcagagaata attttatgaa gagaaacacc aagacactaa
    46981 gacacgaaga tgcttctaat tccattgatc caaaaatacc tgtccgataa aaataataaa
    47041 tattcctttg tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    47101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntaaaaata
    47161 ataaatattc ctttgtgcct tcgagcctta gtggtaaaaa atgtccgaat cggttttaca
    47221 tatccgtctc tctgattttg aactccaggt agagcgtatg ctggactcgg gtctgcgtac
    47281 ccgcgccatt gccgtgattt cctcgcatca tccaaacggt acgatcgtct gcgcctcacc
    47341 ggaagctctc agcgaaggca tttatcccgg cctgaaagtc accatcgctc gcaaaatgtc
    47401 gcacagcgtg gcactcctgc cctacaattc tactctttac actaaaatca atcggtacgt
    47461 ttaccaaact atcacgcgct attcgccagt catcgagcca accgtttacg gtcaatattt
    47521 cgccgatttg agcggcatgc atggcatcta tcgcgacagt ctgcaggctg gcaatctaat
    47581 tgcccgcgac attcaatcca aagtcagcct gcaaagtcac atcggcatca gcgttaataa
    47641 actcgtcagt caaatcacca ctgccgttgt cccagaaacc attcaccgcg tcgagccggg
    47701 caccgaacca aattttctgg cgccactgaa taccgaagtc ctgccggtaa caaacgagcc
    47761 gccggtcaat aaacttgtgc gcttcctttt cctttctcag gtacaaaacg tccagcaggt
    47821 aactactgaa agcgaaaccg gcgctattct cttcggcaaa tattaccgac gcctttttag
    47881 ggaagcccgc ggacaggata attctccagt cagaccaccg caaaaaaccg accgcatcac
    47941 cgaacaaacc gttctgaccg ccgacactaa cgacgaagtt caattgctgg ccgctgtaca
    48001 caacttagcc gaacagatcg cttatcaatt gcgtcggcgc cgccagattg ttagaacgct
    48061 gaatcttgaa attcactaca ccgacggatt caagagtaac cgcaaaggcg ccgtcatcgc
    48121 aaacgacgat accactgtca ttgcggaagc tagtcggcta ttctcattag ccaattaccg
    48181 ccgcaaccgc gtccgagcca ttattctgga tgcaaccgac ttccagccgg tgattaatca
    48241 attggactta tttcgaccac ctaaagatga tcgtctgtca caggctctcg accaaattcg
    48301 ccagaaatac ggctttgcca gtattggcag tgccgataat cttctgattc ccttaaataa
    48361 aaccacaaag gctctaagac acaaagatat ttttggtgcc gttatttcaa ataaaaacgt
    48421 ttcctgttct gataaaatag cattaccggc ttaaaatgaa ttaccaattc cataaaaaac
    48481 taattactac ccaactcata ataactcttt gtgcatttgc gccttcgtgg ttcactatgt
    48541 gcatcaatcc taaaaactct aagacacgaa gattaattat gtacaaaata ttatccttag
    48601 cgtcttcgag tcttagtggt aaaaagaaaa taataaatga gttctttgtg cctttgtgtc
    48661 ttagtggtaa aaataaaaca ataaatcttc gtgcacttgc accttagtgg ttcattacca
    48721 tgttcgttca ccttaatact cactccactt attcgaagat gcgcgggacg gcgtcgccgc
    48781 aggctttact gacgcaagcc aaagccgctc accagacaca tttggctttg acggaagtca
    48841 acggtctgtg gggctttatt cgctttgtgc agtgggcgcg tgaagtcggt atccagccaa
    48901 tcgccgctac caatttgctt actccgaatt tcgatctcat cctgctggtc gaaaatcagt
    48961 atggttacga aaacctttgt cgcgctattt cacgggtaca cgataatgaa aatctatcca
    49021 tagtcgaact cctgaagccg tatgccaagg gacttttcgt cctggcatat caggaagacg
    49081 ttctgtctga actcgcgtgt ttcattcctg ctacccatct tttcgtagaa ctgcgtcccg
    49141 gcgtgagcga acaagaaact cggcgcctcg cggaaacctt tcatctcgaa atcgtcgcta
    49201 ccggcgatgt ttattttctc aaaccagaag atcagaagac tcatcgcatt ctgcgcgcca
    49261 ttgataaaaa ttccacgctg gcacaactcg cacccagcga ttacaaaagc gaacagcatt
    49321 tctttcgcac cgagaaggaa atgattgacc tctttcccaa tagcctgaat gctatcaaca
    49381 actcgcagtt tctggccgaa cgctgtaaaa ctgactggaa ttacactaat acgattttcc
    49441 ccggtctttc acttaaggaa accaatctcg ccaatcgcca actacgccaa ttggtttacg
    49501 atggggctaa aatccgctac ggcaaaattg atcaggttgt caaaaagcgc attgagtatg
    49561 aactctctct gataacccag aaaggctacg cgccgtattt tctcattgtc tatgacatcg
    49621 tccagcagac tccaaccact atcgggcgcg gtagcggagc ggcttccatt atcagttatt
    49681 gccttttcat tacgcaggtc gatcctatca aatacaattt gtgcttcgaa cgctttattc
    49741 atcccgaacg gattgatatg ccggatattg atattgattt cgcctgggat gagcgcgacg
    49801 ctattctgga ttacattttc aaaaaatacg gactcgaacg caccgctatg gtttccaacc
    49861 aggttatgct ccagccgcgc tcggctattc gagaagtcgc caaagtctat ggcctatcta
    49921 atgaggaaat caaagccgtc accaaacgcg tcggctactt tgccagaaaa accgaactat
    49981 tagaatggct cagcaccgat ccgcgttttc gcaatctcga cctcgatgac actttgcgcg
    50041 aaattatcct gcaaagtgag aaaatcgttg gcgtctttca ggtttcatcg gtacaccccg
    50101 gtggtgtaat catcgtaccg gacgaaatcc gccaatacgc gccagtgtta acggctacca
    50161 aaggcgttca gatcgttgaa tgggaaaaag atcaagtcga ggattccggc ttgctgaaga
    50221 ttgacatcct cggcaatcgt tcgctggcgg ttgtgcgcga tactttgaaa cagatcggtt
    50281 tgtaccgcag tgaatttatg gactatcata aaattcaacc ggttggcgat tcggctacgc
    50341 aggatttgat gcaaagcggc cgcacaatgg gcatttttta tatcgagagt cctgccacgc
    50401 gcttgttatt aaaaaaggct ggacgagttg atttcgagca cgtcgtgatt tactcctcaa
    50461 tcattcgtcc cgccgctaat cgcttcatta atatcatgct ggcgcgaatt catggccaac
    50521 cctggcaact cttacatccc gacttgaaat gtttagagga aagctacggc attatggttt
    50581 acgaagagca ggtttcaatg gtagcgcgcg aaatcgccgg ctttagctat gctgaatcag
    50641 attacattcg caaagtgatt tcgcgcccgg cactggcgca tctagttcct atctggaaaa
    50701 agaaattcat caacggcgca ctggcacgcg gttattcccc aaaattagcg gaaacgctct
    50761 gggaaatgat cgaatccttc tcgggttatt cattctgcaa gccgcactcg gcctcgtatg
    50821 ctatgctctc gttcacctgt gcctatctga aagcccattt cccggcggaa tttctggcgg
    50881 cggtaatttc caaccaaggc ggctattatt caacttacgc ctatctgagt gaagcccgcc
    50941 gctggggcat tcaaatcctg ccaccgcata tcaaccgtag ttggcaagaa tatcgcggac
    51001 ggcgtaacaa aatccggatg ggttttatgg cgatccgcaa cctacaggac aaagccgtcg
    51061 aagcaattct caaggaacgt aaaaacggcg atttcaaaaa cctcgatgat tttctcaaac
    51121 gagttgacct tgacctggcc gacgctatgg cactgaccaa tgctggttgt tttgctgaat
    51181 tcgagccaca attatcccac cgcgaaattg ccttcaaggt cgccagctat tacttacaaa
    51241 aaggcgaccg ccgaccgctt ccgcctccaa ttgaccgtca acctatctct cggttggaaa
    51301 aacttaccct cgaaatcgaa tcgttcggat ttccaattgc ggaacatccg ctggaacgct
    51361 atctaccggt tttcgcaggc aaagtcaaaa aagcccgcga cctggcgcaa tatgtagggc
    51421 aaacaattaa cctgctgggc gtttacatta cgcgtaaagt cgctctaacc aaacagcatg
    51481 acccgctgga atttgtcact ttcgaggatg aaaccgatat tttcgagtgc gttatgtttc
    51541 cggaatcata caaggaattc ggcgacctac tgaactggga aacgcttttc gttatccgcg
    51601 gcaaggtcga ggaagcttat cagacttaca ctgtcacgat cgagaaactc gccagcctgc
    51661 aacgcaccgt cgagaaaatg tcgtcaggac ggcgaatgct tgtcaaaacg agttccgcag
    51721 aaacacattt tgctaaggaa tattaagaat acagatcacg cttattaatt ttaccctttg
    51781 tctttgtcaa tataatactt tcggtatttc ttccttccat ttgtttgtag ctcgccgtgt
    51841 ttctgtggca ttgtttatag aaatttattc aaattaccct ctttccattg ttttggattg
    51901 ttaatgatat attgccgtgt tgtggttgat ttgaataatc gtgcaatggt tggaattgaa
    51961 ttttttgttg gtttcccaaa ttgttcaatt gtccccgtat ccgtttccgt ccccatttcc
    52021 gtatccgtaa ttgtatcggt atccgtaatt gtattggtat ccgtaatcgt aattgtaatg
    52081 gtaatggtcg tagagacacg ttgcaacgtg tctctacgat taccaccacc acggaaacca
    52141 cgaccatcat catcattatc attaataatt aaaatcccgt gtaaatgatt tggcataaca
    52201 acgaatgcgt caataacaat attcgggcgt ataatgccgg ttttcaacca ttcattttgt
    52261 gccaattccc cgaattcgtt ccacatcatt tcaccatcaa caatattccc aaataaatat
    52321 tgccggttgt gcgtaacaat tgtaataaaa tatgcgccca gtcgggaata atcgtacccc
    52381 cggcaaacga acaggttaaa tacgatattt gtctttgaat agggtcattt tttattttct
    52441 attatcctga tttcttctat ggtatagtga gtttatttca actataaacg taaatttcga
    52501 tatcaaggta tgaaagctcc tctggaaata cttaatgagc tgaaaccatc aaaaattatt
    52561 ctttaaaatt atctctaagg atggatacca tgtccctaaa tcagatcatt ttatttttaa
    52621 accattattg ataagattaa attaacgccg ctatttggag aggtggcaga gtggttgaat
    52681 gcggcggcct cgaaagtcgt ttcccgatta
//
LOCUS       DAOSMP010000013        49945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_745_length_49945_cov_19.011044, whole genome shotgun sequence.
ACCESSION   DAOSMP010000013 DAOSMP010000000
VERSION     DAOSMP010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 49945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49945
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_745_length_49945_cov_19.011044"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PKX09_03765"
     CDS             complement(<1..269)
                     /locus_tag="PKX09_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018034441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOU16970.1"
                     /translation="MNWFKKDIEKKILALLTLLLSFIVYLDTISPTVPFWDCGEFIAT
                     AYTLGVPHPPGSPLFLLIGRIFSMIPFSQDIAFRVNLISTIVSALA"
     gene            complement(296..607)
                     /locus_tag="PKX09_03770"
     CDS             complement(296..607)
                     /locus_tag="PKX09_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOU16971.1"
                     /translation="MPEKQPDRQQILFLSLVQSLVSSAWIHLGKMKNPVTGTNSIELE
                     EASMSIDMLEMLHNRTQGNLSDDEKRFLENALSDLKINFVEAKLHQSAASEKPNEESH
                     S"
     gene            complement(616..>2013)
                     /locus_tag="PKX09_03775"
     CDS             complement(616..>2013)
                     /locus_tag="PKX09_03775"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOU16972.1"
                     /translation="QLLLAVRRAGIKAIQKPQRYKSAGPLLKQIYAKLPFELTNAQKK
                     VVREIWNDMHSPTLMNRLLQGDVGSGKTVVALLCASIAIGNGFQAAVMAPTEILADQH
                     YRVLKQLGEVAGIQVRLLIGGQKKQERQTILDEIQAGQAELIVGTHALIQENVSFKNL
                     ALVIIDEQHRFGVVQRSTLISKGYHPDVLVMTATPIPRTLSMTLYGDMDISVLDEMPA
                     GKGRIITRAVTADQIGKVYDYIAKQLPKGNQAYVVYPLIEESAKTDLQAAIQGYEYLS
                     EKVFPQFRLALLHGGMSIAEKDRIMQDFIQGKIDLLVSTTVIEVGVDNPNATIMLVEN
                     AERFGLTQMHQLRGRIGRGRKNGVCILVERKVTELGHQRIKTILSTIDGFEISEADLK
                     LRGPGEFYGTRQHGYLKMKIADPLTDIELLKTARVRAFTLLKSDPHLRRGENAATRNH
                     LLQQYANQIDFSNIL"
     assembly_gap    2014..2113
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2114..2478)
                     /locus_tag="PKX09_03780"
     CDS             complement(<2114..2478)
                     /locus_tag="PKX09_03780"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecG"
                     /protein_id="HOU16973.1"
                     /translation="MVNERPSSKKTVDLRPETPITYVKGVGPKRAEVLAKVGINTLKD
                     LLYYFPRRYLDRSTITEIAQLKVGMDATVVGKIISQNLVRRYRRPFFQVEISDGSGIM
                     TAVWFNGINYIKNAFKVGET"
     gene            complement(2472..3851)
                     /locus_tag="PKX09_03785"
     CDS             complement(2472..3851)
                     /locus_tag="PKX09_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16974.1"
                     /translation="MKKLKNGILLATVILGLIRMVFADTPVKSTQITVYNNNLGLVLQ
                     TRTLNIAKGDSEIRIEGVPAKVDPTSVRITFPGKADAIEVLEQNFLYDLVNSIKIFEK
                     YIGESISYKTKSGEQTNGKLLQFDGGSLILQLPNGGIKIASAENISEYDFPSLPSGLI
                     LKPTLQWLLSSRINAAVESEISYLTAGMNWHAEYLMVLDKNDRDFTLNSWVSLDNQSG
                     ATYENAKMKLVAGDIHRAPQPAPVRREKVYAADVLTQVAPFEERGLFEYHLYELQRPV
                     TIKDREIKQITLFDEVNAKAEKFYLFKNSAFSETEGPLEVHLKFANSTANHLGIPLPE
                     GVFRIFKKDVDNTLQLVGEDRIAHTAKDDTLDLIVGKAFDVKGQKTITDRQKISDRSE
                     IISIKLEISNKRTEPINVEIQEIMSGDWFVKNASHPYQKKSNSLLVFPLTVPADKNII
                     IKYSFQRSW"
     gene            complement(3896..6559)
                     /locus_tag="PKX09_03790"
     CDS             complement(3896..6559)
                     /locus_tag="PKX09_03790"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOU16975.1"
                     /translation="MSIQLDKVYDPRQVETKWYDYWLSKNYFHAKADSPKKPFVIVIP
                     PPNVTGILTIGHVLNNTMQDILIRHARMQGFETLWLPGTDHAGIATQNVVEKNLRKAG
                     KTRYDYGREEFTKLVWDWALKHKDIILTQLRRIGCSLDWERERFTLDEGLSRAVRQVF
                     VELYHKGLIYRGRRIINWCPASQTALSDEEVVHRDTKGHLWYFRYPLADNSGFITVAT
                     TRPETMLGDTAVAVNPADSRYQKLVGRNVKLPIIGRIIPIIADEFVDREFGTGAVKVT
                     PAHDPNDFEIAERHNLPKINILNPDATMNEAAGPLVAGKDRFEARQLVVAEMERLGLL
                     EKIEEYSHSVGYSERAGVMIEPYLSEQWFVRMQPLAEPALKVVEEKKIRFYPEKWVKV
                     YKHWMTNIRDWCISRQLWWGHRIPVFYCDACGWSDALMEDPSACPKCGGAVRQDEDVL
                     DTWFSSWLWPFSTLGWPDDTPDLRKFYPTDDLVTGPDIIFFWVARMIMAGLEFCHEIP
                     FRNVYFTGIIRDAQRRKMSKSLGNSPDPLVLVEKYGADALRFGIMLIAPQGQDILFSE
                     ERLEVGRNFMNKVWNAARFVLMNLDENTLSGEPFEPKRMQLELSDEWIISRLNDTIRS
                     VDKNLKKYRFDTVARTIYDFVWSDYCDWYIELIKDRLYKKSLAEKQTALRVAVYVLKN
                     FLKLLHPYAPFITEEIYQHIKLPNEPDIVVSTWPKVEYHPKDKALTERFELLQEIVTA
                     LRTARSEMNISPAVSIELIVRSARSSPVTELLNNSEIQNYIKNLVKVTQITFVAENVK
                     PAHSSTVVVRGTEFFIPLEGVIDIAAETARLTKEINRLTGILKGASAKLSNRDFLARA
                     PQEIINKERAKEQVLKEQLDKFKANLKLLNN"
     gene            7092..8003
                     /locus_tag="PKX09_03795"
     CDS             7092..8003
                     /locus_tag="PKX09_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16976.1"
                     /translation="MKKKSKKYNPLQMEKELSDLIEYIENAGFESKEELDRFLAKMGG
                     KKIGSVLNKKKTAADEAFELILEAQEVSPEEGIKLAKKAITLDPNCADAYTFIADHTE
                     DEDEALELYQKAMQVARESLGENAFIEYVGHFWGITKTRPYMRAKAGYASSLILKGDL
                     QSAIKEFQEMLVLNPSDNQGIRFSLSVLLLQLNRLREYEDLLKQYKDENSAGWLYNRV
                     LYLYLKYGGTTRTISALEEAIAENKYVMDLITGLEEPPKELPESYGIGSYEEAVLYYW
                     ESILTWMAHKKAILWLIRYWERRREIN"
     gene            complement(9280..9930)
                     /gene="phoU"
                     /locus_tag="PKX09_03800"
     CDS             complement(9280..9930)
                     /gene="phoU"
                     /locus_tag="PKX09_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOU16977.1"
                     /translation="MLTKKIQELKQAIVEYAALVESMITRSLQALSAKDVNTLKEIIN
                     VDESKANEFDLRMDEACIQTIAQYQPRARDLRTIIMILKMSNDLERLGDGAVHICRDA
                     LYLIDRPSFKPIPLLNHLAQESLGMLHDSIDAFINNDAESAQEICKRDDVVDDLRDKI
                     IAEMTLLMSKQPATVPIALRVMDISRKLERAADLSTNICEDIIFIVQGRVIKHGKN"
     gene            complement(9954..10721)
                     /gene="pstB"
                     /locus_tag="PKX09_03805"
     CDS             complement(9954..10721)
                     /gene="pstB"
                     /locus_tag="PKX09_03805"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOU16978.1"
                     /translation="MNLSKEIRIQAENVNFYYGDKQTLKNVNMSIFQNRVTAIIGPSG
                     CGKSTFIRLLNRMNDLIPNTRVEGRILLDNHNIYDPGVDVVTLRRKVGMVFQKPNPFP
                     KSIRDNLSYGLKINGIKSTDSIENTIRRSLEEAALWDEVKDRLNESALRLSGGQQQRL
                     CIARCLAVEPEVILFDEPCASLDPISTKRIEELIEQLKQRYTIVIVTHNMQQAARVSD
                     YTAFMYLGELIEFGPTEKIFTIPENKHTEDYISGKFG"
     gene            complement(10708..11562)
                     /gene="pstA"
                     /locus_tag="PKX09_03810"
     CDS             complement(10708..11562)
                     /gene="pstA"
                     /locus_tag="PKX09_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOU16979.1"
                     /translation="MKRNSSIQYFGFGFLYLAIFIAVLTLGAIIYFITVNGIKVINWE
                     FLTQVPRRAMTKGGIAPALVGTFYLTIGAIIFALPMGLACAIYLTEYSPKSVVINVIR
                     MSINNLAGVPSVVFGLFGLTVFVKFFGFGVSILSGSLTLGIMILPGIISAAQEALIAV
                     PQSLREASLALGATQWQTIRKIVLPSAMPGILTGVILSVGRAAGETAPIMFTAVTFYT
                     RGFPNSIMDEVMALPYHIYALMTEGTFPERQTMIAYGCALILLFLVLIVSGLAIYLRQ
                     KQRKYELV"
     gene            complement(11559..12443)
                     /gene="pstC"
                     /locus_tag="PKX09_03815"
     CDS             complement(11559..12443)
                     /gene="pstC"
                     /locus_tag="PKX09_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOU16980.1"
                     /translation="MKWNSEKVVKPVFGGLAFASLVFLLGIILILFQQGLPIFRIVNL
                     FEFIGGKAWYPTAEPAEFGILPLILGTLWITLGAMLFCIPVGIGSALFLHEIAGFRLR
                     SILKPIIEILAGIPSIVYGFFGMVIIAPFLQNILDIPIGQCALTASLILGVMAVPTVC
                     SLAEDALNFVPKSFREASYALGANRWQTLTRVIIPAAGSGISTAIILGMSRVVGETMT
                     VLMVAGGAAVIPKSFLDPVRPMTATIAAEMGEAVVGSPHYHALFAIGLVLFLITMAFN
                     IIAEIISRNYRLKLGLGR"
     gene            complement(12525..13337)
                     /locus_tag="PKX09_03820"
     CDS             complement(12525..13337)
                     /locus_tag="PKX09_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU16981.1"
                     /translation="MKKLFNLSILILAIASLLTAGETVKIRIEGSTTVLPIAQLAAEE
                     FMIANPDVTISVQGGGSGVGIASLIDKTCDIATSSRDMKDEEIKNAVAKGVNPVAHIV
                     AMDGIAVILHPSNKINNLTVEQIRKIYTGKISNWKEVGGDDKKIVVLSRDTASGTFEA
                     FSKLALNDEKVRQDALMNASNQAIATTVEKTPGAIGYVGIGYISSKIKAVTVENVTCT
                     KKNILTKTYPLSRPLFMYTNGKPKGVVKDFIDFILSEEGQELVETAGYVGLK"
     gene            complement(13356..14594)
                     /locus_tag="PKX09_03825"
     CDS             complement(13356..14594)
                     /locus_tag="PKX09_03825"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HOU16982.1"
                     /translation="MKLRERKVNIILALLGFCALTLNAGELDRLVNELVEKGVLDAGK
                     AQEILTLTEEEMRVEMNAGNSTTLPKWIQNMGLKGDFRFRNQWEATEEDSVSRMRQRI
                     RFRLNGEAVVTSGFKVGFGLATGSSDPRSTNQTLDNSFETKPIMLDYAYGSYTFPKYA
                     NVLLGKFYSNLGFWTPTDYMWDTDITVEGVAAQFNHKMIFFNTGIYFLDEKGKVENVI
                     ADNPRMLILQPGFNLAKEGIYSLKAAATYYNFNKTKNMAFDHAKWSNSYITINDSKYL
                     ALGYNNFNFATEVALYNKIFPYIGVCFEINQNLQAESDTAKNGIACGLTLGSEKIKDK
                     GQWQVKYVYRQLEKDAWLDFLPDSDTYGGKTDIKGSELIAQYGLNKNIVLALDYYKIE
                     NLHGAIKPETVLQTDIQFKF"
     gene            complement(14931..15473)
                     /locus_tag="PKX09_03830"
     CDS             complement(14931..15473)
                     /locus_tag="PKX09_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HOU16983.1"
                     /translation="MKVQLKKEIEQIVHLQDYAGKPTIEGVKVINLRRHNDDGGSFTE
                     LARITNGDFSGVEEFKIAQINFSEMEPQSVKAFHVHRQQTDIWFVPPDSKILLILIDL
                     REDSATNGQRMRLVIGDDNSQLIVIPPGVAHGCKNLGLKKAHLIYFMNKQFSPEPSEC
                     DEWRLPWDYFGGEIWEVNKG"
     gene            15764..16135
                     /locus_tag="PKX09_03835"
     CDS             15764..16135
                     /locus_tag="PKX09_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16984.1"
                     /translation="MAITVFIISLFIGIFVLNLLILRYLKGSIHKSSLSVRLPKTVAA
                     FDRYTLKVVALVTLIIIIFMLIAIALQSGFVIPVIQPIALILFIFITGVGFVYFFKSE
                     RNISKSDQKFSTGETDGEEQK"
     gene            16132..17172
                     /locus_tag="PKX09_03840"
     CDS             16132..17172
                     /locus_tag="PKX09_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16985.1"
                     /translation="MKSENVGNWQPLRFIFPDNTAISFDARIADSVIKELALDTGQPV
                     FKLRCSINELYQPNTQIFTSGFPYNDLWFRYAQILVIEKHLKLQAPVGIELIRVIFCE
                     FLRIAEYIFNLATLCLNVGLNDIYSGLMQQYESIQQYFEQILSGTNPMDFIIGHKTPE
                     DLTVGFTEMTLNLSHQLQIQLRKSLPHLLNNRLILSRFSKIADQTADNSAILNDKLEF
                     DQMRFTSSKKQNNVCWQLLKIRCDDLFRSIGLIRLSLTKFVYLDRSPQTLPSLTSLPK
                     GNSSVNIETPWGKLSCQMNCPGDRNPCDFILSNPLHKIQLLLCKMAVGLELDELPLLI
                     TSLGLRPKIEPR"
     gene            17399..18181
                     /locus_tag="PKX09_03845"
     CDS             17399..18181
                     /locus_tag="PKX09_03845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOU16986.1"
                     /translation="MTTQVFLWIETGYLMALWLVLPYQMQIQGNQTLWLFLAIITLPL
                     NIINSIITSEEAKSEISQAGIFKNGISLISSFAPLLLSILILITGNRYTSFWEISSQQ
                     SGYRLGFIPNWTIFSSPLFLLIGIIFLINLTMLMRLSDGSYKPLEYEELAEIYSGRYE
                     SFGILPQFNRSAMLFTLTTLAIFLFFGGWQTPLEPEGQNLASLAGGLIFFVKTILIFS
                     ACLAIKYHLPILTNWQIIKINLRIQIPVLTLIWLGLLIIRWL"
     gene            18181..18669
                     /locus_tag="PKX09_03850"
     CDS             18181..18669
                     /locus_tag="PKX09_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16987.1"
                     /translation="MTNFQWMPVIFWLATFLTLLPAGLAITSTNRKSLILSNMFTQLG
                     VFLLIISLGVGSPAVIFLVLVFPLNIMMLYGHNLFPETNKQTARESRPAIFLKTAVCF
                     LLFLLLIIAFYQNHLKNMLVIDNSGKILEGVIPDVSLLLILIGMFIMLILVAISHFLR
                     RK"
     gene            18666..18971
                     /locus_tag="PKX09_03855"
     CDS             18666..18971
                     /locus_tag="PKX09_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16988.1"
                     /translation="MSHLFLYQIVGIGLFIIGLYGLWRNRHCWAVITFLQLMSSGILL
                     SFLAIIRTKGAYQSVSELIVIFIFAVLIIEILLAIVFTYKVLMSDSSSNSDDISNRG"
     gene            18973..20862
                     /locus_tag="PKX09_03860"
     CDS             18973..20862
                     /locus_tag="PKX09_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16989.1"
                     /translation="MNILIILLLLFPVCAYLLTRCLPDKYLLNRRWLSILFLVISGLI
                     GLGIFIVLLLINMPDYVQRISWTWANIAGHSLQISLLLNNFSGLLLALWALGSLIFAI
                     LTATDFYSPDHFRENVTNLALGTLAVNGLILANDFLSLIFFWALIDLSIYRVIFRKFD
                     KASESYLPHIMVIGGHILLILAAFSFYKICGTFRFDLIIEEVLSGKVDPQQLILPGSM
                     LILVGILRIYSPFFRYQFNSQTKMPISDEFILFCGLMPSGLFLVLKLFPIMPQIFLTG
                     LMIFSGISVLIFLIIHLAKTGLNQFLHLNTIFLISLLIITIGLGTFPISASLLFILFM
                     TNFMLIILAQDFQPMSDSANFSIALPFRLIAWLSLIGLPISLAFNTRLLLLQASLTLS
                     SNKPIHGTIISALLVLNYFLNVVVVFRIFLKSGWYEPKHRKIDRVTPLTHCIIAGISI
                     VCLYLFFTLPNFNPLTTELRFFPLFSIQGSQPLDYSQYSSNSLIIYSAILLIGLVVGF
                     ITAKYFKAEKFSRWLTEFSLDFRRYSIIPVANFSQVIAQKIRYFDTVLFSRVSQSLMQ
                     TARTVILFINRINGIGAQQITKLKEVPEKYLNRNLDGIFNLKFQVLIGIVLSILIITV
                     LICIL"
     gene            20887..22365
                     /locus_tag="PKX09_03865"
     CDS             20887..22365
                     /locus_tag="PKX09_03865"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOU16990.1"
                     /translation="MTEHLLSINLLIALIGLLGIALTKREKGNLIKTIAAIVTGLQFW
                     TIIEILFNFNPMDTALQFSERINWIPAFRIDYYLGVDGINLVFLIVTSLLIFLSVIIS
                     WNKGEPLKEYFILLMILDIGLNGIFIAINLFLFLIFLGVTFFAIYLIIGFSNIRGSVN
                     SVNRFGIPFLISYIFILLGVLLLYKSNNLHTLNLTELTVGNAISHSKQMVISIILLIG
                     FAILIPLFPFHSWLILLIKNGPKEVSLLILGIVTKVGLYGLVRIIAPVMPFATNSLTL
                     ALGIWGLVNIIYGAICALGSDDSNQILGYFTLQQLGFFLIGFATLGENSNISVAVTGL
                     SGSLLIVISHSCLATLLLIIFKNIQAENQNVARSSLPNIAIVTLTLLAGIGMPGFLDF
                     FARFLILLSAFQTQRLLILAIISLLGIFLNFAIYMKLFKKLVFSQHTRDNAKRLDSNE
                     VFASVSLLIVLILCGIFPSFLLNLIQPGITRLLEMFSMAIAF"
     gene            22373..23776
                     /locus_tag="PKX09_03870"
     CDS             22373..23776
                     /locus_tag="PKX09_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOU16991.1"
                     /translation="MSNFQSIIYFIPEIIIIIAAIGFLLLTTFFKISDSKKYFLISVS
                     LIISLIATLLSHSADGHSIFNNLLVWDSYGQFIKIFTLIIACIILIIRRKENCNEASP
                     SPVTEFGIGLLLLISVAFVSINANHLVALFISLQILHLAGFFIVQSRNLSPRIITASW
                     HDLMFDIFTSLIMLMGIALIYDTTGTFFIAEIGQILSTVTRMNFPLILGIALLLVGFS
                     FTITGIYRLSWKLEILRTISASDRLLLLTIPQFIETTTLIRLLRVGLGLVGDWRPLTL
                     MIGIFAVLTLLFPIIWLIKLRSAKYIIIVTSIAQSGFGLLALCAGNISGDVAALFYEV
                     VLIFSLIAFTIGEILTINAKSSQLIALASLIGIPLTAGFPAKYLVFISLVQHNPFYYF
                     LIVIGIGSAALMLIGYVRIVRQIITAQGQFFNSPQKGSQLIFFIQILINIILISIGFY
                     WTPLTDYLQSIMTFCVP"
     gene            23865..25394
                     /locus_tag="PKX09_03875"
     CDS             23865..25394
                     /locus_tag="PKX09_03875"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOU16992.1"
                     /translation="MSPQNKRFFFWFVIAFLIIFIILISISLIHNFNKSKIISLPAQE
                     MRGVWMSRFDYTEPFPSYNPDSMKTYISDSFRRLKDANFNVIFFQVRGNADAFYASRY
                     EPWSNLLTGELGQNPGWDPLAYAIETAHQLGLELHAWLNVFPAWRGTAPPTTSQPLHP
                     YLAHPDWLICDGNGKKMLLSDGYVSFSPGIPAVHNHLIKVISDIVSRYDIDGIHFDYL
                     RYPEQSELKGYSHDPISWARFQSHEGNPLGLDWTDWQREQINEFVARAYNCVTDIKPE
                     IKVSAAVIGSYKTDHWNGYSAGFQDPRRWLEIGKIDLIIPMTYYHLNHKNFTFNKALK
                     EWQSVIDNPDQIFPALAAYNLSWEEIIEEIHLVRNSHFKGMVFFAASSLDSEKLQSLQ
                     TTEFQYPSIFPCLTWKDSVAPPSPINFAVEKLTSDSLEFIWQIPHQSNDIKKFVIYQS
                     KNLPIDLTKGENILAIVAGSDTSYQTKFNLPDQRRYYYTITSLDAAYNQSEPASVIKI
                     K"
     gene            25611..26561
                     /locus_tag="PKX09_03880"
     CDS             25611..26561
                     /locus_tag="PKX09_03880"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU16993.1"
                     /translation="MKVKFWGVRGSIPTPPTSEQIRHKFLRIIDNLPTNIRKNPTEIK
                     KYILSLSYLEAGLIGGNTSCVEIRADNKILIFDMGSGLRALGNYLVKNEQNKNGLDLH
                     IFISHTHWDHIMGFPYFTPAFFPNNKITFYSPHPDIKERLEIQQDSRFFPVSLEHMLA
                     KKEFIQLQPKTSITLGKVKISNYPLYHPGGSYGYRINWDGKSVVYATDSEYKNLDRES
                     TKHYVDFFRGANLLIFDAQYTFEEAIHKEDWGHSSALIAVEFAVEAGVKKLALFHHEP
                     ERDDFEINDILKKSRDFKKINFPNEILDIFLAMEELEITL"
     gene            complement(26833..>28227)
                     /locus_tag="PKX09_03885"
     CDS             complement(26833..>28227)
                     /locus_tag="PKX09_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16994.1"
                     /translation="PLGALSNIFSNVLITAPILIIYFGLYIFGIKLHILWLLLALCLS
                     GALTEILCFKFFSISPFRININKNSCTTCGNCDDNCPQGIPVSSYEKVTHPDCTLCLD
                     CTKECSSSGSIRLNKGNYGWLPPVALMVLFILALLTARNFEFKTLSERWGNYAELESV
                     QKFEMEGLKSVKCWGSSKSLQNKLMKQPGIVGLDTYVKSHKVVVYYDSTKIDEYGIKK
                     AIFTPYQYKIQKFDEYQPEKIAVFEIPISGLWDVQDNADLIRMLRVNPKIVGFETNFG
                     EPVIARIYVELGKVTPDSIIYLINQKSYEKKLPNGKTEVVKVNFRCEGRGAFVDTLDY
                     VTFRRQLFAGYDQAFNSYEKYRPADLMIFEIGLPDAESIAVRRALKYLTSHISFFDGV
                     VRVRTDYTDREALLIYFDPTQVNPKDIQTKLNEPMLKILTSDEKIMEMENSFEFREPL
                     RIYQVTPEMYALEK"
     assembly_gap    28228..28327
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28328..28949)
                     /locus_tag="PKX09_03890"
     CDS             complement(<28328..28949)
                     /locus_tag="PKX09_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU16995.1"
                     /translation="MRRNFFHNKTKLLFQAIILLLIIILVIIGCTDKAKKADFEAYCP
                     FGGLVSLGSKFHLGSMSCSMNETQVFMGIFLLIGVILIGKLFCGYICPIGTVTEWLNK
                     LFARFKFSIVLKGKADRILRLGKYVLLFFTAYFTATASELWCKKFDPYYAAVTGFGSD
                     TVLLVGILTIFTVVVLSVFIRFFWCKYICPLGALSNIFSNVLITAPI"
     gene            complement(29066..32728)
                     /locus_tag="PKX09_03895"
     CDS             complement(29066..32728)
                     /locus_tag="PKX09_03895"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HOU16996.1"
                     /translation="MKKVLKVTALMLTVANLLLGGSWQTVKIASTDMSVNKVSFIDAN
                     TGWIVYSTTYPSKQMGKVQKTTDGGANWTTVREPDVSANSWNDMEFIDANIGYACGNG
                     GIIYKTTNGGLDWTMIGDTNTYKFDLYSLDVVNANVVYCAGKSGTVLKTTNGSTFNKL
                     SYNFDGDMTAIDETRDDLDGGIAFFDENRGVVAVDFTKEATIWWTEDGGTNWNPVKIA
                     AAFPTGATSYRVYDVAVGGDSTVALACYHRVCLVSTNGGRTFENKAVVQFANVYTSYV
                     EVFDNNTIIYGGGSDNFGAITTNGGTDWTNFSTGTGQRLLSADFIDARTGYWFCEYQQ
                     WMKTADGGVNWIPINNWPGISWLGLAFPDEKKIVLTADGGGEMTFSNDWGKTWSYPNN
                     LRTGLDGDIFECEFIDANNGLIGGSGGYILKTTDGGATFTQIDNPMYNNNKAVNAMHY
                     LNSDTVFVGGGSGYICYSFDGGNTWTKATAGSKTVYDIWPLSNNAVVATCASGEYYTS
                     TDLMTFTKLGATSEAKNLRAVEFRNGIGIIPGASGYIYRSTAWDAMPTLVYTASNGAE
                     LYDVEFVDDNTVFVVGQYGTILKSEDAGLNWTVETSPTDEVLQKVRYSDNGSVGILWA
                     VGQNGTILKYVIYSNVLAGDYYIPQGDHEQGFATLGAAVTALNTYGAIAPVTFYIDAD
                     LIESGSELIIDREDLTAETALTIKPASGKTPSITINDFPTTGNYSGQGFTIENTAYVT
                     IDGSNTSGGTSRDLTIIGNDTDGKCVIGVIDNSDFITVKNVNITFENLLASGSILGVD
                     GYTGVPNSLLIKNCQIGTPEKTVANGVALRGNSPETPCGAVVMGCNIYAAGTGIMADY
                     ITKNAYINNVISIINPLADQTFYSGMYINGFAAGDTCVVFNNRIVNVEMNTTAPQFAG
                     GIVIGENGGVIDIFNNFIAMNTTNNGSATDNRVYGIGFSSANWSGEANIYHNTIDIAP
                     TNQTGVHAAIGTEIPFSAKLNIYNNIFSNRHDAANSFGIHWHNTASPNSVLASDYNDI
                     YIAGSSASVGLFGLIPRADLATWRAASGQDANSINKPVNFVSATDLHVTGSSIGDNDL
                     ACHRPAWLSTDIDGDARPNVTYMGADEGATALSFVGVANGSSGLPTEFSLRQNYPNPF
                     NPTTVIAFALPKSTQVTLMVYNMLGQPVAVLQNGFLKAGYYHVNFDASNLPSGTYIYR
                     IQAGDYRNVKKMTIVK"
     gene            complement(32815..34278)
                     /locus_tag="PKX09_03900"
     CDS             complement(32815..34278)
                     /locus_tag="PKX09_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU16997.1"
                     /translation="MRTNSDFIKKCLFVGVVIGLVFITTLFAIDNPLGREIQPRNLMN
                     QYNLTIYDLSGNPAFYGISYPDNLDFYQMQATNDNNVFRRLYDPRRQEDYALNFHSAK
                     HLSERNTLVTGIRFNRTIQRDLYHSLEKNIYDEYIAFLDTTTGTTTYNSPQINFLYDY
                     ALTKRLHLGLEINYGVEQSLKDVYTQCEIIARNTELRGGLSYISKNQKIIGGLLGTYY
                     NAQRKYEAVKYYQDAFVRTLFGYHIYKDETPKSINRKNDEREGGGLALQFCHRDFMLP
                     GLALIITGEYNGRSDNVTVGSPQKPTPRGHWVREIRRGILDLNYSPIESNNSLEFIYE
                     IRSTDDWVKSGNYEVIILDNAELLNVFSLRWQTRLFKRLKMNIGASYASQACDYQEYT
                     IPFQYHETDYHSSAFLDLNYAINQILSVYFLGNLAIDDLYFYWNAERATRYGCEIGIE
                     RLTTFGRFGAGLEYFQTVFDNPDPYNQSVGLKIFYWK"
     gene            complement(34275..35393)
                     /locus_tag="PKX09_03905"
     CDS             complement(34275..35393)
                     /locus_tag="PKX09_03905"
                     /inference="COORDINATES: protein motif:HMM:NF027540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4876 domain-containing protein"
                     /protein_id="HOU16998.1"
                     /translation="MVKKTALLTVILLLNLTNCFHQKPTEFDGIYTLKLVLVYRDSTL
                     GYDERLSNVDVKIHTGDYNLDDYCATTNDSGVVVFENLSWAIYDPQVKAKVELPFIDS
                     FGNRRLDSLISYTLIDTIVAITPSETGIGTDTIYTITANLRPGLKINEIYYTGPENRN
                     NWFYDQFIELYNSSDEVQYLDGMIVCRIYHMLTSVTYAFRFPGTPLTGREYPVQPGEF
                     VVLAQDAVNWLELYSTSVDLSGADWEFVNAMDFGDIDNLNVPNLANIVPGRTVDFMIN
                     LSTNVIALADGSDSVMTDGLDIGTVVDCVEYNSKSPSEQTTKNIDKILDAGWAGVGNQ
                     RYSGQSVERILPGFDTNNSFNDFVIIKPPTPGYHHPSP"
     gene            complement(35387..37993)
                     /locus_tag="PKX09_03910"
     CDS             complement(35387..37993)
                     /locus_tag="PKX09_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU16999.1"
                     /translation="MKKQVIIILILTGLAIRGIASTITGKVTCQMERKPIPDALIILQ
                     KRSDPDFSRTTVSDKTGQFTLTNVEPGVYNIEAVREGYFKNVLFDLKIEADRNYEINI
                     KLLKQAGKSSSDYCFMLGGIEVYSVQKELIPESPVTTRTINTGEIEHMQATSLGDILS
                     LVPGVDKSQNPGLSGRSEIGLRSVTAAGVSMSAVESFGSTIIINDNQISTDANTSGNV
                     YYGSATRGLDLRIIPADNIKSVEVITGIPSVEYGNFASGVIKVETKIGRIAPKFNAKI
                     NPDTKNASFSHGIKLGRSVLDYHLNYGYSERDLRKVGDEFQRLHASGNLSGAYLDQKL
                     ETHIGGSFTKLLDNEKPTDVYQMASYDKGFLATGDFNFIYKDSEAKSWKGVFSVNLNN
                     KRNYKSKWVAENYILKYDTTVVDSVTGIAIDTTLIKILPGFIGKMKEIGEEWKIAGKI
                     QRKIALTTKHTKHNLLFGGEVNYEKNTGDGVVIDENFPYYGQYSSHRSYAFDDYPDLT
                     SLSFYAEDVLEGKMLARRYKLMAGLRYDAYSPIGFNFKNMFKDKAFLKSEHGDFLSPR
                     LNLQYSITEDWQMRLGAGRSVKDISLGHIFKAPDYFKYASGDTIIEEVHLQYNPDLQA
                     YSIDKYEASLDWKFSDLIGFSLTGYYQASDNMPVTVSYPYGYEINPDTITAAAWERFE
                     NRGWSRSNGIEFTMRTQRIHNFQYRLNVTYRFNKNGKTGLVYDANPENWEDIWYKPES
                     YWSEKIIIDYQINYISNRLGIWVTLDAQQVPLEHFKRIYRSNSTYKTENGVTYLWYQG
                     MTYYYERYLRDTGGRWLFNFRVTKSLSQSMEISLFVNNIFDDRAVWKNFDGSLNEHNP
                     PIFYGLEVSAQW"
     gene            complement(38008..40788)
                     /locus_tag="PKX09_03915"
     CDS             complement(38008..40788)
                     /locus_tag="PKX09_03915"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOU17000.1"
                     /translation="MKWFRSLAYTIAIILIIFSSLLALDESRVKEFTLNNGLKVITYE
                     MHTAPVIYSQLTYNVGSKYEPFGQTGISHVVEHMMFKGTNRFPKGTISELISANGGIF
                     NAYTSTDITVYYELLPQNKIDLAFDIESERMYKCAFDPQEFESELKVIMEERKQRIEN
                     SAVGLRREEINTLIYKNHPYRNPIIGWMEDIRSINRDDAYKYYRTYYTPNNATLVLTG
                     DFNTDEIMQKVEAYFGKIPRGPEIPAYNFYRVPICGKRTLDFIHPDILSESIHYYFEA
                     PTRFEADGPALYVAGQILGARSSTSRLNKKLKREKQVCESAVAGLSFTKDPSTFNLIT
                     SLMPTAQISEVENLIWKEIDSLATTAVLEYDLQKIKNNTTFNELTGNQHNEKIGGQLA
                     TYDNFYNWRYINQWNQLVMAVTADDIIRVVNKYLKTGNYVACYSHPDTTQKKVGFLAT
                     ESDESEFDLEITEPETIEESEHTTDKYVNPEVRKIYFPKLDEVISPHPIAPLVTTAKL
                     KNGVPVYFLENHEYPTILLIGYIETGRLEEENLHPGIRQMTVGMLSRGTAKRTYEELI
                     EERSFIPYQIDISQSWNKISFQGYGLSKDVDKILQANFEIVTQPTFPESEIEKVRPSL
                     IKTAKEYKETDRMKAFYAMFEKVFEGHQYALPYAGDPETYRILTSADLKKFHAKYYAP
                     DNLKLLVVGDFNRKEMLSRLNATYGSWKHKSGDKHLPFQTVKPIQGKTVYVFSNPGYK
                     QCRVDIAFCPVEGGIKTDNPDIEALKLLESILCGSSLTSRMGKELRDKQGLSYSIKSN
                     LWIRDHGGYWNIRTELDQANLSKMIHGIFAEIEKVQKEGVTDEELEKAQARRIAMLTM
                     NTRTPDEIGAVIFDLIQEKKPLDYFDHSKERILAVTKADIQRVANKYLDTQNYIIAVS
                     GDMAPDALNEFK"
     gene            41336..41406
                     /locus_tag="PKX09_03920"
     tRNA            41336..41406
                     /locus_tag="PKX09_03920"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41368..41370,aa:Gln,seq:ttg)
     gene            41431..42372
                     /locus_tag="PKX09_03925"
     CDS             41431..42372
                     /locus_tag="PKX09_03925"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOU17001.1"
                     /translation="MKNNGIIFCGRSNPQLGYRINEILGIKPGAIKISNFSDGEITVK
                     LEDNIRGKDVFIIQSTNPPAENILELLLIIDAARRASARRITAVLPYYGYARQDRKDQ
                     PRVPISARLILDTIVAAGANRIMAMDLHSTQIQGFVNIPFDHLYAKPIFVEPLRKLIG
                     KRDFSIVTPDVGGIKVARSYAKILGLPITIIDKRRPKPNMSEVMNVIGEKDMKHALII
                     DDMIDTGGSVTEAAALLKEYGTETITAVATHGLFSGDCVKRLSDSPIDRVIVTDSVAI
                     PEEKHFAKLQILSAAPMLAEAINRIHNEESISSLFEL"
     gene            42389..43057
                     /locus_tag="PKX09_03930"
     CDS             42389..43057
                     /locus_tag="PKX09_03930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOU17002.1"
                     /translation="MVDYYKLNAELRSDFQKGVTKRMRRAGLVPAVYYHHTEKPVALS
                     LDLKDLKAALRSSARIYDLNINKKSHKCIIRDVQFDPLTEEIIHADFMGVSLEEMVTV
                     SIPINVTGTAIGVKTFGGILEQHLWELEVRCKASQIPDRVTIDVTNLNLGDSINAGSL
                     NIEGVEILIPATTSIVSVVKPTGTKAEEEVAKEEVTEEVGAEETAGTQEAKETKETKG
                     KSEK"
     gene            43083..43724
                     /gene="pth"
                     /locus_tag="PKX09_03935"
     CDS             43083..43724
                     /gene="pth"
                     /locus_tag="PKX09_03935"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOU17003.1"
                     /translation="MIRTIIGLGNPGKKYQHNRHNFGQMCLNHIARRLKLAFKPGGGD
                     RAFTYTTTVIADRTVYLCQNTTFMNNSGLAVRQILNQFDLQTDEILVVYDDIDLPLGK
                     IRLREKSSAGGHRGIRSIIETLATNQFARLRLGIGPQDEGVPSEDFVLTDFRPAEKEL
                     VEAVLEESYKCILTILNNGIRPAMEYFNRRALHLAPKENEIQVNSIKKPIILT"
     gene            43736..44083
                     /gene="rpsF"
                     /locus_tag="PKX09_03940"
     CDS             43736..44083
                     /gene="rpsF"
                     /locus_tag="PKX09_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOU17004.1"
                     /translation="MRYYENLFIVNPNIEQEKFTQLIQTVKAEIERLDGNILNLEDWG
                     KRRLAYPIQKHRYGSYVLAQFETENVELVKELESWLRLNQGILAFMTVALDEKPSPKT
                     TQPTDQPVDETIG"
     gene            44122..44358
                     /gene="rpsR"
                     /locus_tag="PKX09_03945"
     CDS             44122..44358
                     /gene="rpsR"
                     /locus_tag="PKX09_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOU17005.1"
                     /translation="MVLLSKRKICKFCENPEEKIDYKNYKVLRRFITEQGKIIPSRIT
                     GTCARHQRELTKAIKRARNIALLPYTYDVTQNFK"
     gene            44380..44823
                     /gene="rplI"
                     /locus_tag="PKX09_03950"
     CDS             44380..44823
                     /gene="rplI"
                     /locus_tag="PKX09_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOU17006.1"
                     /translation="MRVILIKDHENLGKIGDTVEVKDGYARNYLIPQGIALVANSAGM
                     KQMQEQIRLKELRHNKALKKAHELAEKLNTLSLTIPVQVGEEDRVFGAVTSQLIAEQL
                     QEKGYEIDKRNIQLEEPIKALGIFDIPIKIFQDVTAKVKLWVIKA"
     gene            44919..47360
                     /gene="priA"
                     /locus_tag="PKX09_03955"
     CDS             44919..47360
                     /gene="priA"
                     /locus_tag="PKX09_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOU17007.1"
                     /translation="MSAKVQYVDVIFPATLEKSFTYQAPSGYDGTLAPGQRVVAPLKS
                     SSAIGFIINLDPIVPANLKIREIQEIIDPQPIFPPELLTFLKKIADYYLTPFGRVLAA
                     AIPAEYRFIKTRTLKASRLDGAQPTEYGDLFAYIAERREVKYSQLKRQFETTYLQKGL
                     MALKKEGFIAELVEFKPSKYQGHIEKTVRLLNTDEETLSAIARKAPRQAEILNALQSV
                     GEPLKGRDLRRFSPVALNALARKELIAIDTTDRTLSDLWSEFGERRKTITLTEDQYQV
                     FQAISDSIKSAKYNAFLLEGVTGSGKTEIYLEAIRETLAQGRTALVLVPEITLTTHLA
                     ARFRGEFQDQIAIWHSRLTQAQRSAIWGAVLRGEFPVVIGARSAIFMPLPNLGLIIVD
                     EEQDNSFKQKSPEPRYHARDAALLRGVESQATVVLGSATPSLESQYNAIIGKLNKLAL
                     PRRYSKAPTAVIQVVDMKAEIQETGDVQNPFSRLLTAKIKDRLENGQQVLLLQNRRGY
                     SPVLLCPDCGWVPECRNCDIAMTYHKKSATLICHYCNLHETPPAVCPRCRSSNFLYPG
                     VGTQKVEYYLKTAFPDCRYERLDLDASRIRERAPRVLKQFEEGQIQLLIGTQMIAKGL
                     DFPNVTLVGVINADLGLYLPDFRARERVFQLLYQVSGRAGRGDIQGEIIIQTYNPQDF
                     TIRCALQQNLAGFSNFELNERNPLNYPPFSRMALIQISDLNNQRAATTAAAVADFLNA
                     HRGKIELLGPAVAPLARLLNRYRYQIILKSRREKDPNGRILRQLLSNFLNSREYRDLS
                     RQARIIIDIDPLDLL"
     gene            47357..>48087
                     /locus_tag="PKX09_03960"
     CDS             47357..>48087
                     /locus_tag="PKX09_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HOU17008.1"
                     /translation="MTPHRIFIVLIITYQILMATPPIKIVHPDGYEKLAEFTHTSILN
                     ALPNVPAIWQMTTDTIRIQIVARSADFEEAVGSDLPQWVGAVTLFPQDLMVVRSPDLS
                     RSTLQEYRRTVVHELIHLLQGQFVPLNLTPVWFNEGLALYYAGEFDLREQVVISKALI
                     RNQLIPPIKLEQILKFSQTQADLAYAESASLIEFMENVYGNGTAVAILARMRNGENFE
                     QAVLKLTDCEYDDLMNQWQTYVEKK"
     assembly_gap    48088..48187
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <48188..48402
                     /locus_tag="PKX09_03965"
     CDS             <48188..48402
                     /locus_tag="PKX09_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17009.1"
                     /translation="CEYDDLMNQWQTYVEKKYNWIFLLDIQNILWLIIPLIAFLAYWS
                     IRRRNKVTLEQWNDEEINENSDEQEI"
     gene            48419..49297
                     /locus_tag="PKX09_03970"
     CDS             48419..49297
                     /locus_tag="PKX09_03970"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOU17010.1"
                     /translation="MALVSAASAQALYQGLYQPIDARGWGMGSAMVAQTRNSSGVAYN
                     PAILAVVPNKWQVNYTRYVLDIQSTNALFVWQAPGKGKLAALGGYLDYGSFSERDDDG
                     NELGEFQVSDLNLRLAYGVPITRKLSAGLTAIFVNSDLANYNSKALLGSAGLLYYDPV
                     STFSVGLAYTNFGKMLSGYINDDEKIHSALMAGIAKKLEHLPMVIAADFMHYSSGENI
                     IKIGGEFILGQNYFLRWGTSSRRFEINTQQTITNFFSSSSLGGGLVINRLQIDLTWYS
                     LGHTGSVFAFSIAQNL"
     gene            49309..49704
                     /locus_tag="PKX09_03975"
     CDS             49309..49704
                     /locus_tag="PKX09_03975"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17011.1"
                     /translation="MRKTILILSTMTCVALVGLGLIGCEGDEANQPSYILDIDPIFGS
                     AKYNCKGCHTGAISPYLDLTTYEGLMAGSDNGKVVIPGDADHSFLYEKISQPIPSRGE
                     RMPLGGPFYLTSGEIRLIEDWINLGAKDN"
BASE COUNT        14096 a        10675 c        10319 g        14555 t
ORIGIN      
        1 gccaatgcac tgacgatggt ggatattaaa ttaacccgga acgcaatatc ctggctgaaa
       61 ggaatcatcg aaaaaatgcg accaatcagc aagaatagcg ggctacccgg aggatgcgga
      121 actccgagag tataggcggt agcaataaat tcgccacaat cccagaatgg taccgtcggt
      181 gaaattgtat cgagatagac gatgaaactg agcagaagcg taagcaaggc gagaattttc
      241 ttttcgatgt cttttttaaa ccagttcatg taatttcctt tatagactta ttgttttatg
      301 aatgagattc ttcattcggt ttctctgagg cggcactctg gtgtaattta gcctcaacga
      361 aattaatctt taagtcactc aaagcgtttt ccagaaagcg cttttcatca tcggagagat
      421 tgccttgagt tctgttatgg agcatttcca gcatatcaat gctcatagaa gcctcttcca
      481 gttctatgct attggttccg gttaccggat tcttcatttt accgagatga atccaggcac
      541 tactgaccag cgattgcact aaactaagga aaagaatttg ctgacgatca ggttgttttt
      601 caggcataat gtcacctata aaatattgct gaaatcaatt tgattagcat attgttgcaa
      661 taaatgattg cgagtagccg cattctcgcc gcggcgtaaa tggggatcgc ttttcaaaag
      721 cgtaaaggcg cgcacgcgag cagtttttaa taactcaatg tcagtcaacg ggtcggcaat
      781 tttcattttt aaatatccgt gctgtcgtgt gccataaaat tctccaggtc cacgtaattt
      841 aaggtctgcc tcagagattt caaagccatc aatcgtcgag agaattgttt taatccgctg
      901 atgacccagc tcggtaacct tgcgctcgac cagaatacag acaccgtttt tgcgaccgcg
      961 accgattcgt ccgcgcaatt ggtgcatctg agtcaaaccg aatcgctcgg cattttcgac
     1021 tagcataata gtggcgttgg gattgtcaac tcccacttca ataacggtcg tacttaccag
     1081 caaatcaatt tttccctgaa tgaaatcctg cataatgcgg tctttttcgg cgatggacat
     1141 cccgccgtgt aaaagcgcca gtcgaaattg cgggaatact ttttcactga gatattcata
     1201 gccctgaatg gcggcttgca aatcagtctt ggctgattct tcaatcaaag gatagacgac
     1261 ataagcctgg ttacctttcg gtaattgttt ggcaatgtaa tcgtaaactt tcccaatttg
     1321 gtccgccgtt acagcccggg tgataattcg ccctttacca gccggcattt catctaatac
     1381 ggaaatgtcc atatcgccgt aaagtgtcat actaagggtg cgtggaatcg gcgttgcagt
     1441 catcaccagc acatccggat ggtagccttt gctaatcaag gtgctccgct gaacgactcc
     1501 gaagcgatgc tgttcatcta taataaccag tgccaggttt ttgaaagaca cattttcttg
     1561 aatgagagcg tgtgttccga cgattagctc cgcttgtcca gcctggattt cgtcgagaat
     1621 tgtctggcgt tcctgctttt tttgaccgcc gattagcagg cggacttgga tgccagccac
     1681 ttcgccgagc tgcttcagga cgcggtaatg ttggtcggct aaaatttctg tcggagccat
     1741 caccgccgct tgaaagccat tgccgattgc aatgctggcg catagcaggg ctacgacggt
     1801 tttaccactg cctacatcac cctgaagcag gcgattcatc aacgtcggag aatgcatatc
     1861 attccagatt tcacgaacga cttttttctg ggcgttggtc aattcgaacg gtaatttcgc
     1921 ataaatttgt tttaaaagcg gtccggcaga tttatagcgc tgaggctttt gaatggcttt
     1981 gattccggct cggcgcaccg ccaataaaag ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnngtttcgc cgaccttgaa agcatttttg atgtaattaa taccgttgaa
     2161 ccagaccgct gtcataatgc cgctaccgtc gctaatctcg acctggaaaa acggtcggcg
     2221 ataacgccga accaaattct gcgaaattat ttttcctacg acggtagcgt ccataccgac
     2281 ttttaactga gcgatttccg taattgtact acgatcgaga tagcggcggg gaaaataata
     2341 tagtaaatct tttaaagtgt taatgccgac cttcgccagc acttcggcgc gtttcgggcc
     2401 gacgcctttg acataggtaa tcggcgtttc cggtcgcaga tcaacagttt tcttactgga
     2461 cggtcgttcg tttaccaact tctctggaag gaatacttga tgatgatatt tttatcggcg
     2521 gggacagtca atggaaaaac cagcagacta ttgcttttct tctggtaagg atgcgacgca
     2581 tttttcacaa accaatcgcc gctcattatt tcctgaatct cgacgttgat cggctccgtg
     2641 cgcttattcg aaatttccaa ttttatcgaa attatttccg aacggtcgct gattttctgg
     2701 cgatcggtga ttgttttttg gcctttgacg tcgaaagcct tgccgactat caagtcgagg
     2761 gtatcgtctt tggcggtgtg cgcaatccga tcttcgccga ctaattgcag ggtattatca
     2821 acatcctttt tgaaaatacg gaaaacgccc tccggtagcg gaataccgag atgattggcg
     2881 gttgaattgg caaacttcaa atggacttcc agcggtccct ccgtttcgct aaaagcggaa
     2941 tttttgaata gataaaattt ctccgctttg gcgttaactt cgtcgaataa ggtaatttgt
     3001 tttatttccc gatctttaat ggttacggga cgctggagct catacagatg gtactcgaac
     3061 aagccgcgtt cttcaaaggg tgcgacttgc gtaaggacat cggcggcata gactttctct
     3121 ctgcgaaccg gcgccggttg gggagcgcga tgaatatctc cggcgaccag cttcatcttg
     3181 gcgttttcgt aagtggcgcc ggattgatta tcgagcgaca cccaggaatt gagcgtaaag
     3241 tcccggtcat ttttatctag caccattaaa tattcggcgt gccagttcat tccggcagtc
     3301 aaatagctga tttcggattc gactgcggcg ttgattctcg acgacaacag ccattgtaaa
     3361 gtcggtttta aaatcaatcc ggacggcagg gatgggaagt catactcgga aatattttca
     3421 gccgatgcga tcttgatgcc accatttgga agttgtagga tgagactacc gccatcgaat
     3481 tggagtaatt ttccattggt ctgttcgcca gatttggtct tatacgagat cgactcaccg
     3541 atatattttt cgaatatttt gatagaattg accagatcgt agagaaaatt ctgctcgagg
     3601 acttcgatag cgtcggcttt cccaggaaat gtaatgcgca ccgaggtcgg atcaacttta
     3661 gccggaacgc cttcaatgcg aatttccgaa tcgcctttgg cgatattgag cgtgcgagtc
     3721 tggagtacca aaccaaggtt gttattataa acggtaattt gagtggactt gacgggcgta
     3781 tcagcgaaaa ccatcctaat aagtcccaaa atgacagtcg ctaaaagtat tccatttttt
     3841 aactttttca tacgactctc ctcaatttga tttaaaaata atcagcgaat gtgacttagt
     3901 tattcagcaa cttaaggttc gctttgaatt tatctaattg ttccttcaga acttgctctt
     3961 ttgcgcgttc tttgtttata atttcctgcg gcgcacgggc gaggaagtcg cggttggata
     4021 atttggcaga agcgcctttt aatattccgg ttaaacggtt gatttccttc gtgaggcgag
     4081 cagtttcggc ggcaatatca attacacctt ccaatggaat gaaaaactcg gtgccgcgga
     4141 caacgaccgt tgaagaatga gccggtttga cgttttcggc aacgaacgtt atttgggtaa
     4201 ctttaaccag atttttaatg tagttttgaa tttcggaatt gttcagtagt tcggtgaccg
     4261 gcgaactgcg cgcagaccga acgatcaatt cgatcgacac agccggtgaa atgttcattt
     4321 cactgcgggc ggtgcgcaaa gcggtgacga tttcctgcag gagttcgaaa cgctcagtca
     4381 gggctttatc cttggggtga tattcgactt ttggccaagt tgagacgacg atgtccggct
     4441 cgttaggcaa tttgatatgc tggtagattt cctcagtaat gaagggtgca taagggtgaa
     4501 gcagtttgag gaaatttttt agaacataga ccgccacccg caaggcggtt tgtttctcag
     4561 ccagtgattt tttatatagc cgatccttga tgagttcaat ataccagtcg cagtagtcgc
     4621 tccagacgaa atcatagatc gtacgcgcaa cggtatcgaa acggtacttt ttaagatttt
     4681 tgtcaacgga gcgaatggta tcattcaagc ggcttataat ccactcatct gacaattcca
     4741 gttgcatacg cttcggctcg aaaggttcac cgcttagcgt attttcatcc agattcatca
     4801 agacaaagcg ggcggcgttc cagaccttat tcataaaatt gcgtccgact tccagccgtt
     4861 cctcactgaa taaaatatct tgaccttgcg gggcaattag cataatgcca aagcgcaggg
     4921 cgtcggcgcc atatttctcg actaaaacca gcggatcggg agaattgccg agcgatttac
     4981 tcattttgcg gcgctgagcg tcgcgaatta tgccggtgaa atagacattg cgaaaaggaa
     5041 tttcatgaca gaactcgagt ccagccatta tcattcgcgc tacccagaag aaaatgatgt
     5101 ccggaccagt gacgagatca tcggttgggt aaaatttgcg caaatcgggg gtatcatccg
     5161 gccagcctaa cgtcgagaat ggccacaacc aggaactgaa ccaagtgtcg agtacatcct
     5221 catcttgacg cacggcgccg ccgcatttag gacaagcgga cgggtcttcc atcagagcat
     5281 cgctccagcc acaagcgtcg cagtaaaaaa ctggaatgcg atgaccccac cagagttggc
     5341 gagagatgca ccagtcgcgg atattggtca tccaatgttt gtaaaccttg acccattttt
     5401 cgggataaaa acgaattttc ttttcctcga cgacctttag cgccggttct gccagcggtt
     5461 gcatccgcac aaaccactgc tcggaaagat aaggctcgat cataactcca gcccgctcgc
     5521 tgtatccgac ggaatgcgag tactcctcga ttttttccag caaaccgagg cgttccattt
     5581 cagctacaac cagttgacga gcttcgaaac gatccttacc ggcgaccagc ggcccggcgg
     5641 cttcattcat cgtagcgtca ggattcagga tattaatttt gggcaaattg tggcgctcgg
     5701 caatttcgaa atcattcgga tcatgagccg gggtaacctt aacggcaccg gtgccgaatt
     5761 ctcgatcaac gaattcgtcg gcaataatcg gtattattcg cccgataatt ggcagtttga
     5821 catttctgcc gactaatttt tgataacgag aatcagcggg attgaccgcc acggctgtgt
     5881 cgcccaacat agtttccggg cgagtagtag caacggtgat aaagccggaa ttatccgcca
     5941 gtgggtagcg gaaataccag agatggcctt tggtatcgcg gtgaaccacc tcttcatcgg
     6001 aaagcgccgt ctgcgaagcc ggacaccagt tgataatccg ccgaccccga tagattaaac
     6061 ctttatgata aagttcgacg aatacctgac gcaccgcccg actgagccct tcatcgagag
     6121 taaagcgctc ccgctcccaa tccagcgaac agccgattct gcgtaactga gtcaggataa
     6181 tatccttatg cttaagcgcc cagtcccaga cgagtttggt gaattcttcg cgaccataat
     6241 cgtagcgagt cttaccagct ttgcgaaggt tcttttccac tacattctga gtggcaatac
     6301 cggcatggtc agtgcccggc aaccacagag tttcaaaacc ctgcatacgg gcgtgccgga
     6361 tcaaaatatc ctgcatagta ttgttcaaca cgtgcccaat cgtcaatatg ccggtaacat
     6421 tcggcggcgg aatgacgatt acgaaaggct ttttaggtga atcggctttg gcgtggaaat
     6481 aatttttcga gagccagtag tcgtaccatt tggtttcaac ctggcgcgga tcgtaaactt
     6541 tatcgagttg aatagacact aaaacctcta attactgaaa atcataagcc tattttcttg
     6601 aatcggctta acaacgaact taatgaaatt aaaataattc acaataactc ataatcaaat
     6661 ttttcatcga gaaattttac ttaaaatatg ctttaaagtc aatcacggat ttggtgaatg
     6721 agcggaattt accacaaagg cacgaagaca cgaagacatt aatgcaaaaa tccgaaggct
     6781 ttgagtggtc ttctttatcc tctccgagga atagcttttc gatctccatt gatctacaat
     6841 accttaaccg ctatgcggta tccataatta ctactgaatg actacgaatc gctatcaaat
     6901 aaaatacttc tatgattttt tgaccgcaga gcggtatttg gtaatttaac gaccatacta
     6961 tttgacctgg gtgggttaaa ccttcattaa attgggataa gtcacgattt aaaaatatct
     7021 taccctcaaa ggatttttta aaataggaaa tttaaattaa gttatcgttg gattttagaa
     7081 gaggttatca aatgaaaaag aaaagtaaaa aatacaaccc cttacagatg gaaaaggaat
     7141 tgtctgatct aattgagtat attgagaatg ccgggtttga atcgaaggaa gagctagatc
     7201 gctttttagc gaaaatggga ggtaaaaaaa ttggttcagt tctaaataaa aagaaaacag
     7261 ccgcagacga agcatttgaa ttgattctcg aagcccaaga agtatcgcct gaggaaggaa
     7321 ttaagctggc aaaaaaggct ataactttgg acccgaattg tgccgacgct tatactttta
     7381 tagccgatca tactgaggat gaagatgaag ccctagaatt atatcaaaaa gcgatgcaag
     7441 ttgcgcgtga atctttagga gagaacgcct ttattgaata tgtcgggcat ttttggggaa
     7501 ttaccaaaac ccgtccatat atgcgggcta aagccggtta tgcgtcttcc ctaattttaa
     7561 aaggtgatct tcaatcagct atcaaggaat tccaggaaat gttagtcttg aatccctccg
     7621 acaatcaggg gattcgattt tcactatcag tgctgttgct ccaattgaac cggcttcgtg
     7681 agtatgaaga tttattgaaa caatataaag atgaaaatag cgctggctgg ctatataacc
     7741 gcgtcctcta tttatattta aaatatggag gcactaccag aacgatctca gctctggaag
     7801 aggctatcgc cgagaataaa tatgtaatgg atttgatcac cggtctcgaa gaaccgccga
     7861 aagaattacc ggaaagctat ggcatcggta gttacgaaga agccgtctta tattactggg
     7921 aatctatttt aacttggatg gcgcataaaa aagccatttt atggcttatc cgttactggg
     7981 agcgacgccg ggaaatcaat tgaattaagt cctatcgaat ttctctaaaa atagcagaat
     8041 aacgttaaaa aacattcagg aatatttttc ggatttggtg agtcgctgaa ggtttaacaa
     8101 taaatcaaaa agtcaaaatc agcctttaaa tacttggttg cgaaaccaga ttgatattcc
     8161 ccagaaaagg gcaattccac cagcaagaag gacaaccaag tcgagaacac atagtattcg
     8221 gagacagctt atattaaaaa ttttgagttc gccattaact tgctgtacca ataagtaaag
     8281 gcaaatcaga gaaaccgaaa ggacaattaa ccctccccca actaaaaccc gcgaccggtg
     8341 ccgcaaccgc cttggtaata tttttgccat acactccctc gctaaaaacc ttacctaccg
     8401 ttaattaaaa ctaatacttt atatcttaag gagaatctgg agggaaaaca atgaaaaatt
     8461 taatggatta agataatcct aaaacaatta gccggcttta atccttagcc agagtaaaag
     8521 cccattcatc agcatgaaaa acccgactat acaccaaaga atcgccgcca gcgctacact
     8581 tcgtacaatc caaagaccga ggtaggtaat taatgcttgg tgtgccagcg aaataataag
     8641 tacgaaaacg attggtagga aaaaattaat taaaccactt ctcagagatc ttttcagctt
     8701 ttcccggtta tcgcggtggt cggagtggtg ttttcttcgg tgtcgtttag tgtgctccat
     8761 actatctctt gttaatgccc attataaaat aaaaaaaatg ttctatttgg tcaagctaaa
     8821 gttttttatc ctgcttaatt tataaatcca taaaaagctg ttgatacagt ccggttttgt
     8881 tattttatag atcggaatat gaaaatatcg gtgaaattgc gaagtgagtt gggaaatatg
     8941 atcaagaagc ggtgttaaaa actttgagca tccagagaaa atagaagatg acacaaatca
     9001 attggtttgt ttcagttatt aaataacaca tcataactta atacattaca gtgtattaaa
     9061 taaatatctt tattcttatg aattattcag ggtagaaagt aaatctttca ttgccaagtt
     9121 ttctctttat gccaatacta caattagcat taaaatcatt ttcgaattat attaggaaat
     9181 cttcttagga aatcttcaga atgtttattt atggtcctta tctatttata gatctccgcc
     9241 tcaactttct gaaggtggaa agggctacaa ttttactcct caattttttc cgtgcttgat
     9301 tactctgccc tgtactataa aaattatatc ctcacagata ttggtcgaaa ggtcggcggc
     9361 gcgttccagt ttgcggctga tgtccatcac ccgtaaggca atcggcacag ttgccggttg
     9421 tttgctcatt aatagagtca tctcggcaat tattttatcc cgcagatcgt caaccacatc
     9481 atctcgtttg cagatttctt gggctgattc ggcatcatta ttgataaagg catcaatgct
     9541 atcatgaagc atcccgagcg attcctgcgc caggtgattt agaagcggga tcggcttaaa
     9601 gctcggtcta tcaatcaggt aaagagcatc acgacaaata tgcactgccc catcaccaag
     9661 ccgttcaagg tcattactca tttttaaaat cataataatt gtgcgcagat cacgcgctcg
     9721 cggctgatac tgagcaatgg tctgaataca agcttcatcc attcgcaaat cgaactcatt
     9781 tgcttttgat tcatcaacat taatgatttc tttcagcgta ttaacgtcct tagcactcag
     9841 cgcttgcaga cttcttgtta tcatactctc gactaatgcc gcatattcga ctatggcttg
     9901 ttttaattct tgaattttct tcgttaacat attttacctc atcatatttt ctattaacca
     9961 aacttgccgg aaatgtaatc ttcggtgtgt ttattttccg gtatggtaaa aatcttctca
    10021 gtaggcccaa attcgatcaa ttcgcccaaa tacataaacg ccgtataatc ggagacgcgc
    10081 gccgcctgtt gcatattatg cgtgacaatt acaatggtgt aacgctgttt caactgctcg
    10141 atcagttcct cgatgcgctt agtcgaaatc ggatcgagac tggcgcaggg ttcatcgaac
    10201 agaataacct caggctccac tgccagacag cgggcaatgc acagacgctg ttgctgacca
    10261 ccagaaagcc gcaaagcgct ttcattcagg cgatccttga cttcgtccca gagtgcggct
    10321 tcctctaatg agcggcgaat ggtattttca attgagtcgg tacttttaat gccattaatc
    10381 ttcaatccgt agctaagatt gtcgcgaatg cttttcggga agggatttgg tttttgaaaa
    10441 accataccga ctttacggcg caaagttaca acatccacac ccgggtcgta aatattatga
    10501 ttatcgagta gaatccgacc ttctacccgg gtattgggaa tcagatcatt catccgattc
    10561 agtaaacgga tgaacgttga tttaccgcaa ccggatggcc caataatcgc cgttacccga
    10621 ttttggaaaa tcgacatatt gacatttttc aaagtctgtt tatcaccgta gtaaaaatta
    10681 acattttccg cctgaatacg gatttcctta gacaagttca tatttcctct gtttctgccg
    10741 taaataaatc gctagccccg aaactattaa cactagaaat aaaagtatca aagcacaacc
    10801 ataggcaatc atcgtctggc gttctggaaa agtaccttcg gtcatcaggg cataaatatg
    10861 ataaggcaaa gccataactt catccataat tgaattggga aaaccacggg tgtaaaaggt
    10921 taccgcagtg aacataatcg gcgccgtctc gcccgccgct cgaccgacgc ttaggataac
    10981 tccggtcaat attcccggca ttgccgacgg tagtacaatt ttgcgaattg tctgccactg
    11041 cgttgcgcct aatgccagtg atgcctctct aagagattgc ggcacggcga ttagcgcttc
    11101 ctgagctgcg gaaataattc ccggtaatat cataatgccc aacgttaagc taccggataa
    11161 aattgaaact ccaaatccga aaaactttac gaagaccgtc aatccgaaaa gtccaaaaac
    11221 taccgacggc acccccgcta agttattgat gctcatccga ataacgttga tcacaacgct
    11281 tttcggactg tattctgtca aataaatggc gcatgccaaa cccatcggca aagcaaatat
    11341 tatcgcgccg attgtgagat aaaaggtgcc gactaaagcc ggtgcaatgc cacccttggt
    11401 catcgcccgt cgtggcactt gcgtgagaaa ttcccaatta atgaccttaa taccattgac
    11461 cgtaataaaa taaatgatcg cgccgagggt aagtactgcg ataaaaatcg ctaaatataa
    11521 aaatccaaaa ccgaaatact gtatgcttga atttcttttc atcgtcccag ccccagtttc
    11581 aagcgataat tgcggctaat aatttccgca atgatattga aagccatcgt aattagaaat
    11641 aaaactaagc cgatggcgaa tagtgcgtga taatggggac tgccgactac ggcttcgccc
    11701 atctcagccg caattgtagc cgtcatcggt cgcaccggat cgagaaagct tttcggaatt
    11761 accgccgccc cgcccgcaac cattaaaact gtcatcgttt cgccgactac tcgactcatc
    11821 ccgagtataa ttgccgttga aattcctgaa ccggcggcag gaattatcac gcgcgtcaga
    11881 gtttgccagc gattagcacc taacgcataa gaggcttccc ggaaactttt cggcacaaaa
    11941 ttcaaggcgt cttccgccag actacaaacc gtcggcaccg ccattactcc gagaatcaag
    12001 ctggcagtca aggcgcattg tccaatcgga atatcgagta tgttttgcag aaagggcgca
    12061 atgattacca tcccgaaaaa gccatagaca atcgaaggaa tcccagccaa aatctcgata
    12121 atcggcttca ggatcgaacg caggcgaaat ccggcaatct catgcagaaa aagggcactg
    12181 ccgattccca ctggaatgca aaataacatc gctcccagcg taatccataa agtacctaaa
    12241 attaatggca gaatgccaaa ttcagccggt tccgccgtcg gataccacgc cttaccacca
    12301 ataaattcaa aaaggttgac aatacgaaaa atcggcaaac cctgttgaaa tagaatcaaa
    12361 ataattccca gtaaaaacac caacgaagca aatgccaatc caccaaatac tggttttact
    12421 accttttcgc tattccattt catatttagt tctcataatg acggcatggc agacatttgc
    12481 ctgccatacc ataaattaat actatacaaa aaaacttccc gatactattt taatccaaca
    12541 tatccggctg tttcgactaa ctcttgccct tcctcgctga ggataaagtc aataaaatcc
    12601 tttacgacgc ccttgggctt gccattggtg tacataaaga gtggccgcga aagcgggtaa
    12661 gtcttagtca aaatgttttt cttggtgcag gttacattct caacggtaac agccttgatt
    12721 ttgcttgata tataaccgat accgacgtag ccaatcgcac ccggcgtctt ttcaaccgtg
    12781 gttgcaatgg cttgattaga ggcattcatc agcgcatctt gacggacttt ttcgtcatta
    12841 agagctagtt tactgaaagc ttcgaaagtg ccgctggcgg tatcacggga cagcactacg
    12901 attttcttat catccccgcc aacttctttc cagttcgaga tcttaccggt gtaaattttc
    12961 cgaatttgct cgacggtcag attgttaatt ttattcgagg gatgcaaaat aactgcaatg
    13021 ccatccatag ctacgatatg tgccactgga ttgacacctt tggcaacggc atttttaatc
    13081 tcctcatctt tcatatcgcg cgaagaagtg gcgatgtcac aggttttatc aattagcgag
    13141 gcaatgccaa ctccggagcc gcctccctgt accgagattg tcacgtccgg attagcaatc
    13201 ataaactcct cagcggctaa ctgcgcgatc ggcaaaaccg tggtactacc ttctatacgt
    13261 attttgacag tttcaccagc cgttaacagc gacgcaattg ccagaatcaa aattgatagg
    13321 ttaaataatt tcttcatttt tagttccttt ctttgttaga atttaaattg aatgtcagtt
    13381 tggagaacgg tttcaggttt aatcgctccg tgtaggttct ctattttata gtaatcgagc
    13441 gccaggacga tatttttatt caaaccgtac tgggctatca gttcgctccc cttaatgtcg
    13501 gtcttaccac cataagtatc ggaatccggt agaaaatcta accaagcgtc cttttcaagc
    13561 tggcgataaa catatttcac ttgccactgg cctttgtctt tgatcttttc ggaacccaaa
    13621 gttaaaccac aagctatgcc atttttagca gtgtccgatt ccgcctggag gttttgatta
    13681 atttcaaaac agacaccgat atagggaaag attttgttgt atagggcgac ttcggtagca
    13741 aaattgaagt tattgtagcc caacgccaga tatttagaat cattaatggt aatataagaa
    13801 ttagaccatt tggcatgatc gaaagccata ttctttgttt tattgaaatt ataataggtc
    13861 gcggcggctt ttaaactgta tataccttct ttagccagat tgaaaccggg ttgtaaaatc
    13921 agcatacgcg gattatcggc aatgacattt tctaccttgc ccttttcatc cagaaagtaa
    13981 attccagtat taaagaatat cattttatga ttgaattgcg ccgcaacgcc ttcgacggta
    14041 atatcggtat cccacatata atcggtcggc gtccagaaac cgaggttact gtaaaatttg
    14101 ccgagcagaa cgttcgcata tttcggaaag gtataactac cgtaagcgta gtccagcata
    14161 atcggcttgg tttcgaagga attgtccagc gtctgattgg tagaacgcgg atcactggaa
    14221 ccagttgcca atccgaagcc aactttgaat cccgacgtca ctacggcttc accattcaag
    14281 cggaagcgaa tccgctggcg catccgcgaa acggaatctt cttcggtggc ttcccactgg
    14341 ttgcggaaac gaaaatcgcc tttcaatccc atattctgaa tccatttcgg taaagtggta
    14401 gaatttccgg cattcatttc cactcgcatt tcttcttcgg tcaaagtcaa aatctcctga
    14461 gctttaccgg catccaacac gcccttctca actaattcat tgactaatcg gtcaagttca
    14521 ccggcgttta gcgttagggc acaaaagccg agcaacgcta aaataatgtt gactttcctt
    14581 tctctcaact tcatttttac tcctttttgt tggttacttc atttttaacg ccgataatta
    14641 aggaattcat tttaagactt ggtaaacgaa cggttaattt ttggttaaga attatttctt
    14701 acaacgctgg cacagtgaaa agaaaggaaa aaggctgaga atggccatta gtcattggtc
    14761 attggtcatt agtaaagaaa gttgaggcag agaaaagaat tttaatctta gcttttcttt
    14821 taggttaaga ctgataaaag tcattactta gccaaaagaa agctcccacg aggcggacta
    14881 tttgtaatca aagagaaagg gaaactgatt ttcgcttctg aattgatttt ttagccttta
    14941 ttgacttccc agatttcgcc accgaagtaa tcccagggca gacgccattc gtcacactcg
    15001 gacggttcgg gagaaaattg cttattcata aaatagatta ggtgtgcttt tttgagtccc
    15061 agatttttac agccgtgagc cacgcccggc ggaataacga ttaattggga attgtcgtcg
    15121 ccgatgacca aacgcattct ttgtccatta gtcgccgaat cctcccgcaa atcaattaat
    15181 atgagcaaaa tcttactgtc cggcggaaca aaccagatgt cggtttgctg acggtggacg
    15241 tgaaaagctt tcacactctg cggttccatt tcgctgaaat tgatctgcgc aattttaaat
    15301 tcctcgactc cgctaaaatc accattagtt atgcgcgcca gctcagtaaa tgaaccgcca
    15361 tcgtcattat ggcgccgcag attaatcacc ttgactccct cgatggttgg ctttccagcg
    15421 taatcctgca agtgcacgat ttgttcaatt tcttttttta actgtacttt caatttatgc
    15481 aacccctttt cttcaaaaat tagataactg gtagaaatta tcacattgtt acttaaaaaa
    15541 ccagataatc cgcttcaggc gcataggaat caagactttg ctcaatatgc ttatctgcta
    15601 tctaattgat aatccgaatc tgtatcccta cagggaataa atttccgaca aatcttttac
    15661 cttggagcga ttaagctttc taataaatta gtgaaagtct tgtataggat tgcttgacat
    15721 tcgcctattc tgctggtaaa ttttcgacag tttttctaaa tatatggcga tcacagtttt
    15781 cataatatct ctatttatcg gcatattcgt actcaatttg ctgattttaa ggtacttaaa
    15841 aggatcaata cataaatcat ctctttcagt tcgtctgcct aaaacggtag cggctttcga
    15901 tcgctatact ttgaaagtcg tggcgctcgt taccttgata attatcattt ttatgttaat
    15961 tgccatagcg ctccaatctg gatttgttat accggtcatc cagcctatcg cgctaatttt
    16021 attcattttc ataacggggg ttggctttgt atattttttc aaaagtgaaa gaaatatctc
    16081 aaaatctgac cagaaatttt ctaccggaga gaccgacggt gaggagcaaa agtgaaaagc
    16141 gaaaatgtcg ggaattggca acccttacgt tttatatttc cggataacac cgccatatcc
    16201 tttgacgccc gcattgcgga ttcagtaatc aaagaactag cgctggatac tggtcaaccg
    16261 gttttcaaat tgagatgttc aataaatgaa ctttatcagc cgaataccca aatttttacc
    16321 agcggtttcc cttataatga cttgtggttt cgctatgctc aaatattagt aatcgaaaag
    16381 catcttaaac ttcaggcgcc ggttggtatt gaattaatcc gcgtaatttt ctgcgagttt
    16441 ctcagaattg ccgaatatat ttttaattta gctacacttt gcttaaatgt gggactgaac
    16501 gatatttatt ccggtcttat gcaacagtac gaatccattc agcaatattt cgagcaaata
    16561 ttaagtggaa ctaatccgat ggattttatt attggacata aaacaccaga agacctgacg
    16621 gtcggattta ccgaaatgac tttgaatctc tcgcaccagc tccagattca actccggaaa
    16681 tctttacctc atttactcaa caaccgcctg attctttccc gatttagcaa aatcgccgac
    16741 cagaccgctg ataattcagc cattttaaat gataaattag aattcgatca aatgcgattt
    16801 acttccagta aaaaacaaaa taatgtttgc tggcaacttt tgaaaatacg ttgtgacgac
    16861 ctatttcgat caatcggttt gattcggctg tctctaacga aatttgttta tcttgatcgg
    16921 tcgccgcaaa cactgccttc gctaacttct cttcccaaag ggaattcgag tgtaaatatc
    16981 gaaactcctt ggggcaaatt gtcctgccag atgaattgtc cgggtgacag aaatccgtgt
    17041 gattttatac tatcaaatcc gcttcacaaa atccaacttc tgttatgcaa aatggctgtc
    17101 gggctcgagt tggacgaatt accgcttctg attacgtcac tcggtctccg accgaaaata
    17161 gagccacgtt gatttaaatg ccaatggtta tatgaatatt ctatttaaaa atttgatata
    17221 tgggttattc tgggtagtga ttttcgtagt cataatcccg gcattatcaa ttattgaaca
    17281 acggtatttt acttgtcatt ttcgattaac gggaaaaaag gcgaaaccga ttccaagtta
    17341 tttgcaatat tggggcaaat tttggtcaag tctccggggc gaaaaattac ttttggctat
    17401 gactactcaa gtttttttat ggattgaaac cggttattta atggctcttt ggttagtttt
    17461 gccctatcaa atgcaaattc agggtaatca gacgttatgg ttattcctgg caattatcac
    17521 cttaccgctg aatattatca attcaattat cactagtgaa gaggcaaaat ccgaaatcag
    17581 ccaggctgga attttcaaaa acggaattag ccttataagc agtttcgctc cgttgcttct
    17641 gtcaatttta attcttatca cgggtaatcg ttacaccagt ttttgggaaa tatcaagcca
    17701 acagagtggg taccgcttgg gttttattcc aaattggact atttttagtt cgccattatt
    17761 cttgcttatt ggaatcattt ttctgataaa tttgacgatg ctaatgcgcc ttagcgacgg
    17821 cagttataaa ccgttggaat atgaagaact ggcagagatt tacagtgggc ggtacgaatc
    17881 tttcggcata ttgccgcaat ttaaccgttc ggcgatgctt tttaccctga ctactttggc
    17941 gatttttcta tttttcggcg gttggcaaac gccgcttgaa cctgagggac aaaatttagc
    18001 ctcgttagct ggtggactta ttttcttcgt taaaacaatt ctgattttca gcgcttgtct
    18061 ggcaattaag taccacttgc caattctcac taactggcaa attattaaga ttaaccttag
    18121 aattcaaatt ccagttctaa ctttgatctg gctgggtttg ttgatcatta ggtggctctg
    18181 atgacaaatt ttcaatggat gcctgtcatt ttttggttgg cgactttttt gaccttgctc
    18241 ccagcgggct tggcaattac ttcaacgaat cggaaaagcc tcatcctgtc aaatatgttt
    18301 actcaattag gtgttttcct actgataata agtctgggag tcggctcccc ggctgttatt
    18361 ttcctcgttc tggttttccc attaaatata atgatgcttt atggacataa tttattcccc
    18421 gaaacaaata aacaaaccgc cagagaatca cgcccggcga tttttctaaa aaccgccgta
    18481 tgctttttgc tttttttact tttaattata gcattttatc aaaaccatct gaaaaatatg
    18541 ttggtcattg ataattcagg aaagattctg gaaggcgtta tccctgatgt ttctttatta
    18601 ttgattttga tagggatgtt tataatgtta atcctagtcg ctatcagcca ttttttaagg
    18661 agaaaatgag ccaccttttt ttatatcaaa ttgttggtat tggacttttc attatcggtc
    18721 tatatggctt gtggcgcaat cgtcattgct gggcagtaat tacttttttg caattaatgt
    18781 caagcggcat cttacttagt tttttagcga ttatccggac aaaaggcgct tatcaatccg
    18841 tgtcagaatt gattgtaata tttatttttg cggttctgat tattgaaata cttctggcaa
    18901 ttgtttttac ttataaagtt cttatgtccg actcatcttc aaattccgac gacatatcca
    18961 atcgaggtta aaatgaatat tttgatcatt ctacttttgt tatttccggt ttgcgcctat
    19021 ttgcttactc gatgtctgcc ggataaatat ttattgaatc ggcgctggct gtcgatcctg
    19081 tttttagtga tttctggatt aattgggctt ggaatattca tcgttctcct tctgataaac
    19141 atgcccgatt acgttcaaag gatttcttgg acttgggcaa atattgccgg tcactcgctc
    19201 cagatttcat tattgctgaa taatttttca ggtttgctat tagcgctttg ggcgcttggc
    19261 agtttaattt ttgcgatttt gactgccact gatttttaca gcccagatca tttccgtgaa
    19321 aatgttacca atcttgcgct gggaactttg gcggtcaacg gtttgatttt agccaatgac
    19381 tttttatcgt taatcttctt ctgggcgtta attgatttaa gtatttatcg agttattttt
    19441 aggaaatttg ataaggcgtc cgagtcatat ttgccccaca ttatggtaat cggtggtcat
    19501 atattactga ttctcgccgc cttttcattt tataaaattt gcggcacgtt ccgatttgat
    19561 ctgattatcg aagaggtatt atcgggaaaa gttgacccac agcaactaat attgccaggc
    19621 agtatgctca ttctggttgg aatcttgcgc atttattcgc ctttctttcg ttatcagttt
    19681 aatagtcaga cgaaaatgcc gattagtgat gaattcatcc ttttttgtgg tttgatgccg
    19741 tcgggcttgt tcctggtgtt aaaattattc ccgattatgc cccagatatt tttaactggc
    19801 ttgatgatat ttagcgggat tagcgtgtta atttttctta taattcattt agccaagacc
    19861 ggacttaatc aatttttaca tctcaatacg atatttctaa tcagtttgct catcatcaca
    19921 atcggtttgg gaacttttcc gataagcgct tccctccttt tcattctttt tatgaccaat
    19981 tttatgttaa ttattttggc acaagatttc caaccgatgt ctgattcagc caatttttca
    20041 attgctttgc cctttcgtct gattgcctgg ttaagtctga ttgggttacc gatttcattg
    20101 gctttcaaca ccagactttt gcttttacag gcttcgttaa cattatcatc aaacaagccg
    20161 attcacggaa cgattataag cgccttattg gttttgaatt atttcctaaa tgtcgtggta
    20221 gtgttcagaa tttttctaaa gtcaggctgg tatgaaccga aacatcgtaa aattgatcgg
    20281 gttacgccgc tcacccattg cataatcgcc ggtattagta ttgtttgcct ttatctattt
    20341 tttaccttgc ctaatttcaa tccactgaca actgaactga ggttttttcc actattctcg
    20401 atccagggca gtcaaccgct cgattacagc cagtatagtt caaactcact gatcatttac
    20461 tctgccattc tgttaattgg cttagttgtc ggatttataa cggcaaaata tttcaaagcc
    20521 gagaaattct ctcgctggct aactgaattt agcctcgatt tccgaaggta tagtattata
    20581 ccggtagcga atttcagtca ggtaattgcg caaaaaatta ggtatttcga tacggtttta
    20641 ttctcaagag tcagccaaag tttaatgcaa acagcccgaa cagtaatcct atttattaat
    20701 cgaattaacg gtatcggcgc tcaacaaata accaaattga aagaagttcc tgaaaagtat
    20761 ttaaatagaa atttagatgg gatctttaac ctgaaatttc aggttttaat cggaatcgtc
    20821 ttaagtatat taataattac cgttttaatc tgtattcttt aagttttttt ccggaggaag
    20881 tgatttatga ctgaacatct attatctatc aatttattga ttgcgctgat cggcttgcta
    20941 ggaattgcac tgacaaaacg cgagaaaggt aatctaataa aaactattgc cgccatcgtt
    21001 accggtctgc aattctggac cataatagaa attttattca attttaatcc gatggatacc
    21061 gccctgcagt tttccgaacg gattaattgg attccagctt tccgaattga ctattatctc
    21121 ggcgttgacg gcattaatct ggtttttcta attgttactt ccctattgat tttcttaagt
    21181 gtgataattt cctggaataa aggagagcca ctaaaagagt attttattct gttaatgatt
    21241 ttggacattg gtttaaacgg aatatttata gcgataaact tgtttctatt tttaattttt
    21301 cttggggtta ctttttttgc tatctatttg ataatagggt ttagtaatat acgaggaagc
    21361 gttaattcgg ttaaccgatt cggaattccc tttctgataa gttatatttt cattttgctc
    21421 ggtgtacttc tcctttataa gagcaataat cttcatactc ttaacctgac cgaattaact
    21481 gtcggcaacg cgatttctca cagcaaacag atggtgataa gtataatttt attaattggt
    21541 tttgccatct tgattccact ttttccattc cattcttggc ttattctatt aattaaaaat
    21601 gggcctaagg aagttagcct attgatctta ggaatagtga ccaaagtcgg cttgtatgga
    21661 ttggtccgta ttatcgcgcc ggtaatgcca ttcgctacca actcattgac cctggcgctt
    21721 ggtatctggg gattggtaaa tataatttac ggagcaattt gcgccctcgg gagcgatgat
    21781 agcaatcaga ttcttggtta ttttactctt caacaattag gattcttttt aatcggtttt
    21841 gctacgctcg gcgaaaactc aaacatttct gtagcagtga cgggtttgag tggtagtttg
    21901 ctaatcgtta tcagtcattc ctgtctggcg actttgctat taatcatttt caaaaatatt
    21961 caagcggaaa accagaacgt tgctcggtca tcactcccaa atattgcgat agtaacgctc
    22021 acgctattgg ctggcattgg aatgccaggt tttttagatt tttttgctcg ctttttaatt
    22081 ctattgagtg cttttcagac tcagagattg ttgatattgg cgattattag tttgctcggc
    22141 atatttttaa acttcgccat ttatatgaaa ctgtttaaaa aactggtttt tagccagcat
    22201 actcgcgata acgcaaaaag gttagattcg aatgaggtat ttgcgtcggt cagtttgctg
    22261 atcgttctaa tattgtgcgg catatttccc agttttctac taaacctcat ccaacctgga
    22321 attactcgcc tgctggaaat gttcagcatg gcaatcgcgt tttagtctag aaatgtcaaa
    22381 ttttcagtct atcatctatt tcataccgga aattattatt attattgcgg cgattggatt
    22441 tttattgctg actacttttt ttaaaatatc tgattctaaa aaatattttc tgatcagtgt
    22501 tagtctaata atttcattaa ttgccaccct tttatcgcat tcagctgacg gacatagtat
    22561 tttcaataat ctgctggtgt gggattcata cggacaattc atcaaaattt ttacgcttat
    22621 aatagcctgc attattctca taatccgccg taaggagaat tgtaatgaag ccagcccgag
    22681 cccggtcacc gagtttggaa ttggactttt gctcctaatt agtgtggcgt ttgtgagcat
    22741 taatgccaat catctcgtcg ctttgttcat atcacttcaa attctgcacc tcgccggttt
    22801 ttttattgtt cagagtcgga atctcagtcc ccggattatc accgccagtt ggcacgattt
    22861 aatgtttgac atcttcacca gcttgataat gttaatggga atagcgctaa tctatgatac
    22921 cacaggtact tttttcattg ctgaaatcgg ccaaattctc agcactgtga ctcgtatgaa
    22981 tttccctttg atattaggaa tagccctgtt attagttgga ttttccttta ctattacagg
    23041 aatttatcgc ttgagctgga aattggagat tttgcggacg atctccgctt ctgataggct
    23101 attgctttta acgattccgc aattcatcga aaccactacc ttgatacgat tactcagagt
    23161 cgggctcgga ttggtcggtg attggcgtcc tctaacttta atgataggaa ttttcgccgt
    23221 tctaaccctt ttgtttccga ttatttggct aatcaaatta cgttcggcga agtatataat
    23281 tatagttact tcaatagctc aaagcggttt cggactgtta gccttatgcg ccggtaatat
    23341 atcgggagat gtagccgcat tgttttacga ggttgtcttg attttcagtt tgatagcatt
    23401 cacgatcgga gaaattttaa cgatcaatgc aaaatccagt cagttaatcg cattagcgag
    23461 cctaatcggt attccattaa ccgccggttt tccagccaaa tatctggttt ttattagtct
    23521 cgttcagcac aatccattct actatttttt aattgtaatc gggatcggta gtgccgcatt
    23581 gatgttaata ggttatgtac gtatagtaag gcaaattatc accgcacaag ggcaattttt
    23641 caattcaccg caaaaaggca gtcagctgat atttttcatc cagattctga ttaacattat
    23701 attaatttca atcggttttt actggacgcc tctgacggat tacctccaat ccataatgac
    23761 tttctgcgtt ccttaatttc gtttgaccaa cattatttta ctgattattt ctattgatcg
    23821 tattatatta aaattgatgt attactagta aaataaggat tactatgtca ccacaaaata
    23881 aacgcttttt tttctggttc gttattgcgt ttctaatcat tttcataatt ttaatatcaa
    23941 tttctttaat ccataatttc aataaatcca agataatcag tttaccagcg caggagatgc
    24001 ggggcgtctg gatgagtcgc ttcgattaca ctgaaccgtt tccctcatac aatcccgatt
    24061 caatgaaaac ttatatcagt gattcctttc gccgattaaa agatgccaat ttcaatgtaa
    24121 tttttttcca ggtacgcggc aacgccgatg ctttttacgc ttcacgttac gaaccctggt
    24181 cgaatctttt gaccggtgaa ttaggacaga atcccggttg ggatccgctg gcttatgcaa
    24241 ttgaaaccgc tcatcagctg ggactcgaat tgcacgcttg gcttaatgtt tttccagctt
    24301 ggcggggaac cgctcctccg accacttctc aaccgctcca tccttatttg gctcatcctg
    24361 actggctgat atgtgatgga aatggtaaaa aaatgcttct ctcagatggc tatgtcagtt
    24421 tttctccggg aatcccggcc gttcataatc atttaataaa agtaatttcg gatatcgtca
    24481 gtcgctatga tattgacgga attcacttcg attacctacg ctatccggaa caatcggaac
    24541 tcaaaggata ttcacacgat ccaattagtt gggctcgttt ccaatcacat gaaggcaatc
    24601 cacttggtct ggactggaca gattggcaaa gggaacagat taacgaattt gtagcccgcg
    24661 cctataactg cgtaaccgac attaaaccgg agataaaagt ctctgcggct gttatcggta
    24721 gctacaagac cgatcattgg aatggttatt cagcgggatt tcaggaccca cggcgttggc
    24781 ttgaaattgg gaaaattgat ttaataattc cgatgacgta ttatcacctt aatcacaaaa
    24841 attttacttt taataaggct ctcaaagaat ggcaatcagt gattgataac ccggatcaaa
    24901 tcttccccgc cttagccgca tacaatctca gttgggaaga aataattgag gaaatccatt
    24961 tagttcgtaa tagccatttc aagggcatgg tttttttcgc cgctagttcg ctcgatagcg
    25021 aaaagctcca atcactgcaa acaactgaat tccaatatcc gtctatcttc ccatgtttga
    25081 cctggaaaga ttcggtcgca ccgccgtccc caattaattt cgcagtggaa aaattaacat
    25141 cagattcatt agaatttatc tggcaaatcc ctcatcaatc caatgatatt aaaaaatttg
    25201 taatctacca gtcaaaaaac ctgccaattg atttaacaaa gggtgaaaat attttggcta
    25261 tcgtagcggg tagcgacact tcctaccaaa cgaagttcaa tctaccagat cagcgaaggt
    25321 attattacac cataaccagt ctcgacgccg cttataacca aagcgaaccg gcttcggtca
    25381 ttaaaatcaa ataacggatt ttatcagtcg gagcattttt atcacatttc cagataaaat
    25441 atgtgaccca gatcatagat tgacaatggc ttcgactctg gtaaatcaga ttttttattg
    25501 caataggaca tatattcctc taaattttac gctaagaaca taaaatatga attactatta
    25561 taacatacga ctgggaatat tttatagcct ctatgctgaa aggattcttg atgaaagtta
    25621 aattctgggg cgtacgcggc tcgattccaa ctcccccaac ttccgaacaa atccgccata
    25681 aatttttaag aataattgac aatttaccaa ccaacattcg gaaaaatcca actgaaatta
    25741 agaaatatat tctcagcttg agctatctag aagccgggct aattggcgga aatacctcct
    25801 gtgtcgaaat acgagccgat aacaaaatat taatatttga tatgggaagt gggctgagag
    25861 cactgggcaa ttatttagta aaaaatgaac aaaataaaaa tggtctggat ttacacattt
    25921 ttatttctca cactcattgg gatcatataa tgggctttcc ctatttcacg cccgcttttt
    25981 tccccaataa taaaattacc ttttactcgc cacatcccga tataaaagaa cgcctcgaaa
    26041 tccaacaaga ttcccgtttc ttcccagtct cgctcgaaca tatgctggcg aaaaaagaat
    26101 tcattcaatt gcaacccaag acttctatta cattaggaaa agtaaaaatt tccaactacc
    26161 cattatatca cccgggcggc tcctatggct accggattaa ttgggatgga aaaagcgttg
    26221 tctatgccac tgattcagaa tataaaaacc tcgaccgtga atctactaaa cactatgttg
    26281 atttctttcg cggggcaaat ttattaatct ttgacgctca atatactttc gaagaagcca
    26341 tccataaaga ggattggggg cacagttccg cccttattgc agttgaattt gcagtcgaag
    26401 ccggggtaaa aaaattggct ctattccatc atgaaccgga aagagatgat tttgaaataa
    26461 atgatattct caaaaaatca cgcgatttca agaaaataaa tttcccaaac gaaatactgg
    26521 acattttcct ggctatggaa gagttggaaa ttactctata actggaatcc tattatttta
    26581 atttttataa aatcttagag ttatcttaac ctgctaattt ataaatccgt ataaagctgt
    26641 tgatacagtc cggttttgtt attttataga tcggaatatg aaaatattgg tgaaattgcg
    26701 aagtaagttg gaaaatatga tcaagaatca gcgttaaaaa ctttgagcat ccggagaaaa
    26761 tagaagatga cacaattcaa ttggtttgtt ttagttatta ggtagaatat taaaatccaa
    26821 caatccacct cattattttt caagcgcgta catttccggt gttacttgat aaattcttag
    26881 cggttcacgg aattcaaagg aattctccat ctccattatt ttttcatcag aagtgagtat
    26941 tttcagcatg ggttcattaa gtttggtttg aatgtctttc ggatttacct gggtagggtc
    27001 gaagtatatc aaaagcgctt cgcgatcagt gtagtcagtt cggactctaa caaccccgtc
    27061 gaaaaaagaa atatgactgg ttaaatattt taatgcccgc cgcacggcaa tgctctcggc
    27121 atcaggcaaa ccgatctcaa aaatcattaa atcagcgggt cggtacttct cgtaactatt
    27181 gaatgcctga tcatatccag cgaaaagctg acgccggaaa gtaacataat caagggtatc
    27241 aacaaaagcg ccccgccctt cacaacggaa attgaccttc acgacctcgg tttttccgtt
    27301 aggcaatttt ttctcgtaag atttttggtt gatcaaataa ataattgagt cgggagttac
    27361 tttgccaagt tcgacatata tcctcgctat gactggttcg ccgaaattag tctcgaaacc
    27421 gacgatttta ggattgacgc gcaacatacg gattaggtcg gcattatcct gaacatccca
    27481 taatccacta attggaattt caaaaacggc gattttttca ggctgatatt cgtcgaattt
    27541 ttggattttg tactgataag gcgtaaaaat ggcttttttg ataccatatt cgtcaatttt
    27601 ggtgctgtca tagtacacaa ctactttatg actttttacg taagtatcca agccaacaat
    27661 tcccggttgc ttcattaatt tattttgaag tgatttggaa ctgccccagc atttgacaga
    27721 ttttaaacct tccatttcga atttctgaac cgattcgagc tcagcataat ttccccaccg
    27781 ctccgagagt gtcttaaact caaagttacg ggctgttaat aaagcaagga taaaaagtac
    27841 cattaaagct actggaggaa gccagccata attccctttg ttaagcctaa tacttccact
    27901 actggagcat tctttggtgc aatctaaaca aagtgtacaa tccggatgag tgactttctc
    27961 gtaactactg accggaatac cttgcgggca gttatcgtcg cagttaccac aagtggtgca
    28021 ggaattttta ttaatattaa ttcggaaagg cgaaattgag aaaaatttaa aacataggat
    28081 ttccgttaat gcgccggaca ggcacaacgc caataataac cacaggatat gcaatttgat
    28141 accaaaaata tagagaccga aataaataat cagaatggga gctgttatca gaacattact
    28201 aaagatatta ctaagagctc ccagaggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28321 nnnnnnngaa tgggagctgt tatcagaaca ttactaaaga tattactaag agctcccaga
    28381 gggcaaatat atttgcacca gaaaaatcga ataaaaacgg atagaactac aaccgtaaaa
    28441 atagtcaaaa taccaaccaa aagtaccgta tcactgccaa atccagttac tgcggcataa
    28501 tagggatcga attttttgca ccacagttcg ctggcggtag cggtaaaata ggctgtgaaa
    28561 aagagcaaaa cgtatttacc gaggcgcaga atccgatccg ctttcccttt cagaacaata
    28621 gaaaatttaa atcgggcaaa aagtttattt aaccactcgg ttactgtgcc aatcgggcaa
    28681 atataaccac aaaataattt gccgattaga ataacaccaa ttaatagaaa aattcccata
    28741 aatacctggg tctcattcat actgcaagac atcgaaccga gatgaaattt actcccgagt
    28801 gaaactaagc caccgaaggg acaataagcc tcgaaatcgg cttttttagc tttatcagtg
    28861 catccgatga tcactaaaat aatgattaat aacaggatta tagcctgaaa caacagtttg
    28921 gttttattat ggaaaaagtt cctgcgcatc taaatgaatt ccttcgttaa ttttctggaa
    28981 atagtctggc aaaagtaagt agcccgcact ctggtaagaa aggactgtcg agacagccct
    29041 ttcttatcat aattgcccaa ttagtttatt tcacaatagt catcttcttc acatttctgt
    29101 aatctccagc ctgtatccgg taaatatacg taccactcgg caagttgctg gcatcaaagt
    29161 ttacgtgata ataaccagct ttcagaaacc cattttgtaa aacggcaacc ggttgtccca
    29221 gcatattgta aaccatcagc gtgacctggg tgctctttgg taaggcaaaa gcgatcaccg
    29281 tagtcggatt aaatgggttg ggataattct gccgaaggct gaattctgtt ggtaagccgg
    29341 aagaaccgtt cgcaacgcca acaaacgata gagcagtcgc tccttcatcc gcacccatat
    29401 aggtgacatt gggtcgcgca tcgccatcaa tgtcggttga caaccaagcg ggacgatgac
    29461 aggctaaatc gttgtcaccg atagaactgc cagtaacgtg taaatcagtt gccgatacga
    29521 aattgaccgg tttattgatt gaattggcat cctgaccgga agcggctctc catgtggcaa
    29581 ggtcggcacg tggtatcaaa ccaaagagtc ctaccgaagc actggaaccg gcaatataga
    29641 tgtcgttata gtcagaagct aaaacactat tgggtgaagc ggtattgtgc caatgaatgc
    29701 cgaaagaatt ggcggcatcg tggcggttgg aaaagatatt gttatagata tttaacttag
    29761 ccgaaaaggg aatttctgtt ccaattgcgg cgtgaacccc ggtttgattg gtgggcgcaa
    29821 tatcaatcgt attgtggtag atattagctt cgccgctcca attcgccgaa ctaaatccaa
    29881 ttccataaac tcgattatca gtggcagagc cgttattagt agtattcatc gcgataaaat
    29941 tattgaaaat atctatgaca ccaccgtttt caccaataac tattccacca gcgaattgag
    30001 gcgcagttgt gttcatttca acattaacta tgcgattatt aaaaaccaca caggtgtcgc
    30061 ccgcggcaaa tccgtttatg tacatgccgc tataaaatgt ttgatcggca agcggattga
    30121 taatagaaat aacattgttg atataggcgt tttttgtgat gtagtcagcc attataccgg
    30181 taccagcggc atagatatta cagcccataa cgacagctcc gcagggagtt tcaggcgaat
    30241 tacccctaag cgccactccg ttggcgacgg ttttttcagg agtcccgatc tggcaatttt
    30301 taattaaaag actattagga acaccggtat atccatctac gcccaagatt gaaccgcttg
    30361 ccagcagatt ttcaaaagtg atattgacgt tcttgacggt gatgaaatcg gaattgtcga
    30421 tcacgcctat tacacacttg ccatcagtat cgttaccaat aattgtcaaa tcgcgagagg
    30481 ttccaccgct ggtattactg ccatcaatag tgacatacgc ggtattttca atagtaaaac
    30541 cttgcccgga gtaattaccg gtagttggga aatcattgat tgtaatggaa ggcgttttgc
    30601 cggaagccgg tttaatcgtc aacgcagttt cggctgtgag atcttcgcga tcgatgatta
    30661 attcagagcc ggattcaatc aagtcggcgt cgatataaaa agtaactggc gctatggcac
    30721 cataggtatt cagtgccgtg acagccgcgc ccagagtagc gaaaccctgt tcgtgatcgc
    30781 cctgagggat gtaataatca cccgccagaa cattagaata gatgacatat ttcaggatgg
    30841 ttccattttg accaactgcc catagaatac ccacactgcc attatcactg tagcggactt
    30901 tttgcagaac ctcatctgtt ggacttgttt ctaccgtcca attcagaccg gcatcttcag
    30961 acttcaaaat agttccgtat tgtccgacaa caaatacggt attgtcatca acaaattcca
    31021 catcatatag ttcagcgcca ttactggcgg tataaaccaa agtaggcata gcatcccagg
    31081 cggttgagcg gtaaatatag ccggaggctc ctggaattat accgatgccg ttgcgaaatt
    31141 cgaccgcccg caagttttta gcttcactgg tcgccccaag tttggtaaag gtcatcaaat
    31201 cagtcgaagt ataatattct ccactagcgc aggttgctac gaccgcattg ttggataatg
    31261 gccagatatc gtaaaccgtt ttggaaccag cggtagcttt ggtccaagtg ttcccaccat
    31321 cgaagctgta acaaatatag ccgcttccac cccctacaaa aaccgtatca gaatttaggt
    31381 aatgcatagc attaacggct ttattgttat tatacatcgg gttatcaatc tgagtaaaag
    31441 tagcgccacc gtcagtggtt ttcaaaatgt atccaccact gccacctata agtccgttat
    31501 tagcgtcaat aaattcacat tcaaaaatat cgccgtcgag accagtcctc agattgttcg
    31561 gataagacca ggtttttccc cagtcgttgc taaaggtcat ttcaccacca ccgtctgcag
    31621 ttaatacgat ttttttctcg tccgggaaag ccaatcccaa ccagctgatt ccaggccaat
    31681 tattaatagg aatccaattc accccaccat cagcagtttt catccattgc tggtattcgc
    31741 aaaaccagta accggtacgg gcatcaataa aatcagccga gagaagcctt tgacctgttc
    31801 cagtactaaa attagtccag tccgttccac cgttggtcgt tatagcacca aaattatcag
    31861 agccaccgcc ataaataatg gtattattat cgaatacctc aacataagag gtatagacat
    31921 tggcaaattg aacaacagct ttgttttcaa aagtcctgcc gccattagta gataccagac
    31981 agacccgatg atagcaagct agggctacgg ttgaatcgcc tccgacggct acgtcataga
    32041 cccgatagga agttgcgccg gttgggaaag ccgcagcaat tttcaccgga ttccagtttg
    32101 tgccaccgtc ctcagtccac cagatagtcg cttctttagt aaaatccacg gcaactactc
    32161 cgcggttttc atcgaaaaag gcaataccac catcaagatc atcgcgggtt tcatcaatgg
    32221 cggtcatatc gccatcgaaa ttgtaggaaa gtttattgaa agtagagccg ttagtagttt
    32281 ttagaaccgt accgctttta ccagcgcaat agaccacatt ggcattaacc acatcgagac
    32341 tatacagatc gaatttatag gtattggtat cgccgatcat agtccaatcc aagccaccat
    32401 tagtggtctt gtagattata ccaccattgc cgcaggcata gccgatgttg gcatcaataa
    32461 actccatatc attccaggaa ttggcggata catccggctc acgcacagtc gtccagttag
    32521 caccaccatc agtagttttt tgcaccttgc ccatttgttt gcttggatag gttgtactat
    32581 atactatcca gccggtatta gcatcaataa aagacacctt gttaacggac atatccgtcg
    32641 aggcaatttt tacggtctgc caagaaccgc ctaagagtag gttcgcaaca gttagcatca
    32701 gagctgtgac ttttaatact ttcttcattt tctctctcct ttttaaaatt ttatttcttt
    32761 atacccatac tttaaaatat aagtaattag caaaatcacc gtgctcataa tttattactt
    32821 ccaataaaat atttttagac caacggattg attgtatgga tcgggattgt caaaaacggt
    32881 ttggaaatat tccagtccag ctccaaaccg gccgaaagtt gttagacgtt caattccaat
    32941 ttcgcaccca tagcgagttg cccgctcagc attccaataa aaatacaaat catcaatcgc
    33001 cagatttccc agaaaataga ctgacaaaat ctgattgatg gcatagttta aatcgaggaa
    33061 ggcagatgaa tgatagtcgg tctcgtggta ttgaaatgga atagtgtatt cttgatagtc
    33121 acaagcttga ctggcgtaag aggcgccgat attcattttc agccgcttaa aaagccgagt
    33181 ctgccagcgc aaagaaaaga cattgagcaa ttcggcatta tccagaataa tgacctcata
    33241 attgccagat ttcacccagt cgtccgtaga gcggatttca tagataaatt ccagggaatt
    33301 attcgattct ataggggaat aattcagatc aaggattccg cgccggattt cacgtaccca
    33361 atgaccacgg ggcgtgggtt tctggggact tccaacggtg acattatccg aacgtccgtt
    33421 atattctccg gtaattatca aagccaggcc aggaagcata aaatcacggt ggcagaattg
    33481 caaagccagc ccgccgccct cacgttcatc attttttcta ttaatggatt tcggagtttc
    33541 atccttataa atatgataac caaacagagt gcgtacgaag gcatcttgat agtacttcac
    33601 agcttcatat ttgcgctgag cgttataata ggtccccaaa agccccccga ttattttctg
    33661 attcttcgaa atataactca agccgccgcg caattcagta ttccgggcga taatctcgca
    33721 ttgggtatag acatccttca aactttgttc aactccgtaa ttaatctcaa gcccgagatg
    33781 caggcgtttg gttaaggcat agtcataaag aaaattaatt tgtgggctat tataagttgt
    33841 cgtgccagtg gtcgtatcca gaaaagcaat atattcgtca taaatatttt tttccaaaga
    33901 gtggtagaga tcacgctgaa tagtccgatt aaacctgata ccggtaacca gggtgttgcg
    33961 ctcactgaga tgtttggcgg aatgaaaatt aagggcataa tcttcctgac gccgcgggtc
    34021 atatagccgt ctaaagacat tattatcatt ggtagcttgc atctgataga aatcgagatt
    34081 atccgggtaa gaaatgccgt agaaggcggg attgccggat aggtcataaa tagtgagatt
    34141 atactggttc attaaattac gcggctgaat ctcacgccca agcggattgt caatagcgaa
    34201 taatgtagta atgaatacca gtccaatcac tactcctaca aataggcatt tttttataaa
    34261 atcactattc gttctcatgg tgatgggtga tgatatcctg gggtaggcgg tttaataatt
    34321 acgaagtcgt taaaggaatt attggtatca aagccaggca gaatccgctc aaccgactgc
    34381 ccgctatacc tctgattgcc aacgccagcc caaccggcgt ctaaaatttt atcaatgttt
    34441 ttggttgtct gttcgctagg gcttttactg ttgtattcca cgcaatccac tacggtaccg
    34501 atatcgagtc cgtcggtcat aaccgagtcg ctaccatcgg cgagtgcgat gacatttgtt
    34561 gatagattga tcataaaatc tacggttcta cccggtacga tattagctaa atttggaaca
    34621 ttaagattat cgatgtcgcc gaaatccata gcattgacaa attcccaatc ggctccagaa
    34681 agatcaacgc tggtgctgta taactccagc caattaactg catcctgggc aaggacgaca
    34741 aattcacctg gttgaaccgg atattctcgg ccggtcagcg gtgtgccagg aaagcggaaa
    34801 gcataggtta cagacgtcaa catatgataa atccggcaga cgatcatccc atccagatat
    34861 tgaacctcat ccgaggaatt atatagctcg ataaattgat cgtaaaacca attattccga
    34921 ttttcggggc cagtgtaata aatctcattg attttcaagc caggccggag gttagcggta
    34981 atggtataaa ttgtatcggt gcctattccg gtttcggagg gagtaatcgc cacaattgtg
    35041 tcaatcagag tatagcttat gagcgaatcc agtcttctat tcccaaaact atcaatgaac
    35101 ggcaactcga ctttggcttt cacctgcgga tcataaatcg cccaggacaa attttcaaaa
    35161 accactaccc ccgaatcatt agtagttgcg caataatcat caaggttata gtcgccagta
    35221 tgaattttga catcgacatt tgatagtctc tcatcgtaac cgagggtaga gtcccgatat
    35281 accaatacca gttttaaagt ataaatgcca tcgaattccg ttggtttctg gtgaaaacag
    35341 ttcgttaaat tcaagagtaa gataacggtt aaaagtgcag ttttttttac cattgagcac
    35401 tcacttccaa tccgtaaaaa atcggcggat tgtgttcgtt taacgaacca tcgaaatttt
    35461 tccatacagc ccggtcatcg aatatattat tcacaaacaa ggaaatttcc atactttgag
    35521 agagcgactt ggtaactcgg aaattgaaga gccaccgacc gccggtatcc cgcagataac
    35581 gctcataata ataggtcatc ccctgatacc acaagtaagt cacgccattt tcagtcttat
    35641 aagtcgaatt gctgcggtaa atacgcttga aatgttcgag aggcacttgt tgagcatcga
    35701 gtgtaaccca gatgccgagc cgattgctga tataattaat ctgatagtct atgatgattt
    35761 tttcactcca gtatgactcc ggcttgtacc aaatatcttc ccaattttcc gggttagcat
    35821 catagaccaa gccggttttg ccgtttttat tgaaccgata ggtgacattt aaccggtatt
    35881 ggaaattgtg aatgcgctgg gtgcgcatcg taaattcgat cccgttcgaa cgactccagc
    35941 ctcgattttc gaaacgctcc caagccgcgg cggtaatcgt atccggatta atttcatagc
    36001 cataggggta actgaccgta accggcatat tgtccgaggc ttgataatag ccagtcaggg
    36061 aaaaacctat cagatcgctg aatttccaat cgagagaagc ctcatattta tcaatcgaat
    36121 aagcctgtaa atccggatta tactgcaggt gcacttcttc tatgatcgta tcacctgagg
    36181 catatttgaa ataatctgga gctttaaaaa tgtgccctaa ggaaatatct ttaacgcttc
    36241 ggccggcgcc taagcgcatc tgccaatctt cagtgattga gtactgcaaa ttcagacggg
    36301 gacttagaaa atctccgtgc tctgatttga gaaaggcttt atccttgaac atatttttga
    36361 aattgaatcc aatcggagag taagcgtcat agcgtaaacc agccattagt ttatagcgcc
    36421 gcgcgagcat tttgccctcg aggacatcct cggcgtagaa actcagggac gtcaaatcgg
    36481 gataatcatc gaaagcatag gagcgatgcg aggaatattg cccgtaatag gggaaatttt
    36541 cgtcaattac tacgccgtcc ccggtgtttt tttcataatt tacttccccg ccgaataaaa
    36601 gattatgctt tgtatgcttc gttgtcaagg cgattttacg ttggattttt ccggcaattt
    36661 tccattcttc gccgatttct ttcatcttgc cgataaatcc cggcaaaatt ttaatcaggg
    36721 ttgtatcaat cgcgattccc gtgaccgaat ccacgacagt tgtatcatat ttaagaatat
    36781 aattttccgc cacccatttg gatttatagt ttcttttatt attaagatta accgagaaga
    36841 cgcctttcca acttttagcc tcgctatctt tataaataaa attgaaatcg ccggttgcca
    36901 gaaatccttt atcataagaa gccatctgat aaacgtcagt gggtttttca ttatcgagca
    36961 acttggtaaa acttcccccg atatgggttt cgagtttttg atctaagtat gcaccggata
    37021 ggttaccgct ggcgtgtaaa cgctgaaatt catcgccaac tttgcgcaaa tcgcgttcgc
    37081 tatacccgta attaaggtga taatccagaa ccgagcgacc gagcttaatg ccatgtgaaa
    37141 agctagcgtt ctttgtatcc ggattgattt tagcattgaa tttaggagca attcgaccga
    37201 tcttggtttc aactttaata acgccgctgg caaaattgcc gtattcaacc gaggggatgc
    37261 cggtaattac ttcgaccgat ttaatattat cagccggaat gatacgcaag tccaggcctc
    37321 tggtggcact cccgtaatag acgttgccgg aagtgttagc gtcggttgag atttgattat
    37381 cattaataat tattgtagaa ccaaaagact cgacggcact cattgataca ccagcggcgg
    37441 tcaccgaacg caagccgatt tcactgcggc ccgagagacc aggattctgc gatttgtcca
    37501 cgccgggaac aagtgagaga atatcgccga ggctggtagc ttgcatatgt tcgatttcac
    37561 cagtattgat agtgcgggtg gtaaccggcg actcaggaat taattctttt tgcactgaat
    37621 agacttcgat accgcccagc atgaaacaat aatcgctgga ggatttgccc gcttgcttga
    37681 gcagtttgat attaatttcg taatttcggt cggcttcgat ttttagatca aataacacat
    37741 ttttgaaata tccctcgcgc acagcttcga tattataaac acctggttca acatttgtga
    37801 gcgtgaattg accggtctta tcggaaaccg ttgtacgtga aaaatccgga tcagaacgtt
    37861 tttgcaggat aattaacgca tccggaatcg gtttcctctc catctggcaa gtgactttcc
    37921 ccgttatagt tgaggcaatt ccccgaatcg ctaaaccagt caggattaga attattatca
    37981 cctgcttttt catattcttt tagcgattca tttgaattcg tttagggcgt cgggcgccat
    38041 atccccagaa actgcgatga tgtaattttg agtatcgaga tacttattgg ctacccgctg
    38101 tatatcagct tttgtaacgg caagaatgcg ctctttacta tgatcaaagt aatcaagcgg
    38161 cttcttctcc tgaattaaat cgaaaatgac cgcaccaatt tcgtccggtg tccgagtatt
    38221 catcgttagc atagcaattc ggcgggcttg ggctttttcc agttcttcgt cagtaactcc
    38281 ctccttttga accttctcaa tttcagcgaa aatgccatga atcattttac taagattggc
    38341 ttggtctaat tcagtacgaa tgttccaata accgccgtga tcacgaatcc agagattgct
    38401 tttgatacta taactcaagc cttgtttatc gcgtaattct tttcccatgc gggaggttag
    38461 cgaacttccg caaaggattg attcgagtag tttaagagct tcaatatcgg gattatctgt
    38521 tttgatgcca ccttctactg ggcaaaaggc gatatctact cgacattgtt tgtatcccgg
    38581 attgctaaaa acatagacgg ttttaccctg gatcggctta accgtctgga acggcaaatg
    38641 cttatctccg gatttatgtt tccaactgcc ataagtggca tttaagcgtg aaagcatttc
    38701 tttgcgattg aaatcgccaa ctaccaataa tttcaggttg tcaggtgcat aatatttggc
    38761 gtgaaatttt tttaaatcag cactggtcaa aattctgtag gtttccggat ctccggcgta
    38821 gggtaaagca tactggtgcc cttcaaaaac tttttcgaac atagcgtaaa aggctttcat
    38881 acgatcggtt tctttatatt ccttcgcggt tttaattaag gacgggcgaa ctttttcaat
    38941 ttccgattcc ggaaaagtag gctgagttac aatctcgaaa ttagcctgta gaattttatc
    39001 tacatctttt gagagcccat aaccctgaaa actaatctta ttccagctct gactaatatc
    39061 aatctggtag ggtatgaacg agcgctcttc gatcaattct tcataagtgc gtttagcggt
    39121 gccccgcgaa agcataccta ccgtcatctg gcgaatacca ggatgcaaat tttcctcttc
    39181 gagccgccct gtttcgatat atccgattag caaaatagtt ggatattcat gattttctaa
    39241 aaaataaacc ggcacgccgt tttttagctt agctgtggta accaacggtg cgatcggatg
    39301 cggtgaaatc acttcatcta atttaggaaa atagatcttg cgcacttccg gattgacata
    39361 tttatcggtt gtatgttcgg attcttctat cgtttccggt tcggttattt ccagatcaaa
    39421 ttcactttcg tcactttctg tagctaagaa tccgactttc ttttgggtag tatctgggtg
    39481 agagtaacag gcaacataat tgccggtttt caaatattta ttaacgaccc gtataatgtc
    39541 atcagcggtt accgccatga cgagctgatt ccattgattt atataacgcc aattataaaa
    39601 attatcataa gttgcaagtt gcccaccgat tttttcgtta tgctgattgc cagttagttc
    39661 gttaaaggtg gtattatttt taatcttctg gagatcatat tccaacaccg ccgtcgtcgc
    39721 caaactgtca atttccttcc agattagatt ctcaacttcg ctgatttggg cagttggcat
    39781 caagctggtg attagattaa aagtcgaggg gtcttttgta aatgaaaggc cggctaccgc
    39841 cgattcacaa acctgctttt cgcgtttcag ctttttatta agtcgcgaag tcgaactgcg
    39901 ggcgcccaga atctgcccgg ctacgtataa agctggtcca tcagcttcaa aacgggtagg
    39961 cgcttcaaaa taataatgga ttgattcgct taaaatatcg ggatgaatga aatcgagcgt
    40021 tctcttgccg caaattggaa cacgataaaa attatacgcg ggaatctccg gaccgcgcgg
    40081 aattttacca aaataggctt cgactttttg cataatctcg tccgtattga agtcaccggt
    40141 gagaacaagc gtggcattgt tgggagtata ataggtgcga tagtatttat aggcgtcatc
    40201 acgattaata ctacgaatat cttccatcca gccgataatc ggattgcgat aagggtgatt
    40261 tttgtagatg agagtattaa tctcctctcg acgcaatcca accgcgctat tctcgatgcg
    40321 ttgtttgcgt tcttccatta taaccttgag ctccgattcg aattcttgag ggtcaaaagc
    40381 acatttatac atccgttcgg actcgatgtc gaaggctaaa tcgattttat tttggggaag
    40441 taattcataa taaaccgtga tgtctgtgga ggtataagca ttgaatatac cgccattagc
    40501 actaatcaat tcggaaatag tacctttcgg gaaacgatta gtgcctttaa acatcatatg
    40561 ctcgacgacg tgacttatcc cggtctggcc aaacggttca tatttcgatc cgacattgta
    40621 agtcaattgg gaataaatga ctggtgcggt gtgcatttca tacgtgatca ctttcagtcc
    40681 gttatttaga gtaaattcct tgacccgtga ttcatcgaga gccaggagac tcgagaaaat
    40741 aattaatatt attgcaattg tataggctaa actacgaaac catttcattc taaattgttc
    40801 cttttttgat ttaaatgttc actaatattc taaaaaactc aacgaatata aaataacttc
    40861 taaataaaat caatgaagtc tataaagact cggggaaaat gatttgctat atttcttaga
    40921 gaaaaattga tagccagaat tcagcaatag attgttataa gggcaaaata aaatcggaaa
    40981 ttagtggata aagaattgat tcaattctgg tatgaaacac taaaaaatat ggaaactatg
    41041 agtgtccccc taaaatttta attgtttcct ttgtcagata attatcttgg caaaaagata
    41101 atatttatct aaatttccgt ctcttttgaa attagatgtt ggctgtttga aataataagt
    41161 gagtctgttg tttttccagc atatccggga gctgaagcgt aatcggcttg tcggggacgc
    41221 tcgactgtgt atggaatctt tgccagcaga gatagtggcc gccgcagtat aatgtcggta
    41281 aattaccaca cacagatcat catacccttc ccacttgaaa aacaacctaa acaattcggg
    41341 gatcgtccaa tggcaggacc acggcctttg gagtcgttaa tctaggttcg aatcctggtc
    41401 cccgagcaag tttttaaaat gagcctgaat atgaaaaata atggaattat tttttgtgga
    41461 aggtccaatc cccagttggg ataccggatc aatgaaattc tcgggataaa acccggagca
    41521 attaaaatta gtaattttag cgacggcgag atcaccgtca aattggaaga caatatccgc
    41581 ggaaaagatg tcttcattat tcaatctacc aatccaccgg ctgaaaatat tttagaactt
    41641 cttttgatta ttgacgctgc ccggcgcgct tctgcccgta gaattaccgc cgttctcccc
    41701 tattatggtt acgcccggca ggatcgcaag gaccagccgc gggtgccgat ttccgcccgt
    41761 ttaattttag atacgatcgt cgccgccgga gccaaccgga ttatggctat ggatttacat
    41821 tcgacccaga ttcaaggctt cgttaatatt ccctttgatc acctgtatgc caagcccatt
    41881 ttcgtcgagc ccttgcggaa gctgatcggc aaacgcgatt tctcgatcgt tacccccgat
    41941 gtgggcggaa tcaaagttgc tcgttcttac gctaaaatcc tcggtttgcc tataaccatt
    42001 attgataagc gccgcccgaa gccgaatatg tcggaggtca tgaacgtgat cggtgaaaaa
    42061 gatatgaaac atgcgttaat aattgacgat atgattgata ccggaggcag tgttactgaa
    42121 gccgccgctc tgttaaaaga gtatggtacg gaaacaatca ctgccgtcgc cactcatggt
    42181 cttttttccg gcgattgtgt taaacgcctg agcgactcgc ctattgatcg cgtcatcgtc
    42241 accgattcag tagcgattcc ggaagaaaag cattttgcga aattgcaaat tctctcagcc
    42301 gcgccgatgt tggcggaggc aatcaataga attcataatg aagaatcaat tagttcccta
    42361 tttgaattat aagtaaagga gtttttagat ggttgactat tacaaattga atgccgaatt
    42421 acgttccgat ttccagaaag gagtcaccaa gcgcatgcgt cgcgcaggtc tggttccggc
    42481 agtttattac catcataccg aaaagccggt agcattatct cttgatttga aagaccttaa
    42541 ggcggctcta cgctccagcg cccgtattta tgaccttaat attaataaaa aaagtcacaa
    42601 atgcattata cgcgatgttc agttcgaccc gcttaccgaa gagattatcc acgccgactt
    42661 tatgggcgtc agcctggaag aaatggtgac cgtcagcatc ccaattaatg tcaccggtac
    42721 cgccattggc gtaaaaactt ttggtggaat tctggagcaa catctttggg aattggaggt
    42781 gagatgtaag gcatcccaga ttcccgatcg cgttacgata gatgtgacta atttaaacct
    42841 cggcgattca atcaacgccg gtagcctcaa tatcgagggc gtagaaattc taatacccgc
    42901 tacgacttcg atcgtctcag ttgttaaacc gacaggtacc aaagccgaag aagaagtcgc
    42961 taaagaagaa gtcaccgaag aagtcggcgc cgaagaaacg gctggcactc aggaagcgaa
    43021 agaaactaaa gagaccaaag gtaaatctga gaaataatga acagctcaat gaacggctac
    43081 aaatgatcag gacaatcatc ggtttgggaa atcccgggaa aaaatatcag cacaatcggc
    43141 ataattttgg tcaaatgtgt ctaaaccaca ttgccagacg gcttaagctg gcatttaaac
    43201 caggaggcgg cgaccgtgcc tttacttaca ctacgactgt aattgctgat cggacagtct
    43261 atctttgcca gaacaccact tttatgaata atagcggatt agctgttcgt cagattctga
    43321 accaattcga cctgcagact gatgaaatac tggtagttta cgacgatatt gacttgccac
    43381 tcggtaaaat ccgtttgcgc gagaagagct ccgccggagg gcatcgcggt atacgctcta
    43441 taatcgagac gctcgccacc aaccagtttg cccgcttgcg gctgggaatt ggtccgcaag
    43501 atgaaggcgt tccatcggaa gattttgttc tgacggattt tcgtccagcg gaaaaagaac
    43561 tggttgaagc cgtactggaa gaaagctata agtgtatcct gaccattcta aacaatggca
    43621 ttcgaccggc gatggaatat ttcaatcgcc gcgctctgca ccttgctccc aaagaaaatg
    43681 aaattcaggt aaattcaatt aaaaaaccaa tcatattaac ataaggagtt cggacttgag
    43741 gtactatgaa aatttattta tcgtaaatcc caatatcgaa caggaaaaat tcacgcaatt
    43801 gattcagact gtcaaagccg aaatcgagcg tttagatggt aacattttaa atctggaaga
    43861 ttggggcaag cgtcgtctgg cttatccaat ccagaaacat cgttatggct cgtatgtgct
    43921 ggctcaattt gaaaccgaaa acgtagaact ggtcaaggag ctggaaagct ggctacgttt
    43981 gaatcagggc attctggctt ttatgacagt cgcgctggat gaaaagccga gtcctaagac
    44041 tactcagcca accgaccagc ccgtggacga aactattgga tgaacgaaga ttggatgatt
    44101 taaaaaagaa aggaaaaaaa tatggtatta ttgagcaaac gcaaaatttg caaattttgt
    44161 gagaatcccg aggaaaagat cgactataag aattacaagg tattgagaag gttcataacc
    44221 gaacagggga aaattattcc cagccggatc accggcacct gtgctcgcca tcagcgcgaa
    44281 ttgacgaaag cgattaaacg agcgcgcaat atcgctttac tgccttatac ctacgacgta
    44341 acccaaaatt ttaaatagta tcagcaacag gaggtattcg tgcgcgttat tttaatcaaa
    44401 gatcatgaaa acttgggcaa gattggggat accgttgaag taaaagacgg ctacgcccgc
    44461 aattacctca ttccgcaagg catcgcgctg gttgctaata gcgccggaat gaaacaaatg
    44521 caggaacaaa tccgcctaaa ggaactgcgc cataataagg ctcttaagaa agcccacgag
    44581 ttggcggaaa aactcaacac cctgtcgcta acgatccccg ttcaggttgg agaagaagat
    44641 cgcgtcttcg gcgccgtgac ttcccagctg attgccgaac agttgcagga aaagggctat
    44701 gaaattgaca agagaaatat acaactcgaa gagccgatca aagccctcgg cattttcgat
    44761 attcccataa aaatcttcca ggatgtgacc gccaaggtca aactctgggt gatcaaagcc
    44821 tgatctattt ttcaaatatt tattaatcca aagaccgaat cgttccggtt taatcttgtc
    44881 ttttttattt cttttggata atctttatag cgaaaattat gtctgccaaa gttcaatatg
    44941 tagatgtcat ttttccggcg actctcgaga agagttttac ctaccaggcg ccgagcggtt
    45001 atgacggaac acttgctccc ggtcagcggg tagtagcgcc gctaaaatcc agctcggcga
    45061 tcggttttat aattaatctc gacccgatcg taccagccaa tttgaagatt cgcgagattc
    45121 aggaaatcat tgatccccaa ccgatttttc ctccggaatt gctgaccttt ttgaaaaaaa
    45181 tagcggacta ttacctcact ccctttggcc gggttttagc cgcagctatt cccgcggaat
    45241 atcgctttat taagacccgc actttaaaag caagtcgtct tgacggtgcg caaccgactg
    45301 aatatggcga tctttttgct tatatcgccg aacggcgtga ggtaaaatac agccagctta
    45361 aacgtcaatt tgagacgact tacctgcaga aaggactgat ggcgttaaaa aaagaaggtt
    45421 ttatcgccga gctggtggaa tttaaaccgt caaaatacca gggacatatc gaaaagaccg
    45481 taagattatt gaacactgac gaagaaacct tgagtgctat tgcgcgcaaa gccccccgac
    45541 aagcggaaat cctgaatgct ttacagagcg tcggggagcc actgaaaggc agagatttgc
    45601 ggcgtttttc accggtagcc ctcaatgctt tagcccgcaa agaactaatt gcgattgata
    45661 caaccgaccg aaccctttct gatttatgga gtgaatttgg tgaacggcgc aagacgatta
    45721 ctcttactga agatcagtat caagtttttc aggcaatttc cgattcgatc aaaagcgcta
    45781 aatataacgc ctttttactc gaaggcgtta ccggtagcgg caagaccgaa atctacctgg
    45841 aggcaatccg tgaaacttta gcacagggtc gtacggcgct cgttttggtg ccagaaataa
    45901 ctttgacaac gcaccttgcc gcccgttttc gtggggaatt tcaggatcag attgccatct
    45961 ggcatagccg actgacccaa gcccagcgca gtgctatctg gggtgcggtt ttgcgcggtg
    46021 aatttccggt agttattggc gcgcgcagtg ccatttttat gcctctgccc aatctcggat
    46081 tgattatcgt cgatgaagag caggataatt cgtttaagca aaaaagtccc gaaccgcgct
    46141 atcatgcccg agatgcggct ttgttgcgcg gtgtcgaaag ccaggcgacc gtcgtgctag
    46201 gttcagctac tcccagcctc gaaagtcagt ataatgccat tatcggtaaa ttgaataagc
    46261 tggctttgcc ccgtcgttat tccaaggctc cgactgctgt aatccaggta gttgatatga
    46321 aagcggaaat tcaagaaacc ggtgatgttc agaatccatt ttcacgtttg ttgactgcca
    46381 agattaaaga ccgcctagaa aacggtcaac aggtgcttct cctccagaat cggcggggtt
    46441 actcgccggt cttgttatgt ccggactgcg gctgggttcc ggaatgtcgc aattgcgata
    46501 tcgctatgac ctatcataaa aaatccgcga cgttaatttg ccactactgt aatttacatg
    46561 aaacaccacc ggcggtttgt ccgcgctgta gaagtagtaa ttttctctac ccgggagtag
    46621 ggactcaaaa ggttgaatat tatttaaaga cggcatttcc tgattgccgt tatgaacggt
    46681 tggatttgga tgctagtcga attagggagc gagcgccccg tgttttaaag caattcgagg
    46741 aaggccaaat tcagctttta atcggaactc agatgattgc caaagggctg gattttccca
    46801 atgtgactct ggtaggagtc attaatgccg atcttggtct ttacctacca gatttccgag
    46861 cccgcgaaag agtttttcaa ttgctttatc aagtttcggg gcgcgccggg cggggtgata
    46921 ttcaaggtga aataataatc cagacctaca atccgcagga ttttacgatt cggtgtgcgc
    46981 tacagcagaa tttagccggt ttttccaatt ttgaactaaa tgaacgcaat ccgctcaatt
    47041 atccgccttt tagccgaatg gcgctcatcc agatttccga tttgaataat caacgcgccg
    47101 ctacgacagc ggccgcggtt gccgatttcc tgaatgccca tcgtggtaaa attgaattac
    47161 taggaccggc ggtggcgcct ttagcccgtt tgttaaatcg ttaccgctat caaataatct
    47221 tgaaatcacg acgtgaaaaa gaccctaatg ggagaatcct gcgccagcta ttgagcaatt
    47281 ttttgaattc ccgggagtat cgagacctga gtcgtcaagc acggatcata atcgatatag
    47341 atccgctcga tctactatga cgccgcaccg catttttatc gtattgataa ttacttacca
    47401 aatccttatg gctacacctc cgataaaaat tgttcatccc gacggttatg aaaagctagc
    47461 ggaatttacg catacttcaa ttttgaatgc attgccgaat gtgcctgcca tctggcagat
    47521 gacgactgat acgattcgca tccaaatcgt agcgcgctca gccgatttcg aggaagctgt
    47581 cggtagcgat ttaccccagt gggtcggggc ggtcactctc ttcccacagg acttgatggt
    47641 agtccgatcg cccgacctga gtcgtagtac tttacaggaa tatcgcagga ctgtcgtcca
    47701 cgaacttatt cacttattgc agggacaatt tgtgccgttg aatctgacgc cggtttggtt
    47761 taatgaaggt ctagcgcttt attatgccgg ggagttcgat ttgcgcgaac aagttgtcat
    47821 ttctaaagcc ttaattcgaa accagttgat tccaccgata aaactggaac aaattttgaa
    47881 attcagtcaa acgcaggctg atttagctta tgccgaaagc gcttcgctaa ttgaatttat
    47941 ggaaaatgtt tatggcaatg gaacagccgt ggcaattttg gcaagaatgc gtaacggtga
    48001 aaatttcgaa caagctgtcc taaaattaac cgattgcgaa tatgacgatt tgatgaacca
    48061 gtggcaaact tatgttgaaa aaaagtannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48181 nnnnnnnatt gcgaatatga cgatttgatg aaccagtggc aaacttatgt tgaaaaaaag
    48241 tacaattgga tatttcttct ggacattcag aatattttgt ggttaattat accgttgata
    48301 gcgtttttgg cttattggag tattcggcgg cggaacaaag tgactctcga gcagtggaat
    48361 gatgaagaaa tcaatgaaaa tagcgatgag caggaaattt aggttaatat tactcagaat
    48421 ggcattagtg agcgccgcgt cggcgcaagc cctttatcag ggactttatc agccgattga
    48481 tgcccgcggc tggggtatgg gttcagcaat ggttgcccag acgcgcaatt cgtccggcgt
    48541 ggcatataat ccggcgattc tggcggttgt tccgaacaaa tggcaggtga attatacccg
    48601 ctacgtattg gacattcaat caaccaacgc tctatttgtc tggcaggcgc caggaaaagg
    48661 taaattagcg gcattgggcg gctatctgga ctacggcagt tttagtgagc gtgatgatga
    48721 cggaaatgaa ctcggcgaat ttcaggttag cgatttaaat ctgcgactag cttatggagt
    48781 cccaatcacc cgcaaactca gcgccgggct gacggcaatc ttcgtgaatt ccgacttagc
    48841 gaattacaat tcaaaggcgt tattaggttc agcgggttta ttgtattatg atccggtttc
    48901 gacgttttca gttggactgg cttatacgaa ttttgggaaa atgctttcag gatatatcaa
    48961 cgatgatgag aaaattcatt ccgcgctaat ggctggcatt gccaagaagc ttgaacattt
    49021 gccaatggtg atcgcggctg actttatgca ttattcatct ggagaaaata tcatcaagat
    49081 cggcggagaa ttcattctcg gacaaaatta tttcttgcgc tgggggacat cgagccggcg
    49141 ttttgaaatc aacactcagc agaccatcac taattttttc agttcttctt cattaggcgg
    49201 cggcttggta attaaccgtt tacagatcga cctgacttgg tacagtctcg gacacaccgg
    49261 cagtgttttt gcttttagca ttgcccagaa cctataaaga ggtgcattat gcgaaaaacg
    49321 atactaattt taagcacgat gacatgtgtc gccttagtgg ggcttggact aatcggatgt
    49381 gaaggtgatg aagccaatca acccagctac attttagaca ttgatcccat cttcggaagc
    49441 gctaaatata actgtaaagg atgccacact ggcgcaattt cgccttatct cgatctgacg
    49501 acctacgaag gtttaatggc cggcagtgat aatggcaagg tggtgatacc gggtgacgct
    49561 gaccatagtt ttttatacga aaaaatatcc cagccaatcc cttccagagg cgaacgtatg
    49621 ccgctcggcg gtccttttta tttaacctcg ggggaaatca gattaatcga agattggatc
    49681 aacctgggcg ctaaggataa ttaagccgag ttagatagat tagccgaata caacatttac
    49741 taatggaatt ataacggtag gtctatcctg aacgaaattt ttataagaag tactcagcca
    49801 aaaaatatta aatagtcgct aaaatagatc aaactttcct tttgggggtt tgattttact
    49861 aaattattat tccgaacatt aacagccaca tcggctactc ataatagaat ataaagtcat
    49921 aggctaaaat ttcgtcattg gaaaa
//
LOCUS       DAOSMP010000014        46696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_835_length_46696_cov_20.987586, whole genome shotgun sequence.
ACCESSION   DAOSMP010000014 DAOSMP010000000
VERSION     DAOSMP010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 46696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46696
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_835_length_46696_cov_20.987586"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PKX09_03980"
     CDS             complement(<1..749)
                     /locus_tag="PKX09_03980"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOU17012.1"
                     /translation="MKIYKWVFSIGLILIACGLIYFSIVNIWSWPVIVLLILGFGASV
                     LALIKLDIRTLLKNRRLIYGGNMVLVIILVIAIVGLVDFFFTRHSWRVDTTATKIFSL
                     SDQTRKILKNLDKDIEIVVFAKKSNKSYINDRLNEYRHYSKRISWEVVDPDEKPALAK
                     QYNVKNYGNLVIICEDKAELVPNFSEEAIINAIIKVTREGKKKVAFLTGHGEGTIAST
                     ERDGFERAKQAIEQQNYAVEELMLVDKDSIPA"
     gene            complement(753..1520)
                     /locus_tag="PKX09_03985"
     CDS             complement(753..1520)
                     /locus_tag="PKX09_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU17013.1"
                     /translation="MSNLLAIYKRDLKGYFFSPIAYVVIGLFLLVNGIFFYVLLSSFL
                     EYSYAVMIQRQGYPINVNLLMIRPFFQNMSVIVLFVIPMITMRSFSEEKKSGTMELLL
                     TSPLTNWQLILGKFLASFTLYFVMVMVTFLFMVFLFIWGNPSLVPILISYLGILLMGL
                     VLVALGNFISSLTENQIVAAVGTFGATMFLWVIGFAGDFAGKVFGEIFKYLSIVNHFD
                     DFSKGVFDTNHLVYYLSLTFLMLFFTYQSIESSKWRG"
     gene            complement(1522..2469)
                     /locus_tag="PKX09_03990"
     CDS             complement(1522..2469)
                     /locus_tag="PKX09_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU17014.1"
                     /translation="MIEVQNLVKHYGSVKAVDNISFTVEKGEILGFLGPNAAGKTTTM
                     RIITGYMPASAGTVKVAGYDVFEQGLEVKKRIGYLPENPPLYSDMRVREYLTFVARIK
                     GVNPKTINAEVDRVAERLSVLEVMDNLISNLSKGYKQRVGLAQALLNNPPVLIFDEPT
                     NGLDPRQIIEVREIIKSLGSDHTVILSTHILPEVSMTCNRVAIINEGKLVKIDTPENL
                     TKQLRGSESIILQVEGPMNEVELALSQHQSVTAVAVQPTGKENIATYRVETSIGSDIR
                     KELASLVVNRGWGLLELKAESLSLEDIFLKLTTKEEEVN"
     gene            complement(2466..3422)
                     /locus_tag="PKX09_03995"
     CDS             complement(2466..3422)
                     /locus_tag="PKX09_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17015.1"
                     /translation="MQLRRSKFNFLALFSFIFILFQLTSAQNSGIIIQTTIDTTIATI
                     GDHLHLRINLRYPIGTIFELPLVEETLGQLEVIDNQLSQPRKKGQINEQEWQLTLAVF
                     DTGQITIPALEIKAHPADDTTSVLSFQTEPKTVQVYSVLPPDKRELKDIKPPFPLRRS
                     ISWKAILLILLFLLIVGGIWYYRRWEKAHPPLIIDEKFLEPPHIVTLNQLNALKEKMP
                     TTPDEMRAFYFRISEILREFLERRFFIRALEMTTVEIAEAFKYLDIEPALTREWIELF
                     SELDIVKYAGQTPAEEDIAAVLEKACHCVESTKYETFLRRTM"
     gene            complement(3442..4320)
                     /locus_tag="PKX09_04000"
     CDS             complement(3442..4320)
                     /locus_tag="PKX09_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOU17016.1"
                     /translation="MLSKEILKQVRQIEIRTRHLVNDVFSGEYHSVFKGRGMEFSEVR
                     EYQYGDDFRSIDWNVTARYAHPYVKIQEEERELNVMLAVDASRSNAFGSQGKFKNEIA
                     AEICALLAFSAIKNNDKVGLLIFTDQVEKFIPPHKGRSHTLRVIREILYFQPQSPRTN
                     IKSGLEFLLKGLKRRAIVFLVSDFNDENFAKPLRVLNKKHDVIAIHLQDARDKILPPV
                     GLLNLKDAETGDEVWVDTSDRAFLEQYADLVEQQRQNLRNMARGMKLDLIEIDTAVSY
                     VDPLVKFFKMREQRFR"
     gene            complement(4347..5339)
                     /locus_tag="PKX09_04005"
     CDS             complement(4347..5339)
                     /locus_tag="PKX09_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOU17017.1"
                     /translation="MTFNLAEMNERIQRESAFVSKIKSEMAKVIVGQEQMIERMLIGL
                     LCNGHILLEGVPGLAKSLAVSTLAKIIQVNFRRIQFTPDLLPADLVGTMIYNQKTGTF
                     ETKRGPIFSNIILADEINRSPAKVQSALLEAMQERQVTIGETSHPLDDPFMVLATQNP
                     IEQEGTYPLPEAQVDRFMLKVIVDYPQKDEELDIVKRMARNAAIPAVNPVITAEDIRR
                     ARQTVDDIYIDEKIHKYIVDLVFATRDPKAVNLADIADLIQYGASPRASIFLAMAGKA
                     QAFIAGRGYVTPEDIRTIIWDVMRHRIILTFEAEAEEVTVEEIIRRIIETVAVP"
     gene            complement(5344..5856)
                     /locus_tag="PKX09_04010"
     CDS             complement(5344..5856)
                     /locus_tag="PKX09_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU17018.1"
                     /translation="MRRILFSLLTTGMAVLLITCSKPDNTAFGYWDNAVALFNQSDYQ
                     GCIKQYQRIIKYFPDDSLAVPAMFAMADVYKNNLNDADQAIKIYKEIIRKYGDSERAA
                     NAHFMIGYVYANEIRDLKKAEEYYKSFLKKYPTNILAPSAEWELKNLGKTLDEIPELQ
                     KITQEKQTAE"
     gene            complement(5967..8579)
                     /gene="clpB"
                     /locus_tag="PKX09_04015"
     CDS             complement(5967..8579)
                     /gene="clpB"
                     /locus_tag="PKX09_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOU17019.1"
                     /translation="MYSSEKFTIKAQEIVQNAINTAQRLSNQAIEPEHFLYAILTTPD
                     NTGQLVLNKLIGNVEPFTRSVENLLETFPKVSGGSQSYLSNASNKMLQVAEDQAKQLQ
                     DEYLSAEHLLLGIIEVAKGELKNLLKKNGISNETILSALKEIRGSQTITDQFPEDKYQ
                     ALKKYCRDLNELARRGKLDPVIGREEEIRRVLQVLARRTKNNPVLIGEPGVGKTAIAE
                     GLALRIVSQDVPESFRDKRILALDMGALIAGAKFRGEFEDRLKAVLREISEAQGKIIL
                     FIDEIHTIVGAGAAEGAVDASNLLKPALARGDLRCIAATTFSEYRKYIEKDKALERRF
                     QPVLIDEPSIEDSISILRGLKERYENHHGIRITDAALVAAVELSARYITDRFLPDKAI
                     DLIDEAASRLRLEIDSLPAELDEVERKISQLEIESRALAKEKDIEESRQRYEKIQQEL
                     AQLKDEAAVLRARWQNEKKHITAISELKKQIEAKKLEAEKAEREGNWERAAQLKHYEL
                     VELNKQLEAEKEALKNVSGKRALLREEVTEEDIAEIVSKWTHIPVSRLVEGEREKLLK
                     MEEKLHERVIGQDEAIKAVSNAVRRARAGLQDENRPLASFIFTGSTGVGKTELAKALA
                     EFLFNDENALIRIDMSEYMEKHSVSRLIGAPPGYVGYEEGGQLTEAVRRKPYSVILLD
                     EIEKAHSDVFNILLQVLEDGRLTDNKGNVANFKNSIIIMTSNLGSDLIMRQAGDVDEN
                     NIDVIYANIQETVMSQLRQILKPEFLNRIDEIIVFKPLLPKEIREVVRLQFNRLIERV
                     KTRGIQVKLSDEACDLIAKAGWDPAFGARPIKRIIQKQILDPLALEILEEKIQPGDTV
                     NIEIRDGKIHFVKG"
     gene            complement(8592..10436)
                     /locus_tag="PKX09_04020"
     CDS             complement(8592..10436)
                     /locus_tag="PKX09_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOU17020.1"
                     /translation="MKIINRNFRSYSLILTILILIWSESLFSKNEFKATEYFVNKIII
                     TGNKQFSTRKLKKQINLKEKTIWRKQTFTRRLLELDRLNLESFYIKQGYLHCTVKDSF
                     STHSNNLVDVFFKINEGQMYILKDVIISGAESLSEKKILRLLDHKINAPYNPIQIHNG
                     IKNILNEYANTGRPLTTITDSLEINNDIRLHLIIHESEPFYINQVTIVNNKLVKEKPI
                     RREIALQPGDLYSLKKIELSKRHIYETGLFSSVNIRLTDIDTVRNKVNLVVDVRELDM
                     RYLGLDFGLGQDRGLREGREPYTSLEATGQWLNRNIGGRGSRLSLKAEGALNIDTESG
                     LSQPRLSGEVTWIEPWLLGFRSSNSFTLFAETQSFDLEKNYYQTNYGGEIALIYQPER
                     RLYLKTGVAPQVIESNSQSDYTKSEEVAVTLDFRRDDRDNFLFPKNGTYLALSGKIAL
                     VGGKPADYYKLEPSFSQYFNILGPVVLAYRVKVGIMAPLRTGQPTPEYEKFYLGGSTS
                     LRAWPDNKFEPDHIDPDGYNFKGLTSVEVRFPLFWLFGGEVFVDGGNLAGEPDFSEFF
                     KTYRWNYGFGLTLATPLGPIRIDYAIKIPKENEKKQPGRFQLGIPYAF"
     gene            complement(10433..14350)
                     /locus_tag="PKX09_04025"
     CDS             complement(10433..14350)
                     /locus_tag="PKX09_04025"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOU17021.1"
                     /translation="MKKIIKISIIVIVSLVLLIGLTGFLILKTDSWKTILVSSVNRQL
                     MQNYNLKIEPEGIGGNIFYHLQFDRLKLTNQNQTELVTMSGVNLSYNLKSLIGHHHIL
                     NKLSLDTIKLAYPANIDSLLASFPEKKDTLASTGNFKLANIDVSKCLIDDSHHPGQLL
                     VSIDSLNGSLSLNADTINLQLNSTDFYVTEINEYFRIKQTAIAFVNNSLEIKNGQISN
                     RGTTAELDGRISLHKPYASQLRINVTDINPAERLPALSQVFRQDDIFDLSGEIKTSDK
                     TIKLDLGFSGTWHNQLLTNGRITGLIDGKQVNLAEMTFTKGMEQVHGTVVGNLDSSLT
                     AELRFSGIDASSWGLSAINTDLQGNLHVQTFGSIPNIDQIKVKIDLQESKIAPLDFNL
                     IRGELVYKDGLLTVLDSLYCVLGETKLIVEGEADLNGNSIDARAHFSDLNAEMLAPML
                     KMDTLSGVLDGFVEATGILTAPDLRGWIHGQQFGLPNLKFEESIARFGIVDVRTRKFG
                     DIFVEATNCQTNLIPEKIPLTSLIIHLNGDTTIVQTLKAIGENLDVEAKGYIVQFTDF
                     NLNSITILRSGNVLQNIDPLSFSLKQDTINVDAVRLRLNEGILTLTGQVVRGELQSGR
                     LEFANLSIDPINTYLKGSQGVGGKLEGMVAYKKNLEGVNYVYKINLDDAKLISQNFEN
                     IQLQGQMDNHRILIENLAVKDNQNGLLTGHGFLTCNLNSKNTKSFLSAQDSIGLTLNF
                     NRFNLGSIDDYLPSNQNIGGIVDGQINIFHYLSNPKMNCDLIIDDPVFDKLAGEKLTI
                     KGVYQNERLSITEAMLADQYGYTRGRGYLALAISIVPARFEVLKEAPLSFNFTTNSSS
                     PSFLTAYLDFLQEVQGDISLALGISGTLKNPILSGNISVKGTTIYLDPLENPITGING
                     SAVIKDNILEIVSLTGHMKKTPSSSGVGGFGDRLKSVTWDVLFPPKSDIDQPNVAING
                     TIDFTRFSAPKYNINLKGDELYIRTLLAEQEGLVSGNITLTGQDTVIYAGEIEVEDFV
                     IRNEFTGTKKAVVPKQPSKIYSIMNFHLIIPGSLEIKNSQLEGELEGDIWITRNGNEP
                     FRFSGTLDILDGQFFAYGWEFEIVRGSINLDPVEFNPTLDIEAQVDLASYGMSDTTTS
                     SSIESEVVTVYLTGYLDNPNIEFQSTNYSQSDILLFLSRAQSIGSITNGQDQLSASAA
                     NVASMWFERQLERNISRISGLDDFELRTNGNLFSSQQPDQWSVMLGRKIAPNLYIKYE
                     RSFSLIEPYQQFGLEYRINRNISIAGTYDQDGYSINYRYKYRY"
     gene            complement(14528..16339)
                     /locus_tag="PKX09_04030"
     CDS             complement(14528..16339)
                     /locus_tag="PKX09_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOU17022.1"
                     /translation="MNPLVISHRFFENVKKYADKPALLSKVDGHYRPVLYRDLGEIVK
                     AVALALDAWGIRKGDRVAILSENREEWVMSDMANQSMGAISVPIYPTLISNQIAPLFK
                     HSEPRVLFVANGTLLKKAMVTGYQFDRIVIYDPDPELPQAVAFAEMVETGRKLDAKNP
                     EAFSALLNAVEPDDIACINYTSGTTGAPKGALLTNKNFVKDVDNSVGIMKMVPEDCFL
                     SFLPLSHVLERMGGYYTPMLMGNTIGFAENIDTVVQNMAEVQPTILVSVPRLFEKIYA
                     TVMSQIESGSAITKKVFFWALKAGKNRMHSYIDKTPLTFWQKRKIKIADKLVFNKLQK
                     TTGGKIQRFVSGGAPLSKEIAEFLFSAGLPVLEGYGLTETSPVITLNGPDKVRLGSVG
                     VAIPETEFKIAEDSEILVRGPQVMKGYYKDEAATREVLDEEGFLHTGDVGYFDEDGFL
                     FITDRKKNILITAGGKNVAPQPIEDAIKLSPYISEAVLFGDRRSYIVALVTLDQEAVR
                     KWAENQHLSIDNYADFVRRPELITLIANEIERQTAHFSRFEKVKKFRIIPEDFTIESG
                     ELTPSLKVKKKAIAERYADLIDEMYQEQSQEKAQDLT"
     gene            16419..16508
                     /locus_tag="PKX09_04035"
     tRNA            16419..16508
                     /locus_tag="PKX09_04035"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16453..16455,aa:Ser,seq:gct)
     gene            complement(16658..17368)
                     /locus_tag="PKX09_04040"
     CDS             complement(16658..17368)
                     /locus_tag="PKX09_04040"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HOU17023.1"
                     /translation="MSILKTYWNYAKNPFYSIIFASPLLFAYEILIFTFNHSDIIGLR
                     NGADVLFRQFFAIFNVYGFYVVGLVVLLALMIAYHFNFRGKKKPNFEPRFFILMTLES
                     ILYALVMYFGITQIGGILMLSGSGQTTREMIVLALGAGVYEEFIFRVILITGFLFFLK
                     DILKLPEALAISLAMIGAAFIFAIFHYIGPFGEVFYLKTFILRFGAGLFLSLLYIFRG
                     YGITAYTHTIYDLIMIVL"
     gene            complement(17426..19222)
                     /gene="lepA"
                     /locus_tag="PKX09_04045"
     CDS             complement(17426..19222)
                     /gene="lepA"
                     /locus_tag="PKX09_04045"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOU17024.1"
                     /translation="MELNIRNFCIIAHIDHGKSTLADRMLEFTGTISRREMKDQVLDN
                     MDLERERGITIKSHPIQMNYHAPDGNNYILNLIDTPGHVDFSYEVSRSLAACEGALLL
                     VDASQGVEAQTVSNAYLAIENDLAIIPVINKIDLPGAMIPEAKRQIIDLIGCDESEII
                     LTSAKSGVGIEEIFEAIIKRIPPPKYEPDKPLRALIFDSVFDSFRGAIPYVRIFEGEV
                     RPGDWIRFYANNSTHEVSEVGIFKMQRQKTEKLSSGDVAYLVAGVKDISNIKVGDTIT
                     LKNNPASEPLPGYRVIKPVVFSGLYPVAAEDFEDLRSSLEKMKLNDSSLIYEPETSTA
                     LGFGFRCGYLGLLHMEIVQERLEREFNLNLITTMPNVEYQALLKTGELIEIDNPTKMP
                     AMGEIAEIHEPYVQAEIIVPTTYLGNVLKLCQDKRGIYKTTKYLDITKVQIQYELPLA
                     EIVFDFYDKLKSVSRGYASFDYEPIGYRAGDLVKVDILISGEPVDALSFIVHRDKAYA
                     HGRNLCAKLKELIPRQMFELVVQATIGSKVIARESKGPLRKNVTAKCYGGDITRKRKL
                     LEKQKEGKKRMKQIGRVEVPQEAFLAVLKMEH"
     gene            19356..20204
                     /gene="thyX"
                     /locus_tag="PKX09_04050"
     CDS             19356..20204
                     /gene="thyX"
                     /locus_tag="PKX09_04050"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOU17025.1"
                     /translation="MSHVVVPEAEALLDKEIKVLDHGFIRLVDYMGGDSRIVQSARVS
                     YGKGTKSVREDEALIDYLMRHQHTSPFEQVVLTFHCKMPIFIARQWIRHRAARVNEIS
                     GRYSVMEDEFYLPEETAIQYQNKDNRQGRDPQEVPVHLRQKVLNILAKGQQVAYDDYQ
                     KMLADDIARELARINLPLSLYTQWYWQIDLHNLFHFLELRMDEHAQWEIRQYANVIAD
                     ITRAVAPLAFRAFETHVVNAVRFSGSEIQALAANLRGQEHDLKGSRKIEYEAKLRKII
                     GNPGGE"
     gene            20370..22130
                     /locus_tag="PKX09_04055"
     CDS             20370..22130
                     /locus_tag="PKX09_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HOU17026.1"
                     /translation="MSKFQSKIFDNKIVIGVRDWVAEDVEELLQSEIFKELLNRFLDR
                     LEKKQSPLLDIFGHYPIDDNDRRILYETLCYLTKLESNLVPNIVTDSQRLFADQHLLN
                     EFVEQLYNYWRSFDRFIICDSTGDNFDKRPYRTFNLTVERLTHLVRQAYRDIQENITA
                     QHPRVYRQVHAGAEVATIAVESHSSRFKAEYSQLGSISVIRQVLIYPPLILEPPMNKR
                     KGEFIEVTENPIKSVQFNTQEWLCYPAKVGSLIVYVYFHESFYELGFALCNLFELASD
                     RDLRRKPDGIFLYGVPEENLRHLADFPTVFYEDKANDLFVGAVPRGNEFGYFGYLKKM
                     ILTLHNLRVMQKGHLPFHGAFVQILLKNGTQANVLMVGDTGAGKSETLEALRCLGDEY
                     IKDLTIIADDMGSLVIQDQVVKAYGTEIGAFLRLDDLQPGYAFGQLDRAIIMNASKTN
                     ARIVLPVTSYENIIKGVPVDYIFYANNYENVDADHPVIEKFTTPDEALYVFREGTVMS
                     KGTTTTTGLVHSYFANIFGPAQRKAIHDKIARRYFVHFFEHKVFVGQLRTRLGVAGFE
                     IEGPKIAAEELLKILLTPSN"
     gene            22439..23917
                     /gene="trpE"
                     /locus_tag="PKX09_04060"
     CDS             22439..23917
                     /gene="trpE"
                     /locus_tag="PKX09_04060"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOU17027.1"
                     /translation="MNYEDFEKLARPGLIIPVYRKFNADFITPVIAYLKLREAGKHSF
                     LLESVIKGEQLGRYSFLSQEPYLILKCRDDKTISIADHRSKIHRENFFTILQQQLKDY
                     ELLKIENLPRFTCGAVGYIGYEMAGQIEKLPKPKRDVIGSDDALLGFYNQLVAFDHLK
                     NEVILIANVFIDNDSDVRQLYLEAQNRLDCLQARLNRSYEGELRFTADLASEEANFTK
                     KDFMTAVEQAKEYILAGDIFQAVISQRFSMAYSGDVFQVYRALRNINPSPYMFFLDFL
                     DYQVIGSSPEPLIRAQDNELEIIPIAGTRQRGATSAEDDRLEAELLNDPKELAEHVML
                     VDLARNDLGRVATFGTVKVNDFKTIQKYSHVMHIISQVHGHLRDGLTVVDAFKSAFPA
                     GTVSGAPKIRAMEIINELEPEKRGIYSGAIGYFNYGGNLDLCIGIRTIVAGRKRLYYQ
                     SGAGIVADSQPENEYQETLNKCQALRQAIEQASGGINDFIHR"
     gene            23898..24494
                     /locus_tag="PKX09_04065"
     CDS             23898..24494
                     /locus_tag="PKX09_04065"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOU17028.1"
                     /translation="MILFIDNYDSFTYNLVDYIGQLHNDVLVVRNDQINVAEIENLKP
                     DGIVISPGPGRPEQACFSCQIIQTYCHKIPILGVCLGLQAIAQIFGANIVRAPFPVHG
                     KTSIIEHNGQGIFQGMTSPMTAGRYHSLIIERESLPECFTITAESSDHLIMAIAHRKF
                     PLFGVQFHPESVLTPDGMQILRNWIKIADEFKNKIEEV"
     gene            24496..25521
                     /gene="trpD"
                     /locus_tag="PKX09_04070"
     CDS             24496..25521
                     /gene="trpD"
                     /locus_tag="PKX09_04070"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOU17029.1"
                     /translation="MIKEYLEKISQCEDLTRQEAAVALQMLIDDQLTATEIGALLFGL
                     RTKGESVEEILGFVDTMQANMVAVAIDDPDAIDVCGTGGDQSHSFNVSTTAAFVVAAG
                     GVTVAKHGNRSVSSKAGSADVLEKLGARIDLSPENTRRCLNEIGISFLFAPMYHPAMK
                     AVAPHRRNLGIRTIFNMLGPLINPANLKRQLIGTYNLETAAKTAAVLRQRCYKKACVV
                     HSEEGFDEASPFAGNRVFEVDIARPDIYEYFYRAEKCSQNLNSYQLVQGGDAEKNARI
                     TLEILNGRRGYPREMTALNAAFGLYVAGKVDNIPDGIFLAEELIDSGKALQKLNQFIE
                     MSSKLAN"
     gene            25532..26329
                     /gene="trpC"
                     /locus_tag="PKX09_04075"
     CDS             25532..26329
                     /gene="trpC"
                     /locus_tag="PKX09_04075"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOU17030.1"
                     /translation="MNWLDTIVAHKKQELIDAKKAVPLEFYQAQIGDNCSKHSLLSTI
                     KKRGGFHFIGEVKRASPSRGIINSDFDPLQQAKCYTKGGASAISILTDSQFFMGNLAD
                     LQVIREQIDLPLLRKDFIVDPYQIYESRYYQANIVLLIARILSPKQITQFCELATKLA
                     LEVLLELATENDLVKIPSNHSNLILAINNRDLANFEVDLNNSFRLKPLLPDDIPVISA
                     SGISNTDECVRLFKNGFAGALIGESLMRDREPDSLLKKFRSAIANAG"
     gene            26319..26948
                     /locus_tag="PKX09_04080"
     CDS             26319..26948
                     /locus_tag="PKX09_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOU17031.1"
                     /translation="MPVKLKICGITSLEDALLAAQLGADWLGFNFYPASPRYIAPELA
                     AEIIKVLPRTVESIGVLVKPFLEEARKIITITGVQRLQIYEPQDFNDYSQLGVPVIAC
                     YRCKNGKITELNYASADMVLVDNYAESIFGGTGQAFNWRWIPTELERKKLVLAGGIDT
                     TNILEALEMIGPAVIDVASGSESKPGHKDPEKIKTLVSQIRIFNQRKDN"
     gene            26953..28128
                     /gene="trpB"
                     /locus_tag="PKX09_04085"
     CDS             26953..28128
                     /gene="trpB"
                     /locus_tag="PKX09_04085"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOU17032.1"
                     /translation="MEKGYFGEYGGQYVPEILRPALVELEEIYLALKDDPDFQKRLNI
                     ELHDYAGRPTPLYFAENLTRHFSGAKIYLKREDLNHTGAHKINNAIGQILLAQAMHKK
                     RIIAETGAGQHGVAVATVAARAGLECCIYMGAEDIQRQYPNVRRMQLLGAEVRPVHTG
                     TATLKDATNEALRDWLANVKTTHYIIGSIVGPHPFPTIVRDFQKIIGEETREQIVEKT
                     GRLPDYILACVGGGSNAMGIFYPFISDTAVNLIGVEAAGEGLASGKHSATLSCGSLGI
                     FHGMKSFVLQNNDGQIQLPYSLSAGLDYPGVGPEHCHLKDSGRVKYYAVTDTEALAAV
                     ELLCRLEGIIPALESAHALAYLEYLLPQTHSRESVVVNLSGRGDKDLETYLKYLERR"
     gene            28138..>28357
                     /locus_tag="PKX09_04090"
     CDS             28138..>28357
                     /locus_tag="PKX09_04090"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOU17033.1"
                     /translation="MSTRLNQILKSKKEKQEKCLSIFLTAGFPEKNCTPEIILTLAAS
                     GVDFIELGIPFSDPIADGPVIQKASDKAL"
     assembly_gap    28358..28457
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <28458..28751
                     /locus_tag="PKX09_04095"
     CDS             <28458..28751
                     /locus_tag="PKX09_04095"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOU17034.1"
                     /translation="TAFIYCVAYTGVTGQDNRPTPETIAFFNHLKQTLRHPWLIGFGV
                     KNHEDFQTYTHYADGVIIGSAFINLLENTPENNRLAACRTFVRNIINTPSQCT"
     gene            28896..30242
                     /locus_tag="PKX09_04100"
     CDS             28896..30242
                     /locus_tag="PKX09_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOU17035.1"
                     /translation="MKIVKSYAEINQKIKNRQAVVMTAEEIKSFIREQGIKSVLEKVD
                     VVTTGTFGAMCSSGAFLNFGHTDPPLKMEKVKINDVPAYGGLAAVDAYIGATERSQKS
                     NGHYGGGHVIEDMVAGKNLVLEAEGKPTDCYPLDSIVTEFTLKDLNQAVLLNPRNAYQ
                     RYNAAANSTDRIIYTYMGRLLPRLGNVTYSGAGELSPLNNDPNFLAIGLGTRIFLGGG
                     VGYVIDSGSQHSPETGFANLMVKGDLKLMRPEYLRGAYVYGYGCTLFVGIGVPIPLLN
                     EEIARSVAITDDELRVNIVDYGIPRLKRPILRTGVTYRELKSGQVEIEGKPVPTGSLS
                     SLFMARKIANELKNWILNGNFLLTEAIEPIPKIGKAKQLAVKPPKKSQTISTTNQKHS
                     NGIQIDHSRCILCELCIAQCPEKAIYLNENNQITIDQAKCNLCRICTKVCSVGCLE"
     gene            30256..30969
                     /locus_tag="PKX09_04105"
     CDS             30256..30969
                     /locus_tag="PKX09_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HOU17036.1"
                     /translation="MNQTVTFRYKNTIARITGEPELVKIGEEEIIRCYTDIENYISND
                     PIFKATLKPYKVPNDASKIIREMATAGAICGVGPMAAVAGAIAKYAVLAMQKAGATFA
                     IVDNGGDIAILNDRPVVVGIYSGPAVIHDIGLRFPVHSTLTSVCTSSGVIGHALSFGK
                     SHASVILADDPVLADAAATALGNRIREKDKFEIENAMNEVLKFGVAGCIVIIEDYVGF
                     AGEIPEICRVEMSESMIAK"
     gene            30986..32365
                     /locus_tag="PKX09_04110"
     CDS             30986..32365
                     /locus_tag="PKX09_04110"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOU17037.1"
                     /translation="MKVLKFGGASLKDKNAYAETIRILAEDKEPKAVVLSAIYGMTDW
                     LETTIREARANEQSIENTTTKIRAWHAKILTDLVTEPTMISDCMNELEERIQKLERLL
                     YGLAYTEEMTPQLYALIMSFGERLSVILSATIFKAHGIPAQYLESDKIGIVTDDSFTS
                     ATALLPIVRKNLPSHVYPLLEKGITPLITGFFGVNKNGKVTLFGRNSSDYSAAVIAQG
                     LKADRLEIWKDVDGFMTADPKIVATATLIDKLSYYEAAELSYFGAKILHPRTVEPLIH
                     DSIPLVIRNIYHPEIEGTEICAKEILNTAIIKSVTYNKNISVLRIKGAGVGYKPGVIS
                     EIGSRLAACGVNIYSIITSQTCINLLIDRKDSKVGYKALQVLKDGLIDSIEHCDDEVV
                     VAAVGAGLKQTPGLAAKIFSAIAGQNINIEMISLGASDVATYFIVKEPCLVPAVNALH
                     TAFFTNSIN"
     gene            32376..33446
                     /gene="asd"
                     /locus_tag="PKX09_04115"
     CDS             32376..33446
                     /gene="asd"
                     /locus_tag="PKX09_04115"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOU17038.1"
                     /translation="MKSNSQLKSKIPVGILGATGSVGQKFIELLTEHPWFEIAALAAS
                     DRSAGKTYRDAAHWFMTKPLPDNVAKMIVKNCEPNLPCRIVFSGLDASVAGEIETDFA
                     KAGYIVISNSRNHRYDPDVPILVPEVNSDHLAIIATQKYGRGAIITNPNCAATGLVVA
                     LKPLLDQFGLSAVNIVTMQAISGAGYPGVASLDIVDNVIPFISGEEAKLESEPQKILG
                     KYLDGHIEPAQFIISAACNRVAVVDGHTECVSVKLERKPTAGEIIAAWREFKGEPQRL
                     KLPSAPEKPLCYFEESSYPQPRLHRLLENGMTVSLGRLRECPILDYKFVMLSHNTVRG
                     AAGGAILNAELFIAKGGLEKLN"
     gene            33642..34502
                     /locus_tag="PKX09_04120"
     CDS             33642..34502
                     /locus_tag="PKX09_04120"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOU17039.1"
                     /translation="MRIISKNGTVNLLYQTLLLTSGSILCALAVKALILPHGLLARGM
                     TGFALIIYYIWPVIPLGLLYCLINIPVFLLGWRFVGRRFIKYSLLGMVIYSATLSLIN
                     FELNLSDPLLAVIIAGALSGAGTALILRSYGSSGGSDILCVILNKLFSITLGTGAILI
                     NAVVLILSAFIFPIEKVFYTLVFIFVSGLFTNKIFHGLANRRTAIIISDQWEIIVEKL
                     NAHRIGVTLIKGQGGYRGAGCTLLYSVIPARSVSLLKRLVTEVDAQAFIAIMEADDVT
                     GVEIGNQPHW"
     gene            34531..34950
                     /gene="rnk"
                     /locus_tag="PKX09_04125"
     CDS             34531..34950
                     /gene="rnk"
                     /locus_tag="PKX09_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HOU17040.1"
                     /translation="MTKEREIYITSQDLKRLKKELSMAGISYFHGRGDLQLLAKELDR
                     AKIIEASEIPDKIVTMNTRLIFRDLDDDSTMEVTIVFPIDANINKGKISVFSPIGTAL
                     LGYSEGDTIEWKVPAGITRIRIEKVLYQPEASGEFNL"
     gene            35206..35796
                     /locus_tag="PKX09_04130"
     CDS             35206..35796
                     /locus_tag="PKX09_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17041.1"
                     /translation="MNSLDSPLKIQTFLDETPYSTEPIYRSPLSVLRDHRAHCFDGAL
                     FAAAALRRLGYPPLIVEMLPNERDDDHLLAVYKRSNHWGAVAKSNFVGLRYREPIFRT
                     LRELILSYFEDYFNIVGEKTLRGYTLPLNLTICDRWNWMTDDEHLDEIANHLDRQRRV
                     NLLTPEMEQNLAPVDQRMLKAGLLGSNPGGLYKPDK"
     gene            complement(35925..36710)
                     /locus_tag="PKX09_04135"
     CDS             complement(35925..36710)
                     /locus_tag="PKX09_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007174341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOU17042.1"
                     /translation="MKIFILGTGRMGAWLVEEFCHQHQVAVYDRDLNKLKCFDNVERF
                     SDLNEVTSFQPELAINAVSPQNTVSAFEELLPLIPANCMLSDIASVKAGVAEFYRKIG
                     RRFVSSHPMFGPTFANIRDLRNENAIIISESDPAGKAFFRQFFENLQLNIFEYTFEEH
                     DQTTAYSLGTPFVSTLTFAANLKMQKVPGTTFRKHLEIARGLLSEDDYLLSEVLFNPY
                     TLQQIELINSRLSFLTHIIRGRDYEEMKRFLDNLRKNISALKE"
     gene            complement(36707..37762)
                     /locus_tag="PKX09_04140"
     CDS             complement(36707..37762)
                     /locus_tag="PKX09_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HOU17043.1"
                     /translation="MNLDEIRSQIDLLDGKIIKLLNDRLSQAIMAKKFKTNIEDPQRE
                     QEVFNNLELKTYDLVTPEFARRLYAIIIEEGKRLQSQDLPVIAFQGEHGAYSEMAGKA
                     WNPQLVSIPCREFAELFEGVSDGSYDYGIIPIENTLGGVVGPVNELLISTKLNVVGAI
                     EMPIRHCLLAVRNSDYRELRSAYSHPQALAQCKHFLERNKLDPIPYYDTAGAARMVAE
                     KGLKGIAAIASPLAAQLYNLEIIKEQIEDSPLNTTRFLILAHEPFTGKGEKCSIIFST
                     AHKAGTLFKVLEIFAKAKINLTRIESVPKSPGQYAFFLDFIGSKDDPKVEKVLEEVAQ
                     STSDFRLMGCYLERILA"
     gene            complement(37759..38760)
                     /locus_tag="PKX09_04145"
     CDS             complement(37759..38760)
                     /locus_tag="PKX09_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOU17044.1"
                     /translation="MNSLGRLFRLTIFGESHGPAVGVIIDGCPPGLEIKESDLTADLD
                     RRRSGAPGTTARQESDQPRFLSGVWNNHTTGAPLTIVFENTAQRSADYAEKPTVFRPG
                     HADFTAYRKFRGWNDPRGGGHFSGRMTVGLVAAGVLAKKIIAPVEVSASLIEVGGQAD
                     YSELLEQARRDGDSLGGIIECRAQNLPVGLGEPFFDSVESVLAHAIFAIPGIKGIEFG
                     TGFAAARMRGSEFNDCFIAPSGKTATNHSGGINGGITNGNELVFRIVARPTASIARSQ
                     QTLDFDSGKMTDLSISGRHDVCFALRLPVIVETLTACCLTDLLLIQKSWQLNEENNL"
     gene            complement(38753..40003)
                     /gene="aroA"
                     /locus_tag="PKX09_04150"
     CDS             complement(38753..40003)
                     /gene="aroA"
                     /locus_tag="PKX09_04150"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOU17045.1"
                     /translation="MRKSVKFSTIDGTVAAPASKSIVQRVIAMALLAEGTSRFGNVTL
                     CDDSQAAIEIARALGAEIELTERNLTLKGGFKPRKKVLDCGESGLCSRLFAAVAALSP
                     QELILTGRPALRKRPADMIPEPLQKLGARCESDNGHLPLKISGPLKGGNVKIDGSVSS
                     QFLTGLLIALPVVAADSEISVDNLKSKPYIDLTIEILEKFGIHIENYNYCRFIIPGGQ
                     KYQATEFNVEGDFSGAAFLLTAGALRGRITVTGLNPKSQQADRAILDLLQQCGARVKI
                     ASGEISVAHSKLQAFTCDANHYPDLFPPLVALAAGCEGVSQIAGVERLLYKESDRAAV
                     LKTEFGKLGIDISIEGETMLIKGGKIQGGLTKAHNDHRIAMALSIAGLCSEKPVVIAG
                     AECVTKSYPTFFDDLKSVGGNINE"
     gene            complement(39972..41276)
                     /locus_tag="PKX09_04155"
     CDS             complement(39972..41276)
                     /locus_tag="PKX09_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOU17046.1"
                     /translation="MSEKRYAIIGHPILHSKSPQIHNHWFRENNLSANYSRIIANDVY
                     VALGMGYKLGLTGANVTAPFKEKVIPSLVYLLPEAAAVGAVNTLIYNGPNISGYNTDV
                     TGVIRAFQINNVELKHKSLLVIGAGGAARAIVSALKPSGAEITIVNRPDEMALNLARS
                     FQVKSVPFSEIQTAVRTAEIIVTALPPGVRVVEPEWLNSRQIIMDANYKNSEMEPLAQ
                     AVGARFISGIVWLTEQARPAFELFTGIQPASDIPPAIIDGNPYAGKSNVILTGFMGAG
                     KSTLGPLLAERLGWQFVDVDQVIIESAGKSINAIFKDEGEEEFRRLESQALAKILESR
                     NQVIAGGGGIILSEINRALICQKALSIWLFARPLQIWPRIEQAGRPLLKSDDPLATAE
                     QIFRTRSDLYFNTADGILDTEVATPETCTDLLYAEISQIFHD"
     gene            complement(41269..41913)
                     /locus_tag="PKX09_04160"
     CDS             complement(41269..41913)
                     /locus_tag="PKX09_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOU17047.1"
                     /translation="MICVALAELNFPEIMAILKTEEMVEIRIDLLNLTETQIIAIFRS
                     HPNLVATCRKGRYSDEERQALLALAIRNGAAYVDLECDSPEDWRRSLTQLAREHHTRL
                     IISYHNFELTPGLAELHLLLRNCEALPSDIVKIVTYCQTESDAERLLSLYLLNTKPLI
                     AFGMGEAGRKSRLKALRLGTPFIYAAAETGQPTAPGQMTKNEILQLLAEESANV"
     gene            complement(41891..42928)
                     /gene="aroB"
                     /locus_tag="PKX09_04165"
     CDS             complement(41891..42928)
                     /gene="aroB"
                     /locus_tag="PKX09_04165"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOU17048.1"
                     /translation="MPAIQIKTAGKTSTIITCPSIREVFRLIEGRPVAIITDQNLDRS
                     YGDLFPEFPKIVLSNGESSKSLETVRQIYEQLGTAEVDRDYTILGIGGGVVCDIAGFV
                     ASTYLRGLDFGFVATSLLAQVDASIGGKNGVNFAGAKNIIGTINQPRFVLCPLDVLET
                     LPRAQFINGTAEIIKTGAIGDADLLEMLATQQAQFINLVPDVTEQIIFRTLNFKAGIV
                     MRDEREQGERRKLNFGHTIGHAIEIASGILHGQAVAIGMNIAAEISVRLGLLAHTEKE
                     RLEKILTAYELPTTTSVPANELLPLIFKDKKRQGEHLRFVTLKKLGSAEVINLSFNQI
                     KEFLDDLCCAG"
     gene            complement(42909..43760)
                     /gene="aroF"
                     /locus_tag="PKX09_04170"
     CDS             complement(42909..43760)
                     /gene="aroF"
                     /locus_tag="PKX09_04170"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOU17049.1"
                     /translation="MGNTENLKLTSIESHLNKTTIKVGDVTIGQDFVVIAGPCAVESE
                     EQLMQTAEAVKAAGANMLRGGAFKPRTSPYDFQGLGLKALKLLEKAKKKYGLPIVTEV
                     LDSRDVTWICEYVDILQIGARNMQNFALLKEVGKSSKPVLLKRGMYSTLKEWLNCAEY
                     ILSEGNPNVILCERGIRTFETYTRNTLDLSIVPAVREVTHLPVIVDPSHATGRLGLIK
                     PMSLAAIAAGVDGLIIEVHYKPETALSDRDQQLNPEQFKVLMRDVLSLKNFMQTLSNS
                     NNASHSN"
     gene            complement(44180..>46696)
                     /locus_tag="PKX09_04175"
     CDS             complement(44180..>46696)
                     /locus_tag="PKX09_04175"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOU17050.1"
                     /translation="GFGALFDNVEIGVMDLPYFADNYQGTLTPDAQASVTSTIRTGML
                     DLGEYFLINVVESDALNNWLGENLVHFNNVIGHLSVDPEVSELGSFYHNQSAISNFNL
                     INDGIVDIEFESSWSIQNNYNDGDELLLVTGGVDAQSNDAAKSDAKKDGATVKPSERI
                     KKVAECYSNIKILAQTAIQPAQPVKTALASKIFRPETVGSAGPLLSENFDASLEMPTG
                     WSVEDWSFGLGDVWHIANVADIKSNALYFGDPVEKKYYANSETYAYTPIIDVSAIPDS
                     EALFLEFDYACYVEEGYDYFDLYIGVLDNEDPAQIMWQMEASSEWGNFTNDGELHSIS
                     FDLSWYTRLMARNGYEKLVICFGAWSDESVSNGGILVDNVSLNTRLQDTYLSPIFGNI
                     KAGETLPVRAHINMKNLYPGDWVMVSEFWCNYYYEYYGYDYGQARQYSTFSVLNHKPV
                     AVDDTLNVLAGDTVNFMALSNDAMANDYDEDLDELFFWDATDPLYGSFKWTAIEEEGD
                     YNHDYRQYYSYVAPLNFDGLDVFEYQISDGFDSDIGKVTIRVAAEPHFVAGTQQEYTF
                     LEDSTLVLNTMRIVPGVGGVDKELYVWGKTKGTLVKIDADRVNRRLTLATKNKNAWGQ
                     ETAMLYVGHTDKPIDSLKVTVVVVPVNDPPTAGFAVTKANNTVTFTDQSNDSRDPEGA
                     IVEWLWDFGDGNTSTEQNPVHVYTAINTYTVTLSVKDNSNATAITTQNVQITNLVGIA
                     SEIAIPEKFELSQNFPNPFNPMTTIRYALPEKANVRLTIYNMLGKEVRTLVNEFEEAG
                     YKSVIWDGLDQYGRSLSTGVYIYRIQAGDFTQTRKMVFMK"
BASE COUNT        13086 a        10153 c        10218 g        13139 t
ORIGIN      
        1 gccggaatcg aatccttatc aaccaacatc agttcctcga cggcataatt ttgctgttcg
       61 atagcctgtt tggctcgttc aaagccatcg cgttcggtgc tggcgatagt gccctcgccg
      121 tgcccggtca agaaggcaac tttcttctta ccttcgcgag ttaccttaat aatggcatta
      181 ataattgctt cttcagaaaa attgggaacc agttccgctt tatcttcaca aataatcaca
      241 agattaccgt aattcttaac attatattgc ttagcaaggg ctggtttttc atccggatca
      301 acgacttccc aactaatgcg tttggaataa tggcgatatt cattgagacg atcattgatg
      361 taagacttat tggatttctt ggcaaaaacg acaatctcaa tgtctttatc caggtttttc
      421 agaatcttac gagtctgatc ggaaagacta aagatcttgg tggcggtggt atctacccgc
      481 catgaatgac gggtaaagaa aaaatcaact aatcccacaa tagcaataac tagaataata
      541 accagtacca tattgccgcc gtagattagc ctccgatttt tcagcaaagt gcggatgtcc
      601 aatttaatca aagctaacac gctggcacca aagccgagaa tcaataaaac aatcaccggc
      661 caggaccaaa tattgacgat agaaaagtaa attaacccgc aggcaattaa aattaatcca
      721 atcgaaaaaa cccatttata aattttcatg acttaacccc tccacttgga agattcaatt
      781 gactgataag taaaaaacaa cattagaaaa gtcaacgaga ggtaataaac cagatgatta
      841 gtgtcaaaga ctcctttgga gaaatcatca aaatgattga ctattgataa gtatttaaat
      901 atttcaccaa acactttacc agcaaaatct ccagcgaaac cgattaccca taagaacatt
      961 gtagctccaa aagtaccgac cgcggcaact atctggttct cggtcagcga ggaaatgaaa
     1021 ttgccgagag caacaagaac taagcccatc agcagaattc cgaggtaact aatcaaaatc
     1081 ggtacaaggc tgggattccc ccagataaat agaaaaacca taaaaagaaa agtaaccata
     1141 accattacga aataaagggt aaaagacgcc agaaattttc cgagaatcaa ctgccagtta
     1201 gtcaatggag aagttagcaa gagttccata gttccgcttt ttttctcttc cgaaaaactg
     1261 cgcatagtga tcatcggaat gacaaacaac acgatgacgc tcatattttg gaaaaacggt
     1321 cgaatcataa gaagattaac attaatcgga taaccttgtc tctggatcat taccgcatag
     1381 ctgtattcca ggaaactaga aagcaatacg tagaaaaaaa taccattgac taaaaggaac
     1441 aatccgatta caacataagc aattggagag aaaaaatatc ctttcaggtc gcgtttatat
     1501 attgccagta aattactcat ttcaattgac ctcctcttct ttggtggtga gtttcaggaa
     1561 tatatcctcg aggctcaggc tttccgcttt cagttcgagc aaaccccaac cacgattgac
     1621 caccagagat gccagctctt tacgaatatc agaaccgatt gaagtctcca ccctataagt
     1681 agctatattt tccttgcccg ttggttgaac tgccacggcg gttaccgatt gatgctgact
     1741 aagggctaat tcgacctcgt tcatcggtcc ctcgacctgc agaataatag attccgagcc
     1801 gcgcaattgc ttcgtcaagt tctcaggcgt atcaatcttg accaacttac cttcattgat
     1861 aatggcgact cgattacagg tcatactgac ttctggcaaa atatgagtcg aaaggatgac
     1921 agtatgatca ctaccgagac tcttgattat ttcacgtacc tcaattattt gacgcggatc
     1981 gagaccatta gtcggttcat cgaaaattag aaccggcgga ttattcagca gagcttgcgc
     2041 aagaccgact cgctgtttgt aacccttgga gagattgcta ataagattat ccataacttc
     2101 taaaaccgac agacgttcgg cgacacgatc gacttcggcg ttgatagttt tgggattgac
     2161 acccttgatg cgagcgacga aggtcaaata ttcacgcacg cgcatatcgg aataaagcgg
     2221 cggattctct ggtaaatagc caattcgctt tttaacctcc agaccttgtt caaagacgtc
     2281 gtacccggcc actttgaccg ttccagctga agctggcata taacccgtga taatgcgcat
     2341 cgtggtcgtt ttgccagcgg catttggtcc gaggaagccg aggatttcgc ctttttcaac
     2401 ggtgaaagaa atattatcca cggctttgac cgaaccgtaa tgttttacca aattttgtac
     2461 ttctatcaca tcgtcctccg taaaaatgtt tcatatttgg tgctttcaac acaatggcag
     2521 gctttttcca atactgccgc tatatcttct tcagccgggg tttgaccggc atatttgaca
     2581 atatcgagtt ccgagaaaag ctctatccac tccctggtca gcgcgggctc gatgtccaga
     2641 tacttaaaag cctcagcaat ttccaccgtg gtcatttcca gtgctcggat aaaaaagcgt
     2701 cgttctaaaa attcccgcag aatctccgaa atgcgaaagt aaaaggctcg catttcatcg
     2761 ggcgtggtgg gcattttctc tttcaaagca ttcagctgat tcaaagtaac tatgtgcggt
     2821 ggctccagaa atttttcatc aataattagc ggtggatggg ctttctccca gcgtctataa
     2881 taccaaattc cgccgacaat cagcaggaac agaagtatta acaaaatagc tttccatgag
     2941 atactccgac gtaaagggaa aggcggttta atgtctttca gttcacgctt atctggcggt
     3001 agaactgaat agacctgcac ggttttcggc tcagtttgaa aactcagcac gctggtagtg
     3061 tcgtccgccg ggtgagcttt gatttccagt gccggaattg tgatttgtcc agtatcgaag
     3121 acagcaaggg tcaactgcca ctcctgctcg tttatttgcc ccttcttgcg gggttgcgaa
     3181 agttggttat caattacttc taactggccg agcgtttctt cgactaacgg caactcaaaa
     3241 atcgtcccga tcggatagcg caggttgatt cgcaaatgga ggtgatcgcc gatcgttgca
     3301 atggtggtgt caatggtggt ttggataata attccggagt tctgagctga ggttaattga
     3361 aataaaataa aaataaaact aaagagggcg agaaagttaa attttgagcg acgtaactgc
     3421 attgaaaacc ttaagttcaa atcacctgaa cctttgctcc cgcatcttaa agaacttaac
     3481 cagagggtca acgtaagaaa cagcagtgtc aatttcgatt aaatccagct tcataccccg
     3541 cgccatatta cgcaaatttt gacgctgttg ctcgactaaa tcggcatact gttcgaggaa
     3601 tgcgcggtcg gaggtgtcca cccagacttc atctccggtt tcagcgtctt tcaggtttaa
     3661 taagcctacc ggcggtaata ttttatcacg agcatcctgg agatgaattg cgataacatc
     3721 gtgtttttta ttcaataccc gtaatggttt ggcgaaattt tcgtcattga aatcagaaac
     3781 cagaaagacg atcgcgcgcc gttttaagcc tttcagtaga aactccagtc ccgatttaat
     3841 attagtacgc ggcgattgtg gctgaaagta gagtatttcc cgaatcacgc gtaaagtatg
     3901 tgaccggcct ttatgaggtg gaataaattt ttcaacctga tcggtgaaga tcagtaggcc
     3961 gactttatcg ttatttttta tggcggagaa agccagtagc gcacaaattt cagccgctat
     4021 ttcattcttg aatttaccct gcgaaccgaa agcatttgag cgcgaggcat ctaccgccag
     4081 cataacgttc agttcacgct cctcttcttg aattttcaca tagggatgag cataacgggc
     4141 agtcacattc caatcaatgg aacgaaaatc atcgccatac tgatattcac gtacttccga
     4201 gaattccata ccgcgtcctt taaaaaccga atgatactca ccactaaaga catcattgac
     4261 gagatgccgg gtgcgtattt caatctgacg aacctgtttg agaatttcct tagacagcat
     4321 aacgagataa tattttgtgg aaaaaattac ggtaccgcga ccgtttcgat gatgcgcctg
     4381 ataatttctt cgactgtcac ttcttcggct tccgcctcga aagtcaggat gatgcggtgg
     4441 cgcataacat cccagataat cgtacgaatg tcttccggtg tcacataacc gcggcctgcg
     4501 ataaaagcct gcgctttacc agccatcgcc agaaatattg aagcacgtgg cgaggcacca
     4561 tactggatca aatcggcgat gtcggccagg ttgacagcct ttgggtcgcg ggtagcaaaa
     4621 accagatcga cgatatattt atgaattttt tcatcaatgt aaatatcatc aactgtttgg
     4681 cgagcacgtc gaatatcctc cgcggttata accggattga ccgctggaat agcggcgttg
     4741 cgcgccatac gtttgacaat gtcgagttct tcatctttct gcggataatc aacaatcacc
     4801 ttgagcataa aacggtcaac ctgagcctcg ggcagaggat aagtcccttc ttgttcaatc
     4861 ggattttggg tcgccaaaac cataaacggg tcgtccaacg gatgactggt ttcgccgatc
     4921 gttacctgac gttcttgcat agcttccagc aaagcagact gcaccttcgc tggcgagcgg
     4981 ttgatttcat cagccaatat aatattgcta aaaatcggac cgcgtttagt ctcaaaagtt
     5041 ccggtttttt gattataaat catcgttccg accaaatcag ccggcaaaag gtccggtgtg
     5101 aactggatgc ggcgaaaatt gacctggata attttagcca atgtgctaac agctaatgac
     5161 ttggcaaggc caggcacgcc ttctaataga atgtggccat tgcagagcaa accaatcagc
     5221 atacgttcga tcatttgttc ctgcccgacg attaccttag ccatttcact ctttatttta
     5281 gaaacaaaag cactttcgcg ctgaatgcgt tcattcattt cagctaaatt gaaggtcata
     5341 gctttactcc gcagtctgtt tttcttgggt tattttttgt aattcgggaa tttcatcgag
     5401 ggttttgccg aggtttttca gttcccattc agctgagggc gccagaatat tggtgggata
     5461 cttttttagg aaggatttat aatattcttc ggctttcttc aaatctctaa tctcattggc
     5521 atagacatat ccgatcataa aatgagcatt ggcagctcgt tcggaatcac catacttacg
     5581 aattatttct ttataaattt taatcgcttg gtcggcatca ttcagattat ttttatatac
     5641 atcagccatt gcgaacatag ccggcaccgc cagactgtca tcggggaaat atttgatgat
     5701 tctttggtac tgtttgatac atccctgata atctgactgg ttaaagagag ccacagcatt
     5761 atcccaatat ccgaaagcgg tgttgtccgg tttactacag gtaataagaa gcacagccat
     5821 tcctgttgtc aataacgaaa acaatattcg gcgcataatc caaagcctcc taaataaatc
     5881 agttaatatc taatattgat gcacaagcat tttcgcacga taattcggct ttaacggaat
     5941 ggcaattcga aatgttcctc tatcgtttag cctttgacga aatggatttt gccatcacga
     6001 atttctatat tgactgtatc gccgggctgg attttttctt ccagaatttc aagggcgagc
     6061 ggatcaagaa tctgtttctg gataatgcgt ttaataggcc gtgccccgaa agccggatcc
     6121 cagccggctt tggcaattaa atcacaagcc tcgtctgata atttgacctg aattccacgc
     6181 gttttgacac gctctatcaa gcgattgaac tgcaatctta ccacttcgcg aatttcttta
     6241 ggtaaaagcg gtttaaagac gatgatctca tcaatccggt ttaaaaattc tggcttaaga
     6301 atttgtcgca gttgtgacat aacagtttcc tggatattgg cataaatgac atcaatgttg
     6361 ttctcatcaa cgtcaccagc ctgtctcata atcaggtcgg agccaaggtt ggaggtcata
     6421 atgattatgc tatttttgaa attggcgacg ttccctttat tatcagttag acgaccatct
     6481 tccaaaactt gtagcaaaat attgaaaacg tcactatgcg ccttttcgat ttcatcgagg
     6541 aggataactg aatagggttt acggcgtacg gcttccgtaa gttggccacc ttcttcataa
     6601 ccaacatagc cgggcggagc accgatgaga cgcgagacgg agtgtttttc catatattct
     6661 gacatatcaa tgcggatcaa ggcgttttca tcattgaaaa gaaattcggc tagtgcttta
     6721 gccagctcgg ttttgccaac gccggttgag cccgtaaaga tgaaagaagc cagcgggcga
     6781 ttttcatcct gcaaaccagc gcgagctcgt cggacggcat tactgacagc tttaatcgct
     6841 tcatcttgtc cgattactcg ttcgtgcagc ttttcttcca tttttaaaag tttttcacgt
     6901 tcaccctcaa ctaaacgaga gaccgggata tgagtccatt tggagacaat ttcggcgata
     6961 tcttcttcgg taacctcttc tcgcaaaagc gcccgtttac cggaaacatt tttcaaggct
     7021 tctttttcgg cttctaattg tttgtttagc tcgactagct cgtaatgttt aagctgggcg
     7081 gctctttccc agttgccttc ccgttccgct ttttcggctt cgagtttttt cgcttcaatt
     7141 tgcttcttta actcgctgat ggcagtaata tgcttttttt cattttgcca gcgagcgcgt
     7201 agaacggcgg cttcgtcttt caactgcgcc aattcctgct gaattttttc gtacctctgc
     7261 cgggattcct caatatcttt ttctttggcg agcgcccgac tttcgatttc caactggcta
     7321 atcttgcgtt cgacttcatc cagttcagcc ggcagggaat ctatttcaag acgtagtcgg
     7381 ctagcggctt catcaatcaa gtcaatggct ttatccggta gaaaacgatc ggtaatataa
     7441 cgagctgata actctaccgc cgccactaaa gccgcatcgg taattctaat accgtggtga
     7501 ttctcgtatc tttccttgag accacgcaga atcgaaatag aatcctcaat ggaaggttca
     7561 tcaattaaaa ccggctgaaa tcggcgttca agggctttat ctttttcgat atatttacgg
     7621 tattcactga aagtggtagc cgcaatacaa cgcagatcac cacgcgccag agctggtttc
     7681 aataggttgg aggcatcaac agccccttca gccgcgccgg ctcccacgat cgtatgaatt
     7741 tcatcaataa acagaatgat ctttccctga gcttcggaaa tttcacgcag gactgctttt
     7801 aagcgatcct cgaattcacc gcggaatttg gctccggcaa tcaaagctcc catatccagc
     7861 gctaaaatac gtttatctcg gaaactctcc ggcacatctt gagaaacaat gcgcaaggct
     7921 aatccttcgg cgatagccgt tttgccgacg ccgggttcgc ctataagcac cggattattt
     7981 ttcgtgcggc gcgctaaaac ctgcaaaact cgtcggattt cttcctcacg tccgataacc
     8041 ggatcgagtt tcccgcgccg ggctaattcg ttgagatccc ggcagtattt tttcaatgcc
     8101 tgatacttat cctcaggaaa ttgatcggtg atagtttgag aaccacggat ttcttttaaa
     8161 gctgacagaa tggtttcgtt cgaaatacca ttttttttca ataaattttt taactcgcct
     8221 ttagcaactt ctattatgcc taataaaaga tgttcggcgc taagatattc atcctggagt
     8281 tgcttagcct ggtcttcagc tacctgcaac attttattgg aggcattgct taaataagat
     8341 tggcttccac cactgacttt aggaaaagtt tccagaagat tttcaacact acgagtgaaa
     8401 ggttcaacgt tcccgatcaa tttgtttaga actaattgac cagtgttgtc tggagtagtc
     8461 agaattgcat acaggaaatg ctccggttca attgcttgat tggaaagtcg ctgagcagta
     8521 ttgatggcat tttggacgat ttcttgagct ttgatggtaa atttttcact tgaatacatt
     8581 ttacatcctc cttagaatgc atagggaatt cctaattgga atctccccgg ttgttttttc
     8641 tcattctctt tagggatctt gattgcataa tcaatcctta ttggccccaa aggagtagca
     8701 agagttaggc caaatccata attccaacgg tatgtcttaa aaaattcaga aaagtcaggt
     8761 tcgcctgcta agttgccacc atcaacaaaa acttctccgc caaaaagcca aaacagcgga
     8821 aagcggactt caacgctggt taagccctta aaattatagc catcaggatc aatatggtca
     8881 ggttcaaatt tattatcagg ccaagcgcgt aaactggtag agccgccaag ataaaatttt
     8941 tcgtactcag gagtaggctg tccggttctc aaaggcgcca taataccgac ttttacccga
     9001 tatgccagaa cgaccggtcc caagatattg aaatattgac tgaaagatgg ctccaattta
     9061 tagtaatcgg cgggcttgcc accaaccaga gcaattttgc cgcttaaggc taagtaggtt
     9121 ccgtttttcg ggaagagaaa attatcgcga tcgtcacgac gaaaatctaa agtaacagct
     9181 acttcctcac ttttagtata atcactttgt gagttggatt cgattacttg cggcgccact
     9241 ccggttttga gatagagccg ccgttccggt tgatagatta aggcaatttc gccaccgtaa
     9301 ttggtctgat aatagttctt ctccaaatcg aaactttggg tttcggcgaa caaagtaaaa
     9361 gagttagagg agcggaatcc taatagccac ggttcaatcc aggtaacttc tcctgaaagg
     9421 cgaggctgag aaagtcccga ttcggtatct atattaagcg ctccctcggc cttcaggctg
     9481 aggcgactac cgcgcccgcc aatattacga ttcaaccatt gtccggtggc ttctagagaa
     9541 gtgtaaggtt cgcgaccttc cctcagacca cgatcctggc caagaccaaa atccaaacca
     9601 agataacgca tatccagctc ccggacatca actacaagat taactttgtt gcggaccgtg
     9661 tcaatatcag tcaggcgaat attgacactc gaaaaaagcc cagtttcata aatatgcctt
     9721 tttgacagtt ctattttctt caaggagtaa agatcacctg gttgtaaggc tatttcccgg
     9781 cggataggtt tttctttgac taatttatta ttgacaatag ttacttggtt gatataaaaa
     9841 ggttcggact catgaataat cagatgtagt cgaatatcat tatttatttc taacgaatct
     9901 gtaatcgtcg tcaaaggccg gccggtattg gcgtattcgt tcaggatgtt ttttatgcca
     9961 ttgtggatct gaattgggtt atatggtgca ttaattttgt gatcgagtaa tctgagaatt
    10021 tttttctcgc taagggattc agctccgcta atgattacat ctttcaggat atacatctga
    10081 ccttcattga tcttaaaaaa aacgtcaacg agattattgg agtgggttga aaatgaatct
    10141 ttaaccgtgc agtgaagata accttgttta atataaaatg attcgaggtt aaggcggtca
    10201 agttccagca gtcgccgcgt aaaggtttgc ttgcgccaga tagttttttc tttgagatta
    10261 atttgttttt ttagtttgcg agttgaaaat tgtttattgc ccgttattat aattttgttg
    10321 acaaaatatt cggttgcttt aaattcattt ttagagaaaa gcgactcact ccatattaaa
    10381 atcaaaatgg ttaaaattaa actgtatgac cgaaaatttc tgtttatgat tttcaatagc
    10441 gatacttata acggtaattt atactatatc catcctgatc ataagtccca gcaatcgaga
    10501 tgttccggtt aattcgatac tccagtccga attgttgata gggttcaatt agagaaaaag
    10561 agcgttcata ttttatatac aaattgggag cgattttacg tcccaacatt acgctccatt
    10621 ggtcaggttg ctgactcgaa aagagattgc cattggtacg caattcaaaa tcatccagtc
    10681 cactaatccg gctgatatta cgttccaatt gacgctcgaa ccacatactg gcgacatttg
    10741 cggcgctagc cgaaagttga tcctgtccat tggttattga gccgatactt tgagcccgag
    10801 ataaaaagag cagaatatcg ctttgggaat aattagttga ttgaaattcg atgttaggat
    10861 tatctaaata gccggttaaa tatactgtta ccacttcact ctcgatagaa gaggatgtgg
    10921 ttgtgtctga cataccatag gaagctagat caacttgggc ttcaatgtcc agagttggat
    10981 taaattcaac cggatcgaga ttgatcgagc ctcttactat ttcaaattcc cagccataag
    11041 cgaaaaattg tccgtcgaga atatcgagcg tccctgaaaa acggaacggt tcatttccat
    11101 tccgtgtaat ccagatgtcg ccctcgagct ctccttccaa ttgtgagttt ttaatttcaa
    11161 gactgccggg aatgatcaaa tgaaaattca taattgaata tatttttgag ggttgtttgg
    11221 gcacaaccgc ttttttcgta ccagtaaatt cgttgcggat aacgaaatct tcaacttcaa
    11281 tttctcccgc gtaaataacc gtgtcctgac cggtcaaagt aatatttccg ctaaccaagc
    11341 cttcctgctc cgccagtaaa gtacggatgt atagttcatc gccttttaga ttaatattgt
    11401 acttgggcgc agaaaaccgg gtaaagtcaa ttgtaccatt aattgcgaca ttgggttggt
    11461 caatatcact tttaggtgga aatagcacat cccaggttac agatttcagg cggtctccaa
    11521 agccacctac accgcttgat gaaggagttt ttttcatatg accagtcaga gaaacaattt
    11581 caagaatatt atcttttatc acagcactgc cgttaattcc agtaatagga ttttcgagag
    11641 ggtcgagata aatagtggtg cccttaacgg aaatatttcc ggataggatg ggatttttca
    11701 gtgtccccga aatgcccagc gcgagactta tatcaccctg gacttcttgc agaaaatcaa
    11761 gataggcagt tagaaatgat ggtgatgaac tgttcgtggt gaaattaaaa ctcagcggcg
    11821 cttcctttaa aacctcaaaa cgggcaggca caatactaat tgctagcgct aaataccctc
    11881 tgccccgagt ataaccatat tgatctgcca gcatagcttc ggtgattgaa agacgctcat
    11941 tctggtaaac acctttaata gtgagcttct ccccggccag tttgtcaaaa accggatcgt
    12001 caataatcaa atcgcagttc attttagggt tactaagata atgaaagata tttatctgcc
    12061 catctactat tccacctata ttttggttcg atggtagata gtcatctatg cttcctaaat
    12121 taaaacgatt gaaatttaag gtcaaaccga ttgaatcctg ggcactcaaa aaagactttg
    12181 tatttttaga atttagatta catgtcaaaa agccgtggcc ggtaagtagt ccattttggt
    12241 tatctttaac agctagattt tcgattagaa tccgatgatt atccatctga ccttgaagtt
    12301 gtatattttc aaaattttga ctgattaact tagcatcatc taaattaatt ttatagacat
    12361 aatttacccc ttccaggttc tttttataag ccaccatgcc ttcgagctta ccaccgacgc
    12421 cctgtgaacc ttttaaatag gtattgatcg gatcaattga gagattagca aactccagtc
    12481 ggccagactg taattcgcct cgtacaacct gcccggtgag tgtcaaaatc ccttcattca
    12541 aacgcaatct gaccgcatct acgttaatcg tatcctgttt taacgaaaag gaaagcggat
    12601 caatattttg gagaacattg ccactacgca agatagtaat actatttaaa ttgaaatccg
    12661 tgaattggac gatatagcct ttagcttcta catccagatt ttcaccgatg gcttttaaag
    12721 tctgaactat agtggtatca ccatttaaat gaatgattag cgaagttaaa ggtattttct
    12781 ccgggattaa attggtctga cagttggttg cttcaacaaa aatatcacca aatttcctgg
    12841 tacgaacatc tacaatgcca aaccgggcta tgctttcttc aaatttcaag ttcggaagcc
    12901 caaactgctg accgtgaatc caacctcgta agtccggtgc agtcaaaatt ccagtagctt
    12961 cgacaaaacc atcgagaact ccgctcaatg tatccatttt tagcatagga gctaacattt
    13021 cagcatttaa atctgaaaaa tgggcacgag catcaatact attaccattc aaatcagctt
    13081 cgccttcgac tatcaattta gtttcaccaa gcacacagta taaggaatct aaaacggtca
    13141 ataatccatc tttatagact aactctcccc gaatcagatt gaaatccaag ggtgcgattt
    13201 tactttcttg taaatctatt tttactttga tctggtctat attcggaata gaaccaaaag
    13261 tctgtacgtg taaattgcct tgcaaatcag tatttatcgc cgatagcccc cacgagctgg
    13321 cgtctattcc gctaaaacgc aattcggcgg ttaagcttga atcgagatta ccaacaacgg
    13381 tgccgtgaac ctgctccatc cctttggtaa aagtcatttc cgccaaattg acctgtttgc
    13441 catcaatcaa accagtaatt cggccgttcg tcaataattg attatgccaa gttccagaaa
    13501 atcccaaatc cagtttgatt gttttatcac tggttttaat ctctccgctc aggtcgaaaa
    13561 tatcatcttg gcggaatacc tgactgagag ccggtaatcg ttcagccgga ttaatgtcag
    13621 taacattgat tcttaactgg ctggcgtaag gtttatgcaa cgatattcga ccgtccaatt
    13681 ccgcagtcgt tccgcgattg ctgatttgac catttttaat ttccagagaa ttgttgacaa
    13741 acgcaattgc ggtctgctta atcctaaaat actcgtttat ttctgtcaca taaaaatcag
    13801 tcgaattgag ctggagatta atagtatcag cattcaggga cagcgagccg ttaagggaat
    13861 caatggatac taataattgc ccgggatggt gactgtcgtc aatcaaacat ttactcacgt
    13921 cgatatttgc cagcttaaaa ttaccggttg acgctagtgt atcctttttc tctgggaagc
    13981 tggctaataa agaatcaatg tttgcaggat aagccaactt gatagtgtca agactcaatt
    14041 tattcaatat atgatgatga ccgattaacg attttaaatt atatgacaaa ttgacacccg
    14101 acatcgtaac cagctcggtt tgattttgat tggtcagttt taatcggtca aattgtagat
    14161 gatagaaaat attgccaccg attccctcgg gctcaatttt cagattatag ttctgcatta
    14221 attgccgatt gacagaactg actaaaattg ttttccagga atcggttttt agaattaaaa
    14281 acccagtcag gccgatcaat aagaccagcg aaactattac tattattgat attttgatta
    14341 ttttcttcaa aaaaacctca acaccgggca aatttatcca cgcgttctta attctccaac
    14401 atcgtcagaa ccgtacacaa gactatttaa tcagtcacat tgccggttgt tccattaaaa
    14461 gaataggatt taccggcaat tgacagtaag tggaaaaatt agctgaataa agttgcctaa
    14521 ccgaaaatta tgtcaaatcc tgggcttttt cctgactttg ctcctgatac atctcatcaa
    14581 tcagatcagc atacctttca gcaatggcct ttttcttgac tttcaaggaa ggagttaatt
    14641 caccggattc gatcgtgaaa tcctcaggaa taatgcgaaa ctttttcact ttttcaaagc
    14701 gggaaaagtg agctgtttgt ctttctattt cgttagcaat cagggtgatc aattccggcc
    14761 gacgaacaaa atcagcataa ttgtcaattg aaagatgttg attttcggcc cattttcgaa
    14821 cggcttcttg atcgagggtc accaaagcga caatataact gcgccgatcg ccaaacaaaa
    14881 ccgcttcgga aatgtaggga cttaatttaa tcgcatcctc aattggctga ggcgcaacgt
    14941 tcttcccacc ggcagtgatc aagatattct ttttacgatc ggtgatgaat aaaaatccat
    15001 cctcatcgaa ataacctaca tcgccggtgt gtaaaaagcc ttcttcgtct aaaacttccc
    15061 gagtagccgc ttcgtctttg taatagccct tcattacttg tggtccgcga acgagaattt
    15121 cactatcttc cgcaatttta aattcggttt ccgggattgc gactccgacc gaacccagac
    15181 ggactttatc cggaccgttg agagttatca ccgggctggt ttcggttaaa ccgtaacctt
    15241 ccagaaccgg taaaccggct gagaataaaa actcggcgat ttccttagaa agcggtgcgc
    15301 caccggagac aaaccgctgg attttgccac cggttgtttt ctgtagtttg ttaaaaacca
    15361 gcttatcggc gattttgatt ttcctttttt gccagaaagt caacggtgtt ttgtcgatat
    15421 aggaatgcat tcggttttta ccagctttca atgcccaaaa aaagaccttt ttcgttatgg
    15481 ctgagccgga ttctatttgt gacatcaccg tcgcgtaaat cttttcaaac agacggggaa
    15541 cactgaccaa gattgtcggc tgaacttcag ccatattttg aactaccgta tcaatattct
    15601 cggcaaagcc gattgtgttg cccataagca ttggcgtgta gtatcctccc atcctttcca
    15661 atacgtggct aagcggcaaa aatgatagga agcaatcttc tggcaccatt ttcattatac
    15721 caacactatt gtcaacatcc ttgacaaaat ttttattggt taaaagtgcg cctttggggg
    15781 cgccggtagt gccggatgtg taatttatac aggcgatatc atcgggctca accgcattga
    15841 gaagagcgct aaaagcctcg ggattcttgg catccaactt acgtcctgtt tctaccattt
    15901 ccgcaaaagc caccgcttgc ggtagctcgg gatcgggatc ataaatcaca atacgatcaa
    15961 attgataacc agtgaccata gcttttttca gcaaagttcc attagccacg aacaaaactc
    16021 gcggttcgga atgcttgaat agcggtgcta tctgattact aatcaaggtt gggtagattg
    16081 gtacggaaat agctcccatg gattgattag ccatatcgga cataacccat tcttcgcggt
    16141 tctcggacaa tatcgccacg cgatcgccct tgcgaatacc ccacgcatcg agagctaacg
    16201 ccaccgcttt gacaatttct cctaaatccc gatataaaac cggtcggtaa tgaccatcaa
    16261 ctttggataa aagcgccggc ttatccgcat acttctttac attctcaaaa aacctgtgcg
    16321 aaattactaa aggattcata tttacctcca aaatgtaatt tgaatttaaa gaattaattc
    16381 aataaattca gccagttttt attatcgcga tttttagagg agagatggcc gagcggctga
    16441 aggcagctgc ctgctaagca gttgtactgc caaaagcggt acccagggtt cgaatccctg
    16501 tctctccgca agattttaga tgaaattgac aggatttatc ctcgtttatt tagtaaaaaa
    16561 gagcgcagag tttggcaata ttttttgttc taagaaaagc gagtagatca tttcataatt
    16621 aaaaatttta tttattcccc tgtttcttac ttccaattta taaaacgatc atgatcaaat
    16681 catatattgt atgggtataa gccgtgatgc cgtatccgcg aaatatgtaa agtaaagata
    16741 gaaacagtcc ggcgccaaaa cgcaaaatga aagtcttgag ataaaaaacc tcaccaaaag
    16801 gaccaatata atgaaatatg gcaaaaataa atgccgctcc gatcattgcc agcgaaatag
    16861 ccagcgcttc cggtagtttc agtatatcct ttaagaaaaa cagaaatccg gtgattaaaa
    16921 ttacccggaa aatgaattcc tcatagactc cggcgcccag cgccagaacg atcatctcgc
    16981 gagtcgtttg accgcttccg gacaacatta gaatgccacc gatctgagtt ataccgaaat
    17041 acataaccaa cgcatataaa atgctttcca aggtcatcag gataaaaaat cgaggttcga
    17101 aattcggctt tttcttgcct ctaaaattga agtgataggc gatcattaaa gccagtaaaa
    17161 ctactaatcc aaccacataa aagccataga cgttaaaaat cgcgaaaaat tgccgaaata
    17221 atacatccgc tccattccgc aacccgataa tatccgagtg attgaaagta aatatcaaaa
    17281 tttcgtaggc aaataataac ggcgaagcga aaatgatgct gtaaaaggga tttttagcat
    17341 agttccagta agtttttaga attgacatct taatttaata caggaaattt tggacagaaa
    17401 aatgaacgaa atccatttgg gttgattagt gttccatctt caggacggcg agaaaagctt
    17461 cttgtgggac ctcgacccgc ccgatttgct tcatgcgttt cttgccctct ttttgctttt
    17521 cgaggagttt gcgtttgcga gtaatatcgc cgccataaca tttggcggtt acatttttgc
    17581 gcaggggacc tttcgattcg cgggcaatga ctttggaacc gatggtagct tgaaccacca
    17641 attcgaacat ctggcgcggg attagttcct taagtttggc gcagagatta cgaccgtgag
    17701 cataggcttt atcgcgatga acgataaatg acaacgcatc aaccggttcg ccggaaatta
    17761 aaatgtcaac tttcactagg tcaccggctc ggtatccaat tggctcgtaa tcgaaggagg
    17821 cataaccgcg cgatactgac ttgagtttat cgtaaaaatc gaaaacgatt tcagctaagg
    17881 gcaattcata ctgaatttgc actttcgtaa tgtcgagata tttagttgtc ttgtaaatgc
    17941 cacgtttatc ctgacaaagt ttcaggacgt tccccaggta ggtggttggt acgataattt
    18001 cagcctgtac gtatggctcg tgtatttcgg caatctcgcc cattgccggc attttggttg
    18061 gattatctat ttcgataagt tcgccggttt tcagtaaagc ctgatactcg acattcggca
    18121 tcgtggttat caaattcagg ttgaattcgc gttccagacg ctcctgaacg atttccatat
    18181 gtaatagtcc taagtagcca cagcggaagc caaagccgag cgccgttgaa gtttccggtt
    18241 cgtaaatcaa gctggagtca tttagtttca tcttctccag cgatgaacgc aaatcttcaa
    18301 aatcttcagc cgctaccgga tacaagccgc taaaaactac tggcttaatg acccgatagc
    18361 cgggcagagg ttcactcgcc ggattgtttt tcagggtaat ggtgtcgccg accttaatat
    18421 tcgaaatatc tttgactcca gctaccaaat aagcgacgtc gccggaactt aatttttcag
    18481 ttttttgcct ctgcattttg aatatgccga cttcggaaac ctcgtgggtg gagttgtttg
    18541 catagaaccg aatccaatca cctggacgca cctcaccttc gaaaatccgg acataaggga
    18601 tggcgccgcg aaatgaatca aaaactgaat cgaaaatcaa ggctcttaac ggcttatccg
    18661 gttcgtattt aggtggtgga atgcgcttaa tgattgcttc gaaaatctct tcaatgccaa
    18721 caccgctctt agcgctggtc aggattatct cgctttcatc acaaccgatc aaatcaatga
    18781 tttgacgttt ggcttccgga atcattgctc ccggcaaatc tattttattt attaccggaa
    18841 taatagccag gtcattttcg atggcgagat aagcattgct gaccgtttga gcttcaactc
    18901 cctgggaggc atctaccaaa agcaaagcgc cttcacaagc cgccagactc cgcgatactt
    18961 cataggaaaa gtccacgtgt cccggagtat caatcaaatt caaaatatag ttattaccat
    19021 cgggggcgtg ataattcatc tggatcggat ggctcttgat agtaataccg cgttcgcgtt
    19081 ccaagtccat attatccagg acttgatcct tcatttcacg tcggctgata gtaccggtaa
    19141 attcgagcat ccgatcggcc agagttgatt tcccatggtc aatgtgagcg ataatgcaaa
    19201 agttacgaat attcaattcc acgtcggaga atttacggca gatacagcct gattaacaaa
    19261 aattcctata taatttttaa tgtattttga aaaattcgga tttcggtaat aataaaaatc
    19321 ctaaatttcc gaggtttaga ataaggagaa tttaaatgtc gcatgtggta gttccggaag
    19381 ccgaagcact tctggacaag gaaatcaaag ttctcgatca cggatttatt cgtctagtgg
    19441 actatatggg aggcgacagt cggattgtgc aaagcgctcg ggtttcatat ggcaagggaa
    19501 ccaaatcggt gcgagaagac gaggcgctca ttgattactt aatgcgtcac cagcatactt
    19561 cgcctttcga gcaggtggtt ttaacatttc attgcaaaat gccaattttc attgctcgac
    19621 aatggattcg ccatcgcgcg gcacgcgtca atgagatttc tggacgttat agcgttatgg
    19681 aggacgaatt ttatctccct gaggaaaccg ctattcaata tcaaaataaa gacaatcgcc
    19741 aaggacgcga tccgcaggaa gtacctgttc atctaaggca gaaagttctg aacattctag
    19801 ccaaaggcca gcaagtggct tatgacgact atcaaaaaat gttagctgac gatattgcgc
    19861 gagaattggc gcgcataaat ctgccgcttt cgttatatac tcaatggtac tggcagattg
    19921 acctccataa tcttttccac tttctggaat tgcggatgga tgaacacgcc cagtgggaaa
    19981 tacgacaata cgctaatgta atagccgata ttacgcgggc agttgcaccg ctggcattca
    20041 gggctttcga aacgcatgtt gtcaatgccg ttcgattctc cggctcggaa attcaggctt
    20101 tagccgcgaa tttgcgcggt caggaacacg acttgaaagg tagtcgcaaa atcgaatacg
    20161 aagccaaact gcgtaaaatt atcggtaacc cgggcgggga ataataatat ccattgttta
    20221 cgaaatcaag tcgtttctaa taataaaaat aatggattag caagtactga tgcaggttaa
    20281 aataattgga atacatttgc aaaataatct agtgatacta aattaaccgg ttaacttgat
    20341 tatttttatt tagaaaaggc tgtgctgtta tgagtaaatt tcaatcgaaa attttcgata
    20401 ataaaatcgt tataggcgtt cgagactggg ttgccgagga cgttgaggaa ctcctgcaga
    20461 gtgaaatttt caaagaatta ttgaatcggt tcctcgaccg cctggaaaag aagcagtcgc
    20521 ccctgctgga tattttcgga cattatccaa tagatgacaa tgatcgtaga attctttatg
    20581 aaacattatg ttatcttacc aaattggaga gtaatttagt cccgaatatc gttactgata
    20641 gccaacgatt attcgccgac caacatttgc tgaatgagtt cgtcgaacaa ttgtacaatt
    20701 actggcgcag tttcgaccgt tttatcattt gtgattcaac cggcgataat ttcgacaaac
    20761 gaccatatcg cacttttaat ttgactgtcg aacgattgac ccatttggtt cgtcaggctt
    20821 atcgtgatat tcaggaaaat attactgccc aacatccgcg agtttaccga caggtgcacg
    20881 ccggggcaga agtagccaca atcgcggtcg agtctcatag ttcgcgtttt aaagccgagt
    20941 atagccagtt aggctctatc tcggtaatcc ggcaagtctt gatttatcca ccgctgattc
    21001 tggaaccacc aatgaacaag cgcaaaggtg aatttattga agttaccgaa aacccgatca
    21061 agtcggtaca attcaatact caggaatggc tctgctatcc ggctaaagtt ggctcgctga
    21121 ttgtttatgt ctattttcac gagtcgtttt acgagttggg attcgccctt tgtaatctat
    21181 ttgagttggc gagtgatcgg gatcttcgtc ggaaaccgga tggtattttt ttgtatggag
    21241 tgccggaaga aaacctgcgc catttagccg actttccgac agttttttac gaagataaag
    21301 ccaacgattt gttcgtcggc gctgtaccgc gcgggaatga atttggttat tttggctatc
    21361 ttaaaaaaat gatcttgact ctacataact tgagggtgat gcaaaagggg catttgcctt
    21421 tccatggagc tttcgtgcaa attttgctga aaaatggaac tcaggctaat gtactgatgg
    21481 tcggcgatac cggtgctggt aaatccgaaa cattagaggc tttacgctgt ctcggtgatg
    21541 aatatattaa agacttgacc atcattgccg atgatatggg ctcgctggtt attcaagatc
    21601 aagttgtaaa agcttatggc actgaaatcg gagctttcct gcgactggat gatttgcaac
    21661 ccggctatgc ttttgggcag ctagaccggg ctatcattat gaatgccagc aaaactaacg
    21721 ctcgcattgt cctgccggtt acttcttatg aaaatatcat taagggcgta ccggttgatt
    21781 atattttcta tgctaataat tatgaaaatg ttgatgccga ccatcccgta attgaaaaat
    21841 ttaccactcc cgatgaggct ttatacgttt tccgggaagg tacggttatg agtaaaggga
    21901 caactactac caccggattg gtccatagct atttcgccaa tatttttgga ccggctcagc
    21961 gtaaagccat tcatgataaa attgcgcggc gttactttgt tcattttttt gaacataagg
    22021 ttttcgttgg gcaattgcga acgcggcttg gggttgcggg atttgagatt gagggtccga
    22081 aaattgccgc tgaggagttg ctaaaaattt tgttaacccc atccaattaa tagttttaaa
    22141 attttaagcc aaatacttaa tagattgaag ttagattgaa aattaaaata gaatcaaaaa
    22201 aacttcttga ctaccaaaga gaatttgttt aaaattgcca cgtattttta gagaattata
    22261 gatagtatgc gaatgcaaat gaacataaac tggtggtggc aaatgatgga taagcgtcat
    22321 ttcctcaggt ttaaatatga gtaaataaga aataaaaata tataaaaatt atgaaacccg
    22381 cctgagaaat gaccggcggg tttttcattt tataaaggaa aagcaaaacg agagaaatat
    22441 gaattatgag gattttgaaa aattagccag gccgggtttg attattcccg tttataggaa
    22501 attcaatgcc gattttatta cgccggtgat tgcttacctg aaattacgcg aagctgggaa
    22561 acattccttt ttactggaaa gtgtcattaa aggcgaacag ttggggcgtt attcattcct
    22621 gagtcaggag ccttatttaa ttttgaaatg ccgcgatgac aagacgattt caatcgccga
    22681 ccatagaagt aaaattcacc gagaaaattt cttcactatt ctgcaacagc agttaaaaga
    22741 ttatgaactt ctgaagattg aaaacttgcc gcgttttacc tgtggagcgg tcggatatat
    22801 cggttacgaa atggcaggtc agatcgaaaa attgccgaag cccaagcgcg atgtaatcgg
    22861 aagcgacgac gctctgctgg gcttttacaa tcagctggtg gctttcgatc atttgaaaaa
    22921 tgaagtcatt ctgatcgcta atgtttttat tgacaatgac agtgatgttc gacagttgta
    22981 tctcgaagcc caaaaccgtc tggattgtct gcaagcaaga ttaaatcgca gctatgaagg
    23041 tgaactgaga tttacggctg atctggcgag cgaggaagcc aattttacca aaaaggattt
    23101 tatgactgcc gttgagcagg ctaaggaata tattctggca ggcgatattt ttcaagccgt
    23161 gatttctcag cgtttttcga tggcttattc cggcgatgtt tttcaagtct atcgggcatt
    23221 gcgcaacatt aatccatcgc catatatgtt ttttctggac tttttggatt atcaggtaat
    23281 cggttcctca ccggaacctt tgatccgggc tcaggataat gagctggaaa tcattccaat
    23341 cgccggaact cgtcagcgag gggcaacttc cgctgaagat gatcgcctgg aggctgaact
    23401 actgaatgat cccaaagagc tggctgaaca tgttatgctg gtggatttag cccgtaatga
    23461 cctcggccgg gtagcgacat tcggcactgt taaagtcaat gattttaaaa ctattcaaaa
    23521 atattcgcac gttatgcaca tcatttctca agtgcacggt catttgcgcg atggtttgac
    23581 tgtggtagat gcctttaagt cggcttttcc agccggaacg gtttccggcg cgcctaaaat
    23641 tcgggcaatg gaaattatta atgaacttga accggaaaag cgcggaattt actccggagc
    23701 tatcggttat ttcaattatg gtggtaatct ggatttgtgt atcggtatcc gcaccatcgt
    23761 cgccggtcgg aaacgactat attaccagag tggagcggga atcgtcgcgg atagccagcc
    23821 cgaaaatgaa taccaagaaa ctctcaataa atgccaggct ctccgacagg caatagaaca
    23881 agctagtgga ggaatcaatg attttattca tagataacta tgattctttc acttataacc
    23941 ttgtggatta cattggtcaa ctgcacaacg atgtgctagt cgtgagaaat gaccaaataa
    24001 atgttgcgga gattgaaaac ttaaaaccgg atgggattgt catttcacca ggacccggtc
    24061 ggccggaaca agcctgtttt tcctgtcaga taattcaaac ttattgccat aaaattccta
    24121 ttctgggagt atgcctcgga ttacaggcga tcgctcagat attcggggct aatatcgttc
    24181 gggcaccctt tccagtgcat ggcaagactt cgattatcga acataacggt cagggaattt
    24241 ttcaaggaat gacttcaccc atgacggccg gtcgctatca ctcactgata attgagcgtg
    24301 aatctttgcc ggaatgcttt acgatcacag ccgaaagtag tgatcatctc ataatggcaa
    24361 ttgctcaccg caaatttccc cttttcggag tccaatttca tcccgaatcg gtattgaccc
    24421 cggatggaat gcaaatactg cgcaactgga taaaaattgc tgacgaattt aaaaataaga
    24481 tagaagaggt gtaatgtgat taaggaatat ctcgagaaaa taagtcaatg tgaagacctt
    24541 actcgccagg aagccgccgt ggctttgcaa atgttgattg atgatcagtt gactgccacc
    24601 gaaatcggtg ctctattatt tggattgcgc acgaaaggcg aatcggtaga ggaaattctg
    24661 ggattcgttg acacgatgca agccaatatg gtggcggtcg caattgatga tccagatgcc
    24721 attgatgttt gtggcacagg aggtgatcag agtcattcat ttaatgtttc aacgaccgcg
    24781 gcttttgtgg tggcggctgg tggagttacc gttgccaagc atggtaatcg ctcggtctcc
    24841 agcaaagccg gctccgcgga tgttctggaa aagttgggcg cccggattga tctttctccg
    24901 gaaaatactc gcagatgtct gaatgaaatc gggatcagtt ttttatttgc gccgatgtat
    24961 catccggcaa tgaaagccgt agctccgcac cgtcgaaact taggaattcg cacgattttc
    25021 aatatgcttg gaccattgat aaacccagcc aatttgaagc ggcaattgat tgggacatac
    25081 aatctggaaa ccgccgccaa aacagcggct gttttgagac aacgatgtta taaaaaagca
    25141 tgtgtggtgc atagcgagga aggctttgac gaagcatcgc cgttcgccgg taatcgggta
    25201 tttgaagttg acattgctcg accggatatt tacgaatatt tttaccgagc ggaaaaatgt
    25261 tcccaaaacc tgaacagcta tcagctggtg caaggcgggg atgccgagaa aaacgcgcgg
    25321 ataactttgg aaatattaaa tggaaggcga ggctatccgc gtgaaatgac ggctttgaat
    25381 gcggcttttg gtctttatgt cgccggtaaa gtcgacaata ttcctgatgg aatattcctt
    25441 gccgaagaac ttatcgacag cggcaaagcc ttgcagaaac tgaatcaatt tatcgagatg
    25501 agtagcaaac tcgctaatta aacgaagaga tatgaattgg cttgatacta tcgtcgctca
    25561 taaaaagcag gaattaatag acgctaagaa agccgtcccg ctggaatttt atcaagctca
    25621 aattggagac aattgctcca aacatagctt gcttagcacg ataaaaaagc ggggaggttt
    25681 tcatttcatc ggcgaagtta aaagggcttc gccgtctcga ggaattatca attctgattt
    25741 cgatccgctc cagcaggcga aatgctatac gaaaggggga gcaagtgcta tttccattct
    25801 gacggactcg caattcttta tgggtaattt ggctgatttg caagttattc gagaacagat
    25861 tgacttgccg cttctgcgta aggattttat cgttgaccct tatcagattt atgaatctcg
    25921 ttattatcaa gccaatattg tcttgctgat tgctcggatt ctatcgccaa agcaaattac
    25981 ccaattttgc gagttagcaa cgaaactggc tctggaagtt ttattagaat tggcaaccga
    26041 gaacgattta gtaaaaattc cgtctaatca ttccaattta attctggcga tcaacaaccg
    26101 tgatctggca aattttgaag tggatttgaa taattccttc cgtctgaagc cccttttacc
    26161 tgatgatata ccggtcatca gtgccagcgg aataagtaac accgacgagt gtgtacgact
    26221 ttttaaaaac ggctttgccg gtgcattaat tggcgaatca ttaatgcgtg atcgcgaacc
    26281 ggatagttta ttgaagaagt tcaggagtgc aatcgctaat gccggttaaa ttgaaaatct
    26341 gcgggattac ctcgctggag gatgctttac ttgccgctca acttggcgcc gattggctgg
    26401 gatttaattt ttatcccgca agcccgcgct acattgcacc ggaactcgcc gccgaaatta
    26461 ttaaggtgct tccgaggacg gttgagagta tcggtgtatt ggtaaaacct tttctggaag
    26521 aagctcgcaa aattatcaca atcaccggag ttcagcgctt gcagatttac gagccgcagg
    26581 atttcaatga ctattcccaa ttaggagtgc cggtaatagc ttgttaccgt tgtaaaaatg
    26641 gtaaaataac cgaattaaat tatgcgtcgg ctgatatggt tttggtggat aattatgcgg
    26701 aaagtatttt tgggggaacc ggccaagcct ttaattggcg ttggattccg accgaactgg
    26761 aacgaaagaa actggtgctg gcaggcggga tagataccac taacatcttg gaagctttag
    26821 aaatgattgg tccagccgta attgacgtag ccagcggaag tgaatcaaag cccggtcaca
    26881 aagatccgga aaagatcaag acattagtta gtcaaattag aatatttaat cagagaaagg
    26941 acaattaatt caatggaaaa aggatatttt ggcgaatatg gcggacagta tgttccggaa
    27001 atcctgcgtc cggcattggt cgaattggaa gaaatatatt tagcgcttaa agatgatccc
    27061 gattttcaaa aacggttaaa cattgagttg cacgattacg ccggtcgtcc aacgccttta
    27121 tattttgccg aaaacctgac ccgccatttt agcggggcta aaatctattt gaaacgtgag
    27181 gatttgaatc ataccggcgc tcataaaatc aacaatgcca ttgggcagat tcttctggcg
    27241 caagcaatgc ataagaagcg catcatcgcc gagactggcg ccggtcagca tggcgttgcc
    27301 gttgcgactg tggcggcgcg agccggtctg gaatgctgta tttacatggg agcggaagat
    27361 attcaacgcc aatatcccaa tgtgcgacgt atgcaacttc tgggggccga agtccgtccg
    27421 gttcataccg ggacagccac tttgaaagat gctacgaacg aagccttgcg ggattggctg
    27481 gctaatgtaa aaactactca ttatataatc ggttcgatag tcggaccaca tccgtttccc
    27541 acgattgtgc gtgactttca gaaaattatt ggcgaggaaa cacgcgaaca aattgtcgag
    27601 aaaaccggtc gtttgccgga ctacattctg gcttgtgttg gcggcgggag taacgccatg
    27661 ggtatttttt atccgtttat ttctgatacc gctgtcaatt taatcggagt agaagccgct
    27721 ggagaaggac ttgccagtgg caaacattcg gcgacactga gttgtggctc actcggtatt
    27781 tttcatggaa tgaaatcctt cgtacttcaa aataatgacg gccagattca attgccctat
    27841 tcgctctcag ccggattgga ctatccgggc gtcggtccag agcattgtca tcttaaggat
    27901 agtgggcgag tcaagtatta tgctgtaact gatactgaag ctttagccgc ggtagagctt
    27961 ttatgccgac tggaaggcat cataccggcg cttgaatccg cgcatgctct cgcttatctt
    28021 gaatatcttt tgccacagac tcatagtcgg gaatccgtag tggtgaacct gagcggtcga
    28081 ggcgataaag atctggaaac atatctcaaa tatttggaaa ggcggtaagg aaatccaatg
    28141 agcaccagat taaatcaaat tctaaaaagt aaaaaagaaa aacaggaaaa atgtctctcg
    28201 atttttctca cagccggttt tccggagaaa aattgtactc ctgagataat tttaaccctg
    28261 gctgcaagcg gggtggactt tatcgagttg ggaattccgt tttccgatcc tatcgccgac
    28321 ggtccagtta ttcagaaagc ttccgataaa gccctggnnn nnnnnnnnnn nnnnnnnnnn
    28381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28441 nnnnnnnnnn nnnnnnnacc gcttttattt actgtgtggc ttacaccggc gttaccggcc
    28501 aggataaccg accaactccc gagacaatcg catttttcaa tcatttaaaa cagactctcc
    28561 ggcatccctg gctgatcggt tttggcgtta aaaatcacga ggattttcaa acttacactc
    28621 actatgccga tggagtgatt atcggctcag ccttcattaa tttgctggaa aatacacccg
    28681 aaaataatcg ccttgccgct tgtcgcacgt ttgtccgaaa tataataaac acaccatcac
    28741 aatgtacttg atgaccttag cagaaaatta tgctgtatat ttatacataa attatacaaa
    28801 ataataagaa aatattcata aatatttaaa gtagtccgcc aataatctgg gtaaaattta
    28861 ttatattatt acgctttttt ggctgaaagg attatatgaa aattgtaaaa agctacgccg
    28921 agattaacca gaagataaaa aaccgtcagg cagttgtgat gacggcggag gagataaaga
    28981 gctttatccg ggaacagggt attaaaagtg ttttggagaa agttgatgtc gtcacgactg
    29041 gtacttttgg ggcaatgtgt tcttccggag catttttgaa ttttggtcat actgacccgc
    29101 ccttaaaaat ggaaaaagtg aaaataaacg acgttccagc atacggtggt ttagcggcgg
    29161 tggatgctta tatcggtgct accgagcgtt cccaaaaatc gaatggtcat tacggcggag
    29221 gccatgtcat agaggatatg gtagccggca aaaatctcgt cttagaagcc gaaggcaaac
    29281 ctactgattg ttatccgctt gattccattg taactgaatt tacgctcaaa gaccttaatc
    29341 aggctgttct gctaaatcca cgtaatgctt atcaacgtta taatgccgcg gctaattcga
    29401 ctgatcgtat aatttacacc tatatgggaa gattactgcc acggctcggt aatgtcacct
    29461 actccggcgc gggtgaactt tcgcctctta ataatgaccc gaattttttg gcgatcggtc
    29521 tgggaacacg tattttccta ggcggcggcg taggatatgt aattgattca ggcagtcagc
    29581 acagtccgga aacaggtttc gccaatttaa tggttaaggg cgatttgaaa cttatgcgtc
    29641 ccgaatatct acgcggagct tatgtctatg gctatggctg tactctgttc gttgggattg
    29701 gcgtgccgat tccacttttg aatgaagaaa ttgctcgttc ggttgccata accgatgacg
    29761 aactcagggt aaatattgtg gattacggaa ttccacgatt gaaacgtcca atcctgagaa
    29821 ccggagttac ataccgggag ctcaaatccg gtcaagtaga aattgagggc aagccggttc
    29881 ctacgggttc gctttccagt ctgtttatgg ctcgcaaaat tgctaatgaa ttgaaaaatt
    29941 ggattctgaa cggcaatttt ctcctgaccg aagcgatcga gccaattccc aagatcggaa
    30001 aagccaaaca attagccgtc aagccaccca aaaaatcaca gacaatatca acaaccaacc
    30061 agaaacattc caacggaatt caaattgacc attctcgttg catcctttgt gaactgtgta
    30121 tcgcccagtg tccggagaag gctatttatc taaatgaaaa caatcaaata actattgacc
    30181 aagctaaatg caatctgtgt cgtatctgca ctaaagtttg ttcagttggc tgtctggaat
    30241 aaagtaaggc aagagatgaa ccaaacggta actttccgat ataaaaatac aattgcccgc
    30301 attacgggcg agcctgaatt ggttaaaatt ggtgaggaag aaattatccg ttgttataca
    30361 gacatagaaa actatatctc caacgatcca atttttaaag cgacgttgaa gccctataaa
    30421 gttccgaacg acgccagtaa aattatacgg gaaatggcga ctgccggagc tatttgcgga
    30481 gtcggtccga tggcggcagt agccggggca attgctaaat atgcagttct ggcgatgcaa
    30541 aaagccggtg ccacctttgc tatcgttgat aacggcggtg atatcgcaat tctaaatgac
    30601 cggccagtag tggttgggat ctacagtgga ccggctgtaa ttcacgatat aggcttgcga
    30661 tttccggtgc attctacgct gactagcgtc tgtacttcat ccggggtgat cggacatgcg
    30721 ctaagcttcg ggaagtcgca cgccagcgtt attctcgctg acgatccggt gttagcggac
    30781 gccgccgcaa ctgctttagg aaatcgcatc cgtgaaaaag ataaatttga aattgaaaat
    30841 gcgatgaatg aggtactgaa attcggtgta gccggatgta tcgtaattat tgaagattat
    30901 gttggatttg cgggagaaat cccagaaatt tgccgcgttg aaatgagtga aagtatgatt
    30961 gccaaatgaa ggaatttgaa tcggtatgaa agtattgaaa ttcggtggtg ccagtttgaa
    31021 agataaaaac gcctatgcgg aaacaatccg tattttagcg gaggataaag aaccgaaggc
    31081 agtcgttcta tcagcaattt atggcatgac cgattggttg gaaactacaa tccgggaggc
    31141 tcgcgccaac gagcaatcca tcgaaaatac cacaactaag atcagagcct ggcatgctaa
    31201 aatattaacc gacttagtta ccgaaccgac tatgatatcg gactgtatga atgagctgga
    31261 agagcggatt cagaaactcg aacgcctgct ttatggtctg gcttataccg aagaaatgac
    31321 ccctcagctt tacgctctga ttatgagctt tggtgaacgg ctatcggtaa ttttgtcggc
    31381 gacgattttt aaggctcatg gcattccggc tcagtattta gagtctgata aaattggaat
    31441 tgtcaccgac gattccttta ccagtgctac cgcattgttg ccaatagttc gtaaaaattt
    31501 gccatcgcat gtctatccat tgcttgaaaa aggcattacg ccgctgatta ccggattctt
    31561 tggggttaat aaaaacggca aagttaccct ctttggccga aatagttccg actatagcgc
    31621 ggcggtcatt gcccaaggtt tgaaagccga ccgtttagaa atctggaaag atgtggatgg
    31681 atttatgacc gccgatccaa agattgtggc gacggcgacc ttaattgata aattatccta
    31741 ctacgaagcg gctgagcttt cctacttcgg tgctaaaata ttacatccgc gcacagtcga
    31801 gccgttgatt cacgactcga ttccattagt gattcgtaat atttatcatc cggagattga
    31861 aggcaccgaa atttgtgcta aagaaattct caatacggca attattaaaa gtgttactta
    31921 taataaaaac atttcggttt tacggataaa aggcgctggt gttggttata aaccgggcgt
    31981 tatttccgaa atcggcagtc gcttagccgc ctgtggagtt aatatttact cgattattac
    32041 ctcgcagacc tgtattaatt tgctgattga ccgcaaagat tctaaagtcg gctataaagc
    32101 tctccaagtt ttgaaagacg gattgattga ttcgatcgag cattgcgatg atgaagtcgt
    32161 cgtagcggca gttggcgcgg gattgaaaca gacacccggt ttagccgcga aaattttcag
    32221 tgctattgct ggccaaaata ttaacatcga aatgatttca ttgggcgctt cggatgtagc
    32281 gacctatttt atcgtcaaag agccttgttt agtgccagca gtcaatgctt tgcatacagc
    32341 tttttttacc aattctatta attaaaggga aaaaattgaa atcgaacagt cagttaaagt
    32401 ccaaaatccc agttggtatt ctgggtgcca ccggtagcgt cggacaaaaa ttcatcgaac
    32461 tattgacaga acacccctgg tttgaaattg ccgctttagc ggcttcagat cgctcggctg
    32521 gcaaaactta tcgcgatgcc gctcattggt ttatgactaa gcctttacca gacaacgttg
    32581 cgaagatgat agtaaaaaat tgtgagccaa atctaccctg tcgcattgtt ttttccgggc
    32641 tggatgcttc cgtggcagga gaaattgaaa ctgattttgc taaggccggg tatattgtaa
    32701 tttctaattc acgcaatcat cgctatgacc ccgatgtgcc gattctggta ccggaagtca
    32761 attccgatca cttggcaatt atcgctactc agaaatatgg acgaggtgcg attatcacca
    32821 atccgaattg cgccgcgacc ggtttggttg tggctctcaa acctttactc gatcaatttg
    32881 ggctttcggc cgttaatatt gttactatgc aagcgatttc cggtgctggt tatccgggcg
    32941 tcgccagtct ggatattgtg gataatgtta tcccatttat cagcggtgaa gaagcgaaat
    33001 tagaaagtga accccaaaag atactcggga aataccttga tgggcatatc gaaccagccc
    33061 aatttatcat tagcgcggct tgcaatcgtg ttgccgtcgt tgatggtcat acggaatgcg
    33121 tttcagttaa attggaacga aaaccgactg ctggagagat catcgccgcc tggcgtgaat
    33181 tcaagggtga accgcaacgt ttgaaactgc caagcgcacc ggaaaagccg ctttgctatt
    33241 ttgaagaaag cagttatccg caaccgagat tacatcgcct tctggaaaac ggtatgaccg
    33301 ttagtctcgg tcgtctgcgg gagtgtccca tcctcgatta taagtttgtg atgttatcac
    33361 ataatacggt tcggggtgcg gctggtggag ccattctcaa tgccgaactt tttattgcca
    33421 aaggcgggtt ggaaaaactt aattaatcgg tcgaatttca agcttaataa agaccgaaaa
    33481 caaggttttt ctcatcgcca tggataattg attaataccc aatgcgaccc ggtcaaagtt
    33541 ttgctgattt gaacactgcc ggatgagctt ggtttataca ctggaattgt ttatgtttgc
    33601 caaagaagat acacttttat attagtttaa ttgtaataga tatgagaatc atttcaaaaa
    33661 acggaactgt taatttatta tatcagacct tattactcac gtctggtagt attctttgtg
    33721 ctctagcggt gaaagcttta atcctaccgc atggtctgct tgcccgtgga atgacgggtt
    33781 ttgctttaat catttactat atatggccgg tgattccgct gggattgctt tattgtctta
    33841 ttaatatccc ggtttttttg ctcggttggc gttttgtggg tcgccgtttt atcaaatact
    33901 ctctgttggg aatggtgatt tattctgcga ctctttcgtt aattaatttc gagcttaatc
    33961 tatctgatcc gcttttagcc gttattattg ccggtgcttt atccggtgcg gggactgcgc
    34021 taattttacg ttcttatggt tctagtgggg gttcagacat cctttgcgta atattgaaca
    34081 aattattttc aataaccctg ggcaccggtg ccattcttat taatgcggtg gtgttgattt
    34141 tgtcagcatt tatttttcca atagagaaag ttttttacac tcttgttttc attttcgtta
    34201 gtggtctgtt tacgaataaa atttttcacg gcttggcaaa ccgtagaacc gccataatta
    34261 tctccgatca atgggaaatt atcgtcgaaa aattaaacgc acatcgaatt ggagtgactt
    34321 tgattaaagg acagggtgga tatcgcgggg ctgggtgtac attgttatat tcggtgattc
    34381 cggcgcgttc agtttcatta ttaaaacgtc tggtaacgga agtagatgcc caggcattta
    34441 ttgccattat ggaagccgat gatgtcaccg gagtggaaat cggcaatcaa ccgcattggt
    34501 aataaagcaa aaaatagaga aaggtgcaat atgacgaagg aaagggagat ttatattact
    34561 tctcaagatt taaaacgtct aaaaaaggaa ctttctatgg ctgggatttc atatttccat
    34621 ggacggggcg atttgcaact attggcgaag gagcttgaca gagccaagat tattgaagct
    34681 agcgaaatcc cagacaaaat cgtcactatg aatacgcgtc taattttccg tgatctggat
    34741 gatgatagca caatggaagt taccatcgtc tttcccattg acgctaacat taataaaggc
    34801 aaaatttcgg ttttttcgcc gatcgggacg gcacttttag gttattctga gggagatact
    34861 attgagtgga aggttccagc tggaattacc cgaattcgaa tcgaaaaagt tttataccag
    34921 ccggaagcat cgggtgagtt taatctataa caaaaggcga atttaacttg gtgagtgcca
    34981 ggaacgtagg gagctacatc tacgactttt caggactgct gataagaaga tagtgaaaat
    35041 tgagaatgcc tattattcct ttataatccc attcatagga taaatatttt tgcaaaatcg
    35101 aactcactat tgataaacag actaaattgg aaagatcgaa aaatgattca actagaccca
    35161 ataaaacatt ttgatcgtca tttaaatacg acggaacttg ctttaatgaa tagtttagat
    35221 tcgccgctga aaatccagac ttttctcgat gaaacgccct acagcaccga gcctatttac
    35281 cgatcaccgc taagtgttct acgtgatcat cgggcacatt gctttgacgg agctttgttt
    35341 gcggcggcgg ctttgcggcg actcgggtat ccaccattga ttgtagaaat gttacccaac
    35401 gagcgcgacg acgatcattt gctggcggtt tacaaacgca gtaatcactg gggtgcagta
    35461 gctaaatcca acttcgtcgg tctgcgctac cgtgagccga ttttccgcac tctgcgggag
    35521 ctaatacttt catatttcga ggattatttc aacatcgtcg gcgagaaaac cttgcgcggc
    35581 tacaccttgc cgctgaatct tactatttgc gaccgctgga attggatgac agacgatgaa
    35641 catctggatg aaatcgctaa ccatctcgat cgtcagcgtc gcgtaaattt actcacacct
    35701 gagatggaac aaaatctcgc accagttgat cagcgcatgt taaaagccgg tctgctgggc
    35761 tcgaatccgg gaggactgta taaaccggat aaataaatta ttgaccctaa tgatagaaat
    35821 ttctatcgtt cctaattttg ggattaatcc gaactactat aagaatagct gaatggcata
    35881 acctaacgcc agcattggaa atagtatcag aaaaataatg ctggttattc ttttagtgcg
    35941 ctgatgtttt tccgcaagtt atctaaaaat cgtttcattt cctcgtaatc gcgtccccga
    36001 atgatgtggg ttagaaatga cagacgagaa ttgataagtt caatttgctg gagcgtgtag
    36061 gggttaaaga gtacctccga tagtagataa tcgtcctccg aaagtagtcc gcgggcgatt
    36121 tcaagatgtt tgcggaaggt agtaccgggc actttctgca ttttcaaatt ggcggcgaag
    36181 gtcaacgtcg aaacaaaggg tgtgcctaaa gaataagccg tggtttgatc gtgttcttcg
    36241 aaggtgtatt caaaaatatt cagttgcaag ttctcaaaaa actgacggaa aaaagcctta
    36301 cctgccggat cagattcact gataataata gcgttttcgt tgcgcagatc gcgaatattg
    36361 gcaaaagtag gtccgaacat cggatgagat gaaacgaagc gccggccgat tttacggtaa
    36421 aattcagcca caccggcttt aaccgaggca atatcagaca gcatacaatt agcaggaatg
    36481 agcggcagga gttcctcgaa agccgatacg gtattttgcg ggctaacggc attgatagcc
    36541 agctccggtt gaaagctcgt cacctcattt aaatcggaaa aacgttcaac attgtcgaaa
    36601 catttcaatt tatttaaatc gcgatcgtaa actgcaacct ggtgttgatg acaaaattct
    36661 tccaccagcc aggcacccat acggcccgtt cccagaataa atattttcac gccagaatcc
    36721 tttctaaata gcatcccatc aagcgaaaat cagaggtaga ttgtgcgact tcttctaaaa
    36781 ctttttcgac tttgggatca tctttagagc cgataaaatc gaggaaaaag gcatattgac
    36841 cgggcgattt ggggactgat tcgatgcggg tcaggttgat tttcgccttg gcaaaaattt
    36901 cgagcacttt gaagagcgta ccggctttat gagcggtcga gaaaatgatt gaacattttt
    36961 cgccttttcc ggtgaacggt tcatgagcca gaatcaaaaa gcgagtagtg ttcaaaggtg
    37021 aatcttcgat ctgctctttg ataatttcca gattgtaaag ttgagcggct agcggactgg
    37081 cgattgcggc aattcctttc aaaccttttt cagcaaccat acgagccgcg cccgccgtgt
    37141 cgtagtaggg tatcgggtcg agtttattac gttctaaaaa atgtttacac tgggctaaag
    37201 cttgcggatg ggaataggca ctgcgcagtt cgcggtaatc gctattgcgc accgccagta
    37261 aacaatgacg tatcggcatt tctattgcac ccacgacatt caatttagtg gaaatgagca
    37321 attcattgac tggtccaact actccgccga gagtattttc aatgggaata atcccgtaat
    37381 cgtaggagcc gtcgctaacg ccttcgaata gttcggcaaa ttcgcggcaa ggaatgctga
    37441 ctaattgcgg attccaggct ttgccagcca tttcgctata ggcgccgtgt tcgccctgga
    37501 aagcgattac cggtaggtct tgagattgca agcgtttgcc ttcttcgatg ataatggcgt
    37561 ataaacgtcg agcgaactcc ggagtgacca agtcgtaagt ttttagttcc agattattga
    37621 aaacctcttg ctcgcgctga gggtcttcga tattggtttt gaattttttg gccataattg
    37681 cctgtgacag acggtcattg agcaatttga ttattttgcc gtcgagcagg tcaatctgtg
    37741 accgaatttc atccaggttc ataagttatt ttcctcattt aattgccatg atttttgaat
    37801 taataacaag tcggttagac agcaagccgt cagagtctca actatcaccg gcaggcgtaa
    37861 agcaaagcag acatcgtgcc taccgctgat ggataaatct gtcatttttc cgctatcaaa
    37921 atctaaagtt tgctgactgc gggcaatgct ggcagtcggt cgagctacga tgcggaaaac
    37981 gagctcgttg ccattggtaa ttccaccatt tattccgcca gaatgattcg tagcggtctt
    38041 gccgctcggt gcgatgaagc agtcattgaa ttcactaccg cgcatacgag cggcagcgaa
    38101 tccagtgccg aactcgatgc ctttgatacc gggaatggca aagatggcat gcgccagaac
    38161 cgattctacc gaatcgaaaa agggttcgcc tagcccgacc ggcaaatttt gagcgcggca
    38221 ttcgatgatc ccgcccagcg agtcgccgtc gcggcgggct tgctcgagca gttccgaata
    38281 atcagcctga ccgccaactt ctatgagaga agcggaaacc tcgaccggtg caataatttt
    38341 cttagccagc acgccggcgg cgaccaagcc gaccgtcatc cgtccggaaa agtgtccccc
    38401 gccacgcgga tcattccagc ctcgaaattt gcgataagcg gtaaagtcgg cgtgtcccgg
    38461 ccggaagacg gtcggctttt cggcgtaatc cgccgatcgc tgggcggtgt tctcaaagac
    38521 aatcgtcagc ggcgcgccgg ttgtgtgatt attccaaaca ccactcaaaa atcgtggctg
    38581 atcactttcc tgacgagctg ttgtacccgg agcaccactc cgacggcgat cgagatcagc
    38641 agttaaatca ctttctttaa tttccaatcc cggagggcaa ccgtcaatta ttaccccgac
    38701 cgccggtccg tgtgactcgc cgaaaatcgt taggcggaag agtcgtccca aactattcat
    38761 tgatattacc tccaactgat tttaaatcgt cgaaaaatgt cgggtaggac ttggtaacac
    38821 attcggcgcc cgcgatcaca accggctttt ccgaacatag cccggcaatc gacagcgcca
    38881 tagcaatgcg gtgatcgtta tgggcttttg tcaaaccacc ttgaatttta ccacctttga
    38941 ttaacatcgt ttcgccttct atactaatat caattccaag cttcccgaac tccgtcttca
    39001 aaaccgccgc tcgatcactt tctttgtaaa gtaagcgttc aacgcccgcg atttggctga
    39061 caccttcaca ccctgccgcc agagccacca acggtggaaa aagatccgga taatggtttg
    39121 catcgcaagt aaatgcctgc aatttagaat gagcgacgct aatctcaccc gaggcgattt
    39181 ttacgcgagc accgcattgt tgtagtaaat ccaaaattgc tcgatcagcc tgctgggact
    39241 tgggattaag tcctgtcacg gtaatccgcc cccgcaaagc gccagcggtc aataaaaaag
    39301 ccgcaccact gaagtcacct tcgacattga attcggtcgc ttgatatttc tgaccgcccg
    39361 gaataatgaa acggcagtaa ttataatttt cgatgtggat accaaatttt tccaaaattt
    39421 caatggttaa gtcaatatat ggtttgcttt ttagattatc aacgctgatt tcagaatcgg
    39481 cggcgacaac cggcaaagcg atcagtaaac cggttaaaaa ctgcgaactt acggaaccgt
    39541 caatctttac attgccccct tttagcggac cgctgatctt taatggtaaa tgaccattgt
    39601 cggattcgca tcgggctccg agtttctgaa gcggttcggg aatcatatct gccggacgtt
    39661 tgcgaagtgc cggacgacca gtgagaatca actcctgtgg gctgagcgcc gctaccgccg
    39721 caaacaagcg tgaacacaat cccgattcgc cgcaatccag tactttttta cgtggcttga
    39781 aaccaccttt tagggtaaga ttacgctcag tcaattctat ttctgcaccg agcgctcggg
    39841 cgatttcgat agccgcctga ctgtcatcgc aaagcgtaac attgccgaag cgggaagtac
    39901 cttccgccag caaagccatc gcaataacgc gctgaacgat gctcttagaa gccggagcgg
    39961 cgacggtacc gtcaatcgtg gaaaatttga ctgatttccg catagagtaa atccgtacag
    40021 gtttccggcg tggcaacttc agtatctaaa ataccgtcag ccgtgttgaa atataaatcg
    40081 gagcgcgtcc tgaaaatttg ttcagcagta gctaatgggt catcgctttt tagtagtgga
    40141 cgaccggctt gttcaattcg tggccagatt tggagcggtc gggcaaagag ccagatactc
    40201 agtgcttttt ggcaaatcaa cgcccgattt atctcgctta aaatgattcc tccacctccg
    40261 gcgatgactt gatttcggga ttctaaaatt tttgccagcg cttgcgactc taaccggcga
    40321 aattcttctt caccttcatc cttaaaaatg gcgttgatac ttttaccggc tgattcgata
    40381 ataacttgat caacatcaac aaattgccag cccagtcgct cggctaacag cggcccgagt
    40441 gtcgatttac cggcgcccat aaatccggtt aagatgacat tggactttcc ggcatacgga
    40501 ttgccatcta tgatcgcagg tggaatgtcc gaagcgggtt gaataccagt gaataattcg
    40561 aacgctggac gggcttgctc ggttagccag actattccgg aaataaaccg agcgccaact
    40621 gcttgcgcca gcggctccat ttcagaattt ttataattgg cgtccattat tatctgacgg
    40681 gaattgagcc attctggttc aaccacgcgc actcccggcg gaagcgccgt cacgattatt
    40741 tcggccgttc gaacagcagt ttgaatttca ctgaagggca cagatttaac ttgaaaactg
    40801 cgcgccagat tcaaagccat ttcatccgga cggttcacaa tggtaatctc ggcgccagaa
    40861 ggtttgaggg cgctgacgat cgcccgcgcg gcgccgcccg caccgattac aaggagagac
    40921 ttgtgcttta attcaacatt attaatttga aaagcacgaa taaccccggt aacatcagta
    40981 ttgtagccgc taatattagg accattatag atcagagtat tgacggctcc aaccgcggcg
    41041 gcttctggca agaggtaaac taatgatggt atgacttttt ccttgaacgg cgcggtgacg
    41101 ttggcgccgg taagaccgag cttgtatccc attccgagcg caacatatac gtcattggcg
    41161 attatccggc tataattggc ggacagatta ttttcacgaa accagtgatt atgaatctgg
    41221 gggcttttgc tatgcagaat tggatgcccg ataatggcat agcgtttttc agacattcgc
    41281 cgactcctca gcaagcagtt gcagaatttc atttttcgtc atttgtccgg gcgcagtagg
    41341 ttgaccggtt tcagccgcgg cgtaaatgaa aggcgtaccg agtcggagcg ctttaaggcg
    41401 cgatttccga ccggcttcgc ccatcccaaa agcaatcagc ggtttggtgt ttagcaaata
    41461 taaacttaac aaacgttccg catcgctttc cgtttgacaa tatgttacaa tcttgacaat
    41521 atcagaaggt aatgcctcac aatttcgcaa aagcagatgt aattccgcca aacccggcgt
    41581 cagttcgaaa ttatgatacg agataataag gcgggtatga tgttcgcgcg ctaattgggt
    41641 cagcgatctg cgccagtctt ctggagaatc gcattcgaga tcaacgtaag ccgccccgtt
    41701 ccgaatggca agcgccagta aagcctgacg ctcttcgtcg gaatagcggc ctttgcggca
    41761 agttgcaacc agattcgggt ggcttcggaa aattgcgatt atctgggttt cagtcaaatt
    41821 gagcaaatca atccggattt ctaccatttc ttctgttttt aaaatagcca ttatttctgg
    41881 gaaattgagt tcagccagcg caacacagat catcgagaaa ttccttaatt tgattaaatg
    41941 aaagattaat tacttcagct gagcccagct ttttcaatgt tacaaaacgc agatgctcgc
    42001 cctgacgttt tttatctttg aaaattaacg gcaggagctc attggccggt actgaggtag
    42061 tggtcggcaa ttcgtaagcg gttaatattt tctcaagacg ttctttttca gtatgcgcca
    42121 ataaacccaa tcgcaccgag atttccgcgg ctatattcat tccgatcgct accgcctggc
    42181 cgtgcagaat gcctgaggca atttcaatag cgtgcccgat cgtgtgacca aagttaagtt
    42241 tgcgacgctc gccttgttcc cgttcgtcac gcatcacgat gccagctttg aaattcagcg
    42301 tgcggaaaat gatttgctca gtcacatcag gcacgaggtt gatgaattgg gcttgttggg
    42361 ttgccaacat ttccaataaa tcggcgtcgc cgatggcacc ggttttgata atttcagccg
    42421 tgccattgat aaactgcgcg cgcggtaagg tctccagcac atccagcgga caaagcacaa
    42481 aacgtggctg attgatcgtg ccgataatgt tttttgcacc ggcaaaatta acgccgtttt
    42541 taccgccgat gctggcatca acttgagcca gcagagaagt cgctacaaat ccgaaatcta
    42601 atccgcgcag gtaagtgctg gctacgaacc cggcgatgtc acaaaccacg ccaccaccga
    42661 ttcccagaat tgtataatcg cggtccactt cggcagtgcc taactgttca taaatctgcc
    42721 ggacagtctc aagactcttg gagctctcgc cgttcgacaa aacgattttg ggaaattcag
    42781 gaaacagatc gccgtaggaa cgatcgagat tttggtcggt tattatagcg actggtcgcc
    42841 cttcgatcaa tcggaagact tcccgaattg aaggacaagt tataatcgtg gaagttttgc
    42901 cagcagtttt aatttgaatg gctggcattg ttcgaattac ttagagtttg catgaaattt
    42961 ttcagactca atacatcgcg cataagcact ttgaattgtt ccggattcag ttgctggtcg
    43021 cgatcgctaa gagcggtttc cggtttataa tgaacttcga tgattaatcc gtccacaccg
    43081 gcagctatcg cggctaaaga catcggcttg attagtccta atcgtcccgt ggcatgcgac
    43141 ggatcaacga taactggtag gtgagtcact tctcggacag ccggaacaat gctcaggtcg
    43201 agcgtatttc gggtgtaagt ttcaaaggtg cgaataccgc gttcgcacaa aatcacattc
    43261 ggattacctt cggaaagaat gtactcagcg cagtttagcc attctttcaa ggtcgaatac
    43321 ataccgcgtt tcagcaacac cggcttggaa ctcttaccca cttctttcag aagcgcaaaa
    43381 ttctgcatat tgcgagcacc aatctgaaga atgtcaacat attcgcagat ccaggtgaca
    43441 tcgcgcgaat ccaacacttc ggtgacaatc ggcaatccat atttcttttt agctttttct
    43501 aataatttta aagcctttaa accgagccct tggaaatcgt agggcgaagt gcgcggtttg
    43561 aaagcgccac cgcgcaacat attagctccg gcggctttaa ccgcctcagc agtttgcatt
    43621 aattgctctt ctgattcgac ggcgcaaggt ccggcgataa cgacgaaatc ctgtccgatg
    43681 gttacatcac caaccttgat agtagtctta tttaagtgac tttcgatgga agtcagtttt
    43741 agattttcgg tattacccat ttatttctcc ttttcaagtc tatctgtaac aaaaacccct
    43801 tcgcgctgga aggggtcttt aatatattga tttaaaagta aggttacccc ttccggtgca
    43861 attccttaaa ataaaaatag taaaaaaagt atcttttgtt attcatatca gtctttcacc
    43921 aaaaataacg ttatatgtta acatataaaa acttattatt caataatttt tttatggaat
    43981 aaaaatgcct ttgattatct ccctgggtaa ttcactggag aattcggggg ttattctcat
    44041 cacggaacct gttgcccgta atccgtaact ggtaacttct tctctctctt ttatttgtct
    44101 tagtattagc ctgttcgaag tataaaaaag aagccctccg aaatggaggg cttctagtta
    44161 ttaatttaaa aaaggtaggt tatttcataa agaccatttt tctggtctgg gtgaaatctc
    44221 cagcctggat cctgtagata tagacgccgg tacttaatga tcgtccatat tgatcaagac
    44281 catcccagat aaccgactta tacccagctt cttcaaactc attgaccaaa gtacgaactt
    44341 ccttgccaag catattgtag atcgttaacc gtacattggc tttctccggt aaagcataac
    44401 gaattgtcgt catcgggttg aatgggttcg ggaagttctg ggacaattcg aacttttccg
    44461 gaatagcaat ttcgctggca ataccaacga gattagtgat ctgaacattt tgagttgtaa
    44521 ttgccgtggc attgctattg tccttaactg acaaggttac cgtataagtg ttgattgctg
    44581 tatagacgtg aactggattc tgttcagtgc tggtattgcc atcaccgaaa tcccagagcc
    44641 attcgacaat agcaccttcc ggatcgcgag aatcattcga ttggtcggtg aatgtaaccg
    44701 tattgttggc tttggtaacc gcgaaaccgg ccgtcggcgg gtcattcacc ggcacgacta
    44761 cgacggtaac cttaagcgaa tcaatgggct tatccgtatg accgacataa agcatagctg
    44821 tttcctgtcc ccaagcattc ttgttcttgg tcgcgagagt caggcgacga ttgacacggt
    44881 cagcatcgat cttgactaat gtacccttgg ttttgcccca gacgtataat tccttgtcaa
    44941 ctccaccgac accgggaact atgcgcattg tattgagtac tagcgtggaa tcttccagaa
    45001 aagtatattc ttgttgagtg ccagcgacaa aatgtggctc ggcggctacg cggatggtta
    45061 ctttaccaat gtctgaatca aaaccatcac tgatttgata ctcaaagacg tcaagtccat
    45121 caaagttcag aggagcaaca taagagtagt actgacgata gtcatgatta taatcaccct
    45181 cttcctcgat agcagtccat ttgaatgagc cgtacagagg atcagtcgcg tcccagaaga
    45241 ataactcatc caggtcctca tcgtagtcat tagccatagc atcgttactg agtgccataa
    45301 aattgacagt atcaccagct aggacattca aagtatcatc caccgcgact ggcttgtggt
    45361 tcagtacact gaaggtgcta tattgccgcg cctgaccata atcataccca taatattcgt
    45421 aataataatt acaccagaat tccgatacca taacccagtc accagggtag agattcttca
    45481 tattgatgtg agctctgaca ggcagagttt caccggcttt gatattacca aagattgggc
    45541 tgaggtaagt atcctgcagt ctggtattga gtgaaacatt atcaaccagg atgccgccat
    45601 tactgacaga ttcatcagac caagccccaa aacaaatcac caatttttca tagccgtttc
    45661 gggccattaa tctagtatac caactcaaat caaatgagat ggagtgtaat tcgccgtcat
    45721 tcgtaaaatt accccattca cttgaagctt ccatttgcca cataatctgg gctggatctt
    45781 cgttgtcgag aaccccaata tagaggtcga aataatcgta gccttcttcg acatagcagg
    45841 cgtaatcgaa ctccaggaag agtgcttcgg aatccggaat agcgcttacg tcaataattg
    45901 gcgtataggc gtaggtttcc gaattggcat aatatttctt ttcaaccgga tcaccaaagt
    45961 aaagggcatt acttttgata tcggcaacat tcgcgatatg ccatacatca cccaggccga
    46021 atgaccaatc ttcgacactc cagccggtgg gcatttccag actggcgtcg aaattctccg
    46081 acagcagagg accagccgaa ccaaccgttt ccggtcggaa gattttagaa gccagagccg
    46141 ttttaaccgg ttgagcgggt tgaattgcag tttgcgctaa tattttgata ttagaataac
    46201 actcggcgac tttcttgatt ctttcagaag gtttcaccgt agcgccatcc ttcttggcat
    46261 ccgattttgc ggcatcgttt gattgagcat ctactccacc agtaactaat aaaagttcat
    46321 caccatcatt gtaattattt tggatggacc agctactctc aaattcaata tccacaatac
    46381 catcgttgat taagttaaaa ttggagatag cggactggtt atgatagaaa ctacccagct
    46441 cacttacttc cggatcaacc gagagatgac cgataacatt attgaaatga accaggttct
    46501 caccaagcca attattcaag gcatcagatt cgactacgtt aatcagaaaa tattcgccta
    46561 aatccagcat accagttcgg attgtgctgg tgacagacgc ctgtgcatct ggagtaagcg
    46621 tgccttggta attatcggcg aaatatggca aatccattac tccgatttca acattatcga
    46681 atagagcacc gaaacc
//
LOCUS       DAOSMP010000015        41213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_1009_length_41213_cov_21.788498, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000015 DAOSMP010000000
VERSION     DAOSMP010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 41213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41213
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1009_length_41213_cov_21.788498"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PKX09_04180"
     CDS             <1..486
                     /locus_tag="PKX09_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17051.1"
                     /translation="SIPQLHTVDYPTWLKIASIGQFRYIPKPLSTWRRHSQQLTRQRI
                     VPLTKAMIPFLLKTQDSLPPQIARNVNISTSQLQHYWEKMMTIILIRGGRYKLIVRDW
                     ETARQYYWESFRKWPAFIPFWRVKAILGILMSYFHGNMEWTTSIRVAFSKIKSKIVRK
                     A"
     gene            486..1997
                     /locus_tag="PKX09_04185"
     CDS             486..1997
                     /locus_tag="PKX09_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOU17052.1"
                     /translation="MIDLIKTSIKDSFIYSFGNIMLRISGFILLPVYLTQLSPAEYGI
                     LGLLEVTTQVLVQIFSLAMHLPLIRWYWAPEYHNKQKTIFFSTIMFSSLLAGLMLIIF
                     LPWASTFAQFLFHDTNYANLIQLMLVSASLQIITMIPTTLARVQGKAILYTISHILNF
                     IVSLLLTLFFILKLDMKVEGIYLAQILGYIVNILFLGRFIWQNSELNFDFTILKEMLN
                     YSFPLALSSMASVLLTIADRYTLNILGQLSDVGVYSLGFKISNTLNVLVISSINLALT
                     PLIYQKMEDPNNKRFYSKIMTYMTFGIMFFVLGLSLYSQEIVQLFAKDKAYWPAYRLV
                     PILCFAIVFGMLKDLSTIGLNLKKKTKIIGLIVTIISFINVGLNFLFVSLLKTTGAAL
                     AMLLTQVIFFFVMFQAAQKQYRIPYEIQKIFKMLLLGGFLIGIGFFLFNENEPLIVRI
                     FSKLFLIILFPIGLYLWNFYEPIEIQTIKGAWRKWRNPLNWKKNRSKIKLDRK"
     gene            2011..4551
                     /locus_tag="PKX09_04190"
     CDS             2011..4551
                     /locus_tag="PKX09_04190"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOU17053.1"
                     /translation="MSKKTKKSTSAKNVPPQNKKPVNWLATINPRKATIVAFLIVLLI
                     LIIFYKPYVIDRLEPAGGDRMASIAQSKFINDYREQTGEIPLWNPAIFCGVPIYYALW
                     SNSFNLDYLISKISKVIDWKLSWFILATVGMFLIFWLLEFPWYYALLGTIAFLFYPHY
                     QALITVGHFAKVRAVCAMPLAVFGFLYLVKKRNFLSFLLFVIFFSLQLRTQHYQIVFY
                     TLLVFMALGIRQIVEWVKTKQAQKIYISLGLFVAGLVTSVLMSAQPLFVVNEYTPYST
                     RGGQAINLKEDAAQAEVKSSGVTFEYATRWSLNPKELATLIVPRFYGGTSQEPYTGKA
                     YPQLRGQPIPGYWGDMPFTQSCEYMGILIVILALLGLWYYRKDGVVISLFILLVFSLL
                     LGFGSHFPILYKPLFKYLPYFSKFRVPSMILILVDFILVILSLYGLKGLVEKFDLAKY
                     KSALMVSGFFILFGLFFLLAPGVLSYTSAQDAQYMNNPQVIEMLRTARREFLQADTLR
                     MLVFVICFGALVVLYKMKKIRPDFLIGAIIILVAVDLINVSKRFLRADELVDTKSLER
                     RYFAETSFDKILKQDQEIFRILGLGNHLQSNDLAYRYQIVTGYSPIKPQLIQDLIDNN
                     LVAGPTQSSLNWNVINMLNAKYIIAPGMLNEPNLTILDVNQQRKEVLYLNEGVLPRAY
                     FVSEVRFLPSEKDVVAFMNTTEFDPAKMALTSVALDTSAGFDTGGSVQVTDYTPNRVV
                     LNVETERPAFLVLADAYYPKGWTARVNGVETPIYQVNHVLRGVSVPAGNYALEFKFLP
                     RSYQIASQISTISCDIVWLSLLGVLIYQNREKIKNLKKKRPTPAKSNR"
     gene            4594..>5458
                     /locus_tag="PKX09_04195"
     CDS             4594..>5458
                     /locus_tag="PKX09_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17054.1"
                     /translation="MKINIYRHSGNIKRLLLVLAIVMIFALLNYTQKIVTRLRQDSAN
                     LIRFYADIYAKAAMDMSGDDFSFIFEEIIQKTSTPMILSDAEAMEPTAWRNIGVPDND
                     RSPATLAKVKKIMQEMDRENQPIPLKYEDRVLQYIHFGDTNMIRQLRRLPYIEIAIVG
                     LFILLGYVGFSLIRENEKRSIWVGMAKETAHQLGTPLTALMGWLELLKDEIGDNANIS
                     EIARDIQRLEKVANRFSQIGSRPVLKPTNINSVINDAVSYYRRRLPQLGPGISLEFIP
                     SGDYIVNLNADL"
     assembly_gap    5459..5558
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5559..5954
                     /locus_tag="PKX09_04200"
     CDS             <5559..5954
                     /locus_tag="PKX09_04200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU17055.1"
                     /translation="SLEFIPSGDYIVNLNADLFSWALENLIKNAIDAVSQENGKIRVE
                     LHLLHGGKKIAVDVIDNGKGISKNNRKNIFRPGYSTKQRGWGLGLSLAMRIVKEYHRG
                     QLFVLMSKPNEKTVMRIVLKNPQENLNRG"
     gene            6336..7697
                     /locus_tag="PKX09_04205"
     CDS             6336..7697
                     /locus_tag="PKX09_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOU17056.1"
                     /translation="MKQHKFTLSESEMPKAWYNIAADLPYALPPALHPGTQKPITPDD
                     LAPIFPMSLIEQEVSTERFIPIPESVLEIYQLWRPTPLVRAYRLEKAIGTHSKIYFKN
                     ESVSPPGSHKPNTAVAQAYYNYKEGVKRLTTETGAGQWGSALAFACNYFGLECVVFMV
                     RISYETKPYRKSMIETWGGRIFASPSQETASGRAILANDPNCAGSLGMAISEAVEVAM
                     NDKNTKYSLGSVLNHVMLHQTIIGEETQKQIAQTGDYPDIIIGCVGGGSNFAGFSFPF
                     IRDKIAGKKLRAIAVEPTACPSLTKGLYSYDFGDTAKLTPLLAMYTLGHNFMPSKIHA
                     GGLRYHGASPLVSSMVKEGLIEARSYQQTEVFKAAVTFARSEGIIPAPETAHAIKATI
                     DEAKKADEEGVTKTIMFNFSGHGHFDMSAYDSYFAGRLEDNHFSDASLQKNLAELADL
                     PKI"
     gene            7811..8575
                     /locus_tag="PKX09_04210"
     CDS             7811..8575
                     /locus_tag="PKX09_04210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOU17057.1"
                     /translation="MKVLYSKPQVKTIQIDGLGSVTLRRDHRARRLTLKVKPDQQITL
                     TVPYSISEKTALAFLEANREWALKNIQALKEIKPPIFIIEDKQTIQTRTHRLALQPTS
                     GDEIRVGIQPGIINVQYPSDQKVSHPRIQEAIRWGLTAAYRIEAKQFLPPRVRDLAQR
                     YGFRYNRLFIKNLKSRWGSCSAANNINLNLHLMRLPDELIDYVILHELTHTQIKNHSP
                     LFWQELERMLPDAKVRRQQLKTITVQGFLPSVVMEN"
     gene            8680..10605
                     /locus_tag="PKX09_04215"
     CDS             8680..10605
                     /locus_tag="PKX09_04215"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010884405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOU17058.1"
                     /translation="MYKSQKTSLKSEDQDSKSGANPFPDCLDTADWRKLTALQNQYVE
                     AIVTEYIRLCRPAKVSILSDTPEEIAYVRQLALRTGEEQPLKIAGHTVHFDGYNDQGR
                     DKEHTRILVNDGHLNSERLNTLDRVKGLKEIHQLLDGIMAGKEMLVRFYCLGPLGSIF
                     SIPALQITDSSYVAHSEDLLYRQGYSEFRRLNGSDRFFHFIHSAGELDERGCSKNVDK
                     RRIYIDLESNRVFTINNQYAGNSVGLKKLALRLAINKANHEDWLAEHMFIMGVHPLQK
                     KRTTYFTGAFPSACGKTSTAMLPGQTIVGDDIAYLRVSNDKKCNAVNIESGIFGIIAD
                     VNPIDDALIYRCLTTPRELIFSNVLVKDGKPYWLGMGCETPESGINHSGEWFKGKKDA
                     AGKEIPLAHKNARYTMRLSELENLDPRADDPNGVPISAIIYGGRDSDTNPPILQALDW
                     LHGVMLGATLESETTAATLGQEGQRTFSPMANLDFVVVPLGLYIENHLKFGARLAKAP
                     LVFSTNYFLKDSSGNYLNEKTDKKVWILWAEGRVHNEYDAIATPVGYLPQYNDLQNLF
                     KLALGKDYSRTAYEQQFTLRIDKFIAKLDRVSEFYRNETVPAEFNNRLAQQRADLVKL
                     QEEYQAPEISPFQIVKR"
     gene            10715..10927
                     /gene="rpmE"
                     /locus_tag="PKX09_04220"
     CDS             10715..10927
                     /gene="rpmE"
                     /locus_tag="PKX09_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOU17059.1"
                     /translation="MKPNIHPEYKECTVTCACGNTFVTRSTVGDLKVEICSACHPFFT
                     GKQKLIDSAGRIEKFNRKYKRDTQKG"
     gene            10988..11941
                     /locus_tag="PKX09_04225"
     CDS             10988..11941
                     /locus_tag="PKX09_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOU17060.1"
                     /translation="MAQKILVGGQAVIEGVMMRVPGAYATAVRRPDGEIEIKRENFTS
                     IADRIPFFKKPLLRGIISMFESLKIGLATIQLSADIAMQAENAAKPADKTKDRIATTI
                     SMILAFGLAFLLFGFLPLWLTTELLNIERKALAFNLVVGAWRILFFLMYLWLISFMKD
                     IQRLFQYHGAEHKVVFTFESGQDLTVENAQPFKTYHPRCGTSFIFIVMMVGILLFALI
                     DAVVIWGFGSISLVARLIYHLALLPLVAGIGYEFLKLSAKFQHKRFTQWLIAPGLWLQ
                     RITTKPPTDDQIEVAIAALKGAFGEHYAEFAGREYIAEAVD"
     gene            11945..13003
                     /gene="prfA"
                     /locus_tag="PKX09_04230"
     CDS             11945..13003
                     /gene="prfA"
                     /locus_tag="PKX09_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOU17061.1"
                     /translation="MREQIQTIIKRYNDITDEMSRPEVIADQHRYRELAKEHKDLTPI
                     VDTGNHYLQVLKNIDDDKAILEGHDEDLKQLAHEELATLEEEKSRLFEELKKLLIPKD
                     PNDDKNAIVEIRAGTGGEEAAIFAADLYRLYLKFAERKGWKVEELSGTQNGLGGFKEV
                     IFLLSGENVYGTMKFESGVHRVQRVPVTETQGRIHTSAATVAVLPEAEEVDIEINASE
                     LRIDTYRSSGAGGQHVNKVETAIRITHLPTGLVVTCQDEKSQYKNKEKALKILRSRLL
                     ALKEAEKEAEIAAKRRSMVSTGDRSAKIRTYNFPQGRVTDHRINLTLYRLTEVMEGEI
                     DELISALQLAHNTELMKS"
     gene            13111..13992
                     /gene="prmC"
                     /locus_tag="PKX09_04235"
     CDS             13111..13992
                     /gene="prmC"
                     /locus_tag="PKX09_04235"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOU17062.1"
                     /translation="MAERERQIWRIVDVLKWSRDYLKGKQIESPQIEAEWILREVLNF
                     NRLEIYLKYERPLSPAELARIKALLLKRASGQPIQYVLGSAEFMGLKLRVTPDVLIPR
                     PETELLVEKTLELCRNRVTARILDIGTGSGCIAIALAHFLPECQVVAIDVSATALQIA
                     RENAAIHNVANRIDFQELDILHSPELTMGQFDIIVSNPPYVSGDYYVNLPKLVKEHEP
                     EIALNPGTDGLLFYRRIRELASKIAIPGGAVVLEIGGDYQATAVAELFSESAFDSVAI
                     VKDYSGQSRVIIAELAK"
     gene            13989..14828
                     /locus_tag="PKX09_04240"
     CDS             13989..14828
                     /locus_tag="PKX09_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOU17063.1"
                     /translation="MKNLLAYFKIMRPLNLLQGGIAILVCMALADPKPEWLSVLLALV
                     IVWAYTGAGNILNDYFDVEIDRINRPQRPIPAGLIQPRTALFYAIFLFALGTAAVVPV
                     FNRQLAIIIPFALFFLITYTKFFKPRPLWGNLIVSAILGMTFLFGSSIFGDIRVGIVP
                     CLLAFGFNFVREIVKDMQDVAGDQARGARTFPLVYGLTAARRLVIVATLILMVGALVP
                     FYLKIYGKFYLLILVLTVEIPLIYVIFSISRDASATNCGRIAAILKGDIFFGLLAIFA
                     GKF"
     gene            14829..18260
                     /locus_tag="PKX09_04245"
     CDS             14829..18260
                     /locus_tag="PKX09_04245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOU17064.1"
                     /translation="MTQFVHLHNHSDYSLLDGAQSIESLVNRAVELSMPAVGLTEHGN
                     LFSAIHFLKAANTSGLKPILGCEIYVADNNRFDKKSREQGGSGYHHLLLLVQNRQGYE
                     NLIKLVSLGYLEGYYYRPRVDNELLRKYNAGLIATSACIKGRIPDLAIKGDYEKAKQL
                     ALEYAEIFAGRFYLEVQNHGIDDELRWYEIAKKLSAEIGLPRLLTNDTHYTLPEHWEA
                     HDAHLCIGTGKEMSDPDRMKYEQHEYWVKSAEEMAALYPNDPEIIENTLKIAEQCDFN
                     LEMNQYYLPEFPIAESAKVKTADEYLAQLIEERLPGRYPEITPEIQERVDYELGVIRQ
                     MGFAGYFLIVQDFVHYAKNNNIPVGPGRGSAAGSIVAYILGITNIDPLKYKLIFERFL
                     NPERISMPDIDIDFCDTKRGAVIDYIRKKYGENCVAQIITFGKMKARAVIRDVGRVLS
                     MPLAEVNRIAKLVPEQINITLETAINNTPELQEAVNIDEQHQKLFAISKVLEGLNRHA
                     STHAAGVVIAPGNLTDYVPLYQSSNGEVTTQYDMKCIDQIGLLKVDLLGIRNLSVIDN
                     TLAMLKAKGIELDIDKIPEKDEETLALFGEGRTIGIFQFESSGMREYLKKLKPSGIED
                     LIAMNALYRPGPMEMINDFIDRKQGRKPITYLHPLLEPILVDTYGIIVYQEQVMQIAH
                     QVAGFSLAKADLMRKAMGKKQKKTMESLKKEFVEGAIKNGIKRSVAVQIYELIQRFAE
                     YGFNRSHSAAYAILAYQCGYLKAHYPAEFMAANLSSEINDTDRITILANEARNMGYEI
                     LPPDVNYSEVRFIPEGKAIRYGLHAIKNVGEKAAENIVATRQTEKQFRTLFQMVAALD
                     LKSVNRKVLESLIAAGAMDSLEGTRGQKFAAIDTAIQYATHIQEEKNNNQVNLFGIAN
                     GAPQATLSEPRLPDVAPWEDSEKWNKEKELLGMYLSGHPLLEYSTEIESFSNYDFTEP
                     LSNLDKSNVKLGGLIASIKTMTDRKGRPMAFINLESLNGKVELIAFADIYEKYREHLK
                     INHPIFVSGNVNCRNEDDAKIIVEEISPLRGLLNRKSKKLHVRINVNQFGAEDLENLK
                     ILLAKHTGECQLFLHLYDGNGVNKSIRSGNLRVSADRELLDILKKTFGEENAWVE"
     gene            18260..18706
                     /gene="dtd"
                     /locus_tag="PKX09_04250"
     CDS             18260..18706
                     /gene="dtd"
                     /locus_tag="PKX09_04250"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOU17065.1"
                     /translation="MIAVIQRVSQAHVTVENKIVGRIGPGLVILLGVFQNDGDADAAF
                     LADKIAGLRIFGDEQQKMNLSIKDVGGAALVISQFTLCGDWRKGRRPSFISAADPETG
                     RRLYEKFIADLKIAGVPTEQGIFGAMMAVSLVNDGPVTFVLDSKLR"
     gene            18711..19424
                     /gene="radC"
                     /locus_tag="PKX09_04255"
     CDS             18711..19424
                     /gene="radC"
                     /locus_tag="PKX09_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOU17066.1"
                     /translation="MRSKEPAVYRLPITAWPESDRPREKLMKAGPMKLTEAELLAILL
                     RSGAGGNSALDVAREILRKSGGLNALAQMDYHDILNLKIRGIGPTKAVTIAAGLQLAR
                     RLQAVSVSPPEMIIHKSDEVAKIYIPRLQDLKKEIFMVVLLASNNKIIRDAIISEGIL
                     NASVVTPREVFREAILGMAAAIILIHNHPSGNPEPSNEDLQLTRQMKEVGQAMNIPVL
                     DHIIIAGAQYTSFADKELL"
     gene            19645..20910
                     /locus_tag="PKX09_04260"
     CDS             19645..20910
                     /locus_tag="PKX09_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17067.1"
                     /translation="MEIIERATLQQHITNTPDSYLRPLLAALYLEDQDCQAAIELCRQ
                     DLAIHPFSTIGHYIWALAALAMNDLNQAAIHLRAIIQIDGKFLQAYYKLAELGGDYLN
                     QAELKYCYEKICDLNPYDKKSTDKLSAFPLELQSQKPQSPAKPERTDQVEESSEKAIM
                     AEPIESAPEIDPLTGANRLSQFFESIKKPTESLKSSGIQPTPVPEEEAHFGPIEKTAI
                     ETEPTVEPEPTISFDEPFPEEPQVKESSGSSSISDLFSRLREKPFEEVQKENWLKEHL
                     SGETPSPAMPTITESDKPAVEPQAVSQPQSETKATDIPAKSKATATPKVAPTKAGKAS
                     QKPKSASNKQRQSPQPKKSAASPGNISFPIPTWTLVDILMKQKLYEQALNILDIIESK
                     SKNEKDLEKVQASRAKIVRLMAEEQSGEA"
     gene            22015..22215
                     /locus_tag="PKX09_04265"
     CDS             22015..22215
                     /locus_tag="PKX09_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17068.1"
                     /translation="MDNYESAITALKIPFFPLGLRFPAGDVLDAVMIFRTNNFSRPDC
                     FLSHVSCLLSPFLSLSLFTNDQ"
     gene            complement(22230..23072)
                     /gene="ispE"
                     /locus_tag="PKX09_04270"
     CDS             complement(22230..23072)
                     /gene="ispE"
                     /locus_tag="PKX09_04270"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOU17069.1"
                     /translation="MPKATSIKIKSNAKINLTLRITGRRPDGYHNLSTIFQEIDLHDV
                     LTFTPAKTFEFATNHLALSNKTDNLCAKAYRRMEQIAPHSQPWRITLEKNIPIGAGLG
                     GGSSNAATVLKFLNKAWQINLSDDNLARIGCAIGSDVPFYIKGKTQGARGIGEVLEPL
                     ILMEDFVILLFLPEINISTAWAYRQFNPRNAKPAYNFAELISGGRVDWVKFENQFEEI
                     VFPVYPQLAKLKEQILNTGALYAGLSGSGAALFGIFRNRTYALKAARQLPEWRTIITK
                     PVKE"
     gene            complement(23050..24549)
                     /gene="gltX"
                     /locus_tag="PKX09_04275"
     CDS             complement(23050..24549)
                     /gene="gltX"
                     /locus_tag="PKX09_04275"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOU17070.1"
                     /translation="MIRTRFAPSPTGFLHVGGLRTALYNFLFAKKYNGRFILRIEDTD
                     QRRKVAGAVENLLATLEWAGVKPDEGPQIGGDFGPYVQSERLQLYRDYAQILLEKGHA
                     YYCFCSAERLKKLKEWQIAQKIPPRYDNHCRDLSVEEIQQHLSSGEEYVIRMKIPAAG
                     AIEVDDLIRGKVTFPVSQIDDQVLLKSDGYPTYHLANVVDDHLMQISHVIRGEEWLPS
                     TPKHVLLYRYFGWELPQFAHLSLLLNPDKSKLSKRQGDVAVEDYRRKGYLPEALINFL
                     ALLGWNPGDDREIFSLDELIREFSLERVSHSGAIFNIEKLNWMNGIYIRNLSLERLWE
                     YAKPYLEECLHQPGVDERAHWDEARLKAALNSVKDGLSNFSEIPQKTAFYFASSISYE
                     APEIQDYLTTPESITVLKTLIPLIDSVGINQTDGKKPDFNSDSFIAAVRNVQKATGIK
                     GKPLWMTIRVALTGTTQGPEIHQIASIIGREICIKRLEAALNYAESHLN"
     assembly_gap    24552..24651
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(24755..25246)
                     /gene="ispF"
                     /locus_tag="PKX09_04280"
     CDS             complement(24755..25246)
                     /gene="ispF"
                     /locus_tag="PKX09_04280"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOU17071.1"
                     /translation="MRYRFGSGIDVHRFCSGRPLILGGVTIPFDKGLLGHSDADVLTH
                     AIIDALLGASALGDIGTHFPDTDAQYKDISSLILLEKTREMVVSVGFRVSQIDATVIT
                     QAPRLLPFIPEIRHSLADCLQIPVGDISVKAKTTEGLGFIGSGEGIAALATVTLVMLG
                     TSR"
     gene            complement(25248..25952)
                     /gene="ispD"
                     /locus_tag="PKX09_04285"
     CDS             complement(25248..25952)
                     /gene="ispD"
                     /locus_tag="PKX09_04285"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOU17072.1"
                     /translation="MFPNTAFISGIVVAAGQGKRFGLGIPKQFLKIKGQPLLAYTIDN
                     LLKIGILSELIVVVSYDRINSKELKAFLPLPSSVPIKIIAGGEHRQDSVFNGMQVIDS
                     QAEIVVIHDGVRPFVMPELVRETVRLCSEFDGAILAIPAVDTLKEVEDGQIVRTIERS
                     RVWQAQTPQTFRREVLERAYANARQNNIIATDDAMLVELIGGKVAVVKSTPDNLKITC
                     QNDLKIAKSILKLREP"
     gene            complement(25962..26996)
                     /gene="queA"
                     /locus_tag="PKX09_04290"
     CDS             complement(25962..26996)
                     /gene="queA"
                     /locus_tag="PKX09_04290"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOU17073.1"
                     /translation="MRLSDFDYNVPEELIAKYPVSKRDHSRLMVIDRKKQTITHKRFY
                     NIIDYLNPGDLLVINETKVYPARLWAVKDRTEAKVEVFLLRELENSLWEVMVKPARKV
                     RIGNKLTIAKGVQCDVIDNTVSGGRVVRFNNIPKETLYKLIDEVGLSPLPPYIDREPT
                     EDDKETYQTVYAKKRGAVAAPTAGLHFTEELMQKIKDKGVKIFPIVLHIGLGTFRPVT
                     VEDLSRHRMDSEYFEVSAETALAINNARSKSKRIIAVGTSVVRTLETVTVSGFQITPR
                     RGWTDKFIHPPYEFKMVDALITNFHQPKSTTIMQVSAFGGHELIMRAYQEAIEKKYRF
                     FSYGDSMLII"
     gene            complement(27008..28027)
                     /gene="ruvB"
                     /locus_tag="PKX09_04295"
     CDS             complement(27008..28027)
                     /gene="ruvB"
                     /locus_tag="PKX09_04295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOU17074.1"
                     /translation="MHSKMIDPQKLAEDYEYEKTLRPSAFEEFVGQKNVVENLKLYIS
                     AAQERGEALDHVLLFGPPGLGKTTLAYIVAKELGVNIRTSSGPVVERAGDLAGMLTNL
                     AARDVFFIDEIHRLNNVVEEYLYSAMEDYHIDIVIDKGPNARSIQLNLEQFTLIGATT
                     RLGHLTSPLRDRFGVILRMDYYNPDELFQILMRTAQILNIELRPDGAAEIAKRSRGTP
                     RVANRILRRTRDYAQVKAAGVIDKKVAEQSLKMLEIDELGLDNLDRQILLTIIDKFSG
                     GPVGLNNLAVAVGEEAETIEDVYEPYLIQQGLIQRTARGRQATPATYKYFHKNISSSQ
                     QKLFT"
     gene            complement(28264..29514)
                     /locus_tag="PKX09_04300"
     CDS             complement(28264..29514)
                     /locus_tag="PKX09_04300"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOU17075.1"
                     /translation="MPLTAVIGAQWGDEGKGKIVDILSESADLVARFQGGANAGHTVQ
                     IGTEETILHQIPSGILRSQTHCLLGNGMAFDPFGLKNEIDVVQRKGIDVRNRLHISPL
                     AQVVTPLHKILDNYYENISGARAIGTTKRGIGPCYTDKISRNGIRVCDLFNENDLREN
                     ITSRTIFMRKQGVLTESEEDKLADDIEVFLDAVREILPYVDNTIFRIHSYLESGKNVL
                     AEGAQGVLLDIDFGTYPFVTASNTIAGNVATGLGIGPHWIDSIIGVFKCYTTRVGAGP
                     FPTELNDAVGDHLQARGDEFGATTRRRRRCGWFDAALGKYAVKINGFSSLAFTKLDVL
                     DDLAEIKICTHYANGEFPLLDLKNAIPVYLTLPGWQKSTTEAHSLDELPFQARQYLQT
                     LEDLLEVKISYISTGKERRQIILV"
     gene            complement(29582..30463)
                     /gene="secF"
                     /locus_tag="PKX09_04305"
     CDS             complement(29582..30463)
                     /gene="secF"
                     /locus_tag="PKX09_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOU17076.1"
                     /translation="MRRIIKKTDIPFIKYSKTAITISLVIIAIGLISLIFRGLNFGID
                     FTGGTLIQVKFEQPRTIEDIRNDLATINLENSIIQKSGENEYIIRVPQDLSSANLVDV
                     IKSTLGISDQNILRQEQVGPKIGKELQGAALLAIFFSLIVMGIYIAIRFKFQYSIGVI
                     LSLLHDVLITLTVFTIFQLEVSLSTIAAFLTIIGYSLNDTIVVFDRVRENLKLHRNET
                     YERIMDISINETLSRTIITDMTTLFVVLCLLLVPGEVRIFAIAMTIGSIAGTYSTIYI
                     AGPLVVFWSRRFKRSTK"
     gene            complement(30481..32394)
                     /gene="secD"
                     /locus_tag="PKX09_04310"
     CDS             complement(30481..32394)
                     /gene="secD"
                     /locus_tag="PKX09_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOU17077.1"
                     /translation="MFKKNIVRFIIIGVVVVLACYSLYWTVAYHSFSDEKREAMRADG
                     SLDKYERRVIRLGLDLQGGMHIVMEVDLPKLIESLASNKTPQFMDLLKEANDEAIANE
                     EDFFDVFRRKINESDLKLVRHFNGRGFKNDEILKSLEEEAKDAVDRAMEVIRNRVDQF
                     GVSEPSIQKSGKYRIIVELAGIQDIGRARELIQSTALLEFILVKDAAVTQQFITAVDN
                     YLKTGKADEVSPETHEEADSIVALKESKDKAISVDELLGTTSTETGLTAEQADTGLIV
                     DEEIYKERPFSSLLRNIDNQIGVPERNVYAVKKILEDPNVKKLIPFDSKFLWSAKPVR
                     RTLQDGRTENFYILYHVHSEPALLGKYITKASASLGGAGSQSSGQPIVNISMNSEGAR
                     IFAKVTGANIGKQLAVVLDDKVYTAPYIRVKIPDGSAYIEGMSDMEEAKDIAIVLRAG
                     ALPAPMTIVEERTVGPSLGKDSIAVGIRVGIFALLVIMLFMFFYYRRSGLLADMALIL
                     NMVFTLAVMAMLRATLTLPGIAGLVLTIGMAVDANVLIFERIREELERGKTVSAAVST
                     GYSRALSAILDSNITTLLTAIILMQTGTGPVKGFAVTLFWGIASSMFTAIFVTRTFFN
                     WRIEKHAIKTLSI"
     gene            complement(32509..33960)
                     /locus_tag="PKX09_04315"
     CDS             complement(32509..33960)
                     /locus_tag="PKX09_04315"
                     /inference="COORDINATES: protein motif:HMM:NF028343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14/M99 family metallopeptidase"
                     /protein_id="HOU17078.1"
                     /translation="MKKNKTRKTILLIGLIISWQVASGLTAEIKRPSREHEVYFENTP
                     NELHVYKLYGRFDGNTIFILGGIQGDEPGGYLSADLYPDLVLDRGNLIVIPRANFHSI
                     ILNKRGVNGDMNRRFDRKQSTDIEDQIVAIIKQLMSESDLFLNLHDGSGFYSKTYIDK
                     NRNPYCYGQSIIADADHVVLNGDTLKLEEMAQKVIKGINLRVENPEHKFSFFNTNTLS
                     PDTKYPEQRKSATYYALTTFHIPAFGIESSKNLENVELKIRYHNYAINEFFKLMGVEP
                     EHPAVLFEPPKLIYLLITINNTETKIVENDGFIQVNYGDIIRVKHIESNYNRGLTCDV
                     LGIGNAQDFQKPIPVLKPTSIIVRRDNIIIGSVKVETIGSTFDSIAYIIEVNGIQRKL
                     TDGQTLTVKRGDLIKIIEVLIPGLDSADLQVNLKGFVPPGSYNDGEDRNCLVDTRKLQ
                     WKKYSVDGLGKKYPIVVTYGSDELTRAFIYIQD"
     gene            complement(33947..35200)
                     /locus_tag="PKX09_04320"
     CDS             complement(33947..35200)
                     /locus_tag="PKX09_04320"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOU17079.1"
                     /translation="MARKISILILIAVLNAVSLAENLFSNVLNISADSTGNVLLVDKS
                     HQKMFVVRSNAPGALEIVKEYRITTGRRRGDKERSGDLKTPEGIYYIIGRVPDNRLTA
                     RYGPLAYALDYPNYVDRLQKRNGSNIWIHGRDEEIKDFITEGCISLENSHILDLRKYI
                     VVRQTPVIILDNLSDYYVAPENLKAAVSFWTEYINGWTKNWDSGAVAEYCDYYAPNFL
                     DENGRNLKQFKSYKTDLEKRYQWKTVMVDEISVLVSDCEAHLKFRQRYLCPTFYSEGQ
                     KQLILVPIDSNWRIVRETFRPTSPSLTTEEMVTQFLSGWKSAWEQKDIDTYINFYGEE
                     FTSGEYDLSGWKNYKDQVFQSVQNISVKWSALRIQSPQEQVWKVTFRQIYQGDGYRDI
                     GLKTLTLKGFPGNIKIIGEEWVEEK"
     gene            complement(35875..37173)
                     /locus_tag="PKX09_04325"
     CDS             complement(35875..37173)
                     /locus_tag="PKX09_04325"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOU17080.1"
                     /translation="MSSSDTADLRPILNYLFSLNGRGIKPGLERIQKLLDAIGNPQNA
                     YKVIHVAGTNGKGSTCAIMANVLQAAGYRVGLYTSPHLLNFNERVRIDGQKIPDAEIA
                     LFTESWRKLIDELNCSFFEATTAMALDFFKKNKVDYAVLETGMGGRFDATNVTLPEVT
                     VITPIGRDHQEFLGRRLAQIAYEKAGIVKSGIPCIVARQTPRVLNFLSKEINASGGDF
                     YYAPEQCQITNIQRFPERQVLSLIVNNLKIDTVKFPLIGDHQLINLQAAVSALMRVKD
                     LELSAEVLRQGITGVIWPGRLHILSHKPLIYFDVGHNQHGIRQVVKALKQIFPTQPVH
                     LVLALGSVKKYDRIGKIIQPLGGDIYLTEIPDHPSVSAEILADVIRKQIPSRLIYVDN
                     KLERLLNFLEIQIKSDEILLIAGSHYLAPQVLPFYGVSLT"
     gene            complement(37173..38009)
                     /gene="murI"
                     /locus_tag="PKX09_04330"
     CDS             complement(37173..38009)
                     /gene="murI"
                     /locus_tag="PKX09_04330"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOU17081.1"
                     /translation="MTNQWLKVSNKRNLPIGVFDSGLGGLTVVKQLFRQLPQEQIYYF
                     GDTARVPYGNKSVETVQKFAIQIARFLAERSVKMIVVACNTASSVALELLQDQFDLPI
                     IGVIEPGARSALAISPAKKIGVIGTTGTINSGKYVEVLTALDKSVQVFSQACPLFVPL
                     VEEGWGESPVTEIVAREYLQNLVLKIDTLILGCTHYPIIKNVIQRVVDSNIQIIDTAI
                     ETANQVRLILNQQGLLNDLERTPEHRFYVSDLPQKFEEIAMRFLGEPIANIERINLAD
                     CC"
     gene            complement(37981..39561)
                     /locus_tag="PKX09_04335"
     CDS             complement(37981..39561)
                     /locus_tag="PKX09_04335"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOU17082.1"
                     /translation="MKIFRPYIYFLLFLVNCHNLLADQLVIQSPHPQAGTEISIIYFN
                     DCAYFSTKDFAQALGLRTYNNPETGKIVLYFSGKSVKITAHSAFVMLDTRILQMSQPA
                     ILVNRDEIFVPLYSFLDLMKRYVIPKFQYHLDRSSSIYELVELPGATQAPSASTVTTF
                     GTKPGIIYDIQFEERGNGLTMKILTNAPYESSDFSSFFRGDEWFYLTILGATCDSAAL
                     SRRDPIPSVAIVEAIPVGNSVQITLKLKQKYDRANAYYDARSGKILVTLNVPLTSATL
                     KKIEEVKNTWIVDTIVIDAGHGGKDSGTPGRWGYKHEKDIVLDIALRLGKLLEQRDDL
                     KVIYTRKNDEFIPLWKRTRLANDSAGKLFISLHINALPSGTKGNAEGIELYLQNPTIR
                     TQESIEVARLENEVIKLETSEDKAKYKDYDNPSHILANMVYNTYLHDSEKFAEILSRN
                     ISEYVPQKNRGVKQANFYVLVGASMPNLLCELGYNDNRNDAKKLNDPAHRQKIALALY
                     KSVIEFKEYCDKSVAQSK"
     gene            complement(39590..>41213)
                     /locus_tag="PKX09_04340"
     CDS             complement(39590..>41213)
                     /locus_tag="PKX09_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17083.1"
                     /translation="SQLIELKRHYPKSLNLKALVKDTIFTTAEIEAISYFIDFSVKRT
                     KPSQIVNFISLKKQEYTSLQKSLQRARCYSPQGWFTGIIVENDSDEPILGDYWNISLR
                     SPKYFNSLEILGGAYRLQWGQGLLFSNNLMSGRSTDATGNLKTSRSGVSNYLGSDENR
                     YLFGSAAQYTSGHFKLIPFISHHRLDATIDTNGTVKNLRSDGLHISSSQLRAKDALLE
                     TLGGASLLGEWDQGSAGILVYNANYSTDLFALNGHRKVSGLSFLHNYEISDWTISGEW
                     AVQETGKKGIIQNLHLSRDRVSFCAGWRYIEPDFFAPLGNPFRKFSSLSGNETGLYSA
                     LKIKLPARWRFTGYVDFFRELKNSEPGVAIRNGRESLCGFSRQARNNDLIEAWYKVNR
                     YYHTSLPQQPRDYQLKLHLRHSFSSSFAGEIRTTFRWNDSIPFTEGSQALSIVGKVSI
                     SKYTRVTAGNTHYFTGSSDLNIYFYEPGLPFRFNMTLLTGTGQRYFLIISRRIGSNCE
                     IAGAVYGRSNVSIHTNDNRYDFSGDLQLTFDL"
BASE COUNT        11618 a         8948 c         8787 g        11660 t
ORIGIN      
        1 tctatcccgc agttacatac ggtagattat cctacctggt taaaaatcgc ctcaattggt
       61 caattccggt acattcccaa accactttcc acctggcgcc gtcattctca acaattaacc
      121 aggcaacgaa ttgtaccttt gacaaaggct atgattcctt ttttattaaa aacacaagat
      181 tcacttcccc cccaaattgc tcggaatgtc aatatatcaa cttctcaatt gcagcactat
      241 tgggaaaaaa tgatgactat tattttaatt cgaggaggac gatataaatt aatagtaaga
      301 gactgggaaa cagccagaca gtactactgg gaatcgttca gaaaatggcc tgcattcata
      361 cctttttgga gagttaaagc tatactcggc atattgatgt cctattttca tggtaatatg
      421 gagtggacaa catctatcag agtagctttt tcaaaaatta aaagcaaaat tgtcagaaaa
      481 gcatagtgat tgatctaatt aagacaagca taaaagactc ttttatttac agctttggca
      541 atattatgct acgaatctcg ggattcattc ttttgccagt gtatcttact caactctcac
      601 ctgctgaata tggaatttta ggattattag aagtaacgac ccaggtattg gttcagatct
      661 tcagtctagc gatgcatcta ccactaattc ggtggtattg ggctcctgaa tatcacaata
      721 aacaaaaaac aatttttttt tctacgataa tgttttcgag cttgctagct gggctaatgc
      781 taataatttt tttaccatgg gcttcgactt ttgctcaatt tttattccat gatacaaatt
      841 acgctaatct aatacagctc atgctagtta gcgctagttt gcagattatt acaatgatcc
      901 cgactactct ggcaagagtg cagggtaaag caatacttta taccatttct catattttaa
      961 actttattgt tagtttatta ttaactctat tttttatctt aaaattggat atgaaggtcg
     1021 agggcattta tcttgcccaa attttaggtt atatagtaaa tattttattc ctaggacgat
     1081 ttatctggca aaactcagaa ttgaattttg atttcacaat attaaaagaa atgctgaatt
     1141 atagtttccc attagcacta tcatctatgg catcagtctt attgacaatt gctgatcgtt
     1201 atacattaaa tattctaggg cagttatccg acgttggtgt atattcgctt ggttttaaaa
     1261 ttagcaatac tttaaatgtt ttagttatta gttcgataaa tcttgcactt actccgctaa
     1321 tttatcaaaa aatggaggat ccaaataata agcgatttta ctcaaaaatc atgacttata
     1381 tgactttcgg aataatgttt tttgtattag ggttgtcgct ctatagccaa gaaatagtac
     1441 agttattcgc caaagataaa gcttattggc ctgcctatcg ccttgttcct attctttgct
     1501 ttgcaattgt ttttggaatg ctaaaagacc tttctactat tggcttgaac cttaagaaga
     1561 aaacaaaaat tattggattg atagtaacta taatatcatt tatcaatgtt ggattgaatt
     1621 ttctttttgt ttcacttctt aagactactg gtgcagccct ggcaatgtta ttgacccagg
     1681 taatcttttt ttttgtaatg tttcaagccg cacaaaagca gtatcgtata ccctatgaaa
     1741 ttcaaaaaat atttaaaatg cttttattag gaggcttctt aatcggtata ggattctttt
     1801 tattcaatga aaatgagcca ttgattgtac gaatattttc taaacttttt ctaataatac
     1861 ttttcccgat tggactttat ctctggaatt tctacgagcc tattgagatt caaaccatca
     1921 aaggcgcctg gcgcaaatgg cgtaatcctt taaattggaa aaagaaccgt tctaagatta
     1981 aattagaccg taaataatga gggttgaatt atgagtaaaa aaaccaaaaa gtcaacatct
     2041 gccaaaaatg tccctccgca aaataagaag ccggttaact ggctagctac gattaatccc
     2101 cgaaaggcga caatcgtagc atttcttatc gtattattga tattaattat tttttataag
     2161 ccctatgtaa ttgatcgtct tgaaccggct ggtggcgatc ggatggcaag cattgcccag
     2221 agtaaattta tcaacgatta tcgtgaacaa actggtgaaa ttccactctg gaatcccgcc
     2281 attttttgtg gagtaccgat ttattatgct ctctggtcta attcattcaa tctggattat
     2341 ttaatcagta aaattagtaa ggtaattgat tggaaattga gttggttcat tttggcaact
     2401 gtgggaatgt ttttaatatt ttggttgctg gaattcccgt ggtattatgc cttgcttgga
     2461 acgattgcct ttctctttta tccacattat caggcgctga tcaccgtcgg tcattttgcc
     2521 aaagtgcgag cggtttgcgc gatgccgtta gccgtgtttg gctttttata tttagtcaag
     2581 aagcgaaatt ttctgtcatt tttactgttt gtgatctttt tctcactcca attgcgtacc
     2641 cagcattatc agatagtctt ttatacgcta ttagttttta tggctctggg catacggcaa
     2701 attgttgaat gggtcaagac caaacaagcc cagaaaattt acatttccct cggattattc
     2761 gtcgccggat tggtgacctc agttttaatg tcagcccagc cgttatttgt ggtgaatgaa
     2821 tatacaccct attccactcg cggcggccaa gccattaatc taaaggaaga tgcggctcag
     2881 gcagaagtaa agagtagcgg cgtgactttc gaatatgcga cgcgctggtc gcttaatccc
     2941 aaagaattag ctaccctgat tgtcccgcgt ttttatggcg gcacatctca ggaaccctac
     3001 accggcaaag cttatccgca actgcggggt cagcctattc cgggttattg gggcgatatg
     3061 ccgtttacac aatcatgtga gtatatggga attttgatcg tgattctagc tttgctgggc
     3121 ttgtggtatt atcgcaaaga tggggtggta attagtttat ttatcctgct ggtattttcg
     3181 ctactgctgg gtttcggcag tcattttcca attttataca aaccactttt taagtattta
     3241 ccctatttta gcaagtttag agttccatca atgattttga ttctggttga tttcattcta
     3301 gtaattctct cgctttatgg attgaaagga ctggtagaaa aatttgattt agccaaatat
     3361 aaatcggctc taatggtcag cggctttttt atattgttcg gtttattctt tttactggcg
     3421 ccgggagtgc tgtcgtacac ttccgctcag gacgctcaat atatgaacaa tccccaagtc
     3481 attgaaatgt tgcgcacggc gcggcgtgaa tttttacagg cggatactct gcggatgctg
     3541 gtttttgtaa tttgttttgg agctctggtg gtgctatata aaatgaaaaa gattcgcccg
     3601 gattttctaa ttggcgcgat tatcattctg gtcgccgtag atctgattaa tgtcagtaag
     3661 cgtttcttga gagctgacga attagtggat accaaatccc tcgagcgacg ttattttgcc
     3721 gaaaccagct tcgataaaat tctcaaacag gatcaggaga tttttcggat tctaggattg
     3781 ggtaatcacc ttcaaagcaa cgatttagcc tatcgctatc aaatcgttac gggctattca
     3841 ccgattaagc cgcaattgat tcaagatcta attgataata atttagttgc gggaccgact
     3901 cagagttcgt tgaactggaa tgtaatcaat atgctaaatg ctaaatatat tattgcgccc
     3961 ggtatgttga atgaacctaa tttgacgatt ttggatgtta atcagcaacg gaaagaagtg
     4021 ctttatttaa atgaaggcgt ccttccgcgg gcttatttcg tatctgaagt caggttttta
     4081 ccatcggaaa aggatgtagt agcttttatg aacacgaccg agtttgatcc ggctaaaatg
     4141 gctttgacga gtgtagcgct tgatacttcc gcaggatttg atactggagg aagcgttcaa
     4201 gtaaccgatt atactcccaa tcgcgtggtg ctaaacgtcg aaaccgagcg tccggccttc
     4261 ttggttctgg ctgatgctta ctatccgaaa ggttggacgg ctcgcgtcaa tggcgtcgaa
     4321 acaccgattt atcaagtcaa tcatgttttg cgaggagtgt cggttccagc tggaaattat
     4381 gcattggaat ttaagttttt accgcgtagt tatcagatag catcccagat ttctactatt
     4441 tcttgcgaca tcgtctggct tagcctgctg ggagtgttga tttatcaaaa tcgggagaag
     4501 attaagaacc ttaagaaaaa gcgcccaaca cctgccaaaa gcaaccgatg attttaccaa
     4561 tgcaactaaa atgattatta ttggcatagt tccatgaaaa taaatattta tcgtcattct
     4621 ggcaatatca agcgcttgct gttagttctg gcaattgtta tgattttcgc cttgctgaat
     4681 tatacccaga aaatcgtcac gcgcctgcgc caggattccg ccaatttgat ccggttttat
     4741 gctgatattt atgccaaagc cgctatggat atgagtggcg acgactttag ttttattttt
     4801 gaagaaataa tccagaaaac ctcgactcca atgattcttt cggatgccga ggctatggaa
     4861 ccaaccgcct ggcgcaacat tggcgttccg gataacgatc gttcgcccgc tactctggct
     4921 aaagtcaaga agataatgca agaaatggat cgggaaaatc agccaattcc actgaaatac
     4981 gaagatcgag tattgcaata tattcatttc ggcgatacca atatgattcg ccaactgcgg
     5041 cgcttgccct atatcgaaat tgccatcgtc gggctgttta ttttgctcgg atatgtcggc
     5101 ttttcgttaa tccgggaaaa tgagaagcgc tcaatctggg ttggtatggc aaaagaaacc
     5161 gcgcatcaat taggcacgcc gttgaccgcg ctgatgggtt ggttggaatt gctgaaggat
     5221 gaaatcggtg acaatgccaa tatcagcgaa attgcgcgcg acattcagcg gttggaaaaa
     5281 gtggcaaatc gtttttcgca gatcggcagt cgcccggttc taaaaccgac taacattaat
     5341 tcggttatta acgatgccgt ttcgtattat cggcgccgtt taccacaact gggaccgggc
     5401 attagtctgg aatttatccc atctggcgat tacatcgtca acctgaatgc ggatttatnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag tctggaattt atcccatctg
     5581 gcgattacat cgtcaacctg aatgcggatt tattttcctg ggcgctggaa aatttgatca
     5641 agaacgctat tgacgccgta tcgcaggaga acggcaaaat aagagtagaa ttacacctcc
     5701 ttcatggcgg gaagaagatt gccgttgatg tcattgataa cggcaaggga atcagtaaga
     5761 ataaccgcaa gaatattttc cgtccaggct acagcactaa gcaacgcggc tggggtttag
     5821 gtctgagtct ggcaatgcgg atagtgaagg aataccaccg cggacagtta ttcgtcttga
     5881 tgagcaagcc caacgaaaaa accgttatga gaatcgtgct gaaaaatcca caggaaaact
     5941 tgaatagagg ctaagaaata gctttttaaa cctagtgaaa caagccttta aagatctttt
     6001 tgttataagc gaatctcggc tgatcgcaaa acctataatc agctataaat gattctccaa
     6061 agtggacatc gtgtttctca gtcagtcagt gaattataaa aaatacttgc attttttcca
     6121 aaacatccgt aaatattcgc ccgtatggaa aaaatcagca cattagaaaa tcaacaaaat
     6181 accctgaact ggtggtggca aattagtaat ggggcggtga gatgctgaaa taagcgttta
     6241 agcaactctg tattggcata gacccgcccg aaaaaaaggc gggttttttt gttttattga
     6301 caataacgaa aaaataacaa agaaaggaaa tgaaaatgaa gcaacacaaa ttcaccttgt
     6361 ctgaatccga aatgccaaaa gcctggtata acattgcggc tgacctgccc tatgctctac
     6421 caccggcttt acatcctggt acccaaaaac ctattacgcc cgatgacttg gcacctatct
     6481 ttccaatgtc tcttatcgaa caggaagtga gtacggagcg ttttattccg ataccggagt
     6541 cggtactcga gatttatcag ctatggaggc caacgccgtt ggtgcgggct tatcggttgg
     6601 aaaaagccat cggcacacac tccaaaattt atttcaaaaa tgagagcgtg agcccgcccg
     6661 gcagtcacaa acccaatacc gcagtcgcgc aagcttatta caattacaaa gaaggagtca
     6721 aacgcttgac caccgaaact ggcgccggac aatggggtag tgcgctggct tttgcctgca
     6781 attactttgg tctggaatgc gttgtattta tggtgcgcat cagttacgaa accaaaccat
     6841 accgtaaatc tatgatcgag acctggggcg gtcgtatttt cgccagtccg agtcaagaaa
     6901 ctgccagcgg acgagcgatt ctcgctaatg atcctaattg cgccggtagt ctgggaatgg
     6961 caatttccga agcggttgaa gttgctatga atgataaaaa taccaagtac tcgctcggct
     7021 cagtactaaa tcacgttatg ctccatcaaa ctattatcgg agaagaaact cagaagcaaa
     7081 tagcccagac cggtgattac cctgatatta ttatcggttg cgtcggcggc ggcagtaatt
     7141 ttgccggttt ttcgttcccg tttattcggg ataaaattgc tgggaaaaaa ttgcgagcta
     7201 tcgcggtcga accgactgcc tgtccgtcgt tgaccaaggg tctatattct tatgattttg
     7261 gcgatacggc taaattgacg ccattgttag cgatgtacac gctcggtcat aatttcatgc
     7321 cctcaaaaat ccacgccggt gggctacgct atcatggcgc ttcaccattg gttagctcga
     7381 tggtaaaaga aggcttgata gaagcgcgct cctatcaaca aactgaagtg ttcaaagccg
     7441 cggttacttt tgcccggagc gaaggtatca tccccgcacc ggaaaccgct catgccatca
     7501 aagccactat tgatgaagcc aagaaggctg atgaggaagg tgttaccaag acgattatgt
     7561 tcaacttcag tggtcatggt cacttcgata tgagcgctta cgattcctac ttcgccgggc
     7621 gattggaaga caaccatttt tcagatgcct cactgcaaaa gaacctagct gagcttgctg
     7681 atttgcccaa aatctaagag taaaattact aggttatcga acggggcggg ataaatcccg
     7741 ccccgcttgt ctgtcactga aactctgggc tggtttgaac aattaaagtt ttttacattc
     7801 tactcttacg atgaaagtct tatattcaaa acctcaagtg aaaactatcc agattgatgg
     7861 ccttggttca gttaccctgc gtcgcgacca ccgcgcccga cgactaactc tgaaagttaa
     7921 accggatcag cagatcacgc ttactgttcc atattcaatt agtgaaaaaa ctgctcttgc
     7981 cttcctagaa gccaaccgcg aatgggcttt aaaaaatatt caggctctga aggaaattaa
     8041 accacctatt ttcatcatag aagacaaaca aaccatccaa actcgtactc accgtttggc
     8101 attgcaaccg actagtggtg acgaaattag agtcggcatc cagcctggaa ttattaatgt
     8161 tcaatatccg tctgatcaaa aggttagcca tccacgaatt caagaggcga ttagatgggg
     8221 actgacagcc gcttatcgca tcgaggctaa acaattttta cctccaagag tgcgcgattt
     8281 agcccaacgg tacggttttc gttacaaccg actttttatt aaaaacctca agtcacgctg
     8341 gggcagttgc tcggccgcca ataatattaa cctcaacctg caccttatgc gtttgccgga
     8401 tgagttgatt gattatgtga ttctacatga attgacccat acccaaatca agaatcatag
     8461 tccgcttttc tggcaggaac ttgagcggat gctgccagat gctaaagtac gacgccagca
     8521 attgaaaaca atcacagtcc agggattttt accgtcggtt gtaatggaga attaattttt
     8581 caagaaataa ttatttaaaa acaatgatat tagccatcat attaaaaaat actaaaatta
     8641 ttgttgttaa tttcaaaaag gattttataa tttccaatta tgtataaatc tcaaaaaaca
     8701 tctctcaaaa gcgaagatca agatagtaaa tctggtgcta atccatttcc tgattgtctc
     8761 gacactgccg actggcggaa actgactgct ctccagaacc aatatgtcga agcaattgtt
     8821 accgagtata tccgactttg ccgaccagct aaggttagta tcctcagtga cactcctgag
     8881 gagattgcct atgtgcgaca attggcgctc cgtaccggcg aagaacagcc gcttaaaatt
     8941 gccgggcata ccgttcattt tgacggttat aatgatcaag gccgtgacaa ggaacatact
     9001 cgcattttag ttaatgatgg gcatttgaac agcgaacgcc ttaatactct cgatcgggtc
     9061 aaaggcttaa aagaaattca tcaattgtta gatggtatca tggctggtaa agaaatgctg
     9121 gtacgctttt attgtctcgg tccgctcggg tcaatctttt ccattccggc tttacaaatt
     9181 accgactcat cttatgtcgc tcatagtgaa gatctactat atcggcaagg ttatagcgaa
     9241 ttccgacgac taaatggctc tgatcggttt tttcatttta ttcatagtgc cggagaactc
     9301 gatgagcgcg gctgttcgaa aaatgttgat aagcgtcgga tatacattga tttggaaagt
     9361 aaccgcgtat ttacgattaa taatcaatat gccggtaata gcgtgggttt aaaaaagttg
     9421 gctctccgtc tggcaatcaa taaagccaat cacgaagatt ggctggctga acatatgttc
     9481 attatgggag ttcatcctct acaaaaaaaa cgcaccacct attttactgg tgcatttccg
     9541 agcgcttgcg gcaagaccag cactgccatg ttgcctggtc agacgatcgt cggtgacgat
     9601 atagcttatt tgcgtgtttc aaatgataaa aaatgcaatg ctgttaatat cgaatccggt
     9661 atttttggca ttattgccga tgtcaaccct atcgatgatg ctttgattta ccgttgtttg
     9721 acaacgccac gtgagctgat tttttcaaat gtactggtaa aagacggaaa accttactgg
     9781 ctgggaatgg gttgcgagac gccggaaagc ggtatcaatc attccgggga atggttcaaa
     9841 ggcaaaaaag atgcggctgg gaaagaaatt ccgctggctc ataaaaatgc gcgttatact
     9901 atgcgtctca gcgagctgga aaatctcgat ccacgcgccg atgatccgaa cggtgtgccc
     9961 atttcagcga tcatttatgg cggacgcgat tcagatacca atccgccgat tctgcaggcg
    10021 cttgattggc ttcatggcgt tatgctgggt gcgactctgg aatcggaaac caccgccgct
    10081 actctgggac aagaaggaca gcgcaccttc tctccaatgg ctaatttgga tttcgttgtc
    10141 gtgccgctcg gactttacat tgagaatcat ttgaaattcg gagcgcgctt agcgaaagcg
    10201 ccacttgttt tttcaaccaa ttatttctta aaagatagtt ctggtaatta tttgaacgaa
    10261 aagactgata aaaaagtctg gattttatgg gctgagggtc gcgtgcataa cgaatatgac
    10321 gctatcgcga cgccagtcgg ctatctgcct caatataatg acctacaaaa cttatttaaa
    10381 ctggcactcg gcaaagacta cagtcgaacg gcttatgaac agcagttcac tttacgtatt
    10441 gataaattca ttgccaaact ggatcgggtc agcgaatttt atcggaatga aaccgttccg
    10501 gctgaattca acaatcggct ggctcagcaa cgggctgatc tggtcaaatt gcaggaagag
    10561 tatcaagcgc cagaaatcag tccattccaa attgtaaaaa gataaaaaat caattaatga
    10621 gcacctagca aaaatgcctt gcaattaaaa ggcaaggcgg tttaaatttg cttgcttttt
    10681 tagcgttaac gaatgaaatc aaaggaatta aaaaatgaaa cccaatattc acccagaata
    10741 taaagaatgc accgttactt gtgcttgtgg taatactttc gtgacacgct cgactgtcgg
    10801 cgatctcaaa gttgaaattt gctccgcctg ccacccgttt tttaccggca agcagaaatt
    10861 gattgacagt gccgggcgca tcgaaaaatt taatcgtaaa tacaagcgcg atacccagaa
    10921 aggttagctt cttttaacaa gattaacgac ttcttcacag cagtgaagag gtcgttttta
    10981 tttgtatatg gcacagaaaa tattagtcgg cgggcaagca gtgatcgagg gcgtgatgat
    11041 gcgcgtgccg ggagcttatg ccacggcggt gcgccgaccg gacggcgaaa tcgagattaa
    11101 acgagaaaat tttacatcca ttgccgaccg cattccattt tttaaaaaac cgctcctgcg
    11161 gggcataatt agtatgtttg aatcgctgaa aattggcctg gcaacgattc aactctccgc
    11221 cgatattgct atgcaggcgg aaaatgccgc caaaccggcg gataaaacca aggatagaat
    11281 tgccactacg atttcaatga ttttagcctt tggattagcc tttttactgt ttggattttt
    11341 gccactgtgg ctgacgaccg agttgttgaa tattgagcgt aaggcactgg cttttaacct
    11401 tgtcgttgga gcctggcgca tccttttttt cctaatgtat ctctggctga tttcatttat
    11461 gaaagatatt cagcggctgt ttcaatacca cggggctgaa cacaaggtcg tttttacttt
    11521 tgaaagcggg caagatctga cggtcgaaaa tgctcagcca tttaaaactt atcatccgcg
    11581 gtgcggcacg agttttatct tcatcgtaat gatggttggc attttgcttt ttgctttgat
    11641 agatgcggtg gtaatctggg gctttggttc gatttcgctg gtcgcccgct tgatttatca
    11701 tcttgcatta ttacccttag tagccggaat cggatatgag tttttaaagt tatctgccaa
    11761 attccagcat aaacgtttta cgcaatggct gattgcgcct ggactttggt tacagcgaat
    11821 tacgactaag ccgccgaccg atgaccagat cgaagtcgct atcgcggctt taaaaggcgc
    11881 gttcggtgag cattatgccg agttcgctgg ccgggaatac atagccgaag cggtagatta
    11941 aaagatgcgc gaacagattc agacaattat caaacgttat aatgacatca cggatgaaat
    12001 gtcgcggcca gaagttatcg ccgaccagca tcgctaccgc gaattagcca aagagcataa
    12061 agatttaaca ccgattgtgg acacgggtaa tcattatttg caggtgttga aaaacattga
    12121 cgatgacaag gcgattctcg aaggtcatga tgaagatttg aagcaactgg cgcacgaaga
    12181 gctcgctact cttgaagagg aaaagtcgcg tttatttgag gagttaaaaa aacttttaat
    12241 tccgaaagat cccaacgatg ataagaatgc aattgtggag attcgagccg gcacaggtgg
    12301 tgaagaggcg gctatcttcg cagctgatct ctatcgtctt tatttaaaat ttgccgagcg
    12361 caaaggctgg aaagttgaag aactttccgg cactcagaac ggtttgggcg gctttaaaga
    12421 ggtaattttt ttattgtccg gcgaaaatgt ctatgggacg atgaaatttg aatcaggtgt
    12481 gcatcgcgtc cagcgcgtgc cggtcaccga aacacaagga cggattcaca cctcagcggc
    12541 tacggtggcg gttctgccgg aggctgagga agttgatatt gaaattaatg ccagtgaact
    12601 gcgcatcgac acttatcgtt ccagcggcgc tggcggtcag cacgtgaaca aagtcgagac
    12661 cgctattcga ataactcacc tgccgactgg cttggtagtc acgtgtcaag acgaaaaatc
    12721 acagtataag aataaagaga aagccttgaa aattttgcga agccgtttac tggcgcttaa
    12781 agaagctgaa aaggaagccg aaatcgccgc caaacggcgc agtatggtca gtaccggcga
    12841 tcgcagtgcc aaaattcgta cttataattt tccgcaaggg cgcgttaccg atcaccgcat
    12901 caatttgacc ctttatcgct tgacggaagt aatggaaggt gaaattgatg aattgatctc
    12961 cgctctgcaa ttggctcaca ataccgagct tatgaagagt tgaccaccac gaaaagtttt
    13021 ccgcgctgac gattcgctaa aaacattttc tattttaccc caaaatctgc ttgctcggct
    13081 ccaaaacaat cttaaatttc cataaatact atggcagaaa gagaacggca aatttggcga
    13141 atagtagatg tgctaaagtg gtcgagagac tacctgaaag gtaagcagat cgaatcaccc
    13201 cagatcgaag ccgaatggat tttgcgagaa gttctaaact ttaaccgtct ggaaatatat
    13261 ctgaaatatg agcgcccgct ttctccggcc gagttagccc gcatcaaggc gctccttttg
    13321 aagcgggcta gcgggcaacc cattcagtat gtactcggaa gtgccgaatt tatgggcttg
    13381 aaactaaggg tgacgccgga tgttctgatt ccccgaccgg aaaccgaact gttggttgaa
    13441 aaaaccctcg aattgtgtcg taatcgcgtg acggcgcgca ttctcgatat tggtaccgga
    13501 tcggggtgca ttgcgattgc cttagcccat tttttgccgg aatgtcaggt tgtagctatt
    13561 gatgtaagtg cgacggcgtt gcaaatcgcc cgcgaaaatg ccgccatcca taatgtggct
    13621 aatcgcattg atttccaaga actcgatatt cttcactcac ctgagttaac gatgggacaa
    13681 ttcgatatta ttgtttctaa tccgccttat gtctccgggg attactatgt aaatctgccg
    13741 aaattagtca aggagcacga gccggaaatt gccctgaatc ccggaacaga cggcttgctt
    13801 ttttaccggc gcatcaggga attagcgtct aaaattgcga ttcccggtgg ggcagtcgtg
    13861 ctggagatcg gcggagatta tcaggcgacg gctgtggctg aacttttttc cgagagtgct
    13921 tttgattcgg tcgcaatcgt taaagattat agcgggcaaa gtcgggtaat catagcggag
    13981 ctggcgaaat gaaaaatctg cttgcctatt ttaaaattat gcgtcctttg aatttactac
    14041 agggaggaat tgccattttg gtctgtatgg cattggctga tccaaaaccg gaatggctga
    14101 gtgtcttgct ggctctggtc attgtctggg cttataccgg agccggcaac atcctgaacg
    14161 actattttga cgtcgaaatt gatcgcatca accgccccca gcgaccgatt ccggctggct
    14221 taattcagcc acgcaccgca ttattttatg ctatcttttt attcgccttg ggaacggccg
    14281 ccgtagttcc ggttttcaat cgccagttag caatcatcat ccctttcgcc ttattttttc
    14341 taataaccta cactaaattt ttcaaaccgc gtccgttatg ggggaatctg attgtatcgg
    14401 cgattctcgg aatgacgttt ttattcggtt catccatttt tggtgatatt cgggtaggga
    14461 ttgttccttg tttgctggct tttggtttta attttgtgcg agaaattgtc aaggatatgc
    14521 aggacgttgc cggcgatcaa gctcgggggg cgcggacttt tcctctggtt tacggattaa
    14581 ccgccgcccg tcgtctggta attgtggcta cgctgattct tatggttggc gcgttagttc
    14641 ctttttatct gaaaatatac ggaaagtttt atttgctgat tctggttttg acagttgaaa
    14701 tcccgctgat ttatgttata ttttcaatta gtcgcgatgc ttccgcgacc aattgcggcc
    14761 gtatagcggc gattttgaaa ggtgatatat tttttggcct gctggctatt tttgcaggga
    14821 aattttaaat gacacaattt gttcatctcc acaatcattc ggattatagt ctattggacg
    14881 gagctcaatc tatcgaatct ctggtaaatc gggcagtcga gctgagtatg ccagctgttg
    14941 gcttgaccga acacggcaac ctcttcagcg cgattcattt ccttaaagcc gccaatacaa
    15001 gtggcttgaa gccgatcctc ggatgcgaga tttatgtggc ggataataac cgttttgata
    15061 aaaaatcgcg cgaacaaggt ggtagcggct atcatcatct actactatta gttcagaatc
    15121 gccagggata tgagaacctg attaagttag tcagtcttgg ctacctggag ggatattatt
    15181 atcggccaag agtggacaat gaacttctgc gaaagtataa tgccggactg attgctacca
    15241 gcgcctgtat caaagggcgc attccggatt tagccatcaa aggcgattat gaaaaagcca
    15301 agcagttggc gctggaatac gctgaaattt tcgctggtcg cttctatctg gaagtgcaaa
    15361 accatggcat tgatgatgaa ctgcgttggt acgaaattgc taaaaaattg tccgctgaaa
    15421 tcggtctgcc gcgcctgcta accaacgata ctcattatac tttaccggaa cattgggaag
    15481 ctcacgatgc ccatctttgc attggcaccg gcaaagaaat gagcgatcct gaccggatga
    15541 aatacgaaca acatgagtat tgggttaaat cggctgaaga gatggcggct ctttatccca
    15601 acgatcctga gataatcgaa aatacgctga aaattgccga acagtgcgat tttaatctgg
    15661 agatgaacca atattatctg ccggaatttc ctatcgcgga atccgctaag gtaaaaactg
    15721 ccgatgaata tctggctcaa ctgatcgagg aacgacttcc aggtcgctat ccagaaatta
    15781 caccagaaat tcaggagaga gtggattatg agttgggagt aatccgccaa atgggtttcg
    15841 ctggttattt tctgattgtc caagattttg tgcattatgc taaaaataat aacatcccgg
    15901 tcggaccggg gcgtggttca gccgctggca gtatcgtggc atatatttta ggcattacta
    15961 atattgatcc cctaaaatat aaacttatat tcgagcgttt cctaaatccg gaacgcatct
    16021 ctatgccgga tattgacatt gatttttgcg ataccaaacg cggcgccgtt attgattata
    16081 ttcgcaagaa atatggcgag aactgtgtgg ctcagatcat tactttcggg aaaatgaaag
    16141 caagagcggt gattcgcgat gtcggacggg ttctcagtat gccgctggcg gaagttaacc
    16201 gaatcgctaa actggtccca gaacaaatta atatcactct tgaaaccgcc attaacaata
    16261 ctccggaact gcaggaagcc gtcaatattg atgaacagca tcagaaattg tttgcaattt
    16321 ccaaggtttt ggaaggcttg aaccgtcacg cttccaccca tgccgccgga gtcgtaattg
    16381 ccccgggcaa tctgacggat tacgttccac tttaccagtc aagcaatggc gaagtcacca
    16441 cccagtatga tatgaaatgt attgatcaga tcggcttgct caaggttgat ttgttgggaa
    16501 tccgcaacct cagcgttatt gacaatacac tcgcgatgtt aaaagctaag ggcattgaac
    16561 tcgatataga taagattccg gaaaaggatg aagaaaccct tgctcttttc ggcgaaggcc
    16621 gcacgatcgg cattttccag tttgagtctt ccggaatgcg ggagtacctg aagaagctga
    16681 aaccgtccgg catcgaagac ttgatcgcaa tgaatgcgct ctaccgaccg ggacctatgg
    16741 aaatgatcaa tgactttatt gatcgcaagc aaggtcgcaa accaattact tatttgcatc
    16801 ctttgctgga accgattctc gtggatacct acggtattat cgtttatcag gaacaggtta
    16861 tgcaaattgc ccatcaggta gccggcttct cgctggctaa agccgatttg atgcgcaaag
    16921 ctatgggcaa gaagcagaaa aaaactatgg aatcccttaa aaaggaattc gttgaaggtg
    16981 cgatcaaaaa cggtatcaag cgttcggttg ccgtccagat ttatgaatta attcagcgtt
    17041 tcgccgagta tggattcaat cgcagtcatt ctgccgctta tgccattctc gcctatcagt
    17101 gcggctattt gaaagctcac tatccggctg aatttatggc ggctaacctt tcgagtgaaa
    17161 tcaatgacac cgaccgaatc acgattcttg ccaatgaagc ccgcaatatg ggttacgaaa
    17221 tcttaccgcc cgatgtgaat tatagcgaag ttcgcttcat tccggaaggg aaagcgatcc
    17281 ggtatggttt gcatgctatc aagaacgtcg gcgagaaagc cgctgaaaat atcgtcgcca
    17341 cccgccagac cgaaaagcaa tttcgtactc tttttcaaat ggttgcggct ctggatttga
    17401 aaagtgtcaa tcgtaaagtt ctcgaaagct taattgcggc cggtgcaatg gactcgctgg
    17461 aaggcactcg cggtcagaaa tttgccgcca ttgacactgc tattcaatac gctacgcata
    17521 ttcaagaaga aaaaaacaat aatcaggtga atctttttgg aatcgctaac ggtgcgccgc
    17581 aggctacttt gagtgaaccg cgtttaccgg atgtagctcc ctgggaggat tccgaaaagt
    17641 ggaataagga aaaagaactg ctcggtatgt atttaagtgg gcatccgcta ttggagtatt
    17701 cgacggaaat agaatccttt tccaattacg atttcaccga gccgctatcc aatctggata
    17761 agtccaatgt caaattaggc ggactgattg ccagcatcaa aactatgacc gatcggaaag
    17821 gtcgcccgat ggcatttatc aatcttgaaa gcctgaacgg caaggtcgaa ttaatcgcct
    17881 tcgccgatat ttatgaaaaa tatcgcgaac atctcaaaat taatcatccc atctttgtca
    17941 gcggaaatgt caattgccgg aatgaagacg acgccaagat tatcgtcgag gaaatcagcc
    18001 ccttgcgcgg tttgcttaac cgcaaatcca aaaaacttca tgtgcgcatc aatgtcaatc
    18061 aatttggtgc tgaagatctg gaaaatctca aaatattact tgcgaaacat accggcgagt
    18121 gtcagctgtt cctgcatctc tatgacggta atggcgtcaa caaatcaatc cgttcgggaa
    18181 atctgagagt tagcgccgat cgcgaactgc tcgatattct aaagaaaact tttggcgaag
    18241 aaaacgcctg ggtggaataa tgatcgcagt tattcaaaga gtcagccaag cccacgtgac
    18301 cgtcgaaaac aaaattgtcg gccggattgg gcccggatta gtgattctac tgggtgtttt
    18361 tcagaatgat ggtgatgcag atgccgcttt tctagctgat aaaattgctg gtttgcggat
    18421 ttttggggat gaacagcaaa aaatgaatct ctcaatcaag gatgttggtg gcgcggcatt
    18481 ggttatttcg caatttacct tatgcggcga ttggcgcaaa ggccgccggc caagctttat
    18541 ctcagccgct gatccagaaa ccggacggcg gctctacgag aaatttattg ctgatttaaa
    18601 aattgccggg gttccgaccg aacagggtat cttcggagca atgatggcgg ttagtttagt
    18661 caatgacggt ccggtaactt tcgtgcttga tagtaaatta cggtgaaatt atgcgatcta
    18721 aggaacctgc cgtctataga cttccgataa cagcttggcc cgaaagcgat cgcccgcgtg
    18781 aaaagctgat gaaagccgga cctatgaaat tgacggaagc cgaactgctg gctattttat
    18841 tacgtagtgg agccggaggc aattcggctc tggatgtcgc tcgtgagatt ctgcgtaaaa
    18901 gcggcggact gaatgctctg gctcaaatgg attatcacga tattctgaat ctgaaaatta
    18961 gaggtatcgg cccaactaaa gccgtcacca ttgcggcagg cttgcaatta gcacgccgtc
    19021 tgcaggctgt cagcgtatct ccgccagaaa tgattatcca taaaagcgat gaggtggcaa
    19081 aaatttatat tccccgtctg caggatttga aaaaagagat ttttatggtt gtcttgttag
    19141 ctagtaacaa caagattatc cgtgatgcaa tcatctctga gggaatttta aatgcgtcag
    19201 ttgttactcc acgtgaggtt ttccgcgaag ccattctggg gatggcggca gccatcattt
    19261 taattcataa tcatcccagc gggaatccgg agccgagcaa tgaagacctc caattgactc
    19321 gccagatgaa ggaagttggt caagcgatga atattccggt tctcgatcac attatcatcg
    19381 ctggcgctca atacaccagt tttgcggata aagagttgct ctgataaaat gtttatatta
    19441 attttgaagc aggaaaaagc tattacaaat gaacactaat aatactcaat tccaaattac
    19501 gggaaaggtt caatctggta taatacctac tcacaggctt acagcaactt ggcaactttt
    19561 taataattac agattctcct tcctcaccat agacggaagt aattaaaaaa caaggaagag
    19621 agtgagaatc aaaagaggtt tgtgatggaa attatcgaac gagcgactct gcaacaacat
    19681 attaccaata cgccggatag ttatttgcgt cccctcttgg cggcgcttta tctggaagat
    19741 caggactgcc aagcggcgat cgaactctgc cgacaagacc ttgccattca tccgttctcc
    19801 acgattggac actacatttg ggcgctggcg gctttagcaa tgaatgatct gaaccaagcc
    19861 gctattcatc ttcgtgcaat tattcagatt gatgggaaat ttttacaagc ctactacaaa
    19921 ttggctgaac tggggggaga ttatcttaat caagcggaat taaaatattg ttatgaaaaa
    19981 atttgcgact tgaatcccta tgataaaaaa agtactgata aactatctgc ctttcctctc
    20041 gaactacaat ctcagaaacc gcaatcgcca gccaaaccgg agcgaactga tcaagtcgaa
    20101 gaatccagcg aaaaggcgat tatggctgag ccaatcgaat cagccccgga aattgatccg
    20161 ctcaccggcg ctaaccgtct ttctcaattt ttcgagtcaa taaaaaaacc tactgaatca
    20221 ctaaaatctt ctgggattca accaacaccc gtcccggaag aggaagccca tttcgggcca
    20281 atcgaaaaaa cggcgataga aaccgagccg acagtggaac cggaaccaac catcagcttc
    20341 gatgaaccct tcccggaaga acctcaggtt aaagaatcat ccggctcttc ctcgattagc
    20401 gacttattca gtcgtctgcg cgaaaagcca ttcgaggaag tacaaaaaga aaattggctg
    20461 aaggaacatt tgtctggtga aacgccgtcg cctgcaatgc cgactataac tgaatctgac
    20521 aaaccggctg tcgaacctca agccgtctct cagcctcaga gcgaaacaaa agcgacggac
    20581 attccggcta aaagtaaggc gactgcaacc cccaaagttg cgcccaccaa agcaggcaaa
    20641 gccagccaaa aaccgaaatc agcttctaat aagcaacgtc agtcgcctca accgaaaaaa
    20701 agcgccgcct cgcctggtaa tatttcattt ccaattccca cctggacctt ggttgatatt
    20761 ctaatgaaac aaaaactcta tgaacaggca cttaacattc tcgatataat cgaatccaag
    20821 tctaaaaatg aaaaagacct agaaaaagtc caagcaagcc gagcgaaaat cgtgcgatta
    20881 atggctgagg aacaatccgg cgaagcttga tttcagcaga ggaaatattt taatttctta
    20941 ggagcactta aacattggtg cactccataa gaactcatat attaaagatc atcgataatc
    21001 ttaaccttat cccattggta cttttttcgt aactcttcca tctctttctt ctcagttttt
    21061 atcctctcat aataactacc aaatttccaa tcttagtttg attggggcct tcataattta
    21121 ccatttaggg ctccaaattg atttgttaaa tgcataggtt ttgtcaaata tcaccctaaa
    21181 tatctgttat tacaatatat tggatacttt ttcaggaaac cctatttagg gttaacgaaa
    21241 aattttattt ttagtcagta ttaatcccaa tcaattctcc ttctttaaaa cgtgggctcg
    21301 ccgtttgtga cgacgagcac cactctatgt caactgcccc ttttcggtgt ctatttacaa
    21361 gctatttttt taaaagtcaa ggatattttt cgaaagtttt gtctgatcta gggacatggt
    21421 atctattata tagaactcat tcttgtcctc aaatataatc taccctccta atgaccaatg
    21481 accattctca gccttatttt tcttggtctt ggaagatgaa accgccaagt tcgccaagaa
    21541 ccgctaaatg agaaaaatcc caaatctcaa attccaaatc acaaatagcc aaaccaattt
    21601 ctgcgaaact gtctcctgtc tccttttctc agcctcagcc tctttactaa tgaccaatga
    21661 ccattctcag ccttattttc ctggtcttgg aaaaataaac cgccaagttc gccaagaacc
    21721 gctaaatgag aaaaatccca aatctcaaat tccaaatcac aaatagccaa accaatttct
    21781 gcgaaactgt ctcctgtctc cttttctcag cctcagcctc tttactaatt actaacgacc
    21841 aatgcccaat gaccaatgac caaccttcct gttctttttt cctttgccga aaaaagaacc
    21901 aaaaaaggca acggctaaag aacatctcac gggggttcgt tcagtcccag cgaatggaat
    21961 ttaaggcgca aaagtcatca agttcataaa accttagtga taaaggaaag ggtaatggat
    22021 aattacgaaa gtgctattac agcgctaaaa attccatttt ttccgctggg acttcgcttc
    22081 ccagccggag atgttcttga tgccgtaatg atatttagaa cgaataattt tagtcgtcct
    22141 gactgtttcc tgtctcacgt ctcctgtctc ctttctcctt ttctcagcct cagcctcttt
    22201 actaatgacc aatgaccaat gcccaatgac tattccttga ccggtttggt gataattgta
    22261 cgccattccg gaagttgccg ggcggctttt aaagcgtaag tacgatttct aaaaattcca
    22321 aacagagccg cgccgcttcc cgataaccca gcgtaaaggg cacctgtatt tagaatttgc
    22381 tccttcagtt ttgctaactg aggatatacc ggaaaaacga tttcttcgaa ttggttttcg
    22441 aattttaccc aatcaacccg cccaccactg ataagttcgg caaagttgta ggctggtttg
    22501 gcatttctgg gattaaactg ccggtacgcc cacgccgtcg aaatatttat ttccggcaga
    22561 aacagcaaaa tgacaaaatc ttccattaga attagcggtt cgagcacttc gccaattcct
    22621 cgagcgcctt gagtttttcc tttgatgtaa aacggcacat cactgccaat cgcacatcca
    22681 atacgagcca ggttgtcatc gctcagattg atttgccagg ctttattcaa aaatttcaag
    22741 acggttgccg cattggaact gcctccgccc agcccggcgc cgattgggat atttttttct
    22801 aaagtaatcc gccagggttg actatgtgga gcaatctgtt ccattcttcg gtatgctttg
    22861 gcacacaaat tgtccgtttt attagaaagt gctaaatgat ttgtggcgaa ttcaaaggtt
    22921 ttagccggtg tgaaggtaag gacatcgtgc aaatcaattt cctgaaaaat ggtactaagg
    22981 ttgtgatagc cgtccggacg acgcccggtg atgcgcaagg taagattaat tttggcgtta
    23041 gacttgattt taattgaggt ggctttcggc ataattcaaa gccgcttcga ggcgcttgat
    23101 acagatttca cgaccaatta tactggcaat ttggtgaatt tctgggcctt gcgtggtgcc
    23161 ggttagggcg actctaatgg tcatccagag cggtttccct ttaataccag ttgctttctg
    23221 aacattcctg acagccgcta taaagctgtc actgttgaaa tccggcttct tcccgtccgt
    23281 ctgatttatt ccaacagaat ctatcaaagg tatcagagtt ttcaggactg ttatggattc
    23341 cggtgtggtt aaataatcct gaatttcagg cgcctcataa gaaattgatg aggcaaaata
    23401 aaaagccgtt ttctgaggaa tttcggaaaa gttggacaac ccgtccttaa cagagttcaa
    23461 tgccgccttt aatcgagctt cgtcccaatg agcccgctca tccacgccag gctggtggag
    23521 acattcctct aaatatggtt tggcgtattc ccagagacgt tccaacgaca aattacggat
    23581 gtaaattcca ttcatccaat tgagcttttc gatgttgaaa atcgccccgc tatgactgac
    23641 gcgttccaag gaaaattcac gaattaattc gtcgagacta aaaatttcac ggtcgtcgcc
    23701 cgggttccat cctaataaag ccaggaaatt tatcagagct tccggcaggt agcctttgcg
    23761 ccggtaatcc tcaaccgcaa catcaccttg ccgcttcgaa agtttacttt tgtccggatt
    23821 tagcaataaa gaaagatgcg caaattgcgg caattcccaa ccgaaatagc gatacaataa
    23881 aacatgcttg ggtgtgctcg gcaaccattc ttcgccgcga atgacatggc tgatttgcat
    23941 taagtgatca tctacaacat ttgctagatg gtaagttgga taaccatctg atttaagcag
    24001 aacctggtca tcaatctgac taaccggaaa agtgactttg ccccggatga gatcgtcaac
    24061 ttcaatagca ccagcggctg gaattttcat ccggatgacg tactcttcgc cagaagatag
    24121 atgttgttgg atttcttcta ctgacaaatc gcggcaatga ttatcgtaac gcggtggtat
    24181 tttctgggca atttgccatt ctttcaattt ttttagtcgt tctgctgaac agaagcagta
    24241 ataagcatgc cccttttcca gcaatatttg agcataatca cgataaagct gaagtcgttc
    24301 cgactgcaca tacggcccaa agtcgccgcc gatttgcggt ccttcgtccg gcttgactcc
    24361 agcccattca agggttgcca gcagattttc gacggctccc gcgactttgc gacgttgatc
    24421 ggtatcttca atgcgcagaa taaaacgccc gttatacttc ttggcaaata agaagttata
    24481 gagtgcagta cgtaaaccgc caacgtgtaa aaagccagtt gggctgggtg cgaagcgagt
    24541 tctaatcact gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccgccaacg
    24661 tgtaaaaagc cagttgggct gggtgcgaag cgagttctaa tcactgactt ttttctccaa
    24721 ttttttgcct tcctcttaaa tcaccataat gatgtcaacg ggacgttcct aacattacaa
    24781 gagttaccgt cgccaaagcc gctatccctt cgccactgcc aatgaaaccc agtccttcgg
    24841 tcgtcttggc tttcacagaa atgtcaccga ccggtatttg taagcagtca gcaagactgt
    24901 ggcgaatttc cggaataaac ggcaatagcc gcggtgcctg agtgatgacg gtggcatcaa
    24961 tctggctgac gcgaaagccg acggagacga ccatttcacg ggttttttcc agaagaatca
    25021 aactggaaat gtctttatat tgcgcatcag tatcgggaaa atgagtgcca atatcgccta
    25081 gtgcagaagc tcccagtagg gcatcaatga tggcgtgagt caatacatct gcgtcggaat
    25141 gtcccagtaa gcccttatca aacgggatgg ttactccgcc caaaatcaaa gggcgaccgc
    25201 tacaaaagcg atgaacatct attccactgc caaaacgata tctcatttca aggctctctc
    25261 agtttcagta ttgattttgc aattttcaag tcattttgac aagtaatttt taaattgtca
    25321 ggcgtgcttt tgacgaccgc tacttttcca ccgattaatt ccactaacat ggcatcatcg
    25381 gtggctataa tattattttg ccgagcgttg gcgtaggcac gctcaagcac ttcccgacgg
    25441 aaggtttgcg gtgtttgagc ctgccagact cgactacgtt ctattgttct tacaatctga
    25501 ccatcctcaa cttccttcaa agtatccaca gccggaattg ccagaattgc cccgtcaaac
    25561 tctgagcaaa ggcgaactgt ttctcgaacg agttctggca ttacaaacgg acggactccg
    25621 tcgtgaataa ctacaatctc cgcttgtgaa tcaatgacct gcataccgtt aaagaccgaa
    25681 tcctggcgat gttcaccacc ggcaatgatt ttgatcggga cgctactcgg taatggcaaa
    25741 aaggctttta attctttgga attgatccga tcataactaa cgacaacaat tagttcacta
    25801 aggataccga tttttaacag gttatcaatg gtataagcta ggagcggctg accttttatt
    25861 tttaaaaatt gcttggggat ccctaaacca aagcgcttcc cttgcccggc ggctaccaca
    25921 attccactaa tgaaagcagt gttaggaaac aaattgttaa actaaataat aagcattgaa
    25981 tcgccatagc tgaagaagcg atatttcttc tcgatggctt cttgatatgc cctcataatc
    26041 agctcatggc caccgaaagc cgaaacctgc ataatcgtag tggatttagg ttgatgaaaa
    26101 ttggtaatca gagcatcaac catcttgaac tcgtacggtg gatgaataaa tttatcggtc
    26161 caaccacgcc gcggtgtaat ctggaaacct gaaactgtga ccgtttcaag cgtccgcacg
    26221 acggaagtac ctaccgctat gatcctttta cttttggagc gggcattatt tatagcgaga
    26281 gccgtttcag ctgaaacctc aaaatattcg gaatccatcc gatgacgaga caaatcctcg
    26341 acggtcaccg ggcggaaagt acctaaacca atgtgcagaa caatgggaaa gattttaacg
    26401 cccttgtctt taattttctg cattaattct tcagtaaaat gcaaaccggc ggttggagcg
    26461 gcaactgcgc ctcgtttctt ggcatagaca gtctggtagg tttccttatc atcttcagtc
    26521 ggctcgcggt caatataggg cggcagaggc gatagcccaa cctcatcaat caatttatag
    26581 agggtttcct tgggaatgtt attaaaacgc acgacccgac cgcctgaaac ggtattgtca
    26641 ataacatcgc actgaacacc tttagcaata gtcagtttat tgccaatccg gactttgcgg
    26701 gctggtttga ccataacttc ccaaagacta ttttccaatt cgcgcaaaag aaatacttca
    26761 actttagctt ctgtacggtc tttaaccgcc caaagccgag cgggatagac tttggtttca
    26821 ttgataacca gcaaatcacc gggattgaga taatcaataa tattgtaaaa tctcttgtga
    26881 gtgatagtct gttttttacg gtcaatgacc attaatcggg aatgatcgcg cttacttaca
    26941 ggatatttag caatcaattc ctccggcaca ttataatcaa aatcggaaag tcgcatttat
    27001 tttcctccta cgtaaacagc ttttgctgag agctacttat atttttatgg aaatatttat
    27061 aggttgctgg tgttgcctgg cgtccgcggg cggtccgctg aattaaccct tgctgaatca
    27121 ggtaaggttc ataaacatcc tcgatggttt cagcttcttc accgactgcg actgcaagat
    27181 tattcaaccc gaccggtccg ccggaaaatt tatcaatgat agttaaaaga atttggcggt
    27241 ccaaattatc cagtcctaat tcatcaattt ccagcatttt cagggattgt tcggcgacct
    27301 ttttatcaat tacgccagcg gctttgacct gggcataatc gcgagtgcgg cgtaaaatcc
    27361 gattagcgac gcgcggtgtt ccgcggctac gcttggcaat ttcggcggcg ccgtccgggc
    27421 gcagttcaat gtttaaaatt tgggcggtac gcatcagaat ctggaatagt tcgtccggat
    27481 tataataatc cattcgcaaa attactccaa agcggtctct caagggcgag gtcaaatgtc
    27541 ccagccgggt ggtggctcca attagcgtaa actgctcgag gttaagctgg atactgcgag
    27601 cattcggtcc tttatcaatg acaatatcaa tatggtaatc ttccatggca gaataaagat
    27661 attcctcaac cacgttgttc aagcgatgaa tttcatctat gaaaaataca tctcgcgctg
    27721 ccaaattagt tagcatacca gccagatcgc cagctcgttc tacaaccggt ccggaactgg
    27781 tgcgaatgtt tactcctagc tctttagcca ctatgtaggc aagagtagtt ttgcctaaac
    27841 cgggcggccc gaataaaagg acatgatcca aagcttcacc gcgttcctgc gccgctgaaa
    27901 tgtatagttt aagattttcc accacatttt tctgcccgac aaattcttca aatgccgaag
    27961 ggcgtaaagt tttttcatat tcgtaatctt cggccagctt ttggggatca atcattttag
    28021 aatgcatccg aaaatcctga ttttcgtttt attgatattt taatattgtc ggtttaaaac
    28081 aatattgcag ttgcaggtta attcgtgttg aaataagtaa aataaattcc agtagtgatt
    28141 taaaattctt tatattcttt agcaatacat taccacaaaa tttggcataa tttacttaaa
    28201 aataagccgt aaagcaatgc tgtattatag atttaccgac gaataattag ggatctggat
    28261 tgtttatact aaaataatct gacgacgttc tttgcccgta gagatgtagg aaattttaac
    28321 ctcaagtaga tcttccagag tctggaggta ttggcgagct tgaaaaggca actcgtctaa
    28381 actatgtgct tcagtcgtgg atttttgcca gccgggaagt gttagataaa ccggaatggc
    28441 attctttaaa tctaataatg gaaattcgcc atttgcgtaa tgagtacaaa tttttatctc
    28501 tgccagatca tccagcacat ccagtttagt gaatgccagg gatgaaaatc cattgatctt
    28561 gaccgcatac ttgccgaggg cggcatcaaa ccaaccgcac cggcggcgac ggcgagttgt
    28621 agcaccgaat tcatcaccac gcgcctgtaa atgatcgccg actgcgtcat tcagttcggt
    28681 gggaaaagga cctgcgccta cccgggtagt ataacatttg aaaactccaa ttatagaatc
    28741 tatccagtgt ggtccgatac ccaatccggt agcaacatta ccggcaatcg tattcgacgc
    28801 cgttacaaag gggtaagtgc caaagtcaat atctaacagt actccctgag ctccttcagc
    28861 caaaacgttt tttccgcttt ctaaatagga gtgaattcgg aaaatagtat tgtccacata
    28921 gggaagtatc tcgcgcaccg catccaaaaa tacttcaatg tcatctgcca atttatcctc
    28981 ttctgattcc gtcaaaactc cctgctttcg cataaaaatg gttcgggagg taatgttttc
    29041 tcttaggtca ttttcattaa ataaatcgca tactcgaata ccattgcggc tgattttatc
    29101 ggtataacag gggccgattc cgcgtttggt ggtgccaatc gctcgggcgc cgcttatatt
    29161 ttcataatag ttatccaaga ttttatgtag tggtgtaaca acctgcgcca gtggcgaaat
    29221 atgcagacga ttacggacgt caatcccttt tcgctggacg acatctattt cattcttgag
    29281 accaaacgga tcgaaagcca ttccgttgcc aaggaggcaa tgcgtttgcg atcgcaatat
    29341 cccgctggga atttgatgca gaatggtttc ttctgtgcca atctgaactg tatgaccggc
    29401 gttagcgccg ccctggaagc gcgctaccaa atcggctgac tcgctcagaa tgtcaacgat
    29461 tttacctttg ccttcatcgc cccattgggc accaatcacg gcagttaacg gcattttatt
    29521 ctcctaaaaa ataaaatgct cccgagaaag ggagcatttt tcattgattg ttttatatga
    29581 atcatttagt ggagcgtttg aacctacgcg accagaaaac cactaacgga cctgcgatat
    29641 aaatcgtcga atatgtcccg gcaatactgc caattgtcat ggcgatcgca aagatgcgca
    29701 cttcacctgg gactaaaagt aaacataaga ccacgaataa agtcgtcata tcggtaataa
    29761 tcgttcgact gagggtctcg ttgatactga tgtccatgat tctctcatag gtttcgttac
    29821 gatgtaattt caagttttcg cgcacgcggt caaatacgac gattgtatca tttagtgagt
    29881 agccgatgat cgtcagaaaa gccgcgattg ttgataggga tacttccagc tgaaaaatag
    29941 taaaaaccgt tagggtaatt aaaacgtcgt ggagcaacga gagaatcaca ccgatagaat
    30001 attgaaattt aaatcggata gcaatgtaaa tacccataac gataagagag aaaaagatag
    30061 ccagtagagc cgctccctgc agttctttcc cgatctttgg acctacttgt tcctggcgta
    30121 agatgttctg gtcgctaatt ccaagagtag atttgataac atctaccaga ttagcactgg
    30181 ataaatcctg cggcactcgg atgatatatt cattttcgcc agatttctgg ataatactgt
    30241 tttcgaggtt gatagttgcc aggtcattgc gaatgtcctc gattgttcga ggttgttcaa
    30301 atttgacctg gattagagtg ccgcccgtaa aatcaatccc aaagttcaat ccgcgaaaaa
    30361 ttaacgatat aaggccgata gcgattatta ccagcgaaat cgttatagcg gtcttgctat
    30421 atttaataaa aggaatatca gttttcttaa taattcttct catatcaatt tatcctgatc
    30481 ttaaatgcta agagttttaa tagcatgttt ttcgattcgc cagttaaaga aggtgcgggt
    30541 cacgaatata gccgtgaaca tactggatgc gatgccccag aaaagcgtta cggcaaatcc
    30601 tttgacaggg cctgttccgg tctgcatcaa tattatagcc gttagcagag ttgtaatatt
    30661 tgaatccaga atggcggaca atgcccggga gtaaccggta ctgaccgccg cacttacagt
    30721 tttcccacgt tccagttcct cgcgaatacg ttcaaaaatc aatacattgg cgtccactgc
    30781 cataccaatt gtcaacacca gaccagcgat accgggcaga gttagagtcg ctcgtagcat
    30841 tgccataaca gccagtgtga aaaccatgtt caggatcaaa gccatatccg ccaatagtcc
    30901 agaacgccga taataaaaaa acataaacag cattataacc aacagagcaa aaataccaac
    30961 ccggataccc acggcgatag aatctttacc gagcgacggt ccgacggttc tctcctcgac
    31021 aatagtcata ggggctggta atgctccagc gcgaagcaca atagcgatat ctttagcctc
    31081 ctccatatcg gacatacctt cgatataagc cgacccgtcc ggtatcttaa ctcttatata
    31141 aggcgcagta taaactttat catcgaggac taccgccagc tgtttgccga tattcgcgcc
    31201 ggtaactttt gcaaatatgc gggcgccctc gctattcata cttatattca cgatcggctg
    31261 gccactgctt tgcgaaccag caccgcccag cgaagcagaa gctttggtta tgtatttgcc
    31321 tagtaaagcc ggttcgctat ggacgtggta taaaatataa aaattttcag tacgaccatc
    31381 ctgaagagta cgacgtacag gtttggctga ccagagaaat ttagaatcaa aaggaatcaa
    31441 ctttttcaca ttggggtctt caagaatttt cttgacagca taaacattgc gttccggaac
    31501 tccaatctgg ttatcaatat ttctaagtaa agaagaaaac gggcgttctt tgtaaatttc
    31561 ctcatcaacg atcaagccgg tatcagcctg ttcggcggta agtccggttt cggtagaagt
    31621 ggttcccagt agttcatcaa cgctgatcgc cttgtcctta gattctttca atgctacgat
    31681 tgaatcggct tcttcgtgag tttcaggcga gacctcgtct gccttgcccg tttttaaata
    31741 attatctaca gccgttatga attgctgggt aactgccgca tcttttacca gaataaattc
    31801 cagtaaagcc gtactttgaa tcaattctct agcccgacca atatcttgaa taccagctag
    31861 ttccacaata atccggtatt tgccagattt ctggatcgaa ggttcagata cgccaaattg
    31921 atcaacgcga ttacgaatca cttccatagc gcggtcaacg gcatctttgg cttcttcttc
    31981 cagactcttc agaatctcgt cgttcttgaa tccgcgtcca ttaaaatgac gcactaactt
    32041 taggtccgat tcattgattt ttcgacgaaa tacatcgaaa aaatcctctt cgttagcgat
    32101 ggcttcgtca ttagcttcct ttagaagatc cataaactgg ggcgtcttat tggatgccag
    32161 agattcaatc aactttggca gatcaacttc catcacaata tgcataccgc cctgcaaatc
    32221 aaggcctagc cgaatgaccc ggcgttcgta tttatccagc gaaccatccg cccgcatcgc
    32281 ttcccgtttc tcgtcagaaa aggaatgata agcgactgtc caatataatg aatagcacgc
    32341 tagaacaact actacaccga taatgataaa tcggacaata ttctttttaa acattcagaa
    32401 tcacctcaaa gtatatctta tatttagttt aaaaaaagca tgtgaattta acttcaagag
    32461 tcgtttaatg tcaaacaaaa agccagcctg gtcaaataaa tccaaatttc aatcctgaat
    32521 ataaataaag gctcgtgtca actcgtcaga gccataggtt acgacaatcg gatatttttt
    32581 tcctaaacca tctactgaat atttcttcca ttgcaattta cgggtatcta ccagacaatt
    32641 gcgatcttct ccatcgttat aactacccgg cggcacaaag ccctttagat taacctgcaa
    32701 atcggcactg tctaatccgg gaattaaaac ttcaatgatt ttaattaaat ctcctcgttt
    32761 gacagttagc gtttgaccat cggttagctt acgctgaatt ccattaactt caattatata
    32821 ggcaatgctg tcaaacgtcg aaccgattgt ctcgacttta acactgccaa ttataatatt
    32881 atcacgtctg acgatgatgg aggtcggctt taaaacggga atcggttttt ggaaatcttg
    32941 agcgttcccg atgccgagca catcgcaggt aagaccacga ttataattcg attcgatatg
    33001 cttaacacgt ataatatcac cataattgac ttgaataaaa ccatcgtttt cgacgatttt
    33061 cgtctcagta ttattgatag tgataaggag gtaaatcagt ttaggtggct caaataggac
    33121 ggctgggtgt tccggttcaa ctcccatcag cttaaaaaat tcattaattg cgtaattatg
    33181 ataacgtatt tttagttcga cattttcgag atttttcgat gattcaatgc cgaaggcagg
    33241 aatgtgaaaa gttgttagcg cataataggt cgccgacttc ctctgctccg ggtatttagt
    33301 atcaggactt aaggtattag tattgaaaaa actaaatttg tgttcgggat tctccactcg
    33361 taagttgata ccttttatta ctttttgagc catttcctct aatttcaggg tatcaccatt
    33421 gagtaccaca tggtcggcat cggcgataat tgactgtcca tagcagtacg gattgcggtt
    33481 cttgtcaata taagttttac tgtaaaaacc actaccatcg tgcaaattga gaaaaagatc
    33541 gctttcggac attaactgct taataattgc tactatttga tcttcaatat cagtggattg
    33601 tttacggtcg aagcgccggt tcatatcacc attaacgccc cgcttattaa gaattattga
    33661 atggaagttg gcgcgcggga tgacaatcag attaccccta tcaagcacta gatcagggta
    33721 aaggtcagct gataagtagc caccaggttc atcgccctga atacccccca ggataaaaat
    33781 agtgttgcca tcgaagcgtc cgtaaagttt atagacatgc agttcgttgg gcgtattctc
    33841 aaaatatacc tcgtgttccc gcgaaggacg cttgatttcg gcagttaagc cagacgcgac
    33901 ttgccaggaa ataatcagtc caattaacag aatcgttttt cgggttttat ttttcttcaa
    33961 cccactcttc cccaataatc ttgatatttc ccggaaaacc tttaagtgtt agcgtcttta
    34021 agccaatatc ccgataacca tctccctgat aaatctgtct gaaagttact ttccagactt
    34081 gctcttgagg cgattgaatt ctcaaagcac tccatttcac actgatattt tgcactgatt
    34141 ggaaaacctg gtctttgtaa tttttccatc cggatagatc atactcgccg ctggtgaact
    34201 cttccccata gaaattaata taagtatcaa tatccttttg ctcccaagca ctcttccagc
    34261 cggataaaaa ttgggtaacc atttcttcgg ttgtcaggct tggactggtc ggacgaaaag
    34321 tttcccgcac aattctccaa ttcgaatcaa tcggcactaa aattaactgc ttctggcctt
    34381 cactataaaa tgttgggcaa agatagcgct ggcgaaattt caaatgcgct tcacaatcag
    34441 agactagtac actgatttcg tcaaccatta cagttttcca ttggtatctt ttttccaggt
    34501 cagttttata ggacttaaat tgtttcagat tacgaccatt ttcatcaagg aagttagggg
    34561 cataatagtc acaatattcc gccactgcgc cgctgtccca atttttcgtc caaccattga
    34621 tgtactctgt ccagaaactt acagcggctt ttaaattctc cggagctaca taataatcgc
    34681 ttaaattatc caaaatgatt accggggtct gacggactac gatgtatttt cggagatcta
    34741 atatatgact attttcaaga gaaatacatc cttcggtaat aaaatctttg atctcttcgt
    34801 cgcgtccatg tatccaaata ttcgatccat tacgtttctg taaacgatca acataattcg
    34861 gataatccag cgcataagcc agcggcccat atctagcggt taagcgattg tccggtactc
    34921 gaccaatgat ataataaata ccttccggtg tctttagatc gcctgaacgc tccttatcac
    34981 ccctccgcct tccggttgta attcggtact cttttacaat ttccagagcg ccgggcgcgt
    35041 tacttcttac aacgaacatt ttttggtgtg acttatcaac gagtaagaca ttaccggttg
    35101 agtcggctga aatattgagc acgttcgaga atagattttc agccagactt acggcattta
    35161 aaacagctat taatatcaag atcgaaattt ttcgcgccat atttgtattt ctgaatttta
    35221 agtttacatc actgattttc ataattaaag tgcgccaaaa ttagataata aaatcgtttt
    35281 ttaaaatagg caattttcgt tggtaagata agttgaggct gagactaagg tcgaggttaa
    35341 gattaaaact gaggcaaaat catagcaacg aggcttaact taataccact tcaccaataa
    35401 ccaataacca atgaccaacc ttcctgttct tttttcctat gccgaaaaaa gaaccaaaaa
    35461 aggcaacggc taaagaacat ctcacggggg ttcgttcagt cccagcgaat ggaatttaag
    35521 gcgcaaaagt tatcaagttc atataatgtt agtgatatag gaaagggtga tggatattta
    35581 cgaaagtgct attacagcgc tgaaaattcc attttttccg ctgggacttc gcttcccagc
    35641 ccgagatgtt cttaatgccg taatagtaaa ttgaacgatc aggtggggcg ctcgtcatta
    35701 ctcaacctaa aagaaaggct aagactaaga aaagaggtta aggctgagaa aaaagactaa
    35761 gatttgaatt ctctctcaac ctcaatctct tttctcaacc tctttcctca gtctcagtct
    35821 ctttactcag cctctttacc aatgaccaat gaccaattgc caatgacaac catctcaagt
    35881 caaggacacc ccataaaaag gcaaaacctg tggagcaagg taatggcttc cggctatcaa
    35941 taatatttca tcagatttta tctgaatttc taaaaaattc aacagccgtt caagtttatt
    36001 atctacataa atcagcctgg atggaatttg tttacgaatt acgtcggcta atatttcggc
    36061 tgaaactgaa ggatggtcag gaatttccgt taaataaata tctccaccga gtggctggat
    36121 aattttaccg atgcgatcgt atttctttac gctacccagt gccagtacta aatggaccgg
    36181 ttgagttgga aatatttgct ttagcgcctt tactacctgg cgaataccat gctgattatg
    36241 gccaacgtcg aaatagatta atggcttgtg ggacagtata tgcaagcgtc cgggccaaat
    36301 caccccagtg attccctggc gcagaacttc cgctgaaagt tccaagtctt tcactcgcat
    36361 cagggcactg acagccgcct gtaaatttat tagctgatga tcaccaatta gcggaaactt
    36421 aaccgtgtca attttaagat tatttacaat tagacttaat acctgtcgtt cgggaaaacg
    36481 ttggatattc gtgatttgac attgttcagg agcatagtaa aaatcgccgc cactggcatt
    36541 gatttcttta cttaaaaagt tgaggacgcg tggtgtttga cgtgcgacta tacaaggaat
    36601 cccgcttttg actattccgg ctttttcata ggcaatttgg gctagtcggc gaccaagaaa
    36661 ttcctgatga tcacgcccaa tgggagtaat gacggtcact tccgggagcg tgacattggt
    36721 ggcatcaaaa cgtccgccca taccggtttc aagaacggca taatcaactt tgtttttttt
    36781 aaaaaagtcc aacgccatag ccgttgtggc ttcgaagaaa ctgcagttta gttcgtcaat
    36841 tagcttgcgc caagattcgg tgaatagagc aatctcggca tcggggattt tctggccatc
    36901 aattcgaact cgttcgttga aattcaaaag gtgtggcgaa gtatataatc ccactcgata
    36961 tccagcggcc tgcaaaacat tagccatgat tgcacaagtc gaaccctttc cgttagtccc
    37021 ggcaacgtgt atcaccttat aagcgttctg ggggttgccg atcgcatcga gtaatttttg
    37081 gatacgctct aagcctggtt tgataccgcg accattcaaa gaaaataagt agtttaaaat
    37141 aggccgcaga tcagcggtat ctgaggacga cattagcaac aatctgcgag gttgattcgt
    37201 tcgatattag cgattggctc acccagaaat cgcatcgcga tttcttcgaa cttctgcggt
    37261 aaatcgctca cataaaaacg atgttccggt gttctttcca aatcgtttaa tagcccctgc
    37321 tgattcagaa tcaagcgtac ttgattagcc gtttctatag cggtatctat aatctggata
    37381 ttgctatcaa ctacccgctg aattacgttt ttaataattg ggtaatgggt acagcccaaa
    37441 ataagagtgt caatttttag aacgagattc tgcaaatatt cccgtgcaac tatttccgtt
    37501 acaggtgatt caccccatcc ctcttcgaca agcggaacaa acagaggaca agcctgggag
    37561 aaaacctgca cagatttatc gagagcggtt aacacttcaa cgtattttcc ggaattaatg
    37621 gttccggtag taccgatgac gccaatcttt ttggcgggcg atattgccag ggctgaccgc
    37681 gctcctggtt cgattacccc aataatcggc aagtcgaatt gatcttgcag taattccagc
    37741 gcgacactag aggctgtatt acaagctact acgatcattt tcacgcttcg ttcggctaag
    37801 aatctggcga tttggatcgc aaatttttgg acggtttcga cgcttttatt accgtagggc
    37861 actcgggcag tgtcgccgaa gtaatatatt tgttcctgcg gtaattggcg aaaaagttgt
    37921 ttgactacgg tcaacccgcc cagacccgaa tcgaaaaccc caatgggcag attccgcttg
    37981 ttacttactt tgagccactg atttgtcaca atattccttg aattcaataa cgcttttata
    38041 aagggctaaa gcgattttct ggcgatgagc cggatcgttc aattttttgg catcgttgcg
    38101 attatcatta taaccaagtt cacatagtaa attgggcatt gaagcgccga ccaggacgta
    38161 aaaattggct tgcttgacgc cgcggttttt ctggggcaca tattcagaaa tattgcgcga
    38221 taatatttca gcaaatttct cactatcgtg caggtaggta ttataaacca tattagccag
    38281 gatatgagaa ggattatcat agtccttata cttagcctta tcttcgctag tttctaattt
    38341 aatgacttca ttttccaggc gggcaacttc tatagattcc tgcgtcctaa tagtcggatt
    38401 ttggaggtat aattcgattc cctcagcatt gccttttgta ccagatggaa gcgcattgat
    38461 gtgtaaactg ataaaaagct taccggcgga atcattagcc aggcgggttc ttttccagag
    38521 aggaataaat tcatcgtttt ttctggtata gatcactttt agatcgtcgc gttgctccag
    38581 caatttaccc aatcgaagag cgatgtccag cacgatatcc ttttcgtgtt tatagcccca
    38641 acgaccgggt gtgccagaat ctttccctcc gtgtccggca tcaatgacaa tggtatcaac
    38701 aatccaggta tttttgactt cttcaatttt ttttagtgtg gcactggtca gtggcacatt
    38761 taaagtaact aaaattttac cactgcgggc atcgtaatag gcatttgccc tgtcatattt
    38821 ttgcttcaat ttaagggtaa tttgaaccga attccctact ggaatagctt caacgattgc
    38881 cactgaagga atcggatccc gacgtgaaag cgcggctgaa tcgcaggtag ctccgagaat
    38941 agtaaggtag aaccactcat caccgcggaa gaaagaagaa aagtctgaac tttcgtaggg
    39001 agcattggtt aaaattttca tcgtcaagcc gttgccgcgt tcttcaaact ggatgtcgta
    39061 aatgataccc ggctttgtcc caaaagttgt taccgtagat gctgacggag cttgagttgc
    39121 tcccggcaat tctactagct cataaattga agagcttctg tcaagatgat attgaaattt
    39181 agggatgaca taccgcttca tcaggtcaag aaatgagtag agtggaacaa aaatctcgtc
    39241 acggtttacc aaaatagctg gttgagacat ctgcagtatt ctagtatcaa gcataacgaa
    39301 agcactatga gccgtgattt tgaccgattt accggaaaaa tagagaacta tcttgccagt
    39361 ttcaggattg ttataagttc ttaatccgag cgcctgagca aagtctttcg ttgaaaaata
    39421 agcgcaatca ttaaaataga taatagagat ttccgtacca gcttgcgggt gaggagattg
    39481 aatgacaagc tgatccgcta ataggttatg acaattaacc aaaaataata gaaaatatat
    39541 ataaggtcta aatattttca caattgtcgt gaaattacac acttctatgt cataagtcaa
    39601 aggtcaattg caaatcacct gaaaaatcat acctgttgtc atttgtgtga atagacacat
    39661 tgctccgtcc gtagactgcg ccggctattt cacaattcga accgattcgc cgactgatta
    39721 tcagaaaata gcgctgacca gtgccggtta atagagtcat attaaagcgg aaaggcaggc
    39781 caggttcata aaaataaata tttaggtcgg acgaaccggt aaaatagtgg gtgttcccgg
    39841 cagtcacacg ggtatattta gaaatcgaga cttttcccac aatgctaagt gcctggctac
    39901 cttcagtgaa aggaattgaa tcgttccatc tgaaagttgt tctgatttca ccggcaaatg
    39961 acgagctaaa ggaatgcctg agatgcaatt taagctgata atcgcgcggt tgttgaggca
    40021 gactagtgtg atagtagcga ttgactttat accaagcttc aattaagtcg ttattgcgag
    40081 cttgacggct aaatccgcag agggattccc gcccattccg gattgctaca ccaggctcgg
    40141 aattttttaa ttcacgaaag aaatcaacat agccagtgaa ccgccagcga gccggtagtt
    40201 tgattttcag agcggaatat aaaccggttt cattgccgga aagactggag aacttgcgga
    40261 aaggattgcc gagcggagcg aaaaagtccg gttcgatata gcgccagccc gcacaaaaac
    40321 ttacacgatc tctacttaga tgaagatttt gaattattcc ttttttaccg gtttcctgga
    40381 ctgcccattc accgcttatc gtccaatcgc ttatttcgta attatgtaaa aatgatagcc
    40441 cgctaacttt gcgatgtccg tttaaggcaa ataaatcagt cgaataatta gcattataga
    40501 ccaaaattcc agccgagcct tgatcccact cgcccagcaa acttgctcct cctaatgttt
    40561 ccagcaaggc gtctttagct cggagttgac ttgagctaat atgcaagcca tcggagcgca
    40621 aatttttgac cgttccatta gtatcaatag tagcatcgag ccggtgatgt gaaatgaagg
    40681 ggattaactt gaaatgtcca gaggtgtact gagcggccga accgaaaaga tagcggtttt
    40741 cgtcagaccc cagataattg cttacccctg atcgcgaagt cttcagatta ccggttgcgt
    40801 cagtactgcg accactcatt agattattag aaaaaagcaa accttggccc cattgtaagc
    40861 gataggcgcc gcccaggatt tctaaagagt taaaatattt cggagaacgc aggctgatgt
    40921 tccaataatc accgagaata ggttcgtcgc tatcgttctc cacaattatt cctgtgaacc
    40981 aaccttgagg cgaataacag cgcgctcgtt ggagtgattt ctgtaaacta gtatattcct
    41041 gtttttttaa ggaaatgaaa tttactatct ggctcggctt cgttcttttc actgaaaaat
    41101 caatgaaata gctgatcgct tcgatttcgg cagttgtaaa aattgtatct tttaccagtg
    41161 ctttcagatt gaggctcttg gggtagtgac gttttagctc aattaattga gat
//
LOCUS       DAOSMP010000016        39810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_1049_length_39810_cov_15.536360, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000016 DAOSMP010000000
VERSION     DAOSMP010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 39810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39810
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1049_length_39810_cov_15.536360"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PKX09_04345"
     CDS             <1..335
                     /locus_tag="PKX09_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOU17084.1"
                     /translation="LGHTFTIFAGFKGGKGVAVGAGMIIVLYPLALLPCLLVFSIVLF
                     STGIVSVSSISAALSLPIVLLFFNYFGTSPIDKALLIFSIFIPIFIVYTHRANIRRLF
                     KGEEKPFE"
     gene            394..1608
                     /locus_tag="PKX09_04350"
     CDS             394..1608
                     /locus_tag="PKX09_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOU17085.1"
                     /translation="MSTYRFRLAIPLLGLLFLSGWAQIPCADFEIVESIPIETILDNP
                     EIRNTSEVWAEMIGAADETIDLEQYYISNAVDEPLEPIIKLIEQAARRGVKVRIIVDA
                     RMAKTYPETINRLTKTKNITIRQSQTFNRNDGVQHAKYFIIDGKEVFIGSQNFDWRAL
                     EHIHEIGLRICHPEYARLMTELFEMDWDYAVLNKMPNRKLTEPHRFKVAIAPGDTVKF
                     TASGSPARNLPDGMTSDEKVILELIRCAKKEINIQLLSYSPLDKKNSYWAKLDNALRE
                     AAARGVTVNLLVSDWNISANELPYLQSLAILPNINLKISSIPEYSGGYIPFARVEHCK
                     YMVVDESLSWLGTSNWARNYFYDSRNLGLKIENATFAKTLRNIFLKSWDSPYAWQIIP
                     GQDYPEKFHGEK"
     gene            complement(1769..4018)
                     /locus_tag="PKX09_04355"
     CDS             complement(1769..4018)
                     /locus_tag="PKX09_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOU17086.1"
                     /translation="MKKWSKILLVIGFLSAESLFAQVIWEKNQYKVHLQNNVLSLFYS
                     QDKIVEISSFSFNFIPVDTIIVEEVFEDSLILKLRLAEKDGFHADFPSEINLKITQSE
                     NTFHFFTSHQTFNHITVCLKDQNEHYFGLIEKLYPGNLKNPDLRGNVVDVDVYGLGNL
                     DYAENYASAYSAFFISSQGYGSFFDTFAKGKYKLGIGGITEIYHQTGTLDWYIFYGPT
                     GEKIHSEYYRLIGKPKYIPIWACGPIFWRDQNNGGKDELLTDIQKFNELQIPLTACFI
                     DRPYSNGAHEWSKMDFSDKFAEPEKWIKKINNDYRMQVMTWIGPLTFSDSDFPGLFPD
                     SRNYIDLTNPMALAEFERRLASNQYAVGIKGHKMDRADEHFPLTADWYEPVTETEARN
                     KYVYLYAKTIDKFLQNAHGRDNFNFARAAFHRSQPYLSALWGGDSRNNWYGMAGNMAN
                     AIRCGFMGFPVWGNDTGGYLGEGKIDELLYMRWLEWGVWNGMFEIKIDGAGGSGEDRP
                     PWKYSEQLQQVFRNVCQFRMEMLPYIYSCANTSYKNGVLMKPLAYMYPDDENTYTVWD
                     EFIFGSAFLIAPVFSADNERQIYLPQGNWIDYYEPNKEYSGPLTISQSIPLERIPVFI
                     RQNSIYVSGQIYQGNSKTWAGNLNGKENLIIHLYPGEINENTQFTYVDYLDGDTEKTM
                     TLQHQEGKILFSSPAIPIPSVVRVTCSEKPQKVFYGGKTATFNYDKQSKIAEIACPQN
                     RSIQLEIRY"
     gene            complement(4038..5483)
                     /locus_tag="PKX09_04360"
     CDS             complement(4038..5483)
                     /locus_tag="PKX09_04360"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HOU17087.1"
                     /translation="MRSNLTVLAVDLGAASGRVMAVNYDGNNFELKELHRFLNVPIIN
                     DGTLYWDISRLWQETKAGIEKGKALKPISIGVDAWGVDFALLDKQGVLLDNPVHYRDR
                     RTDGMQEKVIARISKQEIYSQTGIQILPINTLFQLSYLVEKQSPVLLNAETFLTIPDL
                     FNYWLVGVKACEFTNATTTQLYNPLQKNWAESLINKLQIPYHIFPDVISPGIQLGKYD
                     GIDVMTVAAHDTASAVAAVPTEIKNFTYISSGTWSLVGQEIATPLINESALKANITNE
                     GGVFGTFLFLKNVMGLWLIQECQRQWRKEGENYTSSDLVKLAKQEPPLRSLIIPDDPQ
                     FLHQGNFPQMIANFCQITSQPMPTTPGAIVRCVLDSLALDYKNVLEKLVGISNRPVEL
                     IHIVGGGSQNDLLNQLTANATGLSVFAGPAEATVLGNAIVQLITLGEIKNLSEARQIV
                     AKMRGIKRYEPKDSALWDGAYQRFLTLQKQF"
     gene            complement(5513..6718)
                     /gene="rhaI"
                     /locus_tag="PKX09_04365"
     CDS             complement(5513..6718)
                     /gene="rhaI"
                     /locus_tag="PKX09_04365"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOU17088.1"
                     /translation="MNPSGKSSNIFDNPHIDFEKVIHALKSQRIETPSWGYGNSGTRF
                     NTFACAGAARNVYEKIADAAYVHKLTGIAPAVALHIPWDKTDDYTALKQYAESKGIKV
                     GAINPNLFQDKQYKLGSLCNPSKAVRQKSIDHILACCEIMEKTGSNVLSLWLADGTNY
                     AGQDDLFRRKANLESGLKEIYSNLPQNCRMLIEYKFFEPSFYHTDLADWGMAYVMAMK
                     CGPQADVLVDTGHHALGTNIPHIVAFLLSEGKLGGFHFNARKYADDDLIVGTVNPFEI
                     FDVFTKLVSAGSKADKIVYMIDQSHTIETKIEAMIMSIINCQTALAKALIVDYPALQE
                     AQMNGDILVAQSILTKAFETDVYPLLEEVREEMGVPRDPLLAYRKDNYRNKIISERGI
                     SEANNGYPV"
     gene            complement(6779..6955)
                     /locus_tag="PKX09_04370"
     CDS             complement(6779..6955)
                     /locus_tag="PKX09_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17089.1"
                     /translation="MKIILVIVSLIGLVLTLLPSFLVFANAISWDTYLLLMNVGTIMW
                     FVSAPFWMEKKDEA"
     gene            complement(6955..8364)
                     /locus_tag="PKX09_04375"
     CDS             complement(6955..8364)
                     /locus_tag="PKX09_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010853201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17090.1"
                     /translation="MKSKRGNVLVKKLDEINEFERTPVPPSKLKGAGSFWGMYAGEHT
                     AGTEFVIGPLFVAHGVSAFDLVTGLLIGNLLAVLSWTFITGPIATRARITLYYFLEKI
                     AGKKVTLLYNLVNALMFCGLAGAMIAVSATAIGIPFNLPMPSLNDFFPNSIGWIVAVI
                     VVGAVTTAVAMFGYSVVAKFGKFAAPWMPLVFIASAVAVLPEIGVSSFSNFWEVAKER
                     IWNGVPLEGQSKFTIWHIIFFSWFCNLAMHIGMADLSILRYAKKWYYGVTSSAGMFVG
                     HYIAWLASGILYSLFLLESNNSLAFSPGQVAYRAAGIAGIACVIIAGWTTANPTIYRA
                     GLALQAVMPKSKTWKVTMIIGAITTFAAFFPALVMRLLDFVSLYGLILMPMGAVIFTD
                     FWLFPKLKLTQNYAEVRKIQFSWPVFLTWCITLLIAFLLPMEIYFKALPGWFIAIILY
                     TVLSYFQQKVLVAKKEKEK"
     gene            complement(8421..10112)
                     /locus_tag="PKX09_04380"
     CDS             complement(8421..10112)
                     /locus_tag="PKX09_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOU17091.1"
                     /translation="MKKKLSKKIACIAIFLNLVTQLLYSQENVIRIACVGNSITEGNA
                     MATKTLDAYPVALGRYLGAGYEVKNCGVSGTTLLKKGDYPIWNEKLFAEALNFNPNIV
                     TIMLGTNDSKPYNWIYKGEFISDYLAMIDTFRQLSSRPEIYICLPLPAFSGAWDIRDS
                     IIVNDIIPMISQVADSANVNLIDLHTPFLDKSSLMPDGIHPLIEGSDLIARILMEELT
                     GRRIESQTENDVALCKLAIQSGTNVPELTDGNSNTYAVFSSTSTPVTINLGDEVKVDL
                     VQINFTNISISEFAFTFAASIDSLDWTTLIDTTLLSASLLKCDSTALFSKTFQPIPAR
                     FLKFQIKENGIPDDSGLLINQINIFERRVVHAPIWGWELVSTSDKYMRVKVRTQKTSN
                     IGEYIKIYKQSKADGQFELYANYGSDKPLEMTSFISYGSLNRYYSVVFYNDVEIASDT
                     MTIIARSASDIKNNPKQVLLTNYNLFQNFPNPFNSATNIGFYLAELGEVNIEIIDLNG
                     RLIRSLSPRIFSSGINQIRWDGCNQNGIPSASGIYFYRLKIGDKPSLLKKMVLLR"
     gene            complement(10130..10966)
                     /locus_tag="PKX09_04385"
     CDS             complement(10130..10966)
                     /locus_tag="PKX09_04385"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOU17092.1"
                     /translation="MKCLKFYDIKIGFIILFGIVSIFRENGMAQKNEKNWWNNKKCAV
                     CLTYDDALNVHLDSVIPVLDSLGLKGTFYLSGNFQTVQERANDWKLAADNGHELGNHT
                     LFHPCDGKMPGRNWVNPDYDLSTYTLSRITDEIKMANVLLQIIDGKSKRTFAYPCGDT
                     QVGGVSYINVIKENFVGARGTETRLETIDDIDLYNIGCFMINGESGVELIDLAKTALK
                     NKALIVFLFHGVGGEHNINVSLEAHNQLVNFLKQNEKDIWIAPLAEIAKYIVNYRGKI
                     GK"
     gene            complement(11004..12308)
                     /locus_tag="PKX09_04390"
     CDS             complement(11004..12308)
                     /locus_tag="PKX09_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOU17093.1"
                     /translation="MLKFKRICLIMVLVLMNIVYAGEINPGYEVGVWQGFRSAAVTFT
                     FDDGCANQFTVAIPLFDQHGFKLTLYPVINWSGGIWSKLQSAAANGHEVGSHTVTHPD
                     LSGLSEAEQITELQNSQNEINSRISGQRCITIAYPYCNAGKTSICKQYYIAARGCSGQ
                     IEPKTPSNFMNISSIVCGTEGSVKTANDFFNKAKSAANSKGWVVFLIHGIDNDDGWSP
                     VTTENLTGAVDSLSAYPDKFWVSSFANVARYIQERNNVSITESVVTDSSITFQVADTL
                     DNEIYNVPLTIRRVLPEGWSAAEIFQNGEKINSQIVEIGTARYLMFDIIPDEGDIILV
                     KSVDNAIRLKNVPTIPYLKQNYPNPFNPKTTIAFSIPQKSFVTLKIYNLFGQEVTTLV
                     NESKTPGEYTVEFDGSYLPSGTYFYKIQAGDFSSTKKLILLK"
     gene            complement(12431..14593)
                     /locus_tag="PKX09_04395"
     CDS             complement(12431..14593)
                     /locus_tag="PKX09_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65"
                     /protein_id="HOU17094.1"
                     /translation="MGKNKQKYDKKRNLNELLAIVVFLSSFVVFFSCENNAKIDREAL
                     VKRHSPTLNASDPLNPFTVGNGEFAFSTDFTGLQSFPECYEETIPLVTQAQWGWHSFP
                     NLNQYSLDQVYEEYEVNGRKVAYASHQDSEAGEYLRSNPHRLHLGQIGFKFKNQDGLP
                     IRINEIRNIKQTENIWEGIIYSSFEVLNTMMEVETACHPVYDQIAVRIKSALLNQGKV
                     AISFNFPYASSRWGKNPADWDSPEKHFSTIVLESPNSVLIKRTLDTTEYCVKIYWRGD
                     AEFTKTDRHSFNLAIKSGKNFEFTCLFFEREDDQESASVTRTFSAARSFWQNFWETGG
                     AIDLSASKDSRAKELERRIILSRYLTRIQCVGSLPPQETGLTCNSWYGKFHLEMHWWH
                     AVHFVLWQKPELFEKSLPFYQRILPMAIRTAERQGYAGARWPKMVGPDGEESPSSIGV
                     FLIWQQPHPIYYAELLYRYHQDPVILEKYKDIVFSSAEFMASFVQWDAENGRYILGTP
                     LIPAQEIYPPDITLNPGFELSYWRFGLETAQKWRERLGLKREEKWDTILELLAPLPQN
                     NGYYQNAENAMNTFENSSHRKDHPTLLASYGMLADKTVDQKVMEKTLEKVMQSWDWET
                     TWGWDFPLIAMTAARVGRADLAIEALLMDVPKNTYLLNGHNYQNESLPVYLPGNGGLL
                     TAVAMMAAGWDGAPDIYAPGFPQDGQWSIKYENLVPLP"
     gene            complement(14798..16672)
                     /locus_tag="PKX09_04400"
     CDS             complement(14798..16672)
                     /locus_tag="PKX09_04400"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOU17095.1"
                     /translation="MKKKLLFSVLLLVLLALTVNAQNFSITIDAEKDAFYDALTGPSN
                     GLVFMPYLCYLRDIGTPPTDNADLSAKVWFSYDADYLYCYAEVKDDIVAATNTERFQN
                     DCLELKFDPDPNSGTNTKTANCRITAKGADNAEEPTGVDNLNGSGHLEDVEGTDYVVS
                     ESDYARRLTDDGYVLEFRVSFDYINEPEDNRFMVERTVGNKFGMAINIGDNDGTTREH
                     MIQWSAGHTDGAHSYPALCGTATFMDNHVLKLEAVGPRPQDTVVVNPNAGWYIDPNFA
                     VAIDAEKDAFYSELTGPSNGLVFMPYLCYLRDIGTPPTDNADLSAKVWFSYDADYLYC
                     YAEVKDDIVAATNTERFQNDCLELKFDPDPNSGTNTKTSNCRITAKGADNAEEPTGVD
                     NLNGSGHLEDVEGTDYVVSESDYARRLTDDGYVLEFRVPFDYINEPEDNRFMVERTVG
                     NKFGMAINIGDNDGTTREHMIQWSAGHTDGAHSYPALCGTATFMDNHVLKLEAVGPRP
                     QDTVVVNPNAEEWYTKDPTAGIKGQPPVAESFNLLTNYPNPFNPSTTIKFSVPKTAFV
                     TLKIHNTLGQEVARLVAQRLNEGVYTVNWNAAEFPSGIYIVRMVAGDLINTSKMVLLK
                     "
     gene            16951..17844
                     /locus_tag="PKX09_04405"
     CDS             16951..17844
                     /locus_tag="PKX09_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOU17096.1"
                     /translation="MIRTKYSIFDYLIISENDKNWGLYVTGSGNADIPPHTPYPPTKH
                     PKGYMFDWQNGRILTEFQILYITRGSGIFESKATGRKKIEEGYIIHLFPGVWHRYMPD
                     PKTGWKEHWISFNGSIPNDLLNNGILSREKAVLEIGLNEKLINLYQEILELIESQKIG
                     YQEIIASLTFQIVANIQAIEKSKKFFGIEFETTINKAKIFMADRIDKNINMEKLASEL
                     GVGYSWFRRMFRHYTGLPPVQYFLELKLNKAKELLVNTSLPIKEIAVITGFESQFYFS
                     KFFKNRVGMSPIKYREFSRGK"
     gene            18092..20767
                     /locus_tag="PKX09_04410"
     CDS             18092..20767
                     /locus_tag="PKX09_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOU17097.1"
                     /translation="MRRFMAVLLLGSNILFAQMPADTINPPDYLNSNLPVSVRVDDLI
                     ARMTLEEKVSQMVNNASAIPRLQIPEYNWWNECLHGVALSGIATVFPQAIALAATWNT
                     DLMFEIADVISTEARAKYHDYISKDERDIHKGLTFWSPNINIFRDPRWGRGQETYGED
                     PYLTARFGVEFVRGLQGNDPKYFKVISTPKHYAVHSGPEPDRHTFNAIVENRDLYDTY
                     LPAFEACVKEAGAFSVMCAYNRLYGEACCGSPLLLSKILREDWGFKGYVVSDCGAILD
                     IYQNHKIVATAPEAAALAVKAGTDLNCGQVYSSAIIEAVQNGLLSEQEIDTAVARLFT
                     ARFKLGQFDPPELVPYSDIPIEENDSEEHRQLSIRAAQESIVLLKNSDRILPLKKDLK
                     RIAVIGPTADSYQMLLGNYNGTPSRYVTPLQGIRNKVFEPTAVIYEPGCNLVEPGNFV
                     HNLSANVLSTEGQPGLKVEYFASQDPSGQPFFSRVDKIHGLNWSYGTTIPSFKGRENI
                     ASICWSGMFKAPTSGRYKFTIKSDSRVRLIIDNKIIIEDWKERKFASLSNSIKLKEGE
                     MYPLVLEFSRCGNRPQLFVEWELLNHNSFKNALDLAQKSDVVIFVGGLTAQLEGEEMA
                     VSYDGFYKGDRLSLDLPKVQEKLLKELHATGKPIVLVLTSGSALSVNWAQENLPAIIQ
                     LWYPGQEGGTALADVLFGDYNPAGRLPITFYKSVEQLPPFEDYNMRGRTYRYFEGEPL
                     FPFGYGLSYTNFAYSNLIIDEEIKAGEIIKVAVDVSNRGKIAGDEVVQLYVKNLDTTV
                     PTPIHSLQGFKRINLKPGETRRVEFLLQPRQLAVIKDQDMLNSENESSRKVQYIVEPG
                     AYEISVGGMQPVTISETTQIISKKIKIIGESYLIK"
     gene            20929..22446
                     /locus_tag="PKX09_04415"
     CDS             20929..22446
                     /locus_tag="PKX09_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17098.1"
                     /translation="MMNFQNNNDPSHGFYDLKLLLIILLFFVLFISGFSQNKPLDNSQ
                     IVAQIGNMTITVEDFLQRSELTVRPVYLKDKNTAINNLIAEKTLALEAEGSATEFLNK
                     PGIAGKLRGIKEQMMREALYYEVAFNKAQVDTTEINKVYRSSMREYELEFYVLKSKDL
                     AQKVQTVLDSMPELTDKMFEEFAGAIDKKPVHRINYNDNEDDVIHKALYSELLDTGTV
                     LGPLRLNDGNYIIMKVTDYIDYPLISSEDQRLRWNKVQNKLHQAAASKLWQEFQADLM
                     HGKKVEFDPNSFEVLKNLAFEMYLSSDATDSINFQIAEIVPSKPEINPNAPFFRIDDQ
                     TWTIDDFKRELLAHPLIFRTRNLTKDNFAEQFKLAVVDLIRDYYLTQESYKRGLDQSP
                     EIDRTLAIWQDALIAQNQIKTVLKEAVSNGIINDDPEELAKYRETYIKKLQAKYSSNI
                     RINLKVYEQISLNKVDFFAYRPGMPHPTTIPAFPVLIQSGDLTYAKQSIDSTKHK"
     gene            complement(22530..23546)
                     /locus_tag="PKX09_04420"
     CDS             complement(22530..23546)
                     /locus_tag="PKX09_04420"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOU17099.1"
                     /translation="MKSKIFLFAGLLLATSQMFAGVTKVGTTAAGFLMIDVGSRANGM
                     GSAFVSIADDATAMYWNPAGIAGIDGFEATFCNSKWIGDLGFTYTGAVQSLGQLGNIG
                     INATFLTMEEIERTTIAYPDGSGEYFDAGCYAVGLSYARKLTDNFSIGFNTKLINERM
                     YHSSASGFALDIGALFDTQLSGLKLGMCISNYGTKMHLDGRDLQVQHDISESISGNND
                     LINARLQTDYFDLPLLLRFGVSMDVLKGFMNSNLILSADALHPSDDTEYINLGAEYKL
                     FNLLSLRVGYKQLFAKDSEQGLTFGGGINYKLRGATAFYFDYSYSDFGLLNSVNIFSI
                     GLAF"
     gene            complement(23600..27076)
                     /locus_tag="PKX09_04425"
     CDS             complement(23600..27076)
                     /locus_tag="PKX09_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17100.1"
                     /translation="MNKKIFILFIISGCLFTSGLFAQMTYLHGTDNEFRMGLHAGNQF
                     RTTFYNDGTYGAINPWTPDEIIGEWPINSSHYYLMDGNIFVGSEVIDTNNQLIHILSE
                     NKSYNTGGSTGDKRPDGSWRTFLPLPGFANQSGDRIAMAKGGSEWPDSWPSYWPDIAD
                     PKNPYGIYNPDGWAGCWNGYFGKNVFNADEESYFVADDYENDEFAFFPDANNPNRRGL
                     GLRIYVRGFQWAKAPVEDGIFCLFDIENIGTHLHNKMVFGYKIGNNIGMCSENEQDTG
                     DDNAAFDRELDLAYMWDNDLIGGACWGPDPVGYMGGAFLESPGNPYDGIDNDNDGISG
                     SGKTISEDLFAPKTLSLGDQIVFINYDTYDRIVTTLGDTLTRLGKNSTDTLSITFNNA
                     TFRYWAGKTLTEDPTGSNLFDDNLNGLIDENRKPEGATQALYLGYKYVDYFTGEGLNN
                     PLIDERRDDGIDNDNDWDIIYDDLGVDGIGPNSRDYDGADIGEGDGQPTAGEPHFDKT
                     DIDETDMLGLTSFNLYEWTSINQNDDEAYWQAMAPGYFSTNMTGSNVELLYGCGYFPL
                     SPGQVERLSIAIICGTNYNDLVRNKNNFADAYNRNYNFAKAPNIPTVRAVAGDNKVTL
                     FWDTFAEDSDDPITGKDFEGYKIYRSTDPGWNDCTPITDGYGSVIFREPLAQFDLDNG
                     IYGFADIPTQGVHFWLGNDTGLRHYWVDTTAVNGYTYYYAVTSYDHGTQQPSGSLIID
                     SLKIDPSECSKFISIAASGTVEKGSNVAIVRPEAPSAGYKPAEMESGIIPGPNNTATG
                     TINYTIYDNTNIKNNHQYQITFKDSLSGTGVTATKSTKSFTLVNTTTNDTLIYDHSLE
                     GGAEGLPIVDGFQLSFANNPEKLEVDMDRSGWNTPGIPEFRFRPENRAKPEDLYTSDF
                     KIAFRDAPDPSLPKPIFYYKGSSSKLYEPIPVNFEVISLKENKNIPFAFREKHATTTA
                     DSGIFSYDLTSTVKYDEIIFLSPDSLPSWKAIYYITATTKDSLVPRTGDTLTIRLKFP
                     FLSHDVFEFTSKIDTIDVEKAKNDLSKIRVVPNPYVVTNTWEPKNPYSSGRGERQLHF
                     IHLPAKCTIKIFNVRGQLVDTIKRDSQLDDGTEIWDMLSKDNLEIAYGIYIYHVQAEG
                     IGEKTGKFVVVK"
     gene            complement(27095..30232)
                     /locus_tag="PKX09_04430"
     CDS             complement(27095..30232)
                     /locus_tag="PKX09_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU17101.1"
                     /translation="MKDRVIFGIWLLLISILLNGILFGGTTGKITGVITDASTSAPII
                     GANVILKGTTMGAASDSEGNFTILNVPPGTYTVVISMIGYQQIQFEDVRVSIDLTTRL
                     DATLKQTALDLGESVIVTAERKLVLRDMTSSLSTIDADQISNLPVQQIQDVLRLNAGV
                     IESDGRLHIRGGRAGEVAYWVDGISATDVYDGRIGINIENSAVQELQVISGTFNAEYG
                     QAMSGIVNIITKEGGQDYTGYIKVYGGDYVSDDNVFGLYKRILYDSTRSNILSKEWEN
                     PLKKINPIYNGEFSLGGPVPLFKDNLSFFINGRYYYDEGYYYGRTWYQPNGAPIDSTV
                     LTSQMKSLYAANGITFGGSSLVPMNPMERTTFQGKLSARLSRSMKLNYNFFWNESYQE
                     RAYFRINTNDYQFNPTGTANYQQFNTHDYKYNPYGLPQIHNQGMAHTLTLNHIINPSN
                     FYELRISRYESKSKQYVFDNPNASNDYLVYVKEDTSKDIIAEIIDPFTERGAIKFDSL
                     KYLGAQYEYIVNPEGSVGYIDPDDITAPTSYSFMNSGMDVTRANRKTAYWVGKLDFTS
                     QINKYNQLKFGSEVRLYELTLHSYQIVAATNESGQELTPFQPAIPEVGSVYRNDYFRE
                     PKELSAYIQDKLERNNIILNVGIRYDYFDANSVILADPIDPNIYSPFKYSNKYANWID
                     MPNNYRGTIDQWVDSLLASGTIREYTPDERRAFMHKKVKAKSSWSPRLGLAFPITDRG
                     VIHISYGHFFQVPEFQYLYTNPDFKVTTGSGAALFGNPDLKPQKTVMYEIGLQQQFGE
                     NIGIDLTLFYRDVRDWVGTSPIIQTVKTGVSYSQFVNKDYSNVRGVTFKIEKRFSQNY
                     SFRADYTYQSAEGTYSDPTDAFNSALNNEQPVLALVPMEWDQRHTVNAQIIYDVSGWT
                     FSLIGRYWSGRPYTPSFPSSETVGGAAVSGLTTNSARRPSQRNIDLTLNKILMVKSKF
                     RLELFANIYNVLDIRDELNVYTDTGTADYTTTINPKKVVYSPDRVSTVEDYIKQPTWY
                     TSPRQIQVGLAIGFN"
     gene            complement(30698..31021)
                     /locus_tag="PKX09_04435"
     CDS             complement(30698..31021)
                     /locus_tag="PKX09_04435"
                     /inference="COORDINATES: protein motif:HMM:NF019139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HOU17102.1"
                     /translation="MTYTYQDLKTKTVAQLREIASQLDSEAVKGYTQLNKEHLLAALC
                     QALQIDQHQHHQIVVQNKTEIKSKIRELKKERDKAIANHDRAKLIRVREQIRSLKHRL
                     HRSMV"
     gene            complement(31162..34230)
                     /locus_tag="PKX09_04440"
     CDS             complement(31162..34230)
                     /locus_tag="PKX09_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HOU17103.1"
                     /translation="MKKSILIIMLSTISLFGQIPGFTDDFEDGSLDTLWNDSLMTLWH
                     SDWYPGIFNLTESEGVLEVAYDRKSTDSDWPAFYFTPPEVINVAHNPKIVIEIKSDIT
                     INFAIKPIYTNGKDNYQSTTVPGSNTWQTYTFTLSNYAGGYCNQVVFHFDGGTKVDKQ
                     GTVCFGNFIIAGFSISIQGLNAKFDPINQYVDLVWSCDNEAAVDHYNVYRDTLKGFSC
                     TTENLIGEPEGKNWRDTQIGIGTYYYKVAAVDTMGKQHAVSMEMACRTYTIGAIPVVE
                     IADVNSSTVGKYDKFEITLKLTEASYGNPYDSDEIDVFANFISPLSDTIRINGFFDNY
                     MDIGEWKIRFAPLVIGQWEYQVFARDIDGVGESAKCNFTVVESDYHGCLNVSSVNPHY
                     LIYHDGTPFYGIGAYYPWGVRNDDDGLGILAASGGNLFGYWNGNYDGAGNGGGIYQIE
                     SIQSGIGYYDQRKCARIDEILDWAEQRNLEMMLAIWPHDILDQSTWGYKGWDDNAYKT
                     VCNAANFYTDSLAWVYQQKLYRYMLARWGYSRSLGIWELVNEIDGTDGWSRGNKTGAL
                     EWVQKVHDYLKENDYFKRPTTISKSGSSANYWPEGYRICDLPNVHLYEKGWSAPYASD
                     PIRSSYWTYRNVARQLWTGFEKPGIFGEAGAEPTNMYAANIEKGSSKYVEIYHNALWS
                     AWASGLAATPIWWEMNDRELMTDEVFAQMKAFARVASDIDYADLNLISSNASADTADV
                     FMMAADSLGFGWFRSYNNNAISGRFTRISGLKTGTYQAIWYDTWAGDSVIVGNCVSAG
                     NMIYTEIPEMPTPCKDIALRLTKTEDGTQPASLKIFIDKPIITSFPVTNYLIICYVSD
                     TENRLCADSFQISLTLEGPGIISNPQVTTGNGVAIFNYLPEQGKSGEVVFTASTEGLA
                     SVSLFNNVLSEIEVERNASIPTEFALKANYPNPFNASTVIEFSLPKTTNIKIDVYNLA
                     GQLVETLVRGRQSAGNYKIFWHPANLPSGIYLYQITCPEFTATRKCMILK"
     gene            complement(34285..35442)
                     /locus_tag="PKX09_04445"
     CDS             complement(34285..35442)
                     /locus_tag="PKX09_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HOU17104.1"
                     /translation="MLTDQDAWLNLSNQACPSQNGIYQRYRNPVLTAKHIPINWRFDL
                     NYATNPFLMERLGVNAVFNCGAIKFNDKYCLVARVEGLDRKSFFAVAESPNGVDHFKF
                     WEYPIIMPETEIPDTNVYDMRLTHHADGWIYGVFCTERKDPNVPEYDTSTAVAQAGIA
                     RTHDLKKWERLPDLQTKSRQQRNVVLHPEFFDGKYAFYTRPQDDFILAGTGGGIAWGL
                     ADNIEKAVIDEEIIIDSRVYHTINEVKNGEGPTPLKTAEGWLHLAHGVRNTAAGLRYV
                     LYMFLTDLNEPWRIIHKPGGYFLAPEGEELIGDVSNVVFSNGWIADPNGRIFIYYGSS
                     DTRTHVVTTTIERLLDYVKNTPEDGLRSAKCVEQRVALINHNRNIIDNFKK"
     gene            complement(35492..36712)
                     /locus_tag="PKX09_04450"
     CDS             complement(35492..36712)
                     /locus_tag="PKX09_04450"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOU17105.1"
                     /translation="MKNDAIANWRQQVSRNLYENIIPFWMTYSIDRRNGGFYGRITND
                     LQVDKTAPRSVVLIARILWTFSALFRFDKDSRHLEMANRAYNYLTKYFSDSKYGGVYW
                     LVAPGGEVLEDKKKIYGQAFAIYGLSEYYAASGEKAALEMAHQIFQLIEKYCHDAING
                     GYYEAANRDWSETQDLRLSAVDLNEKKSMNSHLHLLEALTNLLRVSSDSLVRARLQEL
                     VECHLRYILSPVSNHLYLFFDEKWNPRLNGVSFGHDIEASWLITESAEVLGDDKIQAR
                     VKSAIHKIVAAVIAEGFNQHNALPVANDMAGSLSANNFEWWQQAEALVGLINAYQLYG
                     DSSYLDRAGRLWDFIDKYFVDWQRGEWYYEISAEGIPNPAKPKVSEWKCCYHNTRACL
                     EVLKRTAQLINRRE"
     gene            complement(36753..>37405)
                     /locus_tag="PKX09_04455"
     CDS             complement(36753..>37405)
                     /locus_tag="PKX09_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOU17106.1"
                     /translation="DIYLKYMRPKATNRQVSSVTYISGIGIVVISIIFGMFALDINSI
                     LQWIVSALWGSYVVSNVLKWYWWRFNGYGYFWGMVAGIIPALIFPQIFDTLDLYYFPL
                     ILLISLAGALIGTYRHPPTDEAVLKKFYKTVRPWGFWKPIHEKVVAEDPNFVNRSSWK
                     RDLLNVLIGIIWQTTLVALPIYFVLKKFGNAGICLLIIIVTSIFLKKNWYDKMEDD"
     assembly_gap    37406..37505
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<37506..38731)
                     /locus_tag="PKX09_04460"
     CDS             complement(<37506..38731)
                     /locus_tag="PKX09_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOU17107.1"
                     /translation="MRLKLIDIVIILLYLSFTVVIGLVLKKRAQENKTAYLLGGNKLP
                     WYMLGISNASGMFDISGTMWLVTLMFVYGFKSIWIPWLWPVFNQIFLMVYLSAWLRRS
                     NVTTGAEWIGTRFGSGRDATLSHNIVVVFALLSCLGFLAYGFIGLGKFMEIFIPWEVV
                     SNYVPFSVPDKFVPHLYGIVFTTFSVFYTILGGMVSIVWADVIQYCIMGVSAIVIGII
                     AMRALSIHPLIVPEGWMSPFFGWELNMDWSGIIAEVNDKIASDGYSLFMIFFMMMLFK
                     GILVSAAGPAPNYDMQKILATRSPKEAGMMSGFVSVILNPIRYIMITGFAVLGIIYYD
                     RLDLIVGGRLDFEQILPSAISEFVPIGFTGLMLAGLLAAFIGTFAGTLNAAQAYIVND
                     IYLKYMRPKATNRQVSS"
     gene            complement(38785..39615)
                     /locus_tag="PKX09_04465"
     CDS             complement(38785..39615)
                     /locus_tag="PKX09_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HOU17108.1"
                     /translation="MKKLFRFYIVLIIIVSLAFSQEKNKVDLSIGANFIATKTYAPEE
                     DARILKLFEGLRVADVSDGMDMVGLPGVGLVDPAIHSDWLDLEDLSHQIRGIAITARY
                     VPTQRPDRPQAGEKFQEWEGNFYNVYSPETFAELIRPGSVIVLDDVEEKDIGTIGSYN
                     ILAWHKSGAVGVVTDACARDTDEIAKEKVPLYLRKKGRGIRPGRNELESVNRPVVIGG
                     VLVCPGDVVVADGDGVIVVPRNVAEQVAKYAREILVKDKAGRKDLYKSLGRPLDKTVE
                     "
BASE COUNT        11820 a         8882 c         7519 g        11489 t
ORIGIN      
        1 ttcttggcca tactttcacc atctttgccg gttttaaagg tggcaagggt gttgccgtcg
       61 gagccggaat gattatcgtt ttatatcctc ttgcgctttt accatgtctg ctggtcttca
      121 gcattgtcct attctcgacc ggtatcgtat cggtctcgtc aatcagcgcc gccctcagtt
      181 tgccgatcgt tttactgttt ttcaattatt tcggaacttc gccgatagat aaagcactcc
      241 taattttcag tatttttatc ccaattttta ttgtttatac tcatcgtgct aatatacggc
      301 gactttttaa aggtgaagaa aagccattcg aataattaat tctcctacgt cggcggcgat
      361 cttctaaaaa gtaacttttt gtttaggatg aatttgtcaa cctatcggtt ccgtctcgca
      421 attccgcttt tggggctgtt attcctctcg ggctgggctc aaatcccgtg tgccgacttt
      481 gaaattgtcg aaagcattcc gattgagact attctcgata atcccgaaat tcgtaacact
      541 tctgaagtct gggcagaaat gatcggcgcg gctgatgaga ctattgacct agaacaatat
      601 tatatctcga acgcagttga tgaaccgctg gaaccaatta taaagttgat tgaacaagcg
      661 gcgcgacgcg gtgtcaaagt ccggattatc gtcgatgccc ggatggctaa gacttatccc
      721 gaaaccatta accgacttac taaaacaaaa aatattacta ttcgccaaag tcagactttt
      781 aatcgcaatg atggcgttca acatgctaaa tattttatta ttgatgggaa ggaagtcttt
      841 attggtagtc agaatttcga ctggcgagca ctcgaacata ttcatgagat tggcttgcgc
      901 atttgccatc cggaatatgc acgtctgatg acagaattgt tcgaaatgga ttgggattat
      961 gcggtattga ataaaatgcc taacaggaaa ttgactgaac cacatcgttt taaagtagca
     1021 attgctcctg gtgatacagt taaattcact gcttccggta gcccagctag gaacctacca
     1081 gacggaatga cgagcgacga aaaagtcatt ctggaactta ttcgttgcgc aaaaaaggaa
     1141 attaatattc aattgctttc ttattcaccg ctggataaaa agaacagcta ttgggcgaaa
     1201 ttagataatg ctctgcgcga ggctgcggct cggggagtca ctgtcaattt gctggtttcc
     1261 gactggaata tcagtgctaa cgaactacct tacttgcaaa gcctcgccat cttgcctaat
     1321 atcaatttaa aaatcagtag catcccagaa tatagcggtg gctatatccc ttttgcccgc
     1381 gtcgaacact gcaagtatat ggtcgtagat gaatccctta gctggctcgg caccagcaac
     1441 tgggcgcgca attattttta cgattcacgc aacttgggct tgaaaatcga aaatgctacc
     1501 ttcgctaaaa ctctccgaaa tatattccta aaaagctggg attcacccta cgcctggcaa
     1561 atcattccag ggcaggatta tccagaaaaa tttcacgggg agaaataagc cgcttaagta
     1621 tacgtaggac aggcattcct gcctgtcgga taaagacaac ctggaaaggc tggcatacca
     1681 atttatacca ttttcaatcg taggacaggc attcctgcct gtcaactata ggttgacgat
     1741 gacagcctag aaaggctgtc ctacgagatt aatatctaat ttcgagttgg attgagcgat
     1801 tttgcggaca ggcaatttca gcgattttac tttgtttatc gtaattaaaa gttgccgttt
     1861 tgccgccgta aaatactttc tgtggctttt ccgaacacgt aaccctgaca actgacggga
     1921 ttggaatcgc cggtgaagag aatagtattt tcccttcttg atgttggagt gtcattgttt
     1981 tttctgtatc accgtccaga taatcaacgt aagtaaattg ggtattttcg ttgatttcac
     2041 cgggatagag atgaattatt aaattttctt ttccattcag gttgcctgcc caagtttttg
     2101 aatttccttg atagatttgt cctgaaacat aaattgaatt ttgcctaata aataccggta
     2161 ttcgttctaa tggaattgat tgactaattg tcaggggccc tgaatattct ttattcggtt
     2221 cataatagtc aatccagttc ccttgaggca agtaaatttg gcgctcatta tccgctgaaa
     2281 agaccggtgc aatcagaaaa gccgagccaa aaataaattc atcccaaacc gtgtaggtat
     2341 tttcatcatc gggatacata taagccaagg gcttcattag tactccgttt ttataactgg
     2401 tattagcaca ggaataaata tatgggagca tttccatccg aaattgacaa acattgcgga
     2461 aaacctgctg aagttgttca gagtatttcc acggcggacg atcctcaccg ctcccaccag
     2521 cgccgtcaat tttaatctcg aacatcccgt tccaaacacc ccactccaac cagcgcatat
     2581 atagcagttc gtcaattttc ccttccccaa gatatccacc ggtatcattt ccccaaacgg
     2641 gaaaacccat aaagccacat cgaattgcat tagccatatt gcccgccatt ccataccaat
     2701 tattacggct gtcaccaccc cacaaagcgc tcagatatgg ttgagaccga tgaaaagccg
     2761 ctctggcaaa gttaaaattg tcgcggccat gggcattctg gagaaattta tcaatcgttt
     2821 tagcatacag ataaacatat ttattgcgcg cttcagtctc cgtaacaggt tcataccagt
     2881 cagcggtcaa ggggaaatgt tcatcggcgc gatccatttt gtgtcccttg ataccaaccg
     2941 catattggtt cgatgctaag cgtcgttcga attccgccag tgccataggg tttgttaagt
     3001 caatgtaatt tctagaatct ggaaacaagc ctgggaaatc actatcggaa aaagtcagtg
     3061 gcccaatcca ggtcattact tgcattctat agtcattatt aatctttttg atccacttct
     3121 ccggctcggc aaatttatcc gaaaaatcca tcttcgacca ctcatgagca ccgttgctat
     3181 aaggccgatc aataaagcag gcggtcaagg gaatttgtag ctcattgaat ttttgaatat
     3241 cagtaagcaa ctcgtctttg ccgccattat tttgatcgcg ccagaaaatc gggccgcaag
     3301 cccagatcgg aatatatttt ggtttaccga tcagacggta atattcacta tggatttttt
     3361 ctccggtggg accgtagaaa atgtaccagt ccagcgttcc ggtttgatga taaatctcgg
     3421 tgattccacc aattcccaat ttgtatttcc ccttggcaaa ggtatcaaaa aaactgccgt
     3481 aaccttggct acttatgaaa aaagccgaat aagcggaagc ataattttcg gcgtaatcca
     3541 aattaccaag gccatacaca tcaacgtcaa cgacattgcc tctcaagtca ggatttttca
     3601 aattccccgg atacagtttc tcaattaagc cgaaataatg ctcattttga tcttttaagc
     3661 aaactgtgat atgattaaaa gtctgatgcg aggtaaaaaa gtggaaagta ttttctgact
     3721 gcgtgatctt taaattaatt tccgaaggga aatccgcatg gaatccatcc ttttccgcca
     3781 ggcgtaattt caaaattaat gaatcctcga aaacctcttc aactataata gtgtctaccg
     3841 gaataaaatt gaaggaaaaa gaactaatct cgacgatttt atcttggcta tagaataaac
     3901 tcaatacatt attctgcaaa tgaactttat attggttttt ctcccagatt acctgagcaa
     3961 ataatgattc cgccgaaagg aatccaatta ccaataaaat tttagaccat tttttcatca
     4021 gataattcct accaaattta aaattgtttt tgcaaagtta gaaatcgttg ataggctccg
     4081 tcccagagag cgctatcttt aggttcatac cgttttatgc ccctcatttt ggcaactatt
     4141 tgtcgtgctt cactcagatt ttttatttct ccgagagtaa ttagttggac aatggcattc
     4201 cctaatacgg tggcttcggc tgggccggcg aacacggaca agccggtcgc attggccgtc
     4261 aattgattaa gtaaatcatt ttgtgatccg cctccgacaa tatggataag ttccaccgga
     4321 cgattcgata ttccaaccaa tttctcgagt acattcttat aatctaatgc cagactgtct
     4381 aatacacacc gtacaatggc tcccggagta gtcggcatcg gttgggaggt aatttggcaa
     4441 aaattggcta tcatctgagg gaaattccct tgatgtaaaa attggggatc atctggtata
     4501 atgagcgacc gtaagggcgg ttcttgtttc gccagtttta ctaaatctga actggtataa
     4561 ttttccccct ctttccgcca ctgtcgttga cattcttgaa ttagccagag tcccataaca
     4621 tttttaagga acaaaaatgt gccgaatacg ccaccttcgt tggtaatatt agcctttaga
     4681 gctgattcat tgattagcgg agtcgcgatt tcctgcccaa ccaggctcca ggtgccgcta
     4741 ctgatatagg taaaattttt aatttcggtc ggaacagctg cgaccgcact ggcggtatca
     4801 tgagccgcta ctgtcattac atctatgccg tcatatttac ccagctgaat acctggtgaa
     4861 ataacatccg gaaaaatatg atatggaatt tgtaatttat taattagcga ctcagcccag
     4921 tttttttgca gtggattgta tagctgagta gttgtcgcgt tagtaaattc acacgctttt
     4981 accccaacga gccaataatt aaataaatcg ggaatcgtca ggaatgtttc cgcatttaat
     5041 aaaaccgggg attgcttctc gactaaataa cttagctgaa atagggtatt gatgggcaat
     5101 atctgaatgc cggtttggga ataaatttcc tgtttagaaa ttctggcaat gaccttttcc
     5161 tgcataccgt ctgttcttcg gtcgcgataa tgaaccggat tatctaaaag aacaccttgt
     5221 ttatcgagca acgcaaaatc aactccccag gcatcaactc cgatactgat tggtttaagg
     5281 gcttttcctt tttcaatccc ggctttggtt tcctgccaga gtcgtgaaat atcccaatat
     5341 aaagtcccgt catttattat tgggacattt agaaagcgat gtaattcctt taattcaaaa
     5401 ttattcccgt cataatttac cgccataacg cgtcccgatg cggctcccag gtcaacggct
     5461 agtacagtta aatttgatcg catttaaatt tagatgaatt aaaagattga atttaaacgg
     5521 gatagccatt attggcttca gagattcctc tttcacttat aattttattc cgataattat
     5581 ccttgcggta ggctaataaa ggatcgcgcg gcactcccat ttcttcccga acctcctcga
     5641 gtaatggata tacatccgtc tcaaaggctt tggtcaaaat gctctgcgct acgagaatat
     5701 cgccattcat ctgtgcttct tgaagagccg ggtaatctac aatcaacgcc ttggccaagg
     5761 cagtttgaca atttatgata ctcattatca tcgcttctat cttagtttcg atcgtatgac
     5821 tttgatcaat catatacacg attttatcgg ctttcgaacc ggctgaaacc aatttggtaa
     5881 aaacatcgaa aatttcaaac ggattaacag tacctacaat taaatcatca tctgcatatt
     5941 tccgggcatt gaaatgaaat ccgcccagtt taccttcact aagtagaaaa gccacgatat
     6001 gaggaatatt cgtaccgagg gcatggtgtc cggtatcaac caaaacatca gcttgaggtc
     6061 cgcatttcat tgccataacg tacgccattc cccaatcagc tagatcagta tgataaaaag
     6121 acggttcgaa aaatttatac tctattaaca tccggcagtt ttggggcaaa ttggaatata
     6181 tttccttcaa gccggattca agattagcct tacgtcggaa aagatcatcc tgtccggcat
     6241 agttggtacc atcagccagc cacaagctca aaacgttgct cccggttttt tccataattt
     6301 cacaacaagc taaaatatga tctatggact tttggcggac tgctttagag ggattgcata
     6361 aggagcctaa tttgtactgt ttatcctgaa acaaattggg atttatcgcg cctactttaa
     6421 tccctttgga ttcggcatat tgtttgagag cggtataatc atcggtttta tcccagggaa
     6481 tatggagtgc tacggcggga gcaataccgg tcaatttatg aacataagcc gcatcggcaa
     6541 tcttttcgta aacattcctt gccgctccgg cgcaggcgaa tgtattaaaa cgcgtcccac
     6601 tattaccgta tccccagctg ggagtttcaa ttcgctgact tttgagtgcg tgtatgactt
     6661 tttcaaaatc aatatgggga ttatcaaaaa tattgctcga ttttccggat ggattcatca
     6721 ctttctccaa ataatattat caactttcac ttccatgttt tctatgtcaa tatcaacttt
     6781 aggcctcatc cttcttttcc atccaaaagg gcgctgatac gaaccacata atagttccaa
     6841 cattcattaa taaaagataa gtatcccatg aaatagcgtt ggcaaatacc aaaaatgacg
     6901 gcaaaagagt caaaaccaga ccaatgagtg atacgattac caaaattatc ttcattactt
     6961 ttccttttct ttttttgcaa cgagaacttt ttgttgaaaa tagcttaaaa cggtgtataa
     7021 aatgatcgca ataaaccatc ccggcaaagc cttgaaataa atttccatcg gcaataaaaa
     7081 tgcaattaat aaagttatac accaggttaa aaataccggc cagctgaact gaattttcct
     7141 cacctcggca taattctgag tcagtttcaa tttaggaaat aaccagaaat cggtgaaaat
     7201 tactgctccc atcggcatca aaatcaaccc atagagcgac acaaaatcca gcaatctcat
     7261 caccagcgct ggaaaaaaag ccgcaaaggt cgtgatggcg ccgatgatca tcgtcacttt
     7321 ccaggtttta gattttggca tcactgcctg aagtgcgagc ccagcccgat atatggtcgg
     7381 attggcggta gtccaaccgg caatgattac gcaagcgatc ccggctattc cggcagcacg
     7441 ataagcaacc tgccccggtg aaaatgccag actattattt gattccaata aaaaaagcga
     7501 atataaaatt cccgatgcca gccaggcaat gtaatgcccg acaaacatac cagccgaaga
     7561 agtaacgcca taataccact ttttggcata acgcaaaatg gatagatcag ccattccgat
     7621 atgcatcgct agattacaaa accaagagaa aaatataatg tgccaaatgg tgaatttact
     7681 ttgaccttcc aggggaacgc cgttccagat tctttccttg gcaacttccc agaaattgga
     7741 aaaagaacta actcctattt ccggtaaaac cgctacggct gaggcaataa ataccaaagg
     7801 catccaaggt gccgcaaatt tgccgaactt ggcaaccacc gaatagccga acattgccac
     7861 cgcagttgtg acggcaccaa ccacaatcac cgccacaatc cagccaatac tattggggaa
     7921 aaagtcattt aaagaaggca ttggcagatt aaatggaatc ccaatcgccg tcgctgatac
     7981 ggctatcatt gcgcctgcta aaccacaaaa cattagagca ttgaccagat tatacaacaa
     8041 ggtcactttt ttaccggcga ttttttctaa aaaataatag agggtaatac gggctctcgt
     8101 cgctatcgga ccggtaatga atgtccaact caaaacggct aaaagatttc caatcagcaa
     8161 tccagttact agatcaaaag cgctgacgcc atgggcgaca aaaagtggcc caatgacaaa
     8221 ttcggttccg gctgtatgtt caccggcata cattccccag aaactgcctg ccccttttaa
     8281 tttcgaggga ggcacgggag ttcgttcgaa ctcattgatt tcatctagtt tctttactaa
     8341 aacgttaccc cgtttagact tcatgcgatt tctccaaact ttgatatcga atatctactt
     8401 caatcaccaa atgaacgttc ctaccgtaat aataccattt ttttgagcag gctgggttta
     8461 tccccgattt tcaaacgata aaaatatatt cctgacgccg aaggtatgcc gttctgatta
     8521 cagccatccc acctgatctg gttgatgccg ctggaaaaaa tccgagggct aagcgaccgg
     8581 attaatctcc cattcaagtc aattatctca atatttactt cacctaattc cgctaaataa
     8641 aagccgatat tagtagcgct gttaaaagga ttagggaaat tctggaaaag gttatagttc
     8701 gtcagaagaa cttgcttcgg attattttta atgtcagaag cgcttcgggc aattatcgtc
     8761 attgtatcgg aggcaatttc gacatcatta taaaatacga cactgtaata gcgattcaag
     8821 gaaccatagg aaatgaaact cgtcatctcc agtggtttgt ccgagccata attggcgtat
     8881 aattcaaatt gtccatcggc cttcgactgt ttgtagattt ttatatattc accgatgttt
     8941 gaggtctttt gcgtacgcac ttttacccgc atatatttgt cggaagtcga gactaattcc
     9001 cagccccaga tgggagcatg tacaactcgc ctctcgaaaa tatttatctg gttaataagt
     9061 aaaccagagt cgtcgggaat tccattttct ttaatctgaa attttagaaa tcgagccgga
     9121 attggttgaa atgtcttact gaataatgcc gtactatcac attttaataa cgaggcacta
     9181 agtaaagtag tgtcaattag cgttgtccaa tcgagcgaat caatcgaagc cgcaaaagta
     9241 aaggcaaatt ccgaaattga aatattggta aaattaatct gaactaaatc aactttaacc
     9301 tcatcgccca ggtttattgt aaccggcgtg ctggtagaag aaaatacagc ataagtattt
     9361 gaattcccat cagtaagttc tggtacattt gttccggatt gaattgccag tttgcaaaga
     9421 gcgacgtcat tttcggtctg ggattcaatt cttcgaccgg ttaattcctc cataagtatg
     9481 cgggctatca aatcagaacc ttcgattagc gggtgaattc catccggcat aagactgctt
     9541 ttatccagaa atggtgtgtg gagatcaatc agattaacat tggctgagtc ggcaacttga
     9601 cttatcatag ggataatatc attgacgata atcgaatctc taatatccca cgcaccactg
     9661 aaagccggga gtggcaggca aatataaatc tcaggacggc tggataactg ccggaaagta
     9721 tcaatcatcg cgagataatc ggaaataaat tcacctttat aaatccagtt gtaaggtttg
     9781 ctatcgttcg tacccagcat tatcgtgaca atgttcggat tgaagttgag cgcctcggca
     9841 aataattttt cgttccatat cggataatcg ccttttttca ataatgtcgt gccggaaacg
     9901 ccacaatttt ttacttcata cccagcgcct aaataacgcc ctagagccac cggataagca
     9961 tccagagtct tagttgccat agcgttgcct tccgtaatgc tatttccgac gcaagcaatt
    10021 cggatgacat tttcttggga ataaagtaat tgagtaacta ggtttaaaaa aattgctata
    10081 caagctattt tttttgacag ttttttcttc attcaaatct ctctatctat catttgccga
    10141 tttttcctcg ataattaacg atatacttgg caatttctgc cagcggcgcg atccagatgt
    10201 ctttctcatt ttgctttaga aaattaacta gttgattgtg agcctccaga gatacattaa
    10261 tattatgttc gccgcccaca ccgtgaaaca agaatacgat taaagcctta ttttttaaag
    10321 cagtcttagc taggtcaatc aactcgactc ccgactctcc attgatcata aaacaaccga
    10381 tattgtataa atcaatgtca tcaattgttt cgagccgggt ttcagtacca cgggcaccga
    10441 cgaagttttc tttaattaca ttaatgtacg aaacaccacc gacctgcgta tcgccacatg
    10501 ggtaagcaaa tgtccttttt gatttcccat caataatttg caacagaaca ttagccattt
    10561 ttatttcatc cgttatacgc gaaagcgtat aagtgctgag gtcataatct ggattgaccc
    10621 aattccgacc gggcattttt ccatcacagg gatgaaataa agtatgatta ccgagttcat
    10681 gtccgttatc agcggctaat ttccaatcgt ttgcccgctc ttgaactgtt tggaaatttc
    10741 ccgacaaata aaaagtgcct ttaagtccca gagaatccaa caccggtatc accgaatcca
    10801 aatgcacgtt tagagcatcg tcataagtca ggcaaaccgc acatttttta ttattccacc
    10861 aattcttctc atttttctgc gccattccgt tctccctgaa aattgaaacg attccgaaca
    10921 aaatgataaa tcctattttt atatcgtaaa attttaagca tttcataaat cactccctat
    10981 aattattcga actgatcatt tgactatttc aataaaatca attttttagt actagaaaaa
    11041 tctccagcct gaattttata gaaataggta ccgctaggaa gataactgcc gtcaaattca
    11101 accgtatatt cacccggagt tttactctca tttaccaagg tggttacctc ctgcccgaat
    11161 aagttatata ttttcaaggt cacgaaagac ttttgaggaa ttgaaaaagc aatggtagtt
    11221 ttcggattaa agggattagg gtaattctgc tttaaataag gaatagttgg cacatttttt
    11281 aatcgaatgg cattgtctac gcttttcacc aggataatat cgccttcgtc cggaataatg
    11341 tcgaacatta gatacctggc tgtaccgatc tcaacaattt gggaattgat cttttcgcca
    11401 ttttgaaaaa tttcagccgc tgaccaacct tccggtaaga cgcggcgaat agttaacgga
    11461 acattataaa tttcattatc cagagtatcg gcaacttgga aagtaatact actatcggta
    11521 acaaccgatt cggtaattga tacattattg cgttcctgga tatagcgcgc cacgttggca
    11581 aaggaggata cccagaattt atccggatag gcacttagag aatcaaccgc accagtcaga
    11641 ttttccgttg tcactggcga ccaaccgtcg tcgttatcaa tgccatgaat tagaaaaacg
    11701 acccagccct tggagttcgc cgcgcttttg gctttattaa agaaatcgtt agcggttttt
    11761 actgaacctt cagtaccgca tacgatagaa ctgatattca taaaattact cggagttttc
    11821 ggctcaattt ggccggagca acctcgggcg gcgatgtagt attgcttaca aattgaagtt
    11881 ttgccagcat tacagtaggg ataagctatt gtgatgcatc gctgtccgct gatgcgagag
    11941 ttaatttcat tctgcgaatt ttgtaattct gtgatttgtt ccgcctccga taaaccgctt
    12001 aaatcagggt gagttacggt gtgactgccg acttcatggc cgtttgccgc ggcggattgc
    12061 agcttcgacc aaattccacc cgaccagttt attaccggat ataacgtgag tttgaatcca
    12121 tgctggtcaa agagtggaat agccaccgta aactggttgg cgcaaccatc atcaaaggta
    12181 aaagtgaccg ccgcggaacg aaatccctgc cagactccga cttcatagcc tgggtttatt
    12241 tctccggcat agacaatatt cattaaaact aataccatga tgagacaaat tcgcttgaat
    12301 ttcaacataa attactcctg ctttaaacaa ggctaaaatt caaattatta aaccgagaaa
    12361 tattatttat ttttttatcc ttaatgcaaa ttttatttaa cctacttatc ttagaaattc
    12421 tcataatgtt ttatggtaaa ggcactaagt tttcatattt aatagaccat tgcccatctt
    12481 gtggaaaacc gggagcataa atatcaggag cgccatccca accggccgcc atcatcgcca
    12541 cagctgtcaa taaacctcca ttgccaggca aataaactgg caaggattca ttttgataat
    12601 tgtgaccatt caaaaggtaa gtatttttgg gtacatccat cagtaaagcc tcaatcgcca
    12661 aatccgctct gcctacccga gccgccgtca ttgctatcaa gggaaaatcc cagccccaag
    12721 tcgtttccca atcccaggat tgcataacct tttcaagcgt tttttccata accttttgat
    12781 caacagtctt atcggctaac attccatagg aagccaataa ggttgggtga tctttcctat
    12841 gactactgtt ctcaaaagta ttcatagcat tttcggcgtt ttgataataa ccattatttt
    12901 ggggaagcgg cgctaataat tcgagaatag tgtcccattt ttcttccctt ttcaatccta
    12961 accgttctct ccacttttgg gcggtttcca atccaaaacg ccaatatgac agctcaaaac
    13021 caggattaag tgtgatatcc ggtggataga tttcctgagc ggggattaac ggtgtcccga
    13081 gaatatagcg accattttca gcatcccatt ggacgaaaga tgccataaat tcagccgaac
    13141 tgaaaactat gtctttatat ttctcaagaa taaccggatc ttgatgataa cgatacaata
    13201 actccgcata ataaattggg tgaggttgtt gccagattaa aaatactccg atgctcgacg
    13261 gactttcttc tccatcgggt ccaaccattt tcggccagcg tgctccggca tacccttgcc
    13321 gctcggcagt acgaatcgcc attggcaata ttctttgata aaaaggcaaa ctcttctcaa
    13381 aaagttccgg tttctgccaa agcacaaaat ggacggcatg ccaccagtgc atttccaaat
    13441 gaaatttccc ataccagctg ttgcaggtca aaccggtctc ctgaggtggc aaacttccga
    13501 cgcattggat acgagtcaaa taacgtgata ggataattct gcgctctaat tctttggccc
    13561 gtgaatcttt acttgccgaa agatctatag ccccgccggt ttcccagaaa ttttgccaaa
    13621 atgatcgcgc cgcggaaaaa gtccgggtaa cacttgcaga ttcctggtca tcttctcttt
    13681 cgaaaaacag acaagtgaat tcaaaatttt taccggattt gattgccaga ttaaacgaat
    13741 gtcggtcggt ttttgtaaac tcagcatctc ctcgccagta aattttgaca cagtattctg
    13801 tggtatctaa ggtcctcttg attaaaactg aattagggct ttctaaaacg attgtggaaa
    13861 aatgtttttc ggggctgtcc caatcggctg gatttttccc ccatctcgac gaagcgtagg
    13921 gaaaattaaa acttattgca acttttcctt gattcaacaa tgccgacttg attctgaccg
    13981 caatttgatc atataccgga tggcaggcgg tctctacttc catcatagta ttcaagactt
    14041 caaaggaact gtaaattatc ccctcccaga tattttcggt ttgcttgatg ttgcggattt
    14101 catttatcct aatcggcaag ccatcctgat ttttaaattt gaaaccaatt tgtcctaagt
    14161 gaaggcgatg cggatttgag cgtaaatatt ctcctgcttc tgaatcttgg tgcgaggcat
    14221 aggctacttt ccgaccgtta acttcatatt cttcataaac ttgatccagc gaatattggt
    14281 ttaaattagg aaaagaatgc caaccccact gcgcctgagt cactaacggg atggtctctt
    14341 cataacattc tggaaaactt tgtaatccgg taaaatcggt agaaaaggca aattcaccgt
    14401 ttcctaccgt aaatggattc agaggatcgc ttgcgttcaa agtgggacta tgacgtttga
    14461 ccaacgcctc gcggtcaatt ttcgcattat tttcacagga aaaaaatacg acgaaagaac
    14521 ttaagaaaac gacaattgcc aataattcgt tcaggtttcg ttttttatcg tatttttgtt
    14581 tattttttcc cattacaagt gcattcaaaa acaagaggtt gtcttaaaag ccccattgtg
    14641 ttttcccctt tttggtcatt gctatcttaa cgaccccgcg cctgcttagc caaaatctct
    14701 actgcgtcaa aaatccagga tacaatttcc acaaaggcgt catgaaacac aaagtaaaaa
    14761 aactaataag acaacctctt attattcgaa tcacaattta tttaagcaaa accatcttgc
    14821 tcgtattgat taaatcaccc gcgaccattc gaacgatata aattccactc ggaaattcgg
    14881 cggcattcca attaacggta tagacacctt cattcaatct ctgcgctacc aatcgagcga
    14941 cttcttgtcc taaggtatta tggattttca aggtaacgaa agccgtcttg ggaacagaaa
    15001 acttaatcgt agtgcttgga ttaaatggat tcggataatt agtcagaaga ttaaaagact
    15061 cagccacagg cggttgacct ttaatacctg cagttggatc tttcgtgtac cattcttcgg
    15121 cattgggatt cactaccacg gtatcctgag gacgtggtcc aacagcttcc aacttcaata
    15181 catgattatc cataaatgtt gccgtgccac aaagcgccgg ataagaatga gctccatcgg
    15241 tatgtccagc tgaccactga atcatatgct cacgagtagt cccatcatta tcaccaatat
    15301 tgatcgccat cccaaactta ttccctaccg tgcgctctac cataaaccga ttgtcttctg
    15361 gctcattgat gtaatcaaac ggcacccgaa attcgagaac ataaccgtca tcggtcaatc
    15421 tgcgggcata atcagattcc gaaacaacat aatcggtacc ttcaacatct tccagatgac
    15481 cagaaccatt caaattatca actccggttg gctcctcggc attgtcagca cccttagcgg
    15541 ttatccggca atttgatgtc ttcgtattag taccgctgtt tggatcagga tcaaatttta
    15601 actccaaaca gtcattctgg aacctctctg tattggtagc cgctacgata tcatctttta
    15661 cctcagcata acaatagaga taatcggcat cgtaagaaaa ccagacttta gccgagagat
    15721 cagcattgtc agtcggtggc gtcccaatat cccgcagata gcataaataa ggcataaaca
    15781 ccaatccgtt cgacggaccg gttagttctg aataaaatgc gtccttctca gcatcaatcg
    15841 caactgcgaa attaggatca atataccaac cggcattagg attcactact acggtatcct
    15901 gaggacgtgg tccaacagct tccaacttca atacatgatt atccataaat gttgccgtgc
    15961 cacaaagcgc cggataagaa tgagctccat cggtatgtcc agctgaccac tgaatcatat
    16021 gctcacgagt agtcccatca ttatcaccaa tattgatcgc catcccaaac ttattcccta
    16081 ccgtgcgctc taccataaac cgattatctt ccggctcatt gatgtaatca aacgacaccc
    16141 gaaattcaag aacatagcca tcatcagtca atctacgggc ataatccgat tccgaaacaa
    16201 cataatcggt accttcaaca tcttccagat gaccagaacc gttcaaatta tcaactccag
    16261 ttggttcctc agcattatca gcacctttag cggttatccg acaattcgcc gtcttcgtat
    16321 tggtaccact gttcggatca ggatcaaatt ttaactccaa acagtcattc tggaacctct
    16381 ctgtattggt agccgctacg atatcatctt ttacctcagc ataacaatag agataatcgg
    16441 catcgtaaga aaaccagact ttagccgaga gatcagcatt gtcagtcggt ggcgtcccaa
    16501 tatcccgcag atagcataaa taaggcataa acaccaatcc gttcgacgga ccggtcaatg
    16561 catcataaaa tgcatccttc tcagcatcaa tcgtaatgga aaaattctga gcatttacgg
    16621 tcaatgctaa caaaacaaga agcaaaaccg aaaacagtaa ctttttcttc atgtcctttc
    16681 tcctttttaa aagtttacat acactctacg acttaacttc acgtaatata gaaataacct
    16741 tttatttttt taatttaaac acatggggta ggctgatttt gaaactagtt gttcccaaac
    16801 acaagcgtga aaccaaatta cctacttcaa tgcatttata atttttcttc atcatttgta
    16861 gaaaaatttt gcacttttac ctttaataat acatagattt tatgagtttt tgtttgtact
    16921 ttttggcact tgaaaattga ggaattttaa atgatcagaa ccaaatactc tattttcgat
    16981 tatctcataa tctccgaaaa tgataaaaat tggggattat atgttactgg cagtggcaat
    17041 gccgacattc cacctcatac tccctatccc ccgactaaac atcccaaagg atatatgttc
    17101 gactggcaaa acggacgaat cctgactgaa ttccagattc tatacattac ccggggaagc
    17161 ggtatttttg aatcaaaagc caccggccgc aagaaaatcg aggaaggcta tattattcac
    17221 ttatttcccg gcgtctggca tcgttatatg cccgacccaa aaaccggctg gaaagaacat
    17281 tggatctcat tcaacggttc aattcctaat gatttgctta acaacggtat tctctcacga
    17341 gaaaaagccg ttttagaaat tggactcaat gaaaaattaa ttaacctcta ccaggaaatc
    17401 ctcgaactaa tcgaaagtca aaaaatcgga taccaggaaa tcatcgcctc cctcactttc
    17461 cagatcgtag ccaatattca ggcaatcgaa aaatcaaaaa aattcttcgg cattgaattc
    17521 gaaactacca ttaacaaagc taaaatcttt atggctgatc gaattgataa aaacattaat
    17581 atggaaaaat tggcatcgga actcggagta ggttattcct ggtttagacg tatgtttcgg
    17641 cactataccg gtttaccacc ggtacaatat tttctcgaat tgaaattgaa taaagctaag
    17701 gaattattag ttaatacttc tttgccaatc aaggaaattg ccgtgattac cggcttcgag
    17761 tctcagttct acttctctaa attctttaaa aatagagttg gtatgtcacc tataaaatat
    17821 cgcgaatttt caagaggaaa ataacctgtg ccgaatatgt gtttcgatca cttgaattat
    17881 cacagtcatt ttttaatttt cccatacaaa ctaaattaat cttaaagtaa tgaaatttta
    17941 aatcaaaaat agtgaaaata tttaaagcat ataccatgcg aatcgtttct gaaaaattaa
    18001 tctcaaaatc aatggcaagt gcaatattct aaattattaa agtctaataa attgaattta
    18061 aatgtaaaat tagtaacagg agtaaacttg tatgcgtagg tttatggcag ttctacttct
    18121 cggtagtaac attttattcg cccaaatgcc ggctgacaca atcaatccac ccgactattt
    18181 aaattctaat ctgccggtat cagtcagagt cgatgaccta atcgctcgta tgactttaga
    18241 ggaaaaagtt tcgcagatgg tcaataacgc cagtgccatt ccgcgacttc aaattcccga
    18301 atataattgg tggaatgaat gtctgcacgg agttgcccta tcgggaattg cgactgtttt
    18361 tcctcaggca atcgctctcg cagccacctg gaacactgat ttaatgttcg agatagccga
    18421 cgttatttca accgaagctc gcgcaaagta tcacgattat ataagcaaag acgaacgaga
    18481 tattcataag ggactgactt tttggagccc caacatcaac attttccgtg atccacgctg
    18541 gggacgtggt caggaaactt acggtgaaga tccttatttg accgctcgtt ttggggttga
    18601 attcgtcaga ggtttacagg gaaatgatcc aaagtatttc aaagtaattt ccactcctaa
    18661 acattatgca gttcacagcg ggccagaacc cgaccggcat actttcaacg ccattgttga
    18721 gaatcgtgat ttatatgata cttatctacc ggcttttgaa gcttgtgtga aggaagcagg
    18781 agctttttct gtgatgtgcg cttataatcg tctctatgga gaagcttgtt gtggttcacc
    18841 tcttttatta tcaaagattt tacgcgagga ttggggattt aaaggctatg ttgtctcgga
    18901 ttgtggggca attttggata tttaccagaa tcacaagata gttgctactg cccccgaggc
    18961 ggccgcgctg gccgtcaaag ccggaaccga tttaaattgt ggtcaggttt attcctctgc
    19021 tattatcgaa gcggtgcaaa atggtttact aagtgaacaa gagatagaca cggcggtcgc
    19081 tcggcttttc accgccaggt ttaaacttgg ccaatttgat ccacccgaat tggttcccta
    19141 ctcggatatt cccatcgaag aaaatgactc cgaagaacac cgacagttgt ctatccgggc
    19201 ggcacaggaa tcaatcgttt tattgaagaa tagcgatcga attcttcctt taaaaaaaga
    19261 ccttaaaaga attgctgtaa tcggaccgac agcggactcc taccaaatgt tactgggcaa
    19321 ttataatggt actccctccc gatacgtaac tcccttgcaa gggattagaa ataaggtttt
    19381 tgagccgacg gctgtaattt atgagcccgg ttgcaatctg gttgaacctg gtaattttgt
    19441 ccataattta tccgccaatg ttttgagtac tgaaggtcaa ccgggattga aagtagaata
    19501 ttttgctagt caagacccaa gcgggcaacc atttttttcc agagttgata aaattcatgg
    19561 tttgaactgg agctacggca cgaccatacc ctcatttaaa ggtcgagaaa atattgcctc
    19621 gatctgctgg agcggtatgt ttaaagcacc cacctccggc cgttacaaat tcacgataaa
    19681 aagcgatagc cgagtgcgct tgataatcga caataaaata atcatcgaag actggaaaga
    19741 gcggaaattc gccagcctga gtaattccat caaattaaaa gaaggtgaaa tgtatcctct
    19801 tgttctggaa ttttcacgct gtggtaatcg cccgcaactc ttcgttgaat gggagttact
    19861 gaaccacaat tcctttaaaa atgctcttga tttagctcaa aagtctgatg tagtgatttt
    19921 cgttggaggg ctaactgctc aattagaagg tgaagaaatg gcggtcagct atgacggctt
    19981 ttataaaggc gataggctga gtctggatct acccaaagtc caggaaaaat tattaaaaga
    20041 actgcatgcc actggaaagc cgatagtact ggtattgacc agcggcagtg ccttgtccgt
    20101 aaattgggcg caggaaaatc tacctgccat tatccagctt tggtatcccg gccaggaagg
    20161 cggaacggct ctggctgatg tattatttgg tgattataat ccggccggca gattgccaat
    20221 caccttctac aaatctgtgg agcaattacc gccttttgag gactataaca tgcgcgggcg
    20281 cacgtatcgc tattttgagg gtgaaccgct atttccattt ggatatggat tgagctacac
    20341 caatttcgct tatagtaatc tcattattga tgaagaaata aaagccggtg agatcattaa
    20401 agttgctgta gatgtcagca accgtggtaa aatagctggg gatgaagtcg tgcaattgta
    20461 tgtgaaaaac ctggatacta ccgtaccaac tcccattcac tccttgcaag ggtttaaacg
    20521 cataaattta aagccgggcg agactcgccg agtggaattt ctcctacagc ccagacaact
    20581 ggcggttatt aaagatcagg atatgctcaa tagtgaaaat gagtcatctc gcaaggttca
    20641 gtatatcgtt gaacccggcg cttatgaaat tagtgtgggc ggaatgcaac ccgtcactat
    20701 ttccgaaacc actcaaataa tatcaaagaa aattaaaatc attggagaat catatctgat
    20761 caaataaatg cgtgcaattt attgatttac taaaacatgc ggaatttagt ccatgaattt
    20821 agctttgacg ttcatgatac tgaaacaaag gtggggtaaa ttagtttata tttaacattt
    20881 aaattggttg gtcattcgaa aaatatttca ctattaacag aaagataaat gatgaatttt
    20941 caaaataata acgacccttc tcatggtttc tatgatttaa aattattact aattatctta
    21001 ctatttttcg ttcttttcat tagcggcttt tctcaaaaca agcctttaga taattcccaa
    21061 atagtcgctc aaattggtaa tatgacaatc accgtagaag attttctcca gcgcagtgag
    21121 ttaaccgtcc gcccggttta ccttaaagat aagaatacag ctattaataa cttgatagcg
    21181 gaaaaaactc tggcgctcga ggctgagggc tccgctacgg agttcttgaa taaaccgggg
    21241 attgccggaa aattacgagg cataaaagaa caaatgatgc gcgaggcgct ctattatgaa
    21301 gtggctttta ataaagcaca agttgatacc actgaaatta acaaagttta ccgctcatct
    21361 atgcgggagt acgaacttga attctacgtg ctcaaaagca aagatttagc ccaaaaagtt
    21421 caaacggttc tggattcaat gccggagttg accgataaaa tgttcgaaga atttgcggga
    21481 gcaattgaca aaaagcccgt ccatcgaatc aattacaatg ataacgagga cgacgtcatt
    21541 cataaagcat tgtactcgga acttttagac accggcacag tgcttggtcc tcttcggcta
    21601 aatgacggca attatattat catgaaagta actgattaca ttgattatcc cctcatcagc
    21661 agtgaggatc aacgcttacg atggaataag gtacagaata agttacatca agccgcggct
    21721 tccaaattgt ggcaagaatt tcaagccgat ctaatgcatg ggaaaaaagt cgagttcgat
    21781 ccaaactcat ttgaagtcct gaagaatctc gcttttgaga tgtatctgtc ttctgacgca
    21841 actgattcta ttaattttca aatagcggaa atagtcccgt ctaagccgga gatcaatcct
    21901 aatgctcctt tttttagaat tgatgaccag acctggacta ttgatgattt caaacgggag
    21961 ttactggctc atccattgat attccgcacc agaaacctga ctaaagacaa cttcgccgag
    22021 caatttaagt tagcagttgt tgatttgatt cgggattatt atttgaccca ggagtcatac
    22081 aaacgaggat tggatcaatc tcctgaaata gatcgaactc tggctatctg gcaggatgcg
    22141 ctaatcgctc aaaatcaaat caaaacggta ttgaaagaag ccgttagtaa cggaataatc
    22201 aacgatgacc cggaagaatt ggctaaatac cgagaaactt atataaaaaa attacaggcc
    22261 aaatatagtt ccaacatccg gattaatctc aaagtatatg aacaaatatc tttaaataaa
    22321 gtagattttt ttgcctaccg tcccggtatg ccacatccga ctacgattcc agcttttccg
    22381 gttcttatcc aatccggcga cctgacctat gctaaacaat ccatagattc tactaagcac
    22441 aaataaaata attgattagg cataaataaa tagggcaaaa tttatttcca attttgcccc
    22501 attttataaa ctatccagat taatcaggct taaaatgcta gcccaatact gaatatatta
    22561 acagaattaa gtaaaccaaa atccgaataa gaatagtcga aataaaaagc agtcgcacct
    22621 ctcaatttgt agttaattcc cccgccgaaa gtcaatcctt gctcagagtc cttagcaaag
    22681 agttgcttat atccaacccg taacgataac aaattaaaaa gcttatattc cgctcccaga
    22741 ttgatatact cagtatcatc gctgggatgt aaagcatccg ccgataagat cagattgcta
    22801 ttcataaaac cttttaaaac atccattgac acaccaaatc gaaggagtag cggtaaatcg
    22861 aaataatcgg tctgcaatcg ggcattaatc agatcattat tgccacttat actctcacta
    22921 atatcatgct gaacctgcaa atcacgcccg tccaggtgca ttttagtccc gtagttagaa
    22981 atacacatac ctaattttaa accggataac tgggtatcaa ataacgcccc gatatccaga
    23041 gcaaaaccac tggctgagct gtggtacatt cgctcattga ttagtttggt attaaatccg
    23101 atcgaaaaat tatcggttaa tttgcgggca taactcaagc caacggcata acaaccagca
    23161 tcgaaatact caccggatcc atccggatag gcgatggtag tccgttcgat ttcttccata
    23221 gttaaaaatg tcgcatttat tccgatatta ccgagttgcc caagtgactg gactgcccca
    23281 gtataagtaa atccgagatc gccaatccat ttgctgttac aaaaagttgc ttcaaagccg
    23341 tcaattccgg caatgccagc tggattccaa tacattgcgg tagcgtcatc ggctatggaa
    23401 acaaatgcgc ttcccattcc attggcacgt gaacctacgt ctatcatcaa aaatccagcc
    23461 gccgtagtgc caactttggt cactcccgcg aacatttgag atgttgccaa tagcaatccg
    23521 gcaaatagaa aaatcttact tttcatttgt attactccgt ataagagaac atttctatta
    23581 atggtcatta ttttattagt tatttaacta ccacaaattt accggtcttt tcaccgatgc
    23641 cttccgcctg gacgtgatag atatagatgc cataagcaat ttctagatta tcttttgata
    23701 acatatccca gatttccgtt ccgtcatcaa gttgagaatc ccgttttatc gtatcaacca
    23761 gctgtcctct aacattaaaa attttaatag tacacttggc tggcaaatga ataaagtgaa
    23821 gttgtctttc accacgacct gaagaatagg gattttttgg ttcccaggta tttgtaacga
    23881 catacggatt gggaactact ctaattttac ttaagtcgtt tttcgctttt tcgacgtcaa
    23941 tagtatcaat cttactggta aattcaaaaa catcatggga caggaatgga aattttaatc
    24001 gtatggttaa agtgtcacct gttctaggta ccaaagagtc tttagtcgtc gccgtgatgt
    24061 aataaatagc tttccagcta ggtaacgaat caggactcag aaaaattatt tcatcatatt
    24121 tcacagtact ggttaaatca taagaaaaga ttccagaatc agctgtagta gttgcatgtt
    24181 tttcgcgaaa ggcaaacggg atatttttat tttctttaag actaattacc tcgaagttaa
    24241 ccggaattgg ttcatatagc ttactactac tgcctttata gtaaaaaatt ggctttggta
    24301 gagatggatc aggagcatcc cgaaaagcta ttttaaaatc acttgtatat aaatcttctg
    24361 gtttagccct attttcggga cggaatctaa actcaggtat tccgggtgtg ttccatccgg
    24421 aacgatccat atcaacttct aacttctccg gattattggc aaaagataat tgaaatccgt
    24481 ccacaatcgg taagccttcg gcgccacctt ctagcgaatg atcatagatc aatgtatcat
    24541 tagtagtcgt attgaccaaa gtgaaacttt tggtagattt tgtagccgtg acacctgttc
    24601 cactcagaga atctttaaaa gtgatttgat attgatgatt atttttaata ttagtattgt
    24661 catagatggt gtaattaatt gtgccagtag cagtattatt tggtccaggt atgattccgg
    24721 attccatttc cgccggttta tacccagccg atggtgcttc cggtctgaca atcgcaacat
    24781 ttgaaccttt ttccacggtt ccagaagcgg caattgatat gaatttgctg cattctgaag
    24841 ggtcaatctt tagagagtca attatcagcg aaccggaggg ttgttgtgtt ccgtggtcat
    24901 aagaggttac agcataataa taagtataac catttactgc cgtagtatct acccaataat
    24961 gacggaggcc agtatcattt cccaaccaga aatgcactcc ctgagtcgga atatcggcaa
    25021 aaccgtaaat tccattatcc aagtcaaatt gagccagtgg ctcccggaaa attacactcc
    25081 cataaccgtc agtaatcggg gtacaatcgt tccaaccggg atcagtcgaa cgataaattt
    25141 tataaccttc aaaatctttc ccggtgatgg gatcgtcgga atcctcagcg aaggtatccc
    25201 agaacagagt tactttatta tcacctgcta ccgctcgaac ggttgggata ttcggggctt
    25261 tagcaaaatt gtaattacga ttataagcat ctgcaaaatt attcttattg cgtactaaat
    25321 cattgtaatt agtaccacaa ataatcgcaa tagacagtct ttcaacctga cccggcgata
    25381 aggggaaata cccacaacca tacaacagtt ccacattcga accggtcata ttggtggaaa
    25441 aatagccagg cgccatcgct tgccaatatg cttcatcgtc attctggtta atactagtcc
    25501 attcataaag attaaaactc gttaatccca gcatatctgt ttcatcaata tcggttttat
    25561 cgaagtgtgg ttcaccggct gtgggttgtc catcaccttc accaatatct gcaccatcgt
    25621 agtcacggga attgggccca attccatcca cccccagatc atcatagata atatcccagt
    25681 cgttatcatt atcaattcca tcgtcacgcc gttcatcaat caaaggatta ttgagccctt
    25741 cacccgtaaa ataatcaaca tatttataac ctaaataaag agcctgagtg gctccttccg
    25801 gttttctatt ttcgtcaatc agaccattta agttgtcatc aaataaattg gagccggttg
    25861 gatcctcggt taaagtcttg cccgcccaat agcgaaaagt cgcattatta aaagtgatcg
    25921 aaagcgtatc ggtcgaattt ttcccaagcc gggttagagt atctcccaaa gtcgtgacga
    25981 ttctatcata ggtatcataa ttgatgaaaa cgatttgatc gccgagacta agggttttag
    26041 gggcaaataa atcctctgaa atagtcttgc ctgagccgct tattccatca ttatcgttat
    26101 caatgccgtc gtaaggattg ccaggacttt ctaggaaagc acctcccata tatccgactg
    26161 gatccggacc ccagcatgcg ccgccgatca aatcattgtc ccacatatag gctaaatcca
    26221 attcccggtc aaatgcggcg ttatcatctc cggtatcctg ttcattttca ctgcacatcc
    26281 ctatattatt gccaatctta tagccgaaga ccattttatt atgtaaatgc gtaccaatgt
    26341 tttcaatatc aaaaagacaa aatattccat cttcgaccgg agcttttgcc cattgaaatc
    26401 ctcgaacata gattctcaac cccaaacctc gccgatttgg attattggca tccgggaaaa
    26461 aagcaaattc gtcattctca taatcatcgg cgacaaagta actttcttca tcggcattga
    26521 acacattttt cccaaaatat ccattccagc aaccagccca gccatccgga ttataaatac
    26581 catatggatt cttgggatca gctatatccg gccaatatga aggccaggaa tcaggccatt
    26641 cggaaccgcc tttagccatc gcgatccggt cgcctgattg attagcaaac ccgggcaaag
    26701 ggaggaaagt acgccaggaa ccatctggtc gtttatctcc ggttgagccg ccggtattgt
    26761 aacttttatt ctcacttaaa atatgaatta gttgattatt agtatctatt acttccgagc
    26821 cgacgaaaat attgccatcc attaagtaat aatgagaact gttaattggc cattcaccga
    26881 tgatttcatc cggagtccac ggattgatgg ctccataagt cccatcatta taaaaagtag
    26941 tgcggaactg attgcccgca tgtagtccca tccgaaattc gttgtcagtt ccatgcaggt
    27001 atgtcatctg ggcaaataaa cccgaagtaa aaagacatcc tgaaattata aataggataa
    27061 agattttctt attcatttat taatctcctg attctcaatt aaaacctatg gctaaaccga
    27121 cttgaatctg gcgcggcgaa gtgtaccagg ttggttgttt aatataatct tcgaccgtgc
    27181 ttacccggtc cggactatat acaactttct tgggattgat agtagtagta taatcggctg
    27241 ttccagtatc ggtgtaaaca tttaactcat ctctaatatc gagcacatta taaatatttg
    27301 caaatagttc taacctaaac ttagacttga ccattaaaat tttattaagc gttagatcaa
    27361 tatttctttg ggaaggtctg cgagcactat tcgtggttaa tcccgataca gccgcacctc
    27421 ctactgtctc cgaagagggg aaactaggag tataaggtct cccactccaa taacgtccaa
    27481 tgagcgaaaa tgtccagccg gagacatcgt agataatctg ggcattgact gtatgccttt
    27541 gatcccactc cattggcact aacgctaaga ccggctgttc gttatttaaa gccgaattga
    27601 aggcgtcggt gggatcagaa taggttcctt cggctgattg ataggtataa tcagctctaa
    27661 aagaataatt ttgactaaat cgtttttcta ttttgaaggt tactccccga acattggaat
    27721 aatctttatt cacaaattga gagtaagaaa cacctgtttt taccgtctga attattggac
    27781 tagtcccaac ccaatcacgt acatcacgat aaaacaaagt caagtcaatc ccgatatttt
    27841 cgccgaattg ttgttgtaat ccaatttcat acattactgt tttttggggc ttaagatccg
    27901 gattcccaaa tagagccgcc cctgagccag tggtaacctt aaaatcagga ttggtataaa
    27961 gatattggaa ttcagggact tggaaaaagt gaccatagga aatatgaatt acgccccggt
    28021 cagtaatcgg gaatgctaat cctaaacgtg ggctccagga acttttagct tttacctttt
    28081 tatgcataaa agccctccgc tcatccgggg tatattccct aattgttcca ctagccaaaa
    28141 gagaatcaac ccattgatca atagtgccgc ggtaattatt tggcatatca atccagttag
    28201 catatttatt cgaatattta aacggtgaat aaatattagg gtcgatcgga tcagctaata
    28261 taaccgaatt tgcatcaaaa taatcatacc ttattccaac attaagaatt atattatttc
    28321 tttctaactt gtcttgaata taagcggaaa gttctttcgg ttccctgaaa taatcattgc
    28381 gatagactga accgacttcc ggaattgccg gctgaaatgg cgtaagttcc tgaccacttt
    28441 cattagttgc cgccacaatc tgataactat gtaaagtcag ttcatataac cgaacctccg
    28501 atccaaattt aagttgatta tacttattaa tctggctggt aaaatccagt tttcctaccc
    28561 agtaggctgt tttccgatta gctcgtgtaa catccatccc gctattcata aaactataag
    28621 aagtaggagc agtaatatca tccggatcaa tatatccgac tgagccttct ggatttacaa
    28681 tgtactcgta ctgagcgcct aaatacttta gtgaatcaaa tttaattgca ccccgctcag
    28741 taaaaggatc aattatttct gctataatat ctttacttgt atcctcttta acgtaaacta
    28801 aataatcatt agaggcattg ggattatcaa aaacgtattg tttcgatttc gattcatagc
    28861 ggctgatgcg cagttcatag aagttcgagg ggttgattat atgatttaac gttaatgtat
    28921 gagccattcc ttgattgtga atttggggca aaccatacgg attatactta taatcatggg
    28981 tattaaattg ctggtaattg gctgtcccag taggattaaa ttggtaatcg tttgtattaa
    29041 ttcggaaata agcccgttct tggtaagatt cattccagaa aaaattataa ttaagcttca
    29101 tcgaacgact taaacgggca cttaattttc cttgaaaggt agttctctcc ataggattca
    29161 tcggtaccag tgagctcccg ccaaatgtga ttccattggc ggcatataaa cttttcattt
    29221 gcgaagtaag aactgtactg tcaattggag caccattagg ttgataccat gtacgcccat
    29281 aataataacc ttcatcatag taataacgtc cattaataaa aaaacttagg ttatctttaa
    29341 ataaaggcac tggcccacct aaactgaatt caccattata aataggattg atttttttaa
    29401 gtggattttc ccattctttt gataatatat ttgagcgggt tgaatcatat aaaattcttt
    29461 tgtataaacc gaatacatta tcgtcactta cataatctcc accataaact ttaatatatc
    29521 cagtataatc ctgcccgccc tctttcgtga tgatatttac tatacccgac attgcctgac
    29581 catattcggc attgaaagta ccacttataa cttgtaactc ctgcacggcg gaattttcga
    29641 tgttgatgcc aatccgcccg tcatatacat cggtggctga aatgccatca acccaatacg
    29701 ctacttctcc cgcccgacca cctcggatat gcaatctacc atcagattcg ataacaccgg
    29761 cgtttaatct cagaacgtcc tggatttgct gaaccggcaa attcgaaatt tgatcggcat
    29821 ctatggtaga aagcgaagaa gtcatatctc taagaaccaa tttcctttcg gcagtcacga
    29881 tcaccgattc tcctaaatct aaagccgtct gctttaaagt tgcatccaat cgagtagtaa
    29941 gatcaatact tactcgaaca tcttcaaact gaatctgttg ataaccaatc atactaatta
    30001 ccactgtgta tgtgccaggt ggaacattta aaatggtaaa attaccctcg ctatcacttg
    30061 ccgctcccat agtagtgcct ttaagtataa cgtttgcacc gataatcgga gccgaagtac
    30121 tggcatcggt tatcactccg gttattttac cagtagtccc gccaaataat ataccattaa
    30181 gtaaaatact aattaggagt aaccatatcc caaatattac tctgtctttc atcacagccg
    30241 cctttatttt tagagtgagt aaaattgtct tcataaaata attcactata actaaaatat
    30301 taaattccag agcaaattaa tagcaaatat ttaatttaaa aagagataga cactgaactc
    30361 cgaaacttca attgatatat ttattgtcat catcattgct tatacaattt ttcttcatca
    30421 tttgtgaaaa aattttacat ttttagtctt aataatacat agattttatg agtttttgtt
    30481 tgtacttttt ggcacttaac ctcgaggtag gttattccta gtttagatgt atgttttggc
    30541 actataccgg tttaccaccg gtacaatact ttgcctagta gatcgcgcaa attctcagta
    30601 taaagacggt aaccctttga ttataacacc gtctcaagca agtaattctc tgaagactaa
    30661 tcttatttgg taagtgcaac ggtattggtt cttgacctca gaccatcgaa cgatgcaggc
    30721 gatgttttag cgaacggatt tgctcgcgaa cgcgtattaa tttcgcacga tcatgattgg
    30781 cgatagcttt atcacgctct ttctttagtt cgcgaatttt acttttgatc tcagtcttat
    30841 tttgcacgac aatctgatgg tgctgatgtt gatcaatttg tagagcctgg cagagagcgg
    30901 caagtaagtg ctctttattg agttgggtat agcctttgac agcttcacta tccagctggg
    30961 aggcaatttc ccgcaactgg gcaacagttt tggttttaag gtcttggtag gtataagtca
    31021 ttgaggactc ctttgagtaa aattaaacat tgcgttaata atctttctca tctccttata
    31081 ttcttcgtat aatataaaaa tttcaagcct tttcgcaagt gcgaattctt tgtgaaggaa
    31141 atttgttgaa gtcgcttttc cttatttcag gatcatgcac ttcctagtag ccgtgaattc
    31201 cggacaggta atttggtaca gatagatacc tgacggcaga ttagccggat gccagaatat
    31261 tttgtagttt ccagccgatt gccgtcctcg gacaagtgtc tcgactaatt gtcctgccag
    31321 attatagaca tcaatcttta tgttcgtagt ttttggcagg gaaaattcga tcacggtgct
    31381 agcgttaaag ggattaggat agttagcttt cagagcaaat tctgtcggaa ttgaagcatt
    31441 tctttcgact tcgatctcgc tcaacacatt attgaataga gaaacgctgg ctaatccttc
    31501 ggttgaagcc gtgaagacaa cctctcccga cttaccttgt tccggaagat agttaaaaat
    31561 ggcgacgcca ttgccggtag tcacttgagg attgctaata attccaggtc cttcgagagt
    31621 tagcgaaatt tgaaaagaat cggcgcaaag gcgattctcc gtatcactga catagcatat
    31681 tatcagataa ttggttacag ggaacgaggt tataatcggt ttatcaataa aaattttcaa
    31741 gctcgccggt tgagtcccat cttcggtttt cgtcaaccgt aaggcaatat ctttgcacgg
    31801 agtcggcatc tcaggaattt cagtatagat catattacca gccgatacac aattaccaac
    31861 aatgacgcta tcgccggccc aggtatcata ccagatagct tgatatgttc cggttttcaa
    31921 gccactaatc cgtgtaaaac gaccggaaat cgcattatta ttatatgatc gaaaccagcc
    31981 aaagccgagg gaatccgccg ccatcataaa aacatccgcg gtatcggcac ttgcattact
    32041 tgaaatcagg ttcaagtcag catagtcaat gtcagaagca actctcgcga aggctttcat
    32101 ctgggcaaaa acctcgtcag tcattagctc tcgatcattc atttcccacc agatgggcgt
    32161 ggccgccaaa ccactcgccc aggctgacca gagcgcatta tgataaattt cgacatattt
    32221 actggaaccc ttttcaatat ttgcggcata catattggtc ggttccgcac cggcttcacc
    32281 gaaaattccc ggtttctcga aaccggtcca taattgacgg gcgacattgc gatatgtcca
    32341 ataactactt cggattggat cgctggcata aggtgcactc caaccctttt cgtaaaggtg
    32401 aacattcggc aaatcacaaa ttcgataacc ttcaggccag taattcgcgg aacttccgct
    32461 cttggagata gtcgtaggtc gtttaaaata atcattttcc ttcagataat cgtggacttt
    32521 ctgaacccat tcgagcgctc cggttttatt accacgcgac catccgtcag tgccgtcaat
    32581 ctcattcacc aattcccaga taccgagact gcgcgagtaa ccccagcgag caagcatata
    32641 gcgatacaat ttctgctgat atacccaggc gagcgaatcg gtataaaaat tagcggcgtt
    32701 acagacagtt ttataggcat tatcatccca acccttataa ccccaggtgc tttgatcgag
    32761 aatatcgtgc ggccagatgg caagcatcat ttctaaatta cgctgctccg cccaatctaa
    32821 aatttcatca attcgagcgc atttacgttg atcataataa ccgataccac tttgtattga
    32881 ttcgatttga taaattccac cgccattacc ggctccatcg taattgccgt tccagtaacc
    32941 gaaaaggttg ccgcccgagg cggctaaaat tcccagccca tcgtcatcat tgcgcacgcc
    33001 ccagggatag taagcgccaa ttccatagaa cggtgtgcca tcgtgataaa tcagataatg
    33061 cggattaacc gaagatacat ttaaacagcc gtgataatcg gattcgacaa cggtaaaatt
    33121 acatttagca ctttcgccga cgccatcaat atcgcgagca aatacctgat attcccattg
    33181 accgataacc agcggagcaa agcgaatttt ccattcgcct atatccatat aattatcgaa
    33241 aaatccatta atccgaatag tatcacttag cggggaaatg aagttcgcaa aaacatcaat
    33301 ttcatcggaa tcgtatggat tgccgtaaga agcctcggtc agttttagag taatttcgaa
    33361 tttatcatac tttccgactg tactcgaatt gacatcggcg atttcgacca ccggaattgc
    33421 tccgatcgta taggttcggc aagccatttc cattgaaacc gcatgttgtt tgcccatcgt
    33481 atcaaccgcc gcaactttat agtaataagt accgatacct atttgcgtat cgcgccagtt
    33541 tttcccttcc ggttcgccta ttaaattttc agtcgtacag gaaaagcctt tgagagtatc
    33601 gcgatacaca ttataatgat caacagctgc ttcattatca caactccaga ctaaatcaac
    33661 atactggttg atcggatcga atttggcatt tagtccttgg atactgattg aaaatccggc
    33721 gataataaaa ttaccaaaac agactgttcc ctgcttatca actttcgtcc cgccgtcaaa
    33781 atggaacacg acctgattgc aataaccacc ggcataatta ctcagcgtaa aagtataagt
    33841 ttgccacgta ttcgaacccg gaacggtagt tgactggtaa ttatctttgc cgttcgtgta
    33901 aataggttta atcgcaaagt taatcgtgat atcggatttg atctcgataa cgattttcgg
    33961 attatgagcg acgttgatga cttccggcgg cgtaaaataa aatgccggcc agtcgctatc
    34021 agttgatttc cggtcataag ccacctcgag aactccctcg ctctctgtca gattgaaaat
    34081 acccggatac cagtcgctat gccagagagt cattagagaa tcattccaca atgtatccag
    34141 cgaaccatct tcaaaatcat ccgtaaaacc ggggatctgt ccaaatagcg atatcgttga
    34201 tagcattata atcaaaatgg atttcttcat gataataccc tccaagattc cccgattaaa
    34261 acttaatgtt catagaacag acaatcattt tttaaagtta tcaatgatat tccgattgtg
    34321 attgattagt gccactcttt gttcgacaca tttcgccgat ctaagtccgt cttccggagt
    34381 atttttcaca taatccagaa gcctttcaat cgtggtcgtc acaacgtgag ttcgggtatc
    34441 cgatgatccg taataaatga atatgcgccc gttcggatcg gctatccatc cgttactaaa
    34501 aactacattg gagacatctc cgatgagctc ctctccttcc ggagccagaa aataaccacc
    34561 tggtttatgg attatccgcc agggttcatt caaatccgtc agaaacatat acaagacata
    34621 acgcaaaccg gccgcggtat tgcggactcc atgtgccaga tgcaaccaac cttcggcagt
    34681 tttgagcggc gtcggtccct cgccattttt cacttcgttg atcgtatgat aaactcgact
    34741 gtcaataatg atttcctcgt caataactgc tttttcgata ttatcagcca gtccccaggc
    34801 aatcccgccg cctgttcccg ccagaataaa atcatcctgc gggcgcgtat aaaacgcata
    34861 cttgccatcg aaaaattccg gatgcagaac cacatttctc tgctgtctcg acttcgtttg
    34921 caaatccgga agccgttccc attttttcaa atcatgagtg cgagcaattc cggcttgcgc
    34981 caccgccgta gaagtatcat actccggaac gttgggatcc ttgcgctccg tacagaacac
    35041 accataaatc cagccgtcag cgtgatgggt cagccgcata tcatagacat tcgtgtccgg
    35101 aatttcggtt tcgggcataa ttatcggata ttcccagaat ttaaaatggt caacgccgtt
    35161 cggactctcg gcaactgcga aaaacgactt gcggtcgagc ccctccaccc gcgctaccag
    35221 acaatattta tcgttgaact tgattgcgcc gcaattaaaa acagcattga ctcctaaacg
    35281 ctccattaga aatggattag ttgcataatt cagatcgaat cgccagttaa tcggaatatg
    35341 tttggcggtt aggaccggat tccgatacct ttgataaatc ccattttgcg atggacacgc
    35401 ctgattagat agattgagcc aagcgtcttg atcggttagc aatttattta aactgttctt
    35461 gaattcattt ttactaggtt tcttcaattc gttattctct tcgattaatg agttgagcag
    35521 tgcgttttag gacttccaag caagcacggg tattatggta acagcatttc cattcgctga
    35581 ccttaggctt agcggggttc ggaatccctt cggcggaaat ttcataatac cattcgcctc
    35641 gttgccaatc cacaaaatat ttatcaatga aatcccagag gcggccggct ctatccaaat
    35701 aggatgaatc accatagagc tggtaggcat taatcagacc gacaagcgct tccgcttgtt
    35761 gccaccactc aaagttattg gctgataaac ttccggccat atcattagcc acaggtagag
    35821 cattatgctg attaaagcct tcggcaatta ccgccgctac aattttatgg atggctgatt
    35881 taacccgcgc ctgaatttta tcatcaccaa ggacttcggc ggattctgta attaaccagc
    35941 tggcttcgat atcatgccca aatgaaacgc cgttcaagcg ggggttccat ttttcatcga
    36001 aaaataaata taaatgatta cttaccggac tgagaatgta tctcaggtgg cattctacca
    36061 attcctgcag acgagcccgc actaaactat cggaactgac ccgcaggaga ttagtcaaag
    36121 cttccagtag gtgaaggtga gaattcattg attttttttc gtttaaatca accgcactta
    36181 accgcagatc ctgggtctcc gaccagtcac gattagcggc ttcataataa cctccattaa
    36241 tcgcatcatg acagtatttt tcaatcaact gaaaaatctg gtgcgccatt tcaagagccg
    36301 ctttttcacc ggaagccgca taatattcac tgagaccata aatggcaaaa gcctgtccat
    36361 aaattttttt cttatcttca aggacttcgc caccaggcgc cactaaccag taaactccac
    36421 catatttact atcgctgaaa tatttagtca gataattgta tgcccggtta gccatttcaa
    36481 gatgccggct atccttatca aatctaaaaa gcgccgagaa agtccataat atccgagcaa
    36541 tcagcaccac actgcgcggt gcggttttat ccacctgtaa atcgttggta atccgaccat
    36601 aaaatccacc attccgacga tcaattgaat atgtcatcca gaacggaatt atattttcgt
    36661 ataaattgcg actaacctgc tgacgccaat tcgcaattgc atcatttttc atattctatc
    36721 tcgatttgcc aattataaag atttcttcaa aattaatcat cttccatttt atcgtaccaa
    36781 tttttcttca aaaagatgga ggttactata attatcaata aacaaatccc ggcattacca
    36841 aactttttca gcacaaaata aattggcaaa gccactaagg tcgtttgcca gataattccg
    36901 atcagtacat tcagtaaatc ccgcttccag gaacttcgat tgacaaagtt gggatcttca
    36961 gctaccactt tctcatgaat gggtttccag aaaccccagg gacgcactgt tttataaaat
    37021 tttttcaata ccgcctcatc tgttggtgga tgacggtagg ttccaatcag cgcccctgcc
    37081 agtgagatta ataaaatcag tggaaaataa tacaaatcca gcgtgtcgaa aatctgagga
    37141 aaaattaacg ccggaataat cccagccacc attccccaga aataaccata gccgttgaat
    37201 cgccaccagt accacttcaa aacattggaa acgacataac tcccccataa agccgaaaca
    37261 atccattgca aaatactatt aatatccaga gcaaacattc caaaaattat actaatgacg
    37321 acgataccaa ttcccgatat ataagtcacc gatgacacct ggcggttggt tgctttggga
    37381 cgcatatatt tcaggtaaat gtcgtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37501 nnnnngatga cacctggcgg ttggttgctt tgggacgcat atatttcagg taaatgtcgt
    37561 tgacaatgta agcctgcgcg gcgttcagcg ttccagcaaa agtaccaata aacgccgcca
    37621 atagtcccgc cagcatcaga ccagtaaaac caatcggaac aaattcactg atcgccgaag
    37681 gtaaaatttg ctcgaaatcc agacgtccac cgacaattaa atccaaacga tcgtaataaa
    37741 taatgcccaa cacggcaaat ccagtaatca ttatataacg aatcggattc agaatcaccg
    37801 aaacaaaacc gctcatcatt ccagcttctt tgggcgagcg agtcgccaaa attttctgca
    37861 tatcgtaatt cggcgccggg ccagccgcac tgactaaaat acctttgaac agcatcatca
    37921 taaagaaaat cataaaaagc gagtagccgt cgctggcgat cttatcattg acttcggcaa
    37981 taatgccact ccaatccata ttcaattccc aaccgaaaaa gggactcatc cagccttccg
    38041 gtactatcag gggatggatc gaaagcgccc gcatagcaat tattccaatt acgattgcag
    38101 agactcccat aatacaatac tgaatcacat ctgcccagac gatactgacc attccaccca
    38161 gaatggtata aaaaacactg aacgtcgtaa aaacaatccc gtacaaatgg gggacaaatt
    38221 tgtctggaac gctaaaaggc acataattgg atactacttc ccacgggata aaaatttcca
    38281 taaattttcc caaaccgata aagccgtagg ctaaaaatcc gagacaactc agtaaagcga
    38341 aaactactac gatattatgg gaaagggtcg catcccggcc agaaccaaaa cgcgttccaa
    38401 tccattctgc gcctgtcgtg acatttgagc gccttagcca ggctgacaaa tataccatta
    38461 aaaatatctg attaaaaacc ggccagagcc agggaatcca gattgattta aacccataaa
    38521 cgaacatcag ggtcaccagc cacattgtcc cggaaatatc aaacataccg gatgcattcg
    38581 atattccaag catataccaa ggcaatttat tgccgcctaa taaatacgcg gttttatttt
    38641 cctgtgcccg ctttttaaga accaggccaa tgacaacggt aaaggataag tatagcagaa
    38701 ttataacgat atcaattaat tttaatcgca taaaaattcc cgattttcat tatactgttt
    38761 cacatactgg taaattttgg cattctattc gacagtttta tccagaggcc gaccaagact
    38821 tttatataaa tccttgcgac cggctttatc tttcaccaaa atttctcgcg catatttggc
    38881 aacttgctcg gcgacattac gagggactac aatcacaccg tcaccatctg ccactactac
    38941 gtcgcccgga caaaccaaaa ctccgccaat gacaaccgga cgattaaccg attccagctc
    39001 attacgccct ggtctaatgc cgcgaccctt ttttcttaaa tataaaggca ctttctcctt
    39061 ggcaatttca tcagtatcgc gcgcacaggc atcggtgaca actccgaccg ccccactttt
    39121 atgccaagct aagatattgt aagagcctat ggtgccaata tccttttcct caacatcgtc
    39181 gagcacaatt accgagccgg gtcgaatcaa ctcggcaaaa gtttcaggcg aataaacatt
    39241 ataaaaattc ccttcccatt cctgaaattt ttcaccggct tgcggtcggt caggacgttg
    39301 cgtgggaaca tagcgcgccg tgatggcaat gccgcgaatt tgatgcgata ggtcttccag
    39361 atcgagccag tcggaatgaa tcgccggatc aaccaatccg acgcccggca agcccaccat
    39421 atccattcca tcggaaacat cagccacgcg caatccttcg aagagtttta ggatgcgggc
    39481 gtcttcttcc ggcgcgtaag ttttcgttgc gataaaattt gccccaattg acaaatctac
    39541 tttatttttt tcttgagaaa aagccaagga tacaataatt atcaaaacaa tataaaatcg
    39601 aaataatttc ttcataatta atccatcctt aagttacttt aatagttcat gttcatcttt
    39661 taacatctga atacagttta ttaaagcgcg ggtttcatga taattgactt tccagtcgta
    39721 tcctttagga cccttggcgc gctcgggact tttatccaga ccttttgaat accagccgcc
    39781 gtgctggtga tcaataaggt agcgttggat
//
LOCUS       DAOSMP010000017        37516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_1141_length_37516_cov_20.395852, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000017 DAOSMP010000000
VERSION     DAOSMP010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 37516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37516
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1141_length_37516_cov_20.395852"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2487
                     /locus_tag="PKX09_04470"
     CDS             <1..2487
                     /locus_tag="PKX09_04470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU17109.1"
                     /translation="VGKPLLKVFNIINESTGKTPPNPVKRILKEGLVIELANHTVLIS
                     RNGQKIPIDDSGAPIKDEYGNILGAVLVFRDITEQRKAKKELEFEKTLIDAMLNTFPD
                     YIYFKDLNSRFYRVSKSLATEVFGLPDPKDAIGKSDFDFFPPEFAQKDYNGEQEIIKT
                     GKPIIDRAERKNWANGTVSWSSITKIPLTDKDGQIIGTFGVSKNITESKKLEEALRQS
                     EEKYRKQFEESIDAIFLADAETGILIDCNKAAAKLVGREKSEIIGQHQRILHPPSEII
                     NGQSRTFLEHKKAKEGQTLESQIITKSGEIRDVEIKASVYEIENQKIVYALFHDVTEQ
                     KRIEAELAKERNYLRVLLNTTPDHIYYKDRDSRFLLMSKSQADRFGLSDPKEAIGKTD
                     FDFFTEEHARPAFEDEQRIIRTGQPIVGIEEKETWPDGGISWVSTTKMPLLDEKGEII
                     GTFGISRDITERKIAEEQLLKAMEEVKNSEDKLKNIINGSAIPQFFIDEDHKVVYWNT
                     ALTELTGIKAEEIVGTSNHWKAFYQEQRPCLADLLVEGNIKNLPEWYSDKYQKSHLVE
                     NGFEATEFFPNLGKDGKWLFFTAVAIKNSKGEIIGSLETLEDITESKRAEEALAQERT
                     LLKTVIENIPDKIYAKDTEGRFILCNKAVVERMGKSDEKEIIGKTDFDFIDHKLANQF
                     RANELEIIKTGIPLLNHEEPLEQAEGRMRWNSTTKVPLRDLQGNIIGIVGVGRDITER
                     KQAEEQREKFIAELQEALDKIKTLKGLIPICACCKKIRDDQGYWNSVESYIKDHAEVE
                     FTHGICPDCMKKLYPNYCKDDNDKKKPETK"
     gene            complement(2489..6244)
                     /locus_tag="PKX09_04475"
     CDS             complement(2489..6244)
                     /locus_tag="PKX09_04475"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOU17110.1"
                     /translation="MKATRFFRNTILIILLFGESLFPNISASAQSVEEAWQAWNRNDQ
                     KLVEQKFQEAIQANPNDTRAYIGLAYLYEMQYKYLEAWQTYAKVIETEADFEPYIFAA
                     MMAPRLTNAEEYKSAGIQMLFDNLARTARHSTMRINANEKLGHYYNEHANLKKALFYF
                     NKIDAITDWMLIGPFDNISASGYDKVYPPETEFDTAAVYRGQNDIAIKWFRIPQVRYD
                     KWIDLRRYFADNDAVFYGNTFIFSPQKQTVHLRVGTSGSLKVFLNDELMIECFDENNN
                     GFDTYSVKTELQAGWNRLLIKCGFSEITQCNFAARFTDPAGNSVTDLKISALTHSYKS
                     HPGAVKEPIPNFAEEFFLTKIAEHPDYLENYLLLAECYALNDKAVESELILREALRRA
                     PTNAMIYLHLIEAYLRGEKYDEAKSAYEKIYQLDPDIPGVLEYRISELLAKKNYDEAK
                     NLLDRLAQLIPDSPNLQLQLIAYYSKRGLTEKVIEIANTAYHQFPDNWNIVETKANLE
                     AYVKQNNKAAIPILRKYVKNHTEQTAFLKLADYYKNAADLKNWEKTYRQAIEYYPASP
                     SLFYQMAQNFYNRHNYSTALDYIEKALQICPQCAGYWGTRAEIQRSSSQSAAAIKSYQ
                     RALELSPTDYDARDKLRQLQGRASIFNNFDTVDIDSLFRNCPSSEQYPGEKAVILYNA
                     CRRVVYGGATEMSQEILATVFNLEGIDAFKEYRIPYNAYNEELIVEKAQTLKKDGSKI
                     DADVSWGYIVFKSLEPGDAVYLKWKIRNYYSGKLSQHFWDDFSFNTFFPVREVCYALL
                     IPPNVKFQYKGQFMPDEPSAIKETPDGILYEWRLNDVPAIKEEYNMPPLADIGKILHI
                     SSIPDWDYIVNWYIDLAETRTRSCYEIDEFLKTLLPENEPLTEQEKITRIYNYITENI
                     RYSSVSFRQSGLIPQKARDVFVTRIGDCKDKAALGIAMLRSAGFTANFVLVNARYEGL
                     NKHMLPSIAFNHAIVSVATQEGTLYLDLTAQNFPVGSVPLADQGAFALEIKKGCQPFY
                     LTRHFFMGSVRQRRTKVEVQTDNSIQVELHTTRKGAQTAEFRAIYRFKSQADRDKELI
                     ESLAQDFPNVELISFNLFDLDSIKPILDYEYTFKVPDYLTEAGKFLILRIPWADRLSP
                     IRALSYDKRNYPFTYWPNADTISEEITITFPAGYQPLEFTPYYECSCPIADYRLTLDY
                     NDRILKGTRLMINKESEVTPEIYPAFKEFYNKVVKADGYQILIKRLE"
     gene            complement(6403..6876)
                     /locus_tag="PKX09_04480"
     CDS             complement(6403..6876)
                     /locus_tag="PKX09_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17111.1"
                     /translation="MLKQFKVLLIWGMLIVGFMACNDNGSEPPTAAILSLLRTEYGGC
                     NEYKGGDILPKAAIQNDSLSWEISDDTLKIFTGITYICCAPFDVESEQSGDSLKIMIR
                     DVCGISSEHCYCRCICHYTFLTIFTGYSGDRYHLAVWLHSPRQAQDSLLWQVEIP"
     gene            complement(6941..7384)
                     /locus_tag="PKX09_04485"
     CDS             complement(6941..7384)
                     /locus_tag="PKX09_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17112.1"
                     /translation="MALNFLERFPLLYDWLKAHTTLLKYAGLISLITFFGTILLMGVL
                     ILMIPTDYFSNPKRRKSFLITKRPLLGVILLIVKNLIGLIFLIVGFLLLFIPGQGILT
                     ILVGIILMNFPGKYRLERKLVQRPGVLQGINNIRKKFNRPLLGFE"
     gene            complement(7525..8139)
                     /locus_tag="PKX09_04490"
     CDS             complement(7525..8139)
                     /locus_tag="PKX09_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015106973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HOU17113.1"
                     /translation="MKIIKKYSHLNGEEYLIVHHKDLYNEIKSVIKNINAEGLKTKVS
                     NENRKAGTQLYSPIELNKAFDREFSKRNWNESRYNYYITLNRELMEKSLLMPAGEQKK
                     FLIEHGEKNPIFSYNQTDFVKNKVAVEVQFGKYAFVAYDLFVKHMLFYSGGMINLGIE
                     ILPTKNMQSQMSSGIAYYEGEVYNVMRQGRNSPPVPLLILGIEP"
     gene            complement(8136..9089)
                     /locus_tag="PKX09_04495"
     CDS             complement(8136..9089)
                     /locus_tag="PKX09_04495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOU17114.1"
                     /translation="MEVKETLFNYVNPAIEYQILNGDSCYILQNLPDGKFDLIITSPP
                     YNIGKEYETKTCIEKYLETQEEIIRQLIRVLSNQGSLCWQVGNFVDKGEIFPLDIYYY
                     PIFKKYQLKLRNRIIWHFEHGLHASKRFSGRYETILWFTKTDNYIFNLDAVRIPAKYP
                     GKLHFKGPNKGKPSGNPLGKNPSDIWKIVVEDWDNEIWNVPNVKSNHPEKTEHPCQFP
                     VELVERCVLALTNKSSWVLDPFAGVGSTIIASIKNERNCIGIEKEAHYCKIAQNRIEA
                     LKAGNLKVRPINKPIHKPSKNDKVANVPNEWLKLKFNGYSK"
     gene            complement(9197..9532)
                     /locus_tag="PKX09_04500"
     CDS             complement(9197..9532)
                     /locus_tag="PKX09_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HOU17115.1"
                     /translation="MNTEQLFESLRKLQEPKGYFFNKDRQHVDRLLNSLLINKERYGY
                     MACPCRLAVGDRESDRDIICPCVYREQDVKEYGRCYCGLYVSADWNAGKIPDRYVPER
                     RPLEKILGL"
     gene            complement(9529..9780)
                     /locus_tag="PKX09_04505"
     CDS             complement(9529..9780)
                     /locus_tag="PKX09_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOU17116.1"
                     /translation="MDKKVKLYALSTCIYCQNTKAFLKSLGIEYDSIDVDLLDKEQKL
                     AVIREIHKLTPTVAFPVIVIGDNVIVGYRPEEIKKALGL"
     gene            complement(9846..11402)
                     /locus_tag="PKX09_04510"
     CDS             complement(9846..11402)
                     /locus_tag="PKX09_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOU17117.1"
                     /translation="MSNKTGLEEQFSCFRRNIIGIDYQYNFPGGCRPILYADWAASGR
                     LYRPIEEYITNVLGPYVANTHSDATLTGTIITDAYHQAHKIIKQHVNAGPDDVLLLAG
                     FGMTAVINKFQRLLGLRVPDKFKEKISIPPQERALILLTHMEHHSNQTTWEECCCDTS
                     LICKKSDGLPNLEHLRNILELNRDRKLIIGSFTACSNVTGLITPVNELAEIMHQFGRL
                     CFIDYSASAPYVEINMHPPKPEQKLDAIFFSPHKFLGGPGSSGVIVFDKKLYNNRVPD
                     QPGGGTVLWTNPWGEHRFFQDIEVREDGGTPGFLQAIRAALAILLKEEVGVENILAQE
                     HYLTEILLNELAGIPDINILESQNHNRIGFVSFYSLKVHHNLIVRLLNDRFGIQTRGG
                     CLCAGTYGHVLLHIDDQRSKAIMSQIDQGNLSLKPGWVRISLHPTMTEVEVRYIASAV
                     REIMKNYVEWGKDYHLNPETGDFDHLKQPKASIKLRETFKAALRIFLPVVILNAYICF
                     SFGNQIAISL"
     gene            11559..>11894
                     /locus_tag="PKX09_04515"
     CDS             11559..>11894
                     /locus_tag="PKX09_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17118.1"
                     /translation="MNKHFLIWAIGLWLSACATHPTISPVPLKPGETYTGVTFSVENV
                     APIFVYRRGVSERSDIGFRVGLPVYGSGIDFSYLLFHHDNIADVLNLSFSVNPNSNLD
                     MTYYSVRTFP"
     assembly_gap    11895..11994
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12229..14241
                     /gene="uvrB"
                     /locus_tag="PKX09_04520"
     CDS             12229..14241
                     /gene="uvrB"
                     /locus_tag="PKX09_04520"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOU17119.1"
                     /translation="MNKFKLTTNFTPTGDQPQAIRELTEGILRGDKFQTLLGVTGSGK
                     TFTMAQVIQNVQKPTLVISHNKTLAAQLYGEFKAFFPENAVEYFISFYDYYQPEAYLP
                     VTDTYIEKDSSINDEIDKLRLKATSSLISRRDVIVVASVSCIYGIGSPEEYTRFIVTL
                     KKGDRIGIRQLFGRLVDIYYARNDLVLERGKFRARGDIVEIRPAYEDYAYRIELFGDE
                     IEHIYICDPLTGEVTNEQEMVVIFPAKHFISSEETVQRAIVSIEEELEARLEELRSAG
                     KLLEAQRLGQRTRFDLEMIQEVGYCSGIENYSRHFSGRKPGQQPYTLLDFFPRDFLLI
                     IDESHVTLPQLRAMYNGDRSRKEVLVEYGFRLPSALDNRPLKFHEFEKMVNQAVFVSA
                     TPSDYELEKCIGVVVEQIIRPTGLLDPEIIVKPTKHQIDDLIDEIAAVTARKERVLVT
                     TLTKRMAEDLSEYLRGMGLRVRYLHSEIKALDRVSILRDLRLGNFDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSEKSLMQVSGRAARNINGRVIFYADEITESMRKVIEEV
                     SRRRKVQQDYNIAHNIQPRTIYKSVAEVMQTTAVADVKPTPIQTDQPVGDRYRAKMET
                     SEIIELLRREMLAAAENLEFEKAAELRDEIARLTGQKKSLSRENLL"
     gene            14412..>15289
                     /gene="purD"
                     /locus_tag="PKX09_04525"
     CDS             14412..>15289
                     /gene="purD"
                     /locus_tag="PKX09_04525"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOU17120.1"
                     /translation="MKVLIVGSGGREHALVWKLSQSPNIAKIYCAPGNAGTALLAENI
                     PLKDNNLDGLLKFALENSIDLTVVGPEIPLVNGIVDLFRAQGLKIFGPTSAAARLEGS
                     KVFAKQFMSRHQIPTAAYQVCDSRAAMESATHFKTFPYVIKVDGLAAGKGALIIHNDD
                     DLREAFEAIWDEKRFGTAGNKVVVEDFLLGEELSVFVITDGEHYLILPPAQDHKRIGE
                     NDTGPNTGGMGAYAPAPLGTPQVMHNVEKQIIIPTLNGLKAEGIPYTGVLYCGLMING
                     TQPYVVEFNARFGDPET"
     assembly_gap    15290..15389
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15390..15576
                     /locus_tag="PKX09_04530"
     CDS             <15390..15576
                     /locus_tag="PKX09_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HOU17121.1"
                     /translation="NAHFLTNGGRVLAVTALADNLEKAIASAYERIAEITFEGAYYRR
                     DIGQKGLLRQKDLLEQI"
     gene            15592..16815
                     /locus_tag="PKX09_04535"
     CDS             15592..16815
                     /locus_tag="PKX09_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOU17122.1"
                     /translation="MDNERINKLQQSFGILGESDYIKEIVETIDQVARTDITVLICGE
                     SGTGKEVVANAIYRHSLRKHQPFVKVNCGAIPAGILESELFGHVKGSFTGAIEDRKGY
                     FETANHGTIFLDEIGEMPPETQVKLLRVLELGEFLPVGGSEVRRVDIRVIAATNKELA
                     AEIERGNFRKDLYYRIKTITIYMPPLRKHPEDIPLLVEKFALDFANRNSIAFKGFSPT
                     ALNLLREYSWPGNIRELKNFVESMIVLEKGEVITSSMVEKHLLGEPRHIPSPHLPMKI
                     SKSVDQAERELILQQIMVLRQELHEIYEAVITNPLPLKYEALRELEIPPSLPRPKVEE
                     KEIGKLDSAEIINDEIINPEMVGKVTLAEVEKELIQRTLKKFNYRKRQAANALQISER
                     TLYRKINEYGLEESR"
     gene            16821..>17275
                     /locus_tag="PKX09_04540"
     CDS             16821..>17275
                     /locus_tag="PKX09_04540"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOU17123.1"
                     /translation="MLFKRLAPIIKFTPAVLLCLIINSCWLYSFKGTLPPGIKSIAIP
                     LFEDRTAEFNIQQIVTDKTRLGFIKENLLKVVEEDNAHSVLYGTILSIDDKPLVYDED
                     ENVGESVTEYRVTIKIEIEWYDKVNDRTVFKKQFIGYSEYDPTGATSKTR"
     assembly_gap    17276..17375
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17376..18548
                     /gene="asnS"
                     /locus_tag="PKX09_04545"
     CDS             <17376..18548
                     /gene="asnS"
                     /locus_tag="PKX09_04545"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOU17124.1"
                     /translation="GLIQGIVSRNDVSEKIFNYKEILTQESSVSVSGLIHEDKRAPGG
                     FEIIVKDIQVYQIAKDYPITLKEHGIEFLMDNRHLWLRSRKQWAILRLRHAVYYGITQ
                     YLNENGFIRFDSPILTPNACEGTTTLFEVPYFDLGKAYLSQSGQLYLETGIMSLGRVY
                     DFGPVFRAEKSKTRKHLTEFWMMDAEAAFVEHEENMRIQEELIRFVIRKILAECQPEL
                     EILERNIEQLRKADAPFKKMTHRAAVEYLRSKGSSMNETEDLGAQDETLLTEGSDVPI
                     FVEKWPKEIKAFYMKRDPENPDLVLGDDLIAPEGYGEIIGGSQREDDYDLLLQRMIAE
                     KMPLDEYQWFLDLRKYGSVPHSGFGVGLERLITWIAGVPHIRETIPFPRTIYRLRP"
     gene            18548..19003
                     /locus_tag="PKX09_04550"
     CDS             18548..19003
                     /locus_tag="PKX09_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HOU17125.1"
                     /translation="MTVSPKIIAVLGGRECDEQTYQLAFDVGRAIAERGAILLCGGRT
                     GVMEAACKGAVSAGGLTIGILPGADKNDANPWVKIALPTGIGVARNAIIARACDAAIA
                     IGGKYGTLSEIAYCLQLNKPICGLNSWPEISDLRQFNSASAAVAFAFGE"
     gene            19008..19283
                     /locus_tag="PKX09_04555"
     CDS             19008..19283
                     /locus_tag="PKX09_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOU17126.1"
                     /translation="MAIRVAIIVQGVVQGVGFRWYVRRIAMRYRLTGYVKNLPDGDVY
                     CEVEGEEGMINDFISELKAGPPLSHVTNVIVNKLPDLVGYKSFEIRF"
     gene            19280..20938
                     /locus_tag="PKX09_04560"
     CDS             19280..20938
                     /locus_tag="PKX09_04560"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOU17127.1"
                     /translation="MKQIIKIGLAQINPVLGDLSGNAAKIINFIQEADQQGVNLLIFP
                     ELAITGYLPQDLLLNRSFIDCNLQCLNKITAACRSSMVVILGFVARENDKLYNAAAVI
                     KSGQLIATRYKTLLPNYDVFDERRYFTPANENLPVPIEIGGHSVRLGVEICEDLWDSN
                     SDIKVTDQLVVQGAELLVNLSASPFEYDKRDRRRQLVLDKVIKLRRPFVLVNIVGGRD
                     ELVFDGNSFALDASGTLIGWGGEFKENLAVFELDLVSGQGHRMPWPETQREASIYQAL
                     TLGVHDYFYKTGGRQAIIGLSGGIDSALVACIAVSALGSENVLGVFLPSRYTSAASRE
                     DSVVLAKNLNIRLIEIPIEKIFQSYLQELKPVFGQTSPDVTEENIQSRIRGNLLMALA
                     NKYHAYVLNTGNKTELALGYCTMYGDMCGALAVISDLSKLDVYKVAGYFNETAERLII
                     PERIFTKIPTAELAPGQVDPFDYTIVSPLVDLIINDQKTPAELIQMGYATDLVNDIFE
                     RIKFAEFKRRQAAPGLKITGKAFGLGRRYPIINHFKEKGEKQCE"
     gene            20929..21393
                     /locus_tag="PKX09_04565"
     CDS             20929..21393
                     /locus_tag="PKX09_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17128.1"
                     /translation="MRVVRYWLTLICIGIALHTASPLLAQNPTDIDGTIWRRMKYDNK
                     YYWLLGYLQGLEKSDEIIQLCLAEQEKREFAFTVPFYVDQMRARISAYFPPHNIQDIT
                     QLIELLNTFYKDKYNQKIKIEAALRIVLARQKGQIEQSDFWLNEARRALIQK"
     gene            21419..21955
                     /locus_tag="PKX09_04570"
     CDS             21419..21955
                     /locus_tag="PKX09_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17129.1"
                     /translation="MNDHTEFEEINLARHIYRYLITIGIALFGLFLISIEPNVLRQYR
                     SWIPSQTFNIYYLTWFAVCTISAILCFFWGVLCQFLRGNNCLGNVLWYVGIFTITLFF
                     ATVFFQILKTDRVVGFAVTFGTLIISAMVTMAMSYIFYLLNFNSRYHFWLFTLAIISA
                     TSAYNWLYLSVIGKIPAF"
     gene            21990..24353
                     /locus_tag="PKX09_04575"
     CDS             21990..24353
                     /locus_tag="PKX09_04575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOU17130.1"
                     /translation="MIKYILERMPLVGICVAYLSGIVTDHTLHLLPEVWFIFLLIDFL
                     IGIIFSSKARSVLLIALALFCLGGMNHALRTVTGPDHLSQFYFSNEPDSIIAMVEQAE
                     FSGDGKLKIRLKNLQIKHSDWQKWNGKLILTVKEPENNVIYGDWMAFYTRLNQPATAR
                     NPGEFDYWRYLTNHHIYATAYLDAGQTPTIIGRSRSLRRAANYSRIKVEELIDKSMSG
                     EQNAILKALIVGVRGEISDETNQAFVDTGVVHVLSVSGMHVVYVTMVIMLILGFLRIP
                     KKPKMVLAILGLGFYALMVDLRPSVNRAVIMAILTLIAQGWEKRANVYNTIAAAALIQ
                     SLFDPLQIFDMGFQLSFMAVISIVYVYRQLEYLLPLPLKTSNIKNRRLREIWQLFLVS
                     LSALLGTIPITVFYFQRIPIISLVTNLAVVPLVGLIGGLGFGQVILGFLWSGFNLTFG
                     EVQTLLISLLTQIVKISGRVPIAYLTIPAISLAGVYFGYLILLILLNLDKKRVQKVAL
                     FGFLIGINFLIWTQNLSKPVLQVTFLDVGQGDAIFLEFPNRETMLIDTGDRTFRRDYG
                     ELTIAPFLKRKGFQRIDYLALSHPHNDHIGGAPYLMRNFQIGEIWEPDISASSQIYRE
                     IHTLADSLQIPIRHLYAGDCFPLAESLTVFVLHPSKAFLESHPPGFNDYSNVLKISYR
                     QIDFLFCGDVEKFSESYLNLWHTTLQSEILKVPHHGSSTSSSQTFLEQITPDLAVISV
                     GKNNKFRHPSAGTIARYEALGTEIHRTDLSRALIINSDGHKYWIKPW"
     gene            24448..>24686
                     /locus_tag="PKX09_04580"
     CDS             24448..>24686
                     /locus_tag="PKX09_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17131.1"
                     /translation="MVNNQPENPEILKGYTAHWIVPVSQPPIEKGVIIVQGQQIIEVE
                     SADNLQTNIETPLVDLGDSVIFPGFINAHTHLEHPP"
     assembly_gap    24687..24786
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <24787..25521
                     /locus_tag="PKX09_04585"
     CDS             <24787..25521
                     /locus_tag="PKX09_04585"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU17132.1"
                     /translation="EIIRTFRELIQDQTPEKKITKHLTPSYVWATSPQLLHEIAFSER
                     HIAIHIDMIPEENEFTLNGSGLLRQILLANEDFDYSWQVPGLTAIRYFFANHFYARHN
                     ILTHMNCVTSDEIDFIKEFGVKVNVCLCPRSSKILSQIKTPVDLLLEKGINLCLGTES
                     RVLVPDLDIRKEMVECVDTYGVSPELALKFATLNGAYAIGFHKEVGSLEKGKIARCLV
                     VDCSGLTTTDPYEMILSTPHSPRWLI"
     gene            25748..26890
                     /locus_tag="PKX09_04590"
     CDS             25748..26890
                     /locus_tag="PKX09_04590"
                     /inference="COORDINATES: protein motif:HMM:NF037429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17133.1"
                     /translation="MIREQILKDLIHLPQNKFPVTSAYFTLDNIAGNRKSHIVEIKKQ
                     IRYKKEKTYFRQLSTEEQESVLSDFDRILHWFGKELNPAESISSICFSSSGANLWRTL
                     NFKKPLPQNILVIQPYPYIRPYTILFSRYRNYAVVLVDRSKAKIFESHLGEFREHFHI
                     EDNAPESVKVGGFKGRQERKVERNIHQGVIQHYKEVAQEIFDLHQKFDFHWIILGGRK
                     EVLAEFSKYLHPYISARIAGQIEVEPTAPLAEVLKKVEKTEEQARNNYEQALLKSLLD
                     RKSAGQTVDGIQAVLDKIQDNWVDKLIIQEHFFKKGLFCRNCGFLSLKPLASCPAGCG
                     KLERTNDLVEHLVHRALKQGVDLQFVSSNMDDYAGIAAILRFSIGG"
     gene            27111..28058
                     /locus_tag="PKX09_04595"
     CDS             27111..28058
                     /locus_tag="PKX09_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17134.1"
                     /translation="MKAKNFYVSIILAISLSACGASRAKPEKTGEKTTKAKSEKAENL
                     PESQSSKSTSSGPQPKIATAPVKATAADQSRLINELTFEVKKLSAEVKHLQSALKELQ
                     AKSQMWDNPLSIYDKEIIMANGTSIFGKIIYQDEKMIKVETWIGYLIIDKNQVVRVVT
                     NIVEKPDEQYVPPELASDIKDDKPITIQAPYISKTGEPAPIGEPTSRVPNCVLVGNIK
                     ERKDLSGNTILSGEIKNIGGRRADFVKINIVFRKNWSGDTETRTAFVEGSYVTFEGTG
                     ITSNNSLLPGATGTFEMIIPKDFGQFIGYTYNIDWEVYR"
     gene            28058..28837
                     /locus_tag="PKX09_04600"
     CDS             28058..28837
                     /locus_tag="PKX09_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU17135.1"
                     /translation="MPRSFNLSLLTVVITAVICFGQGTSSSSGTPIHDSNKTIDQKDI
                     EIYNLKEELEYLKKELRKLQEINTSLCDSLESKHTLLETTSEKNVDENYNLRIYHQTI
                     EDDRRLNQEIVKEIQSTAPIAVPFKSPEPSGDPEFRERYNEALNQYFDGNLSDSQKNF
                     KKLLELRQDHPLSDNCQYWLGECLYSQEKYEEAIAAFQTVKALGDGNKADAALFKIGL
                     CYLKLDKKAAASSAFNDLINFYPKSDLVPKAYQYLLSQDKF"
     gene            28839..29564
                     /locus_tag="PKX09_04605"
     CDS             28839..29564
                     /locus_tag="PKX09_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU17136.1"
                     /translation="MKKFLIMITLPTLLSAQAFDSSRAVGTPNIMYDLGIFIEPARES
                     APLPKGVANPNLVPIQNQIDSLLSAFSIMNNQIRSLEQANDSLQKKVNELSKRIFLSP
                     FASLTVPVQIYSRHEGERFLQRGRAAYQANNYPVAIDYFLKVLASNMPDATIGDTYFW
                     IGYCYVQMQDEYLAIEYLKKVAEYPLSEKLDDALFLTGITYQKLGNPQMAEIFFSRLI
                     KRYPEGNLARLAALELKRLKGPD"
     gene            29587..30930
                     /locus_tag="PKX09_04610"
     CDS             29587..30930
                     /locus_tag="PKX09_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU17137.1"
                     /translation="MTRPHLKISLLLLLTITFLQGESVTRLSFSHPGYMMRIPVSSVY
                     RTPYVFRTGFASDTYGFSDTLIAKGIFFEADLTNTFKVGLTTIQGISGTRPVEFGLHF
                     QKRVFTYGDISFTAGVHDMVLRQGEKNLNIDTETLSIFGLLSNEKQFTNNILHTYMGF
                     GTGGLASTFGTDSTANMGVFAGILVHTNIRKDQGGVSFMVEFDGGGVNLGTRIPLTQD
                     YNLSIGINDVTHLYNFGSKNYSTTPQNEHPSLSISLDFTIPRIKPENIKRRALEGMVD
                     PHTKAILDVEEQFAAKLDSTLKAADFEIAKLRDSLRVYETQIKYLTSQVAQLKQKSAV
                     LEDSVRSIKLAKYAMERNINLALKHLSNSLRYFYEGDYQQALKEVDAAIELNPNLALA
                     YARRGSIYYKMGDIERASINWNLALKIDPEYDDVRNILRAMSENRLRSVGEEQKE"
     gene            30939..31733
                     /locus_tag="PKX09_04615"
     CDS             30939..31733
                     /locus_tag="PKX09_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOU17138.1"
                     /translation="MDIATIFGIIAGTVLIIMGISGLTSDFKIFWDLSSVFIVVGGSF
                     ASTMISFSVKEVFSLIGSIIAVFKKEKIKMTDEVETIVELATVARKSVQDLEKALVDV
                     QNPFLRDGLQMVVDGYNPQEIRDILETRIEYRTLRERGEANVLRTMGKYSPAFGMIGT
                     LIGLVVMLYGMAQISSAGLDPMQVLGEGMGAAIITTFYGVILANLIFNPMAVKFETRI
                     QKQNILQMMLIEGILLLQARKHPLIIREKLNSYLRPRDWKNPEEIK"
     gene            31736..32425
                     /locus_tag="PKX09_04620"
     CDS             31736..32425
                     /locus_tag="PKX09_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOU17139.1"
                     /translation="MADEQNPRHKIFSHQDTGDEEPWLITYSDMMSLLLTFFILMFSI
                     STIDPVKLEQIGNSVGQALGGAKPKPTEHFSIKEIYDKVVDVIEQEQLKGVIEVETSS
                     RGVSIKIPSEISFASGKADLDPRIFPILNKLALMIAQSKFPIAIEGHTDNVPIKSDVF
                     PSNWELSSARAAQVVRYFVGLGLPSDRFRAIGYADTRPRATGRTIEEANQTEEQRAQN
                     RRVEIFFLTIG"
     gene            32441..33844
                     /locus_tag="PKX09_04625"
     CDS             32441..33844
                     /locus_tag="PKX09_04625"
                     /inference="COORDINATES: protein motif:HMM:NF019944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HOU17140.1"
                     /translation="MSHFKVKQLILIFSLGLFTLLIAQENLSYLSQKMSLENSIKDRI
                     TEALEKLLDDVKFVVNVKAEIDFAPIEQAEPTETTETPPVGTTPQESRPRATEYVLPL
                     PGFDIPTSVIQSQERIQSQTQLDALPQPATGTAKSTSTTEKKVATSVPVIKRQEINVI
                     LEDGISPEIIENVRQVVAVAAHYDRARGDVISIMTANFKKSPDKDATETIILKNIAEK
                     IDDIERRQTAAEYNARLEQQKQLERQAILRDSLKIDELKKQIAELQAQLQTPQITEDQ
                     RQVTEQQTTTREAELESLREQLRESNRRLQELELSSLETTPPSMLRVRNYGMWYAIIA
                     AIVLLIILLIVLLVNHRKQVKRQELEWGYGSQIPMGPRPTQPILTRQPEETPKPQSPV
                     PPITQPIATPAPPVESAQPAQPSAPPVDFEAQKEEIKSMRQSVISMSVGQPETALRII
                     NAWISQDIQPGKSETES"
     gene            33857..34789
                     /locus_tag="PKX09_04630"
     CDS             33857..34789
                     /locus_tag="PKX09_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HOU17141.1"
                     /translation="MIDAKNLSGLDKATILFKALGPDLSVQLFKGLSRSQMQKIRENM
                     AKIANIPFDVKKAVLEEFYFSFVSEKFIPAEEETKKPFEFMLKLTDEQIAYLIASEPP
                     IIMALAIAQLPVERQVNVLQKLDPTVQPKVLTEMGHLTEIPLEGVVSIANNLKDKSAF
                     IPRASEFKRGGGQDVAGILSKIPPRDEKRFLEFLSKEAPELVDQIKRYYFSFEDLPKL
                     PPNILAEVLKSVEISEIAYALKGQPDEIRDLFKSLLPQRSQIILEDEMKLLEGPQPRR
                     KVEAAQKKIVEKLRELEKEGRFNLEDFMDTDFIE"
     gene            34881..36014
                     /locus_tag="PKX09_04635"
     CDS             34881..36014
                     /locus_tag="PKX09_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17142.1"
                     /translation="MKIIKTLLITLLLGGFANLKSDTFEQQEERVQIALKAMYNREYK
                     RAASLFHEVLNSTPFHPMGPLGALAVEYYQNEGLIGYHQRNQQFLVDIEQALQIYADQ
                     IKQNPSHSEYFFFYGTVMGLRARIQLAEKNYFGVLTSGYNAIRFIKKTEKLYPDQPDF
                     QLPVGLFNYYVSISAPYMKIASRLMRESGSRSEGLQAIKADADYGNYGRYEARSLLAY
                     LYLYFESNYSGAYEYARKMAEDFPKNPYYAALTADAAIQIGKLDQANYYLKRIEALLP
                     TLPLKTQKDYRGILHLIYGSLALAQGRLGEAESNLIEFIKSYDYELDYDLGNALVRLG
                     NVYDLQKRRSEAIACYRKAVQLNNRTAAVSLANQYLKQPYNRN"
     gene            36203..37054
                     /locus_tag="PKX09_04640"
     CDS             36203..37054
                     /locus_tag="PKX09_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOU17143.1"
                     /translation="MNGQIPVLMVNGESLAAAWEKSLVELYQKGCDIKTEYDKPSDPL
                     SKDCTMMIVVQNPLAEPMIHCDFPGGLTDLQEYVLEVLEGIKDHCIRSAENPADARWE
                     YTYHQRLFNYQVPGSAKVYDQIAIMVEKLTKVPHTRRAQAITWKVWEDNDCYDPPCLQ
                     SIWCRLLKDDNSIWTLNTNVRFRSNDAYKAAFMNMFALTQLQVEIANRIEQAIGEKIN
                     IGRYVHQADSYHLYGSYFDEFQNRFLKALETRSFEERTFRYADVRSIMTEAIPQIRAK
                     AARMGRE"
     gene            complement(37262..>37516)
                     /locus_tag="PKX09_04645"
     CDS             complement(37262..>37516)
                     /locus_tag="PKX09_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013456682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOU17144.1"
                     /translation="IGKSFFYGQLSGFVEVISGIIGAYLVLTIQALLPYALSFAAGAM
                     IYVVVEELIPESQKDVSTDVATLGAMLGFTIMMILDVALG"
BASE COUNT        10922 a         8245 c         7713 g        10236 t
ORIGIN      
        1 gtcggaaaac cactcctaaa agtattcaat atcatcaatg aatccaccgg gaaaacacca
       61 cccaatccgg ttaaaagaat cttaaaagaa ggtctagtta tagagttagc caatcataca
      121 gttttaatct cgcgcaatgg acagaaaata ccgattgatg acagcggcgc accaatcaaa
      181 gacgaatatg gcaatatctt aggcgcagtt ctggtttttc gcgatataac cgaacagcgc
      241 aaagctaaaa aagaactcga gtttgaaaaa actttaatag acgcaatgct gaacaccttc
      301 cctgattata tctatttcaa ggatttaaat agccgttttt atcgggtcag caaatcttta
      361 gctaccgagg tttttggatt gccagatcct aaggatgcga ttggcaaaag cgatttcgat
      421 ttctttcctc ctgagttcgc tcaaaaagat tataacggtg aacaagagat catcaaaact
      481 ggcaagccaa tcattgaccg agcagaaaga aaaaattggg cgaatggaac cgtgtcttgg
      541 tcctccatta caaaaattcc actcaccgac aaagatggtc aaataatcgg gactttcgga
      601 gtttctaaaa acatcaccga aagtaaaaaa ctggaagagg ctctccgaca atccgaagaa
      661 aaataccgca aacaattcga agaaagcatt gatgctattt tcttagccga tgccgaaacc
      721 ggtatcttga tagattgcaa caaagccgcc gccaaattag tcggtcgaga aaagtcggaa
      781 ataatcggac aacaccagcg gattctgcat cctccgtcag aaatcatcaa tgggcaaagt
      841 cgaacatttc ttgagcataa aaaagccaaa gagggacaaa ctttagaaag ccagatcatc
      901 actaagagcg gcgaaatccg agatgtcgaa atcaaagcca gtgtttatga aatcgaaaat
      961 caaaaaatag tttacgccct atttcacgac gttaccgaac agaaacgaat cgaggcggaa
     1021 ctcgctaaag agcgtaacta cctgcgcgtt ttattgaaca ccactcccga ccatatttat
     1081 tacaaggaca gagacagccg attcttattg atgagcaaat cccaggctga ccgatttgga
     1141 ctctccgatc ccaaggaagc catcggcaag accgactttg atttcttcac tgaagaacat
     1201 gcccgacctg catttgagga cgaacagcgt atcattcgta ctggtcaacc gatagtcgga
     1261 atcgaagaaa aagaaacctg gccagatgga ggaatatcat gggtttcgac aactaaaatg
     1321 ccgttgctcg atgaaaaagg cgagattatc ggcactttcg gaatttcaag agacatcact
     1381 gagcgtaaaa tagccgaaga acaattatta aaggcgatgg aagaagtaaa aaacagtgag
     1441 gataagctca aaaatatcat taatggttct gcaatccccc aattttttat cgatgaagat
     1501 cacaaggtcg tctattggaa taccgctttg accgaactaa ccggcataaa agcagaggaa
     1561 atcgtcggca catccaatca ctggaaagct ttttatcaag agcaaaggcc ttgtctcgcc
     1621 gacttactcg tagagggaaa tatcaaaaac ctacccgagt ggtattctga taaataccag
     1681 aaatctcatt tagtcgagaa tggtttcgaa gctaccgaat ttttcccaaa tctgggcaaa
     1741 gatgggaaat ggttattttt tacggcggta gcgatcaaga attcaaaagg tgaaattatc
     1801 ggatctctgg aaactctgga agatattacc gaaagtaaac gcgccgaaga agctctcgcc
     1861 caggaaagga ctctacttaa aaccgtcatt gaaaacattc cggataaaat ctacgccaaa
     1921 gataccgaag gacgctttat tctctgtaat aaagccgtcg tggaacggat gggcaagtcc
     1981 gatgagaaag aaatcatcgg caaaactgat ttcgacttta tagatcataa attagcaaat
     2041 caatttcggg ctaatgaact ggaaataatc aaaaccggta tcccgctact taatcacgag
     2101 gagcctctag aacaagcaga aggcagaatg cgctggaatt ctaccactaa agtaccactc
     2161 agggatttac agggtaacat aatcggtatt gtaggcgttg gtcgagatat taccgaacgc
     2221 aaacaagccg aagaacaacg cgaaaaattt atcgccgagt tgcaggaagc actcgacaaa
     2281 atcaaaaccc tgaaaggctt aatcccgatc tgcgcctgct gtaagaaaat cagagatgac
     2341 cagggctatt ggaatagtgt tgagtcctac attaaagacc atgccgaagt ggaatttacc
     2401 cacggcattt gcccggattg tatgaaaaaa ttatatccaa attactgtaa agacgataac
     2461 gataaaaaga agcctgagac aaaatagtct attccagacg ttttatcaga atctgatagc
     2521 catcggcttt gacaacttta ttataaaatt ctttgaatgc gggataaatt tccggcgtaa
     2581 cctcggattc cttatttatc attagccgag tgccttttaa aattctatca ttataatcca
     2641 gcgtaagccg ataatcggca atcggacagg aacattcgta atacggagta aattccaacg
     2701 gctgatagcc agccggaaag gtgattgtga tttcttcgct aatcgtatcg gcatttggcc
     2761 aataagtaaa cggataattt cttttatcat acgatagcgc cctaattggc gaaagccgat
     2821 cagcccaggg gatgcgtaag atgagaaatt ttccggcttc agttaaataa tctggaacct
     2881 taaaagtgta ttcataatcc agaatcggtt taattgagtc gaggtcaaat aaattaaagc
     2941 taatcagttc cacattggga aaatcttgag ccaacgattc gattaattcc ttatcgcgat
     3001 cggcttggct cttgaatcgg taaatcgcac gaaattcagc cgtctgagca cctttacgag
     3061 tagtgtgaag ctcaacctga atactattat cggtttgaac ctcgactttg gtgcgtcgtt
     3121 gccgaacact ccccataaaa aagtggcgcg ttagataaaa cggttggcaa cccttcttga
     3181 tttcgagtgc aaaagcccct tgatccgcca agggcaccga accgaccgga aaattctgcg
     3241 ctgtcaaatc gagatagagc gttccctctt gagtggcaac gctgacgatg gcatgattaa
     3301 aggctatcga aggcaacatg tgtttattca agccttcata gcgagcgttt accaacacga
     3361 aattggctgt gaatcccgct gagcgcaaca tagcgattcc gagggcggct ttatccttgc
     3421 aatcaccgat gcgggttacg aatacatcgc gggctttttg aggaatcaag ccggattggc
     3481 ggaaggatac actgctatag cgaatatttt cggtgatata attataaata cgggtaattt
     3541 tctcctgttc ggtcagcggt tcattttccg gtaataaagt cttgagaaat tcgtcaatct
     3601 cataacatga acgagttctg gtttctgcca gatcaatata ccagttcacg atgtaatccc
     3661 agtccgggat gctggaaatg tgcagtattt ttccgatatc tgctagggga ggcatattgt
     3721 attcctcttt tatggcggga acgtcgttca ggcgccattc atagagaatt ccatccggcg
     3781 tttccttgat agccgaaggt tcatccggca taaattgacc tttatactga aatttcacat
     3841 tcggcggaat caataaggca tagcaaactt cccgcacagg aaaaaatgta ttgaaactga
     3901 aatcgtccca gaaatgctgg gatagtttgc cggagtaata attgcgaatt ttccatttaa
     3961 ggtaaacggc gtcaccgggt tcgagtgatt taaatacaat atagccccag ctaacgtcag
     4021 catcaatttt agagccatcc ttttttaaag tttgcgcctt ttcgacaatc agttcttcgt
     4081 tataagcgtt ataaggaatc cggtattctt tgaaagcatc gattccttcc agattaaaaa
     4141 ccgtcgccag aatttcctgg ctcatttcag ttgcgccacc ataaacgacg cgccgacagg
     4201 cattgtatag aataaccgcc ttttcaccgg gatactgctc cgacgatggg caattgcgaa
     4261 aaagactatc aatatccacc gtatcgaaat tgttaaaaat tgatgcgcgt ccttgcaatt
     4321 gccttaattt atcgcgcgca tcataatcag tcggactcaa ttccagcgct cgctggtaag
     4381 acttaatagc cgcagcggat tgactggacg aacgttggat ttcggcgcga gtgccccagt
     4441 agccggcaca ttgcggacag atttggagag ccttctcgat ataatccaaa gcggtggaat
     4501 agttatgcct attatagaaa ttttgcgcca tttgatagaa aaggcttgga gaagccggat
     4561 aatattcaat cgcctgtcga taggtttttt cccagttctt aaggtcagcg gcatttttat
     4621 aataatcggc taatttgaga aaagccgtct gctcggtgtg gtttttcacg tatttacgta
     4681 aaatgggaat agcggctttg ttattttgtt taacgtaagc ttcgaggttg gctttggttt
     4741 cgacaatgtt ccagttatcc ggaaattgat ggtaagcggt attggcaatc tcgattacct
     4801 tctcggtcag accgcgtttg gaataataag cgataagttg caattgtaga ttgggcgaat
     4861 ccggtatcaa ttgcgccagt cggtcgagta aattcttggc ttcatcataa tttttctttg
     4921 cgaggagctc agagattcgg tattctaaaa cgcctggaat atccggatca agctggtaaa
     4981 ttttttcata agcggacttg gcttcgtcgt atttttcgcc gcgcaagtaa gcctcgatca
     5041 aatgtaaata aatcatagca ttagtcggag cgcgtcgcag agcctcgcgc aaaatcaatt
     5101 cggactcgac cgctttatcg ttcaaagcat aacattccgc cagcagaaga tagttttcga
     5161 gatagtcggg atgttctgca attttcgtca gaaaaaattc ctcagcgaag ttcgggatgg
     5221 gctctttcac ggcaccaggg tgtgatttat aggaatgcgt caacgcagaa atttttaggt
     5281 cggtcactga attgccagcc gggtcagtaa atcgtgccgc gaagttgcac tgcgtaatct
     5341 ctgaaaatcc gcacttgatc aatagacgat tccaacccgc ttgcagttca gttttgacac
     5401 tataagtatc gaatccattg ttattctcat cgaagcattc gatcattagt tcgtcattca
     5461 gaaaaacctt cagcgaaccg gatgttccga ctcgcaaatg aacagtttgt ttctgcggtg
     5521 aaaaaataaa agtattgccg taaaaaactg cgtcgttatc ggcaaagtac cgacgtaaat
     5581 caatccattt gtcataacgc acttgcggaa tccggaacca cttaatcgca atatcatttt
     5641 gtccgcgata aactgccgcc gtgtcgaatt cggtctccgg cggatagact ttatcataac
     5701 cggaagccga aatgttatcg aatgggccaa tcaacatcca atcggtgatt gcgtcaattt
     5761 tattgaagta gaaaagcgcc tttttcagat tggcatgttc gttatagtag tgccctaact
     5821 tttcattggc attgattcgc atcgtagaat ggcgggcagt gcgcgctaaa ttatcgaaga
     5881 gcatctggat accagctgat ttatattcct cggcgttggt taatcggggc gccatcatag
     5941 cggcaaaaat ataaggttca aaatcggctt cggtttcaat gactttggcg taggtttgcc
     6001 aggcttccag atatttatat tgcatttcat aaaggtaagc taacccgatg taagcacgcg
     6061 tgtcgttagg attggcttgg atggcttcct gaaatttctg ttcgacgagt ttctggtcgt
     6121 ttcggttcca ggcttgccag gcttcctcca cagattgagc actggcggaa atattgggga
     6181 aaagagactc gccgaatagc aaaataatta atatggtatt tctaaaaaat cgggtggctt
     6241 tcatttttat tttcctgttt ttaggcaatt tcgataatac aagtaattat caaattaata
     6301 attcccatac taccgaataa taaatctttt tgccgagaaa tgaaacaatt ttatgatcta
     6361 tctaaatcgg gaaaaattgt gctgtttttt aaaacaaacc ctctatggaa tttccacctg
     6421 ccagagtaaa gaatcctgcg cttggcgcgg actatgcaac cagaccgcca gatgatagcg
     6481 gtcaccggaa taaccagtaa aaattgtcag aaaggtataa tgacaaatac atctgcaata
     6541 gcagtgttcg ctggaaatac cacagacatc tctgatcata atcttgagac tgtcaccgga
     6601 ctgctcactt tcgacatcga acggcgcaca gcaaatgtag gtaatgccgg taaaaatctt
     6661 tagcgtatcg tcgctgattt cccagcttag cgaatcattt tgaatcgccg cttttggtaa
     6721 aatgtcaccc cccttgtatt cattacatcc gccgtattcg gttcgtaata acgaaagaat
     6781 agcggcagtt ggtggttcac tgccattatc attacaagcc ataaatccga caattaacat
     6841 tccccagatt agtaggactt tgaattgttt taacatagtc acctcctaat tgcgactttg
     6901 ctcaaagata aacaaaaacc tgaaatattt aaacaattat ttattcaaaa ccgagtagtg
     6961 ggcgattaaa ttttttccgg atattattga tgccctgcaa aacgcccggt ctttggacta
     7021 actttctttc gagcctgtat ttaccgggaa aattcattaa aattataccg accagaatcg
     7081 ttaagatacc ctgccctggg ataaacagta gtaaaaatcc gactatcagg aaaataagtc
     7141 cgattaggtt tttgactatc agtaatatta cacccaagag cggtcgcttg gtaatcaaga
     7201 agcttttacg acgtttggga ttggaaaaat aatccgtcgg aatcatcaga atcagtaccc
     7261 ccattaaaag aattgtacca aaaaatgtga tcaaagaaat caaaccggcg tatttcaaca
     7321 aagtcgtatg cgctttaagc caatcataaa gtagcggaaa acgttccaga aaattcagag
     7381 ccataggaca atcggggatt accggaaaac aaaagatgtt atagatttca tcacgttaaa
     7441 aataatttaa aatgaaattt tattgaacag tttgccctat tagatatacg cattaaccga
     7501 gttgttttat taatgattat atctctaagg ttcaatgccg agaataagaa gcggtacagg
     7561 agggctgttc cgtccttgtc tcataacatt atagacttct ccctcgtaat atgcaattcc
     7621 gcttgacatt tgggactgca tattcttagt aggaagaatt tctatgccta agtttatcat
     7681 gccgcctgaa taaaatagca tgtgctttac aaacaaatca tatgcaacaa aagcatattt
     7741 accaaattgg acttcaacag caactttatt tttgacgaaa tctgtctgat tatagctaaa
     7801 aattgggttt ttctctccgt gttctatgag aaatttcttt tgttcacctg caggcattaa
     7861 taaacttttt tccatcagtt cccgatttag agtgatatag taattataac gactctcgtt
     7921 ccaatttctt tttgaaaatt ccctgtcaaa tgctttgttt agttctattg gactatataa
     7981 ctgtgtcccc gcttttctat tttcattact gacttttgtt tttaatccct cggcatttat
     8041 gttcttgata acacttttga tttcattata aagatcttta tgatgcacaa tcaaatattc
     8101 ttcgccgttc aaatgcgaat attttttaat tattttcatt tgctatatcc gttaaatttt
     8161 aattttagcc attcattcgg cacattcgca actttatcat ttttagaagg tttgtgaata
     8221 ggtttattaa tcggtctcac ttttaagtta cctgctttca aagcttcgat tctattttgg
     8281 gcgattttac aataatgggc ttctttttca ataccgatgc aattgcgctc gtttttaatc
     8341 gaagctataa ttgttgaacc aacaccagca aatggatcaa gaacccagct acttttatta
     8401 gttaaggcta aaacacagcg ctcgactagt tcaaccggaa attggcaggg atgttctgtt
     8461 ttctccggat ggttcgattt aacgtttggt acattccata tttcattatc ccaatcttct
     8521 acaacaattt tccagatatc cgaaggattt ttcccaagtg gatttccgga aggctttcct
     8581 ttgttaggtc ctttaaaatg taacttgccc ggatattttg caggaattct aacggcgtca
     8641 agattaaaaa tgtaattatc ggttttggta aaccacagaa tggtttcgta tcttcctgaa
     8701 aatcttttgg aagcatgcaa tccatgctca aaatgccaga taatacgatt gcgaagtttt
     8761 aattgatatt tcttgaatat cggataatag taaatatcaa gcggaaatat ttctccctta
     8821 tctacaaaat ttcctacttg ccagcaaaga ctcccctggt tagaaagaac gcgtattagc
     8881 tgtctaataa tttcttcttg cgtttcaaga tatttctcga tacaggtttt ggtttcatat
     8941 tccttaccaa tattataagg gggagaagtg attattaaat cgaatttccc gtctggcaaa
     9001 ttttggagga tataacaaga atccccattt aaaatttgat attcaatcgc aggatttaca
     9061 taattaaata gagtttcttt tacttccata ttattcgttt tgtttaaaaa ctctgattat
     9121 tttacgtaaa atttacctaa aaggcgaata attaatcttc agaaatttaa aaggtcagag
     9181 gataatgatc aacgctttac aatcccagaa ttttttcgag cggtcggcgt tccggtacat
     9241 accggtcagg aattttaccg gcattccaat cggcggaaac atacagtccg caataacaac
     9301 gtccgtattc cttcacatcc tgctcacggt aaacgcacgg gcaaatgata tcgcgatccg
     9361 attcgcgatc accgaccgcc agacgacatg gacaagccat atagccataa cgttctttgt
     9421 taatcaataa agaattcagc aatcggtcaa cgtgctgacg atctttattg aaaaaatagc
     9481 ctttcggctc ctgaagtttg cgaagcgatt caaataactg ttctgtgttc ataatccgag
     9541 tgcctttttg atttcttccg gacgatagcc gacaataacg ttgtctccga tgacaattac
     9601 tggaaaagca actgtcggtg ttaatttatg aatctcccta atgactgcca gtttttgttc
     9661 cttgtctaac aaatctacgt caatactatc gtactcgatt cccagactct tgaggaaggc
     9721 tttggtattc tggcaataaa tacaggtgct gagagcatac aatttgacct ttttatccat
     9781 aaacgactcc tttcttcggg gttaattctg cggataaatt ttatttcggt gcttttgaga
     9841 taatttcaca atgatattgc aatctgatta ccaaaactaa aacaaatata agcgtttaaa
     9901 attacaaccg gtaaaaatat tcttaaagcg gctttgaaag tttctctcaa tttgatactg
     9961 gctttcggtt gtttgagatg atcgaagtcg ccggtctcag ggttaaggtg gtaatctttt
    10021 ccccactcaa cataattttt cataatttcc cgtaccgcag atgcgatata acgcacttcg
    10081 acttcagtca tagtcggatg caacgaaatg cgcacccagc cgggcttcaa actcaagttg
    10141 ccctgatcaa tctgactcat aattgcttta gatcgttggt cgtcaatatg tagtaaaaca
    10201 tgaccgtaag ttccagcgca gagacagccg ccacgtgttt ggataccgaa gcgatcatta
    10261 agtaggcgta cgatcagatt atgatgaact ttcagactat aaaacgaaac aaaaccgatg
    10321 cggttatggt tttgagattc aagaatgttt atatcaggga ttccagccag ttcatttagc
    10381 aggatttcgg tcaagtaatg ttcctgtgcc agaatatttt caacgcccac ttcttctttt
    10441 agtaaaattg ccaacgcggc gcgaattgcc tgtaaaaatc cgggtgttcc accatcttcg
    10501 cgcacttcaa tgtcctggaa aaaacgatgc tcgccccagg gattagtcca aagtacggtg
    10561 ccgccgcccg gttgatctgg aacgcgatta ttataaagct ttttatcgaa aacaatcact
    10621 cctgaggaac ccggcccgcc cagaaattta tgcggtgaaa agaaaatcgc atctaatttc
    10681 tgttccggtt tgggcggatg catattgatt tcgacatacg gcgctgaagc ggaataatca
    10741 atgaaacaca ggcgtccgaa ttggtgcata atctctgcta attcgttaac cggtgtgatt
    10801 aaaccggtaa cattcgagca ggcggtgaat gagccgatta ttaatttccg gtcacggttt
    10861 agttccaaaa tattacgcaa atgctcaaga ttcggtaacc cgtccgattt tttacaaatg
    10921 agtgaggtgt cacagcaaca ttcctcccag gtggtttggt tggagtgatg ctccatatgc
    10981 gttagaagta tcagcgctct ctcttgaggc ggtattgata ttttttcctt gaatttatca
    11041 ggtacacgta accctaaaag acgttggaat ttattgataa ccgccgtcat cccgaaccca
    11101 gccagtaata ggacgtcgtc cggaccggcg ttcacatgct gtttgataat cttatgcgct
    11161 tgatggtagg catcggttat gattgtccca gtcaaggtcg catcactatg agtattggca
    11221 acgtaagggc ccaaaacatt agttatatat tcctcgatcg gtcggtagag tcttccactg
    11281 gccgcccaat cggcatacag aatcggtcgg caaccgcccg ggaaattata ctggtaatca
    11341 atgccgataa tattacggcg aaaacagcta aattgttctt caagaccagt tttatttgac
    11401 actatcttca cccaaaaatc aatgtgtaaa tttatgaact tagaatttga taaaaataaa
    11461 aaaattcatc agaagaaagc cctcatcatt ttattaaggc taatagcaaa tatttcttta
    11521 caaaatgctt gaatttggtt aatctaaggg tagtcaaaat gaataagcac tttctgatat
    11581 gggcgatcgg tctctggtta agcgcctgtg ctactcatcc cacgattagt ccggtgccac
    11641 tcaagccggg cgaaacttat accggggtga ctttctctgt tgaaaatgtc gcacctattt
    11701 tcgtttatcg gcgcggcgtc tcagagcgga gtgatattgg tttccgagtc gggttgccgg
    11761 tgtacggtag tggcattgac ttctcatatt tactcttcca ccacgataac attgccgatg
    11821 tacttaattt gagtttctcc gtaaatccta attccaacct cgatatgacg tattattcgg
    11881 tacggacatt tcccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngagtac
    12001 ggatttccga cagaatattc gcgcttgact ggaatctcct gccaattatc attaagtatg
    12061 caaattttcg gaaagaaatc cgcaaaataa accgaataat taaattttaa cttcgattgt
    12121 tagagaaatc agtattcacg tttctaatgt atctatgaat taaatttatg atatattccg
    12181 ctgaaaatta attgactctg gttcttcttt attctaaatt gatatgctat gaataagttt
    12241 aaactcacca ccaatttcac accgaccggc gatcaaccgc aggcgatacg ggaactaacc
    12301 gaagggatat tacgaggcga caagttccag actttgctcg gggtaaccgg aagtggtaag
    12361 acttttacca tggcacaggt cattcagaac gtgcaaaagc caactttggt catttcccac
    12421 aataaaacgc ttgccgcaca attatatgga gagtttaagg cgtttttccc cgaaaatgcg
    12481 gtcgagtatt tcatttcgtt ctatgattat tatcaaccgg aggcttacct gccggttacg
    12541 gatacttaca tcgaaaaaga ctcctcgatc aacgatgaaa ttgataaact gcgcctcaaa
    12601 gctaccagct cgttaattag tcgccgcgac gttattgtgg tcgccagcgt ttcctgcatt
    12661 tacggtatcg gttcgccgga agaatacact cgctttattg ttactcttaa aaaaggtgac
    12721 cggattggca tccgtcagct tttcggcagg ctggtagata tttattatgc tcgtaacgat
    12781 ttagtgttgg agcgcggcaa atttcgcgct cgcggcgaca tcgtcgagat tcgtccggct
    12841 tatgaggatt acgcctaccg catcgaacta tttggcgacg agatcgagca tatttatatc
    12901 tgcgatccgc tgaccggcga agtcaccaac gagcaagaaa tggtcgtaat ctttccggct
    12961 aagcatttta tttcttccga agaaacggtt cagcgtgcca ttgtttctat cgaggaagag
    13021 ctagaggcgc gccttgaaga attacgttct gccggtaaat tattagaagc ccagcgcttg
    13081 ggacagcgta ctcgcttcga cctcgaaatg attcaggaag tcggttactg cagtggtatc
    13141 gaaaattatt cgcggcattt ctccggtcgt aagccaggtc agcaacctta tactctgctg
    13201 gactttttcc cgagggattt tttactaata attgacgaat cgcacgtcac tctacctcaa
    13261 ttgcgcgcta tgtataacgg cgaccgatcc cggaaggaag tgctggtcga gtatggcttt
    13321 cgcctcccgt cggctctgga caatcgtccc ctcaaattcc acgaatttga aaagatggtc
    13381 aaccaggcag tttttgtctc cgccactccc tccgattacg aattggaaaa atgcatcggc
    13441 gtagtcgtgg agcagattat tcgtccaaca ggtttgctcg atccggagat tatcgtaaaa
    13501 cctaccaaac atcagattga cgatctaatt gacgaaattg cggctgtcac cgctcgcaaa
    13561 gaacgcgtgc tggtcactac gttgaccaag agaatggcag aggatttatc tgagtatctg
    13621 cgcggtatgg gacttagagt ccgctatttg cattccgaga ttaaagccct cgaccgtgtc
    13681 agcattttgc gcgacttgcg gctgggaaat ttcgatgtgc tggtcggcat taatctcctg
    13741 cgggaaggtc tcgacttgcc ggaagtatca ttagtcgcga ttctggacgc cgacaaagaa
    13801 ggatttctgc gttccgagaa atctctgatg caagtttcgg gtcgggcggc gcgcaatatc
    13861 aacggtcggg ttatctttta cgccgatgaa atcaccgaat ctatgcgcaa agtcatcgaa
    13921 gaagttagcc gtcgccgcaa ggttcaacag gattataaca tcgcccacaa tattcagcct
    13981 cgcaccattt ataaatccgt cgccgaagta atgcagacca ccgccgtggc tgacgttaaa
    14041 ccgactccaa tccagaccga ccagccggtt ggcgaccgct accgcgctaa aatggaaacg
    14101 tctgagatca tcgaactact tcgtcgcgag atgctcgccg ccgctgaaaa cctggaattc
    14161 gaaaaagccg ccgaactgcg ggacgaaatt gcccgcctca ccggccagaa gaaaagccta
    14221 tctcgcgaaa atctgcttta gatttttccg actaaaccga tttgcccaag tataaaattt
    14281 ttaaatgaat tgcttatgta atccaacctt ttccttgttt caataatcaa aactcggtag
    14341 attactacga cattttgact tactctaaaa tgattaacat ttttatttta ataaacttta
    14401 agatgaatta aatgaaagtt ctaatcgtcg gcagtggtgg tagagaacat gccttggttt
    14461 ggaagctgtc acagtcacca aatatagcta aaatctactg tgcacccggt aacgccggga
    14521 ctgccctttt ggcagaaaat attccgctca aagacaacaa tctagacggg ctactaaaat
    14581 tcgcactcga gaactctata gatttaacgg tagttggtcc agaaatccca ctggtaaatg
    14641 gcatagtaga tctatttcgt gctcaaggtt taaagatttt cggcccaaca tctgccgccg
    14701 cccgactgga gggcagtaaa gtatttgcca agcaatttat gagtcgccat caaattccga
    14761 ctgccgctta ccaagtctgc gattcacgcg ccgcaatgga atctgcaact catttcaaga
    14821 cttttcccta tgttatcaaa gttgacggat tagcggctgg taaaggcgct ctaataattc
    14881 acaacgacga tgacttaaga gaagcttttg aggcaatctg ggatgagaag cgttttggta
    14941 ccgctggaaa taaagtcgtt gtagaagatt tcctgctcgg cgaagagctc tcggttttcg
    15001 ttatcactga cggtgaacac taccttatcc taccaccggc tcaggatcat aaacgcatcg
    15061 gcgaaaatga taccggacct aataccggcg gtatgggtgc ttacgctcct gcgccgcttg
    15121 gcactccgca agtaatgcac aatgtcgaaa aacaaattat cattccaact ttgaatggtt
    15181 taaaagctga gggaatacct tataccggtg tactttattg cggcttgatg attaacggaa
    15241 ctcagcctta cgtggtcgaa ttcaacgctc gctttggcga tcctgagacn nnnnnnnnnn
    15301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt aatgcccact ttttaaccaa cggcggtcgg
    15421 gtattagcag tgactgctct ggcagataat ctcgaaaaag ccattgccag tgcctatgag
    15481 cgaatcgctg aaattacctt tgagggcgct tactaccgcc gtgacatcgg ccagaaagga
    15541 ttactccgcc aaaaagattt gttggaacaa atttaagaaa agaagcataa aatggacaac
    15601 gaacgcatta ataaactaca gcagagtttc ggcattctgg gcgaatccga ttacataaag
    15661 gaaatcgttg aaactattga tcaagtagcg cgcaccgata ttaccgtcct gatttgcggc
    15721 gaaagcggca ccggcaagga ggtcgtcgct aatgctattt atcgccacag cttgcgcaag
    15781 catcagccct tcgtcaaagt caattgcggg gcaattccag ccgggattct ggaaagcgaa
    15841 ctctttggtc acgtcaaggg ttctttcacc ggcgcaatcg aagaccgcaa aggctatttt
    15901 gaaactgcca atcacggcac aattttcctt gatgaaattg gcgaaatgcc gccggaaact
    15961 caggtcaaac ttctgcgagt gttggaactc ggcgaattcc taccggtggg cggctccgaa
    16021 gtccgccgcg tggacattag ggtcattgcc gcgaccaata aagaactcgc cgctgagatc
    16081 gagcgtggaa attttcgcaa agacctttat taccgcataa agactatcac catttatatg
    16141 ccgcccttac gtaaacatcc ggaagacatc ccgcttttag ttgaaaaatt tgccctggac
    16201 ttcgctaatc gcaattcgat cgcttttaag ggtttttcgc ccaccgctct gaatctattg
    16261 cgcgaatatt cctggccggg caatattcgg gaattgaaaa attttgtcga gagtatgatc
    16321 gttctggaaa aaggcgaggt aatcactagt agtatggtcg agaagcattt gctgggcgag
    16381 ccacgccaca tcccttcgcc tcatctgccg atgaaaatta gtaaaagtgt cgatcaagcc
    16441 gaacgtgagt taattctcca acaaattatg gtactccggc aggaactgca tgaaatctat
    16501 gaagctgtga ttaccaaccc tcttccacta aaatatgaag ccttgcgcga attggaaatc
    16561 ccacccagtc tgccccgacc caaagtcgaa gaaaaagaaa tcgggaaact agatagcgcc
    16621 gaaatcatta acgacgagat tatcaatccc gaaatggtgg ggaaagtaac cctcgccgaa
    16681 gtcgaaaagg aattaatcca gcgcactctc aaaaaattca actatcgcaa acgccaagcc
    16741 gccaacgctc tccaaattag cgaacgcact ctctaccgta aaatcaacga atacggactg
    16801 gaagaatcaa ggtaatcgca atgctattca agcgtttggc tcccatcata aaatttactc
    16861 cagcggtttt attatgtttg ataatcaact cctgctggct ttattcattt aaaggcacct
    16921 tgccgcccgg catcaaaagc atcgccatcc cgctttttga ggaccgcacg gctgagttta
    16981 atattcaaca gatagtaacg gataagaccc gacttggttt tatcaaggaa aatttactga
    17041 aagttgtcga agaggataac gctcactcag ttctgtacgg aaccatcttg agcattgatg
    17101 acaaacctct ggtttatgac gaagatgaaa acgtcggcga atccgttacc gaatatcggg
    17161 taacaatcaa gatcgagatc gagtggtacg ataaagtcaa tgaccgcaca gttttcaaaa
    17221 aacagttcat cggctatagt gaatacgacc cgaccggcgc cacgtccaaa acgcgnnnnn
    17281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggctt gattcagggt atcgtatcac
    17401 gcaatgatgt ttccgaaaaa attttcaatt acaaggaaat cctgactcag gaatcgtccg
    17461 tcagcgttag tggactcatc cacgaagaca aacgcgcgcc aggcggtttt gaaatcatcg
    17521 tcaaagatat tcaggtctat caaattgcca aagattaccc gattactctt aaggaacacg
    17581 gcattgaatt tttgatggat aaccgccacc tgtggctccg ttcgcgcaaa cagtgggcga
    17641 ttctgcgcct tcgccacgcg gtttactatg gaattactca atatttgaat gaaaacggat
    17701 ttatccgttt cgactcgccg attctgacac ccaatgcctg tgaaggtact actacgcttt
    17761 tcgaagtgcc ttatttcgat ctcggcaaag cttacctttc gcaatccggc cagctttatc
    17821 tggaaaccgg gataatgagt ctgggtcggg tttatgactt cggaccggtc tttcgtgctg
    17881 aaaaatctaa aacccgcaaa catctcaccg aattctggat gatggacgcc gaagccgcct
    17941 tcgttgaaca cgaagaaaat atgcgcattc aggaagaact cattcgcttc gtaatccgta
    18001 aaattctggc agaatgtcaa cctgaactcg agatattgga aagaaatatc gaacaattgc
    18061 gcaaagccga tgcccccttc aaaaaaatga cccaccgcgc agcagtcgaa tatttgcgta
    18121 gcaaaggctc atcaatgaac gaaacggaag atttgggcgc tcaggatgaa accttactga
    18181 ccgaagggtc agatgtcccg attttcgtcg aaaaatggcc aaaagaaatc aaagccttct
    18241 atatgaaacg cgatccagaa aatcccgacc tcgtactcgg cgacgacctc atcgctccgg
    18301 aagggtatgg cgaaattatc ggcggctcgc aaagggaaga cgactacgat ttactgctcc
    18361 agcgtatgat tgctgagaaa atgccgctcg acgaatatca gtggttcctc gacctgcgta
    18421 aatacggctc ggtaccgcac agcggctttg gcgttggact ggaaagattg atcacttgga
    18481 ttgccggggt gccgcacatc cgggaaacta taccatttcc cagaacgatt tatcggctga
    18541 gaccatgatg accgtttcac caaaaatcat cgccgttctg ggtggccgcg aatgcgacga
    18601 acaaacctat caattggctt ttgatgtcgg tcgcgccatc gccgaacgag gtgcgatttt
    18661 actctgcggt ggacgcactg gcgttatgga agccgcctgc aagggagcag tttcagccgg
    18721 tgggttgacc attggcattt tgcccggcgc agataaaaat gacgccaatc cgtgggtaaa
    18781 aattgcccta ccaaccggaa tcggtgtcgc gcgcaatgct atcatcgcgc gtgcttgtga
    18841 tgccgccatt gctattggtg gtaaatatgg caccttatcg gaaattgcct actgtttgca
    18901 attgaataaa ccgatttgcg gattaaattc ctggccggaa attagcgatc tgcgccagtt
    18961 taatagcgct tccgccgccg tggcatttgc tttcggggaa tgaatatatg gcgatacgcg
    19021 ttgcgattat cgttcaaggc gtagttcagg gagttggttt ccgctggtat gtccgtcgga
    19081 ttgccatgcg ctaccgcttg accggttatg tcaaaaattt acccgacgga gacgtttatt
    19141 gcgaagtcga aggcgaggaa ggaatgatca atgactttat cagcgaactc aaagccggac
    19201 caccattaag tcacgttact aatgtaattg tcaataaatt acctgacttg gtcggctata
    19261 aaagtttcga gattagattt tgaaacaaat aataaaaatc ggcctggcgc agattaatcc
    19321 cgttctcggt gatttgtcag gtaatgctgc aaaaattatc aactttattc aagaggctga
    19381 tcaacaggga gttaacttac tgattttccc ggaattggca atcaccggct atttgccgca
    19441 agatttattg ctgaaccgct cgtttattga ttgcaacttg caatgtttga ataaaataac
    19501 tgccgcttgc cgtagttcga tggtcgtcat tctgggcttc gtcgctcgag aaaacgataa
    19561 actttacaat gccgccgccg tcattaaatc cggtcaattg atcgcaacgc gctacaagac
    19621 acttctgcct aattatgacg ttttcgacga acgccgctat ttcacacccg ctaatgaaaa
    19681 cctgccggta ccaatcgaaa ttggtggtca ttctgtccgg ctcggtgttg aaatctgcga
    19741 ggacttgtgg gatagtaaca gcgatatcaa agtaaccgat caattagttg ttcagggcgc
    19801 tgagttattg gtcaatttat cggcttcgcc atttgaatat gataagcgcg accgccgtcg
    19861 gcaattagtg ctcgacaaag taatcaaact caggcgaccg tttgtactgg tcaatatagt
    19921 tggcggtcgt gacgaactgg ttttcgatgg caattctttc gcactggatg cctccgggac
    19981 gctaatcggt tggggcggag agttcaaaga aaacttagcc gttttcgagc tggatttggt
    20041 tagcggacaa ggccaccgaa tgccctggcc cgaaactcaa cgcgaagcca gcatttatca
    20101 ggctttgact ctcggcgtgc acgattattt ctataaaacc ggtgggcgac aagcgattat
    20161 cggattgagt ggcggaattg attcggcgct tgttgcctgc atcgccgttt ccgctctcgg
    20221 gtcagagaac gttctaggag tatttctgcc gtcgcgatat acttcggccg caagcagaga
    20281 agattctgtt gttttagcta aaaatttgaa tatcagatta atcgaaatcc cgatcgaaaa
    20341 aatatttcag tcttatctcc aggaattgaa accggttttc ggccaaacgt caccagatgt
    20401 cactgaagaa aatatccaat cgcgtattcg gggcaattta ctgatggcgc tggcgaataa
    20461 ataccatgct tacgttttga acactggcaa taaaaccgaa ctggcactcg gttattgtac
    20521 aatgtacggc gatatgtgcg gcgcgctggc agtgatttcc gatttaagca aattagatgt
    20581 ttataaggtc gctggttatt tcaatgaaac ggcggagcgg ctaatcattc cagaacgtat
    20641 tttcaccaaa ataccgacag ctgaattagc ccccggacag gtggacccat tcgattacac
    20701 gatcgtcagc ccgttggtag atttaatcat caatgatcaa aagactccag cggaattaat
    20761 tcagatgggt tacgccactg acctggtaaa cgatattttt gagcgcatta aatttgccga
    20821 gtttaaacgc cgccaagccg cgcccggtct gaaaatcacc ggtaaggctt ttggtttagg
    20881 gcggcgttat ccaattatta atcattttaa agaaaaggga gaaaagcagt gcgagtagtt
    20941 cgctattggc tgacattaat ttgtatagga atagccttgc ataccgcctc accccttctt
    21001 gcccaaaatc caaccgacat tgacggcaca atttggcggc ggatgaaata cgataataaa
    21061 tattactggc tattgggtta tctgcaagga ctggaaaaat cggacgaaat cattcaactt
    21121 tgtctcgctg agcaggaaaa acgcgaattt gccttcaccg ttccttttta tgtagatcag
    21181 atgcgcgccc ggattagtgc ttattttccg cctcataata ttcaagacat cactcaacta
    21241 attgaactgc tgaatacttt ttataaagac aaatacaacc agaagatcaa gatcgaagcc
    21301 gcgctccgca ttgtcctagc tcgccaaaaa ggacagattg aacagtctga tttctggctg
    21361 aatgaagccc gacgcgcgct catccaaaaa taattccaat aatcccggag aaggtacgat
    21421 gaacgaccat accgaatttg aagaaataaa tctggcgcgg catatctatc gttatttgat
    21481 tacaatcggc attgctttat tcggattatt cttaatttct atcgaaccga atgtcctgcg
    21541 ccagtatcgt agttggattc cctcccaaac ttttaatatt tactatctga cctggttcgc
    21601 agtttgtacc atttcggcga tactttgctt tttctgggga gttctctgcc agtttttacg
    21661 aggcaataat tgtctgggaa atgtcctctg gtatgttggc atttttacta taacgctctt
    21721 tttcgccacc gtatttttcc agatcttaaa aaccgatagg gtggtgggat ttgcagtaac
    21781 ttttggaaca ttaataattt ccgcaatggt tacaatggcg atgtcctata ttttctacct
    21841 tttgaatttc aacagccgtt atcatttttg gcttttcaca ttggctatta tttcggctac
    21901 cagcgcctat aattggttat acttgagcgt tattggcaag attccggcgt tttaatttaa
    21961 atcgtcgttt gtcataaaat aggaaggaaa tgatcaagta cattcttgag cgaatgccac
    22021 tcgttggtat ttgtgtagct tatctaagcg gaattgttac cgaccatacc ttgcacctat
    22081 taccagaagt ctggttcatt tttttgctaa ttgacttcct cattggaatt attttctcat
    22141 caaaagctcg atcggttcta ttaattgctt tagcgctctt ttgtctcggc ggaatgaatc
    22201 atgccctgcg tactgtaact ggccctgatc acttaagtca attttatttc tctaatgaac
    22261 cggacagtat aatcgctatg gttgagcagg cagaatttag cggcgacggc aagttaaaaa
    22321 ttcgcttgaa gaatctgcaa ataaaacact ccgactggca gaaatggaac ggcaagttaa
    22381 ttctaaccgt caaagaacct gaaaacaatg tcatttacgg cgattggatg gcattctata
    22441 cccggcttaa tcaaccagct acggcgcgca atccaggcga atttgattac tggcgctatc
    22501 tgactaatca tcatatttac gccacggctt atttagatgc cgggcagact ccgacaatca
    22561 ttggacgaag ccgatccctg cgcagagccg ccaattactc tcgcattaaa gtcgaggaac
    22621 ttattgataa atctatgtca ggcgaacaaa acgccatttt gaaagctttg atcgtcgggg
    22681 tacgcggtga aatcagcgat gaaactaatc aggcattcgt tgatactggc gttgtccatg
    22741 ttctatcggt ttccggaatg cacgtagttt atgtgaccat ggtgattatg ctaattctcg
    22801 gctttctgcg tattcctaaa aaaccgaaaa tggttctggc gattcttggc ttgggattct
    22861 atgccttgat ggttgaccta cgaccgtccg tcaaccgggc agttattatg gcaattttaa
    22921 cattaatcgc ccaaggctgg gaaaagcgag ccaatgtcta taataccatt gccgcggccg
    22981 ccttgattca gtccctgttc gacccacttc agattttcga tatgggtttt cagctctcat
    23041 ttatggcggt aatttcaatc gtctatgttt accggcaatt ggaatattta ttgcctctgc
    23101 ctcttaaaac ttccaacatc aaaaatcgcc gtttgcgtga aatctggcaa ctcttcctcg
    23161 tttctctttc ggctttattg ggcaccatac caattaccgt cttttatttc cagcggattc
    23221 caatcatttc gctggtaacc aatttagcag ttgtaccatt agtcggactc attggcggat
    23281 tgggtttcgg tcaggttatt ctcggatttt tgtggagcgg atttaatttg actttcggcg
    23341 aagttcagac gcttttaatc agcttgttga ctcagattgt caaaatctcc gggcgagtac
    23401 caattgctta tctgacgata ccggcgataa gccttgccgg ggtttatttt ggatatttaa
    23461 ttcttttaat tctgctaaac ctcgataaaa aacgagttca aaaagtagct ttatttggct
    23521 ttcttatcgg cattaatttt ctaatctgga cgcaaaattt atccaaaccg gttcttcagg
    23581 taacgtttct ggacgtcggt cagggcgacg cgattttttt ggaattcccc aatcgcgaaa
    23641 ctatgttaat tgataccggc gatcggactt tccgacgcga ttacggagaa ttaacgattg
    23701 cgccattcct caagcgcaaa ggcttccagc gcattgatta tctggctttg agtcacccac
    23761 ataatgacca tatcggcgga gcgccttatc ttatgcgcaa ttttcaaatc ggcgaaatct
    23821 gggaaccgga catatccgcc agttctcaga tttatcgcga aattcatacc ctcgctgatt
    23881 ccttacaaat tccgatcaga catctctacg ccggggattg ttttccctta gccgagtcac
    23941 tgactgtctt cgttctacac ccgtcaaaag cgttccttga gtcgcatcct cccggattca
    24001 acgattactc aaatgttcta aaaattagct atcgccagat tgacttcctc ttctgtggcg
    24061 atgtcgaaaa gttttccgag agttatctaa atctatggca tacgaccttg caatcagaaa
    24121 tattgaaggt cccacatcac ggcagttcga cttcttcaag tcaaacattt ctcgagcaga
    24181 ttactccgga tttggcagta atatccgtcg gaaaaaataa caaattcagg catccgtcgg
    24241 ctggtaccat tgcccgttat gaagcactcg gcacagaaat tcaccgcact gacctgagcc
    24301 gcgcccttat cattaattca gacggtcata aatactggat caagccatgg tgaatttttt
    24361 tccgtcttag gtttggtttt taatacgcta ttcgataaaa ttgttttagt ttaattatta
    24421 aaatttgatc acggagtaac cgtaaatatg gtgaacaatc aaccggaaaa tccggaaatc
    24481 ctgaaaggct atacggcgca ctggatcgtt cccgtttctc agccgcctat tgaaaaggga
    24541 gtcataatag ttcaaggtca acaaataatc gaggtcgaga gcgcagacaa tctgcaaact
    24601 aatatcgaaa ctcctttagt tgatctcgga gatagcgtca tatttccggg atttatcaac
    24661 gcccatactc atcttgaaca tccaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24781 nnnnnngaga taattcgaac tttccgtgag ctaatccagg atcaaacccc tgagaaaaaa
    24841 attaccaaac atctcacccc atcttacgtt tgggcgactt cgccccaatt attacatgaa
    24901 atcgcattta gtgaacggca tattgctatc cacattgata tgatcccaga agaaaacgag
    24961 tttactctca acggtagcgg tttattaagg cagattttac tcgccaacga ggattttgat
    25021 tatagctggc aggtaccggg tttaacggct attcgctatt ttttcgctaa tcatttctac
    25081 gctcggcaca atatactaac tcatatgaat tgtgttacca gcgatgaaat tgacttcatc
    25141 aaggaatttg gagtaaaagt caatgtctgc ctttgcccac gcagtagcaa aatcttatcg
    25201 caaatcaaga cgccagttga tttactactg gaaaaaggga ttaacctatg cctcggcacc
    25261 gaaagtcggg ttttagtccc cgatctcgat attcgcaaag aaatggtcga atgtgtagat
    25321 acctatggtg tatcccccga gcttgccctg aaatttgcaa cactgaatgg tgcctacgct
    25381 attggatttc acaaagaagt cggtagtttg gaaaaaggta aaattgctcg gtgtttggtc
    25441 gttgattgta gcggtctgac gactactgac ccgtatgaaa tgattctaag tacaccgcat
    25501 tctcctcgct ggttgatctg aaaaaataat tttcttaaga taaaaataaa tacttgttgc
    25561 taatgtcaaa aattttcctt attaattagg gtgagattaa gatttctaag tctcgctgaa
    25621 ttggtcactg aaacctactt aactgaagaa gaaaaaataa gcaccaacga tgacaatcgg
    25681 agtgcaatta attaatggat acggcttttt gtcgcgtaaa atataattct taaccaaagg
    25741 agtcaacatg attagagagc agattctaaa agatttgatt cacctcccgc agaacaaatt
    25801 tcccgtaacc agcgcttact ttacactcga taatattgcc ggcaatcgaa agagtcatat
    25861 cgttgaaata aagaagcaaa ttcgctataa gaaagaaaaa acgtactttc ggcaattaag
    25921 tactgaggag caggaatccg tactatccga ttttgatcgt attttacact ggttcggaaa
    25981 agaattgaat ccagcggaga gtatcagttc aatctgtttc tcctccagtg gcgcaaattt
    26041 atggcgcaca ctcaatttta aaaaaccttt gccccaaaat attctcgtga tccaaccgta
    26101 tccctacatt cgaccctata ctatactttt cagtcgctac cgcaattatg ccgtagtttt
    26161 ggttgatcgt tccaaagcta aaatatttga atcgcatctt ggagagttca gggaacattt
    26221 tcacatcgaa gacaacgccc ccgagtcagt caaagtcggc ggctttaaag gacgtcagga
    26281 acgaaaagtc gaacgcaata ttcatcaagg tgtcattcaa cattacaaag aagtagctca
    26341 agagattttc gaccttcatc aaaagtttga ttttcattgg ataattcttg gcgggagaaa
    26401 agaagtcctg gcagaatttt caaaatattt gcacccatat atcagtgcgc gaatagctgg
    26461 ccagatcgaa gtcgaaccga ccgcaccctt ggcagaagtc ctgaaaaaag tagagaaaac
    26521 cgaagaacaa gcaagaaata attatgaaca agcactccta aaaagcttac tcgatcgaaa
    26581 gtcagccggc cagactgtgg acggtattca ggctgttctc gataaaatcc aggacaattg
    26641 ggttgataaa ctcatcatcc aagaacattt ttttaaaaaa ggattattct gccgcaattg
    26701 tggctttctt agcttaaaac cacttgcctc ttgcccagct ggatgcggaa aattagaaag
    26761 aaccaatgat ctggttgaac atctggttca ccgagctttg aaacagggag tggatctcca
    26821 gttcgttagt agcaatatgg atgattatgc ggggattgcg gctattttac gcttctctat
    26881 cggtggttaa atttcatgat aaatctccat tctcagattg aaaaaataat ttagagagct
    26941 taattattgg ctatttcaat taaaatttta agtaattttg gctaactcgc ttatttacat
    27001 caatttttta cttgacattt taccatttta aaatatatat tctatctgga tgcaacgcta
    27061 tagtgtcaaa ttaaaaaata taggcactgt ttatctgaag gtgtgggatt atgaaagcca
    27121 aaaattttta tgtatctatt attctggcga taagccttag tgcgtgtggt gcttctcgag
    27181 ctaaaccaga aaaaaccggc gaaaaaacaa ctaaagccaa atcggaaaaa gctgaaaatt
    27241 tacccgagtc gcaaagttct aaatcaacgt cttcaggtcc ccagcccaaa atagccacag
    27301 cgccagtcaa agctaccgcc gccgaccaaa gccgcctcat caatgaactt actttcgaag
    27361 taaaaaaact atccgccgag gtaaaacatc tccaatcagc cctgaaagaa ttacaggcta
    27421 aaagccaaat gtgggataat cctctttcca tttacgataa agaaattatc atggcaaatg
    27481 gcacctcgat tttcggcaaa atcatttatc aagacgaaaa aatgattaaa gttgaaactt
    27541 ggatcggtta tctgattatc gataaaaatc aggtggtgag agttgtgact aatatagttg
    27601 agaagccaga cgaacaatat gtgccgcccg aactcgccag cgatattaaa gatgataagc
    27661 caatcaccat ccaagcccct tatatatcaa aaaccggtga accagcgcca atcggtgaac
    27721 cgaccagccg tgttcctaat tgtgtattag tgggtaacat caaagaacgc aaggatctaa
    27781 gcggcaatac tattctctcc ggagaaatta aaaatattgg aggtcgtcgg gctgattttg
    27841 tcaaaatcaa tatcgttttt cgtaagaact ggagcggcga tactgaaacc cgaacagctt
    27901 ttgtggaagg ctcttatgtt acttttgaag gcacgggaat cacgagtaat aattccctgt
    27961 tacccggcgc caccggaact ttcgagatga tcatccctaa ggatttcgga caatttatag
    28021 gttatactta caatattgat tgggaggttt atagataatg ccccgatcat ttaacctttc
    28081 gcttttaacg gttgtgatta cggctgtaat ttgcttcggt cagggtacga gttcttcgtc
    28141 cggtaccccg attcatgatt ccaataaaac gattgatcag aaagatattg aaatttacaa
    28201 tctaaaggaa gagcttgaat atctcaaaaa agaattgaga aaattacagg agatcaatac
    28261 ctctttatgc gattcactgg aatccaaaca tactcttctt gaaaccacat ctgaaaaaaa
    28321 tgttgatgaa aattacaatc tgcgcattta tcaccagact atcgaagatg accgacgtct
    28381 taatcaagag attgttaaag aaattcaatc caccgccccc atcgcagttc catttaaatc
    28441 acctgagccg agtggtgatc cggagttccg agaacgatat aacgaagctt tgaatcaata
    28501 tttcgacggt aatctaagcg acagtcagaa aaattttaaa aaactgctgg aactgcgcca
    28561 ggatcatccc ttatctgata actgccagta ttggctgggc gagtgcctct attcccaaga
    28621 aaaatacgaa gaggcaattg cggcttttca aacggtaaaa gcattgggtg acggtaacaa
    28681 agccgatgcc gctctcttca agattggttt atgctatcta aaattagaca agaaagccgc
    28741 agcttcttcg gcttttaacg atctgatcaa tttttatccc aagagtgact tggtacccaa
    28801 agcttaccaa tatcttctct ctcaagataa attctaacat gaaaaaattt ttaattatga
    28861 taactctacc aactttgctc tctgcccagg cgttcgattc atcccgcgca gttggtactc
    28921 ctaatataat gtatgatctg gggatattta tagagccggc gagggagtct gccccccttc
    28981 caaaaggcgt tgctaatcca aatttggttc ccatccagaa ccaaatagac tccctccttt
    29041 cggctttctc gataatgaat aatcagatca gatcgctcga gcaagctaat gattctcttc
    29101 aaaaaaaagt taatgaactc tcaaagcgca ttttcctttc gccttttgcg agcttgacag
    29161 tccccgttca aatctactca cgtcacgagg gtgaacgttt tttacaaaga gggcgagccg
    29221 cctatcaggc gaataattac ccggtcgcaa ttgattattt tctaaaagta ttagccagca
    29281 acatgcccga tgcaactatt ggcgatacct acttctggat tggctattgt tacgttcaga
    29341 tgcaagatga atatctagct atcgaatatc tgaaaaaagt ggcggaatac ccattatcag
    29401 agaagctaga cgacgctctt ttcttgaccg gaattaccta tcaaaagtta ggcaatcccc
    29461 aaatggctga aatctttttc agccgactca tcaaaagata tcctgaaggc aacctcgcca
    29521 gattagctgc cttagaatta aaacggctaa agggtcctga ttgaacacta aacggagctt
    29581 cgctctatga ccagacctca tctaaaaatt tcactacttt tactgctaac cataacattc
    29641 ttacaaggtg agtcggtaac acgtctttct ttttcccatc ctggatatat gatgagaatt
    29701 ccggttagta gtgtttatcg aacaccgtac gtatttcgta ccggtttcgc cagcgatacc
    29761 tatggattct cggatacatt aattgccaaa ggaatattct ttgaagccga cctcaccaat
    29821 accttcaagg tcggacttac tactatccag ggcatttccg gcacccgtcc ggtggaattt
    29881 ggtcttcatt ttcagaaacg tgtttttacc tatggcgata ttagttttac ggctggtgtg
    29941 catgacatgg ttctccgcca gggtgaaaaa aacctcaata ttgatactga aacactttca
    30001 attttcggcc tgctgagcaa tgaaaaacag tttactaata atattctcca tacctatatg
    30061 ggatttggta caggcggttt ggcgtccacg tttggaactg attcgactgc caatatgggt
    30121 gttttcgccg gaatacttgt tcatacaaat atcagaaaag atcaaggtgg tgtgtctttt
    30181 atggtggaat ttgatggcgg tggagttaat cttggcacac gcattccgct aacccaagat
    30241 tataacctaa gtatcggcat caatgatgtt actcatctct ataatttcgg cagtaaaaat
    30301 tatagcacca cacctcaaaa tgagcatccc agccttagca tcagcctcga ttttaccata
    30361 ccgcgaatca aaccggaaaa tataaagcgg cgtgctctcg aaggaatggt tgaccctcac
    30421 acaaaggcaa ttctcgatgt tgaggaacaa ttcgccgcca agctggattc gacccttaaa
    30481 gccgccgact tcgaaatcgc caaattacga gattcacttc gggtatatga aacccaaatc
    30541 aaatatctca ccagtcaagt tgcccagttg aaacaaaaaa gcgccgttct agaggattcg
    30601 gttcgttcga ttaagctcgc caaatatgct atggaacgga atattaatct agctttaaag
    30661 cacttatcca attctctccg ctatttttac gaaggcgatt atcagcaagc cttaaaggaa
    30721 gttgatgccg ccatcgaatt gaatcccaat ctagctctgg cttatgccag acgtggttcg
    30781 atctactata aaatgggcga tatcgagcgg gcttcaatta actggaatct tgctcttaaa
    30841 attgatccgg aatacgatga cgtccgtaat atattacgcg ctatgagtga aaatagatta
    30901 cgtagtgtcg gtgaagaaca aaaggagtaa aaactagtat ggatattgcc acgatatttg
    30961 gaattattgc tggaacagta ttaatcataa tgggtatttc cggattgacc agtgacttca
    31021 aaatattctg ggatctttcc agtgttttta tcgtggtagg cggctctttc gcctcgacga
    31081 tgatcagctt ttctgtaaag gaggtcttta gtttaatcgg ttcaattatc gccgtattta
    31141 aaaaagaaaa aatcaagatg actgatgaag ttgagaccat tgtggaatta gcaactgtgg
    31201 ctcgcaaaag tgtacaagat ttagaaaaag cactcgttga tgttcaaaat ccgtttttac
    31261 gcgatggtct ccaaatggta gtggacggct ataatcccca ggaaatccgt gatattctgg
    31321 aaaccaggat cgaatatcgg actttacggg agcgaggcga agccaacgtc ttgcgcacga
    31381 tgggaaaata ttcaccggct tttggaatga tcggaacttt gattgggttg gtagttatgc
    31441 tctatggaat ggctcagatt agttccgctg gcttagatcc gatgcaagtg ctcggcgaag
    31501 gcatgggcgc cgctataatt actacttttt atggtgtaat attagccaac ttaattttta
    31561 atcctatggc agtcaaattc gaaactcgta ttcagaaaca aaacatccta caaatgatgc
    31621 taattgaagg aatactatta ctgcaggctc gcaaacaccc gcttatcatt cgtgaaaaac
    31681 tcaattctta cttacgaccg cgtgattgga agaaccctga agagattaaa tagtgatggc
    31741 tgacgagcag aatccaaggc acaaaatatt ttctcaccag gacactggcg atgaagagcc
    31801 gtggctgata acctattcgg atatgatgtc attactactg acttttttca tcttaatgtt
    31861 ttctatttcc actattgacc cggtcaaact ggaacagatt ggcaattcag ttggtcaagc
    31921 cctcggaggc gccaaaccta aacccaccga gcatttttca attaaagaaa tctatgataa
    31981 ggtcgttgat gtcatcgaac aggaacaatt aaaaggagtc attgaggtgg aaaccagttc
    32041 tcgcggcgtt tcaattaaaa tccccagtga aatttccttc gcctccggca aagccgattt
    32101 ggatccacgc attttcccga ttctgaataa gttagctctc atgattgcgc agtctaaatt
    32161 tcccatcgct atcgaaggac atactgacaa tgttcccatt aaatccgacg tttttccatc
    32221 taattgggag ttatcatccg ccagagccgc gcaggtggtt cgctacttcg ttgggttagg
    32281 actgccgtcc gatcgttttc gggcaattgg atatgccgat acccgtccgc gcgcaactgg
    32341 tcggacaatc gaagaggcaa accagaccga ggaacaaaga gctcaaaatc gtcgggtcga
    32401 aatatttttc ttaaccattg gttaaaaaga ggattacatt atgtcacatt ttaaagtaaa
    32461 acagttgatt ttaattttta gcctcggctt atttacttta ttaattgccc aggaaaattt
    32521 atcatatctc tctcagaaaa tgtcactcga aaatagcatc aaagaccgca taaccgaagc
    32581 cttagaaaaa ttactcgatg atgtcaaatt cgtggtcaat gtcaaagccg aaattgattt
    32641 cgcacccatt gaacaggctg agccgaccga aacgactgaa accccacctg taggcactac
    32701 tccccaggaa tcacgcccga gagctactga atacgttcta cctcttccgg gctttgatat
    32761 tccaacttct gtaattcaat cccaggaacg aatacagtct caaacgcagc tcgatgcctt
    32821 accacaacca gctacaggta cggctaaatc gacttctacc accgaaaaaa aagtcgctac
    32881 atccgttccg gttataaaac gtcaggaaat caatgtcatt ctcgaagatg gtatctctcc
    32941 tgaaatcata gaaaacgtca gacaggtcgt ggcggtcgcc gctcattatg atcgggcgcg
    33001 aggcgatgtc atcagtatta tgaccgccaa tttcaaaaaa agccccgata aagatgcgac
    33061 cgaaaccatt atcctcaaaa acatagccga aaaaattgac gacatcgaac gtcgtcaaac
    33121 agccgccgaa tataatgccc gcctcgaaca gcaaaagcaa ctcgaacgcc aagccatttt
    33181 gcgagattca ttaaaaattg atgaactcaa aaagcaaata gctgaactac aggcacaatt
    33241 acaaactccc cagataaccg aagaccaacg tcaggttacc gaacaacaaa ctaccacccg
    33301 cgaagccgaa ctggaaagcc tgcgcgagca attacgagaa agcaatcgcc gcctgcaaga
    33361 actggaatta agttctctcg aaaccacccc acccagtatg ttaagagttc gcaattatgg
    33421 tatgtggtac gcgattatcg cggcgatagt gctactgata attctcctaa tcgtcttatt
    33481 agtcaatcat cgcaagcagg tcaaaagaca ggaacttgag tggggttatg gttcccaaat
    33541 cccaatggga ccgcgtccga ctcagccaat tttaacccgc cagccagaag aaactcccaa
    33601 gccccaatcg ccagtacccc cgattactca acccatcgca acacctgctc caccagtaga
    33661 atcagctcaa cccgcccaac cctctgcacc acctgttgac tttgaagccc aaaaagaaga
    33721 aataaaaagt atgcggcaat cggtaatttc aatgtcagtt ggtcaacctg aaacagcttt
    33781 acgaatcatc aatgcctgga taagtcaaga tatacagcca ggcaaatccg aaactgagag
    33841 ttaaaggagt aagaaaatga tagacgccaa aaacttgagt ggactggaca aagccactat
    33901 tctctttaaa gcccttggtc ccgacttatc agtccagctt ttcaaaggac taagtcgtag
    33961 ccaaatgcaa aaaatccgtg aaaatatggc taaaattgcc aatatcccgt tcgatgtcaa
    34021 aaaagccgtc ctcgaagaat tctatttcag cttcgtttcc gaaaaattta tccccgccga
    34081 ggaggagacc aaaaagccat ttgagtttat gttgaaactg accgacgaac aaattgccta
    34141 tttgattgcc agcgagccac caatcattat ggctctggca atcgcccagc taccggtcga
    34201 acgccaggtc aacgttttac aaaaactcga tcccaccgtc caacctaaag tattaactga
    34261 aatgggacat ctcacggaaa ttccgctgga aggcgtggtc agtatcgcca ataatcttaa
    34321 ggacaaatcg gcttttattc cacgagcttc cgaatttaaa cgcggcggcg gccaagacgt
    34381 agctggcatt ttaagtaaaa taccgccccg tgatgaaaaa cgcttcctcg agtttttatc
    34441 taaagaagca cccgaactgg ttgatcagat aaaacgctat tacttcagtt tcgaagacct
    34501 tcccaaatta ccaccaaaca tcctggctga agtgctaaaa tcggtcgaaa tttccgaaat
    34561 agcttatgct ttaaaaggtc aaccggatga aatacgcgac ctctttaaaa gcttactacc
    34621 ccaacgttct cagatcattc tggaagatga gatgaagcta ttagaaggac cacaaccacg
    34681 gcgtaaagtc gaagccgctc agaaaaaaat tgtcgaaaaa ttacgcgaac tcgaaaaaga
    34741 aggacgcttc aatctcgaag attttatgga taccgacttt attgaataat agttttaata
    34801 aataccaaaa aagccccgcg ctggggcttt tttaatgcta atttgatagt ctataccatc
    34861 tattttaaaa caactacctt atgaaaatta ttaagacctt attaatcacc ctgctactcg
    34921 gtggttttgc caacctgaaa tctgacacat ttgaacagca ggaagagaga gttcaaatcg
    34981 ctcttaaagc aatgtacaat cgggaataca aacgcgccgc cagtcttttc cacgaagtat
    35041 taaattccac tccctttcat cctatgggtc cactgggagc attagcggtc gaatattatc
    35101 aaaacgaagg attaatcggc tatcaccagc gtaatcaaca atttcttgtg gatattgagc
    35161 aggctttgca aatttatgcg gaccagatta aacaaaaccc atcgcattcg gaatacttct
    35221 ttttctatgg aactgttatg ggcttgcgcg cccgcattca attggcagaa aaaaattact
    35281 tcggcgtgct aactagcggt tataacgcta tccgattcat caaaaagacc gaaaaactct
    35341 atcctgacca accggatttc caattgccgg tcggtctatt taattactat gtcagcatct
    35401 cagcccctta tatgaaaatt gccagtcgcc taatgcgcga atctggctcg cgcagtgaag
    35461 gcctccaagc catcaaagcc gacgctgatt acggcaacta cgggcgctat gaagcccgat
    35521 cgcttctagc atacctctat ttatactttg agtcgaatta tagcggcgct tatgaatatg
    35581 cccgcaaaat ggctgaagat ttccctaaaa atccctatta tgccgcgtta actgccgatg
    35641 ccgcgatcca gattggcaag ctcgatcagg ctaattatta cctaaaacgg attgaggctc
    35701 tgcttccaac cctacctctc aaaacccaaa aggactatcg aggaatactc caccttatct
    35761 atggcagttt ggcgcttgcc caaggtcggc tcggtgaagc cgaatcgaat ttgatcgaat
    35821 tcatcaaatc ctacgattat gaactggatt atgacctcgg caatgctctc gtgagattag
    35881 gtaatgttta cgatctacaa aaacgccgat ccgaggctat cgcctgttat cgcaaagccg
    35941 tccagcttaa taatcgcacg gcggctgtca gcctggctaa ccaatatctg aaacagccat
    36001 ataatagaaa ctaaacccgg tttcctaaat atattatcat ttcacagatc aattttgggt
    36061 ttgattttta actgattcgt aatatgataa ctccgatttt ttctttatat tcattccggc
    36121 tttttaattt ctaaaacaat gtcaactcct ccttgccaaa cgcgctacat tatgaaaata
    36181 atcaaaatga ggttaagaac ttatgaacgg tcaaattccc gtgcttatgg ttaacggcga
    36241 atcgctcgcc gccgcttggg aaaaatcatt agtcgaacta taccaaaaag gttgcgatat
    36301 caaaaccgag tacgacaaac cttctgatcc gcttagcaaa gattgtacta tgatgatcgt
    36361 cgtccaaaac cctttagccg aaccaatgat tcattgtgat ttcccaggtg gattgacaga
    36421 cctgcaagaa tatgttctgg aagtgttgga aggaatcaaa gaccattgca ttcggtcggc
    36481 tgagaatccc gccgatgccc gctgggaata tacttatcac caacgtttat tcaattatca
    36541 ggtgcctggt tccgcaaaag tttacgacca gattgcgatt atggtcgaaa aattaaccaa
    36601 agtccctcat actcgccgcg cccaagcaat cacttggaaa gtatgggaag acaacgattg
    36661 ctacgacccg ccttgtttgc aaagcatttg gtgccgtttg ctgaaagacg ataattcgat
    36721 ctggactctc aatacgaatg tccgctttcg ctctaacgat gcttacaaag ccgcctttat
    36781 gaatatgttt gcgctaaccc agctccaagt cgaaatcgct aatcgaatcg agcaagcaat
    36841 cggtgaaaaa atcaacatcg gtcgctatgt ccatcaagcg gatagctatc acctctacgg
    36901 ttcttatttt gacgaattcc aaaatcgttt cctgaaagcc ctcgagacgc gctcctttga
    36961 ggagcggact tttcgttacg ccgacgtaag aagcattatg acagaagcca tcccccagat
    37021 tcgcgcaaaa gccgctcgta tgggacgtga ataaaactaa accgtttaca aatcacatta
    37081 gattatcgca acctttgcta cattctaagt ccaagcccca attaattgga tgattctcta
    37141 ttttgcaatc atataacttt tataaagtta tgaatgcaac aaatttaccg cgtagatgaa
    37201 ccaacttacg ataacttgtt aatttctata aggtttttta attgttgcca tcttgaagat
    37261 tttagcctaa ggctacatct aaaatcatca taatggtaaa acccagcatc gctcccagag
    37321 tcgcgacatc ggtactaacg tctttctgag attcgggaat taattcttcg actacgacat
    37381 aaatcatcgc ccccgccgca aacgacaggg catagggcaa caaagcctga atcgtcaaaa
    37441 ccagataagc accgataatt ccggaaatga cttccacaaa accggaaagt tgaccgtaaa
    37501 aaaaactctt gccgat
//
LOCUS       DAOSMP010000018        36772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_1171_length_36772_cov_14.877468, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000018 DAOSMP010000000
VERSION     DAOSMP010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 36772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36772
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1171_length_36772_cov_14.877468"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   19..420
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=19..55
                     /rpt_unit_seq="gtttcaatccttggtttagtggataaaggacttagac"
     gene            complement(597..1181)
                     /gene="cas4"
                     /locus_tag="PKX09_04650"
     CDS             complement(597..1181)
                     /gene="cas4"
                     /locus_tag="PKX09_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOU17145.1"
                     /translation="MESLKITPTELLEYYFCPRFIYFMNVLKIPQYEDRRYKVQKGRE
                     VHAERQSHNKDYLWKKVGATARESNVFLISEKYHLRGVVDEVVTMADGSLAPVDYKYA
                     VYPQFVYRSHKTQILSYCLMLEDTYQKPVKCGFIFYIRDGNRQVKVDYTEKTKQKIIN
                     DITALLGIIQNENIPERSENHNQCFDCTYKNICV"
     gene            complement(1233..1538)
                     /gene="cas2"
                     /locus_tag="PKX09_04655"
     CDS             complement(1233..1538)
                     /gene="cas2"
                     /locus_tag="PKX09_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOU17146.1"
                     /translation="MKPTDQLVWVIYDISKNKIRTKVIKATELAGLYRVQKSVFLGTI
                     NHTLLDELVMKVKDLIDPNTDSVYIFPMCEPDFKKVITAGQAFDKKLVTDEVKALLF"
     gene            complement(1525..2511)
                     /gene="cas1"
                     /locus_tag="PKX09_04660"
     CDS             complement(1525..2511)
                     /gene="cas1"
                     /locus_tag="PKX09_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOU17147.1"
                     /translation="MQIILSTFGSSISKKGSMFEVFSQGERHTISPNKVSSILIFTGT
                     NITSDAIELALQSNIDILILNKYGDPIGRFWHARMGSTTRIRRAQLQLVKSAAGIQYG
                     LQWISGKFDNQIKLLTTMRERRTRLSAEITNALNTIQKARESVDKLEGSIEEVRQQLL
                     GIEGSAGKAYWDIFAKFVPEHFAFDGRSRQPAKDEFNAMLNYGYGVLYGVVEKAVVVA
                     GLDPYIGFIHTDNYNKVSLVYDVIEKYRIWAEETILDLFVKKAIRKDLFDKLANGYNL
                     GAEGKKVFLPALNEYLDSPVHYKSRNVRRRDIIQLDLHRFAQELLEKYNETD"
     gene            complement(2592..4985)
                     /gene="cas3"
                     /locus_tag="PKX09_04665"
     CDS             complement(2592..4985)
                     /gene="cas3"
                     /locus_tag="PKX09_04665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOU17148.1"
                     /translation="MSSFSLLSHPNRLLSDHIHGVWKNADFQFDKTSTDFPSFNRLDL
                     KTVLKIACLFHDFGKSTPWFQRYIRNIEGKFSADDRNLRQHGLISAFMVFGILNSIFP
                     TNLFLPALGFMIVRRHHGNLESFQNFFTITDEEFELCYRQIEKIDYEEYRRIVQPWQV
                     DHFVQKDFIEQTLLEIQSGKLRRFKPVKKLDSTFTLDHYFVVNLLYSLLINSDKRDAI
                     FQAPVSISSAILPSSQVESYQKTLSANSSQINAIRNSIFNSTSSSIDHLHNPDMRIFS
                     INTPTGTGKTINALNAALKIREKYNLSHIVYCLPFTSVIDQNFDVFDTIRRFAGLPNE
                     TGILLKHHHLAEICYRSQDMFEREILYNPDEALHLIEGWESHIVVTTFVQFILSLISN
                     DNASLRKFQRFSNAVIILDEVQSISHEYWQVIKESLLKLTQVLNSRIILVTATMPLIF
                     SEEHSEIVELVPSKFQIFNSLARIQLDLADLEQGYITWDLFCERMNLLVNQNASRDIL
                     IVVNTIRCAREIYLYFKGHNITHELVYLSSHIIPRHRIERINYIRQRNRNRPILVIST
                     QLVEAGVDIDLDLVVRDFAPLDSIFQTSGRCNREGHQDYIGTVKIISIKDSQNRKPSE
                     MIYDRFLLEKTRKVLTGKRVIPESDFYLLSQQYYSELSESGSQAISGEILSDMRNLSY
                     EDDNGQPLSNKLKLIDQDYCYSVFVELDEIAEQFWNKYIKVVSSENNIKKRSLLKESY
                     RNLADYIINIPKKCLNRIPEYGIYRLKKSMVNEFYDSITGFNPESLLPEENSTLFFG"
     gene            complement(4972..5679)
                     /gene="cas5b"
                     /locus_tag="PKX09_04670"
     CDS             complement(4972..5679)
                     /gene="cas5b"
                     /locus_tag="PKX09_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5b"
                     /protein_id="HOU17149.1"
                     /translation="MKYLVFDVKADLAQFKKPFTTMSPQTFCIPTGTAIIGMLSAIIG
                     LDKKQYWQFFPENSYHLAVGVCKPIKKVVIPINTLKTTSPMNFSRFEEHKRTTMEFIK
                     DGTFRIWFSWNDAERFQKLSQMVKNHESYYTISLGLAWNLADYDYIGLFDGTSCSDSS
                     KFIEINSIIHKDQVIEIDFNDRVIYSTRIPVRMKTDGSREVEQYSDYIFDNNGRLVRA
                     KVNEYVQLANGDRIVLL"
     gene            complement(5692..6591)
                     /gene="cas7b"
                     /locus_tag="PKX09_04675"
     CDS             complement(5692..6591)
                     /gene="cas7b"
                     /locus_tag="PKX09_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas7/Csh2"
                     /protein_id="HOU17150.1"
                     /translation="MSEIIKNRSELLFCYDVTDANPNGDPLDENKPRIDEETGLNFVT
                     DVRLKRTIRDYFIAYKGFDGTGDKDVFVKEKFVEGDENKGIQDGKLRAKDFDNNKEKI
                     LNTCIDIRLFGGVLPLEKDSITYTGPVQFKIGRSLHKVAMKHIQGTGAFASKAGVKQR
                     TFREEYVLFYSFIAFHGIINQNAAQHTGMTEEDEQLLLEAIWHGTKNLISRSKFGQQP
                     RLLVKIDYQTPNFFIGDLDKKIKLVSSKNDLQIRSIADFKIDITDLNNAILAAGSHIS
                     AIFYQKDPKLEIDIPSNWRLLNL"
     gene            complement(6584..8374)
                     /locus_tag="PKX09_04680"
     CDS             complement(6584..8374)
                     /locus_tag="PKX09_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02556 family CRISPR-associated protein"
                     /protein_id="HOU17151.1"
                     /translation="MLNKVKIIGECVAKNTGNQNILRLNTTLKAGNVIILDFLLDEQN
                     CKFLGVDVMQFDQSKNKLILLKPAPANVKSEFPSYQIAVNKEQEEQKNNIRKSLKKMA
                     NTLENCSLVNTALNPLSNLFQKEFDQIIEIILSKIDPTMSNICTIRINGKLPGESSFF
                     KPIIDHFLEDQSQEYYCKYGGIISKGKNNACYICGLKNPVTFGFCSTYNFYSSNELAY
                     IAGGFRREDSWKNYPVCPTCATDLFIGKEWIQHHFDRYFYGNNYLLLPAPTIDRDDFF
                     PILVDIHKAYTDISLKQSHEKDRQRRLEMEEDIFETLANQKDQVTFTFLFYTVNNSEF
                     KILQEVEEILPSSFQKIIKAKKTVENHADFHNITGLYQKKVSDNLKFNFGIIKTFFDS
                     KFNNDFLDITAKILKNRPVDRQFVLHQIVNVISEKFRQNQLYFEIQKGMIFMKFLYSL
                     KLIENPKNKMEVEMDNKYQDYFLEHPDFYDADWKKAVFLLGVLAQNVMDIQWQERGST
                     PFRDRLNGLKIDYHTVKRLLPECINKLEQYKKNYYRELEEVIAVLLESSEPDLRNQSV
                     DEISFYFAMGMNRNKQFKSKEEINEGDQNE"
     gene            complement(8383..8652)
                     /locus_tag="PKX09_04685"
     CDS             complement(8383..8652)
                     /locus_tag="PKX09_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17152.1"
                     /translation="MIVFVLTVVLQSTNEFVGYIKLLNERTKLGEFVAQVKYVNLFWT
                     ISMTHISNHGNHNTTRHHPAPHDFSHGRINPNRKYISSHEFIHET"
     gene            complement(8663..9100)
                     /locus_tag="PKX09_04690"
     CDS             complement(8663..9100)
                     /locus_tag="PKX09_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014799156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOU17153.1"
                     /translation="MSHALVKIWIHAVFRTKNEAVIIPPDLRPALNQQIKNILTEQNC
                     KVRVVNSVKDHMHCLFLLSADADIAGVIKNLKGIFSHWINHENLTRSKFTWQVGYGAL
                     SVSESSVPNVEKYIGNQERHHQKTTFQEEYDRFMAIYHPELIK"
     gene            complement(9245..9922)
                     /locus_tag="PKX09_04695"
     CDS             complement(9245..9922)
                     /locus_tag="PKX09_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas6"
                     /protein_id="HOU17154.1"
                     /translation="MSRTIIVKIIQVQFPNNPMNNFDIPKLRGFFANRYPKCDLIHNH
                     LADGRFKYGYPSIQFKVLNRIPTLIGLSEGFDILKEVFQDVYELVIAGEKAKIWEKSI
                     QIREDNFGQIDSFFDYQFTSPWMALKEENYETYKTLDKIEKQQFLRHLLRENLKTVSK
                     GFHYNIPNIESVKVEGYFKPRPMNFKNIRMVCFTGEFTTNFLLPDYLGIGKQVARGFG
                     TVKRVSR"
     gene            complement(10569..12989)
                     /locus_tag="PKX09_04700"
     CDS             complement(10569..12989)
                     /locus_tag="PKX09_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU17155.1"
                     /translation="MAQPPASAPRPTSGRLSGTVLIKNTDKPIEYAAITLHGLQDSSI
                     ITGTITNEDGIFILDKIPFGRYFAKISFIGYKTAVINNIAITPPKPEVVLGTVYLEPV
                     AIDLSSVTVTGEKKALAYNLDKKVITIDNSVGTIGGTATDIMQTIPSVTVDIDGNVSL
                     RGSSSVTLLVDSRPSQIISLDEIPASMVERVEVITNPSARYDPEGMSGIINIVLKKKR
                     SPGYNGMLLVNAGTGGKYNGSINLNYRYNKFNLFANYDMRMGHRLGTGETIRESIRND
                     STFYLNQYQNSENRNRFHNIKLGGDYFINDYNTISLSMLYNLRSFSNQNNTEYLNTDY
                     NAVGYNQFTRLSYGDTDNQGQEYGLFYKKTFPQPQREWTIDLFYSTFSRNSDQSITQQ
                     AVLTDSLITVLPDLENIFSDDANRVITARTDYVHPIGEHRRIEAGYRGTFRHNDSDYR
                     RFNYNYTMTDWFPDSLASNHYIYDANIHAVYGIYSRELGRLQSQLGLRIEQFNTNNNL
                     VTIDSSFQKNYFSMFPTIHFRYALTENQGLQVSYSRRVHRPHTWQVSPFVDYSDPYNL
                     SSGNPKLKPEYIDVAEIEHSYENRKTSINTSLFYRQVNGMITRLTELQEGGITFTTYQ
                     NLNNGVSYGIESVISHNLTPWWRINTNISYFNNRLYGTGISIADANESHSWNARVNSF
                     FRLGENTEMQIMLFYFSPTVGVSGGGRGFHGPGQGRMQENYFVNLGAKHNFLDGKMSA
                     FVRVSDIFKTQKHGQTIYGDNFKTTSLRHPESQVIFFGISYKINEGIKERQKRTDEEN
                     EYPDMEEM"
     gene            complement(13236..14153)
                     /locus_tag="PKX09_04705"
     CDS             complement(13236..14153)
                     /locus_tag="PKX09_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOU17156.1"
                     /translation="MLNRYKILDKRIVLNGGEDASILVFINPDEAEKRMLVDQFMIDE
                     HTLNSALDPDELSRLEYEPNHIAFIFKRPRSITAKENILFKVTSTGVFLFKDQIIIVA
                     SEDIPIFEGKIFNRVNSNQDILLKLILRAIYHFLEHLKAINMVTDEFQEKINTSLENK
                     YLIDLFSLAKSLVYYLNAINSNGVLLEKLKINTVKIGFSQEECELLDDIIIENTQCVK
                     QAEIQSNVLASLMDARASIVSNNLNVLMKTLTIITIGIMVPTLIVSIFSMNVDLPLPR
                     NHPSSFWIIIGMALVSVVAFFFFWKRKKW"
     gene            complement(14342..15739)
                     /locus_tag="PKX09_04710"
     CDS             complement(14342..15739)
                     /locus_tag="PKX09_04710"
                     /inference="COORDINATES: protein motif:HMM:NF015573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HOU17157.1"
                     /translation="MTPWDFTLNFKDMILDFTALGAFLVLGTIARRYIPFFQKYLVPN
                     NIIGGFLGLLLSQQVLGLIYLDENRLGQYVYHLLALTFIVIGLGKSKTKFGKGPVSKC
                     FTELTCYIVQGAIGLLVVLALIYTVSPDLPAAMGLLVPLGFGMGPGIAYTMGHSWEKF
                     GFEGGGMIGLTFAAIGFLIAYFAGVAIVNRGIKNRETTLIKGSESINNDIRTGVVKEN
                     KPEVAGWLTLSPEAIEPMAFQLGLISIVYAMTYGLIYFVTKLMTTYGLKDFVDTIWSF
                     HFVIALLIALLVRKCLDITKKSYVIDNGLMNRISGVAVDFLVTGSIVAINIVIVGKYW
                     LPIAVMSILAGFATYFVIRYTSRRAFDDFHFERMVGIFGEMTGTINSGLVLIRITDPE
                     FQTPSAEDLALGGGLALFLGFPLLILLNAPMNFWHNSLGGYWLTLALMLVYLVILWVV
                     WRLVGYIHFKLPENK"
     gene            15976..16683
                     /locus_tag="PKX09_04715"
     CDS             15976..16683
                     /locus_tag="PKX09_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17158.1"
                     /translation="MKYYSPFLIIFIFLTALLSPAAEVEKYQGKIDPGNGQIYTCRGE
                     IIRNDGKVTKTSYFYDENGKILLNEKVVFDEKGYKLISLVSEEPRYGRIQEIQRKGSQ
                     YIARYCKNSGEEFREHIINDQPLLLHGSNLPVYLSQNLDLIGENGHICRLIIVPMKME
                     IEMIFKNKGIKNIDGHQCYEIQMDAANLFLKPLVKTHYFYYGTEEPHYLYHYIGTISL
                     TDPRGNDIWGTISFTYE"
     gene            complement(16713..17261)
                     /gene="folE"
                     /locus_tag="PKX09_04720"
     CDS             complement(16713..17261)
                     /gene="folE"
                     /locus_tag="PKX09_04720"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOU17159.1"
                     /translation="METEEIIRNLLVNIGENPNRPELLKTPARVAKFWQHVSQGYKVD
                     VLEMLKESLVYEDYNQMIIVKDLDFFSLCEHHLVPFFGKAHVAYIPNGKIIGFSKIPR
                     VVTAFSQRLQIQERLTQQIADTLNEALEPKGVGVIIEARHLCMQMRGVEKQNSNITTS
                     VMLGELHDEPQARAEFLKLISR"
     gene            complement(17344..17835)
                     /locus_tag="PKX09_04725"
     CDS             complement(17344..17835)
                     /locus_tag="PKX09_04725"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HOU17160.1"
                     /translation="MNNLSQVSDSIFNNIRSILEDARSRAYRSVNFIMVEVYWNIGRI
                     IVDEEQKGKRRAEFGKQLIADLSRKLTVAYGRGFDPSNLWYMRKFYLLFPILDAVRRE
                     LSDSLANGDSKSVIRLGNSIRPELTWTHYRLLLRIENHQARSFYWNEAAYNGWSTREL
                     DQW"
     gene            complement(17832..18473)
                     /gene="nth"
                     /locus_tag="PKX09_04730"
     CDS             complement(17832..18473)
                     /gene="nth"
                     /locus_tag="PKX09_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015219426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOU17161.1"
                     /translation="MITNKSEIAPEIFSRLSAAYPDARCALNHTNPRELLIATILSAQ
                     CTDKRVNMVTPALFQKYPTAQAFADADLEELKNDIRSTGFYNNKAKAIKSAMKSVVEK
                     FNGEVPGTMEELLQLEGVGRKTANVVLGDAFGVPAIVVDTHVKRLANLLGLTKNSNPE
                     KIEQDLMRQFPQEQWTKLGHLLIEHGRQVCIARRPQCSKCVLNDICPSARNNK"
     gene            complement(18536..18676)
                     /locus_tag="PKX09_04735"
     CDS             complement(18536..18676)
                     /locus_tag="PKX09_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17162.1"
                     /translation="MRSDKIIYSINIADVQTVAEQELDRKLTQEELKFIADEALSTDY
                     FG"
     gene            complement(18666..19712)
                     /locus_tag="PKX09_04740"
     CDS             complement(18666..19712)
                     /locus_tag="PKX09_04740"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TdeIII family type II restriction endonuclease"
                     /protein_id="HOU17163.1"
                     /translation="MTNAKIEKIAFEVIKTLKSRFDTFPENEMDNRNAPFHEAFLNAF
                     SVKLEGKVKSVPFLITLSSWLHGLSTTLGQSFFERVSHILSNGEKREFRDLIISSRQE
                     TVISDIITELKNGQRQPDVSSEDNILKYQGEILDRHIPNFTADVFYETDNEIIAIELK
                     TVKPNSGVFKVEKEKILQAKTGLRNKYLGKKVSYYLGFPFDPTSKQPTKSNKERYFGY
                     SIDFRKYFHPQEILLADELWNFLSGETGTMELILSIINSIAKPDFMNKFDIINQPILQ
                     IATKSKAILHDWFLFTELQIIQNFPHLNDLSIKDKTLKRILAQCPFSPDGKINTNRLD
                     IIRPFLEGGEQNAK"
     gene            complement(19709..21319)
                     /locus_tag="PKX09_04745"
     CDS             complement(19709..21319)
                     /locus_tag="PKX09_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOU17164.1"
                     /translation="MGEMETLFKNQNNILSLKDASRWASDYLNKKVTESNITYLINYG
                     KINKTSNNGSIYVDISELKNYYDSFWGKREINWKNQLGEDLNWHLSFDFLKEADTTKH
                     VHRLHPYKGKFIPQLVEYFLDDHIDEFKTEVFFNKGDIILDPFCGSGTTLVQANELGM
                     HAIGVDVSAFNALISNVKVKKYDLFSLQSEITKITRSLRVFNSKRNILQFDVELTEKL
                     NEFNNRYFPSPEYKQKVRRKEINEDNYGKTKSDEFLPIYWDLVKKYGVKLKQDTENNF
                     IEKWYLQPIREEMDFVFEQIKLIQDSEIKKIISVIFSRTIRSCRATTHADLVTLIEPV
                     ATPYYCAKHGKICKPLFTILNWWERYSKDTLVRLATFNKLRTNTFQYCLVGDSRNIDI
                     IEKLQSKNKELSDLVQRKKIDGIFSSPPYVGLINYHEQHAYAYDLFGFERNDELEIGP
                     LFKGKGIEARESYVQGITDVLINCKRYLKPNYSIFLVANDKYNLYPMIAEKSGMKIVN
                     CYKRPVLNRSEKDKGAYSEIIFHLKDVQ"
     gene            complement(21394..22419)
                     /locus_tag="PKX09_04750"
     CDS             complement(21394..22419)
                     /locus_tag="PKX09_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOU17165.1"
                     /translation="MFEKYYNQIIQGDCIELFKNIPDNSVDVTFADPPFNLGKKYNGY
                     RDNKEVQAYLDWCKRWIFEMVRITKPAGSIFVHNIPKWLTYYASFLNEIAYFKHWIAW
                     DAPTAPMGQSLQPSHYGILYYAKDLKKNKFFEIRYPHKRCRKCGYLLKDYGGKKAGLH
                     PFGPLVSDVWTDIHRLKHNKYRDEHPCQLPVHLLERIILMSSDEGDIVLDPFVGTGTT
                     AIAAKRLGRKYIGFDIDENYITITQKKLVQEELCSKINGIWLSFFLGEPITIRDKDWV
                     ELTKYFVIPDNIKEIEYSKIKLHPNIFIPETVIKSDLSLFENTESEEEVYKHDLCKVC
                     QSQNIGK"
     gene            complement(22412..23401)
                     /locus_tag="PKX09_04755"
     CDS             complement(22412..23401)
                     /locus_tag="PKX09_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009413428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17166.1"
                     /translation="MKTIDEKYRTVIENNTYYYFDEEFESAYESHIFSVKESIIHLKN
                     EVQNNGCKKEVFENFLRNKKELGLKALLAITGFSNESLLRLITIIRIVDDKELNDFTF
                     MNYWINNKRTTKDNEISEWNTSKIEQLVRENEYFRKCLVNIFYEGSSIDFFKRTLPPF
                     EFQKLSLEKLNFDINAIIDTLARYKEKGSRAAKGKNNPEILIERILKKRKIPFEKGDL
                     SELIEYAPEQKRTMDFIIPNKQSPKIIIESTFLVTTSSGQGDKSKTEISIDSLIKEHY
                     PDARFLGFVDGIGWYVRKRDLKRMVGAYEDVFTFHQEELNRSEKLLCEVMKNV"
     gene            complement(23404..24669)
                     /locus_tag="PKX09_04760"
     CDS             complement(23404..24669)
                     /locus_tag="PKX09_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOU17167.1"
                     /translation="MKVLGNYSFYELPACIETLFDKNVTLLPSVTELFELELAYLEYK
                     SLPKDQIIERSAYIQSIDGKFTTHYLMYNQTCEGLIENRSNVTKNYFENGTFSTGYAT
                     HGLFPYRGKFHPQLIKALLNIIGVEKGQTILEPMCGSGTANIEAALMGINSYAVDISP
                     FCQFMTQAKYDALQITNSSLSLLIENRDKLFNFFSQGNILKKLKNITDNEKLAIYNLA
                     LLAYLDSMGYSKRVIKSDHRQLFEKVLTRYENTVRNFINNSYFKKVQLGKLNVLSDSD
                     ALDLKIVDNSIDGVITSPPYSFAIDYAKNDEAQLEYLGYNIEKIKGKMIGLIGKNKDE
                     RLNNYFRDMKKVCSEIYRVLKNDKYFVMIIGSNTNQTGGIRLEKTIIQNCEEIGLKLV
                     KSILKPIKGMRNTMKDEYLLFFQKNPDRN"
     gene            complement(24806..25732)
                     /locus_tag="PKX09_04765"
     CDS             complement(24806..25732)
                     /locus_tag="PKX09_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOU17168.1"
                     /translation="MIPFPLDERYYRYGRYLRERFQTRVYRITLDAGFTCPTRDGTKG
                     TAGCLYCNNESFAPKRTEELPSLSDQIQKGIASARRFHQADKFLAYFQPFTNTYAPVA
                     KLERLYHEVLAFPEIVGLCIGTRPDCVSEEILQLLEHLNHETYISIEFGIESVYDKTL
                     DWAQRGHDFAQTRWAIQAAKAHGLHVAGHLILGFPTETRTDILATPGVLNSLGIDALK
                     IHHLHVVKNTPLAEFYAENPFSLFTEKEWITLVVDFLEHLHPEIVIQRLCGDAKPGTL
                     IAPQWNLTKNEILTGIRDELARRGTYQGFLVE"
     gene            complement(25789..27771)
                     /locus_tag="PKX09_04770"
     CDS             complement(25789..27771)
                     /locus_tag="PKX09_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOU17169.1"
                     /translation="MAPTNTPLGSALFIVLLVVALGWFAWLMYRRIAVIAAGQPQNRF
                     DHIGRRIGAFFTQGIAQTKIRDRRFWSAGYMHTFIFWGFLVVSINTIQMIGQGFNPNF
                     VLPGFHTGQTLGQIYLWLKDVFEVLVLLSVIYALIQRLIIRPKRLTLSASANLILLMI
                     AILMVTDLLMTGLTVAFEADADRTVVGVAFARIFCNTAPEFAVKFFHLNWWLHLITLL
                     SFFPFLPLSKHFHIITSLPAVFFKRLDSGYVRFLDVENGERFGASRIEHLGWKDLLDI
                     FTCTECGRCQTVCPAFESGKPLSPKEFNIALRHHLIKYTTDIIKDKRQKSQSPRAEKL
                     VGDTIGEETLWACTTCRACEEVCPLSIEFIDRIVEMRRHLVLEESRFPEAAELAFRNL
                     ESYGNPWGASDREKWTEGLAIPRAEEKADFEYLFWVGCAGAFDEHAQKTSRAMIKLLN
                     AARVNYAILGNAETCTGDAARRLGNEYLFQTLANQNIETFHNLGVQKIITQCAHCYNT
                     LKNEYPQLGGKFEVISHVEMIASLLAEGRLKINQPIKGKLTYHDACYLGRHNGIYDAP
                     RQIIAALQADFQEATRHGSNALCCGAGGGRMWLEEAPAQRVNTLRAKELTKLNPDIIA
                     TACPFCTTMLYDGLKEQNSSVKCLDLAVLVASQLAE"
     gene            complement(28245..29969)
                     /locus_tag="PKX09_04775"
     CDS             complement(28245..29969)
                     /locus_tag="PKX09_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOU17170.1"
                     /translation="MKNLTIITVLIILITGNAFAQNWNAVCVGINNYGDPENNLHWCV
                     KDAIEMKEYLITYKQWSSGKITRLLDNDASESVIHTAIQNMPRSAGNTNLFSFSGHGN
                     SRELDTLYGGSDGDGLYAPDWAMITPSELQADLGSTFNQSTAFLDACCSGIFPRVMTK
                     GVISSACQADELAEEDYSDGSLGLEHGFFSYYLLDGLAKSSITTAEQLHNYAAPLTTA
                     LSNSVDPNYPMHPQLGDRFSGLLSIYNSSYTLSGVLPRNQHWSAPSALQGNVYVPSGV
                     TLTLCADLVLNQNGYTIYSGGGTIIDQRPFGTISSNTTWDENKVLKGNVNVPSGVVLT
                     IVSGVMVNFNGYSLTSTGGTIAIQSGGIITLNPATYVDITGNGSTYEVNGINVGASFN
                     GIGQSIKAIPPSGFGVAGWSDGVGGNYREINGNITVNARLKALHKSNTIAAWDNTSQR
                     KLIQTLAGGNPRWLHQVYTSAGHVWLEHSGDGGSTWTFGNNGQPLDGTAGGKCPSIAF
                     TTHNQGYVVDNYIGVVWQEKYGTKYKIKGKIFNQYGDVNSAPSPYYEVTTLFTEPNDT
                     YNAVNNRL"
     gene            complement(29966..30583)
                     /locus_tag="PKX09_04780"
     CDS             complement(29966..30583)
                     /locus_tag="PKX09_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17171.1"
                     /translation="MENKIMRNRHFKISFLLIVFFLTVSLLQAQDATKQLVVTEVYLD
                     STNREVWIEIFNPSDETLSLNSMRISGIRRPSVLPREYEGQKSIELKPGERMIVCSDI
                     QSFRKKHGTRIRAADLKIMEKLLGGGFIAINNLTDTGSKNNVVRIGASGKSRGLSAIV
                     GDDQVLNITEDGKSYARMIQNNKEISVWFKAIPRPGQAKERGNEQ"
     gene            complement(30773..32128)
                     /locus_tag="PKX09_04785"
     CDS             complement(30773..32128)
                     /locus_tag="PKX09_04785"
                     /inference="COORDINATES: protein motif:HMM:NF024209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOU17172.1"
                     /translation="MKKIFIYTEATMNYIKIMPLFILFFISCNEIVNNESEKEGADAG
                     EDQTTYIGSYVVIDISNSMLPEGEAINLIEWTQDAGNPQEIYLFAQSSLNDKWIVSFE
                     KEGRYKYTLNIECNSGNVFTDDVVITVNPRQPSVIEDIYLEARIRYELSYKEGELNAG
                     KLQMLDSLPASDVVMKNYKTKSIQGIENCTNLIYLALGLQSITDLAPLADLTKLEYLD
                     LSQNRTIEDISPLTNLVNLKKLILDTNPIKDISALGNLTNLKELWLWFTPITNLNPLS
                     SLSNLEILHLSGVGEGITFESIEPLRNLLNLKQLDLSGGNITDITPLENLTELILLDV
                     GYNNLTEISPVSKMKKLIRLYIRLNKINDISGIKNLESLDYLDAADNEIKDISELQYL
                     PNIHLIGLSRNQIEDILPLVNNANLGKGVYLYLDGNPLNEKSVNEYIPALQARGVSVF
                     Y"
     gene            complement(32146..33099)
                     /locus_tag="PKX09_04790"
     CDS             complement(32146..33099)
                     /locus_tag="PKX09_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17173.1"
                     /translation="MNPEERKTVGFFISNSEGKDCRKGMDLSQDSDFQRNNIPNNRNK
                     SNNGNFRGSGRTLFKVYKSENYEGLLSLTISLFFSNIKLFARQGGLNLFFVFKDMRIN
                     CRRFSMKPIRVLLLIMILEIFSFGADIKYFNPGFKVGMNFTKQSFTHLEPIKYLYPLG
                     FDMGLSNTITFNTHFFLLNDFYFSYTNSMVKYSPAAAVSPCYANYKNDFLRLSNNLGL
                     NIFDKLGILLGIDIGYLIKSNLTIYDREIGNIGFKKNMKGSFPDFDFILDIGIIKKFN
                     IRRNTYSFEVKLLKGLTQYHDDIINIDFTNYYLQSNIGIVF"
     gene            complement(33181..34572)
                     /locus_tag="PKX09_04795"
     CDS             complement(33181..34572)
                     /locus_tag="PKX09_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17174.1"
                     /translation="MYYGGGGWGYGVVTSGSSTNCLIENNIFRYLRHAMIAGGGSNCN
                     VWTFNYSREQYSTWWLGIGYDDRDLDLHAKYPYGHLFEHNIVERIASDDYHGDNGPYN
                     TFIRNMATGGIALIKTMHEWATLGNMEKQGDLLYPLRFNWTGDAVPATDLYGFLNNYT
                     TPVPHNVAYDFGAYSSYRLDDVSYYYSSKPEFLDGYSYPAIGPKTRTGGDLSFSIPAK
                     GRYNGSGVKTYLKNPTPKPFTTSGALSYDQTWFGIVALTGHVTVPNGVTLTINSGATV
                     NYNGYSLTSTGGTISIQSGGIITLNPASYIDISGTGSTYEVNGINVGASFNGIGQSIK
                     AIPPSGYGVAGWSDGVGGNYREINGNVTVNAKLKALHKSNTIAAWDNTSQRKLIQTSA
                     GGNPRWLHQIYTSAGHVWLEHSSDGGSTWTLGNNGQPLDGTAGGKCPSIAFTTHNQGY
                     VVDNYIGIVWQEK"
     gene            complement(34627..35493)
                     /locus_tag="PKX09_04800"
     CDS             complement(34627..35493)
                     /locus_tag="PKX09_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOU17175.1"
                     /translation="MKTNHLIMIVFGFLLILIAGIKSQTLYNGVGHIPAYYQETWNVA
                     GLLQNMSSVEPKLVIPISPGAASSQVSTAIGTARDHINRTGGLAIIYFPEGTFNFTST
                     ITLTQNDRNIVFQGAGSDKTTLVFQNMKNSPCFALTGTTGDWIDLDQDFNKGESVIHA
                     APVGVLSGILVGDWIHFIQRNFDYHTERDFEREIVGQITRISAQGSDATGDWKEIKDE
                     ANMDYEDAVNTDYSLKVCKVTPIQNIGIENLKIIRSPNNKPSNNPYNIQLFYAINCWI
                     KGVESYKAACNH"
     gene            complement(35534..36202)
                     /locus_tag="PKX09_04805"
     CDS             complement(35534..36202)
                     /locus_tag="PKX09_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17176.1"
                     /translation="MKQIIPLLIIIFILPISVKAKSLTYIKIGANFSSFRKEEGRNEP
                     GLCFGAGKEFYPIRSFNGFFDLGIDFKRKKYILENRTWQSDPYYNISDIEMGDINVNI
                     LYAELPINVGYSIVINNQFVSNIYTGYSLSIPIKNHTKISDKKTIPLGPDERKTYNFD
                     YIPLDENYTIPSTNFILGARLSFKRLAIITSYARALSVTEGVTSLSVRDKIDTFEISM
                     AFLF"
     gene            complement(36252..>36772)
                     /locus_tag="PKX09_04810"
     CDS             complement(36252..>36772)
                     /locus_tag="PKX09_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOU17177.1"
                     /translation="NTYKTGFSIPANGGNLSTSAQQHSSYSVLFKRSSSIYFLHCKGI
                     RYPESSDIQYLRDIEPLYANCKLIGDKAYLDAEYQLELFQIKGIQLETPMRNNQKNYK
                     KQPSIYRRVRKRIETIFSQFVDQFLIRRNYAKTFIGLLTRILAKITTFTLLQFINHLN
                     NKPLNHVKHTLA"
BASE COUNT        10853 a         7200 c         6918 g        11801 t
ORIGIN      
        1 ttttcctctg taacttccgt ttcaatcctt ggtttagtgg ataaaggact tagacagctt
       61 attacagttg tgaatatttc tgaaaatcgg agtttcaatc cttggtttag tggataaagg
      121 acttagacct gccaatgtgc attcattgcg atcgtggcgc aagagtttca atccttggtt
      181 tagtggataa aggacttaga caaaatcaag gcgaaggata ctccgtctta tcttttgttt
      241 caatccttgg tttagtggat aaaggactta gacatgtgta gaacgttcat acaaaaatct
      301 ttttcaaagt ttcaatcctt ggtttagtgg ataaaggact tagacttcta ttgccatttc
      361 tatttgctca ttgcggtcag atggtttcaa tccttggttt agtggataaa ggacttagac
      421 agcataaccg atgctaacta cagaatattc aggaaaatat attttctgaa atctgtaata
      481 atacatcaat attttctgtt gcataatcat caatttttct caaataacgt tagattttac
      541 gcaaagtcgt tctatttaac aacttgtttt ttatttcact atgtcaaaga gccgctttaa
      601 acacaaatat ttttgtaagt gcagtcaaag cattgattat gattttcact cctttccgga
      661 atattttcat tctggatgat tccgaggagt gccgtgatat cattaatgat tttctgttta
      721 gttttttcag tataatcaac tttgacctga cgattgccgt ctcgaatata gaaaatgaat
      781 ccgcatttga ccggtttttg ataagtatct tccagcatta ggcagtaact caaaatttga
      841 gttttatggg aacgatagac aaattgcgga taaacggcat atttataatc aactggcgcc
      901 agtgaaccat cagccatagt tacgacctcg tccacaacgc ctcgcaggtg atatttttcg
      961 ctaatcagga aaacgttcga ttcgcgtgca gtggcgccga cttttttcca taggtaatct
     1021 ttattatgag attgacgttc ggcatgaact tctcgacctt tctgaacttt atagcgtcgg
     1081 tcttcgtatt gcgggatctt tagtacgttc ataaaataaa tgaaccgcgg gcagaagtaa
     1141 tattccaata attcagtcgg agtaatcttc agggattcca taaaaagtgc cgctatctca
     1201 tactacacga attaattcgt ggactagggt ggttaaaata ataaggcttt gacttcatcg
     1261 gtaaccaatt tcttatcgaa tgcctgtccg gcggtgatga ctttcttgaa atccggttcg
     1321 cacattggga aaatatagac actgtcagtg ttcggatcta tcaagtcttt gaccttcata
     1381 actaattcgt ccagcagagt gtgattgatc gtaccgagaa atactgactt ctggacgcgg
     1441 taaaggcctg ctaattcagt agcttttata accttcgtcc ggattttatt cttggaaata
     1501 tcataaatta cccatactaa ttgatcagtc ggtttcattg tatttctcca gtaattcctg
     1561 cgcaaaccgg tgcagatcga gttgaattat atctcgccgc cgcacattac gcgatttata
     1621 atgaacaggc gagtctaaat attcattcag agccggtaga aaaacttttt taccttccgc
     1681 tccgagattg tagccattgg ctagtttatc aaataaatcc ttgcgaatag cttttttgac
     1741 aaataaatcc agaatagttt cctcagccca gatgcgatat ttctcgataa catcatagac
     1801 cagcgagact ttgttgtaat tatcggtatg aatgaaaccg atatagggat cgaggccagc
     1861 aacgactacg gctttctcga ctactccgta aaggacacca tagccgtaat tcagcatggc
     1921 attgaactca tcttttgccg gttgccgact gcggccatca aaggcaaaat gctccggaac
     1981 aaatttcgca aatatgtccc agtaggcttt tccggctgaa ccttcgatgc cgagcaattg
     2041 ctgacgcact tcttcgatcg aaccttccag tttgtcaacg ctttctcttg ctttctgaat
     2101 agtgttcagg gcgttggtaa tttcggccga gaggcgggtg cgacgttccc gcatagtagt
     2161 cagtaacttt atctggttat cgaatttgcc ggaaatccat tgcagaccat actgaattcc
     2221 tgcggctgat tttaccagtt gtaattgtgc ccgtcgaatg cgggtagtcg agcccatccg
     2281 agcatgccag aatcggccga ttgggtcgcc gtatttattc agaatgagaa tgtcaatatt
     2341 ggattgtaaa gccagttcga tggcatccga agtaatgtta gttccggtaa agatcaggat
     2401 cgaagagact ttattgggtg agatggtatg gcgttcgcct tgcgaaaaga cctcgaacat
     2461 cgagcctttt ttggaaatgg atgaaccgaa ggtggaaagg atgatttgca ttttatattt
     2521 ccagagccat gaattctttc gtgggctgac ggataatcct ttgtctatcg gtatttttat
     2581 ccattgatta cttatccaaa gaaaagtgta ctattttctt ccggcaacag actctcagga
     2641 ttaaaaccgg tgatagaatc ataaaactca tttaccattg attttttaag ccgataaata
     2701 ccatattccg gtatgcggtt taagcatttt ttgggaatat tgatgatata atcagccaaa
     2761 ttacggtatg attcttttaa taatgatctt tttttgatat tgttttcgct tgaaacaact
     2821 tttatatatt tattccagaa ttgctcggca atttcatcta attctacaaa aactgagtag
     2881 caataatcct gatcaatcaa ttttaattta ttcgaaagag gttggccatt atcatcttca
     2941 taggataaat ttctcatatc tgaaagtatt tcacctgaaa ttgcttgaga gccggattcc
     3001 gaaagttcgg aataatattg ttgtgataag agataaaaat cggattcggg aattactctt
     3061 ttaccggtta atacttttct ggttttttca agaaggaatc ggtcgtaaat catctcactt
     3121 ggtttacggt tctgggagtc tttaatagag atgattttta cggttccgat ataatcttga
     3181 tgtccctcgc gattacaccg accactagtt tggaaaatac tatccaaagg ggcaaaatct
     3241 cgaacaacca aatctaagtc aatatctact cctgcttcaa ctaattgggt tgatattacc
     3301 agtattggtc tattacgatt tcgttggcga atgtaattaa ttctctctat cctatgacgc
     3361 ggaatgatat gtgatgataa atagactaat tcatgtgtta tattatgacc tttgaaataa
     3421 agatagattt ctcgagcgca ccgaatggta tttaccacga ttagtatatc acggctagcg
     3481 ttttgattta caagcaggtt cattctttca caaaataagt cccaagttat atatccctgt
     3541 tcgaggtcag ctagatcaag ctggattcga gcgagactat taaagatttg aaattttgaa
     3601 ggaaccagct cgacaatctc agaatgttct tcggagaaaa tcaaaggcat tgtcgctgtg
     3661 actaaaatga ttcgtgaatt taaaacttgg gtcagtttca ataacgattc tttaattact
     3721 tgccaatatt catgggaaat actctgcact tcgtctaaaa taatcacggc atttgagaag
     3781 cgctgaaatt ttcgaagact ggcattatca ttacttatta gggaaagaat aaattgaaca
     3841 aatgttgtaa ctacaatatg actttcccag ccttcgatca gatgcagggc ttcatccgga
     3901 ttatataaaa tttctcgctc aaacatatct tgtgagcgat agcaaatttc tgccagatga
     3961 tggtgtttaa gtaaaatgcc ggtttcattc ggtaatccgg caaaacgccg gattgtatca
     4021 aaaacatcaa aattttgatc tatcactgag gtgaaaggta aacagtaaac gatgtgtgat
     4081 aaattgtatt tttctctgat tttgagggct gcattcaagg cgttgattgt cttacccgtg
     4141 ccagtaggtg tattaatcga aaaaattcgc atatctggat tgtgaagatg atcaattgaa
     4201 gaagaagtgc tattaaaaat agaattacga atggcgttaa tttgactgga attagcactc
     4261 agcgtttttt gataagattc aacctggctg gaaggtagaa tcgcagagga tatactaacg
     4321 ggagcctgaa aaatagcgtc gcgtttatca gagtttataa gtaaagaata aagcaggtta
     4381 actacaaagt aatgatcaag cgtaaaggtt gaatcaagtt tcttgacggg tttaaacctg
     4441 cgaagtttac cggattggat ttctaatagg gtctgttcta taaaatcttt ctggacgaag
     4501 tgatcaacct gccatggttg gacaatgcgt cggtattctt cataatcaat tttttcgatc
     4561 tgccgataac ataattcaaa ctcttcatct gtaatggtaa aaaaattctg aaacgattct
     4621 aaattgccgt ggtgtcttcg aacaatcata aaaccaagtg cgggaaggaa taaatttgtc
     4681 ggaaaaatgc tgtttaaaat accaaaaacc ataaaagctg atatcaatcc gtgttgccgt
     4741 aaattgcgat catcggcgga aaattttccc tcaatatttc gaatatagcg ctgaaaccaa
     4801 ggggtacttt taccaaaatc gtggaataaa caggcaattt ttaaaactgt tttcaaatcg
     4861 agccgattga aagatggaaa atcagttgat gtcttatcaa attgaaaatc tgcatttttc
     4921 cagacaccat gaatatggtc ggataataaa cgattcggat gtgataataa actaaaggag
     4981 gacaattcta tctccgttcg ccagttgaac gtactcatta acctttgccc gaactaaccg
     5041 accgttattg tcgaaaatat aatcactata ttgttcaact tcgcgactac cgtcggtttt
     5101 catccgaacc ggaatgcggg ttgaatatat tactcggtca ttaaagtcta tctcgattac
     5161 ctgatctttg tgaattatag aattgatttc aataaatttc gaactgtctg agcaagaagt
     5221 accatcaaaa agaccaatat aatcgtagtc ggcaagattc caggctaatc ccaaagaaat
     5281 tgtatagtag gactcgtgat ttttaaccat ttgagataat ttctgaaatc gttccgcatc
     5341 attccagctg aaccagatac ggaaagtacc atcttttata aattccattg tcgtccgctt
     5401 atgttcttca aatcgggaga aattcatcgg gctggtagtt tttagggtat taattgggat
     5461 aacgactttt ttaattggtt tgcagacacc gacagccaga tggtaagaat tttctgggaa
     5521 aaattgccaa tattgttttt tatccagacc tatgatagcg gataacatac caataattgc
     5581 tgtaccggtt ggaatacaaa aagtttgcgg cgacatagta gtaaatggtt ttttgaactg
     5641 agcaagatca gccttaacgt caaataccag atatttcatt tagcctcctg tttaaagatt
     5701 caaaagcctc cagttacttg gtatgtctat ttcaagtttt ggatctttct ggtaaaagat
     5761 agcgctaatg tgactaccag ccgccagaat agcattgttt aaatcagtta tatctatttt
     5821 aaagtcggca atagatcgaa tctgaaggtc gtttttggaa ctgactaatt tgatcttttt
     5881 gtccaaatcg ccgatgaaaa aattcggggt ttgatagtct atttttacca agagacgcgg
     5941 ctgctgacca aatttactac ggctgattag atttttggtg ccatgccaga tggcttcgag
     6001 taacagttgc tcatcttctt cagtcatacc agtatgctga gcggcgtttt ggttaatgat
     6061 gccatgaaaa gcaataaatg aatagaagag gacgtactcc tccctaaagg tgcgctgttt
     6121 aacccctgct ttacttgcaa aagcccctgt tccttggata tgtttcattg cgaccttatg
     6181 taatgagcgt cctatcttga attgaacagg accggtatat gttatagaat ccttctccaa
     6241 agggagtacc cccccgaaaa gtcggatgtc tatacaagta tttaaaattt tctccttgtt
     6301 attatcaaag tctttcgcgc ggagtttacc atcttggata cctttattct catctccttc
     6361 aacaaatttt tctttgacaa aaacgtcttt atcacctgta ccatcaaaac ctttatatgc
     6421 aatgaaataa tctctgatag tacgcttaag gcggacatca gtgacaaagt ttaaaccggt
     6481 ttcttcgtca atacggggtt tgttttcgtc aagcggatcg ccattgggat tggcatcagt
     6541 tacatcatag caaaaaagta attcactgcg gtttttaata atttcactca ttttgatctc
     6601 cttcattaat ttcttctttt gatttgaatt gtttattacg gttcattccc attgcgaaat
     6661 aaaaactaat ttcatccaca ctttgattgc gtagatcagg ttcacttgat tccagtagaa
     6721 cagcgataac ttcttcgagt tctcgatagt aattcttttt atactgttcc agcttattga
     6781 tgcattccgg gagcaggcgt ttgactgtgt ggtagtcaat cttaagccca ttcaagcgat
     6841 cgcggaaagg agttgagccc ctttcctgcc attgaatatc catgacattc tgggctagta
     6901 cccctagcag aaagaccgct tttttccaat cggcgtcata gaagtcagga tgttcaagga
     6961 aataatcctg atacttattg tccatttcta cctccatttt atttttagga ttttcaatta
     7021 gtttaagaga atatagaaat ttcataaaga tcataccttt ctggatttca aaataaagct
     7081 ggttttgtcg gaatttttca gaaatgacat ttactatctg gtgtaggaca aattgccgat
     7141 cgacaggacg gtttttcaga attttagcag taatgtcaag aaagtcattg ttaaatttag
     7201 aatcgaagaa agtcttaatg atcccaaagt tgaatttgag attatccgag acctttttct
     7261 ggtataaacc tgtaatattg tggaaatcgg catggttttc gacagtcttt tttgccttaa
     7321 tgatcttctg aaaacttgat ggtaaaattt cttctacttc ttgcaatatt ttaaattcgc
     7381 tattatttac agtataaaat aaaaatgtaa aggtgacttg atctttttga tttgctaatg
     7441 tttcgaagat atcttcttcc atttcgagtc ttctctgcct atctttttca tgggattgtt
     7501 ttagagagat atcagtgtag gctttatgaa tatcaacaag tatagggaaa aaatcatccc
     7561 gatcgatcgt tggtgccggc agtaataagt aattattgcc ataaaaataa cggtcaaaat
     7621 gatgttgaat ccattctttc ccgatgaata agtcagtagc acatgttggg cagactgggt
     7681 aattcttcca gctatcttcc cgccgaaatc cgccagcgat atatgctaat tcgtttgacg
     7741 aataaaagtt gtaagtactg cagaagccga aggtaacagg attctttaga ccacagatgt
     7801 agcaagcatt attttttccc tttgaaatga taccgccgta tttacaataa tattcctgtg
     7861 actggtcttc cagaaaatga tcaataattg gtttaaaaaa actactttct ccaggaagct
     7921 taccattgat gcgaatcgta caaatatttg acattgttgg atcaattttt gaaagtatta
     7981 tttcgattat ctgatcgaat tctttttgga ataaattaga gaggggattt aatgcggtgt
     8041 taactaaaga acaattttcg agagtattag ccattttttt aagagatttg cggatattgt
     8101 ttttttgttc ttcttgctcc ttgtttacag caatttgata agacgggaat tcagatttca
     8161 cgttggcagg agcgggttta agaagaatca atttattttt cgattgatca aattgcatga
     8221 catctacccc cagaaatttg caattttgtt catcgagaag aaaatcgaga atgataacat
     8281 ttcctgcctt aagcgtagta ttaagacgta agatgttttg attgccagta ttttttgcta
     8341 cgcattcgcc aatgattttg accttattta gcattttgcc tcctatgttt catgaatgaa
     8401 ttcgtgggat gatatatatt ttcgattggg attgattctc ccatgactga agtcatgtgg
     8461 tgccggatgg tgtcgggtcg tgttgtgatt cccatgattg gaaatatggg tcatcgaaat
     8521 tgtccagaat agatttacat atttgacctg agccacgaat tctcccaatt ttgttcgttc
     8581 attgaggagt ttaatgtagc ccacgaattc attcgtggac tggagaacga cagtgagtac
     8641 gaaaacgatc atatcgtcac tctcatttga ttaactccgg gtgataaatg gccatgaaac
     8701 ggtcatattc ctcctgaaaa gtcgtttttt gatgatgccg ttcctgattg ccgatatact
     8761 tctcgacatt cggcacactc gattcactga ccgataaggc gccgtaaccg acctgccagg
     8821 tgaatttcga gcgagttagg ttttcatgat taatccagtg cgagaaaatc cccttgagat
     8881 ttttgatgac gccggctata tcggcgtctg ccgacagcaa gaaaagacaa tgcatatgat
     8941 ctttgaccga attcacaacc cggactttac agttctgttc ggttagaata ttcttaattt
     9001 gttgattaag ggctgggcgc aaatccggag ggattatcac ggcctcattt tttgtccgga
     9061 agacagcgtg aatccagatt tttacgagag catgcgacat ggatgagtca tctccttatc
     9121 tcgtatccaa gaatgaattc gtgggttaac tcatagattt tggatacggg ctttattacc
     9181 cacaactgaa tttcccgatg gatcaggact aactttgaac cgaattcaaa gattggttcg
     9241 aatatcatct tgataccctt tttaccgtgc caaatccccg agcgacttgc ttaccgatac
     9301 cgagatagtc aggcaagagg aagtttgtcg taaattctcc ggtgaaacag accatgcgga
     9361 tatttttaaa gttcatcgga cgcggtttga aatatccctc gactttaaca ctttcgatat
     9421 tagggatatt gtaatgaaaa cctttagaaa cggtttttaa attctcacgc aataagtgcc
     9481 gcagaaactg ttgtttttca attttatcaa gagttttata ggtctcataa ttttcttctt
     9541 taagcgccat ccagggagag gtgaattgat aatcaaaaaa agaatcaatc tggccaaaat
     9601 tatcttctcg tatttggata ctcttttccc agattttggc tttttcaccc gcaatcacaa
     9661 gttcatatac atcctgaaat acttccttta gaatgtcaaa tccttcagat aaaccgatta
     9721 gagttgggat acgattgagc actttgaatt ggattgaggg gtagccgtac ttaaagcgac
     9781 cgtcagccag atgattatgg attagatcgc atttcgggta acggttagcg aaaaagccgc
     9841 gcagtttagg aatatcgaaa ttattcatcg ggttattggg aaactgaacc tggataattt
     9901 ttactatgat tgtcctactc attcgtcttg atacactttc ttcatttagt tgatttcata
     9961 atagccgtta accatctgga cctcaatttt accgatcaaa aatctgggaa agcatgaata
    10021 ttaaaagtct tcgggaatag gtcgttaaaa actttgccta aacttctaat attgccgact
    10081 tacaaaacca atttcaaatt ttagcagatc aaatatttct ctattcatac caaacttcca
    10141 aaccaaaata atttataaat taattttaga atatcaagct acttttcgta tcataaacaa
    10201 attctatatt tgtactgatt ctcgcatctt aaaaatggct aaataatttc cctatctcta
    10261 atttcggatt aagattcaat attatctgct ttataaggat tggttttgtt ataataaccc
    10321 aaaaaaggtg attattatag cgctgactga tctattccag acgctaaatt acaatctctg
    10381 agtgctttta attagcaccc attttctgat aaaaataatt taaatgtaat cggttttaga
    10441 atactgaaaa ttagattcag tatgagattg ttagtaaatt tcagaaaatg gaattaatag
    10501 tttttaacta tttattttga caggcggcaa aatctatcac agttaacaga ctgacagata
    10561 gtactcagct acatctcttc catatcgggg tattcgttct cttcgtcggt tctcttttgg
    10621 cgctctttga tgccctcgtt gattttataa gaaataccaa agaaaatcac ctgactttcc
    10681 gggtgccgca gtgatgtagt tttgaaatta tcaccgtaaa tcgtttggcc atgtttctga
    10741 gtcttgaaaa tgtcgctaac tcttacgaaa gccgacattt tcccatcgag gaaattgtgt
    10801 ttggcgccaa gatttacgaa ataattttcc tgcatacgtc cctgaccggg cccatgaaaa
    10861 ccacgtccgc ctcctgaaac gccaaccgtc ggagaaaaat agaaaagcat aatttgcatt
    10921 tcggtatttt cgccaagacg gaagaaggaa ttgacccggg cattccagct atgactctca
    10981 ttggcatcgg cgatggaaat cccggttcca taaagacggt tgttaaaata gctaatattg
    11041 gtattgatcc gccaccaggg tgttaaatta tgactgatga cgctctcgat accataagag
    11101 acaccgttat tgagattttg gtaagtagta aatgtgatac cgccttcttg taattccgtg
    11161 aggcgagtta tcattccatt tacctgacga taaaatagcg aagtgttgat cgaagttttt
    11221 cgattttcgt aagaatgctc aatctcggcg acgtcaatgt attccggttt gagtttgggg
    11281 ttaccggaag agagattata aggatcggaa taatctacaa aaggagaaac ctgccaggta
    11341 tgaggtcggt gaacccgacg gctgtaactg acctgcaaac cctgattttc cgtcagagcg
    11401 tagcgaaaat gaatagttgg aaacatactg aagtagtttt tctggaaaga cgagtcaatc
    11461 gtcactaagt tattattggt attaaactgc tcgattctta gacctaactg agattgtaaa
    11521 cggcctaatt cccgcgaata aatcccataa acggcgtgaa tatttgcatc ataaatataa
    11581 tggttggaag ccagactatc cggaaaccaa tctgtcatcg tataattata attaaagcgt
    11641 cggtaatccg aatcgttatg ccgaaaagtg ccacggtagc ctgcttcgat ccgtctatgt
    11701 tcgcctatgg gatgcacata atcagtgcgg gcggttataa cccgattagc atcgtcagaa
    11761 aaaatgtttt ccaaatctgg tagaacagtt ataagactat ccgttaaaac cgcttgctgg
    11821 gtaatagatt ggtcagaatt ccgtgagaat gttgaataaa ataagtcaat tgtccattcc
    11881 ctctgaggtt gaggaaaagt ttttttataa aataaaccgt attcctgacc ttgattatcc
    11941 gtgtcaccat aagagaggcg agtgaactga ttgtatccca cagcattata atcagtgttg
    12001 agatactcag tgttattttg attagagaaa gagcgcagat tgtagagcat tgatagtgaa
    12061 atagtattat aatcattaat gaaataatca ccacccagct tgatgttgtg aaaacggtta
    12121 cgattttcgg aattctgata ttggtttaga taaaaagtag aatcattgcg gattgactca
    12181 cggatagttt cgccagttcc aagcctatga cccatccgca tgtcataatt agcaaataaa
    12241 ttgaatttgt tatagcggta attaaggtta atggaaccgt tatatttgcc gccagttccg
    12301 gcatttacca gtaacatacc attatagccg ggcgagcgtt ttttcttgag aacaatatta
    12361 ataataccgg acataccctc cggatcatag cgtgctgaag gattggtgat aacctctact
    12421 cgttcaacca tactggctgg aatttcgtcg aggctgataa tctgtgacgg gcggctatca
    12481 accagcaagg taacgctgga tgaaccccgt agactgacat ttccatcaat atcaacggtg
    12541 accgacggaa tagtctgcat aatatcggtg gctgtgcctc cgatggttcc aacgctatta
    12601 tcaatagtga tgaccttttt atcgagatta tacgcgaggg cttttttctc tccagtgaca
    12661 gtaacggacg ataaatcaat tgccaccggc tcgagataga ccgttcccaa aaccacctcc
    12721 ggtttcggcg gtgtaatcgc aatattatta atgaccgccg ttttgtatcc gataaaacta
    12781 atcttggcaa agtaacgacc gaacggaatt ttatcgagga taaatattcc atcctcgttg
    12841 gtaatcgtac cggttataat gctggaatcc tggagaccgt gtaaagtgat tgccgcatat
    12901 tcaatcggct tatcagtatt tttaattaaa actgtcccgg aaagtctgcc ggatgtcggt
    12961 cgaggagcac tggctggtgg ttgagccatt atattgccac tactaattaa aattaccagt
    13021 actgaaagag taatagatag taccttaaca tttcgcaaaa tcataaatcg taaaatcctt
    13081 tggttaaaat tgttcggcta tttgattccg aaaaagtaaa aaggttaaac ttactttaga
    13141 attattagcc ggaatagaag cttgcggtac aactgggtag catttttaga aaagttaact
    13201 ggattcaaga ggtttttaac aagaatacgt caggcttacc attttttccg tttccagaag
    13261 aaaaagaaag ctacaaccga aaccaaagcc attcctataa ttatccaaaa agaagaagga
    13321 tgatttcttg gtaagggtag atcaacgttc atggaaaaaa tgctgacgat cagcgtcggc
    13381 accataatac cgatcgtaat aatagtcaag gttttcatca agacgttgag attgttgctg
    13441 acgattgaag cgcgggcatc cattaagctg gctaaaacat tcgattggat ttcggcttgt
    13501 ttaacacatt gagtattttc gattataata tcatcaagca attcgcattc ttcctgactg
    13561 aatccaattt tgacggtatt gatttttagt ttttcaagga gaacgccatt ggaattgatg
    13621 gcattaagat aatagactaa actctttgcc agactaaaca agtctattag gtatttattt
    13681 tctaatgaag tgtttatttt ctcttggaat tcatctgtaa ccatattaat ggcttttaaa
    13741 tgctccagaa aatggtaaat tgctcgtaaa atcagcttga gtaaaatgtc ctgattcgag
    13801 ttgacccgat taaaaatttt cccttcgaat atcggtatat cttccgatgc cacgatgata
    13861 atttgatctt taaaaagaaa tacgccggtc gaagtaactt tgaaaagaat attttcctta
    13921 gcggtgatac tgcgcggtcg cttgaaaata aaagcgatgt ggttgggttc gtactccaga
    13981 cgcgagagct catcggggtc gagagcggag tttagagtat gctcatctat cataaattga
    14041 tcaaccaaca ttcttttttc cgcttcatcg ggattaataa aaaccagaat ggaagcatcc
    14101 tcgcccccat tcagtacaat tcgcttgtct aagattttgt aacgattgag catttttacc
    14161 tcttgtctga aatctgctcg ttgattctta tgaaatattc tttttagaaa gcacggcgaa
    14221 cttaagcaat tattggctaa caaagaaaaa ttttttatta aaattcaatc tctgcgttcg
    14281 agggcaaatt gacgagcaaa attagctgtc gctgtactct ctcccgaacc gtagccgcgc
    14341 tttatttatt ctccggtaat ttaaaatgaa tatatcctac caagcgccat accacccaga
    14401 gaataaccag ataaaccagc ataagcgcca gagttaacca atatcccccg agggagttat
    14461 gccagaaatt cataggagca ttgagtaaaa tcaatagcgg aaagcccaga aatagagcga
    14521 gcccgccacc gagagccaag tcttcggcgg acggcgtttg gaattccgga tcggtgatac
    14581 gaatcagcac taaaccggaa tttatcgttc cggtcatttc accaaaaatc ccaaccatcc
    14641 gttcaaaatg aaagtcatca aaagctcttc gggaagtata gcgaattaca aaataggtgg
    14701 caaatccagc tagaattgac attacagcaa tcggtaacca atatttaccg acaattacaa
    14761 tattgatggc tacaatcgaa ccggtcacca agaaatcaac cgccacaccg gaaatccgat
    14821 tcatcaagcc attatcaatc acgtagcttt ttttagttat atcaaggcat ttacgaacca
    14881 gcaaagcaat cagtagagca atcacgaagt gaaaagacca gattgtgtca acgaaatcct
    14941 ttaaaccata cgtggtcatt aatttagtga cgaaataaat taacccgtag gtcattgcat
    15001 acacaatact gattagacca agctggaaag ccatcggttc aatggcttcc ggcgataatg
    15061 tcaaccaacc ggcaacttcc ggtttatttt ctttgacgac accggttcga atatcattat
    15121 tgatagattc gcttcccttg atcaaagtcg tttcgcggtt cttgataccc cgattgacaa
    15181 ttgccacccc ggcaaaataa gcaattaaaa aaccaatggc ggcaaaagtc aaaccgatca
    15241 tcccgccgcc ttcaaatccg aatttttccc agctgtgtcc catagtataa gctattcccg
    15301 gacccatacc gaatcccagc ggtactaata aacccattgc ggctggtaag tcgggagaaa
    15361 cggtatagat taatgccaga actaccaata gaccgatcgc tccctggaca atataacaag
    15421 tcaattccgt aaagcactta ctgaccggtc cttttccgaa tttagtcttg gattttccta
    15481 aaccaattac aatgaaagtt aacgccagga gatggtaaac atattgcccc agccggtttt
    15541 cgtcgaggta tatcaatccc aacacttgct gactgagcag aaggcctaaa aaaccgccga
    15601 tgatattatt tgggactaaa tatttctgaa aaaatggaat gtagcgtcgc gcgatcgttc
    15661 ccagcactaa gaaagccccc agagcagtaa aatctaaaat catatctttg aaattcagag
    15721 taaaatccca aggagtcatc atttcctctt ttcatcattt taataaaccg tctacttggc
    15781 ttttaatata gtaatctcga atattttata aaatcaattt aaaatatttt tgctctatag
    15841 ggactgattt tgataatttc tatttcgttt gacaaattac ttttactaga aaattataaa
    15901 atgatgatta ggccaataca tcctaaagaa agttcaaccc tcttgggctt taaaaagtta
    15961 ttacctataa aaagaatgaa gtactattct ccgtttctaa taattttcat ttttcttacc
    16021 gcccttttat ctccagccgc tgaagttgaa aaatatcaag gtaaaattga cccgggtaac
    16081 ggtcagatct atacctgtag gggagaaatc atccgtaatg atggcaaagt aactaaaact
    16141 tcttactttt atgacgagaa tggcaagatt cttctcaacg aaaaagtggt cttcgacgaa
    16201 aaaggataca aactcattag cctcgtatcc gaggaaccgc gctatggtcg cattcaggaa
    16261 attcagcgaa aagggagtca gtacattgcc cgctattgca aaaacagcgg cgaggaattc
    16321 cgcgagcata tcatcaatga tcagccgttg cttttacatg gctctaatct gccggtttat
    16381 ctgtcacaaa atctcgattt aatcggagaa aatggacata tttgtcgttt gattatagtc
    16441 ccaatgaaga tggaaatcga aatgatattt aaaaacaaag gaataaaaaa tattgacggg
    16501 catcaatgct atgagattca aatggatgcc gccaatttat ttttaaagcc actagtcaaa
    16561 actcattatt tttattacgg tacggaagag cctcactatt tatatcatta tatcggcacg
    16621 atttcactca ctgaccccag aggcaatgat atttggggta caatttcatt tacttatgaa
    16681 tgaaacaagc gctaagtaat tcgttacgat ttttaccggc taatcaattt caagaattca
    16741 gcccgcgcct gcggttcatc atgcagttca ccgagcataa cgctggtagt gatattggaa
    16801 ttttgttttt cgacgccgcg catttgcatg caaagatggc gcgcttcaat gatgacgccc
    16861 acaccttttg gttcgagtgc ttcattgagg gtatcagcga tctgctgagt caggcgttcc
    16921 tgaatctgga gccgttgcga aaaagccgta acaacgcgtg gaattttact aaagccaata
    16981 attttaccat tgggaatata agcgacgtgg gctttgccga agaatggcac cagatgatgt
    17041 tcgcagaggc tgaagaaatc caggtctttg acgatgatca tctggttata atcttcgtaa
    17101 acgagtgatt ctttgagcat ttcaagcacg tccactttat agccttgtga aacgtgttgc
    17161 cagaatttgg cgacgcgcgc gggcgttttc agcagttccg gccggttagg gttttcacca
    17221 atattgacga gtaaattgcg gatgatttct tcagtttcca tataacctca gtttaatgtt
    17281 tattcagatt ttaattgttg taatttttgc tctaattcaa atgatttttt ttgacttaga
    17341 atttcaccat tgatccaatt cacgtgtgct ccagccattg taggcggctt cgttccaata
    17401 gaaggaacgc gcctggtgat tctcaattct tagtaacaag cgataatgag tccaggttaa
    17461 ttctgggcgg atcgaattac ccaatcttat gacacttttg ctatctccat tagcaagact
    17521 atctgataat tctcgacgca ctgcgtcgag aattggaaat aggagataaa atttgcgcat
    17581 ataccacaag ttacttggat caaatccccg accgtatgcg accgttagtt ttctggaaag
    17641 gtcggcaatt agttgcttcc caaattccgc ccttctttta cctttttgtt cctcatcaac
    17701 gataatccgt ccgatattcc aatagacttc aaccatgata aaattgaccg atcggtaagc
    17761 tctggaacgg gcatcctcta aaatacttcg gatgttattg aaaatagaat cggagacttg
    17821 agataaatta ttcatttatt gtttctagcc gagggacaaa tatcattcaa aacgcatttg
    17881 ctacactgtg ggcgtcgggc gatgcaaacc tgccgaccgt gttcgattag cagatgcccg
    17941 agcttggtcc attgttcttg gggaaattgg cgcatcaagt cctgttcgat tttttccggg
    18001 ttgctgtttt tcgttagtcc gagcagattc gccagacgct tgacgtgggt gtcaacaacg
    18061 atagccggta ctccgaaagc atcgccgaga accacattag ctgtcttgcg cccgacgcct
    18121 tcgagttgca ggagttcctc cattgttccg gggacttcgc cgttgaattt ctcgacgaca
    18181 gatttcattg cggatttaat ggctttggct ttgttattgt aaaagccggt tgagcggatg
    18241 tcgtttttca gttcttcgag gtcagcatcg gcaaaggctt gcgccgtcgg atatttctgg
    18301 aaaagagccg gtgttaccat attgacgcgc ttatcggtgc attgcgccga tagaatggtg
    18361 gcaattagta gctcccgcgg attggtatga ttcaaagcgc agcgagcgtc cggataggcg
    18421 gcggagaggc gggagaagat ttctggtgcg atttcagatt tattagtgat aattttcctc
    18481 ttagatttag ttacattaat tttagatgct gaaaaatttc aacaattcct tcgtatcatc
    18541 cgaaatagtc agtagataaa gcttcatctg caataaactt taactcttct tgagttaatt
    18601 ttctatctaa ttcctgttcg gcaacagtct gaacgtccgc aatattgatt gaatagatta
    18661 ttttatcact tcgcattttg ctcacctcct tctaaaaatg gtctgataat atccaaacga
    18721 tttgtattga ttttaccatc aggtgagaat gggcactgag ccagtattct ctttaaagtt
    18781 ttatccttta ttgaaagatc attcaagtgg ggaaaatttt gaataatttg taattcagta
    18841 aacaaaaacc aatcgtgcaa aattgcttta cttttggtag caatttgtaa aataggctgg
    18901 tttattatat caaatttatt catgaaatca ggttttgcaa ttgaattgat tattgagaga
    18961 atcagttcca ttgttcctgt ttctccagaa agaaaattcc ataattcatc agcaagtaat
    19021 atctcctgtg gatggaaata ctttctaaaa tctatagaat atccgaaata tctttcttta
    19081 tttgattttg ttggttgttt acttgtcgga tcaaatggaa atcctaagta ataagacact
    19141 tttttgccta aatatttatt tctaagacca gtttttgcct gtaatatttt ttctttttct
    19201 actttaaaaa caccactgtt cggttttacc gtttttaact ctatagcaat tatttcatta
    19261 tcagtctcat agaaaacatc ggccgtaaaa tttggtatgt ggcgatctaa tatttcacct
    19321 tgatatttaa gaatattgtc ttctgaagaa acatcaggct gtctttgacc gtttttaagt
    19381 tcagtaataa tatctgaaat tactgtttct tgcctactac ttataattag gtcccggaat
    19441 tctctttttt caccattaga taaaatatgt gatactcgtt caaagaatga ttgaccaaga
    19501 gttgtactta gcccatgtaa ccaactacta agagttatca aaaaaggaac agattttacc
    19561 tttccctcta atttaacgga aaaagcattt aagaaagctt catgaaaagg cgcatttcta
    19621 ttgtccattt cattttctgg aaaagtatca aagcgggatt tgagggtttt tattacctca
    19681 aaagcaattt tctcaatttt agcatttgtc attgaacgtc cttcaaatga aagataattt
    19741 ctgaataggc acctttatct ttttccgaac gatttaaaac aggcctttta taacagttga
    19801 cgattttcat cccggatttt tcggcaatca taggatatag gttatattta tcattagcca
    19861 caagaaaaat actataattg ggttttaaat atctcttaca atttatcaat acatctgtaa
    19921 taccctgaac gtacgattct cgagcttcta ttcctttccc tttaaataaa ggaccaattt
    19981 ctaattcgtc atttctctca aagccaaata aatcgtaggc gtaggcatgt tgttcatgat
    20041 aattgataag accgacgtat ggtggacttg aaaaaatgcc gtctattttt tttctctgta
    20101 ctaaatccga caattcttta ttcttgcttt gtagtttttc aattatatct atgtttcttg
    20161 aatcgcccac aagacagtat tggaaggtat tggttcttaa tttgttaaaa gttgccaatc
    20221 tcaccaaagt atccttacta tatctttccc accaattaag tatggtaaaa agtggtttgc
    20281 atattttacc atgcttggca caataataag gtgttgctac cggttcaatt aaggtcacta
    20341 aatcagcgtg ggtcgtagcg cggcaggagc gaattgttct actgaatata acactgatta
    20401 tcttttttat ttctgaatcc tggattaatt taatttgttc aaaaacaaaa tccatttctt
    20461 ctcgaatcgg ctgaaggtac cacttttcaa tgaaattatt ttcagtatcc tgttttaatt
    20521 tgacgccata tttttttact aaatcccagt agattggtag aaattcatcc gatttagttt
    20581 ttccgtaatt atcctcattg atttctttac gtctgacctt ttgtttatat tcaggtgaag
    20641 ggaaatatct attattaaat tcattgagtt tttcagtcag ttcaacatca aactgcagaa
    20701 tatttctctt cgagttaaaa acccggagtg atcgtgttat tttcgtaatt tctgactgta
    20761 gagagaacaa atcatacttt ttaactttta cattactgat caaggcatta aaagcagaaa
    20821 catcaactcc tatagcgtgc attcctaatt cattggcttg aactaaagtg gttccacttc
    20881 cgcagaatgg atcaaggatt atgtcgcctt tgttaaaaaa aacttctgtc ttaaactcgt
    20941 caatatgatc atcaagaaaa tattctacta gttggggaat aaatttccct ttgtatgggt
    21001 gaagccgatg aacgtgtttg gtggtatcgg cctcttttaa gaaatcaaaa ctcaaatgcc
    21061 aatttaaatc ctctcctaac tgatttttcc aattaatttc acgcttaccc cagaacgagt
    21121 cgtaatagtt tttcagctcg gaaatgtcaa cgtaaatcga gccattatta ctggttttat
    21181 ttatttttcc atagttaatt aaataggtga tgttagattc ggtgactttt ttgtttaagt
    21241 aatcacttgc ccaacggctg gcatctttta gcgaaagaat attattttgg tttttaaata
    21301 atgtttccat ttcacccatt ataattcacc ttttccaccc ttgtaatttg cctttttcgg
    21361 agggggaaca agggcttttc agatctaaaa ctgtcatttt ccgatgtttt gtgactgaca
    21421 tactttacat aaatcatgtt tataaacttc ttcttctgat tcagtgtttt cgaaaagaga
    21481 tagatcactc ttaattacgg tttcaggaat aaaaatattc gggtggagct ttattttgga
    21541 atattctatt tccttaatgt tatcgggaat tacaaaatat tttgttaact ctacccaatc
    21601 tttatctcta attgtgattg gctctcctag gaaaaaactt aaccaaattc cattgatttt
    21661 agaacataat tcttcctgta caagtttttt ttgagtaatg gttatatagt tctcatctat
    21721 atcaaagcca atatactttc gaccaagtcg tttagccgca attgctgtcg tccctgtgcc
    21781 tacaaaagga tcgagcacaa tgtcaccttc atcggaggac attaatatta ttctttctaa
    21841 taaatgaacc ggtaactgac agggatgttc atctctatat ttattatgtt ttaggcgatg
    21901 aatatcggtc cacacatcag aaactaaagg accaaagggg tgtaaaccag cctttttgcc
    21961 gccataatct tttaagagat acccgcattt tctgcatcgt ttatgggggt agcgaatttc
    22021 gaaaaactta tttttcttta ggtcttttgc gtaatataaa ataccataat gagaaggttg
    22081 taaactttga cccattgggg cagttggtgc atcccaggca atccaatgtt tgaaataagc
    22141 aatttcattt aaaaaacttg catagtatgt taaccacttg ggtatattgt gcacaaatat
    22201 agaacccgca ggttttgtaa ttctaaccat ttcaaatatc caccgtttgc accaatccaa
    22261 atatgcctga acctctttat tgtcccgata cccattatat ttcttaccta aattaaaagg
    22321 cgggtctgcg aaagttacat caacactgtt atcggggatg tttttgaata attcaataca
    22381 atcgccttga attatctgat tgtaatattt ctcaaacatt tttcatcact tcacataata
    22441 atttctcaga tctattaagt tcctcttgat gaaaggtaaa tacatcttca taagcgccca
    22501 ccatcctttt taaatccctt tttctaacat accaaccaat accgtcaaca aagcctaaaa
    22561 agcgagcatc tgggtaatgt tccttaatta aagagtcaat tgagatctct gttttcgatt
    22621 tatcaccttg acctgaagaa gttgtgacta aaaaagtact ttctattata atttttgggc
    22681 tttgtttatt tggaataata aaatccattg ttctcttttg ttcgggtgca tattcaataa
    22741 gttcgctgag atcacctttt tcaaaaggaa ttttacgttt ctttagaatt ctttcgataa
    22801 gaatttctgg gttatttttc cctttagcag ctctcgaacc tttttcttta tatcgtgcaa
    22861 gagtatcaat gattgcattg atatcaaaat ttagcttttc gagacttaat ttttgaaact
    22921 caaacggcgg taaggttctt ttgaaaaagt ctattgacga accttcataa aaaatattga
    22981 ccagacattt tctaaaatat tcattttccc gcaccaattg ctcaattttg gaggtattcc
    23041 attccgaaat ttcattatct ttggtcgttc ttttattatt aatccaataa ttcataaaag
    23101 taaaatcatt cagctcttta tcgtccacaa ttctgataat agtgatcaac cttaataaac
    23161 tttcatttga gaatcctgta atggcgagta atgccttaag gccgagttct tttttattcc
    23221 ttaaaaaatt ttcgaagact tcttttttac agccattatt ctggacctcg ttttttaaat
    23281 gtattataga ttccttgaca gaaaaaatat gactttcata ggctgattcg aattcttcat
    23341 caaaataata atatgtatta ttctcaatta ctgttctata cttctcgtcg attgttttca
    23401 tatttagttc ctatctggat ttttctggaa gaaaagtaaa tactcgtctt tcatggtatt
    23461 tctcattcct ttaattggct ttaggatgct tttaactaac tttaatccaa tctcttcaca
    23521 attttggatt atagtttttt ctaatcttat cccgccggtt tgatttgtat tcgaaccaat
    23581 tatcataacg aaatatttgt catttttcag caccctatat atttctgaac agactttttt
    23641 catatcacga aaataattat ttaatctttc gtctttattt ttgccaatta acccaatcat
    23701 cttaccttta attttctcga tattataccc aagatattct aattgggctt catcattctt
    23761 ggcataatca atcgcgaaag aataaggggg agatgtaatt acaccatcta ttgagttatc
    23821 cacgattttt aaatctaaag catccgaatc cgataataca ttcagtttac ccagttgtac
    23881 ttttttgaaa taggaattat taataaaatt tcgtacggta ttttcatatc tagttaaaac
    23941 tttttcgaac aattgcctgt gatccgattt aatgacacgt tttgaatacc ccattgaatc
    24001 caagtaagcc aataaagcta aattatatat ggcaagtttt tcattatcag taatgttctt
    24061 caattttttt aaaatatttc cttgtgaaaa gaaattgaaa agcttatcgc gattttcaat
    24121 aaggagagaa agcgagctat tcgttatttg taatgcatca tatttagctt gtgtcataaa
    24181 ttggcaaaaa gggcttatat caacggcata cgaattaata cccatcaatg cggcttcgat
    24241 attggctgta ccacttccgc acattggctc taaaattgtc tggccttttt ctactccgat
    24301 tatgttaaga agagccttga ttaactgagg atgaaatttt ccgcgatagg gaaatagtcc
    24361 atgggtagca taaccagtag agaaagtacc gttttcaaaa taatttttgg tgacattgga
    24421 tcgattttcg attagcccct cacaggtttg gttatacatt aaataatgcg ttgtgaattt
    24481 gccatcaatt gattgaatat aggccgaacg ttcaataatt tggtcttttg gaaggctttt
    24541 gtattcgagg taagccaact cgagttcaaa taattcggta actgatggta aaagagttac
    24601 gtttttatcg aatagagttt ctatacaggc tggtaattca tagaaggaat aatttcccaa
    24661 aactttcata acctttcatt aacaattgat aaagccattt cacccacttt caattcacct
    24721 ttttcaccct tgtaatttgc cttatttgtg agcagtaatc aagcgctttt tatacttatt
    24781 aattgtggca attcattgaa ctgtttcact caactaaaaa tccttgatac gtgccgcggc
    24841 gcgccaattc atcccggatc ccagttaata tctcattttt ggtgagattc cactgcgggg
    24901 caatcagcgt gccgggtttg gcatcgccgc acaaacgctg gatgacgatt tccggatgta
    24961 agtgctccag gaaatcaacg acaagcgtta tccattcttt ttcggtaaat agtgagaagg
    25021 gattttcggc gtagaattca gccagcggcg tgtttttgac aacgtgcagg tggtgaatct
    25081 tgagtgcatc aattccaaga ctattcaaga caccgggagt agctaaaatg tcagtgcgcg
    25141 tttcagtcgg aaatccgaga attaggtgtc cagcaacgtg gagaccgtgg gctttcgccg
    25201 cctgaatcgc ccagcgagtt tgagcaaaat cgtgtccgcg ctgcgcccaa tccagcgttt
    25261 tatcatagac ggattcaata ccgaactcaa tggaaatgta agtttcgtga ttcaggtgtt
    25321 cgagtagttg taatatttcc tccgaaacgc aatccgggcg tgtgccaatg caaagcccga
    25381 caatttcggg aaaagctaaa acctcgtggt aaagtctttc cagctttgcg accggcgcat
    25441 aagtattggt aaacggttga aaataagcca gaaatttatc ggcctggtga aaacgccgag
    25501 ccgacgctat gcctttttgg atttgatcac taagcgaagg caattcttca gtacgctttg
    25561 gggcgaagct ttcgttattg caatatagac agccggctgt acctttggtg ccgtcacggg
    25621 ttgggcacgt aaaaccagca tcgagcgtaa tccgataaac ccgtgtctga aagcgctcgc
    25681 gtaggtagcg accataacga taatagcgtt catccagcgg aaagggaatc actttttaat
    25741 taaaattcca gatttaagaa tctttaattt caaatcgaaa ttcaccggtt attcagctaa
    25801 ttgactggcg accagcaccg ccagatcgag gcattttacg ctcgaatttt gttcctttaa
    25861 tccgtcataa agcatagttg tgcagaacgg acaggcggtg gcaataatat ccggattcaa
    25921 tttggtgagt tcctttgccc gcagggtatt gacgcgctga gctggggctt cttccagcca
    25981 catccggcca ccgccggcgc cgcaacatag agcattacta ccatggcgag tcgcttcttg
    26041 gaaatcagct tgtaatgccg cgattatctg acgaggcgca tcataaattc cattgtgccg
    26101 tccgaggtag caggcgtcgt gataggtcaa tttgcctttt atcggctgat tgattttcaa
    26161 acgtccctcc gctaatagac tggcaatcat ttcgacatga ctgataacct cgaatttgcc
    26221 acctagctgg ggatattcat ttttcagagt attgtagcaa tgggcgcatt gggtgataat
    26281 tttttggacg ccgagattgt ggaaagtctc gatgttttga ttggctaaag tctggaaaag
    26341 gtattcgtta ccaagccggc gagcggcgtc accggtacaa gtttcggcat tgcccagaat
    26401 ggcgtaattg acccgagcgg cgttcaacaa ttttatcatc gcgcgggaag ttttttgagc
    26461 gtgttcatcg aaagctccgg cgcatccaac ccaaaagaga tattcaaagt cggctttttc
    26521 ctctgcccgc ggaattgcca gaccttccgt ccacttttcg cgatcagagg cgccccaggg
    26581 attaccgtag ctttccaaat tacggaatgc cagttctgcc gcttccggaa aacggctttc
    26641 ttccagtact aggtgtcggc gcatttcgac aatccgatca atgaactcga tgctcagtgg
    26701 acagacttct tcacaagccc ggcaggtggt acaagcccaa agtgtctctt cgccgatggt
    26761 gtcgcccacc aatttttcag cgcgtggcga ttgagatttt tgtcttttat ctttaataat
    26821 atcagtcgtg tacttgataa gatgatgccg taaagcaatg ttgaactcct tcgggctcag
    26881 tggcttgccg gattcgaacg ccgggcaaac cgtctgacaa cgaccgcatt ccgtacaggt
    26941 aaaaatatcc agcaaatctt tccagccgag atgttcaatg cgggacgccc cgaagcgctc
    27001 gccattttcc acatccaaaa agcgcacata tccactgtca agccgtttga aaaaaaccgc
    27061 tggcaaggac gttataatat gaaagtgctt ggaaagtggc agaaatggga aaaaggataa
    27121 cagagttata agatgcaacc accaattaag atgaaaaaat tttacggcga actccggagc
    27181 agtattacaa aaaattcggg caaaggctac cccgacgacc gtccgatcgg catccgcttc
    27241 aaacgcgacg gtcaacccgg tcattaaaag gtctgtaacc atcaaaatcg caatcattag
    27301 caaaattaga ttcgctgaag cggataatgt caggcgtttt gggcggatga tcaggcgctg
    27361 aataagggca taaatcacgc tgagcagaac taaaacttcg aaaacatctt tgagccagag
    27421 ataaatctgt ccgagagtct gtccagtatg aaaacccggc aggacgaaat tgggattgaa
    27481 tccctgccca atcatttgaa tcgtattgat tgaaaccacc agaaagcccc aaaaaatgaa
    27541 agtgtgcata tagccagccg accagaaacg ccgatcacgg attttcgttt gagcaattcc
    27601 ttgtgtgaaa aaagcgccga ttcgtcgtcc gatatggtca aagcgatttt ggggctggcc
    27661 agccgcaatt acggcaattc tccgatacat tagccaggcg aaccagccta gtgcgactac
    27721 cagcagaact atgaaaagcg ccgagccgag tggggtattt gttggtgcca tcatttacca
    27781 gtttctcctt ttttaaggtg aaattttaaa catttccagc ttgattccaa aatcagagag
    27841 ggtctcgttt aacccaccgc aatttttgtc tgatctaggg acatggtatc cacccttagt
    27901 tattctttcg actttcagaa ccaaacggaa gagacttatt ctgatttctc actcacatta
    27961 ggttagaaaa aaaagcccga cttttaatcg ggctttttta ttacagtaag gaatttgcag
    28021 gttactttat caacaatatt tttatcgttt tggagtaatt accagattgc atcctaacca
    28081 gatacgttcc gcttgaaagt agcgagccat caaaaacaac cgaatacatt cccgcctgct
    28141 ttgaaccttt tactagtgtt gctacttctt tgccgagaag attataaact tttaatgtca
    28201 catgcccggc tttcggtaat tcataaggga tagttgtagt cggattaaag tctgttattc
    28261 actgcgttat aagtatcatt cggttcagtg aatagcgtag ttacttcgta gtacggagat
    28321 ggcgccgaat tgacatcgcc atattggtta aatattttac ccttaatttt gtacttcgta
    28381 ccgtatttct cctgccatac tacaccgata taattatcga caacatagcc ttgattatga
    28441 gtcgtaaagg caatcgaagg acacttgccg ccggcagtgc catctaatgg ctgtccatta
    28501 ttgccaaatg tccaggtact gccaccatca ccggaatgct cgagccacac gtgaccggcg
    28561 ctggtgtaaa cctggtgaag ccaacgggga tttccaccag ctaaggtctg aatcagtttc
    28621 cgctggctgg tattatccca agcggcaatg gtattggatt tgtgaagggc ttttagtctt
    28681 gcattaacgg taatatttcc atttatctct cggtaattcc cgccaacgcc gtcgctccat
    28741 ccggcaacac cgaaaccaga tggcggaata gctttgatag attgtcctat gccattaaac
    28801 gatgctccaa catttattcc gtttacttca tacgtgcttc cattaccagt aatatcaacg
    28861 taggttgcgg gattgagtgt tatgatccct ccactttgaa tggcgattgt gccacctgtc
    28921 gaagtaagag aatatccatt gaaatttacc ataacaccgc taactattgt caacacaacg
    28981 ccggatggaa cattaacatt accttttaac actttgtttt catcccatgt tgtattgctc
    29041 gaaatagtac cgaagggtcg ctggtcgatt attgttccgc ctcctgagta tattgtgtaa
    29101 ccgttttgat ttaatacaag atctgcacaa agggttaagg tcacaccgct tgggacgtat
    29161 acattacctt gcagtgcaga tggagcagac caatgttgat tgcgcggtaa gactcctgat
    29221 agtgtataag acgaattata aatacttaac aaccctgaga aacggtctcc caattgcgga
    29281 tgcattgggt aattagggtc cacggagttt gataatgcag ttgttaacgg tgccgcataa
    29341 ttatgcaatt gttctgcggt cgttatgctt gatttggcaa gaccatctaa taaataatac
    29401 gaaaaaaaac catgctccaa tcccagagaa ccgtcggaat aatcttcctc ggctaattcg
    29461 tccgcctgac atgcagaaga tattaccccc tttgtcataa cccttggaaa tattccggaa
    29521 caacacgcat ctaaaaatgc agtactttgg ttaaaggttg agccaagatc tgcttgtaat
    29581 tcacttggcg tgatcattgc ccaatcaggg gcataaagac catcaccgtc agaccctccg
    29641 tataacgtat ctaattcgcg tgaatttccg tgccccgaga atgaaaataa attggtgttg
    29701 ccagcacttc ttggcatgtt ttgtattgca gtgtgtatta cagactcgct cgcatcattg
    29761 tctagcaaac gagttatttt accactactc cattgtttat aggtaattaa atactccttc
    29821 atttctattg catccttaac acaccaatga agattattct ctggatcacc atagttatta
    29881 attcctacac acacagcatt ccaattctga gcaaatgcgt ttcctgtaat taatattatt
    29941 agaaccgtaa taatcgttag gtttttcatt gctcattccc tctttctttt gcttgccctg
    30001 gtcttggaat cgctttgaac catacagaaa tctctttgtt gttttgtatc atgcgtgcat
    30061 aacttttccc atcctccgta atattcaata cctgatcatc gcctacgatt gccgacaaac
    30121 ctctcgattt tccagatgca ccaatcctaa ctacgttgtt cttgcttcca gtatcggtca
    30181 gattattaat tgcaataaat ccaccgccaa gtagtttctc cattattttt agatcggctg
    30241 ctcttattct agtaccgtgc tttttccgaa acgattgtat gtccgagcac acaatcattc
    30301 tttctcccgg ctttagttct attgacttct gcccttcgta ttctctgggc aaaacagagg
    30361 gccttcttat gccggaaatt ctcatactgt taagagataa tgtctcgtcc gacggattga
    30421 atatctctat ccatacttcc ctatttgttg aatcaaggta gacttcggta acaactaatt
    30481 gtttagttgc atcctgtgcc tgaagcagag aaactgttaa aaagaaaaca atcaacaaaa
    30541 aagatatttt gaaatgtcga tttctcatga ttttattctc cattttatat aacaaaattt
    30601 gatgttcatt ttttaattgt atttattgca gtaaataaaa aagtaaagac tctcccattt
    30661 aatatttatg atcgtcaccg attgtgtttt ttgtcgttac tctacgggcg gcgcgctgca
    30721 acgctgcccg tagttatttc aatgagtggt acataatgcc cccctatttt ttttaataga
    30781 atactgatac cccccgtgct tgaagagctg gtatatattc attgaccgat ttttcattta
    30841 aaggatttcc gtctaaataa aggtacacgc ctttcccaag attagcatta ttcactagtg
    30901 gtaaaatatc ttctatctgg ttcctgctta atccgattaa atggatattc ggtaagtact
    30961 gcagttcgct gatatccttt atctcattat ctgccgcatc gagataatca aggctctcca
    31021 aattctttat tccactgatg tcgttaatct tgtttaatcg tatatataac cttatcaatt
    31081 ttttcatttt cgatacggga gaaatttccg taagattatt atagcctaca tctaataaaa
    31141 taagttctgt tagattttct aatggagtaa tatctgtaat gttaccacct gacaaatcta
    31201 attgttttaa attcaacaaa tttctaagag gttcaatact ttcaaatgta attccttccc
    31261 caacaccact taaatggagt atttctaagt ttgataaact acttaaggga tttaaattag
    31321 tgataggtgt aaaccacagc cacaattctt ttagatttgt taaatttccc aaagcactta
    31381 tatctttaat aggattggta tcaagaatca gttttttaag gttcaccaaa tttgtaagcg
    31441 gtgaaatatc ttctatggtg cgattctgac taaggtctag gtattccagc ttggtaaggt
    31501 cagctaaagg tgccaaatcc gtgatactct gcaacccaag agctaagtat attaagttcg
    31561 tgcaattctc tattccttga atactctttg ttttataatt cttcataact acatcactag
    31621 ccggcaatga atccagcatt tgtaatttgc cagcgtttag ctctccctct ttatagctaa
    31681 gttcatatcg tatgcgggct tcaagataaa tatcctcaat cacacttggt tggcgaggat
    31741 tgactgttat tacaacatca tccgtaaaga catttccgct attgcattca atgttcagtg
    31801 tatatttata tcgtccctct ttttcaaagc tcacaatcca tttatcattt aatgacgatt
    31861 gtgcaaataa atatatttcc tgaggatttc cggcgtcttg cgtccattct atcaaattaa
    31921 tggcttcacc ttccggaagc atactgtttg atatatcaat taccacgtaa cttcctatat
    31981 atgtcgtttg atcttcgcca gcatccgcac cttctttttc tgattcatta ttcacaattt
    32041 cattacagga tatgaaaaac aaaataaaca atggcataat cttgatgtaa ttcattgttg
    32101 cctcagtata aataaaaatt ttcttcattt taattctcca taatgttaaa aaactattcc
    32161 aatatttgac tgaagataat agtttgtaaa atcaatatta attatatcat catggtattg
    32221 agtaagtccc tttaatagtt taacctcaaa agaatatgta ttacgacgaa tattgaattt
    32281 tttaattatc ccaatatcta atatgaaatc aaaatcagga aaactgcctt tcatattctt
    32341 tttaaatcca atattaccaa tttccctatc ataaattgtt aggtttgatt ttataagata
    32401 accaatatct atccctaata aaattcctag cttatcaaaa atatttaaac ccaagttatt
    32461 cgacagtctt aagaaatcgt ttttataatt agcataacaa ggacttacag cggcagctgg
    32521 tgagtactta accatagaat ttgtataaga aaaataaaaa tcatttagga gaaaaaaatg
    32581 ggtattaaag gttatagtat ttgacaaacc catatcaaat cccagaggat ataaatactt
    32641 aatcggttca agatgggtaa agctttgttt tgtaaaattc atcccaactt taaagccagg
    32701 attaaaatat tttatatctg ccccaaaaga aaaaatttct aagatcatta ttaatagtaa
    32761 gactctaatt ggtttcatgc taaatctcct acagttaatt ctcatatctt tgaatacgaa
    32821 aaataaattt agacctccct gtctagcaaa caacttgata tttgagaaaa aaagagaaat
    32881 ggtaagagac agtaagccct cataattttc agacttataa accttaaaaa gagtcctacc
    32941 actgccccta aagttgccat tatttgattt gttccgattg ttcggaatat tgtttctttg
    33001 gaaatctgaa tcttgagaca aatccattcc tttcctacaa tccttccctt ctgagttaga
    33061 aataaaaaat cctacggttt tccgctcttc tgggttcatc gaagactctt cttgcttctg
    33121 gtataatcta tatgatgggg gggtaaaatc aagagttttt taggaaagtt ttttgtaaga
    33181 ttatttttcc tgccagacga taccgatata attatcgaca acataacctt gattatgagt
    33241 cgtaaaggca atcgaaggac acttgccacc ggcggtacca tctaatggct gtccattatt
    33301 tccaagcgtc caggtactgc cgccatcgct ggaatgctca agccacacat gaccagcact
    33361 ggtgtaaatc tgatgaagcc aacggggatt tccaccggct gaagtctgga ttaatttccg
    33421 ctgactggtg ttatcccaag cggcaatggt attggatttg tgaagggctt ttagttttgc
    33481 attaacagta acatttccat taatctctct gtaattacca ccaacaccat cactccaacc
    33541 ggcaacacca taacctgatg gcggaatagc tttgattgat tgtccaatgc cattaaacga
    33601 tgctccaaca ttgattccat ttacttcata cgtgcttcca gtaccggaaa tatcaatata
    33661 gctagctgga ttgagtgtta tgattccacc actttgaatg gaaattgtac cgcctgttga
    33721 agtcaatgaa tatccattgt aatttaccgt cgcgccagag ttgattgtaa gggtaactcc
    33781 atttggcaca gtgacatgac cagtaagtgc tacaattcca aaccaagttt gatcatatga
    33841 cagtgcgcct gaagttgtaa atggtttagg agttggattt tttaaataag tttttacccc
    33901 tgaaccatta tatcttcctt tggctgggat actaaaagat aaatccccac ctgttcttgt
    33961 tttggggcca attgcaggat atgaatatcc gtctaaaaat tctggctttg aagaataata
    34021 atatgaaaca tcatccaatc tataagatga ataagcacca aaatcatagg caacattatg
    34081 aggcacggga gttgtataat tattcaaaaa accatataag tcagttgctg ggacagcatc
    34141 tcctgtccaa ttaaaacgta aaggatataa taaatcaccc tgtttttcca tatttcccaa
    34201 ggtcgcccat tcatgcattg ttttaataag tgcaataccc cccgtagcca tgtttcgtat
    34261 aaatgtatta taaggcccgt tatcgccgtg ataatcatct gacgcaattc tttcaacaat
    34321 attgtgttca aaaagatgcc catatggata tttggcatgc agatctagat cgcgatcgtc
    34381 atacccaatt ccaagccacc aagtagaata ctgctcccgg gaatagttaa atgtccacac
    34441 attacagttt gagcctccac ccgcaatcat tgcatgacga aggtacctga aaatattgtt
    34501 ttcgatgagg caattagtcg agctaccaga cgttacaaca ccgtaacccc aaccgccgcc
    34561 tccataatac atcgcttcgt gaaaatagca gccggatact tcacaatgag acgatctata
    34621 tatgcctcaa tgattacatg ccgccttata ggattcgact cctttaatcc agcaattgat
    34681 tgcatagaaa agttgtatat tgtagggatt attactgggt ttattattag gagatcgtat
    34741 tatctttaaa ttttcaattc caatattttg aattggggtt actttacaaa cctttagtga
    34801 ataatccgta ttcactgcat cttcatagtc catattggct tcatctttaa tttctttcca
    34861 gtcaccagta gcgtcggagc cttgagcact gattcttgtg atttgaccga caatttctct
    34921 ttcaaagtca cgttctgtat gataatcaaa atttctttgg atgaaatgaa tccagtcacc
    34981 aactaaaata ccacttaata ctccaacagg agccgcatga attacggatt ctcctttatt
    35041 aaaatcctgg tccaaatcta tccagtcgcc cgttgttcct gtcaaagcaa aacaaggcga
    35101 atttttcata ttttgaaaaa caagcgtggt cttgtccgat ccagctccct gaaaaacaat
    35161 gttgcgatca ttttgtgtta atgttatggt gctggtaaaa ttaaaggtcc cctcgggaaa
    35221 atagataatc gctaaaccac cagtgcggtt gatatgatct cttgcggtgc caattgctgt
    35281 cgatacttgc gaactggcag cgcctgggct aattggtata actagtttag gctccacaga
    35341 actcatattc tgcaaaagac ccgctacgtt ccaggtctcc tggtagtaag cagggatatg
    35401 acctacaccg ttgtacaaag tctgtgactt aattccggct ataagtatta aaagaaagcc
    35461 aaaaacaatc ataatcaaat gatttgtttt catgttgcta tcctcctaat taatcaggtg
    35521 aaaattttta gttttagaat agaaaagcca tcgatatttc aaaggtatct attttatctc
    35581 ggacacttaa acttgtaaca ccttctgtga cagataatgc tcttgcataa ctggtaatta
    35641 ttgctaatcg tttaaaagat agtctggcac ctaagataaa atttgtggac ggtattgtat
    35701 aattttcatc gagaggaata taatcgaagt tatatgtttt tctttcatcg ggtccgagtg
    35761 gtatggtttt tttatctgaa atttttgtat ggttcttgat tgggattgaa aggctgtaac
    35821 cggtgtagat atttgaaaca aattgattat taataacaat ggaatagcca acgtttatag
    35881 gtaattcagc gtacaatata tttacattga tatctcccat ttctatatcg gatatattat
    35941 aataaggatc gctctgccaa gttcggtttt ctaaaatgta ctttttccgt ttaaaatcta
    36001 ttcccaagtc gaaaaagcca ttaaatgaac gtattgggta aaactctttt cccgctccaa
    36061 agcaaagtcc cggttcattt ctaccttctt ctttgcgaaa agaggaaaaa ttggcgccta
    36121 ttttaatgta tgtgagtgat tttgctttta cggagatcgg aaggataaaa attattatta
    36181 ataaaggtat tatctgtttc atagcatgtt tcctttcata ataagcgatt atgctcaacc
    36241 gttgtgtgga attaagcgag agtatgtttg acatgattta aaggtttgtt attgagatga
    36301 ttaatgaatt ggagcaaagt aaaagtagtg atctttgcta agattctcgt taacaaccca
    36361 atgaatgttt tcgcgtaatt acgtcgaatt agaaactgat cgacgaactg agagaaaatg
    36421 gtttcaattc gtttacgaac tcttcgataa atagatggct gttttttata attcttttga
    36481 ttgttccgca tcggagtttc gagttgaatg cctttgattt gaaataactc taactgatac
    36541 tcggcatcca gataggcttt atcgccgata agtttacaat tagcatacag tggttcaatg
    36601 tccctaagat actgaatatc agaactctct ggataccgaa ttcccttgca gtgcaagaaa
    36661 taaatagaag aagacctctt aaaaagaacc gaataagaac tatgttgttg ggccgaagta
    36721 cttaggtttc ccccattagc tggaatcgaa aagcctgttt tataagtgtt tt
//
LOCUS       DAOSMP010000019        36163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_1207_length_36163_cov_15.983660, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000019 DAOSMP010000000
VERSION     DAOSMP010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 36163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36163
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1207_length_36163_cov_15.983660"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(269..>3187)
                     /locus_tag="PKX09_04815"
     CDS             complement(269..>3187)
                     /locus_tag="PKX09_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU17178.1"
                     /translation="DSLTSKTLVGANVFLVGTALGSATDIEGNYRIESVPAGNYTLRV
                     TYIGYKNKEYNVVVPEDGTVIQDVKLSPDVIRGQEVIVRGQAIGQAAAINQQITSATI
                     INVVSEEKIQELPDANAAEAIGRLPGVSVLRSGGEANKVILRGLEDKFTNITIDGIKI
                     PPTDETSRGVDLSTLSQSTLAGIELYKAITPDKDGDALAGTINLVTKKAPIARKVKVD
                     FKGIYNDLMKSANQYDLSVQYGERFINNILGVQMTGNMEKRIRSNERITLDYNQNPTQ
                     SDAGYFIDDFILEFTDEIRKRDGFSLLLDINTPDEGSIRFNNVFGRTKRDYFWYSRDY
                     PSNGGGDQSGNPEYDYRDREQEIKTFSSSIHGDNKLFNFGINWGLSFSESESRHTFDY
                     EAYFVEPNGMNPSPKVQSNPKQLIDYAVNDFSAANLYWVYYRPQHNYDKERTLYLNID
                     RQYSFAQTFSGQFKFGGKYKIKDRSNVRSEYFTPYYLGKWQKYEKLPDGTIREKDFSG
                     TYFEEWYNKGVLFIPISEFYSSHESRNIYDSYALDPLIDRDRMRQWWYLNRYGVDVTG
                     NIDEVWTNPLIKYDDYDITERVSAGYLMNTLNIGQAVTFIAGVRVENEDNDYISCYMP
                     NMVSGFPVPANSVLDTTSSSSQTVWLPNFNLAISPFSFMKLRLAAYKALARPDFNMRL
                     ERYIAGRPAEVGTSLQVYVGNPNLKTAQAWNYEINTSFFGDAIGLISVSAYYKEIEDM
                     FHMLNNFNTTAVKDDQGVYQDTLMQRFGIKWPSQMGSAAYNLTLPYNSPKPTKVWGFE
                     FEHQINFTHLPGFLKYFVLSYNASVVRSEAYIWASKIDSVYYDPPGPAPATYKKFYVL
                     TERKQKLEGMPEFFGNVALGYDIKRFSGRISVFHQGEYNISYSASGLSDRVNNPFTRV
                     DLTLKQGLNDNISVFLNVSNLTDIEEGSSIYNRVYDRKLFRNSERYGITADFGINIKL
                     "
     assembly_gap    3188..3287
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3761..4618
                     /locus_tag="PKX09_04820"
     CDS             3761..4618
                     /locus_tag="PKX09_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17179.1"
                     /translation="MVREYKITLIVALLILLIIAIIYNFNLQNQLASYRVIDKKDPIW
                     SEYLQSKQPVLIVLGNHFFFDDYSEKFRSNITIRHGRVNSYEDFNALQSQFPENVLKP
                     SDEPYFPYHSIWSLPPVLSILNSVGQKPILRRSSLITPQTLGEYNIIFLGSIKTLYIL
                     KHTLTQSHFSFEILPHIITYTNGDSCRTFQTNLHSTGLNEDLVLAVKLPGPADNSIFI
                     IASYHSLGAPEVVNQLTQTDSRKQIEQKFIEKYGYMPRYFETLFRVNGIDKTAYSTEM
                     LIFNEITKD"
     gene            complement(4691..6859)
                     /locus_tag="PKX09_04825"
     CDS             complement(4691..6859)
                     /locus_tag="PKX09_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOU17180.1"
                     /translation="MISNLKNIRFSKILLIICLIFNSRLLADDKPKTALVLSGGGARC
                     FAQIGVIKALEEIGFYPDFIVGTSFGALIGALYAGGSTPQEIEEFIANTRWNRVLASQ
                     PFRDIEFVSQKIRTLPELFTLRFDENFNVIFPRNLLSTQALQERIFEMTIYPEYYAGS
                     DFDSLAIPLRIIATDLKTGKMVVLSNGNLARSVTASSAFPIILAPVKYGTYLLADGGL
                     TDNVPIDVALDLGAEFIVAVDVSSKIVPLAENFDVFDIFGQAMNTLAYPSDTKNLELA
                     DVLIRPEIDHITSTDFDSLNSLVESGYRSCQKYWGKIQPYADNICKSPDFLENAINKL
                     NNTSINQIKYSSDQATREYILRREMELKEGDRWNLKLAQRSMKNLYSTGLFKNVYLSL
                     SEVDSSRADLLVEVEEHERTAFSFGARYDSERKASAFISGKYRNLLGRGIDNQVNLII
                     SDLLFRAEWNSRTIRVLSSNFTGYTSLYHLNESIPLYNNDGRRVESGKLYRTGLEANA
                     GIQIKRVGLTAFGLKWENTVADSTIHYIPRVNHDNYSVGSLTLRILVDNVDDPDLPKS
                     GRVNDIRYEHSIERDRLKQFDRISVESVTYETFDEKYTFSTTLHLGYLNKVLNHYEQF
                     RLGGLNSLPGYHQDELWGSMIGVFGLGCRAPLTSGIYYRLSGMVGNVWDGFSDFDWRQ
                     SRAGISLGIVIPTPIGPISADYGIGSHNHRLFYISVGRYF"
     gene            complement(6908..7585)
                     /locus_tag="PKX09_04830"
     CDS             complement(6908..7585)
                     /locus_tag="PKX09_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015946666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOU17181.1"
                     /translation="MLGTLVNVGMVLLGSSLGLLLHTRLPRKFTVIVFQVIGLFTLFL
                     GFSMALKTTNFLIMVFSGALGAVIGELCKIEEAINNLGENLKRRLKTDNDKFAEGLVT
                     AFLMFCMGSMTILGAFEEGMGGEPNLLLAKSLLDGFSSMILSAGLGVGVLLSVIPLFL
                     YQGGLTLLASLLGDVLNPTVINEMTAVGGLLLIGLGLTILEIKTIKVVNMLPGLVVAI
                     ILAGIFL"
     gene            complement(7653..7856)
                     /locus_tag="PKX09_04835"
     CDS             complement(7653..7856)
                     /locus_tag="PKX09_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOU17182.1"
                     /translation="MKKIYRSRTDQKLAGICGGLGEIFNIDSNLIRLALVFATILTGF
                     FPLLITYIVGWIIIPEGPVEGKG"
     gene            complement(8127..9200)
                     /gene="rseP"
                     /locus_tag="PKX09_04840"
     CDS             complement(8127..9200)
                     /gene="rseP"
                     /locus_tag="PKX09_04840"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOU17183.1"
                     /translation="MLYTILAVLIVIGGVVIAHELGHFVFAKLTGMRVEVFSIGFPPK
                     IISKKIGETEYQLSAIPLGGYVKVSGVVDESFDTTAAESNEPWTFAGKKTWQKLLFIS
                     GGVIFNVLFAFVIFSGLTMHEGIYEPVDKPIVESVAPGLPADSLGIKPGDIILAINGT
                     AVESWSSMTELIHSHPYDSILIKWQSGGTIYEKKVKPAANKILKGSELVEVGLIGISP
                     TMNHRPAKFFESLDNGMQNTWYWLKITVISLKMLITGEESIKNIGGPIFIAQLAGETA
                     KFGFGSLLNLIAIISLNLALINILPVPAFDGGHLLITLIEAIKRRPLSVNAKVRIQQI
                     GIAIILILTVLVLFNDISRILKW"
     gene            complement(9219..10385)
                     /locus_tag="PKX09_04845"
     CDS             complement(9219..10385)
                     /locus_tag="PKX09_04845"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOU17184.1"
                     /translation="MAEAGKIRAISVLGSTGSIGVNALKVISASRDRFTVNYLTAGRN
                     GALLIEQAKKFHPKAVAIADESQYRVLKENLSGTNIEILVGKDGILEITSRRDVDLVV
                     NAIVGMAGLEPTYRALTAGRNVALSNKESLVMAGEIIMNLIAQSGLNLFPIDSEHSAI
                     WQCLAGEDKVSVKRLILTGSGGPFRARPQAEFKSITPAEALRHPTWTMGRKITIDSAT
                     MMNKGLEIIEAFWLFGVEPEQIEVLIHPQSVIHSMVEFCDGSIKAQLGLPDMRLPIQY
                     AIGYPERLPIIWEKIEWAKLGPLTFEVPDPVKFPALRLAFEALKRGGTAPAIMNVINE
                     LAVYAFLDEKIGFMEITDIIEKALNEIPVIDKPNIEDILNVEPMAREFFGNFIR"
     gene            complement(10387..11118)
                     /locus_tag="PKX09_04850"
     CDS             complement(10387..11118)
                     /locus_tag="PKX09_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17185.1"
                     /translation="MNIRQFQKFKYLRFVGLFLIAANLSANELDLTALPKNQVRGELQ
                     NILPKLCAGLELPCILLSRPFTPGVLIGARYNCPDLLASPSNLTGLPTCGMAVGATPD
                     IILAGQIGTGRWQEETLNSMGIYLAYVWGKPTAPNQIIGGVNHTKGPVDFHFLDMSLG
                     YLKLFQFNQWLFSVAGTAHFTQVGIHVTDNLNSADNYKTRKNIDFMLVNITLQRNLGE
                     HFQIGTNLTFSPKSVSGGIAIGSYF"
     gene            complement(11121..11894)
                     /gene="lpxA"
                     /locus_tag="PKX09_04855"
     CDS             complement(11121..11894)
                     /gene="lpxA"
                     /locus_tag="PKX09_04855"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009197442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOU17186.1"
                     /translation="MKQVHPTAIIDSQARLGENISIGPYSIISNNVEIGDGCQIGSHV
                     LILPGARIGRDCKIFHHAVISEIPQDLKFAGEETTAEIGERTIIREFVTINRGTSQHW
                     KTVIGSDCLLMAYVHIAHDCIVGNQVIMSNAVNLAGHVEIEDWVIVGGLVGVQQFSRI
                     GQHSIIGGHYRVPKDVPPYIMAAREPLRYEGLNLVGLKRRNFPPETILALKRAYTLIY
                     NSEYNVSDAIKKIEETPELMIPAVRNVIEFIKKSTRGII"
     gene            complement(11898..13295)
                     /locus_tag="PKX09_04860"
     CDS             complement(11898..13295)
                     /locus_tag="PKX09_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOU17187.1"
                     /translation="MSRHQRTIKNEVSISGIGLHTGVKSTITFKPAPENSWIKFVRTD
                     VENCPEIVADINHVTDISRGTTLSQNGVQVHTVEHVLSAVAGLGIDNIIIAINNIEPP
                     VCDGSAIEFVRALKRAEIVEQEAEQDILVIDKILTYSDPKKHVDIHITPAEKFQITFL
                     IDYSEIPAIGTQYSTTYSLEDEFETDYAAARTFCTLSEIEELKNRGLIKGGNVNNAVV
                     FVDRHIEASEVERLRELFGISSDISIGQGGTLNGVELRYYNEAVRHKIVDLIGDFALL
                     GVPIQGHVIAARSGHAHNINFVRKIRKEYEKVMISKKYQKGKVKGALFDISGIERILP
                     HRFPFLMVDKIVDMVPGESVTAIKNVTINEPFFQGHFPVKRVMPGVLVLECLAQTGGF
                     MLLNTIENPEKVLLYFTGLDEVRFRKPVVPGDQIRLEVKMVQSRLSMCKMRGVAYVND
                     EVAVEANMMASLVEL"
     gene            complement(13299..14327)
                     /gene="lpxD"
                     /locus_tag="PKX09_04865"
     CDS             complement(13299..14327)
                     /gene="lpxD"
                     /locus_tag="PKX09_04865"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOU17188.1"
                     /translation="MQLTLGQIARLVNGAVKGDENILINDVAEIQHAQSGTITFLANP
                     KYAKYLASTAATAVIVDENFNGNFPNLILVKNPNLAFSIVLAHFRPELPLPAPMIHPT
                     AVIAKDARIGDDVYIGPFVVIESSAQISDKVTVFGHCYVGQYVNIGSGTVIYPNVSIY
                     HKCIIGQKVRIHAGVVIGSDGFGFVRVENGIEKIPQAGRVVIHDEVEIGANSTIDRGT
                     IGDTVIGRGTKLDNLVQIAHNVRIGEYCFFAAQVGIAGSTIIEDRVNVGGQVGIAGHL
                     HIGTGVMIAAQSGVTKDVPAGTFLFGSPAQERLKAAQELAHIRSLPEIKERLKKVEME
                     LRKGKNIG"
     gene            complement(14330..14851)
                     /locus_tag="PKX09_04870"
     CDS             complement(14330..14851)
                     /locus_tag="PKX09_04870"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOU17189.1"
                     /translation="MKKVVFIILGLLMIGASDLAQAQIKVGYVNSDRILSEFDEAREA
                     QTKLDIEAKKLEDQYRGMLTKLDSLQKDYDRQKMMMSETRRKTKETEISQLQETIQKF
                     QVEKLGPDGEIYKKQAELVTPILEKIKTVIAKVGKDGKYDFIFDTVAGNILYAEPAHD
                     VTDKVIYELKRTQ"
     gene            complement(14885..17254)
                     /gene="bamA"
                     /locus_tag="PKX09_04875"
     CDS             complement(14885..17254)
                     /gene="bamA"
                     /locus_tag="PKX09_04875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOU17190.1"
                     /translation="MIKSKPISLFLLILMLFSLIMAQEEAGIMILGLAVQGHKTISES
                     SIKIQSGLVEGRRVTQEDIAQAIQRLWKLKMFSDIQILLDKETSEGVFLVIKVEEYLR
                     LEKFEVKGNKKIKKSKIEEELNLVPGRVLSANLIAEVKRKVKALYTKEGYLLAQVDAE
                     ITDGTKENSKNLILKIQEGKKVRIDEINFVGNEHFSDSRLRRVMKDTHERNLFILRTG
                     EFDEAKYIEDKEKLRAFYRKEGFRDFKIISDSISYTPNKERMLITINLYEGPRYKYRN
                     INFSGNTVFTTEQLQMLLGIKPGDYYNEELLQKALYDRLNGAYMDRGYLFCQIMPLEV
                     PVAENEIDLTIEITENSIVSIDQININGNTKTNENVIRRELRMFPGDIFSRDALMRSQ
                     NEIYMLNYFANVTPDVVPVSENAVDVELTVEEKSTDQANLSFSISQVYGLVGGGGFQF
                     NNFRGRGQQLAISYQQGTSYSVYSTSVTPYKALSLSFADPWIFDSPNLVGASVSYSER
                     GSGSSYYYPYNLTIYGGSLRWGRRLRWPDNYFRISWSYGAYKKSYTDIGSAYLEEVLE
                     GKDRTTSIGLTQVISRDSRDRPEFPTTGSVFSWVTNLTGGLLGGTEHFTKNTFTLDYY
                     LPTIWKLVLYNHVEFGVINKLKKGTAVPPDERFIMGGSGMIYGTALRGYDDNEVGPTY
                     YYGGYYYPYGGETMLKYSIEYRVPIANNPTIYALAFAEAGNVWKYMRDTDPFDLKRSV
                     GVGIRFYMPALGLLGIDFGYGFDDIDPEGTTGYGKPEGWKTHFIFGTAY"
     gene            complement(17257..18039)
                     /locus_tag="PKX09_04880"
     CDS             complement(17257..18039)
                     /locus_tag="PKX09_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOU17191.1"
                     /translation="MSKEDQEIIKLREEVMSQEVLPRHIAIIMDGNGRWAKQRGLPRV
                     AGHNEGINSVREVTRECGSLGIKVLTLYTFSVENWSRPKSEVSALMTLLLHTIRNEID
                     ELHKNNVRLTLIGHLTDLPDDPLKGLMEGVEKTSQNTGLNLNLALSYGGRVEILDATR
                     AIAKDVLEGRISLDDIDEKLFSDYLYTRGMPDPDLLIRTSGELRLSNFLLWQMAYTEI
                     YVTETLWPDFRNKELLLAIREYQKRERRFGKTSEQITSPSKS"
     gene            18376..19761
                     /locus_tag="PKX09_04885"
     CDS             18376..19761
                     /locus_tag="PKX09_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOU17192.1"
                     /translation="MIKAVNIKSSAENRRVLIADKNPDLCHTLSTFLVKNGCNCQSTN
                     DSATTLRLLEESEFDFLIIDLDLARYDENSILDKAHIISPRTFIILTTTNASVETAVG
                     AFRKGAIDYLIKPLNQRVLLDQINQAIQLREILNENRFLRREIHNQKYPDQIIGESPS
                     IKNIYHLISKVAHSSSNILITGRSGTGKELVARAIHQNSPRANAPFIAINCGAIPETL
                     FESELFGLKKGSFTGAIMDKDGVFRAANGGTLFLDEVGEIPIHTQVKLLRAIESKEIK
                     PIGSTNSLIVDVRILSATQKDLLKEIEEGNFREDLYYRLNIVEIHLPSLAERKEDIPL
                     LVDHFICTYNNELKRKVLGADKDTMKILMDYKWKGEVRELENIIERAVLLCEGDYITP
                     RDLPPGLREGKLTFSDIPDDLKSSVRNFERQHILGILRRVDFDKNRTAELLGIGLSSL
                     YRKIEELGIEL"
     gene            20185..21228
                     /locus_tag="PKX09_04890"
     CDS             20185..21228
                     /locus_tag="PKX09_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17193.1"
                     /translation="MSKKKVNEEKTMMKNLANKIVALFLISLIVFSFSCENNDEKDGG
                     ISIEIVPESPYNSPIWHPDGQFIGLNHTPLKQIIYPDGNESLDYQQVFDYDSTGFWLI
                     NPDGTNMRRIFPYTLHTPSWSPDGEWITFVSDGQIFKMRYTGETFDTTTITQLTSVGR
                     NFFPDWSPDGKWIAHDRTYSYPETADVQGIWLLKSLDGSDRIKISTGRMPDWSPDGNE
                     IIFIDWFDSIQGGIIKYNIQTGNRTLLFDGQGKDIRHAKYSSDGKKIAFTTSPSPTGA
                     NILIMNINDGDFYQLTSDGVDVVYGLPFSWSPEGKKIVYTKYRSTDWTMNNGVLWMID
                     VDSGTETQLTFNP"
     gene            21419..23755
                     /locus_tag="PKX09_04895"
     CDS             21419..23755
                     /locus_tag="PKX09_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOU17194.1"
                     /translation="MKRLRFLDLVIFSISVLFLSGFLNIIPAENDPEKEILVYFTSGV
                     DRAAKGEVSAQIGSMIVKNTLANFNIGEDKITSAFPDFSESDTLIKTLEGRTVGLPNM
                     AKIFRIQVPDDKARESVIDSLKKLPNVLFAEVNGRISVDLLPNDQNFSYQWEFQAGSS
                     VGKICAPEAWDIFQGSSSIKIAIIDEGVDRTHPDLNGKVSGQTTVYGYHGTHVADTAA
                     AQTNNNTGVAGVDWNAQILAKAFNDGTEDGDPYIYQIVTEAVNEGSHVLNNSWKLLNK
                     NGTPGRYSTTVRIAFAYAYKQNRIAVATIGNTGNTTNTVQYPGSFGQGIIAIGATDQS
                     DVHANFSTYGSAIDVAAPGVSILSTFRNGVTFSDPNYEYISGTSMAAPHVTGIASLLK
                     GYNSSLYNDDIEHIIQISADDITTYPATTGWDQYTGYGRVNAKKALDYLCSPYTINHL
                     TASGGYSYSSTGTYTATMLGVEGLSDGVYIVKRYEVRKSVNFGQVFQSSPYVWGRGVV
                     TNGFSAANPNFGMGWCDKVSSTNSSATLKTYIYKVWTISGTYLGWFPTTVSNITWAYT
                     VLGVPVPLSVTISGPTYLSEGETGTFTANPSGGSGTYTNYQWWYRYDGEIEPLEKSVP
                     SAKLPPVGTWYYLEDREGDQSITFGPSYNFSLKCTVTDSKGSTATDVHTAIVEGLTLD
                     LSIPDQLTVSDNSPNPFNLVTTIKYGIPESGLATITIYSITGQKVKTLVNDHLSAGYH
                     QVQWNGTNNFGSEIASGLYILELKSGSDHVMKKLMFAK"
     gene            complement(23856..25805)
                     /locus_tag="PKX09_04900"
     CDS             complement(23856..25805)
                     /locus_tag="PKX09_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17195.1"
                     /translation="MIDIKERTELTNVLVRKFGEENRDRLETGVKQVCDFWRAEDGDL
                     TTLKQFCLDNFISDQAELLKAFEHIEKTFETMSGVLVELNRDLRWYMDVPTGPILPID
                     YLLANFNLGSHVEDDLYATKIAFFVLLNYKVYTLEEILAEGAEWDRQKWAEVRLAQKF
                     TSRVPAQYNQKRYEAYTAADNYITNYNIYMHNLLTEDEQRLFPEELKLIAHWGLRDEL
                     KAQYQLSDGFPRQKLIYEVMLKIINQEIPKIVIDNPAVDWNVAKNTVSGVSSDNSPEP
                     DTRYARWLGVFQGERACDPYYLISRSQIDRRFNEDREIPEDIVRQLFVDVLTAPELKQ
                     TARLISKRLGRELEPFDIWYNQFRAKSQYTEAELDKITQVKFPTAESFKMQLPEILMK
                     LGFDRSTANFLQTKIDVDPARGSGHAMGAARRSDNAHLRTRVTGNGMDYKGYNIALHE
                     LGHCVEQVFSLNKVDHTLLQGVPNTAFTEAFAFVFQKRDLDVLGLSEKDPQSEHLAAL
                     NDLWQTAEIAAVALVDMDAWHWLYEHPEATPKEFEQAVIDIAKRVWNEYYYPVIGKKD
                     SEILAIYSHMIDSGLYLPDYPLGHLISFQIEQYIKGKNLAREMERMCVQGRISPIYWM
                     EGAVGAKLSAEPMISAAYEALKVVQ"
     gene            complement(25928..26380)
                     /locus_tag="PKX09_04905"
     CDS             complement(25928..26380)
                     /locus_tag="PKX09_04905"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOU17196.1"
                     /translation="MSLIKKYFSTSDLDAIKKACECAEKNTAGEIRVSIFEKRPPKTS
                     KMSLPELAYAEFKNLRMDQTRDRTGILLFILLAERQFQILADEGINAKVEQEVWDDIA
                     EQMAEKFKNGEYLSGVVSAVKRIGEILAQWFPRRPDDINELSNEVHIS"
     gene            complement(26419..27171)
                     /locus_tag="PKX09_04910"
     CDS             complement(26419..27171)
                     /locus_tag="PKX09_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HOU17197.1"
                     /translation="MLKIKFRNWRIPALVGLLLFNLTAFGLDVPPLKRHVTDLAGILT
                     PSELNRLEENLIAFENETSNQIAVLIINSLEGEPIEQYSIRVADQWKLGTADRDNGVL
                     LLIAVQDRQMRIEVGYGLEGVLTDLISSQIIRNVIAPEFNKGNYYAGIDQGLRAIMMA
                     TKNEYQADPRQREQYQAERGKSLASLIFFIIFISLFIGGGRRGRRNLLGAIFFSNMFG
                     GGGWRSGGSGGGFGGFSGGGGGFGGGGASGRW"
     gene            complement(27181..27774)
                     /locus_tag="PKX09_04915"
     CDS             complement(27181..27774)
                     /locus_tag="PKX09_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOU17198.1"
                     /translation="MKKGWVILIAVVVVVLLIVGAVINIYNNLVSLEESVNQAWAQVS
                     NQYQRRADLIPNLVETVKGYAAHERETLESVIQARANATSLNVTPEVLNDPEAFAKFQ
                     QVQGELSTALSRLMAVAENYPNLKANENFLALQSQLEGTENRIAVERQRFNQAVQTYN
                     TRIRRFPNNIFAGMFGFEKKVYFEAQEGAEVAPKVEF"
     gene            27985..29067
                     /gene="ychF"
                     /locus_tag="PKX09_04920"
     CDS             27985..29067
                     /gene="ychF"
                     /locus_tag="PKX09_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOU17199.1"
                     /translation="MQIGITGLPYSGKSTLFATLLSRKSESDPFHKKETEKGIVKVPD
                     VRLDRLTEIFNPRRKVPATIEYLKVPGLESQSSAAKGLPPQFLANLKTVDVILLIVRA
                     FDNPLYPHPLNRIDPQGDINFVNSEFLLNDLAIVETRIERLEKQLSKVHNSSYQKELE
                     LLLKCRNFLEDEKPLRSLALSPEEDKILRGFQFLTAKPLIIVLNIDEKEIQQADEITE
                     RYAYLKTVNTTYTVLCAELEKEISELDPAEQPLFLTELGISEPALNRLIRLSYELLGY
                     ISFFTVGEDECRAWTIVNGDNAQKAGRAIHSDIERGFIRAEVVHYTTLLENGNLSTCR
                     AKGLIRSEGRDYIVRDGDVIEFRFNV"
     gene            complement(29190..29888)
                     /locus_tag="PKX09_04925"
     CDS             complement(29190..29888)
                     /locus_tag="PKX09_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOU17200.1"
                     /translation="MFNNKLLVLIDIDGTLIKSGLTPRKALKQAIFEITGCPVTFEDN
                     HLAGLTDPLIITNTLSQLGLGLKDDGLPTKILNRYLEIFSWDYPAAPDKKIYPGAIEL
                     LDYLKTLPVRMGLLTGNAKRGAQIKLAPFDLWRYFDFGVFGDDNANRNELPRLALRKV
                     HELFSESYPPNKVIIIGDTVHDLICAKVNQMQSVIVVRRPEWRLSIEAQKPELLVESF
                     VPLEPVQNWFEKLL"
     gene            30144..>30398
                     /locus_tag="PKX09_04930"
     CDS             30144..>30398
                     /locus_tag="PKX09_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17201.1"
                     /translation="MPKDFELIIFDMDGTLYPVSAEMDSIYPIVALECAAKSVNRQPA
                     EIKADFEQKRAELARYLNGTPTSTLTLMYFYDADMHAFEME"
     assembly_gap    30399..30498
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <30499..30994
                     /locus_tag="PKX09_04935"
     CDS             <30499..30994
                     /locus_tag="PKX09_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOU17202.1"
                     /translation="TLTLMYFYDADMHAFEMEITRRILTKKYVQPDARTIRVIDAIAQ
                     FYPIFLFTTNNRLTAEKILDQIRLSEYFPPERRFTFSDINALPISKTEKINFMKPSTK
                     GFEHIMEKCGANPNRTLMVGDSETMDIEPARRLGMEIYHVTEPALLYALPEWLEIEHN
                     DKKI"
     gene            31287..32165
                     /gene="dapA"
                     /locus_tag="PKX09_04940"
     CDS             31287..32165
                     /gene="dapA"
                     /locus_tag="PKX09_04940"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOU17203.1"
                     /translation="MFKGLGTALITPFTENGEVDYDAFRKLIHYQIDGGADCLIVLGT
                     TGEAPAILSHEREKIVQIAIAETNHRIPVIIGTGSNCLDTVLKYNRMAEELGSDGLLI
                     VTPYYNKGTQESIFQYYRYIAERTKLPIIVYNVPSRTGFNLLPDTMFRIFNACPNIIG
                     IKEANADFGQLTKLMAGRPEGMLVYSGNDDITLPFMALGAEGVISVVGNIIPSEMKQL
                     TVRMAADDLAGAREIQNRYLDLINAMFVEVNPIPVKYAASRLNLCQNVLRLPLIPLSE
                     KNAQLVASLMQAKGLI"
     gene            32162..32809
                     /locus_tag="PKX09_04945"
     CDS             32162..32809
                     /locus_tag="PKX09_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOU17204.1"
                     /translation="MKYGIIGFSGRMGHEIDELFREKGHECVLLIDDKTEKVESVPEV
                     ILDFSLPSGIEKTITNVRKYQCALITGVTGYSAEQLEAIRALGQEVPVVQSFNFSLGI
                     QLMLKMVNLLKEPLKDWDVEIAETHHRFKKDKPSGTAKMLAATIGREVPTSSFRLGNV
                     LGDHSIAFGSLGEIITLSHRTLSRRAFAEGALLSAEFAIRQKSGYFTFTDVLSQD"
     assembly_gap    33126..33225
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            33267..33845
                     /locus_tag="PKX09_04950"
     CDS             33267..33845
                     /locus_tag="PKX09_04950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU17205.1"
                     /translation="MDKNTHELFEVTLSETRSHVLASTTNGIVVEMIERLGEIDFGRI
                     VKISETLVDNFGQNARFNRNMIKKYFNYPETLPFVIRYNGEIEGFIIGVPLEYFDKES
                     WAQCDENLGKNNTIYTYAFIVRKEHRALGLSKMLKRVYQNTLKRRGFLYITGHVMQGV
                     TANFTRDYKIVRQFENWNNTGFTFDYYRSALK"
     gene            complement(33892..34767)
                     /gene="galU"
                     /locus_tag="PKX09_04955"
     CDS             complement(33892..34767)
                     /gene="galU"
                     /locus_tag="PKX09_04955"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HOU17206.1"
                     /translation="MIKKAVIPAAGLGTRFLPATKAQPKEMLPIIDTPTIQYVIQEAI
                     DSGIDDILIISGKGKRAIEDHFDRNTELEHILEQKGESDLATSISRIGEMANIHYIRQ
                     KQLNGLGDAIYYARQHTGDEPFVVMLGDTINDAVIPVTQQLIDIYNQYHQTIIAVERV
                     PLDKIQMYGIVSGDEVSERVYKVNQLIEKPRPTQTRSNLAVAGRYILTPTIYSMLEKV
                     KPGKNNEIQLTDAIQLLLQRESVYAYIFEGKRYDVGNKLDYLKTTVEMALKREEFAGQ
                     FLDFLKSIVAQAEKK"
     gene            complement(34793..35788)
                     /locus_tag="PKX09_04960"
     CDS             complement(34793..35788)
                     /locus_tag="PKX09_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020599588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HOU17207.1"
                     /translation="MIIETHAYARAGLLGNPSDGYFGKTISIIVANFGAHIFLYETPE
                     LRIESSDSDRNVYKNAYDLVERIGSLGYYGGDRLIKAAIRKFFTYCMENNIKLDYRNF
                     SIRYNSSIPRQVGLGGSSAIVTATMRALMKFFKVTIPKELLPSLILAAEVDELGINAG
                     LQDRVIQVYEGCVYMDFNREIMEQKNHGIYIPLDKNKLPKLYLAYKTSLSKVSGRVLN
                     DIKVRYERGDPEVIACLSRIAELAEEGKAAIENGDEATLNRLINENFDNRKKIMTISE
                     SNQEMIDVARECGASAKFAGSGGSIIGIYKDDAMLNRLIVNLKKINARVIKPYIV"
     gene            complement(35981..>36163)
                     /locus_tag="PKX09_04965"
     CDS             complement(35981..>36163)
                     /locus_tag="PKX09_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17208.1"
                     /translation="PFFPLGLRFPAGDVLNAVTISRTNNFYRPDCLLSPFSFSPFLSL
                     FSRPLSSTSLLMTNDR"
BASE COUNT        10632 a         8058 c         6898 g        10275 t
ORIGIN      
        1 atccacccga ggctaatgta tcagccttta tctgattttg cagaaatgtg gcttccgaac
       61 catcatacgg tactaacgta acaatgacat cctgtccata cagtcctgca aataacagaa
      121 aagtaatgat aacaattgcc ccaatttttt cctttaacat aatttctcct ttcatgttaa
      181 ttttttcgct ttaatagatc aataattaac tctttacttt tgattacaac ctcctttcta
      241 aataaaatat tttccggttt cattgctatc aaagttttat gttaatacca aaatcagcag
      301 ttattccgta cctttcacta tttctaaaca atttccgatc atatactcta ttatagattg
      361 aactgccttc ttcaatatcg gttaaattgg atacattaag gaataccgaa atattatcat
      421 ttaatccctg tttcagggtc aggtctacgc gcgtaaatgg attattcacc cgatcactta
      481 aaccagaagc ggaataggaa atattatatt ccccctgatg gaaaaccgaa attcttcccg
      541 aaaacctctt tatatcataa cccaaagcaa cgttaccaaa gaattccggc atcccctcta
      601 atttttgctt tctttctgta agcacataga atttcttata ggttgccggt gcaggccctg
      661 gcggatcata atagactgaa tcaatttttg aagcccaaat ataggcctct gaacgtacca
      721 cagaagcatt atagcttaat acaaaatatt taagaaatcc gggtagatga gtaaaattaa
      781 tctgatgctc gaattcaaat ccccatactt tggtcggttt aggcgaatta taaggaagcg
      841 ttaaattata agccgctgag cccatttgac ttggccattt tatcccaaag cgttgcataa
      901 gggtatcttg atagactcct tgatcatctt tcaccgctgt cgtattgaaa ttattcagca
      961 tgtgaaacat atcttcgatt tctttataat aggccgaaac cgaaattaat ccaatagcat
     1021 cgccaaaaaa tgacgtatta atctcataat tccatgcctg agcggtcttc aaatttggat
     1081 tgccaacata aacttgtaag ctagtcccaa cctcagccgg acgaccggct atatatcttt
     1141 ccagacgcat attaaaatca ggccgagcaa gagctttata tgcggctaac cgtaatttca
     1201 taaaactgaa tggcgaaatt gccagattaa aatttggcaa ccaaacagtt tgggaactac
     1261 tggaagtggt atccaaaacc gagttagctg gcacaggaaa acccgacacc atattaggca
     1321 tataacagga gatataatca ttatcttcat tttctacccg aacaccagca ataaaagtta
     1381 ccgcttgacc aatatttaaa gtattcatta ggtaaccggc actgaccctt tcggtaatat
     1441 cataatcatc atattttatc aatggattag tccatacttc atctatattg ccagtgacat
     1501 caaccccata tcggtttaga taccaccact gccgcatcct atctctatca atcaatggat
     1561 ctaatgcata agaatcataa atatttcttg attcgtggct actatagaat tctgaaatag
     1621 gaataaagag aaccccctta ttgtaccatt cttcaaaata agtaccggag aaatcttttt
     1681 ctctaatcgt accatcgggc agtttttcat acttttgcca tttaccaaga taatatgggg
     1741 taaaatattc acttcttaca tttgatctgt cctttatttt atacttaccg ccgaatttaa
     1801 attgacctga aaaagtctgt gcaaaagaat actgccgatc aatattcaaa tataatgttc
     1861 gttctttatc ataattatgt tgcggacgat aatacaccca gtataaatta gctgccgaaa
     1921 aatcgttgac cgcataatca attaattgct taggattaga ttgaactttt ggtgaaggat
     1981 tcattccatt aggctcgaca aaatatgctt cataatcgaa ggtatgacgc gattccgatt
     2041 cactaaaaga taagccccaa tttatcccaa agttgaatag tttattatca ccgtgaatag
     2101 aactgctaaa agtctttatt tcttgttcgc gatctcgata atcatattcc ggattaccag
     2161 actgatcgcc ccctccattg gaaggataat ccctcgaata ccagaaataa tcccgtttgg
     2221 ttcttccaaa aacattatta aagcgtatcg agccttcgtc cggtgtatta atatcgagca
     2281 aaaggctaaa tccatccctt ttcctaattt catcagtaaa ctcaagtata aaatcatcaa
     2341 taaaataacc tgcgtcggac tgtgtcggat tctgattgta gtctaaggtg attctctcat
     2401 tgctcctgat gcgcttttcc atattaccgg tcatctgcac gcccagaata ttgttaataa
     2461 acctttcacc atattgaaca gataaatcat attgattagc cgacttcatc aaatcgttat
     2521 aaataccttt aaaatctact tttaccttgc gagcaatcgg tgctttttta gtaacaagat
     2581 taatggtccc cgctaaggca tccccgtctt tgtccggagt tatagctttg tataattcaa
     2641 tgcctgccaa tgtactctgt gataaggtgc ttaaatcaac ccctctactt gtttcgtcgg
     2701 taggtggaat tttgattcca tcaatggtaa tgtttgtaaa tttatcttcc aaaccacgta
     2761 aaataacttt attggcttcg cctccagatc gtagaactga cacgcctggt aatctgccaa
     2821 tagcctcggc ggcattggcg tcaggtaatt cctgaatctt ttcctccgat acgacgttga
     2881 ttattgtcgc cgatgtaatc tgctgattga tggctgccgc ttgacctatt gcttgccccc
     2941 gaacgatcac ctcctgcccc cgaatcacat cggggctcaa tttcacgtcc tgaataaccg
     3001 tcccatcttc gggtactaca acgttgtatt ccttattctt ataaccgata tatgtcactc
     3061 gtaatgtata attgccagct ggtactgatt caatgcgata attaccttcg atatccgtgg
     3121 cacttcccag agccgttcct accagaaata cattggctcc gaccaaagtt ttagatgtca
     3181 gcgaatcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatt atttcattaa
     3301 caataaagat aaaaactgtc aataaccttt tttgacattg ttaaatatgt catacaactt
     3361 tttcttggct tttgataaaa atattgttaa agtatcaata gatgattaat gagcaaacac
     3421 gcaaaaaatt attagaaaaa attctgcaga gcaaggagtt cagctcttct aagatttatg
     3481 cttcctactt gacataccta gttgaagcgg ctgaaagggg aaaaaattaa aagaaatttc
     3541 gatcgcaatc gaatttttcg gcaaagatgc caattttaat ccagccgaag ataccatcgt
     3601 ccgtacccat acttataacc tgcgtaagaa actgcagaat tattactacg atgaaggcaa
     3661 ggacgacaaa tatcgtctgc gaattcccaa aggccaatac gatgtcactt tcattccggc
     3721 tttagaaaat acctaccacc ccaagcgaat cttgcgcttt atggttcgtg aatacaaaat
     3781 aaccctaatc gtcgcactac taattttatt aataatcgca ataatttata attttaacct
     3841 tcaaaaccag cttgcgtcct accgggtcat tgacaaaaaa gatccaatct ggagcgagta
     3901 tctccaatcc aagcaaccgg tattgattgt tttgggcaac cattttttct ttgacgatta
     3961 cagtgaaaaa ttcagaagca atataaccat tcggcatgga cgcgtcaatt catacgaaga
     4021 ttttaatgct ctccaaagcc agtttcccga aaatgtactt aaaccttccg atgaacccta
     4081 tttcccctat catagtattt ggagtttgcc gcccgttctc tcaatcttga actctgtcgg
     4141 gcaaaaacca attttaagaa gatcatcctt gattactcct caaacattag gcgaatacaa
     4201 tataattttc ctcggcagta ttaagacact ttatatcctc aaacacactt tgactcaatc
     4261 gcactttagt ttcgagattc tgcctcacat catcacttac accaacggtg attcttgtcg
     4321 caccttccaa accaatttac attccaccgg attgaacgaa gaccttgtgt tagcggttaa
     4381 actacctggc ccggctgata attccatttt tatcatcgct tcctaccatt ccttaggagc
     4441 acccgaagtc gttaatcaac ttactcagac tgattcccgc aaacaaattg aacaaaaatt
     4501 tatcgagaaa tacggctaca tgccgcgcta tttcgagact ttgtttcggg taaatggaat
     4561 agacaaaacc gcttacagca ctgagatgct gatcttcaac gaaatcacca aagattgaac
     4621 ctcaataatc gaattaatta tcaagccagt caagagtgac ttgacgattg atttgattgc
     4681 tttgagatca ttaaaagtaa cgtccgactg aaatatagaa aagccggtga ttatgagaac
     4741 caatgccata atcagccgaa atcggtccaa tcggtgtcgg tatcacaatc ccaagactga
     4801 tacctgctcg agattgtctc cagtcaaaat cactgaaccc atcccataca ttgccaacca
     4861 ttccggacag acgataatag ataccggaag tcaaaggcgc cctacatccc agcccgaaca
     4921 cacctatcat cgaaccccat aattcatcct gatgataacc aggcaacgaa tttaatccac
     4981 caagtcggaa ttgctcgtaa tgattcagca ctttattcaa ataaccaaga tgaagagtag
     5041 tcgaaaaagt atatttttca tcgaaagttt cataggtcac tgattcgacc gatattcgat
     5101 cgaattgctt taagcgatct cgttcgattg aatgttcata ccgaatatca tttactcgac
     5161 cagatttagg taaatcggga tcatcaacat tatcaactaa tatccgcaga gttaaactgc
     5221 caaccgagta gttatcatga ttaacgcggg gaatataatg gatggtagaa tcggcaacag
     5281 tattttccca tttcagacca aaggcggtta gtccgacccg tttgatctgg attccggcat
     5341 tagcctccag tccggttcgg tataatttac cggattcgac gcgtcgccca tcattattat
     5401 aaagggggat actttcattc aaatgataca aagaagtata accggtaaaa ttcgaactaa
     5461 gcacacgaat agttcgggaa ttccattctg cccgaaacaa aaggtcactg ataatcaaat
     5521 taacctgatt gtcaatgcct ctgcccagta aatttcgata ctttccggaa ataaaggcac
     5581 tcgctttacg ctcgctatca taacgcgccc cgaaactgaa tgccgtgcgt tcgtgttctt
     5641 caacttcgac taataaatcc gctcgactgg aatcaacctc agacaaagat aggtaaacat
     5701 ttttaaataa tccggtacta tacaggtttt tcatactgcg ttgtgccagt tttaaattcc
     5761 accggtcacc ttcttttaac tccatttcac gccgtaaaat gtactcgcgg gttgcctgat
     5821 cgctggaata tttaatttga ttgattgaag tattatttaa tttatttatg gcattttcta
     5881 aaaaatccgg tgatttacaa atattgtctg cataaggttg tatttttccc caatatttct
     5941 ggcaactgcg atagccactc tcgactagag aattcagtga atcgaaatcg gtcgaagtaa
     6001 tatggtctat ctccggtcgg atgagaacat cagccaattc gagatttttc gtatcagaag
     6061 gataggctaa agtattcatc gcctgcccaa aaatgtcaaa tacatcgaaa ttttccgcca
     6121 gcggcactat tttactacta acgtccacag ctacaatgaa ttcagcaccc agatcgagtg
     6181 ccacatcaat cggaacattg tcggtcaagc caccatcggc taaaagatat gttccatact
     6241 tgaccggcgc tagaatgatt ggaaaagccg aactggcggt aaccgaccgg gcaagattgc
     6301 cgttactcag aacaaccatt ttgcctgttt ttagatcagt ggcgatgatt cgtaacggga
     6361 ttgccagact atcgaaatcc gatccagcat aatactccgg atagatcgtc atttcaaaga
     6421 tacgttcttg taaagcttgc gttgataaca aattacgggg aaatatcaca ttgaagttct
     6481 catcaaaccg caaggtaaac aactctggta aagtcctaat cttctgggat acaaattcaa
     6541 tatcgcgaaa tggctgagat gccaaaactc ggttccagcg tgtattagca ataaattcct
     6601 cgatttcttg aggggtactc ccaccagcat acaaagcccc aataagagcc ccaaaacttg
     6661 tcccaacgat aaaatcggga taaaagccga tttcctccag tgctttaatc accccaattt
     6721 gggcaaaaca acgtgccccg cccccactta acaccaacgc agttttgggc ttatcatcag
     6781 ctaaaagcct cgaattaaat atcagacaga ttataagtaa gattttacta aacctgatat
     6841 tttttaaatt tgaaatcatt gactgatata aaactcattc gaacggcgct gacaatcaga
     6901 aaatcaacta tagaaaaatg ccagccagaa ttattgctac aaccagaccg ggcaacatat
     6961 taacgacctt gatggtctta atttccagaa tcgtcaaccc gagaccgatc agcaataaac
     7021 ccccgacagc cgtcatttca ttaatcacag ttggatttaa aacatcaccc aagagactag
     7081 ctaaaagcgt caaaccacct tgataaagga acaacgggat cacagataac aaaactccaa
     7141 ctcccagacc ggcagacaaa atcattgaag aaaatccatc cagcagtgat ttcgctaata
     7201 acaaattcgg ttcgccaccc atcccttctt caaaagcgcc taaaatagtc attgaaccca
     7261 tacagaacat tagaaaggca gtaaccaaac cttcagcgaa tttatcatta tcggttttta
     7321 aacgacgctt gagattttca ccgagattat tgattgcctc ctctatttta cataattcac
     7381 cgatcaccgc tcccagcgcg ccactgaaaa ccataatcaa aaaattagta gttttcaacg
     7441 ccatactgaa accgagaaac aaggtaaata acccgatcac ttggaagaca atcactgtaa
     7501 atttgcgcgg caatcgggtg tgcaacagta atcccagact actgcccagt aacaccatcc
     7561 cgacatttac caaagtgccc agcataacga catccgctcc taatagtcga gaatcttcga
     7621 cactcccgcc taatcttagc cttaactcag tcttaacctt tcccttctac cggtccctcc
     7681 ggaataatga tccaccccac aatataggta atcaataatg gaaaaaatcc agttagaatc
     7741 gtggcaaaga ccaacgccag acgaattagg ttcgagtcaa tgttaaaaat ttcacctaac
     7801 ccgccgcaga ttccggctaa cttttggtca gttctcgaac gatagatttt cttcataaac
     7861 atttctccgt taaaatcact ccaattttat tgattatttc ggaagttgga aagcaggatt
     7921 tttaaataat tgggtaattg cgggtgtttt ctaatcatta tcaataaata ttcatttgaa
     7981 tttcgacgaa tattgacaaa atccgtcagc ctgcgggagc ttattcagcg taaaagccag
     8041 ctcggtttca ggattcaggc aagtgccttt gaaaatagga tgcggactga gcttaattac
     8101 ccgctactaa cgacgaactt ccgtttctac cacttaagaa tacgggaaat atcgttaaaa
     8161 agaaccagca ccgttaaaat taagataatc gctataccaa tttgctggat gcgcaccttg
     8221 gcgttaaccg aaagtggacg gcgtttaata gcttcaatta aagtaatcag aagatgcccg
     8281 ccgtcaaaag ccggtactgg taaaatgtta ataagtgcca aattgagact tataatcgca
     8341 atcaggttta gaagggagcc aaatccgaat ttagccgtct ctccagctaa ttgggcaatg
     8401 aaaatcggtc caccgatatt ttttatagat tcttcgccag taatgagcat tttcaaggaa
     8461 attacggtta tcttaagcca gtaccaggta ttttgcatac cattatcaag cgattcgaaa
     8521 aattttgccg ggcggtgatt catcgtcggc gaaattccaa tcagaccaac ttcgactaat
     8581 tcagacccct tcaatatttt attagcggct ggctttacct tcttttcgta aatcgtgccg
     8641 ccactctgcc atttaattaa aatagaatca tagggatgac tgtgaatcaa ctcggtcata
     8701 ctgctccaag attctaccgc tgtgccatta atcgcaagga ttatatcacc gggtttgatt
     8761 cccaacgaat ccgccggtaa accaggagca acgctttcca cgatcggttt atctaccggt
     8821 tcataaattc cctcgtgcat agtcaatccg ctgaaaatca cgaaagcaaa aaggacattg
     8881 aaaattactc cgccggaaat aaaaagtagt ttttgccaag tctttttacc ggcgaatgtc
     8941 caaggctcgt tgctttcggc ggcggtagta tcaaaactct cgtcaactac accggagact
     9001 ttgacatagc cgcccagagg aatcgccgag agctggtatt cggtttcacc gattttctta
     9061 ctaattattt tggggggaaa accgattgaa aagacctcca ctcgcatgcc ggtcaattta
     9121 gcaaaaacaa aatgtcccaa ttcgtgcgca attaccactc cgccaattac aatcaaaacc
     9181 gctaaaatag tataaagcat ctataactct tcctttcttt accttataaa attaccaaaa
     9241 aattcccgtg ccattggttc aacatttaaa atatcttcta tattcggctt atcaataacc
     9301 ggaatctcat tcagcgcttt ttcaattata tcagttattt ccataaatcc gattttctca
     9361 tccagaaaag cataaaccgc tagttcatta atcacattca taatcgccgg tgccgtgccg
     9421 ccacgcttta aagcctcaaa ggcaagccgt aatgccggaa atttcacggg gtccggcacc
     9481 tcgaaagtta acggacctag tttagcccat tcaatttttt cccagataat cggcagacgc
     9541 tcagggtaac cgatagcata ttgaatcggc aagcgcatat cgggtaaacc cagctgggct
     9601 ttgatcgaac catcacaaaa ttccaccatc gaatgaatta ccgattgcgg atggattaaa
     9661 acctcgatct gctccggctc gacgccgaat aaccagaaag cttcgataat ctcaaggcct
     9721 ttattcatca ttgtcgctga atcaatagta atttttctcc ccatagtcca ggtcggatgg
     9781 cgtagagcct cagccggagt aatacttttg aattccgctt gaggacgggc gcggaacggc
     9841 ccaccggatc cggtcaaaat caaacgctta acactgactt tgtcctcacc ggctaaacat
     9901 tgccagatgg cactatgttc gctatcaatc gggaaaagat tcaagccgct ttgcgcaatt
     9961 aggttcataa taatttcacc agccataacc agcgattctt tattagaaag agcaacgtta
    10021 cgaccggcag tcaaagcccg ataggtcggt tctaaacctg ccatcccgac aatagcattc
    10081 accactaaat caacatcccg acgactggtt atctccagta tgccgtcctt cccgactaaa
    10141 atctcaatat tcgtaccgga gaggttttct tttaatactc ggtattgcga ttcatctgcg
    10201 atggcgactg ccttagggtg aaatttctta gcctgttcaa taagtaaagc cccatttcga
    10261 ccggcagtaa ggtaattaac agtaaatcta tcccggctcg cagaaataac ctttagagca
    10321 ttaacgccga tcgaaccggt cgaacccaga actgagattg ccctaatttt acctgcttcc
    10381 gccatcttag aaataacttc ctattgcgat accgcctgaa acacttttcg gcgaaaaagt
    10441 taggttggta ccaatttgaa aatgctcacc caagttacgc tgaagagtaa tatttaccag
    10501 cataaaatca atatttttcc tagttttgta attatcagcc gaattcaagt tgtcagtaac
    10561 gtgaattccg acttgagtaa aatgggctgt gccggcaacg gaaaaaagcc attgattaaa
    10621 ctgaaataat tttaaatatc ccagagacat atctaaaaaa tgaaaatcaa ccgggccttt
    10681 ggtgtggttg actccgccaa taatctgatt cggtgcagtc ggcttccccc agacataagc
    10741 gaggtaaatt cccatggaat ttagagtttc ctcttgccag cggccagtac caatctgccc
    10801 agctaaaata atatcgggag tggcacctac agccatcccg caagttggca gtcccgtcag
    10861 atttgaaggt gaagccagta aatccggaca attatagcga gcgccaatta atacacccgg
    10921 ggtgaaagga cgacttaaaa gaatacaagg taattctaat ccagcacaca atttgggcaa
    10981 aatattttgc aattcgccac gcacctggtt tttgggcagt gctgtcagat ctagctcatt
    11041 ggctgataaa ttggcggcaa ttagaaataa tccaacgaat cgtaaatatt tgaatttttg
    11101 aaattgccga atattcaata tcagataatg ccacgcgtgc tttttttaat gaattcaatg
    11161 acattgcgta ctgccggaat catcagttcc ggtgtttctt caattttttt aatagcatcg
    11221 gagacattgt attcactatt atagatcaag gtataggctc gtttcaaagc caaaatagtt
    11281 tcaggaggaa aattccgtcg ctttaatcca actagattca aaccttcata acggagtggc
    11341 tcacgcgccg ccataatata gggcgggaca tccttaggaa cgcggtaatg tccaccgata
    11401 attgaatgct gaccgatccg gctaaattgc tgaacaccaa ctaaaccgcc tacaataacc
    11461 cagtcctcta tttcgacgtg accggctaaa ttaacagcat tcgacataat gacttgatta
    11521 ccgacaatgc aatcgtgggc gatatggaca tacgccatca aaagacaatc cgagccgata
    11581 actgttttcc aatgttgaga tgtcccacga ttaatagtga caaattcgcg aatgattgtt
    11641 cgctcaccga tttcggcagt ggtttcctcg ccagcaaatt taagatcctg aggaatttcg
    11701 gaaattacgg catgatgaaa gattttacag tcacgaccga ttcgagcgcc gggtaaaatt
    11761 aacacatgac ttccaatctg acagccgtca ccaatttcaa cattgttgct gatgatactg
    11821 tagggaccaa ttgaaatatt ttcaccaagt cgagcttggg aatcaataat agcagtagga
    11881 tgaacctgtt tcatattcta taattccact agcgaagcca tcatatttgc ctcaacagcg
    11941 acttcatcat tgacataagc gacgcctcgc attttacaca ttgataaacg ggattgaacc
    12001 attttaacct caaggcgaat ctggtcgccc ggcactaccg gctttcggaa tctgacctca
    12061 tccaatccgg taaaatataa taagaccttt tcgggatttt caatcgtatt caaaagcata
    12121 aatccgccag tctgcgccag gcattccaga accagcaccc ccggcatgac ccgcttaacc
    12181 ggaaaatgcc cttggaaaaa cggctcatta attgtaacgt ttttaattgc agtgaccgat
    12241 tcaccgggaa ccatatcaac aattttatct accattaaga atggaaaacg atgcggaaga
    12301 atacgctcga tgccgctaat atcaaataaa gcccctttaa ctttgccctt ctggtatttt
    12361 ttactaatca taaccttttc gtattccttg cgtattttcc ggacaaagtt aatgttatgg
    12421 gcgtgaccac ttcgcgcggc gataacgtgt ccctgaatcg ggacacccag cagggcaaaa
    12481 tcgccaatta aatcaacgat tttatgccgc acggcttcgt tataataacg caattctacc
    12541 ccatttagcg taccgccttg accaatggaa atatcactgg aaatcccaaa taattcacga
    12601 agccgttcaa cttcactcgc ctcaatatgc cggtctacaa aaaccacggc gttattgaca
    12661 ttaccgcctt taattaaacc gcgattttta agttcttcaa tttcactcaa agtacaaaaa
    12721 gtgcgagcgg cggcataatc agtctcgaat tcgtcttcga gcgaataagt ggtcgaatac
    12781 tgcgtgccga tagccggaat ttcactgtag tcaattaaaa aggtaatctg aaatttttca
    12841 gcgggtgtga tgtgaatatc aacatgtttt tttggatcag agtaggtcag aatcttatct
    12901 attaccagaa tatcttgctc cgcttcttgt tctacaattt cagcccgttt caaagctcgc
    12961 acaaattcta tagcgctccc atcacagacc ggcggctcga tgttatttat tgcaattatg
    13021 atattatcaa ttcccaaacc agctacagcc gataaaacgt gctcaacggt gtgaacttga
    13081 accccgttct gcgagagagt cgtgccgcgc gaaatatcgg ttacatgatt aatatctgcc
    13141 acaatttcgg gacaattttc gacatcagtt cgtacaaatt taatccagga attctcaggc
    13201 gccggtttaa aagtaatagt actctttacg ccagtatgta gtccaatacc ggaaatcgag
    13261 acttcatttt taatagttcg ctgatggcgt gacataactt atcctatatt ttttcctttt
    13321 cgtaattcca tttctacttt cttaagacgc tcttttattt cgggtaaaga acgaatatgg
    13381 gctaactcct gggcggcttt caaacgttcc tgagccggtg aaccgaatag aaacgttcca
    13441 gctgggacat ctttggtaac acccgattga gccgcaatca taactcccgt gccaatatgc
    13501 aaatgaccgg caatgccaac ctgaccgcca acgtttactc gatcttcaat aatcgtactg
    13561 cccgcaatgc cgacctgagc cgcgaagaaa caatattcgc caattctaac gttatgggca
    13621 atctgcacca gattgtccag tttggtaccg cggccaatga ctgtatcacc gattgtcccc
    13681 cgatctatgg ttgaattagc gccgatctct acctcgtcgt ggattacaac tctaccagct
    13741 tgcggaattt tctcaatgcc attttcaacg cgtacaaagc cgaatccatc acttccgatc
    13801 acaactccag catgaatgcg caccttttga ccaattatac atttatgata gatactgaca
    13861 ttcggataga taaccgttcc gctaccgata tttacatatt gaccaacata acaatgtcca
    13921 aagaccgtga ccttgtcact aatttgcgca ctggattcta tgacaacgaa tggcccaata
    13981 tatacatcat ctccaattct ggcatctttt gcaatcacgg ctgtggggtg aatcatagga
    14041 gctggcaaag gcaactccgg tcgaaaatga gccagaacaa tcgaaaaagc gaggttagga
    14101 ttctttacca atatcaaatt aggaaaattg ccattgaaat tctcatctac tatcacggcc
    14161 gttgcggcgg tgcttgctaa atattttgca tatttcgggt tggctaaaaa agtaatcgta
    14221 ccactctgag cgtgctgaat ttcggcaacg tcattgataa gaatattttc gtcgccttta
    14281 actgcgccgt ttactaaccg ggcaatctgc cctagggtta attgcatacc tattgagtac
    14341 gttttaactc ataaatgact ttgtcggtaa cgtcgtgagc cggttcggca tataaaatat
    14401 tgccagcaac cgtatcaaaa ataaaatcgt acttcccgtc tttccccacc ttggctatga
    14461 cggttttgat tttttccaga atcggagtta ccaattcggc ttgtttcttg taaatctcgc
    14521 catccggtcc gagcttttcg acctgaaatt tttggatcgt ttcctgtaat tggctgatct
    14581 cggtttcctt agtcttgcgt cgcgtttcac tcatcatcat tttctgacga tcgtagtctt
    14641 tctgcagact atccaattta gtaagcatac cgcgatactg gtcctccagt ttcttggctt
    14701 caatgtccag tttagtctgc gcttcacgtg cttcgtcaaa ttcactcaga atccgatcgg
    14761 aattgacata accgacttta atttgagcct gcgctaagtc actggctccg atcatcaaca
    14821 atccaagaat aatgaacact acttttttca cttttcctcc attgttacac atttgttttt
    14881 attgttaata agctgtacca aaaataaaat gggttttcca cccttcaggt ttaccataac
    14941 cggtcgtacc ttcagggtca atatcgtcaa atccataacc aaaatcaata cctaataatc
    15001 cgagcgccgg catataaaag cgtatcccaa cacctaccga ccgtttaagg tcaaaaggat
    15061 cggtatctcg catatatttc cagacattgc cagcttccgc aaatgccaag gcataaatag
    15121 tcggattgtt cgcgatcggc acccgatatt caatagagta tttcagcatt gtttcgccac
    15181 catacggata ataataacca ccgtaataat aggtcgggcc gacttcatta tcgtcataac
    15241 cgcgtagggc tgtaccgtaa atcataccac tgccgcccat tataaatcgc tcgtcgggtg
    15301 gtacagcggt accttttttt aatttattga taactccaaa ttcaacatga ttatacagta
    15361 ccagtttcca aattgtcggt aggtagtaat ccaaggtaaa agtatttttg gtgaaatgct
    15421 cagtcccacc taacaaacca ccggtcaggt ttgttaccca gctgaacaca gaaccagtcg
    15481 ttggaaattc tgggcgatca cgactgtcac gacttattac ctgagtaagt ccgatactcg
    15541 tcgttcgatc tttaccttcc agcacttctt ctaaatacgc actgccaatg tcagtatagc
    15601 tctttttata agccccgtac gaccagctga tccgaaaata attatcaggc cagcgtaaac
    15661 gtctccccca ccgcagagat cctccgtaaa tcgtcaaatt atacggataa tagtaactac
    15721 tgccgcttcc tctttccgaa taggaaacac tagcaccgac taaattgggt gaatcgaaaa
    15781 tccaggggtc agcgaaacta agggagagag ctttatacgg cgtaaccgac gtactataga
    15841 cggagtagct tgttccttgc tgataactga ttgccaactg ttgaccgcgt cctcgaaaat
    15901 tgttgaattg aaatccgcct ccacccacga gaccataaac ctggcttata ctgaaagata
    15961 aatttgcctg atcggtggat ttctcctcca cagtcaactc aacatctact gcgttttcac
    16021 taaccggtac tacatccgga gtcacattcg cgaaataatt aagcatataa atttcatttt
    16081 gactgcgcat taaggcgtcg cgactaaaaa tatcgccggg aaacattctc aattcgcgcc
    16141 gaataacgtt ttcgttggtt ttagtattgc cattaatgtt aatttggtca attgatacga
    16201 tcgaattttc ggttatctca atggtaaggt caatttcatt ctccgcaacc ggaacctcca
    16261 gcggcattat ttgacaaaat aaataaccgc gatccatata tgctccattg aggcgatcat
    16321 aaagtgcttt ctgcaacaat tcctcattat aataatcacc cggtttaatg ccgagtaaca
    16381 tttggagctg ctcagtagtg aataccgtat ttccactaaa atttatattg cgatatttat
    16441 aacgtggtcc ttcataaaga ttgattgtta tcaacatacg ctccttattc ggagtataag
    16501 aaatcgaatc tgaaatgatt ttaaaatccc ggaaaccttc tttgcgataa aaggcgcgca
    16561 atttttcttt atcctcgata tatttggctt catcgaattc cccggtgcgc aaaataaata
    16621 gattgcgttc atgagtatct ttcattaccc gacgtaaacg actatccgaa aaatgctcat
    16681 tgccgacaaa gttgatttca tcaatccgaa cttttttccc ttcctgaatc ttgagtatca
    16741 agtttttact gttctcctta gtaccatcgg tgatttccgc atcaacttga gccaggagat
    16801 aaccttcctt cgtataaaga gccttgactt tgcgcttgac ttctgcgatc aagttagccg
    16861 ataagaccct tcccggaacc aggttaagtt cttcttctat tttactcttc ttgattttct
    16921 tattgccctt gacctcgaac ttctccaaac gcagatattc ttccactttt attaccagaa
    16981 acactccttc actcgtttcc ttgtcgagca aaatttgaat atcggaaaac attttgagct
    17041 tccaaagtcg ttgaatagcc tgagcaatat cttcttgagt aactcttcga ccttcaacta
    17101 atccggactg aattttaatg ctggattccg aaatggtctt atgaccctgc accgccagcc
    17161 cgaggatcat tatcccggct tcttcctgag ccattataag cgagaacagc attaatatca
    17221 atagaaacaa actaatcggc ttagatttta tcatactcaa ctttttgatg gactggtaat
    17281 ttgttcactg gtttttccaa aacgacgttc tcgcttctga tactcacgga tcgccaaaag
    17341 tagttcttta ttacggaaat caggccataa agtctccgta acatatatct cggtataagc
    17401 catttgccag agtaaaaaat tactgaggcg cagttctcca ctcgttctga tcaacaaatc
    17461 aggatcgggc ataccgcgtg tatataaata atcggaaaat agcttctcat caatatcatc
    17521 aaggctgatt cgcccttcca gcacatcttt agcaatggca cgagtggcat ctaatatttc
    17581 aacccgccca ccgtaactca atgccagatt caggttcagt ccggtatttt gactggtctt
    17641 ttcgactcct tccatcaatc ctttcaacgg atcgtccggc aaatccgtaa ggtgcccaat
    17701 taaagtcaga cgcacgttat ttttatgcaa ctcatctatt tcattacgga ttgtgtgcaa
    17761 cagcaaggtc ataagggcag aaacttccga tttggggcgt gaccagttct ccaccgagaa
    17821 ggtatataga gtcagcactt tgatgccaag actaccgcat tcgcgcgtaa cttcccgaac
    17881 cgaattgatt ccttcgttgt gaccggctac ccgcggtagt ccccgctgtt tcgcccagcg
    17941 accgttccca tccataatga tagcaatgtg ccgcggtaag acctcctggc tcattacctc
    18001 ttctcgtaat ttgattattt cctgatcttc tttggacatt tgattcaaag ttaaataaac
    18061 tacagaaatt attatataaa gcctataaag tcaatgaaaa ccatttaaaa ttttggtgaa
    18121 ttaggtaata aagaatatat cggctggtct aaatgaactg ttcaatttcc agattctcga
    18181 ttaagagaaa tgttttaggt ttgtgttttc ttaatagtca taacttcggc tcattttacg
    18241 gcaggcaatt aggattgttc ctcgtaaaat attcatttac ttaaaacgag ccgttaatta
    18301 cttctcattt ccgagattta aaaaatgttt ttgattaaaa cttactctgt tctatattga
    18361 atctaagaaa tttaaatgat caaggcggtc aatataaagt caagtgcaga aaaccgacga
    18421 gtactgatag ccgataaaaa tccggactta tgtcacactt taagtacctt tctggttaaa
    18481 aacgggtgca actgccagtc gacaaacgac agcgctacca ctctgcgtct gctggaagaa
    18541 tcggagttcg actttttgat tattgatctg gatttagcga gatacgatga gaactcaatt
    18601 ctggacaaag ctcatatcat cagtccccgc actttcataa tcttaaccac tacgaatgct
    18661 tccgttgaaa ctgccgttgg cgctttccgt aaaggtgcta ttgattattt gataaaacca
    18721 ctcaatcagc gcgtcctgct cgaccaaatt aaccaagcta ttcaacttag agaaattcta
    18781 aatgaaaacc ggttccttcg cagagaaatt cacaatcaaa aatatcccga ccaaattatc
    18841 ggcgaaagcc cctcaattaa aaacatttat catctaattt ccaaggtcgc ccattcttca
    18901 tctaacattc tgattaccgg ccggagcggt accggaaagg aactggtagc gcgggctatt
    18961 caccaaaaca gtccccgcgc aaacgcaccc tttatcgcca ttaattgcgg cgcaatcccc
    19021 gagacactct tcgaaagcga actgtttggt ttgaaaaaag gctccttcac cggagcaatt
    19081 atggataaag atggtgtttt ccgagccgcg aacggcggga cactatttct cgatgaagtc
    19141 ggcgaaattc caattcatac ccaggttaaa ttactccgcg ccatcgaaag caaagaaatc
    19201 aaacccatcg gcagtacgaa ctcactgatc gtggacgtcc ggattctttc ggcaactcaa
    19261 aaagatttac tcaaggaaat cgaagagggc aacttccgcg aagacctcta ttaccgcttg
    19321 aatatcgtcg aaattcacct gccctcgcta gccgaacgta aagaagatat ccccctactc
    19381 gttgaccatt ttatctgtac ttataataat gaactcaaac gcaaagtatt gggcgccgac
    19441 aaagatacga tgaaaattct aatggattat aaatggaagg gcgaagtacg cgaactcgag
    19501 aatatcatcg agcgtgctgt cctcctctgt gaaggcgatt atatcactcc gcgcgatttg
    19561 ccgcccggtc ttagggaagg aaaattaacc ttctcagata ttccggacga cctcaaatct
    19621 tccgtccgca attttgaacg ccagcatatt ctaggaattc tgcggcgcgt tgatttcgac
    19681 aagaatagaa ctgctgaatt acttggaata ggtctttctt ctctttaccg caagattgaa
    19741 gaactcggca tcgaactata gtttctatct gctcacacgc aagatatatc aaactgttta
    19801 gtagacgtga ttaataagat cgataacaga tcggacacca ttaaatatta taatcagcag
    19861 tcttacagat gacaatcgta ttaccactgc ctaactatca atgcctccgg tgtctgcgct
    19921 ttgcgcaaac ttcgcctgct cataatattt tacaactaat aaatatattc gtggtcattc
    19981 gtgttcattc gcggtcagaa ttctattgac tttcgttaca ggattagcta agctcaaact
    20041 gttatttcaa attgggctac gaagaactgg tattctctcc gcttatcatt tcttcaactg
    20101 agcaggcaac ttatggggta gcagcttttt cttagaatcg gttttgttca gtttgtgatt
    20161 tcgtcggaaa cgattaaatt gaaattgtcg aaaaaaaagg tcaatgaaga gaaaactatg
    20221 atgaaaaatt tggcaaacaa aatagttgcc ctttttctca tttcattgat agtcttttcg
    20281 ttttcttgtg aaaataacga tgaaaaggat ggaggaatta gtattgagat tgttccagaa
    20341 tcaccataca atagtccaat ctggcatcca gacggacaat ttataggctt aaatcacacc
    20401 ccgcttaaac agattattta tcctgatgga aatgaaagtt tggattatca acaagtattt
    20461 gattacgatt cgaccggttt ctggctcatt aatccggatg gaaccaatat gcggaggatt
    20521 tttccatata ccctgcacac accgtcctgg tcgccggatg gggaatggat tacattcgtt
    20581 tccgatggac aaatctttaa aatgagatat accggtgaga catttgacac gactactatc
    20641 acgcaactta catctgtcgg ccgcaacttc tttcccgact ggagtccgga tgggaagtgg
    20701 attgcacatg atagaacgta ttcatatccg gaaaccgctg atgtgcaagg tatatggctg
    20761 ctaaaatctc tagatgggag tgatagaatt aagatttcaa caggaagaat gcccgattgg
    20821 tcacctgatg gaaatgagat tatttttatt gactggtttg atagtataca gggcgggata
    20881 ataaagtata atatacaaac tggaaacaga actctactgt ttgatggtca gggtaaagac
    20941 atacgtcatg ccaaatattc ttccgatgga aaaaagattg cgtttacaac ttctccttct
    21001 ccaaccggtg ctaatatttt gattatgaac ataaatgacg gtgattttta ccagctaact
    21061 tccgacggtg tcgatgtggt ttatggtctc cctttcagct ggtcgccaga aggcaagaag
    21121 atcgtttaca caaaatatag atcaaccgat tggacaatga acaatggtgt tctttggatg
    21181 attgatgtcg attctgggac agaaactcaa cttactttta atccttgatc caaaatgata
    21241 attgatcgga agaaggtgat cgttatgaga gtttaattaa gccccataga gcaagatcct
    21301 ttaatgttac cccgaaaatg agtatcattt tctggaattt tgttctaatg agggttttct
    21361 cttttaaaag atatattcaa ttatctagcc atataacttt tttagaaagg attatgttat
    21421 gaaaagacta cggtttttag atttagtaat tttcagtatt tcggttctgt ttctttccgg
    21481 ctttttgaat ataataccag cagagaacga tccggaaaaa gaaattcttg tttattttac
    21541 ttccggagtt gatcgagcag ccaaaggtga agtatcggcg cagatagggt caatgattgt
    21601 taaaaatact cttgccaatt ttaatattgg cgaagacaag ataacatcgg cttttccgga
    21661 ttttagcgaa tctgatacgt tgataaaaac attagaagga cggacggtag gtttgccaaa
    21721 catggccaaa atttttagaa ttcaagttcc tgatgacaaa gcaagagaaa gtgttattga
    21781 ttcacttaaa aaacttccga acgttctttt tgccgaagta aatggcagga ttagtgtcga
    21841 tttattaccc aatgatcaga acttttctta ccagtgggaa tttcaagcag gtagcagtgt
    21901 agggaaaatt tgtgcgcctg aggcatggga tatttttcag ggaagttcaa gcataaagat
    21961 cgcaataatc gatgaaggtg ttgataggac ccatcctgat ctaaatggga aagtaagcgg
    22021 tcaaacgacg gtgtatggct atcacggaac gcatgttgct gatactgcag cggcacaaac
    22081 aaacaacaac acaggagttg caggagtcga ttggaatgct cagattctgg caaaagcatt
    22141 caatgatggg acggaggacg gagacccgta catttatcaa atcgttactg aagcagttaa
    22201 tgaaggatct catgtactca ataatagttg gaaacttctt aacaagaacg gaacccccgg
    22261 tagatattcc acaacagtac ggattgcatt tgcatatgca tataaacaaa atcgtattgc
    22321 agttgccacc ataggaaata ccggtaatac taccaatacg gtgcagtacc cagggtcatt
    22381 tgggcaagga attatcgcca tcggtgcaac cgatcaaagt gatgtacatg ccaatttttc
    22441 cacctacgga agtgcaattg atgttgctgc tcctggcgtt tcaattcttt ctacctttcg
    22501 gaacggtgta actttttctg atccaaacta cgaatatatt tccggtacat caatggcagc
    22561 tcctcatgtt accggcatag cctcattact taaaggttat aactccagtc tctacaatga
    22621 cgatatcgaa cacattattc aaatttctgc tgatgatata acaacgtacc ctgctacaac
    22681 tggttgggat caatatactg gttacggtcg cgtaaatgcc aaaaaagcat tggattactt
    22741 gtgttcgccc tatactataa atcacctgac agcttcgggc ggttattcgt atagcagtac
    22801 cggtacatat actgcaacga tgctcggtgt cgaaggttta tcggatggcg tttatattgt
    22861 caaacgttat gaagtaagga aatcagtaaa tttcggacaa gtttttcaaa gttcacccta
    22921 tgtttggggg cgtggtgtag tgacaaatgg attttcagcc gctaacccaa atttcggaat
    22981 gggctggtgt gataaggtat cttctacaaa ctctagtgct acgctaaaaa cttatattta
    23041 taaggtgtgg acaatttctg ggacatatct cggctggttc ccaactaccg tatcaaacat
    23101 tacctgggca tatactgttc tcggagttcc cgttccatta tcggtaacca tctccggacc
    23161 aacctattta tcggagggtg aaaccggcac ttttacggct aatccatcgg gcggcagtgg
    23221 tacatataca aattatcagt ggtggtatag atatgacgga gagattgaac ctttggaaaa
    23281 atcagtgccg agcgcgaaac ttcctccggt tggtacatgg tattacttgg aagacagaga
    23341 aggagatcag agtattactt ttgggccatc atataatttt tctctgaaat gcacagtaac
    23401 cgattcgaaa ggcagtactg caactgatgt tcacacggct attgtggaag ggcttacctt
    23461 agacctctcc attccggatc aattaaccgt tagcgataat tctcccaacc cttttaatct
    23521 tgttacaact ataaaatatg ggataccgga atccggatta gcaacaatta cgatctattc
    23581 tattaccggt caaaaagtca aaacgttagt aaacgatcat ctttccgccg gttatcacca
    23641 ggtgcaatgg aatggaacta ataatttcgg cagtgaaatt gcatcaggat tgtacatatt
    23701 ggaactaaaa tcaggttccg accacgtgat gaaaaagcta atgtttgcaa aatagcactt
    23761 tagaaatttt atcaggtcat tcaaaactgt atcgcaaaaa aaagtcccgc cggaagcaat
    23821 caccaagctg ggcttttcaa ttatctcaaa taatcttact gtactacttt aagggcttca
    23881 tacgcggcag agatcattgg ttcagcggag agttttgccc cgactgcacc ttccatccaa
    23941 taaatcggac taatgcgccc ctggacgcac atccgctcca tttctctagc gagatttttg
    24001 cctttgatat attgctcgat ctggaaggaa atcaaatgac ccagcgggta atcgggtagg
    24061 tagaggccac tatcaatcat atgtgaataa attgccagga tttctgagtc tttcttacca
    24121 attactggat aataatattc attccagact ctcttagcaa tatcaatcac agcctgctcg
    24181 aattccttcg gtgtagcttc aggatgttca tagagccaat gccaagcatc catatcaacc
    24241 agcgctaccg ccgcaatttc agccgtctgc catagatcat taagagcggc gaggtgctca
    24301 ctctgaggat ctttctccga taagcccaac acatccaagt cgcgcttctg gaaaacgaaa
    24361 gcaaaggctt ccgtaaaagc cgtatttggt actccctgca ataaagtatg atcaacttta
    24421 ttgagcgaaa aaacctgctc gacgcaatgt ccaagctcat ggagagcaat attataaccc
    24481 ttgtagtcca taccatttcc cgtgactctc gtgcgcagat gcgcattatc cgaacgacga
    24541 gctgctccca tagcatgacc ggaaccgcgg gcagggtcaa cgtcaatctt agtttgcaaa
    24601 aaattagccg tactccggtc gaagccgagt ttcatcagga tttccggcaa ttgcatttta
    24661 aaagattcag ccgttggaaa tttcacctga gtaattttat caagttcggc ttccgtgtat
    24721 tgagacttag ctcgaaattg gttataccag atatcgaatg gttccagctc gcggccaagt
    24781 cgtttagaga tcaatcgtgc agtctgttta agttccggcg cggtcaatac gtcaacaaaa
    24841 agctgtcgga caatgtcctc gggaatttcg cggtcctcat tgaaacgacg atcaatttgc
    24901 gatcgggaaa ttaggtaata cgggtcgcag gctctttcgc cttggaaaac gcccaaccac
    24961 ctggcgtagc gcgtatccgg ctcaggtgag ttgtcagagc tgacaccgct aaccgtattt
    25021 ttagcgacat tccaatccac cgccggattg tctatcacaa tctttggaat ttcctgattg
    25081 atgattttca acattacttc ataaatcaat ttttgccgag ggaaaccgtc agatagctga
    25141 tactgcgcct ttaattcatc acgcaaaccc caatgcgcaa ttaatttcag ctcctcagga
    25201 aaaagtcgct gttcatcctc cgtcagcaga ttgtgcatat aaatattata attcgtgata
    25261 taattatcag cggcagtgta ggcttcatag cgtttctgat tatattgtgc cggaacgcgc
    25321 gaagtgaatt tctgcgctag acggacttct gcccacttct gccgatccca ctcggcacct
    25381 tcagcaagaa tctcttccag agtataaact ttatagttca gtaaaacaaa aaacgcaatt
    25441 ttggtagcgt ataaatcatc ttcgacatga ctgccgagat tgaaattagc cagtaaataa
    25501 tcaatcggta gaataggacc ggtcggcaca tccatatacc atctcaaatc acgattcaat
    25561 tctaccagaa caccgctcat cgtttcgaaa gttttttcga tatgttcaaa agctttcaat
    25621 agctcagcct gatcggaaat aaaattatcc agacaaaatt gcttcagagt ggtgaggtcg
    25681 ccatcttcag cccgccagaa atcgcaaact tgcttgacgc cggtttcaag gcggtcgcga
    25741 ttttcctctc cgaattttcg gactaaaacg tttgtcaact cggtgcgctc tttgatatcg
    25801 atcattttag cctccggttt tttctcaccg caattatgca aaaagcttaa tgaaaaggat
    25861 aaaaataaac ctaaaaatat gaggtttaaa tctgataatt tcattctaat tcctcttatt
    25921 tttaaactta agagatatga acttcattac tgagttcgtt gatatcgtcg ggcctgcgcg
    25981 gaaaccactg ggcaagaatc tcgccaattc gttttactgc ggatacaact cccgaaagat
    26041 attcgccgtt tttaaatttc tcagccatct gttcggcaat atcatcccag acttcttgct
    26101 cgactttagc attaataccc tcgtcggcta aaatctggaa ctggcgctcg gctaataaaa
    26161 taaacagcaa aattccggta cggtcgcgag tctgatccat tcttaggttt ttaaattctg
    26221 cataagcgag ttccggtagg ctcatctttg aagtttttgg cggccttttt tcaaaaatgg
    26281 agactcttat ttcaccggca gtattttttt cggcacattc gcaagccttt ttgatagcat
    26341 ctaaatcact cgtcgagaaa tactttttaa tcaggctcat ttattcctca ttaaaatgaa
    26401 aatcagttaa tcaccagttt accatcttcc tgaagcgcct ccaccgccga atccaccacc
    26461 gccgccggaa aaaccaccaa atccaccgcc ggagccaccg gaacgccagc caccgccgcc
    26521 aaacatattc gaaaaaaata tcgccccgag cagattgcgt cttccccggc gcccaccacc
    26581 gataaagagt gaaataaata tgataaaaaa gatcaatgag gcaagtgatt taccgcgctc
    26641 ggcttgatac tgttcccgct gtcgtggatc ggcttggtat tcatttttgg tagccatcat
    26701 aattgcccgt aagccttgat ctattccggc gtaataattt cctttgttga attctggtgc
    26761 tataacgttg cggataattt gagaactgat aagatcggtc aggacgccct ccagaccgta
    26821 accgacttcg atccgcatct ggcgatcttg aactgcaatt agcaacaaaa cgccattgtc
    26881 cctatcggca gtaccgagtt tccactgatc ggcgacccga atcgaatatt gttcgatagg
    26941 ttcgccttct aaactattaa taatcaaaac cgcaatctgg ttggaagttt cattctcaaa
    27001 ggcgatcagg ttttcttcga ggcgatttaa ctcagaagga gtaagaattc cagctaggtc
    27061 agttacatgc cgcttcaaag gcggtacatc cagtccgaaa gccgtcaggt tgaaaagcaa
    27121 tagtccaacg agcgctggga ttcgccagtt gcggaatttt attttaagca taatctgtta
    27181 ttaaaactcg acttttgggg caacttcggc gccttcctgg gcttcaaagt acactttttt
    27241 ctcaaatcca aacattccgg caaaaatatt gttcgggaaa cggcggatgc gagtattgta
    27301 agtctgcacc gcctggttga agcgttgtct ttcaactgca atacggttct cagtgccttc
    27361 caattgcgat tggagtgcca agaaattttc gttggctttt aggtttggat aattttcagc
    27421 cacggccatt aagcgcgaaa gggctgtgct aagctcgccc tgtacttgct ggaatttggc
    27481 aaaagcttcc ggatcgttca gcacttccgg ggtaacgttc aagctggtag cgttagcgcg
    27541 cgcctgaatg acactttcta aggtttcgcg ctcatgagcg gcatagcctt tgactgtctc
    27601 gactaaattg ggaatcagat cagcccgccg ctgatattga ttacttacct gagcccaggc
    27661 ttgatttaca ctttcttcca gcgaaactaa gttgttgtaa atattgatta ctgcgccgac
    27721 tattagtaga acgacgacga ccacagcaat taagataacc caaccttttt tcatattttt
    27781 aactcctttt caaattactc ggtaaatcta agaaattttt acacattcga caatcaccag
    27841 gctaaaaaag aaatcggaaa ttaattataa aatgaccaga ttgaccttga cgtgttgatt
    27901 aaagggtagt cgtccaggtt tggtgatctg actattcgaa gttaaattcc gccgtttttt
    27961 aatggtattt tcattggagt ttagatgcaa atcggcatta ccggactgcc ttattccggt
    28021 aaatcaaccc tgttcgctac tttattaagt cgcaaaagcg aatccgatcc ttttcacaaa
    28081 aaagaaaccg aaaaaggcat cgtaaaagtg ccggatgtcc gactcgaccg tctaacggaa
    28141 attttcaacc cgcgccgtaa agttccggca actattgaat atctgaaagt ccccgggctg
    28201 gaaagtcaat catctgccgc taaaggctta ccgcctcagt ttctcgctaa tttgaaaacc
    28261 gtggacgtga ttctgctaat cgtcagagct ttcgacaatc cgctttaccc acatccgctg
    28321 aatcgcattg acccccaggg cgatattaat tttgtcaata gcgaatttct gctaaatgac
    28381 ctggctatcg tcgaaacccg cattgaacgt ctcgagaaac agctcagcaa agtgcataat
    28441 tcatcctatc agaaagaatt ggaactgctc ctcaaatgcc gcaattttct cgaagatgaa
    28501 aaaccactgc gttctctggc gctttcaccg gaagaggaca aaatcttgcg cggattccaa
    28561 ttcttaactg ctaaaccatt aatcattgtc cttaatatag acgagaaaga aatccagcaa
    28621 gcagatgaaa tcaccgaacg ttacgcttac ttgaagactg tcaacactac ttataccgtt
    28681 ttatgtgcgg aactggaaaa ggaaatttct gaactcgatc cggccgagca accgctcttt
    28741 ttaaccgaac tgggaattag cgaaccagcc ctcaatcgtc tgatccgttt atcttatgaa
    28801 ttgctaggct atatttcatt ttttaccgtc ggtgaggacg aatgccgcgc ctggacaatc
    28861 gtcaacggcg acaacgctca gaaagccgga cgggctattc attccgacat cgaacgcggc
    28921 ttcatccgcg ccgaagttgt gcattatact acattattgg aaaacggcaa tctttctact
    28981 tgtcgagcta agggtttgat tcgctcggaa ggacgcgatt atatcgtcag agatggggat
    29041 gtgatcgaat tccgatttaa tgtttaaaga aatttacctt atttatccgc ggagagcgcc
    29101 aagaacgcag agtattagtg aaaattggta aattaaatct gtatttaata atcatgttaa
    29161 tcttgtttat cctgttaaaa taaatccagt cacaacagtt tttcaaacca gttttgcacc
    29221 ggttccagcg gaacgaagct ttcgactagt aattcgggtt tttgagcttc gattgacaac
    29281 cgccactccg gacgccgcac aacaattacc gattgcattt gattgacctt ggcacagatg
    29341 aggtcatgta ccgtatcgcc aatgataatc actttgttcg gcggatagct ctcgctaaat
    29401 aactcatgaa cctttctcag cgcaaggcgc ggcagttcat tacggttggc gttgtcatcg
    29461 ccaaaaacgc caaagtcaaa gtagcgccat agatcaaaag gtgcgagctt aatttgcgca
    29521 ccgcgcttag cgttgccggt taataatccc atccgaaccg gtagagtctt gagataatcc
    29581 agtagttcaa tcgcacctgg ataaattttt ttatccggag cggctggata atcccagcta
    29641 aaaatttcaa gatagcggtt taaaattttt gttggcaaac catcatcctt aagcccaagg
    29701 ccgagttggc tgagagtatt agtaataatt aggggatcgg tcaaacccgc taaatggtta
    29761 tcttcaaagg taaccggaca accagtaatc tcgaaaatag cctgtttgag cgctttgcga
    29821 ggagtcagcc cagacttgat aagggtaccg tcaatatcaa ttagaacgag aagtttatta
    29881 ttgaacatca tttaatttaa atcgcactca gttattagaa tctaccttta atttacaatg
    29941 aaaattaatt tgaatgttct gatgaattcc gaacgacgcg taattttcag attccacaat
    30001 taaaaatcaa ataaccgtga caaaatctga atatttccgg aaaatcgcaa gttttttcgc
    30061 tcagcttata cctaaaaaat aattggaggt ctcggctcaa aatgataatt tccacgccga
    30121 ttttcctatg attgagctat cgaatgccaa aagattttga actgattatt tttgatatgg
    30181 acgggactct ttatcccgtt tcggcggaaa tggattctat ttatccgatc gtggctttgg
    30241 aatgtgccgc taaatcggtc aatcgtcagc cagcggaaat taaagctgat tttgaacaaa
    30301 aacgggcaga attggcgcgt tatttaaatg gcactcctac cagcactctg actttaatgt
    30361 atttctatga cgccgatatg cacgcttttg aaatggaann nnnnnnnnnn nnnnnnnnnn
    30421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30481 nnnnnnnnnn nnnnnnnnca ctctgacttt aatgtatttc tatgacgccg atatgcacgc
    30541 ttttgaaatg gaaattaccc gacgcatttt gactaagaaa tatgttcagc cggatgcacg
    30601 cacgattcgg gtaatcgatg ctattgcgca attctatccg atttttctct ttacaaccaa
    30661 taaccggctt acagctgaaa aaattctcga ccagatacgg ctatctgaat attttccgcc
    30721 ggaacgacgg ttcactttca gcgatattaa tgctctgccg attagcaaga cagaaaaaat
    30781 caattttatg aaaccgtcaa ccaaaggatt tgagcatatc atggaaaaat gtggcgctaa
    30841 tcccaatcgg acgcttatgg tcggcgattc cgaaactatg gacatcgagc ctgcccgacg
    30901 gctcgggatg gaaatctatc acgttaccga accggcgtta ctctatgctt tgccggaatg
    30961 gttggaaatt gagcacaatg ataaaaaaat ttaggttatt aaatatattg gcaaaaaaat
    31021 ctctaaaaat tacttgcatc ttagttaaat ctattttatt ttttatttgt gttgtgatta
    31081 attaaaagaa aattgaattc aaatgattta tcgaggcatt ataattaatt caaaatcggc
    31141 gttctcacgc aactatgtta aagttgccaa gatcgctaag aaacccgatt tgtaaccccc
    31201 taatagaatg aatgtatttt tatacatagg gggtccaagt agtagacccc ctttttgttt
    31261 ttataaatct aaaaagtgag gaagttatgt ttaaaggatt aggaacagcg ttgattacgc
    31321 ctttcacgga aaatggagag gttgattacg atgcgttccg taaattaatt cattatcaga
    31381 ttgatggcgg agcggactgt ctaatagtgc tgggaactac tggcgaagca ccggctattc
    31441 tgagccacga acgcgaaaag attgtccaga ttgctatcgc ggaaaccaat catcgcattc
    31501 cggtaatcat tgggaccggc tcgaattgtc tcgataccgt tttgaagtat aatcgtatgg
    31561 cagaagaatt gggaagcgat ggattattga tcgttacgcc ttattataac aaaggcactc
    31621 aagaatcaat ttttcaatat taccgataca ttgccgagcg taccaaatta ccgatcatcg
    31681 tctataatgt tccgtcgcgc accggtttca atctcctgcc ggacacgatg tttcggattt
    31741 tcaatgcttg tcccaatatc atcggaatta aagaagccaa cgccgatttt ggtcaattga
    31801 cgaagttaat ggcagggcgc ccagaaggta tgctggttta ttccgggaat gacgacatta
    31861 cactgccgtt tatggcgctc ggagccgaag gcgtcatttc tgtcgttggc aatatcattc
    31921 catccgaaat gaagcagttg acagtacgga tggcggctga tgatctggct ggcgcacgtg
    31981 aaattcaaaa tcgctatctg gatttgatca atgccatgtt tgtcgaagta aacccgatac
    32041 cggttaaata tgcggcatcg cgcctgaatc tgtgccaaaa cgttctgcga ttaccgttaa
    32101 tcccgttatc ggagaaaaat gcgcaattag tagcgagctt aatgcaagca aaaggattaa
    32161 tatgaaatac ggaataattg gctttagcgg ccgaatgggt cacgaaatag atgaactttt
    32221 ccgtgaaaaa ggccatgagt gtgttctcct aattgatgat aagacagaaa aagtcgaatc
    32281 ggtgccagag gtaattctcg acttctcact gccatccggt attgaaaaaa caataaccaa
    32341 cgtcagaaaa tatcaatgtg cgttgatcac cggagttacc ggttatagcg cggaacaatt
    32401 ggaggcaatt cgtgctcttg gtcaagaagt accggtagtg cagtctttca atttttctct
    32461 cggcatccag ctaatgttga aaatggtaaa cctgctgaag gaaccgctca aagactggga
    32521 cgtggaaatt gccgaaactc accaccgctt taaaaaggat aaacccagcg gtaccgccaa
    32581 aatgctcgcc gcaactatcg gcagagaggt gccaaccagc tctttccgct tgggaaacgt
    32641 tcttggtgac cattcgatcg cctttggcag tttaggagaa atcattacgt tgtcccatcg
    32701 cacgctctcg cggcgcgcat ttgcggaagg cgcattgcta tcagccgaat ttgccattcg
    32761 tcagaaatca ggttatttca catttactga cgttttgtct caggattgat gcgagatcgg
    32821 ccgggttcca attggacttt atcaaaccga aaatattcaa ttgctaaact attttcatag
    32881 tcgaaaatta ttttcatggc gaagaatacg atatatggaa ttacagtcag tacaatcgta
    32941 atgagaaaac gtacgtaagt tttcaatgga ctaaatcata atgaacaacg ctcaatacaa
    33001 ttggatcatc attgtacaat ggaaaaagta agtgattgac tattcagatt taggcaatta
    33061 attcattctc gccattgatt atttcaattt ttctgtctat aattccgccg tatggataaa
    33121 aatacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngccat tgattatttc
    33241 aatttttctg tctataattc cgccgtatgg ataaaaatac ccacgaatta tttgaagtca
    33301 cactttctga aacccgcagt catgtattag cctccactac caacggaatt gtggtcgaaa
    33361 tgatagagcg tctcggtgaa attgacttcg gtcggattgt caaaatttct gaaacactgg
    33421 tggacaattt cggccagaat gctcgcttta accgcaatat gatcaagaaa tatttcaatt
    33481 atccggaaac cctacctttc gtgattcgct ataatggtga aatcgaaggt tttattatcg
    33541 gggtaccgct tgaatatttc gataaagaaa gctgggcgca atgcgacgaa aatctcggca
    33601 agaataacac gatttacact tatgctttta tcgtgcgtaa agaacatcgg gcgctggggt
    33661 tatccaaaat gctcaagcgg gtttaccaaa ataccttgaa acgtcgcggt tttctataca
    33721 tcaccggcca cgtaatgcag ggggtgacgg ctaattttac gcgcgattat aaaatcgtcc
    33781 ggcaattcga aaattggaac aataccggct ttacttttga ttactaccgt tcggcactaa
    33841 aataatcttt tgatgtttta attgaaatat tttaaagcct ttgaatagat tttatttctt
    33901 ttctgcctga gcaacgatag atttgagaaa atccagaaac tgcccggcaa attcctcgcg
    33961 cttgagtgcc atctcgactg ttgttttcaa ataatccagc ttgttaccga cgtcgtagcg
    34021 tttgccttca aaaatatagg catagaccga ctcgcgctgt aataaaagtt ggattgcgtc
    34081 cgtcagctga atttcgttgt ttttacctgg cttaactttt tcgagcattg agtatatggt
    34141 aggagttaaa atataccggc cggctactgc tagattggaa cgggtttgag tgggacgcgg
    34201 tttttcgatc agctgattga ctttgtaaac acgctcactg acttcgtctc cggaaacaat
    34261 accatacatt tggattttat caagcggtac tctttcgact gcgatgatag tctggtgata
    34321 ctgattgtaa atatcaatca gttgttgagt taccggaatg acagcatcgt taatagtatc
    34381 gccgagcatc actacgaaag gctcatcgcc ggtatgttgt ctggcgtaat agattgcgtc
    34441 tccaagacca ttgagttgct tctgccggat ataatgaata ttagccattt ctccgatcct
    34501 gctgatactg gtagccaaat cactctctcc tttttgttcg agaatgtgct ccaattccgt
    34561 attgcgatca aaatgatctt caatggcacg tttgcctttg ccggaaataa ttagaatgtc
    34621 atcaattccg gaatctatgg cttcctggat aacgtattga attgtgggag tatcgattat
    34681 cggcaacatt tccttgggtt gagctttggt agctggtaaa aagcgcgttc ctaatccagc
    34741 ggctggtatg actgcttttt tgatcatttt atccatttcc tcctaaataa tattaaacaa
    34801 tgtagggttt aatcacccga gcattaattt tctttagatt aacgattaag cggttgagca
    34861 tcgcatcatc tttataaatc ccgataatgg aaccgcctga gccggcaaat ttagcggaag
    34921 cgccgcattc cctggcaaca tcaatcattt cctgattgct ttcactaata gtcataattt
    34981 tcttacgatt atcgaaattt tcattgatca gccgattcag agttgcctca tcaccgttct
    35041 caatagccgc tttcccctcc tcggctaact cagcaatacg gcttaaacag gcgatgactt
    35101 ccggatcgcc gcgttcataa cggactttaa tatcattcaa cacccgacct gatactttac
    35161 tcagcgaagt tttataagcc aggtaaagtt tgggcaattt atttttatcc aacggaatat
    35221 aaattccgtg atttttttgc tccataattt cgcgattgaa atccatatag acacagcctt
    35281 cgtaaacctg gatgacgcga tcctgcaaac cggcattgat gcccagttca tccacctctg
    35341 ccgccagaat caggctcggt aataattcct taggaatcgt tactttaaaa aatttcatta
    35401 acgctcgcat tgtagccgtc acaatcgcac tcgaaccacc cagaccaacc tgacgcggaa
    35461 tcgaagaatt ataacgaatg gaaaaattgc ggtagtccag cttaatatta ttctccatac
    35521 aataagtgaa aaatttccga attgcggctt tgattaggcg atcaccacca taataaccca
    35581 acgaaccgat ccgttcaacc agatcataag cgtttttata gacgtttcgg tcagaatcgg
    35641 acgattcgat tcttaattcc ggtgtttcgt ataggaaaat gtgcgccccg aaattggcga
    35701 cgatgatcga gatggttttc ccaaaatagc cgtcggaagg attgccaagc aatccagctc
    35761 tggcataagc atgagtttcg ataatcatta tgttaaaccg atttttatca tatttagatt
    35821 cacagcgata atatacttag tcggaagttt gtaagtcaag ttaacaattg ggattataaa
    35881 aaaaacgacg aacaaagatg aatgcatgga tatggtcatt ggtcattggt aaagaggctg
    35941 agaaaagaga ttgagactga gaaaagtcac tggtcattag tcatcggtca ttagtcatta
    36001 gtaaagaggt tgaggaaaga ggtcgagaaa agaggctgag aaagggagaa aaggagaaag
    36061 gagacaggag acaatcagga cgataaaaat tattcgttct agatatcgtt acggcattaa
    36121 gaacatctcc ggctgggaag cgaagtccca gcggaaaaaa tgg
//
LOCUS       DAOSMP010000020        35526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_1233_length_35526_cov_16.297313, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000020 DAOSMP010000000
VERSION     DAOSMP010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 35526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35526
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1233_length_35526_cov_16.297313"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(357..2237)
                     /gene="ftsH"
                     /locus_tag="PKX09_04970"
     CDS             complement(357..2237)
                     /gene="ftsH"
                     /locus_tag="PKX09_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOU17209.1"
                     /translation="MKKNEKFSLIYFWLITLMIIFTQLLFFPDRPANVRLSYRDFLNA
                     LDSGKVARIVIFEDRIVGEFKSEDINEEQALTHAPTAPWRLRVPSIEKQVARQFIVSR
                     IPELPDPELLAKLQASGVNFEGRYESNSLRNFFLNWVFPIIFFIGLWGVIFRRMGSSN
                     PMLDFGRNKAKIYAEKPENQVKFSDVAGVDEAVEEVKEVVDFLKNPKKYTRLGGKLPK
                     GLLLVGPPGTGKTLLARAVAGEAGVPFFNLSGSDFVEMFVGLGAARVRDLFKQAKAAA
                     PCIIFIDEIDAIGKSRGNALTYGGGYDERENTLNQLLVEMDGFDPGVGVIIIGATNRP
                     EVLDPALLRPGRFDRQVLVDRPDMKGREAIFKVHTRNLKLSSNVDLKQLAAQTPGFAG
                     AEIANVCNEAALLAARNNRDEVTQSDFEAAIERLVAGLEKKNKIINEKERKIVAYHES
                     GHAIVGYFTPGAEQVQKVSIVPRGIGALGYTLQMPLEDRYLMTKSEIIGKIKGLLGGR
                     AAEEIVFNEVSTGASNDLERVAQLVKNMIVVYGMSEKLPNYSLVNRSTPGFLNQSAGI
                     ERRSEHLERLIDEEVKEVIDKCYRETKEMLIEKRELLDKMANTLLEKEVLEKEEIEAI
                     LS"
     gene            2598..3041
                     /locus_tag="PKX09_04975"
     CDS             2598..3041
                     /locus_tag="PKX09_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17210.1"
                     /translation="MLKKKNAILIFWLLASFAFAEEKGGVIPCLASFFIGPRVGLEMN
                     EGKQIETIEWIHFGSNFVPYVGLVVKAYTSYEIGGKTNGAGGFFASYCIGPRVGAELN
                     YRKIRTLEWLRLVPCINIYPFIKIPLEAYQGKTMTEIEQKEGLRK"
     gene            3176..3517
                     /gene="yidD"
                     /locus_tag="PKX09_04980"
     CDS             3176..3517
                     /gene="yidD"
                     /locus_tag="PKX09_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOU17211.1"
                     /translation="MLSQEHQKWTDYWEIPLNLPEKPGEIFSTNLFSSSAIAWIKIYQ
                     KVIASQDLPSCVFHPSCSRFALTSIQEFGIVKGALLASDRLMRCNPFAMRYYPFDGEK
                     LADPPQKYRLK"
     gene            3530..4366
                     /locus_tag="PKX09_04985"
     CDS             3530..4366
                     /locus_tag="PKX09_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17212.1"
                     /translation="MKKSILFLLILSISTTANAQIESSLFEPAHLKEFGLYLFQQKDY
                     LRAAMELERYRYFAKSGDDSITFVIGLCHQFRQRYDFAAATFRELAEDDSSKFKESAR
                     LAMLYNYTKLEDWPKILGLSYQNDAEFYFYYLASVKTAPTTITTSFFQNVNDESLRNQ
                     LKALEQDRNKIHPKSPFLAGTLSTLIPGLGKVYINRSGDALFSFGMINLFALLTYKAF
                     QAELPVTGVLSGGLTLSLYLGTIYGSYVGAELYNRNLDADWQSKMTELNPVDRHPYWS
                     TW"
     gene            4360..5193
                     /locus_tag="PKX09_04990"
     CDS             4360..5193
                     /locus_tag="PKX09_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17213.1"
                     /translation="MVKRIILLLLAIFSVIFSTENLGDYYNAKQHYFEAITEYKRQLF
                     FGQYESQDEILLKMAKVYHAVRQDLVAEEILLQAISNDETSAFDWECSLELARLHWDN
                     YNYFEMRNVLDFISINSEQPIQDTINYIKAWTYFYEAKWGEGLALLDSVHFIDSKSLQ
                     KEIAGVHKIPQKSRTLALVMSNIIPGTGHLYAHDYQNALYSSLLVGGTMVSIISNVID
                     RAYFVALIKYLFIYSRYNNGTLVNLAKKVDRDNIDAIGNYLKTVSQKYPAPLELLECL
                     R"
     gene            complement(5461..6201)
                     /locus_tag="PKX09_04995"
     CDS             complement(5461..6201)
                     /locus_tag="PKX09_04995"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOU17214.1"
                     /translation="MKEFKIGCCGFPIEKEQYYREFNCIEINITFYQLPQIVTAEKWR
                     LAAEKQNPKFEFVIKAWQIITHPAGSFTYRRLREKIGAKENYGLFKDTDEVFTAWERT
                     REFALKLGCTKILFQCPPSFTPDEKYLLNLYNFFEKIRKDSEDFEFILEVRNPKWDKK
                     TIQKVCRELNLIHCVDPLYQPSYYGDYRYYRLHGLHIGNRLDYNYTFSDEELEKVLAL
                     CDQELNYIMFNNFTMYYDALRLMELVGR"
     gene            complement(6409..6729)
                     /gene="trxA"
                     /locus_tag="PKX09_05000"
                     /pseudo
     CDS             complement(6409..6729)
                     /gene="trxA"
                     /locus_tag="PKX09_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997735.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
     gene            complement(6822..8063)
                     /locus_tag="PKX09_05005"
     CDS             complement(6822..8063)
                     /locus_tag="PKX09_05005"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU17215.1"
                     /translation="MKNIMIITRWEYLTRLRSKGFIISTIIIPLIMVASMFIPSVVLN
                     SDDHEVRLIAVVDETGQFAEKLEQKLADKYTLADQTPKYQVIVIKNGDPVRMKREAAE
                     LLNSSVISAYLVIPEVVLDSSRVFYYSLNVGNFKDQSELNLVINEIISEYRLVKAGLD
                     PALIQDLMKNVEFQTIKVASSGQETLGDETLSFIIPLIYVLMLFFTIFMSSQVLMRSV
                     LAERTNRLIEILLSSVSPTELMSGKILGLGLLGLTQLVLYLIVGIMISNFKSLELFSS
                     INVILFLVYFVLGYLLYAAIFAAIGSLFDNEQDAQQAVSIFSLISVVPIFLASYVIAN
                     PQSLTTIILSFIPIITPYFMILRMGVMMPPLWEILTSLGILVVSVWLAMLAAGKIFRV
                     AILIYGKRSTFPEIWQWVRAR"
     gene            complement(8060..8950)
                     /locus_tag="PKX09_05010"
     CDS             complement(8060..8950)
                     /locus_tag="PKX09_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU17216.1"
                     /translation="MLQLKQVRKVFNKTVAVDSLDLNIPTGSIFGLLGPNGAGKTTTI
                     RMIMNIIQPDAGEILYNGKHLEQSDLLKVGYLPEERGLYQKMKLKETVVYLARLKGLS
                     SAEANQSVDEYLRRFDLSAYSHRRIQELSKGNQQKVQFIVSVIHKPELLILDEPFAGL
                     DPVNQLLLKEIIAELQGRGATVIFSTHQMEQVEKLCDRICLINKGKPILNGDLRQIKK
                     EYGQPMLLIDYDGQADTLADLNLPDMQITDNRLTCRLTGNLNELLLSVMQRVQVTRFE
                     LQEPSLEQIFIELVRGAENS"
     gene            complement(8983..10200)
                     /gene="tyrS"
                     /locus_tag="PKX09_05015"
     CDS             complement(8983..10200)
                     /gene="tyrS"
                     /locus_tag="PKX09_05015"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOU17217.1"
                     /translation="MFPPVKEQLEIIRRGVEELISEEEMVRKLENSIKTNQPLRIKQG
                     FDPTAPDIHLGHTVGIRKLRQFQELGHTIVLIIGDYTGLVGDPSGKNETRPRLQYETL
                     IKNAETYENQFFKILDRSKTEIHYNGEWFAKMKFDEIMNLAAKFTVARILERDDFTKR
                     YKEGNPISLHEFFYPLMQGYDSVAIKADVELGATEQKFNLLTARDVQREYGQEPQVIL
                     TLPVLEGIDGGQRMSKSLGNYIGIDDEPDEMYGKIMSIPDNLILKYFTLVTDYSLARL
                     EEVKRRLNDGQTNPMVLKKQLARTIIKMYHSESAARQAEQNFEQIFSKKEIPDDIEEI
                     IIPSSDLPKPLVKLLTECGAVSSNGEARRLIHQGGVRINNEKINDINYSFQNQGEYIT
                     KVGKRRFFKFIVK"
     gene            complement(10203..10664)
                     /gene="smpB"
                     /locus_tag="PKX09_05020"
     CDS             complement(10203..10664)
                     /gene="smpB"
                     /locus_tag="PKX09_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOU17218.1"
                     /translation="MPKDDVKIITTNKKAFHDYFILDKIEAGIELKGTEVKSVRAGHI
                     NLKDSYAKIIDRELYLLKCHIAPYDFGGYDNHNPERDRRLLLHRNEIRRLQREVENKG
                     VTLIPLRVFITDKGKVKVEIGLAKGKRQYDKRNALAEKEARREVERLRKGK"
     gene            10751..10834
                     /locus_tag="PKX09_05025"
     tRNA            10751..10834
                     /locus_tag="PKX09_05025"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10785..10787,aa:Leu,seq:cag)
     gene            complement(11112..11315)
                     /locus_tag="PKX09_05030"
     CDS             complement(11112..11315)
                     /locus_tag="PKX09_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17219.1"
                     /translation="MRRRNLGILMISLMILFCIGVLFVGQEYSSSSKLMTVRVEGLPD
                     EQSGKNMQDLMKMTEGIESFIFD"
     gene            complement(11666..11971)
                     /locus_tag="PKX09_05035"
     CDS             complement(11666..11971)
                     /locus_tag="PKX09_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17220.1"
                     /translation="MWLEHSGDGGSTWTFGNNGQPLDGTAGGKCPSIAFTTHNQGYVV
                     DNYIGVVWQEKYGTKYKIKGKIFNQYGDVNSAPSPYYEVTTLFTEPNDTYNAVNNRL"
     gene            complement(12132..12650)
                     /locus_tag="PKX09_05040"
     CDS             complement(12132..12650)
                     /locus_tag="PKX09_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17221.1"
                     /translation="MLVILISGCEKECPTELTEEGLNLITNSSFEINGNKSIDGWTLH
                     SNFLDVGFSNDVPPFGGNWSVVLYGGDRIVPMYLETKIAAPLGCNRYRLSIWAKFKDP
                     QGYGKGYVNFNKNRSNGKYFTIQDSIWNYYELIDTLTTTNGDSLYVIISIMPFIYPSQ
                     IYYDLCQLELIQ"
     gene            complement(12831..13070)
                     /locus_tag="PKX09_05045"
     CDS             complement(12831..13070)
                     /locus_tag="PKX09_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17222.1"
                     /translation="MHNLKLTFDRIKPIIKESIETAPEYGYCAAQNSHYFGYKFHGVC
                     SLNGVITSFDLSKANHALQSEELSKTTGEPCSLIW"
     gene            complement(13155..13589)
                     /locus_tag="PKX09_05050"
     CDS             complement(13155..13589)
                     /locus_tag="PKX09_05050"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOU17223.1"
                     /translation="MHNINYILMTLLLALGACSDSTSPNQNIPANLKNIWWEVQSFQS
                     VTGEIITLAPTEDYFLLFGDSTIYGKSDTLCGNNYHAPYWIGGNDSLSIGLIISTEAW
                     CRSNYWKYLSALQNATSYKINDNTLTIFFNDSKRILYTRRVE"
     gene            complement(14029..17751)
                     /locus_tag="PKX09_05055"
     CDS             complement(14029..17751)
                     /locus_tag="PKX09_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOU17224.1"
                     /translation="MSKRIFSCLWIVIFLAELAFSGGIVPPKQDPNNPEYVKGEILVK
                     FKDETQIRRSLAKGVQQTGITAVDQLNQKYQVEEMEKVFKDAKPHLTKPLFKDHQGKV
                     HEVQNLDKIYKLKYKAEIDPVEISKEYANDNNIEYAEPNYIAQICVEPNDPYFDQQWG
                     LHNTGQYHLEDADIDAPEAWEVEKGDTSVIIGIIDTGVDWGHPDLVDKVVSTGYDFVN
                     NDNDAMDDNGHGSHVAGIAAATTDNSIGIAGVAWNCKILPVKVMQSNGTGSYSDVANG
                     VTYAANNGAKIINLSLGGYAESYLLRDALENAYTTAVIVASAGNNGTSAPFYPAAWSF
                     VIGVGASDVVYKLVPEGENVVVVWYETKAEFSNFGINADLYAPGVSILSTVPLFHPHR
                     YDYASWNGTSMASPYVAGTAALIRSKYPDWSNEMIHGQLVYTTDPINGYNRLNAHRAL
                     IEIGEPQLSLYSYTITDTLPGDDRDRIADAGETVEMVFDIQNTWAQAYNVEAILRPHS
                     YEDTCFVTILDSLANFGGGVSAYAHVNNGSNPFLFYVKPSTPNNTDVYFDYEITCDGG
                     YSWNGTFFIKTQRGIEISGIIREDRTLTNDYLYIVTGNVLVDEGVTLTIEPGTRIQFC
                     PEKYLRIDGCLVAIGTEDSMIVFTSNAQNPAPGDWYGIRFTDKSRDAIFDANGNYLSG
                     SIVNYVEIEFGSGISISDASPYIEYNFIHDNYGVQGTGVESSGGITLVSSSTSRVEHN
                     LIRNNSNSRGGGIYLYNCNGIPIIAYNIIEDNMSESGGGIAVYTSSAIITQNIIRGNR
                     GSYSGGSGIYGSGRNLYIFKNNIYNNYSSYIPSDAGGVYLSQNVNFINNNIMFNSCNG
                     VVFGYNFEGRMDSNNIFGNDEYEIKMDDSKNVNSLNNYWFTSSTDSIDAWIWDYYDDF
                     SLGKVFYEPFETTPVVTAPGFLYQVQLNPPSPIGCEVDTFNLIFSKPMDISVQPYVTF
                     GVCDPWTQHIVEGDWIDSTHWQGTYTFNLMTGDGINRLRVTTAQDCEGMEIPKDTRFA
                     FVVDAAGAASEGFIAQAGNGRVDLSWHKPEMIDLMGYNLYRYQSLTDTTFSDTTLVNT
                     SMISDSSYTDLTVQNGTTYFYMFTAISTDFNESSYSQPVSATPRATGIEYLMSQIPKE
                     YSLRQNYPNPFNPITNIIYGMPERSYVTIKIYDLLGREVITLVNENQEAKYYKILWDA
                     KDKFGNNVSSGIYFCRIIAESDNRIFSKTNKLLLLR"
     gene            complement(17932..19023)
                     /locus_tag="PKX09_05060"
     CDS             complement(17932..19023)
                     /locus_tag="PKX09_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU17225.1"
                     /translation="MIEINYYEAYKEMKNSYDIRFKMVHYALDNGVSKAANQFGTTRK
                     TVRKWLKRYQSEGTLGLVDHSRRPHHSPFKITVQEEQQILAIRNRQPYLGPIRIKAEH
                     GIRSSPATIHRVIKNAGLIRQRKRKSQVKRDLRNVKSKMRLFEKIQIDLKELSDIPRY
                     YPYLWHNYPKYQITARDVRTGLIYIGYAYEKTTTNVAIFTYLLGCHLLSCGVDLNQTV
                     WQSDNGSEFIGPWNQRNKQTLYEKIVGALNSESIQIPVGRKTYNSDVEAAHRLIEDEF
                     YDMEDYHNIGELLSKAFTYCVYFNYQRKFRYKYMKTPIEILNEYNPPNINPETIGIFP
                     PIITDHYLNIITKSGYHVPRSDSEMKLFH"
     gene            complement(19257..21029)
                     /locus_tag="PKX09_05065"
     CDS             complement(19257..21029)
                     /locus_tag="PKX09_05065"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU17226.1"
                     /translation="MLLIKDKQVFAWAMYDWANSAFATTVMAGFFPVFFKSYWSAGTD
                     VNVSTAMLGYANSIGSLIVALISPILGAIADQSSSKKRFLIFFAYLGVLTTTCLFMVD
                     YGQWLWAVIFYVAGSLGFAGANTFYDSLLPEVAPSHKIDYVSAKGYALGYLGGGLLFL
                     INVLWYLSPATFGFPVEQQAKVYQALSQNATVIQIEPGKDFSIPKKHSIGKAVVTSEI
                     KLPITIVDYHSDDSVWISVPVPPDIDQDLIVSAISFSQFKSGEIVAKKPGKNEYLIGN
                     LNQKLSPEDSAVIIIRNPITYQSFRDQRIEGVEGLSNHCGRITITTDYLTPAQENLPI
                     RVSFLSVGLWWGIFTIPLILYVKEKRREPRPNRRSTYIRQGFKQLSQTFQQVRHLKVV
                     MIFLVAYWLYIDGVDTIIRMAVDYGLSIGFPSSSLIVALLIVQFVGFPAALVFGKLAE
                     KWNPKKVIFATIAVYLGVTAYSVLMKDVKEFYILAIVIGCVQGGIQALSRSFYLRLIP
                     KEQSAEFYGFFNMIGKFSVIFGPFLIATVGLLARRAGFSADLASRIGIGSIGILFLVG
                     AILLCFVNEEKGRVEAEKLRILNK"
     gene            complement(21164..22399)
                     /locus_tag="PKX09_05070"
     CDS             complement(21164..22399)
                     /locus_tag="PKX09_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOU17227.1"
                     /translation="MKRDLLLIVALISLISIEGTMAKERTGWGWGGVPAINYNADEGF
                     GYGMIFDIFNYAKGGYSPYYFKIDNQIFFTTGGKQDHTIFFDSPYILGNGFRISVRTR
                     YKKEKFQPYYGLGNDSKFDPSYIEVDEDGNSLDSLHGKHYYTFNSEQIIFYTNILKAL
                     KYREDGRPLISVLGGLGFYQLGCGADDNGGIATKFEEDLNAGILKKRDTDKSFNDYLK
                     FGLVYDSRDNEPAPNRGVWTDIVGEWYTGLIGSDNDFLRLTLTDRRYFTLRKNLVYAN
                     RIIVENIFGEAPLLLYYPLGGSFRIDEGVGGYRSIRGAYKNRYVGSAKFIMNMELRYR
                     FYEFQFAGQDFYLAANAFFDFGRVWHENDLKGGFTNLHTGKGGGVHIGWNENFIVYAE
                     MAFNEEAGSQLYIDIGFLF"
     gene            complement(22471..>22857)
                     /locus_tag="PKX09_05075"
     CDS             complement(22471..>22857)
                     /locus_tag="PKX09_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOU17228.1"
                     /translation="SYNMGKIKLMMDFNYYNNGAVDWDGDEKNFDNTTEFGLGAEYAL
                     TEKLKVSLGGQYSMMGAKPESQSDMDFNLDAFTIGGGVEYQISPKVKLNVGALDAFYI
                     EAENASGIEKYNQTNYLVAFGVQVKL"
     assembly_gap    22858..22957
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22958..24134)
                     /locus_tag="PKX09_05080"
     CDS             complement(<22958..24134)
                     /locus_tag="PKX09_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17229.1"
                     /translation="MKIRRVLLCVVLLVSGTVLAGGLVTNTNQSAAWVRTLNRNASTD
                     VDAVYYNPAGLTKLFEDGLFLDVSNQTIWQTKTVTNDTPTLNNHEFVGKVFAPVFPSA
                     YVAYKMDKLVLSGGFMPIGGGGSAKFEKGLPSFEVPVSALKTALAGVGVTDYKLDVSF
                     EGSSIYYGVQGGVSYKINEIFSVFGGARYVMASNSYTGYLKNIQVLRSGNWEKPSTYF
                     FGVATAATGAANSLSTLVAAAPNITLQDAKAAGYLTEEQVAQLESGLIAAGINPTGYT
                     VAQVQNAYTTVAGTMTSYGTNLDAQTADKEVDAAQSGSGVAGIFGINISPNENLNIGI
                     RYETITKMELENDTKKDDTGLFPDSAKYGADIPSQLAVGVSYNMGKIKLMMDFNYYNN
                     "
     gene            complement(24310..25629)
                     /locus_tag="PKX09_05085"
     CDS             complement(24310..25629)
                     /locus_tag="PKX09_05085"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOU17230.1"
                     /translation="MKIIWDKRRSFFGELILLLLVFSEINGQTAKLRHSTVITLPVDE
                     ETQDFKIGLDLLIDDNFHALDNYKFMVIADRNSFAQGNQYIFDIVNIRLGTRCRSCVQ
                     VLDDRSLLTGSGFRLSQSQLYGITKYYILNSERDQLSANMLNGAEMIVLDLQDFGLRE
                     SEALRVLIEALKFSAKQKIPIIVLDRPNPLRGVEIGGPMSIQSQFAADFSLPARHGMT
                     LGELALLMNEENWLNDGDKSQLTVIKMANYYRDMPWQQTGLKWSFADESVQDEETAVL
                     YAGLYFFQFTNISVGTGTPHPFHVVGAPWMSANELVNALAKYDLAGVEISQVKFIPRR
                     ELYASEMPLYLDTECLGINLKVSDLNKFDAYKFSATLISVISKLYPKHFKWKKTTEAD
                     AYFGNADFRTWVNIGADLNPMLADWEAKASRFQKLRLKYCLYPVQKN"
     gene            complement(25637..27310)
                     /locus_tag="PKX09_05090"
     CDS             complement(25637..27310)
                     /locus_tag="PKX09_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU17231.1"
                     /translation="MASEPATVKNRSPWLWVPSLYFAEGIPYVIAMTVSVIMYKTMGV
                     SNAALAFWTSVLYLPWVIKPIWSPFVDVISTKRNWVLITQFILGCCFIGVALVVRLPL
                     WFALTLIVLWIIALASATHDIAADGFYMIGLDAHQQTWFVGIRSTFYRMAMIVGQGLI
                     VMLTGYIMSKTGLKPVSITAMAVPPNEIMQVLDENNLPAIKASEQPNIVIIPAEVKVP
                     IYNPAEPDGDSANVFICLSAPPKKEETVVVNFGRKKGSKDIWLTSAGRLEFTAENWNK
                     PVKVTLKVDHRLREPVVSQFEATAGDIPFSWAVSFGILGVFFLLICLYHKLMLPTGLE
                     TVRVKSQELKSSWLKVFSSFFQKKGIVSALSFLLFYRLAESQLVKMASPFLLDSQEAG
                     GLALTTTQVGFVYGTVGIICLTIGGILGGIVAARNGLKRWVLWMALAINIPDLVYVYL
                     SYVQPDNFMIVTICVAIEQFGYGFGFTGYMLFMLYLAEGEYKTSHYAIGTAFMALGMM
                     LPGMISGKLQDLMGYRTFFVYIMFCTIPSFLVASLVKIDPDFGKKKIEN"
     gene            complement(27330..28679)
                     /locus_tag="PKX09_05095"
     CDS             complement(27330..28679)
                     /locus_tag="PKX09_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOU17232.1"
                     /translation="MLEPNVIPTEEPILRVGIFLPIDKCQRIALVIPQSPVYELIPES
                     GPRQILKGGEQIEFILAANQIGIQINGVLQNSQPSWKIIPRGGEIRIANKAGIKVKNV
                     VAGRGFHWEKYIDVYLPGSIEISRMNDNLIMINVLPLEQYLMCVATSEMGAACPLSVI
                     KSQTIIARSWLLANVEMKHRNLGMDVCNDDCCQRYQGTTFLSEHSIKGALETTGQVLI
                     YGEKICDTRYHKNCGGVTESFENVWNGSAVPYLDSVLDIPPDCQHPALPLDTEAKVRN
                     WIENPPLAFCSPSIVPEKELKKYLGNVDKEGHYFRWHFRMTQKEITALFNLKLNLDAA
                     AIKSIEPLKRGKSGRLTSLAINYRDKANVDKQIVINDQYAIREAFHELFLYSSAFVVD
                     VEEGSENVPVAFVLKGAGWGHGVGYCQIGALGMALKGYSTLEILEHYYPGVKLHQAY"
     gene            complement(28661..29908)
                     /locus_tag="PKX09_05100"
     CDS             complement(28661..29908)
                     /locus_tag="PKX09_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17233.1"
                     /translation="MANCKKDIADDEFLLIGIDGGATKVSGWSVKTANHGTAFALGNF
                     HATKSYNEIPGYIHNFQPVDLNRQIAEWEAGKIVPTNAELVQSSCYIKAASWCIEQIT
                     KRSGTNRVLIGIGMPGIKTRDGRGIAVLKNGPRITNYAVRLENEITLAGIELVQPINQ
                     IGSDAYYCGIGEEYAGEGSFRNVRNSYYLGGGTGAADALKLDGKVIPLDETKGWFVKT
                     WEMKCPDGYSVEKYVSSSGIQSIYGELIGKSPEELTRNGIFPPQIRRRALEGEKAAAI
                     TMQKVAHFLALLFYERITSIYAGWQGIFDFVNPDRPRPQTENNYKKALFDNLVIGQRL
                     GELLCEAQGDEVLWLPFVNELTQLIINSSVLDEVAKEHYCPQKHLNLARIQISPLREA
                     PALGAAIDAYLTWKERKLCLNRM"
     gene            complement(29911..31494)
                     /locus_tag="PKX09_05105"
     CDS             complement(29911..31494)
                     /locus_tag="PKX09_05105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU17234.1"
                     /translation="MKSTNTISAVSVILHYIPEMADQFARTLTSVFNQSCLPLEVLVL
                     CQADQPVPEFDCCSLPLEIIREAGNLSRTLNRVMETAKGKYLLFIDNSVAEVILKHSL
                     LDVSFLLAEKYPDLGMLYGDYDLVNNGKIQEIRLLKHHSGRLRDNQDYGKVFLFSKAA
                     LEAVGGFDQSLKYNALYDIRLKLSEKYKLVHIANKIDGSLYRVVSGGVKHNVFDYLLV
                     NKEAQLEAEVVLTNHLKRIGAYLAPDQGYHLRPDLNEKYDCLASVIIPVYHRPEFIGT
                     ALNSVFSQSIKDLEIIVVVNGGPLDPTVTEVRRYMAGGDRYDAQQPPVRLIVADINNI
                     GLSLNLGLKIARGRYYVQLDSDDRLKSDAIEKIVAVFNSDERIGMVIGSYEVWEKKPS
                     GELVRMEEIPIVKHEEWTDANGRNNLLRINGAGAPRAIPIKIIREMGYFGMNDEPFAC
                     NYGEDYDLVLKISEQYRIGRVWQPIYEVVRHTGGTDHSIDQNTIDRNDEAKDYMRLKA
                     VHRRKKINEITNRNLEKSL"
     gene            31826..32143
                     /locus_tag="PKX09_05110"
     CDS             31826..32143
                     /locus_tag="PKX09_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17235.1"
                     /translation="MNKTIKISLLLLLIVGLLFSAYRLGSKKDNTTILSVSTTVQDFN
                     SVLANSLEQLEKTANEAEFGKIILNVDDTAIIVTVKMKDLRKLTRTEIPFDQFIRTYL
                     KFS"
     gene            complement(32362..32715)
                     /gene="trxA"
                     /locus_tag="PKX09_05115"
     CDS             complement(32362..32715)
                     /gene="trxA"
                     /locus_tag="PKX09_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOU17236.1"
                     /translation="MSEKLTKARFLKEIFDFENESTWKYKGELPSVIDFYADWCYPCK
                     MVAPVLEELSKKYAGKVNIYKVNTDEEGELAEYFSIRSIPSLLFIPKNGKPQMLVGAL
                     PKNQLDQAIQDILIK"
     gene            32900..33511
                     /locus_tag="PKX09_05120"
     CDS             32900..33511
                     /locus_tag="PKX09_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17237.1"
                     /translation="MLKTDKKQLDKLLELQGEVKGVVFQTDGKYVLQKEGEEGLKKLE
                     KRVKELGYPIDYRKKGEALDLSPIGLRVISLLIIQDTFNWQDSEIRNMGYAAPGFSFV
                     VKLFMHFFISLKKSANEYPSYWTLHYTIGNLEVISLDEKSKDVTIHLNDFKVHPIMCL
                     YLEGYFERIYEFAVGKGKGNCTETKCMFEGDPYHEYVFKMKRQ"
     gene            33515..>35526
                     /locus_tag="PKX09_05125"
     CDS             33515..>35526
                     /locus_tag="PKX09_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU17238.1"
                     /translation="MVLTAETRELEKYIEDIWQFLPIPVCYINAVHLIIAVGANFEKF
                     SGYDSEELVGMELETLFADQQEIKKIEEEILNSGFVSNRVITFITKEKNEVPVLLSAT
                     ARKDEEDNIFGYFMSMLDITKRRQYEQALKESERRFSAFMETLPHTAFIKTGDHKYLY
                     INNAMKKRFGIEEWVGKTTFDLFPKEIAEKLVKADNISLVKGYHIEESTVPDKSGNEC
                     IWETYTFRIERENQEPLIGGFSMDITEKKESEKKLRENEEFLNSIIDSIQDGISILKP
                     DLTINFTNDVMKKWYAENLPLEGKKCYECYHNANAPCDPCPTLRCIQSGKTEWNIVPG
                     LPGSPIKWIELFSYPIKDPDTGKVTGVAEFVRDITSKRQAEEALRQSENKYRLLFQTS
                     PDLIAQVDKKGRFITANPAMADSFGILSEELIGKTFFEVMPPDIAQHRLEMVRKALKE
                     DRIQNFEDERDGKYYYNLFVPVKISEQEEATVQVIARDITESKQMQNQLLESERKYRD
                     LVENMAEGLITIDEQKIVTLVNSKICHMLEFKPEEMVGKNIFDFFDSKNQKILKQELV
                     KRPEGTFSRYEITFTTKSGKQIPALISGAPNFDEKGRFRGSYANVLDFTERKQAEEAL
                     RQSEEKYRLITETTSDLISLHTFDLNASYTYVSPSIKRIAGYEPEEL"
BASE COUNT        10529 a         7708 c         6592 g        10597 t
ORIGIN      
        1 gaatcacaag ctttaaatta taccaggtga gcgccacact atttgttatg cttcctgttt
       61 tccagaaact gctttaaatt gaagtgattg ttttgtagat tttctcatag atgtttggtt
      121 tgttttagtt attaaataac atatcataac ttaatacatt acaatgtatt aaacaaccaa
      181 acatctttat ttttatgaat tattcaggct aattgagcta caagccaaac caatttccgc
      241 catgccgcaa gcgtgactaa attgatttcg gcggaaagaa agtttaagac tgagaaaaga
      301 aaggctaatt cttaagttct ttcttaatct ctatgattat ctattatccg aattaatcag
      361 gataaaatcg cctcaatttc ctctttttct aacacttctt tttctaaaag cgtatttgcc
      421 attttatcca gtaattccct tttctcaatc aacatctctt tggtttcacg gtagcattta
      481 tcaataactt ctttgacttc ttcgtcaatc aatctttcga gatgttctga gcgcctttca
      541 attccagctg attggttgag aaatcccggt gttgaacggt taactagaga ataattcggc
      601 agtttttcgc tcataccata cacaacgatc atatttttca ctaattgagc cacgcgttcc
      661 aaatcattcg atgcgccagt cgagacctca ttaaagacga tttcttcagc ggcacgcccg
      721 ccgagtaatc cctttatttt tccaataatt tcagatttag tcatcaggta acgatcttct
      781 agtggcattt gcaaagtata accgagcgca cctattcctc tcggcactat ggatactttt
      841 tgcacctgtt cggcgcctgg agtaaaatat cctacgatcg catgacccga ttcatgatag
      901 gcgacgattt tacgttcttt ttcgttgata attttatttt tcttttccaa accggcaaca
      961 agacgttcga tagcggcttc aaaatcagat tgggtaactt catcgcgatt gtttcgggcg
     1021 gctaaaagag cggcctcgtt acatacattg gcaatttcag caccggcaaa tccaggcgtt
     1081 tgggcggcta attgctttaa atcaacattc gagctgagtt ttagattacg agtatgcact
     1141 ttaaatatcg cttctcgacc tttcatatcg ggacggtcaa ccagcacctg ccgatcgaat
     1201 cgtcctggtc ttaggagagc ggggtcaagc acttccggtc tgttagtagc tccgataatt
     1261 attaccccga ctccgggatc gaaaccgtcc atttcaacca gtaactggtt gagcgtattt
     1321 tcacgttcgt cgtagccgcc tccgtaagtc agagcgttac cacggctttt cccgatagca
     1381 tctatttcat caatgaaaat aatgcaagga gcggcggctt tggcttgctt gaacaaatct
     1441 ctgacccgag ccgcgcctaa gcctacaaac atttccacaa aatctgaacc gctgaggtta
     1501 aaaaatggca ctccggcttc tcctgccacg gctctggcta gcaaagtctt gccagtcccg
     1561 ggcggtccaa ctagcaataa gcccttcggt aatttgcccc ctaatcttgt gtatttttta
     1621 ggattcttta aaaaatctac aacttctttt acttcttcaa cggcttcatc aacgccagcc
     1681 acgtcggaaa atttgacctg attctccggc ttttcggcat atattttagc tttattccgg
     1741 ccgaaatcga gcatcgggtt gctacttccc attcgtctga aaattacccc ccataaaccg
     1801 ataaaaaaga taataggaaa tacccagttc agaaaaaaat tacgaagcga attcgactcg
     1861 tacctacctt caaaattgac acccgaggct tgtaatttcg ccaacagttc cggatctgga
     1921 agttccggaa tgcggctgac gataaattgc cgggctacct gtttttcgat cgagggcact
     1981 ctcaatcgcc acggtgcggt cggggcatga gtaagcgctt gctcttcatt aatatcttcg
     2041 gatttgaatt cgcctacgat gcgatcctcg aaaattacga ttcgcgctac ttttccagaa
     2101 tccaaagcat ttaaaaagtc tctatagctc aagcgaacat tagccggtct atccggaaaa
     2161 aataacaact gggtaaaaat tatcatcagt gtgattaacc agaagtaaat cagtgaaaat
     2221 ttttcatttt tcttcataac tgtctcacct catcagtttt caattgacac ttcatttatg
     2281 aaaaatttaa atcatttcgg cttattttgc cattataatt tctctcggta actcaggatt
     2341 cataagatta aggagttatg tcagcctgaa aacatattta attttttcag taggtgttat
     2401 aaggcttgaa tcaaaccgca ttttcttgaa attactttga aaattattaa atatcaaatt
     2461 cttacgaata tgctgaaaat gccagtaatt ttaaactaaa catctttttt gactcgcgaa
     2521 aattcttgca tttccagaaa taactcacta taatttatca cttttaaaac gagaatctac
     2581 aacatggagg acaatttatg ttgaaaaaga agaacgccat tttgattttt tggctcttgg
     2641 cgagttttgc atttgccgag gaaaaaggcg gggtaatacc ttgtttagca agttttttta
     2701 ttggcccgcg tgtaggtttg gaaatgaacg aaggtaagca gattgagaca atcgaatgga
     2761 ttcattttgg cagtaatttt gtcccatatg tcggtctagt tgtaaaggct tatacaagtt
     2821 atgaaattgg gggaaaaact aatggagcag gaggattttt tgcaagttat tgtattggcc
     2881 ctcgagttgg ggctgagcta aattaccgaa aaataagaac tctcgaatgg ttaagattag
     2941 ttccgtgtat taacatttat ccattcatca aaataccact ggaagcctat cagggcaaaa
     3001 caatgactga aattgaacaa aaagaaggac ttcgcaagta aattcagatt ttttatgaat
     3061 tgaaagctgg aattaaagtc ggtttaattt cagctttctt tttaaatcca attcgatggg
     3121 taaaaagata aaaataaaat tggtgatttt ttccatctta ttattttgga atttgttgct
     3181 ttcccaagaa caccaaaaat ggacagacta ctgggaaata cctttaaatt taccagaaaa
     3241 acccggggaa atattttcta ccaacttatt ttcttcttcg gctatagcct ggattaaaat
     3301 ttaccaaaaa gtgattgcca gtcaggattt accatcgtgc gtcttccacc cttcttgttc
     3361 acgtttcgcc ttgacctcca ttcaggaatt cggcattgtc aagggcgctt tattagccag
     3421 tgatcgcttg atgcgctgta acccatttgc tatgcgctat tatccatttg acggcgaaaa
     3481 attagccgat ccacctcaaa aatatcgttt aaaatagacc agagccattg tgaaaaaatc
     3541 tattttattt cttctcattc tttcgatttc aacaactgcc aatgctcaga tagaatcttc
     3601 tctttttgaa ccggcacact taaaagaatt tggactttac ctgtttcagc aaaaagatta
     3661 cctgcgagcg gcgatggagc tcgaacgtta tcgctatttt gcaaaaagtg gcgatgattc
     3721 gattactttt gttattgggc tgtgccatca attccgccag cgttatgatt ttgccgccgc
     3781 aacttttcgg gaactggctg aggatgattc cagtaaattt aaagaatctg cgcgcttagc
     3841 tatgttatat aattatacaa aattagaaga ttggcctaaa atcctgggat taagctatca
     3901 aaatgatgcc gagttttatt tctattattt agccagcgtg aaaacagctc caactacaat
     3961 taccacttcc ttttttcaaa atgttaatga tgaaagtctg cgcaatcaac ttaaagcact
     4021 tgaacaggat cgtaataaaa ttcaccccaa atcacctttt ttagccggaa cattatcaac
     4081 tcttatcccc ggattaggaa aagtttacat caatcggtcc ggcgatgcac tcttttcgtt
     4141 cggaatgatc aatctctttg cgcttctaac ttataaagcc tttcaagccg aactgcctgt
     4201 aactggcgtt ttgtcaggtg gattgactct ctcgctttat ctcggaacaa tctacggcag
     4261 ttatgtcggg gctgaacttt ataatcgtaa cttagatgcc gattggcaaa gtaaaatgac
     4321 tgaactgaat ccggttgatc ggcaccctta ttggtccaca tggtaaaaag aataattctt
     4381 ttactacttg cgatcttctc ggtaattttt tctacggaaa atctcggcga ttactataat
     4441 gccaaacaac attatttcga agccattact gagtacaaac ggcaactatt ttttggccag
     4501 tacgaaagcc aagatgaaat tttactaaaa atggcaaagg tataccacgc cgttcggcag
     4561 gatttagtcg ccgaagaaat acttttacaa gccatctcca atgatgagac gtctgctttt
     4621 gactgggaat gttcactcga attagctcgt ctgcattggg ataattataa ctatttcgaa
     4681 atgcgcaatg tcttggattt tattagtata aattcagagc aaccaattca ggatacaatc
     4741 aattatatca aagcctggac ttacttttat gaagccaagt ggggcgaagg tctggctttg
     4801 ttggattcgg tccattttat tgactcaaaa tcattgcaga aggaaattgc cggtgttcat
     4861 aaaattcctc aaaaatcgcg cactctggcg ctggtaatgt caaatatcat ccccggcacc
     4921 gggcatttgt acgcgcacga ttaccaaaat gccctttact cttctctttt ggttggcgga
     4981 acgatggttt cgatcatttc gaatgtcatc gatcgagcct attttgttgc cttgattaaa
     5041 tatctattta tctattcccg ttataataat ggaacattgg ttaacctcgc caaaaaagta
     5101 gatagagata atattgatgc gattggtaat tatctaaaaa ctgtctcgca gaaatatcca
     5161 gcgcctctgg aattgctgga gtgtttgcga tgatataaaa gccttttaac cacaaaggat
     5221 ctaaagcacg attaataagg ttgaggctga gagaagttat tggtcattgg tcattggtca
     5281 ttagtcattg gtcattgggc attagtcatt agtaaaaagg ctgaggctaa gaaaaaaagc
     5341 tatactgtca ctctgagcct cttctcttct gctctttttc cccagccttg accttaacat
     5401 tggtttgtag taccgcactc cacttaagtc tccgataacg ccttaagtca aatgcattga
     5461 ctaacggccc accagctcca ttagtcttag agcatcataa tacatggtaa aattattaaa
     5521 cataatgtaa ttgagttctt gatcacatag ggcgagcact ttttccaatt cttcatcgct
     5581 aaaagtataa ttataatcta atctattccc gatgtgtagt ccgtgtaaac gataatagcg
     5641 gtaatcgcca taataactgg gttgatataa tggatcaacg caatgaatca ggtttaattc
     5701 ccgacatact ttttggatcg ttttcttgtc ccatttggga ttgcggactt ccagaatgaa
     5761 ttcgaaatct tcactgtctt ttctaatctt ctcgaaaaaa ttatacaggt tcagcaaata
     5821 tttctcatct ggagtaaaac ttggtggaca ttggaataga atcttagtgc aacccaattt
     5881 caaggcaaat tctcgagttc tctcccaggc ggtaaagacc tcatcggtat ctttgaacaa
     5941 tccgtaattc tctttagccc caattttttc tctcagtcgg cgataagtga acgaaccagc
     6001 cggatgagta atgatttgcc aggctttaat gacgaactcg aatttcggat tctgcttttc
     6061 ggccgccaga cgccattttt cggcggtgac aatttgaggc aattgataaa aggtgatgtt
     6121 aatttcgata caattgaatt cacggtaata ttgctctttt tctatcggaa agccgcaaca
     6181 accgattttg aattctttca tatctaaaat gtagtcaaat ccttactaaa aagtgacaaa
     6241 gaatttctaa gtttgtcgaa agccctttcc ctgcaagatc acgattttca aaatcctccc
     6301 cttagccgac aatgaaaata ttgatttttc aggttaaata ttagtaacta atttaacgaa
     6361 aattaataat taaacctttg tgccttcgtg tcttagtggt aagaaaactt agattacttc
     6421 cagaattttt tcttcgatat tttttttagg ttgggcgccg gtaatctgag ccattatttt
     6481 accatttttg aaaatcagca gagtcggaat actacggatg ccgaattttt gggcgatttg
     6541 aggagcgtgt tcgacattca ctttgccaaa tttcattttg ccgttatact tttgcgccag
     6601 ttcctcaatt accggcatca gtttcaaaca aggagcgcac caggttgccc aaaaatcaac
     6661 cagcaccggt aagttggatt gctcgacttc agccttgaaa tttgattgag tgaattctac
     6721 cataaaaccc cgctttttta ctacttgatt atctcaacgt ttcacgctat aatatattct
     6781 taactacttt taaactcaaa tctatttttt ccggaaattt tttatctggc gcgcacccat
     6841 tgccatattt ccggaaacgt agatcttttg ccatatatta aaatcgccac tctgaaaatt
     6901 ttcccagccg ccagcatcgc cagccaaaca cttacaacca atattcccaa cgaagtcaag
     6961 atttcccaaa gcggcggcat cattacaccc attcttaaaa tcataaaata gggtgtgatg
     7021 atcggaatga acgataaaat tatcgtcgtc aacgactgag gattggcaat tacgtaggaa
     7081 gccaaaaaaa tcggtacaac cgagatcaac gaaaaaatcg aaacggcttg ctgagcgtct
     7141 tgttcattgt cgaagagcga accgattgcc gcaaagatcg ccgcataaag caaataaccc
     7201 aaaacaaaat agaccagaaa taagatgaca tttattgaac tgaataactc gaggcttttg
     7261 aaattcgaaa tcattattcc gacgattaaa tagagcacca attgcgtcaa acctaacaat
     7321 cccaacccca aaattttccc ggacatcagc tctgtgggag acaccgagga aagcaatatc
     7381 tcgattaaac gattagtgcg ttcggcgagt acactacgca tcaatacttg ggaagacata
     7441 aaaatggtga agaacaacat tagcacatag attaagggaa taataaacga gagggtctcg
     7501 tcgcccaaag tctcttggcc gctggaagcg actttgatgg tctggaattc gacatttttc
     7561 atcagatcct ggattaaagc cgggtctaaa ccggctttaa ccaaccgata ttcggaaatt
     7621 atttcattga tcaccagatt aagttcactt tgatctttga agttgccgac attcaagcta
     7681 taataaaaca cgcggctgga atccagcaca acttctggaa taacgagata agcacttata
     7741 actgaagaat ttaaaagctc agccgcttct cgtttcattc tcacaggatc accatttttt
     7801 attacgatga cttgatattt cggagtttga tccgcaagag tatatttatc agccaacttt
     7861 tgttccaatt tctccgcaaa ttgaccagtt tcatctacta cggcaatcag gcgcacttca
     7921 tgatcgtctg agtttaacac gactgatgga atgaacatcg aagccaccat aatcagcgga
     7981 ataataatcg tcgagatgat aaatcctttg gaacgcagac gagttaggta ttcccagcgg
     8041 gtgattatca ttatattttt catgaatttt ccgcccccct cacaagctcg ataaagattt
     8101 gctccagaga tggctcctgc aattcaaaac gagttacctg cacacgttgc attactgaga
     8161 gtaataattc attgagatta ccggtaagac gacaggtaag tcggttgtca gtgatttgca
     8221 tatcaggcaa attaagatca gccaatgtat ccgcttggcc atcataatca atcaaaagca
     8281 ttggttgacc atattctttc ttaatctgcc gcaagtcgcc gtttagaatt ggtttacctt
     8341 tatttatcaa gcaaatccgg tcgcatagct tctcgacttg ttccatttgg tgagtcgaaa
     8401 atattacggt cgcaccgcgt ccctgtaatt cagcaataat ttccttcaat agcagctgat
     8461 ttaccggatc aagaccagca aaaggttcgt ccaggataag aagttccggt ttatgaatta
     8521 ccgaaacaat gaattggact ttctgttgat tgcctttgga aagttcttga atgcggcgat
     8581 ggctataagc ggagagatcg aaacgacgaa ggtattcgtc aacactttgg ttggcttctg
     8641 ctgatgataa acctttaaga cgggcaagat aaacgacagt ttccttcaat ttcatttttt
     8701 ggtacaatcc gcgctcttcc ggcaaataac caactttcaa taaatcagat tgctccaggt
     8761 gttttccatt ataaagaatt tctccggcat caggctgaat gatattcatt atcatccgaa
     8821 tagtggtagt tttcccggca ccatttggcc ctaataaacc gaaaatcgag ccagttggta
     8881 tattaagatc gagactatca accgccaccg ttttattgaa tactttccta acctgtttta
     8941 attgtaacat tcagcaatct tcgatttatt gaattcaggt gattatttaa cgatgaattt
     9001 gaaaaaccgc cgtttcccga ctttggtgat atattcgcct tgattttgaa aggaataatt
     9061 gatatcgtta attttttcgt tgttgatgcg cacaccgccc tgatgaatta agcgccgagc
     9121 ttcaccgtta gaagaaaccg ctccgcattc ggttagtaac ttgactaaag gttttggtaa
     9181 atccgatgag ggaattatta tttcctcgat gtcgtcaggg atttctttct tgctgaaaat
     9241 ttgttcgaaa ttttgttcag cctgacgggc ggcggactct gaatgataca ttttgataat
     9301 tgtgcgggct aattgctttt ttagcaccat tggattggtc tgcccgtcat ttaggcggcg
     9361 ctttacttcc tcaagccggg caagcgaata atccgtcacc agagtaaaat atttaagaat
     9421 cagattatcg ggaatggaca taattttgcc gtacatctcg tccggttcat cgtcaatgcc
     9481 gatataatta ccgagacttt tgctcattcg ttgaccacca tctatgcctt ccagcaccgg
     9541 cagagttaat ataacctgtg gttcctgacc gtattcgcgt tgaacgtcac gagcggtaag
     9601 cagattgaat ttctgctcgg tggcgccgag ttcgacatcg gctttgattg ccacggaatc
     9661 gtagccttgc atcaatggat agaagaattc gtgcaaacta atcggattgc cttctttata
     9721 acgtttggta aaatcgtctc tttcgagtat ccgggcaacg gtaaatttcg ccgccagatt
     9781 cataatttca tcgaatttca ttttggcgaa ccattcgccg ttgtaatgga tttcagtttt
     9841 acttctatcc agtattttaa aaaattgatt ctcgtaagtt tcggcatttt ttatcaaagt
     9901 ttcatattgt aatcttggtc gtgtttcgtt cttgccactc ggatcaccaa ccaagccagt
     9961 gtagtcgccg ataataagaa cgattgtatg tcccagttct tggaactgac gcaatttccg
    10021 gattccgact gtatgcccaa ggtgaatatc cggtgcggtt ggatcgaagc cctgcttgat
    10081 tctgagcggc tgattggttt taattgagtt ctcgagcttt ctgaccattt cctcttcgga
    10141 aattagttcc tcgactcctc gtctgatgat ttccagttgt tctttaaccg gtgggaacat
    10201 gcctattttc cttttcttaa acgttcaact tcgcggcgcg cttctttctc agctagcgcg
    10261 ttcctcttat cgtactgtct tttgcctttg gctaaaccta tttcgacttt aactttcccc
    10321 ttatccgtaa taaaaacgcg gagcggaatc agggttacgc ctttattctc gacttcgcgt
    10381 tgcaggcgcc gaatttcatt tcggtgcaat agcagacggc gatcgcgttc cggattatga
    10441 ttgtcgtatc cgccaaaatc gtagggagca atatgacatt tcaataaata taattcccga
    10501 tcaatgattt tggcgtaact gtctttgaga ttgatgtgcc cggctctgac cgacttgact
    10561 tcggtacctt tgagttcgat gccagcttca attttatcca ggataaaata atcgtggaag
    10621 gctttcttat tagttgttat aattttgaca tcatcttttg gcatacggaa aatttaattc
    10681 ttttgcctca acttccaatg taaaacttgg agaactcagt cggaggttat aaatttcgcc
    10741 gttatttgaa gcccgggtgg tggaactggt agacgcgcta ggttcaggac ctagtctcca
    10801 ttaggaggtg cgagttcaag tctcgccccg ggcacaaaga ttgaataatg cgaaattagg
    10861 cttaattact tatatcggta accaacgctg ttcggtttgg aattatttcg gtaacttgac
    10921 aacgtctgtc attcggtcgg ctgaaaaaag tgaactttca gcaatttttg ttccggaggc
    10981 agagagataa tactggttat ttcgattgga gcaataggcg ttattttcag accaagcgcc
    11041 aaaaactgag cttcgatagc tttgtaattt ataatcgagg aatcgtaacg gaaagtgcaa
    11101 aatccgacgt tttaatcgaa aatgaacgat tcgatgccct ccgtcatttt cattaaatcc
    11161 tgcatgtttt tacccgactg ctcatccggt aaaccttcga cccgaactgt cattaattta
    11221 ctgctggatg aatattcctg tccaacgaaa agcactccaa tgcaaaacag gatcatcaac
    11281 gaaatcatca aaatgcccaa attgcgtcgc ctcatacaaa tgcttccttt tagctactac
    11341 cccaaaaaaa gcgatataat ttaaggactt actaactaaa atgaaagtaa tataatggca
    11401 attttaaaaa tatttctaat caaaggacaa ggaatttgca ggttacttta tcaacaatat
    11461 ttttatcgtt ttggagtaat taccagattg catcctaacc agatacgttc cgcttgaaag
    11521 tagcgagcca tcaaaaacaa ccgaatacat tcccgcctgc tttgaacctt ttactagtgt
    11581 tgctacttct ttgccgagaa gattataaac ttttaatgtc acatgcccgg ctttcggtaa
    11641 ttcataaggg atagttgtag tcggattaaa gtctgttatt cactgcgtta taagtatcat
    11701 tcggttcagt gaatagcgta gttacttcgt agtacggaga tggcgccgaa ttgacatcgc
    11761 catattggtt aaatatttta cccttaattt tgtacttcgt accgtatttc tcctgccata
    11821 ctacaccgat ataattatcg acaacatagc cttgattatg agtcgtaaag gcaatcgaag
    11881 gacacttgcc gccggcagtg ccatctaatg gctgtccatt attgccaaat gtccaggtac
    11941 tgccaccatc accggaatgc tcgagccaca cgtgaccggc gctggtgtaa acctggtgaa
    12001 gccaacgggg atttccacca gctgaagtct ggattaattt ccgctgactg gtgttaaatg
    12061 ccgcggcatc attggaacga tggataccct tccagtttgc ctgcaggttt ttatcgctat
    12121 ctattatatt ttcattgaat taactctagc tgacagaggt cataatatat ttgcgatgga
    12181 taaataaaag gcataatact aataattaca tacaagctat ctccatttgt cgttgtaaga
    12241 gtatcaatta actcatagta attccaaata gagtcctgaa tcgtgaaata ctttccatta
    12301 cttctatttt tattgaagtt tacgtatccc ttaccatatc cttgaggatc tttaaatttt
    12361 gcccaaattg ataatctata acgattgcaa cctagcggtg cagctatttt tgtttctaaa
    12421 tacataggta ctattcggtc tcctccataa agaacaacag accagttgcc tccgaacggt
    12481 ggaacatcat tggaaaatcc aacatcgaga aaattactat gtaaagtcca accgtcaatt
    12541 gatttattgc cattaatttc aaatgaagag ttggtaatta agtttaatcc ttcttcggta
    12601 agctctgtcg gacattcttt ctcacaacca gatataagaa taacaagcat agaatatatt
    12661 ataattaccc gttgtgtgga attaagcgag ggcatgttta atatggttta agggtttatt
    12721 attgatagaa ttcaggaact gtagcaaagt gaaggcagtc actttcgaca aaatcctggt
    12781 agccaagcca acaaagattt tggtataatt tatacgaatc acgaattgat ctaccaaatt
    12841 agagaacaag gttccccggt tgtcttcgat aattcttcag attggagcgc atggttggct
    12901 ttggagagat caaaactggt tatgacccca ttaagactgc aaaccccatg gaatttatac
    12961 ccgaaataat gggagttttg agccgcgcaa tagccgtatt ctggtgctgt ttcaatagat
    13021 tctttgataa ttggtttaat acgatcgaag gttaatttaa gattgtgcat tagtttatca
    13081 ccttgattat tattgaatta caatataata atttaaggat tttcaagcta atgcacatct
    13141 tattttttag gtttttattc cacacgacgg gtatataata ttcttttgct gtcattgaaa
    13201 aatatggtta atgtattgtc attgatttta taagatgttg cattttgaag tgcacttagg
    13261 tatttccaat aatttgatct gcaccacgct tccgttgaaa taattaatcc gatggaaaga
    13321 gaatcgttgc ctccaatcca ataaggggca tggtaattgt taccgcataa agtatcactt
    13381 ttaccgtata tagtactatc accaaaaagc aaaaaataat cttcggtagg ggctaatgtt
    13441 attatttctc cggtgacact ttgaaaagac tgcacttccc accatatatt cttgagattt
    13501 gcgggaatgt tctgattcgg cgaagtcgag tcagaacatg ctcctaacgc aagtagtaag
    13561 gtcataagta tatagtttat attgtgcata ttttctcctt tcaaaaaatg ttatatgata
    13621 ctatcaaaat tggttcatca cggaattaaa cttgcgctga cttaccggaa tattgtttgt
    13681 ttggaaatct gaatctcgag acaaatccat tcctttccta caatccttcc cttctgagtt
    13741 agaaaaaaaa tcatacggtt ttccgctctt ttgggttcat cgaagactct tcttgcttct
    13801 ggtataatct atatgatagg agggtaaaat caagagtttt tttgtaagat tatttctcct
    13861 gccaaactac accgatataa ttatcgacaa catagccttg attatgagtc gtaaagacaa
    13921 tcgaaggcat caacgaatat ttgaaaaatg tttcgccaga aggttaagca tacagaacag
    13981 ttcacctttt tacctccagg ctatggtcag catttattat ttagatcttc atcgtaagag
    14041 aagcaattta tttgtcttag aaaaaatacg attatctgat tcagctataa ttctacaaaa
    14101 gtagatccca cttgaaacat tatttccaaa cttgtccttc gcgtcccata agattttgta
    14161 atatttcgct tcctggtttt cattaacaag cgtaataacc tctctaccaa gcaaatcata
    14221 tatctttata gttacgtaac tgcgctctgg cataccgtaa attatattcg ttatgggatt
    14281 gaatggattg ggatagtttt gcctcaatga atattctttt ggtatctggc tcattaagta
    14341 ttcaattccg gttgcccttg gtgtcgcact tactggttgt gagtaagagc tttcattgaa
    14401 atcagtgctt atggcagtga acatatagaa gtaggtagtg ccattctgga cagttaggtc
    14461 ggtgtaacta ctgtctgaga tcatcgaggt gttcaccagc gtggtatccg agaaggtggt
    14521 atcggtgagg ctctggtagc gatagagatt gtagcccatc aggtctatca tctcgggttt
    14581 gtgccaactc aggtcaaccc tcccattacc tgcttgggca atgaagcctt cggaagcggc
    14641 accggcagca tccactacaa aggcaaacct ggtatccttg ggaatctcca taccctcgca
    14701 atcctgggcg gtggtcaccc gcagcctatt tatcccatcg ccggtcatta ggttgaaggt
    14761 ataagtccct tgccaatgag tactgtcaat ccaatcacct tctactatat gttgggtcca
    14821 ggggtcacat actccaaaag taacataggg ctgtacacta atatccattg gtttgctgaa
    14881 gatgaggttg aatgtatcca cctcacagcc tataggtgag gggggattta attgtacctg
    14941 atagagaaaa ccgggagcag tcacaacagg agtagtttca aacggctcat agaaaacctt
    15001 ccccaaacta aagtcatcat aataatccca aatccaggcg tcaatggagt cggtactcga
    15061 ggtaaaccaa tagttattca aagaattaac gtttttacta tcgtccattt taatctcata
    15121 ctcatcgttc ccaaaaatat tattagaatc cattcttcct tcaaaattat atccaaatac
    15181 tacaccatta caactgttga acattatatt attattaata aaatttacat tttgagataa
    15241 ataaacacca ccagcatcgg aaggaatgta actagagtaa ttgttgtata tattattttt
    15301 aaaaatatag agatttcttc ctgaaccata aataccactc cctccactat aacttcctct
    15361 atttccacga attatatttt gagtgattat ggcagaagat gtatatacag ctattccacc
    15421 acctgactca gacatattat cttctattat attgtaagct ataattggaa ttccattgca
    15481 attgtaaagg taaatacccc cgcctctgga gttactatta ttccggatta agttatgctc
    15541 tactcgcgaa gtagaagaag atactaaggt aatgccacca ctagattcga cacccgtgcc
    15601 ctgcacacca taattatcgt gtataaagtt atactctata taaggggagg catcacttat
    15661 actaatacca ctgccaaatt caatctccac ataattaaca attgaaccgg ataaataatt
    15721 accatttgca tcaaatattg cgtctctact tttatcagtg aatctaatac cgtaccaatc
    15781 tcccggtgca gggttttgcg cattggaagt aaagactatc atactatcct cggttcctat
    15841 cgccacaaga cagccgtcaa tcctcaaata tttctccgga caaaattgaa tccttgtccc
    15901 gggttcaata gtcaaggtta ccccttcatc aacaagcaca ttgccagtta caatatataa
    15961 atagtcatta gttaaagtcc tgtcttctct tattatccca cttatctcaa tacctctttg
    16021 tgttttaata aaaaatgttc cattccatga atatccgcca tcacaggtta tttcataatc
    16081 aaaatataca tcggtattat tgggagtgct gggcttcaca tagaacaaga acggattgct
    16141 tccattatta acatgagcat aggcgctgac gccaccgcca aaatttgcta aggaatccag
    16201 tatagttaca aaacaggtat cttcgtagga atgcggtcgt aatatagctt ctacgttata
    16261 cgcttgcgcc caggtgtttt gaatatcaaa aaccatttcc accgtttctc ctgcgtcagc
    16321 tattctatca cggtcatcgc caggaagtgt atcagttatc gtatatgaat aaagtgaaag
    16381 ctggggttct ccgatttcaa ttagtgctct atgcgcgtta agtctattgt atccattaat
    16441 cggatctgtg gtgtaaacca gctgcccatg tatcatttcg tttgaccagt caggatactt
    16501 agagcgtatt agcgcggcag tcccggcaac gtatggagaa gccatagatg tgccattcca
    16561 ggatgcataa tcgtagcgat gaggatgaaa tagtggaact gtactcaaaa tgctaactcc
    16621 aggtgcatac aaatcggcat taataccaaa gttagaaaat tctgctttag tctcatacca
    16681 aaccaccaca acattttcac cctcagggac caatttataa actacatccg atgcaccaac
    16741 gccgataaca aaactccaag ctgcgggata gaatggtgca ctcgttccat tattaccagc
    16801 tgatgctact ataacagcag tagtataagc attttccagt gcatctctca atagataaga
    16861 ttctgcatag ccaccaaggc ttaagttaat tatctttgct ccattgtttg ccgcataggt
    16921 aacgccattg gccacatctg aataagagcc agtgccgtta ctctgcatta ccttaacagg
    16981 caaaatctta cagttccacg ctacccctgc tattcctatg ctgttgtcag ttgtagcggc
    17041 agcaataccc gctacatgag acccgtgccc attatcatcc atcgcatcat tgtcattatt
    17101 tacaaagtca tatccagtac ttactacttt atccaccaga tcgggatgtc cccaatccac
    17161 gccagtgtct ataattccta taattacgga tgtatcccct ttttccacct cccaggcttc
    17221 cggagcatca atatccgcat cttccagatg atattgaccg gtattatgta atccccactg
    17281 ctgatcaaaa tagggatcgt ttggttcaac gcagatttgg gcgatataat tcggttcagc
    17341 atattcgatg ttattgtcat tggcgtattc ttttgagatt tcaactggat caatctcagc
    17401 cttatatttc aatttataaa ttttatcgag gttctgaact tcatgaactt ttccctggtg
    17461 atctttaaag agcggcttcg tcaaatgcgg tttggcatct ttgaacacct tttccatttc
    17521 ctcaacttgg tatttttgat ttagctgatc aacagcggta attccggtct gctgaacacc
    17581 tttggcaagc gatcggcgta tctgggtttc atctttgaat tttaccagta tctcaccctt
    17641 tacatattca ggattatttg ggtcttgttt aggtggtaca atcccacctg agaaggctaa
    17701 ttcagccaaa aaaataacaa tccagagaca actaaaaatt cttttgctca tagttacctc
    17761 ctgagttatt gagcaacttt gagtcaagtc cttacaaagc ctttcaactt tagtgtaaac
    17821 ttatacaaag gacttgactc aagttgtttt gtggtatttt gtccaaaacg atccataact
    17881 ctcctaaaga gttattttta aaagtaaata tataatattt aaatcgaaat gtcaatggaa
    17941 aagcttcatt tcactgtctg atctagggac atggtatcca cttttggtga taatgttcag
    18001 ataatgatca gtgatgatag gtgggaagat gccgatggtt tcaggattaa tatttggcgg
    18061 attatattca tttagtattt cgattggggt tttcatatac ttatagcgaa acttacgctg
    18121 gtagttgaaa taaacgcaat aggtaaatgc tttgctaagc aattcgccga tgttgtggta
    18181 atcttccata tcatagaact cgtcttcaat tagccggtga gcggcttcga cgtcgctgtt
    18241 ataggtttta cggccaactg ggatttgaat cgattcggaa tttaatgctc caacgatttt
    18301 ctcatagagg gtctgtttat ttctctggtt ccaggggcca ataaactctg agccgttatc
    18361 actctgccag actgtttgat ttaagtcaac accacaacta agcaaatgac agcccagcag
    18421 gtaggtaaag atggctacat tggtagttgt tttctcgtaa gcatatccta tataaattag
    18481 cccggtgcga acatctcggg ctgtaatctg gtatttggga taattatgcc ataggtaggg
    18541 ataatagcgc ggaatatcgc ttaattcttt taggtcaatt tgaatcttct caaaaagcct
    18601 catcttggat ttaacatttc ttaaatccct cttgacctgg ctcttgcgct tacgctgacg
    18661 gatcaaaccg gcattcttta tgactcggtg aatggtagcc ggactggagc gaataccatg
    18721 ctcggcttta atcctgatag gtcctaaata cggttggcga ttacggatag ctagaatctg
    18781 ctgctcctct tgaacagtga ttttaaaggg actatgatga gggcggcgac tgtgatctac
    18841 cagtcccaat gtcccctcgc tttgataacg ctttagccat ttgcgaactg tttttctggt
    18901 ggtaccaaat tgattagcgg ctttgcttac tccattatcc agcgcgtaat gaaccatctt
    18961 aaaacgaata tcgtaactat ttttcatttc cttgtaggct tcataatagt taatttcaat
    19021 catcagggct ttctccttgt tttaaccagg tattttacaa ctgctaaaat accagagaaa
    19081 gccctttatt ataagtcgtt caaaggcttg tttcgctacg ctcaacaagc ctttgaattt
    19141 caaaaaactg ctctgcatta atcctattta tttctaaaag gtagatacta tgtcttgata
    19201 ctatacatat ttgatacaat atcaattatt ttaatctgct gaaatctcta taaaaatcat
    19261 ttattcagga tccgcaattt ttccgcttct actctgcctt tttcttcatt gacgaaacag
    19321 agtaaaattg ccccaaccag aaataatatt ccaatcgaac caattccaat ccgtgaagcc
    19381 aaatcagccg aaaatccggc gcggcgagcc aatagaccca cagttgcgat tagaaacggc
    19441 ccgaaaatca ccgagaactt accaatcata ttgaaaaaac catagaattc ggcgctctgc
    19501 tctttgggta tcaaccgtaa ataaaacgaa cgactaagag cctgaatacc accctgaaca
    19561 catccgatta caattgccag aatataaaat tctttcacat ctttcattaa aacgctatag
    19621 gccgttactc ccagatagac ggcaattgtc gcaaaaatta ctttcttggg attccatttt
    19681 tcagccaatt taccaaacac cagcgccgcc ggaaaaccaa caaactgaac aatcaacaaa
    19741 gccacaatta atgaactcga cggaaatcca attgacaatc cataatctac cgccattcgg
    19801 ataatcgtat caacgccgtc aatatataac cagtaggcta ctaaaaaaat catcacgact
    19861 ttgagatggc gcacttgctg gaaggtttgg ctcagttgct taaatccctg acggatgtac
    19921 gtagaacgtc tattcggtct cggctcccgg cgtttttctt tgacataaag aattagcgga
    19981 atagtgaaaa ttccccacca gagccctacc gataaaaaac tcacccgaat cggcagattt
    20041 tcttgggctg gagtaagata atcggtggta atcgtaatcc gcccacagtg attggaaagt
    20101 ccctccactc cctcaatccg ctgatcgcga aatgattgat aagtaatcgg atttctaatg
    20161 ataattacag cggaatcctc cggactcaat ttttggttca gattaccaat caaatattca
    20221 ttcttacctg gctttttagc gacgatctcg ccagatttaa attgactgaa actaatcgca
    20281 gacactatca agtcctgatc aatgtcaggt ggaacaggca cactgatcca gactgaatca
    20341 tcagaatgat aatcaactat cgtgatgggt aatttaattt cactggtgac aactgcttta
    20401 ccgattgaat gtttcttggg aatcgaaaaa tccttgcctg gttcgatctg gataaccgtg
    20461 gcattttggc tgagagcttg atagactttt gcctgttgct cgaccggaaa cccaaaagtc
    20521 gccggactaa gataccacaa aacattaatg agaaataata atccacctcc cagataaccc
    20581 agcgcatagc ctttggcaga cacataatca attttatgag acggggcaac ctccggcaat
    20641 aaactatcgt aaaaggtatt tgcaccggca aaacccagcg acccagccac atagaaaata
    20701 accgcccaca accattgccc atagtcaacc ataaaaagac aggtcgtcgt cagaacaccc
    20761 aaataagcga aaaatatcaa aaagcgtttc ttcgatgaac tctgatcggc aattgcgccc
    20821 agaattggcg atattagagc tacaattagc gaaccaatcg agttagcgta tcctaacatt
    20881 gcggtactga cattcacatc cgtaccggca ctccaataac ttttgaaaaa caccgggaag
    20941 aacccagcca ttaccgtcgt ggcaaaagcc gaattagccc aatcatacat tgcccaggca
    21001 aaaacttgct tgtctttaat taataacact gttactcatt attatatttt aatttattgc
    21061 tattcattgc gattcaattg ctaattttgt gtcgtaattt tctcaacctt aaccttttta
    21121 ctaatgacca atgactaatg accattctcc gctttcgtcc ctttcagaat aaaaatccta
    21181 tatcaatata cagctggcta ccagcctctt cattgaaagc catttcagca tagacaatga
    21241 aattttcatt ccaaccgata tgaactccgc cgcctttccc agtatgcaaa ttggtaaaac
    21301 caccttttaa atcattttca tgccagaccc gcccgaaatc gaaaaaagcg tttgccgcta
    21361 ggtaaaaatc ctgaccggcg aactgaaatt cgtaaaaacg gtagcgcaat tccatattca
    21421 taataaattt ggctgagcca acatagcggt ttttataagc gccgcgaatc gaacgataac
    21481 caccaacgcc ttcatcaatg cggaatgaac cacctaatgg ataatacaac agaagaggcg
    21541 cctcgccaaa tatattctca actataattc gatttgcata taccagattc ttacgaagtg
    21601 taaaataacg gcgatcagtc agcgttaatc ttaaaaaatc attatcacta ccaattagac
    21661 cggtgtacca ttcgcccaca atatccgtcc agacaccgcg attcggcgcc ggttcattat
    21721 cccgcgaatc gtaaaccaac ccgaatttaa ggtaatcgtt aaacgactta tcagtatcgc
    21781 gttttttaag gattccagcg ttaaggtctt cttcaaattt ggtggcaatt ccgccgttgt
    21841 catctgcgcc acaacctagc tggtaaaagc ccagtccacc caatactgaa atgagcggcc
    21901 gcccatcctc ccgatatttc aaagctttca aaatattagt ataaaaaatg atttgctcgg
    21961 aattaaaagt atagtaatgc ttgccgtgca aggaatcaag actattgcca tcctcatcaa
    22021 cttcgatata actgggatcg aatttcgaat cgttgcccaa tccataatag ggctggaatt
    22081 tttctttttt ataacgggtt cggacgctta ttctgaaacc attacccaaa atatagggtg
    22141 aatcaaaaaa gatggtgtgg tcctgttttc caccggtcgt gaagaaaatt tggttatcaa
    22201 ttttaaaata gtaaggcgaa tagccacctt tggcataatt gaaaatatca aagatcattc
    22261 cgtaaccaaa tccctcatcg gcgttgtaat tgatagccgg aacgccaccc caaccccaac
    22321 cggtgcgttc cttagccatt gtcccttcga tggaaattag cgatattagt gctactatta
    22381 ataataagtc tctcttcatt ttaacctcaa aatataaata ggagaagccg taacgacttc
    22441 tcctatttat catttaatcc tggcataatt ttacagcttt acctgaacgc cgaaagctac
    22501 gagataatta gtctgattgt acttttcaat tccgctggca ttctcagcct caatatagaa
    22561 ggcatccagt gcaccgacgt tgagctttac tttcggtgaa atttgatact caacgccacc
    22621 gccaattgta aacgcatcga gattgaagtc catatcggac tgcgattccg gttttgcacc
    22681 catcatacta tattgtccac cgagactgac tttcaatttt tcggttaaag catattcagc
    22741 acccaacccg aattccgtcg tattgtcgaa gtttttctca tcgccatccc agtcaacggc
    22801 gccgttattg taataattaa aatccatcat tagtttaatc tttcccatat tataagannn
    22861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgt tattgtaata attaaaatcc
    22981 atcattagtt taatctttcc catattataa gatacgccaa cggctagctg agaaggtata
    23041 tcggctccat atttagcgct atcagggaag agaccggtat cgtctttctt cgtatcattt
    23101 tccagttcca tcttagtgat ggtctcataa cgaataccga tattcaagtt ttcattcggg
    23161 ctgatattaa ttccaaaaat tcccgcaacg cctgaaccac tctgagcggc gtcaacttcc
    23221 ttatcagcag tttgagcatc taaattggta ccgtatgaag tcatggtgcc agcaactgtt
    23281 gtataagcat tttgaacttg agccacagta taaccagttg gattgatacc tgcggctatt
    23341 aatccacttt ctaattgcgc aacctgttct tctgttaaat atcctgctgc cttagcatct
    23401 tgtaaagtaa tatttggcgc tgcagcaact aaagttgata aactatttgc cgcgccagtc
    23461 gcagctgttg caaccccaaa aaagtaggtt gaaggttttt cccaattacc ggatcgtaaa
    23521 acctgaatat tcttcaaata tccggtgtag ctattacttg ccataacgta gcgcgctccc
    23581 ccaaataccg aaaatatctc attaatttta tatgaaacac cgccttgcac accgtagtag
    23641 atcgaagaac cttcaaaact tacatccagt ttataatcag taacgcccac gcctgctaag
    23701 gcagttttca gtgccgaaac cggaacttca aaggaaggca aacccttctc aaatttggca
    23761 ctgccgccgc ccccgatcgg cataaagcct ccggaaagta caagtttatc cattttataa
    23821 gcaacgtaag ccgagggaaa aaccggcgca aatactttgc cgacgaactc atgattgttc
    23881 aacgtcgggg tgtcattagt caccgtcttg gtttgccaga tcgtctgatt gctgacatcc
    23941 agaaacaaac cgtcctcgaa taatttagtc aatccagccg gattgtagta aaccgcatcc
    24001 acatcggtgg aagcgttacg gttcaaagtc cgcacccaag ccgcgctttg gttagtgttc
    24061 gtaaccaagc caccagctaa aacagttccg ctcaccagca gaactacaca taacaaaaca
    24121 cgcctgattt tcatgttttt ctcctttatt tttactcgtt attagagtta tgatgaagaa
    24181 ttttcgtttt tattttttcc ccttcggcta ccctacagtc ctgctttacc ggtatttaaa
    24241 ataaccactc aatctataat taaccactaa cctcggcgta aatatatcga aaagaaaagg
    24301 aaaacaaaat taattttttt gaaccggata caggcaatat ttcaatcgta atttttgaaa
    24361 tcggctggct ttcgcctccc agtcagctaa catcggattc aagtcagcgc caatattgac
    24421 ccaggttcga aaatcagcat tgccgaaata agcatcggcc tctgtggttt ttttccattt
    24481 aaaatgtttt gggtaaagtt tggatatcac tgaaattaaa gtcgcgctaa atttataagc
    24541 atcaaacttg ttaaggtcac taacttttaa gtttattccc aaacattctg tatccagata
    24601 taaaggcatt tcagacgcat aaagttcgcg acgtggaata aacttgacct ggcttatttc
    24661 cactcccgca agatcatatt tcgccagagc gtttactaat tcattagccg acatccaagg
    24721 cgctccgacg acgtgaaaag ggtgaggcgt tccagtcccc acactaatat tggtaaattg
    24781 gaaaaaatac aatccggcat agagaacagc agtctcttca tcttgcaccg attcatccgc
    24841 aaatgaccat ttcagtccgg tttgttgcca gggcatatcg cgataataat ttgccatttt
    24901 aattacggta agttgagatt tatccccgtc attaagccag ttttcctcat tcattagcaa
    24961 agctaattcg cccaaagtca taccgtgtcg agcaggtaaa gaaaagtcag ccgcaaactg
    25021 actctgaatt gacattggac cacctatttc aactccgcgt aaaggattgg gtctatcaag
    25081 cacgataata ggaatttttt gcttagcgct gaactttaaa gcctcaatca aaactctgag
    25141 cgcctctgat tcccgcaatc cgaaatcctg caaatcgagc acaatcatct cggcgccatt
    25201 taacatattt gcactaagtt ggtctctctc actatttaaa atataatatt tggtaattcc
    25261 ataaagctga ctctgactta atcggaatcc tgagccggtc agtaacgagc ggtcatccaa
    25321 aacctgtacg cacgaccgac agcgcgttcc caatcttata ttgacaatgt caaaaatata
    25381 ctgatttccc tgagcaaaac tattgcgatc ggcaatgacc ataaatttat aattatcgag
    25441 agcgtgaaaa ttatcatcaa ttaacaaatc caagccgatt ttaaagtcct gcgtttcttc
    25501 atcaaccggc aaagtaatta ccgtagaatg ccgtaatttg gcagtttgac cgtttatttc
    25561 gctaaagact aaaagtaata atattaattc gccgaaaaat gaccgacgtt tatcccaaat
    25621 tattttcata agcctgctaa ttctcgatct ttttctttcc aaaatccggg tcaattttta
    25681 ccagactggc gactaaaaaa ctgggaatcg tacaaaacat tatatagaca aagaaagttc
    25741 tatatcccat caaatcctgc aatttgccgc tgatcattcc cggcagcatc attcccagcg
    25801 ccataaaagc cgttccaatc gcataatgcg acgtcttata ttcaccttcc gccaaataca
    25861 acataaagag catatagccg gtaaaaccga aaccgtaacc gaattgttca atggcaacac
    25921 aaattgtaac tatcataaaa ttatccggct gaacatatga taagtaaaca tagactaaat
    25981 cgggaatatt aatagccagc gccatccata acacccatct tttcagcccg ttgcgagcgg
    26041 caactattcc accgagaatg cctccgattg tcagacaaat tattccgacg gttccataca
    26101 caaatccaac ctgcgtcgtt gtcaatgcta atccgccagc ctcttggcta tccagtagaa
    26161 aaggcgaagc catttttacc aattgcgatt ctgccagacg ataaaataac aggaaagata
    26221 aagccgaaac aatgcctttt ttctggaaaa aactggaaaa aaccttaagc cagctggatt
    26281 tgagttcctg gctttttacc ctgaccgtct ccaatccggt cggcaacatt agtttgtgat
    26341 acaaacaaat gagcaggaaa aatacgccta aaattccgaa cgataccgcc cagctgaaag
    26401 gaatatcacc ggcggtagct tcaaattgcg acactaccgg ttctcgcaga cggtgatcaa
    26461 ccttcaaagt caccttgacc ggcttattcc aattctcagc cgtaaattcc aggcgaccag
    26521 ctgatgtcaa ccagatgtcc ttactacctt tcttgcggcc gaaatttact accacagttt
    26581 cctctttttt ggggggggct gacagacaga taaaaacatt agcggaatca ccatcgggct
    26641 cggctggatt ataaatcggc acctttactt ccgccggaat gatgactata ttaggttgct
    26701 cagatgcctt gatcgctggc aaattatttt catccagcac ttgcataatt tcattcggcg
    26761 gaacagccat agccgtaata ctaacaggct ttagcccggt tttactcata atataaccag
    26821 ttagcatcac gattaagcct tgcccgacga tcattgccat ccggtaaaac gtactgcgaa
    26881 tcccgacgaa ccaggtctgc tggtgcgcat ccaagccgat catataaaat ccatcagcgg
    26941 cgatatcgtg agtcgccgag gctaaagcaa ttatccataa aacgattaac gtcaaagcga
    27001 accagagcgg taatcggaca actagcgcta ccccgataaa acaacatccg agtataaatt
    27061 gcgttattaa tacccaattg cgtttggtag aaattacatc tacaaatgga ctccagattg
    27121 gtttgattac ccagggcaaa tacaaaacac tggtccaaaa cgccaaagcc gcattagaaa
    27181 ctcccatcgt tttgtacata attaccgaaa ccgtcatcgc tatgacgtag gggatgcctt
    27241 cggcaaaata aaggcttggt acccaaagcc agggtgaacg atttttcaca gtcgccggtt
    27301 cacttgccat tacttttctc ctgagtaatt caatatgctt ggtggagttt aacacccgga
    27361 taataatgtt ctaatatttc caaggttgaa tagcctttca gagccatccc gagagcccca
    27421 atctggcagt agccaactcc gtgtccccac ccagctcctt tcagaacgaa agctaccggg
    27481 acattttcag atccttcctc cacatcaact acaaaagccg aactgtaaag gaaaagttca
    27541 tgaaatgctt cacgaatggc atattgatca ttaatcacga tttgcttgtc aacattggct
    27601 ttatcccgat aattaatagc aaggctggtc aatcgtccgg acttgccccg ttttaatggt
    27661 tcgatgctct tgatcgcggc cgcgtccaga ttcaatttca gattgaataa cgccgtgatt
    27721 tctttctgcg tcatccgaaa atgccagcga aaataatgac cttctttatc cacattgcca
    27781 agatactttt tcaactcttt ttccggtacg atggatgggc tacagaaagc caacggcggg
    27841 ttttcgatcc aattgcggac tttcgcctct gtatcgagtg gcaaagccgg atgttggcaa
    27901 tcaggcggaa tatcaagcac tgaatccaaa tacggcacag ctgacccgtt ccacacattt
    27961 tcaaaagatt ctgtaactcc gccgcagttc ttgtgatagc gggtatcaca gattttttca
    28021 ccgtatatta agacctgacc ggtggtttca agcgcacctt taatcgaatg ctccgaaaga
    28081 aacgtcgtcc cctgataacg ctgacagcaa tcgtcattgc aaacgtccat tccaagattg
    28141 cggtgtttca tttccacgtt cgccagcaac caactgcggg cgatgattgt ctgcgatttg
    28201 ataacggata atggacaagc cgctcccatc tcggaagttg ctacacacat taaatattgc
    28261 tcaagcggca atacatttat cataattaga ttgtcattca tccgacttat ttcgattgag
    28321 ccgggcagat aaacatcaat atatttttcc caatgaaaac cacgtccggc aaccacattc
    28381 ttgactttaa ttccggcttt gttcgcaatc cgaatttcac cgcctctcgg aatgattttc
    28441 caactcggtt gagaattttg tagcactcca ttaatctgaa ttccgatttg gtttgcagct
    28501 aaaataaatt caatctgttc gccgcctttc aaaatctgcc gcgggccgct ctccggaatt
    28561 aattcataaa ctggagattg tggaatcacg agcgcaatcc tttgacattt atcaattggc
    28621 aggaaaattc ctacccgcag gattggctct tcggtcggaa tcacattcgg ttcaagcata
    28681 actttctctc cttccaggtt aaataagcgt caatagccgc acccagcgct ggcgcttctc
    28741 gcagagggga aatttgaatt cgcgccaggt ttaaatgctt ttgcggacaa taatgttctt
    28801 tggctacctc gtcgaggacc gaagaattga taattaattg agtcaattca ttgacaaacg
    28861 gcaaccagag cacctcatca ccttgggctt cgcacaataa ttcacccaat cgctgtccga
    28921 tgaccagatt gtcaaataac gcctttttat aattattttc agtttggggt cgtggacgat
    28981 cgggattgac aaagtcaaat attccctgcc aaccggcata tatcgaggta attcgctcgt
    29041 agaaaagaag cgccagaaaa tgagcaactt tttgcatcgt aatagccgcc gctttctcgc
    29101 cttccaaagc ccgtcggcga atttggggcg gaaagatacc gttccgggtt aattcctctg
    29161 gcgactttcc gattagctcg ccataaatcg attgtattcc gctcgaagaa acgtattttt
    29221 ccacggaata gccatccgga cacttcattt cccaggtttt aacaaaccaa cctttggttt
    29281 cgtcaagcgg aatgactttc ccgtcgagtt tcagagcatc cgccgcaccg gttcccccac
    29341 caaggtaata agaattgcga acattccgaa acgaaccttc accggcatat tcttcgccga
    29401 ttccacaata ataagcgtca ctgccgatct gattgatcgg ttgaactagc tctattccag
    29461 ctaaggttat ttcattttcg agtcgaaccg cataattggt aatgcgcggg ccatttttca
    29521 ggacggcgat gccgcggccg tctctggttt tgatacctgg catgccaatt ccaataagca
    29581 cccgattggt gccactccgt ttggtaattt gttcaataca ccaggaagcg gctttgatat
    29641 aacaactcga ttgaacgagt tctgcgttcg tcggcacgat ttttcctgct tcccactcgg
    29701 caatttgccg atttaagtca accggctgga aattatggat atagccgggg atttcattat
    29761 aggactttgt cgcgtggaaa ttgccaagtg caaaagcggt tccgtgattt gcggttttca
    29821 ctgaccagcc gctgacttta gttgcaccgc cgtcaattcc aattaacaaa aattcatcat
    29881 ctgctatgtc tttcttacaa ttagccaatt ttaaagtgac ttttccaaat tacgattagt
    29941 gatttcattt atttttttcc gtcggtgaac ggctttcaaa cgcatatagt ctttggcttc
    30001 gtcgttacga tcaatagtat tttggtcaat gctatgatcg gttccaccgg tatgccgcac
    30061 aacttcataa atcggttgcc agactcgtcc gatgcgatat tgctcactga tttttaacac
    30121 caggtcataa tcttcgccat aattgcaagc aaacggttcg tcgttcatcc cgaaataacc
    30181 catttcccgg ataattttta tcggaatcgc acgcggagct ccggcaccat tgatacgcaa
    30241 gagattatta cgtccgttgg catccgtcca ctcttcgtgt ttgacaatcg gaatttcttc
    30301 cattcggacg agttcaccac ttggtttctt ttcccagact tcgtacgaac cgataaccat
    30361 tccgattctt tcatcagaat tgaagacagc tacaattttc tcaatcgcat ctgattttaa
    30421 acgatcatca gaatcaagct ggacatagta gcgaccgcgg gcaattttta aaccaagatt
    30481 caacgatagt ccaatattgt taatatccgc tacgatcagt ctaaccggcg gttgctgagc
    30541 atcataacgg tctccacccg ccatataacg ccggacttca gtaaccgtcg gatcaagagg
    30601 acctccattt acaacgacga taatttcaag gtctttgatg ctctggctga agaccgaatt
    30661 cagcgcagta ccgataaatt ccggccgatg ataaaccgga attattaccg aggccaggca
    30721 atcgtacttt tcgtttagat ccggccgcag gtgataacct tgatcaggcg cgagataagc
    30781 tccgattctc ttcaaatgat tagttaaaac tacttcggct tccagctggg cttctttgtt
    30841 caccaacaaa taatcaaaga cattatgttt aactccgccg gataccactc gataaagcga
    30901 gccgtctatt ttgttggcaa tgtgaaccaa tttatatttt tccgataact ttagacgaat
    30961 gtcatataag gcgttgtatt tcaatgactg atcaaaaccg ccaactgctt ccaaagccgc
    31021 cttactgaac aggaacacct tgccataatc ctgattatca cgtaaacgac cggaatgatg
    31081 tttcagaagt cggatttcct gaatttttcc attattaact aaatcatagt cgccgtataa
    31141 cattcccaga tcaggatatt tttctgccag caagaaggat acatcaagca aggaatgttt
    31201 caaaatgact tcggcaaccg aattatcaat gaacaaaaga tatttgccct tagctgtttc
    31261 catcaccctg ttcaatgttc ggctaagatt acctgcctcc cgaattatct ccaatggaag
    31321 tgagcaacaa tcaaattcag gaaccggctg atccgcctga cataaaacca gaacttcaag
    31381 cggcaaacag gattgattaa aaaccgaagt caaagttcga gcaaactggt ctgccatctc
    31441 gggaatgtaa tgtaaaatga cactaactgc gctaatggta ttagttgatt tcataagcaa
    31501 taattgaaac gttttggaat tccaaaaaat cttgccatct atctggtaaa actactgata
    31561 aactaatttt cacgaacata aaactaataa attccctttc aaataactaa acattttagt
    31621 gacggctggc aatttccaca attaaaaacg cccaattacc agaggttact tgacttttcc
    31681 cgatagaatc ggacaaccaa ttatttcaat atcggtctaa tcgcttggta ttatttagag
    31741 atttgaaaat gaacaaaata gacggcaact cttcgaaata tgaaaaccac aaacatttaa
    31801 aattttgaaa ataatgagga ctcaaatgaa caaaacgatc aaaattagtc tccttctcct
    31861 tttaattgtc ggtttattgt tttctgctta tcggctggga agcaaaaaag acaacactac
    31921 gattctctcg gtctcaacta cggttcagga cttcaattca gtgttagcta atagcttgga
    31981 acaattagaa aaaaccgcta atgaggctga gttcggcaag atcatcctaa atgtggacga
    32041 taccgctatt atcgtaacgg ttaaaatgaa agatttgcgc aaactgactc gtaccgaaat
    32101 tccatttgac caatttatcc gcacctattt gaagttttcc taagatttcc tcacctgcag
    32161 taagcaagcg cagaaaccca aattttctgc gctttttttt acctaaaaat ataaggtcaa
    32221 attaacagca taatcggtaa ttttaaattg gtaaatataa cggaatattt aggtgcaata
    32281 actaattaaa ttcaaaaaga aaatgtctct caagcaaaaa aagttatacc aaatagctta
    32341 atgactttta gcatattcag gttacttaat cagaatatcc tgaatcgctt gatcgagttg
    32401 attcttgggt aaagcaccaa ccagcatctg cggtttgccg ttttttggga taaataacaa
    32461 tgacggaata ctccgaatac tgaaatattc agccagttcg ccctcttcat cggtattgac
    32521 tttatagata ttaaccttcc cggcgtattt tttactcagc tcctcgagaa ccggcgccac
    32581 cattttacaa ggataacacc aatcggcata aaagtcaatc acactgggta attcaccttt
    32641 atatttccag gtactttcat tttcaaaatc gaaaatttcc tttaaaaacc tggctttagt
    32701 caatttttca ctcattgctt ctccattttt tactttaata tctgattcta aagccccaaa
    32761 tcggttacaa ttttttaata aaatagttct cattaaacaa attgaaaatt ttattgcttt
    32821 actttactat ataaaggtag tatatttttt tatgttattt ggctcaatta tattttaatt
    32881 aaaataacag attggtttta tgctaaagac tgataagaaa cagctggata aattacttga
    32941 gcttcagggc gaagtcaaag gggtggtatt tcagactgac ggtaaatatg tcttgcaaaa
    33001 agaaggcgag gaaggattaa aaaaattaga aaaaagggtc aaagaattag gttatcctat
    33061 tgactatagg aaaaaaggtg aagccttaga tctgtctccc atcggcttaa gagtcatttc
    33121 ccttctgatt attcaagaca cttttaactg gcaagattcg gaaataagaa atatggggta
    33181 tgcggcgccg ggattttcat ttgttgtaaa actatttatg cactttttta tttcattaaa
    33241 aaaatcagct aacgaatacc catcatattg gacattgcat tataccatcg gcaaccttga
    33301 ggtgataagc cttgatgaaa aatccaaaga tgtgacaatt caccttaatg attttaaggt
    33361 tcatccaatt atgtgcctat atcttgaggg atactttgaa aggatttatg aattcgcggt
    33421 tggaaaaggg aaaggtaact gcaccgagac taaatgtatg tttgaaggcg atccttatca
    33481 tgaatatgtt tttaaaatga aaaggcaata aaatatggta ttaactgcag agactcgaga
    33541 attagagaaa tatatagaag acatctggca atttttacct ataccggttt gctatataaa
    33601 tgcggttcat ctaattatag ccgtcggtgc taattttgaa aaattctcgg ggtatgattc
    33661 agaagaactt gtcgggatgg agctcgaaac cttatttgcc gatcaacaag aaattaagaa
    33721 aatagaagaa gaaatattaa actcaggctt tgtttctaat agagtaataa ctttcattac
    33781 caaagaaaaa aatgaagttc cggttcttct ctccgccacc gccagaaaag atgaagaaga
    33841 caatattttc ggctatttta tgtcaatgct ggatatcacc aagcgcaggc aatacgaaca
    33901 ggctttaaaa gaaagtgaac gacgattttc cgcttttatg gaaacactcc cccatacggc
    33961 ttttatcaaa accggcgatc acaaatatct ttatatcaac aatgcaatga agaaacgctt
    34021 tggaattgaa gagtgggttg gtaaaactac ttttgatctt ttcccaaagg aaatagctga
    34081 aaagttggtt aaggctgata atatttcact tgtcaaaggt tatcacatag aagagagtac
    34141 cgtccccgat aaaagtggca atgaatgtat ctgggaaacc tatacttttc gaatcgagag
    34201 ggaaaatcaa gagccattaa tcggcggttt ttcaatggat attacggaga agaaggaatc
    34261 tgaaaaaaaa ttacgggaaa acgaagaatt tctaaatagt attattgaca gtatccaaga
    34321 cggcattagc attctaaaac cggatttgac gatcaatttt accaatgatg tgatgaaaaa
    34381 atggtatgcc gagaacctgc cacttgaagg gaaaaaatgc tacgaatgtt atcacaatgc
    34441 taacgctccc tgcgatcctt gtccaactct tcgctgtatc caatccggca aaaccgagtg
    34501 gaatattgtt ccaggtctcc ccggctctcc tatcaagtgg attgaattat tcagctatcc
    34561 tataaaagat ccggatacag gcaaagtaac cggtgtggct gaatttgtgc gtgatattac
    34621 tagtaaaagg caagcagaag aagctctacg tcagtccgaa aacaaatatc gcttgttatt
    34681 tcagacttct ccagacttaa ttgctcaggt tgataaaaag ggtagattta ttaccgcgaa
    34741 tcctgcgatg gcagatagtt tcggcatctt atcagaagaa ctgattggta aaacattttt
    34801 tgaagtaatg cctccggata ttgctcagca tcgtctcgag atggtacgaa aggctttaaa
    34861 agaagacaga atccaaaatt ttgaagatga aagagatgga aaatattatt ataatttatt
    34921 cgtcccagtc aaaatttctg aacaggaaga agccaccgtc caggtaatcg cgagagacat
    34981 taccgagagc aaacaaatgc agaaccagtt attagaatcg gagcgtaaat atagagatct
    35041 ggtcgagaat atggctgagg gtttaattac tattgacgag caaaaaatcg taaccttagt
    35101 gaacagcaag atttgtcata tgttagaatt taaacctgag gaaatggtcg gaaaaaacat
    35161 tttcgatttc tttgattcca agaatcaaaa gattctaaaa caagaattgg ttaaaaggcc
    35221 agagggcaca ttttctcgat atgaaatcac ctttactaca aaatctggga aacaaatacc
    35281 agcgcttatt tcgggagctc ctaattttga tgaaaaaggt agatttcgtg gctcttacgc
    35341 caatgtatta gactttaccg aacgcaaaca ggccgaagaa gccctccggc agtcagaaga
    35401 aaaataccgc ttgattacag aaactacgag tgatcttatt tccttacata ctttcgattt
    35461 gaatgcttca tatacctatg taagtccttc tattaaaagg attgccggtt acgaaccgga
    35521 agaact
//
LOCUS       DAOSMP010000021        35463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_1234_length_35463_cov_23.818572, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000021 DAOSMP010000000
VERSION     DAOSMP010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 35463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35463
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1234_length_35463_cov_23.818572"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PKX09_05130"
     CDS             complement(<1..211)
                     /locus_tag="PKX09_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17239.1"
                     /translation="MNDFAGKRFNLFGASRVGVSLAYHLNRLGFKPEFVWNRSPERLA
                     QAKQAVPFQNFSTDLTAACRDKPDWI"
     gene            complement(393..>1252)
                     /gene="hflX"
                     /locus_tag="PKX09_05135"
     CDS             complement(393..>1252)
                     /gene="hflX"
                     /locus_tag="PKX09_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOU17240.1"
                     /translation="RWTHLERQIGGIGVRAGSGETQIETDRRLIKTRIAKLKSELVAI
                     DRQRTVQSQRRANCFRIALAGYTNAGKSTILNLFTGADVLVEDKLFATLDTTVRQLNL
                     DGYPNVLLSDTIGFIRKLPHNLIASFRSTLREIIDADLIIKVADISSPHCLQQLVSVE
                     EVLNELGAGNRPSLIVFNKIDRLDENVFKIAQKTYPEAVFISALNQLRIDDLIQALER
                     AIAREGRHLRIQTPFEEVSDCALIRNHTTVQQEIYEDKVVIFEVFCYKNVWNWLARQL
                     SSATTVLEM"
     assembly_gap    1253..1352
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1353..1584)
                     /locus_tag="PKX09_05140"
     CDS             complement(<1353..1584)
                     /locus_tag="PKX09_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17241.1"
                     /translation="MSKLPDQSKIFPTGAVLPSEKVLLVGIINSNQPRHITEEHLTEL
                     ELLMNTLGYEMADKQFVNQRAVDPAYFIGRGKA"
     gene            complement(1581..3665)
                     /locus_tag="PKX09_05145"
     CDS             complement(1581..3665)
                     /locus_tag="PKX09_05145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HOU17242.1"
                     /translation="MSFLNPGFLWLLPLAGIPLLIHLLGRQRFQVVEFSSLRFLKSLQ
                     ADVLRRLKIRQIILLIIRTLLILCLILIFARPYRSSQTPGIYMAKGANLYLIVDNSAS
                     MSLKCDGRTQLSRGIEAILSSAKNIDFPVNLNLILATETAGISNRTTVYSLSDLEREL
                     VHTPETNFARQLLPAIALAVEDIIKNQDLNGVIWLVSDFQANNWNRKSPMEQKLLAEI
                     IANKIRLVLFPVTGEADNSALGAIHFNEQIYAKDKPVTLQSTTINWRRQPQENLVSLF
                     IEEERVAQALINLPPAKSEQATFEFIPLNSGILSGYLQIEDDDLAIDNRRFFILNIPA
                     TLRILVVGSQATDGDYIMKALQANPDQMIEARFTTSEYLGNEILSSYDGIIFSEVVSL
                     SSVSQKNLSEYLQNGGGILIFLARNSTPEQFNALWAERFDLPRWHNTREAGDDGYLGV
                     GKYDSNHPIFRDLWPENVNPTATPRFFKAPGLICGSKHKILMSFDDNTPLMVETHYEA
                     GRAVMMATVPKADWSDLPFSGFFPVILQRMTQYIAGGATQPLNYTNGDTVKIARIPGE
                     NSIPQVQTPSGRRFNPSVVSNYFSFDNTAEIGIYSVIREGGMIEKLAVNLPLSETAAE
                     FFSVADYKNLIKNAPANITVVSETSTDEISQLTLTREFSMLLLFCALFLAIAETFIGR
                     LNRIEMVNPPAE"
     gene            complement(3711..4982)
                     /gene="hisS"
                     /locus_tag="PKX09_05150"
     CDS             complement(3711..4982)
                     /gene="hisS"
                     /locus_tag="PKX09_05150"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOU17243.1"
                     /translation="MLYQRVKGTRDILPSEVHQWQFLENFLREYFKRNNYKEIRTPAF
                     EVTELFTRSIGGDTDVVSKEMYTFLDKGGTSITLKPELTAPVMRAYIQNNLEQIAPLT
                     KVYYIDSLFRQERPQKGRLRQFHQFGFEIIGSPHPEADAETIQTVYELYRELGLTELN
                     IRLNSIGDRLTRGQYLIVLRKAIEPYHDELCGACQERLQKNVLRLFDCKVEICRQIMA
                     EHAPSILDYLSNDDRLHFKKVCHLLDVSNVPYTIDQALVRGLDYYTRTTFEITSPLLG
                     AQDALCGGGRYDHLIADLGGKPTPGVGVASGIERLLLVLEQVGSFPEPTDNLVYVITM
                     SENSRTAGFRLVTELRRKKVNAQMDFLRRSIKAQLRDADREQANWVVIIGEDEISRNI
                     VIFKDMKNGDQRELPLNTAADQIVALLQDKT"
     gene            complement(5017..5352)
                     /locus_tag="PKX09_05155"
     CDS             complement(5017..5352)
                     /locus_tag="PKX09_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17244.1"
                     /translation="MPFCPRCLCEYVEGTSKCIDCNAELVARLPEEKMTDVKWFCLGV
                     LSDIVMAEMVKESLENNGIETILKIDPLHTTFSLQSTGATGSYAKLFVPCGKKKTALD
                     ILKSMSTTG"
     gene            complement(5362..5823)
                     /locus_tag="PKX09_05160"
     CDS             complement(5362..5823)
                     /locus_tag="PKX09_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOU17245.1"
                     /translation="MELDKILTPRLIVYPLSAETKEEVISILVERLYDEGLISNPEAA
                     CQAVIEREKLMTTGIGKGVALPHGKYRETMEVLVAAGVSPEGIDFDSIDAQPVHIFIL
                     LLTPERFPSKHLKLLSKLSRMLNNANCRAEILAAKSAKEIADIFYKYDSQV"
     gene            complement(6175..6408)
                     /locus_tag="PKX09_05165"
     CDS             complement(6175..6408)
                     /locus_tag="PKX09_05165"
                     /inference="COORDINATES: protein motif:HMM:NF015927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HOU17246.1"
                     /translation="MDPFSWSYTFTMYLRAIGWALVAALGFSLGIGLAIKVFNLLSKD
                     INEWEEIKKGNISVALIIVSLILMVGILVYKVI"
     gene            6815..7633
                     /locus_tag="PKX09_05170"
     CDS             6815..7633
                     /locus_tag="PKX09_05170"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOU17247.1"
                     /translation="MKLKAEPEKISEAVEFLQKRLKNQSAKIAIVLGSGLGYFAEQLE
                     IVDRIDQQEIPHYPTSTVVGHAGKFIICNISGIPLIAIQGRTHLYEGRTLAEVTFYVE
                     ILARLGIRYLILTNAAGGIDPKLKPGSIVLLTDYISFTQIPLTPDGQSQTASPFCAEM
                     IAMVREIALQNKIELHEGIYCWTLGPSYETPAEIQAARKLGATVVGMSTIPEAVTAQR
                     LGLKVLGISLVTNLAAGISESPITHEEVQVTANRSKEVYSILMEKIIIELAKND"
     gene            7699..9867
                     /locus_tag="PKX09_05175"
     CDS             7699..9867
                     /locus_tag="PKX09_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOU17248.1"
                     /translation="MGRITKQTETELLSWPKALLYSFGVYLILGIIAILVGLYFSRDL
                     PSIEQLKQVNPELVTRVYSSDGVILQELYTQHRIYVPIEKIPPAMVQAVVSIEDYRFY
                     KHWGVSLRDFARAMVIDIITLSKKEGASTITQQLARMLYESIGFEKTFVRKIKELLTA
                     VQIERMYSKSEIIEMYLNSNWMGHGVYGIQAAAQRFFDKNSEQLTPDECALLAGIIKN
                     SVRFSPIRNPINAYQRRNLVLHRMHKYGYLSDEEYALYKNTPLQVFKPEIPPERAPYF
                     VEYIRQWLQREDEKLGIDIYRDGLSIYTTIDSKIQAAADSALTRHLQYQQNLLNSRLL
                     SNPSELRDFIKDTTISLAQVRDMIRGNIPMSPSMQASLVVQGALVAIDPATGHILAMI
                     GGRDFRESEFNRATQAKRQPGSAFKPIVFAAAIDNGFPVTTQLLNQPVVLNMPDGSRW
                     APKNYDLSTGGPTTLREGLAKSLNLISVRVIQELISPTSVVELAKRMHLTTNILPVDA
                     LALGACEVIPIELTSAFGIFENHGVWVEPIAITRIDDRYGNTIAQYKPRQELVLSEET
                     AYLVTSLLESAINVGTGAGARSVYGFRLPAAGKTGTTNNFNDAWFIGYTPYMVAGVWV
                     GVDNPAISLGNRQSGAAAALPIWANFMRESHNTKGWKAKNFERPDGIVEVQICSETKL
                     LPSTYCPIETEVFAKPTVPTEHCQIHNEVGKPRPKDNVVF"
     gene            complement(9926..11608)
                     /locus_tag="PKX09_05180"
     CDS             complement(9926..11608)
                     /locus_tag="PKX09_05180"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU17249.1"
                     /translation="MKIYLIQPPIEDFYTTPIRNIPQGLLALVANLPKYEINLLDLRS
                     RVRREIVPPPELASVRQFYRPDDASPFGLYKRYYRFGLSEMEIRQTLPRDGDYYLISS
                     LFITYAETVFELIRLIREIVLKSKIIVGGAAATIQPREFLEQGADFVIKGEGELALPR
                     LLNELEKDQPSLAPIPNLVWRDKTGVIQENPVNFIEDLNSLPSADYQTPGTPTYYYQK
                     KPHAILITSRGCPYRCSFCCIHQTMGSRYRLRSVENVLNEMAEKIAQGFRSFDFEDDH
                     LGGERDWFLALLKGIAERFTGLNLSLQAMNGITATNLDDEILTAMWRAGFRSLNLALV
                     TPSEAHQRQVGRPFGTEQYLKVVECANKLGFFITTYLIIGLPGDRAEELLESILFLAP
                     LPVLIGPALFYLVPGTASFQQFSRLGLIPSSVLCYRSSFYPIDSDECSRVAAMTLFRI
                     CRILNFMREVIDNEKTPHCPEIESDRIFLEKDLNGRESRYSLGLGLLKLLFEREILYG
                     TGRKEGNFYPIIREICDQKMIERFLSSTWQISGLHRPTIFLKDEFVRRFLLD"
     gene            complement(11806..12969)
                     /locus_tag="PKX09_05185"
     CDS             complement(11806..12969)
                     /locus_tag="PKX09_05185"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOU17250.1"
                     /translation="MKTVVMKFGGSSVADTTRLRQVAEIVVRRQQADNRVVVVVSAMG
                     KTTDALIAQAREVSANPQQRELDMLVTAGERISMALLSMAIQELGGDAISFTGSQSGI
                     ITEPTHQGARILEVRPFRLIEALEKGYIVIVAGYQGVSTTKEITTLGRGGSDTTAVAL
                     AAALGADSCEIYSDVDGVYTGDPHICPQAQLLREIPYEVMQTMALAGAKVLNADAVEF
                     AKKAGITILARKTGDSSGRETKVHNTAMGSGATAIVLAEKVSRINGNGNLPDLLMEIN
                     KAGGRVMALYQRATFDLLVDRTGIPGGDAGRVAQLAKAAGFSANEVAVVTVVGSGLGA
                     RPSLFEKVEVTLSEIDIPILAVFANDLALSIVIEPTYATGAVRALHQALIVGK"
     gene            complement(13133..14599)
                     /gene="glgA"
                     /locus_tag="PKX09_05190"
     CDS             complement(13133..14599)
                     /gene="glgA"
                     /locus_tag="PKX09_05190"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOU17251.1"
                     /translation="MNIIFIAAEASPFAKVGGLGDVIGALPPVLANRGLSVKVIIPGY
                     RSTWSFKTEPLTQLSNLTVTLGNRVVESQVRRWQNPAQIRHEVYFIDNPEYFGQRNVY
                     CAENGEPYPDNPERFVFFQKAALKLIAETRWSAEIIHCHDNHTALIPVYLKNNLAKVT
                     PFNSLKSVLTLHNIAYQGVCEMRLKEICDLPEELFKPFGALEWYNSLNPLKGGIIFAD
                     AVTTVSPTYAREIMTDEELGAGMSVVLANRPTPVVGILNGIDTAEWNPATDVHLFAPY
                     SISNLAGKKINKAKLIETLRLNPALKSRPLLGMVSRLVEQKGIDLLIDSLEKILSLGV
                     GFIILGSGEQHYQFELERLANQFRDQMAVKFDYDNILSHRIIAGSDLFLMPSRFEPCG
                     ITQMYALNYGAVPVVHHTGGLVDTVIPWDGKDGNGFAFCEYRSEALLKTISEALQVFE
                     KPDEWQKIIINGMSADYSWDKAATKYIELYENLVNNKS"
     gene            complement(14596..16344)
                     /locus_tag="PKX09_05195"
     CDS             complement(14596..16344)
                     /locus_tag="PKX09_05195"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOU17252.1"
                     /translation="MKGYFSLVLHSHLPYVINHGEWPHGMDWLYEAAAETYIPLLREF
                     NSLIADGISPNITIGITPILAEQLAAERFKDGFINYLEIKIKAARDNREEFATVGNLH
                     LEKLAGMWENYYQGLIDEFNSLPDRNIIQAFKKLQDGNHIEIITCAATHGYLPLLGND
                     EAIDAQVRIGKETYRRHFGCDPRGMWLPECAYRPSYQWSSPIKEYPKTFIRRGIEEFL
                     FKYDIQYFIVDKHLILGGKNRGVYIDRYESLKQLWQQFEKNWTPPPEEKSRSIYQPYL
                     VSSTGTGRVAVAYGRHEESALQVWSGEWGYPGDGRYLEFHKKHFPGGHRYWRVTSAKA
                     DLADKQEYVPENVEEALENQSDHFVEVLRNYILKYYAESGKIGVVTAPFDTELFGHWW
                     FEGPRWLGKVLRKINANPDIQAISLGNHLAKNRPTQVVTMPEGSWGQGGFHYIWLNQW
                     TEWTWKHIYAAEDKMVELAEKYRETGDVNLRRILNDLARELLLLESSDWQFLISTWSA
                     RDYAENRVGVHRDQFHNIVRILNDYENNGRLSETHQKYLERIERVDDIFPELDFTYWS
                     KLPPETGRVDSNNSSK"
     gene            complement(17049..17121)
                     /locus_tag="PKX09_05200"
     tRNA            complement(17049..17121)
                     /locus_tag="PKX09_05200"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17086..17088),aa:Phe,seq:gaa)
     gene            17510..17980
                     /locus_tag="PKX09_05205"
     CDS             17510..17980
                     /locus_tag="PKX09_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17253.1"
                     /translation="MKIFDNKGMLIIPNPMRGKFTEKPKLLVVNQLFCPNVHNLINQR
                     VTFNGYPGILIKVRQDEAIGLIALSPFYGEYSRFSLDIDLIDGKLLKLICPTCDVEMP
                     IIAQCECGGNLAAFFLTRECNFNDCVGICTRVNCHNSRIVHSGELITGSMLESL"
     gene            complement(18008..18724)
                     /locus_tag="PKX09_05210"
     CDS             complement(18008..18724)
                     /locus_tag="PKX09_05210"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOU17254.1"
                     /translation="MHTRHLFLLIGQSNMAGRGEMDRVLPINDDRIQMFRNDRWQKAV
                     EPLHDDRADLAGVGLGMSFASSILKRNLVQNVGLLPCAVGSTAIEDWLPGTPHYQRAV
                     AVVKTALATDTYENMLGAILWHQGEDDAKDKILAEKYRDRIVSLVWSFRRDLNMPSLP
                     FISGELGDFINAATGYPYGPDISAVLKELESILPDYACVSAEGLASKDDGLHFNAPSL
                     REFGKRYALAYQRLISYRLN"
     gene            complement(18752..19381)
                     /locus_tag="PKX09_05215"
     CDS             complement(18752..19381)
                     /locus_tag="PKX09_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HOU17255.1"
                     /translation="MARNEELYLAILKGDRAKVQAILQNAINLNQDVNELLEETMIPA
                     MREVGERFARNEIYVPEMLISARAMQAGLTLIEPILAKSNHKARGKVAIGTVKGDLHD
                     IGKNLVSIMLKGAGYEVLDLGVNCSVEKYAEAVNAGINILLCSALLTTTMMYMKEIVD
                     YFKSNNAVKIVVGGAPVTQSFADMIGAHAYGQNANDAVRIVANLTGAVV"
     gene            complement(19371..20312)
                     /locus_tag="PKX09_05220"
     CDS             complement(19371..20312)
                     /locus_tag="PKX09_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOU17256.1"
                     /translation="MIPIRLVTGFLGSGKTTLLKNLIRQNQSRRLVFLVNEFSPTDID
                     GSLIANLTPDYLSIPGGSIFCRCLAGKFIEYLREIPQRFTQEGNEIDELVIEASGVAD
                     PRVIFKMLSEAGLDKVYNIREVLNIVDPASLGKLFHTLTSIRAQIEAADIILINKVDI
                     ASPEEIASTESLIRQVNPSAYVYRTTFSQLPKDLPENPQRLQVPEGEFAPCRDPRYAT
                     IILNSGDMNYEQLRQAIIPVADDVYRLKGYLFDSHQYQYVDYSPAGWQIEPASSIAQE
                     PQIAIIVNGSNQAKVIEHFKLCGLIGNNVYFGAKNGT"
     gene            complement(20309..21706)
                     /locus_tag="PKX09_05225"
     CDS             complement(20309..21706)
                     /locus_tag="PKX09_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008102703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOU17257.1"
                     /translation="MENNSIQGLQLVRQAFNRQKTTRVPWVPFVGCHGGKLIGQTAEN
                     YLKSGKLIAEGLRKANELYRPDGLPVIFDLQLEAEVLGCELLWADDAPPSVITHPLDS
                     LNGKNLSDLPEFSLAKGRYPEIAQAMDIIRQDFAKDLAIYGLICGPFTLALHLMGNEI
                     FLEMYDHPEKVEELLAYCKEIAFQVSDFYLKNGADIIAVVDPMTSQISPQHFRQFVTP
                     VLNPIFDHIRAKGGLSSLFVCGDVDRNLVCMCETNCDNVSVDEQISLPKLRELAQKYN
                     KSFGGNLKLTVALLMGSADDCRLEAIRNIDEGGQVGFILAPGCDIPYNVPPQNLQAVA
                     EMVYDEYQREVARRTLTAREFEADWIVLPDYANREEVILDIITLDSATCAPCKYMVEA
                     ALKATADLAIKTTVREHKITTSEGIGMMKKLGVRSLPTICIDGEVKFSSIIPDQQTLK
                     AAILDRWKFKTGQDK"
     gene            21809..22732
                     /locus_tag="PKX09_05230"
     CDS             21809..22732
                     /locus_tag="PKX09_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOU17258.1"
                     /translation="MKNIVLSQDKFIPSQSGANITFHRLAWDSSSQPESIIAGSDLRY
                     YSLASENTALHSHEFAEFFIVISGKVRHIVNGETQDLQAGMLTFIRPNDVHSFEKLND
                     SVCEMVNVAFKLELLLDLSTYLGNDFFLRRYTGPVLPPTFTLSLVETEELALRLIRLI
                     TLQKTDLDQARLKLKMILAEIFTRFFLEWQTPDPSRPQWFDKLCSEMKRKENLIGGLK
                     TMLKLAPCTQQHLCKCFRQYLNTSPTEFINEQRIKLAAKQIIETDEKILAIALEYGFK
                     SLSRFYKIFHKYYGVSPARLRQISRQKVIPL"
     gene            complement(22918..23748)
                     /locus_tag="PKX09_05235"
     CDS             complement(22918..23748)
                     /locus_tag="PKX09_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17259.1"
                     /translation="MIEQILNWMKHVEIGEGDLTTESIAPYPLHSSGRLVMSSDTLFS
                     GRAIMEKLLESAGEQLQVAWLCNEGDEIPAGATIFEFNGNGAEILKIWRLLEWLASSL
                     SGLATATREVVKFLGDHNKKLVAGNSVSPIFEEFEKIAFTTGGGEMPFSGISQRIYLT
                     QNHFIYAGKSPDEVITTLVDELGVARKKIKIEVEVNDPIQFAEINKLDCDMIHLVDFN
                     DEQLRTVFEKLNPRHKTVVHLATLADFRPEYDDYFFKYVAVEDLHRTRHYSPARLIFS
                     "
     gene            complement(23753..24331)
                     /locus_tag="PKX09_05240"
     CDS             complement(23753..24331)
                     /locus_tag="PKX09_05240"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOU17260.1"
                     /translation="MKKSIILSLILLTVPALILAQPEVKGKAEPCQQVLKGSHEMHCQ
                     GQCKTPLADLLKLTDQQKVDHKKIMVKYQRLNIPLQADLKLARLDLKEAIDNLDQKKI
                     DESVKKINDIKAKLFKNKIDQRVEFLKTLTDEQRKILKEQPCGMHKVKKIKRLEGRFG
                     WDDMGKLGSEIDELLGLVMDIDVGDMEIEPED"
     gene            complement(24341..24847)
                     /locus_tag="PKX09_05245"
     CDS             complement(24341..24847)
                     /locus_tag="PKX09_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17261.1"
                     /translation="MKNNWLLSMSLILLGFAGQLAAQPHSFDESETSMAERREKMETL
                     RIYKMTEFLELTPEQSEKFFPRLKQYEKNVRKMQHQQMQLVREIDQITQNTDTTVTEA
                     DVKKFLRAMAQIEKDIIAEKEKFISGLSSILTPSQQLKYMIFDNRFRRHLMKTLNPPT
                     DKIEKQQK"
     gene            complement(24867..25469)
                     /locus_tag="PKX09_05250"
     CDS             complement(24867..25469)
                     /locus_tag="PKX09_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOU17262.1"
                     /translation="MKICKQIKDYLPLFFTGDLNESAKRLVENHLKDCPACRKFSYEI
                     QHLINDLQPDPVNIEPNYGAELVVKIQNRLHNERRPKKLKYYLVPVLGSILIFIIIGF
                     NLMRNQSISSQWLSNDNNIDLYMDLTHSGYFSEMPEDVFDFVNFNENNLITSELRQKI
                     SSVVLSTNSNLLIDDYIESTAFLTDQDFESVLEEIEKSIL"
     gene            complement(25456..25992)
                     /locus_tag="PKX09_05255"
     CDS             complement(25456..25992)
                     /locus_tag="PKX09_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU17263.1"
                     /translation="MAKQNVNHPVYDEQAFNNLVRENHQRIYGLFRRMVGSQAEADDL
                     TQETFIKVYKNLPKFRQESSIQTWIYRIAINQGLNYLRRMKIRQTLGLELADSAAEPD
                     EFQSNQANRNLLRRVIAKLPPRQQMVIILRSFQELSFKEIAAVLNITENAAKVNFSHA
                     VKNLREIFLKMGVTYENL"
     gene            complement(26168..26437)
                     /locus_tag="PKX09_05260"
     CDS             complement(26168..26437)
                     /locus_tag="PKX09_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOU17264.1"
                     /translation="MESSKLYVGNLKYSVTDEQLAQLFAQFGEVKSANVIKGKGFGFV
                     EMGSIEEANKAHDALDGKEFEGRTIRIADAHPPRTEKKETHEKQE"
     gene            26958..28772
                     /locus_tag="PKX09_05265"
     CDS             26958..28772
                     /locus_tag="PKX09_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOU17265.1"
                     /translation="MFKNHPKGLVVAFFTNMGERFGFYTMMAILVLFLQAKYGMNEST
                     AGNIYSWFYFSIYALALLGGILADSTKKYKTVILFGQIIMFAGYFLLAIPGLSLTISL
                     IGLFTIALGNGLFKGNLQAVVGQMYDNEQYSKIRDSAFMIFYMGINIGAFFAPFAATG
                     IRNWWLKVNHFLHDGSLPAICHQYINGTLKDASELQTLANRVSLSGPVTDLSAFAHDY
                     LEVFSTGYNYAFGIAAVAMIISLLIYVVFNRHLPNKEKVSVEKNSTIALNIQPLTVLI
                     ALLAGAVVAFLVSLVIDIGTGAAIGLFVAFVTWILIVSKKEEKPRVTALLLVFFVVIF
                     FWMSFQQNGLTQTFFARDYTVKTVDRVTYMFFTLENILTVIAFVAGLVLVFGRKRSRR
                     TRLIGGIILVVSGVIGYYLFQQGNDQNPISPEIFQSFNPLFIVSLTFVVMGVFDWLNK
                     KGKEPSTPRKIGIGMIVAAIGFSVLLIGSLNLVSPHELKFVDESGVIKYSPVPDYSRV
                     SPYWLIASYLILTIAELLLSPMGLSFVSKVAPERFQGLMQGGWLLATAVGSKLVFVGS
                     TFWGILDLWQLWLIFVVCCLLSAAFIFSIMKRLEKATS"
     gene            28918..32091
                     /locus_tag="PKX09_05270"
     CDS             28918..32091
                     /locus_tag="PKX09_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOU17266.1"
                     /translation="MTQIKYITGPLKSGKTAKVIQETVDVLRSGQAVGGILPSRDHID
                     YIKREILRSQPVLAPGQLFLGTFYAWAERILDECHYNFQTVQPAEEWLYLHDLLKQSP
                     VSNYHSFSGYIQILQDIFKDFRESGLTSAELELLAKNSGERELIEWVRLFNKLRQNYR
                     WCSAGPSGESLTIALELLKNKSNDKLGELLVIDGFYEFTPIQHKILQALTPLFTKTIC
                     TSIFDPSHPIYEYCQNRPGWLGSGETVMLSERTISSDPLRFLNSKLFKELIKHDNFTP
                     PNAVSNWKNEWNTPVKIVQCPSRRSEIETAARTIKRWLSEGMNPAKIGITFRGSYDYR
                     SLIKLIFPNFGIPVAMEVQNLLLSEPAQLITRIVNLNRENFSRPVFIDLLRLPVLQRF
                     YGADLLQELEIYSSEWGLPSVKSDWTARCRQQIEYLQFVLQIDREENDRERRDAQLQI
                     KRLQAIQPILTRLIEELSLPESAEWSDYHYLFIKILERYYPPDQPDEAQKILPIKSIL
                     ERLVRLRDFHTKTSLEELAGILPHFLDTGENEATLSSSGILCANLMDARAWDVDGLIL
                     LGLIDGEFPAIHPENPLFRKDLRSRLNQLANQPIFAEAAFDLAEEKFLLYLLTTRVNQ
                     RLLITYPESDNKGRALPVSPFIDEILACQPANTNQKIVEWEAIPAGQVLPDLINCSSD
                     SDLLQIVLAEKTAREDIEKLVSLIDRKIFDELAISLNCERRRLAGDGIRNGVLPEFRF
                     YPEFLAKPLSTTKIQNYVDCPFAYLCAHIWNIEISREPQIGLDALTEGLLIHKVLEQF
                     VQDFLSGEPQSWSDFLEVADDSFLNGLIARIDTLFRPKLYFVPDLLWEQVLNNLMMGL
                     RNFVEEERHYCPTGFAPILTEEMYALDNAFLIGNDPTLPVTITGKIDRIDHNASGKRF
                     LVIDYKRSSSSIIDIVNGALSGQQLQIPLYLLILLKSRPAEQVGGAFYYSFNDGKRTR
                     GFLVDVKFECTPELGSSDLNELLNKTQENVTAILEQIYRGNFNLLRRNENRCRGNECD
                     FYDLCRIASGQKNSHQQPATHSHQKRT"
     gene            complement(32047..33555)
                     /locus_tag="PKX09_05275"
     CDS             complement(32047..33555)
                     /locus_tag="PKX09_05275"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HOU17267.1"
                     /translation="MPIIIPFSTEWITFACFLGLILGIVGLSEVLRRSSRLTGEQTRR
                     FVHIGVGIFVIFSPLFFKSALQPALLALIFILLNAVALKAGQMKGMHTTERRSYGTVF
                     FPLTFLILIAMYWYSDPVILMLGMMVMTLADPLASIVGMKVKKPRQFTFWKDPKSVQG
                     SLVVFITTFIIITAFLPLFRWLDGTPIPNWQSLIVMAISVGVVATLAEAISSAGSDNL
                     SLPLSAALMIDVMQSLTFQGQIAVMGWVLFSFLLAYTAYRLKALTAGGAAGAMFLGSV
                     VFSIGGINWVIPMATFFVLSSILSKLGKAHKSQLAIMQEKGSNRDLIQVYANGGVALV
                     MAILYFYTEMDLFYLMFLGSLAAATADTWGTEIGTFSKSQPVHILTGKAVPAGTSGGV
                     TVLGTSGVFVGAGILTLSGIFPLEQSLMKIAVIILISGILGALIDSVLGGTIQAQYRC
                     PVCRKTTEKVKHCNNQATTLISGYRWINNDVVNLVCTLSGALLVGVGCWLLM"
     assembly_gap    33637..33736
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <33737..>35463
                     /locus_tag="PKX09_05280"
     CDS             <33737..>35463
                     /locus_tag="PKX09_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOU17268.1"
                     /translation="FCLRILRDNIGELPFDPLFKICDETAEALLRDQFWQKFLERKIA
                     ERDPDLTILINSITLDELSKLLDLIYEKQAECAPILERYKNLNVTSHLADIEKACRDY
                     NFQLLYEYFSDTTISDIINQISTFKSSKSEDVLQRDFCIILDASAEVLGRRQIPVSHR
                     DGSLRKALGGGKKGTAAAWGNELETVRELHSQLAAKFRLIKDEFHTFDPEIEKLHADV
                     MQSLARLALDFLQEYRAEMRRKALLDFTGIELETENLLARRSPEIVRYIRNFKHLLVD
                     EFQDINPIQYRIIQLLQELNPDLITFFVGDEKQSIYRFRGAEVEIFNTLREQSDVQPL
                     SINYRSVQALMNFYNFFFAQFLGSTPPAERYAAHYPLPIAAKDNSISTEPPVELLVIE
                     EDEEFAARPRQESPKDELLEARAIADHIWQLHKAEIIRENGVIRGAEFGDMTILLRSR
                     THQSQFTEALRQAGIPFYVVTGIGFYDQPEIVDLINYLRVLLNWHDEVALVGTLRSPL
                     VGLNDATLTELAREESLWKGINQYLKNGKEFVLEADEAARLKNFYHNYTELNKQITQL
                     STAEIINQIV"
BASE COUNT         9859 a         8045 c         7411 g         9948 t
ORIGIN      
        1 taatccagtc cggcttatca cggcaagcgg ctgtcaaatc ggttgaaaaa ttctgaaaag
       61 gtacggcctg cttagcctga gctaaacgtt ccggactacg attccagaca aattctggtt
      121 tgaacccaag ccgattcaga tgataagcca gcgaaacgcc aactcgactg gctccgaaaa
      181 gattaaacct tttccccgca aaatcattca tattggatcg ccaagaaata tccgctgact
      241 tttcgatttg gaatcatcgg catttttgaa tagaacgctc ccattatctt gcgaatttgg
      301 actgtatttc ccttcatcta ttccaccgct tcatttggtt taaagtaaac agtcgctagc
      361 taaataacaa gtccgttaac cggttaaaat cgtcacattt ctaaaacggt tgtcgcggaa
      421 ctcaattgcc tggcaagcca gttccagaca tttttataac aaaaaacctc gaatattacc
      481 actttgtcct catagatttc ctgctgaaca gtggtgtggt tgcgaattag agcgcagtcc
      541 gacacttcct caaacggagt ctgaattcgc aggtgacgac cttcccgcgc aatggctcgc
      601 tccaaagcct ggatcaaatc gtcaattcgt agttggttca acgccgagat gaataccgct
      661 tcaggataag ttttttgggc aatcttgaag acattttcat ctaatcggtc aattttattg
      721 aaaacaatta acgatgggcg gttaccagcg ccgagttcgt tcaaaacttc ttcgaccgac
      781 accaattgct gaagacaatg cggtgaactt atatcagcga ctttgataat caggtcagca
      841 tcaataattt cccgcaaagt actgcgaaag gaagcaatca ggttgtgagg taatttgcga
      901 atgaatccga ttgtatcgct taataaaaca tttggatagc cgtcgagatt cagctggcga
      961 accgttgtat cgagagtggc aaaaagttta tcttcgacta aaacatcagc accggtgaaa
     1021 agattaagaa tcgttgattt gccagcattg gtgtaaccgg ctaaggcaat cctaaaacag
     1081 ttcgcacggc gctggctctg aacggtgcgc tggcgatcaa tagcaaccag ttcgcttttg
     1141 agttttgcga ttcgagtctt gattaaacgg cggtcggttt caatttgagt ttcgccggat
     1201 ccggctcgga cgccaatgcc cccgatttgg cgttccaggt gcgtccagcg ccnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntggctttg ccccggccga tgaaataggc
     1381 tggatcaacg gctctttgat tcacaaattg cttatcagcc atttcataac cgagagtatt
     1441 cattagtaat tctaattcag ttaaatgttc ttcggttata tggcgcggct gattggaatt
     1501 aattatgccc accaggagaa ctttttcaga tggtagaacc gcgccggttg gaaaaatttt
     1561 agattgatcg ggcaacttgc tcattcggca ggtggattta ccatttcgat tcgatttaac
     1621 cgaccgataa acgtttctgc aatagctaga aacagagcgc aaaacaatag caacatgcta
     1681 aattcgcggg tcaatgtcaa ttggctgatt tcatcagtac tggtttcact aaccacggta
     1741 atattcgccg gagcattttt aatcaaattt ttataatcgg ctacgctaaa aaattcagcc
     1801 gctgtttcac tgagcggcaa attgacggct aatttttcaa tcataccgcc ttcccgaatt
     1861 acggaataaa ttccaatttc ggctgtatta tcaaatgaaa aataattaga cacaaccgac
     1921 gggttgaacc gacgtccgga aggagtttgc acctgaggga tggaattttc accgggaatt
     1981 ctggcgattt tgaccgtgtc tccgtttgtg taattcaagg gctgagtagc acctccagca
     2041 atatattgag tcatacgttg cagaatgacc gggaaaaatc cggaaaatgg caggtcgctc
     2101 cagtccgcct ttggaacggt agccatcatt accgctcgcc cggcttcgta atgtgtttca
     2161 accattagcg gtgtattatc atcgaaggac atcaatattt tatgctttga tccgcagatt
     2221 aaaccaggcg ctttaaaaaa acgcggagtg gcggtcggat taacattttc cggccacaaa
     2281 tcgcgaaaaa tcgggtggtt ggagtcatat ttaccaaccc cgagatagcc gtcatcaccg
     2341 gcttcccgag tattatgcca acgcgggaga tcgaagcgtt ccgcccagag agcgttgaat
     2401 tgctcaggcg tagagttacg agcaagaaag attaaaatac cgccgccatt ctgcaagtac
     2461 tcgctgaggt ttttctggct gactgaactt agactgacaa cctcggagaa aattataccg
     2521 tcataactgc tcagaatttc attaccgaga tattcgctgg tagtaaagcg ggcttcaatc
     2581 atctgatcgg gattggcttg caatgctttc ataatataat cgccgtcggt tgcttgacta
     2641 cccacgacca aaatgcgtaa agtcgcagga atattcaaga tgaagaaacg ccgattgtca
     2701 atcgccagat catcatcctc aatttgtaaa tagccactca aaatcccgga attcagcgga
     2761 atgaactcaa aagtcgcctg ctcgcttttc gcgggaggca aattaatcag cgcctgagca
     2821 actcgttcct cttcaataaa caatgaaacc agattttctt gaggttgtcg tcgccaatta
     2881 attgtggtgc tttgcaaggt aaccggtttg tccttggcat aaatttgttc gttaaaatgg
     2941 atcgcgccca gcgccgagtt gtccgcttca ccagtaaccg ggaaaagcac taaccggatt
     3001 ttattggcga ttatttcggc cagtagtttt tgctccattg gcgattttct gttccagttg
     3061 ttagcctgga agtcacttac gagccagatg acaccattca ggtcttggtt tttaattata
     3121 tcctcgactg ctaaagcaat tgccggaagc aactgtctgg cgaaattagt ctccggggta
     3181 tgaaccaatt cgcgctccaa atcagagagg ctgtaaaccg tagttcggtt gctgattcca
     3241 gctgtttccg tggcaaggat caagttcaaa ttaaccggga aatcaatatt tttagcgctc
     3301 gataggattg cctctatgcc tctggataat tgcgtccgcc cgtcacactt caaactcatt
     3361 gaagccgaat tatcaacgat aaggtataga ttagcacctt tcgccatata aattccaggg
     3421 gtttgactac tacgatacgg tctggcaaaa atcagaataa ggcataaaat cagcagagtt
     3481 cggataatta gcagaataat ttgtcgaatt ttcagacgac ggagaacgtc agcctggaga
     3541 ctttttaaaa aacgcagact gctaaattcc accacctgaa atcgttgacg accaagtaga
     3601 tgaatcagca aaggaatgcc agccaacggc aagagccaga gaaatcccgg atttaaaaag
     3661 gacatctcaa ctcctgcggc tggaatttat ttccgtgaag taaattcaat tcaggttttg
     3721 tcttgcaaaa gggcaacgat ctggtcagcg gcagtattga gaggcaattc gcgctgatcg
     3781 ccatttttca tatctttgaa aatgactata ttgcgggaaa tctcatcttc gccgataatt
     3841 actacccagt tagcttgttc gcgatcggca tcccgcaact gagctttgat cgaacgacgc
     3901 aggaaatcca tttgagcatt gacctttttg cggcgtagtt cggtcaccag tcgaaagccg
     3961 gccgttcgag agttttcact cattgtaata acgtaaacca agttgtccgt cggttcggga
     4021 aagctcccta cctgttccag aacaagcaaa agacgttcta taccggacgc cactccgacg
     4081 cccggagttg gcttgccacc gagatcggcg atcaggtggt catagcgacc accgccacaa
     4141 agggcatctt gagcacctaa gagaggcgag gtaatttcga aagtggtccg cgtgtagtaa
     4201 tccaaaccac gcaccagcgc ttgatcaatc gtatagggaa cattactaac gtcgagtaaa
     4261 tgacatactt ttttaaaatg tagtcggtcg tcattgctca gataatcgag tatcgaagga
     4321 gcatgttcag ccataatttg ccggcagatt tcgactttac aatcgaaaag acgcaggacg
     4381 tttttctgga gacgttcctg gcaagcgcca caaagttcat cgtgataagg ctcgatagct
     4441 ttgcgcaaaa ctatcaaata ttggccgcgt gttagacgat cgcctatgct attaagtcta
     4501 atattaagtt cagtcaaacc taattcgcga tacaattcat aaacggtttg gatcgtttcg
     4561 gcgtcggctt ccggatgcgg agagccgatg atttcaaatc cgaattggtg aaattgtctt
     4621 aaacggcctt tctggggccg ttcctgccga aaaagagaat ctatgtaata gactttagtc
     4681 agcggggcaa tttgctctaa attattctgg atataggcgc gcataaccgg cgcggtgagt
     4741 tcgggtttca gggtaatact ggtgccgcct ttatcgagaa aagtgtacat ttctttggaa
     4801 actacatctg tatcgccgcc aatactgcgt gtgaataatt ctgtgacctc gaaagctggg
     4861 gtacgaatct ccttgtaatt gttgcgttta aaatattcac gtaggaaatt ttccagaaat
     4921 tgccattgat gaacttcaga aggaagtata tcgcgggtgc ctttgacacg ttgatacaac
     4981 atattgatta tcaactcata ttttgcttta agctggtcaa cctgtggtac tcatagattt
     5041 aagaatatcc agagcagttt tcttcttacc acagggcaca aacaatttgg cgtaggagcc
     5101 ggtcgcgccg gtgctttgca gagaaaaagt agtatggagc gggtctattt tcaggatagt
     5161 ttcaatacca ttattttcga gagattcttt gaccatctcc gccattacga tgtccgataa
     5221 gacaccgaga caaaaccact tgacatcagt cattttctct tctggaaggc gggcgaccaa
     5281 ttcagcatta caatcaatgc attttgaggt gccctcgacg tattcacata aacaacgggg
     5341 acaaaacggc atttctaacc tctatacttg agaatcatat ttataaaaaa tatcggcgat
     5401 ttcttttgcc gatttagcgg ctaagatttc cgctcgacaa ttggcattat tcagcatacg
     5461 cgataatttg ctgagcaatt tcaggtgctt gctgggaaat ctctcgggag tcaagagaag
     5521 aataaaaata tgcaccggtt gggcatctat cgaatcgaaa tcaatgcctt ctggcgatac
     5581 accagccgca accaacactt ccattgtttc gcggtatttg ccgtgcggca aagcgactcc
     5641 cttgccgatc ccggttgtca ttaatttttc gcgctcaatg accgcctgac aggcggcttc
     5701 cggattggaa attaagcctt catcgtataa ccgttccacc aatatgctaa ttacttcctc
     5761 cttcgtttcc gccgaaagtg gatagacgat caagcgcggg gtcaaaattt tatcaagttc
     5821 cattagactt tacttctcat tcaaaaatct taacaaattt atagtataaa gcatttttac
     5881 acaactttaa tcattccaaa aatataacgg atagtccaat tttcaaaaca gtcaaaataa
     5941 atggacaaaa tcatcggatt tagatcgtag ccgccgagtg caaaaattgc cgatcactgg
     6001 caccgataac ccgacctttc ctaactctca tttaatggca taacaattta gaccagccaa
     6061 ttttaggtga atattttaca atataatcag gttacttcgg tcaattaaaa accacctgtc
     6121 ttgactaaca gctccttacc ctgaattcta gtccaaaagt aatagaaatt taacttaaat
     6181 gactttgtaa accagaatgc cgaccatcaa aatcagggac acgataatca acgcaacgct
     6241 aatgttgccc ttcttaattt cttcccattc attaatatct tttgacagca ggttaaaaac
     6301 tttgatagct aaaccgatgc cgagcgagaa accgagcgcg gctacgagtg cccaaccaat
     6361 agccctaaga tacatcgtga aagtataaga ccaggaaaat gggtccattt gatacctcct
     6421 tttgagtttt attgattcta attgagtttt ttcttttcaa atatttattg ccaatcttat
     6481 gattcgatcc gtaattaagt tactaataat caattttttt tatcaactac ttttttccaa
     6541 tgagaccaaa ttaaagcagg gcaggattat gggagatttc ttccagtcgc cagtaatcga
     6601 agaaagccta agatatagga gggttctatt ttgattagaa aatgtattat caatcggatc
     6661 attcttctga tcgaatttca aacttctgct caatttatct agttccaatt tggtttagta
     6721 atttcggcct ttcaccacta gtttttattg ccaagttgaa ttattgtgtc taatttgcga
     6781 cgcaatttta ataaaacgat agagcatttt gaaaatgaaa ttaaaagctg aaccggagaa
     6841 aatatctgaa gcagttgaat ttctccaaaa acggttaaaa aatcagagtg ccaaaatagc
     6901 aatcgtgctc ggatcaggat tgggttactt tgccgagcaa ctcgaaatcg ttgaccgaat
     6961 tgatcaacaa gaaattccac attatccgac ttctacggtt gtcggtcacg ccggtaaatt
     7021 catcatttgt aacatctccg gcataccgtt gatcgccatc caaggacgga ctcatctcta
     7081 tgaaggacgc actctggctg aggtaacctt ttatgtcgaa attttagccc gcctcggcat
     7141 tcgctacttg attctgacca atgcggctgg tggaatagat ccaaaactaa aaccgggtag
     7201 tatagttcta cttactgatt atattagttt tacccaaata ccactaacgc cggatggtca
     7261 aagccaaaca gcttcgccct tttgcgctga aatgatcgct atggtacgag aaatcgctct
     7321 ccaaaataaa atcgaattgc acgaggggat ctactgctgg actctcggac ctagctatga
     7381 aaccccagcg gaaatccaag ccgctcgcaa attgggcgct accgtcgtcg gtatgtccac
     7441 tattcccgag gcggtcacag cccaaaggct tggcttgaaa gtgctcggta ttagcttagt
     7501 cactaatctg gcggctggca ttagtgagtc accgattacc cacgaagaag tccaggttac
     7561 agctaataga agtaaggaag tttattccat tttaatggaa aaaataataa ttgagttagc
     7621 aaaaaatgac tgaatttttc tatatttagc tcgatttttg ttttcctgaa tttgttcatt
     7681 taaaatacca taaaactgat gggacgcatc actaaacaaa cagaaactga actgcttagc
     7741 tggccgaaag cattacttta ctcgtttggg gtttatctaa ttttaggaat tatcgctatt
     7801 ttagtgggtc tatacttttc gagagattta ccttcaatcg agcaacttaa acaagtcaat
     7861 ccggaactgg ttactcgagt ttactcctcc gatggggtta ttttacagga gttatatact
     7921 cagcatcgca tatacgtccc gatcgaaaaa atcccaccgg ctatggtgca ggctgtcgta
     7981 tccattgaag actatcgctt ctataagcat tggggcgtca gtttgcgtga tttcgctcga
     8041 gcaatggtaa ttgacattat tacccttagt aaaaaagaag gcgccagtac tattactcag
     8101 caattagccc ggatgttgta tgagtcaatc ggttttgaaa agacctttgt ccgcaaaatt
     8161 aaagaattgc taactgctgt acagatcgag cgcatgtatt ccaagagtga gattatcgaa
     8221 atgtatctaa actccaactg gatgggacac ggcgtttacg gtattcaggc ggcggctcag
     8281 cgcttcttcg ataaaaattc tgagcaactc accccggacg aatgtgcttt attagccgga
     8341 ataataaaaa attccgtccg gttctcgccg atccgtaatc caatcaatgc ctaccagcgc
     8401 cgtaaccttg ttctgcatcg gatgcacaaa tacggttacc tttccgatga agaatatgct
     8461 ctttacaaaa atacacctct ccaggttttc aaaccggaaa taccacctga acgtgctccg
     8521 tatttcgttg aatacattcg gcaatggctt cagcgcgagg atgagaaact cgggattgat
     8581 atttaccgcg acggtttgtc catctatacc acaattgatt caaaaataca ggcggcggct
     8641 gattccgccc tgaccagaca tctacaatat cagcaaaatt tgcttaattc aaggttactc
     8701 agcaacccct ctgaattacg tgattttata aaagatacta ccatatccct tgcgcaagtg
     8761 cgcgatatga tccggggtaa tattccgatg agtcccagta tgcaagccag tctggtagtt
     8821 cagggagcac tggtggcaat tgatccggct accggacata ttttagctat gatcggtggt
     8881 cgagattttc gtgaatccga attcaatcgc gctactcagg ctaaacgcca accgggctcg
     8941 gctttcaaac cgattgtctt cgccgccgcg atagataatg gttttccggt aactactcaa
     9001 ttacttaatc agccggtggt tttgaatatg ccggacggta gccgctgggc accgaaaaat
     9061 tacgatttat caaccggcgg tcctaccact ttgcgcgaag gattggctaa gtcactcaat
     9121 ttaatttccg tacgcgtcat tcaagaacta atttccccaa cttcagtcgt cgaactggcg
     9181 aaacgtatgc acttaactac taatatttta ccggttgacg ctctggcgct gggcgcttgt
     9241 gaagtcatcc cgatcgaatt aacctcggct tttggaatct tcgaaaacca cggagtttgg
     9301 gttgaaccaa tcgctattac ccgcattgat gatcgttatg gtaatacgat tgctcagtat
     9361 aaaccgcgtc aggaattagt tctaagcgaa gagacggctt atttggtaac cagtttactg
     9421 gagtccgcta taaatgtagg aactggcgcc ggagcgcgca gtgtttatgg attccgattg
     9481 ccagccgccg gtaaaaccgg tacaaccaac aatttcaatg acgcctggtt catcggttat
     9541 actccctata tggtagccgg agtctgggta ggagtggaca atccggctat ttctctcggc
     9601 aatcgccaat ccggcgcggc cgccgcttta ccgatctggg ctaattttat gcgggaatct
     9661 cataatacaa aaggctggaa agctaagaat tttgaacgtc cggatggaat tgtcgaagtc
     9721 caaatctgtt ccgaaactaa actcctgccc tcaacctatt gcccgatcga gactgaagtt
     9781 tttgccaagc cgaccgtccc gaccgaacat tgtcaaattc acaatgaagt cggtaaaccg
     9841 cgccccaaag acaacgtcgt cttttaatcg cacaaaaact tcaccaatta agcaatcgaa
     9901 aactggtatt ccgcaatcta tattattagt ctaaaagaaa tcttctaaca aactcatctt
     9961 ttaggaaaat ggtcggacga tgcagaccgc taatttgcca ggtgctactt agaaaccgtt
    10021 cgatcatttt ctggtcacat atttctctta taattggata aaaattacct tctttgcgac
    10081 cggtaccgta gagaatttca cgctcgaaca gcaatttaag caaacccaat ccgaggctgt
    10141 accgactttc acgaccgttc aaatctttct ccagaaaaat ccggtctgat tcgatttcag
    10201 ggcaatgcgg tgttttttcg ttatctatca cctcacgcat aaaattcaaa atccggcaga
    10261 tccggaaaag agtcatagcc gctacccggc tacattcatc gctgtcaatc ggatagaaag
    10321 atgaacgata acatagaacc gacgaaggta ttagaccaag tctactaaat tgctgaaacg
    10381 acgccgtgcc tggcactaaa taaaacaaag caggaccaat caaaaccggc aatggcgcta
    10441 aaaaaagaat agattccagt aattcctccg cccgatcacc cggcaatccg ataataagat
    10501 aagtcgtgat gaaaaatccc aatttattgg cacattcgac gaccttgagg tattgctcgg
    10561 taccaaaggg gcgtccgact tgcctttgat gcgcttcaga aggagtcacg agagctaaat
    10621 taagcgagcg gaaacccgcc cgccacatag cagtcaaaat ttcatcatcc agattagtag
    10681 cggtgatccc gttcatcgct tgcagtgaca aattcaaacc ggtaaatcgt tccgctattc
    10741 ccttcagcaa agccagaaac cagtcacgtt caccgccgag atgatcatct tcaaaatcga
    10801 aagaacggaa tccttgagcg attttctcag ccatttcatt cagaacgttt tcaaccgagc
    10861 gcaagcggta gcgtgaaccc attgtctgat gtatacaaca gaaagaacaa cgatacggac
    10921 acccccggct ggttatgagg atagcgtgcg gtttcttttg ataataataa gtcggagtgc
    10981 ccggtgtttg ataatcggca gaaggtagag aattcaggtc ttctataaaa ttcaccggat
    11041 tttcttgtat tacacccgtt ttatcccgcc agacaaggtt cggaattggc gccaatgacg
    11101 gttggtcttt ttccaattca ttcaacaaac ggggtaaagc caattcgcct tcacctttta
    11161 tgacaaaatc agcgccttgt tctaaaaatt ctcgcggttg aatagtcgcc gccgcaccgc
    11221 ctacaatgat tttcgacttc agtacaattt cacggattaa ccggattaat tcaaaaaccg
    11281 tttcagcgta agttataaaa aggctggaaa ttagatagta atcgccatct cttggcagag
    11341 tttgtctgat ctccatctca ctcagtccaa agcgataata tctcttataa aggccaaaag
    11401 gggaagcgtc atccggccga taaaattgtc gaaccgaagc taattccggc ggcggcacga
    11461 tttctcgtcg cactcttgaa cgcaaatcca gcagattaat ttcatatttg ggtaaattcg
    11521 ccaccagcgc taataaaccc tgcggaatgt tccgtatcgg cgtcgtataa aaatcctcaa
    11581 taggcggttg aattaaataa atcttcatat ttccacttat aatctaagat aaaatggttt
    11641 ttctggctgt caattatttt tttgatattc tgcctgatgc accttaattc ccaaaagaaa
    11701 gtaattatcg ccaatctatt tagattaatg ataaaaagtc attgaccgat aaatgtcgat
    11761 cttattaacg tttctatttt tacaaaattg actgaattaa ggcgttcatt taccgacaat
    11821 aagcgcttga tgcaatgccc taactgctcc agtggcatac gtcggttcaa ttacaatact
    11881 cagagctaaa tcgttcgcaa aaacagcgag aataggaata tcaatttcgg ataatgtcac
    11941 ttcaaccttt tcaaaaagtg agggtcgcgc tcctaaacca ctgcccacta cggtaacgac
    12001 cgcgacttca ttagctgaga atccggcggc tttagccaat tgagccacac gaccggcatc
    12061 gcctcccgga atgccagttc gatcaaccag caaatcaaaa gttgcgcgct gatatagagc
    12121 cattacccgg ccaccagctt tattgatttc catcagtaga tcgggcaaat tcccgttgcc
    12181 attaatccga gagactttct cggctaaaac gatggcagtg gcacctgaac ccatcgcagt
    12241 attatgcact tttgtctcac gaccactact gtcaccggtt ttacgagcca gaatcgtgat
    12301 tccagccttt ttggcaaatt cgacagcgtc ggcattcagc acctttgccc cggctagcgc
    12361 cattgtttgc atgacctcgt aaggaatttc gcgcaataat tgtgcctggg gacagatatg
    12421 gggatcgcct gtataaactc catcaacatc cgagtaaatt tcgcaggaat cggcaccgag
    12481 cgcggcggct aacgcaactg ccgtggtatc cgaaccacct ctaccgaggg tcgtaatttc
    12541 cttagttgta gaaacgcctt gataaccggc gacgatcaca atatagccct tttcaagggc
    12601 ttcgatcaga cgaaacggtc taacttcaag aattcgagcg ccctggtggg tcggctcggt
    12661 gataatgcca ctttgactcc cagtgaaaga gatcgcgtct ccgcccagtt cctggatcgc
    12721 cattgataaa agagccatac taatgcgctc accggcggta accagcatat cgagttcgcg
    12781 ctgttggggg ttggcggaga cttcgcgcgc ctgggcaatc aaggcatctg ttgttttacc
    12841 catagcgctg acgaccacta ccacccgatt atctgcttgt tgacgccgca cgacaatttc
    12901 ggcaacctgt cgcagtcgcg tagtgtccgc caccgaactg ccgccaaatt tcatcacaac
    12961 ggtcttcata atcggttcga cgcgcttcct ttcactttaa tttatgattc agctgaaaat
    13021 tgcatttttc aacaacaggc taaaagtagc taacattctt tagacgatag caaaaggtgc
    13081 attgtttttt cacttactag ctttttcaaa aactgatcct tattagcgtt atttaactct
    13141 tattatttac cagattttca tacaattcta tatacttggt ggcggcttta tcccacgagt
    13201 aatcggcgct cataccgttt ataattatct tttgccactc atccggtttt tcaaaaacct
    13261 gcaaggcttc cgaaatggtc ttaagcaggg cttccgatcg atattcacaa aaagcaaaac
    13321 cattgccatc tttaccatcc cagggaatca ctgtatccac cagaccgccg gtatgatgca
    13381 ccactggcac cgcgccatag ttcaacgcat acatttgagt tattccgcac ggctcgaaac
    13441 gggaaggcat cagaaaaaga tcgcttccgg ctataattcg gtgtgataag atattatcat
    13501 agtcgaattt caccgccatc tggtcacgaa attgatttgc caggcgttct aattcaaact
    13561 gataatgctg ttcgccactg ccaagaatga taaagccgac gcctaaactt agaatttttt
    13621 ctaagctgtc aatcaaaaga tcaatgcctt tttgttccac caaacgcgaa accattccca
    13681 acaatggtcg cgattttagc gccggattta aacgcaatgt ttcaatcaat ttcgctttat
    13741 taattttttt cccagctaaa ttgctaatcg aataaggcgc aaaaagatga acgtccgtcg
    13801 ccgggttcca ttccgccgta tcaataccgt tcaaaattcc caccaccgga gttggacgat
    13861 tggctaaaac cacactcatt cccgccccca attcctcatc agtcataatt tcgcgagcgt
    13921 aggttggact aacagttgtg acagcgtcgg caaaaataat tccgcctttt aggggattga
    13981 ggctattata ccattctaat gctccaaacg gcttaaagag ttcttccggc aaatcgcaga
    14041 tttcctttaa ccgcatttca caaacacctt gataggcaat attgtggaga gttaggacac
    14101 tttttagcga attaaaaggc gttactttcg ccaaattatt tttaagataa accggaatca
    14161 aagccgtatg attatcatgg cagtgaataa tctcagccga ccagcgagtc tcggcaatta
    14221 attttaaagc ggctttttgg aaaaacacaa aacgttccgg attatccgga tatggctcgc
    14281 cattttcagc gcaatagaca ttgcgttgcc caaaatattc cggattatca ataaaataga
    14341 cctcgtgccg aatctgagct ggattttgcc agcgcctgac ctggctttcc accacgcggt
    14401 tacccagcgt gactgtaaga ttcgataatt gcgttaacgg ctcggtttta aagctccagg
    14461 tcgagcgata gcctgggatt atgactttga ccgaaagacc acgatttgcc agcaccggtg
    14521 gtaaagcgcc gattacatcg cccaagccac caactttggc aaatggagaa gcctcagcgg
    14581 caataaaaat aatattcatt ttgaactatt attagaatca acccgtccgg tttccggtgg
    14641 gagtttactc cagtaagtaa aatcgagttc cgggaaaatg tcgtctactc gttcgatacg
    14701 ttctaaatat ttttgatggg tttcggacag tcggccgttg ttttcataat catttaaaat
    14761 tctaacgata ttatggaact ggtcgcgatg cacgccgacg cgattctccg cataatcacg
    14821 cgcactccag gtgctgatca gaaattgcca gtcggaactt tccagcaata gcagttcgcg
    14881 ggctaagtca ttcaaaatgc gtcgtagatt gacatcgccg gtttcgcgat acttctcggc
    14941 taattcgacc attttatctt cggcggcata aatatgtttc caggtccatt cggtccattg
    15001 atttagccag atatagtgga agccaccctg tccccaacta ccttccggca tcgtaaccac
    15061 ctgagttgga cgatttttgg ctaaatgatt accgagtgaa attgcttgaa tatccggatt
    15121 ggcgttaatc ttacgcaaaa ctttaccgag ccagcgtggg ccttcgaacc accaatgccc
    15181 gaaaagctcc gtatcaaatg gcgccgttac aacgccgatt tttccgctct ccgcataata
    15241 tttcaaaatg tagtttctaa gtacttcgac aaaatgatca gattggtttt cgagggcttc
    15301 ttctacattt tctggcacat attcttgttt atcggctaaa tcagcctttg cgctggttac
    15361 tcgccaatag cgatgtccgc cagggaaatg tttcttatga aattccagat agcgaccgtc
    15421 gcccggatag ccccattcgc cgctccagac ctgcaaggcg gattcctcat gtcggccata
    15481 agccaccgct actcgtcccg tcccagtcga agatactaga taaggttggt aaatcgagcg
    15541 actcttttcc tcgggcggtg gcgtccagtt cttctcaaat tgttgccaga gttgcttcag
    15601 cgattcataa cgatcaatat aaacacctcg gtttttaccg cccagaatta gatgtttatc
    15661 aacaatgaaa tactgaatgt cgtatttgaa gaggaattcc tcgatgcccc gtcgtatgaa
    15721 ggttttggga tattccttga taggtgatga ccattgataa ctcggccggt aggcacattc
    15781 cggtaaccac attccgcgcg gatcacatcc gaaatggcgg cggtaagttt ccttgccgat
    15841 tcgaacttga gcatctatgg cttcatcatt gcctaataaa ggaagatagc catgcgtagc
    15901 ggcacacgta attatttcga tatgattgcc atcctggagt tttttgaaag cctggataat
    15961 attgcgatcc ggtaaggaat taaattcatc aattaagccc tggtaataat tttcccacat
    16021 tccagctaat ttttccagat gcagattgcc gaccgttgca aattcttctc ggttatcccg
    16081 ggcggctttg attttgattt ccagataatt aatgaaaccg tccttgaatc tttccgcggc
    16141 taattgctca gctaaaatcg gcgtgatccc gatcgtaatg ttcggactaa tgccatcggc
    16201 aattaggcta ttgaattcgc ggagaagcgg aatgtaagtc tcagccgcgg cttcatagag
    16261 ccaatccata ccgtgaggcc attcgccgtg attaataaca tagggaaggt gactatgcaa
    16321 tactaacgaa aaataacctt tcatctttaa tccttactta tcaattgaca aacggctaaa
    16381 aatacgacat tgccacaaga tttcaaactg cgggaaaaga atctaatcga gtttgtagat
    16441 tctttttatt cagcgaaagt ttgagagaaa taaaccaaac cgctatgagt tatctcagca
    16501 ttccaagcat tatctaaata cttgctatcc cattccataa gcccaaaaac agctatattt
    16561 atatcatttt acgagagccg gtggtactgt tttaggtgaa ccgggattaa acactttgag
    16621 ccaaatagta tattatgaga ccgacgagga aattattaag cgaattcaga atttaggtag
    16681 ccaggagaat ctcctgttcg ctaaactgcc aatctgaatg tcgtgccgat ttttcatcac
    16741 tgatcgcttg aacccaacat aatcggcgtc ggaaacatct cgatcaattc attaaaaatt
    16801 tacggaagac ttttgttcaa aaactattac ctgttgtaga tacctttgtc ctcgattcac
    16861 tacaaatcga aattcgtaaa tccgcctgac cgatagacat cagtcagttg ggtaggatcg
    16921 ccgatctcgc tatagcaaat tagtcgggca ggggcactgc cgacacagaa attttcaaga
    16981 aaaatacgaa ataacaaaaa tgaggatttg ctttttactt ttaacttttt actttttact
    17041 atgattagtg gcgagggacg gaatcgaacc gccgacacag ggattttcag tcccctgctc
    17101 taccgactga gctacctcgc cataatatca aagtgccaat cgtcttcttt aagagtgagg
    17161 tagcgaagga gaagactgcc tgtcccgccc ttgcggggct ttgtcccgct acggcgggaa
    17221 gctacctcgc cacaatatca aagtgccaat catcttcttt aagagtgagg tagcgaagga
    17281 gaagactgcc tgtcccgccc atgtgggact ttgtcccgct acggcgggaa gctacctcgc
    17341 cataattaaa aagcggctta atttaggaac tcaaatacaa ttccaaaaac tatttttcgt
    17401 tagtagttta tccgatttgg gaatataaca attactgtaa gttcaaaaag acctgttgat
    17461 ttataaaatt gctaacatta cttttataat tgtctgatag gagttcaaaa tgaaaatctt
    17521 cgacaataaa ggtatgttga tcattcccaa cccgatgcgt gggaaattta ccgaaaaacc
    17581 aaaattactg gtcgttaatc aattattttg tccgaacgtt cataacctca tcaaccaaag
    17641 agtcactttt aatggctacc caggtatttt aataaaagtt cgccaggatg aagcaatcgg
    17701 tttgattgcc ctcagcccat tttacggtga gtattcgcgt ttttcacttg atatagactt
    17761 gattgatggc aaattactga aattgatctg cccgacttgc gatgtggaaa tgccaatcat
    17821 tgcgcaatgt gagtgcggcg ggaatcttgc ggcttttttt cttactcgag aatgtaattt
    17881 taacgactgc gttggaattt gcacgcgggt caattgccat aattcgcgga ttgtgcatag
    17941 cggagagtta ataaccggct caatgcttga gtcgctttaa actgcttaaa attttaaata
    18001 attagtttta attcagccga tatgaaatta atcgctgata agccagcgcg taacgtttgc
    18061 cgaattctcg taaagaaggt gcattaaaat gcaaaccgtc atctttggac gccaatccct
    18121 cagccgagac gcaggcataa tccggcaaaa tgctttccag ttcctttaga actgccgaaa
    18181 tatccggtcc gtagggataa ccagttgcgg cgttgatgaa atcacccagt tcaccgctga
    18241 tgaaaggtaa gctgggcata tttaaatcac gccggaaaga ccacacgagg gaaactattc
    18301 ggtcgcgata tttttcagcc aaaattttat ctttggcgtc atcttcgccc tgatgccaaa
    18361 gaatagcgcc gagcatattt tcgtaagtat ccgtggctaa tgcggttttt actactgcaa
    18421 cagctcgctg atagtgaggc gtgcccggca accaatcttc aatcgccgta gaaccgactg
    18481 cacagggcag aagtccgaca ttttgcacta aatttctttt cagaatggag gaggcgaaac
    18541 tcataccgag ccctacaccc gccaggtctg cccgatcatc atgcaatggc tcaactgctt
    18601 tttgccagcg atcattccta aacatctgga ttcggtcatc atttatcggc agaacgcgat
    18661 ccatttcgcc ccgtcccgcc atgttagatt gtccaatcaa taaaaataag tgtctggtat
    18721 gcatcaggtt ttttccttaa tagagcgacg ttcagacgac cgctccggtt agattcgcca
    18781 caattcgaac ggcatcattg gcattctggc cgtaggcgtg cgcgccaatc atatcggcga
    18841 aactctgggt aaccggcgca cctcccacga cgatttttac ggcattatta cttttaaaat
    18901 agtcaacaat ctccttcata tacatcatag tggtagtcaa aagcgcacta cataaaagaa
    18961 tattgatccc ggcgttaact gcttcggcgt acttttctac actgcaattc accccgaggt
    19021 caagcacctc ataaccggcg ccttttagca taatgctaac caaattttta ccgatgtcgt
    19081 gcaggtcgcc ttttaccgtg ccgatggcaa ccttaccgcg cgctttgtga ttggacttag
    19141 ctagaatcgg ttcgattaag gtcaacccgg cttgcattgc gcgtgctgaa atcagcattt
    19201 ccggaacgta aatttcgttc cgcgcaaaac gttcgccgac ttcacgcata gctggaatca
    19261 tagtttcttc cagcaattca ttgacatctt gattaagatt gatggcattt tgcaagattg
    19321 cctgcacttt ggcgcgatcg cctttcaaaa ttgccagata aagttcttca ttacgtgcca
    19381 ttttttgctc cgaaatatac attatttcct atcaaaccac ataatttaaa atgttcgatc
    19441 actttggctt ggttcgagcc attcacaatg attgcaattt gcggttcttg cgcaattgag
    19501 gacgccggtt caatttgcca gccagctggc gaataatcca catattgata ttgatgactg
    19561 tcaaagagat aacctttcaa tcggtaaacg tcatcagcta ctgggatgat tgcttgacgt
    19621 agttgttcat agttcatatc gccggaattc agaattatcg tagcatatcg tgggtcgcgg
    19681 catggggcaa attcaccttc tggcacctgt aaacgctggg gattttccgg taaatccttc
    19741 ggcagttgac tgaaagttgt ccgatataca taggcagaag gattgacttg ccggattaga
    19801 ctttcagtcg aagctatttc ctccggtgaa gcaatatcaa ctttgttaat caagataatg
    19861 tcagccgcct cgatctgcgc ccgaatgctc gtcaaggtat ggaataattt gcccagactg
    19921 gctgggtcaa cgatatttaa gacttcccga atattataaa ccttatctag tccggcttcg
    19981 cttaacattt taaatatcac tcgcggatcg gctacgccgc tggcttcgat tactagctcg
    20041 tcaatttcat taccctcctg agtaaaacgc tggggaattt cccgtaaata ttcgataaat
    20101 ttccccgcca aacaacggca aaaaatactg ccgcccggga tgctaagata atcaggtgtt
    20161 aaattggcaa tgagtgagcc gtcaatgtca gtcggcgaaa attcattaac caaaaaaacc
    20221 agacgtcgcg actgattctg acggataaga tttttcaata gagtcgtctt gccgctaccc
    20281 aaaaatcctg tcactaatct gattggaatc atttatcttg cccagttttg aatttccagc
    20341 gatcgagaat agcggctttt agggtttgct gatcgggaat gatagaactg aatttcacct
    20401 cgccgtcaat acaaatggtc ggtaaacttc tcactcccag ctttttcatc ataccaattc
    20461 cttcactggt agttattttg tgttcgcgaa ctgttgtctt tatcgctaaa tcggcggtgg
    20521 ctttgagcgc ggcttcgacc atatatttgc agggagcaca ggtagccgaa tccagcgtaa
    20581 taatgtcaag aatcacttcc tcccgattgg cgtaatccgg taaaactatc caatctgctt
    20641 caaattcacg ggcggtcaat gttctgcgag cgacctcccg ctgatattcg tcatacacca
    20701 tttcagcaac ggcttgtaaa ttctgcggcg gaacattata aggtatatca cagccgggcg
    20761 ccagaatgaa acctacctgc ccgccttcat ctatattacg aatggcttcc agacggcaat
    20821 cgtccgctga acccattagc aaagcgaccg tcagttttag attgccaccg aaagatttat
    20881 tatacttctg agccagctcc cgcaatttag gaagtgaaat ctgctcatcc acggaaacgt
    20941 tgtcacaatt tgtttcacac atacatacca aattgcgatc cacatctccg catacaaata
    21001 gcgatgaaag tcctcccttc gctctgatat gatcgaaaat cggattgagc accggcgtta
    21061 cgaattgcct aaaatgctgc ggcgaaatct gactggtcat cggatcaaca accgcaataa
    21121 tatcggcacc gtttttcaga taaaaatcac tgacttgaaa agcaatttct ttacaataag
    21181 ccaataactc ctcaactttt tcaggatgat catacatttc gagaaaaatt tcattaccca
    21241 tcaggtgaag cgccagagta aaaggcccac aaatcaaacc gtaaattgcc aggtccttgg
    21301 caaaatcttg acgaataatg tccatagcct gggcaatctc tggatagcgg cctttagcca
    21361 gactaaattc tggtaaatct gatagatttt tgccattaag cgaatcgagg ggatgcgtaa
    21421 tgaccgacgg cggagcatcg tccgcccaaa gcagttcaca tcccagcacc tcggcttcca
    21481 attgcaggtc gaaaattacc ggcagtccat ccggacgata aagttcattg gcttttcgca
    21541 agccttcggc aattagtttg ccggacttaa ggtaattttc cgcggtctga ccgatcaatt
    21601 ttccgccatg acagcctaca aaaggcaccc agggtacccg agtggttttt tggcgattaa
    21661 aagcctgccg aaccaactgc agtccctgta ttgaattgtt ttccatttta ttcatatagt
    21721 aaatttactt tatattcaca tatcataaaa tcacatcttc gaggtaactt gtctttattt
    21781 tatcaattta ttgctgtata ttatcattat gaaaaatatc gttctgtcgc aagataaatt
    21841 cattcccagt cagagcggcg ccaatatcac tttccatcgc ttagcttggg atagtagcag
    21901 tcaaccagaa agcatcattg ccggatccga tctgcgctac tactctcttg ccagcgaaaa
    21961 tacagcgctt cattcccacg agtttgccga atttttcatc gtcatcagcg gcaaagtccg
    22021 gcatattgtc aacggtgaga cgcaggattt gcaagctggg atgttgactt tcattcggcc
    22081 aaacgacgtt catagcttcg agaaattaaa cgattcggtc tgtgaaatgg tcaacgtcgc
    22141 atttaaactc gaactgctcc tcgatctcag cacttatcta ggcaatgatt tcttcctgcg
    22201 acgttacacc ggaccggttt taccaccgac ctttaccctt tcactcgttg aaactgagga
    22261 attggctctc cgattgatcc gtctcataac gctccaaaaa actgatttag atcaggcgcg
    22321 tcttaagctc aaaatgatat tagctgaaat tttcacacgc ttttttctgg aatggcaaac
    22381 gcctgacccc agccgtcccc aatggttcga taaattatgt tctgaaatga agcggaaaga
    22441 aaacctgatc ggtgggctga aaactatgtt aaaactggct ccttgtactc aacagcacct
    22501 atgcaaatgc tttcgccaat atctcaacac ttcaccgact gaattcatta atgaacagag
    22561 aatcaaatta gccgccaaac aaataattga aaccgatgag aaaatccttg ctattgcact
    22621 ggaatacggt ttcaagagcc taagtcgttt ctataaaata ttccataaat actatggcgt
    22681 ctcaccagcg cgcctgcgtc aaattagtcg ccagaaagta attcccctct aaaaaaatag
    22741 caacttcgcc aacaggcgag tataataacg attcgatccg gttcgggtaa tcgcttttgc
    22801 tatttgcaaa attctcaaat tattttagag acttgcccgg ggaaataaaa tgaaatatta
    22861 atggaaattc aacactttga atttcaatca gttagtatgt attttatgat attaaattta
    22921 tgaaaaaatc agcctcgccg gtgagtaatg gcgggtgcga tgcagatctt ctacggctac
    22981 atatttaaag aaataatcat catattccgg gcggaagtca gcgagtgttg cgagatgaac
    23041 gactgttttg tggcgcgggt tgagtttttc gaacacggtg cgcagttgct catcattaaa
    23101 atctactaaa tggatcatat cgcagtcgag tttattgatt tctgcaaact ggatcggatc
    23161 attgacctcg acctcgattt ttattttttt gcgggcaaca cctaattcat caaccagagt
    23221 ggtaataacc tcatcagggg attttccggc ataaataaaa tgattctggg tcaggtaaat
    23281 ccgctgagaa atgccactaa acggcatttc gccaccaccg gtggtgaagg ctattttctc
    23341 aaattcttca aaaattgggg aaacgctgtt tccggcgact aattttttat tatgatcgcc
    23401 gaggaattta acgacctccc gcgttgcggt cgccagtccg gagagactgc tagccaacca
    23461 ttccaacaaa cgccaaattt taaggatttc agctccgttg ccattaaatt caaaaatagt
    23521 agcaccagcc ggtatttcat caccctcatt acaaagccat gcgacttgca gttgttcccc
    23581 ggcgctttct aataatttct ccattatagc acgccctgag aataatgtat cgcttgacat
    23641 cactaaccga ccggatgaat gtaaaggata gggggcgata ctctcggtag tgagatcgcc
    23701 ctctcctatt tctacgtgct tcatccagtt gaggatttgc tcaatcatct aattaatctt
    23761 caggctcgat ttccatatcg ccaacatcaa tgtccataac caagcccaat aactcgtcaa
    23821 tttctgaacc tagttttccc atatcatccc aaccgaagcg cccttccaac ctcttgatct
    23881 tttttacctt atgcataccg cagggctgtt ctttaaggat ttttctctgc tcatcggtca
    23941 gagtttttag aaattcgact ctttgatcaa ttttgttttt gaacagtttg gctttaatgt
    24001 cattgatttt tttgacactt tcgtcaattt tcttttgatc gagattatca atagcctctt
    24061 ttaaatccag acgagctagc tttaaatcgg cttgcaaagg gatgttcaag cgctgatatt
    24121 taaccataat ttttttatgg tcaactttct gctgatcagt cagtttgagt agatcagcca
    24181 gcggcgtttt acattgtccc tggcaatgca tttcatgcga ccctttcaga acttgttggc
    24241 agggttcggc tttaccttta acttccggtt gagctaaaat cagagccgga acagttaaca
    24301 ggattaaact taaaatgatg ctctttttca tttacacctc ctatttttgt tgtttttcta
    24361 ttttatcagt cgggggattg agggttttca ttaaatgtcg tcggaaacga ttatcgaaaa
    24421 tcatatattt caactgttgg ctaggggtta aaatcgaact caatccgctg atgaatttct
    24481 ctttttcggc gataatgtct ttttcgattt gggccatcgc acgtaaaaat ttcttaacat
    24541 cggcttcggt cacagtagta tcggtatttt gagtaatttg atcaatttcc cgaaccaatt
    24601 gcatctgttg atgttgcatt ttccgaacgt ttttttcgta ctgtttcaac ctcgggaaaa
    24661 atttttcgga ctgctccggc gtcaattcca aaaattcggt cattttatag atacggagag
    24721 tttccatttt ttcgcgtctt tctgccatac tagtttctga ttcgtcgaag gaatgcggct
    24781 gagccgccaa ttgaccggca aaacctagta gtattaatga cattgacaat aaccaattgt
    24841 ttttcatttg atatcctccc gcaaatttat aaaatggatt tttcaatttc ttctaggact
    24901 gattcgaaat cctgatcggt caaaaaggca gtggattcta tatagtcgtc aattagtaag
    24961 ttactattgg tagatagaac cacgctactt attttctgcc gtaattcgct ggtaatcaga
    25021 ttattctcgt taaagttcac aaagtcaaaa acatcttccg gcatttcgct aaaataaccg
    25081 gaatgggtca aatccatata aaggtcgatg ttgttatcat tggataacca ttgcgaggaa
    25141 atggattgat tacgcatcaa gttaaatccg ataatgataa aaattaatat tgaaccgagg
    25201 accggtacca gatagtactt taacttttta ggacgccgtt cattatggag tcggttttgg
    25261 atttttacta ctaattcagc tccataattt ggctcaatat tgacaggatc aggctgtaaa
    25321 tcatttatca ggtgttgtat ttcatagctg aatttgcggc aagcaggaca atcttttagg
    25381 tggttttcga cgagtctttt ggctgattca ttcaagtcgc cggtaaaaaa tagcggcaaa
    25441 taatctttaa tttgtttaca aattttcata agtcactccc attttgagaa atatctctcg
    25501 caggttttta acagcatgtg aaaaattcac cttggcggcg ttttcggtta tgttcaaaac
    25561 agccgcaatt tctttgaatg ataattcctg aaaagagcgg agaatgataa ccatttgctg
    25621 gcgcggtggc agtttggcga ttacccgacg taaaagatta cgattggctt gattgctttg
    25681 gaattcatcg ggttcggcgg ctgagtcagc caactcgagc cccagagttt gcctgatttt
    25741 catccgtctc aggtaattca acccttgatt gatggcaatc cggtaaatcc aggtttgaat
    25801 agaagactct tgcctgaatt tgggtaagtt tttataaact tttatgaaag tttcctgggt
    25861 caaatcgtcc gcctctgctt gactgcccac catgcgccgg aatagtccat aaatgcgctg
    25921 gtgattttca cgtaccaaat tgttaaacgc ctgttcatca tagactgggt ggttaacgtt
    25981 ttgtttagcc aaattgtata attccttttc acgttgcttt agacaaaaag cgcaagcgaa
    26041 aggttaaaca taaaatttta gataaaaaaa gatatttatc gaacgatatt acgtttgata
    26101 aaaagatcag cttttccgga aaaatccgga atctgaacct tattgaaatt cgataagaag
    26161 agctaaacta ttcttgtttt tcgtgcgttt cttttttttc agtccgcggt ggatgggcat
    26221 cagcaatacg gatagtccga ccttcgaact ctttaccgtc caaagcgtcg tgcgccttat
    26281 tggcttcttc aatcgaaccc atttcgacaa aaccgaagcc cttgccttta attacgttgg
    26341 cacttttgac ttccccgaat tgggcaaaca gctgggctaa ttgctcgtcg gtaacagaat
    26401 atttcagatt gccaacatac agtttactgc tttccattca aacctccata ataaattcaa
    26461 gtgtcagaga taatgttaat taacgcggag gtcgaagtga attgcactgc taaccccgac
    26521 aaggttacat tatttgacac ctatgagtat ctccctcgcg caggatgaag atctaatttg
    26581 gtataaatag ctaattctga atcaatcttt ccaaataacg cattatttta ataacaaaat
    26641 ttctgcaatt caaagaaaat ctgcaattaa ctggattttt attagaaaat aaacaggtaa
    26701 aattggtatt ctaactaaaa ataatcgcta tttcctaaat atcgcaacaa tattggctca
    26761 actttgataa ggttttccct tctactttta caggattact ttctattggc atatcttttt
    26821 ttcgccattc ttgataacct aagacgggac attcgtcatt aaattttcat tttatttttc
    26881 cgcctttaca cctatatttc cgtcgctatc ttatgactaa atatttaaaa tatttcatct
    26941 aagaggaggt tttcgcaatg ttcaagaatc atccgaaagg actggttgtc gcctttttta
    27001 ccaatatggg tgaaaggttc ggcttctata cgatgatggc gattctggtt ttattccttc
    27061 aggctaaata cggtatgaat gaaagtaccg ctggcaatat ctatagctgg ttttatttca
    27121 gcatttacgc gctggcatta ttgggcggca tactcgctga ttcgaccaag aaatataaaa
    27181 cggtgattct cttcggacaa attattatgt ttgccggata ttttttactc gctattcccg
    27241 ggctatcatt aacgataagt ctcattgggc tttttactat tgctctgggg aatggtcttt
    27301 tcaaaggaaa tctgcaagcg gtcgtcggtc agatgtacga caacgagcaa tactcgaaga
    27361 tcagggattc ggcttttatg attttctata tggggattaa tatcggggcg ttctttgctc
    27421 cttttgcggc gactggaata cgcaactggt ggttgaaagt caaccacttt ttgcacgacg
    27481 gcagtttgcc tgcgatttgt catcaataca tcaatggaac cttaaaagat gcgagtgaat
    27541 tgcaaactct tgccaatcgg gtgagtttga gtggtccggt gactgacctt tccgcttttg
    27601 ctcatgatta tctggaagtt ttttccacag gatacaatta cgcttttgga attgccgcag
    27661 tggctatgat tatttcgttg ctgatttatg tcgtttttaa tcggcatttg cccaataaag
    27721 aaaaggtgtc ggtcgaaaaa aattcaacga tagcgttaaa tattcagcca ctgacggttt
    27781 taatcgcctt attagcggga gccgtagtgg catttctggt aagcctggta attgacattg
    27841 gcaccggagc cgccatcggg ctatttgtgg cgtttgtgac ttggattcta attgtttcga
    27901 aaaaagaaga aaaaccgcga gttacggccc tgctactggt ttttttcgta gtgatatttt
    27961 tctggatgtc ttttcagcaa aacggcttga cacagacatt ttttgcccgc gactatacgg
    28021 tcaaaacagt cgatcgggta acgtatatgt tcttcacatt ggaaaatata cttacggtaa
    28081 ttgccttcgt agccggactg gttttggtat ttggccgtaa gcgcagtcgc cggactcgtc
    28141 tgataggtgg aattattttg gttgtcagcg gagttatcgg atattatctt ttccagcaag
    28201 gcaatgacca gaatccgatt tcgccggaaa ttttccagtc tttcaacccg ctttttattg
    28261 tatcgttgac tttcgttgta atgggtgttt tcgattggct caataaaaag ggcaaggagc
    28321 cttccacacc gcgaaaaatc ggcatcggaa tgatcgttgc cgcgatcggt ttcagcgtac
    28381 tgctaattgg ctcgctgaat ctggtttcac cgcacgaatt gaaattcgtt gacgaatccg
    28441 gtgttattaa atacagtccg gtgccggatt attcccgagt ctcgccctac tggctgattg
    28501 catcttactt aatccttacc attgcggaat tattactgag tccgatgggg ctatctttcg
    28561 tgtccaaagt cgcaccggaa cgttttcagg gactgatgca aggtggctgg cttctggcga
    28621 cggcggtcgg cagtaaactt gttttcgtcg gaagtacttt ctggggcatt ctcgacctct
    28681 ggcaactgtg gttgattttc gtggtttgct gtctgctttc agccgctttt atattctcta
    28741 ttatgaagcg actggagaaa gcgactagct aaacttctat ttgtaaaaat ttcagctgca
    28801 acttcggccg ctttgcaaca ttcccgcaaa acggcagtaa gttggcgaca aattctggca
    28861 aataatcgcg ttttgaatta cattcgttgg ggctgaaaag gatttgagat taatcatatg
    28921 acgcaaatta aatatatcac tggtccactg aaatccggca aaaccgcaaa ggttattcag
    28981 gaaacggttg atgttctccg ctccgggcag gcggtcggtg ggattctacc ttcacgcgat
    29041 cacattgatt atataaaaag ggaaattttg cggagtcagc cggttttagc gcccggtcag
    29101 ctatttttag gtactttcta cgcctgggca gagcggattc tggacgagtg ccattacaat
    29161 tttcaaactg ttcaacctgc tgaagaatgg ctttatctac acgacctttt aaaacaatca
    29221 ccagtgagta attatcactc attttccggc tacatccaga ttttgcagga cattttcaaa
    29281 gattttcgtg aaagcggatt gacatcggca gaattggaat tattagccaa aaattccggc
    29341 gaacgagagt tgatcgaatg ggtccggtta tttaataaat tacggcaaaa ctatcgttgg
    29401 tgtagtgccg ggccttcagg tgaatcgctc acgattgccc ttgaactact taaaaataag
    29461 tcgaatgaca agttaggtga gttactggta attgacggct tttacgaatt cactccgatt
    29521 cagcataaaa tcctgcaagc tttgacgccc ttatttacca aaacaatctg caccagcatt
    29581 tttgatccct ctcatccgat ttatgaatat tgccagaatc gtcccggctg gttgggttcg
    29641 ggcgaaacgg tcatgctttc agaacggaca atttcgtctg atccgctaag attcctgaat
    29701 tcaaaacttt tcaaggaatt aataaaacac gataatttca cgccaccaaa cgccgtaagc
    29761 aattggaaaa atgaatggaa cacgccggta aaaatcgttc agtgtcccag tcggaggtca
    29821 gaaatcgaga ctgccgctcg cacgatcaaa cgctggttgt cagaaggaat gaatcccgcc
    29881 aaaatcggaa ttacatttcg gggtagttac gactaccgaa gcttaatcaa attaattttc
    29941 ccgaattttg gaattccggt ggcaatggaa gtccagaacc tattactcag cgaaccggct
    30001 caattaataa ctcgcatcgt caatctcaat cgggaaaact tcagtcgccc ggtctttatt
    30061 gacttactgc gcctgccggt tctacaacgt ttttacggcg cggatttatt acaggaattg
    30121 gaaatttatt cgagcgaatg gggtttgcca tctgttaaat ccgactggac agctcgatgt
    30181 cgccagcaga tcgaatatct ccagttcgtt ctgcaaattg atcgggagga gaacgacaga
    30241 gagcgccgcg acgcccagtt acagatcaag cgcttacagg cgattcagcc catcctgact
    30301 cgtttaattg aggaactgag tttgccggaa agcgccgagt ggagtgatta tcattatcta
    30361 ttcattaaaa tattggagcg ttattacccg ccagaccagc cggatgaggc gcagaagatt
    30421 ctaccgataa aatcaatttt ggaacgactc gtccggctca gggattttca taccaagacc
    30481 agtctggagg aattagccgg aatcctgccg cattttctcg atactggaga aaatgaggcg
    30541 acgctttctt cgtctggaat tctttgtgct aatttgatgg atgcgcgggc ttgggatgtg
    30601 gacgggctga ttttactggg actaatagac ggcgagttcc cggctattca ccctgagaac
    30661 ccgttattcc gcaaggattt aagatcgcga ctgaatcagt tagctaatca accaattttc
    30721 gctgaagccg cctttgactt agctgaggaa aagtttctat tatacctcct taccacgcgc
    30781 gttaatcaga ggctattgat tacttatccg gaatcggaca ataagggacg tgctttaccg
    30841 gtttcgccat tcattgatga aatattagcc tgccaaccgg cgaatacgaa ccaaaaaatc
    30901 gtagaatggg aagcaattcc agctggacag gttctcccgg atttaattaa ttgtagttca
    30961 gacagtgatc ttctccaaat tgtgctggct gaaaaaactg cccgcgaaga cattgaaaaa
    31021 ctcgttagcc tgattgacag gaaaattttc gatgagttgg caattagcct taattgtgaa
    31081 cgtcggcgat tggcaggtga cggtattcgg aacggcgttt tgccggagtt caggttttac
    31141 ccggaatttt tggcaaagcc gctttcgacc accaaaatcc agaactatgt tgattgcccg
    31201 tttgcctatt tatgtgctca catttggaat atcgaaattt cgcgcgaacc ccagatcggg
    31261 ctggatgctc tgaccgaagg acttttgatt cataaggttc tggagcagtt cgtccaggat
    31321 ttcttatctg gcgaaccgca aagttggtcg gattttctcg aggtagccga cgacagtttt
    31381 ctaaatggat taattgcccg gatagatacc cttttccgac cgaagttata cttcgttccg
    31441 gatttgttat gggaacaggt tttgaacaat ttgatgatgg gattgcgcaa tttcgttgaa
    31501 gaggagcggc attactgccc gaccggcttt gccccgattc ttaccgaaga aatgtatgcg
    31561 ctggataacg ctttcttaat tggaaatgac ccgacattac ccgtgacaat aaccggaaaa
    31621 attgaccgga ttgaccacaa tgcttctggc aaacgctttt tggtgatcga ctacaagcgc
    31681 tccagcagta gcattataga tattgtcaat ggtgcgctaa gtggacagca actccagatt
    31741 cctctctatt tgctgatact tttgaaaagc agacctgccg aacaagtcgg cggcgctttt
    31801 tattattcct ttaatgatgg taaaaggacg cgcgggtttc tggttgacgt caaatttgaa
    31861 tgtacgccgg aattggggtc gtccgatctg aatgaattac taaataaaac tcaagaaaat
    31921 gttaccgcga tcctggaaca aatttatcgg ggaaatttca atctattgag gcgaaatgaa
    31981 aaccgctgtc gcgggaatga gtgtgatttc tatgatttgt gccgaattgc atccggtcaa
    32041 aaaaattcac atcagcaacc agcaacccac tcccaccaaa agcgcacctg aaagcgtaca
    32101 gactaaattg acgacatcgt tattaatcca gcgataacct gagatcagag tggtcgcttg
    32161 attattacag tgtttgactt tttcggtggt tttccgacag accggacagc gatactgcgc
    32221 ctgaatcgtc ccgcccaata cggaatcaat cagcgctccg agaatgccac ttataaggat
    32281 gatcactgca atcttcatca gcgattgttc taaagggaaa attccgctca atgtcaatat
    32341 accggcacca acgaatacgc cggaagtgcc caataccgtc acgccgccgg aggtgccggc
    32401 cggaaccgct ttgccggtca atatatgaac cggttgactt ttcgaaaaag tgccgatttc
    32461 ggtgccccag gtatcggcag tcgcggccgc taacgagcct aaaaacatta aataaaataa
    32521 atccatttca gtataaaaat acaagatcgc cataaccagc gccaccccgc cattggcata
    32581 aacttggatt aagtcgcggt tactaccttt ttcctgcata atggctaatt ggctcttgtg
    32641 ggctttgccg agtttggaga gaatagacga taacacgaaa aaagtcgcca tcgggataac
    32701 ccagttaatc ccgccgattg aaaaaacgac cgagccgaga aacatcgccc cggcggcgcc
    32761 gcctgcggtc aaggctttta aacgataggc tgtataagcc agcaaaaatg aaaacagtac
    32821 ccagcccatt accgcaatct gcccctgaaa agtcaaactc tgcatcacat caatcatcaa
    32881 agccgcactc aatggcaaac tcaaattatc ggaaccagca cttgaaatcg cctcagccag
    32941 cgtagcaacc acgccaacgg aaatcgccat cacgatcaaa ctttgccaat tgggaatcgg
    33001 tgtgccatcc agccagcgaa aaagcggcag aaatgccgtt ataattataa atgtggtaat
    33061 aaaaacgact aaagaaccct gcaccgattt cgggtctttc cagaaagtga attggcgtgg
    33121 ttttttgact ttcataccga ctatcgaagc cagtggatcc gccagagtca ttaccatcat
    33181 cccgagcatt aaaatcaccg ggtcagaata ccagtacatc gcaatcaaaa tcaaaaaggt
    33241 caatgggaaa aaaaccgtac cgtaagaacg acgctcagtc gtgtgcattc ccttcatctg
    33301 tccggctttc agagcgacgg cattcaataa aataaatatc aaagccagta acgccggttg
    33361 caaagccgac ttgaaaaata aaggactgaa aatcacaaaa attccgacac caatatgaac
    33421 gaatcggcgg gtctgttcgc cggtcaggcg actactccga cgtaggactt cgcttaagcc
    33481 cacaattccc aaaatcagac ccagaaagca ggcgaaagta atccattccg tagaaaacgg
    33541 tatgataatc ggcatttatt cttttctccc cataaaaacg ctcgaatttt atgcttttta
    33601 aaatttattt ccaaaagctt tgatgcttaa attagtnnnn nnnnnnnnnn nnnnnnnnnn
    33661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33721 nnnnnnnnnn nnnnnntttt gcctccgtat tctgcgggat aacattggcg aattgccttt
    33781 cgacccacta ttcaagatat gtgacgaaac tgcggaagcc ctcctccgcg atcagttttg
    33841 gcaaaaattc ctggagcgta aaattgccga gcgcgatcct gacctaacca tcctaatcaa
    33901 ttcaataaca ctcgacgaat tatccaaatt gctggacttg atctacgaaa agcaggccga
    33961 atgtgcgccg attctggaac gttacaaaaa tctcaatgta actagtcatc ttgccgatat
    34021 tgaaaaagcc tgtcgcgatt acaatttcca attgctatac gaatatttca gcgatacaac
    34081 aattagcgat attataaatc aaatttctac ctttaagtca tccaaatctg aagacgtttt
    34141 gcaaagagac ttctgtatta tcctcgatgc ttccgctgaa gttcttggtc ggcgacagat
    34201 accggtttca caccgggacg gctctttgcg taaagcgctc ggcggtggga aaaaaggcac
    34261 agccgcggct tggggcaatg aattagaaac cgtgcgtgaa ttacatagtc agttagccgc
    34321 aaaattcaga ctaatcaaag acgaatttca tactttcgat ccggaaattg aaaaacttca
    34381 cgctgatgta atgcaaagcc tggcgcgcct ggcattagat tttctgcaag aatatcgcgc
    34441 ggaaatgcga cgcaaagctc tactggattt caccggcatc gagctcgaaa ccgaaaacct
    34501 gctggcgcgg cggtcgccgg agattgtgcg ttacatcagg aatttcaaac atttgctggt
    34561 tgatgaattc caggacatta atccgattca atatcgcatc attcaacttc tgcaggaact
    34621 caatcccgat ttgattactt ttttcgtcgg tgatgaaaag caatccattt accgttttcg
    34681 tggggcggaa gtcgagatat tcaacactct gcgcgagcag tcagatgttc agcccctttc
    34741 gattaattat cggagcgttc aagctctgat gaatttctac aatttcttct ttgctcaatt
    34801 tctcggtagc acgccaccgg cggaacgtta tgccgcgcat tacccgctac cgattgcggc
    34861 taaggacaat tctatttcta ccgaaccgcc ggttgaactg cttgtaatcg aagaagatga
    34921 agaattcgcc gcgcgtccgc gccaagaaag tccgaaagac gaactcctgg aagcccgagc
    34981 tatcgccgac catatttggc aattacataa agccgaaata atcagggaaa acggtgttat
    35041 ccgcggggct gaattcggcg atatgacgat tctcctgcgt tcgcgcaccc accaatctca
    35101 gttcacagag gctctgcgcc aggctggaat ccctttttac gtggtgacgg gaatcggatt
    35161 ctacgaccag ccagaaatcg tggacttgat taattatctg cgggtgttat tgaattggca
    35221 cgacgaggtc gctttagtgg ggacattgcg gtcgccgctg gtcggattga atgacgccac
    35281 tctgactgaa ttagcccgtg aagaaagtct atggaaaggc attaaccaat atttgaaaaa
    35341 cggaaaagag ttcgtactgg aagccgatga agccgcacgc ctgaagaatt tctatcataa
    35401 ttatactgaa ttaaacaaac agattacgca actatccacc gccgaaataa ttaatcaaat
    35461 tgt
//
LOCUS       DAOSMP010000022        35364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_1236_length_35364_cov_19.261095, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000022 DAOSMP010000000
VERSION     DAOSMP010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 35364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35364
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1236_length_35364_cov_19.261095"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            358..1206
                     /locus_tag="PKX09_05285"
     CDS             358..1206
                     /locus_tag="PKX09_05285"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020001360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOU17269.1"
                     /translation="MPYLGCHVSIENSLEKAPERGRELGCDAIQIFTSNQMQWKGVPV
                     ADETAEKFLNYQAEHKIAIVIAHDSYLVNLASPEPVKLAMSRKAFIEEIRRSQALKIP
                     YIVFHPGSHMGAGVDFALRKIAESLDYAIEKTPDCRPMFLLETTAGQGTNVGNAFEEL
                     RQIIDFSAHPERLGVCFDTCHAYAAGYDLVSPAKYDETFARFDDVIGLSQLKCFHLND
                     SKKPLGSRVDRHANLGDGFLGWEVFYRLINDDRFGNLPMILETPGGDENFAKEIASLK
                     KALEKK"
     gene            1219..2613
                     /gene="lpdA"
                     /locus_tag="PKX09_05290"
     CDS             1219..2613
                     /gene="lpdA"
                     /locus_tag="PKX09_05290"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOU17270.1"
                     /translation="MDNFDLIVIGSGPGGYVAAIRAAQLGMKVAIVEKAELGGICLNW
                     GCIPTKALLKNAEVWQNLRTLDEFGITIAQPQLDFTQVIRRSREVAARLSKGIEFLFQ
                     KNKVTVVKGYGKLRDRQTVEVVNPDDNSTTGLKARNIVIATGARPRSIPNVEIDGKRI
                     ISSREAMILPQIPKSIIIIGAGAIGVEFAYLFNAFGVEVNLVEILPQILPVEDEEIAR
                     ELSRAFRKRQIGVHIDTKVKSVQTKGNFVEVALASGGESQILVADYALVATGVQPNSD
                     NLGLAELGVSTEKGWIKVDEHFRTNIPDVYAIGDVIGNPCLAHVASAEGKHAVEFIAG
                     KNPAPVNYSAIPSCVYCQPQVASVGLTEKRASELGYELKIGRFMFRANGKAVASGESD
                     GFVKVIFDGKTQKLIGAHIIGTEATELLPELVLAIDHGLTYDELKDSIHAHPTLSEAL
                     IEAVHDAFGESVHQ"
     gene            2688..3377
                     /gene="lipB"
                     /locus_tag="PKX09_05295"
     CDS             2688..3377
                     /gene="lipB"
                     /locus_tag="PKX09_05295"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOU17271.1"
                     /translation="MPSAVKILDVLDLGLCPYGEAWEIQKKLHAERLASQIPDTLILV
                     EHPNVYTLGKNADARHLLASQEYLKKRGIEVYQIDRGGDITYHGPGQLVGYPIFNLKD
                     HKTSIAWYVNSVEEVLIKTLVKFGIRAERIKGLTGIWLANQKIAAIGMRVSHWVTMHG
                     FALNVTTDLSLFGGIVPCGLATKGVTRMCDLNSAVTMDAVKAVIIKEFVAQFGFRESR
                     MKMNFNTSKPL"
     gene            3558..4394
                     /gene="lipA"
                     /locus_tag="PKX09_05300"
     CDS             3558..4394
                     /gene="lipA"
                     /locus_tag="PKX09_05300"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOU17272.1"
                     /translation="MKPDWLKVKIGVNDDFHDLQRIINTHHLHTVCQSAHCPNQSDCW
                     QRRTATLMILGNVCTRNCRFCAVASGKPTAIDEKEPAKVGQAVNLMGLKFAVLTSVTR
                     DDLPDGGAAIWAKTIGEIRRQNPDCKIEVLIPDFRGNIDALEIVLDAKPDILGHNLET
                     VRALYPIARPQADYEQSLRLLSEAKKRGAVTKTGIMVGLGESDEQIHELMQDAFTAGC
                     DILTIGQYLQPTKDHLPVARYVHPEEFKSYETFGLGIGFRVVFAGPLVRSSFHAAEIA
                     GT"
     gene            complement(4581..5891)
                     /locus_tag="PKX09_05305"
     CDS             complement(4581..5891)
                     /locus_tag="PKX09_05305"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOU17273.1"
                     /translation="MNFTFSIYYVPLILSIIICAVIMFHSWRLRRDSHGIAFFFLALG
                     CIIWAFCNIMEYGFNTLSTKTFWVSIEYIGIEMVIVAWFIVIANFTGQTKFTTRRSIL
                     LLCIIPIITVFLIWTNRYHGLMRRNITLDTSGPFSIIKKSYGIWFWVTFAYSNLLMIS
                     SSIMLMQRLIQPPKLRRSQILALMACALFPWVGNFLYVFNLLPAPWSRLDMTSTLLSV
                     SGAIMAFGLFRLQIMEIVPIARDCILENLTDAILVIDKRNRVADYNPACRNMLKLNEI
                     IIGQPVEEVLNPLAIRPELIAEIKNVQEEISFTFGNETKIYDLRILSLFKKKQKLCGR
                     IFHFRDVTDKRLAEMHLEKTVVDLRRALTEVRTLSGLLPICSSCKKIRDDTGYWKDVE
                     TYISQHSTAEFSHGICPDCMQKLYPEQYETLVKKGKISAKSNES"
     gene            complement(6036..7667)
                     /locus_tag="PKX09_05310"
     CDS             complement(6036..7667)
                     /locus_tag="PKX09_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17274.1"
                     /translation="MFVNFNSVTTIPAIPDWWFWLWPAIMYAEMHYRLKFLRGRLFKR
                     QPEILFDVPRRVQTTRLPLVLIIKDAHWFPVTLKQVILKITSPFDDNLCVEQVFIENL
                     KVDEPWFTREYSLEVAKYRNHQLSVNCTAILECDGRIITIQNDNYAGLSQADFSVYVD
                     SEPLPADSGWIWGDLHCHSQWTSDQVEFGVPLAVLPGLAKAMGLSFCGLVEHSYDLDD
                     LPDSWTKNDPELRQWFAYRQAVQAINAKLNDFIIIPGEEVSVDNGLGRNVHLAVLNNS
                     EFFPGSGDGLERSVGFASELTYQRVLERAESQALIFAAHPLCKPPLTQQIIARRGIWN
                     LHDQFQRLNGWQILNGQEGKEFSASRNFWIKKLLSGNHSYIYAGNDSHGNFNRFRQVK
                     IPLLLMHENDHQIFGEFLTGVQTDLATGLDDLVANLKNGAVIVSNGPFLNIKAIDSRQ
                     NRYAIGQTMPELPQSVEITALSTRHCGAIHSIRLYIGNFERQVEEIKTVEVAGGIYRN
                     IINFPVSNLPNSGYFRAEVETKNRKFALTNPIWFS"
     gene            7820..8380
                     /gene="efp"
                     /locus_tag="PKX09_05315"
     CDS             7820..8380
                     /gene="efp"
                     /locus_tag="PKX09_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOU17275.1"
                     /translation="MATTADIKNGLVINFNGDLVKVIEFQHIKLGRGGARVWTKFKKI
                     RTGQVIENTFRSGEKIDIVRLEAVEMQFLYRDGSDYVLMNTETFEQITVPEEIFKDAA
                     QFIKENELVKVTFFDGKPIDVEVPIFVNLKVVKTEPGKKGDTVTGASKPAVLETGYTV
                     QVPLFINAGDIIRIDTRSGQYCERVK"
     gene            8396..8878
                     /gene="accB"
                     /locus_tag="PKX09_05320"
     CDS             8396..8878
                     /gene="accB"
                     /locus_tag="PKX09_05320"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOU17276.1"
                     /translation="MRKEKILELVKILESHEIDEIEISRWGQKIRISKNSNTGISTGV
                     EVAPAPQKAKKGNKPQLAETITFESRTAVTEEINHKGVEVKAPIVGTFYRSPAPDAEP
                     YVNVGDTVAVGQPLCIIEAMKIMNVIEAEVAGKVTKILVENGQPVEYNQTLFIIEKTE
                     "
     gene            8900..10240
                     /gene="accC"
                     /locus_tag="PKX09_05325"
     CDS             8900..10240
                     /gene="accC"
                     /locus_tag="PKX09_05325"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOU17277.1"
                     /translation="MIKKLLIANRGEIALRIIRACKELNIPTVAVYSTADALSLHVRF
                     ADEAVCIGPPESKNSYLKMANIISAAEVTNSDAIHPGYGFLAENADFAQMCEDNEILF
                     VGPHPAAIRTMGNKAQARETVKKAGVSVIPGTDTVVKNIEEVKRFAESYGYPIILKAS
                     AGGGGRGMRIVRQIAELENAFNNAREEAQKAFGNPDIYIEKLVEDARHIEVQIIGDNY
                     GNVIHLGERECSIQRRHQKLIEESPSPVVDEEMRDKLGKIAVRAAKAVKYSSAGTIEF
                     LVDSKRNFYFMEMNTRIQVEHPVTEMVTGIDLVKEQIRLADGAKIPKWMKKFNLRGHA
                     IECRINAEDPTKNFRPAPGKIQAFHSPGGPGTRMDTHVYAGYEIPSYYDSLLGKLITH
                     GKDRDEAIVRMERALDETIIEGVPTTIPFHKAIIRNEAFRKGDFNINFLNNFSF"
     gene            10257..10634
                     /gene="gcvH"
                     /locus_tag="PKX09_05330"
     CDS             10257..10634
                     /gene="gcvH"
                     /locus_tag="PKX09_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOU17278.1"
                     /translation="MKVPANLKYTKDHEWARIEGAVAVVGITDYAQSQLGDVVYIDLP
                     KVGDVVARGDVIGTIEAVKTVADIYSPVSGEIVAVNSALKDASELVNKDPYGEGWIAS
                     IKLSDSNELDSLLAPADYEKLIS"
     gene            10638..11978
                     /gene="gcvPA"
                     /locus_tag="PKX09_05335"
     CDS             10638..11978
                     /gene="gcvPA"
                     /locus_tag="PKX09_05335"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOU17279.1"
                     /translation="MHFIPNTCDTERAILENLGVASFNDLIANIPEKFREQCRINLPP
                     ALSEIELTKTMQELSEKNGNSDKQVSFLGGGSYDHFIPAVVDFLISRSEFYTAYTPYQ
                     AEVSQGTLQAIYEYQSQICHLTDMEVANASMYDGVTALAEAAIMAANITQRKKFLVSP
                     LINPLARQVLETYLTARDFILADLPGKNGQTDLSVLKEMIDNQTAAVLVQSPNFFGII
                     EDLNYIAEIIHAKSSLLIMSVDPISLAILKTPGELGADIAVAEGQPLGIHQSFGGPYL
                     GVFTTRRQYVRNMPGRIVGQTIDVEGKRGYVLVLQTREQHIRREKATSNICTNQGLMA
                     LAATIYLSLMGKTGLRKVAELCLNNSHYLADKIAGLPGYSLPFAGQPFFKEFVVKTPR
                     PATEIAAAALAIGFFAGIDLGQYRREWENYLLIAVTEKRNLQEISDFVKILAKF"
     gene            11984..12736
                     /locus_tag="PKX09_05340"
     CDS             11984..12736
                     /locus_tag="PKX09_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HOU17280.1"
                     /translation="MGVLNQLLEKARLKGGGYFVLIDPDTRSTPELKAFVREICACGV
                     DAILVGGSLIIGGGFHETLDIVKTESDVPVIIFPGSVAQVSNRADAVLYISLISGRNP
                     NALFGEHVIAAPVIRKLGIEPISTGYMLFESGRTTTAEFMSNTRPLPINKPEIAMAHA
                     LAAQYLGMSTVYLEAGSGAERSIPNQLVKAVCDYIDIPVFVGGGIRTPDEARAKVEAG
                     ATFIVTGNVFDNDANIGLAKEFVRAVHVKEHF"
     gene            12749..13324
                     /locus_tag="PKX09_05345"
     CDS             12749..13324
                     /locus_tag="PKX09_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HOU17281.1"
                     /translation="MHQKIIAQLEESAEVKRQTIITAVDSIEAAAQMLIDCYRAGGKV
                     LLCGNGGSAADAQHLAAELISRLKLERAAIPALALTTNTSLLTAIGNDYKYDLVFVRQ
                     VEAFGRAGDVLIAISTSGESENVIKAVEFSRVQGIKSIALTGAKGGRLAEKVDLAIKI
                     PSDNVQRIQECHITVGHILCELIESALYGTI"
     gene            13340..14230
                     /gene="xerD"
                     /locus_tag="PKX09_05350"
     CDS             13340..14230
                     /gene="xerD"
                     /locus_tag="PKX09_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOU17282.1"
                     /translation="MISEIQDFINYLKVERNLAPNTIQAYSTDLERYSQFLTQQQIND
                     ISKVRFSDIQNYINVLAELGLAARSLARNYSSIRAFHRFLFSERITAQDPTELLQSPR
                     LPRRLPKVLEVEEIDSILNAIDCTVNKGIRDRALIETLYSTGVRVSELIGLKMGDIFF
                     HQGVMRVFGKGAKERVVPFGNRAAHDIKNYITAVRSLLIRYGKSRDFLFLSMRGAPLT
                     RMAVWNIIQEYVRLADIRKPISPHVFRHSFATHLLEGGANLRAVQEMLGHADIATTQI
                     YTHLDREFLIEQHRTFHPRY"
     gene            14230..14865
                     /locus_tag="PKX09_05355"
     CDS             14230..14865
                     /locus_tag="PKX09_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOU17283.1"
                     /translation="MNFQVIILLIPPILLALTFHEYMHGWTAYKMGDPTAKMAGRLTM
                     NPIAHLDPIGTLMLFLVHFGWARPVPVDPRYFRNPKRDMLIVAAAGPLANMFIALLSG
                     LLARLFQGGYFSFLPLSVLQPLYVMVILSLQINLGLAIFNLLPIPPLDGSKILMGLVP
                     PRYEAQFNWLERYGSFILMAVIIVGMVTNFSILGAFINPFVNFFSQLFGGI"
     gene            15101..16162
                     /gene="ftcD"
                     /locus_tag="PKX09_05360"
     CDS             15101..16162
                     /gene="ftcD"
                     /locus_tag="PKX09_05360"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOU17284.1"
                     /translation="MTEIIECVPNFSEGRDHDVIRAIAVAIEKVKDVKLLDVNSGASV
                     NRTVITFVGNRESVINGAFAGIARAAELIDMRHHRGTHPRLGATDVCPLVPISGISVE
                     ECIALSHQLAKCVAEELYIPVYLYEKSAQKPERIDLANIRAGEYEGLKQKLSDPNWRP
                     DYGQPIFNPKSGATIIGVRNFLIAFNVNIDTRDRQQANDIALTIREKGRIKRDQNGNK
                     MYNSQGKPIYETGLLKNVKAVGWYIDEYKMAQVSTNLTNYKVTPPHVVFEEIRRLANA
                     RGLRVTGSELVGLIPKEALLMAGRYYLKKQGYSEDAPESELIQMAVQSLGLNAIAPFD
                     PAKKIIEYRLAEYEKQLTS"
     gene            16595..18412
                     /gene="mutL"
                     /locus_tag="PKX09_05365"
     CDS             16595..18412
                     /gene="mutL"
                     /locus_tag="PKX09_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOU17285.1"
                     /translation="MSKIKILSENLTNKIAAGEVVQRPASIVKELVENSIDAGADEII
                     VVIRNGGKTLCEVVDNGEGMSRDDLLLAFERYATSKIATVEDLTNIHTLGFRGEALAS
                     IAAVAIVTAISTLRGANEGYELRIEGGTFRDIRPTSPKPGTTISVRNLFYNIPARRKF
                     LKSAEVEFRHIVDVMQEFALIHYDLRFIFIHNDREIYNLSPSSLAERIANLFAPEYSQ
                     NLLPVESVADDLKVTGYIGNLNLVRARRGEQFLYINRRFVNDRLMNHAIISGYGGLLA
                     RSEFPFYCLNLELPPEQIDVNVHPTKMEVKFREQDRVYQFLKQATANSLRSITNVIPD
                     LGTFAPHHYYTTPPPPKGTQIQIPAPDQSPSKQSEFDSSRKTFDVPDVEIPPTPFAAG
                     DSTKWQERARRFVEQGETSEADDFRPSVAVYQVHNKYIISQVKSGLVIIDQHVAHERI
                     LFEEAQESMRGENWKAQQLLFPVVVELTVNDFSTLLEILPFLEKLGFRMREFGKQTVA
                     VEAVPAGLRWGNENIIIREILDHYQEFGTKDTSIQDKVAASYACKAAIKSGDPLTTEE
                     MQNLVDRLFATSNPYFCPHGRPIIINLSLKELDKRFERA"
     gene            18416..19396
                     /gene="miaA"
                     /locus_tag="PKX09_05370"
     CDS             18416..19396
                     /gene="miaA"
                     /locus_tag="PKX09_05370"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOU17286.1"
                     /translation="MSANSNPTNEIPSRKIPFIIGPTAVGKTATAIELAKQLNGEIIS
                     VDSRQVYRGLDIGTAKPTLRQQQEIPHHLVDIVDLDEQISAGAYRKLALKTVSEIRSR
                     GRLPIFVGGSGLYVNAVLKGIFNESTTNPEIRREIRQELQEKGVVALYNQLLEIDPDT
                     VLKIHINDVKRITRALEIYRITGQPPSCHFQKQDRRPPFAYRIFILNADREELYRRIN
                     ERVEQMLTDGLVAEVQKLIVAGYRSKLESLRTLGYQEVLIYLDEKCSYEEMRENIKQN
                     TRRYAKRQLTWFRNQYPEAVWIDVTELSNPLTIANTIRQNLKQTEIQSQA"
     gene            19628..22120
                     /locus_tag="PKX09_05375"
     CDS             19628..22120
                     /locus_tag="PKX09_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007760158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOU17287.1"
                     /translation="MIFPKFSILLAFALIISSCAKKPDRNSDSANNPMKLWYQQPAKQ
                     WVEALPIGNGRLGAMVFGDPRLEEIQLNENTIWGGKPHRNDNPEAKEALPKIRQLIFN
                     GKYKEAQELADQKFISKNSQGMPYQTAGSLYLSFPEHKNFTNYYRELNIETAVVTTRY
                     EVDGVTYKREVFASFPDQVIICRISTSKPGKINFTASMNHPGTVNIFTEGNDKLIMSG
                     ITSDCDSIRGEVKFQVQVKITTENGSIEASQNSLTVNNADVATIYISMASSFKNYSDI
                     SENAGEKAQSYLRNALRKNYKQALQNHIADYQRYFNRVNIDLGVTDSIKNPTDIRIEQ
                     FAKSNDPQLVALCFQFGRYLLISSSRPGTQPANLQGIWNNQLYPPWDSKYTVNINTEM
                     NYWLSEPTNLSEMNEPLVQMIEELSETGRQTAESMYGAKGWVLHHNTDIWRICGPVDG
                     AFWGMWPMGSAWLCQHLFEKYEYSGDREYLKSVYPAMRGAVEFYLDFLVEEPQYKWLV
                     VCPSVSPENAPSCHPEFSISAGTTMDNQLLFDLFTKTIKVAEILNTDKEFVIKIKETL
                     HQLPPMQIGRWGQLQEWMQDWDDPEDHHRHVSHLYGLYPSNQISPYRTPELFSAAKTS
                     LLARGDESTGWSMGWKVNLWARLLDGDHALKLITNQLSPSIQPDGSQKGGTYPNLFDA
                     HPPFQIDGNFGFTAGVVEMLVQSHDGFIFPLPALPEIWKNGNIAGIRLRGGFEIVKLE
                     WQNGRVSTLTIKSNIGGNCRIRSYDKLIPMGNFKLEEAKGENPNPFYTIPQVKEPLIS
                     PDANIIPVELRKSYRYDFNTKAGKEYTLILLE"
     gene            22273..23472
                     /locus_tag="PKX09_05380"
     CDS             22273..23472
                     /locus_tag="PKX09_05380"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOU17288.1"
                     /translation="MSNNGIFLRILSICSVLIILIQSSVISQVILDPDAVVEEIVSGI
                     QQPEGPVWREGLGLLFSDIKGNKIYKWTSEKGKEIYLDPSYNTNGLAYDLEGRLVAGQ
                     MGLRRVVRFEKDGSQTSLADSYDGKKLNSPNDLVIKSDGAIFFTDPDFNIPLGESKEL
                     SFNGVYRISPSGVLQLLTSSLTLPNGICFSPDESKLYVNDSQVHKIYVWDVVDDSTIT
                     NKKLLYTIPVPFSYADGMKIDVDGNIYCTCSSYVWVISPEGELIGKIALPSNVSASNC
                     AWGDDDNRTLFITAGNSVYRVRPFVTSINKEDSSLPNSFELSQNYPNPFNPTTKITYS
                     IPITSNVMITVYDVSGKEVVTIENCQKKAGTYTLLFNAEKLASGVYYYNLTANEFSST
                     KKFVILK"
     gene            23534..26317
                     /locus_tag="PKX09_05385"
     CDS             23534..26317
                     /locus_tag="PKX09_05385"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOU17289.1"
                     /translation="MEKLIRKSLCTALLLCLMSSTILYSQNITVNAGKPQIINWEVTH
                     KAQLEGSVSPSKIKVEWTCPQNPQVTFKDAANPVTEVTFPRPGYYLLVLSAKLKGKES
                     PSSSVIVNVFQPNSYKERLSDLIGLMTVDEKIKQLTNQSDPIPRLGVPRYNYWSEALH
                     GVLISGATSFPQVVALGATWDPELVRRVATAISDEARVFNVVEGKGLTYWSPTINIAR
                     DPRWGRNEESYSEDPFLLSRMGVAFVQGMQGNDPYYLKTVSTPKHFMANNEEERRHTG
                     SSDVDMRSMFEYYLPAFHQAVVEGRAYSIMGAYNELNHVPCNANMFLLTDLLRRNWGF
                     EGYVVTDCGAINDMLYGHHFFETGAEAVARSIRAGCDMSCGQEYREYLYGALDLGLLE
                     EKDLDSALTRVLSARFRLGEFDPPESVPYTSIPIEKLDCQEHRQLALESAQKAIVLLK
                     NDGILPLDRNKIKTIAVIGPNAAEAQLGIYSGFPNIRVSPLEGIKDKAAGQGIKVEYA
                     KGCDIGGGLLSVIEPQYFAKIAGTNKTGMKGEYFDNMELSGKPVVTRIDSTIDFSFGT
                     NSPAAGLPKDQFSVRWTGKIIPPDTIYHLGTSCDDGSRLYLNGKLLIDDWTDHGEKPI
                     GAEVKLIPGQEYEVVVEYYDNALGATARLTWDLNQVDFSSAKKVAAKNDATILVLGIS
                     PGISQEELDRKEIELPQVQRDLINEVASVNPNVVIVLVNGGPLALAGCENKAKAIVEA
                     WYGGEYGGKAIADVLFGDVNPGGKLPETFYASTQQLPPMSDYDLINHPRTYMYLNQPV
                     LFPFGHGLSYTQFEYSNLKVNSEAIKSDGVVEIQTTIKNTGKMKGDEVVQVYAHYMDA
                     TMTVPINQLKRFQRITLNPGESKVLTFKIPASEFSFYDTGINNLKTAKGKWELQVGSS
                     SKDIRLKEVFTIE"
     gene            26420..28342
                     /locus_tag="PKX09_05390"
     CDS             26420..28342
                     /locus_tag="PKX09_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020605397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOU17290.1"
                     /translation="MKQLFSPIVALCVFSMFVYAQSPVKYPPQGFDSLRVNIPHGKID
                     SVLYESKTIGTKRKAMIYTPPGYSKNKKYPVLYLLHGIGGDEKEWLNGGQPQVILDNL
                     YAENKIEPMIVVMPNGRAMKDDRAEGNIMEPEKVQAFETFEKDLLNDLIPFIEKKYSV
                     FKDREHRAIAGLSMGGGQALNFGLGNLDKFAWIGAFSAAPNTKAPEVLIPNPEEAKKK
                     LKLLWISCGDNDNLIMFSQRTHEYLQANDIPHIYYIMPGGHDFNVWKSSLYLFSQFLF
                     KPVDTSVFPKFTINGTPAPTNVRGAKYPQIFPDNSVKFRIKAPEAQKIQIDLGRKYDL
                     VKDSAGFWEVTTDPISEGFHYYSLLIDGVAVADPASETFYGMGRMASGIEMPIKGENY
                     YAVKDVPHGEIRIKRYFSTVFNKWRQFYIYTPPGYDENISEKYPVLYILHGGGEDERG
                     WANQGKADLILDNLIAQDKAMPMIIVMPDGNIESMGIFGEITLKMFEAELKNCIIPFV
                     EKNYRAKTDSRNRALAGLSMGGIQTLYAGINNSNMFSYLGVFSSGWIQPMQKDLADAQ
                     YNFMAKNADMLNSNIKLFWISMGGKEDIAYNNCQLMLAKFNEMKIKYVYSEYPGGHTW
                     PVWRNNLYNLAQLLFK"
     gene            28359..30314
                     /locus_tag="PKX09_05395"
     CDS             28359..30314
                     /locus_tag="PKX09_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HOU17291.1"
                     /translation="MSNTKFFYWILFILVISNNTLKAEIVTIKSPNQKITVSFWCTSA
                     GEPVYAVSYSGTIVLKQSQLGIIRFDEDFSKMLSVDSVSNESVVSDNYTLLSGKRSTC
                     HYLGHKRIFYLKNAHSKPMEIIFQVSDDGVAFRYHFPEKKDTFVKIFKELSSFHFELS
                     TKAFLQPCPDARTGWCFTQPSYEEYYQLELPVGTAAPYQAGWVMPALFKYENFWISIT
                     ETAVDTNYCGSRLSQFSPDGEYVIQFPQPQECCGNEPYLPESILPWYTPWRIIAVSEN
                     LAGIVESTLGTDLAIPAQYDVSSWLKPGIASWSWVILKDNATIFEIQKKYIDFAAEMK
                     WQYCLIDALWDQQIGYDKIIELADYAKAKNIKILLWYNSAGDWNTTNITPRDKLLKKE
                     SRAKEFQRLKEIGIGGIKVDFFGGDGQSMMKYYIGILNDAAKYNLAVNFHGCTYPRSW
                     HRTYPNLVSMEAIRGEEYVTFGQYFADNQPWHCTVIPFTRNLFDPMDFTPVNFSGIPN
                     IQRQTTKGFEIALSVLFTSGIQHMAETPEGMAIQDDFVKEYMSSLPQHWDDVKFIDGY
                     PGKFVVLARKTGEKWYIAGINGENTKRTLTLNIPFINKTAKGILITDAENSNELVKTN
                     ISLSRSFNVTINPNGGFVIVTLPSHNK"
     gene            30478..31824
                     /locus_tag="PKX09_05400"
     CDS             30478..31824
                     /locus_tag="PKX09_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOU17292.1"
                     /translation="MKNRKLYLFLVCLIATITNDVVAQNPIIRNQFTADPSARVFNDK
                     VYLYPSHDIPPENGRCRDNWFCMEDYHVFSSENLTEWTDHGIIVSQNKVPWVDSTSYS
                     MWAPDCIEKDGKYYFYFPARTKDTSFGRGFAIGVAIAEYPEGPFIPQPEPIRNVHGVD
                     PNVFIDKDGQAYLYWSMGNIYGAKLKENMIELASEPQMIKGLPEKGLKEGPYLFRRNG
                     IYYMTYPHVQTTIERLEYAMGTNPMGPFKYTGVIMDESPTGCWTNHHSIIEFKNQWYL
                     FYHHNDLSPQFDKNRSVRIDSLFFQEDGTIQKVSPTLRGVGMTAASQRIQIDRYSDIS
                     KEGASIAFNDSLNTFEGWKAMLDRKDAWIKYNSVDFGNKKFTSVIVKAYSEHGGTVQI
                     RLNSVAGPIVAEVKIPEGNKWNIITAPISKLKSGVQHLVVILKDKNQVEIDWISFE"
     gene            31858..32973
                     /locus_tag="PKX09_05405"
     CDS             31858..32973
                     /locus_tag="PKX09_05405"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOU17293.1"
                     /translation="MISFWNLLFFSTSLFAQVNPNNSTSYKGAATRSFCYTNPITRDT
                     AIAMRDHCIIKVGSKWYCTGTSMPFWTGPNPGVRLLVSDDLVNWRHHSWLIDASKLPP
                     ECPYNGRFWAPEIHYIQNKYWLTVNSGKVTEDDPKGMKTHSIWLFSSEEVTGPYKLVN
                     GPLTPQYNNDATLFEDNDGQTYLYCSGDGLFQAKIDLSTGKLIGNIQKFLDKRSPGNP
                     DWVIGGIEGPFVVKRDGIYFMFFSSWTRGYEVGLLKSKSPLGPWELASREPIFGTRKK
                     GYRYELALSGGYSDLKFNDSEDPYCETGHNALFEGPDGKLWSSCHYIMYDKRPYPYCP
                     TLKDWEQTPQLGIEPVYFVHGMFYINGPTWTEQCIEY"
     gene            33435..34973
                     /locus_tag="PKX09_05410"
     CDS             33435..34973
                     /locus_tag="PKX09_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOU17294.1"
                     /translation="MKKLFFLLLVLIPLSTFAQTATIKIDIDRAIGEIDPKIYGVFME
                     PIQFSGKRMGLPDTVGFNTMYGNLYDPASPLADENGFRKDYIEAMRELKITNMRWPGG
                     NYVMAYNWQDGIGPKNQRPARINLAWSGIDNNHVGTDEWIALNKSIGSENVICVNLGL
                     GTIQDACYWVEYCNFPKGTYYSDLRAKNGHSIPYNVKIWDLGNEVDGEPWELGHKNAD
                     DYVKIAREAAKAMRSVDKTIKLVASGSSYYESTGKWVEWNRKVLEGLGDMVDYISIHR
                     YWENSPDYYTYMGQSAVDFEEKIRVTAATIEAVKTMKNYTNPVYISFDEWGIMSRNFL
                     TVLPIAQSFNSFIRHADIVKMTNFTMLTSLLSTDKDKGSYKTPLFYTFKLFSNNCYGN
                     SIDTYVQCDTFNTEKYKGIPYLDVSTVYNNETKTVFINVVNRHKEKSIVTQIRSISGI
                     FTGKAEASVLNCESLDAPFTFDKQEQYKPIIKEIKVEENNISYSFPPHSFTQIKVAVK
                     KVAE"
     gene            35009..>35364
                     /locus_tag="PKX09_05415"
     CDS             35009..>35364
                     /locus_tag="PKX09_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU17295.1"
                     /translation="MKQISIFIFSLMICTNCFGQQYSKSWKDLDYGGDEKVYHKMDIY
                     LPQTEKPTYPVVVVIYGSAWFSNNLKETVLSSLGKPLLDAGFAIVTPNHRSSMDAKFP
                     AQINDIKAVIRFIRANA"
BASE COUNT        10619 a         7014 c         7843 g         9888 t
ORIGIN      
        1 ttaccattta gggctccaaa ttgatttgtt aaatgcatag gttttgtcaa atatctccct
       61 aaatatctgt tattacaata tattagatac cttttcagga aaccctaatt aatccccaat
      121 tattataatg tgttacctca acgaggcaca gatattttag aggagataat ttctatcctg
      181 tcaggatttt gtctgacctt attatcgcaa ggtaactatt gcggaattct ttcgattaat
      241 ccgaatttta tctaaattcg cgcgaacaaa tggaactagc tcttattaca acaaaaaaac
      301 ttcgtagata gtcgtgaaaa tagaaccgca attaaccact gactcgtaac taaaaaaatg
      361 ccttatctcg gttgtcacgt ctcgatcgaa aacagtttgg aaaaagcccc ggagcgcggc
      421 cgtgagctgg gctgtgacgc gattcagatt tttacttcca atcaaatgca gtggaaaggc
      481 gttccggttg cagacgaaac ggcggagaaa ttcctgaact atcaagccga acataagatt
      541 gcgatagtaa ttgctcacga ctcttatctg gtcaatctag caagtccgga gccggttaaa
      601 ttggcaatgt cgcgcaaggc ttttattgag gagattcggc gttcgcaagc actaaaaatt
      661 ccgtatatcg tttttcatcc cggttcgcat atgggtgccg gtgtggattt tgctttgcgt
      721 aaaatcgccg agagtctcga ttacgccatt gaaaaaacgc cggattgtcg tccgatgttt
      781 ctactggaaa cgactgctgg tcagggcacg aatgttggta acgctttcga ggaattgcgc
      841 cagattattg atttttctgc tcatccagaa cgactgggtg tttgtttcga cacctgtcat
      901 gcttatgcgg ctggttacga tttggttagt ccggcaaaat acgatgaaac ttttgccaga
      961 ttcgacgacg taatcggttt aagccaactg aaatgttttc acctaaacga tagcaaaaaa
     1021 ccgctcggat cgcgcgttga ccgccatgcc aatctcggag acggttttct gggctgggaa
     1081 gttttttatc gtttgataaa tgatgaccgt tttgggaatt tgccaatgat tctggagacg
     1141 cccggcggcg acgagaactt tgctaaagaa attgcctctc tgaaaaaggc attggagaaa
     1201 aaataataag gaataaccat ggataatttt gatttaatag tcattggatc aggaccgggc
     1261 ggatacgtag ccgctatccg cgccgcccag ctcgggatga aggtcgcaat agttgaaaaa
     1321 gccgaactcg gtggaatatg cctgaactgg ggctgtatcc cgaccaaagc cttgctcaaa
     1381 aatgcggaag tctggcagaa tctgcgcacg ttggacgaat tcgggatcac aatagctcaa
     1441 ccgcaactcg atttcaccca agttatccgc cgcagtcgtg aggtcgccgc tcgcctttcg
     1501 aaaggcatcg aatttctatt ccagaaaaat aaagtcacag tcgtcaaagg ttacggcaaa
     1561 ttgcgcgacc ggcaaacggt tgaggtcgtt aatccggacg ataattccac caccgggctc
     1621 aaggcccgaa atatcgtaat tgccaccggc gcccgcccgc gctcgattcc caatgtcgaa
     1681 attgacggaa aacgcattat cagtagtcgc gaagctatga ttttgccgca gattccgaaa
     1741 tccattatca tcatcggcgc aggcgccatt ggagttgaat ttgcctacct attcaacgcc
     1801 tttggtgtcg aagtaaatct ggttgaaata ctgccacaaa ttttaccggt cgaagatgag
     1861 gaaatcgcca gagaactgtc tcgcgcattt cgcaagaggc aaatcggcgt tcacattgac
     1921 accaaagtta aaagtgttca aacaaaaggc aatttcgtcg aagtggcgct cgcttccggc
     1981 ggcgaatcgc agattctggt cgccgactat gccttagtgg caaccggtgt tcagccgaat
     2041 tcagataatc tgggtctcgc agaactcgga gttagcactg aaaaaggctg gattaaagtt
     2101 gatgagcatt ttcggacgaa cataccggac gtttacgcca tcggcgatgt cattggcaat
     2161 ccttgcctgg cgcatgtcgc gtcggcggag ggcaagcacg cggttgaatt catagccggg
     2221 aagaatcccg cgccggtcaa ttattcggca attcctagct gcgtttactg tcagccacag
     2281 gttgccagcg tcggcttaac ggagaaaaga gcatcggaac ttggctacga attaaaaatc
     2341 gggcgtttta tgttccgcgc taacggcaag gcggtggctt ccggcgagag cgacggtttc
     2401 gtcaaagtca ttttcgacgg caagacgcaa aaattaatcg gtgcccacat catcggcacc
     2461 gaagcgactg aacttctacc ggaactagtt ctggcgattg atcatgggtt gacttatgac
     2521 gagctaaagg attctattca tgcccatccg acactatccg aggcgctcat cgaagccgtc
     2581 cacgacgctt tcggcgaatc ggttcaccag taataattaa gttaattaaa aaacgggata
     2641 gtcacgaact aacgtggtcg gaaattatcg aaaagaaaaa tactctggtg ccctctgcag
     2701 ttaaaatact agacgttctc gaccttggtc tgtgtcccta cggcgaagcc tgggaaatcc
     2761 agaaaaaatt acatgccgaa cgccttgcca gtcaaattcc ggatacgctg attcttgtcg
     2821 aacatcccaa tgtttatact ctggggaaaa atgccgatgc ccgacacttg ctcgcctcgc
     2881 aagaatattt gaaaaagcgc ggcatagaag tttatcaaat tgatcggggc ggcgacatta
     2941 cctatcacgg gccgggacaa ctggtcggct acccgatttt caatttaaaa gatcataaaa
     3001 cgagcattgc ttggtatgtc aattccgtcg aagaagtatt gattaaaact ctggtaaaat
     3061 tcggcatacg cgccgaacga atcaaaggct tgacgggaat ctggttggca aatcaaaaaa
     3121 ttgcggcgat cgggatgcgc gtctcgcact gggttacgat gcacggattt gcgctgaatg
     3181 tcacgacgga tttgtcgcta ttcggtggaa ttgtgccttg cggtctggcg acaaaaggcg
     3241 ttacccgaat gtgcgatttg aattcggcag ttactatgga tgctgtgaag gcggtcatca
     3301 taaaagagtt tgttgcccag tttggcttta gagaatcacg aatgaaaatg aattttaaca
     3361 ctagtaaacc cttataactg aaatcataga aaaagaattg gtctatagaa tcattgattg
     3421 tcccataaaa gttcataatg aatatattcc agaacttctt gtagaagata aaattattgt
     3481 ggaaatcaga actacctttg aatccaaaaa tatttcgtgt ttattaaaga tttattcata
     3541 ataattcctg gtgaaaaatg aaacctgatt ggctgaaagt aaaaattggt gtcaacgatg
     3601 atttccacga tttgcagaga atcattaata cccatcatct tcatactgtt tgtcaatcgg
     3661 cgcactgtcc caatcagagc gattgctggc agaggcgaac ggcgacacta atgattctcg
     3721 gaaatgtttg tacgcgtaat tgccggttct gcgccgttgc cagtgggaag ccaacggcaa
     3781 ttgatgagaa agaaccggct aaagttggtc aggcggtaaa cctgatgggt ttgaaatttg
     3841 ctgtgctgac atcggtgaca cgcgacgatt tgccggatgg cggcgcggca atctgggcga
     3901 aaacaatagg tgaaattcgt cgccaaaatc cggactgcaa aatcgaagtt ttgatacctg
     3961 attttcgggg taacatagac gctctcgaaa ttgtactaga cgccaaaccc gatatcctcg
     4021 gtcataatct cgaaaccgtt cgggcgctct atccgattgc gcgtccgcag gctgattatg
     4081 agcaatcgct tcggctcctt tccgaggcga aaaaacgtgg cgcagttacc aaaaccggca
     4141 ttatggtcgg attaggagaa tccgacgaac aaatccacga actaatgcaa gacgccttca
     4201 ccgccggttg cgatattctg acgatcggtc agtatcttca gccgacgaaa gatcaccttc
     4261 cggtcgcgcg ctatgttcat cctgaggaat tcaaatccta cgagacattc ggattgggaa
     4321 ttggttttcg ggtggttttt gctgggccgc tggtgcgtag ttcctttcac gccgccgaaa
     4381 ttgccggaac atagggttaa tccatctgtc aaactgctca caacttaaat gcttataata
     4441 aattaaaata cctacagcat ccaggtaatt ccaatcatac atccatacaa ttatgcaatc
     4501 cttttaataa tggattttct tactagaact taaaagcact tactaagcac ttatgaaatt
     4561 aatcgcaagt caagctattt ttaggattcg ttagatttgg cgctaatctt gccctttttt
     4621 actaaggttt catactgttc cgggtaaagt ttctgcatac aatctgggca aattccgtga
     4681 ctgaactcag ccgttgaatg ttggctaata taggtttcta catctttcca atagccggtg
     4741 tcgtcgcgga tttttttaca ggacgaacag atcggaagca agccagatag ggttctgact
     4801 tcggttagcg ctcggcgtaa gtcaacgacg gttttttcca aatgcatttc agctagtcgc
     4861 ttgtcggtaa cgtcgcgaaa atggaatata cgaccacata gtttttgctt ctttttgaaa
     4921 agcgatagga tgcgcagatc gtagatcttg gtttcattgc caaaggtaaa agaaatttct
     4981 tcctgaacgt tttttatttc agcaattaat tcgggccgaa tagctaatgg atttaggact
     5041 tcttcaaccg gttgcccgat tatgatttcg tttaacttta acatattccg acaagccgga
     5101 ttgtaatcgg ctacccggtt gcgcttgtct atgaccaaaa tagcgtcagt taagttctcc
     5161 agaatgcaat cacgggcgat tgggacaatt tccattattt gtaatcgaaa tagcccgaaa
     5221 gccattatag cccctgaaac tgatagtaaa gtcgaggtca tatcaagtcg cgaccagggt
     5281 gctggcaata gattaaaaac ataaagaaaa ttgccgaccc atggaaataa ggcgcaagcc
     5341 attaacgcca ggatttgcga acgacgcagt ttgggcggtt ggatcagacg ttgcatcagc
     5401 attatcgagc tggaaatcat caacaaattg ctataggcga aagtaaccca gaaccaaatg
     5461 ccataggatt tttttattat agaaaatgga ccgctggtgt ccagagtgat attccggcgc
     5521 atcaagccgt gataacgatt tgtccatatt agaaatacag tgataatcgg aattatacac
     5581 aataaaagaa ttgaccgacg ggtcgtaaac ttagtttgac cggtaaagtt ggctattaca
     5641 atgaaccagg ctacgataac catttcaatg ccgatatatt cgattgatac ccaaaacgtc
     5701 ttggtgctta gggtattaaa accgtattcc attatattgc aaaatgccca tataatacac
     5761 cctaaagcca aaaagaaaaa cgcaatacca tgactatcac ggcgaagccg ccacgaatgg
     5821 aacattatta ctgcacagat aataatggac aaaatcagag gcacataata aatgctaaag
     5881 gtaaaattca taagattctc gctgatttaa aaggttttgc ttccagggga atttgaaatg
     5941 cctatgcact tttaacgtgg ctcttaaaat cgctggagat attaaactgt tcaagttgat
     6001 aaaacttttt cgttattcca atttcgctta aagcgctagc tgaaccagat tggattagtt
     6061 agagcgaatt ttctgttttt agtttcaact tccgcccgaa aataaccgct gtttggtaga
     6121 tttgacactg ggaagtttat tatatttcta taaataccgc cagcaacttc tacggtctta
     6181 atttcttcca cttggcgctc aaaattaccg atgtacaatc ggatagaatg gatggctcca
     6241 caatgacgag ttgatagagc ggtgatttca acgctctgcg gtaattctgg catagtttgt
     6301 ccgatcgcat atcggttttg ccgcgaatcg atagccttaa tattcagaaa aggaccattg
     6361 gagacgatta cggctccgtt tttcaaattg gcgaccagat cgtcgagtcc ggtggcaaga
     6421 tcggtttgaa cgccagttaa gaactcacca aatatctggt gatcgttttc gtgcattaaa
     6481 agcagtggaa ttttcacttg tcgaaagcga ttgaagttgc catgggagtc attgccagca
     6541 tagatataac tatgattgcc ggaaagcaat ttttttatcc agaaattacg actggcgcta
     6601 aattcctttc cctcctgacc attcagaatt tgccaaccat tcaggcgttg gaactggtcg
     6661 tggagattcc aaataccgcg ccgggcaatg atttgttgag tgagaggcgg tttacagagc
     6721 gggtgagccg caaaaattaa cgcttgagat tcggctcgct cgagtacccg ctgataagtc
     6781 agttccgagg cgaaaccgac tgaacgctcc agtccatcac ccgagccggg gaaaaattca
     6841 gagttattta agactgccag gtggacattg cgtcccaagc cattatctac tgagacttct
     6901 tctccgggaa tgatgatgaa gtcattaagt ttggcattga tggcttgaac ggcttggcga
     6961 taagcgaacc attgacgtaa ttcgggatca ttttttgtcc aggaatccgg caaatcatcg
     7021 agatcataag aatgctcaac taacccacaa aatgatagtc ccatcgcttt cgccagacca
     7081 ggcagtacag ccaacggcac accgaactcg acctgatcgg aagtccattg tgaatgacaa
     7141 tgcaaatcgc cccaaatcca gccggaatca gctggcaatg gttccgaatc aacataaacg
     7201 ctgaaatccg cttgactgag accggcgtag ttatcatttt gaatagtaat aattcggccg
     7261 tcgcattcta aaattgctgt acaattaacg ctcaattgat gatttcggta tttggctacc
     7321 tccagactat attcgcgcgt aaaccacggt tcatctactt tgagattttc aataaaaact
     7381 tgctcaacgc ataagttatc gtcgaaagga gaggtaattt taaggataac ctgtttcaaa
     7441 gtaaccggaa accaatgagc gtctttgatt atcagaacga gtggcagtcg ggtagtttgt
     7501 acccgacgag gtacatcaaa gaggatttcc ggttgtcttt taaataacct cccccgtaga
     7561 aattttaacc ggtaatgcat ttcggcatac attattgccg gccagagcca gaaccaccaa
     7621 tccggtattg ctggaatcgt agtaaccgaa ttgaaattga caaacatagt ggtaaaatct
     7681 atgatttatt cgtattttaa tgaagaaaaa ttccgttgag aaaagtttga atagtctata
     7741 ttaattggaa aaatctttga aaataattca gcgtgcctgt aaattcctta tcactttaga
     7801 tttttaaaat ggagtacgct tggctacaac agcagacatc aagaatggct tggtgataaa
     7861 ttttaacggc gatctggtaa aggttattga atttcagcat attaaactgg gacgaggtgg
     7921 tgcgcgggtc tggaccaaat tcaaaaaaat ccgtaccgga caggttattg aaaacacctt
     7981 tcgctctggc gagaaaattg acattgtgcg tttagaagcc gtcgaaatgc agtttctata
     8041 tcgcgatggt tcagactatg ttttgatgaa taccgaaaca ttcgagcaga ttaccgtgcc
     8101 cgaggagata ttcaaagatg ccgcccaatt tatcaaggaa aacgaattgg tcaaggtgac
     8161 tttttttgat gggaaaccga ttgacgtcga agtgccgatt tttgttaatc tcaaggttgt
     8221 gaaaaccgaa ccgggcaaaa aaggtgacac agttaccggc gcctccaagc cagccgttct
     8281 tgaaacgggc tatacagtac aggttccact gtttatcaat gccggagata ttattcggat
     8341 tgatacgcgt agcggccagt actgcgaacg cgttaaataa aaacccggag gggtaatgag
     8401 gaaagaaaaa attctcgaat tagtgaagat tctcgaatcg cacgaaatcg acgaaatcga
     8461 aatttcgcgt tggggtcaga aaatccgcat tagtaaaaat agtaataccg gaatatctac
     8521 cggagtggaa gtagctccgg cgcctcaaaa agctaaaaaa ggtaataagc cacagttggc
     8581 ggaaacaatc acttttgaat ctcggacggc tgttacggaa gaaattaacc acaagggggt
     8641 tgaggttaaa gcgcctatag ttgggacttt ttatcgttca ccagcgccgg atgcggaacc
     8701 gtatgtaaat gtaggagata ccgtggctgt cgggcaaccg ctttgtatta ttgaagctat
     8761 gaaaataatg aatgtaatcg aagctgaggt cgccggaaaa gtgaccaaga ttctcgttga
     8821 aaatggccag ccggttgaat ataatcaaac cctttttata atcgaaaaaa ctgaataaca
     8881 tttctacgat tgaattttaa tgattaaaaa acttctgatt gccaatcggg gcgaaatagc
     8941 tttaagaata atccgggctt gtaaggaatt gaatattccg acggtggcag tttattcgac
     9001 cgcggacgct ctttctctgc acgtgcgatt tgccgatgaa gcagtttgca ttgggccgcc
     9061 tgaaagtaag aatagctatt taaaaatggc taacatcatc agtgccgcgg aagttactaa
     9121 ctccgatgcg attcatccgg gatatggttt tctggccgaa aatgccgatt tcgctcaaat
     9181 gtgcgaggat aatgagattc tatttgtcgg gccgcatccg gcggcaatcc ggacgatggg
     9241 caataaagct caggcacgcg agacggtcaa aaaggcgggg gtttcagtta ttccgggcac
     9301 agataccgtt gtgaaaaata tcgaggaagt caaacgcttt gccgaaagct acggatatcc
     9361 aattatcctg aaagccagcg ccggaggtgg aggccggggt atgcgcattg tccgccagat
     9421 tgccgaactc gaaaatgcct ttaataatgc tcgtgaagaa gctcagaaag ccttcggcaa
     9481 tcccgatata tacatcgaaa aattagtcga ggatgcccgc cacatcgaag tccaaataat
     9541 cggtgataat tacggtaatg tcattcatct gggtgaaaga gaatgctcga ttcagcgccg
     9601 ccatcaaaag ctgattgagg aatcgccttc gccggtggtt gatgaagaaa tgcgtgataa
     9661 actcggcaag attgcggtgc gagcggctaa agcggtcaaa tattccagtg ctggtaccat
     9721 tgaattttta gtggatagca agcgtaattt ttattttatg gaaatgaata ctcgtattca
     9781 agtggaacat ccggtaacgg aaatggtgac tggtattgat ctggtcaaag agcagatcag
     9841 gctggctgat ggcgctaaaa ttcccaaatg gatgaagaag ttcaacttgc gtggacatgc
     9901 gatcgaatgc cgcataaatg ccgaagaccc aaccaagaat tttaggccag cgccgggaaa
     9961 aattcaggct ttccattcac ccggcggtcc cggaacgcga atggatactc acgtttatgc
    10021 cggttacgaa attccttcct attatgattc tcttttaggt aaattaatta cgcatggaaa
    10081 agatcgggat gaggcaattg tcagaatgga acgcgcctta gatgaaacaa ttatagaagg
    10141 cgttcctacc accattcctt tccataaggc tataatcagg aatgaggctt ttcgaaaggg
    10201 cgattttaat attaattttt taaataattt ttcgttttaa taaggagaat atccgcatga
    10261 aagtaccagc caacttgaaa tatacgaaag atcatgaatg ggctagaatc gagggcgcgg
    10321 ttgcggtcgt cggtattacc gactacgctc aaagccaatt gggtgatgta gtttatattg
    10381 atttgccaaa agtcggcgat gtggtggcac gcggtgatgt aatcggaacg atcgaagcgg
    10441 ttaagactgt tgctgatatt tattcgccgg ttagtggtga aatcgttgcc gttaattcag
    10501 cgctaaagga tgcttccgaa ctcgtcaata aagatcctta cggcgaaggg tggatcgcca
    10561 gtattaaatt atccgattcg aacgaattag attctttact tgccccggcg gattacgaga
    10621 aattaatcag ttagattatg cacttcatac cgaatacctg cgatacggaa cgcgcaatct
    10681 tggagaacct gggagtcgcc agttttaacg acttgattgc caatattccg gaaaaattcc
    10741 gggagcaatg ccggatcaat ttaccgcccg ctctttcaga aattgagcta actaagacta
    10801 tgcaggaact gtctgagaaa aatggtaaca gtgataagca agtgtccttt ctcggcggtg
    10861 gctcttatga tcacttcatc ccggctgtgg ttgatttttt aatttcgcga tcggaatttt
    10921 atacagccta tactccttat caagctgaag tcagccaggg cacactccag gctatttatg
    10981 aataccagtc acaaatttgt catctgactg atatggaggt cgccaacgcc tcgatgtatg
    11041 atggtgtcac ggctttggca gaagcggcta ttatggcggc aaatattacc caacggaaaa
    11101 aatttctggt ttcgccgctg attaatccgc ttgctcgtca ggttctggaa acatatttga
    11161 ctgctcgtga tttcatcctt gccgacttgc cgggaaaaaa tggccaaaca gatttatccg
    11221 ttctgaaaga aatgattgat aatcagacgg cggccgtttt agtgcaatcg cccaattttt
    11281 ttggaattat tgaagattta aactatattg ccgaaattat tcatgctaaa agttctttat
    11341 taataatgtc cgttgatccg atttcattag ccatcctaaa aacacccggg gaactcggtg
    11401 cggatattgc cgtagccgaa ggtcagccgc tcgggattca tcaatcgttt ggtggtcctt
    11461 atctcggtgt tttcaccacg cgacggcaat atgttcgtaa tatgcccggc cgaatcgttg
    11521 gtcagacgat agatgtcgaa ggaaagcgcg gttatgtatt ggttttgcag acacgcgagc
    11581 aacatattcg gcgggaaaaa gccacttcga atatttgtac taaccagggt ttaatggcac
    11641 tagcggctac catctattta tctcttatgg gtaaaaccgg attgcggaaa gtagctgaac
    11701 tatgccttaa taatagccat tatctggcag ataaaattgc cggattgccc ggttattctt
    11761 tgccctttgc cggtcagccg ttttttaaag agtttgtcgt aaaaacgccc cgcccggcta
    11821 ccgaaatcgc ggcggcggcg cttgcaatcg gtttttttgc ggggattgat ctcggtcagt
    11881 atcgtcgtga atgggaaaat tatttgttga tcgccgtcac agaaaaacgt aatttgcaag
    11941 aaattagtga ttttgtaaaa attttggcta aattttaggt acaatgggag tcttaaatca
    12001 attactcgaa aaagcccgcc tgaaaggcgg tggatatttt gtattgattg atcctgacac
    12061 acgctcgacg ccggaattaa aagcgttcgt gcgagaaatt tgcgcctgtg gtgtggatgc
    12121 tattttagtc ggtggcagtt taattatcgg cggtggattc cacgaaaccc tggatattgt
    12181 caaaactgaa agcgacgtgc cagtgataat tttccccggt tcggtcgctc aggtttcgaa
    12241 ccgcgccgat gcggttttat acatttctct catcagcggg cgcaatccga acgctctatt
    12301 tggcgaacac gtaatcgccg caccagttat ccgcaaatta ggcattgaac cgatttctac
    12361 cggttatatg ctattcgaat ccggcaggac taccacggct gaatttatga gtaatacacg
    12421 tcctctgccg atcaataagc ctgaaattgc aatggcgcac gctttagccg ctcaatattt
    12481 gggaatgagt acagtttatc tggaagctgg tagcggagcc gagcgttcca tccccaatca
    12541 actcgtgaaa gccgtctgtg attatattga catcccggta tttgtaggcg gtggtattcg
    12601 tactccggac gaagcccgtg ccaaagtcga agccggggca acattcatcg taaccggcaa
    12661 tgtctttgat aatgacgcca atatcggatt ggctaaggag tttgtccgag ctgtgcatgt
    12721 caaagagcat ttttagaccg aagccatctt gcaccaaaaa ataatcgctc agttagaaga
    12781 aagcgctgaa gtaaagcgcc aaacgatcat aacggcggtt gactcgatcg aagccgccgc
    12841 ccaaatgtta attgattgct atcgcgccgg cggcaaggtg cttttatgtg gtaatggcgg
    12901 cagtgccgcg gacgcgcaac acttggcggc tgaattaatt tcgcgattga agttagaacg
    12961 agcggcgatt ccagccttag cgctgacgac caatacttct ctattgaccg ctatcgggaa
    13021 tgattacaaa tacgatttgg ttttcgtacg gcaagtggag gcttttggcc gagctggaga
    13081 tgtgctaatc gccatcagta ccagcggcga atctgaaaat gtgatcaaag ccgtggaatt
    13141 ttcccgcgtg cagggtatca aatccatagc gctgaccggt gcaaaaggcg ggcgtttagc
    13201 cgaaaaagtt gatcttgcga ttaaaatccc ttccgataac gttcagcgta ttcaggaatg
    13261 tcatattacc gtcggtcata ttctttgtga attaatcgag tcagctttat atggaactat
    13321 atgagccaat tgatcttaaa tgataagcga aattcaagat tttatcaatt atctcaaggt
    13381 cgaacgtaat ctcgccccga atactatcca agcctacagc actgatctgg aacgctatag
    13441 tcaatttcta acccaacaac aaattaacga cattagcaaa gtccgtttta gtgacatcca
    13501 aaattacatc aatgtattgg ctgaattggg attagcggcc agaagtctgg cgcgtaatta
    13561 cagctcgatt cgggctttcc atcgttttct atttagtgag cgcatcactg cgcaggatcc
    13621 gaccgaactc ttgcaatcac cgcgcttacc gcgccgacta ccgaaggtgc tcgaggttga
    13681 ggagattgac tccattttga atgccattga ttgtacggtc aataaaggca ttcgcgatcg
    13741 ggctttgatc gaaactctct attctaccgg cgtaagagtt tcagaattga ttggacttaa
    13801 aatgggcgat atattttttc accagggtgt tatgcgtgtt tttggtaagg gagccaaaga
    13861 acgcgtcgtg ccatttggaa accgcgcggc tcacgatatt aagaattata ttacggcagt
    13921 acggtcgttg ctaattagat acggtaaaag ccgcgatttt ctttttctaa gtatgcgcgg
    13981 ggctccgctc acccgaatgg cggtctggaa tattattcag gaatatgtac gtctggctga
    14041 tattcgcaaa ccgataagcc cccacgtttt ccgtcattca tttgcaactc atttattgga
    14101 aggaggcgct aatttgcgag cagtgcagga aatgctgggg cacgccgaca ttgctacgac
    14161 gcagatttac acccatctcg atcgcgaatt tttaatcgaa caacaccgca cttttcaccc
    14221 gaggtattaa tgaactttca agtaataatt ctgcttattc cgccgattct actggctctt
    14281 acctttcatg agtatatgca tggctggacg gcttacaaaa tgggcgatcc aacggcaaaa
    14341 atggctggac gtctgacaat gaacccaatt gcacatctcg atccgatcgg gacattgatg
    14401 ctttttctgg tacatttcgg ctgggcgcgt ccggtgccag ttgatccacg ctattttcgt
    14461 aatcccaaac gtgatatgct gatagtcgcc gccgccggac cgttagctaa tatgtttata
    14521 gctttgctga gcggtttatt ggcgagactt tttcagggcg gatattttag tttcctgccg
    14581 ctaagtgtgc tacagccgct ttatgtgatg gtaattctca gcttgcaaat caacctgggg
    14641 ctggcgattt tcaacctact gccaattcct ccgctggatg gttccaaaat cctgatggga
    14701 ctggttccac cgcgttatga agcccagttt aactggttag agcgatacgg cagttttatt
    14761 ttaatggctg ttataattgt cgggatggtt acgaatttct ctattctggg agctttcatt
    14821 aatccattcg taaacttttt ctcgcaatta tttggaggta tctaataatg tacacgagac
    14881 ccacaaaaaa atttgacata tctagcagac ttgaggaaca gcgtgacggg aaatttcatt
    14941 ttaaccgcca aagtagagtt cctcggaaat caccgcgctc tctgttatca actctgatat
    15001 ggatgatcat tatactattg gtgttgatgt ttttaaaaag gctattatta tagaattact
    15061 cgaaaaaatt ttctatatcg ttgtagtggc agggtaattg atgaccgaaa taatagaatg
    15121 cgtccccaat ttcagtgaag gtcgtgatca cgatgtaatc agggcaatcg cagtggcaat
    15181 cgaaaaggtg aaagacgtaa aactactgga tgtcaattcc ggtgctagtg tcaatcgcac
    15241 cgtgataacc tttgtgggca atcgcgaaag cgtcattaat ggggcttttg ctggaattgc
    15301 cagagccgcc gagctgattg atatgcgtca tcatcgcggc actcatccgc gactcggagc
    15361 taccgatgtt tgcccgctcg ttccaatttc cggaataagc gtggaagaat gtatcgctct
    15421 ctcgcatcaa ttggcaaaat gcgtcgctga ggaactatac atcccagttt atctttatga
    15481 aaaatctgct caaaaaccag aacgcattga tctggcaaat atccgcgccg gagaatacga
    15541 gggtctcaaa caaaaactca gcgatccgaa ctggcgaccc gattacgggc aaccaatttt
    15601 taatcccaag tccggggcga cgattatcgg cgttcgtaat ttcctgattg ccttcaatgt
    15661 taacattgat acccgcgacc gccaacaggc gaacgacatc gctctaacaa ttcgtgaaaa
    15721 aggtcgtatc aaacgcgatc aaaatggaaa taaaatgtat aattctcaag gtaaaccgat
    15781 ttacgaaact ggtttattga aaaatgtcaa ggcggttggc tggtacattg acgaatataa
    15841 aatggcgcag gtttcgacta atttgacgaa ctacaaagtt accccgccgc acgtcgtttt
    15901 cgaagaaatc cgacggctcg ccaatgccag aggtctgcgc gttacgggtt cggagctggt
    15961 cggactgatt ccaaaggagg cgcttctgat ggctgggcgt tattatctga aaaaacaagg
    16021 ttattcggaa gacgctccgg aaagtgagct catccagatg gcagtgcaat cgctcggttt
    16081 gaatgccatc gcgcctttcg atcccgccaa aaagattatc gaatatcgtt tggcggagta
    16141 tgaaaaacag ctcactagtt aaaatatctg taggctaccg atgaacccct gacttaagcg
    16201 accatcgatc cgccaggtca tccttcgaca agctcaggat gacaggtagg atgtcactct
    16261 gagcctgtcg aagagtgaca ggatcgctga ccctaagtct tgtgagggac tcatccttcg
    16321 acgagctcag gatgacaaag agcgacgagc tcaggatgac aaagagcgac gagctcatcc
    16381 ttcgacaagc tcaggatgac aggaaaagcg acaagctcag gatgacagga aaagcgacaa
    16441 gctcaggatg acaggaaaaa tgacaagctc aggatgacag ggcggctcct tgacattccg
    16501 tgaattccga ttaatcataa ataggcgtcc agcacagaaa aattaaaaga attggttaaa
    16561 ttactgaaac aataatgaat ggatttcaga attaatgtcg aaaataaaaa tactatcgga
    16621 aaatctgaca aataaaattg ccgctggaga agtcgtccag cgtccggctt cgattgtcaa
    16681 agaattagtc gaaaacagca tagacgccgg cgccgacgaa attatcgttg ttattcgcaa
    16741 cggtggtaaa acattatgcg aggtggttga taacggagag ggtatgagtc gcgatgatct
    16801 ccttctggct ttcgaacgat atgctaccag caaaattgcc accgtcgaag acctgaccaa
    16861 tatccacacc ctcggtttcc gcggcgaggc tctggcaagc attgcggcag tggcaatcgt
    16921 taccgccatc tctactctgc ggggcgcaaa tgaaggttat gaattgcgca tcgaaggcgg
    16981 cacgttccgc gacatcagac ccaccagtcc caaaccgggt acgacgattt cagtccgcaa
    17041 tttgttctat aatattcctg cccgtcgcaa atttctaaaa agtgccgagg ttgagtttcg
    17101 tcatatcgtc gatgttatgc aagaatttgc tctcatccat tacgatctgc gttttatttt
    17161 tatccacaat gatagagaaa tttacaactt gtcgccaagc agtctggcag aacgcatcgc
    17221 taatctcttt gccccagaat atagtcaaaa tcttctaccg gttgagtcgg tcgcagatga
    17281 tctaaaagtc accggctata tcgggaacct gaatctggtg cgcgcgcggc gtggtgaaca
    17341 atttttatac atcaatcgtc gctttgtgaa tgatcgcttg atgaatcatg cgataatcag
    17401 cggctatggc ggattgctgg cgcggagcga attcccattc tattgcctga acctagaact
    17461 gccgccggaa caaattgacg tcaatgtcca cccgactaaa atggaagtaa aatttcgtga
    17521 gcaagatcgc gtttatcagt ttttaaagca agccactgcg aacagcctga ggtcaattac
    17581 aaatgtcatt cctgatttag gaacttttgc gccgcaccat tattatacta cacctccgcc
    17641 accgaaagga acgcaaattc agatcccggc tccggatcaa tctccttcta aacagtcaga
    17701 atttgattca tcgagaaaaa ctttcgatgt tccagatgta gaaatcccgc ctacaccgtt
    17761 cgccgccggc gacagcacca agtggcagga acgggctcgg cgttttgtcg agcagggcga
    17821 gaccagcgag gctgatgatt ttcgtcccag tgtagcggtt tatcaagtcc ataataagta
    17881 tatcatcagt caggtcaagt ccgggctggt gattattgat cagcatgttg cccacgaacg
    17941 gatcctcttc gaagaagctc aggaatctat gcgcggtgaa aattggaaag cacaacagtt
    18001 gttgttcccg gtggttgtcg aactcaccgt gaatgatttt tccactctcc tagagatatt
    18061 accttttctg gaaaaactcg gattccgtat gcgggaattt ggcaaacaga cggttgccgt
    18121 tgaagcggtt ccggccggct tgcgctgggg caatgaaaat atcatcatcc gcgaaattct
    18181 cgatcactac caggaattcg gcaccaaaga caccagcatt caggataaag tcgccgccag
    18241 ttatgcttgt aaagccgcta tcaagtccgg cgatccgttg actacggagg aaatgcagaa
    18301 cctggtggat cgcttatttg cgaccagcaa tccttatttt tgtccacacg ggcgaccaat
    18361 tatcatcaat ctgtcgctga aagaattgga taaacgcttc gaaagggctt agcaattgag
    18421 cgctaattcc aacccgacta atgaaatacc ttctcgaaaa attcctttca ttatcgggcc
    18481 tacggcggta gggaaaactg cgactgcgat tgaactggca aagcagttga atggcgaaat
    18541 tatctcagtt gattcgcggc aagtatatag agggctggat attggcacgg ctaagccaac
    18601 tttgcgacaa cagcaagaaa ttccccacca tttagttgat attgtcgatc tcgatgagca
    18661 gattagcgct ggtgcttatc gcaaattagc tttaaaaacg gtaagcgaaa ttcgatcccg
    18721 cgggcgattg cccatttttg tgggcggttc cggcttatat gtcaatgccg tgctgaaagg
    18781 gattttcaat gaaagcacta ctaatcctga aatacgtcgg gaaatccggc aagaattaca
    18841 ggaaaaaggc gttgtggcgc tttataatca gctcctcgaa attgacccgg atacggtttt
    18901 gaaaattcat ataaacgatg tcaagcggat aacccgcgcc cttgaaattt accgcatcac
    18961 cggccagcca cccagttgtc acttccagaa gcaggatcgc cgaccgccat tcgcttaccg
    19021 aatttttatc ctgaatgctg atcgggagga gttatatcgt cgaatcaatg aacgagtcga
    19081 gcaaatgctt accgatggat tagtcgccga agtccagaaa ttaattgtgg ccggctatcg
    19141 gtcgaagctg gaatctctac gtacacttgg ctatcaggag gtcttaatct atctcgacga
    19201 aaagtgtagt tatgaagaaa tgcgcgaaaa tatcaagcag aatacgcgcc gctatgccaa
    19261 gcgtcaattg acctggtttc gtaaccaata tcccgaagcc gtttggatag acgttaccga
    19321 actctccaat ccgcttacca ttgcgaacac aatccggcaa aatctcaagc agacagaaat
    19381 tcaatcgcaa gcatagtact ttaatttcta ctgttgtaat taatcttttg ttttacgaat
    19441 agataaaata ttttaagagt tcagaaattg taatttcgat cctactctat tttcccgaaa
    19501 tccatatcaa ttacttggct gatatacaaa taagttatat attaattaaa gctgatttgg
    19561 tgttaattta aaataagata ttttttaaaa gattgagata aaaattaaaa gtagatttct
    19621 tgaaaacgtg atattcccaa aattttccat tttattggct ttcgctctga ttattagtag
    19681 ttgcgcaaaa aaacctgata gaaactcaga ttctgccaat aatccaatga aactctggta
    19741 tcaacagcca gccaaacaat gggtcgaagc gcttccaatt ggtaatggtc gtctgggtgc
    19801 catggttttt ggtgatccac ggcttgaaga aattcagctc aatgaaaaca ctatctgggg
    19861 tggtaagcca catcgcaatg ataatcctga agctaaagaa gcattgccca aaatccggca
    19921 attgattttt aatggtaaat ataaagaagc ccaggaacta gcggatcaaa aattcattag
    19981 taaaaattcg cagggtatgc cctatcaaac cgcaggaagc ctttaccttt cttttccgga
    20041 acacaaaaac tttacaaatt attatcgaga gctgaatatt gaaactgctg ttgttactac
    20101 ccgctatgaa gttgatggag taacgtataa gcgtgaagtt tttgcctcat ttcctgatca
    20161 ggttatcatt tgccgaatct caacgagcaa acccggtaaa atcaatttta ctgcttcaat
    20221 gaatcatcct ggcacagtaa atattttcac tgagggaaat gataaattga ttatgtctgg
    20281 aataacgagc gattgtgatt cgataagggg tgaagtaaaa tttcaagtgc aggtaaaaat
    20341 aactacagaa aatggctcga ttgaggcatc tcaaaacagc ctaactgtaa ataatgcaga
    20401 tgttgctaca atctatattt caatggcatc gagttttaaa aattacagcg atattagcga
    20461 aaatgctggc gagaaagcac agtcgtatct tcgaaacgcc ttgagaaaaa attataagca
    20521 agcgttacaa aatcatattg ctgattatca gcgctatttt aatagagtta atattgatct
    20581 cggagtgacc gattctataa aaaatccgac cgatattcgg attgaacaat ttgctaaaag
    20641 taacgatccg caattagttg cattgtgttt tcagtttggt cgttacttgc ttatttcttc
    20701 atcgcgccct ggtacacagc ctgctaatct gcagggaatt tggaacaacc agctctaccc
    20761 accctgggat agtaaatata cagtgaatat aaacacggag atgaactatt ggctttctga
    20821 gccaaccaat ttaagcgaaa tgaatgagcc gctagttcag atgatagaag aactctctga
    20881 aacaggcaga caaactgcgg aaagtatgta cggcgccaaa ggctgggtat tgcatcataa
    20941 cacggatatt tggcgtattt gcggaccggt agatggtgcg ttttggggaa tgtggccaat
    21001 gggcagtgcc tggctttgtc aacatttatt tgaaaaatat gaatatagcg gcgatagaga
    21061 atatcttaaa tctgtgtatc cagcaatgag aggcgctgtt gaattttatc ttgacttttt
    21121 agttgaagaa ccccaataca aatggttggt ggtctgtcca tcggtttctc cggaaaatgc
    21181 gccttcctgt catccggaat tttcaatttc agctgggaca actatggaca atcagctcct
    21241 ttttgaccta tttactaaaa caattaaggt ggcggaaatt ctcaacactg acaaagaatt
    21301 tgtcataaaa ataaaagaaa ccttgcatca attgccgcca atgcaaattg gcaggtgggg
    21361 gcagttgcaa gaatggatgc aggattggga tgatcctgaa gaccaccatc gccatgtttc
    21421 gcatttgtat ggcttatatc catccaatca aatatcgccc tatcgaacac cggaattatt
    21481 ttcagccgcc aaaacttctt tattagctcg cggcgatgaa tccaccggtt ggtcaatggg
    21541 gtggaaagtg aacttgtggg ctcgcctcct tgatggcgat catgctttga aactgattac
    21601 caatcaatta tctccatcaa tccaaccaga tggaagccaa aaaggcggga catatcccaa
    21661 tcttttcgat gctcatccac ctttccaaat tgatggaaat tttggattta cggcaggcgt
    21721 tgtggaaatg cttgtccaaa gtcatgatgg ttttatcttt ccattgccgg cattacctga
    21781 aatttggaaa aacggaaata ttgcaggtat tcgtttgcgc ggaggatttg aaattgtaaa
    21841 attagaatgg caaaatggca gggtttccac gttgactata aaatcaaaca taggcggtaa
    21901 ttgtagaata agaagctacg ataagcttat tcctatgggc aattttaaac ttgaagaagc
    21961 aaaaggggaa aatcccaacc cattttacac tattccgcaa gtaaaggaac ctttgatctc
    22021 tcctgatgca aatattattc ctgttgaact tcgtaaaagt tatcggtatg attttaatac
    22081 taaagctgga aaggagtaca ctttaatctt attagagtaa tgattaaaat ttatgaaaag
    22141 aatcttgccc gaaaagttta ccatccgatt agcgtttaga gaattaaaac tagtattaat
    22201 gatagattta ataaatgtat atctctggta gtatttatgt tagtccaatt ttaatgtaaa
    22261 agtgagagga aaatgagcaa taacggtatt tttttaagga ttctatctat ttgtagcgtt
    22321 ttgatcatat tgattcaatc gtcggttatt tctcaagtca tacttgatcc agatgccgta
    22381 gttgaagaga tagtttctgg aattcagcaa ccggaaggac cggtttggag agaaggtctc
    22441 ggtttattat tttctgatat taagggaaat aagatttata aatggacctc cgaaaaaggc
    22501 aaagagattt atttagatcc ttcttataac accaatggat tagcttatga tttggaaggt
    22561 cgccttgttg ccgggcagat ggggcttaga agagttgttc gttttgaaaa agatggctcg
    22621 caaacatcgc tggcagatag ttatgatgga aagaaattaa acagtccaaa tgatttggtt
    22681 attaaatctg atggcgcgat tttcttcacc gatccagatt ttaatatccc attgggagaa
    22741 agcaaggaac tcagtttcaa tggggtctat agaatcagtc cctctggtgt tcttcaactt
    22801 ctgacgtcgt cattgacttt gcctaatggt atctgctttt cccctgatga gtccaaactc
    22861 tatgtcaatg attctcaagt acataaaata tatgtttggg atgtggttga tgattctacc
    22921 ataaccaata aaaaactatt atatactatt cccgtaccat tcagttatgc ggacgggatg
    22981 aaaattgatg tagatggtaa tatttattgc acctgctcat cttatgtatg ggtaatttct
    23041 cctgagggcg aattaattgg gaaaattgct ttaccatcga atgtttccgc ttcaaattgt
    23101 gcctggggcg atgatgacaa taggacatta ttcattaccg ctggtaactc ggtttataga
    23161 gtgcgtccgt ttgttacttc aatcaataaa gaagactcga gtttacctaa tagttttgaa
    23221 ctttctcaga attatcctaa tccctttaat ccgactacaa aaattactta ttcaatccca
    23281 atcacttcaa atgtaatgat aacggtttat gatgtgtcgg gaaaagaagt agtgacgata
    23341 gagaattgtc agaaaaaagc cgggacatat actctattgt tcaatgctga aaaattagcg
    23401 agtggagtat attactacaa tcttaccgcg aatgaatttt catccaccaa aaaatttgtg
    23461 atattaaaat agttttaggt agtcatttga gattattaat taaaaaatat aaccaattaa
    23521 taggagcgta agcatggaaa aattaatcag aaagtcacta tgtacagcat tattattgtg
    23581 tttaatgagc agtactattt tgtactccca gaatatcaca gtaaatgccg gtaaacctca
    23641 aattatcaat tgggaagtaa ctcataaagc ccaactcgaa ggaagtgtgt ctcccagtaa
    23701 aattaaagta gaatggactt gtcctcaaaa tccgcaggtt actttcaaag acgcggccaa
    23761 tcccgttaca gaagttacct tccctcgtcc cggttattat ttattagttc tatcagctaa
    23821 actaaaaggc aaagaatcac caagcagttc agtaattgtc aatgtcttcc aaccaaattc
    23881 ttacaaagaa agactttccg atcttatcgg cttaatgacc gttgatgaaa agattaagca
    23941 gcttaccaat caatcggatc caattccgcg gttgggagtt ccccgataca actattggag
    24001 tgaagccctg catggagtcc ttatcagcgg tgcgacttct tttccacagg tagttgcatt
    24061 gggcgctacc tgggatcctg aacttgtccg tcgtgtcgct actgcaattt ccgacgaagc
    24121 gcgagttttt aatgttgtcg aggggaaagg tctaacgtac tggagtccga ctataaatat
    24181 tgcgcgcgat ccacgctggg gtcgcaacga agaatcctat agtgaagatc cttttcttct
    24241 ctctcggatg ggcgtggcgt ttgttcaagg tatgcaaggg aacgatcctt actatttgaa
    24301 aacggtttca acgcccaaac attttatggc aaacaatgaa gaagagcgcc gtcataccgg
    24361 ttcttccgat gttgatatgc gcagtatgtt cgaatattat ctaccggctt tccaccaagc
    24421 tgtggtagaa ggcagagctt attctattat gggcgcctat aatgaattga accatgttcc
    24481 gtgcaatgcg aatatgtttt tattgaccga tttgttgcga cgcaattggg ggttcgaggg
    24541 ctatgtcgta accgattgcg gcgccatcaa tgacatgctg tatgggcatc attttttcga
    24601 aacaggcgcc gaagccgtgg caaggagtat ccgcgccggt tgcgatatga gttgcggaca
    24661 ggaatatcgt gaatacctgt atggagctct cgatctcgga ttattggaag aaaaagacct
    24721 tgatagcgct ttaacgagag ttctctcggc gcgattcagg ttaggtgaat tcgatccacc
    24781 agaaagtgta ccatatactt cgattccaat agagaaactg gattgccagg agcatcgtca
    24841 gctggcactt gaatcggcgc agaaagcaat tgtgctactc aaaaacgatg gcatattacc
    24901 tctggataga aataaaataa aaacgatcgc cgttatcggt ccgaatgcgg cggaagctca
    24961 attgggtatt tacagtggat ttcctaatat tcgagtaagt ccgctcgaag gtataaaaga
    25021 taaagccgct gggcagggca ttaaagttga atatgctaag ggatgtgaca tcggtggtgg
    25081 actactaagc gtgatcgaac cacaatattt cgccaaaatc gccggaacaa ataaaaccgg
    25141 gatgaaaggc gagtattttg ataatatgga actatccggt aagccggttg taaccagaat
    25201 agattcgacg attgatttta gttttggcac taactcgcct gcggctggac ttcccaaaga
    25261 tcagttctcc gtgcggtgga ccggtaaaat cattccgccg gatacgattt atcatttggg
    25321 gacaagttgc gacgacggtt cacgccttta cctgaatggg aaattattaa ttgacgattg
    25381 gactgaccat ggcgaaaaac ctattggagc agaagtcaaa ctaataccgg gacaggaata
    25441 tgaagtagta gtagaatatt atgataatgc tcttggcgca acggcacggc tgacctggga
    25501 tctgaaccaa gtagatttta gttcggcgaa aaaggttgcg gcaaaaaatg atgctacgat
    25561 tcttgtactc ggaatttcac ccggaatttc tcaggaagaa ctcgatcgga aagaaatcga
    25621 attgcctcag gttcaaaggg atttaatcaa cgaagttgca tcggtcaatc caaatgttgt
    25681 catcgtcctt gttaatggtg ggcctcttgc tcttgctggt tgtgaaaaca aagcaaaagc
    25741 aatcgttgaa gcatggtatg gaggagagta cggcggcaaa gcaattgcag atgttctttt
    25801 tggcgatgta aatccaggcg gtaaacttcc tgaaactttc tatgcgtcaa cacagcaact
    25861 gccaccgatg tccgattatg atcttatcaa tcatccgcgc acgtatatgt atttgaatca
    25921 gcctgtcctt ttcccatttg ggcacggcct ttcctacaca caatttgaat acagcaatct
    25981 aaaggtaaac tccgaggcaa taaagagcga tggcgtggtg gaaattcaga caacgattaa
    26041 gaacactggt aaaatgaaag gcgatgaagt tgtccaggtc tatgcccatt atatggatgc
    26101 aacgatgaca gtaccgatca atcaattgaa acgattccaa agaattacac tgaatcccgg
    26161 cgagagtaaa gttctcacct tcaaaattcc agcctctgaa ttttcattct atgacacggg
    26221 aataaacaat ctgaagacag ccaaaggaaa gtgggaactt caagttggaa gttcatcaaa
    26281 agatatccgt ttgaaagagg tgtttacaat tgaatagaaa agctcttcta tgctgataat
    26341 agaattaata gataatctaa ctctctcggt aaaatgaaag catggaattt tataaattaa
    26401 caaattcaag ggtaatatta tgaaacagct attttccccc attgttgctt tatgtgtttt
    26461 tagtatgttt gtttatgcac aaagtcctgt taaatatcct cctcagggct ttgatagttt
    26521 acgagtaaat attccacatg gtaaaattga ttcggtcttg tatgaatcca aaacaattgg
    26581 cacaaaacgt aaagctatga tttatacacc tccaggatat tcaaagaata aaaaatatcc
    26641 tgttctgtat ttgttacatg gcatcggtgg tgatgaaaag gaatggctga atggcggtca
    26701 acctcaagta attctggata atctgtatgc agaaaataaa attgagccta tgattgtggt
    26761 tatgcccaat ggcagagcta tgaaagatga ccgagccgaa ggtaatatta tggaacctga
    26821 aaaagttcag gcttttgaaa cttttgagaa agatttgctc aacgatttaa tcccttttat
    26881 cgaaaaaaaa tattctgtat ttaaggatag agagcatagg gctatcgctg gactttcgat
    26941 gggtggagga caggcattga attttgggtt gggcaatctc gataaatttg catggattgg
    27001 agctttttca gcagcaccca atacaaaagc tccagaagtg ctgataccaa atcctgaaga
    27061 agccaaaaag aaattaaaat tactctggat atcctgtggc gacaatgata atcttataat
    27121 gttcagtcaa cgtacccatg agtatttaca ggcaaatgac attccccata tttactacat
    27181 tatgccaggt gggcacgatt tcaatgtatg gaaaagttcg ttgtaccttt tttctcaatt
    27241 tctttttaaa ccagttgata cttcggtgtt tcctaaattt actataaatg gtacaccagc
    27301 acctacgaat gttcgaggtg caaaatatcc acagatattc cccgataata gtgtgaaatt
    27361 cagaatcaaa gcacccgaag cacaaaagat tcaaattgat ctaggaagga aatatgacct
    27421 ggtgaaggac tctgcgggat tttgggaagt tacaacggat cccataagtg aaggttttca
    27481 ttactattcg ctattgattg atggtgttgc ggttgctgat cctgcaagtg agacttttta
    27541 tggaatggga cgaatggcaa gcggtattga aatgccaata aaaggagaaa attattatgc
    27601 tgtaaaagat gtaccgcacg gagaaataag aattaaacgg tacttttcaa cggtatttaa
    27661 taaatggaga caattttata tttacactcc acctggctat gacgaaaata tttctgaaaa
    27721 atatcctgtt ctctatattt tgcatggtgg aggtgaagat gagagaggct gggcaaatca
    27781 gggaaaagca gatttgatcc ttgataatct tattgctcag gacaaagcca tgccaatgat
    27841 tattgttatg cccgatggaa acattgagtc gatggggata tttggcgaaa taacattaaa
    27901 gatgtttgaa gcggaattga aaaattgtat cattccgttt gttgaaaaaa attaccgtgc
    27961 aaagactgat tccagaaatc gtgcacttgc aggcttatca atgggcggca tacagaccct
    28021 ttatgccgga atcaataatt caaatatgtt ctcatacctt ggagttttta gctctggctg
    28081 gatacaaccc atgcaaaagg atctggcaga cgcacaatat aattttatgg caaaaaatgc
    28141 tgatatgctc aacagcaata tcaaactttt ctggatttca atgggaggaa aggaggatat
    28201 agcatataat aattgtcagc tgatgcttgc aaaatttaat gagatgaaaa tcaaatatgt
    28261 ttacagcgaa tatccaggag gacatacctg gcccgtgtgg cggaacaatt tgtataactt
    28321 ggcacaattg ctctttaaat gattaaagat ataaaacgat gagtaataca aaattttttt
    28381 attggatcct atttatattg gttatttcta ataatacctt aaaagcggaa atagttacga
    28441 ttaaaagtcc aaatcagaaa attactgttt ctttttggtg cacttctgct ggagagccgg
    28501 tgtatgctgt ttcctattcg ggaacaattg tattgaaaca atcacaattg ggaattattc
    28561 gattcgatga agatttttcg aaaatgcttt ctgttgattc tgtatcgaac gaaagcgtcg
    28621 tttccgataa ctatactcta ttgtctggca agcgatctac atgtcactac ctgggtcata
    28681 agcgcatttt ttatttaaaa aatgcccact caaaacccat ggaaatcatt tttcaggttt
    28741 cggatgatgg ggtggcattt cgctaccatt ttccagaaaa aaaggacaca tttgtaaaaa
    28801 tatttaagga attgagttct tttcacttcg aactttccac aaaagcattt cttcaaccat
    28861 gtccagatgc ccgaaccggc tggtgcttta cacaaccatc ttacgaagaa tattatcagt
    28921 tagagttacc ggtaggaact gcagcacctt atcaggcagg ctgggttatg cctgcacttt
    28981 ttaaatacga aaatttctgg attagcatta cagaaactgc agtagatacc aattattgtg
    29041 gatcgcgttt aagtcaattt tcaccggatg gagaatatgt tattcaattt cctcaacccc
    29101 aggaatgctg tggtaacgaa ccttacctcc ccgaatcgat tctaccgtgg tacaccccct
    29161 ggcgtattat agccgttagc gaaaatcttg ctggaattgt cgaatctact ttgggtaccg
    29221 atttagccat ccctgctcaa tacgatgttt catcgtggtt aaaacctggt atagcttcat
    29281 ggagctgggt catactaaaa gataacgcta cgattttcga aattcagaaa aaatacattg
    29341 actttgcggc agagatgaaa tggcagtatt gtttaataga tgccttatgg gatcagcaaa
    29401 ttggttatga taaaataatt gaacttgctg attacgcaaa agcgaaaaat ataaaaattc
    29461 ttttatggta caattcagcc ggcgattgga atacaaccaa tataacacca cgcgacaaat
    29521 tgcttaagaa agaatcgaga gcaaaagaat ttcaacgttt aaaagaaata ggtattggcg
    29581 gaataaaagt ggactttttt ggtggtgatg gacaatcgat gatgaagtat tatataggta
    29641 ttttgaacga tgccgcaaaa tacaatcttg ctgttaattt tcatggttgc acctatccac
    29701 gaagctggca ccgtacctat cctaacctgg ttagcatgga ggccattcgg ggtgaagaat
    29761 atgttacgtt tggacagtat tttgccgaca atcaaccatg gcactgtaca gtgattcctt
    29821 ttacaagaaa tctttttgat ccgatggatt ttactccggt aaatttttcc ggcataccaa
    29881 acattcaacg ccaaacaacc aaaggttttg aaatagcctt gtctgtgctt tttacttcag
    29941 gaattcaaca catggccgaa acacccgaag gaatggctat tcaggatgat tttgtaaagg
    30001 aatatatgag cagtttacca caacattggg atgatgtgaa gtttatcgat ggttatcctg
    30061 gaaaatttgt tgtgcttgcg agaaagacag gtgaaaaatg gtacattgca ggtattaatg
    30121 gtgaaaatac aaaacgcaca ctgacattaa acattccttt tatcaataaa actgccaaag
    30181 ggattcttat tacagatgct gaaaattcaa atgaattggt aaaaaccaat atcagccttt
    30241 cacgttcttt taatgttaca ataaatccaa atggtggatt tgtaatagta actttaccgt
    30301 cacacaataa ataatcattg agattgtttc attaagattg ttgaagttta tatgaaaaca
    30361 ttacgaaaaa cagtaaccct tactatgata ataatatttt ctattcctat atttggtgaa
    30421 taagctgtaa ttagtagaag atgaaccaca aaacaaaaat ttataaatgg tcaatctatg
    30481 aaaaatcgaa agctatattt atttcttgtc tgcttaattg ctacaataac aaatgatgta
    30541 gtagcacaaa atcctattat tcgtaatcaa tttacagccg atccttctgc tcgggtcttc
    30601 aatgataaag tatatttata tccatcccat gatataccgc ctgaaaatgg gagatgtaga
    30661 gacaattggt tttgtatgga agattatcat gtgttttctt ctgagaatct tactgaatgg
    30721 actgatcatg ggattatcgt aagtcagaat aaagttcctt gggtggattc tacatcctac
    30781 agtatgtggg cgcctgattg tattgaaaaa gatgggaagt attacttcta ttttcctgct
    30841 cgaactaaag atacaagttt tggtcgtgga tttgctatcg gtgttgcaat tgcggaatac
    30901 ccagaaggtc cgtttatacc acaacccgaa ccaattagaa atgtccatgg tgttgatcct
    30961 aatgtgttta ttgataaaga tggtcaagca tatttgtatt ggtcaatggg aaatatttat
    31021 ggggcaaaat taaaagagaa catgattgag cttgcttctg aaccacaaat gataaaaggt
    31081 ctgccagaaa aaggacttaa ggaaggacct tatttattcc gaagaaatgg catttactat
    31141 atgacgtatc ctcacgttca gacaaccatc gaaagacttg aatatgcgat gggtaccaat
    31201 ccaatgggac cttttaagta tacaggagtg ataatggatg aatctcctac aggatgttgg
    31261 actaatcacc attcaattat cgagtttaaa aatcaatggt atcttttcta ccaccataat
    31321 gatttatccc cacaatttga taagaataga tccgttagaa ttgacagctt attctttcag
    31381 gaggatggta ctattcaaaa agtaagtcca acattacgag gagttggaat gactgctgca
    31441 tctcaaagga ttcagattga taggtatagt gatataagca aagaaggtgc atcaatagct
    31501 ttcaacgatt cccttaatac gttcgaaggg tggaaagcaa tgctcgatcg taaagacgcg
    31561 tggataaaat ataatagtgt tgattttgga aataagaaat ttacatcagt gatagtaaaa
    31621 gcttattcag aacatggcgg tactgtacaa ataagattga atagtgtagc gggtcccatt
    31681 gttgcggagg tgaaaattcc tgaagggaat aaatggaata ttattactgc tcctatatcc
    31741 aagctgaaat caggtgttca acatctcgtt gttatcttaa aagataagaa tcaagtcgaa
    31801 atagactgga ttagctttga atagcgttct taggagaata taattttgaa ttttaagttg
    31861 atctcatttt ggaatttatt atttttttca acttcccttt ttgcgcaggt aaatccaaat
    31921 aatagtacct catacaaagg cgctgctaca cgaagttttt gttataccaa cccgataacg
    31981 agagatactg ctatagctat gcgggatcat tgcattatta aggtaggaag taaatggtat
    32041 tgtactggaa cctcgatgcc attttggact ggaccaaatc ctggtgtaag attattggta
    32101 tcagatgatc ttgtaaattg gagacaccat tcgtggctta ttgatgctag taaactgccc
    32161 ccggaatgtc cttataacgg acgattctgg gcgcccgaga ttcactacat tcaaaataaa
    32221 tactggctaa cggtgaacag cggaaaagtt accgaagatg atccgaaggg aatgaagacc
    32281 catagtatat ggttgttttc atctgaagaa gttacaggac cttataaact cgtcaacggt
    32341 cctttaacac ctcaatataa taacgatgca actttattcg aagataatga cgggcagact
    32401 tatctttact gcagtggaga cggacttttt caggcaaaaa tagatctatc gacaggtaag
    32461 cttattggaa acattcagaa gtttcttgat aaaagatcgc ctggtaatcc agattgggtg
    32521 atcggaggca tcgaaggtcc tttcgtagta aaaagggatg gcatttattt tatgtttttt
    32581 tcttcatgga ctcgtgggta tgaagttggt ttgctaaaat cgaaaagtcc gctcggacca
    32641 tgggaactag cttcgcgcga accgatcttc ggcactcgta aaaaaggtta tcgttatgaa
    32701 cttgccctta gcggcggtta ttcagatttg aaattcaatg acagtgaaga tccatattgt
    32761 gaaactggac ataatgcttt atttgaagga ccggatggca aattgtggag ttcttgtcat
    32821 tacattatgt acgataaacg tccttatccg tactgtccaa ctctcaaaga ttgggagcaa
    32881 acccctcaat tagggattga acctgtgtac tttgttcatg gaatgtttta tatcaatggt
    32941 cctacatgga ctgaacaatg tattgagtat taacccagtt tatacaataa agataacagc
    33001 aagaaaaaag gagaagcgaa atataaccaa atctgtggct tcaagatgga ccatcgctca
    33061 actcaacctc cgttaagact tcggttgccg agtagccgat aaggcgtatt gaggaacaga
    33121 ttatatttta ggtaaatccg accttagaaa aatatggtac gtaaaacgat gcaaacgaag
    33181 ggtcagcaca aaattctgtt tgaggctcgc gcttctaagc gagatgagtt gattttgtgc
    33241 agcgaagtga gccgatgttt tacccatatt tttcttcagt attgaacttt tggttctttt
    33301 cttttaagag aaaagaacaa gcgctttaat aaattgtgtt acttgttaga aatatctttt
    33361 tcttgcttct aattcataat ttagattaat aataatttgg atttaataaa taatcatcaa
    33421 caaggagtca aatcatgaaa aaattgtttt ttcttttgct cgtacttatt ccactatcaa
    33481 cattcgctca gacggcaaca attaaaattg atattgatag agcaatagga gaaatcgatc
    33541 ctaaaatcta tggtgtcttt atggaaccaa ttcaatttag tggtaagagg atgggattac
    33601 cagacactgt gggtttcaat actatgtatg gaaatttata tgatccagct tcacctctcg
    33661 cagatgagaa tggattcagg aaagattata tcgaggcaat gagagagttg aaaatcacta
    33721 atatgcgttg gcctggtgga aattacgtca tggcatacaa ctggcaggat ggcattggac
    33781 ctaagaatca gcgacctgct cgtatcaatc ttgcgtggag cgggatcgac aataatcatg
    33841 taggtacaga tgagtggata gctttgaata aatccattgg cagtgaaaat gtaatatgcg
    33901 taaatcttgg acttggtaca atacaagatg cttgctactg ggttgaatat tgtaattttc
    33961 caaaaggaac ctactactca gatttgcgtg caaaaaatgg acattccata ccttacaatg
    34021 ttaaaatatg ggatctcgga aacgaggtcg atggagaacc atgggaatta ggccataaaa
    34081 atgctgatga ttatgtaaag attgcaagag aagcagcaaa agcaatgaga tcggttgata
    34141 aaactatcaa attagttgct tctggatcgt cttattatga aagtacgggt aaatgggtag
    34201 aatggaatag aaaagtactt gaaggtctcg gcgatatggt agattatatt tcaattcatc
    34261 gctactggga aaattcgcca gattattata cctatatggg acaaagtgca gtagattttg
    34321 aagagaaaat acgcgtaacc gctgctacta tagaagcagt gaagactatg aaaaattata
    34381 caaatcctgt ttatatctct tttgatgaat ggggtataat gtctagaaat ttcttgacag
    34441 tacttccaat agcacaaagc ttcaactctt ttattcgcca tgccgatatt gttaaaatga
    34501 ccaattttac tatgcttacc tcactattga gtacggataa agataaagga agctataaaa
    34561 cccccttatt ttatacattc aaattgttct cgaataattg ctatggcaat tccatcgata
    34621 cgtatgtgca gtgcgatacc tttaatactg aaaagtataa aggcatccct tatctcgatg
    34681 tatcaaccgt gtacaataat gaaacaaaaa ctgtatttat taatgttgtg aaccgacaca
    34741 aagaaaaatc tattgtaact caaattagaa gcatatcagg catttttact gggaaagctg
    34801 aagctagtgt attaaactgt gagtcgctcg atgcgccttt cacgttcgat aagcaagagc
    34861 aatacaaacc aataataaaa gaaatcaaag tggaagagaa taatatttca tattcatttc
    34921 cgccccattc atttacacaa atcaaagtcg ctgtgaaaaa agttgcagag tagattcgat
    34981 ataaaaacat aataatagga gataaactat gaaacaaatt tcaattttta ttttttctct
    35041 aatgatctgt accaattgtt tcggtcagca atactcaaaa agctggaaag atctggatta
    35101 tggtggagat gaaaaggtat atcataaaat ggatatttat ttacctcaaa ctgaaaaacc
    35161 aacttatcca gttgtagttg taatttacgg tagcgcatgg tttagtaata acttaaaaga
    35221 aacagttctt tcctctttgg gcaaaccgct tcttgatgca ggttttgcta ttgtaacacc
    35281 caatcatcgt tcgagtatgg atgcaaaatt ccctgcacaa ataaatgata ttaaagctgt
    35341 tataagattt ataagagcta atgc
//
LOCUS       DAOSMP010000023        34652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_1274_length_34652_cov_17.500853, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000023 DAOSMP010000000
VERSION     DAOSMP010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 34652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34652
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1274_length_34652_cov_17.500853"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..459
                     /locus_tag="PKX09_05420"
     CDS             94..459
                     /locus_tag="PKX09_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17296.1"
                     /translation="MDRKSALVEPSRDLTGKNVCLYSAIFAEGKVQPVSAGNSKIQAS
                     NSNEAPQTKYQTLPITDRCGHWYLKFIWYLVLETWYLIPGIRTFVFANKQKKGVCYAL
                     QESRSPESMIAHFLRDEQS"
     gene            512..1159
                     /locus_tag="PKX09_05425"
     CDS             512..1159
                     /locus_tag="PKX09_05425"
                     /inference="COORDINATES: protein motif:HMM:NF022745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3103 family protein"
                     /protein_id="HOU17297.1"
                     /translation="MKREWSTDGESAILVAYTPLTTDDMEWEEIYAYDADLKEYILDA
                     KTEPDYPVIVVGINERSEINNQGSQGLLAKTTGITPFQFTLPHPRVANGEVVVQDIQI
                     EDNCEPWYKGDAEVIAKQNLYDGWTTTVDMGSYDEGTYYGKNILIAWEGTNPVSNPYV
                     KIYEDDYSSDDLMLNVATASITDEGWLNGPNAKITLKVGRNVLMDFSSFWFSIGD"
     gene            1223..3439
                     /locus_tag="PKX09_05430"
     CDS             1223..3439
                     /locus_tag="PKX09_05430"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU17298.1"
                     /translation="MRVLILCIILINTLLSQSISIHGLVCEKSTNEPIIGVNVFIEEQ
                     SIGCSTDENGYFTLVDLKAGKYEITFSHIQYEKHIVSIYLGERIEFLEVEMDKAEIKS
                     PEIEVYGNKYAQQYTTSHIRIDKNDIDYLPVIGEPDLFRTLESYPGVTTANDWNLGLY
                     VRGGNRDQNLILLDGMCIYSPYHLSGIFSTFDSDVIQQANMQKIICDASNGNRLSSVL
                     NINIRDGNSDVHSGYINISMLSSKMRLEGPLPFGSYMFSIRRTYADLLANAILTVANS
                     DWRFPYNFIDGTGKIVIKPWQRHRIELSSYISRDNYDVGTADQSDFPCYIWQNSAIGG
                     KHIYFISPKINLINQISYSSFKAEYLPDDTTYTEYIDNELSSIQYKSYFSYSGNSFGK
                     IRIGGEYQNIFYRLRSGGFSYQKLTIPRTEFEEISYFFDYSKKILSKIFFSIGSRYIV
                     AQKNKTGYSPFISLSYSINPSISTNIQFSRTHQNLLTLGTEEVLLSMFDAWVAIPEDI
                     PMMEADQGVIGLEYTGKLNLVLNLYKKNISKLIEYNTAKFSEIHPDFLSGTANSIGLE
                     LLFKKETEKYNASISYMYSKTRKKFNGESYKPKYDMPHYFTADIIYKYSEKATIGLKF
                     IYHSGANFTRQIGTYSTGFLDYEEGFISNNNAYSDGVIYSGRNEFRLPAYHRMDINYS
                     RKIKWYNKDFEWYINFINIYGRLNVLDYQDNEEKGETESYIQLPPMATIGIRGKLW"
     gene            3433..4365
                     /locus_tag="PKX09_05435"
     CDS             3433..4365
                     /locus_tag="PKX09_05435"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HOU17299.1"
                     /translation="MVKKFLCIMLIFAGFYCCEPTMPDYNIEKSKNMLVASSVLSPGN
                     PNQIFHISKTDLFESLPQEDLNEDSLKMIVNKNGNEYIAQPDSIIEWYGRFYYYLNLP
                     LKPHDRVTVNVIHPDYETIRAEVEIPDSVHCVIPVNDTLFYEMPEYLSFSWNEVASAE
                     AYFVKIYLSATNNDSNDFDIQLNNDYYHLLPLANVQTKCFYIISREEIIDCLKNVIQK
                     VNSYRYWDENEISLKLSDYESVCLYAKIFSLDENAIYIFEESNSQDELSGFNTKIELY
                     SNVENGRGVICAYSKTRSKNISFSKNLIEQCLND"
     gene            4358..6625
                     /locus_tag="PKX09_05440"
     CDS             4358..6625
                     /locus_tag="PKX09_05440"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU17300.1"
                     /translation="MINICKLLHYFKLCLAKKFAPTLFIIFYSLQYSTFIFAASISGS
                     VKVVESLEPLSGVNVFLANSNLGCSSDPNGYFAITGIEPGQYQLVASHIGYGVYQEFI
                     EINAGDRRFIDIRLQALPVLIDTVSVFGERRFRRELEGDLLNISSVELNLAPKLGEPD
                     LFRVIETFPGVTTVNDYNMGLHIRGGNSDQNLILLDGMPIYNPFHLIGIFSTFDESAI
                     QSANVSKSGIDPVYGNRLSSVIDVNVRDGTAAEHAGYINLSFLSSKVRAEGPLPFGSY
                     LLTVRRTYADLFVNTVTKIISSDIKLPYHFIDGMGKVVIKPGERNRLEITSYLGKDVY
                     DMRVIDGDDSSGVYNWQNKALGFNYSNVINPQMVFYLRGSMSQFGAEWVPEDTTKSEM
                     IDNVFTGKTINAYFRYDNSLFGQIKAGAEFCFNDYYLKTKGFSYQPIYFEKNKSLESS
                     AFISINRQFGALFNVGGGLRMTHFSIQDTLTLSPQLSLGWKITPSHEIQLHWGKYHQD
                     LMTIGTEEIILSMFDAWVTVPEDLPVMAAEQLAFSYRWQIHRNLALDLALYHKRFNHI
                     VEYNTTKYSPIDPDFVDGTGIANGLEILLKGNVGKMQGWLSYSYSLVEKTVNGVTYPP
                     KYDRPHDLDLVLNYQLTKKWSVGSRFVYHSGTPFTQIIGYMRQPLYEDFRTGSYPYEI
                     EVYGARNAWRYPDYHRLDINFVKQYSWRSHPMEFYIDLVNVYARLNVLAYAEHDDTWI
                     QMPPLATIGIRGKIW"
     gene            6619..7602
                     /locus_tag="PKX09_05445"
     CDS             6619..7602
                     /locus_tag="PKX09_05445"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HOU17301.1"
                     /translation="MVRTRFLLIIPALFLLWRCPAGPPTGPSTDISRELCANCLISPF
                     EDTTYVFVSRWSEISQYEFEDLVTPEMIINPYWDYWSISNATIRIEIDGKYLPVEEDS
                     VSGHNEFYKICHYYYYKFPNVDVHPGQHWELSVYHPDYDSLYAETVVPDSVEFINLPD
                     TIRDDSENLNFSWTDADGARGYLPLLSFTAINDTGKYWSVQIHADFTDEGDGVYSSSS
                     GYPRQNTSAEYKINTMLKEIKFCYDEDKYKIGDLSQFTSFYLKIYIYSLNEALYNIQR
                     WEVQPDELSGFNAPVRVFSNVEGGSGILGSFWRTASPKIIVDKEFLISHCQ"
     gene            complement(7796..9550)
                     /locus_tag="PKX09_05450"
     CDS             complement(7796..9550)
                     /locus_tag="PKX09_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17302.1"
                     /translation="MKALRLLLIVGWISIFLEAGMSDNPYQADSSRVETETQSVGIYN
                     IGDRIFERSSYIFLPYDDWGVKGVIQTFGLPVTALTMRNNGLLLTDPVYGQAPLTWLN
                     PRFDQIELEATAIKMTPRYFLRDKVFSRFDYYRGDYGFMNFSMLISGKLADTTTVWRF
                     VGENLGYDSYYGNLGAKLSQTGESLSQIYRLDLRAFWKSWQIDLNAAYQKYLPGYVKV
                     SPTTSSAYLSPSWNGNVKEYRAALGGIAIRASERDSTALGFQQNNFIYGQYVKDPRFG
                     FKGVADQSEFIFYYDRQISHFRLAMHFNPVTRSVTLRNGFYERQNLAAGGIGISSKKI
                     KSYWSLYLGAIDQYFDGEFHLRYHLSPKNQVGGRVASTYALYPLIYHLDFPVNQPISK
                     EGFQYRIGTIDFLHASRSLQLSLAFNQVNSNFYVPYKSAINDTTLIYLPQKLDAAYIT
                     ADGRLILPWRMTLTARGLISNNEEGVWLQGWGQIRQEIDLFQNNLRLYVAGELTYWDI
                     SKKLGWFEELRGIGQFGTEYFTNNRLDIVARIGARIEDFHIFYAIYNLEGRTFSTVPG
                     ANYRNRLQVFGVEWQFLD"
     gene            complement(9552..10112)
                     /locus_tag="PKX09_05455"
     CDS             complement(9552..10112)
                     /locus_tag="PKX09_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17303.1"
                     /translation="MNSRIRKITYSAIFIALIVGVGYALAFVPNVELVTALIFLSGVL
                     MGWKSGLIIGGLGEFLFSALNPLGSGLIFPPMLIAQVLAMVIIGGVGGLCRNFVLSWQ
                     PRLRNFIAIGLLGGLLTIFYDICVSIAYPLSAGFNWRGLVTTLAAGLAFSIIHIIVNL
                     IVFLFLVPVTAQRIARAIPYFREERR"
     gene            complement(10170..11144)
                     /locus_tag="PKX09_05460"
     CDS             complement(10170..11144)
                     /locus_tag="PKX09_05460"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOU17304.1"
                     /translation="MSKTFISDLKENDRVTSFFGVFSKAVRRTKENKIYLDLILVDRT
                     GKINAKIWDDVENLAEQFEAGDAVKVQGTVTIFNNELQIKVEKIRRVVPERDRAEGYD
                     AGDLILSTSKNIESMWEDLQQIIQSLKNPFLKAVVGAIYEKYGEQLKIHPGSMILHHA
                     FRGGLLEHIYTMCQVAEGVCQAYTELDRDLVMSGILLHDIGKLVELEAGMAVSYTDAG
                     YFLGHIVLGRDIVRDQCLAINGFPEILRLQLEHILLSHQGKHEFQSPREPQFLEALVV
                     YYIDELDTRFNQMKNDIATDLNEGKWTAKGGYFKRVLFKGERESDQNS"
     gene            11440..12885
                     /locus_tag="PKX09_05465"
     CDS             11440..12885
                     /locus_tag="PKX09_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU17305.1"
                     /translation="MIGIKNNDIQSDNPQSGRGFFPPIKLSWKLVLFTVILILFVTAA
                     ITYPIYWQTRQALENQLTNHLEQNIQLVREQIDTSLVNLLADYPELNSVRDSLETKLN
                     WQAGKFSAQAIYLIDASGRLLVIAGNEESALQSATLHAVEIASARNDQVTSSPLFADR
                     TGQYYKSVFTKITENNGQPVVLGLDAGADFLEITGKLRNQILSLTAIILIISIGLVLL
                     LARTMTKPLENLTKYAEAIGQGRSDLPVLNHRRDEIGFLSRTIQTMYGKISQREKENK
                     QFLASVAHEIRNPLGGMKINAELLLEDTDNLTNATKYATAIAREVDRLALIVDSFLAY
                     ARPIETNLIICDLNELLQQVISELQRDFPSAKITVSGQARVKVNPGKIRLCLLNLIRN
                     GLEAAPEIPIVNINITNKPGQTEVRFFNTGMPIAPEIQSQIFEAFFSTKPDGIGLGLA
                     ITKSLVEQHGGQIYLDHSNTSGTEFVLILPE"
     gene            12889..14265
                     /locus_tag="PKX09_05470"
     CDS             12889..14265
                     /locus_tag="PKX09_05470"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOU17306.1"
                     /translation="MNSPKSNYTILIVEDNQSMRDGLLASLQKEGYSVAGVADGQEAW
                     SLLQNQRFDLIITDIKMPGLDGLTLMKKVKNLAPMTNIILITAFPTVDIAVDAMKCGA
                     QDFITKPFALAELRQKVEALYRRKFVQLKPSLSEPEFPDLIGNSPAIRQIRQLIAKIA
                     PGDSPILITGESGVGKELVARLIHQASRQKNGPFLAVNCGALTETLLESELFGHEKGA
                     FTGAHKTHIGKFEQSNSGTLFLDEIGDMSTALQVKLLRVLQTKQFQRVGGEKFIASDF
                     RLICATNKNLEKKIKDGEFRSDLYYRINVIPLTIPPLRERPSDIPLLVEHLLRLKADR
                     LGRKIPRIEPSVMARLISYSWPGNVRELENFIERALVFIEEDIFSEKLFNFGDLPENT
                     SLLNQTDTPAARDLTAQISQIEREMIIKALRDQHGVKQRAAEQLRIKPSTLYYKLDKY
                     GIKPEEYE"
     gene            14258..15232
                     /locus_tag="PKX09_05475"
     CDS             14258..15232
                     /locus_tag="PKX09_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17307.1"
                     /translation="MNNNFTSRIAICGTLILILLLAKNIQSAPIDSLAYYQTQYNQQL
                     TKLRTLEKDYDKLLNDINAIDAQIIKINNASNLNWSDKRKLSRLTARKAEINAKMIPI
                     YTELTNQRTILNITFNRYYDFLSKSIDSTVNQYNTVGDSRQRQSLSRTLFELIDQRGQ
                     LLASQRQFSDQNEFPLPEKEDLVALISKYDFNEAIRRDISHILDAKIRQIDLMIAAAN
                     TEKELRQRLNQLNLEMNALSGESYPYQGGNFSDYENWDHATMSGKSAYRNRTDKAALP
                     ATTQSVTAADYLPLFQNIPTNDLSLYISQLDSLRRDYQKQLQEINGNK"
     gene            15244..16296
                     /locus_tag="PKX09_05480"
     CDS             15244..16296
                     /locus_tag="PKX09_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17308.1"
                     /translation="MLALYFRNLTLILFIVVLLLSGLLQADQKLSGYCKTEAGMETNI
                     LESDSTSQNSFTTPALANLHFYLSKRRLNFNVNATGGLLAYDYRPENKATLNISAQAN
                     YTVSRKFFLASTVNSFQKWWLEQDYAYHNTDWLIGGGLNLSHWNPSLSIILSQNLFRH
                     SSSLDNHRIGGLVEISSSLNPSLTFSAQSGYSQIDYLHRPLFSVENSVLDTTARQKDL
                     LFFGQIGLEHRRQALSGVRLKVITVVSNNDYSEYYGVGLNYYLSGKIGRVYCQVIADV
                     LLKKYRADLNQYFLYYNPDPEQNIQNQLLVGWELPLWKNLALTGRAAIIRNETHYSGI
                     YYNKWFVSGGLIYRKD"
     gene            16452..17690
                     /locus_tag="PKX09_05485"
     CDS             16452..17690
                     /locus_tag="PKX09_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOU17309.1"
                     /translation="MANYKIMILTLLSLLTLNRCPEKITGGDKPEPIRELSAIEKKVT
                     EADNNFGLKLFRQIISSETDKNIFISPLSISMALGMAYNGADGETRAEMHSILEFGDL
                     TAAEINQSYRNLIELLSSADPDVTFEIANSIWYRAGFSILSEFLQLNTTYFDAVVQAL
                     DFDRSDATDMINAWVNEKTHEKIPTIVDSPINPRTVMFLINAIYFKGTWTYEFDPERT
                     QDAPFYLKDGSQTTCEMMRHKATHGYYTNDQIQIADLTYGEIGFSMTIILPNAGVDVD
                     SIAAMLTTENWTEWTNSLTEAEINIYLPKFKTEYEIGLIPTLQALGMIQAFSSSEADF
                     DKINPNYDLYISDVKHKTFVDVNEEGTEAAAVTAITVGCTSAGPKEIYFIVNRPFIFV
                     LREKSTSTILFIGKIMNPVS"
     gene            17706..18965
                     /locus_tag="PKX09_05490"
     CDS             17706..18965
                     /locus_tag="PKX09_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOU17310.1"
                     /translation="MEKTTRNFLILIIFVAGLISCSDVTAPDSVEMPELPRSISKSEQ
                     TLIESSNAFGFDLFKILSNKANGNNLFVSPVSVSMALGMTANGAAGSTLEAMHSTLGF
                     ENLTEDEINQSYQSLINLLRSLDSKVKFRIANSIWYRNEFTALPTFIDVNQTYFDAVV
                     RGLDFNQDAAVDTMNAWIEAQTDGFIKQLIEKPINTNTIMFLLNAIYFKGTWTYQFNE
                     EHTQEAPFYLNNDNTANCQMMSLSSDFDYAETDELQIIDLPYNNGFFTMTVLLPQSSD
                     DLNSLISGMTDEKWNNLIAGLSTTTVSLYLPKFKLEWFQVLNDQLSELGMGIAFDNRA
                     DFSRINDQYGSELYISEVRHKSFIEVDEEGTEAAAAASVEITYKSVSDDTEDITVMRV
                     DHPFIFAIRERASGTILFIGKIINPVS"
     gene            18981..19334
                     /locus_tag="PKX09_05495"
     CDS             18981..19334
                     /locus_tag="PKX09_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17311.1"
                     /translation="MKYFAIFLILILLFYCSCKYSTSPNENPDWVENLITKFQNEPVG
                     NPPQSIWQYKYKGQIVYYIPAQCCDQFSTLYNAEGDIICAPDGGFSGRGDGECSDFFQ
                     ERKNEKLIWQDSRTR"
     gene            19367..21154
                     /locus_tag="PKX09_05500"
     CDS             19367..21154
                     /locus_tag="PKX09_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17312.1"
                     /translation="MKTYKLFFSLNIFLMLLNITFATVPDPVTRQPAIPYLMEPIGFR
                     TMQMAIFAPEVSGMVPDKFSDLSWNPAYILREKQKTFYIDFNLENSVTSSLNPLSSGI
                     LTDSYYLAPSWYSQTSVQDINIAPLYNLALILPLNKRMSLGIVNRSVFDYGPYRNSIW
                     WTSGGWFDKAIESNSNIRYADFEPQRLETDENQQTVIANQSEITLGYRLSTKTDLGLH
                     LGHYIFHRDGYLLDSQWATHPHSSYGILNDEDLEINGCHYDLGAGFLYHPDEKTTYGI
                     SVGAFFGSGQESEIAQDTSDSFSETATNTAYYCLSEHTLNTDLTYKTSGVKPYLTLIL
                     EKKLNKNLVLRSSFSLTSKNQDVNTRYATDDTTFSDRTYNHWITDTTYAFRRYTANSA
                     AEFNLNGSGEESINQWKFMTALIYSLENDWAFFGGVQISRKWSQVDFDERSNYYSNYF
                     TEYSLYNASTYRNLDTYTKKYSYSQETKAWNLSVPIGVKAYLAKGFSVLLGTDINLTL
                     YKENSYSDLLYPNIVSKYWINDALANDDPETNRYERYDSNPPKTLTRTNAVRFGMVYE
                     HSSGAKLFIRSDGDIMNTNGWTLGFEMNW"
     gene            21212..23674
                     /locus_tag="PKX09_05505"
     CDS             21212..23674
                     /locus_tag="PKX09_05505"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HOU17313.1"
                     /translation="MKRFSELFKLALCFSFCFLGLQAQSQMIANISEDVQTDFGIYQP
                     YTANFTPDVPLFSVEPDFSNVENFSDFYGFSAVDSALLLQNHFTVRRSQFKQLYDIYN
                     DCTWDGTPLFVTTDAVLHIYHVLYDKILAEIEIQKFVPALELLTKTLIDSTQSQYDTA
                     TEPEIKETLRRNLAFLCVSQKLLKGNDFTVPEPVSALVDSELTLIANHDGFYTPPVLG
                     PFNLLDYSQFIPRGHYTTNDTLTAYFKAMMWQGWTIFTMEPAKFDNLARRHTLQALLL
                     TQMLFNLDANGNSLLDLWKMIYEPTVFFVGKTDDPNILHYKTIAAQVYGSDFLSLSAD
                     SLANSTLLENFMTEAQKLPEPKIPNWIYGSFTTYKGFRLMGQRFIPDSYMFAHLVLPE
                     VGGRAFPKGLDIMAILGSDRAYDLLDSLYNETAYPNYPEKITEFKTEFAAISPAEWAQ
                     NLYWNWLYCLMPLLYEKGEGYPFFMQTIAWMDKELLTALASWAELRHDTILYAKQSMS
                     PCGIAPGPPKSYVEPNPHLYARLASLTRYTIDGLQARDLLTEKFRDKLALFESLLLFL
                     QDVSIKELENTPLTDEEYANIYCFGKVMQKLVSEGEDPWHYDSDDMAVVADVHTDSDS
                     DQCLEEGVGYPLEINVIVNEGGTIRITRGAIFSYYEFIQPIANRLTDEAWREMLASET
                     PPTLPQWYDGLMDMNASQPSFMENSPDNLYHGEFQAIEKPDDSQKPKTFQVRQNFPNP
                     FNPLTTIEYELPQYSHVRLEIYNLLGQKITTLADNHQPAGRYHLIWNGRDSHGNNVAS
                     GIYFYKLTAGIFTQVKKMVLTR"
     gene            23793..24671
                     /gene="mtgA"
                     /locus_tag="PKX09_05510"
     CDS             23793..24671
                     /gene="mtgA"
                     /locus_tag="PKX09_05510"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOU17314.1"
                     /translation="MLKLILKIVLFIILIPVVIFVGLYIAAPDGSEFVHKNPETTAFI
                     RQRTAEYQKAGKKVALHWQWVPITKISPNIIHAVILAEDARFYEHIGFDVEAIKFALE
                     KNKKRKKYAFGGSTITQQLAKNLYLSTKKSIPRKVQELIIAFKMERHLSKRRILELYL
                     NVIEFGRGIYGVEAACQHYFGKSAADVSIDEASRLVAIMPSPRRHSPYDGSNFTERRR
                     KWLLNWLYKTGHIDSLDYVILYEGRADNLLEVVDSTGIDQFMQEAIDDSLKSDLEYIS
                     RYLPLDNKIQVSDSIK"
     gene            24868..25005
                     /locus_tag="PKX09_05515"
     CDS             24868..25005
                     /locus_tag="PKX09_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17315.1"
                     /translation="MKKLLVILMVASLALVTVSCQKKTKVEEAPAPVDTTTAVTDTVN
                     A"
     gene            complement(25259..25846)
                     /locus_tag="PKX09_05520"
     CDS             complement(25259..25846)
                     /locus_tag="PKX09_05520"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOU17316.1"
                     /translation="MIYQTVILAIVAYFLGSISFSYIFTKVRTGKDIRTVGVKNSGAL
                     NVFENVGKGIGLLAGIADALKAVVVVVIGKLIGLDTFPTIFAASFAVIGHCFPIYYKF
                     YGGRGASAALGILLCFIPLEVLISCIPAALIAYCIRRMGFTPVFILGFSPIVAAIFKK
                     PATIIWGVVYLVLLTGVLNLIINISKRDEKLIPEN"
     gene            complement(25859..26740)
                     /locus_tag="PKX09_05525"
     CDS             complement(25859..26740)
                     /locus_tag="PKX09_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOU17317.1"
                     /translation="MDQKILILTGDNLGLKNEQIDYPDVAVVKFPVVIDEKEYFEDET
                     HTAQYLIERFKKEKIVARSQALLRKDIVEVIEKNKDKYDLIVHVLMGSNMSSATVQMA
                     EGIRKDYEGIIPIINIDTKQASSGVGAVLLRVIELLKESDSVDEFVSRIDEVIKNTYS
                     YFCVPDLNYLYRGGRIGRAQSLLGSVLRIIPVVGLFGDETDSGFLPVGKGRTYQAANK
                     IIINHIKQKMVLKKVAKIELINILHFENSDADELKDLEKQINDNLTYRRLVHGHARFV
                     EAVHGGPGTWGGSFVLK"
     gene            complement(26752..28551)
                     /locus_tag="PKX09_05530"
     CDS             complement(26752..28551)
                     /locus_tag="PKX09_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOU17318.1"
                     /translation="MEITASVLNSILKDALTVLSIEKNRINDLNVFPVPDGDTGLNMV
                     LTVQGGLAHAKNYASDQSTLSEYLTALAEGMTLNSRGCSGVILALFIQGVAKKLADWP
                     PDQKIPNTEIAQALISGYKYAYAETANPKEGTMLTMMRVFAERFSELVMDGKDALSII
                     KDIVPDLEETLEKTPEMLPVLKKAGVVDSGAMGFLVLIKGVATGLEYTGTRIRPIMNI
                     ATILSTSRYIRSIIKERKYESGEERKKLRPLNISLNNINNISFANILTKFNNLLQRNN
                     NHQLAEEILAQSNELDETWNPDIQYRYCTEFILESDEIGKDSLKKKLESFGDSILIIS
                     SSNTYKCHIHTNKPKDLLKSLENLGNISATKIDDMKVQHKNLISEDKEFYERDLAVLL
                     IVNGEGFAKILKSLGATDILMYKQAKPSVNQIGNALFKTRAKNIIVAADDSDILASLK
                     SAITLCKSNIELIETQSVIQIISMMYTFSPEMDIFSNASIMRENLDNIRFCQIAKATR
                     QFKTDFDTIPKGNYFCLHNKSILAHNSNLIDLIKLSIEKVRENESLITLYRGKMEKNN
                     GSLIAELQQKFTQCEFEIYYGGQNRYNYYITFE"
     gene            complement(29156..30430)
                     /locus_tag="PKX09_05535"
     CDS             complement(29156..30430)
                     /locus_tag="PKX09_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17319.1"
                     /translation="MKRAIFWLVILLIWFGNTAVAQITLRGFILNQDMFQTNESMDVM
                     LIRNRLRLNTETYGENVYGFASLDITNDIADSTTTLPNLREVYIDIYSNWLDLRIGKQ
                     QVVWGKTDGYFINDIVNPLDLSYFLLQDFDDIRMATTMLNAKLHYSNYSLEVLVIPEF
                     KPMNLLFEGAWGYKRPQMMEYESPLGKIVLPLHYQNEQIPDLSLKNTEYGLKLNTWFL
                     RTDLAFLYLRSREDKPVYFTELVNDSSGLPIRVDLTPTHPWQNFFGLNFSKPIGAFVF
                     RGEGGYYPERHFNTQDMNYLSKGMIIERPFLQFMLGGDYQLTSALDLSVQGINERILD
                     YKEGIQADENVTIGTVMLRGHFVNETVSPLILVMYNFEDYSSLTRLSIDWNYADSFTI
                     TAGVDLLAGKSDTIFGMFAKNDNVYLKFKYAF"
     gene            complement(30427..31224)
                     /locus_tag="PKX09_05540"
     CDS             complement(30427..31224)
                     /locus_tag="PKX09_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009216858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOU17320.1"
                     /translation="MKKLFSLSIICIALVIFYSSAIAQMTGRDIMLKVEKQQDTKTRI
                     VKSKMILINKQNKTRERDVVQYVKSVNDPKGVDDKILIFFEYPADIRGTGLLMISYSD
                     VNKDDDRWLYLPALKKVRRIAGESKNEYFMGTDFTYDDMGGRSVDQDKHTLLGEETID
                     GVKCYKIESVPIDKNDIYSKKIGWVIPDKWVHLKVDFYDRKGQLMKTLKADDIRNVSG
                     VWLQFNLEMTNLAENHKTILKIIEAKINEPIDDQIFTITALERGGIR"
     gene            complement(31225..31581)
                     /locus_tag="PKX09_05545"
     CDS             complement(31225..31581)
                     /locus_tag="PKX09_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17321.1"
                     /translation="MTIEQKDKNIARGLTIISGGIVVYWLYQLILWLIRWFSTVRIET
                     SGIWSNFGGFCLENLSIILVVYVFICLQIAGVVEFRYRKNFTEAFFLGIIFTPIIMLI
                     YMKLKKGKFPDDSKEV"
     gene            complement(31578..33863)
                     /locus_tag="PKX09_05550"
     CDS             complement(31578..33863)
                     /locus_tag="PKX09_05550"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOU17322.1"
                     /translation="MIRKIIEFDLKYPRTIIAFCILLTLLMGWNIPKIKLEPDIKALM
                     PQDFEIIENMKDMENTFGGNDLVVVSITADNIFSPATLEKIEAITSEIEMLENVDQVI
                     SITNVPDIVGTADGFEVRELIEEFPTTESEIDSLKKRIIGNRMIYGTLASSDFQKAGI
                     IAYLKVATDENSDEQVYQIFNAIKNKYAGPEQIHIAGLPLTRREVSATMAADVKKLFP
                     YGIILMIFLLVLTFHSWTGAFLPFTVVIMSIVFTVGLMVLFNIKMTIVEMMIPVMLIA
                     IANSYSIQVITQYFVEHLKNPDADKDSLIRLILSYLTTPVLLSGFTTLVGFICLLSHV
                     LPPAKNLGILCACGTTIAFIYSLTFIPAALKLLGFPVTLKTASSRKRTSRFLPSWGQF
                     FTRYHKPFLGLCLVAIIIVATGIGRIIVDTNPIAYWKKSSEIRQSNEVIDRNFGGSST
                     ISVLATGDIKDPQFLKKIENLCTFLESQPTVTCANSIVDMLKRMNQAFHSDSAEYYVI
                     PETREEVAQYLFLYSLTGDTKDLDRFVDYDYTQAQILARVNDSSSKASYKLYKDTQNY
                     IAENLGAENFPKVSGMAPLIGVLSELVVDGQIRSLALSIIAVFLIIAIAFRSVKAGLI
                     AIVPLIVAALCLFGIMGHIGITLNIATALISSIMIGIGIDYTIHFLYRFRLEARNGTD
                     AEEATIRTLTTSGKGIVYNAISVIIGFMVLMLSGFEPISFFGFLIVFSIAVCLFGALT
                     ILPALLVLTKPAFIFGKRSLQ"
     gene            complement(33860..34489)
                     /locus_tag="PKX09_05555"
     CDS             complement(33860..34489)
                     /locus_tag="PKX09_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU17323.1"
                     /translation="MKQNSFPSGEEAKTKLSPAITYARRFILANGTRGLNIDQMAADL
                     HIAKKTIYKVFPTKEQFIEYVLLDIYHELFTETRAIQLNDNEPLKCFYGIMKIMFKHI
                     SQINPQTILDVKLFYPKVWQEVEEFEHEVINRILDSFQKADNLGLLRKKFDLKFTADL
                     IVRIVQNTFQPEIFISAPYSITEMIKIFVDIIMNGLMEKQFTFNFSDDE"
BASE COUNT        10328 a         7508 c         6905 g         9911 t
ORIGIN      
        1 gcggttcttc aactgagtgt agttggtttt ttctcagagt ttttgattta ttcggcaatt
       61 taatgaatgt aaatttctat caagagggat agaatggatc gaaaatcagc cttagtggag
      121 cccagccgtg atttgactgg aaaaaatgtc tgtttatata gtgcaatctt tgcagaggga
      181 aaagtacaac ctgtttctgc aggaaattcc aaaatccaag catcaaattc caatgaagca
      241 ccacaaacca aatatcaaac tctcccaatc acggatcgtt gcggacattg gtatttgaaa
      301 tttatttggt atttggtgct tgaaacctgg tatttaattc ccggcattcg gacctttgta
      361 ttcgctaaca aacagaagaa aggagtatgc tatgcccttc aggaaagtag aagcccggag
      421 tcaatgattg cacattttct ccgggatgaa caatcgtaat ctcaacagag attacactca
      481 tttcgcatga ttttaataag atttttttga aattaaaaga gaatggagca cagatggaga
      541 atcggctatt ctggtagcct acactcctct aactactgat gatatggaat gggaagagat
      601 ttatgcctat gatgccgatc tgaaagaata tattctggat gccaagaccg agcctgatta
      661 tccggtaata gtggtgggga taaatgaaag aagtgaaatt aataatcaag gtagccaagg
      721 attattagca aaaaccaccg gtataacccc attccaattc acgttaccac atccacgggt
      781 tgcgaatggt gaagtcgtag tacaagatat acaaatcgaa gataattgtg aaccctggta
      841 taaaggagac gcagaagtta ttgccaaaca gaatctctat gatggctgga caactactgt
      901 agacatggga tcatatgatg aaggcactta ttatgggaaa aatatcctaa ttgcatggga
      961 aggtactaat cctgtcagta atccttatgt taaaatatat gaggatgatt actcatctga
     1021 tgatttaatg cttaatgtgg cgactgcatc aattactgat gaaggatggc ttaatggtcc
     1081 taatgcaaaa attactctta aagttggtcg taatgtttta atggattttt cttcattttg
     1141 gttttcgatt ggtgactaaa aatgtaggtg ggtattagta ttattgacta gtacccaccg
     1201 gctttctttt tagtgaggtc taatgagagt tttaatctta tgtataatat taatcaatac
     1261 tttattatct cagtctattt cgatccatgg tttggtctgt gaaaagtcta ccaatgaacc
     1321 aatcattggc gtaaatgttt ttatagagga acagagcatc ggttgctcga cagatgaaaa
     1381 cggctatttc acattagttg atttaaaagc aggcaagtat gaaattactt tttcccatat
     1441 tcaatatgaa aaacatatcg tatcaatcta cctcggagaa aggattgaat ttttagaagt
     1501 agaaatggac aaagccgaaa taaaaagccc tgaaattgaa gtgtatggta ataaatacgc
     1561 acaacaatat acgacaagtc atatcagaat cgacaaaaat gacatcgatt atctgccggt
     1621 aatcggtgag cctgatttat tccggacgct tgaatcgtat cctggagtga caaccgcaaa
     1681 cgactggaat ctcgggctat atgtaagagg tggaaatcgc gaccaaaacc tgattttact
     1741 ggatgggatg tgcatttata gtccctatca cctatcggga attttcagca cattcgattc
     1801 cgatgtcatt cagcaggcaa acatgcagaa aatcatctgc gacgcaagca atggaaaccg
     1861 gttaagcagt gttcttaata ttaatattcg cgatggcaat agtgatgtac atagcggcta
     1921 tattaatatc agtatgctca gttcaaaaat gagacttgaa ggtccactgc catttggaag
     1981 ttatatgttt agtattcgca gaacctacgc tgatctgctt gccaacgcaa ttctgacagt
     2041 tgcaaattcc gattggcggt ttccatacaa tttcatcgat ggaacgggta aaatcgtgat
     2101 aaaaccctgg caaagacatc gcatcgaatt atcatcctat attagtcgtg acaattacga
     2161 tgtcggcacc gccgatcaat cggattttcc atgctatatc tggcagaatt cggcaatcgg
     2221 tggaaaacat atctacttta tctcacccaa aataaaccta ataaaccaga tttcatacag
     2281 ctcgttcaaa gcagaatatc tacccgacga tacgacatat acggaataca tcgataatga
     2341 gttgagtagt attcaatata aatcctactt tagttattct ggcaattctt tcggaaaaat
     2401 cagaattggt ggtgaatatc aaaacatatt ttaccggttg agatcaggcg gatttagtta
     2461 tcagaaattg accattccac gaacagaatt tgaggaaatc tcctacttct tcgattacag
     2521 caaaaagata cttagtaaaa tcttcttttc tataggaagc aggtatattg ttgcacaaaa
     2581 aaataaaacg ggttactccc cattcattag tcttagttat tcgatcaatc cttcaatttc
     2641 aacgaatatt caattcagtc ggactcatca gaatttatta acacttggca ccgaagaagt
     2701 actgctttca atgttcgatg cctgggtggc aattccggaa gatataccta tgatggaagc
     2761 cgatcaggga gtaattggac ttgaatatac aggaaaatta aatctggttt taaatctata
     2821 taagaaaaat atcagtaagt tgatcgagta taacacggct aaattttccg aaatccatcc
     2881 agatttcctc agcggtacgg caaattcgat aggcctggag ctacttttta aaaaagaaac
     2941 tgaaaaatac aacgccagta tttcatatat gtattctaaa accaggaaaa agttcaatgg
     3001 tgaaagttac aaaccaaaat atgacatgcc acattacttt accgccgaca tcatctacaa
     3061 atattccgaa aaggcgacga ttggattaaa gttcatctat cattccggcg ccaattttac
     3121 aaggcaaatc ggcacatatt caacaggatt cctggattac gaagagggat ttattagtaa
     3181 caataatgcc tattcggacg gtgtcattta ctccgggcgt aatgaattcc gactaccagc
     3241 ctatcataga atggacatta attactccag gaaaatcaaa tggtacaata aagatttcga
     3301 atggtatatt aattttatca acatatatgg ccgtttaaat gttcttgatt atcaagacaa
     3361 tgaagagaaa ggagaaacgg aatcttatat ccagttgccg cccatggcga caatcggaat
     3421 tcgggggaaa ttatggtaaa gaaattcctg tgtattatgt tgatttttgc aggtttttac
     3481 tgttgtgaac cgacaatgcc tgattataat attgaaaaaa gcaagaatat gctggtcgcg
     3541 agttcggtac tatctcccgg taatccaaat cagatttttc acatttctaa aacggacctg
     3601 tttgaatcct tacctcagga ggatttgaac gaagattcat taaaaatgat agtcaataaa
     3661 aacggcaacg aatacattgc ccagcccgat agtattatag aatggtacgg acgcttttat
     3721 tattatttga acttaccttt aaaacctcac gatcgagtga ctgtcaatgt tattcatccg
     3781 gattatgaaa ctatacgggc ggaggtggaa attccggatt cggtgcattg cgtaattcca
     3841 gtaaatgata ccctgtttta cgaaatgccc gaatatttaa gtttttcatg gaatgaagtg
     3901 gcatcggccg aggcttattt tgtaaaaata tatctatctg caaccaacaa tgattccaac
     3961 gatttcgata ttcaattaaa taatgactat tatcacttat taccattggc aaatgtgcaa
     4021 actaaatgtt tctatattat atcaagagaa gagattattg attgccttaa aaatgtcatt
     4081 caaaaagtga atagttatcg ctactgggac gaaaatgaaa tcagcttaaa attatccgac
     4141 tatgaaagtg tctgcttata tgcaaaaata ttctccttgg atgaaaatgc tatctatatt
     4201 tttgaagaaa gtaatagcca ggatgaattg agtggtttca acacaaaaat tgaattatat
     4261 tcaaatgtag aaaatggccg aggcgtgata tgcgcatata gcaaaacaag aagtaaaaat
     4321 atttccttta gtaaaaatct aatcgagcag tgcttaaatg attaatattt gtaaattatt
     4381 acactatttc aaactatgtt tagccaagaa atttgcacca actttattca tcatatttta
     4441 ttctttacaa tattcaacct tcatctttgc cgccagcatt tccggctctg tcaaggttgt
     4501 cgaatcgctg gaaccattgt cgggtgtgaa tgttttcctg gcaaacagta acctgggatg
     4561 cagttctgac cccaacggtt attttgcgat taccgggatt gaaccgggcc aatatcaact
     4621 ggttgccagt cacattggtt atggagttta tcaggagttt attgaaatca atgccggaga
     4681 caggcgtttt attgatattc gtttacaggc tttgccggtc ttgattgata cggtttcggt
     4741 attcggcgag cggcgattca gacgcgaact cgaaggcgat ctgctgaata tcagttccgt
     4801 cgagttgaat ctcgccccga aactcggcga gcccgatcta tttcgggtca tcgagacttt
     4861 tcccggtgtg accaccgtga atgactacaa catgggatta catatccgcg gcggcaactc
     4921 cgaccagaat ctgatcctgc tggacggcat gccgatttat aatccctttc acctgatagg
     4981 aatattttcg acttttgacg aatcggcgat tcaatctgcc aatgtttcta aaagcggaat
     5041 cgatccggtc tacggcaatc gtctcagttc agttattgac gtcaatgtcc gggacggaac
     5101 cgccgccgaa catgccggct acatcaatct cagtttcctg agttccaaag tccgagctga
     5161 gggaccgttg ccattcggta gttatcttct gacggttcgc cggacttatg ccgatttgtt
     5221 cgtcaatact gttaccaaaa tcatatcgtc tgatattaaa ttgccgtatc atttcataga
     5281 cgggatggga aaagtcgtca ttaaacccgg agagcggaac cgcctggaaa tcacctccta
     5341 tcttggaaaa gacgtttatg atatgcgagt gatcgatgga gatgacagtt cgggggttta
     5401 taactggcag aataaggcgc ttggctttaa ttattccaat gtcatcaatc cgcaaatggt
     5461 tttttatctg cgcggcagta tgagccagtt cggggctgaa tgggttcccg aagatacaac
     5521 gaaatctgaa atgatcgata atgtctttac cggcaagacg attaacgctt acttccggta
     5581 tgacaattct ttatttggac agatcaaggc tggcgccgag ttttgtttca atgactatta
     5641 cctgaaaact aagggcttta gttatcagcc gatatatttc gaaaaaaata agtcactgga
     5701 gagttcggca tttatcagta tcaaccgcca gtttggggca ctgttcaatg ttggcggcgg
     5761 tctgcgaatg acgcattttt ccattcaaga tacgctgaca ctttcaccgc agttatctct
     5821 gggctggaaa atcactccat ctcacgaaat tcaattacat tggggaaaat atcatcagga
     5881 tttgatgacc atcggcacgg aagaaatcat cttgtcgatg ttcgacgcct gggtgacggt
     5941 tccagaagac ctgccggtta tggcggcgga acaactggct ttcagctatc ggtggcaaat
     6001 ccatcgtaat ctcgcgctgg atttggcatt atatcataag cgctttaatc atatagttga
     6061 atataatacc accaaatatt caccaattga cccggatttt gtggatggaa ccggcattgc
     6121 caatggtctg gagattctgc taaagggaaa tgtcgggaaa atgcagggct ggctgtcgta
     6181 ttcatattcg ctggttgaaa aaacagtcaa tggcgttact tatccgccta agtatgatcg
     6241 tccgcatgac ctcgacctgg ttttgaatta tcagctcacg aaaaaatggt cggtcggcag
     6301 tcgtttcgtc tatcattccg gcacgccttt tactcagata atcggatata tgcggcaacc
     6361 gctctatgag gatttccgga caggtagtta tccctatgaa atcgaagttt atggagcgcg
     6421 gaacgcctgg cgttatccgg actatcaccg gctggatatt aacttcgtca aacaatacag
     6481 ctggagatcg catccgatgg aattttatat tgatttggtc aatgtctatg cccgtctgaa
     6541 tgtcctggct tatgccgaac atgatgatac ctggattcag atgccgccgc tggcaacgat
     6601 cggaataagg gggaaaatat ggtaagaact agatttttat tgattatacc ggcattgttt
     6661 ttattatggc gctgtccagc cggaccaccg accgggccaa gtaccgatat cagccgggaa
     6721 ttatgtgcaa attgtcttat cagtccgttc gaagacacta cttacgtttt tgtctcgaga
     6781 tggagtgaaa taagtcagta cgagtttgaa gatctagtga ctccggaaat gataataaat
     6841 ccctattggg actattggag catttctaat gcaacaataa gaattgaaat tgatggaaaa
     6901 tatcttcctg ttgaagaaga ctcggttagc ggacataatg aattttataa aatctgtcat
     6961 tactactact ataaattccc gaatgtggat gtgcatccgg gacagcattg ggaattatca
     7021 gtctatcatc cggattacga cagcctctat gcagaaacgg ttgtgccgga ttctgtggag
     7081 tttatcaatt tgccggatac aattagggac gattcagaga acttaaactt ctcatggact
     7141 gatgcggatg gcgccagggg atatttacca ttattatctt ttacggcgat aaatgataca
     7201 ggtaaatatt ggagtgttca aatacacgca gattttactg atgaaggcga tggagtatac
     7261 tcatcttcct ccggttatcc ccgtcaaaat acatctgccg agtataaaat aaatactatg
     7321 ttaaaagaga ttaaattttg ttatgatgaa gataaatata aaattggtga tctgagccag
     7381 ttcacttctt tttatttgaa aatttacatt tattcattga acgaagccct ctataatatc
     7441 cagcgctggg aagttcagcc cgatgaactc agcggtttca atgccccagt gcgcgttttt
     7501 tccaatgtgg agggcggcag tggtattctc ggatcgtttt ggcgaacagc tagcccgaaa
     7561 atcatcgttg ataaagaatt tttaattagt cattgtcaat agaacatata ggtcaagtcg
     7621 ccaactcgac cattcgattt agcacgccgc tccataatgc aaaattccgt aggacaggca
     7681 tttttgcctg tctattcctg tctattttat ttacgaatta tattgccagg ctgacagcct
     7741 ggaatggccg tagccaggcc gacagcctag aaaggctatc ctacgaattc aaattttaat
     7801 ctaaaaactg ccactcaact ccaaaaactt gcaggcgatt ccgataatta gcgccaggaa
     7861 cagttgaaaa agtccgacct tccaaattat aaatggcata aaaaatatga aaatcttcga
     7921 tccgagcccc aattcgagcc actatatcca atcggttatt ggtaaaatat tccgttccga
     7981 attggcctat tcctcgcagt tcttcaaacc accccaactt tttcgatata tcccagtagg
     8041 tcaattctcc ggcgacgtac aaccgtaaat tattctggaa aaggtcaatt tcctgtcgga
     8101 tttgccccca gccttgcaac cagacgcctt cctcgttatt ggaaattaaa ccgcgcgccg
     8161 ttagcgtcat acgccatggc aagattaatc ttccatcggc ggttatgtaa gccgcatcca
     8221 atttctgggg cagataaatc aaagtcgtgt cattgattgc cgatttatag ggaacataaa
     8281 aattcgagtt cacttgatta aatgccaaac ttaattgtaa actccgactg gcgtgtagaa
     8341 agtcaatggt tccgatccgg tattggaagc cttccttgct aatcggctga ttcaccggaa
     8401 aatccaaatg gtaaattagc ggataaaggg cataagtcga ggcaacccgt cccccgactt
     8461 ggtttttcgg actcaggtga tatcggagat gaaattcgcc gtcgaaatat tgatcgatcg
     8521 cacccaagta aagactccaa taacttttaa tttttttcga tgaaatgcca atgccaccag
     8581 ccgctaaatt ttgacgctcg tagaagccat tacggagagt tacagagcga gttacgggat
     8641 taaagtgcat cgctaagcgg aagtgggaaa tctgccggtc gtaataaaat ataaattccg
     8701 attgatcagc cacgcctttg aatccaaatc gaggatcttt gacatattgt ccgtaaataa
     8761 aattattttg ctgaaaacca agcgcggttg aatcgcgttc acttgctctg atagctatac
     8821 cacccagtgc ggcgcgatat tcttttacat tgccattcca ggaaggcgac aaataagccg
     8881 acgatgtagt aggagaaact ttaacataac ccggcagata tttttgataa gcggcattca
     8941 ggtcaatttg ccagcttttc cagaaagccc gcagatccag tcggtaaatt tgcgaaagac
     9001 tctcgccggt ctgggataat tttgctccga gattaccata ataactatca tagcccagat
     9061 tttcgcctac aaaacgccag accgtcgtag tatcagctaa tttaccacta atcaacatac
     9121 tgaaattcat aaagccataa tcgccccggt aataatcgaa ccgcgaaaat actttatccc
     9181 gcaaaaaata gcgcggtgtc atttttatgg cagtcgcttc caattcaatc tgatcgaaac
     9241 gcggattcag ccaggttaat ggcgcttgcc cgtaaaccgg atcagtcaac agcagaccat
     9301 tattccgcat agtgagagcc gtaaccggca aaccgaaagt ctggattacg cctttgactc
     9361 cccaatcgtc ataaggcaga aaaatgtaac tacttcgctc aaaaatacga tcgccaatat
     9421 tgtaaatccc aaccgattgg gtttcagttt caacccggct ggaatccgct tggtacggat
     9481 tgtcactcat accggcttct aaaaaaattg aaatccaacc gacaatcagc aaaagcctga
     9541 gagctttcat ttcagcgtct ttcttccctg aaataaggta ttgcccgagc gatccgctgg
     9601 gctgttaccg gaactaaaaa cagaaaaaca atcagattaa cgattatgtg aataatactg
     9661 aatgccaaac cagccgctaa ggtcgtcacc aacccacgcc aattaaatcc cgctgataag
     9721 ggataggcaa tagaaacgca aatatcataa aaaattgtca aaagcccacc gagtaatcca
     9781 attgcaataa aatttctcag tcgcggttgc cagcttagta caaaatttcg gcaaagtccg
     9841 ccgacaccac caataatgac catcgccagc acttgagcaa tcaacattgg cggaaaaatt
     9901 agtcccgagc ccaatggatt aagagccgag aacagaaatt cgcccaatcc gccgataatc
     9961 agcccggact tccagcccat caaaaccccg gataaaaata tcagagccgt aactaattcg
    10021 acattcggaa cgaatgccag cgcgtagccg accccgacga tcagtgctat aaaaattgcc
    10081 gagtaagtta tttttctaat ccttgaattc acaaaaccac cattaagtcg ctgcaattta
    10141 caaagcgacg ccgataattc aaggattctt tagctattct ggtcggattc gcgctcgcct
    10201 ttgaataata cccgtttgaa ataaccacct ttggcagtcc atttgccctc attcaggtca
    10261 gttgcgatgt catttttcat ttgattaaag cgcgtatcga gttcatcaat ataataaact
    10321 accaatgctt ctaaaaattg aggttcacga ggcgattgaa attcgtgctt gccctgatga
    10381 gaaagaagaa tatgctcgag ttgcaaacgt aaaatttcag gaaaaccatt tattgctagg
    10441 cattgatcac ggacgatatc tcgtcctaat acaatatgac cgagaaaata cccggcatcg
    10501 gtataagata ccgccattcc agcctccaac tcaactaact tgccgatgtc gtgcaacaaa
    10561 atcccgctca ttaccaaatc gcgatcgagt tcggtatagg cttgacaaac tccttctgct
    10621 acctgacaca ttgtatagat atgttccaat aatccgcccc ggaaagcatg atggagaatc
    10681 atcgaacccg ggtggatttt caactgttcg ccatattttt cataaatagc gccaacgacc
    10741 gccttgagga agggattttt taacgattgg attatttgtt gtaaatcctc ccacatactt
    10801 tcgatatttt tactcgtact aagtattaaa tcaccggcat cgtagccttc ggcgcggtcg
    10861 cgctccggga caacacgtcg aattttttcc actttgatct gaagttcatt gttaaaaatt
    10921 gtgaccgtgc cctgaacttt aacggcgtcg ccagcttcga attgctcggc taaattctcc
    10981 acatcgtccc agattttagc atttatcttg ccggtgcggt caactaaaat caggtcgaga
    11041 taaattttat tttccttcgt ccgccgaacc gccttcgaga atacgccaaa aaacgaagtg
    11101 acccggtcat tttctttgag atcgctaata aatgtcttgc tcattttttc cctctgaatt
    11161 ttccagaatt aaagctaatt agactcccaa agtcaagagt ttttagtaaa aatttatttg
    11221 ctataggaaa agaccagccg cttacgagca gaaattgatt tttaaggcga gttacttgcc
    11281 ggttttatat ttccgatttg gtaattcgct aaatgttcta aatttagcta tgtttatgta
    11341 tttcataacc gattcacgaa gtctcgctca gtttgagcgg tatttggaaa taagaagaaa
    11401 tttggtcctg aaataaatca attaacttag ctctgactaa tgatagggat aaaaaataat
    11461 gacattcaat ctgataatcc acaatcgggc agaggatttt tcccgccgat taaactctcg
    11521 tggaagctgg tgcttttcac cgtcattttg atcctgttcg tcaccgcggc tatcacttat
    11581 ccgatttatt ggcaaacgcg gcaggcgctc gaaaaccagt tgaccaatca tctggaacaa
    11641 aatatccagt tagtccggga gcaaattgat acttcccttg taaatctgct cgccgattac
    11701 ccggagttaa attccgtgcg ggattcactc gagactaaac ttaactggca agctggcaaa
    11761 ttttcagctc aagccattta cctgatagac gccagcggtc gcttactggt aatcgctgga
    11821 aatgaggaaa gcgcattaca atcagccacc ctgcacgcag tcgaaattgc cagcgctcga
    11881 aacgatcaag tgacctcttc gcccctgttt gccgatcgca ctggtcaata ttataaatct
    11941 gtttttacaa aaattacgga aaacaatggc cagccggttg tgcttggact ggacgccggt
    12001 gccgatttcc tggaaattac aggtaagcta cgcaaccaaa ttctaagctt aactgccatt
    12061 atattgataa ttagtattgg tctggtttta cttctggcgc gtacgatgac taaaccactc
    12121 gaaaatctaa ctaaatatgc ggaggcaatc ggtcagggtc gcagcgatct tccggttctg
    12181 aaccaccgac gggatgagat cgggttcttg agccgaacta tacaaacaat gtatggcaaa
    12241 ataagtcagc gtgagaagga aaacaaacag ttcttagctt cagtcgccca tgaaattcgc
    12301 aacccattgg gcggtatgaa aatcaacgct gaattactgc tggaagacac cgacaatctg
    12361 actaatgcaa ctaaatatgc cactgcgatc gctcgtgaag ttgaccgact ggcactgatc
    12421 gttgactctt ttctggcgta tgcccgtcca atcgaaacca atctgattat ctgtgatttg
    12481 aacgaattgc tacaacaagt tatcagcgaa ttgcaacgcg atttcccgtc agctaaaatc
    12541 accgtcagcg ggcaagcgcg ggtaaaagtc aatcccggca aaatccggct ttgtctatta
    12601 aatctgattc gcaacggtct ggaagccgct ccagaaatac ccatcgtgaa catcaatatt
    12661 acgaacaagc cggggcagac tgaagttcgc tttttcaata ccggtatgcc gattgcacct
    12721 gaaatccagt ctcagatttt tgaggctttc ttcagcacga aaccggatgg catcggtttg
    12781 ggattagcca tcaccaaatc tctcgtcgaa caacacggcg gccagatcta tctggatcat
    12841 tccaatacca gcggcacgga atttgtcttg attttaccgg agtaaaatat gaattcgccc
    12901 aaatccaatt ataccatctt gattgtcgaa gacaaccaat ctatgcgcga cggactgctg
    12961 gcttcacttc agaaggaagg ttattccgtg gcaggtgtgg ctgacggaca ggaagcctgg
    13021 agtctgcttc agaaccagcg attcgatcta atcattaccg atattaaaat gcccggcctc
    13081 gatggtctga ctcttatgaa aaaggtcaaa aacctggcgc cgatgactaa catcatcctg
    13141 atcacagctt ttccaacggt tgatattgca gtggatgcaa tgaaatgcgg tgcgcaggat
    13201 tttatcacga aaccatttgc gctggcggaa ttacgacaaa aagtagaagc actgtatcgg
    13261 cgtaagtttg tccaacttaa acccagccta agcgagccag aattcccgga tttgatcggc
    13321 aattcaccgg ctatccgaca aatccgccag ctaattgcga aaattgcacc gggcgatagt
    13381 ccgattctaa tcaccggcga gagtggcgtt gggaaagaat tggtcgcccg actcattcat
    13441 caagccagtc gccagaaaaa cggaccattt ctcgccgtca actgcggagc tttaactgaa
    13501 acacttttgg aaagcgaact ctttggtcac gaaaaaggcg cttttaccgg cgctcataaa
    13561 acccatatcg gaaaatttga acaaagtaac agcggcaccc tgttcctcga tgaaatcgga
    13621 gatatgtcca ccgccttgca ggtcaaactc ctgcgcgtat tgcaaaccaa acaatttcag
    13681 cgcgttggtg gtgagaaatt catcgccagc gacttccgac taatttgcgc cactaataaa
    13741 aacctcgaaa aaaagatcaa agacggcgag tttcgttcag acctttatta tcgcatcaat
    13801 gttattcccc tgacaattcc acctctgcgc gaacgcccct cagatattcc gttactcgtt
    13861 gaacatttac ttcggctcaa agccgaccga cttggtcgta aaataccgcg aatcgaaccc
    13921 agcgttatgg ctcgcttaat atcttattca tggccgggta atgtgcggga attggaaaac
    13981 tttatcgaac gggctttagt atttatcgag gaagacattt tctcggaaaa actatttaat
    14041 ttcggcgacc tgccggaaaa tactagttta ttgaaccaaa ctgacactcc agcggctcgc
    14101 gacctgactg ctcagatcag tcaaatcgaa cgcgaaatga tcattaaagc cctgcgcgac
    14161 cagcacggcg tcaagcaacg cgccgccgaa caattgcgaa tcaaacccag cactttgtat
    14221 tacaaattgg acaagtatgg cattaagccc gaggaatatg aataataatt ttacttcaag
    14281 aatcgcaatt tgcgggacgt tgattctaat cctactactg gcgaaaaata ttcaatcagc
    14341 gccaatagac agcctggctt actatcagac gcaatataac cagcaattaa ccaaactacg
    14401 cactctcgaa aaggattatg acaaactcct taacgacatc aatgctattg atgcccaaat
    14461 tatcaaaatc aataatgcca gcaacctcaa ttggagcgac aaaaggaaac tttcccgcct
    14521 aactgcccga aaggctgaaa tcaatgcaaa gatgatcccg atttataccg aattaaccaa
    14581 tcaaaggaca attctgaata ttacctttaa tcgctattat gattttctgt cgaaaagtat
    14641 agactcgact gtcaatcaat ataataccgt cggtgactcg cgccagcgcc aaagtttgag
    14701 cagaacctta tttgaattaa ttgaccagcg cggacaatta ctggcaagcc agcgccagtt
    14761 cagcgatcag aatgaattcc cgttaccgga aaaggaagac ctggtggcac taatttccaa
    14821 atacgatttt aatgaggcaa ttcgccggga tattagccat attcttgacg cgaaaatccg
    14881 gcagattgat ttaatgatcg ccgccgccaa cacggagaaa gaattacgcc aaagacttaa
    14941 tcaattgaat ctggaaatga atgccctgag cggcgaatct tatccatatc aaggcggcaa
    15001 ctttagcgac tacgagaact gggatcatgc gacaatgtcg ggaaaatctg cctatcgtaa
    15061 tcggaccgac aaagcggctc ttccggctac gactcaatct gtgaccgccg ccgactatct
    15121 accccttttt cagaacatac cgacgaacga tctgtctttg tatatttcac agctcgactc
    15181 actccgccga gattaccaga aacaattaca ggaaatcaac ggaaataagt aaattgccgt
    15241 tgaatgctgg cgctctattt tcgtaatctt acactaatcc tatttattgt cgttctgtta
    15301 ttatccggat tactccaggc tgatcagaag ttaagtggtt attgtaaaac cgaagccggg
    15361 atggaaacca atattctgga atcagactct acctcccaga atagttttac gacacccgcc
    15421 ctggctaatc tccattttta cctaagcaaa cgacgattga acttcaatgt caatgcgacc
    15481 ggcggattat tagcctatga ttatcgtcct gaaaacaaag ctactttaaa tatatcagcc
    15541 caggccaatt ataccgtcag ccgtaaattt tttctggctt cgaccgtcaa ttcttttcaa
    15601 aaatggtggt tggaacagga ttatgcttat cataataccg attggctcat cggtggaggt
    15661 ttgaatctca gtcattggaa tccgtcgcta tccattattc tgtcccaaaa tttattccgt
    15721 catagcagta gcctagataa tcaccgtatc ggcggactgg tagaaatttc tagctcactc
    15781 aacccaagcc tgacgttttc ggctcaaagc ggctattccc aaattgatta cctccatcgg
    15841 ccgctatttt cagtcgagaa ctccgttttg gatactacag cccggcagaa ggatctacta
    15901 ttttttggtc agatcggact tgagcatcgt cgccaggctc tcagcggtgt gcgcctgaaa
    15961 gttattacag tcgtctccaa taatgattac tcggaatatt acggtgtcgg cctgaattat
    16021 tacctatccg gaaaaattgg gcgtgtctat tgtcaggtca ttgcagatgt tttattgaaa
    16081 aaatatcggg ctgatctaaa tcaatatttc ctttattaca atcccgatcc ggagcagaac
    16141 atacagaatc aactgctcgt cgggtgggaa ttgccgctct ggaaaaattt agccctcact
    16201 ggtcgagccg caataatacg taatgaaact cattacagcg gcatatatta caataaatgg
    16261 tttgttagcg gcggcttgat ctaccgtaag gactaagcgt cttaaaaatc tcagattttc
    16321 tatgttagtc tcagaaagct gagggtttgc ctataccgga cagattttct ccgtcggaag
    16381 gtcttggaat gctatttgtc ataaataaat tgtcgttttt aagtattgcg aaaataaatg
    16441 atgaggcttc tatggcaaac tacaaaatta tgattctgac actgctttcc ctgcttactc
    16501 tgaaccgctg tcctgagaaa attaccggcg gtgacaaacc agaaccgatc cgtgaattga
    16561 gcgccattga aaagaaagtg acagaagccg acaacaactt cggtctgaag ttattccggc
    16621 aaattatttc ctcagagacc gataaaaata tcttcatttc acccctgagc atttcgatgg
    16681 cgctcggcat ggcttataac ggtgcggacg gtgaaacccg cgctgaaatg cattccatcc
    16741 tagagttcgg cgacctgaca gccgcagaga tcaatcagtc ctatcgtaat ctgatagaac
    16801 ttctctcgag tgctgaccct gatgtaacat tcgaaatcgc caactccatc tggtatcgag
    16861 caggtttttc gattctgagc gaatttttgc agttaaatac cacctatttc gatgcggtcg
    16921 ttcaggcgct ggactttgac cgcagtgatg cgactgatat gatcaacgcc tgggtgaatg
    16981 aaaaaacgca cgagaaaatc ccaactatcg tagattcgcc gattaatcca aggacagtga
    17041 tgtttctcat caatgcaatt tatttcaaag gtacttggac ttatgaattt gacccggaaa
    17101 gaacacagga cgcgccattc tacctaaaag acggcagtca gacgacctgt gagatgatgc
    17161 gccacaaagc cacccatggt tactatacca acgaccagat tcagatcgcc gacctgactt
    17221 acggtgaaat tggcttcagt atgactatta tcctgccaaa tgccggagta gatgtcgatt
    17281 cgattgcggc aatgctaacc acggaaaact ggacagaatg gactaatagt ctgaccgaag
    17341 ctgaaatcaa tatttatcta cccaagttca agactgaata cgagattggt ttgattccaa
    17401 ctttgcaagc tctgggaatg attcaggctt tcagttcttc tgaggctgat tttgacaaga
    17461 tcaacccaaa ttacgatctg tatattagcg atgttaaaca caaaaccttt gtcgatgtta
    17521 acgaagaggg caccgaagcc gcggcagtga ccgccataac ggtcggatgt acttcagccg
    17581 gtccaaaaga gatttatttt atcgttaatc gtccgttcat tttcgttttg cgcgagaaaa
    17641 gcaccagcac gatcctgttc atcgggaaaa tcatgaatcc ggtttcataa taaggagaat
    17701 taaagatgga aaaaaccact cgtaattttt taatacttat aatttttgtg gctgggttaa
    17761 taagctgttc ggacgtcacg gctccggatt ccgtagaaat gccagagtta ccgagaagca
    17821 tcagtaaatc cgaacagacg ttaattgaat cgagcaatgc ctttggcttt gaccttttta
    17881 aaattctatc aaataaagcc aatggcaata acctgttcgt gtcaccggtt tcggtttcga
    17941 tggctttagg aatgaccgcg aatggcgcgg caggttctac tctggaggct atgcattcga
    18001 cactcggctt cgaaaatttg accgaagatg aaatcaacca atcgtatcaa agtctgatca
    18061 atttactgag atcgctcgat tcgaaagtca aattcaggat tgcaaactcg atttggtatc
    18121 gcaacgaatt cactgcttta cccacattta ttgacgttaa ccagacgtat ttcgacgcgg
    18181 tcgttcgggg tctcgatttc aatcaggatg ccgccgttga taccatgaat gcctggatcg
    18241 aagctcaaac cgatggattc atcaagcagc tcattgagaa accaatcaac accaatacga
    18301 taatgtttct gctgaatgcc atctatttta agggaacctg gacatatcaa ttcaacgaag
    18361 aacacaccca ggaggctccg ttttatctaa ataatgacaa cacggcgaac tgccagatga
    18421 tgtcactcag tagtgatttc gattatgccg agaccgacga actgcagatt attgacctgc
    18481 cttataacaa tggatttttt actatgacgg tactcctgcc gcaatccagt gatgatttaa
    18541 actcgttgat tagtggcatg accgatgaaa aatggaataa tctgatcgca ggcttatcca
    18601 cgacaactgt cagtctgtat ttgccgaaat tcaaactcga atggttccag gtgctaaatg
    18661 atcagctgtc agaacttggt atgggaatcg catttgataa tcgggctgat ttcagccgta
    18721 tcaacgacca atatggctct gaattatata tcagcgaagt cagacataaa tcttttattg
    18781 aagtcgatga agaaggcacc gaggccgcgg cagcggcatc cgtcgagatt acatacaaat
    18841 cggtctcgga tgacacagaa gacatcacgg taatgcgcgt tgatcatcct tttatttttg
    18901 ctatccgcga gcgtgcttcc ggcacaatct tgttcatcgg aaaaatcata aatccggttt
    18961 cataataaag aggaccaaag atgaaatatt ttgcgatctt cttgattctt attcttttat
    19021 tttactgcag ctgtaaatac tctacttcgc ctaatgaaaa tcctgattgg gtagaaaatc
    19081 ttatcacaaa attccaaaat gaaccagtcg gtaatccccc acagtccatc tggcaatata
    19141 aatacaaagg tcaaattgta tattatattc cagcgcaatg ttgtgatcaa tttagcacct
    19201 tatataatgc agagggagat ataatttgtg cgccagacgg aggcttttca ggacgcggcg
    19261 atggagaatg ttctgatttc ttccaggaac gaaaaaatga aaaactcatc tggcaagata
    19321 gtcgcactcg ctgatatggt ttaaactctc tttaacagga ggtagcatga agacatacaa
    19381 attattcttt tctcttaaca tcttcctgat gcttcttaat attacttttg caacggttcc
    19441 cgatccggtc acccgccagc ctgcgatccc gtatttgatg gaaccgattg gttttcgcac
    19501 gatgcagatg gcgattttcg cgccggaagt cagtggcatg gttccggaca agttcagcga
    19561 tctgagctgg aatccagcct atattttgcg cgaaaaacaa aagacttttt acattgactt
    19621 caatctggaa aacagtgtta cgtcctcgct aaatccgctg tcgtccggga ttctgacaga
    19681 cagttattat cttgcgccat cgtggtacag tcagaccagc gttcaggaca tcaatatcgc
    19741 gccgctttac aatctagctt tgattctgcc attgaataaa cgcatgagcc tgggtattgt
    19801 caaccgttca gtcttcgact atggccccta tcgaaactcg atctggtgga cgagcggcgg
    19861 ctggttcgat aaggctattg aatcaaattc caacattcgg tatgccgatt ttgaaccgca
    19921 acgtctcgaa actgacgaaa accagcagac ggttattgcc aaccaatccg aaattactct
    19981 cggctaccgg ctttcgacta aaactgatct cggcttgcac cttggccatt atatttttca
    20041 ccgcgacggt tacttgctgg attcgcaatg ggcaactcat ccacattcct catacggaat
    20101 tctgaatgac gaagatctgg aaattaatgg ctgccattat gatttaggcg ccggtttttt
    20161 gtaccatccc gatgaaaaaa cgacctatgg gattagcgtc ggagcatttt tcggttccgg
    20221 ccaggaatct gaaattgccc aggatacttc ggactcgttc tcggaaacag ctactaatac
    20281 cgcatattat tgccttagtg aacacacttt gaataccgat ttgacctata aaacttccgg
    20341 agtaaaacca tacctaacct tgattttgga aaagaaatta aataaaaatc tcgtcctgcg
    20401 ctcatcattt tcattgacca gtaaaaatca ggacgttaac actcgatacg ccaccgacga
    20461 taccacattt agcgaccgaa cctacaatca ctggattacc gatactactt acgctttccg
    20521 acgttatacg gctaatagcg ccgcggaatt caatctgaac ggttccggcg aagagtcaat
    20581 caatcaatgg aaatttatga ctgcgctgat ttacagtctc gaaaatgatt gggcgttttt
    20641 cggtggagtg cagatttcac gaaaatggtc acaggtagat ttcgacgaaa gatcgaatta
    20701 ctactccaat tacttcaccg agtattcgct ttacaacgcc agcacttacc gtaatctaga
    20761 cacttacacc aaaaaatatt cctactcgca ggaaaccaaa gcctggaatc taagcgtgcc
    20821 aattggagtc aaagcctatc ttgctaaagg attctcagtc ttactcggaa cggatattaa
    20881 tcttactctg tataaggaaa acagttacag cgatctactt tatccgaata ttgtttcaaa
    20941 atattggata aatgacgcct tagccaatga cgatccggaa accaatcgct acgagagata
    21001 tgactccaat ccgcccaaaa ctcttacccg cactaatgct gtccgctttg gcatggttta
    21061 cgaacattca tccggcgcaa agcttttcat tcgctccgat ggcgatatta tgaataccaa
    21121 cggctggacg ctgggtttcg agatgaactg gtgatgttga ttattaataa atttgcctat
    21181 aaatttcaat cagataaaaa cgaggcgaaa aatgaagcgt tttagcgaat tattcaaact
    21241 tgccctatgt ttctcttttt gtttcctcgg attgcaggct caatcccaga tgattgccaa
    21301 catttccgag gatgttcaaa cggattttgg gatttaccag ccatatactg ccaattttac
    21361 ccctgacgta ccgcttttta gcgtcgaacc cgatttcagt aatgttgaaa atttttctga
    21421 cttctatgga ttcagcgcgg tggactcagc cctgttactt caaaaccact tcacagtccg
    21481 cagaagtcag ttcaaacaat tatatgatat ttacaacgac tgcacctggg atggcacgcc
    21541 gcttttcgtc actaccgacg cggttctgca tatctatcat gttctttatg ataaaatact
    21601 ggctgaaatc gaaattcaaa aattcgtacc ggctctggaa cttttgacca aaaccctgat
    21661 tgacagcacc caatcgcaat atgatacagc aaccgaaccg gaaattaaag agacgttacg
    21721 gcgcaatctg gcgtttctgt gcgtgtcaca gaaattattg aaaggcaatg atttcacagt
    21781 tccagaaccg gtttccgcgc ttgtggattc ggaattgacc ttgatcgcca atcatgacgg
    21841 attttacact ccgcccgtct tgggaccttt caatttattg gattatagtc aatttattcc
    21901 ccgcggacat tataccacga atgacacgct gacggcttac ttcaaagcta tgatgtggca
    21961 gggctggaca attttcacca tggagccggc aaaattcgac aacctggcga ggagacacac
    22021 cctccaggct ctgttgctta ctcaaatgct ctttaatctg gacgcgaatg gaaattcatt
    22081 attggatttg tggaaaatga tctacgagcc gaccgtattt ttcgtaggaa aaaccgacga
    22141 tcccaatatt ttacattata aaaccattgc cgcacaggtt tatggctctg attttctttc
    22201 tctttccgct gacagtctgg ctaattcgac tctgcttgaa aatttcatga ctgaagctca
    22261 aaaattgccg gaaccgaaaa taccaaactg gatttacggc tcttttacaa cctacaaagg
    22321 gttcaggctg atgggtcagc ggttcattcc ggattcatat atgtttgctc atcttgtact
    22381 accagaagtt ggaggtcgag ctttccccaa aggcttggac atcatggcga ttctcggttc
    22441 ggaccgggct tacgatctgc tcgattcgct ttataacgaa actgcctatc cgaattatcc
    22501 tgaaaaaatt accgaattca agaccgaatt tgcggctatt tcaccagccg aatgggcgca
    22561 gaatctgtat tggaattggc tctattgcct gatgccatta ctatatgaaa aaggcgaagg
    22621 ctacccgttt ttcatgcaaa ctatcgcctg gatggacaaa gaactcctga cggctctggc
    22681 ttcctgggca gaactaaggc atgatacaat cctttatgcc aaacaaagta tgtcgccatg
    22741 tggtatagct cccggtccgc cgaagagtta tgtagaaccc aatccgcatc tttacgcccg
    22801 tctggcaagt ctcacgcgct atacaataga cggactgcag gcccgggact tgttaactga
    22861 aaaatttcgg gataaacttg ctctttttga atccctcctg cttttcttac aggatgtttc
    22921 gatcaaagaa ctggagaata caccactaac agatgaggaa tacgccaata tttattgttt
    22981 tggcaaagtc atgcaaaaat tggtctccga aggtgaggat ccctggcact atgacagcga
    23041 tgacatggcg gttgtcgccg acgtgcacac tgattccgac tccgatcagt gcctggaaga
    23101 aggcgtcggc tatccgctgg aaatcaatgt catcgtgaac gagggcggga ccattcgcat
    23161 cacacgcggg gcgattttct cctactacga gtttattcag ccaatcgcta atcgactgac
    23221 tgacgaagcc tggcgcgaga tgttagcgag tgaaacgccg ccgactctgc cgcaatggta
    23281 tgatggtttg atggacatga atgccagtca gccgtctttt atggaaaatt caccggataa
    23341 tctctatcat ggcgaatttc aggccattga gaaacctgat gattctcaaa aacccaaaac
    23401 ttttcaagtc aggcagaatt ttcccaaccc cttcaatccg ctaacaacta ttgaatatga
    23461 attgccacag tattcccatg tcaggctcga aatttacaat ttgctcggac aaaaaatcac
    23521 gactcttgcg gacaatcacc agccagccgg tcgttatcac ctgatctgga acggccgaga
    23581 ctcgcacggc aacaacgtcg ccagcgggat ttatttctac aaattgactg cagggatttt
    23641 tacgcaagtt aaaaagatgg ttttaacgag gtaaaaacct tctaataaaa tatccccgta
    23701 atcctggcat tgtagaattg ttcactaatt ttcgatatta atcaaaactc ttttacctta
    23761 taccaaattt tccgtaaact ccattcgatt ttatgcttaa gctaatttta aagattgttt
    23821 tatttataat tttgattccg gtagtaattt ttgttggatt atatattgcg gcgccggacg
    23881 gttcggaatt tgtccataaa aatccggaaa ccaccgcttt tattcgccag cgtacggccg
    23941 agtatcagaa agccggtaaa aaagttgcac tccattggca gtgggttcct atcactaaaa
    24001 tctcaccgaa tattattcat gcagtaattt tagctgagga tgctcggttt tatgaacata
    24061 ttggtttcga tgtggaagcc atcaaatttg cactcgaaaa aaataagaaa cgtaagaaat
    24121 atgcctttgg tggttcaaca attacacaac aattagctaa aaatctatac ctttcgacta
    24181 aaaagtccat tccgcgaaaa gttcaagaat tgattattgc ctttaaaatg gaacgtcatc
    24241 tatccaagcg gcgtattctc gagttgtacc tgaacgttat cgaattcgga cgcggaattt
    24301 acggcgtgga agccgcctgc cagcattatt tcggcaagtc tgcggctgat gtcagtattg
    24361 acgaagccag ccgtttggtt gcaatcatgc ctagtccgcg tcgtcattcg ccctatgatg
    24421 gatccaactt tactgaacga cgacgtaagt ggctgttgaa ttggttatac aaaaccggtc
    24481 atatcgactc gctcgattat gttatcctct acgaaggcag ggcagacaac ctgctggagg
    24541 tggttgattc taccggaata gatcagttta tgcaggaagc tatagatgat tctttaaaat
    24601 ctgatttaga atatatatcg cgatatctcc ctcttgataa caaaatccaa gtcagtgatt
    24661 ctataaaata ataccaaaaa tcaattttta attagacatt tttaatttct aaacttgcta
    24721 cattttgaag tatgattttt aatcaatttt ttcgttttat taaatcacaa ataatttttc
    24781 tttgttttta ttcataccaa tattaaaatt aatgcacatt tgtctgggta gacaaggttt
    24841 tttaaaatta ttataaagga gagtaaaatg aaaaaattgc tggtaatttt gatggttgcc
    24901 agtttggcgc tcgtcacggt gagttgccag aaaaagacca aagtagaaga ggcaccggct
    24961 ccggtcgaca cgactactgc ggttactgat actgttaatg cgtaatcatc gttaacagaa
    25021 atcaccgatt aagtttaaac aaaatatccc gctccggcgg ggtattttgt ttttagaact
    25081 aattgtaaac tgtataaaaa tcaagccaat actagagttt ttattcattt tgccaacgtg
    25141 gtaaaagtac atcccttttg gactactttt cggtaaataa caaatcttct aaaccactcc
    25201 cggttcacaa aatccttttt attacacgta gcgaagcaat ctttattgct atagaaattt
    25261 aattttctgg aattagtttc tcatctcttt ttgagatatt gatgataagg ttgagaaccc
    25321 cggttaagag tacgagatat acgactcccc aaatgattgt agctggtttt ttgaaaatag
    25381 cagctacaat aggagaaaag ccgaggatga acacaggagt gaagcccatt ctacggatac
    25441 aataagcaat gagagccgcg ggaatacagc tgataagtac ttcgagcgga ataaagcata
    25501 aaagaatccc aagagcggca gaagcccctc tgccaccata aaatttgtag tagatcggaa
    25561 aacaatgccc aataacagca aaagaagccg caaatatggt tggaaaagta tcaagtccaa
    25621 tgagtttgcc gatgacaact actacgaccg cttttagcgc atccgctatt cctgcgagca
    25681 agcctattcc tttgcccaca ttctcaaata catttaacgc tccggaattt ttgaccccaa
    25741 ccgttctaat gtcttttccg gtgcgtacct tggtaaaaat atagctaaac gagatagatc
    25801 ccaaaaaata agcaactatg gcaagaatta ctgtttggta aatcattatt ctaatttctt
    25861 attttaagac aaaactccca ccccacgtac ctgggcctcc atgaacagcc tcaacaaatc
    25921 tggcatgtcc atgaacgagt cttcgataag tgaggttatc atttatttgc ttttctaagt
    25981 ccttgagctc atcggcatca ctgttttcaa aatgcagtat atttataagc tcaattttag
    26041 ccaccttctt tagaaccatt ttctgtttaa tatgattgat aattatctta ttagcggctt
    26101 ggtatgttct tccttttcct acaggtaaaa atcctgaatc agtttcatcg ccaaataacc
    26161 ccactactgg tattatcctc aatactgagc cgagcagaga ctgtgccctt ccaattcttc
    26221 cccctctata aagataattc aagtccggca cacaaaaata cgagtaggta ttttttatta
    26281 cttcgtctat gcgcgagaca aattcatcta cactgtccga ctcttttagg agttctataa
    26341 cccgcagaag taccgcccca actccggaag aggcttgttt cgtatcaata ttaataattg
    26401 ggataatgcc ttcatagtct ttgcggattc cttctgccat ctggactgtt gccgaagaca
    26461 tattacttcc cattaacaca tgaacaatca gatcatattt atccttgttc ttttcaatta
    26521 cctcaacgat gtcttttctc agcaatgcct gagagcgagc cacgattttc tctttcttga
    26581 aacgctcaat gagatattga gcagtgtgtg tttcgtcttc aaaatattct ttttcatcaa
    26641 ttaccacagg aaattttact accgcaacgt ccgggtaatc aatttgctca ttttttaatc
    26701 cgagattatc gcctgtcagt ataagtattt tctgatccat ttcgattctc cttattcaaa
    26761 ggtaatataa tagttgtagc gattttggcc accataatat atttcaaact cgcattgcgt
    26821 gaatttttgt tgcaactctg caatcagcga tccgttgttt ttttccattt tgcctctgta
    26881 taaggtaata agcgattcat tttccctcac tttttctatg gaaagtttga taagatcaat
    26941 caagtttgaa ttatgggcaa ggatgctttt attatgtagg cagaaatagt tgcctttagg
    27001 tattgtatca aaatcggttt taaactgccg cgttgctttt gctatttggc aaaacctaat
    27061 attatcgagg ttttcccgca tgatactggc atttgaaaaa atatccattt caggtgaaaa
    27121 tgtgtacatc atactgatta tctgaatcac actctgggtt tctataagtt ctatattact
    27181 tttgcatagc gtaatggcag atttgagact ggcgaggata tctgagtcat cagccgcaac
    27241 aatgatgttt tttgccctgg ttttgaataa ggcattccct atttggttaa cggaaggttt
    27301 tgcttgctta tacataagaa tatcggttgc tccgaggctt ttcaagattt tagcaaatcc
    27361 ctctccattg acaataagta gcaccgccag atcgcgttca tagaattctt tatcttccga
    27421 tataaggttt ttatgctgaa ccttcatatc gtctatttta gtcgctgaga tattcccaag
    27481 gttttctaaa gatttaagaa ggtctttagg tttgttagta tgaatatggc acttataagt
    27541 attgctagaa gaaataatga ggatgctatc tccgaatgat tccagtttct tttttagtga
    27601 atcttttcct atttcatctg attcgaggat gaattcagtg caataccgat attgtatatc
    27661 cgggttccag gtttcatcca gttcattact ttgcgctaaa atctcttcgg ccagttgatg
    27721 gttattattg cgctggagca aattattaaa cttggtcaga atatttgcga aggaaatatt
    27781 gttaatatta tttagggaaa tattcaatgg tcgtaatttt ttccgttctt ctccagactc
    27841 atattttcgc tcttttataa ttgaacgaat ataacggctt gtcgagagaa tggtggcaat
    27901 attcataatc ggacgaatcc ttgtacccgt atattccagt cccgtagcca ctcctttgat
    27961 aagcaccaga aatcccatag caccggaatc caccacacct gcttttttaa gaacggggag
    28021 catttcagga gtcttctcca acgtttcttc cagatcgggg acaatatctt tgataataga
    28081 aagggcatcc ttgccatcca ttaccagttc agaaaaccgc tctgcaaata ctctcatcat
    28141 agtgagcatc gtgccttctt tcgggttggc agtctcggca taggcgtatt tatatcccga
    28201 aataagtgct tgcgcaatct ccgtgttagg tattttctga tctggaggcc agtctgctag
    28261 ttttttcgcc accccttgga taaatagagc cagaataaca cccgaacacc cgcgactatt
    28321 tagtgtcatt ccttcggcaa gggcggttag atattccgat aaagttgatt ggtcggaggc
    28381 ataatttttg gcatgagcca agccaccctg caccgtaaga accatattaa gacccgtatc
    28441 accgtcggga acgggaaaaa cattcaggtc gttaatccga ttcttttcaa tagagagcac
    28501 tgtcagtgcg tcttttagaa tgctattgag aactgaagcc gttatttcca tttccactct
    28561 ctcaaaattt caccacactt caacatttag ggattcaaat aagcaatata gtttatttta
    28621 cataatagct ttgattgatg caaattcatt tttctatatt ggtgtaaatt ttaaagatgt
    28681 ggtatccact ttttagaaat ccagattatt ggaaaatagc tttcggtaag gcgaaagatg
    28741 tttgagtgta accaaacacg ccaaagttca ttaaaaattt ttcgccataa tgacgcaaat
    28801 caaaaggaaa agcggattcg gcctattcgt tgtcgtcaat cataattgcg atcaatcaaa
    28861 ataaaagcta aacgttgccc ttgttccagt gcgattacta ttcaccgatt tatacctgtt
    28921 agcttttgat aaatcgaggt aagtaacgga cggaccggct gagtaaaata cttccgtgac
    28981 agccctaact aacattcatt aagatgagta aatttcttaa aaagattgag gttcgctgaa
    29041 atcttctcaa ctaactaaaa ctttttccca ctatattctg ggaaaaaata ctcctcctat
    29101 gcctataagt gatatttttt aatttataaa tattggctga aaagtatgct tttaactaaa
    29161 aagcgtactt aaacttcaga tagacattat cgtttttagc aaacatccca aaaatcgtgt
    29221 cagattttcc cgctagtaaa tctactcccg ccgtgatcgt aaagctatcc gcataattcc
    29281 aatcaatact caagcgcgtc aatgaagagt aatcctcgaa attatacatc actaaaatta
    29341 gcggtgaaac agtttcatta acaaaatgac ctctcagcat caccgtacca atagtgacat
    29401 tttcatcggc ttgaataccc tctttataat ccagaatccg ttcgttaatg ccttgaacag
    29461 ataaatccaa agccgatgtc aactgataat cgccaccaag cataaattgt aaaaaaggcc
    29521 tctcgattat cattcctttt gataaatagt tcatatcttg agtgttgaaa tggcgctcgg
    29581 gataatagcc gccttctcca cggaacacaa atgccccgat tggcttggag aaattcaatc
    29641 cgaagaaatt ctgccaggga tgcgtcggag tcaaatctac tcgaattggt aaacccgaag
    29701 agtcattaac aagttcggta aaatacacag gcttatcttc acgactacgc agatataaaa
    29761 atgccaggtc ggttctcaaa aaccaggtat ttaatttcaa accatattca gtatttttta
    29821 acgataggtc aggaatttgt tcattctgat aatgcaaagg aagtacaatt ttgcccagcg
    29881 gactttcgta ctccatcatt tgcggccttt tataacccca agcaccctca aacagtagat
    29941 tcatcggctt gaattccggg attacgagca cttccaacga gtaattgctg tagtgtaatt
    30001 tggcattcag catagtcgtt gccatgcgaa tatcgtcaaa atcctgtagg agaaaatagg
    30061 ataaatccag cggattgacg atgtcgttga taaaatagcc gtcggtttta ccccagacca
    30121 cctgctgttt gccgattcta aggtcgagcc aattgctata aatgtcaatg tacacttccc
    30181 gtaaattcgg caacgtcgtc gtgctgtcgg ctatgtcgtt ggtaatatca agcgacgcaa
    30241 aaccataaac gttttcaccg taagtttcgg tgttcaatct cagacgattg cgaatcaaca
    30301 taacatccat tgattcatta gtttgaaaca tatcctgatt gagaataaaa cctcgcagag
    30361 taatttgcgc tacggctgta ttgccaaacc agattagcaa aattaccagc cagaagattg
    30421 cccgtttcat cggatgcctc cgcgttcgag ggctgtaatc gtaaatattt ggtcatcaat
    30481 aggttcgttg attttagcct cgataatttt gagaatggtt ttgtgatttt cagccaaatt
    30541 ggtcatttca aggttaaact gcaaccagac gccggagaca tttcgaatat catcggcttt
    30601 gagagttttc atcagttgac ctttccggtc atagaaatct actttcaggt gcacccattt
    30661 atccggaatc acccagccga tttttttact gtaaatgtca tttttgtcta tcggaactga
    30721 ctcgattttg taacacttca cgccatcaat cgtctcttcg ccgagtagag tgtgtttatc
    30781 ctgatcaacg cttcgcccac ccatatcatc ataagtaaaa tccgtaccca tgaaatattc
    30841 attcttgctt tcaccagcga tgcgccggac tttttttaat gcgggcaggt aaagccagcg
    30901 gtcatcatct ttattgacat ccgagtagga aatcatcagt aatcccgtgc cacgaatgtc
    30961 ggctgggtat tcgaaaaata ttagaatttt atcatcaacg cctttgggat cgttaaccga
    31021 tttaacatat tggacgacat cccgctcacg ggtcttattt tgcttattga tcaagatcat
    31081 tttcgactta acaatccgcg tcttagtatc ctgctgtttc tcgactttca gcataatgtc
    31141 gcgcccggtc atttgggcta tagcggaact ataaaaaatc acaagggcga tgcaaattat
    31201 acttagagaa aaaagctttt tcatctaaac ctcctttgaa tcgtcaggaa attttccttt
    31261 ttttaatttc atataaatta acataattat cggagtaaat attattccta ggaaaaaagc
    31321 ctccgtaaaa tttttccgat aacgaaattc aaccacacca gcaatttgca aacagataaa
    31381 tacataaact actagaataa tgctcagatt ttccaggcaa aacccgccga aattcgacca
    31441 tatcccagac gtttcaatcc taacagtact gaaccaccga attaaccaga gaatcagctg
    31501 atatagccaa tagactacaa tgccaccaga aattatcgtt agcccgcgcg ctatattttt
    31561 atctttttgt tctattgtca ctgtaaactc cttttaccga atataaaagc cggtttggtc
    31621 aggactaata aagccggtaa aatcgtcaag gcgccaaaaa gacaaacggc aattgaaaac
    31681 acaatcagaa atccgaaaaa gctgatcggc tcaaatccgg ataacatcag gaccataaaa
    31741 ccaataataa ctgagatggc gttataaaca atcccctttc cactggttgt aagtgtgcga
    31801 atagtagcct cttcagcgtc agtgccgtta cgcgcctcca gtcgaaaacg atataaaaag
    31861 tgaattgtgt aatcaatccc gattccaatc ataatactgg aaattaaagc cgtcgcaata
    31921 ttaagagtaa taccgatatg tcccataatc ccgaacaaac acaaagccgc cacaattagc
    31981 ggcacgatag cgatcaatcc cgcttttacg gagcgaaagg ctatcgcaat aattagaaaa
    32041 acagcgataa ttgaaagtgc caaacttcgg atttggccat caacaaccaa ctccgataag
    32101 actccgataa gcggcgccat tccagaaact ttagggaaat tttctgcccc taaattttca
    32161 gcgatataat tctgcgtatc tttatacaat ttatatgagg ctttcgaact ggagtcgttc
    32221 actcgtgcca ggatttgcgc ctgggtataa tcgtaatcca caaagcgatc cagatccttg
    32281 gtatcgccag tcaatgaata gagaaaaaga tactgcgcta cttcctctct ggtctccgga
    32341 ataacataat actcggcact gtcgctgtga aaagcctgat tcatacgttt aagcatatcc
    32401 actatcgaat tggcgcaggt gacggtcggc tgactctcca gaaaggtgca aagattttct
    32461 atcttcttta aaaattgagg gtcttttata tcgccagtag caagtactga tatagttgaa
    32521 ctgccaccga agtttcggtc aattacttca ttgctttggc gaatttcaga agattttttc
    32581 caataggcaa ttggattagt atcaacgatt atacgtccga ttccggttgc gacgataata
    32641 attgccacca agcaaagtcc cagaaatggc ttatgataac gagtgaaaaa ttgcccccag
    32701 ctcggcagaa accgactggt tcgcttacga gaactggcgg ttttcaaggt taccggaaaa
    32761 ccaagcaatt ttaacgccgc cggaatgaaa gtcaggctat aaataaaggc aatagtcgtt
    32821 ccgcaggcac ataatatccc gagatttttt gccggtggta aaacgtgtga tagcaggcaa
    32881 ataaatccaa ccagcgtcgt aaatccggaa agcagaactg gcgtcgttag atacgacagg
    32941 attaatcgaa ttaaactgtc cttatcagca tccggatttt tgaggtgctc gacaaaatat
    33001 tgggtaataa cctgaatact ataactatta gcaatggcga tgagcattac cggaatcatc
    33061 atctcgacga ttgtcatttt aatattgaac aaaaccatta aaccgacagt aaatacaata
    33121 ctcataatta cgacggtaaa cggtagaaat gctccagtcc aactgtgaaa agtcaacacc
    33181 agcaggaaaa tcatcaaaat aataccatac ggaaagagtt tcttgacgtc cgcggccatc
    33241 gtcgccgata cctctcgacg agtcaaaggc aatccagcaa tgtgaatctg ctccggtccg
    33301 gcatatttat ttttaatagc gttgaaaatc tgataaacct gctcgtcact attttcatca
    33361 gtggcaactt tcaagtaggc aatgattccc gccttctgaa aatcagacga agccaaagtc
    33421 ccataaatca ttcgatttcc aataatgcgc ttcttcagcg aatctatctc cgactcggtg
    33481 gttggaaatt cctcgattag ttctcgcacc tcaaatccgt cggcagtgcc aactatatcc
    33541 ggaacattag taatcgaaat gacttgatca acgttttcca gcatttcaat ttccgaagtt
    33601 atcgcctcaa ttttttccag cgttgctgga gaaaagatat tatccgcagt aattgaaacc
    33661 acaactaaat cattgccgcc gaaagtgttt tccatatcct tcatattttc gataatctca
    33721 aaatcctgcg gcataagggc tttaatatcc ggttcgagct taatcttggg aatattccag
    33781 cccatcaata gagttaatag aatacagaaa gcaatgatcg ttcgcggata tttcaggtca
    33841 aattcaatta ttttgcgaat cattcatcat ccgaaaaatt aaaggtgaat tgtttctcca
    33901 tcagtccgtt cataattata tctacaaata tttttatcat ctcggtaatc gaatagggcg
    33961 cactaatgaa tatctccggc tgaaaggtgt tctgaacaat tcttacgatt aaatcggctg
    34021 tgaatttgag gtcaaatttc ttgcgcaata gtccgagatt atctgccttt tgaaaagaat
    34081 ccagaattcg attgattacc tcatgttcga attcttctac ttcctgccac actttgggat
    34141 aaaacaattt tacatcgaga attgtttgcg gattaatttg agagatatgc ttgaacatta
    34201 ttttcattat tccataaaaa cacttaagcg gctcattatc atttagctgg atagcacggg
    34261 tctcggtaaa caattcatga taaatgtcga gaagaacata ctcgatgaat tgttctttgg
    34321 tagggaatac tttgtaaatg gtctttttag cgatgtggag gtcagcagcc atttggtcaa
    34381 tattcaagcc gcgggtgcca ttggctagga tgaatcggcg agcgtaggta attgccggag
    34441 agagtttagt tttggcttct tcacccgatg gaaacgaatt ttgtttcata cgtttccaat
    34501 ctaaaatatt ttaaaattaa agtcaatagc taattttgca atgaatcaat ttaagattaa
    34561 aaactttctc cgtcgtaaac catctaattt tttacctaat aatttttatt tgaaatatta
    34621 aaattttaat gtataatagc gcgttttttt ac
//
LOCUS       DAOSMP010000024        34586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_1279_length_34586_cov_19.323970, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000024 DAOSMP010000000
VERSION     DAOSMP010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 34586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34586
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1279_length_34586_cov_19.323970"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..3345
                     /locus_tag="PKX09_05560"
     CDS             139..3345
                     /locus_tag="PKX09_05560"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HOU17324.1"
                     /translation="MKFKITLVFIICLVLSGMMNFGLAQSDQPLIFSFKQNHTVLYKI
                     AKCSNCDNDENFDVIGIAGLVDSHGQLVPRSTYLVHLEESENSDFTVQWSYNLPQDIK
                     GDFTDIAVINWNNVPAIIAVLNITEAGTGPDPGWLLLFEYSDGFSKEPTVRMATDTVF
                     PIRPRPSYLSVGDLDHDEQSDLIISSSSPNRSINIVTQAADTSALSLKLINYSNDLPV
                     LLGLQPFRALAANVDTEPGDEIVIFGGQQKLTVEVYRSDLKNPLSSYTFSNVNRNDIN
                     LDQLAAGDLDGDGLAEFILPLRSGGAQLLVKGVQSYKSGLLIPKKNKIAALNLVDING
                     NGLDEILLADAAGADIYRYEYNPGANLFDLDTYISFHYSNPVLTNVQLLSIAPVVSYG
                     GKNTGAIITPFLHRSFNQHGLCYWLLEETSPLIDESLIETILGSVDSVLAHKEFDQKK
                     GASSLTEMDLLKQQLGSLVGTEIELSPLPEGKGGPSGISADRQIYQPDILLHPGELMR
                     YNLNLPDLSLDDTKKLSVNIDAPSGMKFDLANKLFTWVPADSQLGLHKVTALISWDEK
                     KDLQTFTVYVNSIPKILTKIPIRDIIQIGETFSLQIEVEDANHDAIIFYKLLEGPAGA
                     SINQNGGLLWKPSFDQADWYDFIVEISDGYDSEQKSFALFVNHPVSIQSTAPKTTTIG
                     KKYTYQPVISDNNKGFYLSGYLLPPRIENWSQTGVLETQILDKNIRNNISKYIENYRK
                     SFPLFITPAKMNDVPQSLFQDIFIDSEKVVFIYNLRTAKGMEAGQIIETFFSNLKMSI
                     PKYSTPVRRYLYIFNLKEPIAGLTMTGEGLITWVPAANQFDYQLISYTVTDGFFSAEE
                     QAQVYVNYPPKITSKPVTIASVNTLWQYEVKVTDLNTDSKLTYELLSAPEGMVISPQG
                     IISWQPTDLQINAHNFTIKVSDGFAQDVQKVHLFVNVKPKIISVPKPVALTNLKYEYK
                     LEAEDANNDKLTYVAVRVPKYASFDPTTGMLVWTPRKDQIGANDIVLKVTDSHGGSTL
                     HEFQVHVFVNPARRHSFLRRTISLLTIAGVIYLVVIYL"
     gene            complement(3340..4395)
                     /gene="lpxK"
                     /locus_tag="PKX09_05565"
     CDS             complement(3340..4395)
                     /gene="lpxK"
                     /locus_tag="PKX09_05565"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOU17325.1"
                     /translation="MQLGALRWLLFPAAVIYGLITKLRNLFYDRHIFKAIKMPVPVVA
                     VGNITTGGTGKTPFVIALAKFLSEKGYRIAIISRGYRRKSRGTVIVSDGRQILTTPQQ
                     AGDEPFLMAGKLPGVVIVADADRIAAAHTAIENFACNLIIADDAFQHRRLARDINIVL
                     WDPKTPSWQKYLFPVGRLRESWHGLKRADYLVFSKTDKIIPEQRTFFEKFNPSITFFT
                     ASLKIVGLRTLSAENVSINTIAGKNVLAFCGLGNHIQFETTVRQLNCGKVTFLRFPDH
                     HHYTTTDLQKIIIKAEQMNCDYLVTTEKDAVNLPVNAGSLAIPLLVIEIELKLSDEIK
                     TAILQRLPPSSISGADL"
     gene            complement(4411..5535)
                     /gene="lpxB"
                     /locus_tag="PKX09_05570"
     CDS             complement(4411..5535)
                     /gene="lpxB"
                     /locus_tag="PKX09_05570"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOU17326.1"
                     /translation="MSAGKLFISAGELSGDMHAGKLISALKELNPDIEISAIGGDNMA
                     AAGAELLFHIRETSFMGFVEVIKHLPFIISMYRKTLQFLDEFKPDVVVLIDYPGFNLR
                     LAKALDRRDIPVIYYIVPQVWAWHQSRVKKIRRHVQEVICILPFEATWFSERGVRATF
                     VGHPLLDQNEISIPESNQKIFKSGKPLVGLFPGSRQQEVDKHLPLMIPSVKDLRQEFP
                     NLQAAVAVASDVNLEKYQKCYQFDWLSWHFNSTRSVIQSADVLIMASGTVTLEATLSN
                     KPFVVIYRVAPLTYWLGKKLIKVPFISITNLIAGKKGVTELIQHDANVENIKSEIMRI
                     LCDQSYANQIRDFLQYVCRLLGEPGASRRAAHIILNYLES"
     gene            complement(5522..6106)
                     /locus_tag="PKX09_05575"
     CDS             complement(5522..6106)
                     /locus_tag="PKX09_05575"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOU17327.1"
                     /translation="MDIRRLFFKIRGYTPIPLMLVLIYQSDMNFKLALIGAILALVGE
                     YIRLNGVRAAGGRTRTRNVGAKELCTWGIFAHTRNPLYIGNALIYIGMVFFAGGRWLL
                     PLMLLVFVYILFQYGLIISLEEETLTNIFGAEYAKYKANVPRLWPRLKPWNSGVQPKF
                     LSNRKLLRTERSTLLVFVIFALAVILKEWLNVRW"
     gene            complement(6106..7098)
                     /locus_tag="PKX09_05580"
     CDS             complement(6106..7098)
                     /locus_tag="PKX09_05580"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU17328.1"
                     /translation="MLKIGVVGAGNFGRWHIKNLQNLPNAELVGFFDNNPERASYISN
                     EFQVRAYPALEQLLDDCEAVSIVVPTHSHYSVAKAAIDCGRHLLCEKPFMETVAEAEE
                     IIQLARAKNLVLQVGHVERFNPALGALKQYQIDPLFIESHRIAPFRGRGTDVAVVLEL
                     MIHDIDLVLDLVTAPIVQVEASGAPILTDNIDIANARLCFANGCVANVTASRVSNKQM
                     RKLRIFQRDAYFSIDMLAGSTEIYSLSRDAGPVPDDTTQIMEVPDVNGAPRRIFYRVQ
                     QTEQGNALYNELESFVNAILTGQPPRVSGEDGIRALKVAIQIINSIQDNFRKIN"
     gene            7172..8743
                     /locus_tag="PKX09_05585"
     CDS             7172..8743
                     /locus_tag="PKX09_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOU17329.1"
                     /translation="MKFLIMEKVCFIQKNWLSQKFANGISLLILIMVINLWAELIAEV
                     PQVVIVHWNDFHSTNLPYKSSQADYRVGGYANLAGYFDSLRHLHPQAIILNAGDDFQG
                     SPVSNLTRGLSQILILNKVQPTAFTLGNHEFDYGIDNLRNVMTYANFPILNSNLFDST
                     KNDLLVQPYTIIQSGNLKVAIIGALTGDLFTLALPQNLKGIGILDPLTQIRQYVNEVE
                     PQTDLIVLLSHCGFEEDSLLAMQLAGVDVIIGGHSHTYLHQPKKVNNILICQAGSNGH
                     CLGYLKANIDRNQKIITSYNYELIETRLGRVKPSPIVAGVVDSLEASISAEMDKIIGQ
                     LITDWHRNSHGESNLGNWICDVTREYFKTDIAFMNSGGIRKDLKAGPIRVRDLWEISP
                     FDNTIVIVALTGEQLKFIIQYHVENPRDLLQVSGLSYEFNSKQKKLVKLTVAGKPVDD
                     QKIYTFATNNFVISQFERFFGLAPSAVKITPTDIIGRDILIEAIKAQPVINNHTEGRI
                     IDLANKKEEVNYDIQ"
     gene            8730..9161
                     /locus_tag="PKX09_05590"
     CDS             8730..9161
                     /locus_tag="PKX09_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17330.1"
                     /translation="MTSSSRILYSYIILGIALVAFGIATILGHFYIGLICLMLGMIIW
                     LIVTFAGNTEGQSGQIGVVMITAGFIGGLIIFIGIGLRPSIFGGHKLYIEGIVTALVV
                     LLIFMAIGLGFISLANLEKKMAFFNKELKKFYKLYMKEDNE"
     gene            9199..9837
                     /locus_tag="PKX09_05595"
     CDS             9199..9837
                     /locus_tag="PKX09_05595"
                     /inference="COORDINATES: protein motif:HMM:NF027727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5020 family protein"
                     /protein_id="HOU17331.1"
                     /translation="MALTQNLQLHYDFGRQHLTSTYELYKTDRLGATFLFVDFDYNNE
                     GSSGSRNASLAYGEIARYFQLPKMNDLSATLQYNDGLTNSGSFNSVWLGGLQYVFQIG
                     TQNFPIDFLLRKELNTDGLTFQLTYVWSYIWRKLELSGFIDIWNTGADAYPPRKIVIL
                     SEPQFWYRLTPALSVGGEIEISFNFSGAWHTNQVFSEDVIFILPTLGLKWLF"
     gene            complement(9966..10100)
                     /locus_tag="PKX09_05600"
     CDS             complement(9966..10100)
                     /locus_tag="PKX09_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17332.1"
                     /translation="MKYLKLIFKILAVITVIGLLILAVLLIIQKKSKSESVAEEPATE
                     "
     gene            complement(10116..10901)
                     /locus_tag="PKX09_05605"
     CDS             complement(10116..10901)
                     /locus_tag="PKX09_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17333.1"
                     /translation="MRKLFFSNATRVFFIVLGLCVLPIYLTPQSTKVKSILEQADKLH
                     DARDYQGTMTKLREAEKIEPNNPEVLWRLARAYFDIADQQPGVSEVQKANLYPGFEYA
                     KKCVELAPEVAGGHQYYAILIGQIGELEGTKQKITNSYAVREHTEKAIALDPKEDSNY
                     HVMGRWHFELADLSWAERQVASLIYATPPKASFEEAEQYFQKAHELKPKEIRHLLWLG
                     KTQLKLKKKDQAKKNLQLALEIPAQSDSDKVMQKEAADLLKKL"
     gene            complement(10983..11753)
                     /locus_tag="PKX09_05610"
     CDS             complement(10983..11753)
                     /locus_tag="PKX09_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17334.1"
                     /translation="MNRKQFFPFVFFMVLAGSLSAQGDLGIHQPVNLVTIPTAGILPR
                     GSYAVDCHLFPGGGLAGKISVGVFERFMFGVSYGGGGIIGNQKINWYNQPGVEIKYRF
                     IEESVKFPALLVGFSSQGHGNYIDSLKRYETKASGFYVVVSKNFNFLGNLGLHGGINY
                     NPIEKTDGDADPNFFLGIDKDLNSEIALIIEYDAALNDNHTELPLLGKGWGYLNAGVR
                     WRLVDKFTVELDFNNILLNRKDVDFMNRELKFTFTEIF"
     gene            12145..12321
                     /locus_tag="PKX09_05615"
     CDS             12145..12321
                     /locus_tag="PKX09_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17335.1"
                     /translation="MKGFGAVLVAISLILLVLEHLNLVDVVGIIGLLVGLYAIIFPDK
                     AEELYTKLKSGKLK"
     gene            12448..12663
                     /locus_tag="PKX09_05620"
     CDS             12448..12663
                     /locus_tag="PKX09_05620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOU17336.1"
                     /translation="MPIYEFECQNCGRVFEELVFLKNFSESEITCPHCGFKQAKKLIS
                     APALSRSSTCSSCGAKSCSSSCGSRSH"
     gene            complement(12828..13652)
                     /gene="proC"
                     /locus_tag="PKX09_05625"
     CDS             complement(12828..13652)
                     /gene="proC"
                     /locus_tag="PKX09_05625"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOU17337.1"
                     /translation="MKINQIAIIGAGNIGTAIANGLADSNRFKPAEIILTRRRIHLLD
                     DMQKRGFIIEKDNCQAVRQAKIIIIAVEPQQVNSVLIEIAPHLKPNHHIVISVVTGVN
                     TSQIVKKVGSGIAVVRAMPNTAIAVRESMTCLASDTASEAEIAITKSIFDTVGKTIVI
                     EEEKMTAATALGACGIAFFLRAIRAASQGGIEIGFHSQEAIDIAAQTARGAATLILTH
                     ANHPEYEIDRVTTPRGCTIAGLNQMEHEGFSSAMIKGITVSAEHAKSLYKNTNNSD"
     gene            complement(13966..15360)
                     /locus_tag="PKX09_05630"
     CDS             complement(13966..15360)
                     /locus_tag="PKX09_05630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU17338.1"
                     /translation="MTGNHARKIEYLKKKASRISIREGAAYGLFDGFGLRYITPYALL
                     LGMSNTLIGILISLPQMLGTLIQLLTLKFLEKGIHRRTIAWVGTSLQTLMWFPIILLG
                     WLHFNYNLNNMVISTGLMVFYSLMVIFGGIVGPAWSSWMGDIIPRQIGTYFGLRNRVV
                     GFTTILAMLSAGLILQHFNRLGQPFTGFAIIFLIAFTGRALSSALLKRQYEPEFHPQK
                     EYYFTIWQFIRKIPQSNFGKFVTYVALMQFAVYISSPFFAVFILEDLHFNYFQFTLIT
                     MTMPFVNMLLMPAWGRFADRFGNLRVVRLTGFCLPIIPALWVLAVFFIDRPVLAVLYL
                     ILVETLSGFAWAGFNLTTVNFIYDAVTRQRMAICVAYYNIFNGIGMFLGGLIGGLIAD
                     LPFQFHRFSALALVLMLSALIRLLVSAVMLPLIREVREVDHFDIKEARKRLAMLSPVD
                     ILRVLSLKLGNPRH"
     gene            15512..15587
                     /locus_tag="PKX09_05635"
     tRNA            15512..15587
                     /locus_tag="PKX09_05635"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15545..15547,aa:Lys,seq:ttt)
     gene            complement(16062..16706)
                     /locus_tag="PKX09_05640"
     CDS             complement(16062..16706)
                     /locus_tag="PKX09_05640"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOU17339.1"
                     /translation="MAIRAVIFDFDGVVINSEPLYLVAEQRLFDQYGIKVPDADWKYF
                     KGTTEGDFYQLIMAKYQIKTDAHKLIEDGRRYIKEEFNKGVDYLPGFLDFWQCIDGRY
                     RTGLVTSTSASFLEWVFRNTPVRNIFDLIITADDVRQAKPHPEPYLKAFESLGVQPEE
                     CVIIEDSMTGIQAAIASGAVTIGFRCDRNDRDMPKLHFYADNYTEITNILSKIG"
     gene            complement(16857..18476)
                     /locus_tag="PKX09_05645"
     CDS             complement(16857..18476)
                     /locus_tag="PKX09_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU17340.1"
                     /translation="MISKKDLIIKIALRFIDVPRVEIDNRINESLRDLGEFYQTEYCL
                     VSALLPDTKEALKIYGLEDKSEDSVKLLQNIALANSPWLKNQLSLKGTVIINDVNNLP
                     AKANREKQELQQLEIKSLMIAPLVYNDTSFGLLIVAVSKTSRRWLDSEIELLKIVASI
                     ITSALHRKKTEEKMAETMSLYHLFIDKINEGIAICHQERFIFVNNRLAEMLGYTVDEL
                     TGMEFHNILTNRGVEILKERERRQAIGKTVPKRYETSFKCRDGHTIEVEVSPVEIEYR
                     GKLTTFAVISDISEYKRLSLAKQALEVKWLKQQRLMSLTLMIGGIIHNIKNTLTVVMG
                     RAQMLKTRLPELKEPDIIVDNVRKIESLLTSFIDKMNLELENKKITINLSELLRNELL
                     FLETESFFKTQIEKEIVLRRDLPLITGHYSDFSQSLMNIIHCCVESMRDSPRKILKIE
                     TGISQDNIYVEIATTGQPIIPDNAEELFTPSFYREIPENEDSSDQKALIKYNLYNAYM
                     LLSSYKAKFSAFNNPESGSTIRVELPIESNN"
     gene            complement(18579..19199)
                     /locus_tag="PKX09_05650"
     CDS             complement(18579..19199)
                     /locus_tag="PKX09_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOU17341.1"
                     /translation="MSRTNNLIKAVFFDLGNVLVKVNLESAIPQIAELFRIPEKAVMQ
                     APILALEKKFERGQLTIAEYLVEVKNIYHIDHSIGVDDLERIWQTPFELNTDVWQIAL
                     KLRRQVPLFLLSNTNALHIRAIRRKFNILENMDGLILSFEVGATKPESAIYQYALRKA
                     DVTAEQALFIDDLKENVAGAERLGLKAHQFISASDLENILSQYSFK"
     gene            complement(19234..20217)
                     /locus_tag="PKX09_05655"
     CDS             complement(19234..20217)
                     /locus_tag="PKX09_05655"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOU17342.1"
                     /translation="MLLVSKLVRGNAVDSFHIGYAVVVDENGEILFAAGDPEYPIFIG
                     AAANPFQAVAMLESGALEQFNFNDEELALMCGTHTGEHYHLDVLNSILKKMDLNIDNL
                     LCGVHPPLDRQTYEQLAIQGRRPTALHNANSGAHAGMLAIAKALKYDLSNYTEINHPA
                     QQKIFEKIKLYSGKERIPVATDNSNAPTYLMPIYNLAIMYKKLVEGSDDYLRRVFHSI
                     SLHPKLIGGRAQFDTDFGALMGGRGISKFGMEGVRGIGLRNNENNPIGIAIKVLSGNR
                     EACDSMAVAVLKHKHLIDDEIAKKLDFYLSSAVKSYTDVEIGKFFTEVVAE"
     gene            complement(20233..20931)
                     /locus_tag="PKX09_05660"
     CDS             complement(20233..20931)
                     /locus_tag="PKX09_05660"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF015685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOU17343.1"
                     /translation="MHYLEINLDPFIELGRIVDIPLGNPLNLLGFIEAVGVAGISFTN
                     TPERKGMINITALRSMSRCRLNIRTNIENQAIQRAMSAKPDFITLINLDESDLTVDPH
                     SQIVQNLIETVQSAEDFNLILRLKPDIRDLKAAYRLKVDEAELATDTLVAQTLQAPYS
                     DELNKIVAASRLAIRNHMRVSLGGRLHKRLILSLLELIDVEFISVGRALLGQALITGF
                     DSALRELLEIVENR"
     gene            complement(20936..22096)
                     /locus_tag="PKX09_05665"
     CDS             complement(20936..22096)
                     /locus_tag="PKX09_05665"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOU17344.1"
                     /translation="MTRTSNYLKKPSKIVEKRHRDEKRLNALFSTVIGMQIILLVYFL
                     PKMAPSRANLFSEGKSRLINVARPFLIERGCQEIKSSSVATDRVSLIFELPASRDCEL
                     LAQQVSAYLNEHGFMVKKIDNLVKETGFTLFVDFQHIPIGTLAFIKTDRPSKAQMPLS
                     GVKPKLAIIIDDFGYTNREIVNNFLKLPAKLTISVIPGHPYSQWVARNAKLAGKEVII
                     HMPMEPEQSSNSHGEDKYMLLTGMTPEEISHRVEMACAELPEAVGMNNHMGSLFTSDP
                     ELMQIVINSLKRKGLYFIDSMTSAESVAYEVAIRNQVPAAIRTVFLDNVRDKGEIQAQ
                     FERAIEIASRGGRAVAIGHICSETLSVLTEILESGLLNEVELTFASEVTYLK"
     gene            complement(22205..23560)
                     /locus_tag="PKX09_05670"
     CDS             complement(22205..23560)
                     /locus_tag="PKX09_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU17345.1"
                     /translation="MDKIGTDVELKPPSARYRWLVFFFISMAMFGNYYIYDSISPLAD
                     LLKAQLGYSDANIGLLNAIYSFPNIIMVLIGGLIIDRIGTRKSVLIFTILVMLGAVIT
                     ALSGNLLLMATGRLIFGLGAESMIVAITTILARWFKGKELSFAFGLNLTFARLGSFLA
                     LNSPTWGRSLYTSWQKPLMVTAVAGMMALFFIGIYCLLDLLASRRLLLPQESKQDKIE
                     LREVFRFGAGFWYITALCVTFYSAMFPFQTFAIKYFQEAHGTSREVGGNLSSMLTLAA
                     MIFTPLFGLVADRTRRKAWLMALGSLLIIPVYLIMAYHIDLATPLGFHDIINIKFSFF
                     GINSSIPAYLIIPMAMMGIAFSLVPAVMWPMVAIIVDNNRLGTAYGLMTMIQNIGLFS
                     FNIIIGLANDFTGASADNPGGYNLGMWIFSLLGFCGLLFAFRLGKYEKSMHHEQIKEN
                     G"
     gene            23731..23898
                     /locus_tag="PKX09_05675"
     CDS             23731..23898
                     /locus_tag="PKX09_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU17346.1"
                     /translation="MAYVISEDCTACGTCIDECPAEAISEGDIYKIDPDLCTDCGACA
                     EVCPSEAIHPA"
     gene            24226..24912
                     /locus_tag="PKX09_05680"
     CDS             24226..24912
                     /locus_tag="PKX09_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17347.1"
                     /translation="MSSRRWRAGAGLTEIQRHIHWVADLPDIITVILSDGSLNELETL
                     FDNHKIILAPNNGTEIYAADFSWNLPNLNLIRQELTDIYCAVRESLGAKLQPEYLGFS
                     QSELWLKVSPGAEAVRKSLIEQFKLQNPAHLRLITQSNQTTIVPLIKWDRGQVIQKLM
                     DLIPHPLNQTPLVIYFGVEENDEAAFQYVNQVGFSVIIHNRIPRATEACYYLRNVAEL
                     SKFLFWVHSR"
     gene            complement(24977..25228)
                     /gene="rpmA"
                     /locus_tag="PKX09_05685"
     CDS             complement(24977..25228)
                     /gene="rpmA"
                     /locus_tag="PKX09_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOU17348.1"
                     /translation="MAHKKGVGSSRNGRDSRGQRLGVKRFGGEQVSAGTIIIRQHGTK
                     IHPGENVGIGSDDTLFALIDGKVKFSRKGRDKKIVSVLA"
     gene            complement(25234..25647)
                     /gene="rplU"
                     /locus_tag="PKX09_05690"
     CDS             complement(25234..25647)
                     /gene="rplU"
                     /locus_tag="PKX09_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOU17349.1"
                     /translation="MNNQNYAIAEIAGDQVILEPGKNVAVPKMDLEVGAKFLANKILF
                     IHTGNDIKVGKPYLEDFAIETTVLEHKRNSKVVIFKKKRRKGYRVKKGHRQPYTVLQV
                     SEFAKPEAAAPVKKSRAKTKTITESAEPAEQEKKE"
     gene            complement(25840..27678)
                     /locus_tag="PKX09_05695"
     CDS             complement(25840..27678)
                     /locus_tag="PKX09_05695"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU17350.1"
                     /translation="MNSPSAETRYRLCLAIANCRDTLLVTQLTTLLDDQSPTVRTGAA
                     FALGQLASQTAESLLVQHLYSETDAEVRQQTVLALAQIGDLSTLNFLLETHPELIGQA
                     LIYFFQRQIYNSASLNYAVSRLQSNQHYESYQAASTLSRVQNPTVMQEYVDILKTALK
                     TAGQATRVKIVTCLAPVNFSGKAEIWTQLLTDSVPEVRLEAARNIRIVAHDPLILHQV
                     FDDSFPQVVATAIENLPDSLELIPVLQDDIRILAHHPSRAVRSALVKFLSQCGGQETL
                     ANFGLWPVETNLLPAFAEGIAASGQISGLAVLDSLSRHQFRAISTPAYLGLINLAEQF
                     CRQDSIPCEQFCNYIAAGLKSDDPVKIAIAAVAMRDSTAKCVASEPLLYPSLNKFKNA
                     EYADAVIEILKTIAVRQPPDAVPFIQPLLNARDFRIRDLAYQILRETYEANVPEIISD
                     YRGAGRHSNLEMLKKYGLRPTILLETECGNIIIRLDGYYAPFTTDAFLSAIERGFYNG
                     LNFHRVVPNFVVQGGDPRGDGWGGPGYTLLTERSPIEFNCGAVGMARTEYDTEGSQFF
                     ITLTDQPQLNYQYTRFGEVICGMDVVKRLEAGDRILAISVVTMDER"
     gene            complement(27834..>29949)
                     /locus_tag="PKX09_05700"
     CDS             complement(27834..>29949)
                     /locus_tag="PKX09_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HOU17351.1"
                     /translation="NDSNWLPIRVPDYWENQGFEHYDGLAWYRYHFQADSALLRQDSL
                     ILYLGKIDAADETFLNGVCIGRSGAFPPESLSTKNNVRQYVFPAHLLKPDNLLAVRVY
                     DEGEKGGIYSGPLKIVTVESPKTPLPSGKVLQNSINIIPFTNGIASCCYNLKTRTFTN
                     FQPHIYRKFDEKSETPNLISQAKVVLFRNQEEIPLTNLVTLEVGYLEGTGIVRHILGG
                     DGFKLTIFGYCPFNLNKPFWVFYALLEGEKIDDLSLNFIYASENLDLDIGKWAYQESN
                     SKWLTVYIFYNPNPSADDADQLFLRKYKNEHPGWQALIEEIDWWQKWQVQTLLPENIQ
                     EDARKVYLQSLAVLKMAQCREPFPAGGQIIASLPPSTQNYCRPRDQAFAVEALLLSGH
                     SEEARLALQFIMNSRCGRYKIYQKKDQNQGIGVDYAVSVYRYLGNGTEESVSDENGLT
                     IHLDNFGLMIWNIYRYVEVTEDLKFLKYYWPKISRKMADVLPTLIDVSGLVRAEPGPW
                     EKSLPYKHYTYTSACTYRGLLDAARLARQMNDENRAQYYEETAINLRINIEKKLTDSQ
                     LKALKGNLEDSDPNLYADASSVEALNWIFNPQDQITRNTLELFKNYLTLANAQRGYCR
                     TRQQLITNSQEWVFGDLRIISAARKATRFEEAANLEAWVTRQALNNFGLLPQYFHIRS
                     ADYLGAVPRCGLGAGAYIINFW"
     assembly_gap    29950..30049
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(30310..31005)
                     /locus_tag="PKX09_05705"
     CDS             complement(30310..31005)
                     /locus_tag="PKX09_05705"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOU17352.1"
                     /translation="MTKQHLAKDKIIQLLDRSLDYKEESTAFEHWRTCPDCQAKIRTA
                     IDCAPPWLYNLPNHTNLWAIPVSARWQFWIIGSKEVLLALTFSEKTQRRQIELLRKQG
                     LELARSPAPIFLTNTAEMIYDFLETGQPYKVTALNFCLVSTAFARQVLTWTGLVPFGK
                     TTSYGRIAEWIGRPLAARSVGGALHSNPLGLVIPCHRVLGINGALVGFGGGLEMKQKL
                     LELEGIYPKMQTG"
     gene            31174..31878
                     /locus_tag="PKX09_05710"
     CDS             31174..31878
                     /locus_tag="PKX09_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU17353.1"
                     /translation="MTPLIEYDKKITQLIAAGRYDEALWVAEEELCYAESSYGHEHLE
                     TAKVLNNLGWICDVLNKEQSAEDYYLKALEVKIAVCGNTSHELIPTLENIINFLINHN
                     QLARAYEFLVKLIDLAGLQNKLWRLRKGAYLCQLAELEEQLGFSDKAENLLWESLSFL
                     QANYGFDHPNAGRVFAKLGAFFERQGNLARAEYCYNRSLKILKKHLSSNHPDVKFSRD
                     GLENIYSKLGLKVKIR"
     gene            31932..32735
                     /locus_tag="PKX09_05715"
     CDS             31932..32735
                     /locus_tag="PKX09_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOU17354.1"
                     /translation="MRIAIYQFAPIWGEINNNIENISRAIASQAADLWILPELCTTGY
                     VFSNVEELKPYAENVKEGATVKALCALSHQYQTALVFGMAERCGDQIFNSAVVLSKGE
                     ICAVYRKVHLFYEEKKLFSAGTETPPVIEIQGVKVGVMICFDWFFPEMARTLALSGAQ
                     IIAHPANLVLPYCQAAMLTRSLENRVFTATANRIGSERCGTKRLTFTGKSQITSPRGE
                     LLGQLKTGRPGILARDIDPTEALDKSLTPLNDIFEDRRPEIYQLGKSSR"
     gene            32732..33013
                     /locus_tag="PKX09_05720"
     CDS             32732..33013
                     /locus_tag="PKX09_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17355.1"
                     /translation="MMKSLPNPEELRLENQRMLWLRVYTDRVTHRLQTMSLDEPEAVS
                     LLEQVKQEVLQRFPDKERAYCLIYERRFKRILQRRGLFLPLTIDNGEMN"
     gene            33034..33594
                     /locus_tag="PKX09_05725"
     CDS             33034..33594
                     /locus_tag="PKX09_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOU17356.1"
                     /translation="MNYQSAYELFCRYTKTESLRKHALAVSAAMRHYARIFNEDEEYW
                     AITGLLHDFDYEMYPDEQNHPYRGNEILASEGYPEEMRTAIMGHASYTGVKRESLLAK
                     TLFAVDELSGFIIAVALVRPSKSIFEVEPRSVQKKFKDKAFAAKVNREEIKQGITELG
                     VDETEHIQNVIDALKSVAAELGLAGS"
     gene            33725..>34586
                     /locus_tag="PKX09_05730"
     CDS             33725..>34586
                     /locus_tag="PKX09_05730"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOU17357.1"
                     /translation="MRIILTQVEKPGRYVGNELNAIRKDHTRTAATIALAFPDLYDVG
                     MSYYGFQILYHILNRETDIAAERVYVPWPDMSRQMRSRQIKLFSLETHTSLDQFDVIG
                     FTLPHELTYANILTMLDLAQIPPKAINRKPSDPFIIGGGSGAYNPEPLAPFFDLFVIG
                     DAEEIVVPLVRLLAKGRRQSTPRQVILEEALHRFAGIYVPTFYKVENGRTIPATSFAP
                     GVIQANKIRTLQNGYYPQAPLMPLIEIAQDRLVIELMRGCTEGCRFCQAGMIYRPVRE
                     RQPADIIRQID"
BASE COUNT        10039 a         7402 c         7137 g         9908 t
ORIGIN      
        1 gaatcacaag ctttaaatta taccaggtga gcgccacact atttgttatg cttccacttt
       61 tagcttgatt ttttctctat tggcaaatat attcaaatgg tgataaaata ttttatttaa
      121 aatatatggg aaggaaagat gaaatttaaa attacgctcg tatttataat atgtttagtg
      181 ctgtcaggaa tgatgaattt cggtttagcc cagtctgatc aaccgctaat tttttctttt
      241 aaacaaaatc atacggttct ttataaaata gctaaatgct caaactgcga taatgatgaa
      301 aactttgatg taattggaat tgcaggcttg gtagatagcc atggccaatt agttccgcgc
      361 tctacttatc tggttcatct ggaagaatcg gaaaatagcg attttacagt ccagtggagc
      421 tataacttgc cgcaggatat taaaggtgat ttcaccgaca tagccgtaat taattggaac
      481 aatgtaccag ctattattgc ggtattaaat attaccgaag ccggcacggg tcctgatcca
      541 ggttggttat tacttttcga atattcggat ggattcagta aagaacctac agttcggatg
      601 gccaccgata ccgtcttccc tatcaggcct cgtcctagct atctcagtgt cggcgatctc
      661 gatcatgatg agcagtccga cttaattatc tcatcctcca gccccaatcg gagtattaat
      721 atagttactc aagccgccga tactagtgct ctttctctta agttaattaa ttattccaat
      781 gatctgcctg ttttgctcgg tcttcaacct tttcgggcat tagcggctaa tgttgatact
      841 gaaccaggtg atgaaatcgt cattttcggg ggacagcaga aattaacagt tgaggtttat
      901 cgctcagatt tgaagaatcc attatcttca tatacttttt ccaatgtgaa tcgtaacgac
      961 attaatctcg accaacttgc ggcgggagat ctggatggtg acggtttggc tgagttcatt
     1021 ctacctttac gtagcggggg agctcagctt ttagtaaaag gagtgcaaag ctacaaatca
     1081 ggtctattaa taccaaaaaa gaacaaaata gcggctctta atttggttga tattaacggc
     1141 aatggtctcg atgaaatttt attggctgat gctgccggtg ccgatattta tcgctatgaa
     1201 tataatccag gagcgaattt attcgatctt gatacctata tttcttttca ctactcaaat
     1261 ccggttttaa ctaatgtgca gttattatcc attgcgccgg tggtttctta tggaggcaag
     1321 aatacagggg caataattac cccgtttctt catcgcagtt ttaaccaaca tggattgtgt
     1381 tattggcttc tggaagagac aagtccgctg attgatgaga gcttaattga aacaattctg
     1441 ggatcggttg actcggtact ggctcataaa gaatttgatc agaaaaaggg tgcatcttcg
     1501 ctgactgaaa tggatttatt aaagcaacaa ttgggtagcc ttgttggtac agaaattgaa
     1561 ctttctccct taccggaagg taaaggtgga ccatcaggta tttcagccga ccggcaaatt
     1621 tatcagcctg atattctatt gcatcccggc gagttaatgc gttataattt aaatcttcca
     1681 gatttaagcc tcgatgatac taaaaaatta agtgtaaata tcgatgcgcc atcaggtatg
     1741 aaattcgacc tagccaataa attgttcact tgggttccgg ccgattccca attagggctt
     1801 cataaggtta ccgccttgat ttcatgggat gagaaaaaag atttgcagac ctttacggta
     1861 tatgtaaaca gtatccccaa aatattaacg aagatcccta tccgtgatat tatccagatt
     1921 ggtgaaacat tcagtctgca aattgaggtg gaggatgcaa atcacgacgc gatcattttt
     1981 tacaaattgc tggaaggtcc agcaggggcc agtatcaatc aaaatggagg attattgtgg
     2041 aagccctctt tcgatcaagc tgattggtat gattttatag ttgaaattag cgacggttac
     2101 gattcagaac aaaaatcttt tgccttattt gttaatcacc cggttagcat ccaatctacc
     2161 gcccctaaga caacaactat tgggaaaaaa tatacctatc agccggtcat tagcgataat
     2221 aataagggat tctatttatc cggttatttg cttccacccc ggatagagaa ctggagtcaa
     2281 actggtgtcc tcgaaactca gattcttgac aaaaatattc gcaataatat tagcaaatat
     2341 attgaaaatt acagaaaatc ttttccatta tttatcacac cggcaaaaat gaacgatgtc
     2401 ccccagagtc ttttccagga tatttttatt gatagcgaaa aggttgtgtt catttataat
     2461 cttcgaaccg ctaaaggtat ggaagcaggg caaatcattg aaacgttttt cagtaatttg
     2521 aagatgagca ttccaaaata ttctactccg gttcgtcgtt atctctatat ttttaatctt
     2581 aaagaaccga tcgccgggtt gacgatgaca ggcgagggat taatcacctg ggttccagcg
     2641 gctaatcaat ttgattatca attaatttcc tatacggtga ccgacggctt tttctcagca
     2701 gaggagcagg ctcaggttta cgtaaattac ccgccgaaaa ttacttccaa gcctgttact
     2761 attgcctctg ttaataccct ctggcagtac gaagtcaagg ttacagacct aaatacagat
     2821 agtaaattaa cttatgaatt gctcagcgct cccgaaggta tggtaatttc tcctcaggga
     2881 ataatttcct ggcagccaac cgatttacaa attaatgctc ataattttac gatcaaggtc
     2941 agtgacggat tcgctcagga tgttcaaaaa gtacacctat tcgtcaatgt caagccgaaa
     3001 atcatctctg tgcccaagcc ggtagcgcta actaatttaa aatatgaata taagcttgag
     3061 gcggaagacg ctaataatga taagctgact tatgtggcag tccgagtgcc taaatatgcc
     3121 agttttgatc cgaccaccgg tatgcttgtc tggacacccc gcaaagacca aataggggct
     3181 aacgatatcg tgctgaaagt cacagattca catggcggaa gtactctcca tgaatttcag
     3241 gtccatgtct ttgttaatcc ggcgcgacga cattcttttc tacgacggac gatatcgctc
     3301 ttgactattg ccggtgtgat ttacttagtc gtaatttatc tataaatccg ctccggaaat
     3361 agatgaaggc ggtaatcttt gcaagatagc cgtttttatt tcatcagata attttagttc
     3421 aatttcgata accaaaagcg gaatagccaa tgatccagca tttaccggca ggttgacagc
     3481 gtctttttca gtcgtaacca gatagtcaca gttcatctgt tcggctttga ttattatctt
     3541 ctgcaaatcg gtggtcgtat aatgatggtg atcgggaaat cgtaggaatg tcaccttacc
     3601 acagtttaat tgtcggacgg ttgtctcgaa ctgaatatgg ttgccgagcc cacaaaacgc
     3661 cagtacattt ttaccagcga tagtattgat tgagacgttt tctgcggaaa gggtgcgaag
     3721 accaacgatt tttagagaag ccgtaaaaaa tgttatgctc ggattaaatt tctcgaagaa
     3781 ggttcgttgt tcggggatga ttttatccgt cttagaaaaa actaaatagt cggcgcgttt
     3841 taatccgtgc caggattctc taagccgacc gaccggaaaa aggtactttt gccaggacgg
     3901 tgttttcgga tcccaaagta cgatattaat gtcacgggcg agtcgccgat gctggaatgc
     3961 atcatcggca ataatcaaat tgcaagcaaa attttcgatt gcggtatggg cggcggctat
     4021 acgatcagca tcggcaacta tgacaactcc gggcaattta ccagccatca aaaaaggttc
     4081 atcgccagcc tgttgtggag tagttaaaat ttgtctgcca tcggaaacaa taactgtacc
     4141 cctggattta cgtcgatacc ctcgagagat gatggcaatt cggtaacctt tttcacttag
     4201 aaattttgcc agcgcaatta caaacggtgt tttgccagtt ccaccagtag taatattgcc
     4261 cacggcgacc actggaacag gcattttgat agctttaaaa atatgacggt cgtaaaacag
     4321 attccgcagt tttgtgatca aaccgtatat aacagccgct ggaaataaaa gccatcttaa
     4381 ggcacctaat tgcattctct attaatgtat ttatgattct aaataattca ggataatgtg
     4441 agccgcacgt cgggaagcac ctggttcgcc taacaggcgg cagacatatt gcaaaaaatc
     4501 gcggatttgg ttcgcatatg attgatcgca cagaattctc atgatttcac ttttgatgtt
     4561 ttctacatta gcatcgtgct gaatcaattc cgttacaccc tttttcccgg cgatcaaatt
     4621 ggtaatcgaa ataaagggca ccttgattag ttttttcccc agccagtaag tcagcggtgc
     4681 cacgcggtaa attacgacga aaggcttgtt actaagcgtg gcttcgaggg taactgtacc
     4741 ggaagccata attaggacgt cggcagattg aatcacagaa cgggtactat tgaaatgcca
     4801 gcttagccaa tcgaactgat agcatttttg gtatttttcc agattgacgt cggaagcaac
     4861 cgcaacagcc gcctgtaaat ttggaaattc ctggcgcaga tctttgaccg atggaatcat
     4921 tagcggtaga tgcttatcaa cttcttgctg acgactgccc ggaaacaacc cgaccagcgg
     4981 cttgccgctt ttaaaaattt tctgatttga ttccggtata gaaatttcat tctggtctaa
     5041 taaaggatgt cccacaaatg ttgcccgcac tcctcgttcg ctaaaccagg tggcttcaaa
     5101 tggtaaaata cagataactt cctgaacgtg gcggcgaatc ttcttaaccc gggattgatg
     5161 ccaagcccaa acttgaggta cgatataata aatcactggt atatcacgcc ggtcgagcgc
     5221 cttagccaag cgtaagttaa aaccgggata gtcaattaaa acgacgacat cgggtttaaa
     5281 ttcatcaaga aactgcaacg tcttacgata catactgatg ataaatggta gatgtttaat
     5341 aacctcaaca aaacccataa aagacgtttc gcgaatatgg aaaagcagtt cagcgccagc
     5401 cgccgccata ttatctccac cgatggcgct gatttcgata tcaggattta attctttcag
     5461 ggcggaaatt agtttgccag catgcatatc gccagatagt tcaccggcag agataaaaag
     5521 tttaccagcg gacatttagc cactctttga ggattacagc taaagcgaaa attacaaaaa
     5581 ctaacagcgt cgatctttcg gtacgtagta atttccgatt gctcagaaat tttggctgaa
     5641 ctccactatt ccagggtttt agacgcggcc ataagcgcgg gacattggct ttatattttg
     5701 catattccgc accaaaaata ttagtcagcg tttcttcttc gagagatata attaagccat
     5761 actgaaataa aatgtaaacg aatactaata acatcaaagg cagaagccag cgcccacccg
     5821 cgaaaaacac cattccgata taaatcaaag cattaccgat ataaagcgga ttgcgtgtat
     5881 gggcaaaaat gccccaggtg caaagttctt tggcgcccac attgcgagta cgggtacgac
     5941 cgccagccgc ccgtacccca ttcagtcgga tatattcacc gaccaaagcc aaaattgctc
     6001 cgataagagc taatttaaag ttcatatcag actggtaaat tagaactaac ataagtggaa
     6061 taggagtgta accgcgtatt ttgaaaaaaa gacgacgaat atccattaat tgattttcct
     6121 aaaattatct tgaatagaat ttataatctg gatggcaact ttcagagcgc gtattccatc
     6181 ttcaccacta acgcgcggcg gttgaccagt tagaattgca ttgacgaagg actccaattc
     6241 attataaagc gcgttaccct gttcggtttg ttggacgcgg tagaaaatcc gtcggggggc
     6301 accgttcaca tccggcactt ccataatttg agtagtatca tcaggcaccg gtcctgcatc
     6361 gcgagataaa gaatagattt cggtggaacc ggctaacata tcaatcgaga aataagcatc
     6421 ccgctggaaa attcgcaact tgcgcatttg tttgttcgat acgcggctgg cagtaacgtt
     6481 agccacacaa ccgttggcaa agcacaaacg agcattggca atatcaatat tatcggtcag
     6541 aataggcgcg ccggaggctt ccacctgaac tatcggtgcg gtgactaaat ccagcaccag
     6601 atcaatatcg tgaatcataa gttccagaac aaccgcgacg tccgtaccgc gaccacgaaa
     6661 aggtgcaatc cggtgtgact cgatgaacaa cggatctatt tgatactgct tcaatgcgcc
     6721 caaagccgga ttaaaacgct caacatgtcc aacctgtagg acgagatttt tcgcgcgagc
     6781 aagctggata atttcctcag cttccgcaac tgtctccata aaaggttttt cgcaaagtaa
     6841 gtgtcgtccg cagtcaatag cggcttttgc cacgctataa tgggaatgag tcgggaccac
     6901 gatcgaaacc gcctcgcaat cgtccagcaa ttgttccagc gctggataag ctcgaacctg
     6961 aaattcgttt gatatatagg atgcccgctc agggttgttg tcaaagaagc cgactaattc
     7021 ggcattgggc aaattttgta aatttttaat atgccagcgc ccaaagttgc cagcacctac
     7081 tacaccgatt ttaagcataa tatatagaca ttctcctaaa ttgttctgta tcgaataaat
     7141 tttaaaaaac ttgattttgc ccgtcaatat aatgaaattc ttgattatgg aaaaggtatg
     7201 tttcattcaa aaaaactggc taagccaaaa gttcgcaaat ggaatatctt tattgattct
     7261 aattatggtg atcaatttat gggcggagct gatcgccgaa gtcccccaag ttgtcattgt
     7321 ccattggaat gatttccaca gtactaacct tccttataaa tccagtcagg cagactatcg
     7381 agtaggtggt tacgctaatc tagctggata cttcgattca cttcgccatc ttcatcccca
     7441 ggcgatcatt ctgaacgctg gcgatgattt tcaaggctcg ccagtttcga atttaactcg
     7501 gggtctatcg caaattttga tcttgaataa agtccaacca actgctttca cactcggcaa
     7561 tcatgaattc gattatggta ttgataatct tcgaaatgtg atgacctatg ccaacttccc
     7621 gatcctgaat tctaatctat ttgactcaac gaaaaacgat ttattagtcc aaccttatac
     7681 tatcattcaa tctgggaatt taaaagtcgc tattattggt gctctaactg gagatttgtt
     7741 caccctggct ttgccgcaaa atctcaaagg catcggtatc cttgacccgt tgacccaaat
     7801 ccgccaatat gtgaacgaag tcgaacctca aacggattta atcgttttgc tgagccattg
     7861 cggttttgaa gaagattcgc ttttggctat gcaacttgcc ggtgtggatg ttattattgg
     7921 cggacattcc catacctact tgcaccagcc taaaaaggtc aacaatatct tgatttgcca
     7981 agccgggtcc aatggtcatt gtctgggtta tttgaaagcc aatatagatc gaaatcagaa
     8041 aatcatcacg tcctataatt acgaattaat agaaacccgt ctcgggagag taaaaccgtc
     8101 accaattgtc gccggagtag ttgactccct tgaggcttct atctctgcag aaatggataa
     8161 aatcattgga caattgataa ccgactggca ccgcaatagc cacggtgaat cgaatctcgg
     8221 caattggatt tgcgatgtaa ctcgtgaata ttttaagact gatatcgcct ttatgaatag
     8281 tggcggcatc cgtaaagact tgaaggcggg accaattcga gtgcgtgatc tctgggaaat
     8341 ttcaccattc gataacacga tcgtaatagt cgcattgacc ggagaacaat tgaagttcat
     8401 cattcagtat cacgtcgaaa acccgcgtga ccttttacag gtttctggac tttcttatga
     8461 atttaatagt aaacaaaaaa agttagtaaa attgaccgtt gcgggcaaac cggtcgacga
     8521 tcaaaaaatt tatactttcg ctacgaataa tttcgtgatt agccaattcg aacgtttctt
     8581 cggtctagcg ccttccgcgg taaaaattac tcctaccgat atcatcgggc gtgacattct
     8641 aatcgaagcc atcaaagctc aaccggttat taataatcac acggaaggac ggattattga
     8701 cttagcaaat aaaaaagaag aggtaaatta tgacatccag tagtcgcatt ttgtatagtt
     8761 acataatttt gggaatcgcc ctagtggctt ttggtatcgc aaccatactt ggtcactttt
     8821 atatcggctt aatttgctta atgctcggaa tgattatatg gcttatcgtg acatttgctg
     8881 gcaatactga ggggcagagc ggtcaaattg gcgtggtaat gattacagcc ggatttatcg
     8941 gtggattaat aatttttatt ggaattggat tgcgtccgag cattttcggc ggacataaat
     9001 tatatattga aggaattgta acggcattag tggtattact gatttttatg gcaatcggat
     9061 taggttttat cagtctggcg aatcttgaga aaaaaatggc ttttttcaat aaagaattga
     9121 agaaattcta caagttgtat atgaaagaag ataacgaata aaaaagcatc cataatactg
     9181 gtctttactc taccttgttt ggcactgacg caaaatctgc aactccatta tgattttggt
     9241 cgtcaacatt taacctcgac ttacgagcta tataaaaccg accgactggg tgcgaccttt
     9301 ctctttgttg atttcgatta taataatgaa ggttcgtccg gttcaagaaa tgcttctctg
     9361 gcttacggtg agatagctcg ctatttccaa ctgccgaaaa tgaatgatct ttcggctacg
     9421 ttacaatata atgatggctt gacaaatagc ggttctttca attcggtttg gcttggcggg
     9481 ttacaatacg tttttcaaat tggaacgcaa aattttccaa ttgactttct tctgcgtaaa
     9541 gaactaaata ccgacggatt aacctttcag ttgacttatg tttggagtta tatctggcgc
     9601 aaattggaac ttagtggatt catagacatc tggaataccg gagcagatgc ctatccgcct
     9661 cgaaaaattg tcattttatc cgaaccccag ttttggtaca ggctcacccc ggcactttcc
     9721 gttggaggcg agatcgaaat tagttttaac ttctcaggtg cctggcatac taatcaggtt
     9781 ttttccgagg atgtaatatt tattctaccg acacttggct tgaagtggct gttctgagtc
     9841 cgctttcccc aaaaatcatc agattaagaa aaaaatcgtt cggcgattta aaataaagca
     9901 cccaattttt agtcgctcgg tctgggatat ttacttaaca atatctgaaa attgtcgagg
     9961 aacaattatt cagttgccgg ttcttcagcg acggattccg atttactttt tttctggata
    10021 ataagtagaa cagccagaat taacagacct atgactgtga ttacagccag gattttgaaa
    10081 attagtttta agtatttcat atagcaccca tagaattata attttttaag taaatcagcc
    10141 gcttctttct gcataacttt atcagaatcg gattgcgccg gaatttctaa agccaattgg
    10201 agattctttt tagcctgatc tttcttcttg agttttagtt gagtcttacc gagccagagt
    10261 agatgacgaa tttcctttgg cttaagttca tgtgctttct gaaaatactg ttcggcttct
    10321 tcgaaggagg ctttgggcgg cgttgcataa atcaggctgg caacctggcg ttcagcccaa
    10381 cttaaatcgg ctaattcaaa atgccagcgt cccataacat ggtaattcga atcctctttc
    10441 ggatctaatg caatggcttt ttcagtatgt tcacgcacgg cataggagtt ggtgatcttt
    10501 tgtttggtgc cttccagttc gccgatttgt ccgattaaaa tggcgtagta ctgatgtccg
    10561 cctgctactt ccggcgccag ctctacgcat tttttggcat actcaaagcc ggggtagaga
    10621 ttggcttttt ggacttcgct tacgccgggc tgttgatcag cgatatcaaa ataagctctg
    10681 gcgagtctcc atagtacttc tgggttatta ggttcaattt tttcggcttc gcgcaatttg
    10741 gtcatcgtgc cctgataatc tctggcatcg tgcaatttat ctgcttgttc gagtattgat
    10801 tttactttag ttgactgagg agtcagataa atcggtaaga cacataatcc cagaacaatg
    10861 aaaaacactc tggttgcatt agagaaaaat aattttcgca taggaacctc tgtttatttt
    10921 cttgatgaga aagattatga ccgttgttta aaatgtatag atgaggcgat atctccggga
    10981 ggctaaaata tttcagtaaa agtgaatttc aattcacgat tcataaagtc cacgtctttg
    11041 cggttcagca ggatattatt gaaatctagt tcaacagtga acttatctac caatctccag
    11101 cgaacaccgg catttaaata gccccaccct ttaccgagca gcggtaattc cgtgtgattg
    11161 tcgtttagag ccgcatcata ttcgataatc aaagcaattt cggagttcag gtctttatct
    11221 atacccagga aaaaattggg gtcggcatcg ccatccgttt tctcgatcgg attataatta
    11281 attccgccat gaagacctaa attgcctaga aaattgaaat ttttgctgac gacaacatag
    11341 aatccagagg ctttggtttc atagcgcttc agtgaatcaa tataatttcc atgaccttgg
    11401 gacgaaaaac caaccagtaa ggccggaaat ttaacgctct cttcgataaa gcgatatttg
    11461 atctcaacgc ccggctgatt gtaccaatta atcttctggt taccaataat cccaccaccg
    11521 ccgtaagata ctccgaacat aaatctttcg aaaactccta cgcttatctt acccgctaat
    11581 cctccgcctg ggaaaagatg acaatcaacc gcataggaac cacgcggcaa tataccagca
    11641 gtcggtatgg tgaccagatt aaccggctga tgaattccta aatcaccttg agccgataga
    11701 ctgccagcca gcaccataaa aaagacaaag gggaaaaatt gttttctatt cataaccaac
    11761 tctcattttt tgttgaatat acattcggca aagctgaaaa tcaaatgaga tttatagcct
    11821 cgtcgataaa attcagataa atgatatata tcaaacaaat taagtttgca tttgatagtt
    11881 tgggctaaac tttttatatt taaacgtttt taagtattct atgcagtttg ggacaaaaaa
    11941 agtgaaaagc atccagaaac taattcagaa agaaattgcc aatcgaggaa gtcttattgt
    12001 cccctttaat aacgaagcat tactgagcat tttatttgtg gcattaacat acatattggt
    12061 ttaaaaagtg attttcaatt taaaaacaca tagtatcact attgaaatta tgacctttga
    12121 atctcggcag gaaggggtat ttttatgaaa ggttttggcg ctgttcttgt tgcgattagc
    12181 ttaattttgt tagtgctcga gcatttgaac ttagttgatg tagtcggtat tatcggtttg
    12241 ctggtcggtc tttatgcgat aattttccct gataaagccg aagaattata taccaagtta
    12301 aagtcaggca aactgaagta accggcctca gatcgctatt ttttaaaaat agtgtaattc
    12361 ttaatctatg aagccagaga tgtaaaattt aattgcttgt ctatttagcc aaatcaatta
    12421 aatatttgat taataatttt agctaaaatg ccaatttacg agtttgaatg tcagaattgt
    12481 gggagggttt tcgaggaatt agtcttctta aaaaactttt cagaaagcga aattacatgt
    12541 ccgcattgcg gatttaagca agcgaaaaaa ttgatctcgg caccggcgct aagtagatca
    12601 tccacttgct cttcttgcgg cgctaaatcc tgttccagta gttgtggttc tcgaagccat
    12661 tgattcatta cggcaattgc cggaactcat cttttgacga accaataaaa taaaatttat
    12721 agataaaatc gaggatttat tcagcttgaa atgaaaagta gcaaattgag tggaaaattg
    12781 tcttcagtag tttaatttcc ccatataagc attaaaaact aactcagtta atcactatta
    12841 tttgtatttt tatatagact tttagcgtgc tcggctgaaa cagtaatacc ttttatcatt
    12901 gctgaactaa aaccctcgtg ttccatctga ttcaagccgg caatcgtaca gccacgagga
    12961 gtcgtgaccc ggtcgatttc atattcggga tggttggcat gggttagaat caaagtcgcc
    13021 gctccgcggg cagtctgggc ggcgatatca atcgcctcct gggagtgaaa accaatctcg
    13081 atgccgcctt gagaagccgc ccgaatggct cgtaggaaaa aggcgattcc acaagctccc
    13141 aaagcagttg cggcagtcat tttttcctct tcgatgacta tagttttgcc caccgtatcg
    13201 aaaatagatt tggtaattgc gatttcggct tccgaagcgg tatccgatgc cagacaggtc
    13261 attgattccc gtacggctat tgcagtattg ggcattgcgc gcacgactgc gatgccagat
    13321 ccgacctttt tcacaatttg agaagtattg actcccgtca cgactgatat gacgatatgg
    13381 tggttgggtt ttaaatgggg agcgatttca atcagaaccg aattgacctg ctgtggttca
    13441 acggcaatga taataatttt tgcttgacga acggcctggc agttatcttt ttcgataata
    13501 aaaccacgtt tttgcatatc atccagcaag tgaatgcgac gtcgagttaa aataatctcc
    13561 gcaggcttga aacggttaga gtcggccaaa ccattggcta ttgcagtccc aatatttcca
    13621 gcaccaatga ttgcaatttg attgattttc atagtgaatt ctcctttagc ttcaaacgga
    13681 tgcccctgac cagtcgtata tttcacggtt aatccgccga tggataaacg ctgtctgcct
    13741 taataccttt aacttggtat ttggtaatcg gtaatccgta agtagttctt gataaatctg
    13801 catatttaca gtataaattt tcacaacact aaattttatg ataataaaaa gtcattgtca
    13861 agtagccgga ataaatcaga ttctcacatt gagtcctttt ctcatagtta cattatcggt
    13921 aaacaacatc aggtttaact ctccattttg actattaaat ttcatctaat gacgcggatt
    13981 gccgagtttt agcgagagaa ctcggagaat atccaccggt gataacattg ccagccgctt
    14041 gcgcgcctct ttaatatcaa aatgatctac ttcgcgcact tcacgaatca acggtaacat
    14101 aacagctgag acaagcagac ggatcaaagc actgagcatt aaaaccagtg ctaacgccga
    14161 aaagcggtga aattggaacg gtaagtcagc aatcaacccg ccgatcaaac caccgagaaa
    14221 cattccaatg ccattaaaaa tgttgtaata agccacacaa atcgccatcc gttggcgagt
    14281 aaccgcatcg taaatgaaat tgacggttgt gagattgaat ccagcccagg caaatccact
    14341 cagggtttcg actaagatca agtacagcac cgctaaaaca ggacgatcaa taaaaaatac
    14401 tgccaaaacc cataatgccg gaatgatcgg gaggcaaaaa ccggtgaggc gcacgacccg
    14461 caaattgccg aagcgatcgg caaagcgtcc ccaagcgggc attagaagca tattgacgaa
    14521 cggcatagtc atagtaataa gagtgaactg aaaataattg aagtgtaaat cctccagtat
    14581 aaatactgca aaaaacggcg aagaaatata tacggcaaat tgcatcaggg caacgtaagt
    14641 aacaaattta ccgaaattgc tctggggaat cttgcgaatg aactgccaaa tcgtgaaata
    14701 atattccttc tgaggatgaa attccggttc gtattgacgt tttaataaag ctgacgaaag
    14761 agcgcgtccg gtaaaagcaa tcagaaaaat gatcgcaaag cccgtaaagg gttgccccaa
    14821 cctgttgaaa tgttgcagga ttaagccagc actaagcata gcgagaatag tggtaaatcc
    14881 tacaacccga ttgcgaaggc caaaataagt cccgatttgg cgcgggatga tgtcgcccat
    14941 ccaggaactc caggctgggc caacaatacc gccgaaaatt accatcagac tgtaaaaaac
    15001 cattagtcca gtagaaatca ccatattgtt tagattataa ttaaaatgca accaacccag
    15061 taaaataatc gggaaccaca ttaaggtttg caaagaagta cccacccagg caatggttct
    15121 acggtgaata cctttctcta aaaatttcaa cgtcaagagt tggatcaagg tccccagcat
    15181 ctgtggtaaa gaaatcaaaa ttccaattag agtattgctc attccgagca aaagggcata
    15241 cggagtaatg taacgcaagc caaatccgtc gaacaaacca tacgcggcgc cctcacggat
    15301 gctgatccga ctggcttttt tcttaagata ttcaatttta cgagcgtggt ttccagtcat
    15361 cgggcgcaaa tttatttagt agccaaattg tatccaagtc acttgtaaac aataaaaatt
    15421 tatacgtcat atccctaaaa tggtctattg atagacagat ttttcctttg ataatcgttg
    15481 gtatattggt aattttgcac gctttttgtc agggtgcata gctcagtcgg tagagcaacg
    15541 gccttttaag ccgtgggccg caggttcgat ccctgctgca ctcaccaatc caaatttatc
    15601 attgatatct aaatttacga gtaagtcccc tgtttgcctt attttccaag tggtacccaa
    15661 atccggcacc gagatgcaag cctcataaca ggttgtcaat aattcgaacc attttatggg
    15721 catcttttcc cgacgattca attcaacttt gggtggtggt gttaaaatag taaaaccaat
    15781 ataatccgaa agggaaaggt tttgacatta ctggctttag aatactcagg tgaaccattc
    15841 aaagctttta ccacttaaaa tcaaatagaa ttggctctga attcaaggac gaacaatttt
    15901 gcaggtcggt ctaaattcca ggttttagcg accgataacc ctgttcggta ctcactccat
    15961 cagattgttt aattcagacc gtcactaaat tatataagat gagagccggg taaacgtctt
    16021 cgccctgtct tgtatatttt taatttccta ccgaaataca gttatccaat tttagagagt
    16081 atgttagtga tttctgtata attgtcagca taaaaatgta atttcggcat atcacgatcg
    16141 ttccggtcgc accgaaaacc gatagttacc gctcccgaag cgattgcggc ttgaatacca
    16201 gtcattgaat cctcaattat tacgcactct tccggttgaa ctcccagact ttcgaacgct
    16261 ttcagatatg gttcgggatg aggcttagcc tggcgtacat catcggccgt aataattaaa
    16321 tcaaagatat tgcgcaccgg tgtgttacga aatacccact ccaaaaaact ggctgaggtc
    16381 gaagtcacca gcccagtgcg ataacggccg tcaatacatt gccagaagtc gaggaaaccg
    16441 ggcaaataat caactccttt gttgaattct tccttgatat aacgccgccc gtcttcgatt
    16501 aatttatgag catcagtttt aatctgatac ttagccatta tcaattgata aaaatcaccc
    16561 tcggttgtcc ccttaaaata tttccaatct gcgtccggaa cttttatccc atactggtca
    16621 aataagcgtt gttctgcgac caaatataag ggttcggagt tgataactac tccatcaaag
    16681 tcaaatatta ccgccctaat tgccatcctg ttttcatcct aatcgtaaat tactacatta
    16741 actatcattt tatattctgt actgttaaaa tcacaagtaa tacgccaaaa cataaattat
    16801 tatttctaac aaattctcct gattgtccca taattacaag ttactattgc taaaaattaa
    16861 ttattgctct cgatcggaag ttccactcgt atagttgagc cgctctccgg attattgaaa
    16921 gcagaaaatt tggctttata gctactcaat agcatataag cattgtataa attatactta
    16981 atgagagcct tctggtcgga agagtcttcg ttttcaggaa tctcacgata aaaactagga
    17041 gtaaaaagtt cctcggcatt atccggaata ataggctgtc ccgtggtagc aatttctacg
    17101 taaatattat cctgggatat accagtttcg attttcaata tttttcgagg agaatcgcgc
    17161 attgattcaa cacagcaatg gatgatattc atcaaactct gcgaaaagtc actataatgg
    17221 ccagtaatca gcggcagatc gcgtcttaag acgatttcct tttcaatctg ggttttaaag
    17281 aaagattcag tttcaagaaa taacagttca tttctaagca attcacttaa attgatcgtg
    17341 attttcttgt tctccagttc gaggttcatt ttatctatga aactggtcaa tagagattct
    17401 atttttcgta cgttgtccac aatgatgtcc ggctctttta actccggcag acgggttttg
    17461 agcatctggg cccgtcccat taccaccgtg agcgtatttt taatattatg gataatgcca
    17521 ccgatcatta aggtcagcga cattaaccgt tgttgcttaa gccattttac ttccagagct
    17581 tgtttagcca ggctaaggcg tttatattcg gaaatgtcac tgattacggc gaaagtagtt
    17641 aatttacccc gatactcgat ttccaccgga ctgacttcca cttcaatcgt atgaccatcc
    17701 ctacatttaa aggaagtttc gtatcgcttg ggaacggttt taccgattgc ctgccgacgt
    17761 tcgcgctcct tgagtatttc gactccgcgg ttcgtcaaaa tattatgaaa ctccataccg
    17821 gttagctcgt caaccgtata accaagcatt tcggctagtc tattattaac aaatataaag
    17881 cgctcttgat ggcagatagc aataccttca ttgatcttgt caataaataa atgatagagg
    17941 gacatagttt cagccatctt ctcttcagtt ttctttcggt gaagggcaga ggtaatgata
    18001 ctggcaacaa ttttcagcaa ttctatttca ctatccagcc accgccggga agtttttgat
    18061 accgcgacta ttagtaatcc gaaactggta tcgttatata ccagcggtgc aatcattaaa
    18121 gatttgattt ctaattgctg taattcctgc ttttctcggt tggcttttgc cggtagatta
    18181 ttaacgtcat taattatgac agtacctttt aaactcaact gatttttcaa ccagggggaa
    18241 tttgctaatg caatattttg caaaagttta acggaatcct ctgatttatc ttcgagccca
    18301 tatattttca gcgcctcttt cgtgtccggt aataatgccg ataccaaaca atattcggtc
    18361 tgataaaatt cgcccagatc tcgtagggat tcatttattc tgttgtctat ttcaactctg
    18421 ggtacgtcaa taaatcggag tgcgatttta attattaaat cttttttact aatcatattg
    18481 cgctcacttt tcattctgca gggaaggtat tagattttat taaaatccgc aaataggata
    18541 aatattgaat atatttgctt ctgatactat tgatgttcct atttaaaact atactggctt
    18601 agaatatttt caagatcact cgcagaaata aattggtggg ctttaaggcc gagtcgctcg
    18661 gcgccagcga cattttcctt aagatcatca ataaataggg cttgttccgc ggttacatcg
    18721 gcttttcgca aagcatattg ataaatcgcc gattccggtt tggtcgcccc gacctcaaat
    18781 gataaaatca atccatccat attctccaaa atattgaatt tccggcggat cgcccgaata
    18841 tggagggcat tggtattgga taaaagaaag agcggtactt gccgacggag ttttaaagca
    18901 atctgccaga cgtccgtatt caactcgaag ggagtctgcc aaatccgttc gaggtcatca
    18961 acaccgatgc tgtgatctat gtggtaaata tttttgactt ctactaaata ttctgcaatg
    19021 gttaattgac cacgctcaaa tttcttctca agtgcgagaa ttggcgcttg catcaccgct
    19081 ttttccggaa tacggaatag ttccgcaatt tggggaatag cagattcgag attgactttg
    19141 accagaacat taccgagatc aaaaaaaacc gctttaatta ggttgtttgt gcgtgacatt
    19201 ggttatataa ttagcgtctt aagtaatttc cagctattcg gcaactactt ccgtgaaaaa
    19261 tttgccgatt tcgacgtcgg tataactttt tactgccgaa ctgagataga aatccagttt
    19321 tttggcaatt tcatcatcta tcaagtgctt atgctttagg actgcgaccg ccatcgaatc
    19381 acaagcttcc cggttaccac tcagcacctt tatagcaata ccgatcggat tattttcatt
    19441 attccgcaga ccaattccac gcacaccttc cataccgaac ttagaaatac cgcgccctcc
    19501 cattaaagcc ccaaaatcgg tatcaaattg ggcacggcct ccgattagtt tcgggtggag
    19561 ggaaatgcta tggaaaaccc gtcgtaagta atcgtcgctc ccctcgacta attttttata
    19621 cattattgcc agattataaa ttggcattag ataagtcggg gcattactgt tatcagttgc
    19681 gaccggaata cgctctttgc cggagtatag ttttattttt tcaaagattt tttgctgggc
    19741 gggatggttg atctcggtat aattggacaa atcgtacttt aaagccttgg ctatcgcgag
    19801 cattccggcg tgagcccctg aattggcgtt gtgaagcgca gtaggtcttc gtccttgaat
    19861 agctaattgt tcataagttt ggcgatcgag gggcggatgc actccacata gtaaattatc
    19921 tatattgagg tccatttttt tcaggatgga attgagcacg tcaagatgat aatgctcacc
    19981 ggtgtgagtt ccgcacatta aagccagttc ctcatcgtta aaattaaatt gctccagcgc
    20041 tcccgattcc aacatagcga ccgcttgaaa aggattagca gcggctccaa tgaaaattgg
    20101 atattcagga tcccctgccg caaataggat ctcgccattt tcatctacga caacagcata
    20161 accaatatga aagctatcaa cagcatttcc tcttactaac ttacttacca gtaacatacg
    20221 aaaacatctc ctttatctat tttcaacaat ttcaagtaat tctcttaaag ctgaatcaaa
    20281 cccggtgatc aatgcctgac ccagtaatgc ccgacccaca gaaataaact ccacatctat
    20341 caattccaaa agacttaaaa tcaggcgctt atgcaatcgt cctccgagtg aaactctcat
    20401 atgattgcga attgccaaac gcgaagccgc aacgatttta ttcagctcgt cactgtaggg
    20461 cgcctgtaac gtctgtgcga ccaatgtatc cgtagctagt tcagcttcgt caactttcaa
    20521 acgataagcc gcttttaaat cgcgtatatc cggtttcaat cgtaagatta aattgaaatc
    20581 ttcggcactc tgcaccgttt ctatgagatt ttgtactatt tgagaatgag ggtcaaccgt
    20641 caaatccgat tcgtctaaat taataagggt gataaaatct ggtttggcag acatagctcg
    20701 ctgaatagct tgattctcaa tatttgtccg gatattcagc cgacagcggc tcatagaacg
    20761 cagtgctgta atgttaatca ttccttttcg ttcaggtgta ttcgtgaaac tgattccggc
    20821 cacaccgaca gcttctataa aacctaataa atttagcgga ttacccagcg gaatatccac
    20881 tatccgacca agttctataa aggggtcaag atttatttct aaataatgca tattgttatt
    20941 tcaaataagt cacttctgaa gcaaatgtaa gttcaacttc gtttaaaagg ccggattcga
    21001 gaatctcggt caggactgac aaagtttcgg aacatatatg gccaatcgcc acagcacgcc
    21061 ctccgcggct ggcaatctca atcgcccttt caaattgcgc ctgaatttcg cctttatccc
    21121 taacattatc aagaaatact gtccgaattg ctgccgggac ttgattccga atggcaactt
    21181 cataagcgac actttcggcc gaagtcatac tatcaataaa atacaatccc ttgcgtttca
    21241 gactattaat cacgatttgc atcagttctg gatcactcgt aaatagcgaa cccatatgat
    21301 tattcatacc cacagcttcc ggtaattcgg cgcaagccat ttcaactcgg tgactgattt
    21361 cctccggggt cataccggtt aagagcatat acttatcttc gccgtgacta ttagaacttt
    21421 gctctggctc cattggcatg tgaataatca cttctttacc cgctagctta gcgtttcgcg
    21481 ccacccattg tgaataaggg tgaccgggaa tgacggaaat cgtcaattta gccggcaact
    21541 ttaaaaaatt gtttactatc tctcggttag tgtagccaaa atcatcaata ataatcgcca
    21601 atttgggttt tactcccgag agaggcattt gggctttaga aggtctatcc gtcttgatga
    21661 acgccagagt tccgatcgga atatgttgaa aatcaacgaa aagggtaaat ccagtttcct
    21721 taacgaggtt gtctattttt tttaccataa aaccgtgttc attaagataa gcactaactt
    21781 gttgagcaag tagttcacaa tccctactgg ctggtaattc aaatatgaga gacactctat
    21841 cggtagcaac tgaggaactt ttaatttcct gacaacctcg ttctatcaaa aaggggcgag
    21901 caacatttat tagcctactc ttaccttcag aaaaaaggtt agcccgagaa ggagccattt
    21961 tcggtaaaaa atagacaagc aaaattatct gcataccaat gacggtagaa aacagtgcat
    22021 tcagtctctt ttcgtctcgg tgacgttttt cgactatttt agatggtttt tttaaataat
    22081 tgctcgttcg ggtcatagta ttttaactgc ataaaactac taaattccga ctgattctca
    22141 aaattgtgct acaaaaaatt atccacttcc tttagtggca aactacctaa cggttcaagg
    22201 ctgttcaacc gttctccttt atttgctcat gatgcattga cttttcatat ttacccaacc
    22261 gaaaagcaaa tagcaatccg caaaatccaa gtagcgaaaa aatccacatt ccgagattat
    22321 aaccacccgg attatctgcg ctggcaccag taaaatcatt tgccagaccg attataatat
    22381 taaaactgaa cagtccgata ttttgaatca ttgtcattag accataggca gtgcccagtc
    22441 ggttattgtc aactatgatg gcgaccattg gccacattac tgctggtacc agcgaaaagg
    22501 caattcccat catcgccatg ggaattatca aataagctgg aatacttgaa tttataccga
    22561 agaaactaaa ttttatattt attatatcat gaaaacccag aggagtcgcc agatcaatgt
    22621 ggtaagccat tatcagatac accggaatga tcaacagtga gcccaacgcc atcaaccagg
    22681 cttttcgacg agtgcgatct gccaccaatc caaataacgg cgtaaaaatc atcgcggcta
    22741 aagtcaacat actggataaa tttccgccaa cttcgcgaga cgtaccatgc gcctcctgaa
    22801 aatatttaat agcaaaagtc tgaaaaggaa acatcgcaga gtaaaatgtt acgcacaagg
    22861 cagttatgta ccagaaaccg gctccaaatc gaaaaacctc gcgtaattca atcttgtcct
    22921 gcttactttc ttgaggcaag agcaaccggc gagatgccag taaatccagc aaacaataaa
    22981 taccgataaa aaacaatgcc atcatcccag ctacagccgt taccatgagc ggtttttgcc
    23041 agctggtata aagactacga ccccaagtcg gcgaattgag cgccagaaat gaacccaacc
    23101 gggcaaaggt tagattcaat ccgaaggcaa aggataactc cttgcctttg aaccagcggg
    23161 ctaaaatcgt tgtaatagcc actatcattg actccgcacc taacccgaaa atcagtcgtc
    23221 cggtcgccat taataataga ttgccgctca gggcagtaat aactgcgccc aacataacca
    23281 aaatcgtgaa aattaggacc gatttacgag ttccgatccg gtcaataatc agtccgccga
    23341 ttaaaaccat gataatatta ggaaagctat aaatagcatt taatagcccg atattggcat
    23401 cactgtagcc cagctgagcc ttcagaaggt cagccaacgg gctaatactg tcatagatat
    23461 aataatttcc aaacatcgcc atactgataa aaaagaaaac taaccagcga tatcgggcgc
    23521 taggcggctt cagttcgaca tccgttccta ttttgtccat ttccagtcct tacaaaaatt
    23581 cacacaccat cataacaatt tagcggcagt taatctaagc ataccctatt tcaatacaaa
    23641 gaaatctgac gggatattgt ctggattgct ggctttattt aaatttcggg cgatttttga
    23701 ttcacgaaat aattacgagg agttatagta atggcttatg taattagcga agactgcacc
    23761 gcttgcggga cttgcattga cgaatgcccg gctgaagcaa ttagcgaggg tgatatctat
    23821 aaaatcgatc ccgatctatg cactgactgt ggggcttgtg ccgaggtttg cccctccgaa
    23881 gctatacatc cggcataatt aaatttgccc tcaaggcttt agccggatta aatatttttg
    23941 aattagagtg aattcacgtt tttgcttatt aattttgggc agaattcggg cttagggttt
    24001 ttgccgacaa ataactgatt cagaccactt acaaagtttt tccgattggc tttaaaccac
    24061 agaatgctat ttaaaataag actgattata ataagcctta taaatccttt atcaattcaa
    24121 taaatgcaat cagcgcctat ttcacttttt gacaaaattc ccgagataag ccagtccctt
    24181 gcaaatggac gacctcttgg tttagtattt aatctcgaat gccttatgag ttcacgccgt
    24241 tggcgggcgg gcgcgggtct caccgagatc cagcgtcaca ttcattgggt tgccgactta
    24301 ccggatataa taaccgtcat tctaagcgat ggttcgctta atgagctcga aactctattc
    24361 gacaaccaca agataatttt agcacccaat aacggcactg aaatttacgc ggctgatttt
    24421 tcctggaatt tacctaatct taacttgatc cggcaggaat tgaccgatat ttattgtgcg
    24481 gttcgtgaat cccttggcgc taagcttcag ccggaatatt taggtttttc tcaaagcgaa
    24541 ctctggctca aggtttcgcc gggggcggag gcagtccgaa aatcattgat tgaacagttc
    24601 aaactgcaga acccggcgca tctccgtctc ataacccaaa gtaatcaaac gactatcgtt
    24661 cccctcataa aatgggatcg cggtcaagtc attcaaaagc tgatggattt gatcccccac
    24721 ccccttaatc aaactccttt agtaatttat ttcggagtag aggagaatga cgaagcggct
    24781 tttcaatatg taaatcaagt cggcttttca gtgatcattc ataaccgcat tccacgtgct
    24841 acggaagctt gctattatct gcgtaatgtc gctgagctaa gtaaattttt gttctgggtt
    24901 cactcccgct aaggttttcc cagaaaataa aaatcctcca cgcaggagga ttaatgagca
    24961 atttttgatt tgataatcag gctaaaacgc tgactatttt cttatcccgt cctttacgtg
    25021 agaatttcac tttgccgtct attagagcaa aaagggtgtc atcactgccg attcctacat
    25081 tctcgcccgg atgaattttc gtaccatgtt gacggataat aattgtacca gccgatactt
    25141 gttcgccacc aaaacgcttg acacccagcc tttgaccgcg actgtcgcgt ccattgcgtg
    25201 aacttccgac gcctttttta tgagccatac ttactactcc tttttctctt gttcggcagg
    25261 ttcagccgat tcagttatag ttttagtttt cgcacgactt tttttaaccg gcgcggcggc
    25321 ttccggtttg gcaaattcgc ttacttgcag aacggtatat ggttgacgat gccccttttt
    25381 tacgcggtat cccttgcgtc tttttttctt aaaaattaca accttactgt tacgcttatg
    25441 ctcaagaaca gtggtttcaa tagcaaaatc ctccagatag ggtttgccaa ctttaatatc
    25501 attgccggta tgtataaata agatcttatt cgccagaaat ttagcgccaa cttcaagatc
    25561 catcttggga actgcgacgt ttttccctgg ttctaaaatc acctgatcac cggcgatttc
    25621 agcaattgca taattttggt tattcataat tctctcgtga ttactatcaa aaaaggctgg
    25681 gaaatttaga tataaagact gaataatgca agatgccggt cggatatttt tgtctgatag
    25741 ggaagactaa gacaggaatt gaggctaagg gctttgtctt agtcattatt cttacttcga
    25801 ctttagcctt atatttcttt actaatgaca aatgactttt caacgttcat ccattgtgac
    25861 aacggagatt gccagaatac ggtcgcctgc ttcaaggcgc ttgaccacat ccattccaca
    25921 aatgacctct ccaaatcgcg tgtattggta attcaattgc ggctgatcag tcagagtaat
    25981 aaaaaactgt gaaccttcgg tgtcatattc tgtccgcgcc attcccacag ctccgcaatt
    26041 aaattcaatc ggcgatcgtt cagttaaaag cgtataaccc ggaccacccc aaccgtcgcc
    26101 acgtggatca ccgccctgta caacgaaatt gggcacaacc cggtgaaaat tcaatccatt
    26161 gtaaaaacct ctctcaatgg cggatagaaa agcatcagta gtaaacggcg cataatatcc
    26221 atctaagcgg atgatgatat tgccgcattc ggtttcgagc aagatcgtcg gacgcagtcc
    26281 atattttttc agcatttcta ggtttgaatg tcgcccggca cctcggtaat cactgattat
    26341 ttctggcaca ttggcttcat aagtttctcg taaaatttga tatgccaaat ctctaatgcg
    26401 aaaatcgcga gcgttcaata acggctggat gaatggcaca gcatctggcg gctgtcgaac
    26461 ggctatcgtt tttaatattt cgataacagc gtcagcgtat tcagcatttt tgaatttatt
    26521 cagggaaggg taaagcaagg gttctgaagc tacgcatttt gcggtagaat cacgcatagc
    26581 cacggcggcg atagcgattt tcaccggatc gtcacttttt aaaccagccg caatataatt
    26641 acagaattgt tcgcagggga tgctatcctg tcggcaaaat tgctcagcca aattgatcaa
    26701 accgagatag gcaggggtag atattgctcg aaattgatgc cgggagaggc tatcaagaac
    26761 cgcaagccca gatatttggc ctgaggcggc aatgccttcg gcaaatgccg gtagcaaatt
    26821 ggtctcaact ggccagaggc caaaattcgc cagtgtctct tgaccaccgc attgactcag
    26881 aaattttacc aaggcactcc gtactgccct tgacgggtga tgcgctagaa tccgaatgtc
    26941 gtcttgaaga actgggatca attccagtga atcgggcaag ttctcaatag ccgttgctac
    27001 tacctgtgga aaggaatcat cgaaaacttg atgtaaaatt agcggatcgt gagcgacaat
    27061 gcgaatgttc cgtgcggctt caaggcgaac ttctggaaca ctatcggtca ataattgcgt
    27121 ccagatttca gccttgccgg agaaattcac aggagccaga caagtgacta tttttacgcg
    27181 ggtggcttgg cccgcagttt taagggcggt tttgaggatg tcaacgtatt cttgcatcac
    27241 agttggattt tgtacccggc tcagagttga agccgcctga taagattcgt aatgctgatt
    27301 actttgtaaa cgggaaacgg cataattcaa gctggctgaa ttataaattt ggcgttgaaa
    27361 aaaataaatc aaagcctgtc cgattaattc tggatgtgtt tcgagcaaaa aattcaaagt
    27421 cgagaggtcg ccaatttggg caagtgccaa aacagtttgc tgacgcactt cggcgtcggt
    27481 ttcgctgtac aaatgctgaa cgagcaaact ttccgccgtc tggctggcaa gttgaccaag
    27541 ggcaaaagcc gctccagtcc gcactgttgg cgactgatca tctaacaaag tggtgagctg
    27601 agtaaccagc aatgtatccc gacaattagc aatagccaaa caaagccgat aacgagtttc
    27661 agctgagggc gaattcaagt attgctcagc taccttttcc agagtgcgtc ggttttggtg
    27721 aacgagaatc tcccgaatct ccggcgtaaa aggatgcttc gaacaagtcc ggtaaaatat
    27781 taataaaaaa attacggata ttattctgga tccaagtcgg tagttgatca ttttcaccag
    27841 aaattaatga tataggctcc ggcaccaaga ccacagcgag gtacggcgcc gagatagtcg
    27901 gcactgcgaa tatgaaaata ttgcggaagc aatccgaagt tattcaaagc ctggcgagta
    27961 acccaagctt ccaggttagc ggcttcctcg aaacgagtag ccttccgagc ggctgaaatg
    28021 atccgcaaat caccaaaaac ccattcttga ctatttgtaa ttaattgttg cctggttcgg
    28081 caatagcctc gctgggcatt tgccagagtt aggtaattct taaaaagttc cagtgtgttt
    28141 cgtgtaattt gatcctgggg attgaaaatc cagttcaatg cttcgacact cgaagcgtca
    28201 gcatacaagt tgggatcgga atcttcgaga ttacccttta aagcttttaa ttgggaatcg
    28261 gtcaactttt tttcaatatt aattcgcagg ttaatggctg tctcttcgta atattgggcg
    28321 cgattttcgt cattcatttg acgcgccaga cgggcggcat ccaacaagcc gcgataggtg
    28381 caggctgagg tataagtata atgcttgtaa ggcagagatt tttcccaggg acccggctcg
    28441 gcgcgcacca gaccgctaac gtcaattaaa gtcggtaaaa catcagccat tttgcgtgag
    28501 attttcggcc agtaatattt tagaaatttg aggtcttcag taacctcaac ataacgatat
    28561 atattccaga tcatcagtcc gaaattatca agatgaatag tcagtccatt ttcatcgcta
    28621 acgctctcct ccgtgccatt gcctagatag cgataaactg agacggcata atctactcca
    28681 atcccctggt tttggtcttt cttctggtaa attttataac gtccacaacg actgttcatg
    28741 ataaactgga gggctaagcg ggcttcctca ctatgtccgg atagcaaaag ggcttcgact
    28801 gcgaaggctt gatctcttgg acgacagtaa ttctgagtag agggcggcag gctggcaatg
    28861 atttgaccgc ctgccgggaa gggctcacgg cattgagcca ttttcaatac cgccagcgat
    28921 tgcaaataaa ccttccgagc atcctcttga atattttcgg gcaataaagt ctggacttgc
    28981 cacttttgcc accagtcaat ttcttcgatc aaagcctgcc agccgggatg ttcgttctta
    29041 tatttcctca agaaaagttg gtcggcgtcg tcggcagaag gattaggatt atagaaaata
    29101 taaacggtca gccatttgct gttactctcc tgataagccc acttgccaat gtctagatcg
    29161 agattttcag aagcgtagat aaaattaaga ctgagatcat caatcttttc accttctaaa
    29221 agagcataga acacccaaaa gggcttattt aaattgaaag gacaataacc aaaaatagtc
    29281 agcttaaaac catcaccacc cagaatatgc cgaacgattc cagtaccctc caggtagccg
    29341 acctcgagcg tcactaaatt agttagcggt atttcctcct gattccgaaa gaggacgact
    29401 ttcgcctggc tgataaggtt cggcgtctca cttttttcat cgaatttgcg ataaatatga
    29461 ggctgaaaat tggtgaatgt ccgcgttttc aaattataac aacaagatgc tatcccgttg
    29521 gtgaaaggaa taatattaat cgaattttga agtacctttc ctgacggtaa aggagttttg
    29581 ggactttcga ccgtcacgat tttcagcgga ccgctataaa ttccaccctt ttcaccttca
    29641 tcgtaaacac gaaccgccag caagttatca ggctttaata aatgggcagg aaatacatat
    29701 tggcggacgt tatttttagt ggaaagcgat tccggtggga aggctccgct acgtccgatg
    29761 cagacgccat tgagaaaggt ttcgtcggca gcatcaattt ttcccagata aagaatcagc
    29821 gagtcctgcc gcaataaagc cgaatcagcc tgaaagtgat aacgatacca agccaagcca
    29881 tcataatgtt caaagccttg gttttcccag taatccggaa cgcgaatagg caaccagttc
    29941 gaatcgttcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ccttggtttt
    30061 cccagtaatc cggaacgcga ataggcaacc agttcgaatc gttcgaactg ggagctgacc
    30121 aggtcgagtc gtcgcccagc tgaaaaagcc aattaccttg taatgataat tcggcggctt
    30181 caatcggtat caacattgga cttagccaaa ggttcaataa cagaatcaca gacaaaagtt
    30241 ttttattatt gaaaaaatac cagaacgtat acttagaaaa ggctgtctta aaataacggc
    30301 tcatttaaat tatccagttt gcatttttgg gtagataccc tcaagttcaa gcaatttttg
    30361 cttcatctct aatccgccgc caaatccaac taatgcacca ttgataccga ggactcgatg
    30421 acagggaata actaatccta gcggattgga atgcaaggcg ccgccaacac tccgggcggc
    30481 gagtgggcga ccgatccatt cggctatccg gccgtaactg gtagttttgc cgaacggcac
    30541 caagccggtc caagtcaaaa cctggcgagc aaaagctgtg gaaacgagac agaaattaag
    30601 agccgtaact ttataaggct gaccggtttc caaaaaatca tagatcattt cagccgtatt
    30661 ggtcaggaaa atcggcgctg gtgaacgcgc aagttccaag ccttgctttc tcagtaattc
    30721 aatttgtcga cgctgcgttt tttccgaaaa tgttaaagct agcaaaactt ccttcgagcc
    30781 gattatccaa aattgccagc gggctgaaac tggaatcgcc cagagattag tatgattggg
    30841 aagattatat agccaaggtg gggcgcaatc aattgctgtt cgtattttag cctggcagtc
    30901 cggacaggtc cgccaatgtt caaacgctgt tgattcttcc ttgtaatcaa ggctacgatc
    30961 aagcaattga ataattttgt ccttcgcgag atgttgttta gtcatttttc tttaaataaa
    31021 tcaacatagg ttaatttaat aggactattt aattcaacaa gtcaaattgt ccactcgacc
    31081 gaaaaatatt gatagttatt attagtggta atattaaatt tgccttgtgg tagaacgttc
    31141 aataagtaaa gaccgcgccc aagaggcttt tttatgacac cgctaatcga gtacgataaa
    31201 aaaattaccc aattgatagc cgctggacgg tatgatgaag ctctctgggt tgcggaagaa
    31261 gaattgtgtt atgccgagag cagttacggt catgaacatc ttgaaacggc taaagttctt
    31321 aataacctgg gatggatttg tgatgttctc aataaagaac aatcggccga agactattat
    31381 cttaaggctc tggaagtcaa aatcgccgtc tgcggcaata cttcgcacga attgattcct
    31441 actctggaaa acatcattaa ttttttaatt aatcataatc aattagctcg ggcgtatgaa
    31501 ttcctcgtaa aattgataga cttggctgga ttgcaaaaca agctctggcg tttacgcaaa
    31561 ggtgcctatc tttgccagct ggcagaattg gaggaacaac tcggcttctc tgacaaggct
    31621 gaaaatttac tctgggaaag tctttcgttt ttgcaggcaa actatggctt cgaccatccc
    31681 aacgccggtc gcgtttttgc taaacttggc gcttttttcg aacgacaggg caatttagcg
    31741 cgcgctgaat attgttacaa ccgctcctta aaaatactga agaaacattt gtcttccaat
    31801 catccagatg tcaagtttag ccgtgatggt ttggaaaata tttactcgaa attaggcctt
    31861 aaagtcaaaa ttcgctgatt cacacttttt ccaaatgctc attttgtcca tggtactact
    31921 tttttattat tatgagaatt gccatttatc agttcgcgcc aatctggggc gagattaata
    31981 ataatataga aaatatcagc cgtgctattg ccagccaagc ggcagatctc tggattttac
    32041 cggaactatg taccaccggt tatgtcttta gtaatgtgga agaattaaag ccttatgcgg
    32101 aaaatgtcaa agaaggcgcc acggttaaag cgctctgcgc attaagccac caatatcaga
    32161 ctgcgctggt tttcggaatg gctgaaagat gcggcgatca aatcttcaac agcgccgtgg
    32221 tgctttctaa gggtgaaatt tgcgcggttt atcggaaagt gcatcttttt tatgaagaaa
    32281 aaaaattgtt ttctgccgga acagaaactc ctccggttat tgaaattcaa ggcgttaaag
    32341 ttggtgtgat gatttgtttt gactggtttt tccccgaaat ggcgcgcact ctggctctgt
    32401 cgggcgctca aatcattgct catccagcca atctggtgtt gccctattgc caggcggcga
    32461 tgctaactcg tagtctggaa aatcgcgttt ttaccgcgac tgctaaccgg atcggcagtg
    32521 aaaggtgtgg cacaaaaagg ctaactttta ccggaaaaag ccagataacc agtccgcgtg
    32581 gggaactgtt gggacaacta aaaactggtc gccccggcat tttagcgcgc gacattgatc
    32641 cgactgaggc attggacaaa tcattgacgc cgctcaatga tattttcgag gatcggcgcc
    32701 ccgagattta tcaactgggg aaatcgtcaa gatgatgaaa tctttaccaa atccggaaga
    32761 gttgcgtttg gaaaatcaac gaatgctgtg gttgagagtt tataccgacc gagtaactca
    32821 ccgcttgcaa actatgtcgt tagacgaacc ggaagcagtc agcttgctcg agcaggtcaa
    32881 gcaggaagtc ctccagcgtt ttcccgataa agagagagcc tattgcttaa tttacgagcg
    32941 tcgtttcaaa agaattctgc aaaggcgcgg attgttttta ccactaacga ttgataatgg
    33001 ggaaatgaac taaagtatgt ttaggagaat aaaatgaact accagagtgc ctatgaattg
    33061 ttctgtcgct ataccaaaac cgagagtttg cgcaaacatg ccctagccgt ctcagccgct
    33121 atgcgccatt acgccagaat atttaacgaa gatgaagaat actgggcaat taccggctta
    33181 ttacacgatt tcgattatga gatgtatcct gatgaacaaa atcaccctta tcgcggtaat
    33241 gaaattttag ctagtgaagg ttacccggaa gaaatgcgaa cggcaatcat gggacatgct
    33301 tcttacaccg gtgtcaaacg tgaatcgctt ttggcaaaaa ccttattcgc agtggatgaa
    33361 ttgagcgggt tcattatcgc cgtggctctg gtgcgtcctt ctaagagcat attcgaggta
    33421 gagccgcgtt cggtgcagaa aaaattcaaa gacaaagctt tcgccgccaa agtcaatcgc
    33481 gaggaaatta aacagggcat cactgaactc ggcgtggacg aaaccgaaca cattcaaaat
    33541 gtcattgacg ctttgaaaag tgtcgccgct gaattggggt tagctggaag ttgagatatg
    33601 aaattcttag ctgactcgat agcactaaaa tttatcggta gttgaagttc tattgtttat
    33661 ctgccattca gaattgttat tattgtattg aaaattgaaa accattacta attcgaacga
    33721 cctgatgaga attattctaa cgcaggtcga aaaacccggc cgctatgtcg gcaatgaact
    33781 gaatgctatt cgcaaagatc acacccgcac tgcggcgaca attgcgctgg catttcccga
    33841 tctctatgac gttggaatgt cgtattacgg cttccagatc ctatatcata ttttaaatag
    33901 ggaaaccgat atagccgcgg aacgagttta tgtgccctgg ccggatatga gtcggcagat
    33961 gcgatctcgg cagatcaaac ttttcagcct cgagactcac accagtctcg atcaatttga
    34021 tgtaatcgga ttcactttac ctcatgaatt gacttatgcc aatattctga caatgctcga
    34081 tctagctcaa attcccccga aagctattaa cagaaagccg tccgatccat tcatcatcgg
    34141 cggcggtagt ggagcctaca atccggaacc gctggcgccc ttttttgacc tatttgtaat
    34201 tggtgatgcg gaggaaattg tagtgccgtt ggtgcgtttg ctggcaaaag gaaggcgcca
    34261 aagtacaccc cgtcaggtga tattggaaga agctctccac cgttttgcag gaatctacgt
    34321 tccgacgttt tacaaagttg agaatggacg aactattccg gcgacatcct ttgcaccagg
    34381 agttatccag gcgaataaaa ttcggacttt gcagaatggt tattacccac aagctccatt
    34441 gatgccattg attgaaattg ctcaggatcg gttggtgatt gaattgatgc ggggctgcac
    34501 tgagggttgt aggttttgcc aggctggaat gatttatcgt ccggtgcgcg agcgccaacc
    34561 ggcggacatt atccggcaaa ttgacc
//
LOCUS       DAOSMP010000025        34214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_1295_length_34214_cov_20.267894, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000025 DAOSMP010000000
VERSION     DAOSMP010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 34214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34214
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1295_length_34214_cov_20.267894"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..1565
                     /locus_tag="PKX09_05735"
     CDS             360..1565
                     /locus_tag="PKX09_05735"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOU17358.1"
                     /translation="MRVLTLNSGSSSVKYQFIDTSTRTVLCKGLVDRIGISGTSLTHK
                     RQDGHQVVITDNIVDHQTAINSIMDILIDPTHGVAQSLKEIEAVGHRLVHAGASFKQS
                     VLINEAVREKLYECIEFAPLHNPHNIKGIEASLKKLPHTPNVGVFDTTFHINMPDYAY
                     LYAIPYSYYEKYGMRRYGFHGTSHYYVANRAAEIVGKPIEQLNIITCHLGNGCSIAAV
                     KGGVSVDTSMGFTPLEGLVMGTRCGDIDPGLILTIIDRENFSIDEANTFFNKQCGMKG
                     LSELSSDMREIENAAAAGHQRARQALDIFCYRVKKYISAYIGVLNGADLIVFTGGIGE
                     NSVLVREKSLSNLENLGILVDPARNKIAQGVEGIISSAESKINVLAIPTNEELVIAIE
                     TEKVIITSQ"
     gene            1701..1928
                     /locus_tag="PKX09_05740"
     CDS             1701..1928
                     /locus_tag="PKX09_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17359.1"
                     /translation="MAEKVMKCGVKKEKGYLYYLDKNGDIARSKMARGGNKGGGAQVI
                     MKCGIKRQEGYLYFIDKDGDVSRAKMARGRK"
     gene            2034..5396
                     /locus_tag="PKX09_05745"
     CDS             2034..5396
                     /locus_tag="PKX09_05745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOU17360.1"
                     /translation="MNDNKLMHKDWNLLAICGALLLISIIYLSHNYRKAFPAYDLKFE
                     ITRADSRDLAEKFLSRQHIDLNDYRYAVVFDYDELPKTFIEKEVGFEEATRLLCQEFP
                     IWHWSNRWFKPLSPEEIKVNLSLDGRVTYFEHFLPEEAAAERISVEAARRICQDFLVT
                     TMQMNPDEWEFIEDKTDIKPNRVDHFFTYQKKGVEISSATYRLEIGVMGNQVGKYREY
                     WKLPEQWLSNYKHLRSLNSTTALAADVLFVLILIAALVVFINQLNRKNVHLKTALGFG
                     LIIFILQFAGNINELPITIYAFDTNQSLGNYYGNYFIQTFLIALLYALLVAVITGAGG
                     VLYRRAYPQHLSMSKLFSPTGLRTKSFLINSTLGMILAVVFIAFQTAFYLIANRFGAW
                     APVEVNYSNAINTYFPWIFVLLIGFIPAVTEEFSFRLFAIPFFAKIFRSKPLAILIPA
                     LIWGFAHANYPNQPFWIRGAEVSLFGIFVGVLFWRYGILTVLVWHYTVDAIYTAVFLV
                     KTGQPYLIVTAILAAGVIVLPIIYNIVCYRRNRSFADSSGLLNGEVVFIIKPEKTITN
                     EVPEISEVKEYQSYSPRKIRKGLIISALFLLILLIPGHRVGDFYRFPIPKNEIRHTAQ
                     EFLAAKEVESSNFRIAIGLDNNYDPLMGQYVIQHSSLNQLNFIMLQYLPDLITWQVRF
                     FQPNQREEYRIYVHPMKNTVAAFSHILDETDPGARLSKASAQRKAEEFITFKKYDLSN
                     FSLIESNSDVLPNRTDYRFIWESRDEHPASVADARLRLTVTLKGDEIAAFATDYKIPE
                     DWLLAQTRQTTFQSIMTGVQIALIILILYRIIKILLKRPKMVFSGWRPAIRLALYLAG
                     LAIIVSGLNYSAALLNYDTSWTLTNWNFLWILSILLKAIGVSLLTVLIFSTVGLLYPD
                     AIQTLQKAHRPRYTRDALIASILIICGLLACNHFSNYIISMIKPELLANHQITSGLED
                     SLFPFFGALQRIIIMSILSIGAIAFIGFILQNLKGQNFRKVMTLIMLTAVFLPDNYLT
                     LSGYLAVYLQFILPILWLYFCLKYFVGANPPAYLYAAVGYFMINQIFSLLRAGSPQGR
                     LAAILLILLGLILSSWLIIEKQNVNFKSTMSN"
     gene            5432..7150
                     /locus_tag="PKX09_05750"
     CDS             5432..7150
                     /locus_tag="PKX09_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOU17361.1"
                     /translation="MENTSGIDIGYQFSDKLTDDQTLATEISNASPAVQHFIADLCDK
                     IDNLSKIGAALSAEHNLPVLLQMILEEARRFTNADGGTLYLMSDDRKWLNFAIVQTGS
                     LNIKMGGTSGNPINWKPVPLYLPDDKPNHAMVSAYVALTGEIINIPDVYNVKGFDFTG
                     TRAFDKNTGYRSKSMLVVPLRNYEDEIIGVLQILNAQDRRTGEVCPFSSTDQKLIISL
                     ASQAAIAITNARLIHDLENLFESFIQVIASAIDEKSPYTGGHIERVAQLTMLIAHKVN
                     DVNYGKYADFFMNEDNLKELRIAAWMHDVGKITTPEYVVDKATKLETIYDRINTIQTR
                     FEILKRDIKIELLKKYYSSDKETAKHNDDFLSEYARSVKYLDEQFAFIRIANIGSEYM
                     APEKQERIRQLGKYEIEINGKMQPILTAAEIENLCIPRGTLNDAERQIINNHVNVTIK
                     MLEKLPYPKKLCRVPEIAGSHHEKLDGSGYPNHIKDERLSVQAKILALADIFEALTAR
                     DRPYKKGKTLSEAMKIMNFMACDRHIDPELFEIFVKEKIYLDYARKFMDPSQIDEVNL
                     DFSVTQ"
     gene            7168..8622
                     /gene="rlmD"
                     /locus_tag="PKX09_05755"
     CDS             7168..8622
                     /gene="rlmD"
                     /locus_tag="PKX09_05755"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOU17362.1"
                     /translation="MSSPFKRHQMLELTIEDLAFGGQGIARLNDFVIFFPRSGTPGQR
                     VRARLKRVKENYAEAQIESVLEKSPLEITAPCPYFNYCGGCRFQNLPYEQQIKFLERQ
                     VIELYCHLGGYRDVQIEPIIPAVEIFRYRNKMEFAFSDRRWLIENDDLSRPIDFAIGL
                     RTPNNYYKAVDIDDCLIAPPETAEIIRLVREYARYHNLAPYNHRRHTGYLRHLVLRKG
                     IFTDQIMVNLVTSADTPEVLEPLACNLTLNLKNITSVVNTVTDNVCGTTTGKVIRLLT
                     GQAFITDRIGNLTFKISPASFFQTNTRMAIKLYEIIKQFAQPEPHHVIWDLFCGTGSI
                     ALFLARDCRQVIGFEVVADAVADAIENARLNAIENASFICANLERDFFTTPNSELNLP
                     TPDIIIVDPPRTGLTPQLIAKMRQILPNRIIYVSCNPATQVRDLKKLTADNMYQIIKV
                     QPIDLFPHTPHIETVTLLERYDFCGYTVATESNP"
     gene            9338..10330
                     /locus_tag="PKX09_05760"
     CDS             9338..10330
                     /locus_tag="PKX09_05760"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU17363.1"
                     /translation="MKKILILFSKNCWFNFPKSIKDFFYYYKDFNDSNKMLDFPASFR
                     FEITNKCNADCIFCARQYDNSKKSIMDLDLFTNGLKQYFDLGGKEISLTPTIGEPFLD
                     PLLFERIRIIKNLNSEAKISFNTNGILLGNDDNISKLFELGVDGIAISTAGFDSEMFT
                     RIYRTNGYLKIMNSLKILLIENKKRGNPINISIELRPDVNIFQVFKSADFKNYITPYL
                     SLKKINWTYHYDNWGGSVKQSNLSGNMRLAKEPKRKLKTCRMLNHLVLKLNGDIQVCG
                     CQINPEKPDELSVGNIKQQSLKEIWTGAEINKIRMSFKTGHPPSVCEKCLGYRP"
     gene            complement(10889..11128)
                     /locus_tag="PKX09_05765"
     CDS             complement(10889..11128)
                     /locus_tag="PKX09_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17364.1"
                     /translation="MKLLHEQEYANKLRSILRDYGNSGLYPEPKNLTLNPLQLRQELN
                     LNNSGLYPEPKNLTLNPLQLRQELNLNNSGLYPVP"
     gene            complement(11346..11422)
                     /locus_tag="PKX09_05770"
     tRNA            complement(11346..11422)
                     /locus_tag="PKX09_05770"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11386..11388),aa:Val,seq:tac)
     gene            complement(11472..13844)
                     /gene="lon"
                     /locus_tag="PKX09_05775"
     CDS             complement(11472..13844)
                     /gene="lon"
                     /locus_tag="PKX09_05775"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOU17365.1"
                     /translation="MAETIKNKDEKNETGHGVPEILPIMALRNTVLFPQQVIPIYIGR
                     ERSLRLIEDLPETKKRIVVVAQKEGTLENPEVDDLYEWGTIAQVLKIFNMPDGSKSAI
                     VQGLERAKIQSFIQVEPYFKGAILKAQEIYQPGIEIDAMLANLRQLYQKLIKIAPYLS
                     EEQASVFSSVQHPGRLIDRIIHLLNVPVSEKQEILEELDVRKRLEKATVILNREIQRI
                     QLGEQIQSEVQDEISKTQREYFLREQLKAIRKELGEDEGTLELKELEEKINLSKMSDE
                     ARKVAEKELDRLRKIPPASPEYTVSRTYLDWLVDLPWGIYTTDNVDIKSAQKILDEDH
                     YGLEKIKDRIIEYLAVRKLKQEQDPNASIKGPILCFVGPPGVGKTSMGKSIARAMGRK
                     FVRISLGGVRDEAEIRGHRRTYIGALPGRILQSIRKAESSNPIFMLDEIDKIGADFRG
                     DPSSALLEVLDPEQNSTFSDHYLEVTYDLSKTMFIATANMVDTILPALRDRMEILEFS
                     GYIEEEKLNIAKTFLIPKQIKEHGLTNVTVEFTDKAIRFIINSYTREAGVRNLEREIA
                     NVCRKIAREVAVNKRKKNFLIDEKAVENFLGPQRYFVDIAERLSKPGVAIGLAWTSVG
                     GDILFVEATSMRGTGKLNLTGQLGDVMKESAEAALSYIRSNATLLGLDEEAFSKIDIH
                     IHVPAGAIPKDGPSAGVTILTAVYSLLKKRKVKDNLAMTGEITLRGAVLPIGGVKEKV
                     LAAHRAGLRRVILPAQNRKDLIEIPKQIKNEMRFDFVKEMSEVLNLAIRK"
     gene            complement(13878..14822)
                     /locus_tag="PKX09_05780"
     CDS             complement(13878..14822)
                     /locus_tag="PKX09_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOU17366.1"
                     /translation="MKKHPTKLVKIDPIPILMEQAPLPIRYFTAERFLSHDNELVERL
                     KRELQFFKPRERLLATQQEDGLWKLNKNYKIEEQQKSMQFLLQLQNMMQLLDYGCTKD
                     MPAIQRGLIAILKYQKPDGKFPLLQHHQGLTLWLLVRYGLAGNPFVEKGFRWLTKRQR
                     EDGGWLSPTMLATGESAKTARSGIWTTLVITQAFSAHSRLRNTETCQRAANFLLNVYL
                     QQNHTTLFPEPDAWNHLYIDYSENGLFRGGTLRFLETLTPLPNCHSHPNFKKALDWLI
                     GQQLPSGLWPAIAGRSKEGDYAVTFRVMVVLSEIDRIG"
     gene            complement(15243..17363)
                     /locus_tag="PKX09_05785"
     CDS             complement(15243..17363)
                     /locus_tag="PKX09_05785"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HOU17367.1"
                     /translation="MKTVNFIFGIHNHQPVGNFDFVMESAYRQSYLPFIEIVEEFSAI
                     HITFHFSGCLLEWIEAHHPDYLDRIAALVKRGNVEIISGGFFEPVLAMIPDADKVGQI
                     KMMNDYIQQRFNYTAKGLWLTERVWEPHLAKPIAEAGIKYITVDDYHFLSNGKQESEL
                     TGHFLTEEQGKTLGIFPISQRLRYTIPFQDPQATIDHLAKFATDDGENVIVMADDGEK
                     FGVWPGTHEQVFTKGWLRNFFAALLKNSEWLKTLTFAEWYENHPPKGRIYLPTASYFE
                     MSEWALPWEAGEKFADLVHEFESKKRIEEVRPFIKGGIWRNFLYKYDESNWMQKKMLH
                     LSERFADIDPTSLTKPQQVELGQARLHLWRSTCNCAYWHGIFGGLYLPHLRHAIYTEL
                     LICERLLDKITGAKGFRAEVFDFDTDGENEIIIKYGNLHTAIAPHRGGMITELSLVNK
                     AFNLLNNIQRYHESYHRKVALADKVENSNASGSIHDLVLSKEKGLDKYLKYDKYPRKN
                     LLDHVIHPSVSLERFRDGEYYEDSDFITGRYSPELNEKTRTLTLSKDGWVNWQRFRIK
                     KAITFGKEALKVTYILTNIGERENVFRFGPEFNFALLGGNSPDRYYILDGEKPAEAAL
                     NSSGVVTGVKALGVVNEWDKFQAEVIAPQAVEFWRFPIETVSLSEAGFERIYQSSVII
                     PWFDVRLNPEETATFSIELTTTEL"
     gene            complement(17385..18395)
                     /gene="gap"
                     /locus_tag="PKX09_05790"
     CDS             complement(17385..18395)
                     /gene="gap"
                     /locus_tag="PKX09_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOU17368.1"
                     /translation="MAIKVAINGFGRIGRLVLKASYKNPQVDIVAINDLTDAKTLAHL
                     LKYDSVHGKFDGTVSVDGNYLVINGKKIEVFAQKDPSLLPWGKLGVEIVVEATGKFRN
                     REGMQKHITAGAKKVILTVPADSKEDVDVTIVPGVNDEMLTKNSIFVSNASCTTNCLA
                     PVAKVLNDNFGIKRGLMNTIHSYTNDQVILDFPHKDLRRARAAAVSIIPTKTGAAKAI
                     GLVIPALDKKMDGFAMRVPTPDGSVVDLTCELEKPATKEEINAAMKAAAEGPMKGILE
                     YTEDPIVSIDIVGNPHSSIFDAGMTKVLDGNFAKIISWYDNEWGYSCRVAELIVKMGL
                     LK"
     gene            complement(18734..19528)
                     /locus_tag="PKX09_05795"
     CDS             complement(18734..19528)
                     /locus_tag="PKX09_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOU17369.1"
                     /translation="MSQISNAKSLFNPAYDILALDFDGVIVDSIAECLVVGHNAFQSA
                     IGRSEKINDLSQLPREIQQESRRLRNFIRHGEDYVYIFKAIADDFSIHNQADFDDYIE
                     KNIDVKEKFRTQFYLERARFLREEPLRWLALNPFYEGMADFLKNFSPPEHLYIITTKK
                     LEYVQVILDTAGTPLQRKNLFAANSQKGKPEIIADLLQKIGLSPGNFCFVDDQVDTLI
                     RLKPLKIRLFIAGWGYTNQEQIRQAETEGIPTLTLNEFYQLFSRSD"
     gene            complement(19569..23363)
                     /locus_tag="PKX09_05800"
     CDS             complement(19569..23363)
                     /locus_tag="PKX09_05800"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOU17370.1"
                     /translation="MCFQVNGRYWNSVRINFQQSLLKITRLGVIISLLIMLIGLVFFD
                     SADAAEKYEPLRLIRADKLEQFYRFDVPIKRLTGDVLFQKGGLQLACDLAYWLEKDQR
                     ADFYRNVVATSDNKVLKADSLVYFSERDSIVARGRPILFDSTLSITARRLIYLVKDEI
                     AHAQGNVLLKDSSRSVRANLIHYYSNEKKSVAIGQAVLYDVKRHLELASDSLIYFNKT
                     GNIFALNRPMLTRFDTAGVESFRIYADTIRLVEKEGNFQAIGNVRIQSKDFTAYSKTL
                     TYIDSLEVATLTDNPRVLSDGQELTGKLMRLYFEDEKISALFIDEDARANAVGKAYLP
                     ADSDSLAHGDSIRTYDEITGKFMEIYFRDDQADSIVVAVMATSLYNMLEDSIILGINE
                     ISGDTIRLKFIDQKINTITVVGGTQGRFIPHETNTDLDTTVIYSAEQIDYFVDRKLTD
                     LYLNAKIQYADIELTAGKININWNENLLYAYPLSPPPYDSTNADLPVLNQRGREPFVG
                     ESMVYNLKTQRGRIVSGKTKEQDGYYYGDKISKVDKKVFYVNDCVYTTCDIPDKPHYY
                     FRSKQLKIIFKDKVIARPVIFYIHDIPLLGLPFIIIPDQSGKRHSGWIMPSYGESRIG
                     GGFIRGLGYFWAPNDYYDFRLTGDFYDKQGVILDYRLRYAWRYKLNGTISGKYTNDFF
                     SNYPEKQWAFNITHNQTINPTTRLNVNGKFVSNESLYKKYSYDLEDRLDQQLISNATL
                     SKSWTGKPYSLSVNFNQTTNLQANALSQIEPTDSNLTIAHISRSLPNISFTRSSKPLI
                     PLKPHQDVSRSKWYNNIYFSASSYLKNNQNIFYQSIYNPATDSLEWQEQNIAKNAVTY
                     NFSFTSSQKIFGFLTTNQTVNLDEGWVFKYDMPLYDEDHRFQLENGKIKTETVEGFKA
                     RHTGSATISAQTKAYGLFPVRIAGLQAVRHVVTPSVSMTFRPDFSREIFGWDPGYIIG
                     GIDTTGTARTFDPLTNTMLGALSSYETRTMSMSLSNLFQAKLGQGEKTRKLDLFTLNA
                     STGYNFAADSLNWSVISSSFRTQVTKKIALNLNATHDLYAYQNNRRINEWNKTWNGIP
                     IPRLTSVSASTGFSLSGKRFGSPRQDMTTPASDILPDTTDINIIMPSYGETNINKLQE
                     STPKQGSELWNANFNLRYSLSQTNPLKRTESLFMTTNLTLNLTSKWKIVYQNSMNLIE
                     KKIVSQSVSVTRDLHCWQLAFSWTPSGYGKQYLLLINIKSPTLRDLKYEERGGRRSSL
                     GF"
     gene            23682..25211
                     /locus_tag="PKX09_05805"
     CDS             23682..25211
                     /locus_tag="PKX09_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17371.1"
                     /translation="MKKYSAGFGLILLVSIGFAQTYHITDFIPDLLKTWQVYGTLSSS
                     LSGDDSEAERKDIGTSEYTDEDKNSTISLTPTVYYKYIWLTTNQEFSINSNIQGLYER
                     SNNRSDQNNFNLGFSIDDVTNKYIDGRFQGIIEYTRYLFCQNRLGVSVGTNFNLNENN
                     RKTTEIEKIQSSITDHWDKNNSESHHSHHSVSLYPGIVYGRIYNGNYAAKAEEILTEL
                     KKQSLLQRDLTVAEFQEFSHLIMNHVAAYHYDSRIKNIETLTDIIDYLESIGVLKELD
                     IASFVTINDIYLFSPARKIRNFGKQFYLQSGLRYSPDVYNYEDENWYRSWYADNGIFY
                     HDSLLENYRTVTSQKNRVLSHSYWYKIGFEHYQVKSWHLWYNYGAYFDHSFSPQRYTE
                     KLKTERIDITSRSFNRSRSDEVAAFAGFNYQFTSRSVLSLPVSITYSRDRTHWNSGSK
                     NIVYFTGVTMGAEFTYYLTPKWSLTSSGQIIHSYYRYSLDKQTNRSTYGTLRFSMTYY
                     W"
     gene            25321..25695
                     /locus_tag="PKX09_05810"
     CDS             25321..25695
                     /locus_tag="PKX09_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU17372.1"
                     /translation="MKRSTLVRFAIFFSLTLVLIFFSGCATEAQRHYDLGKWYYEKGL
                     INEAILEFKAATQKDPDFYQAHQSLAIAYAKKGWYDYALKEAEISFDLHPSDESYNLI
                     QIIRHKKILEPAYESALTDTLQ"
     gene            25831..26667
                     /locus_tag="PKX09_05815"
     CDS             25831..26667
                     /locus_tag="PKX09_05815"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HOU17373.1"
                     /translation="MKHILSVSRRSDIPAFYGEWFLQKVREGSVTLPSPFSRRIQVVS
                     LRPEDVSAIVFWSKNYQPFWPILKQIKPLYNGRFLFHLTMNGFRSSAKILLEPGAPDT
                     EKALKTAHLLCDEYSPEQILWRFDPIIFSNLTPPEERLEAFSKLAEQLRGLTRRCYVS
                     FVDLYGKVKRRFNAAEASGKIQFDKPELPEQVDFLENLKPIATTTGIQLFTCCEDEIG
                     AAAGLPKGHCIDAELLKRLFPDEEFPTQMRPTRPQCGCYISRDIGAYRTCRHACLYCY
                     AQ"
     gene            26882..27739
                     /locus_tag="PKX09_05820"
     CDS             26882..27739
                     /locus_tag="PKX09_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOU17374.1"
                     /translation="MSKTTKTSSRDSSRTLSRYLEEISHFSPLSPEKEIELARRIKNG
                     DRQAAKELTEANLRFVVSVAKDYQGQGLPLMDLINEGNLGLLKAVERFDETRGFKFIS
                     YAVWWIRQSILQALSEQSRLVRLPMNRVGTLTKIAREAEVLEKQNERSPRYDEIAAGL
                     DVDREVVNDTLHIARRHRSLDEPMGDSDSNSLIDVLTDENVTDPDWQLMVESLSHDIN
                     TSLAALNKREREVLKMYFGIGSEYALTLNEIGEHFNLTRERVRQIKERAIRRLRHKSH
                     SSLLKTHLG"
     gene            27938..29293
                     /gene="glmM"
                     /locus_tag="PKX09_05825"
     CDS             27938..29293
                     /gene="glmM"
                     /locus_tag="PKX09_05825"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOU17375.1"
                     /translation="MLIESLSGVRGYDTDLTKELISSYAAALKELLACRRVVLGRDTR
                     ASGVRIREQLTEALRRYGVAVIDLGICPTPTVEMAVVQHRADAGVVITASHNPLPWNG
                     LKFLGADGLFLDPEQMLWIKQNRQSLLAKYSDAHFSPGDLIFYEWANRDHINAILKLP
                     YVEMESIRRQHFKVAVDAVNGAGSKIIPELLKEFDCEVIAIHCDPHQPFPHPPEPLPE
                     NLTDLCQAVKDNSADLGLAIDPDGDRLAIVSDKGVPISEEYTLVLATKLVLSKTGKKN
                     QKVVANLSTTRALEDLAAQYDAEVIRTPVGEINVARRMQMEKALIGGEGNGGVILPEA
                     HLGRDSLVGMALVLQLLADEKRSLSAVMPTLPQYFMVKRKIPRGKADFNEIMNHLIQS
                     VDNAEINTEDGLKFSWSDRWVHIRTSNTEPIMRIYAEAPTSQAADQLADRFVTLLQKF
                     I"
     gene            29327..30028
                     /gene="deoC"
                     /locus_tag="PKX09_05830"
     CDS             29327..30028
                     /gene="deoC"
                     /locus_tag="PKX09_05830"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOU17376.1"
                     /translation="MTYASIINPEEARLAIAHLIDHTLLKPEATPNDIVRLCEEALEY
                     HFATVCVNPSFVELCANCLYNSKVAVATVIGFPLGANTMEIKAAEVEQALNHGATEFD
                     MVINIGRLKAGDLEYVEDDIHAVVDAADGNLVKVIIETALLTDEEKKLACHLALKAGA
                     HFVKTSTGFSKYGATVEDVRLIRSIVGNQMGVKAAGGIRTYSDAILMIEAGANRIGTS
                     AGVQIVQESKQFKNH"
     gene            30241..31113
                     /locus_tag="PKX09_05835"
     CDS             30241..31113
                     /locus_tag="PKX09_05835"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOU17377.1"
                     /translation="MGIPGLTIIGESINDSVPSTHDLFQANDLDGIVGLARFQAEKGA
                     AYIDVNVGRRSPAFMAEVVQKIQQHVSVPLSIDTPDPEIALAGLEAYNPKLAGNRLPV
                     LNSISEARLEIFDLYSKQPFIPILLITEGKNESGEIVMNKTPEQNYSTAKSLVEIARE
                     RIQGVANNRLILDPGIAPIGSDMEGNFKRLMKVMEVIYQDPDLAGINMSVGLSNFTVM
                     LPSKKSDGSPVKSPLESAFLTLAMPLGLNFIVGSVKRKYEILPDDHPAVQCLKNILVS
                     DGLNAVLQIMNFIA"
     gene            31155..31598
                     /locus_tag="PKX09_05840"
     CDS             31155..31598
                     /locus_tag="PKX09_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOU17378.1"
                     /translation="MDLVTFDKPPISARERKLSRFMLFFEDLIKVPVFHCQHCGECLL
                     SSTGFVCSQRCPKRLRNGPCGGTRDDGHCEVFPERKCIWYRIYRRSRLLGRIKLLYKI
                     NRIHNWNLEHTSAWLNVFRKRIEGLVLFVPKNLDIENINDTQRKN"
     gene            31576..32466
                     /locus_tag="PKX09_05845"
     CDS             31576..32466
                     /locus_tag="PKX09_05845"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOU17379.1"
                     /translation="MTLREKINSGKFIVCGEIGPPQSCDGDVIRRKAQYFKGIADAVN
                     ITDNQTAIPRLSSIASAKILLDEGVEPIIQMTCRDRNRLAIQSDLLGAAALGIRNVLC
                     LTGDYQKLGDNPEAKGVFDLDSIQLIATVAQMNSGYLLSGREMKKAPEFLIGGAANPF
                     AVPFEMRLIRLYKKVKAGVNFIQTQPVFDPELFIRWMEKVVEMGLHEKVAILAGVMPV
                     KSVNTLLHMREDVPGVKINDEYIRRMEKAADPQAEGVRIAVETIQTVKNIKGIRGVHI
                     MPFMWESIMPTVVKEAGLCN"
     gene            32484..33149
                     /locus_tag="PKX09_05850"
     CDS             32484..33149
                     /locus_tag="PKX09_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOU17380.1"
                     /translation="MQDIIEKIALCVEKGKISRNHPFPPDMKDQDGADEIAAAALKEG
                     VPPDVLLQASMLGMERIGKKFSENLVFVPHLLVAAQAMNAVLKHLQPYLEAGSVKRKG
                     KFVIGTVAGDLHDIGKNLVAMIVRGGGFEVIDLGTDVSTQKFLSAVEQHPGCFVGLSA
                     LLTTTMLNMGKIVSAIRETFPTTKVLIGGAPVTEEFRAQIGADFYSPNPQQAVEYLNQ
                     FVS"
     gene            complement(33237..34136)
                     /locus_tag="PKX09_05855"
     CDS             complement(33237..34136)
                     /locus_tag="PKX09_05855"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HOU17381.1"
                     /translation="MKRAIYITISLALVISVFISELLGEGKPFYQMSNPEIDSLLTIV
                     STQNLTITDRINLFSDKFLGMPYNQNCAGDGPYALYEPWPLVNFKETNCMVFCEHVLA
                     LSISDSWDNFFNNLQQIRYKDGIIGMRTRNHYTMADWLPENSWLLEDVSHQVGGQYIQ
                     QMTRTILHRKFFEDKGIADLRYVKPDRTITLEYLPKEYLFKVENNICNGDILVLIFAN
                     KSDIFSAHMLISVVKNGKLLIRESSSRKMTTCETPFTEWGKSIQSQDQYAGLCFIRIR
                     AELNTPGRIILPWEITELKKNHE"
BASE COUNT         9667 a         7086 c         7394 g        10067 t
ORIGIN      
        1 tttaaacaac atctatccac ctgtcgggta gacaggtcag acaggaaggt gagccatttt
       61 tttgtctttt gccacacttt ttgagatgct tctgctattc agatatcaaa tggtttcacc
      121 cataataacc cttaaaaaaa tattcgcgct cttttaaaat attttggcta ttacaatcat
      181 actttttaat cacttgtgtc tttaatcata taccagtctt cactctctaa aaatagtttg
      241 aaaatagacg aagatttact aatatttacc ataaattgcg aaattgaagt ggcactaata
      301 aaaattttac cctcattcta ccaacaattt gcagctcaaa atagtggaaa aaatataaca
      361 tgagagtact gacgcttaat tcaggtagtt cgtctgtcaa atatcaattt attgatacca
      421 gcactcgaac cgtattatgt aaaggattag ttgaccgaat tggcataagc ggcaccagtc
      481 ttactcataa acgccaggat ggtcaccagg tcgttatcac cgataacatt gttgatcatc
      541 aaacagcgat taattccatt atggatattt taatagatcc tacccatggt gtagcccaga
      601 gtcttaaaga aattgaagca gttggtcatc gtttggtgca cgctggggct agttttaagc
      661 agtcagttct aattaatgag gctgttcgcg aaaagttgta cgaatgtatc gaatttgccc
      721 ctctgcataa cccacacaac attaaaggta tcgaggctag cttgaaaaag ctgccgcaca
      781 ctcctaatgt aggagtcttt gacactacct tccatatcaa tatgccagac tatgcatatc
      841 tgtacgccat tccttacagc tactacgaaa aatatggtat gcgacgctat ggattccatg
      901 ggacttcgca ttattacgtg gctaaccgag cggctgaaat cgttggaaaa cctattgaac
      961 aattaaatat aatcacctgc cacctgggca acggttgtag tatagcggcg gtcaaaggcg
     1021 gtgtttcggt tgatacttcg atgggtttta ctccgctgga aggtttagta atgggtacac
     1081 gttgtggtga cattgatccc ggattgattt taactattat cgatcgtgaa aatttttcta
     1141 tagacgaggc taatactttt ttcaataaac agtgtggcat gaaaggactt tctgagcttt
     1201 ccagcgatat gcgtgaaata gaaaatgcgg cggccgccgg gcatcaacgt gctcgccagg
     1261 cactggatat attttgttac cgcgtcaaaa agtatatctc cgcctatatc ggagttctta
     1321 atggtgctga tttgatcgtt ttcaccggcg gcataggtga aaattctgtc cttgtgcgag
     1381 aaaaatctct atctaacctt gaaaatctgg gtatcttggt tgatccggca cgtaataaga
     1441 ttgcccaagg cgtcgaaggt attatcagca gtgcagaatc aaaaattaat gtcctggcaa
     1501 tcccgactaa tgaggagttg gtgattgcta tagagactga aaaagtcata atcaccagtc
     1561 aatgaagcca atttttccac ttattttaca ttaaatgccg aaaaaaacgc ttttagacgg
     1621 aagattttct tgacatttta ctttttgtcc cttaacattt acctataaat gttgtaaaca
     1681 aactttagga ggaaattact atggcagaaa aagtcatgaa atgtggtgtg aagaaggaaa
     1741 aggggtatct gtattacctc gataagaacg gcgatattgc tcgttccaag atggctcgcg
     1801 gcggaaataa aggcggcgga gctcaagtta ttatgaaatg cggcattaaa aggcaggaag
     1861 gctatctcta ttttatagat aaagatggcg atgtctcacg cgctaaaatg gctcggggca
     1921 gaaagtaaac aggccgtaat tctgctgtca ttaataacta aaagactccg tttcaacgga
     1981 gtcttttgtt ttatataaaa tggaattatt ttaaggcgtg tttttagaac actatgaacg
     2041 acaataaact gatgcacaaa gactggaatt tgctggcgat atgtggggca ttgctcctga
     2101 tttcaattat ttatctcagc cacaattatc gcaaagcttt tccggcttat gacctgaagt
     2161 tcgaaataac gcgcgccgat tcgcgtgatt tagctgaaaa attccttagt cgccaacata
     2221 ttgatttgaa cgactatcgc tatgcggtgg ttttcgatta tgatgaattg ccaaagactt
     2281 ttatcgaaaa ggaagttggt tttgaagaag ctacgcgcct attatgtcag gaattcccca
     2341 tctggcactg gtcaaatcgc tggtttaagc cgctttcacc ggaagaaatc aaggtcaatc
     2401 tctcgttaga cggcagggta acttactttg agcatttttt gccggaagaa gcggcggcag
     2461 agcgcatttc agttgaagcg gcacgccgga tttgccagga ttttctggtg acaaccatgc
     2521 aaatgaaccc ggacgagtgg gagttcattg aggataaaac cgacatcaag cctaatcgcg
     2581 tcgatcattt ttttacttac caaaagaagg gagttgaaat ttccagtgcg acttaccgac
     2641 ttgagatcgg cgtgatggga aaccaggtcg gcaagtatcg tgaatactgg aagttacccg
     2701 aacaatggtt gagcaattat aaacacctgc gttcgctgaa ctcgactacg gctttagccg
     2761 ccgatgtttt attcgtatta attctgatag cggcattggt tgtttttata aatcaactta
     2821 accgaaaaaa tgttcacctc aagacagcgc tcgggtttgg cttgattatt tttatactac
     2881 aatttgctgg taacatcaac gaattgccga ttacaattta tgccttcgat accaaccagt
     2941 cactcgggaa ttattatgga aattatttca ttcagacttt tttaattgcc ctgctttatg
     3001 ccctcctggt ggcagtaatc actggagccg gtggagtgct ttatcgtcgt gcttatcccc
     3061 aacatctttc tatgagtaag ctatttagtc caaccggttt acgcaccaaa tcatttttaa
     3121 tcaattcaac tctggggatg atcctggcgg ttgttttcat tgcttttcaa acggctttct
     3181 atctcattgc caatcgtttc ggggcttggg cgccagtcga ggtgaattat tccaatgcta
     3241 ttaataccta ttttccatgg atttttgtcc tactgatcgg atttattccc gccgtaacgg
     3301 aggaattttc ctttcgtctt tttgctatac cgtttttcgc gaaaattttt cgctctaagc
     3361 cactggcgat cctaatcccg gcgctcattt ggggatttgc tcatgccaat tatcccaacc
     3421 aaccattctg gattcgagga gccgaggtta gcctttttgg catttttgtg ggtgttttat
     3481 tttggcgata tggaattttg accgttctag tttggcatta tacggttgat gccatttaca
     3541 ccgctgtatt tttagttaaa actggccagc cgtatctgat cgtaaccgct attttagcgg
     3601 caggcgtgat agtgttacct atcatttata acattgtttg ctaccggcgc aatcgtagtt
     3661 tcgctgattc gtccggcttg cttaacggtg aagtggtgtt tattatcaag ccagaaaaaa
     3721 ccattactaa tgaagtcccc gaaatttcgg aagtgaaaga gtaccagagc tactctccgc
     3781 gtaaaatccg caagggttta ataataagcg cactattttt attaattcta ctaataccag
     3841 gtcacagagt tggtgatttt tatcgctttc cgataccgaa aaatgaaatt aggcataccg
     3901 ctcaagaatt cctggccgca aaagaggttg agtcgagcaa ttttagaatt gctatagggc
     3961 tggataataa ttatgatcca ttgatgggtc agtatgtaat ccagcattct tccttgaacc
     4021 agctcaattt tataatgctt caatatttac ccgatctaat tacttggcaa gtgcgttttt
     4081 tccagcccaa ccaacgagag gaatatcgca tttacgttca cccaatgaaa aatacggtgg
     4141 cggcattcag ccatatcttg gatgaaaccg accccggagc gagattgtca aaagctagtg
     4201 cccagcgcaa agctgaggaa tttataacct tcaaaaaata cgacctgagt aatttttcac
     4261 taattgaatc gaattcggat gttttgccca accgcaccga ttatcgtttt atctgggaga
     4321 gtcgggatga acacccagcg agtgtcgctg acgcccgttt acgacttacg gtaaccctca
     4381 aaggtgacga aattgcggct ttcgccaccg attataaaat tcccgaggat tggcttttgg
     4441 ctcaaacgcg ccaaacaact tttcaatcca taatgaccgg tgtgcaaatt gctctgatta
     4501 tcctaatact ttatagaatt atcaagatcc tattaaaaag gcctaaaatg gttttcagcg
     4561 gctggcggcc tgctattcgg ttggcgctct atcttgccgg attggcaatt atcgtcagtg
     4621 ggttaaatta ctcggcggct ttgctaaatt acgacaccag ctggacgctg actaactgga
     4681 attttctgtg gattttatca attttgctga aagcgattgg agtcagctta ttgaccgtcc
     4741 tcattttttc aacggttggg cttttatatc cggacgctat tcaaacttta cagaaagcgc
     4801 atcggccgcg atacacacgc gacgctctga ttgcttccat tttgatcatt tgtggacttt
     4861 tagcctgtaa tcacttctca aactacatta tctcaatgat aaagcctgag cttctcgcca
     4921 atcatcaaat tacatctggt ctggaagatt cgctcttccc atttttcggt gctttacaga
     4981 gaataataat catgtcaatc ctgtcgatcg gggctatcgc ctttatcggg ttcatattac
     5041 aaaacctaaa aggccagaac ttccggaaag taatgactct tattatgctg actgctgttt
     5101 ttctaccaga taattacctg acactttccg gttatcttgc cgtttaccta caattcattt
     5161 tgccgatcct atggctatat ttttgcctga agtatttcgt cggtgccaat ccaccggcat
     5221 atctctacgc ggcggttggt tattttatga ttaatcaaat tttcagcctg ctaagggcag
     5281 gttcacctca aggtcgtctg gcggctattt tgttaatcct gctgggattg attttgagtt
     5341 cgtggctaat tatcgaaaaa cagaatgtaa attttaaatc aacaatgtcg aattgaactt
     5401 tatgaaaaca caatgagtat gagggtataa aatggaaaac acctctggaa ttgacatagg
     5461 ctatcaattc tccgataaat taactgatga tcagactttg gcgactgaga ttagcaatgc
     5521 ctcgccagcg gttcagcatt ttatcgccga tttgtgtgat aaaattgata atctgtccaa
     5581 aatcggtgcg gcactatccg ccgagcataa tctcccggtt ctgctccaga tgattctgga
     5641 agaggcgcgc cgttttacca atgccgacgg tggcacgctt tatctgatga gtgatgatcg
     5701 caaatggctc aatttcgcga ttgtgcagac cggctcgctt aacatcaaga tgggcggcac
     5761 ttccggcaat ccgatcaact ggaagccggt tcctctatat ctgccggatg ataaacccaa
     5821 ccacgctatg gtttcggcgt atgttgcact aaccggagaa attatcaata tcccggacgt
     5881 ttacaacgtt aagggttttg actttactgg gacacgcgcc tttgataaga ataccgggta
     5941 ccgctccaag tcaatgctgg ttgtcccttt acgcaactac gaagatgaaa tcatcggcgt
     6001 tctgcaaatc ctcaatgctc aggaccgccg cactggcgaa gtttgcccct tctcatctac
     6061 cgatcagaag ctgataattt cgctggcttc acaagccgcg atagccatta ctaatgcccg
     6121 cctaatccat gaccttgaaa atttattcga atctttcatc caagtcattg cctctgctat
     6181 tgacgaaaaa tcaccctaca ccggcggtca tatcgaacgt gttgcccaat tgaccatgct
     6241 tatagctcac aaagtcaacg atgttaatta cggaaaatat gccgatttct ttatgaatga
     6301 ggataattta aaagaattgc gtattgcggc ttggatgcac gacgtcggta aaattaccac
     6361 tcctgaatat gtggttgata aagccactaa attagagact atttacgatc ggataaatac
     6421 cattcaaact cgttttgaaa ttttaaaacg cgatattaag atcgagttgc ttaaaaaata
     6481 ttactcgtct gacaaagaaa ccgctaagca taatgatgac ttcctctcag aatatgcccg
     6541 aagcgttaaa tatctggatg agcagttcgc tttcatccgg attgcaaata tcggtagtga
     6601 atatatggct cccgaaaaac aagaacgcat tcgccaactt ggcaaatacg aaatcgagat
     6661 aaatggcaaa atgcaaccga ttttgaccgc cgctgaaatt gaaaacctct gtattccacg
     6721 tggaactttg aacgatgccg aacgccaaat tattaataat catgtcaatg ttacgatcaa
     6781 aatgctggaa aaattgccct acccgaaaaa actatgccgc gtgccggaaa tcgctggtag
     6841 tcaccatgaa aaactagacg gctccggtta tcccaatcat atcaaagatg agcggctttc
     6901 cgtgcaagcc aaaattttag cactggctga tattttcgag gcgttaaccg cccgcgaccg
     6961 accatataaa aaaggcaaaa ctctatctga agcgatgaaa ataatgaatt ttatggcttg
     7021 cgatcgccac attgatccgg aactatttga aatttttgtc aaggaaaaaa tatatctcga
     7081 ttatgcaaga aaatttatgg atccttcaca aattgatgag gtaaacctcg atttttcagt
     7141 aacgcaataa aatcaggatt ttcacttttg agttctcctt ttaaacgcca tcagatgctg
     7201 gaattgacta tcgaagacct ggctttcggc ggtcagggga ttgctcggct gaatgacttt
     7261 gtcatctttt ttccacgcag tggcacgcca ggtcaacggg tgagggctcg ccttaaacgg
     7321 gtcaaggaaa attacgccga agcccagatc gaatcggttc tggaaaaaag ccccttagaa
     7381 ataacagcgc cctgcccgta ttttaattac tgtggcggat gtcgttttca gaatttgcct
     7441 tatgaacagc agatcaaatt cctggaacgc caggtcattg aactttattg ccatctcggc
     7501 ggctatcggg acgtccagat tgagccgatt attccggctg ttgaaatttt tcgctatcgc
     7561 aataaaatgg aatttgcttt ttccgaccgg cgctggttaa ttgagaacga tgacttgagt
     7621 cgcccgattg attttgctat cggtcttcgc acgccaaaca attattacaa agccgttgat
     7681 attgacgatt gtctaattgc accgccggaa acagccgaga ttattcgact ggttcgcgag
     7741 tatgctcgct accataattt agcgccatat aatcaccgac gtcacaccgg ctacttgcga
     7801 catttagtat tgcgtaaagg cattttcact gatcaaatta tggtaaattt ggtgacttct
     7861 gccgatacgc cggaagttct cgagccgctt gcgtgtaatc taactctaaa tctgaaaaac
     7921 atcacctcgg tagttaatac tgttaccgat aatgtttgtg gtacgaccac cggtaaagtt
     7981 attcgtcttc tgaccggtca ggcattcatc accgaccgaa ttggtaactt gacttttaaa
     8041 atttctccag cttccttctt tcaaacgaac acccgaatgg caatcaaact ttatgagatt
     8101 attaagcaat ttgctcaacc ggagcctcac catgtaatct gggatttatt ttgtggcaca
     8161 ggtagcatcg ctcttttttt agcacgcgat tgccgccagg tgatcggttt cgaggttgtg
     8221 gcggacgcag ttgccgacgc aattgaaaat gcccgactca atgcgatcga gaacgcctcg
     8281 tttatttgcg caaatctcga aagagacttc tttactacac caaattcaga gctcaatttg
     8341 ccgacgccgg atattatcat tgtagatccg cctagaactg gattaacgcc ccaactcatc
     8401 gcaaaaatgc gtcaaatcct gccgaacaga ataatatacg tttcctgtaa tccagctacg
     8461 caagttcgtg atttaaaaaa gctaacggct gataatatgt atcaaattat taaagttcaa
     8521 ccaatagatt tatttccgca taccccgcat atcgaaactg taactttatt agaacgatac
     8581 gacttttgtg gttacaccgt tgcgacagaa agtaatccat aaagttttga ttcacttgaa
     8641 aaatggaaaa atgtttggat tgaaataaga gaccaaagca aggcaagcga gatgaattca
     8701 atttgttctc ggcatagcgc cacaaagaaa gtggaaattc ttcgtgaaac ccttagagaa
     8761 ttaggttcct attcgtaaaa ttattcctcg tcatgtaagg ctatatcgtt ggaacgataa
     8821 gaaatcctcc tctaaagata atggatttct ctaatccaaa gctctcccga acacccgttt
     8881 gcccgattag taggtaagct tcatgatact aaatttcaat catcagggct ttctccttgt
     8941 ttaaccaggt attttacaac tgttaaaata ccagagaaaa ccctttgtta aaaagcgttc
     9001 aaaaaactgt actgagttaa tcctatttat ctctaaaagg tagataccat gtattgatac
     9061 tataccagcg ctaaagattt tcaacttgtc aaattcatgt ttaataatta aaataaacgg
     9121 gagttagtaa aatgatagat cgcaataact tataaataat ttattctttg aagattattt
     9181 gacaaacaca tttaggcttt ttctctatac atcaaaggtg aaaatgattc ttatattttt
     9241 tggtatcagc gagtgagcat taacacaaga ttaaaattag gaaaatccct aatatcagaa
     9301 cagaaagatt aacgatttat gatatagata gctgtaaatg aaaaaaatcc tgatactatt
     9361 tagtaaaaat tgttggttta attttccgaa atctataaaa gatttttttt actattataa
     9421 agatttcaat gattcaaaca aaatgctcga ttttccagct tcgttcagat ttgaaattac
     9481 aaataaatgt aatgctgatt gtattttttg tgctcgtcaa tatgataata gtaaaaaaag
     9541 cattatggat cttgacttat ttactaatgg attgaaacaa tattttgatt tggggggtaa
     9601 agagatttca ttgactccga caattgggga gccgttttta gatccattgc tttttgaacg
     9661 aattaggatt attaaaaacc ttaattcaga ggcgaagata agttttaata ctaatggcat
     9721 attacttgga aacgatgata atatcagcaa attatttgaa ttaggagtag atgggattgc
     9781 aataagtacg gctggatttg attcagaaat gtttaccaga atctatcgaa caaatggcta
     9841 tcttaaaata atgaacagtc ttaaaatatt gttaattgaa aacaagaaga gagggaatcc
     9901 gattaatata tccatagaac tccgacctga tgtgaatatt tttcaggtct ttaaaagtgc
     9961 cgattttaag aattatatca cgccctattt gagtttgaaa aaaataaatt ggacatacca
    10021 ttatgacaat tggggcgggt ccgttaaaca aagtaactta tcgggtaata tgcgattagc
    10081 taaagaacca aaacggaaat taaaaacgtg tagaatgctc aatcacttag tactaaaatt
    10141 aaatggtgac atccaggttt gcggttgtca gataaatcct gaaaaacctg acgaattatc
    10201 tgtgggtaat atcaaacagc aatctttaaa agaaatctgg acaggcgccg agatcaataa
    10261 aattaggatg tcatttaaaa ctggacatcc gccatcggtt tgtgaaaaat gccttgggta
    10321 tcgtccataa aatgatttga taaatggtta aaatgattct ttaaataggg tttataaaag
    10381 attctggatt atacaaaggt tttaaaagga ttcaatgcat aatatccaga atcattaatc
    10441 acttaaaatt acttgtaaat gctgaattag caataaaaaa attctaatta cccaatctta
    10501 ctgccttttt tgggataatt acaattctcc gcccggttaa agtaataaaa gtactaatgg
    10561 attaggtctc agtctatgtc ttatccttct tttagccaaa atgaggacta agtttgaggg
    10621 gcaaaatttg aatatgtagg gcgatttgct aaatcgtcac attggcattg gttgaattgc
    10681 aaactcgatc tatattattt ttttactcag tcttagcctt ttttctaatg accaatgact
    10741 tatctcagtc tcttttctca accttaacct ttttacttgt atcaacgccg gtgtgtcgag
    10801 accaggcgaa gtcattgatt ataatgttcg tttataccat tttacggtat tgttcaaccc
    10861 ctgcggggtt gcgtggtgtt tcgtgttttt atggcacagg atataatcct gagttattca
    10921 ggttcaactc ctgacggagt tggagaggat tcagggtcag gtttttaggc tcaggatata
    10981 atcctgagtt attcaggttc aactcctgac ggagttggag aggattcagg gttaggtttt
    11041 taggctcagg atataatcct gagtttccat aatcccgaag aatagatcta agtttgtttg
    11101 catattcctg ttcatgtagc aatttcacta acttttcctg aagttgtata gtttcaagac
    11161 atggtatctg tctttcagat aaaaaacctt cagaaggttt tatatattga tggacgctgt
    11221 gggattcccc gcctgaccga actcaatcgg gcagggaacc tacgaccccc gtcccgccac
    11281 tgcgggagcg atgctctgac cagctggatt aaacattcag ctctactaat attttccctc
    11341 ggatttggtg gacgctgtgg gattcgaacc tacgaccccc gccttgtaag ggcgatgctc
    11401 tgaccagctg agctaagcgt ccgttttacc taagagcggg caaatttacg caataaaatt
    11461 taaaccgaca tttattttct aatggcaagg ttgagaactt ctgacatttc ctttacaaaa
    11521 tcaaatcgca tctcattttt tatctgcttt gggatttcga ttaagtcctt gcgattttgg
    11581 gctggtaaaa ttacccggcg caatcccgcc cggtgagcgg caagaacttt ctctttcacg
    11641 ccaccaattg gcagaaccgc cccgcgtaaa gtaatttcac cggtcatagc cagattgtct
    11701 ttgaccttcc gcttttttaa caatgaataa accgccgtta aaatcgttac tccggccgat
    11761 gggccatctt tcggtattgc gccagccggc acgtgaatat ggatatcgat tttcgagaaa
    11821 gcttcctcat cgagtcccaa taaagtcgca tttgagcgga tatagctgag tgcggcttca
    11881 gctgattcct tcattacatc accaagctga ccggtgagat tcaacttgcc ggtgcctcgc
    11941 atactggtgg cttctacaaa aagaatatcg ccgccaaccg aagtccaagc caacccgata
    12001 gcaacgccag gctttgagag tctttcagct atatctacaa aatagcgctg tggaccaaga
    12061 aaattttcaa cagctttttc atcaattaga aagttttttt tgcgtttatt aacagcgact
    12121 tcacgggcga ttttgcgaca aacgttggca atttctcgtt cgagattacg aactcctgct
    12181 tctcgggtgt agctattaat aataaaacgt attgctttat cagtaaactc aactgtgaca
    12241 ttagttaagc catgttcctt aatctgtttt ggaattaaaa aagtcttggc aatgttcaac
    12301 ttctcttctt cgatatagcc gctaaattcc agtatttcca ttcgatccct taaggctggt
    12361 aaaatagtgt ccaccatatt ggcagtggca ataaacatag ttttagagag atcgtaggta
    12421 acttcgaggt aatgatcact gaaagtcgaa ttttgctcgg gatcaaggac ttccagtaaa
    12481 gcagaactcg gatcaccgcg aaaatccgca ccgattttgt caatctcatc cagcataaaa
    12541 atcggattgc tcgattccgc tttacggatg ctttgcaaaa tacggccggg caaagcgccg
    12601 atataggtgc gccgatggcc gcgaatttca gcttcatccc gtactcctcc gagcgaaatg
    12661 cgaacgaatt tgcgacccat agcgcgggca attgacttcc ccatcgaggt tttgcctaca
    12721 ccaggaggcc caacaaaaca aaggattggg cctttgatac tggcgttggg atcctgttct
    12781 tgcttcaatt tgcggactgc cagatattcg ataattcggt ccttgatttt ttccagacca
    12841 tagtgatctt cgtcgagtat tttctgcgca cttttaatat ctacattatc cgttgtataa
    12901 atgccccagg gcaaatctac caaccagtcc agataggtac gcgaaaccgt gtattctgga
    12961 gaagccgggg gaatttttct aagccggtcg agctctttct cagcaacttt acgggcttca
    13021 tcgctcattt ttgataggtt aattttttct tctaattctt ttagctctaa ggtgccttcg
    13081 tcttcgccca gctccttgcg aatggctttc agctgttccc gcagaaaata ttcgcgctga
    13141 gttttggaaa tttcatcttg aacctcagac tgaatttgct cccctagctg gatgcgctgg
    13201 atttctcgat taagaattac ggtagctttt tccagacgtt tacggacgtc caattcttcc
    13261 agaatttcct gtttttcact aaccggcaca ttgagcaagt gaattattcg gtcaattaga
    13321 cggccgggat gctgaactga ggaaaatacc gaagcctgtt cttcgctcaa gtaaggggcg
    13381 attttaatca atttttgata tagttgacgg aggttagcca gcatcgcgtc tatttcgatg
    13441 cctggctgat aaatttcctg tgctttgagg atggctccct taaaataagg ctcaacctga
    13501 ataaatgatt gaattttggc gcgttctagc ccctgaacaa ttgcactttt actgccatct
    13561 ggcatattga atatttttaa tacctgggca atcgttcccc attcatacaa atcatctacc
    13621 tctggatttt ctaaagtacc ttctttttgg gcaaccacaa cgattcgttt tttagtttcg
    13681 ggtaaatcct ctattaaacg caatgagcgt tcgcgcccga tataaatagg gatgacttgt
    13741 tgaggaaaaa gcacggtgtt gcgcagagcc attatcggca atatttccgg tacaccatgc
    13801 cccgtctcat ttttttcatc tttatttttt attgtttcag ccatatttaa tacctttcaa
    13861 ataaacactt tatgtcatta tccaattcgg tctatttcac ttagaaccac cataactcta
    13921 aaagttacag cataatcgcc ttctttggaa cgtccggcaa tcgctggcca gagaccggaa
    13981 ggcaattgct gaccgataag ccagtccaac gcctttttaa agttaggatg agaatggcaa
    14041 tttggcaaag gggtcagggt ttccagaaac cgcaatgtgc cgccgcggaa taagccgttt
    14101 tcagaataat caatatataa atggttccag gcatccggct cggggaaaag cgtcgtgtga
    14161 ttctgctgta aatacacatt cagcaaaaaa tttgcggcac gctggcaagt ctcggtgtta
    14221 cgtaaacggg aatgagcgct gaaagcctga gtaattacca gagtagtcca tattccactg
    14281 cgagcagttt tcgccgattc gcctgtggct agcatagtcg gcgagagcca accgccatct
    14341 tctctttggc gctttgtcag ccagcggaaa cccttttcga caaatggatt accggctaaa
    14401 ccgtacctaa ccaataacca gagagtcaag ccctgatgat gttgaagtaa aggaaatttt
    14461 ccatccggtt tctgatactt caaaatagcg attaagcctc tctgaatagc tggcatatct
    14521 ttggtgcaac cgtaatccaa taactgcatc atattctgca actgtaacag aaactgcatt
    14581 gatttctgtt gctcttcgat tttgtagttt ttgttcagtt tccaaagacc gtcttcctgc
    14641 tgagtagcca gcaggcgttc ccgcggtttg aagaattgca gttctcgttt aagtcgctcg
    14701 accaattcgt tatcatgaga tagaaaacgc tcagcggtaa aatatcggat tggaagggga
    14761 gcttgctcca taagaatagg aataggatcg atttttacta atttagttgg gtgttttttc
    14821 atataggatt ctaagccttt atcatattaa aaagtgatct ttaagcgttt gctacggata
    14881 cattttgtat taattgttgg atttaattgt tcgggcaatt tcgctaaaat cgccctttta
    14941 taaatgggtt ctgtataaca gatgagtctt gttctcattt taaatatcga aaaaaccaaa
    15001 aagcgcctta atatcctctt aatcggcttg aaatgcaagt attttttagc ggatcaaaag
    15061 tcatagtatc caatttatta gtactctaaa ctttccctat gagatatctt gcttataatt
    15121 ttatatttgt caaattaatt ttcattgaat ttatctaaac atataatcgg aataattgaa
    15181 tatgaaaaat cctacttatc ataggtcatt ttcatttaat cgggttaata tagtttaaac
    15241 ttctataatt cagtcgtcgt taattcgatc gagaaagtag cagtttcttc cgggttcaat
    15301 ctgacatcga accacggaat tattactgaa ctttgataaa tgcgctcgaa tccggcttcc
    15361 gagagcgaga ctgtttcgat cgggaagcgc cagaattcga ccgcctgggg cgcaattact
    15421 tcggcttgga atttgtccca ttcattaaca actcctaaag ctttaacgcc agttacaacg
    15481 ccactggaat tcaaagccgc ttcagcgggc ttttcaccgt ccaagatata ataacggtcg
    15541 ggcgaattgc cgcccagaag agcaaagtta aactccggtc cgaagcggaa aacattctca
    15601 cgttcgccga tattggtcaa tatgtaggta actttcagtg cctcttttcc gaaagtaatt
    15661 gcctttttga tcctgaatct ttgccaattt acccagccgt ctttggagag agtcaacgtc
    15721 cgggtttttt cattgagttc tggagagtaa cggccggtga taaaatcgct gtcttcgtaa
    15781 tattcgccgt cacggaagcg ctccagcgaa acagacgggt gaatcacgtg atccagtaga
    15841 ttttttcgtg gatatttatc atacttgagg tacttatcca gtcccttttc cttggaaagt
    15901 accagatcat ggatactgcc gctggcattg gaattttcta ctttgtcggc taatgccact
    15961 ttgcggtgat aggattcgtg ataacgctga atattattca aaagattgaa ggctttgttt
    16021 accagcgata actccgttat catgccgcca cgatgcggtg caatagcagt atgaaggttg
    16081 ccgtatttta taataatttc attttcaccg tcggtatcga aatcaaacac ctccgctcgg
    16141 aagccttttg cacccgtgat tttatccagc agccgttcgc agattagcag ttcggtataa
    16201 attgcatggc gcaggtgcgg cagataaagt ccgccgaaaa taccatgcca gtaggcgcag
    16261 ttacaggtac tacgccagag atgcagacgg gcttgtccca attcaacttg ctgaggcttt
    16321 gttaggctcg tcggatcaat atcggcaaag cgttccgaaa ggtgcaacat tttcttctgc
    16381 atccagttgc tttcgtcgta tttatagaga aagttgcgcc agatgccacc tttgataaaa
    16441 ggtcggactt cctcaatacg cttcttgctt tcgaattcgt ggactaaatc ggcaaatttt
    16501 tcgccagcct cccacggcag agcccactca ctcatttcaa agtaggaagc cgttggtaga
    16561 taaatgcggc ctttgggcgg atgattttcg taccactcgg caaaggtcaa agtcttgagc
    16621 cactcgctat ttttcagcaa agccgcgaaa aaatttctca gccagccttt ggtaaaaacc
    16681 tgctcgtgag ttccgggcca gacgccgaat ttttcgccgt catccgccat aacaatgaca
    16741 ttttcaccgt cgtcggtggc gaatttagcc agatgatcaa tagttgcctg cggatcctgg
    16801 aatggaatgg tatagcgcaa ccgctgactg attgggaaaa taccgagggt cttgccttgt
    16861 tcctcagtca gaaaatggcc agtcagttcg ctttcctgtt taccattgct taaaaaatga
    16921 taatcgtcaa cggtgatgta ttttattcca gcttcggcaa ttggtttggc gagatgaggt
    16981 tcccagactc gttccgttag ccacagtcct ttagccgtat aattaaaacg ctgttgaata
    17041 taatcattca tcattttaat ttgaccgact ttatcggcgt cgggaatcat agccagcacc
    17101 ggttcgaaaa atccaccgga gataatctcg acgtttccgc gtttaaccag ggcggcaatg
    17161 cgatcgaggt aatccggatg atgcgcctcg atccattcca aaagacaacc gctgaagtga
    17221 aaggtgatgt ggatagcgct gaattcttcg acgatttcga taaatggcaa ataactttgc
    17281 cggtaggcag attccatcac gaaatcgaag ttacctaccg gctgatggtt atgaatgccg
    17341 aagataaaat ttacggtttt caaatgtatg ctattgagat ggctttattt taaaaggccc
    17401 atttttacaa ttaattcggc cacccgacag ctgtaacccc attcgttatc gtaccaggag
    17461 atgatcttag cgaaattgcc gtcaaggact ttggtcattc cggcgtcgaa aatcgaagag
    17521 tggggattac cgacaatgtc aatgctaacg ataggatctt cagtgtattc gagaatgcct
    17581 ttcattgggc cttcagcggc ggctttcata gcggcgttga tttcttcttt ggtggctggt
    17641 ttttccagtt cgcaagtcaa atcaacaacg gaaccatcgg gagtcggaac gcgcatagcg
    17701 aagccgtcca tctttttgtc aagagccggg ataacgaggc cgatagcttt ggctgcaccg
    17761 gttttggtcg ggataattga caccgctgcg gcgcgcgccc gacgcaggtc tttatgcgga
    17821 aaatcaagaa tgacctgatc attcgtgtaa gaatgaattg tattcattaa accgcgcttg
    17881 atgccgaaat tgtcattcag aactttagca accggagcaa ggcaattagt cgtgcaagaa
    17941 gcattcgaga cgaatattga atttttcgtc agcatttcgt cattgacacc gggaacgata
    18001 gttacgtcca catcttcctt ggagtcagcg ggaacggtca ggataacttt cttggcaccg
    18061 gccgtgatgt gtttttgcat gccttcacga ttgcggaatt tgccggtggc ttcgacgaca
    18121 atttccacac ccaatttacc ccacggcaat aaggagggat ctttctgtgc gaaaacctca
    18181 atcttttttc cgttaataac taaataattg ccgtcaacgc tgacggtacc atcgaatttg
    18241 ccatgaacgc tgtcgtattt tagcaaatgg gctaacgtct tagcgtcggt caaatcattg
    18301 atcgcgacga tatcaacctg aggattcttg taggaagcct ttaagacgag acgtccgatg
    18361 cgaccaaatc cgttgattgc gactttaatt gccataatta tactcctttt gttttatttt
    18421 tcaatttgtt cggatgattt ttaaatatga ttattaattt tcaaaaagcc gacctgaaat
    18481 ttaattttcc agcaatttac ttccaaaaaa attgtgggaa atggacttta attctttaga
    18541 aattccgcca attctcggga aatcacaaat accccagcct atttttccat ttcctcatca
    18601 atattttttt gccaataact tgttttctga tttattactt ttcaaaaagc gccttatttt
    18661 cattttatag aaccgccaat caaatcattt agccgtcaaa atttaaaaac aagagaaatg
    18721 accgaaaatt atgtcaatcc gatctggaaa acagctgata aaattcattc aaagtcaagg
    18781 ttggaatgcc ctcagtttca gcctggcgga tttgttcctg attcgtatat ccccaaccgg
    18841 ctataaacag gcgaattttt aggggcttga gtcttattaa agtatcaacc tggtcgtcaa
    18901 cgaaacaaaa attgcctggc gataaaccga ttttttgtaa aaggtcggca attatttccg
    18961 gtttaccttt ttggctatta gccgcgaata aatttttccg ttgaagagga gtaccggccg
    19021 tgtctaaaat tacctgaaca tattcgagtt tcttggtagt aattatgtat aaatgttccg
    19081 gcggtgagaa atttttgagg aaatccgcca ttccctcata gaacggattg agagccagcc
    19141 agcgcagtgg ttcctcgcgc agaaagcgag cgcgttcgag gtaaaactgc gttctgaatt
    19201 tttctttgac atcaatattt ttttcaatat agtcatcgaa atcagcctga ttatgaatgg
    19261 aaaagtcatc cgcaattgct ttaaatatat agacataatc ctcaccgtgc cgaataaaat
    19321 tgcgtaagcg gcgactctct tgttggattt cgcgcggcaa ttgacttaga tcgttgatct
    19381 tttccgaacg gccgatggca ctttggaagg cattgtgtcc gacgactaaa cattccgcga
    19441 tgctatcaac gatgacgccg tcgaaatcca gcgccagaat atcataagct ggattaaaaa
    19501 gagatttagc gttggaaatt tgtgacattt tttagttact ttggtttaat tataatttca
    19561 tattcttttt agaagccgag gctactgcgc cttccgccgc gttcttcata ttttaggtca
    19621 cggagggttg gtgacttaat attgattagc agaagatatt gtttgccata gccggacggt
    19681 gtccagctga aagccaactg ccagcaatgc agatcgcggg ttaccgatac gctctggctg
    19741 acgatttttt tctcaatcag gttcatactg ttttgataga cgattttcca cttagaggtc
    19801 aaattcaggg tgagattagt agtcataaag agactttccg tgcgtttaag gggattggtc
    19861 tggctcaaag agtaacgcaa gttgaaattg gcgttccaga gttcacttcc ttgcttagga
    19921 gtactttcct gcaatttatt gatattcgtc tcgccgtaag acggcataat tatatttata
    19981 tcggtcgtgt ccggtaaaat gtcggaagcg ggtgtggtca tatcttgccg gggacttcca
    20041 aaacgtttac cagaaagtga aaagccggtc gaagcgctga cgcttgtcaa acgtggtatt
    20101 gggatgccgt tccaagtttt gttccattca ttgatgcggc ggttattttg gtaagcatag
    20161 aggtcgtgag ttgcgttcag attgagcgca attttcttgg taacctgagt tcggaaggag
    20221 ctcgaaatca ctgaccagtt tagactatcg gcggcaaaat tatagccggt cgaagcattc
    20281 agagtaaaaa gatcgagctt gcgagttttt tcgccttgtc ccagtttagc ctggaaaaga
    20341 ttactgagac tcatagacat tgtgcgggtt tcataagaag acaaggcgcc cagcatagta
    20401 ttagtcagtg ggtcgaaagt ccgggcggtg ccggttgtgt cgattccgcc aatgatatag
    20461 cccggatccc acccaaaaat ttctcggctg aagtcaggcc taaatgtcat actgaccgat
    20521 ggcgtaacga catggcgaac agcctgcaaa ccggcaatcc tgacgggaaa caggccataa
    20581 gctttggttt gggcactgat tgtggcgcta ccggtgtgac gggctttaaa cccttctacg
    20641 gtttcggttt ttattttacc gttttccagc tgaaagcggt ggtcttcgtc ataaagcggc
    20701 atatcgtact tgaaaaccca gccttcatcg aggttgacag tttgattagt agtcaaaaag
    20761 ccgaaaattt tctgggaact ggtgaatgag aagttatatg taacggcatt ttttgcgatg
    20821 ttctgctcct gccattccaa tgaatcagta gcaggattat aaatgctctg gtaaaaaata
    20881 ttctggttat tttttagata ggagctggcg ctgaagtaaa tattattata ccatttagat
    20941 cgagaaacat cctgatgcgg cttgagggga atcaaaggct tactgcttcg ggtgaaagaa
    21001 atattcggca atgaacgact aatgtgcgca atggtcaagt tggaatcggt cggttcgatt
    21061 tgactgagag cattggcttg caggttagtg gtttgattaa aattaactga caatgaatat
    21121 ggtttccccg tccatgattt ggagagcgtg gcattgctaa tcaattgctg gtcgaggcgg
    21181 tcttccagat catagctgta ttttttatat agactttcat tgctgacaaa tttgccgttg
    21241 acgtttaggc gagtggttgg attgatcgtt tgattgtgag taatattgaa agcccattgc
    21301 ttctcaggat agttgctgaa aaagtcattg gtgtatttgc cgctgatggt gccattcagt
    21361 ttataacgcc aggcatagcg taggcgatag tcgagaataa cgccctgttt atcgtaaaaa
    21421 tcaccagtca gacggaaatc gtaataatcg ttcggcgccc aaaaatagcc cagtcctctg
    21481 atgaatccac cgccgattcg gctttcgcca taggagggca taatccagcc agaatgacgc
    21541 ttgccgctct gatccggaat aataataaac ggcaatccca aaagcggtat atcatgaata
    21601 taaaaaatta ccggccgagc gatcacttta tctttaaaga taatttttag ttgtttactg
    21661 cggaaataat aatgtggctt atccggaata tcacaagtcg tgtaaacgca atcattgacg
    21721 taaaagactt ttttatctac tttactgatt ttatcgccat aataataacc gtcctgttcc
    21781 ttggttttgc cactaacgat gcgaccgcgc tgagttttta aattataaac catgctttcg
    21841 ccgacaaacg gttcccgccc gcgctgatta agaaccggca aatcagcgtt ggtggaatcg
    21901 taaggtggcg ggcttaatgg ataagcatac aataaatttt cattccaatt gatgttaatt
    21961 ttgccggcag ttaactcgat atcggcgtac tgaattttgg cgtttagata aagatcggtc
    22021 agtttgcgat caacaaaata gtctatctgc tcggcgctat agataacggt ggtatccaag
    22081 tcggtgttgg tttcgtgcgg aataaagcgt ccctgcgtgc cgccgacaac ggtgattgta
    22141 ttgattttct ggtcaatgaa ttttaaccgg attgtgtcgc cgctgatttc attaatgccc
    22201 agaatgatac tgtcctccag catgttatac aggctggttg ccataacggc aaccacgatc
    22261 gaatcggctt gatcatctcg aaaataaatt tccatgaact tgccagtgat ttcatcgtaa
    22321 gttctgatag aatcaccatg agcgagacta tcggaatcag ctggcaaata agccttgccc
    22381 acggcattag cccgcgcatc ttcgtcaata aacagggcgg aaattttttc atcctcaaag
    22441 tacaagcgca tcagtttgcc ggtcaattcc tgaccgtcgc tcaaaacccg cggattatcg
    22501 gtcaacgtgg cgacttccag cgaatcaata taggtcaaag tctttgaata agcggtaaag
    22561 tccttactct gaatgcgcac gttgccaatt gcctgaaaat taccttcttt ttcaacgagg
    22621 cgaatggtat cggcgtaaat ccgaaacgat tcgacaccag cagtatcaaa acgggttagc
    22681 atcggtcggt tcaaggcaaa aatattgcca gtcttattga aataaatcaa actgtcggaa
    22741 gccagctcca agtgtcgctt gacatcgtat aaaacagcct gcccgatggc gactgatttc
    22801 ttttcgttag aataatagtg aatcaagtta gcacgaacgc tccgactgct atccttaaga
    22861 agaacattac cttgggcgtg agcgatttcg tctttgacta aatatatcaa gcgacgggca
    22921 gtgattgata aagtactgtc aaatagaatc ggacgaccac gggccacaat cgaatctctt
    22981 tcgctaaaat aaaccagcga atcagccttc agaactttat tatcactggt tgcaacgacg
    23041 ttgcggtaga aatcggcgcg ttgatctttt tctaaccagt aagccagatc gcaagccaat
    23101 tgcaacccgc ccttttggaa aagaacatcg ccagtaagcc ttttaattgg cacatcgaag
    23161 cggtaaaact gctcgagttt atcggcgcga atgagacgca gaggctcata cttttcggcg
    23221 gcgtcggccg aatcaaagaa caccaatcca atcagcataa ttagcaaact aattattaca
    23281 cccagtcgag taattttcaa gagtgattgc tggaaattaa tccttacact attccaatat
    23341 ctaccattta cctgaaaaca catcaatatt aaatataaaa attcttttcc tttgttctta
    23401 ggtttaatta atgatagcca aaaactatac tatcgtatta tgaatatttc attcaccgtg
    23461 agaataagaa taggttaatt tcgtgggata taattttcga tattctcgtc ttttgaccgt
    23521 aactatagtt tttgagaaat catttcacga atagttctta ctacggcgcc aactaataaa
    23581 atgttagatt atagtcaaag aagttattac attgctggca gttctttaat agaacaaggt
    23641 aatttggatt ttaacaagga gttgcagtta aggaggttga aatgaaaaag tattctgcgg
    23701 gatttggatt aattcttttg gtatccattg gttttgctca gacctatcat atcaccgatt
    23761 ttattccgga tttactgaaa acctggcagg tttatgggac gctcagttca tcgctttccg
    23821 gcgatgatag cgaagctgaa cgcaaagata ttggtactag cgaatacaca gacgaggata
    23881 aaaattcaac tataagtctt acgcccacag tttactataa atatatttgg ttgaccacta
    23941 atcaggaatt ttccattaat tctaatatcc aagggcttta tgaaagaagt aataatcgaa
    24001 gcgatcaaaa taattttaat cttggtttta gtattgacga tgtgacaaac aagtatattg
    24061 atggtagatt tcaaggaata atcgaatata ctcggtatct gttttgtcaa aatcggctag
    24121 gcgtttcagt cgggactaat tttaatttaa atgagaataa tcgcaagaca acggaaatag
    24181 aaaaaattca atcttcaatc actgaccatt gggataaaaa taatagcgaa tcccatcatt
    24241 ctcaccattc ggtttctctg tacccgggca ttgtctatgg ccgcatttac aatggtaatt
    24301 atgccgccaa agccgaagaa attttaaccg aattgaagaa gcagagtctt cttcagcgcg
    24361 atttgactgt tgccgaattt caggaattct cccatctaat aatgaatcat gttgcggctt
    24421 atcattatga ttcacgaatt aaaaatatcg aaactttaac agatattata gactatctcg
    24481 aatcaatcgg cgttctgaag gaactcgaca tagccagttt tgtgactatc aatgacatct
    24541 atcttttcag tccggcgcgt aaaatacgta atttcggtaa acaattttat cttcaaagcg
    24601 gactgagata ttcacccgat gtttataatt atgaagatga aaattggtat cgcagttggt
    24661 atgctgacaa tggcatcttc taccacgata gcctgctcga aaattatcgg acagttacca
    24721 gccagaagaa tcgagttttg tcgcattcgt actggtacaa aattggattc gaacattatc
    24781 aggtaaaatc atggcatttg tggtataact atggagctta ttttgatcac tcattttctc
    24841 cacaaagata tactgaaaag cttaagactg aacgtattga tattacaagc aggtcattta
    24901 acaggtcgcg tagcgatgag gttgcggcgt ttgccgggtt taattaccaa ttcactagcc
    24961 gatcggtcct aagcttaccg gtcagcatta cttattcaag agatcgcaca cattggaatt
    25021 caggatcaaa aaatatcgta tatttcaccg gagtgacaat gggagcggaa tttacctact
    25081 atctgacacc aaaatggtca ttgacttcat cgggccaaat cattcattct tattaccgtt
    25141 attctttgga taaacaaaca aaccgaagta cttatggtac attgagattt tcgatgacct
    25201 attattggta aatgctatag ggcaaggttc aatcacgact tacccggatt tttaccttgt
    25261 ttttatgcct ttataattga aaattaagcg taaatgacga gtaatttcag gagaaataat
    25321 atgaagcgtt ccacccttgt ccggtttgct attttctttt ctttgacctt agtgttaata
    25381 tttttctccg ggtgcgcgac ggaagcgcag agacattacg atctcggcaa atggtattac
    25441 gaaaaaggct tgatcaatga agccattctc gaattcaaag ccgctaccca gaaggatccc
    25501 gatttttatc aagcccatca gagcctggcg atcgcctacg ccaaaaaggg ctggtacgat
    25561 tacgctctga aagaagccga aatatccttc gatttacatc catcggatga aagctataat
    25621 ctgatccaga ttatccgaca taagaaaatt ctcgaaccgg cttacgagtc agccttaact
    25681 gatactcttc aatagtagat taaaaccacg actatttact accgggcgag gagcgattgc
    25741 ttccggtcgt taaatttgcg tcattttgaa agggaggatt tttaaaattt tatttcgtat
    25801 tctttgcgat ctttgcggta atttataccg atgaagcata ttctatccgt cagtcggcgt
    25861 agtgatattc cagcttttta tggcgagtgg tttttacaaa aagtccgcga aggcagtgtt
    25921 accttgccca gcccgttttc ccgccgaatc caggtggttt ctctgcgtcc cgaggatgtc
    25981 agcgcgatag tattctggtc gaaaaattac cagccctttt ggccgattct taagcagatt
    26041 aagccgcttt ataatggtcg ttttctgttt catttgacga tgaatggttt ccgcagttca
    26101 gcaaaaatcc tgctggaacc cggtgcgcct gatactgaaa aggctttgaa aacagctcat
    26161 ttattgtgtg atgaatatag cccggaacaa attttatggc gcttcgatcc cattattttt
    26221 tccaatctaa caccgccgga agaacgtctg gaggctttca gtaaattagc cgagcagtta
    26281 cgggggctga ctcggcgttg ttacgtcagt ttcgttgatc tctatggcaa agtcaaacgc
    26341 cgttttaacg ccgcagaagc ttccggcaaa atccagtttg ataagccgga actgcctgag
    26401 caagttgact ttctggaaaa tttgaaacca atcgctacca caaccggaat ccagttattc
    26461 acctgctgtg aagatgagat tggtgcggcg gctggtttgc cgaagggcca ttgtatcgac
    26521 gccgaattac tgaagcgcct ttttcccgat gaggaatttc caacccaaat gcgacctact
    26581 cgaccgcagt gcggttgtta tatcagtcgt gacattggag cctaccgcac atgccgtcac
    26641 gcctgccttt attgctatgc acaatgaggg gaacaaatat cactttttac tatttaatga
    26701 atagtttttt gattgatgaa tgaatagata tttttggtaa ttatgggaaa atttttgaga
    26761 tgattataga agagaaaatg cccattaatt tcgaaaatat agaaattaaa tgcgaatgta
    26821 attgacaaaa tagaaaaata accttatttt taatcttgaa tgaaaaatta aaaagtagaa
    26881 aatgagtaag acaaccaaaa cctccagccg cgattccagc cgaaccttga gtcggtatct
    26941 ggaagaaatc agccattttt caccgttgtc gcctgagaag gaaatcgagt tggcgcggcg
    27001 gataaagaat ggtgatcgtc aggcggcgaa ggaattgact gaagccaact taagatttgt
    27061 tgtcagcgta gctaaggact accaggggca aggattacct ctgatggatt taattaacga
    27121 aggcaatctt ggcttgttaa aagccgttga gcgtttcgat gagacccgcg gtttcaaatt
    27181 tatttcctat gccgtttggt ggatcaggca atcaattctg caagcgcttt ctgaacaatc
    27241 gcgtttagtg cggctaccga tgaatcgagt cggcactctc accaaaattg cccgggaagc
    27301 cgaagtgctt gagaaacaaa atgaacgctc accgcgctat gatgaaattg ccgccggtct
    27361 ggacgtcgat cgcgaagttg tgaacgacac cctccatatc gcgcgccgtc accgctccct
    27421 ggatgaacct atgggcgatt ctgatagcaa tagtctgatt gatgtactga cggatgagaa
    27481 tgttaccgat ccggattggc agttgatggt agaatcgcta agccacgata tcaatacctc
    27541 gttagccgcc ttgaataaac gtgagcgaga ggtgttgaaa atgtatttcg gaattggcag
    27601 tgaatatgcg ctgactttga acgaaattgg tgaacatttt aatctgacgc gtgaaagagt
    27661 acgccagatt aaggagcgag ctattcgtcg gcttagacat aaatcgcata gttcgttatt
    27721 gaaaacacat ctgggttaat tctgttttca atataagatt agaaaaatag tttctgtcgg
    27781 tgagtagagg ggaaattatt tatccagaaa gaagtatact agatctaaga ctggcaactt
    27841 gtgaacttgc cggattgata ctatattatt aaaagtatgc ttgattatta cctagttaaa
    27901 aatataaaaa tacctgacag agcagaagga atcgaaaatg ttgattgaaa gtctatcggg
    27961 ggtgcgcggt tatgatacgg acttgacgaa ggaactaata tcgtcgtatg ccgccgccct
    28021 aaaggaattg ctcgcctgcc gaagagtcgt ccttggacgt gatacgcgcg ccagcggcgt
    28081 tcgcatccgg gaacaattaa ctgaggctct gcggcgctat ggggttgcgg taattgacct
    28141 gggaatttgt cctacaccga cggtcgaaat ggctgtagtt caacatcgag ccgatgccgg
    28201 cgtcgtcata actgccagtc ataatccttt accctggaat ggattgaaat ttctgggagc
    28261 cgatgggctt tttcttgatc ccgagcagat gctctggatt aaacaaaacc gccaaagtct
    28321 tttagcgaaa tacagtgatg cccattttag tccgggcgat ttgatatttt atgagtgggc
    28381 aaatcgtgac catattaatg ctatcttgaa attaccgtat gtcgagatgg aatctattcg
    28441 tcgtcaacat tttaaagttg ccgtggatgc tgtcaacggt gcgggttcca aaataattcc
    28501 agaattactt aaagaatttg attgtgaagt aattgcgatt cactgcgatc cccatcagcc
    28561 ttttccgcat ccgccggaac ccttgccgga gaatctgacc gatttgtgcc aggcggtaaa
    28621 ggacaactcg gctgatctag gcttagcaat tgatccggac ggtgaccgct tagccattgt
    28681 ctcggacaaa ggtgtgccga tttccgaaga atataccctg gtgctcgcga ctaaattggt
    28741 attgtcaaag accggcaaga agaaccaaaa ggttgtcgct aacctttcga ctactcgagc
    28801 gttggaggat cttgccgctc aatatgatgc tgaggtaatt cgtactccgg tcggcgaaat
    28861 caacgttgcc cggcgtatgc agatggaaaa ggctttaatc ggcggtgaag gcaacggcgg
    28921 ggtaatttta ccggaagctc atctgggacg tgattcgctg gtgggaatgg ctttagtttt
    28981 gcaattgctg gcggatgaaa agcgctcact ttcagccgtg atgccgactc tgccacaata
    29041 ttttatggta aagcgtaaaa ttcctcgcgg gaaagccgat tttaacgaaa ttatgaatca
    29101 tctgattcag tctgttgaca atgccgaaat taacacagaa gacggtttga aatttagctg
    29161 gtcggatcgc tgggtgcata tccgcacgtc caataccgaa ccgattatgc ggatttacgc
    29221 cgaagcgcca accagtcagg cggctgatca attggctgat cgtttcgtca cattattgca
    29281 gaaatttatt taaagctaat atatttaaat taataaggaa attatcatga cttatgcctc
    29341 gataattaat cccgaagaag cgcgcctcgc tatcgcgcat ttaatagacc atactttgtt
    29401 gaaaccggaa gcgacgccga acgatatcgt tcgcctttgc gaagaagctc tggaatatca
    29461 ttttgcaaca gtatgcgtca acccatcctt tgtggaattg tgcgccaatt gcctctataa
    29521 ttctaaagtc gccgtagcga ccgtaatcgg ctttccgctg ggtgctaata cgatggaaat
    29581 caaagccgct gaagtggagc aagcgctaaa tcatggcgcc accgagttcg atatggtcat
    29641 caatatcggt cgcctgaaag ccggtgattt ggaatacgtc gaagatgaca ttcatgccgt
    29701 tgtggacgcg gctgatggta atttggtgaa agtgattatc gaaaccgcac ttctaactga
    29761 cgaagaaaaa aaactggctt gtcatctggc gctcaaagcc ggtgctcact ttgtaaaaac
    29821 ttcgaccggt ttcagtaaat acggggcaac agtcgaagat gtgcgcctga tacgaagcat
    29881 tgtcggtaac cagatgggag tcaaagcggc tggcggcatt cgcacttact ccgatgctat
    29941 attgatgatc gaagcgggcg ccaaccgcat cggaaccagc gccggggtac agatagttca
    30001 ggaaagtaaa cagtttaaga atcattgaat cttcgacgat gagcatcaga ttatttgagt
    30061 cggtgcggta agattggaat gagagcaggg agggctttat aatgttccta aatacaacga
    30121 agctcccatt ctttgaataa aattctctgt aacgaacaca gtaattaatc cttggaaaaa
    30181 acagttgaaa tgttgaaaat agcggctatg aatttactat aaaatgaaag gtctttataa
    30241 atgggtatcc caggactgac tattatcggt gaatcaatta acgattcggt gccttcgact
    30301 cacgatcttt ttcaggcgaa cgacctggat ggtatcgttg gactggctcg tttccaggct
    30361 gaaaagggag cggcttatat tgacgtgaat gtcggtcggc gttcacctgc ttttatggcg
    30421 gaggtcgtcc aaaaaatcca acagcacgtt tccgttcctt tatcaattga tacgccagat
    30481 ccggaaatcg ctctcgcagg tctggaagcc tacaatccta aactggctgg taatcgtttg
    30541 ccagtcctga attcaatttc agaagctcgt ctggaaattt ttgatttata ctccaaacag
    30601 cccttcattc ccattttact cattactgaa ggaaaaaatg agtcgggcga aatcgttatg
    30661 aataaaacac ccgaacagaa ttattccacg gcaaagtctt tagtagaaat tgcccgggaa
    30721 aggattcagg gagtagccaa taatcgcctt atcctcgatc cgggcattgc cccgatcggc
    30781 agtgatatgg agggcaattt caagagactg atgaaagtaa tggaagtgat ttatcaggat
    30841 cctgacctgg ccggtattaa tatgtcagtt ggtttgagca actttactgt tatgttgccc
    30901 tcaaaaaagt ccgacggctc gccggtgaaa agtccgctgg aaagcgcttt tttgacactg
    30961 gcgatgccgc tgggtttgaa ttttatcgtc ggttcggtaa aacgtaaata cgagattctt
    31021 ccggatgacc atccggcggt acaatgtttg aaaaatattc tggtatccga cggattaaat
    31081 gccgtcttgc aaattatgaa ctttatagcc tgattcttaa taaccgttaa gtcgaatttt
    31141 caaaaaggga aaatatggat ttagtaactt tcgataaacc gccaatatct gctcgcgagc
    31201 gcaagttatc acgcttcatg ctcttttttg aagaccttat aaaagtcccc gtatttcatt
    31261 gtcagcattg cggcgaatgt ctgctttcgt caactggatt tgtctgttct cagcgctgcc
    31321 ccaagcgact gcgcaatggt ccgtgcggcg gcacgcgaga tgacggccac tgtgaggttt
    31381 tccccgaacg caaatgtatc tggtatcgga tataccgacg atcaaggtta ttagggcgca
    31441 tcaaactttt gtataaaatt aatcgaattc acaactggaa tctggaacat acttccgcct
    31501 ggctgaatgt atttagaaaa cgcatcgaag gcttggttct atttgttcct aagaatttag
    31561 atatagagaa tattaatgac actcagagaa aaaattaatt ccggaaaatt tatcgtgtgt
    31621 ggcgagattg gtccgccgca gagctgtgac ggcgatgtga ttcggagaaa agctcagtat
    31681 ttcaagggaa tcgccgatgc tgtcaatatt accgataacc aaactgccat cccgcgtctg
    31741 tcgagcattg cttcggctaa aatattgctg gatgagggag ttgaaccgat tattcaaatg
    31801 acctgccgtg accgcaaccg attggcgatt caaagcgacc tgctgggggc ggcggctctc
    31861 ggtattcgaa atgtgctgtg cctgaccggc gattaccaaa aattaggcga taacccagaa
    31921 gccaagggtg tgttcgatct ggattcgatt caactgatag ccactgtagc ccaaatgaat
    31981 tcaggttatt tactctccgg tcgtgaaatg aaaaaagcgc cggaattttt gattggcggg
    32041 gcggcaaatc cttttgccgt gccttttgaa atgcgtctga ttcgtcttta taaaaaagtc
    32101 aaagccggtg taaactttat tcaaacccag ccggttttcg atccggagct ttttattcgc
    32161 tggatggaaa aagtagtaga gatgggattg cacgaaaaag tcgccattct agctggggtt
    32221 atgccggtga agtccgttaa caccttactg catatgcgag aagacgtacc cggagttaaa
    32281 attaatgatg aatatattcg acggatggaa aaagctgccg atccgcaggc tgaaggcgtt
    32341 agaatcgccg tggaaactat ccagacagtc aaaaatatca aggggattcg cggagttcat
    32401 attatgcctt ttatgtggga aagtattatg ccaactgtag tgaaagaggc tggtctatgc
    32461 aattaaaact aaggagttag ataatgcagg atattattga gaaaatcgcg ctctgtgtcg
    32521 aaaaggggaa aatttcacgc aatcacccct ttccgccaga tatgaaagat caagacggcg
    32581 ctgatgaaat tgccgccgcg gctttgaaag aaggcgtccc gcccgatgtt ttactgcagg
    32641 cttctatgct gggaatggaa cggattggga aaaaattcag cgaaaatctg gtttttgtgc
    32701 cacatttact ggttgccgct caggcaatga atgccgtctt gaaacatttg caaccttatc
    32761 tggaagccgg ttcggttaag cgcaaaggta aatttgtaat cggcaccgtt gctggcgatt
    32821 tgcacgacat cggtaaaaat ctggtagcta tgatcgtaag aggcggcggt tttgaggtta
    32881 ttgatctggg caccgatgtt tccactcaaa agtttttaag tgctgttgaa caacaccccg
    32941 gttgttttgt tggacttagc gccttactaa ccactaccat gttaaatatg ggaaaaattg
    33001 tgtcggcaat cagagaaact tttccgacta ctaaggtatt aattggaggc gcaccggtta
    33061 ccgaagaatt ccgtgcccaa atcggagcgg atttttactc acccaatccc caacaggcgg
    33121 tcgagtatct gaatcagttt gtgagctgat tttcataacg tttaattatt caccttgtcc
    33181 attctattat ctgtcagtat tcgatggggg tataagtatc aatgggcttc gtgatcttat
    33241 tcgtgatttt tcttcaactc ggtgatttcc cacggcaata tgatgcggcc gggtgtgttc
    33301 aattcggcgc gaatccgtat aaaacataat ccagcgtatt ggtcttggga ttgaatggat
    33361 ttgccccatt ccgtaaatgg ggtttcgcaa gtagtcattt tacggctgga agattcgcga
    33421 atgagcagtt ttccattttt aactactgag attagcatat gcgctgaaaa tatgtcgctt
    33481 ttgttggcga aaattaacac cagaatatcg ccgttgcaga tattattttc gactttaaac
    33541 agatattcct tgggcaaata ttcgagggta atagtacggt caggcttgac atagcgcaaa
    33601 tcggcaatgc ctttatcctc gaaaaatttg cgatgtaaaa ttgtgcgagt catttgttga
    33661 atgtattgtc cacccacttg atgtgagacg tcttccaata accagctgtt ttccggtaac
    33721 caatcagcca ttgtataatg attacgggtg cgcatgccga taatgccatc cttataccga
    33781 atttgctgga gattattgaa aaaattgtcc cagctatcag aaatcgagag cgccaaaaca
    33841 tgttcacaaa aaaccataca attagtttct ttgaaattaa ctaatggcca gggttcataa
    33901 agggcataag ggccatcgcc ggcgcaattt tgattgtatg gcataccgag aaatttatca
    33961 gagaagagat ttatgcgatc agtaattgtt aggttctggg tgctgacaat tgttaggagg
    34021 ctatcaattt ctggattgct catttgataa aacggctttc cctcacccaa caattcggaa
    34081 ataaatacac tgatgaccaa agccagtgaa attgtgatat agatagctct tttcatagat
    34141 agctccataa aattgcttca atagcggtcg aatttacagc actgattacg aaatgcaaat
    34201 gatggcatgt ttgc
//
LOCUS       DAOSMP010000026        29575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_1579_length_29575_cov_20.688245, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000026 DAOSMP010000000
VERSION     DAOSMP010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 29575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29575
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1579_length_29575_cov_20.688245"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PKX09_05860"
     CDS             complement(<1..734)
                     /locus_tag="PKX09_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOU17382.1"
                     /translation="MSIPLITIIILLIFLISSAIRVLREYERGVIFRLGRLIAAKGPG
                     LFFLIPVVDRMVKVSLRTVVMDVPPQDIITKDNVSVKVNAVVYFRVVDPSKAVVEVED
                     YLYATSQLSQTTLRSILGQAELDELLSSREKINAELQTILDTHTDPWGIKVSQVEIKH
                     VDLPQEMQRAMAKQAEAERERRAKVINAEGEFQAATKLAQAAEIMNKEPITIQLRFLQ
                     TLREVAAENNSTTIFPVPIDLFKPFV"
     gene            complement(756..2072)
                     /locus_tag="PKX09_05865"
     CDS             complement(756..2072)
                     /locus_tag="PKX09_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HOU17383.1"
                     /translation="MRSRFYLPKIFLILLVGSILLTATPKEIWVLEVDGVINPVSASY
                     INDNLRKAQPDQVECLIIQMDTPGGLMTSMRDIIKNILNSEIPVVVYVSPRGAQCASA
                     GVFIAVSAHFVAMSPGTNIGAAHPVNLGGGGIGGQPDSASSQTMIEKATNDAVAYIRS
                     LARERGRNEDWLEKAVRESASITETEAVKLQVVDVVVDDLDELLEILDGKTFKLPSGE
                     KTVHTQEATIRFRPVGIHHRILDVISDPTVAYILLMLGFYGIYFELSNPGAIFPGVLG
                     AIFLILAFFAFQVLPINYAGLALIILGIILFILEVKIISYGLLTIGGIVAMTLGSLML
                     IDVNQAPEILEAVSLKVILPIVIFTAAFVIISLTLALKAHKRKPTTGIEGMIGETGQT
                     ITDVDKTGMAIVHGEYWKVRSDTPIPKGTEIVVLDVDRMVLKVKPK"
     gene            complement(2136..2486)
                     /locus_tag="PKX09_05870"
     CDS             complement(2136..2486)
                     /locus_tag="PKX09_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17384.1"
                     /translation="MQFSIAGEIPHIYHIPPKTLIQNNPAEITCVITPGDSQLKWVKI
                     FLRDDSSELYKEFNMQYRDGIWFLPLIPEMVSGNYLDYFIIAEFEDFTALAFPPERPA
                     EKPIRVQIVSTKKI"
     gene            complement(2602..3927)
                     /locus_tag="PKX09_05875"
     CDS             complement(2602..3927)
                     /locus_tag="PKX09_05875"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOU17385.1"
                     /translation="MSKFCRYFFILAMLVFFLPMSISAQHFDQVMSSEALRPFWSIYG
                     IGASSFTLLNNSYLETDIGSTQSNPAFLINVKRPKGSFSGISITPKNLTTLADGGKDY
                     SASTTKYNVSYIGFAYPFPVYQGSFVMAASYSPAAYYYSSSYTKGTTGAAYYDEDGNA
                     HIVDTDLEYDINESGTLNVFRFAGATEFMKNFNLGLSFNFYSGQRNYESTEIGVDVND
                     ELDFNSIIYQENIKPSYSGFNMDFGMCYQSENYKIGLRLSTPLKLKVHEVSELTETYS
                     YDTVDSIFPNSYDFKYRTRYPLEIAPNFAIKVGKISLGMDFIFHNWQKIEVDLLDEMR
                     EVNRDLYWNLRNTTDIGAVLTIPFGNFVSTRLAYRLVPSPYAEYEPDEEYYHVMGAGI
                     ETILKESIIIGCSFQHGFGNLSNFYRYFETTTTQAYTENRCSISLAVLF"
     gene            complement(3936..5042)
                     /locus_tag="PKX09_05880"
     CDS             complement(3936..5042)
                     /locus_tag="PKX09_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17386.1"
                     /translation="MKANALTQIILMTAALIISSCYTQFSAPIVRQETYQQSVTDTTY
                     QEQETYENYTFDSSYLNDYRLGYRDGYWDSMNSQLWLGFNIYSPFYGGYRYHSPYLGY
                     YDPWFYDPFYDWGFYGYFSFYGGYYPYYGYYPHYGYYGYYYGGYPYHRHHWHGYRDGT
                     YYNEGRDELKADYVPKSTPSGSRGASNNASSQSGAAYLPTSTSAITTTSVSGEKTTVT
                     TTNSGSTKARYSPKTTGAGNSSQEKAVVGFQTTLVPTSAPKTTVITTNTNSRGSTRST
                     ASQKSSSPRATVRKGTSSSSGSSNTGNSSSSSSSSSGSSYQPASSSSSSSSSSSSSSS
                     SSSSSSGSRSSSSSGSSRSSSPKASGDSSKNNRK"
     gene            complement(5212..6945)
                     /locus_tag="PKX09_05885"
     CDS             complement(5212..6945)
                     /locus_tag="PKX09_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17387.1"
                     /translation="MKTVKLFILSLLALTFLTCESQKANDEPVFIFQSPKVLFKNLIA
                     NNLIVPRFSPDGLDLIFNGRIDGDLWDGIYQIPIVGGLPQKIYSTTDDILYPSYSADQ
                     RQVIFSQGLARQIYLLDIVTKNVTPLPIYGNYPTILPDGSTILYTGGLDGNIRLYDLR
                     ENRHRVLTKSHSGINIFPMMTQDRVGFIWLENRKPEQVRVNQTDFDALKTKILQIFAE
                     PLLSWTISPSGEWAIASRPNGEPFGFKLYDTAFASISIQPDTLVKNVKHLAFSVDWSP
                     TGRNVVYVGNTVPQFSRENPFTYRGIFKGDLVISNLRWENIEEAELLKSPAVSNWTIF
                     PYSEPNIQGSPRFVPTNINNPPVITSVPVETARQGELYFYRVQAVEIDLFDKITYSVV
                     SGPENATILKNTGILVWQPIDSGRFEFTVAVQDNYDGIDSQTFYVDVLPSKKGDDIFY
                     QSQPVDKKASGFAASLSFLDSNGDGFLMPGEEAALQIDLKPLYAEKLDSVRLQLLCSA
                     ESREVSYDRELIFKDCQPNQWNQMIVPIKALPKLQNRSIVFWGTIETKYGFQILPASL
                     IINGKNPEKNI"
     gene            7260..8708
                     /locus_tag="PKX09_05890"
     CDS             7260..8708
                     /locus_tag="PKX09_05890"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOU17388.1"
                     /translation="MYIILVKRSFFKKIGRLCWFSTGWIGARYIYRELIFPFLFSIGV
                     VTFILLANFLIKSVDRLLGKGLPLGVIFEFLYLNLAWIVAMSVPMAVLVATLMAYGRL
                     AEDNEITAMRSSGISFTTILVPGFIFGLVVSVVMLYFHDGVLPEFNHRARLLTSDIYR
                     KRPDLNIEPGYFVDDLPDYSIYVKEKVGESLRKVTIYNKDARDIQTTIYADSGYIGVE
                     GNTILFTLFSGQIHEIDLKGLSDYRRIDFERHRIAIPVDNLTLERRESERRGDREMTI
                     KMMRAEVERYSREYQQVIAKINNLVRKELGDTLTSFDLKKIEKKVQTLQAENLKNLPP
                     AEAARRNQKLTIFLNRIRSESNLAFNYKKQIAKYNVEIQKKYSIPFACIVFVLLGAPL
                     GVITRRGGITVSAVLSIFFFLLYYVFLIGGEELADAAIISPFWAMWTPNLILLIVGIY
                     LIYYTSSEQRVIDFSRFPYLFRRLNKKIEKNP"
     gene            8728..10065
                     /locus_tag="PKX09_05895"
     CDS             8728..10065
                     /locus_tag="PKX09_05895"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOU17389.1"
                     /translation="MPSVKQMKRTKNFIMVDLIEKKKAGLEHSKEEIQYLIASYTKGK
                     IPDYQMAAWLMAVNFQGMTDAETFALVETMLLSGTKLDLSHIKHCKVDKHSTGGVGDK
                     VSLILAPLAVAAGCVVPMISGRGLGHTGGTLDKLESIPGFNVNLSSQEFVRLVEKYGL
                     AMGGQTEELVPADRKIYALRDVTATVNSIPLICGSILSKKIAEGIDGLVLDVKTGNGA
                     FLADYNQSQQLAKQLKRVGENFGLQIKAVITDMNQPLGRAVGNWLEVVESIVTIKGQG
                     PEDLTAITLDLTAWMVVLSGIEKNFGEARELLKQLIISGEAYQKFLLMVEAQGGYISY
                     IEHPEKYKQAAWQASVLATESGWVKNIDTKRIGMCAVALGAGRQSLDSVIKPEVGLII
                     NKKIGDYVEVGEPLVTVHADSRRAFQNVQSAIRKAYHFSKAPVTPPPLFYETF"
     gene            10167..10853
                     /locus_tag="PKX09_05900"
     CDS             10167..10853
                     /locus_tag="PKX09_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HOU17390.1"
                     /translation="MNYPKSKRSSGILIAAEGLDGSGKSTQLYLVKRWLELSGHHVYL
                     TEWSSSEMVRQAIKRGKKKNLLTPTTFSLVHAVDFADRYHRQIQPLLKTGFITLSDRY
                     IFTAFARDAVRGCSSAWLRNLYSFAKIPDITFYFKLSPQIAAERILSNRPKIKYYEAG
                     MDLNLHPEVYDSFRIYQGKIGTEYDQMSKEFGFVEIDATQPIKTQQEKIRAIIAEKID
                     LASYKKGELE"
     gene            10850..11530
                     /locus_tag="PKX09_05905"
     CDS             10850..11530
                     /locus_tag="PKX09_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HOU17391.1"
                     /translation="MKSFYLEPLPKCDISRLSGKLIVLEGSDGSGRTTQIARIKSWLE
                     GKGYAVVNCELARSQLVGPELELARRGNTLCPTTLSLFYATDFMDQLENVIIPALRAG
                     FIVLADRYFYTLMARDLVRGTDLKWVQNIYGVALVPDLVFYLNVSPRRLIERRWQKAN
                     AMDYWESGMDMGLSRDWAVSFLIYQQYLRKVFLKLQDMYGFIVINGNKSITAITNDIV
                     KQIEKVLI"
     gene            11746..12600
                     /gene="hisG"
                     /locus_tag="PKX09_05910"
     CDS             11746..12600
                     /gene="hisG"
                     /locus_tag="PKX09_05910"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOU17392.1"
                     /translation="MITLAIQKTGRLNEHTTKLLSECGLFVGNGSTARLMTEASNFPM
                     RILFLRDDDIPECVADGLADLGIVGENVYHEKARPLKIVERLGFAKCRLAIAVPKDFK
                     YEKLTDLNGLSIATSYPNILRRFLNENNLKAEIHEISGSVEIAPGIGLASAIFDIVST
                     GSTLLTNGLKEVVTVLKSEAVLVASENLSPEKTDLLEQLLLRIRAVQAAHTRKYILLN
                     APDSAIEKICAILPGMKSPTIMPLAEPGWSSLHSVIEEHQFWERISALKAAGAQGILV
                     IPIEKMIM"
     gene            12610..13911
                     /gene="hisD"
                     /locus_tag="PKX09_05915"
     CDS             12610..13911
                     /gene="hisD"
                     /locus_tag="PKX09_05915"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOU17393.1"
                     /translation="MKVIKYPQRTSWPELIRRPAIDNKILRNLVIEIIEAVKTDGDDA
                     LRQFSWKYDNVRLKEIEILRDELASVPNEVDDDLRKAIEIARANITKFHSSQFEGIEK
                     VETTPGVICWRKSVPIESVGLYIPGGSAPLFSTVLMLGIPARLAGCPEIILCTPPNKD
                     GRIHPVILYTAQILGIERIFKIGGAQAIAAMAYGTQSVPRVAKIFGPGNQYVTIAKQI
                     VSAQGVAIDMPAGPSEVAVVADRFANPEFVAADLLSQAEHGADSQVICVTDNEELIPQ
                     VITAVKQQLAELPRRTIIEKSLESSRLILVNNLDEALEFINCYAPEHLILQTQNPEQW
                     ADKVINAGSVFLGDFTPESAGDYASGTNHTLPTNGYARNYSGISVDSFVKKITFQQIS
                     PAGLKAIAPAIDCMATTEKLQAHRNAVRIRLEAIEKGAGRG"
     gene            13886..14950
                     /gene="hisC"
                     /locus_tag="PKX09_05920"
     CDS             13886..14950
                     /gene="hisC"
                     /locus_tag="PKX09_05920"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOU17394.1"
                     /translation="MKKELDVVDLEKLARRNIINLQPYATARDDYSGAIGIFLDANEN
                     SLGSVIPQKFNRYPDPHQKRLKQEIAALKNVNPEQVFLGNGSDEVIDLLIRVFCQPGT
                     EKIMILPPTYGMYAVSAASNDVGVVSVALTPNFQINLPQIFEQIAGVKLIFLCSPNNP
                     TGNCLNPADVVNLLEKFKGIVIIDEAYIDFAPQKSLLPRLKEFNNLVIMQTFSKAWGL
                     ANLRLGMAFADPQIIDWLSRLKYPYNVNGLTQELALSALERSAEKDRMVAELCTERER
                     LAGSLEELPIVEKVYPSDANFLLVKFRDARSVFQYLINQKIIVRDRSQALHCENCLRI
                     TIGTPNENDILIAKLKDFAR"
     gene            14947..16038
                     /gene="hisB"
                     /locus_tag="PKX09_05925"
     CDS             14947..16038
                     /gene="hisB"
                     /locus_tag="PKX09_05925"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HOU17395.1"
                     /translation="MKKVLFIDRDGTLIIEPPDEQVDSLEKLEFLPGAITNMNKIATQ
                     FDYQLVMVTNQDGLGTDLFPEKDFWPAHNKMLQILAGEGVVFAEILIDRSLPNDNAPS
                     RKPGIGMLTDYLKGDYDLENSFVIGDRETDVQLARNLGTKAIYLGSNESISADYKAKN
                     WSEIYNYLSNISRTATVTRKTNETDISLVINLNGKGANRIDTGIGFLDHMLNLFATHS
                     GCDLDLTARGDLAVDEHHTVEDVALVLGEGLLKALGDKKGIERYGFLLPMDESLAQVA
                     LDFSGRREFVWECDFKREYIGKMPTELFRHFFKSFADSAACTLHIKATGENEHHKIEA
                     VFKAVGRAVKMAIRRDPTCDQIPSSKGKL"
     gene            16035..16616
                     /gene="hisH"
                     /locus_tag="PKX09_05930"
     CDS             16035..16616
                     /gene="hisH"
                     /locus_tag="PKX09_05930"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOU17396.1"
                     /translation="MKVVIIGYNSGNIHSVRLALQRLNVKAVVSSDPAVIQAADKVIF
                     PGVGEASSAMESIKANGIFEILPRLQKPTLGICLGLQLMCRDSEENATQCFGIFDIAV
                     RKFPPLAKVPQVGWNTLFDLKGDLFRDVAENSYVYFVHSYYAELSEHTVAQTEYIIPY
                     SAAMQKDNFFAVQFHPEKSGKVGEQILRNFIKL"
     gene            16613..17335
                     /gene="hisA"
                     /locus_tag="PKX09_05935"
     CDS             16613..17335
                     /gene="hisA"
                     /locus_tag="PKX09_05935"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOU17397.1"
                     /translation="MITIIPAIDIINGKCVRLSRGDFNTQKIYNENPLEIARQFADAG
                     IKRLHLVDLDGARQKRVVNWEVLEKIATGTPLQIDFGGGIRSETDVRIAFESGARQIT
                     AGSIAISAPDLVEEWLKKFGGEKIILGADVIDRKIAIHGWQEKAPLEIMEFLQNFQEK
                     GIQQTICTEVARDGMLTGPAFELYREIKQIFPDLYLIASGGISRIDDIYQLQDDGIDG
                     VIIGKALYEGRLKLSELKGFLC"
     gene            17329..18084
                     /gene="hisF"
                     /locus_tag="PKX09_05940"
     CDS             17329..18084
                     /gene="hisF"
                     /locus_tag="PKX09_05940"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOU17398.1"
                     /translation="MLAKRIIPCLDVKDGQTVKGVNFINIRRAGDPVELGAAYAEQGA
                     DELVFLDITASIEKRGTFIELVREIAKHLNIPFTVGGGVRNADDVARLLDAGADKISI
                     NTAAVNHPEMIEKLAGRFGSQCIVIAIDTKHTENGDFVYINGGRTPTNQTTQAWARQV
                     VELGAGEILLTSMDHDGTKRGFALEITRELAENLPIPVIASGGAGTMEHFWEVFKIGK
                     ADAALAASVFHFGEIPIPELKKFLKSNGIEVRL"
     gene            18081..18677
                     /gene="hisIE"
                     /locus_tag="PKX09_05945"
     CDS             18081..18677
                     /gene="hisIE"
                     /locus_tag="PKX09_05945"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOU17399.1"
                     /translation="MNIDFTKNNGLLPAIIQDEKTGKVLMLGYMNRESYEKTKATGRV
                     TFFSRSRQALWTKGETSGNFLEVKEILSDCDGDTLLIKVKPTGPTCHTGADTCFNEKN
                     IAEKPFIFELEEIIRDRRNNPKPGSYTNKLFDAGDERIAQKVGEEATEVVIDAVTGNE
                     MRLPEEVADLLYHLLVLLVAKDVALDDIMNVLEKRHRK"
     gene            complement(18851..20860)
                     /gene="ligA"
                     /locus_tag="PKX09_05950"
     CDS             complement(18851..20860)
                     /gene="ligA"
                     /locus_tag="PKX09_05950"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOU17400.1"
                     /translation="MNLDALREKAKELRRQIETANYNYYVLDNPTISDAEYDRLMREL
                     EQLEAAYPELRTPDSPTQRVGAQPLDEFKTVTHRVPMLSLDNAMDEGEIRDFAIRVQK
                     SLPGETVQFVCEPKIDGLAVELVYENGVFISGSTRGDGITGEDITQNLKTIRSIPLRL
                     MPNLPIPTLLEVRGEVYMDKADFQRLNEQQLKEGKPLFANPRNSAAGSVRQLDPQITA
                     KRPLKIFCYGLGALEGYSFQTHHEFLEALLKWGFRVNPLIEIAMEFEELIAYYHKIES
                     IRDSMPYDIDGVVYKVDSLAQQAKLGIKSRSPRWAIAGKFKAQQEVTQIIDIEASVGR
                     TGAITPVAHLKPVQIGGVTVSHATLHNQDEIDRKDIRIGDWVVVQRAGDVIPQVVKVI
                     TERRTGNEKRYSIPANCPVCGTPVVREEDEAKHRCPNINCPAQIKASIEHFASKRAMN
                     IEGLGEKLIEQLVDSGLIKNVADIYSLRKEQLLTLERMGEKSAQNLLDAIAASQNVSL
                     ARFIYALGIRNVGEHMAKVLAKAFGSLERLSQASEEELMAIEGVGPIVARSVRNFFNA
                     ESNRQTIEKLLSSGIRISTEPDYKQKGEIAGKTFVFTGTLANFTREEAQALVEKYGGK
                     SASSVSKKTDYVVVGENAGSKAETARQLGVKILNEAEFLELISYN"
     gene            complement(21031..21438)
                     /locus_tag="PKX09_05955"
     CDS             complement(21031..21438)
                     /locus_tag="PKX09_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17401.1"
                     /translation="MQSMKKIFLIWMFSTIAFAQQAVEVSDIIISDLDNNLIEIRVEV
                     KNTSMKSVSELAGFIDIYDKYGNVIEKQEIVIVLNSDIPLKPQNTASGSIIITQRPNM
                     SGQVNFRITNLRYFGEPNVYLICPVCGELIPKD"
     gene            21856..23319
                     /gene="guaB"
                     /locus_tag="PKX09_05960"
     CDS             21856..23319
                     /gene="guaB"
                     /locus_tag="PKX09_05960"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOU17402.1"
                     /translation="MNEKIAREGLTFDDVLLIPSESNVLPKEVSLDAKLTKKIQLNIP
                     ILSAAMDTVTEGKMAIAIAREGGIGILHKNMSIEQQVAEVDKVKRSESGMIVKPITLS
                     SDSTIRDALNLMDRYSISGIPVVDNGQLKGILTNRDIRFETNLDLRISERMTKENLVT
                     APEGTTLEEAEEILQRHRIEKLLIVDDKGRLKGLITVKDILKKKRFPNAAKDEFGRLR
                     VGAAVGVGSDYLLRAEKLVSEQVDVLVIDTAHGHSANVIKSVRELKRRFPQVDVIAGN
                     VATAAATKALIDAGADAVKVGIGPGAICTTRVIAGVGIPQLTAILDCAEVAMKSEIPI
                     IADGGIRYSGDIAKALAAGASTVMLGSLLAGLDESPGETVLYEGRVYKSYRGMGSIAA
                     MTRGSSDRYFQEGEETSKLVPEGIEGIVPYKGFLADTIHQLIGGLRSAMGYCGTATIN
                     ELQKRGCFIRVTQASIQESHPHNVKISKEAPNYRLEN"
     assembly_gap    23482..23581
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23842..24483
                     /locus_tag="PKX09_05965"
     CDS             23842..24483
                     /locus_tag="PKX09_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17403.1"
                     /translation="MMIILKILLVLFFILIFLLFVTLFSKTWLSIFGNFTEQTKTGKL
                     KVQLAGKLISIMVVMETPEVNFRIGILGLHFKVGQKKAPSLAKKKSPEPKPEIPKKPR
                     TRRYKFSIFDWIEFGKALTLRFFKLVHIETLSANLTVGLGNPAYTGLLMGAYYTIRET
                     VKDFQSVHIVPDFVAKKFTGKVEFRALIRLIKTIPILIFIVNFLRKMKNKKRR"
     gene            24487..24858
                     /locus_tag="PKX09_05970"
     CDS             24487..24858
                     /locus_tag="PKX09_05970"
                     /inference="COORDINATES: protein motif:HMM:NF021119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HOU17404.1"
                     /translation="MLEGLVDTLLSRLREIVASETVIGKPIETGDTMVIPITKISLGF
                     MVGSSGNPEEQKKKTEGTGLGGGALIEPLAVITIQKGEVKIHRLKEKISGVDKLMEIV
                     PEVLEKYFKPKCEKKSANENT"
     gene            24953..25630
                     /locus_tag="PKX09_05975"
     CDS             24953..25630
                     /locus_tag="PKX09_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOU17405.1"
                     /translation="MEIDILKNIPLFADLKDDIIQQIYNLMQKRAYKKGNIILMEEEF
                     GDTLFILSKGSVKITRMGEDGREVILSILGEGDFFGEMSILDGENRSANVVALEDTAV
                     LILKRGDFLDLLEKHPQIAIALLQEMATRLRRSDQQIEGLSLSDAENRIAMCLLRLAE
                     DLGVIKQGKVIIENLPYQQDIANMAGTSRETVSRMLTLLQRKGYIQKYGRRLIINNYD
                     GFVKQFS"
     gene            25902..26675
                     /gene="tatC"
                     /locus_tag="PKX09_05980"
     CDS             25902..26675
                     /gene="tatC"
                     /locus_tag="PKX09_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOU17406.1"
                     /translation="MARINSTNAEKEMGFLDHLEELRWRIIKILIALVLGTVGAFLFS
                     DQAIDILMRPARQVGSEMTIQVLKIQGLLMLKFKVAFLLGLVIALPIVAYQFWAFISP
                     GLLQNEKRWGPGLIIGVTVFFLLGAIFAFFVLIPIALKFLIGIGVAGVERNISIEYYT
                     KFVMQLILATGLIFQMPVLSFILTKIGLITPQFLRKSWRYAIIILMVIAAIITPPDPV
                     SMIFMTLPLLFLYEISIWISRIASRPKIQNETDQIELAG"
     gene            26682..27662
                     /locus_tag="PKX09_05985"
     CDS             26682..27662
                     /locus_tag="PKX09_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOU17407.1"
                     /translation="MPPKDLNNIFAPISTELDLFQKEFRQALYSEVELINQIGSYILS
                     GSGKYIRPALVLLSANLCGKSNEHSIIAASVVEILHTATLVHDDIIDDSEFRRGRPSV
                     KAIWSNKIAVLIGDFLFSRALSNMLRLQDFTVLELLSQTSELLSSGELLQLEKSLNNN
                     MDEEIYYQVIWAKTASLFATACQAGALTVAANEEQTKALFDYGRCLGLAFQIEDDLFD
                     CTGTSIHTGKPVARDIKQNLVTLPLIHALKTLAPEERHRIRQQLKNRPSPEQITAITE
                     LIINTNGITYAENKCRELIQQAVAALAIFPDSQYKSALINLANYTLMRNN"
     gene            27659..28702
                     /locus_tag="PKX09_05990"
     CDS             27659..28702
                     /locus_tag="PKX09_05990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOU17408.1"
                     /translation="MTTTTNPNSILIMRLSSMGDIVVLTPIFRSLREKYPLARIDFLT
                     KMEFAEIVANNPFLSNIITYDSGTKLAGWRDLCRQLKAQDYDLLIDMHNNIRSHILSH
                     YLRRVSLLRYQKPYLRRFLLFYFWLNFFPHDFRLLKEYYKVLQPLGINYPPTAKPEIF
                     LEPEAVTKAQDILVKYEISEPFIIILPIANWPNKLFPLEKYINVARRINTDLQMPVLW
                     LGGKADIHLQQLDYLVPGKSVRLAGETTLSESLALIGLSRAVIGNDTGLTYAAEALGK
                     PVILILGPTARETGAGCYAPKSIVLEAQLFCRPCSQKGDRRCYRRQRQLCFNQITDAQ
                     VYDALVKIIGAES"
     gene            28699..>29575
                     /locus_tag="PKX09_05995"
     CDS             28699..>29575
                     /locus_tag="PKX09_05995"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOU17409.1"
                     /translation="MTIFWLVIYNAIVMPIAYSGLMILSHFNSKIKEGLKGRQTLWLN
                     LQKFREELPKRTVYLVHSASLGEFEQAKPVIRGLKTLRPDILLVASFTSPSGFKNAER
                     MPEVDLFTYLPIDTFLQTRRFLQKLRPTKIIFVTYELWPNLIFHANRYKIKTYLISAR
                     IRPDSSKLKPIIRHFFADLYRSLDYIYAISEEHKQSVLKLIGSVKITVLNLGDTRYDQ
                     VIERSKLLANKMVPRVFNDGFVFIGGSIWPQDARPLLPALYQCLKNFPDFKIILTPHE
                     PNDYALEMLEDEFQTH"
BASE COUNT         8400 a         6262 c         6559 g         8254 t
ORIGIN      
        1 tttacaaatg gcttgaacaa gtcaattggt acggggaaaa tcgtcgttga attattttca
       61 gcggcaactt cgcgtaaagt ttgcagaaag cgtaattgga ttgtaatagg ctctttgttc
      121 ataatttctg cggcttgggc aagtttggtg gcagcctgaa attcgccttc ggcattgatg
      181 actttggcgc ggcgttcccg ttcggcttca gcctgtttag ccattgctct ttgcatttct
      241 tgaggtaggt caacgtgttt gatttcgact tgtgaaacct taatgcccca gggatcagta
      301 tgagtatcta aaattgtctg cagttccgcg ttgatttttt cacgactgga aagcagttca
      361 tcgagttccg cctgacctaa aatactacga agagttgtct gagataattg agaagtggca
      421 tataaataat cctcaacttc gacgacggct ttggagggat caacaacccg gaaatagacc
      481 accgcattga ctttgactga tacattgtct ttggtgataa tatcctgcgg tggtacgtcc
      541 ataaccactg ttcgcagact taccttgacc atccgatcaa ctaccgggat aaggaaaaag
      601 agccctggtc cctttgcggc aatcaagcgg cctaggcgga atatgacacc acgttcgtat
      661 tcacgcagaa cccgaattgc gctggaaatt agaaaaatca gcaggataat aatagttatt
      721 aatggaatgc tcatcttaat ctccttactt gttatttatt tgggtttaac tttcaaaacc
      781 attctatcaa catcaagcac aacaatttcc gttcctttgg gaatgggcgt atcggaacgg
      841 accttccagt attcgccatg aacgattgcc atgccggttt tatctacgtc ggtaattgtc
      901 tgaccagttt caccgatcat accttcaatt ccggtagtcg gtttgcgttt atgagccttg
      961 agcgccagcg taagagatat aatgacaaaa gcggccgtaa aaattacaat cggcagtatc
     1021 acttttaacg agacggcttc taaaatttca ggagcttgat ttacatcgat caacattaat
     1081 gaaccgagtg tcattgcaac aatccctcca atggttaaca atccgtagct aattattttt
     1141 acttcaagta taaagagtat aataccgaga ataatcaacg ccagaccagc atagttgatc
     1201 ggtagaacct ggaaagcgaa aaatgctaaa ataagaaaaa tggcacccag cactccagga
     1261 aaaattgcac caggattaga caattcaaaa taaattccgt aaaagcccag cattagtaga
     1321 atataagcca cagtaggatc actgattaca tccagaatcc gatggtggat gccaaccgga
     1381 cggaatcgga tagtcgcctc ttgagtgtgg acagtttttt caccggaagg aagtttgaaa
     1441 gttttcccat cgagaatttc taatagttca tctaagtcat ctactacaac atccacgacc
     1501 tgaagtttaa cggcttcggt ctcggtaatg gaagcgctct cgcgcacggc tttttccagc
     1561 caatcttcat tacgaccacg ttcgcgggca agacttcgga tataagcgac cgcatcatta
     1621 gtggcttttt caatcatagt ctgggaacta gcagaatctg gctggccgcc gattcctccg
     1681 ccacccagat tgaccggatg agccgcgcca atattcgttc cgggcgacat cgccacaaaa
     1741 tgggctgaaa cggcaataaa tactcccgcg gaagcacact gcgccccgcg cggactaacg
     1801 taaacaacaa ctggaatttc tgagttaaga atattcttga ttatatcccg catagaagtc
     1861 attagcccgc cgggagtgtc catctgaatt atcagacact cgacctgatc gggctgagct
     1921 ttgcgcaaat tgtcgttgat ataggaagct gaaaccggat tgataacacc gtcaacttcc
     1981 agcacccaaa tttctttagg ggtcgcggtc agtaaaatac tgccaaccag caagatcaag
     2041 aaaattttcg gtaaataaaa gcgactcctc ataattatat tgaatataat ccgtataggc
     2101 gtgaatctca aatctttttt taaaagtcgg tgagattaaa ttttcttagt gctgactatt
     2161 tggaccctga tgggcttttc agccggtcgt tcgggaggga aagccagcgc agtaaaatct
     2221 tcaaactccg cgatgataaa gtaatctaaa taattaccac tgaccatttc cgggattaga
     2281 ggcagaaacc agatgccatc ccgatattgc atattaaatt ctttatataa ctcagaagaa
     2341 tcatcgcgca ggaaaatttt aacccatttt aattggctgt cgcccggcgt aatcacacag
     2401 gtaatttctg ccggattatt ttgaatcaaa gtttttggtg gaatatgata gatatgtggg
     2461 atttcacccg cgatggaaaa ttgcatcaat agaacgaggt aaaatattaa agatgaaatc
     2521 actctcaccg taatatcaat cttaaagggc ttttaatctt tttcagtgtt tttagccgat
     2581 actatcgtga taaaaattaa tttagaatag cacagccagc gagattgagc agcgattttc
     2641 ggtgtaggct tgggttgtcg tcgtttcaaa atagcgatag aaattagaca gattgccgaa
     2701 tccatgctga aaactacagc cgatgatgat ggattctttc agaattgttt cgatacccgc
     2761 ccccataacg tggtaatatt cctcatcagg ctcatattcc gcataaggcg atggcactaa
     2821 gcggtaagct aggcgcgtcg aaacaaaatt gccgaatgga atggtcagaa ccgcaccgat
     2881 atcagtcgta ttgcgcagat tccagtagag atcgcggttg acctccctca tttcgtccaa
     2941 taaatccact tcgatctttt gccagttatg gaaaatgaaa tccataccca gcgaaatttt
     3001 accaaccttt atggcaaaat tgggagcgat ctcaagagga taacgcgttc tgtatttgaa
     3061 atcgtaagaa tttggaaaga tagaatccac agtatcataa gaataagtct ccgtcagttc
     3121 gctcacctca tgcactttaa gtttcagagg agtcgaaagg cgtaagccga ttttataatt
     3181 ttccgattga tagcacattc cgaagtccat attgaatcca gaataacttg gtttgatatt
     3241 ttcttggtaa ataatagaat taaaatctaa ttcgtcattg acgtcaacac ctatttcagt
     3301 tgactcataa ttacgctggc cgctgtagaa gttaaatgac aaaccaaggt taaaattttt
     3361 cataaattca gtggcgccgg caaatcggaa tacatttaga gtcccggact cattaatatc
     3421 atattccagg tcagtatcaa cgatgtgagc atttccgtcc tcgtcgtaat aagccgcgcc
     3481 ggtagttcct ttggtatatg aggaagaata ataataagcc gccggtgaat aggaagccgc
     3541 cattacaaaa ctaccttgat aaaccggaaa aggataagca aatcctatgt aggaaacatt
     3601 atatttagtg gtggaagccg aatagtcttt tcctccatcg gctaaagtag tcaaattctt
     3661 gggagtaata ctgattccag aaaaactgcc tttaggcctt ttaacattta tcagaaaagc
     3721 agggttagac tgagtcgaac cgatatccgt ttccaaatat gaattgttca ataatgtaaa
     3781 tgaggaagct cctataccat aaatactcca gaatggtcga agggcttcgc tactcattac
     3841 ttgatcgaaa tgctgagcgg aaattgacat tggtaaaaaa aacaccaaca tcgccaaaat
     3901 aaaaaaatac cggcagaatt tggacatatt aactcctatt tgcgattgtt tttcgatgaa
     3961 tcccccgaag ctttaggcga agatgaacgg ctactaccag agctggaaga agaacgactg
     4021 ccggatgaag atgaactact ggatgaagac gagctgctgg acgatgagga gctactggaa
     4081 gagctggctg gttgataaga tgaaccgcta gagcttgatg aactcgaaga ggaattgcct
     4141 gtatttgagg aaccggagct ggaggaagtt ccttttctaa cggtggcacg aggcgaactg
     4201 gatttctgcg atgcggttga tctagttgaa ccgcgggaat tggtgtttgt ggtaatcact
     4261 gtggttttag gagccgaagt tgggactaac gtggtctgaa agccgacgac ggctttttcc
     4321 tgggaactat ttcccgctcc tgtcgtcttg ggcgaataac gagctttggt cgagccggaa
     4381 ttggtggtag tcaccgtagt tttttcgcct gatacggacg tggtcgttat ggcactggtg
     4441 gaagtaggca gataagctgc gccgctttga gaactggcgt tatttgaggc gccgcgactg
     4501 ccgctgggag ttgatttcgg gacataatcg gcttttaact catcacgtcc ttcgttgtag
     4561 taggttccat cccgatatcc atgccaatga tgcctatgat aggggtatcc gccatagtaa
     4621 tagccatagt agccgtaatg agggtaatat ccataatagg gataataacc gccgtagaag
     4681 gaaaagtaac cataaaagcc ccagtcataa aacggatcgt aaaaccacgg atcataatat
     4741 ccgagatacg gtgaatgata gcgatagcca ccataaaatg gcgagtaaat attgaaacct
     4801 aaccagagtt gactattcat tgagtcccaa taaccatcac ggtaaccaag acgataatca
     4861 tttaaataag aagagtcaaa tgtgtaattc tcataagttt cttgttcctg gtaagttgta
     4921 tcggtaactg attgttgata ggtttcttgg cggacgatcg gtgcggaaaa ctgagtataa
     4981 caactggaga taatgagggc ggccgtcatt aaaataattt gagttaatgc gttcgctttc
     5041 atattcattc ctttattttt agttttcatg gcatttaatt agaccgtgag atctccaaat
     5101 ttaatccgga tagcaatgat aataagcagt aaatttgaca attagttttc attataggtt
     5161 aaattgcact cgctctaata taaaaaagtt ccttcaacta gttcaactag cttatatatt
     5221 tttctccgga tttttaccat tgatgatcaa gctggctggt aatatttgaa aaccatattt
     5281 ggtttcgatg gtaccccaga acacaatact gcgattttgc aattttggta aagcttttat
     5341 gggaacaatc atttgattcc attgattagg ctggcaatct ttgaaaatta attcgcgatc
     5401 gtaactcact tccctgcttt ctgcagaaca taggagttgg agccgtacgc tgtcgagctt
     5461 ttcggcgtac agaggtttca aatcaatttg taaagcggct tcttccccag gcattaaaaa
     5521 gccgtctcca ttggaatcaa gaaaagacaa actggcggca aaaccactag cttttttatc
     5581 cacaggctgg gattgataga aaatatcatc ccctttcttg gaaggcaaaa cgtcaacgta
     5641 aaaagtctgt gaatcaatgc catcataatt atcctgaaca gctaccgtga actcaaaccg
     5701 accggaatcg atcggttgcc agaccaaaat accggtattt ttcaggattg tggcattttc
     5761 cggaccggac accacagaat aggttatttt atcgaacaga tcaatttcaa cggcttgtac
     5821 tcgatagaaa taaagttcgc cttgccgagc ggtttcgact gggacgctcg taattaccgg
     5881 cggattattg atattagtcg gcacaaaacg gggcgatcct tgaatatttg gttcagaata
     5941 gggaaaaatt gtccaatttg aaaccgcggg cgattttagt aattcagcct cctcgatgtt
     6001 ctcccaacgc aggttagata tgacaaggtc acccttgaat attccgcgat aggtaaaagg
     6061 attttcccga gaaaattgcg gcacggtatt gccaacgtaa acgacattcc ggccggttgg
     6121 tgaccagtcc accgaaaagg ctaagtgttt tacatttttg actaatgtat caggttggat
     6181 ggaaattgat gcaaaagccg tgtcatataa tttaaacccg aaaggttcac cgtttggacg
     6241 gctggcgatt gcccattcgc cacttggtga aatcgtccag ctcagaagcg gctcggcaaa
     6301 tatttgcaat attttggtct ttagcgcatc gaaatcagtc tggttgacac gaacctgctc
     6361 gggttttcga ttctcaagcc agataaagcc cacccgatct tgggtcatca tcggaaatat
     6421 attaatcccg gaatggcttt ttgtcagaac acgatgtcgg ttttctctta aatcatacaa
     6481 acgaatatta ccatccaatc cacccgtgta aagtatggta gagccatcag ggagaattgt
     6541 gggataattg ccataaatcg gaagcggcgt aacatttttc gtaacaatat ccagcaggta
     6601 gatttggcgg gcaagcccct gactaaaaat tacctggcgt tgatcggctg aatatgaagg
     6661 gtaaagtata tcgtcagtgg tgctataaat cttttggggc aaaccaccca caattggaat
     6721 ctgataaatt ccgtcccaga gatcgccatc tattcgccca ttgaaaatta gatctaatcc
     6781 atcaggggaa aagcgtggca cgatcaggtt attggcaatc aggtttttaa agagcacttt
     6841 aggactctga aaaatgaaaa ccggctcgtc atttgccttt tggctttcgc aggtcaaaaa
     6901 ggtcaacgcc aataaactta agataaagag ttttactgtt ttcatattct catcttgttg
     6961 gagcgcatac tattctattc acctcagaag agtttaaaca gtaaaataat gggattcaat
     7021 aaaaatattc accattaaaa aataataaat tccaatactt aaaataccgt cccgaatttg
     7081 gattgaacag ttaaatgaat ttatactttg agaaattcaa ggtatcaacc tgtgataaaa
     7141 aggtaacttc ctgttcttac tttgagtaaa tctttaaaaa gttgaaattg cggccaattc
     7201 ggtcgccagc tgttcgtcca ccatacaaat tattttttta ttaaaaaatg agaccggtta
     7261 tgtatattat cctcgtgaaa cgttcatttt ttaagaaaat aggccgatta tgttggtttt
     7321 cgaccggttg gattggagca cggtatattt atcgtgaact aatctttccc tttttatttt
     7381 ctatcggagt tgtgactttt attttattgg ccaatttttt aattaaatcg gttgaccgac
     7441 tgctgggaaa aggtttgcca ctcggcgtca tttttgaatt tctttatctg aacttagcct
     7501 ggatagtagc gatgtcagtt ccgatggcgg ttttagtagc gaccttaatg gcttatggtc
     7561 gtctggcgga ggataatgaa atcaccgcca tgcgttccag cggtataagc tttacgacga
     7621 ttctcgtacc gggatttatt tttggtctgg tggtatcagt agtgatgctg tatttccatg
     7681 atggtgttct gccagaattt aatcaccgag cgcgtctcct caccagtgat atttaccgca
     7741 aaaggcctga tctgaacatt gaaccagggt acttcgttga cgatctaccc gattattcca
     7801 tttacgttaa agaaaaagtc ggcgagagtc tgcgcaaagt cactatttat aataaggatg
     7861 cccgagacat tcagaccacg atctatgccg atagcggata cattggagta gaaggcaaca
     7921 caattctatt tacccttttc tccggtcaaa tccacgaaat tgatttgaaa ggactgtcgg
     7981 attaccgtcg aattgatttt gagcgtcatc gaattgctat tccggttgat aatttaacgc
     8041 tcgaacgacg cgaaagtgag cggcgcggcg atcgagagat gacgatcaaa atgatgcgcg
     8101 ccgaggttga gcgttattcc agagaatatc aacaagtcat tgccaaaatt aacaatttag
     8161 tccgcaagga gctgggtgat accctgactt ctttcgattt gaaaaagatc gagaaaaaag
     8221 ttcaaactct tcaggcggaa aatctgaaga atctaccgcc cgccgaagcc gcccgccgca
     8281 atcagaagtt gactattttt ctaaaccgca ttcgaagtga aagcaatctg gctttcaatt
     8341 ataaaaaaca gattgccaaa tataatgtcg aaattcaaaa gaaatattcg attccgtttg
     8401 cctgcattgt ttttgtcctg ctgggtgcgc cgctgggcgt tattacccgg cggggtggca
     8461 taaccgtctc cgccgttttg agtattttct tctttttatt gtactatgtg ttcctgatcg
     8521 gaggcgaaga attggctgat gcggctatta tttcaccttt ctgggcgatg tggacgccga
     8581 acttgatcct attaatcgtt ggtatatatc tgatctatta tacttccagt gagcaacgcg
     8641 tgatagattt ttcacggttc ccgtatcttt ttcgccgttt gaataaaaaa attgaaaaaa
     8701 atccataaat aagcctggag aaaagtaatg ccgtctgtca aacaaatgaa acgaaccaaa
     8761 aattttatta tggtggatct gatagaaaag aaaaaagctg gattggaaca tagcaaagag
     8821 gaaattcaat atttgatcgc gtcctatacc aaaggtaaaa tacccgatta ccagatggcg
     8881 gcttggctga tggcggttaa ttttcaagga atgaccgacg ccgagacttt tgcgttagtg
     8941 gaaacgatgt tactttccgg tacgaaactc gatttgagtc atattaaaca ttgcaaggtg
     9001 gacaaacaca gtaccggagg tgtgggagac aaagtaagct tgattctggc gcctctggca
     9061 gtcgccgcgg ggtgcgttgt gccaatgatt tccggacgcg gactcgggca tactggtggt
     9121 actttggata agttagaaag cattccggga ttcaatgtaa atctgagcag tcaggaattt
     9181 gtccgcttag tcgagaaata tggtctggcg atgggcggcc aaaccgagga actcgtcccc
     9241 gccgaccgta aaatctatgc tcttagagat gtaactgcga cggtcaattc cattccactc
     9301 atttgtggca gtattttaag caagaaaatc gctgaaggta ttgacggctt ggtgctggat
     9361 gtcaaaaccg gtaacggcgc tttcttagcc gactacaacc agagtcagca actagctaaa
     9421 cagctgaaac gggttgggga aaatttcggt ttgcaaatca aagcggttat caccgatatg
     9481 aaccagccgt tagggcgagc agtcggcaat tggctggagg ttgtcgaatc aatcgtaacg
     9541 atcaaaggtc agggtccgga agatttaacc gcaattacgc tcgatctaac tgcttggatg
     9601 gtcgttctgt ccggaataga aaagaatttc ggcgaggctc gcgaactact aaaacagctg
     9661 attatttcgg gcgaggctta tcaaaaattc ttactaatgg tagaagcaca gggtggctat
     9721 atcagctaca tcgaacatcc ggagaaatat aagcaagccg cctggcaggc gtcggttctg
     9781 gcgaccgaat cgggttgggt gaaaaacatt gacacgaaac ggattggtat gtgcgctgtg
     9841 gcattgggag ccggtcggca atcgctggac tccgtcatca aaccagaagt tggattgatc
     9901 attaataaaa aaattggcga ttatgttgaa gttggggaac cgcttgtaac cgttcatgcc
     9961 gacagtcgca gggctttcca gaatgttcag agcgccatcc gtaaagccta tcatttttcc
    10021 aaggcaccgg tgacgccgcc acctttattt tatgaaactt tttaatttag attttagaaa
    10081 agcggttaat tatttctttt tttaattaaa tttacccttg ttaactaaac cagttagtca
    10141 ggttttgtta gaaagtcgaa attttaatga attatccaaa atctaaacgc tcgtccggaa
    10201 ttttaattgc cgccgaaggg ctggacggtt ccggtaaatc aactcagctc tatttagtta
    10261 aacgctggct cgaactcagt ggtcaccatg tttatttgac ggaatggagc tcgtcggaga
    10321 tggttcggca ggcaatcaag cgcggtaaga aaaagaacct cttgacaccg accacattta
    10381 gcttagtaca cgccgtagat tttgccgacc gctatcatcg ccaaattcaa ccgcttctga
    10441 aaactggctt cattactcta tctgaccgtt atattttcac tgcgtttgcg cgtgatgcgg
    10501 tgcgtggatg ttccagcgcc tggttgcgca acctctatag ttttgcgaaa atacccgaca
    10561 ttacttttta ctttaaattg tctccccaaa ttgcggctga gcgcattctc tccaaccgcc
    10621 caaagattaa atattatgaa gctggtatgg atttgaatct gcatcccgaa gtctatgata
    10681 gcttccgaat ttaccaaggc aaaatcggta ccgaatacga ccagatgagt aaagaatttg
    10741 ggttcgtcga gatcgatgct actcaaccga ttaagaccca acaggaaaaa attcgggcaa
    10801 ttattgccga gaaaattgat ctggcgtcgt ataaaaaggg ggaactggaa tgaaaagctt
    10861 ttacctcgaa ccgctcccaa aatgtgacat cagtagactc agtggtaaat taatcgtctt
    10921 ggaaggttcg gacggctcag gtcgcacgac tcagattgct cgcatcaaat cctggctgga
    10981 aggcaaaggc tatgcggtcg tgaactgcga actggctcgc tcgcaattgg ttggtccgga
    11041 actggagttg gctcgccgcg gcaataccct ctgccccaca accctttctc tattttatgc
    11101 taccgatttt atggatcagc tcgaaaatgt catcataccg gcgttacgcg cgggtttcat
    11161 tgttctcgcc gaccgttact tttatacctt aatggcgcgc gatctggtgc ggggcactga
    11221 ccttaaatgg gtccagaata tttacggtgt ggctttagtt cctgacttgg ttttttacct
    11281 gaatgtctct ccacgccgtc taatcgagcg ccgctggcag aaagccaacg caatggacta
    11341 ttgggagtcc gggatggata tgggcttatc gcgcgactgg gcggtaagtt tcttgattta
    11401 ccagcagtac ttacggaagg tttttctcaa gttgcaagat atgtatggtt ttatcgttat
    11461 caatggcaat aaatcaatca cagccatcac taatgatatc gtgaagcaaa tcgaaaaagt
    11521 tttaatctaa gagatcttct accagaaatt ttatttgaca attccataag atatttttat
    11581 atttaatcta cttatgagat ttgttatgaa ttattttgag ctatttcatc gtctccatca
    11641 tcattccaga ataggaaccg ggatgagata agctatttac agttattgtt aatcaacccg
    11701 gtcccttctg taagtgcacc gggtttttta ttttcaggag caattatgat tactttagct
    11761 attcaaaaaa caggacggtt aaacgaacac accaccaaat tgctgagcga atgtggactc
    11821 ttcgttggca atggtagtac ggcacggcta atgaccgaag cgtctaattt tccaatgcga
    11881 attcttttcc tgcgcgatga cgatataccg gaatgtgttg ctgacggcct ggctgactta
    11941 ggaatcgttg gggaaaatgt ctatcatgaa aaagcgcgtc cgttgaaaat tgtcgagcga
    12001 ttgggttttg ccaagtgccg ccttgcgatc gccgtaccga aagattttaa gtacgaaaag
    12061 ctgacggatt tgaatgggct gagcatcgcc acttcatatc ctaacattct gaggcgattc
    12121 ctgaacgaaa ataaccttaa agccgagatt catgaaatca gtgggtcggt cgaaatcgcg
    12181 cccggtatcg gactagcttc cgccattttc gacatcgtca gcaccggtag tactctgctg
    12241 accaacgggc tgaaagaagt cgtgacagtt ttaaaaagtg aagccgtact ggtagcctcc
    12301 gaaaatttaa gtccggaaaa aaccgatttg ctggaacaat tacttctcag aatccgggct
    12361 gtccaagccg cgcatactcg caaatatatt ctcctcaatg ccccagattc cgccatcgaa
    12421 aaaatctgcg ccattttacc gggaatgaag agccctacga ttatgcccct ggctgagcct
    12481 ggttggagtt cgctccattc ggtgatcgag gaacatcagt tctgggaacg tatttccgcc
    12541 ttaaaagcgg ctggtgcaca gggaattctg gttattccaa tcgaaaaaat gataatgtag
    12601 gatgccatta tgaaagttat aaaatatcct caaagaactt cctggccaga gttaattcgc
    12661 cgaccagcga ttgataataa aatcttacga aatttagtga ttgaaattat agaagcggtt
    12721 aaaaccgacg gcgatgacgc cctccggcaa ttttcctgga aatacgataa tgtgcgtttg
    12781 aaagaaatcg agatcctgcg cgatgaattg gcatctgtcc cgaatgaagt agatgatgac
    12841 ctccggaaag ccatcgagat tgcgcgtgct aatattacca aatttcattc ctctcagttt
    12901 gaaggaattg aaaaggttga gactactcct ggcgttatct gctggcgtaa aagtgtgccg
    12961 atcgaaagtg tcggattgta tattcccggt ggatcggcgc cgctcttttc gacggtgctg
    13021 atgctaggta ttccggcgcg gctggctggt tgtcccgaaa ttattttgtg cacaccgccc
    13081 aataaagacg gtcgcattca tccggtgatt ctttacacag cccaaatttt gggcatcgag
    13141 cgaattttca agatcggcgg agctcaggca atcgcggcga tggcttacgg aactcagagc
    13201 gtaccgcgtg tcgccaaaat cttcggacca ggcaatcaat acgtgacaat cgccaagcaa
    13261 atcgtcagcg ctcagggcgt agcgattgat atgccagctg ggccttcgga agtggcggtc
    13321 gtagccgacc ggttcgctaa tccggaattc gtggcggctg atttactctc acaagccgaa
    13381 catggcgccg atagtcaggt catttgtgta accgataacg aagaattaat tccgcaagtt
    13441 atcacggcgg tcaagcaaca gctggcggaa ttgccgcgcc gcactatcat cgaaaaatca
    13501 ctggaaagta gtcgcctgat tttggtcaat aatctggatg aagcattaga attcatcaat
    13561 tgttatgcac cggaacatct tattcttcaa acccaaaatc ccgagcagtg ggcggataaa
    13621 gtcattaacg ccggttcggt ttttctgggt gattttactc ccgaatcagc cggcgattat
    13681 gcttccggta ctaatcatac cttgcctact aacggttacg ctcgcaatta tagcggaatt
    13741 tcggtggaca gctttgtcaa gaaaatcact tttcagcaga tttcgcctgc cgggctaaaa
    13801 gccatcgctc cggctattga ttgtatggct accactgaaa agttgcaggc gcatcgcaac
    13861 gcagtccgaa ttcgcctgga agcgattgaa aaaggagctg gacgtggttg atctggaaaa
    13921 actggcgcgg cgcaatatca ttaacctcca gccttatgca accgcccgcg acgattatag
    13981 cggcgcgatc ggcatatttc tcgacgccaa tgaaaattct ctcggtagcg taatcccaca
    14041 aaaattcaat cgctatcccg atccgcatca aaaacgccta aaacaggaaa ttgccgccct
    14101 taaaaatgtt aacccggaac aagtttttct gggcaacggc agtgacgaag tcattgatct
    14161 tttgatccgc gttttctgtc aaccgggaac agaaaaaatt atgatcctac cgccaaccta
    14221 tggtatgtat gccgtcagcg ccgccagtaa tgatgtcggt gttgtttccg ttgctttaac
    14281 tccgaacttt cagattaatc taccgcaaat ttttgagcaa attgcgggcg tcaagctgat
    14341 ttttctctgt tcgccgaaca atccgactgg taattgcctt aatccagcgg atgttgtcaa
    14401 tttgctggag aaattcaagg gaatcgtcat aattgacgaa gcctacatag acttcgcacc
    14461 ccaaaaaagc ttgctgccaa gactgaagga gttcaataat ctggtaatta tgcaaacttt
    14521 ttcgaaagcc tggggcttgg cgaatctccg actcgggatg gctttcgccg atcctcagat
    14581 tattgattgg ctgtcgcgcc tcaaatatcc ctataatgtc aacggcttga cccaggagct
    14641 ggcgttgtcg gctttagaac gatctgcgga aaaggatcgg atggtcgccg aactttgcac
    14701 cgagcgggaa cggctagcag gttcacttga ggaattaccg atagttgaaa aagtctatcc
    14761 ttctgacgct aacttcctgc tggtgaagtt tagggatgcg cgcagtgtat ttcaatattt
    14821 aattaatcag aaaatcattg tacgcgaccg ctcgcaagcc ctacattgtg aaaattgcct
    14881 gcgaattacc atcggtactc ctaacgaaaa tgatatttta attgccaaac tcaaggattt
    14941 tgcacgttga agaaagtact ttttattgac cgcgacggga cattgattat cgaaccaccg
    15001 gatgaacagg tggattcact ggagaaactc gaattccttc cgggcgccat cacaaatatg
    15061 aacaaaatcg ccacccaatt tgattaccaa ctggtaatgg ttaccaacca ggatggactc
    15121 ggaacggatt tatttccaga aaaagatttc tggccagctc acaataaaat gttacaaatt
    15181 ctggccggcg aaggcgtagt attcgctgag attttgattg atcgtagcct tccaaatgac
    15241 aatgccccat cgcgcaaacc tggcatcggc atgttaacag attaccttaa gggtgattac
    15301 gatctcgaaa attcatttgt aattggcgac cgggagactg acgtacagct ggcacgaaat
    15361 ctcggcacaa aggcgattta ccttggaagc aacgaatcta tttcagccga ttataaggct
    15421 aaaaattggt cggaaattta taattattta agcaatatta gtcgcacagc caccgtcacc
    15481 cgcaaaacta acgaaactga catcagcctg gtaattaatc tgaatggcaa aggtgcaaat
    15541 cgaatagaca ccggtattgg ttttcttgat catatgctga atctattcgc tacgcattcc
    15601 ggttgcgatc ttgatttgac tgccagaggc gatctcgcag ttgatgaaca tcatacggtc
    15661 gaggacgttg cccttgttct cggcgaaggt ttgctaaaag ccctcggtga taagaaagga
    15721 atcgaacgtt acggttttct tctgccgatg gacgagtcac tggcgcaggt ggcactggat
    15781 ttttccggac gacgtgaatt cgtctgggaa tgtgacttca aaagggaata tatcggaaaa
    15841 atgcccactg aattattccg tcactttttc aagtcctttg ccgattcggc cgcctgcact
    15901 ctgcatatta aagccaccgg tgaaaatgaa catcataaaa tcgaagcagt tttcaaagcc
    15961 gtcggtcggg cagtgaaaat ggcaattcgt cgcgatccaa cctgcgacca gattccgagt
    16021 agcaaaggca aattatgaaa gtggtcataa ttggctataa ttccggaaac attcactcgg
    16081 taaggctggc acttcaacgt ctaaatgtca aagccgttgt cagttccgac ccggccgtga
    16141 ttcaagccgc ggataaagtc attttccccg gggttggtga agcctcttcc gcaatggaat
    16201 ctatcaaggc gaatggtatt ttcgagatat tgccacggtt gcagaaaccg accctgggta
    16261 tttgtctcgg tctgcagttg atgtgccggg attcagaaga gaatgctact caatgttttg
    16321 ggattttcga cattgctgta agaaaatttc caccgctcgc caaagtgcct caggttggtt
    16381 ggaatacact tttcgattta aaaggtgatc tttttcgcga tgttgctgaa aattcctacg
    16441 tttactttgt tcatagttat tatgccgaat tgagtgaaca taccgttgcg cagaccgagt
    16501 atatcattcc ctattcagcc gctatgcaga aggacaattt cttcgcagtg cagtttcatc
    16561 cagagaaaag cggtaaagtt ggtgagcaaa tcctgcgaaa tttcattaaa ctatgattac
    16621 aatcataccc gcaattgata ttataaatgg caagtgcgtg cgccttagcc gcggcgattt
    16681 caatactcaa aaaatttata acgagaaccc attggaaatt gctcgacaat tcgccgatgc
    16741 cggtatcaag cgtttacatc tggtagatct ggacggcgcg cgccagaagc gagtagttaa
    16801 ctgggaagta ctggaaaaga ttgcgacggg gacgcctttg caaattgatt tcggtggcgg
    16861 gattcggagc gagacggatg tgcggattgc tttcgagagc ggcgcacggc aaattactgc
    16921 cggaagcatc gcaatctccg cacctgattt agtggaagaa tggttgaaaa aattcggcgg
    16981 cgaaaaaatc attctcggcg ccgacgttat cgatcgaaaa atagccatcc acggttggca
    17041 agaaaaagcc ccactggaaa taatggaatt tttacagaat tttcaggaaa aaggcatcca
    17101 acaaacgatt tgcaccgaag tcgcacgcga tggaatgttg actggaccag cttttgaatt
    17161 atatcgagaa atcaaacaaa tatttcccga tctttacttg attgccagcg gtgggattag
    17221 ccgaattgac gatatttatc agttacaaga tgatggcatt gacggcgtta tcatcggcaa
    17281 agcgctttat gaaggtcgcc taaaattgag tgaattgaaa ggatttttat gttagctaaa
    17341 cgcattattc cctgcctcga cgtcaaagat ggacaaacag tcaaaggcgt aaattttatt
    17401 aatattcggc gtgccggaga tccggtagaa ctcggcgcgg cttatgccga gcaaggcgct
    17461 gacgaattgg tttttcttga tatcacggct tcaatcgaaa aacgcggaac atttatcgaa
    17521 ttggtgcgcg aaattgcgaa acatttgaac attccgttta ccgttggcgg cggagtacgc
    17581 aacgccgatg acgtcgctcg cctgctggac gccggcgccg acaaaatttc aatcaatacc
    17641 gcggcggtca atcacccgga aatgatcgaa aaattagccg ggcgcttcgg cagtcaatgt
    17701 atcgtaatcg cgattgatac caaacatacc gaaaacggcg acttcgtata tattaatggc
    17761 ggaaggaccc caactaacca aaccacgcaa gcctgggcac ggcaagtcgt cgagctcggc
    17821 gctggtgaaa ttctactaac ttcgatggat cacgatggca ccaaaagggg ctttgcgctg
    17881 gaaattacgc gtgaacttgc ggaaaattta ccgattccgg taatcgcgtc tggcggggcg
    17941 ggcacaatgg aacatttttg ggaagtgttt aaaataggaa aagccgatgc ggcgctagcg
    18001 gctagtgtct ttcacttcgg cgaaatcccc attccggaat taaaaaaatt ccttaaatcc
    18061 aatggtatcg aggtgcgatt atgaacattg attttactaa aaataatggc ctgttaccgg
    18121 caattattca ggatgaaaag actggaaaag ttttaatgct gggttatatg aatcgagaat
    18181 cttatgaaaa aacaaaggcg accggcaggg ttactttttt tagtcggagt cggcaggcac
    18241 tctggacgaa aggcgaaacg tccggaaatt ttttagaagt caaggagatt ctaagcgatt
    18301 gcgacggcga cacgcttttg atcaaagtta aaccaaccgg accaacttgc cataccggcg
    18361 ccgatacctg tttcaatgaa aaaaacatag ccgagaagcc gttcattttc gaactggagg
    18421 agattattcg cgatcgccgc aataatccga aaccaggctc ttacactaat aagctattcg
    18481 atgccggaga cgaacgcatc gctcagaaag ttggcgaaga agccaccgaa gtcgtcattg
    18541 atgccgtgac cggtaatgaa atgcgcctgc cagaggaagt tgccgactta ctatatcatt
    18601 tgcttgtgct attagtagcg aaagatgtcg cgctcgacga tattatgaat gtgctggaaa
    18661 aacggcatag aaaataaaca attaaccttc agaagttttc caatggatac aacccgacta
    18721 gccgtttact ttggctttac tactaaagcg acagtcactc gaaatagcat ttttctattt
    18781 attcagacat cctgatttct ttccagaaaa gtggattcta cattcctgca gataaatact
    18841 aaatctcatt ctaattatag ctaattaatt ctaaaaattc cgcttcgttg agaattttga
    18901 cgcccagttg ccgggcggtt tcggctttcg agccggcatt ttcgccgacg acaacgtagt
    18961 cggttttttt actgacggag gaggcagatt tgccaccgta tttttctacg agtgcttggg
    19021 cttcttcgcg ggtaaaattg gccagtgtgc cggtgaaaac gaaagtcttc ccggcaattt
    19081 ctcctttttg tttgtaatcc ggttcggtac taatacgaat tccactgctt agtaatttct
    19141 caattgtttg acggttggat tccgcattga aaaaattgcg gacgctccgg gcgacgattg
    19201 gtccaacgcc ttcgattgcc attaattctt cttcagaagc ctgactgagg cgttcgaggc
    19261 taccaaatgc ttttgccagc actttggcca tatgttcgcc aacgttccga atgccgagtg
    19321 catagatgaa gcgcgccaga ctaacatttt ggctggcggc gatggcatcc agtaaatttt
    19381 gggcagattt ttcgcccatc cgttccaggg tcaataattg ttcttttcgc agagaataaa
    19441 tgtcggcaac attttttatt aaaccgctat caaccaattg ttcaatcaat ttctcgccaa
    19501 ggccttcaat attcatagcc cgtttgctag caaaatgttc gatactggct ttgatctgag
    19561 ccggacaatt aatatttgga caacggtgtt tagcttcatc ctcctcgcgt acaaccggcg
    19621 ttccgcagac cgggcaattg gctggaatcg aataacgctt ttcgttgccg gtccgtcgct
    19681 cagtgatgac tttaaccact tgcggaatga catcgcctgc ccgctgaact acgacccaat
    19741 caccaatgcg aatatccttg cggtcaattt catcctgatt atgaagagtt gcatgggata
    19801 cggttacgcc gccaatttgc accggcttta aatgggctac cggcgtaatg gcacccgtcc
    19861 gtccgacgct ggcttcgatg tcaattatct gagttacttc ctgctgagct ttaaattttc
    19921 cggcaatagc ccagcgagga ctgcgtgatt ttattccgag tttagcctgc tgagctagag
    19981 aatcaacttt atatacgaca ccatcaatgt cataaggcat cgagtcgcgg atggattcaa
    20041 ttttatgata atacgctata agttcttcga attccatagc tatttcaatt aatggattga
    20101 cccgaaagcc ccatttgagt aaagcttcca gaaattcatg atgggtttga aaagaatagc
    20161 cttctaaggc tcctagtccg taacagaata ttttcaatgg gcgtttagcg gtgatctgtg
    20221 gatcgagttg ccttactgag ccagccgccg aattgcgggg attagcaaat agcggttttc
    20281 cttccttcaa ttgttgttcg ttcagacgct gaaaatcagc tttatccata taaacttcgc
    20341 cgcgcacttc cagtagagtc ggaatcggca aattcggcat taatctgagc ggaatactgc
    20401 ggatcgtttt tagattttgg gtaatatctt cgccggttat accgtctcca cgagtagaac
    20461 ccgaaatgaa aacaccattc tcataaacga gttcgactgc caaaccgtca atttttggtt
    20521 cgcatacgaa ctggacggtc tcacccggca aacttttttg cacccgaatt gcaaaatcgc
    20581 ggatttcgcc ctcgtccatt gcattgtcca gactgagcat aggaacgcga tgggtaacgg
    20641 ttttgaactc gtctaaaggt tgggcgccga cgcgttgagt gggagaatcc ggcgttctca
    20701 attccggata agccgcctct aactgttcta gttcacgcat taggcgatca tattcagcat
    20761 cgctaattgt gggattatca agaacgtagt aattgtaatt agcagtctct atttgacggc
    20821 ggagttcctt agccttttcc cgtagtgcat ccaaattcat aaataaattt actgcctcct
    20881 atttaaaaat ggtatgagaa ttcacaacga ttttttatca aactataatg gagtaaaaaa
    20941 taaattggga atgtcctaac gtattttggc aaatgccggt aaaaatgatt attgagttac
    21001 ggagagccgt ttgccggggt gttcagggga tcaatctttg ggaatgagtt ctccacaaac
    21061 cggacaaatt agataaacat tcggttcacc gaaatagcgt aaattggtga tgcgaaaatt
    21121 cacctgcccg gacatattcg gacgctgggt aataattata ctgcccgaag cagtattttg
    21181 tggtttaagc gggatatcgc tattcagcac aatcacaatt tcctgttttt caataacgtt
    21241 gccgtattta tcgtaaatat ctataaatcc agctaattcg ctaacggatt tcatcgaagt
    21301 attttttacc tcgaccctaa tttcaattaa gttattgtcc agatcggaaa ttattatatc
    21361 cgaaacctcg acggcttgct gggcaaatgc gattgtgcta aacatccaga tcagaaatat
    21421 ttttttcatg gattgcatcg cttacctctt ttattacaat gcaatataca ttaaatcaat
    21481 tttatagtca agttatcagt tagggagttt taaagtgatc ggcttcgggt aaaatattgt
    21541 ttaaatattt tcgtaaaatt aattttctaa cactatgaaa atttcttatt tttttgtagt
    21601 ttacttaatg gtaggcaaaa caattcaatt tctcaaaagc gccaagaata aaggccggaa
    21661 acaaaaatgc tcctgccagg ttgtttcatc ggcttttagt ccagaaaaag ctatttacgg
    21721 cgttgatacc gaattcgaca agcttagtcg aatgggaatt ataccggaag tcttacctat
    21781 taattattcc ccggttacga tacaccggaa gccattgtta tcgatatatt tataccaaaa
    21841 tatatgaggt tgtctatgaa tgaaaaaatc gcacgcgaag gcttaacctt cgatgatgtt
    21901 cttctaatac catctgaatc aaacgtactg ccgaaagagg tcagtctcga cgccaagctg
    21961 accaaaaaaa ttcaattaaa cataccaata cttagtgccg ctatggatac tgtgacggaa
    22021 gggaaaatgg ctatcgccat tgctcgcgaa ggcgggatcg gtattcttca taaaaatatg
    22081 agtatcgaac aacaagtagc cgaagttgac aaggtgaaac gcagtgaaag cggtatgatt
    22141 gtcaaaccaa tcacactatc ctccgatagt acaattcggg atgctctaaa tctaatggac
    22201 cgttattcaa tttcggggat tccggtggta gataacggtc aattaaaagg tattctgacc
    22261 aaccgtgaca ttcgtttcga gaccaacctt gatctgcgca tttcggaaag aatgactaag
    22321 gagaatttag ttaccgcacc ggaaggcacg acactcgaag aagccgaaga aattcttcag
    22381 cgtcaccgca ttgagaagct cttaattgtg gacgataaag gacggcttaa aggattaata
    22441 acggttaaag atattctgaa gaagaaacgt tttccgaatg ccgccaagga tgaatttggg
    22501 cgtctgcggg tcggagccgc agttggtgtc ggttccgatt acctcttacg tgccgaaaaa
    22561 ttagtcagtg aacaagttga tgtgctggtg attgacactg cccacggaca ttctgccaat
    22621 gtcatcaaaa gtgtacgcga gttgaaacgc cgctttcccc aggtagacgt aatcgccggc
    22681 aatgtcgcga ctgcggccgc taccaaagcc ttaattgacg ccggagccga tgcagttaaa
    22741 gtcggcattg gtcccggtgc catttgcaca acgcgggtga tcgccggggt gggaattcca
    22801 caattgacag ccattttgga ttgcgccgaa gttgcgatga aaagtgagat tccgatcatt
    22861 gcggatggtg gaattcgtta ctcgggcgat attgccaaag ccttagcggc aggagccagt
    22921 accgttatgc ttggttcact tttagccggt ttggacgaaa gtccaggtga aacggtatta
    22981 tacgaaggtc gtgtttataa atcttaccgc ggtatggggt caattgcggc gatgactcgt
    23041 ggcagtagtg accgatattt tcaggaaggt gaagaaacca gcaagttagt tccggaaggc
    23101 attgaaggca ttgtaccata taaaggcttt ttagcggata caattcatca actaatcggc
    23161 ggtctgcgtt cagcaatggg ttattgcggc actgcaacaa taaatgaatt acagaaacgg
    23221 ggatgtttta ttcgtgtcac gcaggcatcc attcaggaaa gccatccaca taatgttaaa
    23281 atcagtaaag aagccccgaa ttaccgcctg gaaaattaaa gagggcaata attatactac
    23341 tgacgttcag ggcagattag ctaaatactc agccagtgcc gacttacggt gcgcggcatt
    23401 atttttatgg ataatacctt ttcgagcgac cttgtctatc agtgccactg cggctttgaa
    23461 aaggggttca gcttcagcct tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23581 ncgaccttgt ctatcagtgc cactgcggct ttgaaaaggg gttcagcttc agccttagtt
    23641 ttagtatttt taaccttttt aatcgaggtt ttcatcattg agacatagtg ccgatttctt
    23701 aaggcggctt tttgagcctg acgagttcgt tttaaaacag acgcattctt tttcattaaa
    23761 ctccttttac aaagattacc aaaaagtagg caaatttatt tatattgaag ctcattttca
    23821 aataaaaaac ctccgaaacg gatgatgatt attctaaaaa tacttctcgt cttatttttt
    23881 attttgattt tcctcctttt tgtaactcta ttttcgaaaa cctggctgag tatttttggg
    23941 aatttcaccg aacaaactaa aaccggcaag ctgaaagttc aattagccgg taaactgatt
    24001 agcattatgg ttgtgatgga aacgcccgag gtaaattttc gtattggtat cctcggctta
    24061 catttcaagg ttgggcaaaa aaaagccccc tcactagcca agaaaaaatc acccgaaccc
    24121 aaaccagaga ttcctaaaaa gccccgaacc aggcggtata aattctcaat atttgactgg
    24181 attgagtttg ggaaagcact gacactacga ttttttaaat tggtccatat tgagacatta
    24241 agcgcaaatc ttaccgtcgg tttgggaaat ccggcctata ccggtctttt aatgggcgct
    24301 tattacacga ttcgagaaac agtgaaagat ttccaatcgg tgcatatcgt cccagatttt
    24361 gtcgccaaaa aattcaccgg taaagtcgaa ttccgggcat tgattagatt gataaaaaca
    24421 attcctatac ttatttttat agtgaatttt ttgagaaaaa tgaaaaataa aaaacggagg
    24481 taaattatgc tggaaggttt agtagacacc ctgttaagtc gtttacgtga aattgtagct
    24541 tctgaaacag taattgggaa accaatcgaa actggagata cgatggtaat ccctattacg
    24601 aaaatttctc tcggttttat ggtgggcagt agcggtaatc cagaagaaca aaaaaagaag
    24661 actgagggca ccggcttagg aggcggcgct ttgatcgaac cgcttgcagt cattacaatc
    24721 cagaaaggtg aggtgaaaat tcaccggctg aaagagaaaa tatccggcgt ggacaagctt
    24781 atggaaatcg tcccggaggt gctggaaaag tattttaaac ccaaatgcga gaaaaaatca
    24841 gccaacgaaa atacgtgatt acttatttca tcgaaaattg ttgaaaagtt tttcttggtt
    24901 ttttgccgga gtgaacctat tttcttagcg ctttatatat gagagtagtg atatggaaat
    24961 agatatatta aaaaatattc cattatttgc cgatcttaag gatgatatta tccagcaaat
    25021 ctataacctt atgcaaaagc gggcatataa aaaaggcaac attatcctga tggaagagga
    25081 attcggtgat acgctcttta ttctcagtaa aggaagtgtc aaaattacta gaatgggtga
    25141 ggatggccgt gaggtaattc tttcgatact cggtgaaggt gattttttcg gtgaaatgtc
    25201 aattctggat ggcgaaaatc ggtcagctaa tgttgtcgcg ctggaagaca ccgcagtttt
    25261 aattctaaag cggggcgatt ttttggattt gctggaaaaa catccacaga tagccatcgc
    25321 tttactccag gaaatggcta cgcgtctgcg ccgtagtgat cagcaaattg aaggactttc
    25381 gctaagtgat gctgagaacc gaattgcaat gtgcctcctg cgcttagcgg aagatctggg
    25441 cgttatcaaa cagggaaagg tcataataga aaacctgccc tatcagcagg atattgccaa
    25501 tatggcgggg acttcccgcg aaactgtttc acgaatgttg actctactac aacgtaaagg
    25561 ttatatccag aaatatggtc gtcgtctgat tataaacaac tacgacggat tcgttaaaca
    25621 attcagctga agcacgacct ccttaagtac gtgctgacta cacaatttta ggttaattca
    25681 aagaaaaata ttttatttat aaaaataaca attagatact tcacccataa tttgataata
    25741 aataaataca aatctctcga accctgctaa ttttaccgat aaaatagaca ttctctatta
    25801 tatgaggatt agtaataatc tatgactttc acgtgtagga tatttatatt tacaccgcat
    25861 ttgaaaaaaa ttatgagtat ttattaagtt taccattaac aatggcgcga atcaattcta
    25921 ccaatgccga aaaagaaatg gggtttctgg atcacctcga agaactgcgt tggcggatca
    25981 tcaaaatttt gattgcacta gtattaggca ccgtaggggc tttcctattt tctgatcaag
    26041 caattgacat tttgatgcgt ccggcgcggc aagttggctc cgagatgact attcaggttc
    26101 tcaaaattca gggcttattg atgctgaaat tcaaggttgc ctttctactc ggtttggtaa
    26161 ttgctctacc gatagttgcc tatcaattct gggcgtttat ctcaccaggt cttctgcaaa
    26221 atgaaaaacg ttggggcccg ggattaataa ttggagtaac cgtattcttt ctattgggcg
    26281 caatttttgc cttttttgtt ctaattccga tagctctcaa atttctgatt gggattggtg
    26341 tagctggtgt tgaacgcaac atttcgatcg aatattacac gaaattcgtc atgcagttga
    26401 tactcgcaac tggattgatt ttccagatgc cagttttgtc tttcatcctt accaaaatcg
    26461 ggctaattac gccgcaattt ctgcgcaaat cgtggcgtta tgccatcatt attttaatgg
    26521 ttattgccgc tattattact ccacccgatc cagttagtat gatttttatg accctccctt
    26581 tattatttct atacgaaatc agcatctgga tttcgcgcat cgccagtcgc ccgaaaattc
    26641 aaaatgagac cgatcaaata gaattggctg gatagttcat aatgccgcct aaagatttaa
    26701 ataacatatt tgcaccaatt tccactgagc tcgatctgtt ccaaaaagaa ttcagacaag
    26761 ctttatattc cgaagtggaa ttaatcaacc agatcggcag ttatatatta tcaggaagcg
    26821 gcaaatatat tcggccggca ttggttctgc tgagcgccaa tctctgtggt aaatcgaacg
    26881 aacattcgat catcgccgcc agtgtcgtcg agattctgca taccgccact ctggttcatg
    26941 acgatattat agacgattcg gaattccgtc gcgggcggcc ttctgtcaaa gccatctggt
    27001 cgaataaaat tgctgtatta atcggagact tcctttttag ccgagcttta agcaatatgt
    27061 taaggttaca agattttaca gttctcgaat tactttctca aacttccgaa ttgctcagtt
    27121 ccggtgaatt attacagttg gaaaaaagcc tgaacaacaa tatggatgag gaaatatact
    27181 atcaagtgat ctgggctaag actgcttcac tttttgccac cgcttgtcag gcgggcgctc
    27241 tcacggttgc cgccaatgaa gaacagacca aggctctctt cgattatggt cgctgtctcg
    27301 gattggcatt ccagatcgaa gacgatcttt tcgattgcac cggcacatct attcataccg
    27361 gcaaaccggt agcgcgggat attaagcaaa acttagttac tctgccgtta atccacgctt
    27421 tgaaaacctt agcgcccgaa gaacgccacc gaattcgcca gcagttgaaa aatcgccctt
    27481 ctcccgaaca gataaccgca ataacggagc tcattatcaa taccaatggc attacttatg
    27541 ccgaaaataa atgccgggag ttgatccagc aagccgttgc ggcacttgct attttccccg
    27601 actctcaata taaatccgcc ctaattaatc tggctaacta cacgcttatg agaaataatt
    27661 gacaacgact actaatccca attctattct gatcatgcgc ttgagttcga tgggtgacat
    27721 cgttgttctc actccaattt tccgtagttt aagggagaaa tatcctttag cccgcattga
    27781 ttttctgacc aagatggaat tcgctgaaat agttgccaat aatccttttc tatcaaatat
    27841 catcacttac gattcgggga caaaattagc tggctggcga gatttatgcc gccaattgaa
    27901 agcccaagat tacgatttac tgattgatat gcacaataat atccgcagtc atattctttc
    27961 tcactatttg cggcgtgttt ctcttctgcg ttatcaaaaa ccttacctga gacgttttct
    28021 gcttttctac ttctggctga attttttccc gcatgatttc cgtcttttga aagaatacta
    28081 taaagtttta cagcctcttg ggattaacta tccgccgacc gctaagccgg aaatttttct
    28141 cgagccggaa gcagttacca aagcgcagga tattcttgtt aaatatgaaa tttccgagcc
    28201 gttcattatc attttgccca ttgcaaattg gccaaataaa ctatttcctc tcgaaaaata
    28261 tatcaatgtg gcgcgccgaa tcaacaccga tctacagatg ccggtactct ggcttggtgg
    28321 aaaagctgat attcacctgc aacagcttga ttatttagtt cctggtaaga gtgttcgttt
    28381 agcaggcgaa accaccttaa gcgaatctct ggcgctgatt ggtctcagcc gggcggtaat
    28441 cggtaatgat accggcttga cctatgcggc ggaggccctg ggaaaaccag tgatattaat
    28501 tctggggccg accgcgcggg aaaccggcgc cggctgttac gcaccaaaaa gtattgtcct
    28561 ggaagctcaa ctattttgtc gtccttgttc tcaaaaaggt gatcggcgtt gctatcgccg
    28621 ccaacggcaa ttatgtttta atcaaatcac cgatgcgcag gtttacgatg ccctggtaaa
    28681 aataatagga gccgagtctt gactatattc tggctggtaa tttataatgc tattgttatg
    28741 cccattgcct attctggatt gatgattcta tcacatttta attccaaaat taaagaagga
    28801 ctgaaaggac gtcagactct ctggctaaac ttacagaaat tccgggaaga attgcctaaa
    28861 cgtacagtct atttagtcca ctcggcatcc ttaggtgaat ttgagcaagc caaaccggtg
    28921 atccgcggct tgaaaaccct gcgtccggat attttgctgg tagccagttt cacctcgccc
    28981 agtggattca aaaatgccga acgaatgcca gaagttgatt tatttactta ccttccaatt
    29041 gatactttct tacaaacccg ccgcttttta cagaaattac gaccgactaa aattattttt
    29101 gtgacttacg aattgtggcc gaatctcatt ttccatgcta accgatataa aataaaaact
    29161 tatctgattt cagcacgaat tcgtccagat agttccaaac taaagccgat cattcggcat
    29221 tttttcgctg acctttaccg ttcgctggat tatatttatg ccatttcaga agaacataaa
    29281 caatcggtgc tgaagctaat cggctcagtc aagattaccg tattgaacct cggtgacact
    29341 cgctatgatc aagttatcga gcgctccaaa ttactcgcta ataaaatggt tccgcgagtt
    29401 tttaacgatg gatttgtttt cattggtggt agtatttggc ctcaggatgc ccgtccgctt
    29461 ctaccagctc tctaccaatg tctaaaaaat tttcctgatt ttaaaattat cctaacgccg
    29521 catgaaccca atgactatgc tttggaaatg ctggaggatg aatttcagac gcata
//
LOCUS       DAOSMP010000027        29220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_1606_length_29220_cov_15.370924, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000027 DAOSMP010000000
VERSION     DAOSMP010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 29220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29220
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1606_length_29220_cov_15.370924"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /gene="glmS"
                     /locus_tag="PKX09_06000"
     CDS             complement(<1..1133)
                     /gene="glmS"
                     /locus_tag="PKX09_06000"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOU17410.1"
                     /translation="MCGIVAYIGDKSAVPLLLEGLKKLEYRGYDSAGICFAGDHRLEV
                     VKRSGKIAELENTGVLDSISSHIGIAHTRWATHGAPNEINAHPHLSCHGEIAIVHNGI
                     IENFRSLRDMLIQEGHHFSSETDSEVLAHLIEKFYEGDLATAVVKALHNVEGTFGIAV
                     MHKDEEQLVIARRGSPIIIGLGNHETIVASDAVSIIPHTKKVIYLKDNEIAVIGFNDC
                     HLQNLDGQFIDSDVQEIRWNISQIEKRGFKHFMLKEIFEQPEAVQNTLRGRITNGQIK
                     LSLNTELERVRRIVIAACGTSWHSALIGKNLIEKYTGLPTEVDYASEFRYRESLIDST
                     VLMIVISQSGETADTLAALRKAKSACTQTLGIINVVGSTIAREV"
     gene            complement(1466..2008)
                     /locus_tag="PKX09_06005"
     CDS             complement(1466..2008)
                     /locus_tag="PKX09_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOU17411.1"
                     /translation="MFRLINQELSNPVFDWFMPFISSKSFWLIPIFIGLVLLFWLGKK
                     RGRIATLLIILTIATADPFCARVLKPTFKRLRPSHELSDVRLLGKKGGKYGFPSNHAA
                     NVAGSMLILAFFYRRYKYICGVLAFLVGYSRIYLGVHYPTDVLAGFVIGIGISVGWIL
                     IWRSLANYLLKKRNDLLALS"
     gene            2220..2570
                     /locus_tag="PKX09_06010"
     CDS             2220..2570
                     /locus_tag="PKX09_06010"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOU17412.1"
                     /translation="MSNLIMLKSKIHNARVTATNLEYEGSIEIDAELIRLANLHVFEK
                     VLVLNLNSGVRFETYVIEGKAGQRDISLNGAAARLVEKGDRIIILAFAVVSPAELTDW
                     HPTIIRLDANNNPL"
     gene            complement(2684..3862)
                     /locus_tag="PKX09_06015"
     CDS             complement(2684..3862)
                     /locus_tag="PKX09_06015"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOU17413.1"
                     /translation="MAGMQDILKNSQESNNQNNSQKGKNEKNDNNFVGLSEMEAVKKA
                     ITENLGSTTNTFNSAADSTPVDFEPDLVITPADFPKNGFFKDSHLDEKKLKEIQCQDE
                     ITANQLYLDLLHELGNVFQSLKDNKPLDPQPLEKFIDLMHNSITSGDFLLLKAIQHKR
                     YAAWLISHSANVTILAYKIGLDLKYDRPTLHKLGISALLHDIGMLKVPNNIIFKHGQL
                     TADEFEIIKKHPLDGFEMAKHLEQYPHIIEVILQEHEREDGSGYPQGLPGDEIAEFAK
                     IVGLADVFEAMVHGRAYREGFITYTAIQKIIEKRSKQFNPRIIRALINSVPMFPVGSL
                     VKLSTNEIARVLSVNSLRPVRPVVEILIDSDGNRLKNPIRLDLEAEPLIYIAKPLAES
                     "
     gene            complement(3868..4665)
                     /locus_tag="PKX09_06020"
     CDS             complement(3868..4665)
                     /locus_tag="PKX09_06020"
                     /inference="COORDINATES: protein motif:HMM:NF017437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU17414.1"
                     /translation="MYLDYWKIKEKPFENSPNPKFLFHSKQHDEALFRLLYAIQSSRG
                     AALLTGEYGCGKTLLMHTIISELSQGKFKIAYLTNPRWSAMELIEEILYQLEINSKSK
                     SRLEMLHSLNDFLFQNVQEKCQTIIIVDEAQVISDYETFEELRLLLNFQLNDRFLLTL
                     FLVGQPELNNMIKNIPQLNQRIAVRFHLKHFNFEETKAYIQYRLKVAGRNDQIFTDSA
                     FDEIYKYSEGIPRIINNICDLSLVIGFGKHLSTIDHNVIKGIINSER"
     gene            complement(4690..5859)
                     /locus_tag="PKX09_06025"
     CDS             complement(4690..5859)
                     /locus_tag="PKX09_06025"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOU17415.1"
                     /translation="MNNHNKKILFRMLVLPFLMALLLPYVAKTWMFSHNLSKIYYLLV
                     TSLMTFGLIPIFYKLGVIMDITDKPGGRHIHQHATPRTGGIAIFITFIITLNIVTKPP
                     REFTGLFLASSVVAFVGIIDDIKHVPALIKLGAQILATIILIKYGIYLSFLPNHIIGN
                     IFSYVLTFIWIIGITNAYNCLDGLDGLAAGTAIIILLFYIIIATYMVADEFMAIVGLI
                     LLGAIIGFMPYNFRITKNALIFLGDAGSTFIGFTLAALSIYGNWGISKSVDLAIPILL
                     LGVPIADMIMTTITRIKDGKVKSVRQWLEYGGNDHFHHRLLQMGFTKTGSVLIIWAIT
                     TVLGLLSLLLKQGDRPEAIIAILIALILFALIISLMLFEYRQERRSLSNKKIFLK"
     gene            complement(5856..6899)
                     /locus_tag="PKX09_06030"
     CDS             complement(5856..6899)
                     /locus_tag="PKX09_06030"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOU17416.1"
                     /translation="MIPHYRPTFGQEEITAVTSVIRSGQLAQNGQVNLLEQILAENLD
                     RKYACAVSSGTMALILSLKALGIGPGDEVIIPSYTCAALWQAVTTVGATAIYADIEDE
                     TYNLAPPSVKLKMTPRTKAIIFPHMFGQPGHIQEVIEYGIPVIEDIAQAYGATIDKQP
                     AGHFGDIGVLSFYATKVIGAGEGGAILTDSPAILEKVRDLREYDEKDDLQPRFNAKMT
                     DLGAAIAIEQIKKYQSYFTKRTVIIQQYQKVDGIEFVLPLKNSRLKSNNYRCVAAHPH
                     KSAAECIALAAKLGACFRKPVYKPLHSYAQTESLPETEQAWQKQFSIPVFPAISNEDR
                     DRVTEILNRIAEL"
     gene            complement(6896..7534)
                     /locus_tag="PKX09_06035"
     CDS             complement(6896..7534)
                     /locus_tag="PKX09_06035"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOU17417.1"
                     /translation="MSSSEKKMNIIAIGSHPDDIEIGCGASLAKFNAKGHSVYLYVAT
                     LGGAGGDPEVRRAEQENSARIMGVKDIFWGSFEDTDITINKNSIAALEEIIHKVNPDM
                     ILVNYHDDTHQDHRNIAAITNSATRYIPNVLFYETPTTQNFMPNVFVDIGTEYLAIKL
                     RALLAHTSQINRTNIANLSIIQVAESSAHFRGVQARVACAEAFHSLRLFIDI"
     gene            complement(7531..8229)
                     /locus_tag="PKX09_06040"
     CDS             complement(7531..8229)
                     /locus_tag="PKX09_06040"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOU17418.1"
                     /translation="MICAIHQPQTYPWLGYFAKIMQADIFIFLDNVQFKKNEWQNRNK
                     IRTPQGWIWLTVPVIHHFGQKIDEVKINNTVNWRKKHLQTLQTYYGRAPFFKLYFEKL
                     ENLYQFEWQNLAQFNITAIRLLMSLIGIDTPTVIASEIKALNQNSQISAAERLIIATK
                     AVGANCYLSGIGGCNYLQPELFAKNDIKLKFQSFEHPVYRQMTNDFLPNMSVLDLLFN
                     EGQRANSIIQGGIR"
     gene            8297..8482
                     /locus_tag="PKX09_06045"
     CDS             8297..8482
                     /locus_tag="PKX09_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17419.1"
                     /translation="MDTIIKGCIIVGVISLILGIVSRLTLKPFFVEAQAFMQFAQFCF
                     LAAITFLLYKIAYKVKD"
     gene            complement(8494..10071)
                     /locus_tag="PKX09_06050"
     CDS             complement(8494..10071)
                     /locus_tag="PKX09_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17420.1"
                     /translation="MLIKRVTRICLNKKFAPFLITVSLLIMTDSLVFSQTKPGYILLK
                     TGVHFDSQVSGGKYSLAELANIIARHKLDVGIITDHDNMKVSYGIRPFENLLKFTIEE
                     NSVHRYGVEKYINEINALDAAYPNLILIPGIEAVPYYRWEGSPLYENMVLRDWHTHLL
                     VFGLEDPKVFQQLPSLANHNIGYSKPDGKIIKYIGENFTHFALMTLYFLLFLISLALI
                     FRRKYGLFSHKNINRPRQNIRFSFPAFIMAIIFGLILYNQYPFLPRKYTPYDRKAGSA
                     PYQELIDYVNAHNGLVFWAHPEVTHNEVRPVNIPLMNQAIKISTEAYPHRIMETQDYT
                     GFAIFWEGAKVIGRPGGLWDIALAEYCAGLRRKPVWAIGELDFEDSSNLNNVTETLTF
                     LFVKERSRAGVYEAMRAGRMYATRSFTGNKIVIDDFSVYDLHTGNNAFIGETLTNARP
                     PVAIHIRLRALTTLNKPETIFLYRNSELIKIFHLNTVLDEWFVDESPMPNPMYYYRIY
                     GGDSWLTLASNPIFVSK"
     gene            complement(10034..11155)
                     /locus_tag="PKX09_06055"
     CDS             complement(10034..11155)
                     /locus_tag="PKX09_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU17421.1"
                     /translation="MTRLDQGGSSNVTLKLCAGLVKNGYEVLLVAGRTTHPPFDLAEY
                     ARLNGFQLYFLRTLVRDPSPIYDPLAFLQIYRLILKYRPEILHTNTSKAGFLGRLAGR
                     LARVPKILHSTHGHIFYGYYNRYISKCFITLEKFSAHYCDRVLNLTEIGRQDHIRERI
                     GPSSKFYVTSGGADLDPFFKACHDNSTLKSPDEKIRICWVGRIEPVKNLPLLLRAAGI
                     LEHSGLNLEYIIVGDGADRQYNEKLVADLGLKNIQFLGYRTDVPEIMASSDIFVFTSL
                     NEGFGNVIIEAMACGLPIIGTRVGGVPSLIQDGVNGLLVNSDDAEALATAIQRIASNP
                     EQRQSMASTNRKKAEYFTIDNYIKRVIYAYQTCNTNLPE"
     gene            complement(11190..12392)
                     /locus_tag="PKX09_06060"
     CDS             complement(11190..12392)
                     /locus_tag="PKX09_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOU17422.1"
                     /translation="MSILHIPTAGTPYNLTEIARATLGLFESDHVRNNFSQTLKKYFD
                     VENLALVNSGTTALFILLELFKKIRTSPKRNEIILPAYTAPVLLLPIRAAGLNAVLVD
                     VDLTTFNMDVRKILNAVTPQTLAIMPVHMFGLPMDVAPIIESSAGQDIFIVEDAASSL
                     GSTIGGRQTGSIAPFGFYSLNRGKNVSTLAGGIIVWKDQRYSPIIEELVAGLPPVSQI
                     AEFLMWLNFLGLSIAVNPLCYTLLLPFIAKFKYTTLHPHFDSFAYTNMQAALGKTLWR
                     RIDQLTKRRVENGKALMQIFTGKPGYTIPKILPNSQAAFNQFPVIVPNLDQRQRLQKK
                     LLKVGIETTRLYEKPLHHFFPELNPFTYDNYPQATYLAERLLLIPTHAQIGPAILQKI
                     NSVVEALN"
     gene            complement(12394..13020)
                     /locus_tag="PKX09_06065"
     CDS             complement(12394..13020)
                     /locus_tag="PKX09_06065"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU17423.1"
                     /translation="MTTLNLKNQTRTAAFQTLTSIRVRTAALKIARQINGHFLDVGCG
                     NGLFLLEYYVTDAKKAIAFGLDHDRQAIYEARTLFKDNNISIEPLLIGDGFNLPFEDE
                     KFEVVFCLNTFLNFHPFEKIEAFIIEMHRICKKGGWIVFDYRNSRNPYLWFRYRLSSL
                     TGRLNVHGHRRKEFQPLMRRLNIKNSEQMAIPSPLPGFPLGYLNKWEK"
     gene            complement(13017..14174)
                     /locus_tag="PKX09_06070"
     CDS             complement(13017..14174)
                     /locus_tag="PKX09_06070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU17424.1"
                     /translation="MRNQNTLLIPGDFPPVVSGISTYYYQIWRLLPQENNYILCPKDP
                     GWEQFDAQTGLQTIRRRIPAGNLIRDKLLKGLLYAFWTLVLDRKYHFRKIHCGQVLSS
                     GFTGWLMNKLFGIPYNIYVYGSETFRFGYSPLFMKYIKTFLSNAEYIVPNSQFTCNEF
                     MALGIPPEKFKIITPGVDTGFFKPAPKDATLMEKYGLNGKTVLLTVARLDERKGHEVM
                     IRAVADLSSQFPDLIYLIVGAGREEERLRKLTKELNVTDQVIFCGYIPDAELPKFYNL
                     CDIFILLNRQTYDDVRLRGDYEGFGIVFLEAAACGKPVVAGDFGGVASAIENGRSGLI
                     IDANDQSAIRQTLVSLIQNPTLREQLGQYGLERARQSFDWQIISREIMKIL"
     gene            complement(14167..15099)
                     /locus_tag="PKX09_06075"
     CDS             complement(14167..15099)
                     /locus_tag="PKX09_06075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU17425.1"
                     /translation="MIISKPYTGNISRPFVKYPKGASEGRIEIHRPENVTEPLISIII
                     PTMGAARQEFLPGLMSQIERQTFRNIEVILIIGDTRQGRAINCGAALAAGRYLVIFDD
                     DTRLGSDDLLAKMLAQMENHPDIGLAGVANLIPENANWFVRRVMRELPRRTSPIVSEI
                     TDSDLAEHPCCIIPRDVFIKIGGENELIPRGLDPYLRQEIRQAGYRVVVLPELYIHHL
                     PPPTFSKFVRQFYRNGKMAAYVNKFYPEFVVELAYQHGAEVTQKRSTIVRIGSYVWRL
                     CKTIVTFRAFYLLSLVFYLSGYLIGYITLGEQDA"
     gene            complement(15142..17091)
                     /locus_tag="PKX09_06080"
     CDS             complement(15142..17091)
                     /locus_tag="PKX09_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOU17426.1"
                     /translation="MNKPNRFLCLLLLTSIPIWAETQPTFSDYPISAVDIKNVEINDA
                     FWLPKIKIIQDTTIQYAFKKCEEEGRMENFLIAGGRKKGPVRGKMPFDDTDVYKIIEG
                     ASYSLISRPNPALAAYLDSIISIIKIGQEPDGYLTTWFTIDRMHPPAWWAQPSPNRWD
                     HEESNHELYNSGHLFEAAAAHYRATGKQNFLKIALKNADLLVASFGPDKLRVPPGHQI
                     VETGLIKLYHITNNPQYVELARFFLELRGDSTSHKLYGEYSQDHLPVTKQTEAVGHAV
                     RAMYMYAGMTDIAAIYQDSAYFEALMKIWENIVTKKMYLTGGVGARHDGEAFGDNYEL
                     PNLTAYNETCAAIGSVYWNQRLFRLTGDAQYYDIIERTLYNGLISGISLDGKKFFYPN
                     PLESDGKYQFNQGACTRQPWFDCSCCPTNIIRFLPSVPELIYATIADSVYINLYISSN
                     ADIIVNQKKIEIIQQTDYPWDGKINIIVNPEKKTIFTLKLRVPGWARNEVVPGDLYKY
                     IAENPEKVSLLVNGKDENFVLDKGYIEITRKWTRGDKIELTLPLAVRKVVAHEKVTDN
                     VNKVAFECGPIVYCAEEIDNHRISEITVPLDTKLNITKGAVLTENVNILRGKVDNQEL
                     ILIPYYVWSNRGVGKMKVWLPINKE"
     gene            complement(17218..18690)
                     /locus_tag="PKX09_06085"
     CDS             complement(17218..18690)
                     /locus_tag="PKX09_06085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU17427.1"
                     /translation="MLKTGLAALRLMVFSVLTVSIAGAQNLGLTEFPIAVGEESAFCG
                     GGASGSTNGLIGILGDAQSRYNITAQLISQTGISGSRISLGQATTAPFPAGLPLVAFD
                     GTNYLVVWMEEITSTNYEVYGQFIDTSGNLVETAFLIATTNAKEEDNLRAPAIAFGGN
                     TYLVTWVNNGALIGRLIDKSGNLVGTEISINSSARENAVAFDGTSYLVAWVNEIPDTD
                     RTISAQLVSQQGALVGGNFIVDDSSYPSDNPISIGFDGSRYLIVFHEEMENEEWDLYG
                     RFVTTAGTAAERIAIADETGDQQFPAIAFDGTNYLITWFDGSAGSSKGRFFNTSGTPL
                     DLEFTIFGPLENKFPLLTGVSFASDRFCAITTRVDEDFTDGDVYGAFISPSTGIDESN
                     SHPAPATFELIGNYPNPFNASTSIKYTLPTSAQVEIAIYDIQGQLVRTLSKGEKSSGT
                     HIAIWDGKDNIGKDVSSGVYFCQLKTPTGILEIRKMLLLR"
     gene            complement(18988..20196)
                     /locus_tag="PKX09_06090"
     CDS             complement(18988..20196)
                     /locus_tag="PKX09_06090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU17428.1"
                     /translation="MAKLIDYRGIVGDKVITGIFQKARGLYGKKIIHLNSTYMGGGVA
                     EILNSLVPLMNDVGLDTGWRIIRGNPDLFTITKKFHNALQGDPINLSDMKKEIYIKAS
                     EDFSVFTHLDHDCVIIHDPQPLPLIRYYVKQQPWIWRCHVDISHPNPVLWDFLKAFIL
                     KYDLAIVSHETYKKDDIYIEQKVIPPVIDPLSPKNKELSEKDTKRFLKKFGIPIDKPL
                     LTQISRFDKWKDPEGVVRIFKKVKKQIDCRLVLCGSMATDDPEGMKIYQKVVDYSNHL
                     IQSGDIIPITSENNILVNSLQRYSDVIIQKSIREGFGLVVAEALWKKAAVVASNVGGI
                     PLQVIDGETGFLCEPNDEEAFAERIIQLMKDKKLKEKLGQNGKEHIRSNFLITRLLCD
                     YLDVIRSLLS"
     gene            complement(20284..22494)
                     /locus_tag="PKX09_06095"
     CDS             complement(20284..22494)
                     /locus_tag="PKX09_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HOU17429.1"
                     /translation="MRLLIVSNRLPITIIEKEGRLRFQDSTGGLVSGLSAYLDSLKGS
                     SFTRANHIWVGWPGTVVEDKSKKKLSTKLQDDFDAYPVFLAEKTMEKFYHGFCNKTIW
                     PLFHYFTSYVEYDEECWEHYQRVNEAFRDAVMEIIKPDDIIWIHDYHLMLLPKILRLK
                     ISNPIGFFLHIPFPTFEIFRLLPGTWRREILQGLLGADLLGFHSYDYTQYFLRCVLRI
                     LGYEHNMGQIIINDRIIKAETFPMGIDFQKFYNAVNSPEVQKEKNEFQKTLGNFKIIL
                     SIDRLDYTKGILNRLRGYEAFLDKNPQWHRKVILVLIAVPSRIGIEHYQEMKRQMDEL
                     IGKINGRFGSINWTPILYQFKFVPFNQLVALYNISDVALITPLRDGMNLIAKEYISAK
                     ADSAGVLILSEMAGAAKELGEAIIINPNNLPEITDALKIALEMPKEEQLKRNQVMQKR
                     LKRYDVVKWADDFIQDLLSLKENQKNFNAKLLSSAIRNRLIKDFSQANRRLIILDYDG
                     TLVPFVDQPQMASPDEQLLEILQSLAENPKNEIVLISGRDKDTLQNWFGKLDIGLVAE
                     HGVWLKEKNKDWDIIKPLTNDWKSKIIPLLEVYTDRLPGSFIEEKEFSVAWHYRMAEP
                     ELGSLRAKELTDYLLNFIANIDIQVQQGKKVVEITNAGINKGAAGLQWIGKKDFDFIL
                     AIGDDWTDENLFEVLPERAYSIKVGMVETHARFSLYNHTEVIELLKNLLKVNGN"
     gene            complement(22629..23468)
                     /locus_tag="PKX09_06100"
     CDS             complement(22629..23468)
                     /locus_tag="PKX09_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOU17430.1"
                     /translation="MPSDFGIIYREVSFKTDDGLVLKGWLYPPQDTTGLSKDFIGRMM
                     AMPDSLKPERKEYHYSNSHNPTIIICDGDAGNMAFSIFYAYHYFTNGYTVFTFDWRGF
                     GESAPWKIDQDQLCCAEFLTDYNAAINFIKDQPEVDSAKIGLMGFSTGAYLSFAMIAS
                     RDDISAYVGRALITSLNDLTTNLQKVSPNRNFVIPQNYPENLLPLKAANKVRTPVFLI
                     VGEKDDRTPVWMSKKVYKRLKGPKELWIVPEAEHGGAKGPEMITYPEFFTKTLSFYNK
                     YLK"
     gene            complement(23776..24519)
                     /locus_tag="PKX09_06105"
     CDS             complement(23776..24519)
                     /locus_tag="PKX09_06105"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HOU17431.1"
                     /translation="MRKPSLSIKIYIGLIITLAILTAINVFLPQGVFLPTQELPAPKS
                     ALALINAAIMLILYGGLGFLGLKLSQKLGYANLWDSKISNKQRFLIPALIGVSIGVFF
                     ILVDVILNQFHSLGSLPHPPFPTSLVASVVAGIGEELIFRLFFISFWVWLISYVLLKK
                     RWQNQIFWIIAIFSALAFAFGHIPSVMILFGLNTISEIPLALMGEIILLNGVVSLFAA
                     YYFRKFGFLAAVGIHFWTDVVWHIVWGVI"
     gene            complement(24779..26314)
                     /locus_tag="PKX09_06110"
     CDS             complement(24779..26314)
                     /locus_tag="PKX09_06110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU17432.1"
                     /translation="MKIKKLISNMFFKRYKPEYIVIAFFVIIKLMLQIMALSNSGFFS
                     DEFLHIEAGKHLAIGYADFPPMIAILAAIQNLFQSDSVFINHLFNLIASAMIIVFCGL
                     TTIRLGGKWLAVLTTLLCILFAPGMEGTQLLFLPTAFEQLFWVLCIYCIINYTQFTDS
                     KYLILFFIFGALGFLTKYSIAFLFVGVIISAVIYQRDIFSKKSLWVGLLIFFILIAVN
                     LYWQIINQFPVIHHFSQLYDRQLNQLSMGKELKKLVLFSNPLTTVFWLTGLLIIPFSS
                     KYKQYRIISFALLCSFILLFIAKGKFYYFFPVVLGLIPLGAVFYEQLLHNRKWIIFSY
                     LIVLGLVGTYLLPQGIPIIKLDRYIKLYNLKSNKDGKIPMDFDNYYSTEIWNQILGVV
                     KKTYTDLSLEEKRKCFILGRHYSMAGAVNLLGEKYGLPQAFSLHSSFEWMPEFDKDAV
                     IIAIGESNWLKQHWEEYFNDVREIAIVENKYANKVNKYNYRIFLCKKLKYSSTELKKL
                     IKG"
     gene            complement(27085..27846)
                     /locus_tag="PKX09_06115"
     CDS             complement(27085..27846)
                     /locus_tag="PKX09_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOU17433.1"
                     /translation="MKKITAFIGSARKKGTYLAVQEFEKNLKKYRDIDFEYVFLNDYN
                     LEFCSGCKLCFDKGEQFCPYNDDRNALIAKLEESDGVVFASPSYAFQVSGRMKNFIDR
                     IAFIYHRPRFFKKTFTAIGTQFIPFGKQTQKYLESVGDNLGFDVLKGTTVITPEPIPE
                     KQLEKLKANIKNLSDQFYKRLNREKAVKPSLFKIMTFRMTRSGLQSSDLKLYDYEYYK
                     EKGWFKSDYYYDIHLGPIQKAIGGLSDFLGRKLFK"
     gene            complement(28012..28785)
                     /locus_tag="PKX09_06120"
     CDS             complement(28012..28785)
                     /locus_tag="PKX09_06120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU17434.1"
                     /translation="MENEKPTIHEFDFNLISEYFIELDRQGPGSPESTIRALSFIDNS
                     SNELKIADLACGTGGQTLILAQNTKGTITGLDIFPDFIGKFNTNAEKLGLQNRVKGIV
                     GSMDNLPFQNEEFDVIWSEGAIANIGFEKGLKYWKGFLKKNGYIAVTYESWFTDERPA
                     EIEKWWFDAVPEISTIGQNILIMQKAGYVPVATFILPENCWIDNYFIPQKPRQEEFMK
                     KYAGNRTVEDFIGFMRREAELYSKYKQYYGYVFYIGKKI"
BASE COUNT         8679 a         5803 c         5910 g         8828 t
ORIGIN      
        1 acttcgcgcg cgatagtaga accgactacg ttgataatgc caagtgtctg cgtacaagct
       61 gatttggctt ttcgtaaagc cgctaaagta tcggcagttt cacccgattg agaaattacg
      121 atcatcaaaa ctgtgctatc aattagtgac tcacggtagc gaaactccga cgcataatct
      181 acttcagtcg gtaagccggt atatttttcg attaaatttt taccgattag agccgaatgc
      241 cagctagtac cacaagccgc aataacgatc cgtcgcacgc gttcaagttc agtgtttaga
      301 gataatttga tttgtccgtt cgtgatacga ccacgcaaag tgttctgaac ggcttccggt
      361 tgctcaaata tttccttcag catgaaatgt ttaaagccgc gtttctcgat ctgagaaata
      421 ttccagcgaa tttcttgaac atcgctatca atgaattgac cgtcgagatt ttgtaaatgg
      481 caatcgttaa atccgatcac cgcgatttca ttatctttta ggtaaatgac ttttttagta
      541 tgaggaatga tagaaacagc atcagacgct acaatcgttt cgtgattacc taaaccgata
      601 ataatcggtg aaccacggcg ggcaattacc aattgttcct cgtctttatg cattactgct
      661 attccgaaag taccttcgac attatgcaaa gctttaacga cggccgttgc caagtcgccc
      721 tcgtaaaatt tttcgattaa atgggctaat acttctgagt cagtttcaga cgagaaatga
      781 tgtccttctt ggattaacat atctcttaga ctacgaaaat tttcaattat accattgtgg
      841 actattgcta tttctccgtg gcaggacaga tggggatgag cgttgatttc attaggggcg
      901 ccatgagttg cccaacgggt atgggcgatt ccgatatggg aagagatcga gtcgagaacg
      961 ccagtatttt caagttcggc aattttgcca ctccttttaa caacttccag gcgatggtca
     1021 ccagcgaaac agattccggc gctatcgtac ccgcgatatt cgagtttctt gagtccttcc
     1081 aatagtagtg gaacagctga tttatcaccg atataagcaa caatgccaca cataaatgaa
     1141 tcctttctaa aaggtcataa aatcaagttg taaaatggat agaatcgagt taaggggtga
     1201 actaactagt tatggccagg gattcctcat ttggaataca attcgacttg gttggatttt
     1261 agaagttttt ataacccata aatcgtaatg cgaataaata tttttgttaa ccataaactg
     1321 tttttgcata aatataatag cagattttca cacagtgcta aaataggcaa tggagaagat
     1381 ttctgcaaaa aaaaggacaa ctttttgatg ataggaattc gaaaattcta aaatttttat
     1441 atactgctta tgccaattca aaatcctaac ttaaagccaa taaatcgttt ctttttttca
     1501 ataaatagtt ggctaaggag cgccaaatga gaatccaacc taccgatata ccaatcccga
     1561 tcacgaatcc cgccagcaca tccgtcggat aatgcactcc cagataaatt cggctatagc
     1621 cgacaagaaa tgccagcacg ccacagatgt atttatatcg gcgataaaaa aaagccagaa
     1681 ttagcattga accggcaaca ttggcggcat gattggatgg aaacccgtat ttgccgccct
     1741 ttttacccaa taagcggacg tcactgagtt cgtgcgaggg acgtaagcgt ttgaaggtcg
     1801 gttttaatac tcgggcgcag aacggatccg cggtcgcaat agttaaaata attaaaagtg
     1861 tggcgatccg tcctcttttt ttccccagcc agaaaaggag aactaatccg atgaaaattg
     1921 gaattaacca aaaagattta ctgctgataa agggcataaa ccaatcgaac acgggattgc
     1981 tcagttcctg attgatgaga cgaaacaatt taagatccaa ctggtaaatc aaactcggca
     2041 agtaaatcta aaccttcatt aaaaattcat taaacgtcaa ttaaaatggt aacaaaatta
     2101 gatattaaaa ccactcaaat ttatatttga acaaccaagt aagaaaacat gaaatttgaa
     2161 taaagtaact ttaattggta aattcgttat ggaaatgagt ttgattatag gaatgaatta
     2221 tgagtaattt gataatgctg aaatctaaaa tacacaatgc ccgagttact gccacgaact
     2281 tggaatatga gggtagtatc gaaattgatg cggaattgat tcgcttggca aatttgcatg
     2341 ttttcgaaaa agtgctggtt ttgaatttaa actcgggagt tcgcttcgaa acttacgtga
     2401 tcgagggcaa agccggtcag cgagacatta gtttgaatgg tgctgccgct cgcttggtcg
     2461 agaagggcga tcgcattatt atcctggctt ttgcggttgt atcaccagct gaattaaccg
     2521 attggcatcc cacgattatt aggctcgatg ccaataacaa tccgctttga tttctcatcg
     2581 aatctggcgt actgtacaaa aatccttttt aaaaaaataa aaaacgcctg cgcaaccgca
     2641 tagattgtgg ctgagtcagg caatttgaaa atgcggaaac cgctcaggat tcggcgagcg
     2701 gtttagctat ataaattaat ggctctgcct cgagatcgag ccgaatcgga ttttttaatc
     2761 tattgccgtc gctatcaatt agaatttcca ctaccggacg aaccggccgc aagctgttga
     2821 ccgaaagcac tctggctatt tcgttggtac taagtttgac caaactacca acgggaaaca
     2881 ttggtacact attgataagg gcgcgaataa ttctgggatt gaattgttta gaacgctttt
     2941 cgattatttt ctgaatagca gtgtaagtga tgaaaccttc gcggtaggcg cgtccatgaa
     3001 ccatcgcttc gaaaacatcc gccaaaccca cgattttagc aaattctgct atttcgtcgc
     3061 ctggtaagcc ttgcggataa ccggagccat cttcccgttc gtgttcctgt aaaatgacct
     3121 cgataatatg cggatattgt tcaagatgct tagccatttc gaatccgtcc agcggatgtt
     3181 tcttgatgat ctcaaattca tctgccgtta gctgaccatg tttgaagata atgttattag
     3241 gcaccttgag cattcctata tcgtgtaata gcgctgaaat tccgagctta tgtaatgttg
     3301 ggcggtcata ttttaaatct agtccaattt tatacgccag aattgtgaca ttggctgaat
     3361 gggaaataag ccaggcggca tagcgtttgt gttgaattgc tttgaggagc aagaaatcgc
     3421 cggatgtgat ggaattatgc atcaaatcga taaatttttc taacggttgg gggtcgagag
     3481 gtttattatc ctttagagat tggaaaacat ttcccaattc atgaagcaaa tccaggtaaa
     3541 gctgattggc tgtaatttca tcctggcatt ggatttcttt cagtttcttt tcatcgaggt
     3601 gactgtcttt gaagaaacca tttttaggga aatcagccgg agttatcacc aaatccggct
     3661 cgaaatcaac cggcgttgaa tcggctgctg aattaaatgt attcgttgtc gagccaaggt
     3721 tttcggtaat ggcttttttt acggcttcca tttcgctcag accaacgaaa ttattatcat
     3781 ttttttcgtt ttttcccttt tgggaattat tttggttatt tgattcctga ctatttttca
     3841 ggatgtcttg cattcctgcc atagatatta tctttctgaa ttaataattc ctttgatgac
     3901 attatggtca atagttgaga ggtgttttcc aaaaccgatc accaagctta agtcacatat
     3961 gttattgata attcgaggaa tgccctctga gtacttataa atttcatcaa atgctgaatc
     4021 ggtaaatatc tgatcattcc ggccagcaac tttaagacga tattgaatgt aggctttggt
     4081 ttcctcaaag ttaaagtgtt ttaaatgaaa acgaaccgct attcgctggt taagctgggg
     4141 tatattcttt atcatgttgt ttaattccgg ctgaccaacc agaaaaaggg tcagcagaaa
     4201 gcggtcatta agttggaagt tcaataataa tcgcaattct tcaaatgttt cataatcgct
     4261 gattacctgg gcttcatcta cgataatgat tgtttgacat ttttcttgga cattttggaa
     4321 cagaaagtca ttgagactat gcagcatttc gaggcgcgat ttggatttgg aattaatctc
     4381 cagctgataa agaatttcct caattaattc catagcactc cagcgcggat tggtcagata
     4441 agcaatttta aactttccct gcgagagttc ggagatgatc gtgtgcatta acaaagtttt
     4501 gccgcaacca tattcaccgg tcaggagtgc tgcccctctt gaactctgga tggcatataa
     4561 taaacgaaat agagcctcat cgtgttgttt ggaatgaaat aagaatttcg gattcggtga
     4621 gttttcgaaa ggcttttcct ttattttcca ataatccaga tacatatact taaatgtaag
     4681 ctaataaatt catttaagga atattttttt atttgataaa ctgcgacgtt cttggcgata
     4741 ttcaaatagc ataagtgaaa taattagggc gaataatatt aaggcaatca ggatagcaat
     4801 gatggcttct gggcgatcac cttgtttcaa gagtaacgat aacagtccca atacggtggt
     4861 aattgcccag attattagga cactaccagt tttggtgaat cccatctgta agagtcggtg
     4921 atgaaaatga tcattgccac cgtattcaag ccattggcgg acggatttga ctttgccatc
     4981 tttaatccga gtaatagtag tcattatcat atcggcaatc ggtacaccga gtaggagaat
     5041 tggaatcgcc agatcaacgc tcttactaat cccccaattt ccataaatac tcaaagccgc
     5101 taatgtaaat ccgataaagg tgcttccggc atctcctaag aaaattaagg cgttttttgt
     5161 aattctgaaa ttatatggca tgaatccaat aattgcaccc aataatatca acccaactat
     5221 agccataaat tcatcggcaa ccatataagt agcgatgata atgtaaaaaa gcaaaattat
     5281 tatagcagta ccagcggcta agccgtctaa tccgtcgagg cagttatagg cgttagtgat
     5341 gccgataatc cagataaaag tcaatacata ggaaaaaata ttgccaatta tatgattagg
     5401 aaggaaggac aaataaatcc catatttgat taaaattatc gtagccagaa tctgggcacc
     5461 aagttttatc agtgcgggaa cgtgtttaat atcgtcaata ataccaacaa aagctactac
     5521 actgctagcc aaaaaaagcc cggtaaactc gcgcggcggt ttagtgacaa tattaagcgt
     5581 gatgataaag gtaataaaga tggcaatgcc accggtccga ggggtggcat gctgatgaat
     5641 gtgccgacca cccggtttat cggttatatc cataattaca ccgagcttgt agaaaatggg
     5701 tattagaccg aaagtcatca aactagtaac taaaagataa tagattttcg ataaattatg
     5761 gctgaacatc caggttttag ccacataggg taatagaagt gccattaaaa acggtaatac
     5821 gagcatccga aagagaatct ttttattgtg attgttcata attctgctat acgatttaaa
     5881 atttcagtaa ctcgatcacg atcttcattt gaaatcgccg ggaaaaccgg aattgagaat
     5941 tgcttttgcc aggcttgctc ggtttcgggg agcgattcag tttgagcata gctgtggagt
     6001 ggcttataaa ccggtttccg gaaacaagcc cctaacttag cggctaaagc aatgcattca
     6061 gcggccgatt tatgaggatg agcggcaaca cagcgataat tgttcgattt aagccgcgaa
     6121 ttttttaagg gtagtacaaa ttcgattccg tcaacttttt ggtattgctg aataataact
     6181 gtgcgtttgg tgaaatagga ttggtacttt tttatttgtt caattgcaat agccgcaccc
     6241 aaatcggtca ttttagcatt aaagcgtggc tgtaaatcgt ctttttcatc atattcacgc
     6301 aagtcacgga ctttctccaa aattgctggc gaatcggtaa ggatagctcc gccttctcca
     6361 gccccgatga ctttggtcgc ataaaaagag agtacgccaa tgtctccaaa atgaccagct
     6421 ggctgtttgt ctatagtagc accataagcc tgggcgatgt cttcgattac tggaatgcca
     6481 tattcaatga cctcttggat atgaccgggt tggccaaaca tatgtgggaa aatgatcgct
     6541 tttgtgcgag gtgtcatttt taatttgact gaaggcggtg ccagattata agtctcatct
     6601 tctatatcgg cataaattgc cgtcgcaccc acagtagtga ctgcttgcca caaagccgca
     6661 caagtataag atggaataat aacctcatcg cctggtccga ttcccaatgc ctttagggac
     6721 aaaatcaaag ccatagttcc tgaagatact gcacaggcat attttcgatc gagattttcc
     6781 gccaaaattt gctccagtag attcacctga ccattttggg caagctggcc gctccggatg
     6841 acagatgtta cggcggtgat ttcttcttga ccaaaggttg ggcgatagtg gggaatcata
     6901 tatctataaa taaacggagt gaatgaaaag cttcggcgca ggcgactcgt gcctgcacgc
     6961 ctcgaaagtg agcactggat tcggcaactt gaataatcga aagattagcg atatttgtgc
     7021 gattgatttg agaagtatga gccaacaaag cccttaattt gattgctaaa tattctgtgc
     7081 cgatatctac gaaaacgttc ggcataaaat tttgagttgt gggggtttcg taaaaaagta
     7141 cgttgggaat atagcgcgtg gcggaattgg tgatcgcggc tatattgcgg tgatcctgat
     7201 gggtgtcatc gtgataatta accagaatca tatcgggatt cactttatga atgatttcct
     7261 cgagtgcggc tatggaattt ttattgattg ttatatctgt atcttcaaaa ctaccccaga
     7321 aaatatcttt gacgcccatt atgcgggctg aattttcttg ttcggcccgg cgcacttcgg
     7381 gatcgccgcc tgcgcctccc agagtggcta catacaggta aactgaatgt cctttggcat
     7441 tgaatttggc taagctggca ccacatccga tttcaatgtc atccggatga gagccgatgg
     7501 ctatgatatt catctttttt tctgatgagc tcatcgaatt cccccttgga taatagaatt
     7561 ggctcgttga ccttcattaa ataataaatc gagaactgac atatttggta ggaaatcatt
     7621 agtcatttgc cgataaacgg gatgctcgaa cgattggaac ttaagtttga tgtcattctt
     7681 tgcaaaaagc tcaggttgta ggtaattaca gccaccgatt cctgagagat agcaattagc
     7741 cccgacggct ttggtagcaa ttatcagtcg ttcggcggca ctgatctggg aattttggtt
     7801 gagagctttt atttccgaag caattactgt cggagtatca attccgatta aagacattaa
     7861 taagcgaatt gcagttatat tgaactgggc gagattttgc cactcgaatt gataaagatt
     7921 ttcgagcttc tcgaagtata acttgaaaaa tggagcccgg ccgtaataag tctggagagt
     7981 ttgaagatgt tttttgcgcc aattgaccgt attgttgatt ttgacctcgt ctattttttg
     8041 accaaagtga tgaattacag gaaccgtcag ccaaatccag ccttgcggag tgcgaatttt
     8101 attgcgattt tgccattcat tttttttgaa ctggacattg tcaaggaaaa tgaaaatgtc
     8161 agcctgcata attttggcaa aataacccag ccagggataa gtttgcggtt gatgaatggc
     8221 acagatcatt aaatttcctt gatttttatc ccatttttac cttagtttgc caatgatgaa
     8281 aaaattagga gctacaatgg atacaatcat taaaggttgc attatcgtcg gagtcatttc
     8341 cttaatccta ggcatagttt cacgtcttac tttgaagccc ttttttgttg aagcgcaggc
     8401 atttatgcag ttcgcgcagt tttgtttctt agccgcaatc acttttttac tttataaaat
     8461 agcctataag gttaaggact aacactccta tcattattta ctaacaaaaa tcgggttgct
     8521 ggcgagagtt agccagctgt cgccgccata aatccggtaa tagtacatcg gattcggcat
     8581 cggcgactca tctacgaacc attcatctaa aacagtattc aaatggaaaa ttttgatcaa
     8641 ttcgctattg cgatagagaa aaattgtttc cggtttgttc agcgtagtta gtgctcgcaa
     8701 tcgaatatgg atggcaactg gcggtcgcgc atttgtcaat gtttctccga taaaggcatt
     8761 attgccggtg tgcagatcat aaacggaaaa atcatcaatg acgattttat tgccagtgaa
     8821 actgcgcgtt gcatacatcc gtccagctcg catagcttcg taaactccag cccgcgaacg
     8881 ttctttgaca aataagaaag ttaaggtttc agtgacattg ttaaggttgc tggaatcctc
     8941 gaaatcgagt tcgccgattg cccagaccgg tttgcgacgt aagcctgcgc aatattcagc
     9001 caatgctatg tcccacaaac cgcccggtcg gccgatgact tttgcccctt cccagaaaat
     9061 ggcaaatccg gtataatcct gggtttccat aatgcgatga ggataagctt cggtggagat
     9121 tttaattgcc tgattcatta gggggatatt aaccggccga acttcattat gggtgacttc
     9181 cgggtgagcc cagaaaacca gaccgttatg ggcattgacg taatcaatta gctcctgata
     9241 aggagcactg ccagctttgc ggtcgtaggg tgtatattta cgcggcagga acggatattg
     9301 attatataaa atcagtccaa atataatcgc cataataaaa gccggaaatg aaaagcgaat
     9361 attttggcgc ggacgattga tatttttatg gctaaataat ccatattttc gtcggaaaat
     9421 taacgctaat gatattagaa ataataaaaa atagagtgtc atcaacgcaa agtgagtaaa
     9481 attttcgccg atatacttaa taattttacc atcgggtttg ctataaccga tattgtgatt
     9541 agccagcgaa ggtaattgtt ggaagacttt tggatcttcg agaccgaaaa cgagtaagtg
     9601 agtatgccaa tctcttagaa ccatattctc ataaagcggc gaaccttccc agcgatagta
     9661 aggaacagct tcaatgcccg gaattaaaat caagttagga taagccgcgt cgagagcgtt
     9721 gatttcgttg atatatttct ccactccgta gcggtgaacg gaattttctt caatcgtaaa
     9781 tttcaaaaga ttctcaaagg gtcgtatgcc gtaagagact ttcatattat catggtcggt
     9841 tatgataccg acgtctaatt tgtgacgagc gattatatta gccagttccg ccagagaata
     9901 cttaccgccg ctgacttgcg aatcgaaatg cacaccggtc tttagtaaaa tataaccagg
     9961 cttagtctga gaaaagacga gagagtcagt cattattagt aaactgaccg ttattaaaaa
    10021 aggtgcgaat ttcttattca ggcagattcg tgttacacgt ttgataagca taaataactc
    10081 gcttaatata attatcaatc gtgaaatatt cagccttttt tcggtttgta gaagccatag
    10141 attgtcgttg ttcaggattg ctggcgatcc tctgaatggc tgtggctaaa gcttcggcat
    10201 catccgaatt aaccagcaaa ccatttacac catcttgaat taatgatggg acgccgccga
    10261 cgcgcgttcc gataatcggt aagccgcaag ccatcgcctc gataatcaca ttgccgaagc
    10321 cctcgtttag cgaggtaaag acgaagatat cggacgatgc cataatttct ggaacatcgg
    10381 tgcggtagcc gagaaattgg atatttttca aaccaagatc agcgaccagt ttttcgttat
    10441 actgtcggtc tgcgccatcg ccgacaatga tatattccag attcaagccg ctatgctcta
    10501 aaatgccagc cgctctgagg agtaacggca ggtttttgac cggttcaatg cggcccaccc
    10561 aacagatgcg tattttttcg tcgggtgatt tcagtgttga attatcatga caggctttaa
    10621 agaatgggtc caaatcagct ccgccggaag tgacgtagaa tttcgaggat ggaccaatgc
    10681 gttcgcgtat atgatcctgg cgaccaattt cggttaagtt taatactcga tcgcagtaat
    10741 gtgccgaaaa tttttctaag gttataaaac attttgatat ataccggtta taatagccgt
    10801 aaaatatatg gccgtgagtc gaatgcaaaa tttttggcac tctagctaag cgtccggcta
    10861 aacgccccag aaacccagcc ttggaggtat tggtgtgtaa aatttccggt cggtatttta
    10921 ggatcaagcg gtaaatctga agaaaagcca ggggatcata aataggtgaa ggatcacgca
    10981 ctaatgttct cagaaaatac agctgaaatc catttaggcg ggcgtattct gctagatcga
    11041 acggaggatg agtggttcta ccagctacca gcagaacttc atagccgttt tttaccagac
    11101 cggcgcataa cttcagagtg acattagacg aaccgccttg atcaagacgg gttattaatt
    11161 gtagtactct tggctgtcgg ggatttctgt taatttaacg cctctacgac cgaattaatt
    11221 ttttgcagaa ttgcgggacc gatttgagca tgggttggaa ttagaagtaa acgttcagct
    11281 agataagttg cctgcggata attatcataa gtaaatggat ttaattccgg gaaaaagtgg
    11341 tgcagtggtt tctcgtataa acgagtggtt tctatgccaa ccttaagcaa ttttttctgt
    11401 aaacgctggc gctgatccag atttggaaca atgaccggaa attggttgaa cgccgcttgc
    11461 gagttgggca aaattttcgg tatggtgtag cctggtttcc cggtgaaaat ctgcatcagc
    11521 gctttgccat tttcgacacg tcttttggtc aattgatcta tacgccgcca gagagttttt
    11581 cccagagcgg cttgcatatt agtgtaagca aaagaatcaa agtgggggtg caaggtcgta
    11641 tatttgaatt tggcaataaa aggtaacagc agagtgtaac ataatggatt tacagcgatg
    11701 gaaagcccga gaaaattcag ccacattaag aattcggcta tttggctaac cggtggtaat
    11761 ccggcgacta attcttcgat aattggtgaa tagcgttgat ctttccagac aataatgcca
    11821 ccggctaaag tcgagacgtt tttgccccga ttcagcgaat aaaatccgaa tggcgcgatt
    11881 gagccggtct ggcgaccacc tatggttgaa ccgagtgagg aagccgcgtc ctcgacaata
    11941 aaaatatcct gtcctgcgct ggattcaatg atcggagcta catccatcgg cagaccaaac
    12001 atatgcaccg gcataatggc cagcgtttga ggggttacgg cattcaagat tttgcgtacg
    12061 tccatattga aagtagtcag gtcaacatca actaaaacgg cattcaggcc agcggcacga
    12121 attggtaaga gtaaaacagg cgcagtataa gccggtaaga ttatttcatt acgttttggg
    12181 gaagtgcgga tcttcttgaa taattccagc aatataaaaa gtgccgtagt gccggaattg
    12241 accaaagcta gattttcaac atcgaaatat ttttttaatg tttgagagaa attattccgg
    12301 acatggtcgc tctcaaacaa acccagcgta gcacgcgcta tttcggttaa attatagggt
    12361 gttccggcag tcggtatatg caaaatactc aattcatttc tcccatttgt ttaagtagcc
    12421 cagtggaaaa ccaggtagcg gtgagggaat cgccatttgt tcactatttt taatatttag
    12481 tcttcgcatt aggggttgaa attctttccg gcgatgaccg tgaacgttca ggcgtcctgt
    12541 cagagaactg agccgatatc tgaaccataa atatggatta cgactgtttc gataatcgaa
    12601 tactatccag ccgccttttt tacagatgcg atgcatttca ataataaatg cttcaatctt
    12661 ttcgaaagga tgaaaattaa gaaaggtatt cagacaaaat acgacttcaa atttttcatc
    12721 ttcaaaaggt aagttaaaac cgtcgccgat tagaagaggt tcgatagaaa tattattatc
    12781 tttaaaaagt gttcgagctt cataaattgc ctggcggtcg tgatcgagtc caaacgcaat
    12841 tgcctttttt gcgtcagtca cataatattc aagtagaaac agaccgttgc cgcaacccac
    12901 atctaaaaaa tgtccattga tttggcgggc aattttaaga gccgcggttc gcacgcggat
    12961 ggaagtcagg gtttggaaag ccgcggtacg ggtctgattt ttgagattta aagtcgtcat
    13021 aaaattttca ttatttctcg gctgatgatt tgccaatcaa aagattgacg agctctctcc
    13081 agtccatatt gtcctaattg ttctcttaga gttggatttt ggatgagaga tacaagagtt
    13141 tgtcgaatgg cggattgatc gttggcatca attatgaggc cggagcgccc gttttcgata
    13201 gcagacgcga ccccgccgaa atcaccggcg acaactggtt tcccgcaagc cgccgcttcg
    13261 agaaaaacga tgccgaaccc ttcgtaatcg cctcgtaagc ggacgtcgtc ataagtctgg
    13321 cgatttaata ggataaaaat atcacataga ttgtagaatt ttggtaattc ggcatcaggt
    13381 atataaccgc aaaaaatcac ctggtcagtt acattcaatt ctttggtcaa ctttcggaga
    13441 cgttcttctt cgcgtccggc gccaacgata aggtaaatga gatctggaaa ttgcgatgat
    13501 aagtccgcta cagcccgaat catcacttcg tgacctttgc gttcatcgag ccgggcaact
    13561 gtcaaaagca cagttttgcc gtttagaccg tatttttcca ttaaagtcgc atcttttgga
    13621 gctggtttga aaaaaccggt gtccacgcct ggggtaatta ttttgaattt ttcaggagga
    13681 atgcctaacg ccataaattc attacaggta aattggctgt tcgggacgat atattctgcg
    13741 ttggatagga aagttttgat gtatttcatg aaaagaggac tgtacccgaa gcggaaagtt
    13801 tcggagccat aaacgtaaat attgtatggg attccgaata acttgttcat taaccatccg
    13861 gtaaatccgg acgataatac ctgcccgcaa tggattttac ggaaatgata tttgcgatcc
    13921 agaaccaatg tccagaatgc gtatagcaaa ccttttagaa gtttatcgcg gattaagttg
    13981 ccggctggaa tacgtcgccg aatagtctgc agtccggttt gagcgtcgaa ctgttcccag
    14041 ccgggatctt tgggacaaag gatatagttg ttttcctgcg gaagaagtcg ccagatttga
    14101 taataatagg ttgagatacc actgactacc ggcggaaaat cgccagggat taaaagggtg
    14161 ttttgattac gcatcttgtt cccccaaggt tatataacca attagataac cactcaaata
    14221 aaataccaaa ctcagtaaat aaaatgctcg gaaagttaca atagttttac atagacgcca
    14281 aacgtaactg ccgatcctaa caattgtaga ccgtttctga gttacttcag ccccatgctg
    14341 ataggctaat tcgacgacga attctgggta aaatttattg acgtaagccg ccattttgcc
    14401 gttgcgataa aattgccgca cgaatttaga gaaagtcggc ggcggcaaat ggtgaatgta
    14461 aagttccggt aatactacca cgcgataacc ggcttgccga atttcctggc gcagataagg
    14521 atcgagtccg cgcggaatta attcgttttc gccgccaatt ttaatgaaaa catcccgcgg
    14581 aataatgcaa cagggatgtt cagccaaatc gctgtcggta atttcggaga cgatcggtga
    14641 agtgcggcgg ggcagttcgc gcattactcg ccgtacaaac cagttggcat tttccggaat
    14701 aagattggcg actccagcca gaccgatgtc cggatgattt tccatctgag ccagcatttt
    14761 tgctaataaa tcgtcagagc ccaggcgcgt gtcatcgtcg aaaatgacca ggtatctgcc
    14821 tgccgccaaa gccgcaccac aattgattgc ccgtccttga cgggtatcgc cgattatcaa
    14881 aattacctca atattgcgga aagtctggcg ttcgatttgg ctcatcaacc cgggcaaaaa
    14941 ttcctgtcga gcggcaccca ttgtagggat aatgatgctg atcaaaggct cggtgacatt
    15001 ttccgggcgg tgaatttcga tgcgcccttc ggatgcgccc ttcggatatt taacgaacgg
    15061 acgggaaata tttccagtat aaggtttact tataatcaca atatttaata agttagagat
    15121 atttgatgaa aatatttgct attattcttt attgattggt aaccatactt tcatttttcc
    15181 gactccccgg tttgaccaga cataataggg aatcaggatt aattcttgat tgtcaacctt
    15241 gcctcttaaa atattgacat tttcagttag aactgcgcct tttgtgatat ttaatttagt
    15301 atcgagtggt acggttatct ctgagattcg gtggttatca atttcttcgg cacaatagac
    15361 gatcggaccg cattcgaaag caactttatt gacattgtca gttacctttt catgagcaac
    15421 gactttacgg acagccagcg gcagggttag ttctatttta tcaccacgag tccatttcct
    15481 agttatctcg atgtaacctt tatcaagcac gaaattttcg tctttcccat ttacgagcaa
    15541 gctgactttt tcaggatttt cagcaatgta tttatataaa tcacccggga ccacttcgtt
    15601 acgagcccag cctggcacac gtaattttaa ggtgaaaatg gtcttctttt ccggattgac
    15661 tataatatta attttgccat cccagggata atcggtctgc tggattatct cgattttttt
    15721 ctggttgaca attatatcgg cattgctgga aatatagaga ttaatataaa cactgtcagc
    15781 aatcgtggca taaatcagtt cgggtacaga agggaggaag cggataatat tggtcgggca
    15841 acaggaacag tcgaaccagg gttgacgcgt gcaagctccc tggttgaatt gatattttcc
    15901 atccgattcg agtggattgg ggtagaagaa ttttttccca tccaatgaaa ttcctgaaat
    15961 gagtccgtta tacagtgtgc gttcgataat gtcataatat tgcgcatcgc cggtgagccg
    16021 aaagaggcgc tgattccagt aaacgctacc gatggcggca caggtctcgt tataagcagt
    16081 gagattaggc agttcgtaat tatcaccgaa agcctcgcca tcgtgtcttg cgcccacgcc
    16141 gccggttaaa tacatttttt tagtgacgat attttcccag attttcatca aagcctcgaa
    16201 ataagccgaa tcttgataaa tagccgcaat gtcggtcatt ccggcataca tatacatagc
    16261 ccgaacggca tgcccgacgg cttcggtttg tttggtaacc ggcaaatgat cctgactgta
    16321 ttctccgtac aatttatgag aagtgctatc gccgcgcagt tctagaaaaa atcgagctaa
    16381 ctcgacgtat tggggattgt tagtaatatg atagagtttg attaacccgg tttcgacaat
    16441 ctgatgcccg ggcggaacgc gtaatttatc cggtccgaag ctagcaacca gcagatcagc
    16501 gtttttcaag gcgattttta ggaaattttg cttgccagta gcccggtagt gagcggcggc
    16561 ggcttcgaat aaatgaccgc tattataaag ttcgtgattg gattcctcgt gatcccagcg
    16621 attcggcgat ggctgagccc accacgccgg tggatgcata cggtcaatgg taaaccaagt
    16681 agtgagataa ccgtcgggct cctgtccgat tttgattata gaaataatgg aatccagata
    16741 agctgcgaga gcgggattag gtcggcttat taatgaatag gatgcgccct cgatgatttt
    16801 atagacgtcg gtatcgtcaa agggcatttt acctctgact gggccttttt tgcgaccacc
    16861 ggcaatcaag aaattttcca tccgaccttc ttcttcgcac tttttaaaag cgtattggat
    16921 agtagtgtcc tgaatgattt tgatcttcgg caaccagaag gcgtcgttaa tttccacgtt
    16981 tttaatgtcc acagctgata taggataatc ggaaaaagtc ggctgtgttt cagcccaaat
    17041 cgggatcgaa gttaaaagta acagacaaag gaatcggttt ggcttattca ttgtattacc
    17101 tcacattaat tacgtgtaaa tttattgtaa aaatatctca taaactcaga atatgagaat
    17161 tgaaatgact cgcactggag agtctttatt atcagccgtt aggcaacggt ttacagttca
    17221 tctgaggaga agcattttcc tgatttccaa aattcccgtt ggtgtcttta actggcagaa
    17281 atagacgcca ctgctgacgt ctttaccgat attgtccttt ccatcccaaa ttgcaatgtg
    17341 agttcctgat gacttttctc cttttgaaag cgtccgcact aattgtccct gaatatcgta
    17401 aatggcaatt tcaacctgag cgctcgtagg aagagtgtat ttgatagaag tggaggcgtt
    17461 gaacgggttg ggatagtttc cgataagttc gaacgtagcc ggagcgggat gagaattgct
    17521 ttcatcaatt cccgtagaag gtgagataaa tgccccataa acatcaccat cagtgaaatc
    17581 ctcatcaact ctagtagtga ttgcgcaaaa acgatcagag gcgaaagaga ccccggtaag
    17641 taatggaaat ttattttcta atggaccaaa tatggtaaat tcgaggtcta acggagtacc
    17701 agaggtattg aagaatcttc ctttagaact tccagctgat ccatcgaacc aggttattaa
    17761 atagttagtt ccgtcaaagg ctatcgcagg aaactgctga tctccagtct catccgcaat
    17821 ggcgattctc tctgcagctg taccggcggt agttacaaag cgcccataaa ggtcccattc
    17881 ttcgttctcc atttcttcat gaaaaactat caaatacctt gaaccgtcaa agccaataga
    17941 tattggattg tcactgggat aggaactgtc gtcaacgatg aaatttccac ctaccaatgc
    18001 gccttgttga cttaccaact gtgcggagat agttctatcg gtgtcaggaa tttcatttac
    18061 ccaagctact agataacttg tgccatcaaa ggctactgca ttctcacggg cactgctgtt
    18121 gatagatatt tctgtcccta cgagatttcc tgatttgtct atgagtcgtc caatcagagc
    18181 accattgtta acccaggtta ctagataggt gttaccacca aaggctatgg ccggagccct
    18241 taaattgtcc tcttccttag cattagtcgt ggctataaga aaagctgttt ccaccagatt
    18301 accggaagtg tcaataaatt gtccataaac ctcataattg gttgaggtaa tctcttccat
    18361 ccaaaccaca agataattcg tcccgtcgaa tgccactaaa ggcaatccag ccggaaaagg
    18421 agccgtagtg gcttgtccta atgaaattct actacctgaa ataccagttt gagatatcaa
    18481 ctgggcggtg atattatatc ggctttgagc gtcaccaaga ataccgatta acccattggt
    18541 actgccggaa gcaccaccac cgcaaaaagc gctttcttca cctactgcaa tcggaaactc
    18601 ggtgagtcct aagttttgag cccccgcaat agagactgtc aaaactgaaa aaaccattaa
    18661 tcttaaagca gctaatcctg tctttaacat atttacctcc tcagaaaact atctgcaatt
    18721 taaaagtcta aattcttatt atcaatgact tttttactct ttcaattacc gatagaattt
    18781 gtcattaaag tggcaaattt tggctgaaga aggattcttg gattttagag aaagcatttt
    18841 aaaggtaaag gaaacggaat gttttttaat aaccttatcg taaactaatt caaaaataac
    18901 tataaagatt caagatataa gggtgattta ggttttctgc ttggcgtgtt tttataataa
    18961 aggaaataaa tgagattagc tacagtgtca ggataataac gatctgatta catccagata
    19021 atcacaaagc aggcgggtaa tgaggaagtt acttctgata tgctctttgc cgttctgccc
    19081 cagtttttct ttcagttttt tatctttcat taattgaatt atccgttcgg cgaaggcctc
    19141 ttcatcattg ggttcgcaaa gaaaacccgt ttcaccgtca atgacctgaa ggggtattcc
    19201 accgacattg gaggcaacta cagcggcttt tttccagagt gcttccgcta ctaccaatcc
    19261 aaacccttct ctgatagatt tctggattat gacgtcggaa taacgctgaa gagaattgac
    19321 gagaatgtta ttttcggagg taatgggaat aatatccccg gattggatta gatgattaga
    19381 atagtccaca actttctgat atattttcat accttccggg tcgtcagtag ccatactgcc
    19441 gcataaaacc aaacggcaat caatctgttt tttgactttt ttaaaaattc ttacgacgcc
    19501 ctccgggtct ttccacttat cgaagcgcga gatttgagta agaagcggtt tatctatcgg
    19561 tattccaaat ttttttaaaa atcgcttggt atccttttcg gacaattctt tatttttagg
    19621 gctaagtggg tcaatcaccg gtggaataac cttttgttcg atataaatat cgtctttttt
    19681 ataagtttca tgagatacaa ttgctaaatc gtatttgagg ataaacgcct tcagaaaatc
    19741 ccataatacc ggattgggat gcgaaatatc aacgtgacag cgccaaatcc agggttgttg
    19801 tttgacgtag tatctaatca acgggagcgg ttgtgggtcg tgaataatga cacaatcgtg
    19861 atcgagatga gtaaaaaccg aaaagtcctc gctggcttta atgtaaattt cttttttcat
    19921 atcgctcagg ttgatcggat cgccctggag tgcattatgg aattttttgg ttatggtaaa
    19981 taaatcggga ttgcctctga tgatccgcca accagtatcg agaccgacgt cgttcatcaa
    20041 cggtacgaga ctgtttaaaa tttcagccac tccaccgccc atataggtag aattcaggtg
    20101 gataattttt ttgccatata agccccgtgc tttttggaat atgccagtaa ttactttatc
    20161 gccgacaatt cctcgatagt caattaattt cgccattttc ttatcccctt aattatagtt
    20221 taggcttatt atgaattcca atttatcgag actaaacttt aatatgatat tactctccat
    20281 ttcttagttt ccattgactt tcaacaagtt cttgagcaat tcaataactt cggtatgatt
    20341 atagagacta aacctggcat gagtttcaac cattccaact tttattgaat aagctctttc
    20401 gggaagcact tcgaataggt tttcgtcagt ccagtcatcg cctattgcta atataaaatc
    20461 aaaatccttt ttcccaatcc attgaagtcc agcggcaccc ttatttatgc ctgcattagt
    20521 aatttcaacc acttttttcc cctgttgcac ctgaatatca atattggcga taaaatttaa
    20581 taagtaatcg gtgagttctt ttgcccgtaa agaaccaagt tcaggttctg ccatccgata
    20641 atgccacgcc acggaaaatt ccttctcttc tataaaagaa ccgggtaaac gatcggtata
    20701 aacctcaagg agcggaataa tttttgattt ccaatcattt gttaacggtt taattatgtc
    20761 ccagtctttg tttttctcct tgagccagac gccatgctcc gctaccaagc cgatatcaag
    20821 cttgccaaac cagttttgaa gggtatcttt atcacgtcca cttattagaa cgatctcgtt
    20881 ttttggattt tccgccagcg attgtaaaat ctctaaaagc tgctcatccg ggcttgccat
    20941 ttggggctgg tcgacaaaag gaaccaatgt cccatcgtaa tcgagaatga taagcctgcg
    21001 atttgcttgg ctgaaatctt tgataagccg atttctgata gccgaactca atagtttcgc
    21061 attgaaattc ttttgatttt cttttaagga tagcaaatct tggatgaaat catctgccca
    21121 cttgaccaca tcatagcgtt ttaaacgttt ctgcatcacc tggttacgct ttaactgttc
    21181 ttctttaggc atttccaacg ctatctttaa agcatctgta atttctggca ggttattagg
    21241 gttaatgatg atagcctcgc ctaattcctt agccgcaccc gccatctcgc tgaggattaa
    21301 aaccccggca ctatcggctt tagctgaaat gtattctttg gcaatgagat tcatcccatc
    21361 tcttaaaggg gtgattaaag ctacatcgct gatattataa agggcgacca gctgattgaa
    21421 aggtacaaat ttaaattgat ataaaatagg cgtccagttg atactaccga atctgccatt
    21481 aatttttccg atgagctcat ccatctgtct tttcatctcc tggtagtgtt ctatcccgat
    21541 gcgggaaggg acagctatta aaacaagaat cactttccta tgccactggg gatttttgtc
    21601 aaggaacgct tcgtaacctc gtaggcggtt gaggataccc ttggtatagt ccagacggtc
    21661 tatcgaaagg atgatcttaa aattgcctaa agtcttttga aattcgtttt tctccttctg
    21721 aacttccgga ctatttacgg cattataaaa tttttgaaaa tctattccca tcggaaatgt
    21781 ctcagctttt ataatacgat catttatgat aatctggccc atattatgct cgtaacccaa
    21841 aatgcgtagt acacatctta agaaatactg agtataatcg tagctatgga accctaagag
    21901 gtcagcgcct aaaagaccct gtaatatttc tctgcgccac gtgcccggga gaagtcgaaa
    21961 tatttcaaaa gttggaaaag gtatatgcag gaaaaatccg atagggttgg atatttttag
    22021 tcttaaaatc ttgggaagca acataaggtg gtagtcatgt atccagataa tatcatccgg
    22081 tttaataatt tccatcactg catcgcggaa agcctcattt acccgttggt aatgttccca
    22141 gcattcttca tcatattcaa cataggaagt aaagtagtga aagagaggcc agatcgtttt
    22201 attgcaaaat ccgtggtaga atttttccat cgttttttcc gcaagaaaaa caggataagc
    22261 atcgaaatcg tcttgtagct ttgtgcttaa tttctttttt gatttatctt cgactactgt
    22321 ccccggccag cccacccaga tatgatttgc tctggtaaaa gatgaacctt tcaaagaatc
    22381 tagataggca cttagacctg aaactaagcc tcccgtacta tcttgaaatc ttaaccgacc
    22441 ttccttttct attattgtga taggcaacct gtttgatact attaataatc tcatattagc
    22501 cttcttcata atctaatctc gatgaataca atgtattcga atttttaaaa gttccgctga
    22561 taacggaatt tgggtaaaat gaaacccaga ctttttattt actatcgtgt aatcaaaaaa
    22621 cattcggatt actttaagta tttattataa aaagaaagtg ttttagtaaa aaactcagga
    22681 taggttatca tttcaggtcc ttttgcgcct ccatgttctg cttcgggaac aatccataac
    22741 tctttaggac ccttcagtct cttatatact ttctttgaca tccagaccgg cgttctatca
    22801 tctttttcgc cgactattaa aaagacgggc gttcttacct tattcgcagc ttttagcgga
    22861 agtaggttct caggataatt ttgtggaatc acaaaattcc tattcgggct gactttttgc
    22921 aaattcgtcg tgagatcatt gagcgaagta ataagcgctc ttccgacata ggcagaaata
    22981 tcatctcttg aggcaatcat tgcaaatgag aggtaggcgc cagtggaaaa tcccattaag
    23041 ccaatttttg ccgagtcaac ttccggttgg tctttaataa aattaatagc cgcattgtaa
    23101 tcggtaagga actccgcgca gcaaagctgg tcttgatcaa ttttccacgg agcactttca
    23161 ccgaaccccc gccagtcaaa agtaaagaca gtataaccat tggtaaaata atgataggca
    23221 tagaatatcg aaaaggccat attacctgca tcaccatcgc atataataat ggtgggatta
    23281 tggctattag aataatgata ttcttttctc tccggtttaa gactgtcggg catcgccatc
    23341 attcttccga taaaatcttt agaaagtcca gtggtatcct gaggcggata gagccatcct
    23401 ttcaaaacca atccgtcatc ggtcttgaaa gaaacctcgc ggtagatgat tccaaaatcg
    23461 gaaggcacac acggatactc caaaagaggt ttcattgcat aaagagattt tgaaaaaaga
    23521 atagcgagga ttagaaaaat tacgaatttt ggtggtttca caggtactat catttgataa
    23581 tattaaagcg aagtattctt aagtgtttaa caaatttggg ggtaagtttc ttggagtctt
    23641 cgaaatataa ctgatgagtg ttttcaaaga aatctggatt gtggcaaatt accagatgat
    23701 gtagctgaga gcaatgtttc gaaaggctag ggcggaagcc tgaagcgtat atgctgtgat
    23761 aggaggtagg tataattaaa tcactcccca aacaatatgc caaactacat ctgtccaaaa
    23821 atgtatccca accgcagcca aaaatccaaa tttcctgaag taataggcag caaaaagcga
    23881 aactactcca ttaagaagaa taatctcacc cataagagca agtggaatct cactgatagt
    23941 attcaaacca aaaagaatca ttaccgaggg aatatgccca aaagcgaatg caagagcgga
    24001 gaaaatggct ataatccaaa atatctgatt ttgccatctc ttttttagga gcacgtaaga
    24061 gataagccat acccaaaacg aaatgaaaaa caggcggaat atcagctctt ctccgatgcc
    24121 agcaacaacc gaggcgacga gagaagtagg aaacggtgga tgaggaagcg accccaatga
    24181 gtggaattgg ttcaaaatta catcaacaag aataaaaaat acaccgatgc ttaccccgat
    24241 gagtgcggga atcaaaaacc tttgtttgtt ggaaattttc gaatcccaaa gattggcata
    24301 gccaagtttt tgggacagct ttaatccaag aaaacccaag ccaccatata gaataagcat
    24361 tatagctgca tttataagag cgagcgctga tttgggagct ggtagttctt gtgttggcaa
    24421 gaaaacacct tggggcaaaa atacattaat agctgtcaag atggcaaggg ttataatgag
    24481 gccgatatag attttgattg ataatgatgg ttttctcatg atttattttg ccattctaat
    24541 cgccatttca tataacgttt cgtggataaa taaagttgcc gatcccttat ttaaaattat
    24601 caaagtgcct cttcaatgac ttttccaaag gggaataata attgttcgct tcttccgtat
    24661 aacttaaaat accattttct ttttgaaata ttcaacagag gctaacaact tcgtccactg
    24721 ttaattaggc atagattttt ttaatatttt tttgaaattg atttcttggg aatacctatt
    24781 atccttttat cagttttttc aattctgtag aactatattt caattttttg catagaaaga
    24841 tacgataatt atatttattc actttatttg catatttgtt ttcaacaata gcaatttccc
    24901 taacatcgtt gaaatattct tcccaatgtt gttttaacca attagattca cctattgcaa
    24961 taataaccgc atctttatca aattcaggca tccactcaaa tgaactgtga agcgaaaaag
    25021 cctgcggtaa accatatttt tctccaagca aatttactgc tccagccatt gaataatgcc
    25081 gtcctaaaat aaaacatttc cttttttctt ccagactcaa atcggtatat gtttttttta
    25141 caacacctag tatttgattc catatttctg ttgaataata attatcaaaa tccattggaa
    25201 tcttaccatc tttattggat tttaggttat atagtttaat atatctatcg agcttaataa
    25261 tcgggattcc ctgaggtaaa agataagtac ctaccaatcc aagcacaatt aaataagaaa
    25321 atattatcca ttttcgatta tgtaaaagtt gctcgtaaaa aaccgcacct aatgggatta
    25381 gaccaagtac gacagggaaa aaataataaa attttccttt ggctataaac aataaaataa
    25441 aagaacataa taatgcgaaa gaaattatcc tgtattgttt atattttgag ctgaaaggga
    25501 taatcaatag acctgtaagc cagaatacag tggtgagagg atttgaaaaa agaacaagct
    25561 tttttaattc ttttcccatt gaaagttgat ttaattgtct atcgtataat tgggaaaaat
    25621 gatgaattac aggaaattgg tttattattt gccagtaaag gttgactgcg atcaaaataa
    25681 aaaatatcag gagtcccacc cataaactct ttttagagaa aatgtctctc tggtagatta
    25741 ctgcagaaat tattactccc acaaataaaa acgcaatgct gtattttgtt agaaatccaa
    25801 gtgcgccaaa tataaaaaac aaaattaagt acttactatc ggtgaattga gtgtaattta
    25861 taatacaata tatacataaa acccaaaata actgctcaaa tgcagtaggt aaaaacagga
    25921 gctgagttcc ttccattcca ggcgcaaata agatacataa taaagtagtt aaaacagcta
    25981 accacttacc tcccaatctg attgttgtca aaccgcaaaa cactattatc attgcagaag
    26041 cgataaggtt aaaaaggtga ttaataaata cggaatcaga ttgaaacaag ttctgtattg
    26101 cggcgagtat ggcaatcata ggcggaaaat ccgcgtaacc aatggcgaga tgtttgccgg
    26161 cctcaatatg taaaaattca tcactaaaaa atccagaatt ggacaaagcc attatttgta
    26221 gcattaattt aataattaca aaaaaagcta taacaatata ttctggttta taccttttaa
    26281 aaaacatatt tgaaatcaat tttttaattt tcaatactct tttatccttt gaaaaacttt
    26341 agatcagaaa tccaccaaag ttaggcacaa gattactaac ataaaaaaaa ttctgccaaa
    26401 gacaaaattt ggggagtgaa acgtagtctt ttacactgcg ttagtcgaag tactattaat
    26461 agaagaaata aaccgggatt cacactctat cacaagattt tcgagtttga gtatgtgctc
    26521 cttgcagatg gtaacaacgt ccttttctgg caattcatcg aaataccatc gccactctgt
    26581 tttcacttca gtcttagctg gtgcaagtgg ggacgtcggc tttgatgcgc ttctctcaac
    26641 aagtaccatt gcattaataa tcagtataat cacttcagtt aaaatgaaaa tccacaaaac
    26701 ttattctctg tcatctccta aacaatatag agaatactaa cttaaagaga aatatgagaa
    26761 acagaggatg gctctctaca tcaaattcac tcaatcttat tccatgttaa tcctgttaaa
    26821 ctggtcagcc gtctaaaaga tagctaacca gttccttgag atattgcaag gtctttcttt
    26881 gagtctctaa tttgatatgg gattaaaagg tgtttaggaa tcttccataa tctgcaaaat
    26941 agaaaaaata ttttgttgtt taatacctct attcataagg tggcagattt cgctattgct
    27001 tttgatacgg gttactctgg gcataggaaa cttctctttt gttagctgca gtctggggcg
    27061 tgtttttgta ttcgatattt tcttctattt gaataacttc cgtcctaaaa aatcggaaag
    27121 tccgcctatc gctttttgaa tggggcctaa atgaatatcg taataataat cagacttaaa
    27181 ccaacctttt tctttataat actcataatc atatagtttt aaatctgaac tttgaagtcc
    27241 tgagcgtgtc attctaaatg tcattatttt aaacaatgaa ggtttaactg ctttttctct
    27301 gtttaatctt ttataaaatt ggtctgatag gttcttgata ttcgctttta atttttcaag
    27361 ctgtttttcc ggtattggct caggggttat aaccgttgta ccctttagca catcaaaacc
    27421 caaattgtct cctacgcttt ccagatattt ttgagtttgt ttcccaaagg ggataaactg
    27481 tgttccaata gctgtgaagg tttttttgaa aaatctcggt ctgtgataga taaaagcaat
    27541 ccggtcaata aaatttttca ttctccccga aacttgaaat gcataacttg gacttgcgaa
    27601 aacaacacca tccgactctt ccagttttgc tataagtgca ttacgatcat cattataggg
    27661 acagaattgc tcacctttat caaagcataa cttgcacccg ctacaaaact cgagattata
    27721 gtcgttcaaa aaaacatact caaaatctat gtctctgtat ttttttaagt ttttttcaaa
    27781 ctcctgaaca gctaaatagg ttcctttttt tcgagcactg ccaataaatg cggtaatttt
    27841 tttcataatt gtccttttta tctaacattg tttaagttga atagtgttgt tatttgatag
    27901 aaacacctaa tttcagctaa catcttacga atttgtggcg tcttaactgc attcattttt
    27961 ataaattgtc gaaaatgcgc tgttatacga agtggaggcg taatccattt tctatatttt
    28021 tttcccaata taaaacacat acccataata ttgtttatat tttgaatata actctgcctc
    28081 acgcctcata aatccaataa aatcctcaac ggttctattg cccgcatatt ttttcataaa
    28141 ttcctcttgt cttggttttt gcggaataaa ataattatct atccaacaat tttcaggcaa
    28201 tataaatgtt gccacaggaa catatcccgc tttttgcatt attaaaatat tttgccctat
    28261 ggtactaatt tcaggaaccg catcaaacca ccatttttca atttcagccg ggcgttcgtc
    28321 agtaaaccat gactcatagg ttacggcgat atagccattt tttttgagaa agcctttcca
    28381 atactttaaa cctttttcaa aaccaatatt ggcaatagct ccttcagacc atataacatc
    28441 aaattcttca ttttgaaacg gtaaattatc cattgaaccg acaatgcctt ttactctatt
    28501 ctgcaaaccg agtttttcag cgtttgtatt aaactttcca ataaagtctg gaaaaatatc
    28561 aagcccggta atagttcctt ttgtattttg ggcaagaatc aatgtctgac cgcctgttcc
    28621 gcaagccaag tccgcaattt ttaattcatt tgaagaattg tcaataaagc ttaatgccct
    28681 gatagtagat tcagggctgc caggtccctg cctatctaac tcgataaaat attcacttat
    28741 caagttaaaa tcaaattcgt gaatcgttgg tttttcgttt tccatattta aactccttaa
    28801 tccaatgtag tatcaattat tctttcaata tattttttac caattttgcc atcattttgt
    28861 ctaacggttt cagcataaaa gaagtcctgc cctggcggga tttggtagag taccgattta
    28921 tcggcacgaa tcttttaggc tgtgttaggc gaagatgtgg tgtcatttta tctttcttat
    28981 ccaaaaactt cccggccacc tctcgtctct tttgtatgaa ctgaaagacg cttccagttt
    29041 atctggatgt tttaagtgca tataacttcc agcccatagt actttaaaac tgtcattgaa
    29101 ggccaaaaat gcttgaagta aatattgctc ggtccaaaat atgaaatcct ttaaaaccca
    29161 ctctctagcc tgaataattc ataaaaataa agatgtttgg ttgtttaata cattgtaatg
//
LOCUS       DAOSMP010000028        28529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_1658_length_28529_cov_20.634438, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000028 DAOSMP010000000
VERSION     DAOSMP010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 28529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28529
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1658_length_28529_cov_20.634438"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..1635)
                     /locus_tag="PKX09_06125"
     CDS             complement(184..1635)
                     /locus_tag="PKX09_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOU17435.1"
                     /translation="MKQLKLDLSNLDNVFPENFTPEQIAQAKTLFFQKLSEGAHQFYG
                     GKIQIAPRAGFFGFNWFNVWYTPGVSRISTTIRDDNDLSFELTNRGNLVAIVSDSTRV
                     LGDGDVTPPGGLGVMEGKAFLMKYLGGVDAVPLCINSRDQNGLPDPDNIIQFVKMCEP
                     SFGAINLEDISQPNCYKVLDVLSEECEIPVWHDDAQGTACVTLAGLINALKLAKKEIG
                     EVKITMLGAGASNTTIARIIIAASGDPQKIMLFDSRGGLHRNRTDIKEDPRFYRKWEL
                     CQLTNPKCVNDFAESCKGADVLIALSKPGPNVIKPEWIKSMADKAIVFACANPIPEIW
                     PHAAKEAGAFIVATGRGDFPNQVNNSLGFPGILKGALIVRARKITDSMAIAAAQALAD
                     FAEKRGITPDNIIPKMDETAVFPSEVAAVAKQAVQEGIARRIITAESAFQQAEVDIQT
                     SRAMFQTLIKESFIKELPESLLVSALEWAISQV"
     gene            complement(1657..2214)
                     /locus_tag="PKX09_06130"
     CDS             complement(1657..2214)
                     /locus_tag="PKX09_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HOU17436.1"
                     /translation="MSDIYHLSTPLQNTDLINLKIGDKVLISGKIYTARDVAHQRLVK
                     LMEISQPLPFDIKGTVIYYAGPAPARPGNPIGSVGPTSSYRMDKFTPPLLSAGVKATI
                     GKGERSQDVIDAIIRYQAVYFVAVGGAAVVAAQAVKTARIVAFEELGPEAIYELTVVN
                     FPVFVAIDVNGNDLFRVGVARYRQE"
     gene            complement(2222..3070)
                     /locus_tag="PKX09_06135"
     CDS             complement(2222..3070)
                     /locus_tag="PKX09_06135"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOU17437.1"
                     /translation="MKNIEVAQIIPVVKKLCINANYYVAADVLAKAQASLQSEKSPIG
                     REIFQILIQNYELAAQWQMPLCQDTGVAVVFVELGQDVHLIGGDFEQAINEGIRQGYS
                     EGYLRKAMVSDPVVKRENTGDNTPAIIYTEIVPGSSLKITVAPKGGGSENMSEVRMMN
                     ASDGLESVKDFIVDCVRRAGGRPCPPVVVGVGLGGNFEQSALLAKKALLRPLSEKNPE
                     PFWADVEQELAERINRLGIGPQGLGGSTTTWAVSIIYKPCHIASMPVAVNLDCHCHRH
                     ETAIIE"
     gene            complement(3221..3574)
                     /locus_tag="PKX09_06140"
     CDS             complement(3221..3574)
                     /locus_tag="PKX09_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOU17438.1"
                     /translation="MSMQLSVVLRTAVMLVFSNLFMTFAWYGHLRNLSDKAWYVAALV
                     SWGIALFEYLIQVPANRLGFTQMSLAQLKILQEAVTLTVFIPFTVFYMKQPLKTDYFY
                     AGLCILGAVYFIFRS"
     gene            complement(3605..4132)
                     /locus_tag="PKX09_06145"
     CDS             complement(3605..4132)
                     /locus_tag="PKX09_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOU17439.1"
                     /translation="MLTIPFEKLIVKTHALWDEQNLVLASGDFKARHFNFMTVGWGSL
                     GTMWSLPFAQVVVRPTRYTYEFMEKYETFTLCAFAAEYHKKLQIIGSTSGRRTDKVKA
                     SGLTPCAARIVAAPVFQEAELQIECRKIYWSDMEPSHFLKDDIAKNYNGHDYHRIYFG
                     EIVAITGTEKYLARD"
     gene            complement(4250..5863)
                     /locus_tag="PKX09_06150"
     CDS             complement(4250..5863)
                     /locus_tag="PKX09_06150"
                     /inference="COORDINATES: protein motif:HMM:NF015177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOU17440.1"
                     /translation="MAGRKYAQRNSPATALKSSFFTILIFLISLNSCSDLYQNQIPDP
                     ILPDYQGIRNMYWKIWQIMEQNIKRGNRENNFSYHYVNCESELLINQSSTIMLALFGV
                     YGSSHFPVMQSLDNFYRGQRSDGFISRIYNSMTGEYLHAPSAIEPMINPPLFTWPELK
                     YYYLTGDQSRLRKYFKIWEKYFEWLDNFCQAKSEAIGLYYNSQNGSLMINSPRTDNDF
                     GGWADMSCQMALFAEQLAETAEIIGEDTRVIYYQNRYQKITEVIREKLWHDEDGFYYD
                     LTSDGHPIRIKTTAGFWPLIAGIPSSEDAPELINHLKNDDEFARRHLFPSLSADEDGF
                     KPNGAYWRGGVWGNENYMIIAGLKRYGEYEFATTTAWNHITNMEKIFTGYFPDTSLID
                     EKYRAHCNATVWELYSPENDQPGTRWDTYNLCRPNYIPTSGFGPVAMLIEDVLGFNVN
                     GPDDQLTWRLWLLNKHGIRNLKFGNNLVTLWCEKRETKESPVDIKGLTSSPFSLTIIL
                     EQDTVTIKQPRGRINISLKMEDFRKIHND"
     gene            complement(5815..7020)
                     /locus_tag="PKX09_06155"
     CDS             complement(5815..7020)
                     /locus_tag="PKX09_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU17441.1"
                     /translation="MHYRQIKKNIRPETRPAFKAAEKYYEKKKYFQADQSYIETIQRG
                     EGFYGNWGRACVARQEKRISDAEKFYTTAFTLNDSLEGFLYDYAIFLQYNSQDWNKIE
                     KVCTKLYTVTKDDEALSILIETAARLKQQSAALKSLEKIARRYPLRANAQVYYASILY
                     DCGQKAEAVRVARDAVQITYDPFQLKLLVTILAQEGYFVESAKVCEKMSRIAPRSAQT
                     LEAWGFLEFQQGHYENAATYYKKALNRDYRIPTLLSLARIYTFYLNEPSKAMYYAKAI
                     TKLERNNCDALYIMAEAKRRAGDIHGALKYSARLIELQPEHPQTYFYHGKLLFQNKDY
                     KNAVKYLEKAVQYNPDIKRYRLVLAKAYAGAGMSEKARATYANYLNEPLKNLWQEENM
                     LRGTPPPPR"
     gene            7619..8188
                     /locus_tag="PKX09_06160"
     CDS             7619..8188
                     /locus_tag="PKX09_06160"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOU17442.1"
                     /translation="MDSNIYNYLIIPLFIFLARITDVSIGTVRIILVSKGNKLGASFL
                     GFFEISIWLLAISQVMQQGLNNVASFFAYGLGFACGNFIGITIEEKLALGLQTIRLIS
                     TETVDILTMTLRDAGYGVTVINASGAKGPVHILYVIVQRKKVKDVIRIASEITPDIFI
                     SVEDIRSVKAGFFPETNRNFKWRLWNKNK"
     gene            8230..8751
                     /locus_tag="PKX09_06165"
     CDS             8230..8751
                     /locus_tag="PKX09_06165"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOU17443.1"
                     /translation="MKFYRKSLFIAYLIGFILLGIDFLTKSIANLRLPFQERVDTALP
                     FLSWFLTYNTGYHFIFGNISNQKIWAIFGIALVVVLGFSLSRSLIKEKESKANVIILT
                     IILSLIIGSAGNVLEVLIKGHATDFFLFHPFPWPSNLCDQYINAIIYIMLPITIIKSI
                     LDWRQSKKKMAQN"
     gene            complement(8854..9768)
                     /gene="argF"
                     /locus_tag="PKX09_06170"
     CDS             complement(8854..9768)
                     /gene="argF"
                     /locus_tag="PKX09_06170"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOU17444.1"
                     /translation="MKRDFLHITDFSTAEIWEVFELTKKLKSYLKEGWEYTPFKGKTM
                     AMIFAKPSARTRISFETGMTQLGGHAIYLGPNDIGLGQREAVKDIAQVVARYDDIIMA
                     RLFAHAHILELAEFSSVPVINGLTDYNHPCQVMADIFTVLEKRGNLDNLKVTFVGDGN
                     NVVNSWLELTLRLPMHFVLACPPGYEPDKELLTKAQNSGLSKVEISHEPFEAVQNADV
                     IYTDVWASMGRETEKEKRKCDFAGFQVNLKLVNAAKPGVLIMHCLPAHRGEEISDEVI
                     DGPNSIVFDEAENRMHVQKAIIHKLMVG"
     gene            complement(9794..11158)
                     /gene="hslU"
                     /locus_tag="PKX09_06175"
     CDS             complement(9794..11158)
                     /gene="hslU"
                     /locus_tag="PKX09_06175"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOU17445.1"
                     /translation="MKNRELTPREIVRELDAYIIGQDKAKKAVAIAMRNRWRRQHVKS
                     AIKEDILPNNIILIGPTGIGKTEIARRLSNLAGAPFIKVEASKFTEVGYVGRDVESII
                     RDLTGIAVTMVKNERMAEVNSQALFLANERILDILLPSGETKHHSEQEEIIPKGDEKF
                     NRTREKLRVMLENGEMEDRLIEITVNQGAMPMMQIFGPAGIEEMEMNLSEMLGSAFPQ
                     KKKIQKVTISEARKIFTQEEAQKLIDMETVVREAVQRVEETGIVFIDEIDKIASSGDQ
                     RGYGPDVSREGVQRDILPIVEGSNVQTKYGVVRTDHILFIAAGAFHATKPSDLIPELQ
                     GRFPIRVEMDSLGKDEFVKILKNPRNALLKQYTALLKTEKVELIFDDDAVEEIAQIAA
                     EVNEKMENIGARRLHTILTTLLEEILYKVPDKDIREVHITREFVQARLQNIVRDRDLS
                     RYIL"
     gene            complement(11155..11703)
                     /gene="hslV"
                     /locus_tag="PKX09_06180"
     CDS             complement(11155..11703)
                     /gene="hslV"
                     /locus_tag="PKX09_06180"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOU17446.1"
                     /translation="MQEKPIPRIIATTVIGVRRNGQAAMGSDGQVTIGETIMKHTTKK
                     VRRIFNDQVVVGFAGASADAMALFDKFENELEKYKGNVNRAAVELAKIWRTDKYLRQL
                     EALLTVMNKETMLIISGTGDVIEPDDDIIAIGSGGAFATAAARALLRHTEMNAREIVA
                     ESLRIASEICIYTNNNFTIIEL"
     gene            complement(11716..12336)
                     /locus_tag="PKX09_06185"
     CDS             complement(11716..12336)
                     /locus_tag="PKX09_06185"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOU17447.1"
                     /translation="MRFKSFIWLLILALIIIGAERATVILEEDEQMIVARFTQPTVTP
                     ITTAGVHLIIPFLAKTYIYPNNLIEWQSNVIEMPTKDDKFIAIESFAYWKISDPLLFY
                     ERVRTIPEAENRLTDLLDGSLRDEVARYTLAELIRSSSRRMDVTEIKDILKVPQSRTL
                     FNVKGRRPAIIAGILKNATATLDSLQLGIQLVDFDLKSVNFVRIIE"
     gene            complement(12414..13124)
                     /locus_tag="PKX09_06190"
     CDS             complement(12414..13124)
                     /locus_tag="PKX09_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HOU17448.1"
                     /translation="MAGKKVQFSNKEIEVSSVSYWLIILIALVLVVIVTALMSFYTIE
                     PGETGVVTRCGKVHQINGPGAHWKLPWGIDRLYRVKVNYPYTAEFGTRTTSDSLTALS
                     DNFYLPEARLLTGDLKMVDLQWVVQYSIQDPVAYLFGVENPENMLRLVGAAAMRSAVG
                     DFSFREVFQTERRAIAERAKNYMQEKFDLYKTGIVIQHVQLKEARPALPLSEPDTTIS
                     LKPFNQTDSTDTDSVKKI"
     gene            complement(13156..14544)
                     /gene="rpoN"
                     /locus_tag="PKX09_06195"
     CDS             complement(13156..14544)
                     /gene="rpoN"
                     /locus_tag="PKX09_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOU17449.1"
                     /translation="MQLSQSQVQKLSQELRLTPQQILQSTILQLNTLALEARVLQELE
                     QNPVLEEVNPEISESSEEELQDNPETSQEDDIDWEEILPQNDDYTPKEERDSDQKKTQ
                     FIQPAPKGLVDHIFDQLKLHNLDDEEERIAAEIAWNLDENGYLTLPIENIAFGLGLPQ
                     KKVLKVLKEIQELDPPGLAARDLKECLLIQLNQIPNSDDAIKVISEHFEDFANHRYQN
                     IMKAYGWDKERMQAVQEMITKLNPKPGATLNTLDPQYIVPDLIVEKVDREYIVEVNDT
                     RIPELQISSTYLQMISNGSKIDNETKNYLKRKIDNAKWFIQAIQQRRLTMIRVMKAII
                     ARQLDFFEDPSKPLKPMILKDIADDVNMDISTISRVTNNKYVQLPSGIYELKYFFNEG
                     IKDEDGEEISTRIVKEKLKEIIDNEDKHKPYSDEQLSNLLKQNGYLVARRTVAKYREQ
                     MKIPVARLRRVV"
     gene            complement(14544..15296)
                     /gene="lptB"
                     /locus_tag="PKX09_06200"
     CDS             complement(14544..15296)
                     /gene="lptB"
                     /locus_tag="PKX09_06200"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOU17450.1"
                     /translation="MELNSQPQKKTLRGENLVKIYGKRRVVDSVNIEVSKGEVVGLLG
                     PNGAGKTTTFHMFTGMIKPTSGKVFINEQEITRLPMYKRAQLGIGYLSQEPSVFRKLT
                     VEQNLMLVLQTLGISRKEQKERLEILLDQLELRKLRKRRADTLSGGERRRTEITRALI
                     TNPDFILLDEPFVGVDPIAVEDIQKIVVSLKDRDIGVLITDHNVHETLSITNRSYLMF
                     EGRILKSGTSEFLANDEDARRLYLGEAFKLAR"
     gene            complement(15300..15839)
                     /gene="lptC"
                     /locus_tag="PKX09_06205"
     CDS             complement(15300..15839)
                     /gene="lptC"
                     /locus_tag="PKX09_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOU17451.1"
                     /translation="MKGRFLISTLFAVLFCWGCAEDKSNEPTMIDRQYPDQEIWNSNI
                     TLTKDGQKRAVVHSGHLTKNNDQALIYMDTDVAVDFYDRDGCHLSHLTSQTATVNEHT
                     NDLIAKGNVVVVSDSGFTLFTEELRWDHRKERIISEVFVTFVTPEDSLTGIGFESESD
                     LKNWVIFKPSGVTSRELKY"
     gene            complement(15836..16831)
                     /locus_tag="PKX09_06210"
     CDS             complement(15836..16831)
                     /locus_tag="PKX09_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOU17452.1"
                     /translation="MPKNKSTSEKYLEKARELLRSEAEAIATLPDKVDQNFKEVVEVL
                     FNTKGKVIVTGVGKSGHIGCKIAATFASTGTPSFFMHAYEAGHGDLGVISPDDTLLII
                     SNSGETDEIIQLIPPLKKMRIPIIGLLGRNESTLAKKCDYVISTQVNGEADPLQLVPT
                     TSAIATLAMGDALAISLLAKRHFNRRDFAGLHPSGSLGRRLLTTVADLMHTGEEIPFV
                     KSSDKMPEVLYMMTKKSLGIVGVLDDSENLIGVVTDGDLRRGLERQKDFMEQTALKVM
                     SHNPKWIKKSSLAIEALHLMEEHSITNLFVYNGEQNRKPIGIIHIHDILRYGIMP"
     gene            complement(16818..17366)
                     /locus_tag="PKX09_06215"
     CDS             complement(16818..17366)
                     /locus_tag="PKX09_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOU17453.1"
                     /translation="MNTDINELLSKIRLIIADVDGVFTDGALYIGTDGQEFKRFHVLD
                     GAGISLLKAVNFPLAIISGRKSAATEVRMQELGLGDALYQGNLAKLEPYAEIKKRYAV
                     ADQEIIYIGDDLPDIPLMKRVGLPVAVANALPEVKQFARYITVLGGGYGAVREVIDLV
                     LNAQHLKSKAIGILTGDTYAEK"
     gene            complement(17363..18181)
                     /gene="kdsA"
                     /locus_tag="PKX09_06220"
     CDS             complement(17363..18181)
                     /gene="kdsA"
                     /locus_tag="PKX09_06220"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOU17454.1"
                     /translation="MPEVKVKNIAIGNRQPLAFILGPCVIESRDHALRMAEKLKALSE
                     ELDFPLIYKSSFDKANRSSIESFRGPGLEAGLAILAEVKQQFGLPILTDIHEPFQAEI
                     AAKVADVLQIPAFLCRQTDLILAAGRTGKVINVKKGQFLAPQDVVNIVRKIESTGNHS
                     ILLTERGISFGYGGLIADVRSVPIMQETGYPVIFDATHSAQIPGGNVTGGAREFIPHL
                     SRAMVAAGCDGIFMEVHDNVAEAKSDRATQYPLEDLPKLLKELIELGELVRKFQ"
     gene            complement(18162..19811)
                     /locus_tag="PKX09_06225"
     CDS             complement(18162..19811)
                     /locus_tag="PKX09_06225"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOU17455.1"
                     /translation="MKTDKTTKYIFVTGGVISGLGKGIACASIGYLLKSRGFKVTIQK
                     FDPYINIDPGTMSPFQHGEVFVLEDGSETDLDLGHYERFIDENMNSRNSNTSGKIYYE
                     VINRERRGDYLGATVQVIPHITDEIKRRITAVNVNSAYDVVICEIGGTVGDIESQPFL
                     EAIRQFRFESGRSNCINVHLTLVPYIETSGEIKTKPTQHSVMRLREIGIQPDILLCRS
                     KFHLDESIHKKIALFCNVVPEAVIDAIDAETIYEIPLIFDQQRLGDQITDLLNLPRRK
                     PDLKDLEEFVQAIKNPRDEVNIAICGKYTQLHDAYKSIIESFNHAGAENNTRVNIVWI
                     ESEDLTNDSETVNKLKNVDGILVPGGFGERGVKGKINAIKLARENGIPFLGICLGLQC
                     AVVEFARNVCGWKDADSTEFNENTQHPVIDLMEEQKRVFNKGGTMRLGAYAAEVVPGT
                     LAYEIYKNKQISERHRHRYEVNNTYLAEMVKAGLVVGAVNKELNLVEMIELKNHPWFV
                     ACQFHPEMKSRINKAHPIFREFVRAARKYHNQKAENARSQS"
     gene            complement(19801..20544)
                     /gene="kdsB"
                     /locus_tag="PKX09_06230"
     CDS             complement(19801..20544)
                     /gene="kdsB"
                     /locus_tag="PKX09_06230"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOU17456.1"
                     /translation="MKVLGVIPARFAATRLPGKPLVSLHGKTLIQRVYENARNSQLIN
                     QLIVATDDQRVFEHVKSFGGEGRMTPVELASGTDRCALVARDNEADIVVNIQGDEPFL
                     PAEIIDRTVQSLIDDPTLPVATAACHNITQADLSDSNVVKVLVNKRDEAIYFSRANIP
                     YIRDKSIPLINHPALVHIGLYVYRADFLQKFTSLAVTILELLEKLEQLRILENGYRIK
                     VVRTNQKSLSIDTPDDFKKAQKYLEEYEN"
     gene            complement(20541..20840)
                     /gene="gatC"
                     /locus_tag="PKX09_06235"
     CDS             complement(20541..20840)
                     /gene="gatC"
                     /locus_tag="PKX09_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOU17457.1"
                     /translation="MRVDLKTAEKIANLARLTLSEAELERYAAQMDQILEYIEQLQNL
                     DTSNVKPLSHVQEITNVFRNDEVKPSLPKEAVFANAPQEKAGYFVVPKVIKDINP"
     gene            complement(20951..22582)
                     /gene="argS"
                     /locus_tag="PKX09_06240"
     CDS             complement(20951..22582)
                     /gene="argS"
                     /locus_tag="PKX09_06240"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOU17458.1"
                     /translation="MIEHIRGLLEKALQQNDIDYREPIQIERPKQETFGHYTTNLALA
                     VAKIQRQSPLEIAEKLVATLPQSEYIERVEYRAPGFINFFLSPRVLQQTVAQIILAAE
                     NYGKLDIGKNQRVQVEFVSANPTGPLTVGHGRQAVLGDTVARILEWVGYRVDREYYYN
                     DAGRQMRMLGHSVYWRYRQLLGWKGEFPEDHYQGEYIIEIAREVLNKYGDSLQDDPDH
                     EAFRITAEERIFADIKQTLERLGVVFDNFYNEKSLYENGQVNAVLEKMKAIGASYEKD
                     GAIWFRAGLYGGTDDRVLWKSVVDEATYRLPDIAYHVTKFERGYDLIIDIFGADHHAT
                     YPDVLAGLRALGYDTSRVKVLIHQFVTLIKGGEKVKMSTRKANYVTLDELIDDVGRDV
                     ARYFFLMRGMKSHLNFDLDLAKTESEENPVFYLQYAHARICSILRNAATKNLAVGNDF
                     DMTLLKEEETLSLLNALSEFKEVIAICVSTLEPQHLTTYLQKVATAFHKFYTVHRVIS
                     KDWELSQARLALVKAVQIVMANGLALLGISAPERM"
     gene            complement(22662..23573)
                     /gene="queG"
                     /locus_tag="PKX09_06245"
     CDS             complement(22662..23573)
                     /gene="queG"
                     /locus_tag="PKX09_06245"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOU17459.1"
                     /translation="MSLTAEIIRLAENSGLARVGISPPFLDYKYITFFQQWIDAGFLG
                     TMSWMRKYYDFQNYQKTYFSWAKSVLVVADNYFQNVEWPTGIPKIARYAWGKDYHKVV
                     KQKLAKVSREINLLEPATVSRIYVDSGPVMQKIFAWQAGLGWVGKNNLLIVPDLGSYC
                     FIGMIFLNLELHFNEPQSNLCGDCQLCRDACPNQALVDEHLLDARLCTAYLTIEKDDE
                     FSAAEKKNLRGWIYGCDTCQKICPYNQKWKRPGDTRYSEYYSEFSKSLTDWLKVTEDE
                     FGEIFRDSPIRRIGFANWRRNLLTLRK"
     gene            complement(23625..24008)
                     /gene="rsfS"
                     /locus_tag="PKX09_06250"
     CDS             complement(23625..24008)
                     /gene="rsfS"
                     /locus_tag="PKX09_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOU17460.1"
                     /translation="MKTSQNTTDKNQSYPLARQIADIAVDKKARDVVIIDLRPLTSIT
                     DYFVICTGAVGQHVKAIVDEIDDRLRPAEKPWHIEGYQHLSWVLMDYVNVVVHVFNPE
                     ARAYYNLERLWADAATEIISDDSAD"
     gene            complement(24012..24551)
                     /locus_tag="PKX09_06255"
     CDS             complement(24012..24551)
                     /locus_tag="PKX09_06255"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HOU17461.1"
                     /translation="MARSRNRRKRPTSRKNKRRAVDLQKWFLNLAIFSISVVIVGFIF
                     SMGKRFNNPNEKLTMSQVNVIPPDERPVPYIVIEVLNGTDVPGLAQKFTNFLRQNGYD
                     VISTGNADYSGYEKTMLITRTADVTKLREVNGTLLLDSDQLFQKIDSTLQVDLTLIVG
                     KDYQRLPVYSKIIAIGETF"
     gene            complement(24554..25033)
                     /locus_tag="PKX09_06260"
     CDS             complement(24554..25033)
                     /locus_tag="PKX09_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17462.1"
                     /translation="MKRNFLTVTMLAIFLGTSGFAQLKTQIQRSPTIGEAIHLPGISS
                     AISNYSLFDPSRFKMQQSYSLSYSTFGNQSMSVGVYQNAMSFLLSNKFLLNTRFGFYH
                     NPLQLGTFNATSNNMLDNLIFGADLIYRPKENATFMIRFDRRPYYYPYEYYNPYLGY"
     gene            complement(25119..25967)
                     /gene="panC"
                     /locus_tag="PKX09_06265"
     CDS             complement(25119..25967)
                     /gene="panC"
                     /locus_tag="PKX09_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOU17463.1"
                     /translation="MQILTKIEDIRQELLVIRTNAKKIALVPTMGYLHEGHLSLVDVA
                     KVNADVVVMSIFVNPTQFAPNEDLMRYPRDIERDERLARERGVDYIFHPEVAEMYPDP
                     YFTYVVTEQLAKVLCGISRPTHFRGVTTVVAKLFNIIQPDVAVFGQKDAQQAVIVKQM
                     VRDLNFPIQIIVAPIVRESDGLAMSSRNVYLTPEERQQAPIIFKALQAARENVKNGLT
                     DANQVRDQITKMIQTSPLARIDYVEIIDDQTLTPVETVKPGTFAAVAVYYGKTRLIDN
                     IYLKER"
     gene            complement(25991..26791)
                     /gene="panB"
                     /locus_tag="PKX09_06270"
     CDS             complement(25991..26791)
                     /gene="panB"
                     /locus_tag="PKX09_06270"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOU17464.1"
                     /translation="MKDKVRVSTILEMKQKGVKITSLTAYDFTMAHLMDESGVDIILV
                     GDSCGNVCAGYETTLPVTMDEMVYHVQCVRRGVKRALLVADMPFMSYQESVEQALHNA
                     GRFMKECGAEAVKLEGGEPICPVVEKMVEIGIPVMGHLGFTPQSVHKFGGFGVRGKET
                     EEAKYIEKSALALQDAGAFAIVLEKIPAKLAADVTAQLRIPTIGIGAGKECDGQVLVS
                     YDMLGLFDQVKYKFVRRYANLADEIRRATTAFGNDVRNGNFPDESESY"
     gene            complement(26858..27946)
                     /gene="mnmA"
                     /locus_tag="PKX09_06275"
     CDS             complement(26858..27946)
                     /gene="mnmA"
                     /locus_tag="PKX09_06275"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOU17465.1"
                     /translation="MANDKITLVTKKQKVLVALSGGVDSAVAAWVLCQEGYEPVGITF
                     RMWRMKEDIAQAKAIDRARQVCEILKIPHIIFDLNAEFEEQVVKPFVNEYFQGLTPNP
                     CVNCNRVIKWHNLLKIADQYGIEKIATGHYARLKYSSAHKRYQIWKGVDSTKDQSYAL
                     WQIPQTALKRTLFPLGEMTKNAVKTIAVDHNLIVGKITESQNICFIPEGDYRSFLKEY
                     APQKVQTIGRGELVDERGRVLGWHNGFYNFTIGQRKGFRVGFKERRYVKAINAQKNRV
                     VIAPDKSLFSSKMVIKNVNWVGISAVAEFDGIIKIRYNHPGAMGHGKRINDNEYEITF
                     IEPQRAICPGQSAVVYQDDCLLMGGIIQ"
     gene            complement(27928..28422)
                     /locus_tag="PKX09_06280"
     CDS             complement(27928..28422)
                     /locus_tag="PKX09_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOU17466.1"
                     /translation="MKYHYCYHCGTPTTSLEMEGYRRAFCPQCQLILYENPLPTVVII
                     AYNEQNEIVLIRRAVEPGRGGWSLPGGFIEIGETAAQAAVRELCEETGLSATELKLVD
                     VGTHLNGFYGDILMIGYATPVQEDLIRPGSDAAEAAWFKIDDHPSLVFPFHEKLIENW
                     RMIK"
BASE COUNT         7863 a         6583 c         5703 g         8380 t
ORIGIN      
        1 gacctttggg ttatgagccc aacgagctac caactgctcc accccgcgat caattttaaa
       61 aatcgggcat aatttgcata aaatttggca caataacaag aatcaaaaat tgttttattc
      121 aaacaaaatc acagaattcc attttgctaa aaatcggatt gcttttaatc ggctccctgc
      181 caattagacc tggctaatcg cccattcgag ggctgaaacc aacaaactct caggaagctc
      241 tttgataaaa ctttctttta tcagggtttg aaacatcgcc cgagaggttt gaatatctac
      301 ttcggcttgt tgaaatgccg actcagcagt aattattcgc cgggcaattc cctcttgaac
      361 tgcttgctta gctaccgccg cgacttcaga tggaaaaact gcggtttcat ccattttcgg
      421 aataatatta tccggtgtta tgccgcgctt ttcggcaaaa tcagccaaag cttgtgccgc
      481 cgcaattgcc atcgaatcgg ttattttacg agctcggact atcagagccc cttttaaaat
      541 accaggaaag cccagtgaat tattaacttg attgggaaaa tcgccacgac cagtagccac
      601 aatgaaagca cccgcttctt tggcggcgtg cggccaaatt tccggaatgg gattagcgca
      661 ggcgaaaaca attgctttat cagccataga tttgatccat tccggcttaa tgacattcgg
      721 tcccggtttt gagagtgcaa tcaacacatc cgccccttta catgattcgg caaaatcatt
      781 gacgcatttt ggattggtta attggcataa ttcccattta cgataaaacc gcggatcttc
      841 tttaatatcc gtacgattgc gatggagccc tcctcgggaa tcaaagagca taatcttttg
      901 gggatcgcca ctagccgcaa taataattct ggctatagtt gtattcgaag caccagcccc
      961 taacattgtg attttcactt cgccgatctc cttctttgcc aatttcaagg cattaattag
     1021 accggctaag gtcacacaag ctgtcccttg agcatcgtca tgccaaaccg gaatttcaca
     1081 ttcctcgctg agcacatcca aaaccttata acagttcggc tgagaaatat cttctagatt
     1141 gatagctcca aaactcggtt cacacatctt gacaaattgg atgatattat cgggatcggg
     1201 taagccgttc tgatcgcgac tattaataca gagtggtacg gcatctactc cgccaagata
     1261 tttcattaaa aaggctttgc cctccataac tcccagacct cccggtggag tcacatctcc
     1321 atcaccaagc acccgagtcg aatcgctgac aatcgctact aaatttccgc gattagtcaa
     1381 ttcaaaagag agatcattgt catctcgaat tgtagtcgaa atgcgcgaga cgcccggtgt
     1441 gtaccagaca ttaaaccagt tgaatccaaa aaatcccgcc cgcggcgcta tttgaatttt
     1501 acctccataa aattgatgag cgccttctga caacttctga aaaaacaatg tttttgcttg
     1561 agcgatttgt tctggagtaa aattctccgg aaaaacgtta tcaagattgc taagatcgag
     1621 tttcaattgt ttcataaata ttatctcctg taaaatttat tcctgacgat aacgagcaac
     1681 tccgacgcgg aaaagatcat taccgtttac atcaatagcc acaaaaactg gaaaattaac
     1741 tactgtaagt tcgtagatag cttctggacc aagttcctca aaagcaacga tacgagccgt
     1801 ttttacggct tgtgccgcca cgactgccgc gcctcccaca gcgacgaagt aaaccgcctg
     1861 gtagcgaata atggcatcaa tcacgtcctg actgcgttcg cctttaccaa tcgtagcctt
     1921 cactccggct gacagtagcg gcggagtgaa tttatccatt cgatacgaac tggttggacc
     1981 gaccgagcca atcggattac cgggccgagc cggcgccggt ccggcataat aaatcaccgt
     2041 ccccttgata tcaaacggca gaggctggct aatttccata agtttcacca aacgttgatg
     2101 agcaacatcg cgggcggtat aaatttttcc cgatattaaa actttatcac cgattttcag
     2161 gtttatcaaa tctgtgtttt gaaggggagt ggataaatga taaatatcgg acatattgat
     2221 tctactcaat gatagcggtt tcatgacgat gacaatgaca atcgagattg actgccaccg
     2281 gcatagaagc tatatgacag ggtttataaa tgatactaac cgcccaggta gtcgtacttc
     2341 cacccaatcc ctgcggccca attcccagcc gattgattct ttccgccaat tcctgttcga
     2401 catcagccca aaatggctca ggatttttct cgcttaatgg tcgtaataaa gcttttttgg
     2461 ctaatagagc cgactgctca aaattgccac ccagtccaac tccaacgaca actggcggac
     2521 atggccgccc accagcgcga cgtacacaat ccacaataaa atccttgaca ctttccaaac
     2581 catcggaagc gttcatcatc cgtacttcgg acatattttc ggagccacca cctttgggcg
     2641 cgacggtaat tttcaaagaa gaaccaggca caatttcggt ataaataatc gccggtgtgt
     2701 tatctccggt attttctcgt ttgacaaccg gatcgctaac catcgccttg cgcaaataac
     2761 cttctgaata tccttgccgt atcccttcat taatcgcctg ctcgaaatcg cctccaatta
     2821 gatgaacatc ctgaccaagt tcgacaaata ctacagccac tccggtatcc tgacaaagcg
     2881 gcatctgcca ttgcgcggcc agttcgtaat tttggattaa tatctggaaa atttcacgac
     2941 cgatcggcga tttctcactt tggagcgaag cctgggcttt cgccaataca tcggcggcaa
     3001 cgtaataatt ggcgttaatg cacaactttt taacgacagg gattatctgg gcgacttcaa
     3061 tatttttcac aggatatttc cacttatcca acggttggat ataattttca aagcgcaaaa
     3121 taaaagtaat ttattcatca aataaaacgt aaataacatc cttcatatat ccaatatcca
     3181 agtcacaatt tacataaaat ctaggcaaaa tcttttctcc ttacgaacgg aaaataaaat
     3241 acaccgcacc caaaatacag agaccagcat aaaaatagtc ggttttcagt ggctgtttca
     3301 tataaaaaac tgtaaagggg ataaagaccg tgagcgtcac ggcttcttgc aagattttca
     3361 attgcgccag cgacatttgg gtaaagccca aacgattggc gggaacttga atcaaatatt
     3421 caaacaacgc aattccccag ctgaccaaag cggctacata ccaggctttg tcacttaagt
     3481 tacgaagatg gccataccag gcaaaagtca taaacaaatt cgaaaatacc agcatcacag
     3541 cggttcgcaa aacgactgat aactgcattg acatacctca gttctattct tttctttatt
     3601 taatttaatc tctagctaag tatttctcag ttccggtgat tgcgacgatt tcgccgaaat
     3661 aaattcgatg gtaatcgtgt ccattataat ttttagcaat atcgtccttg agaaaatggc
     3721 tgggctccat atcactccag tatattttgc gacactcgat ttgcaattcg gcttcctgaa
     3781 aaaccggcgc cgccacgatc ctcgccgcgc aaggcgtcaa gccactggct ttgactttgt
     3841 cagtccggcg accagaagtc gaaccgatta tttgtaattt tttgtggtac tcagccgcaa
     3901 aagcgcaaag tgtaaaggtt tcatactttt ccataaattc ataagtataa cgggtcggac
     3961 ggacgacaac ctgcgcaaat ggtagagacc acattgttcc taaactgccc caacctaccg
     4021 tcataaaatt aaagtgtctg gctttgaaat caccactggc aagtactaga ttttgctcat
     4081 cccagagggc atgagttttt acaattaatt tttcaaacgg aatcgttagc attttcttaa
     4141 tctcctttag ttttttgcca ttattctaat aggaaaagtt aatacggtat caaaaaccta
     4201 agtttaggca ttttcaaatc atcttattgg ctgacgtgaa ataagggaat tagtcattgt
     4261 gaattttcct gaaatcttcc attttgaggc tgatgttaat tcggccgcgc ggttgtttga
     4321 tagtaacagt atcttgttcg agaattattg ttaaggaaaa cggtgaacta gttaagcctt
     4381 taatatcaac cggagactct tttgtctcgc gtttctcgca ccagagcgta accagattat
     4441 tcccaaattt taaattgcga atgccatgtt tattcaacag ccacaagcgc caggtcagtt
     4501 ggtcgtccgg tccatttacg ttaaatccca gtacatcttc gatcagcata gcgaccggtc
     4561 caaatccgga agtcggaata taatttgggc gacagagatt ataagtatcc cagcgcgttc
     4621 cgggttgatc gttttccggt gaatataatt cccataccgt agcattacag tgcgcacggt
     4681 atttttcatc aatcaaactt gtatcaggaa aatatccagt gaaaattttt tccatatttg
     4741 tgatatgatt ccaggcggta gtagtggcaa attcatattc gccatacctt ttcaatcccg
     4801 cgataatcat ataattttca ttgccccaga cgccaccccg ccagtaagca ccattaggtt
     4861 tgaagccatc ttcatcagca gataatgacg gaaagagatg ccgccgggca aattcatcat
     4921 catttttcaa atgattaatt agttcgggag cgtcttcgct ggatggaatg ccggcaatta
     4981 gcggccaaaa tccagcggtc gtctttattc taattgggtg accgtcactg gtcaaatcat
     5041 aataaaatcc atcctcatcg tgccagagtt tctcgcgaat gacttcggtg atcttttgat
     5101 agcgattttg ataataaatc actcgggtat cttccccgat gatttcagca gtttcggcta
     5161 attgttctgc aaaaagagcc atttggcacg acatatctgc ccatccccca aaatcattat
     5221 cggtgcgtgg agaattgatc atcagagaac cattctggct gttataatat agcccgatcg
     5281 cttcgctctt agcctgacag aaattatcca gccattcaaa atatttttcc cagatcttaa
     5341 aatactttcg caaccgactt tgatcaccag tcaggtaata atatttcaat tctggccagg
     5401 tgaaaagagg cggattaatc atcggttcta tcgcactggg agcatgcaaa tactcaccgg
     5461 tcatcgagtt gtatatccgc gaaataaatc catctgaacg ttggccacgg taaaaattat
     5521 caagggactg cattaccgga aaatgggaac taccataaac accaaaaaga gccagcataa
     5581 tcgttgaact ttgattgatt agcaactcac tttcacaatt gacgtaatga taactgaagt
     5641 tattttcacg attgccacgt tttatatttt gctccattat ttgccagatt ttccaataca
     5701 tattcctgat gccctggtaa tccggaagga ttggatccgg tatctggttt tggtatagat
     5761 cggaacaact atttagactg attaaaaaaa tcagaatggt aaagaaactc gatttcagcg
     5821 cggtggcggg ggagttcctc tgagcatatt ttcttcctgc cataagttct ttagtggttc
     5881 atttaaataa ttagcgtagg ttgcgcgggc tttttcagac ataccggctc cggcataagc
     5941 tttagctaac actaaacgat aacgtttaat gtctggatta tattgaacag ccttttctaa
     6001 atatttgaca gcattcttat aatctttatt ttgaaataat aactttccat gataaaaata
     6061 agtctgcgga tgttcgggct gaagttcaat caaacgagct gaatacttca aggctccgtg
     6121 aatgtcacct gctcttcgtt tggcttccgc cattatataa agcgcatcac aattattgcg
     6181 ctccagtttc gtaatcgctt ttgcataata cattgccttg ctcggttcat tcaggtaaaa
     6241 agtatatatt cgagctagcg ataatagtgt gggaattcgg taatcccgat ttagtgcttt
     6301 tttataataa gtagccgcgt tttcataatg cccctgttga aattcaagga atccccaggc
     6361 ttctaatgtc tgagctgaac gtggtgctat tctcgacatc ttttcgcaga ctttggcgct
     6421 ttcgacgaaa tagccttcct gggctaaaat cgtaactaat agtttaagct gaaaagggtc
     6481 ataggtaatc tggacggcat cgcgcgccac tcggacagct tcagcctttt gtccacagtc
     6541 atataatatc gaagcgtaat atacttgagc attagctcgt aaaggatagc gtcttgctat
     6601 tttctccaaa cttttcaaag cggctgactg ttgttttaac cgagcggcgg tttcgattaa
     6661 aatggacaaa gcttcgtcgt cttttgtcac ggtgtagagt ttggtacaga ctttttcaat
     6721 tttgttccaa tcctgactat tgtattgcag aaaaatggcg taatcgtata gaaagccttc
     6781 taacgaatcg ttgagagtaa aagccgttgt atagaatttt tcggcatctg agattctctt
     6841 ttcctggcga gcaacacaag cccgtcccca attaccgtag aatccctcac cgcgctggat
     6901 tgtttcaata taagattgat cagcttgaaa atatttcttt ttttcataat atttttcagc
     6961 cgctttgaac gctggccgcg tctcgggtct gatatttttt ttgatctgcc ggtaatgcaa
     7021 tccgtcagga agtttcgtaa aatcgtctga cgccgaaaag gcaagccctg cgaacaaaag
     7081 taaaagtaag ctgataacat actttctcac ggcggaaaat atgccgaatt tccgagcaaa
     7141 ttccaaatta aaatatctga acctttataa tttcactacc ttgaactgca ttgatctttt
     7201 tcaatagctg gttatttatt taaactttta aattaaataa ctcgttccgc tacgattaaa
     7261 ctcattaatg aattcaccaa accgattacc gattgattca tatccagaat aacttttgaa
     7321 ttaacggtat atgtgattaa tttccgattg ttttcgtcgt tggtttgagc ggcgaaaagt
     7381 ccaagcgagt tttttttaat aagcagttga aaaacgaatg actatgcgcc agatcctcta
     7441 ttcgtcaaaa ttttcctctg atctctttca gaaatgcgat aagttttcag ccagttcagg
     7501 attaatttac aattaaaagc aacaaaccaa ctgaaatagt aatagtcaat actttggaat
     7561 acttttaaga aaatatgaaa tttaatgcag gattaagtct attatggcta ctaactttat
     7621 ggattctaat atttacaatt atttaattat tccgctattc atttttttag ctcgaattac
     7681 cgatgtctca attggtaccg ttagaattat cctggtttcc aagggcaata aactaggggc
     7741 ttcatttctc ggcttctttg aaatctcgat ctggctctta gccatttcac aggttatgca
     7801 acaaggctta aataacgtcg ctagcttttt cgcttacggc ttaggtttcg catgtggtaa
     7861 ctttattggc attacgattg aggaaaaatt agccctcggc ttgcaaacta ttcgcctaat
     7921 cagtaccgaa acagttgata ttttgacgat gacactacga gacgccggat atggagtgac
     7981 tgtgattaat gcctcaggcg ctaaaggtcc agttcacatt ttatatgtaa tcgttcagcg
     8041 taaaaaagtc aaagatgtta ttaggattgc aagcgaaatt actccggaca tttttatttc
     8101 agtggaggat attcgttcgg taaaagccgg gttctttccg gaaaccaatc ggaattttaa
     8161 gtggcggctt tggaataaaa ataaataacg accgataaat acgcttgtaa cattttggga
     8221 gcataatcca tgaaattcta ccgtaaatcg ctgtttatcg cttacttgat cggtttcatt
     8281 ttattaggaa ttgatttcct gaccaaaagt atcgccaatc ttcggttacc ctttcaggaa
     8341 agagttgata ccgcgttgcc gtttctatca tggtttctca cttacaacac cggctatcat
     8401 tttatatttg gcaatatttc caatcaaaaa atctgggcta tattcggaat tgcactcgtc
     8461 gtggttttag gcttttccct gtcacgtagt ttaattaagg aaaaggaatc gaaagccaat
     8521 gtcatcattc ttacgattat tttaagcctg ataataggtt cggctggcaa cgtcctcgaa
     8581 gtcttgatta aaggtcatgc gaccgatttc ttcctctttc atccttttcc ctggccgagc
     8641 aacctttgtg atcaatacat caacgccatt atttacatta tgttaccaat cacaattatc
     8701 aaatcaattc tcgactggcg tcaaagcaag aagaaaatgg cacagaattg atcggagtag
     8761 ctacaacaca tcgagaaagt tattagttct cttcggataa tttttaatat tggattatta
     8821 tgaagtttgg gttataaccg acgaataatt tcctcaccca accatcaatt tgtggataat
     8881 ggctttctga acatgcatgc gattttcggc ttcatcgaag acgatagaat tcgggccatc
     8941 aataacttca tcgctgattt cttcgccacg atgggctggc aggcagtgca ttataagcac
     9001 acccggctta gcggcattaa ctaacttcag atttacctgg aatccggcaa agtcgcactt
     9061 acgcttttcc ttttcggttt cgcgtcccat acttgcccag acatcagtat atatcacatc
     9121 ggcattttgg accgcttcaa atggctcgtg agatatttct accttgctta gtccgctatt
     9181 ttgagctttg gtcagcaatt ccttgtccgg ttcataaccg ggagggcaag ccagaacaaa
     9241 atgcatggga agtctcaaag tcagttccag ccaggaattg acaacattat taccgtcgcc
     9301 gacgaaagtg actttgagat tatcaagatt gccacgtttt tccagcacag taaaaatatc
     9361 agccattacc tgacaggggt gattgtaatc ggtcaagcca ttgataaccg gtacagatga
     9421 aaattcggct aattcaagaa tatgggcatg ggcaaaaagt cgcgccataa ttatatcatc
     9481 atagcgagcc acaacttggg caatatcttt gacggcttcc cgttggccaa ggccgatgtc
     9541 atttggccct aaataaatgg cgtgcccacc tagctgagtc ataccagtct cgaaagaaat
     9601 gcgcgtgcga gcgctcggtt tggcaaaaat catcgccata gttttgccct tgaacggagt
     9661 gtactcccaa ccttctttga ggtagctttt gagctttttc gtcagttcaa acacttccca
     9721 tatttctgcc gtcgaaaaat cagtaatatg taaaaaatcc cttttcatta aatccctcag
     9781 gtgaatcaat aaattaaagt atataacgac tcaaatcgcg gtcacgcact atattttgta
     9841 atcttgcttg gacaaactcg cgcgtaatgt gtacttcccg aatatcttta tcaggaacct
     9901 tatagaggat ttcttccagt aaggttgtca gaatcgtatg cagacggcgt gcgccgatat
     9961 tttccatttt ctcattgact tccgccgcaa tttgggcaat ttcctccact gcatcgtcgt
    10021 cgaaaattaa ctcaaccttc tcagttttca gtagagcggt atattgtttg agcaaagcgt
    10081 tacgcggatt tttcaaaatt ttgacgaact catctttgcc gaggctatcc atttcaactc
    10141 ggatgggaaa gcgcccctgt aattctggaa tcaaatcaga aggtttggta gcgtggaaag
    10201 ccccagccgc gataaataaa atatgatcgg tgcgaaccac tccgtatttg gtctggacat
    10261 tactgccttc gacgatgggt agaatatcgc gctgaacacc ttcgcgcgaa acatccggac
    10321 cgtagccacg ctgatcgccg ctggatgcga ttttatcaat ttcatcaatg aaaacgattc
    10381 cggtttcttc gactcgctga accgcttccc gaactaccgt ttccatatca attaattttt
    10441 gtgcttcttc ctgggtaaaa attttacggg cttcggaaat ggttactttc tggatttttt
    10501 tcttctgcgg gaaagcacta ccaagcattt cggagagatt catctccatc tcctcgatac
    10561 cggctggacc aaaaatctgc atcatcggca tcgctccctg attcaccgtg atctcgatca
    10621 ggcggtcttc catttcacca ttctcaagca tcacgcgcaa tttttcacga gtccgattga
    10681 atttttcatc acctttaggg ataatttctt cttgctccga atggtgcttt gtctcccctg
    10741 aaggaagcag aatatcgaga atccgttcgt tagccaaaaa gagagcttga ctattgactt
    10801 ccgccatacg ttcattcttt accatagtca cggcgatacc agttagatcg cgaatgattg
    10861 actcgacatc tcgtccgacg taaccgactt cggtaaattt ggatgcctcg actttgatga
    10921 aaggtgctcc ggctaaatta gaaagtcgac gagcgatttc agtcttgccg atgccggttg
    10981 gtccaatcag gataatatta ttcggtaaaa tatcctcctt tatagcactc ttcacgtgtt
    11041 gacgtcgcca gcgattccgc atggcaatgg caacagcttt tttagcttta tcctgtccga
    11101 tgatgtaggc atcgagttcg cgaacgattt cgcgcggtgt aagttctcta tttttcataa
    11161 ttctataatt gtaaaattat tgttggtata aatacaaatt tcagaggcaa ttcggagtga
    11221 ttcagccaca atttcccggg cattcatctc ggtatggcgt aaaagggcgc gggcggcggc
    11281 ggtggcaaag gcgccacccg aacctattgc aataatatca tcgtcgggct caatcacatc
    11341 acccgttccg gaaataatta acatggtctc cttgttcatt acggttaaaa gagcttctaa
    11401 ctgccgtaga tatttatcgg tgcgccagat tttagccaac tcgacggccg cacggtttac
    11461 attaccttta tatttttcta attcattttc gaatttatcg aaaagggcca tagcgtccgc
    11521 cgatgcaccg gcaaaaccga ccacaacctg atcattgaaa attcgacgga ctttcttggt
    11581 cgtatgtttc ataatcgtct cgccgatcgt cacttgaccg tcgcttccca tagcggcttg
    11641 gccattacga cgcactccga taacggtagt tgctattatt ctgggtattg gtttttcctg
    11701 catattatct ccattttact caattatccg aacaaaattt acacttttta gatcaaaatc
    11761 taccagctga atacccagtt ggagagaatc cagcgtagca gttgcatttt ttaaaatccc
    11821 ggcgatgatg gctggtcgcc gacctttcac attgaacaat gtcctggatt gagggacttt
    11881 caaaatgtct ttaatctcgg taacgtccat ccggcggcta ctgcttcgaa taagctccgc
    11941 aagagtatag cgggcgactt cgtcccgcag agaaccatcc aataaatcag ttaggcgatt
    12001 ttcggcttct ggaattgttc tgactctttc ataaaacaac agcggatcgg atattttcca
    12061 ataggcaaag gactcgatgg cgatgaattt gtcatcttta gtaggcatct caattacgtt
    12121 gctctgccat tcgatcaagt tgttggggta aatataggtt ttcgctaaaa acggtattat
    12181 tagatgaaca ccggctgttg taattggcgt tacagtcggt tgagtaaagc gagccacgat
    12241 catttgctcg tcttcttcta aaattaccgt cgctctctcc gccccaatga taatcagcgc
    12301 tagaatcaat aaccagataa atgacttaaa cctcatccga gacctccgtg agataatttc
    12361 ttgtagaaat caggcgcatt ttggctgttt tatttcactc ctaccagttt tcatcaaatt
    12421 tttttgactg aatcggtgtc ggtcgaatcg gtttgattaa acggctttag tgatatagtt
    12481 gtatccggtt cggatagcgg taaagccggt cgcgcttctt tcaactgcac atgctgaatg
    12541 acaattccag ttttataaag atcaaatttt tcttgcatat aatttttagc tcgctcggcg
    12601 attgctcgtc gttcagtctg gaatacttcg cgaaaggaaa agtcaccaac tgccgaacgc
    12661 atagccgccg cgccgactaa tcgtaacata ttctccggat tttcaactcc aaataaatag
    12721 gctaccgggt cttgaatgct gtactgaacc acccattgca aatcaaccat tttcaagtca
    12781 ccggttagca aacgcgcttc cggtagatag aaattatccg acagagcagt cagactatcg
    12841 gaggtagtcc gagtaccaaa ttcagcagtg taagggtaat taacttttac ccgatatagg
    12901 cgatcaatgc cccaaggtaa tttccaatga gcaccaggtc cattgatctg gtgaactttc
    12961 ccgcagcgag ttactacacc ggtttcgccg ggctcaattg tataaaatga catcaaagcc
    13021 gtaacaatta ctaccaacac taacgcaatc agaattatca accagtagga aacactcgaa
    13081 acctcgattt ctttattact gaattgaact tttttaccag ccatttttcc tcctgataat
    13141 ttcataagaa aaacatcaaa ccacgcgtcg taggcgagca accggaattt tcatttgctc
    13201 gcggtattta gcgactgtgc gacgggcaac taaataaccg ttttgcttga gtaaattgga
    13261 taattgctca tcactataag gtttatgctt gtcttcatta tcaataattt ccttcagctt
    13321 ttccttgact atccgagtgg aaatttcctc accgtcttcg tctttaatac cttcgttgaa
    13381 aaagtatttt aactcataaa tgcctgaggg caactggaca tatttattat tagtaactcg
    13441 gctgatagtt gagatatcca tattgacatc atcggcaata tcttttagaa tcatcggctt
    13501 cagcggttta ctgggatctt cgaagaaatc caattgtcgg gcaatgatgg ctttcataac
    13561 ccgaatcata gtcaggcgtc tctgttgaat tgcctgaata aaccacttgg cgttatcaat
    13621 tttgcgcttc aaataattct tggtctcatt gtcaatttta gaaccattgc taatcatctg
    13681 caaataggtg gaactaattt gcaattccgg gatgcgcgta tcattgactt cgacaatata
    13741 ctctctatct actttttcga cgatcaaatc aggaacgata tattgcggat cgagcgtgtt
    13801 caaagttgca cccggtttgg gattgagttt ggttatcatc tcctgaacgg cttgcattcg
    13861 ttccttgtcc cagccgtagg ctttcataat attttgatag cgatgattgg caaaatcctc
    13921 gaaatgctca ctaatgactt tgatggcatc atctgaattc ggtatttgat taagctggat
    13981 caaaaggcac tctttcaaat cgcgagcggc taaaccgggc ggatcaagtt cctggatttc
    14041 tttcagcact ttcagcactt ttttctgggg caatcccagt ccgaacgcga tattttctat
    14101 cggcagggtg aggtaaccgt tctcatcaag attccaggct atttcagccg caatccgttc
    14161 ttcctcatca tcaagattat gtaatttcaa ctgatcaaaa atgtgatcaa ccaatccttt
    14221 cggtgccggt tgaataaatt gtgtcttttt ctggtcggaa tctctttctt ctttgggtgt
    14281 ataatcgtcg ttttgcggaa gaatttcctc ccaatcaatg tcatcttctt gggaagtttc
    14341 cggattatcc tgcaattctt cttcggaact ttcactaatc tcaggattga cttcttctaa
    14401 aaccggattc tgttcaagtt cctgaagcac acgagcttcg agagccaagg tattcaattg
    14461 caaaatcgtg gattgtagaa tttgctgggg cgtgagcctc aattcctgag aaagtttctg
    14521 aacctgggat tgacttaact gcatcatctg gcgagcttga atgcttctcc taaatataag
    14581 cggcgggcat cttcatcgtt agccaaaaat tcggacgtac ccgatttaag aatacgacct
    14641 tcaaacatta agtaagaacg attagtaatc gaaagagtct cgtgcacgtt gtgatcagtt
    14701 atcaatacgc caatatctcg atcttttaag ctgacaacga ttttctgaat atcttctaca
    14761 gcaatcggat caacgccgac aaatggttcg tccagcagta taaaatccgg gttggtaatc
    14821 aaggcgcggg taatttccgt gcggcgtcgt tcgccaccgg agagggtgtc cgcccgtctt
    14881 ttgcgcaact tgcgaagctc gagctggtcg agcaggattt cgagtcgctc cttctgctcc
    14941 tttcggctaa tgcccagtgt ttgaagtacc agcatcaaat tttgctcaac ggttaattta
    15001 cggaaaaccg atggctcttg cgagagataa ccaatcccta gttgggcgcg tttgtacatc
    15061 ggcaagcgcg tgatttcctg ttcattaatg aatacttttc ccgaagtcgg cttgatcata
    15121 ccggtaaaca tatgaaatgt agtcgttttc ccggcaccgt tcggtccgag caagccgacc
    15181 acttcgccct tggacacttc gatattgacg gaatcaacga cccggcgttt gccgtatatt
    15241 tttaccaggt tttcacctcg tagagttttt ttttgcggtt ggctatttaa ttccatattt
    15301 tagtatttca actcccggct ggttactcca gaaggtttga aaatcaccca atttttcaaa
    15361 tccgattcgg attcaaatcc aattccggtt agcgaatctt cgggagtcac aaaggttaca
    15421 aaaacttctg aaataattcg ctcttttcgg tgatcccagc gtaattcctc ggtgaaaagg
    15481 gtaaagccac tatcagaaac aactacaaca ttacctttgg caattagatc gttcgtgtgt
    15541 tcattaacag tcgctgtctg agaagtcaaa tgcgataaat gacatccatc cctatcatag
    15601 aaatctactg ccacatcggt atccatatat atcaacgcct gatcattatt cttcgtcaaa
    15661 tggccagaat gaaccaccgc tcgtttttgg ccgtctttag tcagggtaat attcgaattc
    15721 cagatttctt gatccgggta ttgccggtca atcatggtag gctcattact cttatcttct
    15781 gcgcaacccc agcaaaatag gacagcaaat aaggttgata ttaaaaatct tcccttcaag
    15841 gcataatccc gtagcgcaaa atatcatgaa tatggataat tccgatcggt ttgcgatttt
    15901 gttcgccatt atatacgaaa aggttggtga tcgaatgttc ttccattaga tgcaaggctt
    15961 caatcgctaa actacttttt ttaatccatt tcggattatg ggacataact tttagagcag
    16021 tttgttccat aaaatccttc tgcctttcga gaccgcggcg caagtcaccg tcagtgacaa
    16081 caccaatcag gttttcggaa tcgtccagga cgccgacaat gccgaggctt ttttttgtca
    16141 tcatataaag cacttcaggc attttgtcgc tggatttgac aaacggtatt tcttcaccgg
    16201 tgtgcatcaa atcggctact gtagtcaaga gtctgcgtcc cagtgaaccg ctgggatgta
    16261 aaccggcgaa atcgcgccga ttaaaatgac gtttcgcgag caaactaatc gccagcgcat
    16321 cgcccatagc taaggtcgca atcgcactgg tagtcggcac caattgcaat ggatcggctt
    16381 cgccatttac ttgagtacta atgacatagt cacatttttt tgccagagtt gattcattac
    16441 gccccaataa gccaatgatg ggaatgcgca tttttttcag cggtggaatt aattgaatga
    16501 tttcgtcggt ttcaccgcta ttcgaaataa tcaaaagagt atcatcagga gaaattaccc
    16561 ccaggtcgcc gtgtcccgct tcataagcat gcataaaaaa actcggcgta ccggtactgg
    16621 caaaagtagc ggctattttg catccaatat gtccggattt gccgaccccg gttacgatta
    16681 ctttgccttt ggtgttgaat aaaacttcga ccacttcttt aaaattctgg tcaactttat
    16741 cgggtagggt agcaatagct tcggcttcgg aacggagtaa ttcccgggct ttttcgagat
    16801 atttttctga agttgattta tttttcggca taggtatccc ccgttaaaat tccaatcgct
    16861 tttgacttta agtgctgggc atttagcact aaatcaatca cttcgcgcac agcaccatat
    16921 ccgccgccta acacggtaat gtacctggca aattgcttga cttctggtaa ggcattggct
    16981 accgctactg gcaatccaac tcgcttcatc agaggaatat cggggaggtc gtcaccgata
    17041 taaatgattt cctgatccgc gacggcatac cttttcttaa tttctgcata gggttcgagc
    17101 ttggctagat ttccctgata aagcgcatcc cccagaccga gttcttgcat ccgcacttct
    17161 gtagccgcag atttccgccc cgaaataatt gccagcggaa agtttacagc tttcagcaac
    17221 gaaattcctg ctccgtctaa aacatgaaag cgtttgaatt cctgaccgtc agtgccgata
    17281 tatagcgccc catcggtgaa aactccatca acgtccgcaa taatcaagcg gattttcgaa
    17341 agaagttcat ttatgtcggt attcattgaa attttctcac taactcgccc agctctatca
    17401 attctttcaa taattttggt aaatcttcca gcggatattg agtcgcccgg tcgctcttgg
    17461 cttcggctac attatcgtgc acttccataa aaataccatc acatccagcc gctaccatag
    17521 cccggctgag atggggaata aattcgcgcg ctccgccggt aacattaccg ccgggaattt
    17581 gggcgctgtg ggtggcatcg aaaattaccg ggtagccggt ttcttgcata atcggcaccg
    17641 agcggacatc cgcaataagt ccgccatatc cgaaagaaat tcctcgctcg gtgagcaaaa
    17701 tcgaatgatt accggttgat tcaattttgc gcacaatatt gacaacatct tgaggtgcta
    17761 agaactgtcc ttttttcaca ttaattactt ttccggttcg acctgccgcc agtataagat
    17821 cagtctggcg acacaaaaaa gccgggattt gcaaaacatc cgcaacttta gcggcgattt
    17881 cggcctgaaa cggttcatga atatcagtca gaatcggtaa gccaaattgc tgtttcactt
    17941 ccgctaagat agccagacca gcttccaagc caggtccgcg aaagctctca atcgaactgc
    18001 ggttagcttt atcgaaagaa gatttataga tcagcgggaa atcaagttcc tccgagagtg
    18061 cttttagctt ttcagccatt ctcaaagcgt gatcgcggct ttcgattaca cagggtccaa
    18121 gaataaaagc caatggttga cggttgccaa tggcgatatt tttaactttg acttctggca
    18181 ttttccgctt tttggttatg atatttccgc gccgcgcgca caaattcacg aaaaatcggg
    18241 tgggctttat taatccgtga tttcatttcc ggatgaaatt gacaagcaac aaaccaggga
    18301 tgatttttca gctcaatcat ctcaaccaga ttcagttctt tattgaccgc accgacgacc
    18361 aaaccggctt tgaccatttc agccaaataa gtattattga cttcatagcg gtgacgatga
    18421 cgctcggaaa tttgcttatt tttataaatt tcataggcta aagtaccagg aacgacttcg
    18481 gcggcataag ccccgagccg cattgtaccg cctttattga aaacgcgctt ctgctcctcc
    18541 ataaggtcaa tgaccggatg ctgggtattt tcgttgaatt ccgtactatc ggcgtccttc
    18601 cagccgcata cattacgagc aaattcaact acagcacatt gtagcccaag acaaataccg
    18661 agaaacggaa tgccattttc gcgagctaac ttaatcgcat taatcttgcc tttgacaccg
    18721 cgttcgccaa aaccacccgg aaccagaatg ccatcaacat tttttagttt attgacagtt
    18781 tcggagtcat tggtcaagtc ttccgattca atccagacaa tattaacgcg cgtattattc
    18841 tccgcaccgg catgattgaa ggactcgata atagacttat aagcatcgtg caactgggta
    18901 tattttccgc aaatggcaat attgacctcg tcccgcgggt ttttgatcgc ctgaacgaac
    18961 tcctccaagt ctttaaggtc cggtttccgg cgtggcaaat tcaataggtc ggtaatttga
    19021 tcacccagcc tttgttgatc aaaaatcagt ggaatctcat aaattgtttc tgcatcaatg
    19081 gcatcaataa ccgcttccgg tacaacatta caaaaaagcg ctatcttttt gtgaatgctt
    19141 tcgtcgaggt gaaatttcga gcggcatagc aggatgtcag gttgaatacc aatctcgcgc
    19201 agacgcataa cactatgctg agtcggtttg gttttaattt ctccactggt ttcaatgtat
    19261 ggcacgagag tcaggtgaac attgatgcaa ttgctccgcc ccgattcaaa acgaaactgg
    19321 cgaatagctt ccagaaacgg ctgactttca atatcgccga ccgtgccgcc gatttcgcaa
    19381 atgacaacat cataagcaga attgacatta accgccgtta tgcggcgttt gatttcatcc
    19441 gtgatatgcg gaatgacctg aaccgtcgct cccagataat caccgcgccg ttcgcgatta
    19501 attacttcat agtaaatttt tcctgaggta ttgctattgc gcgagttcat attttcatca
    19561 atgaagcgtt cgtaatgccc cagatcgaga tcggtttcag aaccatcttc cagaacgaaa
    19621 acctcgccat gctggaatgg gctcatcgtt ccgggatcaa tgttaatata cggatcaaat
    19681 ttctgaatgg ttaccttaaa accacgtgat ttcaggaggt atccgataga ggcacaggca
    19741 atgcccttgc cgagaccgga gataacacct ccggtaacaa aaatgtactt ggtagttttg
    19801 tcagttttca tattcttcca gatatttttg ggctttttta aaatcatcag gagtatcaat
    19861 actcagtgat ttctgatttg ttcgtacaac tttaatgcga taaccattct cgagaatgcg
    19921 taattgttca agcttttcta atagttccag aattgtcacc gctaggctgg taaatttttg
    19981 caaaaaatcg gcgcggtaaa catacaatcc aatatgcacc agcgccgggt gatttattaa
    20041 cgggattgat ttatcccgga tgtaaggaat atttgcccga ctaaagtaga tggcttcgtc
    20101 tcgtttatta accagtactt taacaacatt ggaatcggat agatcagctt gcgttatgtt
    20161 atggcacgcg gcggttgcaa ctggcagagt cggatcgtca atcaagcttt gcacagttcg
    20221 gtcaataatt tcagccggta gaaaaggttc atcaccttga atattgacga caatatcagc
    20281 ttcgttatcc cgcgccacaa gagcacaacg gtcggtaccg gaagccaatt caaccggagt
    20341 cattcggccc tccccgccga acgatttaac gtgttcaaaa acccgctgat cgtcggtggc
    20401 tacgattaat tgattaatca attgagaatt acgggcattt tcatatactc tctggataag
    20461 cgtttttccg tggaggctaa ccagcggctt gccgggcaga cgggtagctg caaaacgtgc
    20521 gggaatgacc cccagcactt tcatgggtta atgtccttga tgactttagg aacaacgaag
    20581 tatccggctt tttcttgtgg ggcattagca aaaaccgcct ctttgggtag agacggtttg
    20641 acttcatcat ttcgaaaaac gttagttatt tcttggacat gagatagcgg cttgacatta
    20701 cttgtatcca gattttgcaa ctgctcaatg tactctaaaa tctgatccat ctgagcggca
    20761 tatcgctcga gttccgcctc gctcaaggtc aagcgtgcca aattagctat tttctccgcc
    20821 gtttttagat caactcgcat ttttgcagcc catacttcta atttcgccga gcaatttaaa
    20881 gattttacct gcttaaatca agcactggaa ttctgtttgg cttagttttt gttcgccgat
    20941 gaaaatttta ctacatacgt tccggtgcgg aaattcccag taacgccaat ccattagcca
    21001 taacgatttg gacggctttg accagcgcca ggcgcgcctg cgacaattcc caatcttttg
    21061 aaatgacgcg atgaactgta taaaatttat ggaaagcagt ggcaactttt tgtaaataag
    21121 ttgttagatg ttggggctcg agagtgctaa cacaaattgc aataacttct ttgaattcgc
    21181 tgagcgcatt gagcaagctt aaagtctctt cttccttcaa taaagtcata tcgaagtcat
    21241 ttcctacggc aagatttttc gtagccgcat tccgcaaaat gctgcaaatt cgggcatggg
    21301 catattgcaa ataaaaaacc ggattttctt cactctcggt ttttgccaaa tcaagatcaa
    21361 aattcaaatg gctcttcata ccgcgcatta ggaaaaaata acgcgctaca tcccgcccaa
    21421 catcgtcaat caattcatca agcgtgacat agttcgcctt gcgggttgac atctttactt
    21481 tttcaccacc tttaattaac gtcacaaact gatgaatcag gactttaact cgcgaagtat
    21541 catagcctaa ggcgcgcaaa ccagccagaa catccggata agtagcgtga tgatcggctc
    21601 cgaaaatgtc aattatcaaa tcgtagccgc gttcaaattt agtgacgtga taggcgatat
    21661 ccggcaaacg ataggtagct tcatctacga cgcttttcca gagaacgcgg tcgtcggtgc
    21721 ctccataaag acccgcccga aaccagatgg cgccatcctt ttcataactg gcgccgatgg
    21781 ctttcatttt ttccagaacg gcgttcacct gcccattttc atataggctt ttctcattat
    21841 aaaaattgtc aaaaaccacg ccaagtcgtt ccagagtttg ctttatatcg gcgaagattc
    21901 tctcttcggc tgtaattcta aaagcctcgt gatcaggatc gtcctgtaag ctatcgccat
    21961 acttgtttaa cacttcccga gcaatttcta tgatatattc gccttgataa tggtcctcgg
    22021 gaaattcgcc cttccaaccc aacaattggc gataacgcca ataaaccgaa tgcccgagca
    22081 tacgcatttg ccgcccggcg tcgttataat aatactcgcg atcaactcgg taaccaaccc
    22141 attccaaaat acgcgctacg gtatcgccta aaaccgcctg acgaccatga ccgacggtca
    22201 gtggaccagt cggattggcg ctgacaaatt cgacttgtac gcgctgattt ttcccaatat
    22261 cgagtttgcc ataattttca gcggctaaaa tgatttgggc aaccgtttgc tgaagcacac
    22321 gcggactaag gaaaaaattt ataaatccgg gcgccctgta ttccactcgt tctatatact
    22381 cggattgggg aagcgttgca actaattttt cagcaatttc aagcggagat tgacgctgga
    22441 ttttagccac cgctaaagcc agatttgtgg tataatgtcc gaaagtttcc tgttttggtc
    22501 gctcaatctg aatcggttcg cggtaatcaa tgtcgttttg ctgaagagct ttttccagca
    22561 aaccgcggat atgttctatc atttcattcc tttaaaaaaa tcggtctaaa tttaagcgcc
    22621 cgtctattcc tgtgcaaacg tcattaccgt ataaatttaa tttacttgcg gagcgtcagt
    22681 agattgcgtc gccaattagc aaagccaatg cgacggatcg ggctatcccg gaagatttca
    22741 ccgaattcat cttctgtaac tttcagccaa tcagttagtg acttgctgaa ctccgagtaa
    22801 tattcagaat atcgagtatc tcctggccgc ttccattttt gattatacgg acaaattttc
    22861 tggcaagtat cacagccata aatccagccg cgtaaattct tcttttcagc cgccgaaaat
    22921 tcgtcgtctt tctcaatagt cagatatgcc gtgcaaagcc gagcatccaa taaatgctca
    22981 tctaccaggg cttgattggg gcaagcatcc cgacaaagtt gacaatcgcc acaaagattc
    23041 gactgcggtt cgttaaaatg gagctcaaga ttcagaaaaa tcattccgat aaaacaatac
    23101 gaaccgagat ccgggacgat cagcaaattg ttttttccga cccagcctaa accggcttgc
    23161 caggcgaaga ttttttgcat tactggaccc gaatcaacgt aaatccggga tacagtcgcc
    23221 ggctccaata aattaatttc gcgactaact ttcgccaatt tttgtttaac aactttatga
    23281 taatctttgc cccaagcata acgcgcaatt ttgggaatcc cggttggcca ctctacattt
    23341 tgaaaataat tatccgctac caccagaacc gactttgccc agctgaagta cgttttctga
    23401 taattctgaa aatcgtaata tttccgcatc cagctcatcg tgcctaaaaa tcccgcatca
    23461 atccattgct ggaaaaaagt gatatattta tagtctaaaa aaggcggaga aattcccaca
    23521 cgcgccaatc cggaattctc agccagacga ataatttcag ccgtcagact catcaatatt
    23581 ttttttaata aaaatttaaa aacattgccg attatactcg acgatcaatc ggcagaatca
    23641 tcactaatta tttccgtggc ggcatctgcc cagagtcttt caagattata atacgcacgc
    23701 gcctcaggat tgaaaacgtg caccacgaca ttgacatagt ccatcaaaac ccagctgagg
    23761 tgctggtaac cttcgatgtg ccagggcttt tcagccggtc gcagacgatc atcaatttca
    23821 tcaacgatgg ctttgacgtg ctgaccaacc gcgccggtac agatcacgaa ataatccgtt
    23881 atcgaagtca acggtcgcaa atctattatg acaacatcac gcgctttctt atcaactgcg
    23941 atgtccgcaa tttgccgggc taaagggtag gattgatttt tatctgtagt attctgagag
    24001 gttttcaaaa attaaaacgt ctctccgata gctattattt tagaataaac tggcaagcgt
    24061 tggtaatctt tgccgacaat caaagtcagg tcaacctgga gggttgagtc tattttctga
    24121 aacaattgat cggaatcaag cagtaaagtt ccgttcactt cccgcaattt ggtaacatcc
    24181 gccgtacggg ttatcagcat agttttttcg taaccagaat aatcagcgtt accggttgaa
    24241 ataacatcat agccattttg ccgtagaaag ttggtgaatt tttgagctaa accgggtaca
    24301 tcagttccat ttaagacttc aataacaatg tacggtaccg gtctttcatc ggggggaatt
    24361 acattcacct gcgacatagt taatttttca tttggattgt taaagcgttt tcccatcgag
    24421 aagataaaac caacaataac taccgaaata gaaaaaattg ccagattgag aaaccatttt
    24481 tggaggtcaa ctgcccggcg tttgttttta cgagaggtag ggcgtttgcg acgattacga
    24541 ctacgtgcca ctcttaatat cccaagtaag gattgtaata ctcgtatgga taataatacg
    24601 gtctgcggtc aaaacgaatc ataaaggtgg cgttttcctt gggacgatat attaggtcag
    24661 ctccaaatat taaattatcg agcatattat ttgaggtagc gttaaacgtc cctaattgca
    24721 gcggattgtg ataaaatcca aagcgggtgt ttagtaaaaa tttgttcgag agcaaaaagg
    24781 acatcgcatt ttggtaaact cctaccgaca tcgattgatt accaaaggtc gaataggata
    24841 aagaataaga ctgttgcatt ttaaaccggc tgggatcaaa taggctataa ttggatattg
    24901 cggatgaaat accgggcaaa tgaattgctt cgccgattgt aggtgacctt tgaatctgag
    24961 tcttcagctg agcaaacccg ctcgttccga gaaaaatcgc caacatcgtt actgttaaaa
    25021 aatttctttt catcttaaca ccgccattga catttttcat actacatttt aaatctttta
    25081 cgggattttg tcaagaccaa actcacactt ctttaaaatt atctttcttt caaataaata
    25141 ttatctatca aacgcgtctt gccataatac acggctaccg cggcgaaagt tcccggcttt
    25201 accgtttcaa ccggcgtcag cgtttgatca tcaataattt cgacataatc aatccgtgcc
    25261 agtggcgaag tctgaatcat tttagtaatt tggtcacgga cctggttggc gtcggtcaga
    25321 ccattcttga cattttctcg ggcggcttgc aaagctttga aaataatcgg agcttgttgc
    25381 ctttcctccg gagtgagata aacatttctt gaactcattg ccagtccgtc cgattcacga
    25441 actataggcg ccactataat ttggatcgga aaatttaaat cccggaccat ctgttttaca
    25501 ataaccgctt gctgggcatc tttctgacca aatacagcca catcaggctg gatgatattg
    25561 aacagcttgg caacaactgt tgtgactccc cgaaaatggg tagggcgact gattccacac
    25621 aggacttttg ccaattgctc ggtcacaaca taagtaaaat aaggatcggg gtacatttca
    25681 gccacttccg gatgaaaaat ataatccact ccgcgttccc gggctaagcg ttcatcacgc
    25741 tcgatatcgc gcggataacg catcaggtct tcgttcgggg caaattgagt cggattgaca
    25801 aaaatgctca taacgaccac atcggcattg actttagcga catctactaa tgagagatgc
    25861 ccctcatgta agtagcccat cgttggtaca agcgcgattt tcttcgcgtt ggttcgaatt
    25921 actaataatt cctgacgaat atcttcgatt ttagtcaata tctgcatatt ttatttaatt
    25981 ttgagctgct tcaataactt tcagattcgt ccggaaaatt gccgttgcgt acatcgttac
    26041 caaaggctgt cgtcgctcgg cgaatttcat ccgccagatt agcgtatcgg cgcacgaatt
    26101 tatatttaac ctgatcgaat aatccgagca tatcataaga caccaggacc tgcccatcgc
    26161 attcttttcc ggcgccaata ccaatcgtgg gaattcttag ctgagcggta acatctgccg
    26221 ccagtttggc tggaattttc tccagcacaa tggcaaatgc cccggcgtcc tgtaaggcaa
    26281 gcgctgattt ttcaatatat ttggcttctt cagtttcttt acctcgcaca ccaaatccac
    26341 cgaatttatg gacactttgc ggagtaaaac caagatgtcc cataaccgga attccgattt
    26401 cgaccatttt ttcaacaaca ggacaaatcg gttcaccacc ttcgagtttt actgcttctg
    26461 cgccgcattc tttcataaag cgcccagcgt tatggagtgc ctgttcgaca ctttcctgat
    26521 aggacataaa cggcatatcg gctaccagca aagcccgctt gactccgcgt cgcacacatt
    26581 gcacgtgata gaccatttca tccattgtca ccggtaaagt agtttcgtat ccggcgcaga
    26641 cattcccgca ggaatcgccg accaggataa tatcaacacc cgattcgtcc attaagtgcg
    26701 ccatggtaaa atcataagcc gttaaggatg tgattttcac gcctttttgt ttcatttcga
    26761 gaatagttga gactcgtact ttatctttca tttcctcacc atttcttgct taaaatttaa
    26821 atatttttcc caatatgtcg gacattgatc atcgaattta ttggatgatt ccgcccatca
    26881 gaaggcaatc atcctgataa actacagccg attggccggg acaaatcgct ctttgcggtt
    26941 caataaaagt aatttcatat tcgttatcat taatcctttt tccgtgaccc atagcgcccg
    27001 gatgattata acgtatttta attatgccgt cgaattcagc tacggcggat attccgaccc
    27061 agttgacatt ttttattacc attttcgaag agaaaagcga tttatcaggc gcaatcacaa
    27121 ctcgattttt ctgggcatta atggctttta cataacgccg ctccttgaaa ccgacgcgga
    27181 agcctttgcg ctgaccaatt gtaaaattgt aaaaaccatt atgccagcct aaaactcggc
    27241 cgcgttcgtc caccaattcg ccccgtccaa tggtttgcac tttctggggc gcgtattcct
    27301 tcaagaaact acgatagtcg ccttcgggga taaaacaaat attctggctt tcggtgattt
    27361 taccgacgat aagattatga tcaacagcaa ttgttttcac tgcattttta gtcatttcgc
    27421 ccagcggaaa aagggtgcgt ttcagtgcgg tttgcggaat ttgccagaga gcatacgatt
    27481 gatccttggt tgaatccact cctttccaaa tctggtagcg tttgtgtgct gaactatatt
    27541 taagacgggc ataatgaccg gtggcgattt tttcgattcc atactggtcg gcaattttca
    27601 gcagattatg ccacttaata acacgattgc aattcacaca tggatttgga gtcaatcctt
    27661 gaaaatattc attgacgaat ggtttaacta cttgctcttc aaattctgca tttaaatcga
    27721 aaattatatg cgggattttc aatatttcac aaacttgccg agcgcgatca atggcttttg
    27781 cctgggcaat atcttctttc attcgccaca tccgaaaagt gattcctacc ggttcatagc
    27841 cttcttgaca taacacccac gccgccactg cgctgtcaac gccgccactc agagctacta
    27901 atactttttg ctttttcgta accaaggtta ttttatcatt cgccaatttt caattaattt
    27961 ttcatgaaag ggaaaaacca gagatggatg atcgtctatt ttaaaccagg cggcttccgc
    28021 cgcatcactg ccaggacgaa ttaaatcttc ttggaccggt gtagcataac caatcattaa
    28081 aatatcacca tagaatccat tcaagtgagt gccaacatca accaatttta actccgtagc
    28141 gcttaaaccg gtttcttcgc aaagttcacg aacagcggct tgcgccgcgg tttcacctat
    28201 ttcgatgaat ccacctggga ggctccagcc gccccgtcct ggttcgactg cacgacgaat
    28261 taaaacaatt tcattttgtt cgttataagc aattataacg acagtgggta gaggattttc
    28321 atatagaatg agctgacatt gaggacaaaa tgcgcgtcgg tagccttcca tttcgagcga
    28381 agttgttggt gtaccgcaat gataacaata atgatacttc acaagctcaa aaatagacag
    28441 ttttcgggcg aataacaacc aattttatta cttgctaata aactctttga actaattgag
    28501 gttatagatt gtacttcggg gaaaatgcg
//
LOCUS       DAOSMP010000029        27460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_1751_length_27460_cov_15.987630, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000029 DAOSMP010000000
VERSION     DAOSMP010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 27460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27460
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1751_length_27460_cov_15.987630"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            564..1436
                     /locus_tag="PKX09_06285"
     CDS             564..1436
                     /locus_tag="PKX09_06285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013468229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOU17467.1"
                     /translation="MSVNYKIFQKDCLEWLHEINKSDKSYIHLTFIDPPFNQGKEYRY
                     FNDAQPDEQYWNWIRDILAQVYQITVEGGAIYFMQREKNTEQVLRILRETGWVFQNLI
                     IWRKKTSAVPSDIRFSKQYQIIAFATKGEKARVFNKLRIDLPIPPEYKYERANGIYLP
                     DIWDDIRELTSGYFAGDEAIRDVKGNRIHTQQSPVALLLRIILSSTMPGDTVLDPFAG
                     SGTTLVVAHQIERNSIGIEIDPEYIKIIEERLKRLRSADNVLAYYKYYRYSPNLEKIW
                     SSEKPVITEQMRLL"
     gene            1437..2018
                     /locus_tag="PKX09_06290"
     CDS             1437..2018
                     /locus_tag="PKX09_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17468.1"
                     /translation="MNEFLDAVELFIGVVNGLKKKQINLSSSLDEIVLKACGTNLAYV
                     DNRRSAKEEFNFDFWDSIKKQFKDLKDFIDWIKTYGIDERFLYSKSEQFPDFLLKVKK
                     HDNKLICGSLLELKDSKGGSIASFNSTLPTKYKSLDEVDIIIGHKRIINPFGPINGAT
                     LLYANHDGHPEAIDPYYLAAQTCIRERLNDQRD"
     gene            2015..2230
                     /locus_tag="PKX09_06295"
     CDS             2015..2230
                     /locus_tag="PKX09_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOU17469.1"
                     /translation="MKGIIMEFKIDKTKLKIAMLQHGILTIDELAAKLGCTRQNFYQT
                     YIKDSALQKNIPKLCEVLGVPPEDILK"
     gene            2242..2814
                     /locus_tag="PKX09_06300"
     CDS             2242..2814
                     /locus_tag="PKX09_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17470.1"
                     /translation="MSNSTFKIQHSKFYLLILFFIVSITIGFSIGYLIFKNPKQSQPS
                     IYNFSAESNISKFLLKHKKLSPDKVTFINKKIYEIDEYFIIFEGKIRNITKSGIHNVI
                     IFFAILNYSDSDFYSMNMWWEDIKYHPSFYYQYFNYLPPNQDIDFKIPISLIGHFGDD
                     IAGPYTAIVHNNIYVDIIQASDLSEFPMIY"
     gene            3044..3571
                     /locus_tag="PKX09_06305"
     CDS             3044..3571
                     /locus_tag="PKX09_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17471.1"
                     /translation="MDGKLASDQNYHTFQRRNFYFIRTHKDDDDKVRISIVDGSFFET
                     VSKEHLINQMLLNILQGHLEKKGINISPEILNEVEKSISYVTDQTIIAASQNIEKASI
                     RPRLRIMAEVHPEGNPHSSFYPEISERSVNLIIQSSPYKEKIIKTISKKIPDVNVFSI
                     KHKRNGDHTVFQIKF"
     gene            3928..5151
                     /gene="hemW"
                     /locus_tag="PKX09_06310"
     CDS             3928..5151
                     /gene="hemW"
                     /locus_tag="PKX09_06310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOU17472.1"
                     /translation="MIDAKKVIKEKISTNFGSDSEKLGLYLHFPFCLQKCRYCDFASE
                     DQRLERIPAYTEALVKEIALRADQQAERAELQTIYFGGGTPNLFGINNFGKVTQAIRE
                     YYLTKNVAEFTVEINPGVLTAEFMESLKAEGVNRLSLGAQSYNDDELLTLGRIHKSRE
                     IDVAIERLRQTGFSNFSLDFIYGIPGQTLSSWQKTVEEALRTGAQHLSLYCLSYERGT
                     PLYQAQMNGEIQVIDEELEWQMFQTAHNLLVNAGFRHYEISNFAKPGFEAQHNSIYWK
                     GGRYFGFGAAAHSFDGEKRWWNERLIDDYIKALQNNCLPVAGQEKLSLSDRITEAVFL
                     GLRTAEGLDVALLEKLSGIEFAYIYNKLINKIGNNPEQFFSLQNGHLILTTRGWFLCD
                     SIIEKILTIIEEMKK"
     gene            5148..5486
                     /locus_tag="PKX09_06315"
     CDS             5148..5486
                     /locus_tag="PKX09_06315"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU17473.1"
                     /translation="MIIRKVKEVPAEPVAEPGAENVRRQVLLSPSEGAPHFTLRRFEI
                     APGGQTMYHHHNYEHEIYVLAGVGKARGRNSEVRINQDDAILVLPNEEHQIVNTGITP
                     LIFLCLIPNQ"
     gene            complement(5559..6575)
                     /locus_tag="PKX09_06320"
     CDS             complement(5559..6575)
                     /locus_tag="PKX09_06320"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU17474.1"
                     /translation="MKRILPIILVGFLVFSCAQKEKSAKLEKGTELYQLAQELSQQIP
                     ILDPEKNTVIVTTKKFDITVGDVFTTLHQLYGDGIQQLKSLDTNQLNEIIKGNAIALA
                     EKKLLLQAAEKNGIKISDAEVDSILELQYMQVGGKEKFLNYLSNANINFKALREDIRD
                     NLAINSYLDKMIMDSVNITEEDIQKYYNETDQTATVRHILKITQGKSPEEKAQIHSEM
                     EGILDRAQKGENFESLAKKYSDDPGSKDNGGLYKDFGRGEMVKPFEDAAFNLPIGSIS
                     DIVESPYGYHIIKVIDRKKESRPLDTVRSEIENILINSRQNELLINLLDNLKKENDFK
                     VVIP"
     gene            6712..6784
                     /locus_tag="PKX09_06325"
     tRNA            6712..6784
                     /locus_tag="PKX09_06325"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6746..6748,aa:Arg,seq:ccg)
     gene            6946..7911
                     /locus_tag="PKX09_06330"
     CDS             6946..7911
                     /locus_tag="PKX09_06330"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOU17475.1"
                     /translation="MVLLLRFQLFSQQFELRDMAEMQWYKGSLHTHAREGESDSPVEY
                     VVKWYREHDYNFLVITDHSTVTIPPAYAIEGDPSFLFIPGEEIMGYGTGRLLEINAFN
                     ITKDISPIHSETDLDALQKCIDTVRRMGGIPMINHPNYQWRLDADIILNSKDCNLFEL
                     YNGCPGTNNYGDDNHPSTEQIWDFMLSAGKRIYATATDDAHVYQKFSAELSNPGRGWV
                     VVKAKKLEVNEILQNLEAGLFYSSTGVEISDLIVKPVQIEIFIQMSGKNQYTTDFIGT
                     EGKLLLRTQNNPAVYKLTSNEKYVRAKIVASDGSCAWIQPIFVVP"
     gene            8137..8976
                     /locus_tag="PKX09_06335"
     CDS             8137..8976
                     /locus_tag="PKX09_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOU17476.1"
                     /translation="MLKEDLIYRGKSKDVVEIKSGPYTGKYRLIFTDRATGYLENGKP
                     VFDPGYDIVVGDIPGKGAIACRFATHFFKLLKDKGIPSHYIDTVSDNEMIVEPAIPLS
                     MPAESPEFPGAAPLLNLEFTWRNNATGSFWRRYPFVRPCRNIHTIVEAWTKGDSDILI
                     TFEALAETGAMTKDEIKQSIKLVKEIAEIVAQEFASNNLHVIDGKFELGRLKYGDGKI
                     VIIDEISPDVLRVCKGYSPDANGNCTVYKDCAMTSYADGKRTIKNKNQVQAADFVNIF
                     LSH"
     gene            9156..9641
                     /locus_tag="PKX09_06340"
     CDS             9156..9641
                     /locus_tag="PKX09_06340"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOU17477.1"
                     /translation="MEKCGIKIINMEVDLSKSERARKSKFYRIFPHTADLGVEVYGAN
                     LLELFEHSALALFDLMTNVANVMEQETCEFSVEAADRELLLREWLGELLYYSVSRQFL
                     FKSCHIKQFGEQTLIASASGEPFNPQKHILKREIKSVTYHELQIKNQGNHWKARFVLD
                     I"
     gene            9651..11096
                     /locus_tag="PKX09_06345"
     CDS             9651..11096
                     /locus_tag="PKX09_06345"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOU17478.1"
                     /translation="MEQLKINQISEQVWEIPPTGAMRVPGRIYANENLMQVIRDDQSL
                     KQVANVAQLPGIVGYSMAMPDIHWGYGFPIGGVAAFDIKEGVISPGGVGYDINCGVRL
                     IRTNLVADQLKIDIKNLIAAIFNSVPSGIGSHGAIRKLSRRELERVAVQGAKWAIEMG
                     YGSADDLQFIEDRGCLADAEPSVISQRAFERGADQLGTLGSGNHFIEIGKVVDIYDST
                     AAQAMGIFQGNLVLILHTGSRGFGYQICDDFIKSTLRATQKYEIKIPDRQLACAPLKS
                     IEGQDYLGAMRAAANFAFANRQVISSLIYDSLLKTLKISPNELGFRPVWDIAHNIAKI
                     EEHQYGGKKIHVCVHRKGATRAFGPGNPELPEIYQAIGQPVLIPGDMGTASYLCVGTE
                     IAMRETFGSACHGAGRILSRHQAKAKARSLDLYQALAESGVYVMAEEKGTIAEEMPFA
                     YKEVGEVVDSVDAAGIVRKVARFKPLGVIKG"
     gene            11261..13213
                     /locus_tag="PKX09_06350"
     CDS             11261..13213
                     /locus_tag="PKX09_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU17479.1"
                     /translation="MLKLRISIFTASLFLVGVTLAQDSDLDKWLELDLSELMNIKVTT
                     ATKRSQLIDKVPATVRIITDKEIQERAYHNLEDVLKDLPGFQFRDILGFNSYSFQRGA
                     PSQNNLILVMIDGIEINELNSGGFYGGYHYNLQNIKQIEVVYGPSSALYGTNAISGII
                     NLITKDAEDGPRAEASLTAGSFGTLFGDISLRLKPENLPLSVTFSTFYKQTEKADLGG
                     TKGDNNWSEDMENFENDYGFDSKIKYKNTILGLTLQDKQASRTTNYKSLGTNYLDYGT
                     NFHIRFLNAYLSNLYTKKPNWSLKSQIYYRNSTVMDNTIAYITTDTGQVGYYRPNSLI
                     GLEEKFDYEWSSKANLVAGVVMEAEKLASDFSITYSDDPEKAPAKPEEPDYENNTLIS
                     FYVQTQYKFWELVEMTAGARLDQSSYYGTILTPRLGFVYSHKNYTGKLLYTEAFRAPR
                     PWDYNWGIGNPDLKPERMKSIELANNLNFNQRLRCDFSLFHNQIYDMLIEKDSCWVNA
                     ERINTNGLEFNLDYRHEKIQLNLNYTFTDSKFSDGKQVPEIARHGFNAGVTYSLLANL
                     NVNLRGNYLSRRKNPVFISTTKSHYVDPYFVLNSVINYNPIPNLKLQIAVNNLLNARY
                     YHTSNRPPERYRQPQRAFLFKLGYQL"
     gene            13257..13799
                     /locus_tag="PKX09_06355"
     CDS             13257..13799
                     /locus_tag="PKX09_06355"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HOU17480.1"
                     /translation="MPYKKRIIVSIVCFLILSFSFIYANLPFNSETEINDLKAAFIYN
                     FTKYITWPDLERNKVFFIGVIGNSDIVNSLRQVAEKKFVKNIPIKVAHYQSVDDLRYC
                     HILFISASEKNQLDPILAKLKKRKTLTIGDTPGFCESGVMINFFMQEDRLKFEMNLAK
                     LESVGLKASSQIKKLARIVE"
     gene            13852..18468
                     /locus_tag="PKX09_06360"
     CDS             13852..18468
                     /locus_tag="PKX09_06360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU17481.1"
                     /translation="MKSINRGIFQKMSFRKKLIVIQLITVSTVIVFFIIFQVIINQIE
                     YQRDVNEKLETTARIIGSNSIPAILFLDNETAENILASLGSESEVVNAWLFDHNRNLF
                     ATFARDGFENYDYPFFDSGRYKLKSRYLIYSSELTQEEVAVGYILIRYQMHPLSSIIL
                     ESLGLGIVVLLIGFGLALVLSFYTGKTVSNPIMALVDTIKEISTRHDYSIRLPKTTQD
                     EIGILYDGFNEMLEQIQKKQAERDNAEKKLREANTIINRSPVVAFTWYNKPTWPVEFV
                     SESVINLFGYTAEEFIQGKISYESCIYPEDLPRVNSEVIKYSQTPDVQSFKHEPYRIV
                     TKSGAIKWIDDLSFIIRSESGEITHYQGIVSDITERMEFEELLRQSEIRYRRISSVVS
                     DYVFSAKINQDGVLVTDWVGGAFETITGFTFKEFSEAGGWRARLHPDDLEKDDHDFEK
                     LKKNEPIQSELRFYTKSGELLWLRVYAYPVWDYKNNRLIGVHGAVQNINERKKAEQAL
                     QESEVRYRLLFESNPAPIIIYEHNTYKILAVNEAFLQFYGYSKDEILAMRLTDLYPAD
                     EKKKIVKMLNDLYDYQDAVEWHHFKKDGTMVTVIFRSNDLKYQGHDARVAVITDVTER
                     KLAEEKIKNLNIELEKRVTERTADLVKEIEEREKIALTLEQSRESLRTIIESIPFPVV
                     LVNQDRTIRDANQATLDLLGFNSFDEIVGKNCYEILCIYNSDTCPILSPDQIVDKKET
                     VIINKDKVNVPVLKSAVPIKIEGEEVLLETYVDILKIKVMEMELIQAKEQAQAATQAK
                     SDFLANMSHEIRTPMNAIIGLTYLALQTELDARQHDYITKIKSSAQNLLEIINDILDF
                     SKIEARRLKLENIDFNLEKVFQDTANVVTFKAHQKNLEIIFGIDKNIPRYLVGDPLRL
                     HQILANLTNNAVKFTDTGEIIVRAEMDEEYDDTVKIKFSVKDTGIGLTKEQQDKLFQS
                     FTQADSSTTRRYGGTGLGLAISKQLTELMQGDIWVESVYGKGSTFYFTAVFKKQENQK
                     IEEFVLTPDLRGLKVLVCDDNKSSRLIIKGALETFGFKVVLASSGKAAIEILKKHKSK
                     PFQLIIMDWKMPEMDGIETIQTIRKDPEIPSVPAIIMVSAYSEEKIIDRIEKIGIDAF
                     LLKPVTNSTLFDTIMQVFGKTTPRRKRQADRGQRLKSELALIKGARILVAEDNEINQQ
                     VIDGLLTAYGMDVIVAENGRVAVQKCQEMHPSELDLVFMDLEMPVLDGYEATRAIRQI
                     AGYEKLPIVALTADAMSGTKGKVLSAMMNDYITKPVDPNEVYNILVKWIPAKRKKINK
                     GESEQQLAKNLNFPEVSGIDVQSGLRRVAGNQQLYGKILQNFVRENINLITSLQNYLV
                     DNDLEAVEHLVHSLKGSSGNIGAQKLYEVASAFEMELKSKSPDSKNIMELIQQLSMEL
                     ELVFNTIQKIELPAEIISSNKTNKPGKWGIEQEKKCQELKKYLAEYDARAGDIFYLIK
                     SELSQSIPEVDLTKIAESIEQYDYDQALSILEKLIELGDQINKD"
     gene            18470..19564
                     /locus_tag="PKX09_06365"
     CDS             18470..19564
                     /locus_tag="PKX09_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOU17482.1"
                     /translation="MENKKPTILIVDDVATNLNFLKGLLINDYDIKAANNGQLALKIA
                     RMGAGPDLILLDIMMPGMDGFQVCEELKRDPRTQDIPIIFLTARTATDDIVRGFKSGG
                     VDYLTKPFNPPELLARVRTHLLIKQQKDIILEQNREQKELLHILCHDLANHFTVISMS
                     LDLLKNDSAITDYLSKIKTATTHGIDIINLVREMRALQEKKIEISAVNLSECLAESVL
                     LLGDRFEQKNVKLLIDIDHSLWVMAERCSLINSVINNLMTNALKFSFEGGQVEISAEE
                     EDDAVIFKIQDHGIGMPAELMSQIFDITKITSRPGTKGEIGTGFGMPLVKSFVEFYGG
                     EIEVESRDIKEYADDHGTLITIKFINAKNN"
     gene            19577..>22044
                     /locus_tag="PKX09_06370"
     CDS             19577..>22044
                     /locus_tag="PKX09_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU17483.1"
                     /translation="MLKVLIVDDNNESRSLLESFLNRHDYQVFSAVNGKLALDILEEN
                     DVDLIISDILMPEMDGYMLCNIVKSDPRWKDIPFIIYSATFTSRQDEIFALKLGASRF
                     IRKPVESDLFLNIIQEVLQNRIEGSLEVAYPRFQDEKDAFKLYSERIIKKLEKNVSEL
                     EESEEHYRAIFENAAIGLYRSTPDGRILMANPTLVKMLGFDSFEELSQVNLEEGGFDL
                     KYPRSEFKKRIEAEGKVQNLESVWKRKDNSIIHVRESAIAVRDASGVTKYYEGTVEDI
                     TARKKAEEELRESEKHYRELIDGMIETVWIIDFNGDLMDVNRTATEVLGYTKEELLKI
                     GLFGIDSSLKREDIKTLARSMTKDKIQIFETTHKTKEGKSIPVEIYSSLIDYHGQPAI
                     LSIARDITERKRAEAALRESEERYRAVVENSQNAILIVGEDFKFIYVNDALCTLVGRS
                     RDEIIGHDFREFLDDESKALVGERYLKRQRGEDVPNRYEFNVIRSNGEIRRVEISSTV
                     VKDTQGNLLTIAQLMDMTERKRAEEDLVRIMESAQAILWHSPVVRQEGTEPEFLWKTR
                     YFNLDAIKNIIPLPDYPTHDINDRYYYSILEEDRRAMDRVSSAALKSGADRYSQEFRL
                     RDANGVIHWMYEDARIKKIDESHFEVVGVITEVTERKKAEIALKLSEERFKSLFEYNS
                     SAVVIYEVVDNGNDFIIRDLNKAGEKIDKIKRSEVVGKSLLEVFSGVKDFGLFEVLQR
                     VWRTGEPENFPVTFYKDNRISGWRDNYVFKLPSGEVVAVYDDLTKQKQAEEEVRKSEE
                     NFRLVWENSVDGMRLTDENGIVIMV"
     assembly_gap    22045..22144
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22145..23326
                     /locus_tag="PKX09_06375"
     CDS             <22145..23326
                     /locus_tag="PKX09_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU17484.1"
                     /translation="EQKMRQIEKEELQSQIVQLQKMEAVGNLAGGVAHDFNNLLTIIQ
                     GYTQMLMAQKDKTEPDYQGLKHVLDACDKAANLTRQLLLFSRKQPMEFKPVNINNTIT
                     NLLKMIKRLIGENIKIITNLKEPIQNIEADEGNIEQVIINLVVNARDAMPNGGTLTIS
                     TDNVALSAKECQTIKDSHPGRFVRVSVADTGQGIPSDLIEKIFEPFFTTKEAGKGTGL
                     GLSVVYGIIKKHNGWINVQSEIGAGTVFDIYLPATEITVDDKAHLSAETQMKQGNGEK
                     ILTIEDSADVLKFLAETLHQNGYTPITAANAAEAIEQFQNENGQIDLILADVVLPDKN
                     GVTVSEEILAQKEDIPVILYSGYAEESIIQLIKKNPKFVFIQKPFKMKELLDLIYQKL
                     H"
     assembly_gap    23370..23469
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(23604..24884)
                     /locus_tag="PKX09_06380"
     CDS             complement(23604..24884)
                     /locus_tag="PKX09_06380"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOU17485.1"
                     /translation="MKLLRKSFLIFITNLFVFITLNAQSLNKAVYKPRYHDPVLEQMK
                     EQREKDIAREDSLTAAIHTQQEARRKINKKDVLSLQSDLSGVKKPASLNVFKTIDHFP
                     PIAQYQSGMCWCFCGTSFLESEVMRLTGQKIKISELHTIYYEYLEKAARFIRERGDSF
                     IGEGGEVNGVFRIMKKYGAVPAEIYTGLPNGDKHDHTRLFAEIHNYLEYCKTNNYWDE
                     EIALNSIKLILNKYLGEPPQSFIYEGQEYTPVTFLNNVLKLQLDDYCDVMSTTAVPFY
                     IRAEFKVEDNWWHDSSYINIPLAEWYKFLKKAITGGYSLAIGGDVSEPGYLGFEDVAF
                     IPDFDIPFKYINQDSREFRIYNKTTTDDHGLHLVGYTRIDDRYWFLVKDSARSSRWGK
                     YEGYYFYREDYIKLKMLTYTVHKDVLKEILNQVK"
     gene            25053..25448
                     /locus_tag="PKX09_06385"
     CDS             25053..25448
                     /locus_tag="PKX09_06385"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOU17486.1"
                     /translation="MEMIIDFPKGLRVDAHLGKFTIRTDQPLSSGGEETAPTPFALFL
                     ASLGTCAGAYVLSFCRQRNLPTEKIRLVQRTERDTATGMISRIQLEIQVPEDFPEKYY
                     DALVRSANQCIVKKHLENPPHFEVFTKVV"
     gene            25520..25593
                     /locus_tag="PKX09_06390"
     tRNA            25520..25593
                     /locus_tag="PKX09_06390"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25554..25556,aa:Arg,seq:acg)
     gene            complement(25704..25781)
                     /locus_tag="PKX09_06395"
     tRNA            complement(25704..25781)
                     /locus_tag="PKX09_06395"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25744..25746),aa:Pro,seq:cgg)
     assembly_gap    25785..25884
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(25942..26421)
                     /locus_tag="PKX09_06400"
     CDS             complement(25942..26421)
                     /locus_tag="PKX09_06400"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOU17487.1"
                     /translation="MINEIIKMIAITFVPTLELRASIPYGIFAAKISWLIVFAVCVVA
                     NIIVGILIYLLMDLIIKIITKIPVITKVWDNYVAKTQQKIQKGVDKYGKWALAIFIGI
                     PLPGSGVYTGALAAYLIGLRFKKFMVANVIGVIIAGIAVTVICLTGASVSNIFIKQM"
     gene            complement(26499..27179)
                     /locus_tag="PKX09_06405"
     CDS             complement(26499..27179)
                     /locus_tag="PKX09_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU17488.1"
                     /translation="MNNRNYCIIIPAYQAVETLPPLLKYITMQFAENDIFIVDDGSTD
                     QTSDVINDYPQIHLIKQPVNRGKGAALHSGLREAYRLGYQFAACLDADLQHDPNYISR
                     FIAAQKQTGSDLVLGIRDFTFRTMPFHRILSNTITSFLISLRTGRRIHDSQCGYRLLN
                     LKIMQPFQFSENGFQFESEVLIKLLLAGFKYTEVKIPTIYRANHSSINNIKDTYKFIR
                     LFFKSYLW"
     gene            27273..27345
                     /locus_tag="PKX09_06410"
     tRNA            27273..27345
                     /locus_tag="PKX09_06410"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27306..27308,aa:Gly,seq:gcc)
BASE COUNT         8609 a         4870 c         5677 g         8004 t
ORIGIN      
        1 ttctattttc tcccgatgct caaagttttt aacgccgctt cttgatcata tttcccaact
       61 cacttcgcaa tttcaccgat attttcatat tccgatggat aaaataacaa aaccggatta
      121 tatcaacaac tttatatgga tttatgaatt aagcaggcta ataaggctaa cgatcaaact
      181 ttgggcaaaa tcgtaggaat aatatttctg ttgctcggag tagtaatgac tattataaat
      241 tttagataat gttgaacgat attagttcat ttcacgtccg acaatagaga atttctgcaa
      301 ggtgaaaaaa ttaaatattg tggaagttta tcagtatccg aaaggatcgg gaaatatatt
      361 tctatctgaa ctggaatagc aacgattgac cggaaataat tctaattgta atttggggat
      421 agcttttaaa ctctgcattc caatttcctg aaattctatg ttgttacaca attgatttgg
      481 aaatttaaat tattatacgc attatttata tgtgaaaaaa atgatagatt atagagcaca
      541 agggaagata tagtaaaaag aatatgagcg taaactataa aatattccaa aaagattgtt
      601 tagaatggct ccatgaaatt aataaaagtg ataagagcta tattcatctt acttttattg
      661 atccaccatt taatcaaggg aaggaatata gatattttaa tgatgctcaa cctgatgaac
      721 agtattggaa ctggattaga gatatattag cacaagttta tcaaataact gtagaaggtg
      781 gagcaatcta tttcatgcaa agggaaaaaa atacagaaca agttttaaga atacttcgag
      841 agacggggtg ggtttttcaa aacttaatta tatggaggaa aaagacctca gctgttccat
      901 ccgatattag attttctaag caatatcaaa ttattgcctt tgctactaag ggagaaaaag
      961 caagggtttt caataaacta cgaatagatt tacctattcc accggagtat aaatatgaga
     1021 gagctaatgg gatttatctc cctgatatat gggatgatat cagagaactc acctctggat
     1081 attttgctgg cgatgaagca ataagagatg tcaaaggaaa caggattcat acgcaacagt
     1141 ctccggtagc gctcctctta cggattattt tatcttccac gatgccaggc gatactgttt
     1201 tggacccttt tgccgggagt gggactactt tggtagttgc tcaccagata gaacgcaatt
     1261 ccataggaat agaaatagac ccggaatata tcaaaatcat tgaagaacga ttgaaacggc
     1321 tgagaagtgc agataatgtt ttagcttatt ataaatatta tagatatagc cctaatttgg
     1381 aaaaaatttg gtctagtgag aaacctgtta tcacggaaca aatgaggtta ttgtaaatga
     1441 atgaattcct cgatgcggta gagctattta tcggtgtcgt aaacggcttg aagaaaaagc
     1501 aaattaatct cagttcatcg ttagatgaga ttgtacttaa ggcgtgcggc actaaccttg
     1561 cttatgttga taatcggcgg agtgccaagg aagaattcaa ttttgatttc tgggatagta
     1621 taaaaaaaca atttaaggat ttgaaggatt tcatagattg gataaagaca tatggtattg
     1681 atgagcgatt tttatattct aaatctgaac aatttcctga ttttctatta aaggtaaaaa
     1741 agcatgataa taaattaatt tgtggtagtt tattagaact aaaagattcc aagggtggaa
     1801 gtatagcatc ttttaactca acacttccaa ctaaatacaa aagccttgat gaggttgata
     1861 taattatcgg acataagcgt atcatcaatc cctttggccc gatcaatggt gccaccctgc
     1921 tctatgccaa tcacgatggc caccctgaag cgatcgatcc gtattatctc gccgctcaaa
     1981 cctgtattcg tgaacgcctg aacgatcagc gcgattgaaa ggaataatta tggaattcaa
     2041 aattgacaaa accaaactca agatcgccat gctccagcat ggcattctga ctattgacga
     2101 actggccgcc aaactcgggt gcacccgcca gaacttctac cagacctata tcaaagatag
     2161 cgccctgcag aagaacatcc caaaactctg tgaagtcctc ggcgtcccgc ccgaagacat
     2221 tctcaaatga aaggatgatg tatgtcaaat tcaacattca aaattcaaca ttcaaaattc
     2281 tacttattaa ttttattttt tattgtatca ataactattg gattttctat tgggtattta
     2341 atttttaaaa atcccaaaca atcccaacca tctatttata atttttccgc tgaatcaaat
     2401 atttctaaat ttctattaaa acataaaaaa ttatctccgg ataaagtcac tttcatcaat
     2461 aaaaaaatat atgagatcga cgagtatttt attatttttg aaggtaaaat tagaaatatt
     2521 accaaatcag gtattcataa tgttatcatc ttttttgcta ttttaaatta tagcgattca
     2581 gatttctatt ctatgaatat gtggtgggaa gatataaaat atcatccatc attttactat
     2641 caatatttta attatttacc acccaatcaa gatatagatt ttaaaattcc aatttctcta
     2701 attggtcatt tcggtgatga tattgccgga ccttatacag caattgttca caataatatt
     2761 tatgtcgata tcattcaggc ttcagattta tctgaattcc ctatgattta ttaatctctt
     2821 aattcgctct ttggcatccg aattttccta tctctgcgac aaaaaagtcc acaaaacgta
     2881 aatccttgac tttctgatat tttcttgata aactggcgac gtgaagaatt ttacccgtta
     2941 ccagtgcgtc aatctggctt ttttgttagc cttagaaaac cataaccgcc tgccgggacc
     3001 cgcgagggat ggaaacaacc ttgtgtcaag gattacttcg attatggatg ggaagcttgc
     3061 ctccgatcaa aactatcata cttttcaaag aagaaatttt tattttataa gaacacataa
     3121 agatgatgac gataaagtta gaatttctat agtggacggg tcattttttg aaaccgtttc
     3181 taaagaacat cttattaatc aaatgcttct gaatattcta caaggtcatt tggagaaaaa
     3241 gggtataaat ataagccctg aaatccttaa tgaagttgaa aaatctattt cgtatgttac
     3301 ggatcagact ataattgccg cctcacagaa catagaaaaa gcatctattc gtcctcgtct
     3361 tagaattatg gcagaagttc atcctgaagg aaatcctcat agtagttttt acccagaaat
     3421 ttcagaacgg agcgtaaatc ttattatcca atcatctcca tataaagaaa aaattataaa
     3481 aacaatttct aaaaagattc ctgatgtgaa tgtgtttagt ataaaacata agaggaatgg
     3541 agaccacacg gttttccaga taaaattcta atctgtattt actcccctgt ctaaaaaatc
     3601 atttttatta taggttaaca ctcttgtctt ttatcatatc ccaaatcctg cgggactacg
     3661 tctatggtgg aaacgttaat aatattgaat aaactgaaat ttgaaatttc agtaataaca
     3721 aatatgctca actactataa gtataccatt aaaaagtgct tgtcattatt tcgtcagcca
     3781 cgcggcagct ctgcctttta ttccaaatta gactgccggt ttaatggcat tgtagaaatt
     3841 atcttgcccc aaaaatcttt accttgcaga gattgagcag cataatagct cttgtgtaac
     3901 atattgaagt gtatcatatt caagattatg attgatgcaa aaaaagttat taaagaaaaa
     3961 ataagcacta attttggctc agattcagag aaacttggcc tatacctgca ctttccattc
     4021 tgcttgcaaa aatgccgcta ttgtgatttt gcttccgaag atcaaagact cgagcgtatt
     4081 cctgcctata ctgaagcatt agtaaaggaa atagccttac gagccgatca acaggcggag
     4141 cgagctgaat tgcaaacaat ctacttcggt ggtgggactc ccaatctctt cggtataaat
     4201 aattttggta aagtgactca ggctattcga gaatattacc ttacgaaaaa tgttgcagaa
     4261 tttacggttg aaattaatcc gggtgtgtta acggctgaat ttatggagtc attaaaagca
     4321 gaaggtgtca accgccttag ccttggtgct cagtcatata atgacgatga attgctaact
     4381 ctgggacgta ttcataagag tcgagaaatt gatgtagcca ttgagcgatt gagacaaacc
     4441 ggattttcaa attttagcct ggattttatc tacggcattc ccggacaaac actaagtagt
     4501 tggcaaaaaa cagtcgaaga agccctgcga acaggcgctc agcatctttc gttgtattgt
     4561 ctaagttatg aaagaggtac accactatat caagcgcaaa tgaacggtga aatccaggtg
     4621 attgatgaag aactggaatg gcaaatgttc caaaccgccc acaacttgct ggtaaacgcc
     4681 ggatttcggc attatgaaat ttcaaatttt gccaaacctg gtttcgaggc gcaacataac
     4741 tcgatttact ggaagggagg aagatacttt ggattcggag ccgctgccca ttcatttgac
     4801 ggcgaaaaac gctggtggaa tgaacgtttg attgatgatt atattaaagc cctgcagaat
     4861 aactgtctgc cggttgccgg tcaggaaaaa ttaagtcttt ccgatcgcat aaccgaagcg
     4921 gtttttttag gattacgtac cgctgaaggt ttggatgttg cgttattgga aaaattgtcc
     4981 ggtatcgaat tcgcgtacat ttataacaaa ttaataaata agattggcaa taatcccgag
     5041 cagtttttta gtctgcagaa cggacattta attctgacga cgcgtgggtg gtttctctgc
     5101 gattcgatta ttgaaaaaat tttaacaatt atcgaggaga tgaaaaaatg ataatccgaa
     5161 aagtaaaaga agtaccagcg gaaccggttg ctgaaccggg cgctgagaat gttcgtcgcc
     5221 aggtactact ttccccgagt gaaggtgcgc cgcattttac cttgcgccga ttcgagattg
     5281 ctccgggtgg acaaactatg tatcatcacc acaattatga acacgaaatc tatgttctgg
     5341 cgggagttgg taaagcgcgt gggaggaatt ccgaagtccg aatcaatcaa gatgatgcga
     5401 ttctggtttt gcctaatgaa gagcaccaga ttgttaatac aggtattacc ccattgatat
     5461 ttttgtgcct gattccgaat cagtaacttt taaattaagc agataaaata aaaaggagct
     5521 gaaatttcag ctcctttgta aagtatttat tgatattctt atggtatcac gactttaaag
     5581 tcgttctctt ttttcaggtt atccagcaag tttatcagta attcattctg gcgcgaattg
     5641 attaaaatat tttcaatttc actccgaacg gtatccaacg gacggctctc ttttttacgg
     5701 tcgataactt taataatatg gtaaccataa ggactttcga caatatcgct gatactgccg
     5761 attggtaaat taaaagcggc gtcctcgaaa ggttttacca tttcgccgcg gccgaagtct
     5821 ttatataatc cgccgttatc ttttgagcct gggtcatccg agtatttttt agccagagat
     5881 tcgaaatttt cacctttttg ggctcgatca agaattcctt ccatttcgga atgaatctgg
     5941 gctttttctt caggcgattt tccctgcgtg atttttaaaa tatgtcgaac ggtagcggtt
     6001 tgatcggttt cgttataata cttttgaatg tcttcttctg taatattaac cgagtccatt
     6061 atcattttgt cgagatagct attgatagcc agattatcac ggatatcttc ccgtaaggct
     6121 ttaaaattaa tattggcatt gcttaagtaa ttaagaaatt tttccttacc gccgacctgc
     6181 atgtactgta attcaaggat gctgtcaacc tcggcgtctg atatttttat cccatttttc
     6241 tcagccgctt gtaagaggag ttttttctca gccaacgcaa tagcgtttcc cttgattatt
     6301 tcattcaatt gattggtatc aaggctcttt aattgctgaa taccatctcc gtacaactga
     6361 tgtaaagtgg tgaaaacatc tccgacggta atatcaaatt ttttagtagt aacaattacg
     6421 gtattttttt caggatcgag tatcggaatt tgctgggata gttcctgggc taattgataa
     6481 agctcggttc ctttttctaa tttggcgctt ttttctttct gggcacaaga aaagactaaa
     6541 aatcctacta atatgatcgg caatattctt ttcatcattt tctcctttac ttagaaatat
     6601 ttctaaaaac gcattaaaat tagataaaaa ttgaattaat tcataattat ttgtcaagga
     6661 attctttttg agaaattgga atgttagatg taacttaaaa ggcttattga agcccccata
     6721 gctcagatgg atagagcacc ggcctccgga gccggttgca caggttcgag tcctgttggg
     6781 ggtactaaat ctccgataaa taaaatcttg ccatattgta agttcattaa cctttttccg
     6841 aaagctaaac ttttatagta aaccggtttt aaatatatta ttggcgtgtt tggtctcggt
     6901 tgtttaaaga aaaattttaa gagaaagtcg ttcctactat ttacaatggt actgctctta
     6961 cgcttccagt tgttcagcca gcaatttgaa ttacgcgata tggctgagat gcaatggtac
     7021 aagggtagtc ttcatactca cgcccgagaa ggtgaaagcg attccccggt tgagtatgtt
     7081 gtgaaatggt atcgggaaca cgattataac tttttggtta ttaccgatca ctcgactgta
     7141 actattccac cggcctacgc cattgaaggt gatccctcct ttctatttat tccgggggaa
     7201 gaaataatgg gatacggaac tggccgtttg ttggagatta atgcctttaa tattaccaaa
     7261 gatatttcgc caattcatag cgaaaccgat ttagatgctc tccaaaaatg tattgacacg
     7321 gttcgaagaa tgggaggcat tccaatgata aatcatccca attatcaatg gcgactcgac
     7381 gcagacatta tattgaattc taaggactgc aatttatttg agttatataa cggttgtcct
     7441 ggtaccaaca attatggtga tgataatcat ccaagcacgg agcagatatg ggattttatg
     7501 ctatcggctg gaaagcgaat ttatgctact gctactgatg atgctcacgt atatcaaaaa
     7561 ttttctgccg aattatctaa cccgggtcgt ggatgggtgg tggtgaaagc taaaaaactt
     7621 gaggtaaacg aaattttgca gaatctcgaa gccggtttat tttattcaag cactggcgtc
     7681 gaaatttctg atttaatcgt caaacctgtc caaatcgaaa tctttatcca gatgagtggc
     7741 aaaaaccaat atacaactga ttttatcggt accgagggca aactcctgct acgcacgcaa
     7801 aataatccgg cagtctataa attgacttct aatgaaaaat atgtacgggc aaaaattgtg
     7861 gcttccgatg ggagttgtgc ctggattcag ccgatatttg ttgtgccata atttcggctg
     7921 aaaaattagg cagggcttat taataaaagc aaagatctaa agtttaattg atactgacgc
     7981 tcgcaattat tatccaaaat aatgggcaaa ttttggtaga ttgacagagg cttgatattt
     8041 taatggtcag tttttcggat tgttgtaaat tatcagttgc taaacttgat attgattgtt
     8101 ttttgatata caattgttac aaaaggaaaa gtacttatgc tgaaagaaga cttgatttac
     8161 cgtggaaaat ccaaagatgt cgtcgaaata aaaagtgggc catacactgg taaatatcgt
     8221 ttaattttta ccgatagagc tacgggttat ctggaaaatg gtaaacctgt ttttgatcct
     8281 ggatatgata tcgtcgtcgg cgatatcccc ggaaaaggtg cgattgcttg ccgatttgca
     8341 acgcattttt tcaaactgct gaaggataag ggtattcctt cccactatat tgatactgtc
     8401 agcgacaatg aaatgatagt tgaacccgct atcccgctta gtatgccggc ggaatcaccg
     8461 gagtttcccg gggcggctcc actcttgaat cttgaattta cctggcggaa taacgccact
     8521 ggtagtttct ggagaagata tccttttgtg cgaccttgcc gcaatattca tacgatcgtt
     8581 gaagcctgga ccaaaggaga tagtgatatt ctcattacgt ttgaagcgtt agcggaaacc
     8641 ggtgctatga ctaaagacga gattaagcag agtattaaac tggttaaaga gatcgccgaa
     8701 atcgttgcac aggaatttgc cagcaacaac ctccatgtca ttgatggaaa atttgagctg
     8761 gggcgactga agtatggcga tgggaaaatt gtaattattg acgagatctc tcccgatgtt
     8821 ctgcgggttt gcaagggcta ctctcccgat gctaatggta actgcacagt ttataaagat
     8881 tgcgccatga ctagttatgc cgatggaaag agaactatca agaataaaaa ccaggttcaa
     8941 gcggctgatt ttgtcaatat tttcctcagt cattgagtga ataagcgtat ttgagttctt
     9001 ttaagtgcta gtaagctctt ttaagaaata agagaatatg ggcgcagttc tataaaattc
     9061 tctgattctc gactattgaa ttaatcacct atttttaatt ttaattaaca gcaaatttgc
     9121 tcttgtctct aatagtgtat atcttacttt aggttatgga gaaatgtggg attaaaatta
     9181 taaatatgga agttgatttg agtaaatcgg aacgggcacg aaaaagcaaa ttttaccgga
     9241 tttttccgca cacggctgat ctgggagtgg aggtgtacgg cgccaatttg ctggaacttt
     9301 tcgaacattc cgctttggct ttgttcgatt tgatgactaa tgttgcgaat gtcatggagc
     9361 aggagacctg cgagttctcc gtagaagccg ctgacaggga attactcctg cgggagtggc
     9421 tcggcgaatt actttattat tccgtaagcc ggcaattttt attcaaatct tgccacatca
     9481 aacaatttgg cgagcaaaca ttgatagcta gtgcgagcgg agaaccgttc aacccgcaga
     9541 aacatatttt aaaacgtgag ataaagagcg ttacttatca tgaattacaa attaaaaatc
     9601 aaggtaatca ctggaaagcg cgctttgtcc tcgatattta aaggtcaata atggagcagt
     9661 taaaaataaa tcaaataagc gagcaagtat gggaaatacc gccaaccggc gcaatgcgcg
     9721 ttcctgggcg aatctatgcg aatgaaaacc tgatgcaggt tattcgagat gatcagagcc
     9781 ttaaacaggt cgcaaatgta gctcaattac ccggaatcgt aggctattct atggctatgc
     9841 ccgatattca ctggggttat ggattcccga ttggaggagt agcggctttt gatattaaag
     9901 aaggtgtgat ttctccaggc ggagtcggct atgatattaa ctgcggggtg cgtttgataa
     9961 ggacaaatct ggttgccgac cagctcaaaa ttgacattaa aaacttgatt gccgctattt
    10021 tcaatagtgt tcccagtggg attggctcgc atggagctat ccggaaactc agtcgccgtg
    10081 aactcgaacg ggtggcagtt caaggagcta aatgggcgat agaaatggga tacggaagtg
    10141 ctgatgattt acagtttatt gaagataggg gctgtcttgc ggatgccgaa ccgtccgtaa
    10201 tatcgcaacg cgcattcgag cggggtgccg atcaactggg gacgctcggc agtggtaatc
    10261 attttatcga gattggaaaa gtagtggata tatatgattc aaccgcggcg caggcgatgg
    10321 gtatatttca gggcaatctt gttttaattc tccataccgg ttcgcgaggg ttcggttacc
    10381 aaatttgcga tgattttatc aaatcaactc tacgtgccac ccaaaaatat gaaataaaaa
    10441 ttcctgacag gcagttggct tgcgccccat taaaatcaat agaaggacaa gattatctcg
    10501 gagctatgcg agccgcggcg aattttgcct tcgctaaccg tcaggtgatt agctcgttga
    10561 tttatgatag ccttttgaaa accttaaaaa ttagtccgaa cgagctcggt tttcgcccag
    10621 tctgggatat tgcgcataat atcgctaaga ttgaagaaca tcaatatggc gggaaaaaaa
    10681 tccatgtttg tgttcatcgc aagggtgcaa cccgcgcctt cggtcccggt aatcctgaat
    10741 taccagaaat ttatcaagca attggtcaac cggtactaat tcccggtgat atgggcacgg
    10801 cgagttattt atgcgttggg acagagattg ctatgcgaga aacttttgga agtgcctgcc
    10861 acggggctgg tagaatttta tcgcggcatc aagccaaagc caaagcccgc agtcttgatt
    10921 tatatcaggc attagcggaa agcggtgtct atgtaatggc ggaagaaaaa ggaacgatcg
    10981 ccgaagaaat gccatttgcc tataaggaag tcggcgaagt ggttgattcg gtagatgcgg
    11041 ctggcatcgt tcgtaaagtc gcccgcttca aaccactggg cgtaataaaa ggataaaagt
    11101 gataaacgca atgacaccaa gtcgttgatt tagcaagcga ttatcgcatt tgcattgaca
    11161 ttgcgatttt tcttgattaa tataggatta gtagctattt aaataaagaa aatgacttat
    11221 tctcaaaaat ttgtaacgat tgtcttaaaa gggcactgtt atgttaaagt tgagaattag
    11281 catctttacc gcttctttgt ttctcgttgg tgtcactctg gcgcaggatt ctgatttaga
    11341 taaatggctg gagttggatt tgagtgaatt gatgaatatc aaagtcacga ctgccaccaa
    11401 gaggagtcaa ctcattgata aagtgcccgc taccgtccgg attattactg ataaggaaat
    11461 tcaagagcga gcctaccaca atctggaaga tgttctaaaa gatctgcccg gatttcaatt
    11521 tcgcgatata ttaggtttta acagctattc ttttcaacgc ggagcaccca gtcagaataa
    11581 tctaatttta gtgatgattg atgggatcga gatcaatgaa ttgaattcgg gaggatttta
    11641 cggcggttat cattataatt tgcagaatat caaacaaata gaagtggttt atggaccttc
    11701 atcagcctta tacggaacga acgccatttc cggaataata aatttaatca ccaaagatgc
    11761 ggaagacgga ccgagagccg aagcgagtct aacagccggt tcatttggga cgcttttcgg
    11821 cgatatcagt ttgcgtctaa agccggaaaa tttgccgctc agcgtaactt tttccacttt
    11881 ttataaacaa actgaaaagg cggatttggg aggtactaaa ggcgacaaca attggtcaga
    11941 ggatatggaa aattttgaaa atgattatgg atttgattcc aaaatcaaat ataaaaacac
    12001 catacttggt ttgacacttc aggataaaca ggcttcccgc acgacaaatt ataaatcgct
    12061 cggcactaat taccttgact atggtacgaa ttttcacatt cgctttttga atgcctattt
    12121 gagcaacttg tacactaaaa aacctaactg gtcattgaaa tcgcaaatat attacagaaa
    12181 ctcgacggta atggataata caattgccta tattactact gatactggtc aggttggata
    12241 ttatcgtccg aattcactaa tcgggctgga agaaaaattc gattatgaat ggagcagtaa
    12301 agccaactta gttgccggag ttgtaatgga agccgaaaaa ctcgccagcg acttttctat
    12361 aacctatagt gatgacccag aaaaagctcc ggctaaacca gaggaaccgg attatgaaaa
    12421 taatactttg ataagtttct acgttcaaac ccaatacaaa ttttgggaat tagtcgaaat
    12481 gacggctgga gcacgccttg atcaaagttc ctattatggg acgattttga ctcctcgcct
    12541 aggttttgtt tatagccata agaattatac cggcaaattg ctttatactg aggcttttcg
    12601 agctccaaga ccctgggatt ataactgggg cattggtaat ccggatctga aaccggaacg
    12661 aatgaaatca attgaattag ccaataattt gaatttcaat caacgcttac gctgtgattt
    12721 ttcgctattc cataatcaaa tttatgacat gctaattgag aaagattcgt gctgggtaaa
    12781 tgccgaacgc ataaatacta atggtctgga attcaatctt gattaccgcc atgaaaaaat
    12841 acagctaaac ctgaattata ctttcacaga ttctaaattt tctgacggta aacaggtacc
    12901 tgaaattgcc cgccacggat tcaatgccgg agttacctat tcgttattgg cgaacctcaa
    12961 tgtcaacttg cgaggtaatt acctcagccg ccgtaaaaat cccgttttta tttctacaac
    13021 taaaagtcat tatgttgatc cgtactttgt gctaaatagt gttatcaact ataacccgat
    13081 cccaaatctg aaattgcaga ttgccgtcaa caatttatta aatgctcgct actaccatac
    13141 tagtaaccga ccgccagaac gctatcgtca accacaacga gcttttttgt tcaaattggg
    13201 gtatcaactt tgatatgatg aaatttgatg tattccaaag aaaattgatc tattgaatgc
    13261 cgtataaaaa aagaattatc gtctctattg tttgcttcct gatccttagt ttttccttta
    13321 tctacgccaa tttgccattc aatagtgaaa ctgagattaa tgacttaaaa gcggctttca
    13381 tttataattt tacaaaatat attacctggc cagatcttga acgtaataaa gtatttttta
    13441 tcggagtaat tggcaatagt gacattgtca attctttgcg acaagtagct gagaaaaaat
    13501 tcgttaaaaa tataccgatt aaagttgctc actaccaaag cgttgatgat ttgagatatt
    13561 gccatatttt attcatttct gcatctgaaa aaaatcaact cgatccaata ttagccaaac
    13621 tcaagaaaag gaagaccttg acgatcggtg acactcccgg attttgtgaa agtggtgtaa
    13681 tgattaattt ttttatgcaa gaagataggt tgaaatttga aatgaatctt gccaaattgg
    13741 aaagtgtcgg cttgaaagcc agttcccaaa ttaaaaaatt agcccggatt gttgagtgat
    13801 gccttcattt aatagccaac ttaaattatt tggtatgatg ctatggcgaa gatgaagtct
    13861 atcaatcggg gaatttttca gaagatgtct tttaggaaaa aactgattgt aattcagtta
    13921 attacagtaa gcacggttat cgtatttttc attatttttc aggtaataat aaatcagatt
    13981 gagtatcagc gagatgtcaa tgaaaagtta gaaacgacag ctcgcatcat tggctcgaat
    14041 tctattcctg ctattttatt ccttgataac gaaacggctg aaaatattct ggcttcctta
    14101 ggaagtgaaa gtgaagtcgt taatgcctgg ttatttgacc ataatagaaa tttgtttgcg
    14161 acatttgcgc gtgatgggtt tgagaattat gattatcctt tctttgattc cggtagatat
    14221 aagcttaaat ctcgctatct aatttattct tcagaactta ctcaagagga agttgcggtc
    14281 ggctacattc tgattcgcta ccagatgcat cctttatcct caataatatt agagagttta
    14341 gggctgggaa tagtcgtttt gctgattggt tttggactag ctttagttct ttcattttac
    14401 accggcaaga ccgtttccaa cccgatcatg gcattagttg atacgatcaa agaaatttcg
    14461 actcgacatg attattcaat ccgtttacca aaaacgactc aagatgaaat cggaatactc
    14521 tatgatggat ttaacgaaat gctggaacaa atccagaaaa aacaggcaga aagggataac
    14581 gccgaaaaaa aactcagaga agcaaacaca attatcaatc gcagtccggt cgtagctttt
    14641 acttggtata acaaacccac ttggccagtc gagtttgttt ctgaaagtgt catcaatctt
    14701 tttggctata ccgctgaaga gtttatacaa ggcaaaatat cgtatgaatc atgtatttat
    14761 cctgaggatt taccgagagt taatagtgaa gtaattaagt atagtcaaac acccgacgtg
    14821 caatctttta aacatgaacc ttatcggatt gttactaaat caggcgcaat aaagtggatt
    14881 gatgatctat cttttatcat tagaagtgaa tccggagaaa ttacccatta tcagggcatc
    14941 gtttctgata ttactgaacg tatggaattc gaagaattgc tgaggcagag cgaaatccgt
    15001 tatcgtcgaa tatcttccgt agtatcggat tatgtttttt cggccaaaat caatcaagat
    15061 ggtgttctgg taaccgactg ggttggcggt gcctttgaga ccataactgg atttaccttc
    15121 aaggaattta gtgaagccgg tggttggaga gccaggctcc atccggacga tcttgaaaaa
    15181 gatgatcatg attttgaaaa attgaaaaag aatgaaccga tccaatccga attacgcttt
    15241 tatactaaat ccggtgaact attatggtta agagtttatg cttatccggt ttgggattat
    15301 aaaaacaata gattaattgg tgttcatggc gcggttcaaa atatcaatga gcggaagaaa
    15361 gctgaacaag ctcttcagga aagcgaagtc cgttaccgat tgctctttga atctaatccg
    15421 gcgccgatca taatctatga acataatacc tataagattt tagcggttaa tgaggctttc
    15481 ctccagtttt atggttattc caaagatgaa attctggcaa tgcgcttgac ggatctttat
    15541 ccggcagatg aaaaaaagaa aatagttaaa atgctaaatg atttatatga ttatcaagac
    15601 gctgtcgaat ggcatcactt taaaaaagat ggaacaatgg taaccgtcat tttccgttcg
    15661 aatgatttga aatatcaggg gcatgatgcc cgtgtcgcag ttatcactga cgttactgaa
    15721 cgaaaattag ccgaagaaaa aatcaaaaat ttgaacatag agctggaaaa aagagttact
    15781 gaacgaactg ccgatttagt aaaagaaatt gaagaacggg aaaaaattgc actgactctg
    15841 gaacaatccc gagaatctct gagaactatc attgaatcca taccatttcc tgtagtgctg
    15901 gttaatcaag atcgaacaat cagggatgct aatcaggcta ctcttgactt actcgggttt
    15961 aattcgttcg atgaaattgt tgggaaaaat tgttatgaaa ttttatgtat ttataatagc
    16021 gatacttgcc caatcttaag cccagatcag atagtagata aaaaagaaac agtgatcata
    16081 aataaagata aggtaaacgt tccagtacta aaaagtgcag ttccaattaa aatcgaaggg
    16141 gaagaggtac tgctggaaac ctatgttgat atactcaaaa tcaaagttat ggaaatggaa
    16201 ctgattcagg ctaaggaaca agcccaggcc gctacccaag ccaaaagcga ctttctggct
    16261 aatatgagcc acgaaataag aactccgatg aatgcgatta tcggtttaac gtatttagcc
    16321 ttacaaaccg agttagacgc tcgacagcat gattacataa ctaaaataaa atcatccgct
    16381 cagaatttgc tggaaatcat taacgatatt ctggactttt ccaagatcga agctcgtaga
    16441 ctaaaactgg agaatattga ttttaatctg gaaaaagttt ttcaggatac cgccaatgtt
    16501 gtaaccttta aagcccatca aaaaaatctg gaaatcatct tcggcataga taaaaatatt
    16561 ccgcgctact tagttggcga tcccttgcgt ctgcatcaaa tactggcgaa tctaactaat
    16621 aatgccgtca agttcactga taccggagag attattgtcc gggcagaaat ggatgaggaa
    16681 tatgacgaca cagtaaaaat aaagttctca gttaaagaca ccggcattgg tcttactaag
    16741 gaacagcagg ataagctctt tcaatctttt actcaggcag attcctccac taccagacgt
    16801 tatggcggta ccggtctggg attggctatt tctaagcaat tgaccgaact gatgcaaggc
    16861 gatatttggg tcgaaagtgt ttatggaaaa ggcagtacct tctactttac cgccgtcttt
    16921 aaaaagcagg aaaatcaaaa aatcgaagaa tttgttctta ctcccgattt acgagggtta
    16981 aaagttctgg tttgcgacga taataaaagt tctcgtttaa ttatcaaagg cgcacttgag
    17041 actttcggtt ttaaagtcgt gctggcttct tctggcaaag cggctataga aattttaaag
    17101 aaacacaaaa gcaaaccatt tcagcttatt ataatggact ggaaaatgcc tgaaatggat
    17161 ggtatcgaga ctattcaaac aatccgaaag gatcctgaaa ttccctctgt tccagccatc
    17221 atcatggtca gcgcttatag tgaagaaaaa atcattgaca gaattgaaaa aatcggaata
    17281 gatgcatttt tgttaaaacc ggtcactaat tcgacattat ttgatacaat tatgcaggtc
    17341 tttggcaaaa ccactcctag gcgcaaacgc caagccgaca gagggcaaag acttaaaagt
    17401 gagttggcgc ttatcaaagg cgcccggatc ttagttgccg aagataatga aatcaatcag
    17461 caggttatag atggattgct tactgcttat ggaatggacg ttatcgtagc ggaaaatggc
    17521 cgcgtcgccg tccagaaatg tcaggaaatg catccttctg aactcgattt agtttttatg
    17581 gatcttgaga tgccggtatt ggatggttat gaagcgacca gggcgatccg tcaaattgcg
    17641 ggatatgaaa agctaccgat tgtcgcgctg acagccgatg ctatgtccgg cacaaaaggg
    17701 aaagtattat cggcgatgat gaatgattac attaccaaac cggttgatcc gaacgaggtc
    17761 tataacattc tggttaagtg gattcccgct aaaagaaaaa aaataaataa aggtgaatct
    17821 gaacagcagt tggctaaaaa tctaaatttt ccagaagtat ctggtattga tgtccagtct
    17881 ggtttgagac gggtcgccgg taaccagcaa ttatacggaa agattctgca aaattttgtg
    17941 cgtgaaaata taaatctgat cacaagcctt caaaattact tggtagataa tgacttagaa
    18001 gcagttgaac atctggtaca ttctctcaaa ggaagttccg gtaatatcgg agcccagaaa
    18061 ttatatgaag tcgcttctgc gtttgaaatg gaattaaaat caaaatctcc tgattctaaa
    18121 aatattatgg aattaattca gcaattgagt atggaacttg agttggtttt taatacaatt
    18181 caaaaaattg aattgccagc cgaaattatt agttctaata aaaccaataa acccgggaaa
    18241 tggggaattg agcaggaaaa gaaatgccag gaattaaaaa aatacttggc tgagtatgac
    18301 gcaagagccg gtgacatttt ctatttgatc aagagtgaat taagccaatc aattcctgaa
    18361 gtagatttga caaaaatcgc cgaatctatt gagcaatacg attatgatca agccctaagt
    18421 attttagaga aattaattga actgggcgac cagatcaata aggattaata tggagaataa
    18481 aaaacctaca atcttaattg ttgatgacgt agcaacgaat ctgaattttc tgaaagggct
    18541 actaataaat gattacgaca taaaagcggc caataacggt caattagcac tgaaaatcgc
    18601 tcgaatggga gcagggcccg atttgattct gctcgatatt atgatgcctg gtatggatgg
    18661 attccaagtt tgtgaagagc taaagcgtga tccgcgaact caggatattc caattatttt
    18721 tctgactgcc agaacggcaa ccgatgatat tgtaagaggc tttaaatccg gtggagttga
    18781 ttatctgact aagccgttta atccgccgga actactggcg cgcgtccgta cccatctttt
    18841 aattaagcaa caaaaagata ttattcttga acagaaccgt gagcagaaag aattattaca
    18901 tattttatgt cacgaccttg ccaatcactt tactgttata agtatgtccc tcgatttact
    18961 gaaaaatgat tcagctataa ccgattacct cagtaaaata aaaactgcga ctacccatgg
    19021 tattgacatc attaatctgg tcagggaaat gcgtgctctt caggagaaaa aaatagaaat
    19081 aagtgcggtg aacctgagcg aatgtttggc agaatcggtt ttgctacttg gtgatcgctt
    19141 tgagcagaaa aatgttaaac tgttgataga tattgaccat agcctatggg taatggctga
    19201 gaggtgttct ttaatcaatt cagttattaa taatttgatg accaatgccc tgaaattttc
    19261 ctttgaagga gggcaggtcg aaatttcggc ggaagaagaa gatgacgccg tgattttcaa
    19321 aatccaagat catggaattg gtatgccagc tgagttgatg agccagattt ttgatataac
    19381 taaaattaca agtcgccccg ggactaaagg cgaaattgga accggttttg ggatgccatt
    19441 agttaaatct ttcgtcgaat tttatggtgg cgaaatcgag gtggaatcgc gggatattaa
    19501 ggaatatgcc gacgatcatg gtactttaat cacaattaaa ttcattaatg ctaaaaataa
    19561 ttaggaggag caatttatgc tgaaagtgtt gattgttgat gacaataatg agagtcgttc
    19621 cctattagag tcgtttctta accgtcatga ctaccaggta ttttctgcgg ttaacggcaa
    19681 attggcgctg gatattttgg aggaaaatga tgttgattta atcatttcgg atattttgat
    19741 gccggaaatg gatggctata tgttatgcaa tattgttaaa tctgatccgc gctggaagga
    19801 cattccattt attatctact cggctacttt tacttcacga caagatgaga ttttcgcctt
    19861 gaaattaggc gcttcgcgat ttatacgtaa accggttgaa tcggaccttt ttctgaatat
    19921 tattcaagaa gttctgcaaa accgaattga agggagtctg gaagtcgcct atccaaggtt
    19981 tcaagatgaa aaagatgctt tcaagttata tagtgaacgc ataatcaaga aattggaaaa
    20041 aaatgtaagt gaacttgaag aaagtgagga gcattataga gccatttttg aaaatgcggc
    20101 gattgggctc tatcgttcta ctccggatgg cagaattttg atggcaaacc cgactctggt
    20161 aaaaatgctc ggcttcgatt cttttgagga actatcgcaa gtgaatctcg aagagggagg
    20221 atttgatctt aagtacccac ggtcggaatt taaaaaacga atcgaagctg aaggaaaagt
    20281 ccagaattta gaatcagtat ggaagcgtaa agataattcg attattcacg ttcgcgaaag
    20341 cgctattgcg gttcgtgatg cgagcggtgt aactaaatat tacgaaggca ccgttgagga
    20401 tattacggca cgcaaaaagg cggaagaaga attaagggaa agtgaaaaac actatcgcga
    20461 gttaattgat ggaatgatcg agacagtctg gattatagat tttaacggcg atcttatgga
    20521 cgttaaccga actgcaacgg aagtcttagg atatactaag gaagaattac tcaagattgg
    20581 gcttttcggt attgattcct ctttaaaaag ggaagatatc aagactcttg cccggtcaat
    20641 gaccaaagat aaaatccaga tatttgaaac tacccacaaa accaaagaag ggaaatctat
    20701 cccggtggaa atttattcca gtttgattga ttatcacgga caacctgcca ttttgagtat
    20761 tgccagagac ataaccgaac gtaaacgggc tgaagcggcg ctaagagaaa gtgaggagcg
    20821 ctaccgggca gtggtggaaa attcacaaaa tgcgattcta atagtcggtg aggattttaa
    20881 gtttatttat gttaatgatg ctctttgtac acttgttggt agatctcgtg atgaaatcat
    20941 tggacatgat ttcagagaat tcctggatga tgagagcaaa gcactagtag gagagagata
    21001 tctcaaacgc cagcgcggtg aggatgtacc caatagatat gaattcaatg tcatcagatc
    21061 aaacggagag atacggagag ttgaaattag ctctacagtt gttaaagata cgcaaggaaa
    21121 tcttttgaca atcgctcagt taatggatat gaccgaacgt aaacgggctg aagaagattt
    21181 agtccggata atggaaagcg ctcaggctat tctctggcat tcaccggtag tcagacagga
    21241 gggaactgaa ccggaatttc tatggaaaac tcgctatttc aatctggacg ccattaaaaa
    21301 tattatcccg ctccctgatt atccaactca tgatattaat gatcgttatt attacagtat
    21361 tttagaagaa gaccgccggg caatggatcg agtcagttct gctgcactta agagcggtgc
    21421 cgatcgctat agccaggaat ttcgcttgcg agatgcaaat ggtgtaattc actggatgta
    21481 tgaagacgcg cgaatcaaga aaattgatga atcgcatttc gaggtagttg gtgtcattac
    21541 tgaggttacg gaacgcaaga aagccgagat agccttaaaa ttaagtgaag aaagattcaa
    21601 gtcattattt gaatataata gtagcgccgt tgttatatat gaagtggttg ataatggtaa
    21661 tgatttcatt atcagagatt taaataaagc cggtgaaaaa attgataaga taaaaagaag
    21721 tgaggtagtc ggtaagagct tattggaagt attttcggga gtgaaagatt ttggtttatt
    21781 tgaagtttta caacgtgtct ggcggacggg ggaaccggag aattttccag tgacatttta
    21841 taaggataat cgcatctctg gctggagaga taactatgtt tttaaactgc cgagcggcga
    21901 agttgtggcg gtttatgatg accttaccaa acaaaagcag gctgaagaag aggttcgtaa
    21961 atctgaggaa aatttccggt tggtgtggga aaattccgtg gatggcatgc ggctgaccga
    22021 tgaaaatggt atagtcataa tggtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22141 nnnngaacaa aaaatgcggc agatcgaaaa ggaagaattg caaagtcaga ttgttcagct
    22201 ccagaaaatg gaagcggtcg gcaatctggc aggtggagtt gcccatgatt tcaataattt
    22261 gttgacaatt attcagggat atactcaaat gctaatggcg caaaaggaca agactgaacc
    22321 agattatcag ggattaaaac atgtattgga tgcctgtgat aaagcggcta atttgaccag
    22381 gcaattattg cttttcagcc ggaaacaacc tatggaattc aaaccggtca atatcaacaa
    22441 cactataact aatctactaa aaatgatcaa acgccttatt ggtgaaaata ttaaaattat
    22501 cactaatcta aaagagccta tccaaaatat cgaagccgat gaaggtaata ttgagcaggt
    22561 tatcattaat ttagtagtga atgcgcgtga cgctatgccg aacggcggta ccttaaccat
    22621 ttcaactgac aatgttgctc tttcagccaa agagtgccag acaatcaagg attcccaccc
    22681 cggccggttt gtgcgggtgt cagttgccga taccggtcag gggattccgt ctgatttaat
    22741 cgaaaaaata ttcgagccgt ttttcactac gaaagaagcc ggtaaaggaa ctggtctcgg
    22801 attatcggtg gtatatggca tcattaagaa acataacggt tggatcaatg ttcagagcga
    22861 aattggtgcc ggaacggttt ttgacattta tctgccagca actgagataa cggtggatga
    22921 taaagctcat ctatcggctg aaactcagat gaaacaaggg aatggagaaa agattttgac
    22981 tatcgaggat agtgccgacg ttttgaaatt tctagcggaa actcttcacc aaaatggata
    23041 tactccgatt acggcggcta atgccgctga ggctattgag caattccaga acgaaaatgg
    23101 acaaattgat ttgatattgg cagatgttgt attgccagac aaaaatggag taactgtatc
    23161 tgaagaaata ttagcccaaa aagaagatat tccggttata ctctatagtg gctatgctga
    23221 agaatctatc atacaattga tcaagaaaaa tcccaaattt gtttttatcc agaagccttt
    23281 taaaatgaag gaattattgg atttgatata tcaaaaattg cattgattat tgagtgtagg
    23341 acaggcattc ttgcctgtca attttaatgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23461 nnnnnnnnnt gacagcacca agcttatgct cgcggtggtt ggcgaatacg gtgcctgtca
    23521 attttaatgg tgggtaatat ctaagtgaca gcctggaaag gctgtcctac ggggttaagc
    23581 ccacggcttt tcataaaggc tatctatttt acctgattta aaatttcctt caaaacatct
    23641 ttatggacag tataggtaag cattttcaat ttgatgtaat cttcgcgata aaaataatag
    23701 ccttcatatt ttccccaacg tgaactgcga gccgagtcct ttactaaaaa ccagtaacga
    23761 tcgtcaatgc gggtatatcc gacaagatgc aaaccgtgat cgtcagtcgt agttttatta
    23821 taaattcgga attcgcggga gtcctgatta atatatttaa acggaatatc gaaatccgga
    23881 atgaaagcta catcctcaaa gcccagataa cccggttcac tgacatcgcc gccaatcgcc
    23941 agactatatc ccccggtaat cgcttttttc aaaaatttat accattctgc cagtggaata
    24001 ttgatataag acgaatcatg ccaccaattg tcctcgactt taaattccgc tcggatataa
    24061 aatggaacag cggtcgtgga cattacgtcg caataatcgt cgagttgcaa ttttagaaca
    24121 ttattcaaaa aagttaccgg agtatattcc tgaccttcgt agataaaact ctggggtggt
    24181 tcgccgagat atttatttag aatcagttta atactattaa gagcgatttc ttcgtcccag
    24241 taattgttgg ttttacaata ttcaagataa ttgtgaattt ctgcgaaaag gcgagtgtga
    24301 tcatgtttat cgccatttgg aagtccggta tagatctccg ccggaaccgc tccgtatttt
    24361 ttcataatgc ggaaaacgcc gttgacttcg ccaccttctc caatgaatga atcgcctctt
    24421 tcgcgaatga accgcgccgc tttctccaga tattcatagt aaatggtatg taattccgat
    24481 attttgattt tttgtccggt cagacgcata acctccgatt ccaaaaaaga tgttccgcaa
    24541 aagcaccaac acataccgga ttgatattgg gcgataggag ggaagtggtc aattgtttta
    24601 aagacattga gcgaagctgg tttttttact ccggagaggt cggattggag agaaagtaca
    24661 tcctttttgt taatttttcg gcgcgcttcc tgttgagtgt gaattgccgc agtcagcgaa
    24721 tcctcacgag caatatcttt ctcacgttgc tctttcatct gttccagtac cgggtcgtgg
    24781 tatctgggtt tatataccgc tttattaagg ctttgcgcgt ttaatgtaat gaaaacaaaa
    24841 aggttagtaa taaaaatcag aaaactttta cgtaatagtt tcatttttaa actccgtttt
    24901 agtggttatt aatttcaaaa ttgttttaat catttcaata aatgttaaga aattcataag
    24961 acgttttcaa ctgaaaattg aatcgaactg taaccactga agaggaaaaa ttatctaaaa
    25021 acaataaatt aattaacaaa aaggagtcag taatggaaat gataatagat ttcccgaaag
    25081 ggttacgagt agatgctcat ttaggtaagt ttaccattcg gactgatcag ccgctcagct
    25141 cgggtggcga agaaacggct ccgacgccgt ttgcattatt tttggcttcg ctgggaacgt
    25201 gtgccggggc gtatgtcctt agtttttgca gacaacgcaa tttgccgacg gaaaagattc
    25261 ggctggtgca acgtacggag cgtgatacgg ctacaggtat gattagccga atccaattag
    25321 agattcaggt tcccgaggat tttcccgaaa aatattatga tgcgctggtg cgttcagcta
    25381 accaatgtat cgttaaaaaa catctggaaa atccacctca ttttgaagtt tttaccaagg
    25441 ttgtataaat gaataaaatg gaaaaaaatg gttggcaaaa aattatatct gttctaaatt
    25501 aaatcgcttt ttgagtaatg cgcccgtagg ccaactggat agaccgtcag gctacggacc
    25561 tgaatgttga gggttcgaat ccttccgggc gtacattaaa ataaaaacag ccggtgacaa
    25621 ccggctgttt tgtttagatt aattagagta aaaaaaactg cagaaggtgc gttcctacct
    25681 gtcagttgat attaattaat aattggtcgg ggcgcccgga tttgaaccgg ggacctcttg
    25741 gtcccgaacc aagcgcgcta accggactgc gctacgcccc gcaannnnnn nnnnnnnnnn
    25801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25861 nnnnnnnnnn nnnnnnnnnn nnnnattgtc aaaaaagcgg ttaaatttat tattatatat
    25921 tagaatattc aaacagaaag gctacatctg cttgataaaa atattactaa cactggcacc
    25981 cgttagacaa ataaccgtta ctgctattcc ggcaatgata acgccgataa cattagctac
    26041 cataaatttc ttgaacctta agccaatcaa ataagccgcc agcgcaccgg tataaacacc
    26101 tgaacccggt aaaggaattc cgataaatat tgccagagcc catttaccat atttatcgac
    26161 ccctttttga attttttgct gggtttttgc cacataatta tcccagactt tggtaataac
    26221 gggaattttc gtaataattt taattatcaa gtccattaat aagtagatta gaataccaac
    26281 aattatatta gcaactacac aaactgcgaa tacaattaac cacgaaattt tagccgcaaa
    26341 aatgccgtac ggaatcgaag cccgtaattc gagtgtgggt acgaaagtta tcgcgatcat
    26401 ttttatgatt tcatttatca ttcattttcc aaatagtaat cggcggctaa attacaataa
    26461 tttgactgtt ccggaaatag ccgaattata aaaattttct accataaata actcttaaaa
    26521 aataggcgta taaatttata ggtatctttt atattgttaa ttgaactatg attggcacgg
    26581 tatatcgtcg ggattttaac ctcggtatat ttgaaaccgg ctaagagtaa cttgatcaga
    26641 acttcgcttt cgaattgaaa accgttttcc gaaaattgaa atggttgcat aattttaaga
    26701 ttaagcaaac gatatccaca ttggctatca tgaatgcgtc gtccggttcg gagggaaatt
    26761 aaaaaggaag tgatggtatt ggatagaatt cgatgaaacg gcatcgttcg gaaagtgaaa
    26821 tctcggatgc ccagaactaa atcggagcct gtttgctttt gagctgcaat gaaacgacta
    26881 atataattag gatcgtgttg taaatcagcg tccagacacg ctgcaaattg gtaaccaagt
    26941 cggtaagctt cgcgcaaacc tgaatggagt gcggctcctt tacctcgatt aacaggctgt
    27001 ttaattaaat gaatttgcgg ataatcgtta atgacgtcac tcgtttgatc tgtcgaaccg
    27061 tcatctacaa taaatatatc attttcagcg aattgcatag tgatatattt cagcagaggc
    27121 gggagagttt ctaccgcttg gtaagccgga ataataatgc aatagttacg attattcata
    27181 ctgaaaatta gattatttag cagataccgt caaatgtaaa aattattgca taaagtaggc
    27241 gaaaagttta aatttacgcg ctttttattt atgcgagagt agctcagctg gtagagctcc
    27301 acgttgccaa cgtggctgtc gcgggttcaa gtcccgtctc tcgctcaaca aatatctcaa
    27361 aacaaaagca atgagcttag cgttgctccc gaccgttcgg gatcacgggt tcaagtcccg
    27421 tctctcgctc aacaaatatc tcaaaacaaa agcaatgagc
//
LOCUS       DAOSMP010000030        26410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_1842_length_26410_cov_20.957314, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000030 DAOSMP010000000
VERSION     DAOSMP010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 26410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26410
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1842_length_26410_cov_20.957314"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PKX09_06415"
     CDS             <1..786
                     /locus_tag="PKX09_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOU17489.1"
                     /translation="AIATYTAPKDSRIYGTYEVDVTSVLQYIKEKREAGIHLTITYFV
                     AAAIARALYEDMPEVNCFIRRGQVVARENADVFLSVNVGGEAMTGLVLRKCQELSATE
                     IGQIIQKVVEKKRDGKEEEGAFAAKDKLAKIPWPFRRPVFLFIKWWIFDMGWTFPFLK
                     IKPDPFGSIMLTNIGTFGLHTGMPALFPIGKLPAVVAMGKIAKKPVVIDDQIVIRDIL
                     PLTGTFDHRIVDGYQAGVLTKGVERRLQDPEALNRPNNSNNSN"
     gene            812..997
                     /locus_tag="PKX09_06420"
     CDS             812..997
                     /locus_tag="PKX09_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013466645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOU17490.1"
                     /translation="MSEIESKILSAIEKAGKPLKAGEVAEMTGLTKDEVAKVIAGLKK
                     QGKVESPKACYYQVKKS"
     gene            1159..2562
                     /locus_tag="PKX09_06425"
     CDS             1159..2562
                     /locus_tag="PKX09_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HOU17491.1"
                     /translation="MDLWNFTLNFKDIVLDFSFMSVFLVAGTVFRRYGLFFQKYLVPN
                     NLIAGFIGLILGSQVLNIVPISEERMGLYVYHLLALTFVAIGLRGEKTSWGKGPLSFS
                     LSMLSSYIIQGLIGMLVCFGFVYTIRPDLFIGLGLLLPMGFGMGPGQAFTIGKGWEKY
                     GFTGGGMAGLTIAAIGYLVAYFAGMVIVNKGIKRGETALIQGMDSITSDMRTGVIKKG
                     EKPIAGFLTLSMEAIEPLAFQVAVIGVVYLISFWVAKGVEIALLKIGAGGFVATVWAF
                     HFVIALLVALAMRKFLDKIKKSYLIDPGLMTRTAGLFVDYLIVGSICGISLKVIWMYW
                     APILVMSVIGGFATYYLLKYVCYRAFDDYHFERFAAIFAEMTGTLNSGLVMIRVTDPE
                     FKTPVAEDLVYSSGIALFLGLPLLILLNVPMNFFNNSLKGYWITFGLLIVYGVILWIF
                     WRAIGFIHFKKNPKSKV"
     gene            2651..3841
                     /locus_tag="PKX09_06430"
     CDS             2651..3841
                     /locus_tag="PKX09_06430"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOU17492.1"
                     /translation="MKKRIGILTGGGDVPGLNPAIRGITLRALKEGFEVIGIRRGWAG
                     MINIIRDKEADNSQNFIMLNQEIVNKAGRTGGTFLHTSRTRPSSVKLDAVPEHLRDKY
                     TAEVNDLTPEVLKNLDFLGIDYLIPIGGDDTLSYGVTLHKAGVKVIGVPKTMDNDVPG
                     TDYCIGFSTCVTRTLELTHSLRTSAGSHERFLVIEVFGRYAGFTALLPTLAGAANRCV
                     IPEYKFNMERLTELLVQDRFTNPSRYSVVLVSEGAMMQNQDQMMFQSEETDAFGHKKL
                     GGIGDVVARQLKELSGKYNNGHKINVISQKLGYLVRCGDPDALDSIVPMAYGNIAFEL
                     IMNGRSGLLVNLRNGVYGHTDLEVVTSTKKVVDVEKFYETDRLRPKYKSFENKSFFIM
                     TSDK"
     gene            complement(4035..4250)
                     /locus_tag="PKX09_06435"
     CDS             complement(4035..4250)
                     /locus_tag="PKX09_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17493.1"
                     /translation="MFIKLFELLPGFSELVNSSLSKLKKVTTEMPRKATAISIREVIA
                     EPHVFFRKKMKGIFSISLKKGGKIDPS"
     gene            4275..7781
                     /gene="nifJ"
                     /locus_tag="PKX09_06440"
     CDS             4275..7781
                     /gene="nifJ"
                     /locus_tag="PKX09_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOU17494.1"
                     /translation="MSKTMKTMDGNTAATHIAYALSEVAAIYPITPSSVMGELADEWS
                     AQGRKNIFGQPLRVVEMQSEAGAAGAVHGSLSAGALTTTFTASQGLLLMIPNMFKIAG
                     ELMPAVFHVSARAVAGQALSIYGDHSDVMATRTTGFGLMASGSVQEVMDLSLVTHLAS
                     LKASLPFVSFFDGFRTSHEIQKIEVIDYEMIKSLVDFTDIDRFRARCLNPEHPQLRGT
                     AQNPDIFFQVAELANKYYDAVPEIVAEMMRKVGEVTGRHYNLFDYAGDPNAERVVVIM
                     GSGSETVQGVAEYLNARGEKVGVLKVHLYRPFSAKHFMAALPKSAKRIAVLDRTKESG
                     AFGEPLYTDVCAVLQREKDNRLVVGGRYGVGQKEFSPAMAKAVFDNLKLPEPKNGFTV
                     GIEDDVTFRSLPVTEDIDTVPEGTVQSIFWGFGSDGTVGANKDAIKIIGDNTDLYAQG
                     YFSYDSKKSGGLTVSYLRFGPKELHTPYRINLADYVACHKQSYINNYDMLINLKPGGI
                     FVLNTLWDDAELDANLPAAMKRAIATKKVRFYAVDANRIAGEVGLGGRINMVMQTAFF
                     ALSKVLPFEEAIKHLKNAIQKTYGAKGEKIVKMNWQAVDSAINAIREVKYPASWAEAK
                     ETAESPKPVPDFVKKVLQPINAFNGDNLPSSVFTPGGIFPTATTKYEKRGIAIEVPEW
                     IKENCTQCNQCSFVCPHAVIRPVLVTPEEKAKAPTTFETIKAIGKGLENYEYRMQVSV
                     LDCTGCGNCADVCPGRKNEKALVMKSFESQLEQKENWEYFETIPVKANVMRKDTIKGS
                     QFQQPLFEFSGACGGCGETPYLKVITQLFGDRMIIANATGCSSIYSGSVVCPYCVNET
                     GYGPAWANSLFEDNAEYGFGMALAIIQRREYLAEIINEALKSADGELAEAFKAWLDGK
                     DDAELSRAAGDRIKAALADKWEGNKLLTEIWKNRDILTKKSIWIIGGDGWAYDIGYGG
                     LDHVIAMGYDVNILVLDTEVYSNTGGQSSKATPLGSVAKFAYSGKKTSKKDLGMLAIN
                     YKNVYVASVAMGASANQFLKSALEAEKYPGPSLIIAYCPCINHGIYKGMLSQEESRQA
                     VECGYWPLYRYNPLLKAEGKNPFQLESKEPNGKLMEFLDGEVRYATLKKSFPEESEKL
                     RKALSEEVIERYQKYKRMAEES"
     gene            8023..9465
                     /locus_tag="PKX09_06445"
     CDS             8023..9465
                     /locus_tag="PKX09_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU17495.1"
                     /translation="MTFLNFLFPPDDPLSDILKKISTVAECNHKRVFLVGGVVRDRLL
                     GIDTKDIDISVIGNGIEFAHKLAKSLKVDNVIEYPRFGTAMIPYHDKLIEVATARMEE
                     YETSSRKPQIIESSLRDDLARRDFTINAMALSLNRENYLELTDYFEGLKDLNAGIIRT
                     PLDPITTFSEDPLRMLRAVRFATQLNFRIEPNTFKAIEQVRERIKIISQERITDEVTK
                     ILMVPGNPSRGFELLQESTLLDLILPEIAALDGVDQRNGYHHKDVFKHTLQVLDNVAR
                     VSTKFELRLTALLHDVAKPLTKQFFPDKGWTFYGHEELGAQMVGEIGKRLRLPNKTIE
                     YAAKLTRLHLRPIAIASEGITDSAVRRLIVEAGDELDDLILLCRADITSKNIQKVEVY
                     RANFDRVVNLIESVREKDRLRAFQSPVRGEEIMRFCNLPPGPAVGFIKSAIEEAILEG
                     EIDNTYDAAHTFLEKNWEKLLKKFNLKPKA"
     gene            9530..10225
                     /locus_tag="PKX09_06450"
     CDS             9530..10225
                     /locus_tag="PKX09_06450"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOU17496.1"
                     /translation="MSDDENKTIFGKIISIFWEPSATFRALVIKVSWLDIVVPLLLVL
                     LVALAVMPYITPLAVEEQKDYIAQSDRLTEEQKAQAYDRIDQQAGSARAYITSAVFLV
                     IRTAVVAGVLILVSNFLFGGEVKYKSMLAVSAYANLVDIIANAVKVPLMLSQQTVRIY
                     TSPALFLQDNSTFWFRFFANLDLFVLWKVILLGIGVGIITKVKTSKSIWTILICWIIY
                     CLAVAGLGGLAKV"
     gene            10248..11552
                     /locus_tag="PKX09_06455"
     CDS             10248..11552
                     /locus_tag="PKX09_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU17497.1"
                     /translation="MKRVIFILLIGQILAYGADLLSLKDCINLAISQNQDLKISNKQL
                     QMAEEGIRSTYSAVLPSVSFDYSATKSTVGAGERFYQGVYIPTKDTSSNYFSTGLSLN
                     QNLYNGGKRRNQIKLAKGEAESSRLNLNRTQQAIIANVTEKFYTVLKAKELLKVYEMT
                     LKNSQEQLKKTEEMYKIGQVAKKDILKAQVSEGESRLNLIKQQTSLKNALNDLKMAIG
                     LASDYALDVYEDSYLKPEIIDQAVAEQKALENNPELKSLQIQKSNSYLNYKIAQGDLW
                     PSVNGSFSYGRDGSEIERTYSEFDKWWRTSLNLNISYPLFTGFNRKANIQQKKINYNL
                     YDDQIEKKKLEIISEVERLILTINTNLEMIAINEINILSAQEDLRLAQEMYRLNSATL
                     LDVLDAQVAFTKAQGNLVTTKYDAKIAEVQLAYLMGILQQPE"
     gene            11556..12833
                     /locus_tag="PKX09_06460"
     CDS             11556..12833
                     /locus_tag="PKX09_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU17498.1"
                     /translation="MKKGGKKILITVIVIIVIGAIVLLNMNKNKEKPLAVQTEKVKRQ
                     TIVQTVNASGSLQPVSQVKISANVSARIINITVEEGQTVKKGDLLVELDRTQYEAAYE
                     KAVSTVQSAKANRNKVASELKRIKALYERNLAPEADMEAAEAQMELAESQVVQAEAAL
                     KQALDDLDKTRITAPMAGIITSVKKEVGEIALGSVFQEDVILVVSDMSKVQVEVDVDE
                     TDVVNVSIGDTALIELDAIPNTKFKGVVSEIAHSATTTGVGTQEQVTNFLVKIAVLGQ
                     DARFRPGMSATVDIITDVKEDAIAVPIQALTARPPLVKPPHGEQSPTEKTEKSQYPTP
                     TAVPFGQIKTEEVVFVVKSPVGDTTKTNKPLKVSTGPMAEQRAVKIGISSDTHFEILE
                     GLDEGEEIVVGPYKVISKDIKDGSILKITNKIG"
     gene            12849..13766
                     /locus_tag="PKX09_06465"
     CDS             12849..13766
                     /locus_tag="PKX09_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17499.1"
                     /translation="MKKLFILMFGLAFTTFLSAADSTNTNDPFDELKAIPTQDTLRDE
                     LSEEEEKSLDEIVEDENWKSRLPLNDWNWNWEEDDKEAYRRGQKHGYFRGAAGGWDIF
                     YLPLNLDDLNRSLNPIGVTDFDTYMILNGGGGWVFLGKSIRIGGFGAGGEVIAHGKPG
                     DIQKEVKLSISFGGFTIDKVFHPFSKTELYLGLMIGGGSNTINFYQHGTIVSWDSLIA
                     GYTPNTLTTSHTFHDYSSSLSADYFTLMPTVGFRYNIFRWFAVGGNVGYIYTHIDQKG
                     WRMNHYRVEGVPDLNLDNVIYRLNFYFGG"
     gene            13766..14449
                     /locus_tag="PKX09_06470"
     CDS             13766..14449
                     /locus_tag="PKX09_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU17500.1"
                     /translation="MDSLIELKDIVKIYKVGSEEVHALRGVNLSIQPNEYVSIMGPSG
                     SGKSTLMNIIGCLDTPTSGRYELEGVPVHEMDDNQLAEIRNRKIGFVFQTFNLLPRVN
                     ALHNVELPLIYSGIPAAKRKILAEAAIEKVGLSDRKNHKPNELSGGQRQRVAIARALV
                     NNPSIILADEPTGNLDSATGEEIMQIFKDLHEAGNTVVIITHEKDIAERAKRIVHIRD
                     GNIAEDIRN"
     gene            14454..15683
                     /locus_tag="PKX09_06475"
     CDS             14454..15683
                     /locus_tag="PKX09_06475"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU17501.1"
                     /translation="MISFFIKLWENVKIALRALMQSKMRTILTTLGIVIGVLTVVSVA
                     SIIEGLNRGFASQIANLGSNALYVERYPWATTEDFSKFRNRPRLTLKEADFIRERMRL
                     AEAVSPSTSTRQDIRYRNRTLNGISVNGVSDHYDVVTGLVIEEGRFFSFAEIERNKNN
                     VVLGWEIKDKLFENRNPIGEKIKIGATTYTVIGVTEKRGSFLGESLDAEVYVPLGTVF
                     KNFGWHRSISIAVKISDPARVEEAKDELRFLMRLARKLTPTQEDNFSINQQEVLTDMY
                     NRLTRGLYTAAIGIGTLSLLVGGIGIMNIMLVSVTERRKEIGIRMALGAKRKVITFQF
                     LVESVIICGVGGIIAIILSFIVSLIISKITPFPSAVPAWSVFMGLGFSAVVGLFFGIY
                     PASKAARLNPIECLRYE"
     gene            15797..17020
                     /locus_tag="PKX09_06480"
     CDS             15797..17020
                     /locus_tag="PKX09_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU17502.1"
                     /translation="MNFDEGLKLAFQTVKSNKMRTFLTTLGIVIGVTAVIGMMSIINA
                     LDRYMTKSLSSIGGNVFWIQKYPAVQVGHLDQKYRMRKDLKYEHAEAIKRSATLVSAV
                     SAEFFRWGKTVKYKDRATNPNVLVYGGDEYWADVNGRNISEGRMISLMDVQHRRAVAV
                     LGADVAEKLFPFTYPIGEQVKIDGIRYEVIGVMEKMGQAMMGNQDNIVTIPNSTFIKT
                     YGDWGSINIAVKAKSAETILDAMDQVTAILRIARKVPPGDENDFEVVTADSIMNTLRN
                     LTGFIFIAAVIICGISLVVGGIGIMNIMLVSVTERTREIGIRKAVGAKRRHILTQFMT
                     EAVGICLFGGLIGILLGVVVGLLIAQAFKLPPVIPLWSIFVGMGFSLLIGVVFGTYPA
                     LKAAKLDPIEALRYE"
     gene            complement(17635..18855)
                     /locus_tag="PKX09_06485"
     CDS             complement(17635..18855)
                     /locus_tag="PKX09_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU17503.1"
                     /translation="MKRIKIIGLFMLMATTICAADELTLEQCIEAAVANNKSLVKNEL
                     GVQQTAIGTKTAYGNLLPSVSASASASGGDGSSRSSASLHINQKIYSPGLFSDIKLAR
                     NETDIARLNYNELASQIRSEVETAYYNILTAQSLLNVYRENLTAADENLQLIQTMYKL
                     GTKTESDVLKAEVQKAEIENELISQELEIIKQKRALAILMGISPNQDYEIAKVNVEQI
                     DIPSLTEAKELLFMNNYEYQSLLKSLKSEQISLQIARESYLPSLSAGYSYSKDWTGST
                     TTDGYGSWGLSLNLGLFNGFSKKLNVQKSKLSLRSAEVSLEAKEQELLATLLNYYSNF
                     ENHNKVIALQEKNLESARKDYELVTRQCELGLATMLEQTNAQVSLLSAQTNLVEAQYS
                     RKIVESKIKQLLAL"
     gene            complement(18871..20091)
                     /locus_tag="PKX09_06490"
     CDS             complement(18871..20091)
                     /locus_tag="PKX09_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU17504.1"
                     /translation="MKKIYNLLKVAIKSIFKNRMRSLLTSLGIIIGVSAVIIMVGVGR
                     GSQAAIEDNIKSLGTNLIIIFPGVSRSGGASMGAGSFNRFTMKDVERIKESATLISAI
                     SPVVRANGQVIYKSNNWYTSISGVSPDYFSITNWELESGEFFTERDDLARKKVALLGK
                     TVATELFGADNPVGEQIRIRNVPFTVIGVLKEKGGSGPGGDQDDIILAPSTTVLYRLK
                     GSQWIDMINASAISEEAMTAAQEEIRTILREAHRLGPADGDDFNIRTQSEIIDFASST
                     SKTMTLLLGAVACVSLVVGGIGIMNIMLVSVTERTREIGIRMSVGARASDILSQFLSE
                     AVVLSLSGGIIGIILALAASALLNNLTDLRTVIDPVIVILAFTFSGAVGIFFGWYPAR
                     KAANLNPIDALRYE"
     gene            complement(20088..20843)
                     /locus_tag="PKX09_06495"
     CDS             complement(20088..20843)
                     /locus_tag="PKX09_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU17505.1"
                     /translation="MPIVIEVRQLSKTYQVGDIEVHALRKVDFAVAAGEFTAIMGASG
                     SGKSTLMNMLGCLDQPTGGEYLLDGVNVSTLTSDERADIRNQKIGFVFQGFNLLPRTT
                     ALENVELPLIYDRHQRIKNPQSAAKEALERVGLGDRMHHIPTQLSGGQQQRVAIARAL
                     VNQPSILLADEPTGNLDSKTSMEIFSLFQQLNDEGLTIVMVTHERDFATYAKRILQMR
                     DGVLHKDTLIKKRRNAAEDLKMLIAKQQQEEEQ"
     gene            complement(20890..22158)
                     /locus_tag="PKX09_06500"
     CDS             complement(20890..22158)
                     /locus_tag="PKX09_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU17506.1"
                     /translation="MKKKICLIVILVLAVAVGWHLIRKNGSDKQIQIAYGQVTRGNIE
                     TIISSTGTLVAIGTVDVGTQVSGRIDKVLVDYNDIVKKDQVLAVLDTLLLSQSVKEAE
                     ANLHKMQALYEQAAFQYDRISNLFQQKLVSEQEYIDARTNKATSEVSLKQAQIALDKA
                     VADYGYAIIRSPISGTVIDRVVEEGQTVAASFSTPTLFTIARDMTKMEIEALVDESDI
                     GEIKTDQKVRFTVQSYQDKIFNGVVRQVRLQPQTVSNVVNYTVIVDADNHEQLLLPGM
                     TATVDFIIDSRENVLIVPNSALKITPTEEMLAKAHQVRQAQMEQLPESVKTQFREQSP
                     KPQSADQSPEELDKSTRPTPIWYVNESGQILMALVKTGLSDTKNTEILENPEIKEETK
                     IIVSLIANGKNSVSNSSNNQQRGMGGPPPF"
     gene            22308..22871
                     /locus_tag="PKX09_06505"
     CDS             22308..22871
                     /locus_tag="PKX09_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU17507.1"
                     /translation="MENLASLTDQEIIDACLSGNENAFAEIIRRYEPRVAATVIGMLG
                     RCPEAEDVGQETFIRFYKSMSKFRGESSLSTYLTRIAMNLSINELNRRKRQSRFVPLD
                     DPEISRKTSGDPGNQADSESGAIIRRAVAQLAPKFRSTIVLRLIEGYSTEETAQILQL
                     PVGTVLSRLARAKVQLMNLLTPYKELL"
     gene            22868..23296
                     /locus_tag="PKX09_06510"
     CDS             22868..23296
                     /locus_tag="PKX09_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17508.1"
                     /translation="MKIDKIRKLLLLSGDRNLTAKEQKILMSARQTNPELAEDEQLLQ
                     KIQQNLAGTKQESFEPWFAERTMNRIRQLASQEKHPARLWNGFWPAYSRVIIGAAVIL
                     ALIVVFNLFTSSEPSLAKAFGITQTPIEAIMDPIAYLIWE"
     gene            23302..23715
                     /locus_tag="PKX09_06515"
     CDS             23302..23715
                     /locus_tag="PKX09_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17509.1"
                     /translation="MKKTTRTTLMLSSVLILGIIIGFVGASSWLHYNQKKRFTHFRKG
                     EGFVVEMEKMIDPLPEQRDEIHEILLRHSLWVKQFSEEQRAILQRSIDSLDAELAHVL
                     TPEQMRRFQEKVKNQPRRPGPPPGPPPPPFEEPHP"
     gene            24307..25164
                     /locus_tag="PKX09_06520"
     CDS             24307..25164
                     /locus_tag="PKX09_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17510.1"
                     /translation="MRKISKITLLEMGVLMALLSTCKKATVQSAWNDGSIIIDAVVKD
                     WGSRLVWNEKSQVNLGVTNDAENLYLCLVTSDPDIHRQILINGFIVGIDQKGSKSHHF
                     GIKFPLGIGDRGNQLLMPTDDQRRQKPFEPSQPGQKGRENDFLAKFNEQQTELILLGP
                     GDKQKTLVPLENDLGLEVKIGFDRQQLVYELKLPFSVISQHLQIDQISNNKPLTVSFK
                     TDKVEIPSGRPGDMMDSPGGMGRPEGGMGGPGGRMGVPDARMGNPGGDEHSEKTFSYQ
                     VAVTLAVKP"
     gene            complement(25375..26328)
                     /locus_tag="PKX09_06525"
     CDS             complement(25375..26328)
                     /locus_tag="PKX09_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOU17511.1"
                     /translation="MANKTIELKGVDPALVFGVRDVFINKIEESFGARIFARGTKINL
                     EGDENTLSQIERVIGEMLMTINRKGFIDEQDVTTLINMELGGQQLPGEITADTVILFK
                     KTGVIKPRSVNQEHYFHATLKNDLVFGIGPAGTGKTYLAVAIAVAALRNHDVQRLILT
                     RPAVEAGESLGFLPGDLTEKIEPYLAPLYDALYDMLPPEKVRVYEEQHIIEVLPLAYM
                     RGRTLNNAYVILDEAQNTTPMQMKMFLTRLGANSKAIITGDITQIDLPDRQKSGLIDA
                     LEILRGIEGIEFVYFDTTDVVRHRLVKKIIKAYNGESPNKS"
BASE COUNT         7355 a         5486 c         6202 g         7367 t
ORIGIN      
        1 gcgattgcta cttatacagc gcctaaggat agccgtattt atggaactta tgaggtagat
       61 gtcacctcag ttctccagta tatcaaggag aaaagggaag ccggaattca tctgactata
      121 acctattttg tagcggcggc gattgctcgg gcgctttatg aagatatgcc ggaagtaaat
      181 tgttttatcc gacggggaca ggtagtcgcc cgcgagaatg ccgatgtctt tttatcggta
      241 aatgtcggcg gggaggcgat gaccggtttg gtattgagaa aatgccagga actatcagcc
      301 actgaaattg gccagataat ccagaaagtt gttgaaaaaa agcgcgatgg caaggaagaa
      361 gaaggcgcct ttgccgccaa agataaatta gctaaaattc cctggccttt tcggcggcct
      421 gtatttctat ttataaaatg gtggattttc gatatggggt ggacatttcc atttttgaaa
      481 atcaaaccag atccgttcgg gagtattatg ttgactaata tcggaacttt tggtctgcat
      541 actggaatgc cagcgctttt tccaattggt aaacttccgg cagttgtcgc gatggggaaa
      601 atagcgaaaa agccggtggt gattgatgat cagattgtca ttagagacat tttaccgctg
      661 actgggactt tcgatcatcg gattgtggac ggttaccagg ctggggtatt aaccaaaggt
      721 gtcgagaggc gtctgcaaga tccggaggct ctaaatcgcc cgaataattc aaacaattct
      781 aactgaataa tttttacaaa ggagattaaa aatgtctgaa atcgaaagca aaatcctgtc
      841 tgccatcgaa aaggcaggta agccactgaa agccggggag gtagccgaaa tgaccggttt
      901 aaccaaagac gaagtcgcca aggttatcgc gggcttaaag aaacagggaa aagtcgaatc
      961 gcccaaagcg tgctactacc aggtgaagaa aagttgacgg atagccgata agagccaatc
     1021 agctaactcg gcctggattt tattccaatt aatatttata tcgtgagaat agaaagtatt
     1081 atactgaatg ccgaatggca tctgatagat aactcaactg atgattttaa aatgcattaa
     1141 ttaattggag gaagagaaat ggatttatgg aattttacat tgaattttaa ggatatagtc
     1201 cttgattttt cttttatgag tgtattttta gttgccggga cagtttttcg gcgctacggt
     1261 ctttttttcc agaaatattt ggtgccgaat aacctgattg ccggttttat cgggttaatt
     1321 ctgggctcgc aagtattgaa cattgtgcca atctccgaag aaagaatggg gctgtacgtt
     1381 tatcatttgt tggcgctgac tttcgttgct attggtttgc gcggtgaaaa aacttcctgg
     1441 ggtaaaggcc ctttaagttt ttccttgagt atgctttcga gttacatcat tcagggatta
     1501 atcggtatgt tggtatgctt tgggttcgta tatactattc gtcccgacct ttttataggg
     1561 cttggactgc tattgccgat gggatttgga atgggaccgg gacaggcctt tacaataggt
     1621 aaaggttggg aaaagtacgg tttcaccggc ggcggaatgg ctggtttaac gattgcggct
     1681 atcggatatt tagtcgctta ttttgccggt atggtcattg tcaataaagg tatcaagcgg
     1741 ggcgagacgg cactaatcca agggatggac tcgattactt ctgatatgcg caccggcgtt
     1801 attaaaaagg gtgaaaaacc gattgctggt tttttgacct tatcaatgga agcgattgag
     1861 ccgctggctt ttcaggtggc ggtaattggg gttgtatatt taatatcgtt ttgggtggct
     1921 aaaggggtgg aaattgcatt gcttaaaatt ggagccggcg gattcgttgc aaccgtttgg
     1981 gcttttcatt ttgtaattgc cctcttggtg gcgctggcga tgcgtaagtt tttagataaa
     2041 atcaaaaaaa gctacttgat tgatccgggg cttatgactc gcacggctgg gcttttcgtt
     2101 gattacctga ttgtcggttc gatttgtggg atcagcctga aagttatctg gatgtactgg
     2161 gcgccgattt tagttatgtc ggtcattgga ggctttgcta cttattattt actgaagtat
     2221 gtttgttatc gcgcgtttga cgattaccat tttgaacggt ttgcggcgat ttttgccgaa
     2281 atgaccggga cgctaaactc cggtttagta atgattcggg taaccgatcc ggaattcaag
     2341 acgccggtag ccgaagactt ggtctattcc agcggcattg cgctatttct ggggttgcca
     2401 cttttaatac tgctgaatgt gccgatgaat tttttcaata atagcctcaa aggatactgg
     2461 attacattcg gtcttttaat agtatatggc gtgattttgt ggatattctg gcgggcaatc
     2521 ggttttattc attttaagaa aaatcctaaa tccaaagttt aataagattg ttgaaaattt
     2581 acttaattta tgttaaaatt gaatccgatt tgatgatctg ttttagttga aacaatgtaa
     2641 ggagcattaa ttgaaaaaga gaatcggaat attgacaggt ggcggcgatg tacctggttt
     2701 aaatccggca attcgcggca ttactttaag ggcattaaaa gagggctttg aggttattgg
     2761 tatccgacgc ggctgggcgg ggatgatcaa tatcattcga gataaagagg ctgacaacag
     2821 ccagaatttc atcatgctga atcaggaaat cgtcaacaaa gccgggcgca ccggtgggac
     2881 atttttacat acttcccgca cgcggccaag tagtgttaaa ttggatgccg tgccggagca
     2941 tttgcgggat aaatatacag ctgaggttaa tgatctgacc cctgaagtac ttaaaaacct
     3001 cgattttctc gggattgatt acctaattcc gatcggcgga gatgatactc tgagctacgg
     3061 cgtcactttg cataaagcgg gggtcaaggt gattggtgtt cccaaaacta tggataatga
     3121 tgtccccggt acggattatt gcatcggttt cagtacttgt gtgacgcgga cgctggagtt
     3181 gactcatagt ttgcggactt cagccggttc acacgaacgt tttctggtaa tcgaagtttt
     3241 cggacgctat gccggtttta cggctttgct tccgactcta gctggggctg ccaaccgctg
     3301 tgtaattccc gagtataaat tcaatatgga gcgcttaacc gagctattgg tgcaggatcg
     3361 ctttactaat cccagtcgct attcggtggt tctagtttca gaaggtgcta tgatgcagaa
     3421 tcaggatcag atgatgttcc agagcgaaga gactgatgcc tttggtcata aaaaactggg
     3481 cggaatcggg gatgtagttg caaggcagct taaagaacta tccggaaaat ataataacgg
     3541 tcataaaatt aacgtcatca gccagaaact cggttatttg gtgcgatgcg gtgatccaga
     3601 tgccctcgat tcgatcgtgc cgatggctta tggtaatatc gcatttgagt tgattatgaa
     3661 tggtcgttcc ggactactgg tcaacttgcg taatggtgtc tatggtcata cggacctgga
     3721 ggttgttact agcaccaaaa aagtggtaga tgttgaaaaa ttttatgaaa ccgatcgcct
     3781 gcgtcctaag tataagagtt ttgaaaacaa atcgtttttt atcatgacca gcgataagta
     3841 aacaaagttt ccctcctccc tcctctctat gaggacaccg caaaagggca atcgcatgat
     3901 tgcccttttt gctttaaaaa ggagcggcaa ggtgatattt tttgggcttc acttttttat
     3961 tggaaccata atttatcctt taactgattt atggtgttga agctgattat gatgatgaaa
     4021 tggatagcat taggttaaga agggtcaatc tttcctccct ttttaagact aattgaaaat
     4081 attcccttca tttttttccg aaaaaatacg tgtggctctg caattacctc ccttatagag
     4141 atagcggttg cttttctggg catttcggtc gtaactttct ttagttttga aagtgatgaa
     4201 tttactaatt cagaaaaacc cggcaataat tcaaataatt ttataaacat taaaatattc
     4261 aatggagaat caccatgtct aaaactatga aaactatgga tggcaataca gcggcaactc
     4321 atatagccta tgctcttagt gaagttgcgg ctatttatcc gattacacca tcgtctgtga
     4381 tgggagaact ggctgacgaa tggtcggcgc aaggtcggaa aaatattttc ggacagcccc
     4441 tgcgagtggt tgaaatgcag agtgaagccg gtgcggcggg agccgtgcac ggcagtcttt
     4501 cagctggcgc gctgaccaca acctttaccg cttcgcaggg tcttctatta atgattccta
     4561 atatgtttaa gattgctggt gaattgatgc cagccgtatt tcatgtttcg gcgcgagcgg
     4621 tagccgggca agcactctcg atttacggtg atcattcgga cgttatggct actcgcacga
     4681 ccggttttgg tttaatggct tccggatcgg tgcaggaagt gatggattta tcactggtta
     4741 ctcatttagc ttcgctgaaa gccagcctgc cgtttgtcag tttcttcgat ggttttcgca
     4801 ccagccacga aattcagaaa atcgaagtca ttgattacga gatgattaag tccttagttg
     4861 attttaccga tattgatcga ttccgagcgc gctgtttgaa cccggaacac ccgcaactcc
     4921 gtggcacggc tcagaatccg gacatctttt ttcaagtagc tgaattggct aataaatatt
     4981 acgatgccgt tcccgaaatc gtcgctgaaa tgatgcggaa agttggggaa gttaccggcc
     5041 gtcattataa cctattcgat tatgccggtg atccgaatgc ggagcgagtg gttgttataa
     5101 tgggttccgg gtctgagact gttcagggcg ttgctgaata cttaaatgcc agaggcgaga
     5161 aagtcggcgt attaaaggtt cacctgtatc gtcccttctc ggctaagcat tttatggcgg
     5221 ctttgcctaa gagtgctaag cggattgctg tgctggatcg taccaaagaa tccggcgctt
     5281 ttggtgaacc gctttatacc gatgtatgtg ctgttttaca gcgagaaaaa gataatcgct
     5341 tagtcgttgg cgggcgctac ggcgttggcc aaaaagaatt cagtccggcg atggcaaaag
     5401 ccgttttcga caatctgaaa ctgcccgagc cgaaaaacgg ttttacggtg ggcatcgaag
     5461 acgatgtaac cttccgcagt ttgccggtca ccgaagatat tgatactgtg ccggaaggta
     5521 ccgtccaatc tattttctgg ggttttggtt cggatggaac ggtcggtgcc aataaagatg
     5581 caattaaaat catcggcgac aataccgatt tatacgcgca gggctatttc tcttacgatt
     5641 cgaaaaaatc gggtggtttg acggtcagct atttgcgttt cggaccaaaa gaattacata
     5701 caccctatcg gattaatctg gcagattatg ttgcctgtca taaacagagt tatatcaata
     5761 attacgatat gctgattaat cttaaaccgg gtggcatttt cgtactgaat acgctttggg
     5821 atgacgccga gctggacgct aatttgccag ccgctatgaa acgggcgatc gcaaccaaga
     5881 aagttcggtt ctatgcggta gatgccaata gaattgccgg tgaagtggga ctcggaggtc
     5941 gcatcaatat ggtaatgcag acagccttct tcgcgctgtc caaagttttg cctttcgagg
     6001 aagccatcaa gcacttaaaa aatgctattc agaaaactta cggtgccaag ggtgaaaaaa
     6061 tcgtcaagat gaactggcag gctgtagatt ctgccatcaa tgccattcgt gaagtaaaat
     6121 acccagcgtc ttgggcggaa gccaaagaga cggccgaatc gccaaaacct gtaccggatt
     6181 ttgttaaaaa agttttacag ccgataaatg cctttaatgg tgacaatttg ccaagtagcg
     6241 tattcacgcc gggtggcatt ttcccgacgg caacaaccaa atatgaaaag cgcggaatcg
     6301 ccatcgaagt tccagaatgg atcaaggaaa attgtaccca atgcaatcag tgcagttttg
     6361 tctgcccaca tgctgtaatt cgtccggtac tggtcactcc ggaagaaaaa gccaaggcgc
     6421 ctacaacttt cgagaccatt aaagccatag gcaaaggcct cgaaaattac gagtatcgta
     6481 tgcaggtctc ggtgctggat tgcaccggtt gcggtaactg tgccgacgtc tgcccggggc
     6541 gtaaaaatga aaaggccttg gtgatgaagt cgtttgagtc tcagctggaa cagaaagaga
     6601 actgggaata ttttgaaacc attccagtca aggctaatgt aatgcggaaa gataccatca
     6661 agggaagtca gttccagcaa ccgctttttg aattttccgg tgcctgtgga ggatgcggtg
     6721 aaacacctta cctcaaagtc attactcaat tattcggcga ccgtatgatt atcgcgaacg
     6781 ccaccggttg ttcgtcaatt tatagcggta gcgtggtttg cccttattgt gtcaatgaaa
     6841 caggatatgg accagcctgg gctaattccc tctttgaaga caatgccgaa tatggtttcg
     6901 gaatggcttt agccattatt cagcgtcgtg aatatctggc ggaaattatt aatgaggcac
     6961 tcaaatcagc cgatggagaa ctggcggaag cttttaaagc ctggcttgat ggcaaagacg
     7021 atgccgaact ctcgcgcgcg gcgggcgatc gcatcaaagc cgctctggct gataaatggg
     7081 aaggtaataa gctattgacc gagatctgga aaaatcgtga tattttgact aagaaatcaa
     7141 tctggattat tggcggtgat ggttgggctt atgatatcgg ctacggtggt ctcgatcacg
     7201 ttattgctat gggatatgat gtcaatattt tggtgctcga tacagaagtt tattctaata
     7261 ccggcgggca gtcgtccaaa gcaacgcctt taggttcagt cgccaaattt gcttactccg
     7321 gtaaaaagac ttccaaaaaa gaccttggta tgttagccat taattataaa aatgtctatg
     7381 tggcttcggt ggcgatgggc gccagcgcca accaattctt aaaatccgcg ctggaagccg
     7441 agaaatatcc gggtccttct ttgataattg cttattgtcc ctgcatcaat catggtattt
     7501 acaagggaat gctctcccag gaagaatcca gacaagccgt cgagtgcggt tactggccgc
     7561 tttaccgtta taatccttta ctaaaagccg aaggtaaaaa tccgttccag ctcgaaagca
     7621 aggaacccaa tggtaagcta atggaatttt tagatggcga agtgcgttat gccaccctca
     7681 agaaatcttt cccagaggaa tcggaaaagc ttagaaaagc cttgtctgaa gaagtaatag
     7741 agcgctatca aaaatataaa cgaatggcgg aagaaagcta agtttagaat aactgtgatg
     7801 atgaaaaaag ggcgctaggc aatcaagttc tcttaagtgc ttctatgtgc ttataagaaa
     7861 ttaatatact tctcttaggc ttcagtagtt caatacttat tcagtcaacc acgcactcat
     7921 gttatcgaaa ttagtccggc tttgcggaac attttctctt caattaatat tgtgattaac
     7981 tgaccaggtt ggtaaattga cgagcatttt aaaagtttta ttatgacatt tcttaatttt
     8041 ttattccctc cagatgatcc tctgtctgat attttaaaga aaatctccac cgtggctgag
     8101 tgtaatcata agcgcgtctt tttggtcggc ggtgtggtgc gtgaccggtt attgggaatt
     8161 gatactaaag acattgatat ttccgttatc ggtaatggga tagaatttgc gcacaaactg
     8221 gcaaaaagtc tgaaagttga caatgttatt gaatacccac gctttggcac tgctatgata
     8281 ccctatcatg ataaattgat cgaagttgct acggctcgga tggaggaata cgaaacctcg
     8341 tcgcgcaaac ctcagattat cgaaagtagt ttacgtgacg atttagcccg ccgcgacttt
     8401 acgatcaatg cgatggcact ttccttgaat cgggaaaatt acctagagtt gacggattat
     8461 ttcgaaggtc taaaagacct caatgccgga attattcgta ctccgctgga cccgattacc
     8521 actttttccg aagacccgct gagaatgctg agagccgttc gtttcgccac tcagttgaac
     8581 tttcggatcg agcccaatac tttcaaagct atagagcagg tccgtgagcg aatcaaaatt
     8641 atttcgcagg aacgtatcac cgatgaagtt accaaaattt tgatggtgcc aggaaacccc
     8701 tcgcgcggtt ttgaactact gcaggaatct accctgctgg atttgattct accggaaatc
     8761 gcggctcttg atggagttga tcaacgcaac ggctatcatc ataaagatgt ttttaagcat
     8821 actttacagg tgctggacaa tgttgcccgg gtaagtacca aatttgaatt gcgtctaacg
     8881 gctcttttgc atgacgtagc caagcctttg accaagcaat ttttcccgga caaaggctgg
     8941 acattctacg gacacgagga attgggcgcg caaatggttg gtgaaatcgg gaaacgttta
     9001 cgattaccga acaaaacaat cgaatacgcc gccaaactga ctcgtttaca tttacggccg
     9061 atcgccattg cttcggaagg aattaccgac agtgccgtgc gacgcctcat cgtcgaagcc
     9121 ggagatgaat tagatgatct gatattgcta tgtcgggcag acattacctc aaaaaatatt
     9181 caaaaagttg aagtttatcg cgcgaatttc gaccgagttg tcaatctaat cgagagcgtg
     9241 cgggaaaaag accgtctgcg ggctttccaa tcgccggtac gcggagagga aattatgcgg
     9301 ttctgtaatc tgccacccgg gccggcggtc ggatttatca aatcggcgat cgaagaggca
     9361 attttggagg gcgagattga caatacctat gacgccgctc acacatttct ggagaaaaat
     9421 tgggaaaaat tgcttaaaaa gttcaactta aagccgaaag cctgacaacc tttagaatgt
     9481 tcatttatct aaaacacgag tttttatcac caatggagta gaaattaaaa tgagcgatga
     9541 cgagaataag actatttttg gtaaaataat ttctattttc tgggaaccat cggcaacctt
     9601 ccgggctctg gtgatcaaag ttagctggct ggatattgtc gtgccgcttc tgctggtcct
     9661 gctggtagcg ctggccgtga tgccatatat aacgccgctg gcggttgagg agcagaaaga
     9721 ctatatcgcc cagagtgatc gtctgactga agaacaaaaa gcccaggctt acgaccgaat
     9781 agatcagcag gctggctcgg ctcgcgctta tattactagt gccgtcttcc tggtgatccg
     9841 aaccgccgtg gtggctggag ttttgatttt ggttagcaat tttctattcg gcggcgaagt
     9901 taagtataaa agtatgctag cagtgagtgc ctatgccaat ctggtggaca ttatcgctaa
     9961 tgcggtaaaa gttccactta tgttatctca gcagactgtg cggatttata ccagcccggc
    10021 tttatttctc caggacaact ccactttttg gtttaggttt ttcgccaatc ttgacctttt
    10081 tgttctctgg aaagtgattt tactgggtat cggtgtggga ataataacta aggtgaaaac
    10141 cagtaagagt atctggacca tcctcatttg ctggatcatt tattgtctgg cggttgccgg
    10201 tctgggcggc ttagccaaag tataacatag gttttaggtt ggtagctatg aaaagagtaa
    10261 tatttatttt attaattggg caaattctgg catacggtgc cgatttattg tcattaaagg
    10321 attgcataaa tttggcaata tctcaaaatc aggacttaaa aataagtaat aaacaattgc
    10381 agatggcgga agaaggtatc cgcagtacct atagcgctgt tttaccatct gtcagttttg
    10441 attattcggc gactaaatcc actgtgggag caggtgaacg attttaccaa ggcgtttaca
    10501 ttccaactaa agacacgtct tccaattatt tcagcacggg tctttctctc aatcagaatc
    10561 tttacaatgg cggcaaacga cggaatcaga taaaactcgc caaaggggaa gcggagagtt
    10621 ctcgattgaa tctcaatcgc actcaacaag ccattattgc caatgtcacc gaaaaattct
    10681 atacagtttt aaaggctaaa gaattattga aagtctatga aatgactttg aaaaacagcc
    10741 aggaacaatt gaaaaaaacg gaagaaatgt acaaaatcgg acaggttgcc aaaaaagaca
    10801 tactcaaagc ccaggtcagc gaaggtgaga gccgtctgaa tttgattaaa cagcaaacct
    10861 cattgaaaaa tgctttgaac gatttaaaaa tggcaattgg tctcgcgtct gattatgctc
    10921 tggatgttta cgaagattca tacctcaagc ctgagataat tgaccaggct gtagccgagc
    10981 aaaaagcgct ggaaaataat cccgagttga aatccctcca aatccagaaa tcgaactcct
    11041 atttgaatta taaaatcgcg caaggcgatc tctggccgtc ggtaaacggc tctttttctt
    11101 atgggcgaga tggttcggaa atcgagcgta cttacagtga attcgataaa tggtggagaa
    11161 cttcgcttaa cctgaatatc agttacccac tttttaccgg ctttaatcga aaagcgaaca
    11221 tccagcaaaa gaaaatcaac tataatcttt atgatgacca gatagaaaag aaaaaactgg
    11281 aaattatcag tgaagttgag aggctaattc tgaccattaa taccaatctg gaaatgatcg
    11341 caatcaacga gatcaatatc ctatcggcgc aggaggattt gcgtttggcg caggagatgt
    11401 accgtttaaa ttcggctacg cttctggatg tgctggatgc ccaggttgct ttcaccaaag
    11461 ctcagggaaa tttggtcacg actaaatatg atgccaaaat cgctgaagtc cagctggcat
    11521 atttaatggg aatattgcaa caaccggagt gaattatgaa aaaaggcgga aagaaaattt
    11581 taataacagt aatagtaatt atcgtcattg gtgcaatcgt tttgctcaat atgaataaga
    11641 ataaggaaaa accactggcg gttcaaacgg aaaaagtcaa gcgtcaaacc atcgttcaga
    11701 ctgtcaacgc ctccggttct ttacagccgg tttcgcaggt aaagattagc gcaaatgtca
    11761 gtgcccgaat aattaatatt acagttgaag aagggcagac ggtaaagaaa ggcgatttgc
    11821 tggtggaact cgatcggact caatacgaag ccgcctacga aaaggcggtc tcgaccgttc
    11881 aatcagctaa agccaatcgc aacaaagttg ccagtgaact aaagcgcatc aaggctctct
    11941 atgaaaggaa tttagcgccg gaagcagata tggaagcggc tgaagctcag atggaattgg
    12001 cggagagcca ggttgtccag gcggaagccg ctctgaagca ggcactggac gatctcgaca
    12061 aaacccgtat cacagctcca atggctggaa tcattaccag cgtgaaaaaa gaagtcggcg
    12121 aaatcgccct cggctcggtc tttcaggaag acgtaatcct cgtcgtttcg gatatgtcca
    12181 aagttcaggt ggaagtggat gtggatgaaa ctgacgttgt caatgtatca atcggcgata
    12241 cggcactaat cgaactggat gctatcccca atacgaaatt taagggagtc gtatcggaaa
    12301 ttgcccactc cgccactacg accggagtcg gtacccagga gcaagtaacc aattttctgg
    12361 ttaagattgc cgttctcggt caagatgctc gcttccgacc cggtatgtcg gccaccgtgg
    12421 atattattac cgatgtaaaa gaagatgcga ttgcagttcc gatccaagcg cttacggcgc
    12481 gaccaccgct tgtaaaaccg ccacatggcg aacaatcccc aactgaaaaa acagaaaaat
    12541 cacaatatcc gacaccgact gcggttccct tcggacaaat caagactgag gaagtcgtgt
    12601 tcgtcgtgaa atctccagtc ggagatacaa ccaagactaa taaaccttta aaagtatcaa
    12661 ccggcccaat ggctgaacaa cgcgctgtta aaatcggcat tagctccgac acccattttg
    12721 aaatcctcga aggtcttgat gaaggcgagg aaatcgttgt cggtccctat aaagtcatca
    12781 gcaaagatat caaagacggt tcgatattaa aaatcacaaa caaaatagga taaagaagga
    12841 gcaaagcaat gaaaaagtta ttcattctaa tgtttggatt ggcatttact acgtttttaa
    12901 gcgcggctga ttcgaccaat accaatgatc cctttgatga attgaaagca attcccaccc
    12961 aggataccct ccgcgatgaa ctttctgaag aagaagaaaa atcgctggat gaaatcgtcg
    13021 aggatgaaaa ttggaaaagt cgtctcccgc tcaacgattg gaattggaat tgggaagagg
    13081 atgacaaaga agcctataga aggggtcaga aacacggcta tttccgtggc gcggctggcg
    13141 gttgggatat tttttacctg ccgttgaatt tagatgattt gaatcgctct cttaacccga
    13201 tcggcgtcac tgactttgat acatatatga ttctgaacgg tggtggcggc tgggtatttt
    13261 taggaaaaag tatccgaatt gggggatttg gtgccggagg agaggtgatt gcccatggca
    13321 aaccgggtga tattcagaaa gaagttaaac tttcaattag ttttggaggt tttacaattg
    13381 acaaagtttt tcaccccttt agtaaaacgg agctgtatct cggtttgatg attggtggtg
    13441 gcagtaatac cataaacttt tatcaacatg gaacgattgt ttcttgggat agtctaattg
    13501 ccggctatac tcccaatact ctaacgacat ctcatacttt tcatgactat agcagttctc
    13561 tttcagccga ttatttcacc cttatgccta cggtaggttt tcgctataat atcttccgct
    13621 ggtttgcagt aggtggaaat gtcggatata tctacaccca tatagatcaa aaaggttggc
    13681 ggatgaatca ctatcgcgtt gaaggtgtgc cggatctgaa ccttgataat gtaatatatc
    13741 gtttaaattt ctatttcggt ggctaatgga ttctttaatt gaattaaaag atattgttaa
    13801 aatatataaa gtcggtagcg aagaggttca tgcgttacgc ggtgtgaatc tctcaatcca
    13861 accgaacgaa tatgtctcga ttatgggacc atccggttcc ggtaaatcca ccttgatgaa
    13921 tattatcggc tgtctggata ctccgacctc cggtcgctac gaattagaag gcgtaccagt
    13981 tcacgaaatg gacgacaatc agctggctga aatccgtaat cgcaaaatcg gcttcgtgtt
    14041 tcagactttt aatttactac cgcgggtaaa cgccctccat aacgtggaac tgccattgat
    14101 ttacagtggc ataccggcgg ctaaaaggaa aatattagcc gaagccgcca ttgagaaagt
    14161 tggtctttct gatcgcaaaa atcacaaacc gaatgaacta tccggcggtc aacgtcagcg
    14221 cgtggcaatc gctcgagcat tggttaataa tccgtctatt attcttgccg atgaaccaac
    14281 tggcaatctg gattcggcca ccggcgaaga aatcatgcag atttttaagg atttgcatga
    14341 ggctggcaat accgtcgtaa tcataactca cgaaaaagac attgccgaaa gagccaagcg
    14401 gattgtccat attcgcgacg gcaatatcgc tgaagatatt cggaattgaa aacgtgattt
    14461 ccttttttat taaactctgg gaaaatgtca aaattgcttt gcgtgcgctt atgcagagca
    14521 aaatgcgcac tattctgacg acactcggaa tcgtaatcgg tgttctgacg gtcgtttcgg
    14581 tggcttcgat cattgaagga ttgaatcgcg gatttgccag tcaaatcgcc aatcttggta
    14641 gcaatgctct ttatgtcgag agatatccct gggcgactac ggaagatttt tccaaattcc
    14701 gcaatcgccc gaggctgaca ttaaaggaag ccgattttat tcgggaacgg atgcgattag
    14761 cggaagcggt ttcgccttct accagtacgc gtcaggatat tcgttatcgc aatagaaccc
    14821 taaatggcat ttcagtcaat ggcgtctcgg accattatga tgtggtcacg ggacttgtga
    14881 ttgaggaggg gcgttttttc tcttttgccg aaatcgaacg taacaaaaat aatgtcgttt
    14941 taggctggga gatcaaagat aaattattcg aaaatcgtaa ccccattggc gaaaagatta
    15001 aaatcggcgc aacgacctat accgtgatcg gtgtgaccga gaaacgcggt agtttcttag
    15061 gcgaaagtct cgatgccgag gtttatgtcc cgctgggcac cgtttttaag aactttggct
    15121 ggcaccgtag tatttcaatt gcggtaaaga taagtgatcc tgccagagtc gaagaagcca
    15181 aagacgaatt gcgttttcta atgcgccttg cccgcaaatt gacaccaacc caggaagata
    15241 atttttcgat caatcaacag gaagtgctga ctgatatgta caatagattg actcgtggac
    15301 tttataccgc cgcgatcggg atcgggacgc tgtcactact cgtcggcgga atcggcatta
    15361 tgaatattat gctggtttca gtcacggaac gtcgcaaaga aatcggtatc cgaatggcgc
    15421 tcggcgccaa gcgcaaagtc attaccttcc aatttctggt cgaatcagta atcatttgcg
    15481 gcgtcggcgg tatcatcgct attattttga gttttatcgt ctcattaatc attagcaaaa
    15541 tcacaccgtt tccctcggct gttccagcct ggtcggtttt tatgggctta ggattttcag
    15601 cggtagtcgg actatttttc ggaatttacc cagcgagcaa agccgcccgc ctcaatccaa
    15661 tcgaatgctt gagatatgaa taaaatatga aaaccgtaat taccaaattt ttaaatgaat
    15721 gactatcgaa cgaccactga atgatcacaa acgaccacca aattaaagcc acctttattc
    15781 ctggaaaaag gtcaagatga attttgacga aggtttaaaa ctcgcttttc agacagtaaa
    15841 gtccaataag atgcgcacgt tcttgacgac gctgggaatc gttatcggcg ttacggccgt
    15901 tattgggatg atgtccatta taaatgctct ggaccgctat atgactaaga gtctaagtag
    15961 cattgggggt aatgttttct ggattcaaaa atatccggcc gtacaggtgg gacatctcga
    16021 tcaaaaatat cgcatgcgta aggacttgaa atacgaacac gccgaagcca ttaaacgcag
    16081 tgctaccctg gtttcggcgg tttcagcgga atttttccgc tggggcaaaa ccgtcaagta
    16141 taaagacaga gcaactaatc ccaacgtgtt agtttacggc ggtgacgagt attgggcgga
    16201 tgtcaacggg cgcaatatca gcgaaggacg aatgataagc ctgatggatg tgcaacatcg
    16261 tcgggcagta gctgtattag gagctgatgt agcggaaaaa ttatttccat ttacctatcc
    16321 aatcggcgag caagtcaaaa ttgacggcat tcgctatgaa gtcatcggag tgatggagaa
    16381 aatgggtcag gcgatgatgg gtaaccagga caacatcgtc acaatcccga actcgacctt
    16441 tatcaaaacc tacggtgact ggggttccat taatattgcc gttaaagcta aaagtgcaga
    16501 gacgattctc gatgcaatgg atcaggtgac ggcgatatta cggattgcgc gcaaagttcc
    16561 gcccggagac gagaatgatt tcgaggtggt aacagctgac tcgattatga ataccctgcg
    16621 taatctaacc ggttttatat tcattgcggc ggtgattatc tgcggaattt cattggtagt
    16681 cggcgggatc ggcattatga atattatgct ggtttcggtg acagaacgta cccgggaaat
    16741 cggcattcgc aaggcggttg gtgccaagcg acgtcatatt cttactcaat ttatgactga
    16801 ggcggttggc atttgcctgt ttggtggctt gatcggaatt ctattaggag tggtagtggg
    16861 attgttaatt gcccaggcat ttaaattgcc accggtaatt cctctctggt caatctttgt
    16921 cggaatgggt ttctcgcttt tgattggggt ggttttcggg acttatccgg cgctgaaagc
    16981 cgctaaactc gatccgatcg aagcactcag gtacgagtaa agaggctgag gctgaggctg
    17041 agaatagtca ttagtcatta gtcattggtc attagtcatt ggtcattggt aaagaggctg
    17101 agtaaataga aaagagattg aggattggag cagaggagca ttcaatctaa ccatccaatt
    17161 taccattacg gccttaagaa catctccggc tggggagcga attcccagcg gaataaatgg
    17221 aattttcagc gctgtcatag cactttcgta aatatctatt accttttcct atgtcactta
    17281 ccttatatga acttgataac ttttgcgcct taaattccat tcgctgggac tgaacgaacc
    17341 cccgtgagat gttctttagc cgttgccttt tttggttctt ttttcggcaa aggaaaaaag
    17401 aacaggaggg ttggtcattg gtcattagtc actggtcatt ggtcattagt caagagactg
    17461 agtaaagaga aagagtaaag aggttgaggt tgagagagaa ttcaaatctt agcctttttt
    17521 ctcagccttg acctttcttt taggctgagg ttgatgtcgc cctgagctca tcgaagggtg
    17581 aactcaacga tgaatgatgg cttatagcaa cgaatgattt gcctcatcgc tctatcataa
    17641 tgccagcagt tgtttaattt tggattcgac gattttacga gaatactggg cttcgacaag
    17701 attggtttgg gctgaaagaa gcgagacctg agcgtttgtc tgttccaaca tagttgccag
    17761 accgagctcg cattggcgcg taacaagttc gtaatctttg cgggctgatt ccagattctt
    17821 ttcctgcaga gcaataactt tattatgatt ttcaaaattg gaatagtaat tcagcaaagt
    17881 ggcaagcaat tcctgttcct tagcctctaa actgacttcg gcgcttctaa ggctgagttt
    17941 gcttttctgg acatttaatt ttttactaaa gccgttgaaa agcccgagat ttaatgacaa
    18001 accccacgaa ccgtagccat cggtcgtagt ggaaccggtc caatctttcg aatagctata
    18061 accagcgcta agcgagggca gatatgactc tcgggcaatt tgcaggctga tttgctcgct
    18121 ttttaacgat tttaatagcg actgatattc gtaattgttc ataaataata attccttagc
    18181 ttcggttaat gatggaatgt caatttgttc aacgttgacc ttggcgatct cgtaatcttg
    18241 gttgggagat attcccatta aaattgcaag tgcgcgtttt tgtttgatta tttcaagctc
    18301 ctgactgatc agttcattct ctatttctgc tttctgaact tcggctttca atacatcaga
    18361 ttcggtcttc gtgccgagtt tatacatcgt ctgaattaat tgcagatttt catcagccgc
    18421 cgtcaggttt tcccgataga cattcaacaa actttgcgca gttaaaatgt tataataggc
    18481 ggtttcgact tcactccgaa tctgggaagc taactcgttg taattaagac gagctatatc
    18541 agtctcattg cgggctaatt ttatatcgga aaataatcca ggcgaataga ttttctggtt
    18601 aatatgcaaa ctagcgctcg atcgcgaact gccatctccg ccgctggcgc tggcactagc
    18661 actcacggat ggaagtagat tcccataggc tgttttggtg ccaattgcag tttgctgaac
    18721 gccgagttca ttctttacta gcgatttgtt attagcaact gccgcttcga tacattgttc
    18781 gagagtaagc tcatcagcgg cgcaaatggt cgtagccatt aacataaaca agccaattat
    18841 tttaattctt ttcataagaa caaatccttt ctattcataa cgcaaggcat cgatcggatt
    18901 aagattagcg gctttgcgcg ctggatacca gccgaagaaa ataccgaccg cgcccgaaaa
    18961 agtaaatgcc agtattacga ttaccggatc aatcacggtt cgcaaatcgg ttaaattgtt
    19021 taaaagggct gaagccgcca aagccagaat aatgccgatg attccacccg ataaactgag
    19081 aacaacggct tccgatagaa actggctgag aatgtcgctg gcacgcgcgc caactgacat
    19141 ccgaatcccg atttcacgcg ttctttccgt gaccgagacc agcataatat tcattattcc
    19201 aatgccgccg actaccagcg atacgcaagc cacggcaccc aggagcaaag tcatagtttt
    19261 agaagtcgaa gaggcaaaat caatgatttc cgattgagtt cgaatattaa aatcatcgcc
    19321 atcagccggt ccaaggcgat gagcttcccg cagaatagtt cggatttcct cttgggcggc
    19381 ggtcattgct tcttcactga tggcgctggc attaatcata tcaatccact gtgaaccttt
    19441 taaccggtat agcacagttg tcgaaggtgc taaaataata tcatcctggt caccacccgg
    19501 tcccgagccg cctttttcct tgagaacgcc gataacggta aaaggaacat tgcgaatgcg
    19561 gatttgctca cccaccggat tatccgcgcc aaatagctcc gtagccaccg ttttccctaa
    19621 cagggcaact tttttgcggg ctaaatcatc gcgttctgta aaaaactctc cactttccag
    19681 ttcccagttg gttatcgaaa aatagtccgg tgaaacgcca ctgatactcg tgtaccaatt
    19741 attgctttta taaatcacct gaccgttagc acggacgacc ggcgaaatag cgctgattaa
    19801 tgtagctgat tccttaattc tttctacatc tttcatcgta aaacggttaa aactcccagc
    19861 gcccatactg gcaccgccgc tacgcgagac tccggggaag atgattatca gattggtgcc
    19921 gagcgacttt atattatcct cgatagcggc ctgcgaaccg cggccgaccc ccaccataat
    19981 tataacggcg ctgacgccga tgattattcc gagcgaggtt agcagactgc gcatccggtt
    20041 tttaaatatg cttttgatag caactttcaa gaggttataa atttttttca ttgctcctcc
    20101 tcttgttgct gtttggcgat tagcatttta aggtcttccg ccgcattacg ccgtttttta
    20161 attagggtat ccttatggag aactccatca cgcatttgaa gaatacgttt ggcataagtg
    20221 gcaaaatcgc gttcgtgagt aaccataaca attgttaaac cttcatcatt taattgctgg
    20281 aagagactaa aaatctccat agacgttttg ctgtccagat tgccggtagg ttcgtcagcc
    20341 agtagaatcg aaggctggtt taccagcgct cgagcaatgg cgacccgctg ttgctgaccg
    20401 ccggaaagct gggtaggaat atggtgcata cggtccccaa ggccaacgcg ttccagcgct
    20461 tctttggcgg cgctttgagg gttctttata cgttgatggc gatcataaat caagggcagt
    20521 tcgacatttt ccaaagcggt ggtgcgtggt agaagattga atccctggaa gacgaatcca
    20581 attttttggt tgcggatatc ggcgcgctca tcagacgtca gcgtactaac gttgacacca
    20641 tccaataggt attccccgcc ggtaggttga tccaaacaac ccagcatatt cattagggtt
    20701 gattttccgg agccggaagc acccattatg gcagtaaatt cacccgccgc cactgcgaaa
    20761 tctaccttgc gcaaggcatg cacttctata tcaccgacct ggtaggtttt actcaattgc
    20821 cggacttcaa ttacgattgg catattattc tctcaattct gtatgtattt tatcggttga
    20881 tttttgtgat taaaatggag gcggaccgcc cataccgcgt tgctgattat tggaagaatt
    20941 gctgaccgaa tttttgccgt tggcaattaa actaacaata attttagttt cttctttaat
    21001 ttcgggattt tccaaaattt cggtattttt agtatcgctc aaaccggttt tcaccaaagc
    21061 cataagaatc tggccggatt cgttgacata ccagattggc gtcggtcgcg ttgatttatc
    21121 taattcttcc ggcgattgat cagccgattg tggtttagga gattgttccc ggaattgtgt
    21181 tttcaccgac tcaggcaatt gttccatttg tgcttgtcgc acctgatgtg ctttcgccag
    21241 catttcttcc gtcggagtaa tttttaacgc cgaatttggc acgatcagca cattttcgcg
    21301 tgaatcaatg ataaaatcca cggtagcggt cattcccggt aataacagtt gttcgtgatt
    21361 atcggcatca acaatgaccg tatagttgac gacattggag acggtttgcg gctgaagacg
    21421 aacctgtctt acgactccat tgaaaatttt atcctgataa ctctggacgg taaagcgcac
    21481 tttttgatcg gttttaattt caccgatatc gctttcgtcc accagggctt caatttccat
    21541 tttggtcata tctcgggcaa tggtgaaaag agtaggcgtc gaaaaactag ccgcgaccgt
    21601 ctgtccctct tcaaccactc ggtcaataac cgttccggaa attggggagc ggatgatggc
    21661 ataaccataa tcggcgaccg ccttatcgag ggcaatctgg gcttgtttta agctgacttc
    21721 gctggtggct ttattggttc gggcatcaat gtattcttgc tccgaaacca atttttgctg
    21781 gaaaagatta gaaatgcgat catactgaaa agcggcttgt tcataaagcg cttgcatttt
    21841 gtgcaaattg gcttctgcct ctttaacgga ttgactcaat agcagtgtat ccaaaaccgc
    21901 cagaacttga tctttcttaa caatgtcatt gtaatccacc agaactttat caatccggcc
    21961 cgaaacctgg gtaccgacgt ccaccgtacc gatcgctacc agcgtaccgg tactggagat
    22021 gatggtttcg atattgccgc gggttacttg cccataggca atctgtatct gtttatccga
    22081 gccgttcttc cgaattagat gccatccgac ggcgaccgcc aaaaccagaa tgacgatcaa
    22141 gcaaattttt ttcttcataa atctttaaat cctttcaatt ttcaaaaccg ctgattagac
    22201 aaaataatga gtgatttcat tccaaatcga acaaactatt ttttatatct gaatggattg
    22261 gaataaattc aacagcctat tggtctaact taatgttttg agaaataatg gagaatttag
    22321 cgtcactaac cgatcaggag ataattgatg cctgtctttc aggaaatgag aacgcatttg
    22381 ccgaaatcat tcgccgttat gaaccgcgag tggcggctac cgttattgga atgctgggaa
    22441 gatgtccgga agccgaggac gttgggcagg agacatttat ccgtttttat aaaagtatgt
    22501 ccaaatttcg aggagaatcg agtttgagca cttatctgac ccggatagcg atgaatttat
    22561 caattaatga actgaaccgc cgcaagcgcc agtcacgttt tgtgcctctg gatgatcctg
    22621 aaatctcgag gaaaacatca ggcgatcccg ggaatcaggc tgattcagaa agcggcgcaa
    22681 ttattcgtcg ggcggttgct caactggcgc cgaaatttcg ttcgaccatc gttttgcgtc
    22741 taatcgaagg ttattcgact gaagaaaccg cccagatttt gcaattgccc gttgggacgg
    22801 tgctgtcgcg tttagcacgc gccaaagtcc aacttatgaa tttattaaca ccctataagg
    22861 agctattatg aaaattgata aaattcgcaa gctattactt ctatccggcg atcgtaactt
    22921 gactgccaag gaacaaaaga ttcttatgtc agccagacaa actaatcccg aactggcgga
    22981 agatgaacaa ctattacaga aaattcaaca aaatcttgct ggcacaaaac aggagtcttt
    23041 tgagccctgg tttgctgaaa ggacgatgaa ccgaattcgc caactcgcca gtcaggaaaa
    23101 gcatccagca agattgtgga atggtttctg gccggcttat agccgggtta ttattggagc
    23161 cgcagtaatt ctggctttga ttgtggtttt taatctcttc actagttccg agccgagcct
    23221 ggcaaaggca ttcggtatta ctcagactcc aatcgaagcc ataatggatc cgatagcata
    23281 tttaatttgg gagtaataaa aatgaagaaa acaaccagaa caactctaat gctaagtagc
    23341 gtcttgattt tgggaattat tataggtttt gtgggcgctt caagttggct acactataat
    23401 cagaaaaaac gttttactca tttccgcaaa ggcgagggct tcgtagtcga aatggaaaaa
    23461 atgattgacc cactcccaga acaaagggat gaaatccacg aaatcttatt acgccattca
    23521 ctctgggtca aacagtttag cgaagagcaa cgcgctattt tacaacgaag tatcgattct
    23581 ttggacgctg agctggctca cgtgcttacc ccagagcaga tgaggcgctt tcaggaaaaa
    23641 gtaaaaaatc agccaaggcg accaggaccg ccacctgggc cgccaccacc acccttcgaa
    23701 gagccacatc cctaaaatcc gggtttgact ttaaagacgt atcgtcttct tgatgaatta
    23761 atatcactta ttaagcttag tgaatataaa atttccgagc tcaatcggta aagataaaca
    23821 attgaaaagc aggaaactga ggcacggatt ccctctgctc acattaatta gagcttcctg
    23881 aaaaggtaaa caaaacatca ggatttttac atttctattt atgaatataa atgaattata
    23941 tgattataaa gcctcacctc ctacagctcc gcgggagtta aaataactat aggcaaacaa
    24001 tatcaccaac cccacagagg ttgatttcat ctaaaaggga cgaacttgtc cattatcggc
    24061 tctatcgtaa aacttgtcta cattttgatt tcttaagtag acctaacaaa aagccggagc
    24121 ataattaact ttcgtttcat tttaaaatgg aataaaaata ggattggctt tgtctaaact
    24181 gcgagtttga gttttttgta acgagaaata aatcacttga aagtaaacga ttttaagcaa
    24241 gtcgttatta ggataaaacg gttgtacggt aaataaattt caaaacaatt ttgtggaaag
    24301 ggaaaaatgc gaaaaatatc aaaaataaca ttattagaaa tgggcgtttt aatggcactt
    24361 ttgtccactt gtaaaaaggc aacggttcag agcgcctgga acgatggcag tataataata
    24421 gatgccgttg ttaaggactg gggctcacgc ttggtgtgga acgaaaaaag ccaggtcaat
    24481 ttgggagtca ccaatgatgc cgaaaattta tacttatgtt tggtaacttc cgacccggac
    24541 atccaccgtc agatattgat caacggtttt attgtcggaa ttgatcagaa gggtagtaaa
    24601 agccatcatt tcggtatcaa atttccattg ggtataggcg accgcggcaa ccaactacta
    24661 atgccgaccg atgaccaacg ccgacagaag ccttttgaac ccagtcaacc ggggcagaaa
    24721 ggtagggaaa atgatttttt ggctaaattt aatgaacagc aaaccgagtt aattttgctg
    24781 ggaccaggtg ataaacaaaa aacgcttgtt cctctggaaa acgacttggg actggaagtc
    24841 aaaatcggat tcgatcgcca gcaacttgtc tatgagctga aattgccatt tagcgtgatt
    24901 agtcagcatc tgcaaattga tcaaatttca aataacaagc ccttgacagt cagtttcaaa
    24961 actgataaag ttgagatacc atccggtcgc ccaggtgata tgatggactc ccccggcgga
    25021 atgggcagac cggaaggtgg tatgggtggt cctggcggta ggatgggtgt tcccgacgct
    25081 agaatgggca atcccggcgg tgatgaacat agcgaaaaga cattttcata tcaagtggct
    25141 gttactttgg cagtgaaacc ataagatttg gtaaaccctt ttgaataata accctcatat
    25201 ctctatcact cactcgccta cactcctcca tggcggttca gcaggacgta cggaagactc
    25261 gtatgagtct tccgttgcct gctgattata taacaccttt tttaactgaa caattattat
    25321 tgtcttatca tcaagaaagg caattaacta tcggatttta ggaaatgact acagtcagga
    25381 tttattcgga ctttcgccat tataagcttt tataatcttt ttaaccaggc ggtggcgtac
    25441 gacatcggtg gtatcgaagt aaacaaattc gattccctca atgcccctca atatttccag
    25501 cgcatcaatt agcccagatt tctggcgatc gggcaaatca atctgggtta tatcgccggt
    25561 gataatcgct ttggaattgg ctcccaagcg ggttaggaac attttcatct gcatcggcgt
    25621 ggtgttttgg gcttcatcca gaattacgta ggcattgttt agcgtgcgcc cccgcatata
    25681 agccagcgga agaacttcaa ttatgtgttg ctcttcgtaa actctgactt tttccggcgg
    25741 caacatatca taaagtgcat cgtatagcgg cgcaagataa ggttcaattt tttcggttag
    25801 gtcgcccggc agaaatccga gactttcacc agcttcgaca gctgggcgcg tcaggattaa
    25861 gcgttgaaca tcgtgattgc gcaaagccgc cacagcaatg gcgactgcca gataagtctt
    25921 gcctgttcca gctgggccaa tgccaaacac taagtcgttt ttcaaggtgg cgtggaaata
    25981 atgttcctga tttaccgagc gcggtttgat cacgccggtt ttcttgaaaa ggataacggt
    26041 atcggcagta atttcgcctg gcaattgctg tccgccaagt tccatattga ttaaagtggt
    26101 aacgtcctgt tcgtcaataa atcctttgcg attgattgtc attagcattt caccgatcac
    26161 gcgctcaatt tggctaagtg tattttcgtc gccttcgaga tttattttcg tgccccgcgc
    26221 gaatattcgc gccccgaagc tctcctcgat tttgttgata aatacgtctc ggacgccgaa
    26281 caccagagct ggatcgactc cttttaattc gatggtttta tttgccatat ttctccatca
    26341 ttgctttcat taattgctaa tttcgcaatt ttattggcaa aatgaaagcc atgcgagaaa
    26401 ccagccagct
//
LOCUS       DAOSMP010000031        25548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_1931_length_25548_cov_16.404503, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000031 DAOSMP010000000
VERSION     DAOSMP010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 25548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25548
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1931_length_25548_cov_16.404503"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..951)
                     /locus_tag="PKX09_06530"
     CDS             complement(211..951)
                     /locus_tag="PKX09_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU17512.1"
                     /translation="MLGKAKSRYMSGKKKALLLVSVIILPVLVFAQYTGGSYDGYAMN
                     EDVEDNSLPVELSLFTATPGNGIITLKWLTESEIENLGFNIYRSTKSNDEFLMLNAEL
                     IAGAGSSSEKHEYSYVDQDVKNGITYWYKLEDIDYNGKTEFHGPISATVLGPKEFCLH
                     SNYPNPFNPVTTISYDLAEDVYVELTVYNMIGEKVTTLVKGNQPAGYYNVEWDGRNSQ
                     GRIVPSGMYFLRISAGSYNKTNKMAFVK"
     gene            complement(1031..1393)
                     /locus_tag="PKX09_06535"
     CDS             complement(1031..1393)
                     /locus_tag="PKX09_06535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOU17513.1"
                     /translation="MGRFKNEELLDRVFTWVNAVLDLVEIIPNTTIGKNIQYQLVKSS
                     TSVGANLEEADAAYSRKEFTSCVNIAKRESKESRYWMKILRFRKMVDFDKLNPIINET
                     EEIIRILFAIVKGTQNND"
     gene            complement(1502..3034)
                     /locus_tag="PKX09_06540"
     CDS             complement(1502..3034)
                     /locus_tag="PKX09_06540"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU17514.1"
                     /translation="MKKSTIITVSAVLIALAMTMPLWANHVVLENVSLTEQNTTDDYT
                     HIEFDISWENSWRLDGVAAPNNWDAAWVFAKYNIVGDDPDVGWKHCTLNTSGHTAPTG
                     SVVENPTSDNSQTGVFIYRSADNKTTEGNAASVDWDNAKLRWNYGTDGIEDGAIVDVK
                     VFAIEMVYIPQGEFHLFNGESGNFYAEFNSGNTISSEDAIGENTITWIMKDTYCGAGT
                     SDGIAGYNAALGVNYPKGFKAIYCMKYEISQGQYADFLSLLTDAQDDNRYPNANGSYR
                     HTISGSYGSYSASRPDRACNWISWADGLAYADWAGLRPMTELEYEKICRGPETAGIEY
                     AWGNTTITAATTISGTENGTEIITNPGANCCYSGQTFTGGDKGKGPLRCGIFATGSSS
                     RQQSGASYYGVMELSGNLWERYITVAKYCSNGSTWIETGVSSFDGRHGDGVLTTNGFA
                     DVTNWPSPTVTSGNTAFGSSFRGGDWSGGAAGLCVSDRYSAAAPYAYRRQYDGFRLSR
                     TQ"
     gene            complement(3458..6046)
                     /locus_tag="PKX09_06545"
     CDS             complement(3458..6046)
                     /locus_tag="PKX09_06545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HOU17515.1"
                     /translation="MNKKVIILNFFMMVYIMMALPHHLLAQGAGKALNFNGTYQYVSV
                     PNSPSLNVTNALTLEAWMKPTGENWDYQVPVTLNPATPVADYQVKVELTTSNFNYAHT
                     KSDGSDIRFYDGSGTELSYWIENWNTSGTSIIWVKVPTAGTTNIFMYYGNYSASSESN
                     GDNTFEFFDDFEETSLKTGWTFWNPGGDDGYSLTERPGWLRIKVNEDSDSWSAVAKAP
                     FVYWTHPNPNTNFVTQTKEDATGIDNRYRHSLLAYIRSFDAGSYNKGYFGSCESATTV
                     KFEADGYRGSQCTTGQAIHCTRFRKDASKLYYDWSNDGITWNNCGSYTLPSIPSYWGL
                     GGKSWDGGGSFNADFDYFFVRKYASSEPVATVGNETAPGISKAGAYGIGANTTTAFVS
                     INNQTISGEISSGWNHIAQTYDGSQQKLFINGNLTTSSDLTGNINSNSNNLMIGSLFN
                     GTIDEIRIWNVARTEEEIRANMCKKLTGSESGLIGYWRLDEGSGTTANDATSNNNHGM
                     LTNSPSWVWSGAAIGDESAFDYEGTNPADFSVNLTHSDGDNLTVTGDGGTISGIQVYR
                     VDTNSMREGSTKPGESWVMDPLRNWGVFITGTNPSYTFTYNYNGHPGIAVEGDLRLAI
                     RDNLADDSWEDADAELNQSDNTLTLTNQTGTEYALGSINSDNSLPVTLTSFTATPGNG
                     IITLKWLTESEIENLGFNIYRSTNENGEFAMLNAELIAGAGSSSEKHEYSYVDQDVKN
                     GITYWYKLEDIDYNGKTEFHGPISATVLGPKEFCLHSNYPNPFNPVTTISYDLAEDVY
                     VELTVYNMIGEKVTTLVKGNQPAGYYNVEWDGRNSQGRIVPSGMYFLKISAGSYNKTN
                     KMVFIR"
     gene            6422..6883
                     /locus_tag="PKX09_06550"
     CDS             6422..6883
                     /locus_tag="PKX09_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17516.1"
                     /translation="MGTQQILLIVLSSFVVGIAVIIGITMFHNQAINTNRQAVISDLN
                     NFAAQAVAYYKLTESMGGGGNGSPGFVEGDNPSTILLSYLGFDQEGSGEIAYKHTNDA
                     GTYSITSQTAHTLTINGVGKEKVGASDNVEVNLSINMQEDPSQQITIDVAH"
     gene            complement(7098..>8585)
                     /locus_tag="PKX09_06555"
     CDS             complement(7098..>8585)
                     /locus_tag="PKX09_06555"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOU17517.1"
                     /translation="QAPLPFFTLERTLPQPKHKVNLLRDVTAKAITDLVCRENDRQVL
                     ITLNSGIAYLLFHLYGINGNVYLLDSSGVIRDSFKKAKKLVNFELSNFRPANRLFPDY
                     ADFTSSLRQHSDQSLMTFFTKSFQPILPKILVTEICYRTNLAPQYSIINLTDEQVERL
                     ATTIDELADAIVTSLPRIYSGEPPIFSLIELQSLSNREFAEYDSVIAAQNQFIQDYLN
                     LYRLKEKKKTLSRQTEFLLSTTQRKLIKQQSELAALPTADNYREWADTLMATASTIPH
                     HSNSVTLPQLTDEARQITIPLEPKLNPIQNAERYYAKSRQIEKARAELIQKIAEAEQL
                     IQRLENTQKQIAEADDFKTLRTLSTHLPAQPQSSIENIHQPFININLDGWEILIGKSA
                     RDNDELLQKFARPNDFWLHAQGTSGSHIIVRNPGKVASLPRPILEKAAGLAAFHSKAK
                     HSAVVPVIYTQCKYVTKPRHSAPGAVTVKFEKTIMAEPLDPKKFL"
     assembly_gap    8586..8685
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8686..9034)
                     /locus_tag="PKX09_06560"
     CDS             complement(<8686..9034)
                     /locus_tag="PKX09_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17518.1"
                     /translation="MQPIFIYEETKWNLSAPHELYSCNRLPTKQHPLGALINALLVLL
                     FNMFNSWLHIERLAREYQQLIGGAICGMPFTYQKDHLHIPLEMAGEWECLHFSVQAPL
                     PFFTLERTLPQPKH"
     gene            9072..10001
                     /locus_tag="PKX09_06565"
     CDS             9072..10001
                     /locus_tag="PKX09_06565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOU17519.1"
                     /translation="MTVWTGLTNFTIDLIGDLQLESMTGYSRTEYRSGGQVISVEIYS
                     ENNRFLDLTFNLPEPLEPYTEELKRLVAGDLRRGRVRITIAITGSEKTPEVQLNTAFL
                     TKYYRALCDLARELQIEEPVTLQNLLTYPEIFMTDSADTDVAELFTQVKDLLRQAVAE
                     LIAMRRREGEFITRELQSHLQFILEKCSEIEKLSQQSRQEYFTKYQQSLAELCQNCQL
                     DENRLIQEAGILAKKLDITEECTRLRSHIQQFTLLLQSEEPVGKQMNFLAQEMNREIT
                     TIGSKSESAAISHLVVNLKNELEKIREQIQNVI"
     gene            9998..10567
                     /gene="gmk"
                     /locus_tag="PKX09_06570"
     CDS             9998..10567
                     /gene="gmk"
                     /locus_tag="PKX09_06570"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOU17520.1"
                     /translation="MKPKGLLVAIAAPSGTGKTTVCHELMKRDDRFVFSVSCTTRPPR
                     PNERDGVDYHFISREQFLKNIHKGKMAEWQEIFGNYYGTLKDSIEQALRDGKILLLDL
                     DVKGTLNLKKNYPDQLISIFLLPPSDTELDRRLQKRGTDDSSSIAIRKARIPDELELS
                     EQFDFQIVNDNLEATVNKIINIIEEHLKK"
     gene            10564..10884
                     /gene="rpoZ"
                     /locus_tag="PKX09_06575"
     CDS             10564..10884
                     /gene="rpoZ"
                     /locus_tag="PKX09_06575"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOU17521.1"
                     /translation="MTETLPFSKLTRKSDDIFELVVVAAKRARQINALRMAQYPLPTM
                     VEDQEETFEETPPEITDEPAWDYLEKPTTLAINEILEGKINYRYMTPEEEKPLEEFDS
                     EFSE"
     gene            10868..12085
                     /gene="coaBC"
                     /locus_tag="PKX09_06580"
     CDS             10868..12085
                     /gene="coaBC"
                     /locus_tag="PKX09_06580"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOU17522.1"
                     /translation="MNLANRKIVLGLTGGISIYKSAGLLRRLVNDYKADVTVIMTDAA
                     QKFMSPLIFETFSHKPVLTDMFTGQKIETRHIDLATEADLILVSPATANIVAKAANGI
                     ADDLLSTIILVGGNKTVFALAMNDNMYANPITQENISRLRRLGYDIIEPEEGALACGS
                     TGRGRLADETTILAHIDRHLNGNKWLKDKKVIVTAAPTREYIDPIRFISNRSSGKMGY
                     ALAEIAFRESADVLLISGPSDLKNTVGVKTIRVETANEMRSAVLSEAAEADYLFMAAA
                     VEDLSPVQIATQKIKKESGISNLKLKYSPDIIMDFRKHNRQACVVGFSVEIESAEHPG
                     AKARSLAKLNDKEMDFIVWNDPNVTGAAFGHETNSVTLLSKDGQEWVFPLASKRDIAD
                     SIISTVIKNYKKA"
     gene            12086..12763
                     /locus_tag="PKX09_06585"
     CDS             12086..12763
                     /locus_tag="PKX09_06585"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOU17523.1"
                     /translation="MTRLYDDIKTFFKQQAELFPPEIYLISENGEESTPKTDAATQLA
                     ELERICQNCRQCHLSETRTKVVFGTGNPHAKLLFIGEAPGVNEDLQGLPFVGRAGNLL
                     DKMLKAIGLTRAQVYIANVLKCRPPENRTPNVGEVEKCEPYLLKQIAIIQPELIICLG
                     LTSAKTLLRVEYTLEKMRGQIFQYHNVDLIVTYHPAALLRNENLKKYAWEDFKKIKNL
                     YLDKTGE"
     gene            12764..14143
                     /gene="dnaB"
                     /locus_tag="PKX09_06590"
     CDS             12764..14143
                     /gene="dnaB"
                     /locus_tag="PKX09_06590"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOU17524.1"
                     /translation="MALNDGEIIRVPPHSDEAEVSVLGAMMLDKEAVSKALQYLDYTA
                     FYKEAHQHIFQAMTDLFNEGQPVDQVSVIDQLKRKKMLEQVGGAYYVTGLVEATPSAA
                     NVEYYANIVLDMAILRRLIVASNEIQTESYEARDSVQDILDTAEQKIFAISENRMKGG
                     FKNFVDVLNRTFEHIDSIHSRKGHTSGVPTGLLDLDELTSGFHPGELIILAGRPGMGK
                     TALALTIARNAAVEYNMPVAFFSLEMADYQLALRILCAEARLDSHLVRKGKLPKEQWQ
                     RLSMQTGRLANAKIYIDDSPVLTMMDIRAKARRAKAEHDIQLVIIDYLQLIKGHRAEN
                     RQQEITEISRGLKALSKEIGVPVIALSQLSRAVEGRADHRPQLSDLRESGSLEQDADV
                     VIFIYRKSFYDKREGSAASDEDERLAEIIVAKQRNGPTDTVEVIFIDKYTRFENKGAQ
                     REEIITTPF"
     gene            14143..16323
                     /locus_tag="PKX09_06595"
     CDS             14143..16323
                     /locus_tag="PKX09_06595"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOU17525.1"
                     /translation="MATEILNKVLPRAKQRPQKFLTLISKLNQRAPLTKDEENFLWEV
                     YSFARNSHRGQRRQSGDSFFEHPYHTALILADLHMDPITVAGGLLHDVIEDTEVTVKD
                     IAEKFGDDVAKLVEGVTKISGITFRNRQEEQADNFRKMLLSVAEDIRVLIIKLADRLH
                     NMQTLDSLPPIKQRRVAIETRDVYAPLAHRLGMFKLKSEMEDLVLKALDPDAYRFLEK
                     KVNEKRSEREKYIKVFTQPIIKALNEQGIKAKIVGRPKNFYSIYKKMKTQNKPFEEIF
                     DLLAVRIIVDTKENCYTVLGIVHDLFTPVMDRFKDYIANHKANFYQSIHTTVYGPSGR
                     MVEIQIRTTEMDEIAEEGIAAHWRYKEGRDKPDDIDKYVKWLRDLIEILQTESTGAKD
                     FMDTLKIDLFKDEIFVFTPMGDLIRLPKGATPVDFAYEVHTEIGTRCIGAKVDGKMVP
                     LNSELRSGQTIEIITSDSHRPSYAWLKFVTTAKAIGAIKRYLRKAQFEESINLGKEIL
                     EKENQRNKELRFLKTVQESFEELGFSDLNKLYAAVGSGVLTIPQIYAKLFPQKQEQLV
                     RQDLDELFIETARKSVKGIKVQGISNLMVSFAKCCNPIPGDQIIGFVSRGRGVIIHRS
                     DCSNIPSLLEEDERLLDVEWDIAGTQAFLVRLKIMAQERKKFLKDVTDIMASTDTNII
                     SVSGDVDESIIHLNLVIQVENLRHLNKILAKLQNVQGIISVERK"
     gene            16342..16647
                     /locus_tag="PKX09_06600"
     CDS             16342..16647
                     /locus_tag="PKX09_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOU17526.1"
                     /translation="MKVKTYTKKDVVRRTAEALDTKLVDTAEIVDGVFMVLREMLSES
                     DPRIRIEIRNFGVFEVKPTKAKPKARNPRTNEEVYVPARRKTHFKPGRLLKERMKKA"
     gene            16696..18249
                     /gene="lnt"
                     /locus_tag="PKX09_06605"
     CDS             16696..18249
                     /gene="lnt"
                     /locus_tag="PKX09_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOU17527.1"
                     /translation="MMKVKINLWWLILISGVLSGLAFPPLPLGFLAFFALVPLIKAFC
                     DDEFHNGFEKGLVYGLVLNLAIMYWLALNKGTTWYWATLSMVAAVIFLAMNYAIIGLS
                     VGFIGRRLGRNVGIWSFPIIWTAIEYLRSFGSLGFTWNNLCYTQSHAIQLIQSAAVVG
                     PYGISGWIVVINVLIFRLLENLEDYRQVSRRMVIIIALFLIPAIYGMVVLQKPSDEND
                     RRLVNVGLIQPNIDPNAKWDQESFKKNMQLLHDLTDSVATQPLDLVVWPETATPTFLR
                     RNHYAVLTEIREHIEQLQINLLTGTPDLERAGEDYRYYNATFLLRPHSNRIEDYRKIH
                     LVPFGEYIPLSGYFPELSNLNLGQGNFQAGKEVKLFQIPLRVDQRPVSDTVLTFNSVI
                     CYESTFPDLVRQATRQGSELLVIVSNDAWFGNTSAPFLHAEIGRWRAIENRIPVVRSA
                     NTGISLILDQYGRNLQSLPFGKSGWLSAPVTQGNPQTIFVRYGNWPARLCVILALLLI
                     GYSIIRRKK"
     gene            18246..18932
                     /locus_tag="PKX09_06610"
     CDS             18246..18932
                     /locus_tag="PKX09_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17528.1"
                     /translation="MKHLFCPSLLGLLIIFILAGSGTAQIDSTLVAQPDSLPQLTQSQ
                     ARLLLLKSAVLPGWGERSLNYVERSHYFNASEIALWVTYFAFYWYGNAVAADMEAYAA
                     THAGVNPDGKDQYYFTDIGNYENIYAYNEQKLRYRAVNLLYPVTPEYYWAWDSESSRR
                     HFDKLRVKSATALRNATFAIGGLVANRLFSMLDVIVLTRNRLEMPDIDIESNWQPYQD
                     GMSFMLSLKF"
     gene            18934..19680
                     /locus_tag="PKX09_06615"
     CDS             18934..19680
                     /locus_tag="PKX09_06615"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOU17529.1"
                     /translation="MKNLDPRVKILWLMIAVIGIFRLKVWYAQLGVALVLGTIIIFRT
                     ISTKESFKVLRILLVFLPLTFLIHILFGTNLLATIFRSAPRDSWSVLLFTPLTFTLRI
                     GNLIALMALATHWLKALEVLDALYHLLKPLRKIGLPVDDIFQIIFIAVRFIPLIQEEY
                     QRLDDSWRIFMNRDSGGLFGQIERLRTILVPLMVVSFRRAEVLAEAMTVRGYNSQVKR
                     SYIGRLHWQTPDWMGLVAGCGSLVLMVINI"
     gene            19680..20426
                     /gene="truA"
                     /locus_tag="PKX09_06620"
     CDS             19680..20426
                     /gene="truA"
                     /locus_tag="PKX09_06620"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOU17530.1"
                     /translation="MGRRFKIIMQYDGSNYCGWQRQRYQPSVQAKIENALQPLNQNQP
                     VMVIAAGRTDSGVHARRQTAHFDLVTDLSPETIRKALNATLPDDIYIDYCQEVGKEFH
                     ARFTAINRTYHYQVVVNPNVFLRKYVWLVNFRFDLELLQECAELVKGEHDFTALCRAA
                     TETENKMATIYESRWEEQDELLVYIITANRFLHAMVRMLVGSMMEVARGKYSIGAFKN
                     LLGKNDTKMQVYTAPAQGLFLWNVKYQEDN"
     gene            20429..21073
                     /gene="fsa"
                     /locus_tag="PKX09_06625"
     CDS             20429..21073
                     /gene="fsa"
                     /locus_tag="PKX09_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOU17531.1"
                     /translation="MKLFIDTANLEEIRKAQSWGLLDGVTTNPTLLSKEKGNFKEILL
                     KICETVNGPVSGEVVSLDAEGMVREAEDLAALHKNMVIKVPITLEGLKAIKILSEKGI
                     RTNTTLIFSPLQAILAAKAGATFISPFVGRLDDIHQVGMELVQQIKTIYDNYGIRTEI
                     IVASIRNPLHIVEAALIGADICTIPFNVMEKMVKHPLTDKGITQFLEDWKKVEK"
     gene            21070..22170
                     /locus_tag="PKX09_06630"
     CDS             21070..22170
                     /locus_tag="PKX09_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOU17532.1"
                     /translation="MKKINSVLISSLFIGVSLLLAVPQEQQEISNSRRNAITAAIEKV
                     SPAVAGINVTAIEEYVASPFFNDPLWSLLFPEQIYRRRVKSIGSGVVISSDGYIVTNT
                     HVVEGAEEIVVTLMGGKEYKAKLVGIDNISDIALLKIDGKSFTYAKLGSSEDVIIGEW
                     VIALGNPFGLFSVNYKPTATIGIVSGTDLDFGPQQSGRLYQDMIQTDAAINTGNSGGP
                     LVNALGEVIGINTFIFTGGQFSEGSIGIGFAIPVDRVKAVVANLKQYGKDSRTFKIGW
                     ELQNVDSFLASYLNLPPNTGVIVTRVEKSTPAERSGLKVGDVIISINGKKVARAEEIY
                     AYIQENLLRQGDKIKVRFLRGNREYETQLVLE"
     gene            22174..23484
                     /gene="purB"
                     /locus_tag="PKX09_06635"
     CDS             22174..23484
                     /gene="purB"
                     /locus_tag="PKX09_06635"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOU17533.1"
                     /translation="MIERYTRPAMGRIWSEENKLATWWRVELEVCRVQNERGIIPDEA
                     LQEIESRAKFSLKRIQEIEEEVKHDVIAFLTNISENVGPAARFIHKGMTSSDLLDTAL
                     ALQLSEAGQLILADLNRLEKILTEKSIEYKDCLMIGRTHGVHAEPITFGFKLLIFREE
                     IRRHIKRFELALDDIRVGKISGAVGTYQHIEPEVEERVCHNLGLEPAPVTNQIIQRDR
                     HAFFIATLALIGTTLEKIATEIRHLQRTEVLEVEEFFSEGQKGSSAMPHKRNPVLSER
                     ICGMARLLRGYAVTAMENIPLWHERDISHSSNERVILPDACICTDFILNEMSKVLTNL
                     LVYPENMRRNLEGMHGLIFSQEIMLAIIEKGASREEAYTIVQNNAMKVWQSGSDFKEL
                     LLKDENVLKWLNPTEIESYFNYEKILTRINRIFERIDSGNYEKE"
     gene            23471..24184
                     /locus_tag="PKX09_06640"
     CDS             23471..24184
                     /locus_tag="PKX09_06640"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOU17534.1"
                     /translation="MKKNEKLYEGKAKIVYSTDDPELVIQFFKDDATAFDGIKKGQIG
                     GKGVANNLISCHLFSLLENNGIATHFIKKLSDNEMLVRRVKIIPVELVVRNVAAGSLC
                     KRYGVPEGQVFSEPIVECYYKNDAFHDPAMNDDHIIRFNLANAGELSAIKATALRINQ
                     ILKEYFDSIGIILVDFKLEFGRYNDQILLADEISPDTCRFWEKGTNRKLDKDRFRFDL
                     GEVEEAYTEMLKRITGIQK"
     gene            24181..24816
                     /locus_tag="PKX09_06645"
     CDS             24181..24816
                     /locus_tag="PKX09_06645"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOU17535.1"
                     /translation="MIAKESLRLIIPMLIVAVLAIFLAKYFDLGIMMVLFWVTLVSII
                     FTLYFFRDPERITPQNPAFLIAPADGKVVGISEVNDPFVGGGIKIAIFMSPFDVHINR
                     APYSGIIRQIEHKRGKFHSAFKPEAAIENEQCRITMETDNLKLAVTQIAGFCARRIIS
                     RVKVSQNLEKGERFGLIMFGSRVELIIPSKLKINICPGAKVKAGETVIGEI"
     gene            24831..25541
                     /gene="pssA"
                     /locus_tag="PKX09_06650"
     CDS             24831..25541
                     /gene="pssA"
                     /locus_tag="PKX09_06650"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOU17536.1"
                     /translation="MLPSILTILNLFAGFLALLQISQGHYIAAVELMIVALIFDGLDG
                     KIARFTHQTSDFGIEFDSLCDMVSFCLVPAVLIHELFVSELGFIGGLISFFPLLFGGV
                     RLARFNLQAGVGKKSYFIGMPTPTNALAIGSYIWFSYKVLGNYGNPKIILPLVVVLSF
                     MMVSNIRFSVNLKLGFRRGLPTAFKTGMIILITIGMVIFNGYVIFPLVMVYIITHLLQ
                     WLIGYDEPRIHLARRKAH"
BASE COUNT         7126 a         5287 c         5605 g         7430 t
ORIGIN      
        1 ttttttcaga aaacttacaa aaattagaca tgaaaacaac caaaggcaac ggtggaaaaa
       61 atttttaact acaaggcacg aaaaataaag ctaagattga aaaattagga cactatgttt
      121 cagattactg attaaaaata gtccccgaat tctccagcgg tgtctcttcc ggaacgtccg
      181 ggctggtctt ggcctcagcc acgaccgagc ctattttaca aacgccatct tattcgtttt
      241 gttataacta ccggcactaa tcctaagaaa atacatcccg gacggaacta tcctgccctg
      301 actatttcta ccatcccatt ctacattata ataaccagct ggttgattac ctttgaccag
      361 tgtggtgacc ttctcgccta tcatattata gactgtcaac tcaacgtaaa catcttcggc
      421 taaatcataa gaaatcgtcg tcaccggatt aaacgggttg ggataattcg agtgtaagca
      481 aaactccttc ggaccaagta ctgtggcaga tatcggtccg tgaaattcag tttttccatt
      541 gtaatcaata tcttccagtt tataccagta ggtaatgcca ttctttacat cttgatcaac
      601 ataactatac tcatgctttt ccgaactgct tccagcaccc gcaattaatt cagcattcaa
      661 cattaaaaat tcatcattgg actttgtgct gcggtagata ttgaagccca ggttttcgat
      721 ttcactttcg gtaagccact tgagggtgat aattccattc ccaggcgtag cagtgaataa
      781 gctgagttcg actggcagtg aattgtcctc gacatcttca ttcatcgcat acccatcata
      841 actgccgccg gtatattggg caaataccaa aaccggcagt atgataacgc taactaatag
      901 taatgctttc tttttccccg acatgtatcg ggatttcgct ttgcccaaca ttttgactcc
      961 ttgttttttt ataaatattt ttttacaaat ataaaaactg aaagctgaaa tccgaaagct
     1021 gaaaatatca ttaatcatta ttctgagttc ctttgacaat tgcaaaaaga atcctgatga
     1081 tttcttctgt ttcgtttata attggattta gtttatcaaa atcaaccatt tttctaaatc
     1141 ttagtatttt catccaatag cgagactcct ttgactcacg tttggcaata ttaacacaac
     1201 ttgtgaactc ttttctgcta taagccgcat ctgcttcttc taaatttgcg cctacagatg
     1261 ttgaagattt aactaattgg tattgaatat tcttaccaat cgttgtattc ggaattattt
     1321 caaccaggtc taaaacagca tttacccagg taaatactct atccaataat tcctcattct
     1381 taaatcttcc catctaaact tcctttccta atttcagctt tcctattttt cagctttcat
     1441 ctttcagctt tcctaatttc agctttccta atttcagctt tcctaatttc agcttttttc
     1501 tttactgagt cctcgaaagc cgaaacccgt catactggcg tcggtaagcg tacggggcag
     1561 ccgcgctgta gcggtccgac acacacaagc ccgccgcacc accgctccaa tcaccgccgc
     1621 gaaaagaact cccgaaagca gtatttccgc tcgttaccgt tggcgagggc cagtttgtaa
     1681 catctgcaaa accgtttgta gtaagtacgc cgtctccgtg ccgtccatca aaggaactta
     1741 cacctgtctc aatccaggtg ctaccattgg aacaatactt tgccacagta atataacgct
     1801 cccagaggtt gccgctcagt tccattacac cgtaatagga agcgccggat tgttgacgac
     1861 tagaactacc cgtagcaaaa atcccgcaac gcaaaggtcc ttttcctttg tctccaccag
     1921 taaatgtctg accactgtaa cagcaatttg cgccgggatt ggttatgatc tctgtgccat
     1981 tctcagttcc gcttatggtg gtcgcagcag taatagtagt attaccccag gcatattcaa
     2041 ttcctgcggt ttctgggccg cggcatatct tttcatattc taattcagtc atcggtctta
     2101 gtcctgccca gtctgcataa gccagtccgt ctgcccaaga gatccaattg caggcacggt
     2161 ctgggcggga tgcagaataa cttccataac tgccggatat ggtgtgtcgg taacttccat
     2221 ttgcatttgg gtaacggtta tcatcctgag catctgtaag taaggagagg aagtcagcat
     2281 actgcccctg tgagatttcg tatttcatac agtagattgc tttgaaacct ttgggatagt
     2341 ttacaccgag tgctgcatta taaccagcaa ttccatcact agttccggca ccgcaatagg
     2401 tatctttcat aatccaggtt attgtatttt cgcctatggc atcttcgctg ctgatggtat
     2461 taccgctgtt aaattcggcg taaaaattac cgctttcacc gttaaacagg tgaaattcgc
     2521 cttgcgggat atacaccatc tcaatagcaa agaccttaac atcaacgata gcaccatctt
     2581 caatgccgtc agtaccgtaa ttccagcgga gtttggcatt gtcccagtcc acactggcag
     2641 catttccctc agttgtctta ttgtccgcag aacgataaat gaatacccca gtttgtgagt
     2701 tatcagaagt tgggttttct actactgaac cggttggtgc tgtatgtcca gatgtattca
     2761 aggtgcaatg cttccatcca acatcagggt catcacctac aatattatat ttggcgaata
     2821 cccatgctgc atcccaatta ttaggcgcag ccacaccatc cagtcgccag gagttctccc
     2881 acgagatgtc gaattcgatg tgggtgtagt catcggtggt attctgttca gtgagagata
     2941 cattttctaa taccacatgg tttgcccata ggggcattgt catcgccagt gctatgagca
     3001 cggcggaaac tgtaataata gtagactttt tcatcttttc ctccatgttt tgttattgaa
     3061 tatttttgga gtgcaccgac cgtgtcggtg catgcatcaa cacggttgat gcactccata
     3121 atacattcct ttgccctctg ctctatgctc ttcgctcttt gctattctcc aatcttcaat
     3181 cttaaatctt ttgccacgaa tgcacacctt aaaaaatccc ctcattttaa ttaaatttac
     3241 cccccctatt gaaaaagtca agcttttttt taaatatcta aagtcagcca caaagacacg
     3301 aagacaccaa gattattaaa tatttcatta ttgaagattg aatatttttt ctgcccacga
     3361 aagacacgaa cacttatttt caattttttc aatcttcaat cttcaattct ccaatcttca
     3421 attgtgcctt ggtgccttag tggctgaact gttactttta cctgataaac accatcttgt
     3481 tcgttttatt ataactaccg gcactaatct ttagaaaata catcccggaa ggaactatcc
     3541 tgccctgact atttctgcca tcccattcta cattataata accagctggt tgattacctt
     3601 tgaccagtgt ggtgaccttc tcgcctatca tattatagac tgtcaactca acgtaaacat
     3661 cttcggctaa atcataagaa atcgtcgtca ccggattaaa cgggttggga taattcgagt
     3721 gtaagcaaaa ctccttcgga ccaagtactg tggcagatat cggtccgtga aattcagttt
     3781 ttccattgta atcaatatct tccagtttat accagtaggt aatgccattc tttacatctt
     3841 gatcaacata actatactca tgcttttctg aactgcttcc cgctcccgca attaattcag
     3901 cattcaacat tgcaaattca ccattctcat tagtactacg gtagatattg aagcccaggt
     3961 tttcgatttc actctcagta agccacttga gggtgataat tccattccca ggcgtagcag
     4021 taaaggaagt aagagtaacc ggtaaggaat tgtcgctatt tatagatcca agggcgtact
     4081 ccgttccagt ctgattggtt aaagtaagcg tattgtcact ttgatttaat tctgcatccg
     4141 catcttccca ggaatcatca gcaaggttat ctcttattgc cagtctaaga tctccttcaa
     4201 cggctattcc aggatgacca ttataattgt aggtaaaagt ataacttgga tttgtgccgg
     4261 taatgaatac tccccagttt cgcagaggat ccattaccca actttcgccc ggtttagtgg
     4321 aaccttcccg catggaattg gtatctacgc gataaacctg tattccgcta atcgtgccgc
     4381 catcgccggt tactgtgaga ttgtctccat cgctatgagt gagatttact gaaaaatctg
     4441 ccggattggt tccttcataa tcaaaagcac tttcatcccc aatggctgca cctgaccata
     4501 cccacgatgg tgaattggta agcatcccat ggttgttatt cgatgttgca tcattggcgg
     4561 ttgttccgct tccttcgtcc aaacgccaat aaccaattaa tccgctttca ctgccagtaa
     4621 gtttcttaca catatttgcc cgaatttctt cttccgtgcg ggctacattc caaatcctaa
     4681 tctcatcaat tgtgccattg aataagctcc caatcattaa gttgttggaa tttgaattga
     4741 tgtttcctgt taagtctgag cttgtggtta aattaccgtt tatgaataat ttctgttgtg
     4801 agccatcata cgtttgagca atgtgattcc atcctgaaga aatttcccct gaaatggtct
     4861 gattgtttat ggagacaaag gcagttgttg tatttgcacc aattccataa gctcctgcct
     4921 ttgaaattcc tggtgcggtt tcatttccaa cggttgcgac aggctcagac gaagcgtatt
     4981 tacggacgaa gaaataatcg aaatcggcat taaagcttcc ccctccgtcc catgattttc
     5041 cgcccaatcc ccaataggat ggaattgagg ggagcgtgta cgagccacag ttattccaag
     5101 ttattccgtc attactccag tcgtaatata gtttagatgc atcctttctg aatcttgtac
     5161 aatgaatcgc ctgccccgta gtacattgcg aacctcgata gccgtcagct tcaaatttta
     5221 ctgtagttgc cgactcacat gagccaaaat agcctttgtt ataggatcct gcgtcaaaac
     5281 ttcttatata agcaagcaag gagtggcgat acctattatc tataccggtt gcatcttctt
     5341 ttgtttgagt aacaaaattg gtatttgggt ttggatgagt ccaatataca aaaggagctt
     5401 tcgccaccgc tgaccaggaa tccgaatctt catttacttt tattctcaac cagcccggtc
     5461 tttcagttag tgaatagcca tcgtctcctc cagggttcca aaaagtccat cctgtcttca
     5521 aacttgtctc ttcaaaatca tcaaaaaact caaaggtatt atctccattg ctctccgaac
     5581 tcgcagaata atttccgtag tacataaata tgttagttgt tcctgcagtg ggcactttta
     5641 cccagattat tgaagttccg gaagtattcc agttttctat ccagtaactt aattctgtac
     5701 cacttccgtc ataaaaacgg atatcgctcc catcactttt ggtgtgagca taattaaagt
     5761 tggaagtggt taattcgact ttcacttgat aatcagctac cggagtagca ggatttaaag
     5821 taacaggaac ctgatagtcc caattttctc ctgtaggctt catccaggct tccaatgtca
     5881 aggcattggt tacatttaaa ctaggtgagt tagggacaga aacatactga tacgtcccat
     5941 taaaattcaa tgctttaccc gctccctgag ccaataggtg atgcggcaaa gccatcatta
     6001 tatataccat cataaagaaa tttagaatga tcactttctt attcattttt ttcctctcat
     6061 ttcttatttt aagtatactc taacttattt ttatccatta aatagtaatt aaacttaata
     6121 ctctaatctt cataagtaat attaacaatt ttcaattgaa aatatcaagg atttttagaa
     6181 tgctgttgag gctgaaaatg agagatgatg agaaagggag aaaccggctt gttaaatttt
     6241 gtgttgaatt tttgggagcc ttgataaatt gaatcgtcgt aaaattgtta agttaagaaa
     6301 aagaaaagtg aatttgcact taccttataa attgtatttg aaattagaat taattgaata
     6361 aattatattc agttttaaaa ttgattgacg gatggttttt aaccaaaaat attaattagt
     6421 aatgggaaca caacaaatcc tgctgatagt cttgagctca tttgttgttg gtatcgctgt
     6481 tattatcggt ataacaatgt ttcataatca agcaataaat actaaccgac aggcggtaat
     6541 atctgactta aataattttg cggctcaagc cgttgcctat tataaattga cggaaagtat
     6601 gggcggtggt ggaaatggtt caccgggatt tgtagaagga gataatccat caaccattct
     6661 tctttcatat ttaggttttg atcaggaggg ttccggtgaa attgcttaca aacacaccaa
     6721 tgatgctggt acctatagta taacaagcca gacggctcat acattgacca ttaatggggt
     6781 tggaaaagaa aaggttggtg caagtgataa tgttgaagtg aacttgtcaa ttaatatgca
     6841 ggaagacccg agtcagcaaa ttaccataga tgtagctcac taattttatt tcgatgtctt
     6901 actttttggg tattggagat tagtgttcaa acctccgaac ttaatgtgcg ttttatcgct
     6961 actttattta caactttgtc tttcatttca accttaactt ttacctcatt tcttttaacc
     7021 ggcgaatcaa tatttttatc ggtaaaatcc aatgtcccta aactttaaaa tgtcttggtg
     7081 tctttgtgcc ttcgtggtta gagaaatttc ttaggatcga gcggttccgc cataattgtc
     7141 ttctcgaatt tgacggttac cgcgccgggt gccgagtgcc gtggtttggt aacgtactta
     7201 cattgcgtat agattaccgg cacaaccgct gaatgtttgg ctttgctatg aaaagccgct
     7261 agcccggcgg ctttttccag aataggacgc ggcaagctcg ccaccttgcc gggattacga
     7321 acaattatat gcgatccact cgtgccctga gcatgaagcc agaagtcgtt cggtcgagcg
     7381 aatttctgga gaagttcgtc gttatcacgg gcgctcttgc cgattaaaat ttcccagccg
     7441 tccaaattga tattaataaa gggctgatgg atattttcaa tcgagctttg cggctgagcc
     7501 ggcaaatgag tggacaaagt tcggagagtt ttgaagtcgt cagcctcggc gatttgtttt
     7561 tgggtatttt ccagccgctg aattaactgt tccgcttcgg caattttttg aatcaattca
     7621 gcccttgctt tttcgatttg acgtgatttg gcgtaatacc gttcggcatt ttgaatcgga
     7681 ttgagttttg gttcaagcgg aatggtaatc tggcgggctt catcggttaa ttgaggcaag
     7741 gttaccgaat tgctatgatg cggtatggtg gacgccgtcg ccataagggt atcagcccat
     7801 tcccgataat tatccgccgt cggtagagcc gccagttcgc tttgttgctt gatcaatttt
     7861 ctctgggtag tgctcagcag aaactcggtt tgtctcgaca aagttttttt cttttccttc
     7921 aagcgataga ggttcaggta atcctgaata aattggtttt gagcggcgat aactgagtcg
     7981 tactcggcga attcgcgatt tgaaagcgat tgcagttcaa ttaaactaaa aatcggcggc
     8041 tcgccgctat aaattcgcgg caaagaagtt acaattgcat ctgccaattc gtctatagtt
     8101 gtggcgagtc tttcaacctg ttcgtccgtc aaattgataa ttgaatactg cggcgccaga
     8161 ttagttcgat agcaaatttc ggtcacgaga attttcggta aaatcggttg aaatgatttg
     8221 gtgaaaaaag tcatcagtga ctgatcggaa tgctgacgca atgaactggt aaaatcggca
     8281 taatcgggaa aaaggcgatt ggcaggtcgg aaattgctca actcaaagtt taccagcttt
     8341 tttgcttttt taaatgaatc ccgaatcacg ccggatgaat cgagaagata tacatttcca
     8401 ttgattccat agagatggaa tagaaggtag gcgatcccac tatttaaagt aattaaaacc
     8461 tggcggtcgt tttcacggca aaccaaatcg gtaattgctt ttgcggttac gtcacgaagc
     8521 agattgacct tgtgttttgg ttgcggcaaa gtcctttcta aagtaaaaaa tggtaggggc
     8581 gcttgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgtgt tttggttgcg
     8701 gcaaagtcct ttctaaagta aaaaatggta ggggcgcttg aacgctaaaa tgcaggcatt
     8761 cccattcacc cgccatttcc agcggaatat gcaaatgatc cttttgatag gtaaagggca
     8821 ttccacaaat tgcaccgccg atcaattgtt ggtattcgcg tgccaatctt tcaatatgta
     8881 gccaactgtt aaacatattg aaaagtagaa ctaaaagagc atttatcaaa gcaccaagcg
     8941 gatgttgttt ggttggcaac cggttacagc tatatagttc gtgaggcgct gataaattcc
     9001 acttggtttc ttcataaata aaaataggtt gcaatttaat ataaaataaa gattaaatgg
     9061 cgattttaaa tttgacagtt tggactggat taactaattt taccattgat ttgataggag
     9121 atttgcaatt ggaaagtatg acaggttata gtagaacgga gtaccgtagc ggcggtcaag
     9181 taatcagcgt tgagatttac tccgaaaata accgctttct agacctgact ttcaatcttc
     9241 ccgaaccgct cgaaccatac actgaggagt tgaagcgctt ggtagctggc gatctgcggc
     9301 gcggtcgagt ccgcatcact atcgcaatta ccggttcaga aaaaaccccc gaggtgcaat
     9361 taaatactgc ttttttgacc aaatattacc gagcgctatg tgatttagca cgcgaattgc
     9421 aaatcgaaga gccggttact cttcaaaatt tgcttaccta tccggaaatt tttatgaccg
     9481 attccgcgga tactgatgtt gctgaacttt ttactcaggt aaaagacctt ctgcgtcagg
     9541 cggtggctga gctaatagct atgcgtcgcc gggaaggcga atttattacc cgagaattac
     9601 agagccattt gcaatttatc ttggaaaaat gttcggagat tgaaaaactc tcccagcaat
     9661 cgcgccagga atatttcacg aaataccagc agagtctggc ggagctgtgc caaaattgtc
     9721 aacttgatga aaatcgcttg attcaagaag ccggaatttt agccaaaaaa ttggacatta
     9781 ctgaggaatg cacccgtctg cgtagtcaca ttcagcagtt taccttacta ttacaaagcg
     9841 aagaaccggt cggcaagcag atgaattttt tagcacagga aatgaatcga gagattacta
     9901 ccattgggtc aaaatcagaa agtgccgcaa tttctcatct ggtcgtcaac ctcaaaaatg
     9961 agcttgaaaa aattcgcgaa caaatccaga acgtaatatg aaacccaaag gacttttagt
    10021 agctattgcc gctccatcgg gaacaggtaa gaccacagtt tgccatgaac taatgaaaag
    10081 ggatgaccgt tttgtttttt ccgtttcctg taccactcgt ccaccccgtc cgaatgaacg
    10141 cgacggcgta gattatcatt tcattagtcg tgagcaattt ctgaaaaata ttcacaaagg
    10201 taaaatggct gaatggcagg agattttcgg caattactac ggaactttaa aagattcaat
    10261 cgaacaagcc ttgagagatg gcaaaatttt acttttagat ctcgatgtaa aaggcacact
    10321 caatttgaaa aaaaattacc ccgaccagct aattagcatc ttccttttac cacccagcga
    10381 tacggaactc gaccggcgtc tccagaaacg tgggacggat gactcgagtt ctatcgctat
    10441 acggaaagcc cgaatcccag atgaattgga actaagtgaa cagttcgact ttcaaattgt
    10501 caatgataat ctggaagcca ccgtcaataa aattattaat atcatagagg agcatcttaa
    10561 aaaatgactg aaacccttcc ttttagcaaa ctaactcgga aatcagacga tattttcgaa
    10621 ctggtggttg ttgccgccaa gcgcgcgcgt cagattaacg ccttgcgtat ggctcaatat
    10681 cccttgccga cgatggttga ggaccaagag gagactttcg aagaaactcc accggaaatc
    10741 accgacgaac cggcttggga ctatctcgaa aagccgacca cattagccat caacgaaatt
    10801 ttagaaggta aaatcaacta tcgctatatg acccccgaag aagaaaaacc gcttgaggaa
    10861 ttcgatagtg aatttagcga atagaaaaat cgttctcggt ttaaccggcg gcatttccat
    10921 ttataaatcg gctgggcttt tgcgccgtct ggtcaatgac tataaggcgg atgttaccgt
    10981 gattatgacc gacgccgccc aaaagtttat gagcccgctg atttttgaaa ccttcagcca
    11041 caaacccgtt ttaaccgata tgtttaccgg tcagaaaatt gaaacccgcc atattgatct
    11101 ggcgactgaa gccgatttga ttctggtgtc tccggctact gccaatattg tcgctaaagc
    11161 tgccaatggt atcgctgatg atttgcttag tacaattatt ctggtcggcg ggaacaaaac
    11221 ggtttttgcc ctggcaatga acgataatat gtatgctaat ccgattactc aggagaatat
    11281 cagccgtcta cggcgactcg gttacgacat catcgaaccg gaggaaggcg ccttagcctg
    11341 tggtagcacc gggcgcggac gcctggctga cgagacgaca attctggcgc atattgaccg
    11401 ccaccttaat ggcaataaat ggctgaaaga taaaaaagtt atcgtaacgg ctgcccctac
    11461 tcgcgaatat attgatccga ttcggtttat ctccaatcgg tcgagtggca aaatgggcta
    11521 tgccctagcg gaaattgcat tccgcgaaag tgccgatgta cttctaatta gtggaccttc
    11581 tgatttaaaa aatacggtgg gcgtaaagac aattcgagtt gagactgcga acgaaatgcg
    11641 ttccgccgtc ctaagtgaag cggctgaagc tgattattta tttatggcgg cggctgtcga
    11701 agatttatca cccgtgcaaa ttgccactca gaagattaaa aaagagagtg gcattagcaa
    11761 tctaaagctt aaatattcgc ctgatattat catggatttt cgcaaacata atcgtcaggc
    11821 atgcgtggtc ggattcagcg tcgagattga atccgccgag catcctggtg ccaaggcgcg
    11881 ttcattagcc aaactgaacg acaaagagat ggattttatc gtctggaatg atcccaacgt
    11941 gaccggtgcg gctttcgggc atgaaaccaa tagtgttact ttattatcaa aagatggtca
    12001 agaatgggtt tttcctttag ccagtaaacg tgacatcgcc gattcaatca tttcgactgt
    12061 tattaaaaat tataaaaaag cttaaatgac cagactatac gacgatatca aaacattttt
    12121 caaacaacag gctgaattat ttccccccga aatctattta atttccgaaa atggcgagga
    12181 atctacaccg aaaactgacg ccgccaccca gctagcggaa ttggagcgca tttgtcaaaa
    12241 ctgccgacaa tgtcatctat ccgaaactcg cactaaggtg gtttttggaa ccggtaatcc
    12301 tcatgctaaa ctgctattta ttggtgaagc accgggagtt aatgaagatt tgcaaggatt
    12361 accgtttgtg ggaagagctg gtaatttgct cgataaaatg ttaaaagcga ttggcttgac
    12421 ccgcgctcag gtttatattg ccaatgtttt gaaatgccgt ccaccagaaa atcgtactcc
    12481 taacgttggt gaggttgaaa agtgtgagcc ttatcttttg aaacaaatcg caataattca
    12541 gcctgaattg ataatatgtc tcggtttaac ctcagctaaa actttattac gagtcgaata
    12601 tactctggag aaaatgcgcg gtcagatttt tcagtatcac aatgtggatt taatcgtgac
    12661 ttaccatccg gcagcccttt tgcgcaatga gaatttgaaa aaatatgcct gggaagattt
    12721 caagaaaatc aagaacttat acttggataa aacaggagaa taaatggctt taaatgacgg
    12781 tgaaataatc agagtccctc cgcattccga cgaagccgaa gtttccgtgc tcggggcgat
    12841 gatgctcgat aaagaggcgg ttagtaaagc tctccagtac ctcgattata ccgctttcta
    12901 taaagaagct caccagcata tttttcaagc tatgactgac ctctttaatg aaggacagcc
    12961 ggttgatcag gttagcgtta tagaccagct caaacgcaaa aaaatgctgg agcaggtcgg
    13021 tggcgcttat tatgtcaccg gactggttga ggctactcct tcagccgcta atgttgaata
    13081 ttatgccaat atcgtcctcg atatggcaat tttacgtcgc ctaatcgtgg cttctaatga
    13141 aatccaaacc gaatcttatg aagctcgcga ttcggtgcag gatattttag ataccgccga
    13201 acaaaagata tttgcgattt ccgaaaaccg gatgaaaggc ggctttaaaa atttcgtgga
    13261 cgttctcaat cgcacatttg aacacattga ttcgattcac agtcggaaag ggcatacgag
    13321 tggcgtgccg actggactct tggatttaga tgaattgacc tccggctttc atccgggaga
    13381 attgataatt ctggctggcc gaccgggtat gggcaaaacc gctttggctt tgacaattgc
    13441 acgcaatgcc gccgtagaat ataatatgcc ggtcgctttc tttagtcttg aaatggctga
    13501 ttaccagctg gctttacgaa tcctatgtgc cgaagctaga ctggatagcc atttagtacg
    13561 taaaggtaaa ttgccgaagg agcaatggca aagactaagt atgcagactg ggcgtttagc
    13621 caacgctaaa atctatattg atgattcgcc ggtgctgaca atgatggata tccgcgccaa
    13681 agcccgacgt gccaaagccg agcacgacat ccagctggtg attatagatt atttgcaact
    13741 cattaagggc catcgggccg aaaatcgcca gcaggaaatc accgagattt cacggggact
    13801 gaaagcctta tctaaagaaa tcggtgtacc agtcatcgct ctttcgcaat tatcacgagc
    13861 ggtcgaaggc cgcgccgatc accgaccgca actatccgat ctgcgggaaa gcggttcgct
    13921 ggaacaggat gccgatgttg tgatcttcat ttatcgcaaa tcgttttacg ataagcgtga
    13981 aggtagtgcc gcatcggacg aagatgaacg tctcgccgaa atcatagtcg ctaaacaacg
    14041 caatggccct actgacaccg tcgaagttat ctttattgat aaatatactc gctttgaaaa
    14101 taagggtgcc caaagagaag aaatcataac aactccattt taatggcaac tgaaatactc
    14161 aataaagtcc taccgcgcgc gaaacagcgt ccccagaaat tccttactct gatcagtaag
    14221 ttgaatcagc gcgcgccttt aactaaagat gaggagaatt ttctttggga agtatatagt
    14281 tttgcacgca actcacaccg cggtcaacgt cgtcaatccg gggatagttt ttttgaacat
    14341 ccttatcaca ctgctctgat tttggcggat ctgcatatgg atccaataac ggttgccggt
    14401 ggattattac acgatgtcat cgaagatact gaggttacag taaaagatat tgctgagaaa
    14461 tttggggatg atgtcgccaa attggtcgag ggtgttacca aaattagcgg aattaccttt
    14521 cgcaatcggc aagaagaaca ggctgataat tttcgcaaaa tgcttttgag cgttgctgaa
    14581 gacatccggg tgctaatcat caaattggct gatcgtctgc ataatatgca aacgctcgat
    14641 tctctgccac cgattaaaca acgtcgggtt gcgatcgaaa ctcgcgatgt atatgctccg
    14701 cttgcccatc gtctggggat gttcaagtta aaatccgaaa tggaagacct ggttctgaaa
    14761 gccctggatc cggatgctta ccgatttctc gaaaaaaagg ttaacgaaaa acgcagtgaa
    14821 cgcgaaaaat atattaaagt cttcacccag ccaattatta aggctttgaa cgaacagggc
    14881 attaaagcca agattgtcgg tcgcccgaag aatttttatt cgatttataa gaagatgaaa
    14941 actcaaaata aaccttttga ggaaatcttt gacctgcttg cagtccggat aatagttgat
    15001 actaaagaaa attgctatac ggtgctggga attgttcacg atctttttac gccggttatg
    15061 gaccggttca aggattatat cgccaaccac aaagccaact tctaccaatc cattcacact
    15121 acggtttacg gtcccagtgg tcgtatggtg gaaattcaaa tccggacaac cgaaatggat
    15181 gagatcgccg aagagggtat cgccgctcat tggcgttata aggaaggtcg cgacaaaccg
    15241 gacgacattg ataaatatgt caaatggtta cgcgatttaa tcgagatcct gcagaccgaa
    15301 tctaccggcg ccaaagactt tatggatacc cttaagattg atctttttaa agatgagatt
    15361 ttcgtcttca cgccgatggg cgatctgatt cgcttaccca agggggccac tccggtggat
    15421 tttgcctatg aagtccatac tgaaattgga acacgctgta tcggggctaa agttgacggc
    15481 aagatggtac cgctaaatag cgaattgcgc agtggccaaa ccattgaaat cattacttcc
    15541 gattctcacc gacccagtta tgcctggttg aaatttgtca cgactgccaa agcaattgga
    15601 gcaatcaagc gttatttacg caaagctcaa ttcgaagaaa gtatcaatct cgggaaggaa
    15661 attctggaaa aagaaaacca gcgcaataaa gaattgcgat ttttgaaaac tgtgcaggaa
    15721 tcgttcgaag aactcggatt tagtgatctt aataaactat atgcggcggt cggtagtggt
    15781 gttctaacca tcccccagat ttatgctaaa ctctttcctc agaagcaaga gcagctggtt
    15841 cgtcaggacc tcgatgaact ttttatcgaa acggctcgca aatcggtcaa aggcatcaag
    15901 gtgcaaggta taagtaactt gatggtttcg tttgccaaat gttgtaaccc tattccaggt
    15961 gaccagatca tcggctttgt cagtcgcggg cggggggtga taatccatcg gagtgactgt
    16021 tcgaatattc cttctctatt agaagaggat gagcgtctgc tggacgtgga atgggatatc
    16081 gcgggaacgc aagccttttt agtcagattg aaaataatgg ctcaggaacg caagaaattt
    16141 ctgaaggatg ttaccgatat aatggcaagt accgatacga atattattag cgtgagtggt
    16201 gacgttgatg aatcaattat tcatttgaat ctggttatcc aggtcgaaaa tctgcgtcat
    16261 ctcaataaaa ttttagcgaa attgcaaaat gtgcagggta taatttcagt agaacgtaaa
    16321 taaacaaaag gagattagaa aatgaaagta aaaacttaca ccaaaaaaga tgtcgtcaga
    16381 cgcacagctg aagcgcttga taccaaattg gttgacactg ccgagattgt ggacggagtc
    16441 tttatggttc tgcgcgaaat gctcagtgaa tctgacccga gaatccggat tgaaattcgt
    16501 aattttggtg tttttgaagt caaaccgact aaagccaaac ccaaagcccg caatccgcgc
    16561 actaacgaag aagtatatgt tcctgctcgc cgcaaaactc atttcaaacc cggtcggcta
    16621 ctgaaagaaa ggatgaagaa agcgtaaaag acgccgaaaa agtacttcac ctttataaaa
    16681 aactctgagc caaagatgat gaaagtcaaa atcaaccttt ggtggttgat attaatttct
    16741 ggtgtattaa gtggattggc ttttccgccc ttgccgctgg gatttttggc ttttttcgcc
    16801 ttggttcctt tgattaaagc tttttgtgat gatgaatttc ataatggttt tgaaaaagga
    16861 ttggtttatg ggctggtgct gaatttggcg attatgtact ggttggcgtt aaataaagga
    16921 actacctggt actgggcgac tttatctatg gtagcggctg ttatttttct ggctatgaat
    16981 tatgctatca tcggtttgag cgttggcttt atcggccggc gactgggacg gaacgtcggc
    17041 atctggtcat ttccgattat ctggactgct atcgaatatc tgcgttcctt tggctcattg
    17101 ggttttacct ggaataatct gtgttacact cagtcgcatg ccattcagtt aatccaatcg
    17161 gcggctgtgg ttggtcctta tggaatttcg ggctggatag tggtcattaa tgtccttatt
    17221 tttcgcttgc tggaaaatct cgaagattac cgtcaggtga gtcggcgcat ggtgataatt
    17281 atcgccctat ttttaattcc agcaatctat ggaatggtcg ttttgcaaaa accatcagac
    17341 gaaaacgacc ggcgtttagt caatgtcggc ttgattcaac cgaatattga tcccaacgcc
    17401 aaatgggatc aggaatcttt caaaaaaaat atgcaattgt tgcacgactt aactgattcg
    17461 gtggcaaccc agccgctgga cctggtagtt tggcctgaga ctgctacgcc gacttttttg
    17521 cgacggaatc attatgccgt tttgactgaa ataagagagc atatcgaaca actgcagatt
    17581 aatctattga ccggtactcc ggatctggaa cgtgcgggag aagattatcg gtattacaat
    17641 gcgacttttc tgctccgtcc gcattccaac cggattgaag attatcgcaa aattcatctt
    17701 gtcccgtttg gcgaatatat cccgctttct ggctattttc cggaattgag taatcttaat
    17761 ctcgggcaag gcaattttca ggctggaaaa gaggttaagt tattccagat tccccttaga
    17821 gttgaccaaa ggccggtctc cgatacggtt ttgactttta attccgtgat ttgttacgaa
    17881 tcaactttcc cagatctggt tcgtcaagct acccgccagg gctcggaatt gctggtgatt
    17941 gtttccaacg atgcctggtt tgggaatact tcggcaccct ttctccatgc tgagattggg
    18001 cgttggcggg ctatcgagaa tcgtattccg gttgtccgtt ctgccaatac cggaatttca
    18061 cttattttag accaatatgg caggaatctg caatccttgc cttttggcaa atcggggtgg
    18121 ctttcagcgc cagttactca gggaaatcca caaactattt ttgtgagata tgggaactgg
    18181 ccagcgcgct tatgtgttat actggcattg ttgttaatcg gctattcaat aataagaagg
    18241 aaaaaatgaa gcatttattt tgcccgagtc tcttgggatt attaataatt tttattcttg
    18301 caggaagcgg tacagctcag attgattcta cgcttgtcgc tcaacctgat tcacttcccc
    18361 aattgactca aagtcaggcg cgtctgctat tattaaaatc ggctgttctt cccggctggg
    18421 gtgaacgcag tttgaattac gttgaacgta gtcattattt taatgcctcc gaaatagctc
    18481 tctgggtgac ttattttgct ttttattggt atggaaacgc ggttgccgct gatatggaag
    18541 cctatgccgc cacgcatgcc ggagtgaatc cagacggcaa agatcaatat tatttcaccg
    18601 atataggcaa ttatgaaaat atctacgcct ataatgaaca gaaattgcgc taccgggcgg
    18661 ttaatttgct ctatccggtg actcccgaat attactgggc gtgggacagt gaatcttctc
    18721 gccgtcactt tgacaaacta cgagtgaaaa gcgctacagc tttacggaat gcgacttttg
    18781 ctatcggcgg cttggtagcc aaccgactct ttagcatgct ggatgtaata gttctgacgc
    18841 gcaaccgatt ggaaatgccg gatatagata ttgaatccaa ctggcaacca tatcaggatg
    18901 gcatgtcttt tatgctgagt cttaaattct aaaatgaaaa accttgatcc tcgggtaaaa
    18961 attctctggc tgatgatagc cgtaataggt attttccgcc tcaaagtttg gtatgcccag
    19021 cttggagtcg ctcttgtatt gggaactata ataattttcc gcactatttc tacgaaggag
    19081 agtttcaaag ttctcagaat tctgttggta ttcttaccgt tgacattttt gatccacatt
    19141 ttgtttggaa cgaacttgct agccactatt ttccgttcag ctcctcgtga ttcctggtcg
    19201 gttttactat ttacgccgct gacctttaca ttgaggatcg gtaatttgat agctctgatg
    19261 gctctcgcga ctcattggtt aaaggcgctg gaggtactgg atgctctata ccatttacta
    19321 aagcccttga gaaaaatcgg tttgccagta gatgatattt ttcagataat ttttatcgca
    19381 gtgcgcttta ttccactaat tcaagaagag tatcaaaggc tcgacgatag ctggcgtatt
    19441 tttatgaacc gcgattcagg tggtctattt ggtcaaattg agcgcctgcg aacgattctg
    19501 gttccgttaa tggtggttag ctttcgacgg gcggaagtat tagccgaggc aatgacggta
    19561 cgcggttata attctcaagt taagcgctcc tacatcggcc gattacattg gcaaacacct
    19621 gattggatgg gattagtggc tggttgcggg agcttggttc tgatggttat taatatctga
    19681 tgggacgtcg ctttaaaatt attatgcagt atgacggaag caattattgt ggctggcaac
    19741 gtcaacgtta tcagccttcc gtgcaggcga aaattgaaaa tgctctgcaa cctctgaatc
    19801 agaatcagcc ggtgatggtg attgccgctg gtcgtacaga tagcggtgtg catgccaggc
    19861 gtcagaccgc ccatttcgat ttggtgacgg atttatcacc agaaactatc agaaaagcct
    19921 tgaatgcaac tttgccggat gatatctaca tagactattg ccaggaggta gggaaggaat
    19981 ttcacgctcg ttttaccgcg attaatcgta cctatcacta ccaggtggtt gttaacccta
    20041 acgtattttt acgcaaatat gtctggctgg tcaattttcg ttttgatctt gagttgttgc
    20101 aggagtgtgc cgaattggtg aagggtgaac acgatttcac agcactatgc cgggcggcga
    20161 ccgagacgga aaataaaatg gcgacgattt acgaatcgcg ctgggaagaa caggacgaac
    20221 tattagttta tatcataacc gccaatcgct ttttacatgc aatggtcagg atgctggtgg
    20281 gttcaatgat ggaagttgcc agaggaaaat attcgatcgg tgctttcaag aatcttcttg
    20341 gaaaaaatga tactaaaatg caagtttata cagctccggc gcaggggctg tttttatgga
    20401 atgttaaata tcaggaggac aattaaaaat gaaactgttt attgacactg ctaatctcga
    20461 agaaattaga aaagctcagt cctggggact attggatggt gttaccacta atccgacttt
    20521 gttatccaag gagaagggca attttaaaga gattcttctc aaaatatgcg agaccgtcaa
    20581 tggaccggta agtggcgaag ttgtcagttt agatgctgaa ggcatggtgc gcgaagctga
    20641 ggatttagcc gctttgcata aaaatatggt catcaaggtt ccgattactt tggaaggatt
    20701 aaaggctatc aaaattttat ccgagaaggg aattcgtact aatacaaccc tcattttttc
    20761 gcctttgcag gcgattctgg cggcgaaagc cggggcaact tttatcagtc ccttcgtcgg
    20821 gcgtctggat gatattcatc aggtcgggat ggaattagtt cagcagatta agactattta
    20881 tgacaattat ggtatcagga cggaaatcat tgtagccagt attcgcaatc cgttgcatat
    20941 tgtggaagcg gctctaatcg gggcggatat ttgtacaatt cctttcaatg ttatggaaaa
    21001 aatggtcaaa catcctctaa cagataaggg catcacccaa tttctggagg attggaaaaa
    21061 ggtcgaaaag tgaaaaaaat aaattcggtg ttaattagca gtttgtttat cggcgtaagc
    21121 cttttactgg ctgttccaca agaacaacaa gaaatcagta attcacgccg caatgccata
    21181 actgcggcca tcgaaaaagt cagcccggct gtggcgggta ttaacgtcac ggcaattgag
    21241 gagtatgtag cctctccgtt tttcaatgac ccgctctgga gtttgctgtt tcccgagcag
    21301 atatatcgtc gtcgagtaaa aagcatcggt tcgggcgtag taatcagttc tgatggttat
    21361 atcgttacca atactcatgt agtcgaaggt gccgaagaga ttgttgtgac tttgatgggt
    21421 ggcaaagaat ataaggctaa attagtcggg attgataata tttccgatat cgctttgctt
    21481 aagattgatg gcaagtcttt tacgtatgcc aaactgggtt cttcggaaga cgtaattatt
    21541 ggcgaatggg taattgcact cggtaatcct tttggccttt ttagcgttaa ttataagccg
    21601 acagctacga tcgggatagt ttcgggaacc gacctcgatt ttgggcctca acagagcggt
    21661 cgcctatacc aggacatgat tcaaaccgat gcggcgatta ataccggcaa cagtggtgga
    21721 ccattagtaa atgcgctggg cgaagtcatt ggcatcaaca catttatttt caccggtggt
    21781 caatttagtg agggctcgat cggaatcggc tttgctattc cggttgaccg ggtaaaggcg
    21841 gttgtagcta atcttaagca gtacggtaag gacagccgaa cttttaaaat tggctgggag
    21901 cttcagaatg tggatagttt tttagcgagt tatttaaatt taccgccgaa taccggtgta
    21961 attgtcacac gagtggaaaa aagtactcca gccgaacgct ctgggctgaa agtcggcgac
    22021 gttattatat cgattaacgg gaaaaaggtt gccagggcgg aagaaatata tgcttacatc
    22081 caggaaaatt tgctaaggca aggcgataag attaaagtcc gatttttacg tggtaatcgt
    22141 gaatatgaaa cccagctagt tttagaatag ccaatgatag aacgctatac gcgacctgca
    22201 atgggacgaa tctggtcgga agagaataaa ttggcgacct ggtggagagt tgagctggaa
    22261 gtttgtcggg ttcaaaatga gcgggggatt atccccgacg aagccttgca ggaaattgaa
    22321 tcgcgggcaa aattttcatt aaaacgcatt caagaaattg aggaagaagt caaacacgat
    22381 gttatcgctt ttttgactaa tatcagtgaa aatgttggcc cagccgcccg cttcattcac
    22441 aaagggatga cttcttcaga tttactcgat actgctctgg cgcttcaatt aagcgaagca
    22501 ggccaattga ttctcgctga cctgaaccgg ttggagaaaa ttttaactga aaaaagtatt
    22561 gagtacaaag attgtttaat gatcgggcgt actcacggtg tccatgctga gccgattact
    22621 tttggattta aattgctcat ttttcgcgag gaaatcaggc gccatattaa gcgatttgag
    22681 ctggcactgg atgatattcg agttgggaaa atttccggag ccgtcggtac ctaccagcac
    22741 attgagccgg aagttgaaga acgtgtctgc cacaatctcg ggctggaacc cgctccagtc
    22801 actaaccaga ttatccagcg tgaccgccat gcctttttca ttgctactct ggcattgatc
    22861 ggaacgactc tggaaaaaat tgcgaccgaa atcaggcact tacaacgcac agaggttctg
    22921 gaggttgagg aatttttctc agaagggcag aagggttcat ctgctatgcc gcacaagcgt
    22981 aatccggtgc tttccgagcg aatttgtgga atggcacgac tcctgcgcgg ctatgccgtg
    23041 accgctatgg aaaatattcc gctctggcat gaacgcgata tctcacattc ctccaacgag
    23101 cgagtcatcc tgccagacgc ctgtatttgt actgatttca ttctcaatga aatgagtaaa
    23161 gtgctaacaa atctcctcgt ttaccctgaa aatatgcgcc gtaatctcga aggaatgcac
    23221 ggattaatct tttcacaaga gattatgctg gcaataattg aaaaaggtgc aagtcgtgaa
    23281 gaggcttata ccattgtcca gaataatgcg atgaaggttt ggcaatcggg ctccgatttt
    23341 aaggaattgc ttttgaaaga tgagaatgtt ctcaaatggt taaatccaac cgaaattgag
    23401 tcttatttta attacgaaaa aattttaact cgaatcaata gaatttttga acggatagac
    23461 tcaggtaatt atgaaaaaga atgaaaaatt atacgaaggg aaagccaaaa tcgtctattc
    23521 tactgatgat ccggagttag tcattcagtt ttttaaagat gacgccactg cctttgatgg
    23581 cattaaaaaa ggacagattg gcggtaaagg agtagcgaac aatctaatca gttgccatct
    23641 atttagcctt ttagaaaata acggaattgc tactcatttt atcaaaaaac tctcggataa
    23701 cgagatgtta gtacggcggg ttaaaattat ccccgtcgaa ttagtagtac gtaacgtggc
    23761 ggctggcagt ctttgcaaac gttatggagt accggaaggg caggttttca gtgagccaat
    23821 tgtagaatgt tattataaaa acgatgcctt tcatgatcca gcaatgaatg acgaccatat
    23881 catccggttt aatctggcta atgctggcga attatcggct atcaaagcca ccgcactgcg
    23941 aatcaatcaa attctgaaag aatattttga ttcaatcggg ataatcctgg tggattttaa
    24001 gctggaattt ggtcgttata atgaccagat tctactagct gacgaaattt caccggatac
    24061 ttgccgcttc tgggaaaaag gcactaaccg gaaactagac aaagaccgct ttcggtttga
    24121 tttgggcgag gtggaggaag cctataccga gatgttaaag cggattacag ggattcaaaa
    24181 atgattgcca aagaaagcct gcgcctgatt ataccgatgt tgattgttgc tgttctggcg
    24241 atttttttgg ctaaatattt tgatttgggg attatgatgg tgcttttctg ggtgacatta
    24301 gtgagtataa tctttacgct ctattttttc cgcgatccgg agcgaatcac tcctcagaat
    24361 ccggcatttc taattgcgcc tgcagatgga aaagtcgtag gaatcagcga agtaaatgac
    24421 ccttttgtcg gcggcgggat taaaattgcc atttttatgt cgccttttga cgtgcatatt
    24481 aaccgcgcac cctattcagg gattataagg caaattgaac ataagcgtgg taaatttcac
    24541 tcggctttta agccggaagc ggcgattgag aacgaacaat gccggataac gatggaaaca
    24601 gataacctca aacttgctgt gactcagata gccggttttt gtgcgcggcg gattatttcg
    24661 cgggtgaaag tcagccagaa tttagagaaa ggcgagcgat ttggtttgat aatgtttgga
    24721 tcgcgggtag aattaataat tccgagcaaa ctgaaaatta acatttgtcc gggagcgaaa
    24781 gtcaaagccg gagaaaccgt gattggagaa atataaaggt gagacgcaat ttgcttccaa
    24841 gtatcttgac tattttgaac ctgtttgccg gatttttggc tttactccag ataagccagg
    24901 ggcattatat tgcggcggtg gaattgatga tcgtcgctct catttttgat ggtttggatg
    24961 gaaaaattgc ccgtttcact catcaaacgt ctgattttgg gatcgaattc gattcgcttt
    25021 gtgatatggt ttctttttgt ttggtgccag cagtcttaat ccacgaacta tttgtatccg
    25081 agttaggatt tattggcggc ttgatcagct tttttccgct tttatttggc ggcgtccggc
    25141 tggcgcgctt taatctgcag gctggtgtgg gcaagaaaag ctatttcatt ggaatgccaa
    25201 ccccgaccaa cgctctggcg ataggctctt atatctggtt tagttataaa gtattaggta
    25261 attatggtaa tccgaagata atccttccac tggtggtagt attatcgttt atgatggtaa
    25321 gtaatattcg ctttagcgtt aatctcaaac ttggttttcg tcgcggcttg ccaactgcgt
    25381 ttaaaaccgg gatgataatt cttataacga tcgggatggt aattttcaat ggatatgtta
    25441 tatttccgtt ggtgatggtt tatataatta ctcatctgtt gcaatggtta atcggctatg
    25501 atgaaccccg tattcattta gccagaagaa aggcgcatta aatgtggt
//
LOCUS       DAOSMP010000032        25091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_1990_length_25091_cov_22.415122, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000032 DAOSMP010000000
VERSION     DAOSMP010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 25091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25091
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1990_length_25091_cov_22.415122"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..998)
                     /locus_tag="PKX09_06655"
     CDS             complement(72..998)
                     /locus_tag="PKX09_06655"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOU17537.1"
                     /translation="MPRKSASFYFKESILLVIKLALSIVLVNIAIRKINLADLRWIFS
                     TISWSRILFLCVLIMFNWAFQIIRWGIILKEYSESAKWHETIKSFFIGHTFRLVIPGG
                     YAEFLKIYYLNGKHRHGFMAYLVELSSVMLTQIILLVWAGLHLFPHHKWIFISIGIVT
                     IGACAGFPYLKKIRFVKKYLPNPTIRWSLILKSGILTVFSLILISSEYHFIINWAGSI
                     SWSAAAQSVIFILFANSVPFTFGGLGVRENVAVYLFGLFGITAPVAVATSLFVFVTNS
                     LMPAFIGLVLMLLHRVVRRTPVSPVQPISSKA"
     gene            complement(1005..2099)
                     /locus_tag="PKX09_06660"
     CDS             complement(1005..2099)
                     /locus_tag="PKX09_06660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU17538.1"
                     /translation="MLNLIEFAILLTFFYLIIMGVVGIGLIFIRTQKRVFQTLAVSVI
                     VCARNEEQIISRLLQSLTNLRYPEDKYEIILVDDDSTDDTLSIMNDFASGKSNWKVLH
                     HDKTTDQLKGKKGAITLGVQNSCGEIILVTDADCVVPPNWIASMVSYFRKEVGMVLGH
                     SPVARRKGFMNIYERFDTLCEASVAAASTFYNKPTHANGRNLAYRKKAFEDVNGYYRS
                     ANTDTGDDFFLMQEIRTRTRWRFAYNIDPASYVTTEPTKFGKKYIHQQLRRNSKAFNL
                     TIPFFILGVIVFLFHVVLAIMLFSPSLWNWLGMMLFFKFMAEFIAVYHGAHLFHQTDL
                     LKYFPILWVIYPVCYLLSQVIGSLKIYRWK"
     gene            complement(2099..3778)
                     /locus_tag="PKX09_06665"
     CDS             complement(2099..3778)
                     /locus_tag="PKX09_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17539.1"
                     /translation="MRNIRLVNLTFIIITAFPLVISAIGIDVPATDPVYDFLKRLETR
                     GIIKGFNDLTLPLTRDEIVNFLQIASQKADKLSVIEKNILREFLADYRYETERQLTEP
                     DSISAPFYQNNYFRQATANLFSRQDAVEENHLLMYESGETFFWFDIGVRGRMDWKENH
                     QKQMFSDRYLLRGALSKSLTFHTYFARFMKNGDPAFTEPYEEEIGNFSMVQPGGTVTF
                     DILQTSLVFHQKYFNIGLYRQPVAWGASGTNNLILSKSAPLFSYIGFDTKFKGIKLSM
                     IHGSLLNDSTHYRDIALQQRNRSKYIAAHRLDIPFFRGTTTVGFSEMVIYGDREIDIA
                     YMTPFGFYWPIGHALEDRDNTLMAVDFKTTFIKNITFYGSVLIDEMRFSELGKQWWAN
                     KHALQAGMRWSPNIATLPFDFQAEFTAVRPWTYSHKTLTTNYTHNSICLGFPYGANSQ
                     TWYFKIGTALSRRLTLTSEYLILKKGVDDSTHFWGGDATVSYELRDEQYDHATKWLMG
                     DIETTNQLKVAGRYEIFNDFFIKAGINLYNTKLNNDQESYRFGFIETEINF"
     gene            complement(3808..4581)
                     /locus_tag="PKX09_06670"
     CDS             complement(3808..4581)
                     /locus_tag="PKX09_06670"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020029636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOU17540.1"
                     /translation="MKEKIEIARSWLPRYTGLPLEKYGDYILLTNFRNYVDIFCTMFS
                     CDLYGENRPMQAATNSAGLTIINSGIGSPNAATIMDLLVAVHPKGVLFLGKCGGLKKS
                     TEIGHFILPIAAIRGEGTSDDYFPPQVPALPSFKLHKFVSEIIVQRGLEYRTGVVYTT
                     NRRVWEHDQAFHQRLREMTCIAIDMETATLFIVGHYNEIARGALLLVSDLPTTPEGIK
                     TEESDKQVTAQWSQIHLQIGIDAMTNIGRMGEPIKHFRY"
     gene            complement(4595..5005)
                     /locus_tag="PKX09_06675"
     CDS             complement(4595..5005)
                     /locus_tag="PKX09_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU17541.1"
                     /translation="MRSWTLPALIALILWGFWGFLPKVATRYIDPSSALVYQAIGGTI
                     VGLGVLLSVRSQIQWQAVGAFWAILTGLFGMLGSLFFLQAVSRGKVTTIVTMTALYPL
                     ISIVLALIFLKEPFSFRQGLGVFCALMALILFSI"
     gene            complement(5139..5477)
                     /locus_tag="PKX09_06680"
     CDS             complement(5139..5477)
                     /locus_tag="PKX09_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOU17542.1"
                     /translation="MKIAIPLANGKLTLHFGHCEQFALVDVEVQNKKIIKRTDLIPPP
                     HEPGLLPSWLAEHGVNLIIAGGMGQRALMLFAENNIQVLVGACSESPEKLIGDYFDGN
                     LQLTSNVCDH"
     gene            complement(5496..6404)
                     /locus_tag="PKX09_06685"
     CDS             complement(5496..6404)
                     /locus_tag="PKX09_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOU17543.1"
                     /translation="MSDISKEKTKSTCDSCGNSTCSAAKPREGENQQEFKERQQLLSR
                     LCHIRHKIIVLSGKGGVGKSTVAVNLAVALMLAGKRVGLLDVDIHGPSIPTMLGLEDE
                     VIQKDDDGMIPIELGDLKVMSIGFMLRNPDDAIIWRGPLKMGIIKQFLKDVSWGELDF
                     LIIDSPPGTGDEPLSVCQLIGDLDGAIVVTTPQKLAAVDVRKSITFCRHLNVKVLGIV
                     ENMSSFVCPKCGEVTPIFGTGGGKKIAESMQVPFLGSIPIDLKIGQTGDSGQVFIHQY
                     SKSPTAEIMRQIIKPILDMDSENVKS"
     gene            complement(6408..7289)
                     /locus_tag="PKX09_06690"
     CDS             complement(6408..7289)
                     /locus_tag="PKX09_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU17544.1"
                     /translation="MQELVIISGKGGTGKTSIAAALAVLAKSAVVADCDVDAADLHLV
                     LSPQLKERHDFFAGHEAVIRQADCIACGDCLAHCRFEAILTRQEASAGNKFFVDPIAC
                     EGCGVCVQFCPVKAIDFPDRLCGEWFISATRVGPLVHAKLSIAAENSGKLVSTVRQSA
                     RRLAESEKRALVIVDGPPGIGCPVIASITGATQVLVVTEPTVTGEHDLERVLALTRYF
                     NIPTSVCVNKWDINPGMTEQIEKKARKSSAKTVGRIRYDLSVTKAQLQALSVVETDSP
                     CAMDIKNVWNKLGLTGD"
     gene            complement(7301..7621)
                     /locus_tag="PKX09_06695"
     CDS             complement(7301..7621)
                     /locus_tag="PKX09_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOU17545.1"
                     /translation="MKLGMIITQTDAETVFNGLRLALYSLEQGDEVRIFLSGKGVEID
                     QIEDSKFDVKTLAQKVIDKGGQFLACGACLKLRNSDGSEVCPLSTLKDQYQIVRDSDK
                     IVTI"
     gene            complement(7662..8375)
                     /locus_tag="PKX09_06700"
     CDS             complement(7662..8375)
                     /locus_tag="PKX09_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU17546.1"
                     /translation="MQFLVLYDNSSLSPAIRCGWGFACLVDKRILFDTGDEYEPLCEN
                     LAQLKINPKTIEAVVISHDHHDHTGGLWGLLERQPGLKVYACPGFGNDFKQRVNALRG
                     KLVESIGFRKIDDHIGVTGEIPAQYKGSPQPEQALIARTARGIAVVTGCSHPGIITIL
                     TKVKTIFPAEKIALILGGFHLRDSACEAIEEIASQLVTVGVQKVSPAHCTGEPAQAIF
                     KRRFGANYVPAIAGNQIEI"
     gene            complement(8398..9375)
                     /locus_tag="PKX09_06705"
     CDS             complement(8398..9375)
                     /locus_tag="PKX09_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU17547.1"
                     /translation="MNLIDLQYAYGPVPSRRLGKSLGINNIPAKICTYACAYCQVGKT
                     DKMLVERQAFYKPEDIFRNVKYLLDNARHKGEQVDFLTFVPDGEPTLDINLGREIGML
                     KTLGVPVGVITNSSLIWRTDVREELNKADWVSLKIDTVDEAIWRRINRPNRSLDLQKI
                     LDGILQFARHYQGQLVTETMLIRDHNSSQAHIDRLVEFIAQLPDITAYLSIPIRPPSQ
                     KWAQAPTEEILIQAYYIFSSKIRNVKLIIEYEGNDFAFSGNVEADLLNITAVHPMREE
                     AIAKLLEKAKSDWSVVDSLIEKGLLVKKEYWGKTFYTRRIKSLNDEIRK"
     gene            complement(9376..10023)
                     /locus_tag="PKX09_06710"
     CDS             complement(9376..10023)
                     /locus_tag="PKX09_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU17548.1"
                     /translation="MSAFSENRNKQSKLLEKQKSFQFRTAQYLRLGYDRFAASDFVIA
                     AGGDLSSPALDIGTGKGLTAMALAKKGLDVISVDIETDEQAFALFLAREAGLEHRIKF
                     ISQNAAELPYPDNYFRCVVLSNILHHLENAAPVLEEAARVLAPRGIMIIADFSAEGFE
                     IVARLHREDGHLHSVSGETLKTAERFLNTKGFKTTNCLSGHMQEIIVLTQNDGME"
     gene            complement(10029..10865)
                     /locus_tag="PKX09_06715"
     CDS             complement(10029..10865)
                     /locus_tag="PKX09_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU17549.1"
                     /translation="MIIAVASGKGGTGKTTIAVNLAYALDREVQLLDCDVEEPNAQLF
                     LNGALLSTEEVTIPIPQIDASLCNGCGECSRFCEYNAIVSYGTMPLVFADMCHGCGGC
                     MKVCPPQAIREVDHRIGVIETFSAGKITLIQGKLDIGAAMTPPLIRAVKRKMDNRIPI
                     LLDAPPGTSCPVITTLRGADFVALVTEPTPFGLNDLILAVETVREMDLPFGVIINRSG
                     SGDARVNFYCADENIPILAEIPDDRKIAEAYSRGELIGKVFPEYRLIFEKIINDITTR
                     SD"
     gene            complement(10862..11737)
                     /locus_tag="PKX09_06720"
     CDS             complement(10862..11737)
                     /locus_tag="PKX09_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOU17550.1"
                     /translation="MDLDCIPCFVQQAIRAARHLSDDPDVQERIVREALCCIGAVDFR
                     EPPPRASQIFYRRLSEITGIPDLYQADKDRQNRLALKLLPALKEQIGISPDPLLLAAR
                     LAIAGNIIDLGANGNLTVADIHTAVNQALNEQLVGDWEKFKSAVSEARDILYLADNAG
                     EIVFDRLLIEQILPKPMTLVVRGAPTINDALQTDAKAAGLDKLVDIIDNGSDAPGTLL
                     GDCSREFLRCFATADLIIAKGQGNYETLCNEPRNIFFLFKIKCPVIARHIGHPVGTQV
                     LLKSNEIQPENRSIH"
     gene            complement(11743..12225)
                     /gene="tsaA"
                     /locus_tag="PKX09_06725"
     CDS             complement(11743..12225)
                     /gene="tsaA"
                     /locus_tag="PKX09_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOU17551.1"
                     /translation="MRIASVSFRPIGIIHSQHQNAEQIPIQPVYAKDCPAKVEVYQKF
                     AEGLRDLDGFSHIYLIYHFHRAKPAKLRVKPFLQDVERGVFATRSPNRPNAIGLSIVE
                     LIRREGNILHIKGADILDGTPLLDIKPYIKRFDRIDNSRDGWQEEIDEQTAQIRGRRK
                     "
     gene            complement(12222..12584)
                     /locus_tag="PKX09_06730"
     CDS             complement(12222..12584)
                     /locus_tag="PKX09_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOU17552.1"
                     /translation="MKIAFTTSGNDLNAPLDNRFGRAEKFLIYDLENDTFEIIDNRQN
                     LNAAQGAGIQSAETIVRSGAKALVTAHCGPKAFRVLQSAGVKIYTTSAATVASALADY
                     RAGKLSESKSEDVAGHWI"
     gene            complement(12664..13377)
                     /locus_tag="PKX09_06735"
     CDS             complement(12664..13377)
                     /locus_tag="PKX09_06735"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOU17553.1"
                     /translation="MEPINETQSVSMPRIGDPAPAFKAITTQGEINFPQDYRGSWVIL
                     FSHPADFTPVCTSEFMTFATMEKQFADVNCKLVGLSVDGLYSHIAWLRTIKEKIDYKG
                     MKNVEVKFPLIEDITMEVAKKYGMIQPGESNTKAVRAVFVIDPKGIIRTIIYYPLSLG
                     RNFDELLRVVIALQTADAFNVSTPADWRPGDDVIVPTAGSCGVAKERMESKEGIKCLD
                     WFFCLKEITKEDVWKAITK"
     gene            complement(13493..13996)
                     /locus_tag="PKX09_06740"
     CDS             complement(13493..13996)
                     /locus_tag="PKX09_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOU17554.1"
                     /translation="MGTKAIEILNLDVKHLIDLLNRAYADEWLAYYQYWVGAKVVKGP
                     MKGAVIAELEEHAADELRHADMLVKRIIQLGGTPLIKPEDWYKHTNCGYDAPDDPHVK
                     EILEQNIKGEQCAITVYKKLFDLTAGKDPVTYNMVLEILEDEIEHEDDLQAELEDFEM
                     LVEKHSK"
     gene            complement(14281..15129)
                     /locus_tag="PKX09_06745"
     CDS             complement(14281..15129)
                     /locus_tag="PKX09_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOU17555.1"
                     /translation="MKLNLKSKIPTIICKWIIVFLFGTGSLLSAQLDTLVIPSVAMSK
                     NPKAVVVLPDVYPKSNDRFPVVYLLHGWSGSYRDWPTKMDLKPLADKYGFIIVCPDGG
                     YAGWYVNSPIVKDSQYETYIAKEVVDFIDANFRCVADSTGRFLCGLSMGGHGAFTLLA
                     KFPERFAGAGCMSGAMTMSSGERRAGLADVLGDWETNRQRWEANSALFLAERLVGHDK
                     AILIDCGVDDFLHQTNFLLHKKLLDLKIPHVYIERPGAHTWDYWTNALEYHLLFFKKW
                     AEKSQG"
     gene            complement(15431..18181)
                     /gene="ppdK"
                     /locus_tag="PKX09_06750"
     CDS             complement(15431..18181)
                     /gene="ppdK"
                     /locus_tag="PKX09_06750"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOU17556.1"
                     /translation="MTTQKSKTPKYVYFFGGGKADGNESMKELLGGKGANLAEMAGHP
                     DLRLPVPPGFTITTEVCTYYYQHNRKYPAELKEQVEAALTKVEKIMGRKFGDPDNPLL
                     VSVRSGARKSMPGMMETVLNIGLTEKTIPGLIKQSGGNERFVYDAYRRLIMMYSDVVM
                     EKAAGIEPKDDEGIRRQLEKIMDDFKKAKNYTSDTDLTADDLKELCRVFKEQVKKVLG
                     KDFPDDPYEQLWGGIGAVFASWNGKRAVAYRRIEGIPDDWGTAVTVQAMVFGNMGDDC
                     ATGVAFSRNPGNGENQFYGEYLINAQGEDVVAGIRTPAPINKYSASEHNKDLVTLEEY
                     MPAIYKELVEYRNRLEKHYRDMQDIEFTIEKGRLFMLQCRIGKRNGPAAVRMALDMLN
                     EKLITREEAVLRVSPSQLDELLHPIIDPKHEKKIEPLAKGLPAGPGAATGQIVFSSAD
                     AVEWAKAGKKVILVREETNPEDVDGMRASQAILTARGGMTSHAALVARGWGKCCIVGC
                     SAIHIDYQKKVMKIGDRVFKEGDFITLNGTKGYVYAGTLPMIDATENPDLVKFLKVCD
                     SVRRLAIRTNADTPADAKRARQFGAEGIGLFRTEHMFYGEGSDAPLFALRKMIVSNTF
                     DERQKALDELFPYVRKDIKATLEAMDGYPVVIRLLDPPLHEFVPREKEKQEKLARDLN
                     ISLDELEKRAAELHESNPMMGHRGVRLGVTYPEISRMQIRAILEASAELLKEGKKPYP
                     EIMIPVVSLTKELEHQKAIVDEVYQEVLKKFGLKKIKYLFGTMIEIPRACLVANEIAG
                     VTEFFSYGTNDLTQMGFGFSRDDIGSFVPDYIEKKILPEDPFQTIDQVGIGELIQIGI
                     ERGRKTKPNLEIGICGEHGGEPRSVKFCHRVGMDYVSCSPFRVPIARLAAAQAVAEEK
                     TK"
     gene            complement(18423..19904)
                     /locus_tag="PKX09_06755"
     CDS             complement(18423..19904)
                     /locus_tag="PKX09_06755"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOU17557.1"
                     /translation="MGKQVSNEQMDKLVSLCKRRGIIFQSSDIYGGLSSTWDYGPLGV
                     ELKRNVKDLWWHDMVTSRENVVGMDAAILMHPKVWEASGHVANFHDPLIDCKKCQARF
                     REDKAPAYFKCSNCNFNFYVNDGLIEVKKEFAAHFPDLYAREIAHYRDSDCPHCGGQR
                     TIKLEKIACPNCGSSELSPARQFNLMFKTHFGPSEDSASEVYLRPETAQGIFVNFDNV
                     ISTTRVKLPFGVGQIGKAFRNEITTGNFIFRSREFEQMEMEFFVKPEESGRWYEYWVN
                     ERFNWYKKLGVRAENLRRRPHEKEELAHYAKACTDVEYKFPFGWSELEGIADRQTFDL
                     EQHMKASGKKLTYFDSETNTHIVPAVVESSAGVDRTILTVLCDAYWEDAEHDRVVLRL
                     DPKIAPTKVAILPLVNRDGMPEIAEAIFNDVKKQFAAFYDQAGSIGRRYRRQDEAGTP
                     FGITVDSQTLQDGSVTLRDRDTLEQIRLPKENIVKVLQEKLAL"
     gene            complement(19986..22193)
                     /gene="topA"
                     /locus_tag="PKX09_06760"
     CDS             complement(19986..22193)
                     /gene="topA"
                     /locus_tag="PKX09_06760"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOU17558.1"
                     /translation="MELLIVESPSKAKTIKKYLGNNFKVMASVGHIKDLPEKELGIDI
                     QHDFKPKYVTIKGKSKIISNLKESAQSAQRVYLAPDPDREGEAIAWHIANVIDVPSDR
                     VQRVLFNEITESGIRYGLSHPRQIDMSLVNAQQARRVLDRLVGYQVSPFLWRTLYSGL
                     SAGRVQSVAVRIICEREVEIEQFIPLEYWEITALLQTPQNEKFSAKLIKIDNKKTAIS
                     NASDANKHLAILKNATYQVSNIEEKEVTKSPSPPFTTSTLQQEATRRFRYSPEKTMRI
                     AQNLYEGVEIGGEPVGLITYMRTDSVRISKEAITGIREYIEKHYGAKFLPPNPRHFKT
                     TKRNVQDAHEGIRPTHFEYTPEVVAPYLSPEQKKLYTLIWNRFAACQMSAAVYKQKII
                     DVTADNYLFRATGSDLIFDGYLRAWREEKAEDEKKIALPINIQKDDRLTLLELQPEQK
                     FTEPPPRFTEGTLIKELDNLGIGRPSTYATIVSTIITRKYVERKGGYLVPTDLGKTVN
                     KLLVDGMPDIFNVKFTARMEAELDDIEGNRKEWQKVISEFYQPFHKSLEELQAQRREI
                     KTGLQEKTNEKCEICGAPMVIKWSRNGRFLACSAFPECKNTRPLNTAPVAPTESKTCP
                     NCGSPMTIKDGRFGKFWACTKYPKCKTTEPFTLNIKCPEPDCDGQIVQKRTKKGKIFY
                     GCNRYPNCKFATWNEPVARPCQNCDSQFLERKIKRDGSVLLICPKCKTETADE"
     gene            22749..22822
                     /locus_tag="PKX09_06765"
     tRNA            22749..22822
                     /locus_tag="PKX09_06765"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22783..22785,aa:His,seq:gtg)
     gene            22836..22910
                     /locus_tag="PKX09_06770"
     tRNA            22836..22910
                     /locus_tag="PKX09_06770"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22869..22871,aa:Glu,seq:ttc)
     assembly_gap    23134..23233
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23445..24050
                     /locus_tag="PKX09_06775"
     CDS             23445..24050
                     /locus_tag="PKX09_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17559.1"
                     /translation="MRLTKNQQNALRVARLISVILHPLVIAPLTYALIIAHTSEINRT
                     QKLLYLGLVLIAIIFIPLISVLYLKKRGETTSLDVPERDKRISPFLVCIAGYFIVWIL
                     LKVSGAPRPISLLMWCYGFNSIIATIITKYWKISVHGMALGGPIAALGFVISPTFYWG
                     ILIAPLIVYSRVKLKAHTIAQVITGFLLGFLLTLIQFEIFL"
     gene            24047..>25091
                     /gene="glgC"
                     /locus_tag="PKX09_06780"
     CDS             24047..>25091
                     /gene="glgC"
                     /locus_tag="PKX09_06780"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOU17560.1"
                     /translation="MKIIDVLAIILGGGKGTRLYPLTKYRAKPAVPFGGKFRIIDVPI
                     SNCLNSGINKIYILTQFNTHSLHRHIYSTYSLGPFSNGFVDILAAQQTMENTDWYQGT
                     ADAVRKNLPFINEIKAAYTMILSGDHLYRMDYRLFLEHHIKNKAEITVAVKPVSRPDA
                     GHFGILQTDEKCRIVQFVEKPNSELLDKLKSPGLPDETPFLASMGIYIFATETLHKIL
                     GQNPSDDFGKNIIPASIPTLNVCAYQFDGYWRDIGTIESFFTANLELARFQPPFSLYD
                     KHAPIYTHARSLPASRLENCSLTQTLLADGCHIGESNISESVVGIRTIIQKGTTIEKC
                     VIMGADYFEQVQPE"
BASE COUNT         6746 a         5813 c         5345 g         7087 t
ORIGIN      
        1 gaaaagactt gatgggtgtt ttcataccct ggtttatcat ccaaccattt tatattgttt
       61 taatgggtat tttaagcttt ggaggaaatc ggctggactg gggacacagg agttcgtctc
      121 acgacgcggt gaagtaacat taaaaccagc ccgatgaatg ctggcattaa agaatttgtt
      181 acgaaaacaa acaggcttgt agcaactgct accggcgcgg ttataccaaa caatccgaat
      241 aaatatacag ctacgttttc gcggacaccc agccccccaa atgtaaaagg cacactattg
      301 gcaaacagta taaaaatcac gctctgagcc gcggctgacc aggaaattga accagcccaa
      361 ttaataataa aatgatattc actgctgatt aaaatcaggg agaaaacagt caggataccc
      421 gatttaagta ttaatgacca tcggatggta ggattgggaa gatatttctt tacgaatctg
      481 atttttttaa gatacggaaa gccagcacag gcaccaatcg tgacaattcc gatactgata
      541 aaaatccatt tatgatgcgg aaaaagatgc aagccagccc aaaccaacaa tataatttgg
      601 gtcagcatta cacttgacaa ttcgacgaga taagccataa atccatggcg gtgtttgccg
      661 ttcaagtaat agattttcaa aaattcggca tatcctcctg ggattaccaa ccgaaaagta
      721 tgaccgatga aaaaggattt aatagtttca tgccatttgg ctgactcgga atactctttt
      781 aaaatgatac cccagcgaat tatctgaaac gcccagttaa acataatgag aacacataag
      841 aaaaggattc ttgaccagga aatagtactg aaaatccacc ttaggtcagc cagattgatt
      901 ttgcggatgg cgatatttac taagacgata ctgagcgcca gttttataac gagtaatatc
      961 gattctttaa aatagaatga cgcagattta cggggcaaag cagtttattt ccagcgatag
     1021 attttcaatg agccgatcac ctgcgataac aaatagcaga ccgggtaaat tacccataga
     1081 atcggaaaat atttgagcaa atccgtttgg tgaaataagt gggcgccgtg ataaacggcg
     1141 ataaattcag ccataaattt gaaaaacaac atcattccca accaattcca aagtgacggc
     1201 gaaaaaagca ttattgccag cacaacgtga aataggaaaa caatgacccc cagaataaaa
     1261 aagggaatag tcaggttaaa tgctttacta ttccggcgca actgttgatg gatatatttt
     1321 ttaccgaact tggttggctc tgtggtaaca tagctggctg ggtcaatgtt ataagcaaac
     1381 cgccagcgag ttctcgtgcg gatttcctgc attagaaaaa aatcatcgcc tgtatcggta
     1441 ttagcgcttc gataatagcc attgacatct tcaaaagcct ttttccggta agccagatta
     1501 cgtccgttgg catgcgtcgg tttattataa aaagtcgaag cggcggcaac ggacgcttca
     1561 caaagggtat cgaaccgttc ataaatgttc ataaaccctt tgcgtcttgc gaccgggcta
     1621 tgaccgagaa ccatgccaac ttctttgcgg aaatatgata ccatcgaagc aatccaattc
     1681 ggcggaacta cgcaatcagc atcggttact aaaataattt cgccacaact attctgaaca
     1741 cccagcgtga tagcgccttt tttacctttg agttgatcgg tagttttatc atgatggaga
     1801 accttccaat tggattttcc ggaagcaaaa tcgttcataa tgcttagagt atcatcagta
     1861 gaatcatcat caaccagaat tatttcatat ttgtcttcgg gatagcgcaa attggtcagg
     1921 ctttgcaata accggctaat aatttgttct tcattgcggg cgcagacaat gactgaaacc
     1981 gcaagtgtct gaaacactcg tttctgggtg cgaataaata tcaagccaat tccgactact
     2041 cccattataa ttagataaaa aaatgtcagc aggatcgcaa attcaattag attaagcatc
     2101 agaaattgat ttcagtttca ataaatccaa agcggtagga ttcttggtcg ttattaagtt
     2161 tagtattata caaattaatt ccggctttaa tgaaaaagtc gttaaatatc tcatagcggc
     2221 cggcgacctt gagctgattg gttgtctcaa tatcacccat cagccacttg gtggcgtgat
     2281 catattgctc gtcacgtagc tcataactga cagtggcgtc tccgccccaa aaatgagtgg
     2341 aatcgtcaac cccctttttc aggatcaggt attcgctggt caaggttaag cgccttgaaa
     2401 gcgctgtccc aattttaaaa taccaggttt gggaattggc gccgtatgga aatccaaggc
     2461 aaatgctatt atgagtataa ttggtagtca gagtcttatg ggaataagtc caggggcgca
     2521 cggcggtaaa ttctgcctga aaatcaaaag gcaaggtggc aatgttaggc gaccagcgca
     2581 tgcccgcctg caaagcgtgc ttattagccc accattgttt accaagttca ctaaaacgca
     2641 tttcgtcaat tagaaccgag ccatagaacg taatattctt gataaaggtc gttttaaaat
     2701 ccaccgccat tagagtattg tcgcggtctt caagagcgtg tccgatcggc caataaaatc
     2761 caaagggtgt catataggct atatcaattt ctcgatcacc ataaatcacc atctcgctaa
     2821 acccgacagt agtcgttccg cggaaaaaag gaatgtcgag acgatgggcg gcgatatact
     2881 tggaacgatt tctctgctgc aatgcaatat cgcgataatg ggtgctgtcg ttcaatagcg
     2941 atccatgaat catagacagc tttatccctt tgaatttggt atcgaatcca atataggaaa
     3001 acagcggcgc actttttgat aaaatcaagt tgttagtccc acttgctccc caggcaaccg
     3061 gctgacggta taagccgata ttgaagtatt tttgatgaaa aacaagagaa gtctgcaaaa
     3121 tatcaaaggt gaccgtcccg ccgggttgta ccatactgaa gttgccgatt tcctcctcat
     3181 agggttccgt aaacgccgga tcaccatttt tcataaagcg ggcaaagtag gtgtgaaaag
     3241 tgagcgattt actcagcgcg cctcgtaata gatagcgatc ggaaaacatt tgcttctggt
     3301 gattttcttt ccaatccatt cgaccacgca ccccaatatc aaaccagaaa aaggtctcgc
     3361 cactttcata catcaaaaga tgattttcct cgaccgcatc ttgtcgcgaa aacagattgg
     3421 ccgttgcctg ccgaaaatag ttattttgat aaaatggggc tgagatcgaa tccggttcgg
     3481 tcaattggcg ttcagtttca tagcgataat cggcgaggaa ttcacgtaaa atattttttt
     3541 cgatgaccga aagtttatct gccttttggg aggcgatttg caggaaatta acgatttcat
     3601 cacgggttaa cggtaaagtc agatcgttaa atcccttgat gatgccccgc gtttcgaggc
     3661 gtttcaaaaa atcatagacc ggatcggtag ctggaacgtc aataccgata gcagaaatta
     3721 ccagcggaaa agcggtaatg ataataaaag ttagatttac aaggcgaata tttctcatca
     3781 tttgcgctga aatatacaat tattttatca gtagcgaaaa tgcttaatgg gttcgcccat
     3841 tcttccaata ttagtcatag cgtctatacc gatttgtaag tgaatctgac tccattgcgc
     3901 cgtaacttgc ttatctgact cttcagtttt aataccttcg ggagtcgttg gtaggtctga
     3961 aacgaggagt aaagcgccgc gcgcgatctc gttataatgt ccgacgataa aaagggtggc
     4021 ggtttccata tcaatagcga tacaagtcat ttcgcgtaag cgttggtgga acgcctgatc
     4081 atgttcccaa actcgccggt tggttgtata aactacaccg gtgcggtact caagaccacg
     4141 ctggactata atttctgaga caaatttatg caatttgaac gatggcaaag ccggaacttg
     4201 gggtggaaaa taatcgtcgc tggtaccctc gccgcgaata gcagcgattg gcaaaataaa
     4261 atgcccaatt tcagttgatt tcttcaaccc gccacattta cccagaaaaa gcactccctt
     4321 cggatgaacc gccaccagca aatccataat agtggcggca ttcggcgaac cgattccgga
     4381 attgataata gtcaatccgg ccgaatttgt ggcagcttgc atcggacgat tttcaccgta
     4441 caaatcacaa ctaaacatcg tacaaaaaat gtccacataa ttgcggaaat tagtcagcag
     4501 aatgtaatcg ccgtattttt cgaggggcag accagtataa cgtggcaacc agctccgagc
     4561 aatttcgatt ttttccttca taactacacc ttcttcaaat tgaaaataaa atcagagcca
     4621 ttaaagcaca gaacacacct aaaccctgtc tgaaactaaa cggttctttt aaaaagatta
     4681 acgccagaac aatactaatc aatgggtaga gagccgtcat cgtcacaatg gtagtaacct
     4741 tgccacgact gacagcctgg agaaaaaaca gcgaacccag cataccaaac agaccggtta
     4801 aaattgccca gaacgcgcca actgcctgcc attgaatttg gctcctaacc gatagtaaaa
     4861 cccccaaacc aacgattgtc ccaccaatgg cttgatagac gagtgcgctc gacggatcaa
     4921 tgtagcgagt ggctaccttc ggtaaaaagc cccaaaatcc ccataagatt aaagcaatta
     4981 gtgcaggtaa agtccaactc ctcaagtcaa ttctccttgt gaataattcg attaaactat
     5041 caaatttgct gagcaaagta caaaaattct tctggccagt caaatttatc taatctaatt
     5101 atagatatga agatatatgt ggatagtgat taaaaacatc aatgatcaca gacattgctg
     5161 gtcagttgca gatttccatc gaaataatca ccgattaact tttccggtga ttcagaacag
     5221 gcgcccacga gcacctgaat attattttcg gcgaaaagca tcagcgcgcg ctgtcccata
     5281 ccgccagcga taattagatt tacgccgtgc tccgccagcc agctaggtag taaacccggc
     5341 tcgtgcggcg gcggtataag atcggtgcgt ttgattattt tcttattctg aacttcaaca
     5401 tcaaccaacg caaattgttc gcaatgtccg aagtgcaggg ttagtttgcc gttggcgaga
     5461 ggaatggcta ttttcatttt tttattctcc tgtgattaag acttaacatt ttcggaatcc
     5521 atatccagaa ttggcttgat gatttgccgc atgatttccg cagttgggct tttactatat
     5581 tggtgaataa agacctgacc gctgtcgccg gtttggccaa ttttcagatc aatcggaata
     5641 gaacccagaa atggtacctg catactttcg gcaatcttct ttccgccacc ggtaccgaaa
     5701 atcggcgtga cttcgccgca tttcggacaa acgaaactgc tcatattttc cacaataccg
     5761 agtactttga cattcaaatg acgacaaaat gtaattgatt tgcgcacatc cactgcggcg
     5821 agtttttgcg gagtagtgac gacaattgcg ccatccaggt cgccgattag ttgacaaacc
     5881 gaaagcggtt cgtcgccggt tccgggcggc gagtcaatga tcagaaaatc cagctcgccc
     5941 cagctgacat cctttaaaaa ttgcttaatg atacccattt tcaaaggccc gcgccagata
     6001 atggcatcat ccggatttct gagcatgaag ccaatcgaca taactttcag gtcgcccagc
     6061 tcaatcggta tcatcccgtc atcatctttc tgaattactt catcttccag ccccagcata
     6121 gtcggaatgc ttggtccgtg aatatccaca tccagcaaac cgactctctt acctgccagc
     6181 atcagagcca cggccagatt aactgccacc gtgcttttac cgacgccacc tttgccggac
     6241 aacacgataa tcttgtgccg gatgtgacaa agccgggaaa gtaattgctg gcgctctttg
     6301 aattcctgct gattttcacc ttcccgaggc tttgccgccg aacaggtcga attaccacag
     6361 gaatcgcagg tactttttgt tttttcttta ctaatatcac tcatatttca atctcctgta
     6421 agacctaatt tattccagac attctttata tccattgcgc agggcgaatc ggtttctacg
     6481 actgataagg cttgtaattg cgcttttgtc acactcaagt cataacgtat ccgaccgaca
     6541 gttttggcgg aactctttcg ggcttttttt tcaatttgct cagtcattcc aggattaata
     6601 tcccacttgt tgacacatac tgaagttgga atattgaagt agcgcgtcag agccagcacg
     6661 cgttccagat cgtgttcgcc ggtaactgtc ggttctgtca ccacgagcac ctgggtcgcg
     6721 ccagtaatcg aagcgatgac cggacaaccg attcctggtg gtccgtcaac aataactaaa
     6781 gcgcgctttt cagattcggc aagtcggcgg gcagattggc gtaccgttga gaccaatttg
     6841 ccggagtttt cagccgcgat actgagcttg gcgtgcacta acggaccaac gcgcgtcgcc
     6901 gaaataaacc attcgccgca gagacgatcc ggaaagtcga tcgccttcac cgggcaaaat
     6961 tgcacacaga cgccgcaacc ttcgcaggcg atcggatcga cgaagaattt atttccggct
     7021 gacgcttctt ggcgggtcag gattgcttca aagcgacaat gcgccaggca gtcgccacag
     7081 gcaatacaat cggcttgacg gataacggct tcatgtccgg caaaaaaatc atggcgttct
     7141 ttcaactgcg gcgaaagaac caggtgtaag tcggcggcgt ccacatcgca atcggctact
     7201 accgcacttt tggctaaaac cgccagtgcc gccgcgatgc tggttttacc tgtaccgcct
     7261 tttccactga tgataactaa ttcctgcaat gccggattcc tcagatagta acaattttat
     7321 ccgaatcgcg gacgatctga tattgatctt ttagagtgga aagcggacag acttcggaac
     7381 catcggagtt gcgtagtttt agacaggctc cgcaagccag gaactgaccg cccttgtcaa
     7441 ttaccttttg agccagcgtt ttgacatcga attttgaatc ttcaatttga tcgatttcga
     7501 cgcctttacc ggaaaggaaa atgcgcactt cgtcaccctg ttcaaggcta tagagtgcca
     7561 gacgcaatcc gttaaaaacg gtctcggcgt cagtctgagt aataatcatt ccgagtttca
     7621 ttttgcattt cagcctttca tttacacttt tttgctctca tttatatttc tatttgattg
     7681 ccggcgatgg ctggaacata attcgcaccg aatcttcttt taaaaattgc ctgggcaggc
     7741 tcgccggtac aatgggctgg actaactttc tgcactccta ctgtgaccaa ttgagaagca
     7801 atttcttcaa tcgcctcgca tgcgctatct cgcaaatgaa atccgcctag gattaatgca
     7861 attttttcag cgggaaaaat tgtcttcact ttcgtaagaa ttgtaatgat tcccggatga
     7921 gaacagccgg ttaccactgc gatcccgcgc gccgttcggg caatcaaggc ttgctccggc
     7981 tgcggtgaac ccttatattg ggcgggaatt tcacccgtta caccaatgtg atcgtcaatt
     8041 ttcctgaaac cgatcgattc gaccagctta ccccgtaaag cgtttaccct ttgcttaaaa
     8101 tcattgccaa atccgggaca ggcatatact tttaagcccg gctgtcgttc taataatccc
     8161 cataatccgc cggtatgatc gtggtggtca tgtgaaatca cgactgcttc tattgtctta
     8221 ggatttattt ttaactgtgc caaattttca caaagcggct cgtactcatc gccggtatcg
     8281 aagagaatac gtttatctac cagacaggca aatccccagc cacagcggat tgccgggctc
     8341 aatgatgaat tatcataaag cactaagaat tgcattttcc gtctccggca accttgttca
     8401 cttgcggatc tcatcgttta aagattttat gcgtcgggta taaaatgttt tcccccaata
     8461 ttcttttttt accaaaagcc ctttttcaat taaactatca acgaccgacc agtcagattt
     8521 agctttttcc aataatttgg caatagcctc ttcgcgcatc ggatggacag cggtaatatt
     8581 caacaaatcc gcttccacat tgccgctaaa agcaaaatcg tttccttcat attcaattat
     8641 taatttcaca ttccggattt tagagctgaa aatataataa gcctgaatca gtatttcttc
     8701 ggttggagct tgggcccatt tttgggaagg cgggcgaata ggaatggaaa gataggcggt
     8761 gatatccggc aactgggcga taaattccac cagccggtcg atatgcgcct gactgctgtt
     8821 atgatcgcgg atcagcatcg tttcggtaac aagttgacct tgataatgcc tggcgaattg
     8881 cagtatgcca tcgagaattt tctgaagatc aagcgaccgg ttcggacgat taatcctgcg
     8941 ccagatcgct tcatcaaccg tatcaatttt cagcgaaacc cagtcagctt tattcagttc
     9001 ttctcttacg tctgtacgcc agatcagcga actgttggta atcacaccga ccggaactcc
     9061 caaggttttc aacataccga tctcccgacc aaggttaatg tccagcgtag gttcgccatc
     9121 cggaacaaaa gtcaggaaat caacctgttc gcctttgtgc cgggcattgt ccaaaagata
     9181 tttcacgttc ctgaaaatgt cttccggttt gtaaaaagcc tgacgttcaa ccagcatttt
     9241 gtcggttttt ccgacctgac agtaagcgca ggcataagtg catattttgg cggggatatt
     9301 gttgattccg aggcttttcc ccagccgtcg cgatgggacg ggaccgtagg catattgtaa
     9361 atcgataagg ttcaattatt ccataccatc attttgagtc aaaacgataa tttcttgcat
     9421 gtgaccgctt aggcagttag ttgttttaaa tccttttgta tttaagaatc tttccgccgt
     9481 tttcagtgtc tctccgctga ccgaatgcag atgaccatct tcgcgatgta agcgcgcaac
     9541 gatttcaaaa ccttctgcag aaaaatccgc tatgatcata atccctcgcg gcgccagaac
     9601 tcgggcggct tcttccaata ccggcgcggc attctccaga tgatgcagaa tatttgaaag
     9661 aacaacacat ctaaaataat tatcgggata aggcaattcg gcggcatttt ggctgataaa
     9721 tttgattcgg tgctcaaggc cggcttctct ggctaaaaac aacgcaaagg cttgttcatc
     9781 ggtctcaata tcaactgaaa ttacatccaa tccttttttc gccagcgcca tagccgtcaa
     9841 ccccttgccg gtaccgatat ccagcgccgg actggaaaga tcgccacccg cggcgatcac
     9901 gaaatcggaa gccgcaaagc gatcatagcc caaacgcagg tattgggcgg tgcggaactg
     9961 aaaagatttc tgtttttcaa gcaatttgct ctgtttattt ctattttctg aaaaagcgct
    10021 catatatttt aatcacttcg ggttgtaatg tcatttataa ttttttcaaa tattaggcga
    10081 tattcgggaa aaacctttcc tatcagttcg ccgcgcgaat aggcttcggc aatctttcga
    10141 tcatccggaa tctccgccag aatcgggata ttttcatcgg cgcagtaaaa atttacccgc
    10201 gcatcgccac ttccggaacg gttaataatg accccgaagg gtagatccat ttcgcggacg
    10261 gtttcaacgg ccagaatcag atcatttaat ccaaaaggtg tcggctcggt cacaagtgca
    10321 acaaaatcgg ctccccggag cgtcgtaatt accgggcagg aagttcccgg cggtgcatcc
    10381 aataatatcg gaatacgatt gtccattttt cgtttcacag cccgaattaa cggcggagtc
    10441 attgccgcgc caatatccag tttaccctgg atcagagtaa ttttcccggc actaaatgtc
    10501 tcaatgacgc caatacgatg gtcgacttca cggatcgctt ggggcggaca gactttcata
    10561 cacccgccac atccatgaca catatctgca aatacgagcg gcattgtacc ataggataca
    10621 atggcattat attcgcaaaa acgactacat tcgccgcaac cattacaaag acttgcatcg
    10681 atctgaggaa tcggaattgt cacttcttca gtagagagca gagcaccatt cagaaacaat
    10741 tgggcattcg gttcctcgac gtcacaatcc agaagctgta cttcacggtc cagggcatag
    10801 gccaggttga cggcaatggt cgtcttgccg gttccaccct taccggacgc aaccgcgatg
    10861 atcaatgaat gctcctgttt tctggctgaa tttcattgga ttttagcaaa acctgggttc
    10921 cgaccggatg accaatatgc ctggcgatta ccggacattt tattttgaac aggaaaaaaa
    10981 tattgcgcgg ttcgttgcaa agagtctcat aattgccttg acccttggca atgatcagat
    11041 cggcagtcgc aaagcacctt agaaattcac gactgcaatc accaagcaga gttccaggcg
    11101 catctgagcc gttatcaata atatcgacaa gtttatctag accagcggct tttgcgtcag
    11161 tttgcaaagc atcgttgata gttggagcgc cacgcactac cagtgtcatc ggtttaggta
    11221 aaatctgctc gatcagcaga cgatcaaaaa ctatttcacc ggcattgtcg gccaagtata
    11281 gaatatcgcg ggcttcgcta accgccgatt taaatttttc ccaatcgccg acgagctgtt
    11341 cattcagagc ctgattgacc gcggtatgaa tgtccgcgac cgtcagattc ccattggcgc
    11401 cgagatctat aatatttccg gcaattgcca ggcgcgccgc cagcaaaagc ggatcgggcg
    11461 aaatgccgat ctgttccttt aacgctggca gtaatttgag tgcaagccgg ttctgtctat
    11521 ccttatcagc ctggtataaa tccggaatgc cggtaatttc actcaggcga cggtaaaata
    11581 tttgactggc gcgcggtggc ggctcgcgaa aatcgacggc accgatacag cagagagctt
    11641 ctcttacaat tcgttcttga acatctggat catcagataa atgccgcgcg gcccggatag
    11701 cctgctgtac aaaacaagga atacagtcga gatccatttg cattatttcc ggcgccctct
    11761 aatctgagcg gtttgttcat cgatctcttc ctgccagccg tcacgactat tatcgatccg
    11821 gtcaaagcgc tttatatacg gtttaatgtc gagcaaagga gtaccgtcca aaatatctgc
    11881 tcctttgata tgcaaaatat tgccctcgcg tctgatcagc tcgacaatgc tcaatccgat
    11941 cgcgttaggt cggttgggag accgtgtcgc aaatacgccg cgctcgacgt cttgcagaaa
    12001 aggcttcacc cgcagtttag ccggtttagc ccgatgaaaa tgatatatca ggtaaatatg
    12061 cgagaagcca tccagatcac gcagtccttc ggcaaatttc tgatagactt caacttttgc
    12121 cgggcaatct ttggcgtaaa ccggctgaat tggtatctgt tccgcgtttt gatgctggct
    12181 gtgaattatc ccgatcggtc ggaatgaaac tgaggcaatt ctcatatcca atgtccggcg
    12241 acatcttcgc tcttagattc gctaagcttg cctgcccggt aatccgccag ggcggacgcg
    12301 accgttgccg cacttgtggt ataaatcttg acaccggcgg attgcaaaac ccgaaaagcc
    12361 tttggcccac aatgggcggt gaccagcgct ttggcgccgc ttcggactat ggtttccgct
    12421 gattgaatcc cagcaccctg agcggcattc aggttttgcc ggttatcgat tatctcgaat
    12481 gtatcatttt caaggtcata aatcaggaac ttctctgctc gcccgaaacg attatcgagt
    12541 ggtgcattta ggtcattgcc tgaagtcgta aatgcaattt tcataataca caccccttaa
    12601 ttcaaggttg cgtgagcctg ataacccacg caacctcgca gttttaattt ccggaatcgc
    12661 caattacttg gtaatggctt tccagacgtc ttccttggtg atctccttca gacagaagaa
    12721 ccaatcgaga cacttaatgc cctctttgct ctccatacgt tctttggcaa cgccgcagga
    12781 gccagcggtc ggaacgatga catcatcacc aggacgccaa tcagccggag ttgaaacgtt
    12841 gaaagcatca gctgtctgca gagcaattac tacccgcagc aattcgtcga aattacgtcc
    12901 cagacttaac gggtaataaa tgattgtgcg gataatgcct ttaggatcga ttacgaagac
    12961 ggcgcgcacg gctttagtat tgctttcacc cggttgaatc atgccgtatt tcttggcaac
    13021 ttccatcgtt atgtcttcga tcaatggaaa tttgacctcg acatttttca tacctttgta
    13081 gtcaattttt tccttaatgg tgcggagcca ggcgatgtgg ctgtatagac cgtcaaccga
    13141 aagtccaacg agtttgcagt tcacatcggc gaattgcttt tccatagtgg caaaggtcat
    13201 aaattccgaa gtgcagaccg gcgtgaaatc agccgggtgg ctaaataata ttacccagct
    13261 tcccctataa tcctgcggga aattaatttc accttgagta gttatagctt tgaaagccgg
    13321 agccggatcg ccgatacggg gcattgatac gctctgagtc tcattaatag gttccatgtt
    13381 tttttccttt tatggtttgt ttatatcgct ttttaaataa gtcggtctga atacactaaa
    13441 aaacataccg gatcaacata tttgctaaac aaacggtatg tttctaactg gcttatttag
    13501 aatgcttttc tactaacatt tcaaaatctt cgagttcggc ttggagatcg tcttcatgtt
    13561 cgatctcatc ttccagaatc tccagaacca tattataggt gaccggatct ttaccagcgg
    13621 taaggtcgaa taatttttta taaaccgtta tagcgcactg ttcgcccttg atattctgtt
    13681 caaggatttc tttaacatgc ggatcatccg gtgcatcgta accacaattg gtgtgtttgt
    13741 accaatcctc gggtttgatc aatggagtcc cacccagctg gattatacgt ttcacgagca
    13801 tgtcggcatg gcgcaattca tcagccgcgt gctcttccag ctcggcgata acggcaccct
    13861 tcatcggtcc tttgacaact ttcgcgccga cccagtactg ataataagcc aaccattcat
    13921 cggcatatgc ccgattcagt aggtcaataa gatgcttcac atccaaatta agaatttcaa
    13981 ttgctttggt acccataatt ttctcctttc attttttcta tttgagacac ggaaaataca
    14041 ttggttgcat tcgaaggtta acatttaggc caatggttct tagctaagga accactccat
    14101 tctctttttc ggtggcataa ccacgatcag ttcaaccggt aacgccatat tttgagatgc
    14161 gtaaaatact aattctttca ctttacctca gaattatttt acacacgctc tttttaatcg
    14221 ctgagtaaaa ccgtcgcttc ctatactact tttctcttaa tcttaatctt agtctcaacc
    14281 tcagccctga cttttttccg cccatttttt aaagaatagc agatgatatt cgagagcatt
    14341 tgtccagtaa tcccaggtat gcgcgccagg gcgttcgata tatacatgtg gaatcttcag
    14401 gtctagtaat tttttatgta gaagaaaatt agtttgatgc aaaaaatcat caactccgca
    14461 atcaattaaa attgccttat cgtgtccgac caagcgctcg gctaaaaata aagccgaatt
    14521 cgcttcccac ctttgccgat tcgtttccca atctccgaga acatcggcca gcccagcccg
    14581 gcgctcgccg cttgacatcg tcattgcgcc gctcatacag cctgccccgg caaaacgttc
    14641 cggaaattta gctagtaagg tgaaagcacc gtgtcctccc atactcagtc cacaaagaaa
    14701 acgcccggtg gaatcagcta cgcatcggaa atttgcatca ataaaatcaa ctacctcttt
    14761 agcaatgtaa gtctcatact gtgagtcttt cacaatcggg ctattcacat accaaccggc
    14821 atagccgcca tccgggcaga caataatgaa accgtacttg tcggctaacg gcttcaaatc
    14881 cattttagtc ggccaatcgc gatatgagcc actccagcca tgtaacaaat atacaactgg
    14941 aaaacgatca ttcgatttgg gatatacatc tggcaaaacg actacggctt tagggttctt
    15001 agacatagcc acgctcggta ttaccaaagt atccagttgg gcagataaaa ggctaccagt
    15061 cccgaatagg aagacaatta tccatttgca gataatcgtt ggtattttgc tctttaagtt
    15121 aagtttcatc attgcctctc tatttgatta tcaaatatct ttattatttc ggaagtctcc
    15181 aatagttttc atcgaatttc aggattgagc tcacgaccaa ttggctgact ttctttgttt
    15241 aaaataactc cggatggtcg gcggtgaaag atgtattggc caaaataata aaaattattt
    15301 ccctcttgat tacgatttcg tcgcattcag caatgagaaa acgccaaaaa taagaagaac
    15361 ggctctactt aaaaaaagaa ccgcagacca aaatctgcgg ctcttcattt atctatattg
    15421 aaaaaacgtt ttacttcgtt ttttcttcgg caacggcttg ggcggctgcc agtcgagcaa
    15481 tcggcactct gaacggtgag caggaaacat aatccattcc aactcgatga caaaacttga
    15541 cggatctcgg ttcgccgcca tgttcaccac aaataccgat ttccaggttc ggcttggtct
    15601 tgcgaccacg ttcgatacca atttggatta attcgccaat gccgacctga tctatggtct
    15661 gaaaaggatc ttccggtagg attttcttct cgatataatc cggcacaaaa ctgccaatat
    15721 catcacgcga aaatccaaat cccatctgag taagatcgtt agtcccataa gagaagaatt
    15781 cggtaactcc tgcgatttca ttggcaacta agcaggcgcg gggaatttcg atcatcgtgc
    15841 cgaacaggta tttgattttt ttcaggccga actttttaag aacttcctga taaacttcat
    15901 ctacaattgc tttttggtgt tcgagttctt tagttaaact caccacggga atcattattt
    15961 ctggataggg ctttttacct tctttcagta attcggcaga cgcttccagg attgctcgaa
    16021 tctgcattcg ggaaatttcc ggataggtca ctcccagtcg aacaccgcga tgtcccatca
    16081 ttgggttaga ctcatgcagt tcagcggcac gcttttccag ttcatctaag gaaatattca
    16141 aatctctggc aagtttttcc tgcttttctt tttctcgggg gacaaattca tgaagcggcg
    16201 gatcgagcaa acggataacc accggatagc cgtccatcgc ctccagagtc gcctttatat
    16261 cctttctaac ataggggaag agttcatcaa gagccttttg gcgctcatca aatgtattag
    16321 atacaatcat ttttcgtaga gcaaagagtg gcgcgtcgct accttcgccg tagaacatat
    16381 gttccgtacg aaatagcccg attccttctg cgccgaattg acgagcgcgt ttagcatcgg
    16441 ctggagtgtc ggcgtttgta cggatagcca gacggcggac agaatcacag actttaagaa
    16501 attttaccag atcaggattt tcggtagcgt caatcatcgg tagagtcccg gcatatacat
    16561 aacccttagt cccattcaga gtaataaaat cgccttcctt aaaaacgcgg tcaccgattt
    16621 tcattacttt tttctgataa tctatatgaa tagcactgca accgacgata caacatttgc
    16681 cccatccgcg agcaacaagc gccgcatggc tggtcatacc accgcgagca gttaaaattg
    16741 cctgtgaggc gcgcataccg tccacatctt caggattggt ttcctcgcgt accaaaatta
    16801 cttttttgcc tgctttggcc cattcgacgg catcggctga agagaagaca atttgaccag
    16861 tggcggcccc gggaccagcc ggcaaacctt tggcaagcgg ttctattttt ttctcgtgtt
    16921 tcggatcaat aatagggtgc agtaattcat cgagttgaga aggagaaact cgcaagaccg
    16981 cttcttctct ggtgatgagt ttttcattca acatatctaa tgccatccgt acggcggcgg
    17041 gaccattacg cttaccgatg cggcattgga gcataaaaag gcgccccttt tcaatggtga
    17101 attcaatgtc ttgcatatca cggtagtgtt tttcgaggcg attccggtat tctacgagct
    17161 ctttataaat agccggcata tattcttcca gagtcacaag gtctttatta tgttcagaag
    17221 ccgaatattt atttattggt gctggggtgc gaataccggc tactacatct tcgccctgag
    17281 catttattaa atattctcca taaaattggt tttctccatt tccggggtta cggctaaaag
    17341 ctacgcctgt agcacagtcg tcccccatat taccaaagac cattgcctgg acagtcacag
    17401 cggtgcccca atcgtccgga atgccttcaa tacgacgata ggcaaccgcc cgtttgccat
    17461 tccaggaagc aaacacggct ccgataccac cccataactg ttcatacgga tcgtccggaa
    17521 aatctttgcc aagcactttc ttaacctgtt ctttaaacac ccgacatagc tctttcagat
    17581 catcggcggt aagatcagtg tcgctagtat agttttttgc ctttttgaaa tcgtccataa
    17641 ttttttctag ctgacgacga attccctcat cgtctttcgg ctcgatcccg gcggcttttt
    17701 ccataactac atcggaatac atcataatca aacggcgata ggcatcgtaa acgaagcgtt
    17761 cattgccgcc gctttgcttg attaagccgg gtatggtttt ttcggttaaa ccaatgttca
    17821 aaacggtttc catcatgccg ggcatagatt tgcgagcgcc ggagcggact gataccagta
    17881 agggattatc tgggtcaccg aatttacggc ccatgatttt ttccactttg gttagagccg
    17941 cttcgacttg ctccttcagt tccgccggat acttgcgatt atgttgataa taataggtgc
    18001 agacttcggt tgtgattgta aaaccgggcg gcaccggcaa gcgcagatcg ggatgaccag
    18061 ccatttcagc caagttagcg ccttttcctc ccaacaattc cttcatactt tcatttccat
    18121 cggctttacc accgccgaaa aagtagacat atttaggggt tttagatttt tgagttgtca
    18181 tttttttacc tcttcatgtt attctattaa ctgtaaaaaa tcagcgctaa gtttacgccg
    18241 aattgacttt tttttcaagt tttagttata ccgagtagat taatcttaag aataatgagc
    18301 cgggaaatta attgagcagg gtagaaaacg gcgctaaatt tttagatttt ttatccgctc
    18361 agcacgaatt aatgaaggaa aaagatgtct tgttaatctg atcgaagaac ctcgacggtt
    18421 ccttacaacg ctaatttttc ctgcagtacc ttgacaatat tctctttggg gagacggatc
    18481 tgctcgagtg tatcgcgatc gcgcaatgtt acactgccgt cttgtaaggt ctgcgaatcc
    18541 acggtaattc caaacggcgt cccggcttca tcctggcgcc gatagcgtcg tccaatggaa
    18601 ccggcttggt cataaaaagc cgcaaactgt ttttttacat cgttaaaaat ggcttccgcg
    18661 atttccggca ttccatcacg attgacgagc ggtaggatag cgactttggt cggagctatt
    18721 ttcggatcga gtcgcaagac gacgcggtca tgctcagcat cttcccagta agcatcacac
    18781 aaaaccgtca gaatggtgcg atctacgcca gcagaacttt cgacaacggc tggtacgata
    18841 tgcgtgttcg tttcgctatc aaaataagtc agcttcttgc cgctggcttt catgtgttgc
    18901 tcaaggtcga aagtttgccg atcggcgatg ccttccagtt ccgaccagcc gaagggaaat
    18961 ttatattcaa catcagtaca ggctttggca taatgggcca gttcttcttt ttcgtgcgga
    19021 cgccgccgca aattttccgc acggacaccg agttttttat accagttgaa gcgttcattg
    19081 acccaatact cgtaccagcg cccgctctct tccggtttga caaaaaattc catttccatt
    19141 tgttcaaatt cgcgggagcg aaaaatgaaa ttgccggtcg tgatttcatt gcgaaaagcc
    19201 tttccgatct gaccgacacc aaacggtagt ttgacacgcg tagtggaaat gacattatcg
    19261 aaattgacaa aaatgccctg cgccgtttcc ggccgtaaat agacttcaga agcagaatct
    19321 tccgatggac caaagtgggt tttgaacatt aaattgaatt ggcgggcggg tgatagttcc
    19381 gaactgccgc agttcgggca ggcgattttt tccaatttga tggttctctg gccgccgcaa
    19441 tggggacagt cggaatcgcg ataatgcgct atttcgcgag cgtataaatc gggaaaatgc
    19501 gccgcgaatt ccttttttac ttcaattaaa ccatcattga cgtaaaagtt aaaattacaa
    19561 ttggagcatt taaaataggc tggagcttta tcctcccgga aacgcgcctg acatttcttg
    19621 caatcaatca gtgggtcatg aaaattggcg acgtgcccgg aggcttccca gactttgggg
    19681 tgcatcaata tagcggcgtc cataccgacg acattttccc gactggtgac catatcatgc
    19741 caccacaaat ctttgacatt acgcttaagt tcgacaccga gcgggccata atcccaggta
    19801 ctgctcagcc cgccataaat atcactggat tgaaagatga tgccgcgccg tttacatagc
    19861 gagactaatt tatccatttg ttcgttacta acttgttttc ccattttttc cctaattttc
    19921 acatttcaat atttaattta caaatagttc agaaggaaat ttctaattaa gcaacatgtg
    19981 atttttcact cgtctgctgt ttcagttttg cattttgggc aaatcagcag aacactgcca
    20041 tcgcgtttaa tcttgcgttc caaaaattgg ctatcgcagt tctggcaggg gcgagccacc
    20101 ggttcattcc aggtagcgaa cttgcagttc gggtagcgat tacatccgta aaaaattttg
    20161 ccttttttgg tgcgcttttg aacgatttgc ccgtcgcaat ctggttccgg gcatttgata
    20221 ttcaaagtga atggttcggt cgttttacat ttaggatatt tggtgcaagc ccaaaatttg
    20281 ccgaaacgcc catctttaat tgtcatcgga ctgccgcagt tggggcaggt tttggattcg
    20341 gtcggcgcaa ccggcgcggt atttaacggc cgggtgtttt tacattcggg aaaagccgag
    20401 caggctaaaa aacgcccatt gcgactccat ttgatgacca tcggagcgcc gcagatctcg
    20461 catttttcat tagttttttc ctggaggccg gttttgattt cgcggcgctg ggcttggagc
    20521 tcttcgagcg atttatgaaa aggctgatag aattcgctga tgaccttttg ccattcttta
    20581 cgattgcctt caatgtcatc cagttcggct tccattcgag cggtgaactt gacattgaaa
    20641 atatcaggca taccatctac gagcaattta ttaacggttt taccgaggtc ggtcggtact
    20701 aggtaaccac ctttacgttc aacgtattta cgggtaataa tagtcgaaac gatggtggca
    20761 taagtgcttg ggcgaccgat accgagatta tctaactcct tgataagcgt accctcggta
    20821 aagcggggcg gcggttcggt aaatttttgc tccggttgga gttctaataa ggtgagccgg
    20881 tcatcttttt gtatattgat cggcagagca atttttttct cgtcctcggc tttttcctcg
    20941 cgccaagcac gcaggtagcc gtcaaaaatc aaatcactgc cggtagcgcg aaaaaggtaa
    21001 ttatcggctg tcacatctat aattttttgt ttgtaaacgg ccgcagacat ctgacaggcg
    21061 gcgaagcgat tccaaattag agtatagagc tttttctgtt ctggcgaaag gtagggagca
    21121 actacttccg gcgtgtattc aaaatgcgtg gggcgaattc cttcatgcgc gtcttgcacg
    21181 tttcttttag tagttttaaa atggcgcggg ttaggtggaa gaaactttgc gccgtaatgt
    21241 ttttcgatat attcacggat accggtgata gcttccttag aaatacgtac tgaatcagtg
    21301 cgcatgtagg ttatcaagcc gactggttca ccaccaattt cgacaccttc gtaaagattc
    21361 tgagcaatgc gcatagtttt ttccggcgag tagcgaaaac ggcgcgtggc ttcctgttgc
    21421 agggtgctgg tcgtgaaagg tggtgagggt gatttggtga cttctttctc ttcgatattg
    21481 ctgacttgat aggtagcatt tttcagaatc gctaaatgct tgttggcgtc ggatgcgttc
    21541 gaaatagcag tttttttatt gtcaatttta atgagcttgg cagaaaattt ttcgttttgg
    21601 ggtgtctgga gcagtgccgt tatttcccag tattcaagag gaatgaactg ctcgatttcg
    21661 acttcgcgtt cacaaatgat acgcaccgcg acggactgca cacgaccggc gctcaatcca
    21721 ctataaagtg tgcgccatag aaaagggctg acttggtaac cgaccagccg atcgaggacc
    21781 cgtcgggctt gctgggcatt caccaggctc atatctatct ggcgcggatg gctaagtcca
    21841 taacgaatgc cactttcggt tatttcattg aaaagaactc gttgaacgcg atcagatgga
    21901 acgtcaataa cattggctat atgccaggca attgcttcac cttcgcggtc tggatcaggg
    21961 gctaaataaa cacgttgagc agattgagcg gactctttca gattacttat aattttagat
    22021 ttgcctttga ttgtaacgta tttcggtttg aaatcgtgtt gaatatcaat accaagttct
    22081 ttctccggta aatctttgat gtgaccaacc gatgccatga ctttaaaatt gttaccgagg
    22141 tattttttga tagtcttggc tttagaaggc gattcgacga ttagtaattc catatcactt
    22201 tacttttctt aaggcggggt aaattacaaa tactgatcag aattccaagc atttttaaaa
    22261 taaaacttgt tcatttcaaa aatactcact gatattgaga cttccacaaa ttttaagaaa
    22321 aatacccgcg ctttttgatt atcagattag ttttcgcctc atcatccaat tttgttgaat
    22381 cacgaaaaaa agatgtttat ttccaccgct gaaagtaata atagtgaatt cagaaatacc
    22441 gaatcctgtt ttaggaaaag gctaaaatat gaacgcacaa aaactgacct gcacgtttta
    22501 agataggtaa gcacaataga ttttaataat ttttaaaaaa atgtaattta atatccagtt
    22561 aaagtaatat cattctgatt atcagcatat tagcagataa gttcgggaaa tattagggga
    22621 tatcctggtg cttggtgtat tgacattctg attataaata acttaaattt cacagctttt
    22681 ttaaaataat tgatccattc atgaaattga tctgggctta ctaacctcct gcgggagtga
    22741 tatagatggt gagtgtagct cagtatggta gagcgacagg ttgtggccct gttggccgcg
    22801 ggttcaagtc ccgtcactca cccgctcttt ttaatggcgc tatcgtctag tggttaggac
    22861 accacgcttt caatgtggta accggagttc aattctccgt agcgctacca atcgttccaa
    22921 gtcttttctt tagcctttat ttctttttaa aggcgctatc gtctacccgc ctattggtca
    22981 tgcgaccatt agtcgggcag ggtggttagg acaccacgct ttcaatgtgg taaccggagt
    23041 tccccgcctg acccgccagt gccagccacg cgggcaggaa ttctccgtag cgctaccaat
    23101 cgttccaagt cttttcttta gcctttattt cttnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23221 nnnnnnnnnn nnntattggt catgcgacca ttagtcgggc agggtggtta ggacaccacg
    23281 ctttcaatag taataatcaa atagtcaatt aattgagcgc ttttacaaac tttatgtttt
    23341 ttctcgtgat ttatttgttg aaattgatat aaaaagaacg tataatttta cggaatgaaa
    23401 tataatagat tattgattca caactatccc agactatagt cattatgcga ttgactaaaa
    23461 atcaacaaaa tgcacttcga gtagcccgtt taatctcggt aattctgcat ccactcgtca
    23521 tagcgccgct gacctatgct ttgattattg cgcatacttc cgaaatcaat cgaactcaaa
    23581 aattgctata tttagggtta gtgcttatcg ctatcatttt tatcccactc atttctgtct
    23641 tatacctaaa aaaaagaggg gagacgacca gcctggacgt tcctgaaaga gataaacgga
    23701 ttagtccctt tttagtctgt atcgcaggtt attttattgt atggattttg ttaaaagtgt
    23761 ctggggcacc gcgccctatt tctttactaa tgtggtgcta tggatttaat tcaattattg
    23821 ccactattat caccaaatac tggaaaataa gtgtgcatgg tatggcattg ggcggtccca
    23881 tagcggcttt aggctttgta atttcaccga ctttttactg gggtattttg atcgctccat
    23941 tgattgtcta ttcgcgagtg aagttgaagg ctcacactat cgctcaagta attacgggtt
    24001 ttctattagg atttttatta actttaatcc aattcgagat ttttttatga aaatcattga
    24061 cgttttggca attattctcg gcgggggcaa aggcacacgg ctttatcctt taaccaaata
    24121 tcgagccaag ccggctgttc ctttcggtgg caaattccgc attattgatg taccgattag
    24181 caattgcctc aatagcggaa tcaataaaat ctacattctt acccaattca atactcattc
    24241 gcttcatcgt catatttaca gcacttatag cttaggtccc tttagtaatg gttttgttga
    24301 tattttggcg gctcaacaga ctatggaaaa cactgactgg tatcagggaa ctgctgatgc
    24361 tgttcggaaa aaccttccgt tcatcaatga aatcaaagcc gcctatacta tgattctttc
    24421 tggtgaccac ctctaccgta tggattaccg tttgtttctg gaacatcata tcaagaataa
    24481 ggcggaaatt acggtagcgg tcaagccggt ttcacgacca gatgccggac atttcggcat
    24541 tttgcagacc gatgaaaaat gtcgtatagt tcaatttgtg gaaaaaccca attcggagct
    24601 tcttgataag ttaaaatctc ccggcttacc agacgagaca ccctttctag cttcgatggg
    24661 catctatatt tttgccacgg aaacccttca taaaattctc ggacaaaatc cgagcgacga
    24721 tttcggtaaa aatatcatac ccgcttcaat tcctacgctc aatgtctgtg cctatcaatt
    24781 tgatggctat tggcgggata ttggtaccat tgaatcgttt tttaccgcta atcttgaact
    24841 tgctagattc caaccgcctt tttctttata cgataaacac gctcctattt acactcatgc
    24901 ccgctcctta ccggcttccc gattagagaa ttgttcgctg actcaaactc tgctggcaga
    24961 cggctgtcat attggtgaga gcaatattag cgaatctgta gtcggtatta gaacaattat
    25021 ccagaaaggt accactattg agaaatgcgt cattatggga gctgattact tcgaacaggt
    25081 tcagccagaa g
//
LOCUS       DAOSMP010000033        24482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_2053_length_24482_cov_21.668277, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000033 DAOSMP010000000
VERSION     DAOSMP010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 24482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24482
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2053_length_24482_cov_21.668277"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PKX09_06785"
     CDS             complement(<1..841)
                     /locus_tag="PKX09_06785"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOU17561.1"
                     /translation="MKAGGIIIKKLIYLTITCILILSLSSPINAQVGLKKVAQSTMNF
                     LQVGVAPRASAMGEAYCALGIGAESIFCNPAGIAQSTHRFELQTYQTRWIADINYLAG
                     SFIWNLHQYGTLGLSMLSVDYGTIYGTSLLSAADKDLYPAGYIDLGNVDNVGAYAFGL
                     TYGRAISTQFLIGGSVRLVGQNLGQSQMADGLKDNNAVKLAFDAGVKYLTDFKSFRFA
                     MSIRNFSSNIKREEIDEQLPLLFTMGVAMDLLDVIAPPFSQNNSLNLAVDFQHPNNYS
                     ERIN"
     gene            complement(838..3000)
                     /locus_tag="PKX09_06790"
     CDS             complement(838..3000)
                     /locus_tag="PKX09_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17562.1"
                     /translation="MKNYRENNLRFNFSLILLAILCAGQFGFSQIQPELATALIKMGH
                     VWCGVTANGDKGSFEYRAGFFPNDYDIIAIRGQELDAWAGTGFRLAATNWQDPVDPDS
                     IHKVAIYGPTNDYMRTGKVIVPLTNYVRYTYPEQIINFNPINLEMFGTYDPSKFGEET
                     YDQIVEVTTQNILGVNIHRKILAWTQNYNDDYIIVDVTFTNVSEDTLHDFFINMESNG
                     VNAFRSNGSNPSPPSNERFNPTIVWQHYYGGRVGDSLRVFYEYSADDPTIAGDNMGAP
                     VTSQGGRLIGAKFCWYSILHASGQPYDDPANDRDDFTQPKVTYIGTATKIPYNDASDE
                     YGNKNYWAIRGGYSKYWPMIGDTIPGTFHGGNSDECGSPDYSAFPAGTRSSPNSKMSC
                     SFGPYTFAPGQEIHIVYASGYSGLDLPTAQEVGKKWLNGTLEDPPNLPDSRTGYLPSN
                     FVFPIDATEMDLRKDRWVSTGVDSVMKSARRAKWNFDNGYKIPQAPPPPTYLEITGLG
                     TGVEIKWRDPDAEAMPNFAGYRIMRRLSAMDTTFYQVIYDSGPDDKATDHLYVDRDVL
                     IGAQYYYYLQAKARIADDDPLADATTRGKIIYSSRVLVPNINWINPPHFSQTDLTKIR
                     IVPNPYNINDPLLAAQGWTDQRGIQFFNLPGKVTIKIFTENGDLVQVLKHDSPVEAGY
                     YFWDMITLSQQVISSGVYIVVFQKPNGETAFQKFLVVR"
     gene            complement(3028..6333)
                     /locus_tag="PKX09_06795"
     CDS             complement(3028..6333)
                     /locus_tag="PKX09_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU17563.1"
                     /translation="MLNNKPIIFFFCAALILTVGFTSNLQGQNRSRISGSVRDAETGE
                     PLIGVNIIIEETDLGAATDTDGNYIIINVPVGSHRVRASMIGYETKIFTNVMVSADRI
                     TRLDFELHRTVIQGQEVIVTAKRDELHKEVSTTQLVVDAMQISDATGIREINAFLAKL
                     PGIGEQNGYLTIRGGSADQTGMMVDGLSYVNAATGNAESSIPLSAIEQVSLLSGGYNA
                     EYGNFRSGLINITTKSGSSKDYHGTFSYSRDNAHIRRFGDSFYDAHNAALRSYLDPEV
                     AFIGTNTAWKDDAYLRDQYPSFTGWITQAQNYNIGKTEDQWATPFDYYLLACWMFMTI
                     PDYKGLANLSDSMKQVIGYQEVSKEQRKLFTQHHNREEYPDWNFDGGFGGPVPLIGKA
                     LGNATFYLSFNAKERHFVIPVSRHSDETYTTLLTMKSQPTKKTTLTYNGLYKYEDGVS
                     PIRPASGDYPDASRQGGFMPVNNIKYIHKNPEYWYDPPFFPIMSQRTAVNGITLNHII
                     NKTTFWELKLSALNIRDNSPTGNTRDMRAITHFGPFVVNEMPYGKLLDGTNRVTGIFN
                     GDTLSYKFPNYDALPGVAFRFRRKEGDLYNNVRVGQYGAKFDLSSQVGRHNFVKTGFE
                     YNYIDLDHNLWMMWNENAYNVYEFNYHRTPSQTGIYVQDQLNFESMIANIGLRFDYYY
                     GGGGKWPTGDPFATEAFTPQSYASDSVLFDYLKRGKSYIWDTWVRYDSLHPGFLQPIK
                     NHYALSPRIGISFPVTEQSKFYFNYGHFRSNPPYYSMYLYRYRYTKNGLYDMSNPNLE
                     PPRTISYELGIAMTLFQTSVLKMSWYSKDVTGQHGEVTYQNASGTINYNAWANNEYQD
                     IQGIEINFSKSDASWLTGWINFNYMLKKTGLTGRSLITDVTINNDQEGLYAGQETRSL
                     PQPKINANLTVRSFDKWGPKISGISPLANWTITLFAEWQAGEYFTWNPLNELHVNNNM
                     RWPDYYMVDLKVDKKFNLFGLAASFYVDISNLFNFKTTLMDDGYCFVREGGDLEEWSD
                     FVKYFGSLRLPMYESAKYDALRQQYPGMYIAGNDRIGELRSKEKPYINDPNYSFWLYE
                     QPRDIWFGLRFSF"
     gene            complement(6376..8013)
                     /locus_tag="PKX09_06800"
     CDS             complement(6376..8013)
                     /locus_tag="PKX09_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU17564.1"
                     /translation="MIKKYPGLILVMLIGVGGWKLLAQTIAFPGAEGSGRFTKGGRGG
                     EVYEVTNLNNSGPGSIVDAVSQSNRTVVFRISGTIELGSVVLKPKSNITIAGQTAPGD
                     GICLKGRIKISSNNIIIRYIRVRVDAGAANSSGDAIDIDKGKNIIIDHVSASYARDET
                     ISCQEASDSVTVQWCLMSEALTFEGHSYGSLVRGDFGDQKSYHHNLYAHCNGRLPRPG
                     NYTSLATDSLGLFFDFRNNVVYNWAGSSPGYNADKEPISRYNFIGNVYILGPESSTSS
                     KLFKEDAINAYAYFEDNMFNGVVPEDQWSLVKFNGFTTDQIKAYKQRSSLIPMEPVVT
                     TSPEQAYLDVLASAGCSFPKRDTIDCRIIQDVINTTGHSIDSTSAQPEGGWPLLSSLP
                     APTDTDHDGMPDDWEIAHSLNPADAADRNLLDENGYTRLENYLNGLVEQNPNRVVSKP
                     NVVADFRLAQNYPNPFNATTTIQFSLFKTGLAEIRIYDLAGQVVKTYPRQSLMPGEYR
                     LVWQGDNDAGKPVASGIYFCVMQFASNRQILKMQIIR"
     gene            complement(8082..9449)
                     /locus_tag="PKX09_06805"
     CDS             complement(8082..9449)
                     /locus_tag="PKX09_06805"
                     /inference="COORDINATES: protein motif:HMM:NF027642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU17565.1"
                     /translation="MKRRLLILFFVMSLAGLLNAQTFVAPGEGTLSAAISEAQDGDVL
                     ELLAGGEYTESALSKLGTIVNKSLTIRVDGEEKAIVRMLKSDLTADSAGVFFRLGNQA
                     SLILNGLELDGNSKIKYLVSFYMPVAQTPTLIKKIQIEDCYIHDITDDVMHGEKSSGI
                     QGYVIFDSTIINNSILHRIGTLLQYKQAGSNYIAVTNSTIATVSSYGFRACGCDASEG
                     SALPDHTPEVVIDHTTWYNIGTTDKREIILGEKGPLFRPWTITNSIFVKQCSSGGKKI
                     FINIKDNTTSNESCMGTITNICFWKIDKIAFYMHTVRDTIRMDPQFADTNNYDFTLPV
                     GSPLLTFGMDGKPIGDPRWGTNYVALKPAVENIPAKFGLASIYPNPFNPTTTISFGLA
                     ENGFTTLAIYDLRGNLVSQIVNEYLTAGNYNFTFDGSHLQSGIYLCRLTAGNQTNTRK
                     MVLLK"
     gene            complement(9742..11325)
                     /locus_tag="PKX09_06810"
     CDS             complement(9742..11325)
                     /locus_tag="PKX09_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOU17566.1"
                     /translation="MKKLSVFSLLFFLPVALAAFTGEIIKTYDLPSTCPTGLTYDGQN
                     LWLADRKTDQLYCLDKNDGKVVRQLPAPGYWPMGLTWDGKALWVSDLRDGKIYQIDPK
                     SGLVIKTIYAPGGSPRDLAWDGKYLWCADDGSDEIIQFSPDDGTTIQSFKAPSTEVRG
                     LAFDGHYLWADDRGKDEIYMIDPRSGCVLIVTTAPGPYPKGLAYDGQALWAVDYQTDK
                     LYQLKVQDDQKFIRKNERKAKVTYTHQTRNFGPGKIRNLDVYIALPQNRDSQKLNGNL
                     QFTPKYDKIVTDRWGQPAACFNLKNIEAGKTINLEMVTNVSVFEIQYFIYPEKVGGLE
                     TIPADIKQKYLENNEKYQYDHPIIQNAVREAVGYEKNPYWIARQIYNYLIDKMYYELV
                     GGWNTAPTVLERGSGSCSEYTFVYIAMCRAAGLPARYVGSVVVRGDDASLDDVFHRWV
                     EVYLPNYGWIPVDPSGGDNESPRDQAAYFGHLANRFLITTESGGASEMLGWFYNSNEF
                     FTTDPQTSVYIETFGEWEP"
     gene            complement(11351..11791)
                     /locus_tag="PKX09_06815"
     CDS             complement(11351..11791)
                     /locus_tag="PKX09_06815"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOU17567.1"
                     /translation="MKILIIHGPNLPFLGKISAGTGSRLTLDKVNTSLRRMAQESGIE
                     LKIFQFYSEEKILKVVARNRSDIAGLLISPGALAINCHTLRELLSILKIKTVEVHLSE
                     MPDAKASFGQSALRDIAVARFLEPGLTAYQKGLQYFIKTKDASQ"
     gene            11928..12065
                     /locus_tag="PKX09_06820"
     CDS             11928..12065
                     /locus_tag="PKX09_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17568.1"
                     /translation="MKIKKKILVLIIIFLLMVASLAISIRSNRQSDIPARESANLKIG
                     N"
     gene            complement(12133..12927)
                     /locus_tag="PKX09_06825"
     CDS             complement(12133..12927)
                     /locus_tag="PKX09_06825"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HOU17569.1"
                     /translation="MKIFKDNLLIILASVIAGLAGWQSCDLPWQPGPMPDAIIETEFV
                     PGLNILGIVRLEDTTASSFVYIERAYASNEYETWVDSLPIIKNANVWIAGTRDTTGYI
                     FRYYGEQSDHKYINADFRPVAGETYQLTITAADLPDLSASTSVPHLPAIDTGSVVVSE
                     DGVNFNLRTTADTYLYDVYLLCENGQLVNRFSNDGAIVKELQFKLNNNLDAPLAIQVY
                     GYDENMATILNATSSIIPQSFLETLSTVEGGYGCFGAVSVLTYVLP"
     gene            complement(12930..15242)
                     /locus_tag="PKX09_06830"
     CDS             complement(12930..15242)
                     /locus_tag="PKX09_06830"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU17570.1"
                     /translation="MKKIFSLWLILVTGLQAANISGYIVDEKTGEAIIGVNVLIANTG
                     LGTASDLRGFFVVRQVPAGLQTLRFSHISYAERELRIEVSDKALFLGTIKMTARALPT
                     SAVEVIGQRRTLIEKEMDIASFQVDPIVLLEAPQLRDDVFQLLKFSPSVSMSDPLSPQ
                     LHVRGSDPGENLVQLNGMTIYNPQHFLSSQAIFNPYSIKDIEMLVGGFDAEYGGRNSS
                     ILNITTREGHQSEVHGEFWPSTSGISGAIEFPVKKNATALLSGRLISDPTYRVLMNSP
                     NLMGDFVGSYLSKIGRTQLRLTAFYARDYLDYSLDNLMLYFPYDFYENFQEGLITDTE
                     NRAVGIQTHTLIRTNILLETHFFHSGSRADNDSYVNFVFPDTVNNLDRIIKYRTSIRN
                     SVQDYTAKASLSLFTFWNQTLKFGWEQSHLSFLNKIGGQTDTSARRIGADLQAVYIQD
                     KIEIGNLALKFGGRSSRQMSSESWHFEPRAALALRLGITTVKAAWGHYYQYLTTLDSR
                     NREFIQFLDYYYNLENQKPVESIHYILGVEGFLTDKLEYSVTGYYKDLTRLYRLEYDA
                     AKKSLELSEILEAGRGKAYGIEWLLRGEVERLSGWVSYTFSHGTRSYPSLQNGRWYYY
                     EGDQPHNLKLLLMYKLTPAITASTTFQFSSGYPRTWETGRYNQYFYNPLENSVGGFPR
                     DITPVKNNVRFPPRIVWDIGWKKRLREGFGQYLSEYLGLKEAYMTMTIRNLLFLRRNP
                     YYYFYFPGYGYYAFGLEYFPSVNFGYTLRF"
     gene            complement(15239..16261)
                     /locus_tag="PKX09_06835"
     CDS             complement(15239..16261)
                     /locus_tag="PKX09_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17571.1"
                     /translation="MISLTLLLGLCFSNALAVKFSWSELISDTLYISTYADALTLTGF
                     PAIPVNDERAESPTILGIQQIKKWHYIPVDQYIALNEPLEIQMSKFFQKFQVTGNQTL
                     VIDHLSLWYDASPAFGAARTLNGYTRLVDSSGETVRDWQWELREKSAKNQKPADNIAI
                     LLNKWMIAQTESLQRRPASDLPVSPYRYRRQMIPWTDIISLGDGYIVIAHLALEYPAD
                     QMVKFIRGSPGIYYRKSSKHESIAIGGRDQQWYFRLNRSWLARLNTTVRVGFNNFNPR
                     EYDYVDFWNIMLINLGFNASLEYRPIYHKGFFVGAGLHQAVNLLPSVINTLETGLLFS
                     IGLVLP"
     gene            complement(16470..16640)
                     /locus_tag="PKX09_06840"
     CDS             complement(16470..16640)
                     /locus_tag="PKX09_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17572.1"
                     /translation="MLIYFLLILGCATLAFALMMAALKFSRYKGEKHSGQCGAECHCS
                     YEKKRTCVKPNQ"
     gene            complement(16693..17922)
                     /gene="nqrF"
                     /locus_tag="PKX09_06845"
     CDS             complement(16693..17922)
                     /gene="nqrF"
                     /locus_tag="PKX09_06845"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HOU17573.1"
                     /translation="MSSLVIILIAVGVFIFIVLALVGLILLAKSYLVPVGTIKININD
                     DPDYSFDAPVGGKLLNTLAEKEIYLPSACGAAGTCGLCKVQVLKGGGTILPTELAHIN
                     RREARAGVRLACQLAVKEDLWLSIPTEIFAIRKWICTVRSNRNVATFIKELVLDLPPG
                     EQINFRAGGYIQVETGPHHVNFKDFVIEEPFKSEWDRCNMWRYVSIVDEEVVRAYSIA
                     NYPDEKGIIMLNVRIATPPPEKPDAPPGKMSSYLFSLKAGDRVTISGPYGEFFAKETD
                     REMVFIGGGAGMAPMRSHIFDQLKRLHTQRKISFWYGARSLKELFYQEDFDALQREFP
                     NFEWHVALSEPLPEDNWNGYCGFIHEVVLNNYLINHPAPEDCEYYLCGPPLMVSAVLK
                     MLDDLGVEPENILYDEF"
     gene            complement(17936..18544)
                     /gene="nqrE"
                     /locus_tag="PKX09_06850"
     CDS             complement(17936..18544)
                     /gene="nqrE"
                     /locus_tag="PKX09_06850"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011396546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HOU17574.1"
                     /translation="MLEHYLSLFIKSVFIENMALAFFLGMCTFLAVSKRLETAVGLGI
                     AVVVVQTITVPINNLIYHNLLAEGALSWLGIDLDLTFLGLITYIGVIAAVVQILEMVL
                     DKFVPKLYHSLGIFLPLITVNCVILAGSLFMVERDYTFGESVVFGIGSGIGWALAIVA
                     LAGIREKLKYSNVPSGLRDLGITFITAGLMAMAFMLFSGIQL"
     gene            complement(18548..>18699)
                     /locus_tag="PKX09_06855"
     CDS             complement(18548..>18699)
                     /locus_tag="PKX09_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421083.1"
                     /note="Part of the NQR complex which catalyzes the
                     reduction of ubiquinone-1 to ubiquinol by two successive
                     reactions, coupled with the transport of Na(+) ions from
                     the cytoplasm to the periplasm; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOU17575.1"
                     /translation="LLGFTILRVNTEGGWYVPNGLLVLPASAFFLIGLIIWGLHTLKP
                     ETKQE"
     assembly_gap    18700..18799
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18800..19320)
                     /locus_tag="PKX09_06860"
     CDS             complement(<18800..19320)
                     /locus_tag="PKX09_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008758703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOU17576.1"
                     /translation="MNKSLTTLTTPIIKENPVIVQVLGICSALAVTSKLETAIVMALA
                     VTFVTAFANMAVSLIRRQVPGSIRIIVEMTIIASLVIVADQLIKAFAFGVSKQLSVYV
                     GLIITNCILLGRTEAFGLKNAPLASFLDGLGNGLGYSLILLIVASIRELFGTGKLLGF
                     TILRVNTEGGWYVP"
     gene            complement(19313..20113)
                     /locus_tag="PKX09_06865"
     CDS             complement(19313..20113)
                     /locus_tag="PKX09_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HOU17577.1"
                     /translation="MSDESARKTFLVALGVCLICAVMVATSVVLLRPRQQVNQELDKL
                     KNILQAANLYTDDAAVKKTFQEKIRPLLVELATGQIISETPSDKRLDPARFDSKAIAR
                     DPALSRTTPSADDLAHIGRQPKYMVVYEVVGNDQAGGVILPVYGKGLWSTLYGFIAFG
                     PDLQTITGFIIYEHGETPGLGGEVDNPLWRAKWVGKRAFDDSGQLKIEVIKGKVDPTS
                     PQSQYQIDGLSGATLTTRGIDQLVKFWLGENGYGPFLRNLQAKRMRNE"
     gene            complement(20103..21305)
                     /locus_tag="PKX09_06870"
     CDS             complement(20103..21305)
                     /locus_tag="PKX09_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019018052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HOU17578.1"
                     /translation="MGGLRKFLDQAGKPFRPGGKLDGLYYLFEAIDAFFYTPGKITKG
                     TVHVRDFMDLKRMMITVFVALIPTVFMAFYNTGLQANLVLQSLGSGANFTWQQRLMLA
                     LGLDFNPESFWSNFIHGGLYFLPVYIITLIVGGFWEVLFAVIRKHEVAEGFFVTSLLF
                     PLILPPTIPYWQVALGISFGVVLGKEVFGGVGFNVFNPALVARAFLFFAYPAQITGDK
                     VWIAVDGISRATPLAQFAETSSAVTTSWLDAFLGFIPGSMGETSTLACLIGALILLVT
                     GVGSWRIMLSILIGMVTLSTTFNLMGSATNPMFAVTPLWHLVLGGFAFGTVFMATDPV
                     SGAVTKPGQWVYGLLIGVMVIMVRVLNPAFPEGMMLAILFGNMFAPLIDRIFINAHIK
                     RRATRDVR"
     gene            complement(21305..>21946)
                     /locus_tag="PKX09_06875"
     CDS             complement(21305..>21946)
                     /locus_tag="PKX09_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020408770.1"
                     /note="uses the energy from reduction of ubiquinone-1 to
                     ubiquinol to move Na(+) ions from the cytoplasm to the
                     periplasm; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit A"
                     /protein_id="HOU17579.1"
                     /translation="KTVWSIDAQDVAAIGKLFTTGRLPTARIVALAGSAVKKPRLITT
                     RVGANLSELTDGELAEGHNRIISGSVLSGHTAAGELNFLGRFHQIVSVIPEGGRRKLF
                     GWLSLGARLFSVKNIALAAFLPNRKLVFDTDTHGSERAIVPIGSYEKVMPLDILPTYL
                     LRALAVDDLEEAEKLGCLELVEEDLALCTFVCPSKIDHGQNLRRVLNLIEKEG"
     assembly_gap    21947..22046
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22047..22806)
                     /locus_tag="PKX09_06880"
     CDS             complement(<22047..22806)
                     /locus_tag="PKX09_06880"
                     /inference="COORDINATES: protein motif:HMM:NF017689.2"
                     /note="uses the energy from reduction of ubiquinone-1 to
                     ubiquinol to move Na(+) ions from the cytoplasm to the
                     periplasm; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit A"
                     /protein_id="HOU17580.1"
                     /translation="MLIKIKKGLNLPIAGAPEQVIYPAQSVSQVALLGLDYVGLKPQF
                     AVTIGDQVKLGQPLFSDKTYPQIRFTAPGTGQVIAINRGAKRAFLSLVIKLDGDDAVE
                     FKSFSEQQIGSLTREEITNQLLESGLWPSFKARPFGKLANPESQPHSIFVTAMDTNPL
                     APDVAVILREQANDFQRGLALIAKLTAGKVFVCQAPDTAIPIPEIENIEIVEFAGPHP
                     AGLVGTHIHFLDPVSLHKTVWSIDAQDVAAIGKLF"
     gene            complement(23191..>24482)
                     /locus_tag="PKX09_06885"
     CDS             complement(23191..>24482)
                     /locus_tag="PKX09_06885"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOU17581.1"
                     /translation="QGWRLACQCQVTEPLVIELAQLEISILADATRIKPAGEDSLAVA
                     VDLGTTTLVAQLLDLSSGIVLGVETALNPQARHGADIMSRIQFGLTTAGRELLQNLIR
                     HQIGQMIANLVEKCRCPERRLKQINIVGNSVMHHLFGGLSVEPLALLPFEIEQPGELE
                     WSAEEISWQLPGNPKVKFLPCLGGFVGSDILAGILAVGMHRSPETVALIDLGTNGEIV
                     IGNRDRILCASTAAGPAFEGAQITMGMRATTGAIWKVWLTNGQLQVEVIGNVPARGIC
                     GSGLVDAVAASLDLDWIDPTGRITLPEREIPLQTPVVVTQADIRELQLAKGAIAAGIQ
                     ILRKQMQLKPADIKQVFLAGAFGNYINVVSARRIGLFDFPTELIEQAGNTALHGAKLS
                     LLNEQEYHSIPLITKHFPLATDAQFMEIFAENMNFPG"
BASE COUNT         6641 a         5813 c         5525 g         6303 t
ORIGIN      
        1 aattaatccg ttcggaatag ttattcggat gctgaaaatc aaccgctaaa ttgagtgaat
       61 tattttgcga gaatgggggc gctatgacat ccagcaaatc catcgcgact cccatcgtaa
      121 aaagcaaagg taattgttcg tctatttctt cccttttgat attagatgag aaattgcgaa
      181 tagacatcgc gaaccgaaag ctcttgaaat ctgtcaggta ttttacccca gcatcaaacg
      241 ccagtttaac cgcattatta tctttcaagc cgtcagccat ttgactttga cctaaatttt
      301 gaccgacgag acgtacactg ccgccgatta aaaactgggt gctgatcgcg cgaccataag
      361 ttaaaccaaa cgcataggcg ccgacattat caacattgcc caaatcaata tagcctgccg
      421 gataaaggtc tttgtccgcc gccgacaaga ggctggtacc ataaattgtc ccgtaatcaa
      481 ctgaaagcat actcaatccc agcgttccat actggtgcaa attccagata aagctcccgg
      541 caaggtaatt gatgtcggca atccagcggg tctggtaagt ttgcagttcg aaccgatggg
      601 tggattgggc aatgccggcc ggattgcaaa aaatgctttc tgcgccgatt ccgagagcgc
      661 aataggcttc gcccatagcg ctggcacgcg gtgcgacacc cacctgtaaa aagttcatcg
      721 tactctgcgc tactttcttc agcccgactt gagcattgat tggcgaggaa aggcttagga
      781 tcaaaataca agtaatagtc aaataaatca gttttttaat gataattcct cccgccttca
      841 tcgtaccacc agaaatttct gaaaagccgt ttcgccgttc ggtttctgaa acacaacgat
      901 ataaacacca ctactgataa cctgttgact gagagtaatc atatcccaga aatagtatcc
      961 ggcttccacc gggctatcat gtttgagaac ctggaccagg tcgccatttt cggtaaaaat
     1021 tttaatcgta accttgcctg gtagattaaa gaattgaatg ccgcgctggt cggtccagcc
     1081 ctgggcggca agtaaaggat cgttgatgtt gtaaggattg gggacgatac ggattttagt
     1141 caaatcggtt tgcgaaaagt gagggggatt aatccaatta atattcggca ccagcacccg
     1201 cgaactgtaa ataattttac cacgagtcgt tgcatctgcc agaggatcat cgtcggcaat
     1261 ccgggcttta gcctgcagat agtaataata ctgcgcgccg attaagacat cgcgatcgac
     1321 atacaggtgg tcagtggctt tatcatcagg accggaatca taaatcacct gatagaaggt
     1381 ggtatccatt gcgctcaggc ggcgcataat ccggtagccg gcgaagttcg gcatagcttc
     1441 ggcatctggg tcgcgccatt ttatttcgac cccagtaccc aaaccggtga tttcgagata
     1501 agttggcggc ggcggagcct gaggtatttt gtagccgtta tcaaagttcc atttagcacg
     1561 tcgggcgctt ttcataactg aatcaacgcc ggtactaacc cagcggtctt tgcgcaaatc
     1621 catttcggtc gcatctattg gaaaaacaaa attggatggc aaataaccgg tgcgagaatc
     1681 cggtagattg ggcggatctt cgagagtgcc attaagccac tttttgccaa cttcctgagc
     1741 ggttggcaga tctaagccgg agtaaccgct ggcgtaaacg atgtgaatct cttgcccggg
     1801 agcaaaagta tagggcccga aactacagga catcttcgaa ttgggactgc tcctggtacc
     1861 ggctggaaag gcggaataat cgggactacc gcattcgtcg ctgttgccgc cgtggaatgt
     1921 tccgggaatt gtatccccga tcattggcca gtattttgaa tagccaccgc gaattgccca
     1981 gtaattctta ttaccatatt catcgctggc gtcgttataa ggaattttgg tcgcagtgcc
     2041 aatgtacgtt actttcggtt gggtaaaatc atcgcggtcg ttggccggat catcatacgg
     2101 ttggccggaa gcgtgcagga tcgaatacca gcaaaacttt gccccgatca aacgcccgcc
     2161 ctgagaagta accggcgcac ccatattgtc gccggcaatt gtcggatcat cggcgctgta
     2221 ttcgtagaat acccgcaacg aatccccgac ccgaccgccg taataatgtt gccagacgat
     2281 tgtagggtta aagcgttcat tagagggcgg cgaaggattc gaaccattgg aacgaaaagc
     2341 attgacgccg ttcgattcca tattgataaa aaagtcgtgg agcgtatcct cgctgacatt
     2401 ggtgaaagtg acatcgacga tgatatagtc gtcattataa ttttgagtcc aggctaaaat
     2461 cttgcgatga atattgactc ccagaatgtt ctgagtggtt acctcgacga tttggtcgta
     2521 agtttcttcg ccgaatttac ttgggtcgta ggtcccaaac atttctaaat taatgggatt
     2581 aaaattaatt atttgttcgg gataagtata acggacataa ttggtcaatg ggacaatcac
     2641 tttgccagtg cgcatataat cgtttgtggg cccataaata gctactttat gtatggagtc
     2701 gggatcaacc gggtcctgcc aattggtagc cgccagacga aaaccggtac ccgcccaagc
     2761 atcaagttcc tgtccccgaa tagcgataat atcgtaatca ttagggaaaa agccagcccg
     2821 atattcgaaa ctacctttgt cgccattggc ggtcaccccg caccaaacgt gtcccatttt
     2881 gattaaagcc gtggcgagtt ccggctgaat ctgggaaaag ccgaattgac ccgcgcataa
     2941 tattgccagc aagatcaaag aaaaattaaa cctcaggttg ttctctctgt aatttttcaa
     3001 gtcaattact ccttcaaaaa tcaatcctta aaaactaaac ctcagaccaa accaaatatc
     3061 gcgcggttgt tcgtagagcc agaatgaata gttgggatcg ttgatatatg gtttttcttt
     3121 tgagcgcaat tcgccgatcc ggtcattgcc ggcgatatac attccgggat attgctgacg
     3181 aagagcgtca tatttggcag actcgtacat cgggagacga agcgagccaa aatatttgac
     3241 gaagtcactc cattcttcca gatcaccacc ttctctgaca aaacagtatc catcatccat
     3301 taaggtggtc ttaaaattga ataaattact gatatcaacg tagaaagagg ctgctaatcc
     3361 gaacaagtta aacttcttat caacttttaa atctaccata taataatcag gccagcgcat
     3421 attgttgttg acatgaagtt cgttcagcgg attccaggta aaatattcac cggcttgcca
     3481 ttctgcgaag agagtaattg tccaattcgc cagcggggaa atacctgaaa tttttgggcc
     3541 ccatttgtcg aatgatcgca cggttagatt ggcattaatt ttaggttgtg gtaaactacg
     3601 cgtttcctgg ccagcatata gtccttcctg atcgttattg atggtaacgt cggtaatcag
     3661 gcttcgtcca gtcaagcccg tcttttttag catataattg aaattgatcc agcccgtcag
     3721 ccagctggca tcacttttgg aaaaattaat ttcaattccc tgaatatctt gatattcgtt
     3781 gttagcccaa gcattgtaat tgatcgtgcc actggcgttc tggtaggtta cttcaccatg
     3841 ttgtccggtg acatctttgg aataccagga cattttcaaa acgctcgttt gaaaaagtgt
     3901 catcgcaatt cccaattcgt aggaaatcgt acggggcggt tccagattgg gattggacat
     3961 atcatatagc ccgtttttgg tataacgata gcgatacagg tacatcgaat agtaaggtgg
     4021 attggagcgg aaatgtccat agttgaaata aaatttcgac tgttccgtga ctgggaagga
     4081 aatcccaatg cgtggactca aagcataatg atttttaatc ggttgcagga agccgggatg
     4141 gagtgaatca tatctaaccc aagtatccca gatgtaagac tttcctcttt tcaaataatc
     4201 aaaaagaacc gaatcggacg cataactctg gggggtaaaa gcttcggtag caaatggatc
     4261 gccggtaggc catttacccc cgccgccgta ataatagtca aagcgcaaac cgatattggc
     4321 gatcatagat tcaaaattca attggtcttg aacgtagatc ccagtctggg atggagtgcg
     4381 atgataattg aattcgtaaa cattgtacgc gttttcgttc cacatcatcc atagattatg
     4441 atctaaatct atatagttat actcaaagcc cgtcttgaca aaattgtggc gtccgacctg
     4501 agaagataga tcgaacttcg ctccatactg gccgactcgc acattattat acaaatcgcc
     4561 ctctttgcgc cggaagcgaa aagcgacgcc cggcagagcg tcataattag ggaatttgta
     4621 actgagagta tcgccgttga atattccggt gacgcgattt gtcccgtcga gtaatttccc
     4681 gtacggcatt tcattgacta caaagggtcc gaagtgggta atggctctca tatcgcgcgt
     4741 gttgccagtc ggagaattgt cccgaatgtt caaagcgctg agtttgagtt cccagaaagt
     4801 tgttttgttg ataatgtggt tcaaagttat accgtttacc gcagttcttt gactcataat
     4861 agggaaaaaa ggtgggtcgt accaatattc gggattttta tgaatatatt tgatgttatt
     4921 gaccggcata aatccaccct ggcggctggc atcgggataa tcaccggaag ccggtcgaat
     4981 cgggctaacc ccgtcttcgt atttataaag gccattatag gttagagtgg tcttcttagt
     5041 cggttggctt ttcatagtta gtaaagtcgt ataggtctcg tcgctgtggc gcgaaaccgg
     5101 tataacaaag tggcgctctt tggcattaaa tgatagataa aaagtagcgt tacccaatgc
     5161 cttgccgatc agaggcaccg gaccaccaaa accgccgtcg aaattccaat cgggatattc
     5221 ttctcggttg tgatgctggg tgaagagttt cctttgttct ttagatactt cttggtaacc
     5281 gataacctgt ttcattgaat cggaaagatt agccaatcct ttataatccg gtatggtcat
     5341 aaacatccag caagctagca ggtaatagtc gaacggagtc gcccactggt cttcggtttt
     5401 accgatatta tagttctggg cttgagtgat ccagcccgta aaactgggat actgatcacg
     5461 taggtaagca tcgtctttcc aagcggtgtt tgttccaata aaagccacct caggatcaag
     5521 gtaagagcgc aaagccgcat tatgagcatc gtaaaatgaa tcgccgaacc ggcggatatg
     5581 ggcattatca cgagagtaac tgaaagttcc gtgatagtct ttggagcttc ccgacttggt
     5641 cgtgatgttg atcaatcccg aacggaaatt gccgtattcg gcattataac cacccgaaag
     5701 taaagagact tgttcgatag cgctcaacgg gatactgctt tcggcattgc cggtggcggc
     5761 attgacgtag gataagccgt caaccatcat cccggtttga tccgccgaac cgccccgaat
     5821 agtcagatag ccgttttgtt caccgatacc aggaagttta gccaggaagg cgttgatttc
     5881 ccgaatgccg gtagcatcgc tgatctgcat cgcatcaaca accagctggg tggtggaaac
     5941 ctctttgtgc agttcatcac gcttagcagt aacaataacc tcctgcccct ggatgacggt
     6001 gcgatgcagt tcgaaatcga ggcgagtaat ccgatcggca gaaaccataa catttgtaaa
     6061 tattttggtc tcgtaaccta tcattgaagc ccgcacgcga tgagagccga ccggtacgtt
     6121 aatgatgata taatttccgt cggtatctgt ggcggcgccc aaatcagttt cttcaatgat
     6181 aatattaact ccgatcagcg gttcaccggt ctcggcgtcg cgtacactgc cgctgattcg
     6241 gctgcggttc tgcccctgta aattactcgt gaatccgact gtaaggatta aagcggcgca
     6301 gaagaaaaaa attatcggct tgttgtttaa cataactact cctaaagccg tgtgttattt
     6361 tccaacatta tttttttaac gaatgatctg cattttcaaa atttggcgat tactagcaaa
     6421 ttgcattacg cagaaataaa ttccggaagc cactggttta ccagcatcat tgtcgccttg
     6481 ccataccaac cggtattcgc ccggcatcag actttgccgc ggataagttt tcacaacctg
     6541 accggcaagg tcataaatcc tgatttcagc cagcccggtt ttgaataatg aaaactgtat
     6601 ggtcgtcgtc gcattgaagg gattgggata attttgggca agccgaaaat cagcgactac
     6661 attcggtttg gaaacaaccc gattaggatt ctgctcgacc agaccgttca aataattttc
     6721 caaacgggta tagccattct catctagcaa attgcggtca gcggcatcgg cgggattcaa
     6781 actatgagca atttcccagt cgtcgggcat accgtcatga tcagtgtcgg ttggcgccgg
     6841 caaactgctt agaagaggcc aaccgccttc gggttgggcg ctggtactat caatcgaatg
     6901 cccggtagtg ttaatcacat cctgtatgat ccgacagtca atcgtgtcgc gttttggaaa
     6961 actgcaaccg gcggaagcca ggacgtcaag ataagcttgc tccggcgagg tcgtcacgac
     7021 tggctccatc ggtattagag aagagcgctg tttgtaagcc ttgatctggt cggtagtaaa
     7081 accgttgaat ttgaccaaac tccactggtc ttccgggacg acgccgttaa acatattatc
     7141 ttcaaaataa gcataagcgt taatagcgtc ttctttaaat agtttactac tggtgctgga
     7201 ttctggccct aagatataaa cattgccgat gaaattgtaa cggctaattg gttccttgtc
     7261 agcattatat cccggggatg aaccagccca attgtaaacc acattattgc ggaaatcgaa
     7321 aaataaaccg agcgaatcgg tagccagaga agtataattg cccggtcgcg gcaaacgccc
     7381 gttacaatgg gcatataagt tgtgatggta gctcttctga tcaccaaaat cgccgcgcac
     7441 gagtgatcca taactgtgtc cttcgaaagt cagggcttcg ctcatcagac accattgcac
     7501 agtaacacta tcggaagctt cctggcagga gatcgtttca tcgcgggcat aggaggcgct
     7561 gacgtggtca atgatgatat ttttaccttt atcaatgtca atggcgtcgc ccgaagaatt
     7621 agccgccccg gcgtccaccc gcacccggat ataacgaatg attatattat tactactaat
     7681 tttgattcgt cctttgaggc aaatcccgtc gcccggcgcg gtctgaccag ctatcgttat
     7741 attggactta ggcttgagaa cgacgctccc cagctcgatt gtcccggaaa tccgaaaaac
     7801 gaccgtgcgg ttggattggc tgacggcgtc aacaattgat ccgggtccgc tgttgtttag
     7861 attggttacc tcataaacct caccaccgcg cccacctttc gtaaagcgcc cacttccttc
     7921 cgcaccgggg aaggcgattg tctgtgccag tagcttccag ccaccgacgc cgattaacat
     7981 aaccaggatt aatcccggat atttttttat catttaagcc tctgaattac aaaacttagc
     8041 cggttgtcaa tgccccaaac aattttaaca attaccattg gttacttcag gagaaccatt
     8101 tttcgggtat tagtctgatt gccagcagtc aggcgacaga gataaatacc cgattgcaga
     8161 tgactgccat cgaaggtaaa attgtagttg ccagcggtca aatattcatt cactatttga
     8221 ctgactaaat taccgcgcag gtcgtaaatc gccagcgtcg tgaagccatt ttcagctaat
     8281 ccgaacgata tagtcgtcgt cggattgaat ggattggggt aaattgatgc cagcccgaac
     8341 ttcgcgggaa tattctcgac cgccggtttt agtgcgacat aattcgtgcc ccagcgcgga
     8401 tcaccgatcg gcttaccgtc catgccaaat gtcagtagcg gtgaaccaac cggtagggta
     8461 aaatcataat tattcgtatc cgcaaattgg ggatccatcc gaatcgtatc tctgacggta
     8521 tgcatataaa aagcaatctt gtcaattttc cagaaacaga tatttgtaat cgttcccatg
     8581 caggattcat tgctagtagt gttatcttta atgttaataa atattttttt ccctccactg
     8641 gaacactgtt ttacaaaaat agaattggtt atagtccacg gtctaaaaag tggtcccttt
     8701 tcacccagaa tgatttcgcg cttatcagtt gtccctatat tataccaagt agtatgatca
     8761 atcacgactt cgggggtgtg atctggtaag gccgatcctt cgctagcatc acatccacaa
     8821 gcccgaaagc cataggatga aaccgtagcg atggtggaat tagttactgc aatatagttt
     8881 gatcccgcct gcttatattg aagaagggta ccgatccgat gaagaattga attattgata
     8941 atggtcgaat cgaaaattac atagccttga ataccgctac ttttctcgcc gtgcataacg
     9001 tcgtcggtaa tatcgtggat gtagcagtct tcgatctgaa tttttttaat taaggtcgga
     9061 gtttgcgcta cgggcatata aaatgaaacc agatacttta ttttggaatt accatctaat
     9121 tccaaaccgt tcaatatcaa cgaagcctga ttgcccaatc taaaaaacac cccagcggaa
     9181 tcggcggtca gatcgctctt caacatccgg acgatcgctt tttcttcgcc atccacgcgg
     9241 atcgtcaggc tcttgttaac tatagtcccc agttttgaga gagcggattc ggtatattcg
     9301 ccaccagcca ataattccag gacgtcgccg tcctgtgcct cgctgatcgc cgcggaaagc
     9361 gttccctcac ccggcgctac aaatgtctga gcatttagca atccagctag gctcattacg
     9421 aaaaagagaa taagtagcct tcttttcata tttatcctcc tttttttact aaaagttatc
     9481 atacaaatat tccatgttgt aaactaaaaa atatgtatga caagtgcaag cttaccggag
     9541 ccgcgaatta ccctaatttc ttagaaactt gaacctttct gatttaaata ttcgtcgttt
     9601 aaaaaaattc tacaataatc taagatttag agtctatttt gaatttattg tttataaact
     9661 gtcacatctt atagtgatca agttattgta gatcttttat caaatcgcct aacatacacc
     9721 gaacccccca ttgaacccat atcacggttc ccactcgccg aaagtctcaa tataaacgct
     9781 ggtttgcggg tcggtggtaa aaaattcgtt ggaattgtaa aaccagccga gcatctcgga
     9841 ggcgccgccg ctttcggtag taatcaggaa gcggttagcg aggtgaccga agtaagccgc
     9901 ctggtcacgc ggggattcgt tatcgccacc gctcggatca accggaatcc agccgtaatt
     9961 cggtaagtaa acttcgaccc agcgatggaa gacatcatcc agactagcat cgtcgccgcg
    10021 cacgacgacc gagccgacat aacgcgccgg caatccagcc gcccgacaca tagcgatgta
    10081 aacgaaagtg tactcggaac aactgccact gccacgttcc agaaccgttg gagcagtatt
    10141 ccagccacca accagctcgt agtacatttt atcaatcaag taattgtaga tttggcgggc
    10201 aatccagtaa ggattttttt cataacccac cgcctcgcga acggcatttt ggatgatggg
    10261 atgatcatat tgatatttct cgttgttttc gagatatttc tgtttaatgt cggccggaat
    10321 tgtttccagt ccgccgactt tttcgggata gataaaatat tgtatttcaa aaacgctcac
    10381 attagtcacc atctctaaat tgatggtttt accggcttcg atatttttca gattgaaaca
    10441 tgccgctggc tgtccccaac gatcggttac gattttatca tatttgggtg tgaattgcag
    10501 attgccgttg agtttctggg aatcgcgatt ttggggcaga gcgatataga cgtcaagatt
    10561 acgaattttg cccggcccga aattgcgagt ctggtgggta taagttactt tagccttacg
    10621 ttcattttta cgaatgaatt tttggtcgtc ctgcactttc agctgataga gtttatcggt
    10681 ttggtagtca accgcccaga gcgcttgtcc gtcgtaagcc agacctttcg ggtatggtcc
    10741 tggagcagtc gtaacaatga gaacacagcc actccgcgga tcaatcatat agatttcatc
    10801 tttgccgcgg tcatcagccc aaagataatg accgtcaaaa gccagaccgc ggacttcggt
    10861 agaaggggct ttgaaggact ggatagttgt gccgtcgtcc ggactgaact ggataatttc
    10921 atccgagccg tcgtcggcgc accacaggta ttttccgtcc caggctaagt cgcgcggact
    10981 cccgcccggt gcataaatag ttttaataac caatccgctc ttcggatcaa tttggtaaat
    11041 cttgccatct cgtaaatccg agacccagag cgcttttcca tcccaggtta aacccatcgg
    11101 ccagtagccg ggcgcgggca actgacgcac gaccttaccg tcgtttttat caaggcaata
    11161 aagttggtcg gtttttcgat cagcaagcca gaggttttgg ccatcgtaag tcagtcctgt
    11221 gggacaggtg gacggtaaat cataagtctt gatgatttcg ccggtaaaag ccgctaacgc
    11281 aaccggtaag aaaaataata ggctaaaaac gctaagtttc ttcataaaaa acctccagta
    11341 ttgacaggtt tcattgtgac gcatctttgg ttttaataaa atattgtaag cctttttgat
    11401 aggcagttaa accgggttca aggaaacgcg cgacggcgat gtcccgcaaa gcggattgac
    11461 caaagcttgc cttcgcgtct ggcatttcgc tcaggtgaac ctcgacggtt ttgatcttca
    11521 gaatggaaag cagttcgcgt agagtatgac aattgatggc cagagctccc ggactgatta
    11581 gtaatccggc gatgtcggaa cggttacgag cgacgacttt caatattttt tcttcggaat
    11641 aaaattgaaa tattttcagt tcaatgccgg attcctgtgc catacgcctc agggaagtgt
    11701 tgactttatc taaggtcaag cgcgagccgg ttccagctga gattttaccc agaaagggca
    11761 gattgggtcc gtgaattatt aatattttca tactgaaaaa taagcgggat ttggctgaat
    11821 tgcaaatgtg atgaaagtca agttacgtcg cgagtttgtc gcttgacaca gattaactgc
    11881 caccggttca ttaaaattta agtatcttga attgacacag tagatttatg aaaataaaga
    11941 aaaaaatatt ggttctgatt atcatttttt tgctgatggt agcgtctctt gctatcagca
    12001 tccgcagtaa tcggcaatcg gacatccccg ccagggaatc cgccaatctc aaaatcggca
    12061 attaaaaacc gcattcttac agttggtgag attactccga ttctattctt tggattgtca
    12121 taacaaaaac ttttaaggca aaacgtaagt tagcacgctg accgctccga aacagccata
    12181 gccgccttcg actgtgctaa gggtttccag aaaagattgc ggaattatgc tcgaagtggc
    12241 attcagtatc gtcgccatat tctcatcgta gccgtaaacc tggatcgcca gtggagcgtc
    12301 aaggttgttg ttgagtttaa attgcaattc cttgacaatt gccccgtcat tcgagaatct
    12361 attgaccagt tgaccatttt cacacaacaa ataaacatca tacagatagg tgtcggcagt
    12421 cgtgcgcaaa ttgaaattta ctccgtcttc ggaaactaca accgagccgg tatcaattgc
    12481 cggcaaatga ggaacgctgg tgctggcaga taggtcgggc aaatccgccg ccgtgatggt
    12541 caattgatag gtttcaccgg cgactgggcg gaaatcagcg ttgatgtatt tatggtcgct
    12601 ttgttcgccg taataacgga aaatgtagcc ggtggtatcg cgcgtacccg ctatccatac
    12661 gttggcattt ttgataatcg gtaaggaatc cacccaggtt tcgtattcat tggaggcata
    12721 cgcccgctcg atataaacaa aagagctcgc cgtagtatct tccagccgca caatccccaa
    12781 aatattcaag cccggaacga attccgtctc aatgatcgcg tccggcattg gcccgggttg
    12841 ccaaggcaaa tcacaacttt gccagcccgc cagaccggct atgaccgacg ccaatattat
    12901 caataagtta tctttaaata ttttcataat taaaaccgga gcgtatatcc aaaatttaca
    12961 ctgggaaaat attccaatcc gaaagcataa tagccgtatc ccggaaaata aaaatagtag
    13021 taaggattgc gccgcaaaaa taacaaattg cgaatagtca tagtcatata agcctctttc
    13081 aatccgagat attccgacag gtactgacca aatccctcgc gcagtctttt cttccagcct
    13141 atatcccaga caatgcgcgg cggaaagcgg acgttgttct tcaccggggt tatatcgcgc
    13201 gggaaaccgc ctacgctatt ttccagcggg ttataaaaat attggttata acgcccggtt
    13261 tcccaggtgc gcggataacc ggaactgaat tgaaaagtcg tgctggcggt gattgccggg
    13321 gtcaatttat acattaacaa aagtttaaga ttatgcggct gatcgccttc atagtaatac
    13381 catcgtccgt tttgcaatga tgggtaactg cgagtaccgt gagaaaaagt atagctaacc
    13441 catccggaaa ggcgctcgac ttcaccgcgc aaaagccatt cgattccgta agccttgcct
    13501 cgcccggctt cgagaatctc agacagttca agtgattttt tagcggcgtc atattccagg
    13561 cgataaagcc gagttaaatc tttgtaataa cccgtaaccg aatattccag tttatcggtt
    13621 aaaaagccct cgactccgag aatgtaatgg atagattcga ccggcttctg gttttccaga
    13681 ttgtagtaat aatccaggaa ttgaatgaat tctcgattgc gtgagtccag agtcgttaga
    13741 tactgataat aatgccccca ggcggctttg actgttgtga ttcccaatcg cagagccaga
    13801 gcggcacgtg gttcaaaatg ccaactttct gatgacattt gccgcgaact gcgcccaccg
    13861 aatttcagcg ccagattgcc gatttcgatt ttatcctgaa tgtaaaccgc ttgtaaatcg
    13921 gctccaatac gacgtgccga agtatcagtc tgcccaccaa ttttatttaa aaaacttaaa
    13981 tggctttgct cccaaccgaa cttgagagtc tgattccaga aagtaaacag tgaaaggctg
    14041 gctttggcgg tataatcttg cacggaattg cggattgaag tgcgatattt gataatccgg
    14101 tctagattgt tgactgtatc cggaaaaaca aagttaacat aggaatcatt atcagcccgc
    14161 gagccggaat ggaagaaatg agtttccagt aaaatatttg tgcggattaa ggtatgcgtc
    14221 tgaattccga ccgcccgatt ctcggtatcg gtgataagac cttcctgaaa attttcgtaa
    14281 aaatcgtaag gaaagtacaa catcaagtta tccagactgt aatcgagata atcgcgcgca
    14341 taaaaagccg ttaggcgcaa ttgggtgcgt ccgattttac tcaggtacga gccgacaaaa
    14401 tcgcccatca aattcggtga attcattagc acccgataag tcgggtcgga aatcagacgc
    14461 ccggaaagca gagcggtggc atttttcttc accggaaatt caatcgcacc gctaatccca
    14521 gaggtggatg gccagaactc accatgcact tcgctctgat gtccttcgcg ggtggtgata
    14581 ttcagaatcg aggaattgcg gccaccatat tcggcatcga acccaccgac caacatttcg
    14641 atatctttga tcgaataagg attaaaaata gcctgcgagc ttagaaagtg ctgagggttg
    14701 tagattgtca taccgttcaa ttgcaccaga ttctcccccg ggtcactacc acgcacatgc
    14761 aactgcggtg aaagcgggtc cgacatactc acactcggcg aaaattttaa taattgaaaa
    14821 acatcgtcac gtaattgcgg tgcctccaat aggacaatcg ggtccacctg gaaactggca
    14881 atatccattt ccttttcgat taaagtgcgc cgctggccga tgacctcgac ggcggaagtc
    14941 ggcaaagccc gcgccgtcat ttttatcgtt cccagaaaaa gcgccttgtc gctaacctcg
    15001 attcggagtt cacgttcggc ataactaatg tgcgagaatc ttaaagtctg aagtccagcc
    15061 ggtacctggc ggacaacgaa aaatccgcgc aaatcagagg cggttcctaa accggtgttc
    15121 gcaattagaa cattgacacc gataatcgct tcgccggttt tttcatctac aatataaccg
    15181 gagatgttgg cggcttgcag accggtgacc aaaatcaacc acaggctgaa tattttcttc
    15241 atggtagcac tagccctatg gagaacagca agccggtttc cagagtatta atcactgacg
    15301 gcaataaatt gaccgcctga tgcagaccag cacctacgaa aaatcctttg tgataaatcg
    15361 gccgatattc aaggctggca ttgaacccta aattgatcaa cataatgttc cagaaatcaa
    15421 catagtcata ttcgcgggga ttgaaattgt tgaaaccaac ccgaacagtg gtattgagac
    15481 gcgccaacca ggaacgattc aggcgaaaat accactgctg atccctgccg ccgatagcga
    15541 tggattcgtg tttcgaagat ttgcggtaat atattcccgg cgaaccgcga atgaacttta
    15601 ccatctgatc ggctggatat tccagcgcca gatgagcaat tacaatatag ccgtcgccta
    15661 aactaattat atcagtccaa gggatcatct gccgccggta acgataaggc gatactggta
    15721 aatcggaggc tggtcgtctt tgcaaagatt cggtctgggc aatcatccat ttatttagta
    15781 gaatggcgat attatctgcc ggtttttgat tcttagccga cttctcgcgc aattcccatt
    15841 gccaatcgcg cacggtctct ccactggaat ctaccagccg cgtatagccg ttcaaggtgc
    15901 gagccgcgcc gaatgctgga ctggcgtcgt accagagact gagatgatca ataaccagag
    15961 tttggttgcc cgtaacctga aatttttgga aaaacttgga catttgtatt tccagtggtt
    16021 cattcaaggc gatgtactgg tcaaccggaa tgtagtgcca tttcttgatt tgctgaatgc
    16081 cgaggattgt cggtgattcg gctcgttcat cgtttaccgg gatagctgga aatcccgtca
    16141 gcgtaagcgc atcggcatag gtgctaatat aaagcgtatc ggaaattagc tccgaccagg
    16201 aaaatttaac tgctaaggca ttcgaaaaac aaagccccaa caataaagtc aaggagatca
    16261 attttgcgat attttgatta ttcaacgtca agtaattggt tcattaaggt tggtgtaaat
    16321 tttacgacca acgccaaaaa aatgcaactt taagagcgat ggagttttta ggcgcgatta
    16381 aaagattttt cccctttttt ctgacactgc ctattccgcc tgaactgtgt gaagctgaaa
    16441 ggaagaggtt tctgccgcaa aattaatgat tactgattcg gcttaacaca agtccgcttt
    16501 ttctcatacg aacaatggca ttccgcaccg cattgtccac tatgcttttc gcccttatag
    16561 cgactaaatt tcagcgccgc catcattagg gcaaaagcta acgtcgcaca tcccaatatt
    16621 aatagaaaat aaatcagcat caactttacc tgtttttact tgaatgtcgt ccgggttcac
    16681 aattttagtt catcagaatt catcgtacaa aatattctcg ggttcaacgc ccagatcgtc
    16741 cagcattttc aatacggcgc tgaccattag aggcggcccg caaaggtaat attcgcagtc
    16801 ttccggcgcc gggtggttga tcaaataatt attcaaaacc acttcgtgga taaacccaca
    16861 atagccattc caattatctt ccggcaaagg ctcggaaagc gctacgtgcc attcaaaatt
    16921 tggaaattct ctctgcaggg cgtcaaagtc ctcctgataa aacagttcct tcagactacg
    16981 ggcgccgtac cagaaggaga ttttacgctg ggtgtgcagg cgcttcaatt gatcgaagat
    17041 atgcgaacgc atcggcgcca ttccggcacc gccgccgatg aagaccattt ctctatcggt
    17101 ttctttggcg aagaactctc cataaggccc agaaattgtc acccggtcgc ccgccttcaa
    17161 actgaataaa taggacgaca ttttacctgg aggcgcatcc ggcttttctg gcggcggcgt
    17221 ggcgattcgc acattcagca taattatgcc tttttcgtcc ggataattag cgattgaata
    17281 agctcgcaca acctcttcgt ccacaatgga aacgtagcgc cacatattac agcgatccca
    17341 ttccgattta aaaggttcct caattacaaa atccttgaaa ttgacatgat gcggtccggt
    17401 ctccacctga atgtaacccc ccgcccggaa atttatctgc tcgcccggtg gcaaatctag
    17461 aacgagttct ttgataaaag tcgccacgtt gcgattcgag cgcaccgtgc aaatccattt
    17521 gcgaatggcg aaaatttccg ttggaatcga gagccacaaa tcttccttga ccgccagttg
    17581 acaggctagc cgcacacctg cgcgggcttc ccggcgattg atatgcgcca gctccgtcgg
    17641 cagtatcgtg ccgccgcctt ttaaaacttg tactttacag agtccgcagg tgccagcggc
    17701 tccgcaggcg gacggcaaat atatttcctt ttcagccaag gtatttaata gtttgccgcc
    17761 gactggcgcg tcgaaggaat aatccggatc atcatttata ttaattttaa tcgtgccgac
    17821 tggaactaag taggacttcg ccaacagaat caagccgacc agcgccagca caataaaaat
    17881 aaaaacccca accgcaatta aaattataac cagactgctc ataaaatcgt atctactaaa
    17941 gctgaattcc ggaaaaaagc ataaaagcca tcgccattaa tccggctgtg ataaaagtaa
    18001 tgcccaaatc gcgtagaccg gatggaacgt tgctatattt caatttctcg cgaataccgg
    18061 ctaaagcgac aatcgctagt gcccagccta ttcccgaacc aatcccaaaa accacacttt
    18121 caccgaaggt gtaatcgcgt tcgaccataa aaagcgagcc agccagaatc acacaattaa
    18181 cggtaattag cggtagaaaa atcccgagtg agtggtaaag ctttggtaca aatttatcca
    18241 gcaccatttc cagaatctgg acaacggcgg cgatgacgcc gatataagtt atcaaaccga
    18301 gaaacgtcaa atctaggtca atgcccaacc agctcaacgc gccttcagcc agcaaattgt
    18361 gatagattag gttattaata gggaccgtga tcgtctgaac cacgacgacc gcaataccca
    18421 gcccgactgc cgtttccaaa cgtttggaga ccgccaaaaa ggtgcacata ccgaggaaaa
    18481 aagccaatgc catattctcg atgaaaacgg acttgatgaa caggctcaga taatgttcca
    18541 gcataattta ctcctgcttt gtttccggtt tcaaagtgtg taaaccccag ataattaacc
    18601 ctatcaggaa aaaagcgctg gcgggcaaaa ccaataatcc atttggcacg taccagccgc
    18661 cttcggtatt gacccgcaga atcgtaaagc cgagcaattn nnnnnnnnnn nnnnnnnnnn
    18721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18781 nnnnnnnnnn nnnnnnnnng gcacgtacca gccgccttcg gtattgaccc gcagaatcgt
    18841 aaagccgagc aattttccgg tgccgaatag ttcgcgaatc gaagccacaa tcaaaagaat
    18901 cagactatag cccaacccat ttcctaaccc atccaggaaa ctggctaacg gcgcattttt
    18961 taaaccgaag gcttccgtgc gccccagcag aatacagtta gtgatgatca ggccgacata
    19021 aaccgaaagt tgcttactga cgccgaaagc gaaagcctta atcaattgat cggcgacaat
    19081 caccagcgag gcgatgatcg tcatttcgac gataatgcgg atacttccag gaacctggcg
    19141 ccggatcaaa ctgaccgcca tattcgcaaa agccgtaacg aaagttaccg ccaatgccat
    19201 tacgatcgcc gtctccaatt tagaagtaac cgccagcgcc gaacaaattc ccagcacctg
    19261 aacgatcacc gggttttctt taattatcgg cgtggttaaa gtggtgagtg acttattcat
    19321 ttctcatcct cttggcttga agattgcgca aaaacggccc atagccattt tctccgagcc
    19381 agaattttac gagttgatca atgccgcgcg tggtgagcgt tgcgccggac aagccatcaa
    19441 tctggtattg ggactgcgga ctggttgggt caactttacc tttgataact tcgattttta
    19501 gctggccgga atcgtcgaaa gcgcgtttgc caacccactt ggcgcgccag agcggattgt
    19561 caacttcgcc gcccagaccg ggtgtctcac catgttcgta tatgataaaa ccggtgatag
    19621 tctgcaaatc aggaccaaaa gctatgaaac catagagcgt ggaccagaga cctttgccgt
    19681 aaaccggaag aatcacacca ccagcttgat catttccaac aacctcataa actaccatat
    19741 attttggctg acgaccaatg tgtgccaaat catcggcaga tggagttgtc cgacttaagg
    19801 ccgggtcacg agcaatggct ttcgaatcaa agcgtgccgg atcgaggcgc ttatccgacg
    19861 gagtttcgct aatgatttga ccggtggcaa gctcaaccag tagcgggcga attttctcct
    19921 gaaaagtctt tttcacggcg gcgtcatcgg tgtaaaggtt ggcggcttgg agaatatttt
    19981 tcaacttgtc caattcttgg tttacttgct ggcgcggacg caggagaacc acagaagtcg
    20041 ccaccatcac cgcgcaaatt aggcaaacgc ccagtgctac taagaacgtt ttccgcgcgc
    20101 tttcatctga catcgcgggt tgctctcctc tttatatgag cgttaataaa aatgcgatct
    20161 attagcggcg cgaacatatt tccgaataaa atcgccagca tcataccttc cggaaaagcc
    20221 ggattcaaaa cgcgcaccat aataaccata acgccaatca gtaaaccata tacccattgg
    20281 cccggtttgg taactgcgcc cgaaaccgga tcagtcgcca taaaaactgt cccaaaagca
    20341 aatccgccca gcaccagatg ccagagcggc gtcaccgcga acattggatt agtggcactg
    20401 cccattaaat tgaaagttgt agaaagtgtc accattccga ttaaaatact cagcataatg
    20461 cgccaactac ctactccggt aaccaataaa attagcgccc caattaaaca ggctaaagtc
    20521 gaagtttcgc ccattgaacc gggaataaac cccaggaaag catccagcca gctggtagta
    20581 actgccgatg acgtttccgc gaactgcgcc agcggcgtcg cccggctaat gccgtccact
    20641 gcgatccaaa ctttgtcacc ggtaatctgc gccggataag caaaaaacag aaaagcacgc
    20701 gccaccaatg ccgggttgaa aacattgaag ccgacgccgc cgaaaacttc cttgccgagc
    20761 actacaccga atgaaattcc caatgctacc tgccagtagg gaatcgtcgg cggcaggatc
    20821 agcggaaaaa gcaggctggt aacgaaaaaa ccttccgcga cttcatgttt gcgaattacg
    20881 gcgaacagca cttcccagaa tccgccaact atcagtgtta tgatataaac tggcaggaaa
    20941 tataaaccgc catggatgaa attggaccag aaactttccg gattgaagtc cagtcccaat
    21001 gccagcatca ggcgttgttg ccaggtaaaa ttagcgccgg agccgagtga ctgcaatacg
    21061 agatttgcct gcaagccagt attataaaaa gccatgaaaa ccgtcggtat cagcgccacg
    21121 aataccgtga tcatcatacg tttcaaatcc ataaaatcac gtacgtgcac cgtgcctttg
    21181 gtaattttac ccggagtata gaaaaaagcg tcaatcgctt cgaataggta atataatccg
    21241 tctagcttcc cgccgggtcg aaaaggcttg cctgcctgat cgagaaattt ccttagtccg
    21301 cccattaacc ttctttctcg attaaattca acacgcgccg cagattctga ccatggtcaa
    21361 ttttcgaggg acagacaaat gtacataacg ccaaatcttc ttcgaccagt tctaaacaac
    21421 cgagtttttc ggcttcttcg aggtcatcta ccgccaacgc tcgcagaagg taggtcggca
    21481 aaatgtccaa tggcattacc ttttcgtaac taccgatggg aacgatcgcg cgttcactgc
    21541 cgtgcgtatc ggtgtcgaag accagcttac ggtttggtaa aaaagccgcc agcgcaatat
    21601 tcttcactga aaatagccgc gctcccagac taagccaacc aaataattta cgacgtccac
    21661 cttctgggat gacacttact atttgatgaa accgtccgag aaaattcaac tcgcctgccg
    21721 ctgtatgtcc ggaaagaacc gagccggaaa ttatgcgatt atggccttct gccagttcgc
    21781 cgtcggttaa ttcgcttagg tttgcgccga ctcgcgtggt aatcagtcga ggctttttaa
    21841 ccgctgaacc cgccaaagcg acgattcgcg cggttggcaa tctgccggta gtaaacaatt
    21901 tcccgatagc cgcgacatcc tgggcatcaa tgctccaaac ggttttnnnn nnnnnnnnnn
    21961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22021 nnnnnnnnnn nnnnnnnnnn nnnnnntaaa caatttcccg atagccgcga catcctgggc
    22081 atcaatgctc caaacggttt tatggagtga aaccgggtcg aggaaatgaa tatgcgttcc
    22141 taccagtccg gcgggatgcg gtccggcaaa ttccactatt tctatgtttt caatttcagg
    22201 aatgggaatc gctgtatccg gtgcttggca gacgaatact tttccagcag tcagtttggc
    22261 aatgagcgct aagccgcgct ggaaatcatt cgcctgttca cgtagtatga ccgcgacgtc
    22321 tggcgccaga ggattagtgt ccatagccgt gacgaaaatc gaatgcggtt gactttccgg
    22381 attagctaat ttaccaaatg gtctggcttt gaacgagggc cataaaccgg actccaacaa
    22441 ttgatttgta atttcttctc gtgtcaggga accaatttgt tgctctgaaa acgatttgaa
    22501 ttcaacagca tcatcgccat ccaatttgat taccagcgat agaaaagctc gtttggcgcc
    22561 gcgattgatg gcaatgacct gaccagtacc tggagcagtg aatcgaatct gcgggtaagt
    22621 tttatcgcta aaaaggggtt gcccgagttt cacctgatcg ccgatcgtta ccgcgaattg
    22681 cggcttcagc ccgacgtaat ccagtcctaa aagcgctacc tgcgagaccg attgagccgg
    22741 gtaaataacc tgctccggtg ccccggcaat tggcagattt aatccctttt taattttaat
    22801 taacattagc ggtacgcttt ttttcccgat acgattggta taattaaatg tctgttgtat
    22861 ttatactgaa ctaaatctta gtcgaatttt cataagtcgt agattaaaaa aaattcatat
    22921 attgaacaaa cacaaaaatt acttttttgt agtaaaattt aaactttagc agaatgtctt
    22981 gaattttata gagaatgagc tgggtgcttg ttggcaaaga agtgctgtta agttctattg
    23041 ttttaatggg atggcaaatc gccgttaatc tcttataagc acttaggaga gcttaatatc
    23101 ttggttttcc tggcggttga ttattttgct tagttggaag cggggacgca ggaattcggt
    23161 gaattgtaac cggcaactgg aaatcgtggc tcaccccgga aagttcatat tctcggcaaa
    23221 tatttccata aattgagcgt cggtagctag tggaaagtgt tttgtaataa gcggaatcga
    23281 gtggtactct tgctcattaa gtaaagacaa cttggcgccg tgcagagcgg tatttccggc
    23341 ttgttcaatt aattctgtgg gaaaatcaaa cagaccgatt cgccgcgcgc tgacgacatt
    23401 gatgtaattg ccgaatgcac ctgccagaaa aacttgcttt atatcagccg gtttcaattg
    23461 catttgtttc cgtaaaatct ggattccagc cgcgatggcg cctttagcca attgtaattc
    23521 gcgaatatcg gcttgagtaa cgaccaccgg cgtttggagg ggaatttctc gctcgggtaa
    23581 ggtaattcgt ccggttggat caatccagtc gagatcgagg ctagcggcaa ccgcgtccac
    23641 tagtccactt ccgcaaatcc cgcgcgccgg gacattgccg attacttcaa cttgcaactg
    23701 gccattggta agccaaactt tccagatggc gccggtggtg gcgcgcatac ccatcgtaat
    23761 ctgagcacct tcaaacgctg gccctgccgc agtcgaagca cacagaattc tgtcacgatt
    23821 accaatgaca atttcgccgt tggtaccgag atcaatcaga gcgacggttt cgggactgcg
    23881 gtgcattcca accgccagaa ttccagctag aatgtcactc cccacgaatc cgccgagaca
    23941 aggtagaaat tttactttgg gattgcccgg caactgccag ctgatttcct ctgccgacca
    24001 ttccaattca ccgggttgct cgatttcaaa cggcaatagc gccagcggtt caaccgataa
    24061 cccgccaaac aagtggtgca taacggaatt accgacgata ttaatctgtt taagtcgccg
    24121 ctccggacag cgacattttt ctaccaagtt agcaatcatc tgtccgattt gatgacgaat
    24181 caggttttgt aataattctc ggcctgcggt ggtcaagccg aactggatgc gactcataat
    24241 atcggcgccg tggcgcgctt gcggattgag tgccgtttcc acgcccagaa ctattccgct
    24301 ggataaatca agcaattggg ctaccagagt agtcgttccg agatctaccg ccaccgccaa
    24361 gctgtcttct ccggcgggtt ttatccgggt agcatcggcc agaattgaga tttccagctg
    24421 ggcgagctcg ataactaatg gttccgtcac ctgacattga caggctaaac gccagccctg
    24481 gc
//
LOCUS       DAOSMP010000034        24089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_2111_length_24089_cov_19.647749, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000034 DAOSMP010000000
VERSION     DAOSMP010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 24089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24089
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2111_length_24089_cov_19.647749"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..2813
                     /locus_tag="PKX09_06890"
     CDS             117..2813
                     /locus_tag="PKX09_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU17582.1"
                     /translation="MNTKKRPYEDIAEELVCESEDKLASLVRNLPGAAYRCLYDAERT
                     MIFISDGCRSLTGYSPDDFLMDHKITYSQIIHPNDRQYVFDEIGASLKKRSPYQLLYR
                     IMTADNQEKWVCEQGVGIYNGDENLVVLEGFITDISEKVKANIALQSYETRFQSIWEG
                     SFDGMRLTDKDGKIIAVNKAFCRQVGMRRNELENASIGIIYPPEERQEIETNYRNNFS
                     RRHIKSLTECESILWNGQKVWFEISNSFLDIPGEQVYLLSIFRDITKRKNAEAELAES
                     ARRFRWLYEYAPIAYHTITPDGIIVDVNRQWCEIMGYSKEEVCGRPIFDFVIESERES
                     ARKSFDEKKLSKKTSSTGNERHYLTKSGEVKTFSISDYIIRDDDGRIRSIQTTMSDIS
                     ELKRTEEALRMSEAKFRHLVESSLDVIWQADDSGRVLYVSPNIKDILGRTPDKLIGKQ
                     FADILPKGSEEKGGKIIASHFTKRKPIKNLELTIVNHLGQEIILEVNGVPNYDGGKKF
                     TGYIGTMRNVTIQKIVEDEQIKVQKLESLSILAGGIAHDFNNMLSGILGNISLAKMKV
                     KDSDITRILERSEKAALRSRSLTQQLLTFAKGGAPVMESIQVESFLRELVEFATVGSS
                     VNCQFDIAPDLPNVEADRGQLSQVIQNLVINAIQAMPSGGNLKISANAEEIKTDGLVP
                     IKSGYYLKIAIQDTGVGIPKNLLSKVFDPYFTTKPHGNGLGLSVVYSILKKHDAYIFV
                     DSEAGNGTTFTIYLPATAAVQKGSQKAEPQISKGNGRILVMDDDELICEVVEEMISSM
                     GYDVKCVPNGEEAIKSYKEAKEAGQPFDLVIMDLTIVGGMGGKETIAELKKFDPDVKA
                     LVSSGYINDSMMTVPQKYGFIDIIAKPYRMEDLSSILKEHVG"
     gene            2957..5596
                     /locus_tag="PKX09_06895"
     CDS             2957..5596
                     /locus_tag="PKX09_06895"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOU17583.1"
                     /translation="MKKLVIIVKLSILAIFISGTAMGNTQVKATRTDESIRIDGILSE
                     EIWQRAIPAPPLIQDEPNEGAQPSQPSDIWIVYDDEALYVGARLYDSAPDSIVARLGR
                     RDSDWNTDLFIIALSPFNDKRTAYYFGISAAGAIYDGRFYNDNWQDDNWDGVWEGAAQ
                     RNQEGWTAEIRIPFSQLRFQKSDQYVWGVALYRKIQRYNEEDMSFVSPRNESGMVSRF
                     IELVGIENINAERKIEVLPYIRGKGEYLAVDPADPFNDGSRYFASTGADFKIGVGNNL
                     TVDGTINPDFGQVEVDPAVINLSDVETYYNEKRPFFIEGSDIFNFGYGGSNNYWNMNF
                     STPSIFYSRRIGRAPQRDLSDYDYTDVPDGTRIISAGKLTGKVGDNWNVGLLNALTAR
                     EFARVSPNGLDSKIEAEPLTNYAILRTQKEFQEGRHGLGIITTSTKRFFRDESLADYL
                     NSDALVVGLDGWTFLDTNKTWVIAGWGAMSNIQGNENCITDVQTNARHYFQRPDAEHL
                     GVDSNATALTGFATRINLNKQRGNVIFNAAFGLIDPRFEVSDLGYLSRGDVINYHVGA
                     GYQWTEPNHFYRKFNLILAHTLGMDCGGNLTNNGIMSFSYVQFSNYYWIDYGGGYFWE
                     TYDTRLTRGGPIAISPSGRWGYIQISTDERKPLVGRLYYETSGNDTASVSHYLQIPLN
                     WKVADNLAFELTPSISVNHNYAQYVDVFDDPYADATYGKRYVFAKMDQKTISASLRLD
                     WTFTPRLTLQLYAQPLYGAAEYYDFKEFARPKTYQFFTYEEGTEINRNSDGNYTVDPD
                     GNGPAGSFSFDNPDFNVYSFRSSAVLRWEFALGSAIYLVWTHGRFENSSEADFDLSRS
                     VNRIVEIKPDNIFMIKASYWFNV"
     gene            6153..6284
                     /locus_tag="PKX09_06900"
     CDS             6153..6284
                     /locus_tag="PKX09_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17584.1"
                     /translation="MTTESENGNPKEREAELIQKIRNRVAEVQELVDEMKSRLTDKK"
     gene            complement(6575..9178)
                     /locus_tag="PKX09_06905"
     CDS             complement(6575..9178)
                     /locus_tag="PKX09_06905"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU17585.1"
                     /translation="MPDTDSDVKNLTILSDTNPRLLTDGTLYTDLQLSPPEEDYLKLV
                     TTETINDSVGMATKSDQLGSIVNNTGNLPPYPPYDPSPTHQQIRQPDGYLEWMMGEDP
                     DGDEIFYCEIIIRNELDPDQMITVHLNDSILEKAREGGVEGLSYEYNGQVRFGLKRIQ
                     DEDFFRDGWVYSWSVRVSDKWGGTTESNWTEATFRFDDGINQPPLPPVEGFNPDGIIV
                     ASHYPELKWSPAIDPDVADRLRYEIVLSRDRSFGGRTYILLKSTYDQNQILVQTPLLE
                     NCQYFWRVRSIDLTEARSVWSKSNTFWVNQINEAPQRPVRIIHPHNLEEITPNSYFWW
                     LPSDDPDPGDQLNYQIECSESLNFTEPIFSYRIPRTITAKEVETKLELPQRAIGVCLD
                     EISAISLLKDNTLYYWRIRAFDQSEISGTSASRPVRVAFNHQNDPPLPVVKGFYPADD
                     AIIKTLRPEIRWANSSDPDFSDFQASLTYQLEISRDPQFPENACSVYTTEPGQTVFTL
                     PENLIENERWFYRVRACDQHGSYSLWSPTNSFITNAISEAPYPVTDGFLPKDSAVVET
                     AQPLISWLPSDDPDPDQDERDLYYLVRFYEADNPKKVSQVASKTGITSLHLPELKEDK
                     YYFYQISAVDPDGNRSEWSQLVCFGVNAVDLPPGPFALLAPFNGQDSVALDAGFVWHI
                     AHDKDPGSQINYTLYYGTDSLFLTNFREIMFEQPEEDTIMVYNPPERLSYATRYFWKV
                     VATDNRGNQCWASQTNKRPFVFTTVGTQRSIASIFKNFRLHQNYPNPFNIETLIRYDV
                     PFYGPVDVSIYDLIGMKVTTLATGNHAAGSYSVYWNGTDQNGTLVANGVYICRLTALG
                     YMSHIKVVLMR"
     gene            complement(9487..10785)
                     /locus_tag="PKX09_06910"
     CDS             complement(9487..10785)
                     /locus_tag="PKX09_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018887199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOU17586.1"
                     /translation="MPKNQITNLPHNQIDTTVFQKVYGHNPSVITEQIKRYAYLADRH
                     NEIFATSPALFFSAPGRTEISGNHTDHNHGKVLAASVDLDSIAAVNAINGNQITIFSE
                     GYPQPFIVDINHCSPVEAERGTTTALVRGIVARFKELGLRVGGFNATVTSNVLVGSGL
                     SSSASIEVLLGTIMNYLFNDGQVAAEDIALIGQFAENQYFGKPCGLMDQLAIAVGSVV
                     AIDFKNPSKPIIQKVNYDPEQQNYSWLIVNTGTSHVNLTEEYAAIPREMRAVAKALGG
                     QVCRDITMQNLIEEIPDLRPKVGDRAILRAFHFLTENERVQQQVSALERGDFATFLEL
                     VNESGASSCQWLQNCYPTSNPAEQGVPLALALTQNFLKTAGKKGACRIQGGGFAGTIL
                     AFIPKEILSDYLRLITKIFTEGSVSQLNIRPIGTVCLNKV"
     gene            complement(10795..11844)
                     /locus_tag="PKX09_06915"
     CDS             complement(10795..11844)
                     /locus_tag="PKX09_06915"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HOU17587.1"
                     /translation="MSFDFSQIPHRRFNPLTGEWILVSPHRAERPWHGSQEPVVSEEM
                     PEYDPECYLCPGNRRASGTVNPLYTSTYVFTNDFPALFPDTPHRRRNVRKYIRDESVS
                     GTCRVICFSPRHDLTLAEMAVEEIRNVITVWAEQTKELGENFSWVQIFENKGAMMGCS
                     NPHPHGQIWAVNSLPNEPYKENRRQSAYFKQNKSVLLTDYLAYELESRERVVLENENW
                     LAVVPFWAIWPFEILLLPRRPILRLPDLTPAERNSLALILKSLLTKYDNLFQSSFPYT
                     MGWHGAPTMPGNFDYWQLHAHFYPPLLRSSSVKKFLVGYEMLAEAQRDLTPEVAALTL
                     RETSEIHYKSLNKDV"
     gene            complement(11877..13484)
                     /locus_tag="PKX09_06920"
     CDS             complement(11877..13484)
                     /locus_tag="PKX09_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HOU17588.1"
                     /translation="MRQVVFSWYDGLIFMLFFLFVVIFSMYKSRKGKTGEDYFLAGRQ
                     LTWPLIGLSMIAANISTEQFVGMAGQAAGSAGLAIASYEWMAAITMVIVALFLLPSFL
                     RTGIYTFPEFLEYRYSPLARHLMAVYTILIYVFVLVASVIYTGGLTIATLFSGMKFFG
                     IEVTLTRAIWLIGIITAIYTIWGGLKGIAWADLFFGSSLILGGFLTLVIGLRHVGGLS
                     GFLEANQDKLHMILPRDNPVLPWTALVIGLWLPNFYYWSVNQFITQRTLAAKDLREGQ
                     LGVIFASFLKLITPFIIVFPGIMAWQLFKDQMTGPGGTDAAFPTIVRNLIPNGFLGFI
                     FAAIGGAVISSLASMLNSASTIFTMDIYQHAVKKELPQQKIVRVGRISTFVFLIIGCL
                     IAPILDNPSLKGIFTYLQEFQGFISPGILAAFAFGLIFKRAPKAAGLAALILNPIIYG
                     LLLIFCGSLPVFASVQIVPIAFLNRMAITFIIIVVIMGIITWLKPLDQPVTMPERKDF
                     NMQSSPIVKWLGAMVIGLVIVLYIIFW"
     gene            complement(13847..14434)
                     /gene="coaE"
                     /locus_tag="PKX09_06925"
     CDS             complement(13847..14434)
                     /gene="coaE"
                     /locus_tag="PKX09_06925"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOU17589.1"
                     /translation="MVTLGVTGGLGSGKSTACKFLAEKGAYVLDADLIAKQLMENDPQ
                     VRDEIIAAFGNDVYKDGKLDTQKLAHRAFASEADQRILNDIVHPRVIDYFQQEVDKMR
                     DKYDLIVLDAPLIFESGFDSRLDHTLLIYTKYKYRLERALRRGNLTREDILRRIELQM
                     PEEDKRELAEFVIENNGTEEQLKSAIGELYDKLTT"
     gene            complement(14441..15085)
                     /locus_tag="PKX09_06930"
     CDS             complement(14441..15085)
                     /locus_tag="PKX09_06930"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOU17590.1"
                     /translation="MKVILLGPPGVGKGTQAQKLQQERGLRQISTGDILRQAIKNQTE
                     LGKQAEDYIKRGELVPDDLMLGLIAETLFGDDAPQDYILDGFPRTIPQAEGLDRLFAQ
                     HNVDLDAVILLEAEPEAIIKRLSARRTCRQCNAVYNLITNPPKNPLRCDICGGELYQR
                     DDDRIETIQRRLKVYTKQTEPLAEYYRQRGKLKTIAADGTPDEVFQRINRVIGG"
     gene            complement(15142..16776)
                     /locus_tag="PKX09_06935"
     CDS             complement(15142..16776)
                     /locus_tag="PKX09_06935"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HOU17591.1"
                     /translation="MKYIGIIGLLVILGIAFLMSNNRRLISLRVVIWGLLLQLLFAGI
                     ILGEMLISYFTLFIFLLLIIIFIYKDQINTVGHRSKQWLYIGLISIATVLATAAFYWF
                     GKTGWLAELIGLITVAIVIKAIFKVRPWQKELTVAWLTAGLSWLILHGYTGQDIFAFL
                     SQKVEKFLGLATLGAEFLFGNLALDKYFFPNETTWPGFGMQFAFTVLPVIIFFASFMA
                     ILYHLGVMQAIIKAMARFMQWTMGTSGAETLSCTANIFVGQTEAPLLIRPYVDQLTLS
                     ELLTIMVGGFATIAGGVLAGYIKMGVPAGHLIAASVMSAPAALVIAKIIYPETDHPQT
                     AGDVDLPEDTVKSANLLDAAARGVTDGLKLAATVGAMLIAFIALIGLVDTILGFIDRL
                     IDGNLLKGAYTIYTNSSGFSPVKGEFSGIFPGSLKSLFSFILRPLAWLMGVSWQYAGE
                     VGNLMGIKISLNEFVAYSALSELIHTGALDQKSIVIATYALCGFANFASIGIQIGGIS
                     SLAPGRRSDLSKVALKAMFGGALASWMTATIAGLLL"
     gene            complement(16820..17440)
                     /locus_tag="PKX09_06940"
     CDS             complement(16820..17440)
                     /locus_tag="PKX09_06940"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOU17592.1"
                     /translation="MSQPNLHRVPTDFEEVVTVTEKSAGWIEVICGSMFSGKTEELIR
                     RLRRAQIARQRVAIFKPVIDVRYSEDYIVSHNQQKIPSIPIHTAQEILQKAGNAQVLG
                     IDEAQFLDNELVAVCRTLANSGRRVIVAGLDKDYRGEPFEPIPQLLCEAEYITKTLAI
                     CMKCGNPANFTQRIIKSSERVLLGATDIYEARCRACFEPPIIEEKK"
     gene            complement(17452..18090)
                     /gene="udk"
                     /locus_tag="PKX09_06945"
     CDS             complement(17452..18090)
                     /gene="udk"
                     /locus_tag="PKX09_06945"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOU17593.1"
                     /translation="MKKAILIGVAGGTGSGKTSMARNIVHDFDPRDVVIIEQDSYYYD
                     LSDIPLESRGHHNFDHPDAYDFPQLRADMSAILNGQQVKIPIYDYKTHTRSKTESQVV
                     NGQKIIVLEGIMVLYDPELRDMMDIKVYIETAPDIRFIRRLRRDIHQRGRTVDSVIAQ
                     YLETVRPMHEQFVEPTKHFADIIIPEGGQNSVAIDLFKTKVRSLLRHLNEPR"
     gene            complement(18091..18498)
                     /gene="cdd"
                     /locus_tag="PKX09_06950"
     CDS             complement(18091..18498)
                     /gene="cdd"
                     /locus_tag="PKX09_06950"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOU17594.1"
                     /translation="MEKIKPELLKVAARQAANNAYAPYSHFPVGAALLTNDGKIYTGC
                     NVEASSYGLTVCAERNAIAAAIVAGERYFRAMGIFSLTRVSPCGACRQVIWDICGDID
                     IFLFDESEDYQHFRSSELFPIPFDNNQMKGIDK"
     gene            complement(18471..19163)
                     /locus_tag="PKX09_06955"
     CDS             complement(18471..19163)
                     /locus_tag="PKX09_06955"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOU17595.1"
                     /translation="MFIPLRDSTMRLTPPYVTVGLITLNCLVYIYQLTLPPEILVAFL
                     NTYGLVSANLFSHPSLRDFLSFFTTLFLHAGFLHLFGNMLYLWIFGDNIESTLGHGRF
                     LLFYILCGVIAGLTQVLITPRSTIPMIGASGAISGVLGAYLLRYPHARVKVLFWFFIF
                     IRQIWIPAGYLLGFWFIVQLSNGLGSLGFQTQGGVAWFAHIGGFLAGIILLLILEPYE
                     RKRTWKKLNQNY"
     gene            complement(19172..21415)
                     /locus_tag="PKX09_06960"
     CDS             complement(19172..21415)
                     /locus_tag="PKX09_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOU17596.1"
                     /translation="MRYFPLASVIVLLFQTTLSQNYLWPTNSSRLISSTFGEYRVNHL
                     HAGLDIKTNQRTGYPVFAIENGYIKTIRTSFTGYGKVIYHQLDDGNIAVYAHLDDFAE
                     PLSTIVKAEQERQKRYRIEVNLKQDDLRVTKGQIIGYTGETGTLHPHLHFELRDSLDN
                     PFNPLNTNLTVNDKTAPTISGIAVTPISLDARINGLPMTQTFKTIQKKINHHILKKPI
                     QVEGQFGIEIKVHDTIKGVSNVYPPYGIKLFIDDSLYFQVQYDRFSYEQTHFAIIDRN
                     YQLDHDLGEVYNRLWTIAPHKNMPMNTLPEATGIIDLETGLHSVRIIAYDKNQNQAMV
                     DFKILSAPVTSFESVEIQTGEEGYQLFLSRPAQPIVENFKARWVNKNGHYLRQANLAL
                     TDTTAEFYKLSLIDQPVDNEYLKIEAVGVDGNQFQPLFINLNQAEKSSVISLNYTFIH
                     NPKNFLMLLTFSAVPDSNPTFDLQTINGLGKVELIRISPTEYMTVPVPFAYWRDASAY
                     EIRFDNQLTNILRGRLNLKCIVPSNENFCLSQDSVFKAVFPRDAVFDSLLVWLETKEA
                     TEIKGGKMISARYGLHPTNQPLHDSIQVCFKNPVCAQDISQVGIYQYVDKNWQFRGNQ
                     LDVNQDFIWAYSRQTGEFALIKDYTPPVIHNIFPGNGGRFHANDVHYLKATVHDQLSG
                     ITDDQSIVVTLDGQPIIAEYNAPKNQIRYRLSRQLTAGKHALSITVTDRAGNVKTAVS
                     TFTIIPG"
     gene            complement(21509..22330)
                     /locus_tag="PKX09_06965"
     CDS             complement(21509..22330)
                     /locus_tag="PKX09_06965"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOU17597.1"
                     /translation="MKDWDQNLFEEFVSQIEAVSRGEYNDESFKKFMRIGVPNSLRQL
                     ATILDNLLRRFETRELHLKEAIEELKSARSALQDLNTLLDSRVRERTQALEEANRRLE
                     ALSTTDALTGIYNRRYFDQELKRETARSTRYNSPLSLLMLDIDLFKIVNDTHGHLFGD
                     EVLKTLGQILLTCLRKNDFCARYGGEEFAVLLPETGEKEAFLVAEKIRTIVAKRKIYF
                     EQAVVSITISIGIAQFNPAGMIDETQLVDAADKALYQAKRSGRNKSVIFDYSGLA"
     gene            complement(22345..23010)
                     /locus_tag="PKX09_06970"
     CDS             complement(22345..23010)
                     /locus_tag="PKX09_06970"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOU17598.1"
                     /translation="MNNFSPDNCQTADKMVAEQILARNIKDPELLEAFRQVPRHLFVD
                     DTQRTLAYSDQPLPIGYGQTISQPYVVAFMIAALQIDKSAWVLEIGTGSGYETAILAR
                     LAGNVFTIERIGELSQRAQKVLSQIGIHNVEFRIGNGCLGWPEKAPFSHIIVTAASPE
                     IPPALVDQLALGGRLVIPLGSSCGGQELTLIAKTPLGLNSQKILPVRFVPLIWDSSNT
                     NHS"
     gene            complement(23007..23825)
                     /locus_tag="PKX09_06975"
     CDS             complement(23007..23825)
                     /locus_tag="PKX09_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17599.1"
                     /translation="MYKNCDPQQMPLFPDLFEDIYLPPITTLPEFNQEIDNFIKLADF
                     GALIDLTFQGLDKSYSLRLSDFQIPPQMIRDDARPESPIFNLFPPEHHRRLQRFRYEI
                     KAFFNNQNSIKTPNGYFLLRSHFQQWENYRGQFYQKIVNYLQNDIGPRRYRNFFETCF
                     KSGLEWLNAQLRPIHPYQVQFTDLQEISRRRKSLQSTRTTLAQLAPEDPEYLLNCITL
                     KTLHIPLTLVQFIKGVAINSTFKTIHLNYLKDVKIETLRDVKHLLESISRQSDQ"
     gene            complement(23838..>24089)
                     /gene="glgC"
                     /locus_tag="PKX09_06980"
     CDS             complement(23838..>24089)
                     /gene="glgC"
                     /locus_tag="PKX09_06980"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257634.1"
                     /note="catalyzes the formation of ADP-glucose and
                     diphosphate from ATP and alpha-D-glucose 1-phosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOU17600.1"
                     /translation="IEKCVIMGADYFEQVQPEGSPQLGIGQNCVIKNAIIDKNARIGN
                     NVQIVNRDQLKEYDSELYSIRDGIVVIPKNTTIPEGMII"
BASE COUNT         6863 a         5098 c         5521 g         6607 t
ORIGIN      
        1 ttagtattca cacgtggaaa acggcacaca ataaaaaaat atttagttct tgggcaacat
       61 tgtgtaggcc ggttattttc gaatagtagc tgttctgatt aatttgaggg gtttaaatga
      121 atacgaagaa acgtccttat gaagatatag cagaagaact tgtttgtgaa tcggaagata
      181 aattggcttc tttggtaaga aatttaccag gtgcggctta tcgttgttta tatgacgccg
      241 aaaggacaat gattttcatc agcgatggtt gccgttcatt aaccggttat tcgcctgatg
      301 attttttaat ggaccataaa ataacctata gccagattat tcacccgaat gatcggcaat
      361 atgtatttga tgaaattggt gcttctctga aaaagcggag tccttatcag ttactatatc
      421 ggattatgac agccgataat caggaaaagt gggtttgtga gcaaggtgtc ggtatctata
      481 acggagacga aaatctcgta gtgctcgaag gttttataac ggatataagt gaaaaagtta
      541 aagccaatat tgccctgcaa agttacgaaa cgcgtttcca atcaatctgg gaaggcagtt
      601 ttgatggcat gcggttgacg gacaaggacg gcaaaataat cgccgttaac aaagcctttt
      661 gtcgccaggt aggcatgcgc cgaaacgagc tggaaaatgc cagtataggg attatttatc
      721 cgccggaaga gcgccaggag attgaaacaa attaccgaaa taatttctct cgacgacata
      781 ttaaatcctt aacagaatgc gaatcaattc tctggaatgg tcagaaagtc tggtttgaaa
      841 tttccaattc ttttctggat attcccggtg aacaagttta tttactgagc atttttcgtg
      901 atattaccaa gcgtaaaaat gccgaggccg agctggctga atctgctcgc cgatttcgct
      961 ggttatatga atatgcaccc atagcttacc acaccattac tcctgatggt attattgtgg
     1021 atgtcaaccg tcaatggtgt gaaattatgg gttactccaa agaggaggtt tgtgggcgac
     1081 cgatctttga tttcgttatc gaaagtgaaa gagaatcggc tcgtaaatcc tttgatgaaa
     1141 agaaattaag caagaagacg agttctacgg ggaatgaacg tcattattta actaagagtg
     1201 gagaagtaaa gactttctct atttctgatt atatcatcag ggacgatgat ggtcgtattc
     1261 gttccattca gaccactatg tccgacatta gtgagcttaa acgcaccgaa gaggcgcttc
     1321 ggatgagcga ggctaaattt cgtcatttag tagaatccag tctggatgtt atctggcaag
     1381 ctgacgattc cggacgggtc ttatatgtca gcccgaatat aaaagatatt ctcgggcgta
     1441 cccctgataa acttattggt aaacagtttg ctgatattct acctaaaggt tctgaagaaa
     1501 aaggtggcaa aatcattgcg tcacatttta ccaagcgtaa gccgatcaag aacctggagt
     1561 taactatagt taatcacctc ggtcaagaaa ttatccttga agtaaatggc gtacccaatt
     1621 atgatggcgg taagaaattt acaggatata tcggtacaat gcgcaatgta actattcaaa
     1681 aaattgtcga agacgagcag atcaaggtgc agaagttgga atctctgagt atcctggctg
     1741 gtggtattgc ccatgatttt aataacatgc tttccggtat tttgggtaac atatcactgg
     1801 caaagatgaa agttaaagac agtgatataa ctcggatcct cgaaagatcc gagaaggccg
     1861 ctttacgttc tcgctccctg actcagcaat tgttaacttt tgccaagggc ggtgcgccgg
     1921 ttatggaatc catccaggtt gagagttttc tgagagaatt ggttgaattt gccacggtgg
     1981 gatcgtcggt taactgccag tttgacattg ctcccgattt gcccaatgtg gaggctgatc
     2041 gtggccagct gagccaagtg attcaaaatt tggttataaa tgctattcag gctatgccgt
     2101 ccggaggcaa cctaaaaatt tcggctaatg ctgaggaaat aaaaacagat ggtcttgtac
     2161 caattaaatc cgggtattac ttgaaaattg cgatacaaga tactggggtt ggtattccta
     2221 aaaacctttt atccaaagta tttgatcctt actttaccac taaacctcac ggaaacggtt
     2281 tgggcttgtc ggttgtttat tctatactga aaaaacacga tgcatatatt tttgtagact
     2341 ctgaagcggg caacggtaca acctttacga tttatcttcc agcaaccgcc gctgttcaaa
     2401 agggttctca aaaagccgaa ccccaaattt caaaagggaa tggtcgaatt ttagtgatgg
     2461 atgatgatga attgatttgt gaagtggttg aggagatgat ttccagtatg ggctacgatg
     2521 tcaaatgcgt tccgaacggt gaagaagcca tcaaatctta taaggaagcc aaggaagccg
     2581 ggcaaccatt tgatctggtc attatggatt tgactattgt cggaggtatg ggtggtaaag
     2641 agaccatcgc agaactaaag aaatttgatc ctgatgttaa agctttagtc tctagtggtt
     2701 atataaacga ttcgatgatg actgttcctc aaaaatatgg atttattgat ataatcgcta
     2761 aaccatatcg tatggaagat ctcagtagca ttttaaagga gcatgtcggc tgagaacttt
     2821 caaccgactt ttgattctaa tcacaaatta ttgtctactg ggttgaaaat aactcaggga
     2881 taggacaact aaatcctggt taatccaatc tatagggctt tgatgtgttt gctaatgatt
     2941 aactaggagt gtaattgtga aaaagttagt aataattgtt aagctgagta ttctagcaat
     3001 atttatatcg ggaacagcga tgggtaatac tcaggtaaag gcgactcgaa cggacgaatc
     3061 tattcgtatt gacggaattt taagtgagga aatctggcaa agagctattc cggcgccacc
     3121 tttaattcaa gatgaaccga atgaaggtgc tcaaccaagc cagccatcgg atatctggat
     3181 tgtctatgat gacgaggcgc tctatgtcgg cgcccggctc tatgattcag ccccagattc
     3241 aattgtagcg cggcttggcc gacgcgattc cgactggaat actgatctgt ttataattgc
     3301 tttgagtcca tttaatgaca aacgcactgc atattatttc gggatttcag ccgcaggtgc
     3361 aatttatgac ggaagatttt ataacgacaa ctggcaagat gataactggg acggtgtttg
     3421 ggaaggcgcc gcgcaacgta atcaagaggg ctggactgcc gaaatacgca tccctttttc
     3481 acaattgcgt ttccagaaaa gcgatcaata tgtctgggga gtggcgttat atcgtaaaat
     3541 tcaacgctac aatgaggagg atatgagctt tgtctcaccc cgcaatgaat caggtatggt
     3601 atcgcgcttc attgaactgg tgggtattga gaacattaat gcggaacgca aaatcgaagt
     3661 tttgccatat atcagaggca aaggcgaata cttggcagtt gatccagccg atccttttaa
     3721 cgacggttca cgctattttg ccagtaccgg tgccgatttt aagattgggg tgggtaataa
     3781 tctgaccgtt gacggtacca ttaaccctga ttttggtcaa gtggaagttg atccagcagt
     3841 gattaatctg tccgacgtcg aaacctatta taatgaaaag cgcccgttct ttatcgaagg
     3901 atcggatatt tttaatttcg gttatggtgg ttccaataat tattggaata tgaatttttc
     3961 gacgcccagc atattttata gccggcgcat cggacgcgcc ccccagcgcg acctgtcgga
     4021 ttacgattat accgatgtgc cggacggaac acgcatcatc tccgccggaa aattgacggg
     4081 caaagttggc gataactgga atgtggggct attgaacgcc ctgacagcgc gtgagttcgc
     4141 ccgcgttagt cccaacggtc tcgactcgaa aatcgaagct gagccgctga ctaattatgc
     4201 gattctgcgg acgcagaaag aatttcaaga gggtcgccac gggctgggaa taattaccac
     4261 ttctaccaaa cgcttttttc gggatgaaag tctcgccgac tatctcaatt cggatgccct
     4321 ggtagtgggg ctggacggct ggacttttct ggatactaat aaaacctggg taattgccgg
     4381 atggggcgcg atgtccaata ttcagggtaa tgagaattgt attaccgacg ttcagacgaa
     4441 cgcgcgtcat tattttcaac gaccggatgc tgagcattta ggagtagata gtaatgccac
     4501 cgcgctgact ggatttgcga ctcggattaa tctcaataaa cagcgtggca atgtcatttt
     4561 taatgccgca tttggtttaa ttgacccacg gttcgaggtt agtgacctgg gatatttatc
     4621 gagaggcgat gttatcaatt atcacgtcgg tgccggttat cagtggacgg agccgaatca
     4681 cttctatcgc aaatttaatt taattctggc tcacacactc ggtatggact gcggaggtaa
     4741 cctgactaat aatggtatta tgagtttcag ttatgtgcag ttctccaatt attattggat
     4801 tgattatgga ggcggctatt tttgggaaac ttatgatacc agacttaccc gcggcggtcc
     4861 aatagccata tctccatccg gaaggtgggg atatattcaa atatcaacag atgaacgcaa
     4921 accgttggta ggcaggctct actacgaaac cagcggcaat gacactgcct cggtcagtca
     4981 ttatttacaa attccgctca attggaaagt tgctgataat ttagcttttg agttaactcc
     5041 cagcatttct gttaatcata attatgccca atacgtggat gtcttcgatg acccctatgc
     5101 ggacgcaact tatggtaaac gctacgtctt tgctaagatg gatcaaaaga ctatatctgc
     5161 cagcctgcgc ttggactgga cttttacccc gcgtttgaca cttcaattat atgcccaacc
     5221 gctttatggt gcggcggaat attacgattt taaagagttt gcccgaccga aaacctatca
     5281 gttttttact tatgaagaag gtacggaaat taatagaaat agcgatggga attacaccgt
     5341 tgatccggac ggtaacggtc cggccggctc cttttcattt gacaatcccg actttaatgt
     5401 atatagtttc cgctccagcg cggtattgcg ctgggaattt gcgctcggtt cggcgattta
     5461 cttagtctgg acgcatggtc gctttgaaaa tagtagtgag gcagattttg acctaagccg
     5521 ttcggtcaat cgcatcgtag aaattaagcc ggataacatt tttatgatta aagcttctta
     5581 ctggttcaat gtctaatgct ggaaagattc tccagatagt tcgcccttat gggattaagt
     5641 aatgaaaagg ctaaggctga cgctgagaaa ttgaaatagg gagatggaaa tgtcatcctt
     5701 cgacaagctc aggatgacaa gaagcgataa gctcatcctt cgacgagctc aggatgacag
     5761 atggagtgtc actctgagcc atactgtcac tctgagcctg tcgaagagtg acaagatcgc
     5821 tgacctaaag tctcttgagg gactcatcct tcgacaagct caggatgaca agaagcgacg
     5881 agctcatcct tcgacaagct caggatgaca gatggagtgt cactctgagc catactgtca
     5941 ctctgagcct gtcgaagagt gaatagaagg tattaatttt attgggcaat tgttcgatta
     6001 attttgtatt acttctttta gctacaaaaa aatggcggca ttttagttgt ggatttttaa
     6061 attattactc ggaattggca aatagttaaa attccttatg taattacaga atggtaatta
     6121 tttacggtta aatattttgg agaattaatt tagtgacgac cgaatctgaa aatggcaatc
     6181 ccaaagaacg agaagccgaa cttattcaaa aaatccgcaa tcgtgtggcg gaagtgcaag
     6241 aattagttga tgaaatgaaa tcccgattga ctgacaaaaa atagtatttg ttgccttcta
     6301 aggtttgggt gcaaaagggc tcctttaata acaaaagctc taaggcacac agatatttta
     6361 gtaaaataga ccaagattga acgaggctaa ggttaggaat gaaaagccca atacccttaa
     6421 cacaagttgc atcagactat ctgcgttagg gtggggctgg tcattgatca ttagtcaaaa
     6481 cggctatatt ccaaacttaa cctaaaacaa gccaagatta tgactaaaac caggatgtaa
     6541 tatggtagca aagagattaa ataatctcta taaattagcg cataagtacg actttgatat
     6601 gggacatata gccgagcgcc gtcaagcggc agatataaac accattagca accagcgtac
     6661 cgttctggtc agtaccattc caataaacac tgtaactgcc ggccgcatga ttgccggtag
     6721 ctagcgtagt gaccttcatc ccgattaaat cgtaaattga tacatcaaca ggtccataaa
     6781 aaggaacatc atatctaatc aaagtctcta tattaaaagg attgggataa ttctgatgta
     6841 agcggaaatt cttaaatata ctggcgatcg agcgttgtgt accaacggtt gtgaaaacga
     6901 acgggcgttt attagtctga gaagcccagc attgattgcc gcggttgtct gtagccacaa
     6961 ctttccagaa atagcgagtg gcatagctaa gacgttcggg tggattataa accataattg
     7021 tatcttcttc cggttgctca aacataattt cacggaagtt cgtcaaaaaa aggctatcgg
     7081 ttccgtagta taatgtataa ttgatctgcg agcctggatc tttgtcgtgg gcgatatgcc
     7141 agacgaatcc ggcatccagc gcaaccgaat cctgcccgtt aaatggtgcg agtaaggcaa
     7201 atgggccagg tggtagatca acagcattta ctccaaaaca taccagctga ctccattcac
     7261 tgcgattgcc gtctggatca accgctgata tttggtaaaa ataatattta tcctctttta
     7321 attctgggag gtggagactg gttatgccgg ttttgctggc aacctgacta acctttttgg
     7381 gattatcagc ctcgtagaaa cgaacaaggt aatataaatc gcgttcatct tgatcggggt
     7441 cgggatcatc ggacggcaac cagctaatta gcggctgagc agtttccacc accgctgaat
     7501 ctttcggtaa aaaaccatcc gtaacaggat agggcgcctc acttatggca ttagtaatga
     7561 aggagttggt aggcgaccag agcgagtaac tgccgtgttg atcacaagct cgaacacgat
     7621 aaaaccagcg ttcgttttct attaaatttt caggaagagt aaaaacagtc tgccccggtt
     7681 cagttgtata gaccgaacat gcattttcgg ggaactgggg gtctctgctg atttctaact
     7741 ggtaagttag gctggcttga aaatcactaa aatcaggatc actactattt gcccagcgga
     7801 tttccgggcg cagggttttg attatagcgt catcagcagg atagaaacct ttaacaaccg
     7861 gcagaggtgg atcattttga tgattgaatg ccactctgac tggacgactg gctgatgttc
     7921 cactaatttc ggattgatca aaagcccgaa tgcgccagta atacagcgta ttatctttca
     7981 aaaggctgat cgccgaaatt tcatcaagac agactccaat agccctttgt ggcagttcaa
     8041 gtttagtttc tacctccttc gcagtaatag ttctgggaat ccggtaggaa aatatgggtt
     8101 cagtgaagtt aagagattca ctacattcaa tttgataatt taactgatcg ccgggatcag
     8161 gatcatcgga gggtaaccac cagaaatacg aatttggtgt gatttcctcg agattatgcg
     8221 ggtggataat tcgtactggt cgctgaggag cttcattaat ctgattgacc caaaaggtat
     8281 tggatttgga ccagacggag cgtgcctcag tcagatcgat ggaacgcaca cgccagaaat
     8341 attgacaatt ctccagcaga ggagtttgaa ccaatatttg gttctggtca taagtgcttt
     8401 ttagtaaaat ataggttctg ccgccgaacg agcgatcgcg acttaataca atttcatagc
     8461 gtaaacgatc ggcgacatca ggatcaatag ctggagacca tttcaattcc ggataatgac
     8521 tcgcgacgat gataccatcc ggattaaaac cctcaaccgg tggtagaggg ggttgattaa
     8581 tcccgtcgtc gaaacggaaa gtagcctcgg tccagttgga ttccgtcgta ccgccccatt
     8641 tatcactaac ccggacactc cagctgtaaa cccagccgtc tcgaaaaaaa tcttcatctt
     8701 gaattcgctt taagccaaaa cgcacttggc cattatattc gtaagacaag ccctcaacgc
     8761 cgccttcacg cgctttttct aatatagaat cgtttaaatg tactgtaatc atctgatccg
     8821 gatccagttc gttacgaata attatttcac aataaaagat ttcatcgcca tcgggatctt
     8881 cgcccatcat ccattccaga tagccatctg gctgtcggat ctgttgatgc gtcggagaag
     8941 gatcataggg cggataaggg ggtagattgc ctgtattatt tacaatactg cccaactgat
     9001 cggattttgt cgccataccc actgaatcat tgatcgtctc ggtggtgact aatttcaaat
     9061 aatcttcctc cggaggagaa agttgtaagt cggtgtaaag tgtgccgtct gtgagtaatc
     9121 tcggattggt gtcgctgaga attgttaaat ttttaacatc agaatcggta tcaggcatag
     9181 ccattgcccg gcaaattatc gcgattaata gacctaccga gaagattaat aatctgtgat
     9241 aactaacctt aaaatacaac acagcgactg gaatttaata atatagcgga gttggtgata
     9301 aagattttat gaaagaattt accatgattt ttaaaaaaaa tctcggacga atatttacgt
     9361 ttgacaatat gaattagggg tgatataata tcgaatagtt aacttctgcc ctacctacct
     9421 caccaatgcc caacaactaa taaccgttga ccaatgtcca cttctcaact gatcgcttca
     9481 ataaatttat actttattaa ggcaaactgt gcctatcggg cgaatattca attgcgaaac
     9541 actgccttcg gtaaagattt tggtgattaa tcttagataa tcactcagaa tttccttcgg
     9601 aataaaagcc agaatagttc cggcaaaacc gccaccctga atccgacaag cgcctttttt
     9661 ccccgccgtt tttaggaaat tttgagtcag cgctagcgct aaaggaacgc cttgttcggc
     9721 aggattcgag gtaggataac aattttgaag ccactgacag gatgaagcgc cggattcatt
     9781 gactagctcc agaaaagtgg cgaaatcacc tctctctaaa gcagatactt gttgttgaac
     9841 ccgctcgttt tcagtcagaa aatgaaaagc ccgcagaatt gcccggtcgc cgaccttggg
     9901 acgcagatcc ggaatttcct caatcaaatt ttgcattgtt atatcacggc aaacctgccc
     9961 acctaacgct ttggcaaccg cccgcatttc gcgagggatt gcggcatatt cttcggtcag
    10021 gttgacgtgg ctggttcctg tattaacgat tagccagctg taattctgct gttcgggatc
    10081 ataattaact ttctgaatga taggcttgga cggattttta aaatcaattg ccaccacgct
    10141 gccgacggca attgccaatt gatccatcaa gccacagggt ttgccaaagt attgattttc
    10201 ggcaaactga ccgatcaaag cgatatcctc agccgcaact tgtccatcgt tgaaaaggta
    10261 attcattatt gttccgagca gaacttcgat agatgctgat gaactcaagc cagatccgac
    10321 cagcacattg ctggttactg tggcgttgaa gcccccaacc cgcagaccaa gctctttaaa
    10381 gcgggccact atcccgcgta ccagagcggt ggtcgtgcct cgctcggctt cgaccggtga
    10441 gcaatgattg atatcaacga tgaatggttg cggataaccc tctgaaaaaa tggtaatctg
    10501 attcccatta atagcgttca cggccgcaat ggaatccagg tcaacgctag ccgccagtac
    10561 tttaccatga ttgtgatcgg tatgattgcc gctaatttcg gtacgaccag gcgcgctgaa
    10621 aaatagggca ggagaagtgg caaaaatttc attatgccga tcggcaagat acgcataacg
    10681 tttgatttgc tccgtaatta cgcttggatt atggccgtag actttttgaa atacggtggt
    10741 atctatttgg ttatgcggta agttagttat ctgatttttg ggcattattt aaccttacac
    10801 gtccttattt agagatttgt agtgaatttc acttgtttcc cggagtgtca gggcggctac
    10861 ttctggagtc agatcgcgct gagcttccgc cagcatttca tagccaacca gaaatttttt
    10921 caccgatgat gaacgtaaaa gtggcgggta aaagtgagca tgcaattgcc agtagtcgaa
    10981 gttaccgggc attgtcgggg cgccgtgcca gcccatcgta tagggaaaag atgattgaaa
    11041 aagattatca tatttagtca gaagagattt gagaattaag gcaagactat ttctctcggc
    11101 tggagtgagg tctggcaaac gcaaaatagg ccgccgcggc aggaggagaa tttcaaacgg
    11161 ccagatagcc caaaatggta ctactgccaa ccagttttca ttctcgagaa ccacccgctc
    11221 ccgagattct aattcatagg ccaaataatc agttaatagc actgatttat tctgcttaaa
    11281 gtaggctgat tgtcggcgat tttctttata aggttcgttg ggcaggctat tgaccgccca
    11341 aatttggcca tgcggatggg gattggaaca acccatcata gcacctttgt tttcaaatat
    11401 ttggacccag gaaaaatttt cacccagttc ctttgtttgt tcagcccaaa cggtaatgac
    11461 attccggatt tcctcaactg ccatttctgc cagggtaaga tcatgccgcg gagaaaagca
    11521 gatcactcgg caagtgccgg atacactctc atccctgata tacttcctaa catttcggcg
    11581 acgatgaggt gtatcgggaa acaaagccgg aaagtcgtta gtgaagacat aggtcgaggt
    11641 gtaaagtgga tttacggttc cgctagctcg gcgattgccg ggacataaat aacattccgg
    11701 atcgtattcc ggcatttctt cggaaaccac cggttcttga ctgccgtgcc acgggcgttc
    11761 cgcacgatgc ggcgaaacta aaatccattc gccggtgaga ggattgaaac gcctatgcgg
    11821 tatttgggaa aagtcaaatg acatttaaca attaattctc aagttcttaa gataatttac
    11881 cagaaaataa tgtaaagcac tatgaccagt ccgataacca tcgcgccgag ccattttaca
    11941 atcggcgaag attgcatatt gaagtctttg cgttccggca tcgtcaccgg ctgatccaac
    12001 ggttttaacc aggtaataat ccccattatc actacgatga tgataaaagt gatcgccatg
    12061 cggtttagaa aggcgattgg gacaatttga acactggcaa agaccggtag agaaccgcaa
    12121 aagatcagaa gaagaccata gattatggga ttaagaatca gcgcggccaa gccggcggct
    12181 ttcggggctc gcttgaatat cagcccaaaa gcaaacgcag ctagaattcc tggcgaaatg
    12241 aacccctgaa attcctggag gtaggtgaaa attcctttca gactcggatt atcaagaatg
    12301 ggtgcaatca agcaaccgat aattaaaaaa acgaaagtgg aaatccgtcc cacccgaacg
    12361 atcttttgct gagggagttc ctttttaacg gcatgctggt aaatatccat cgtgaaaata
    12421 gttgatgccg aattcagcat tgacgccagc gagctgatta cagcccctcc gatagcggca
    12481 aaaataaagc ccagaaatcc gttgggaatc aaattcctga caattgtcgg aaaagccgca
    12541 tcggtgccgc ccgggccggt catttgatct ttaaataact gccaagccat aattccggga
    12601 aaaacgataa taaacggcgt aattaacttt aaaaaggaag cgaatatcac ccctaactgt
    12661 ccttccctta aatccttagc ggcgagagta cgttgggtaa taaattgatt gacgctccag
    12721 tagtagaaat ttggcaacca cagaccgata accagagccg tccaggggag cacgggattg
    12781 tcgcgcggca aaatcatatg caatttatct tgattagcct ctaagaaacc actaagccct
    12841 ccgacatgtc tcagtccgat taccaaggtc aggaatccgc cgagaattag gcttgaaccg
    12901 aagaagaggt cggcccaggc gattcctttc aatccacccc aaatagtata aatggcggta
    12961 attatgccga ttaaccaaat tgctcgggtc agagtgacct cgatcccaaa aaatttcatt
    13021 ccggaaaata aggtggcaat agtcaatccg ccggtataaa taaccgacgc caccagcaca
    13081 aaaacgtaga ttaaaatagt gtaaacggcc attaaatggc gcgccagcgg ggagtagcga
    13141 tattcgagaa attccggaaa agtgtaaatg ccggtgcgca aaaaactagg caaaagaaac
    13201 agcgccacaa ttaccatcgt gatagccgcc atccattcat aggaggcaat cgccagtccg
    13261 gccgagccgg ccgcctgccc agccatcccg acaaattgtt cggtggagat attagccgca
    13321 atcattgaaa ggccaatcaa tggccaagtc aattgtcggc cagccagaaa gtaatcctcg
    13381 cctgtttttc ccttacggct tttgtacata ctgaatatca ccacgaacaa gaaaaacagc
    13441 atgaatatta agccgtcata ccaactaaaa actacctgtc tcataattac ttatccccct
    13501 ttttatcaaa ttttaattgt gctttttcga agccgtcaca aaatcccctt tgtccggctg
    13561 ccatcggctt tgcctattat ttttgtgata attcaataat cttttttcct gtcccacttt
    13621 gccggttcgg gcaaattaac ttcattggtt accgttctgt agttctgatt gagttgaaaa
    13681 ttacctctat ttaatttaat gttcattttc tcgtttattt caattaaagc aatttagcta
    13741 aaacgatcgc tatcccaaaa aggttttttc actaaatttc aacattgaaa aaattaaaaa
    13801 tagcaattag aaataaaccg taaaggatat tgcttaccac attggttcag gtagttagtt
    13861 tatcgtagag ttccccaatg gcgcttttta gttgttcttc ggtgccgttg ttttcgatga
    13921 cgaattcggc gagttcgcgt ttgtcttctt ccggcatctg cagttcaatc cgccgcagga
    13981 tatcttcgcg ggtaaggtta ccgcgacgta aagcccgttc caaacgatac ttgtatttgg
    14041 tgtagattag caaagtgtga tcgagccgac tgtcgaaacc ggactcgaaa ataaggggtg
    14101 catctaatac gattaagtcg tacttatcgc gcatcttatc cacttcttgc tgaaaataat
    14161 ctattacacg cgggtgtaca atgtcattta gaatgcgttg atcggcttcg ctggcaaagg
    14221 cgcgatgagc taatttttgg gtgtctaact tgccgtcttt ataaacatcg ttgccaaaag
    14281 ctgcgatgat ttcgtcacgg acttggggat cgttttccat taattgtttg gcaattaaat
    14341 cggcgtccag aacataagcg cctttctctg ccaggaattt acaggcggtt gattttccgg
    14401 agccgagtcc accggtaacc cctaaggtaa ccacgttttt ttatcctcct attacacgat
    14461 ttattctttg gaatacttca tcgggcgtac cgtcagccgc gattgtttta agttttccac
    14521 gctggcggta gtattctgcc agcggttcgg tttgtttagt atagactttt agacgtcgct
    14581 ggatggtttc aattcggtca tcatctcgtt gatataattc accaccgcag atatcgcatc
    14641 gcaggggatt tttaggcgga ttggtaatga gattatagac cgcgttgcat tggcggcagg
    14701 tacgtcgagc tgaaagccgt ttgataatag cttcgggctc ggcttccaac aaaattacag
    14761 cgtcgagatc aacgttatgt tgcgcgaaaa gccggtctaa accttccgcc tggggaatag
    14821 tacggggaaa tccgtccaag atatagtcct gcggagcatc atccccgaaa agcgtttcgg
    14881 caattaagcc gagcatcaaa tcgtcgggaa ccagttcgcc gcgctttata taatcttcgg
    14941 cttgtttgcc cagctccgtt tgatttttaa tggcttggcg caaaatatcg ccagttgaaa
    15001 tctggcgcag accacgttcc tgctgaagtt tctgagcctg agttccctta ccgactccgg
    15061 gaggccctag taagattact ttcatttgat ttcctctatc caattttgat ttattttaaa
    15121 atatttttcc taatgtgtca actataataa caatccagca attgtagccg tcatccagga
    15181 tgccaaagcc ccgccgaaca tggctttcag agcaactttg gataaatccg aacgtcggcc
    15241 aggtgctaaa gagctaatgc caccgatttg aatgccaatg gaagcgaagt tggcgaaacc
    15301 acacaaggca taagtcgcaa tcacgatgga tttctgatcg agagcacccg tgtgaattaa
    15361 ttcggaaaga gcagaatagg ctacgaattc gttcagtgaa atttttatgc ccatcaagtt
    15421 accaacctcg ccggcgtatt gccaggaaac gcccatcaac caggctagcg ggcgcagaat
    15481 aaatgaaaat aatgacttca gactgcctgg gaaaatgccg ctgaactcac ctttgaccgg
    15541 ggagaatccg gaagagttgg tataaatcgt ataagcgcct ttcagtaaat taccgtcaat
    15601 cagacggtca ataaatccta aaatggtatc aactagacca attaaggcaa taaaggcaat
    15661 caacatcgcg ccaacggtag ctgctaattt tagtccgtca gtcaccccgc gcgccgccgc
    15721 atccagaaga ttagccgatt tgacggtatc ttccggtaaa tctacgtcgc cggcggtttg
    15781 gggatggtca gtctcagggt agatgatttt cgcaatgacc aaagcggcgg gagccgacat
    15841 aacgctggcc gcaattaaat gaccagccgg tacccccatt ttgatatatc ctgccaatac
    15901 gcctccagca atcgtggcaa atccgccaac cataatagtc agtaattccg aaagggttaa
    15961 ctgatcaaca taggggcgga ttaataacgg ggcttcggtc tgaccgacga agatattggc
    16021 agtgcaggac agtgtttcgg cgccgctggt gcccatcgtc cattgcataa aacgcgccat
    16081 cgccttgata atggcttgca taacgcccaa atgatagaga attgccataa acgaagcgaa
    16141 gaaaattatg accggtagca ccgtaaaggc aaattgcatt ccgaatcccg gccaggtggt
    16201 ttcattcggg aaaaaatatt tatcgagcgc caggttgccg aataaaaact ctgcgccgag
    16261 agtcgccaat cccagaaatt tttcgacttt ttgtgaaagg aaagcaaaaa tatcctgccc
    16321 agtatagccg tgtaaaatca gccaacttaa tccagccgtc aaccaagcga cagttaattc
    16381 cttttgccag ggacgaactt taaaaatagc cttgataacg atcgctacgg taattaagcc
    16441 gattaactct gccaaccagc cggttttccc aaaccagtaa aaagcagctg tagctaaaac
    16501 ggtcgctata cttatcaaac cgatataaag ccattgtttg gaccggtgac cgaccgtatt
    16561 aatttgatct ttataaatga atattattat taagaggaga aagataaaca gggtaaagta
    16621 ggaaatcagc atttccccca gaattattcc ggcaaaaagc agttggagta agagccccca
    16681 gataacgact cgcaaggaaa ttagccgtcg gttattcgac atcagaaatg caattcccag
    16741 aatgacaagt agtccaatta tgcctatata tttcatattt acacctaatt agtcaatata
    16801 gttgttaaac cgagagaact cattttttct cttctataat aggtggttca aagcatgctc
    16861 gacaccgggc ttcgtatata tcggttgcgc caagcaaaac ccgctcgctg gacttgatga
    16921 tgcgctgggt gaaattggct gggttgccgc atttcataca gattgccagc gtcttggtaa
    16981 tatactcggc ttcacagagc agttgcggaa tcggctcgaa cggttcgcca cggtaatctt
    17041 tatcgagacc agcaacgatc acgcgtcgac cggagttggc gagtgttcgg cagaccgcga
    17101 ctaattcatt atctaaaaat tgggcttcgt caattccaag cacttgggca ttgccggctt
    17161 tttggagaat ttcctgagca gtatggatcg ggatggaggg aattttctgc tggttatgag
    17221 agacaatata atcttcgcta tagcgcacat caattaccgg cttgaagatt gctacgcgct
    17281 ggcgcgcgat ttgcgcccga cgcaaacggc gaattagttc ttcggttttg cccgagaaca
    17341 tactcccgca gatgacttcg atccaaccag ccgatttttc ggtaacggta actacttctt
    17401 cgaagtcggt gggaacacgg tgtaaatttg gttggctcat cctgctcctt attatcgtgg
    17461 ttcgttcaag tgccttaaaa ggcttcttac tttagtttta aaaagatcaa tggctaccga
    17521 gttctggcca ccttccggaa tgatgatatc ggcgaaatgt ttagtcggct cgacaaattg
    17581 ttcgtgcatc gggcgcacgg tttcaagata ttgggcgatt actgaatcaa ccgttcgtcc
    17641 gcgttgatga atgtcacgcc tcagccgtct aatgaaacga atatcaggcg cggtttcgat
    17701 atagactttg atgtccatca tatcgcgcaa ttccggatca tacaaaacca taattccttc
    17761 cagaacgata attttctgac catttacaac ctgagattca gttttactgc gagtatgagt
    17821 tttatagtca taaatcggga ttttgacctg ttgaccgttt aatatcgcac tcatatcagc
    17881 ccgcaactgc gggaaatcgt aggcgtccgg gtggtcaaaa ttgtgatgac cacgcgattc
    17941 caacggaata tcactgagat cataatagta ggaatcctgc tcgataatga cgacatcgcg
    18001 aggatcgaaa tcatgtacta tatttcgcgc catcgaagtc ttgcctgagc cggtacctcc
    18061 cgccactccg atcaatattg cttttttcat ttatttatca attcccttca tctggttgtt
    18121 atcgaatggt atcgggaaca gctcggaact gcggaaatgc tgatagtcct ccgattcatc
    18181 aaaaagaaaa atatcaatgt cgccgcaaat atcccaaatt acttgccgac aggcaccgca
    18241 gggtgatacc cgggttagcg aaaatattcc catagcgcga aaatatcttt caccagccac
    18301 aatcgctgcc gcaatcgcat tgcgctcggc gcatactgtc aagccgtacg agctggcttc
    18361 tacattacaa ccggtgtaaa tcttgccgtc attagttaaa agtgccgcgc cgaccgggaa
    18421 gtgcgagtag ggagcataag cgttattggc ggcttgccta gctgcgactt ttagtaattc
    18481 tggtttaatt ttttccacgt tcttttcctt tcatagggtt ccagaattag caataaaatt
    18541 attccggcta aaaagccacc aatatgggcg aaccacgcca ctccaccctg cgtttgaaat
    18601 cctaatgagc ctaaaccatt gctgagctgg acaataaacc agaatcccag caaataacca
    18661 gcaggaatcc agatctgacg aatgaatata aaaaaccaga agagaacctt tacccgagcg
    18721 tgcggataac gcaggagata agctcccaaa actccggaaa tagcaccact ggcacctatc
    18781 atcggtatcg tagatctagg agtaattaac acctgagtta aaccggcaat tactccgcag
    18841 agtatataaa ataacaaaaa cctaccgtgt cccagggttg actctatatt gtcaccaaaa
    18901 atccataagt agagcatatt accaaaaaga tggagaaagc cagcatgtaa gaaaagcgtt
    18961 gtgaaaaatg ataaaaaatc tctcaggctg gggtgactaa atagattggc agataccaat
    19021 ccatacgtat tgaggaatgc aactaaaatc tccggtggca aagtaagctg gtaaatatat
    19081 acgaggcaat tcaaagtaat aagtccgaca gtaacgtaag gaggagtcag gcgcatagta
    19141 gaatcgcgaa gtgggatgaa cattatttct cttaacctgg tattatcgta aaagtactca
    19201 ctgcagtttt tacattgcca gcccggtcgg taacggtaat ggataaggcg tgtttgccgg
    19261 ccgtaagttg tcggctcagg cgatatctaa tctgattctt tggggcatta tactcggcga
    19321 ttattggttg cccatcaagc gtaacaacga tggattgatc atcagtaatg cctgagagtt
    19381 gatcgtgaac agtagccttg agataatgca catcattggc gtgaaaccgg ccaccattgc
    19441 ctggaaaaat gttgtgaata accggcggcg tatagtcttt tataagagca aattcaccgg
    19501 tctgtcggct ataagcccag ataaaatcct ggttgacatc gagttggttt ccccgaaact
    19561 gccagttttt atcaacgtac tgataaattc caacttgact gatgtcctga gcacatacag
    19621 gatttttaaa gcatacctga attgaatcgt gcaaaggctg gttagtggga tgaagtccat
    19681 agcgcgccga tatcattttg ccgcctttga tctcggtagc ctctttagtt tccagccaga
    19741 caagcagtga atcgaagacc gcatctctgg gaaaaactgc tttgaagacc gagtcttggc
    19801 taaggcagaa attttcattg ctgggtacta tgcatttaag atttaatcgc ccacgtaaga
    19861 tattagttaa ttgattgtca aagcggattt cataggcaga ggcgtcacgc caataggcaa
    19921 aaggcactgg cacagtcata tattcggtcg gggaaatccg gattagctcg actttcccca
    19981 gcccgttaat ggtctgaaga tcgaaagtcg gattgctatc gggaacggcg ctgaaagtca
    20041 ggagcatcaa gaaatttttg ggattgtgga tgaatgtata attcaatgaa atcaccgagg
    20101 atttttcggc ttgattcaag ttaataaata atggttgaaa ttgattgccg tcaacgccaa
    20161 ccgcctcgat tttaagatat tcattatcca ctggttgatc aatcaatgat aatttataaa
    20221 attccgcagt cgtatcagtt aatgcaagat tagcctggcg aagataatgg ccgtttttat
    20281 taacccagcg agctttaaaa ttttctacaa tcggctgggc gggacgcgat agaaacagtt
    20341 gataaccttc ttcgccggtt tgaatttcta ccgattcaaa ggaagtaaca ggagcggaaa
    20401 ggattttgaa gtccaccata gcctgattct gatttttatc ataagcaatt atgcgcactg
    20461 aatgtaatcc ggtctccaga tcaataatac cggtagcctc cggcagggta ttcattggca
    20521 tatttttatg tggtgcaatt gtccataaac gattgtacac ttcacccagg tcatgatcca
    20581 gctggtaatt gcggtctatg atggcaaagt gagtttgctc gtagctgaat cggtcatatt
    20641 gaacctggaa atagaggctg tcgtcaataa atagtttaat accataaggc gggtaaacat
    20701 ttgagacgcc cttgatcgta tcgtgaactt ttatttctat tccgaattgt ccttcaactt
    20761 gaatcggctt tttaagaatg tggtgattaa ttttcttttg aatagtttta aaggtttgag
    20821 tcatcggcaa tccgttaata cgagcatcga gacttatggg cgtaacggca atgccgctga
    20881 tggttggggc agttttatcg ttcaccgtaa ggttagtgtt cagaggatta aaaggattat
    20941 ccaggctatc gcggagttca aaatgcaggt gcggatgaag agtcccagtt tcgccggtat
    21001 atccgatgat ttgtcccttg gtaacgcgca aatcatcttg cttaagatta acttcaatgc
    21061 ggtaacgctt ctgacgctcc tgttcagcct ttacaatagt tgagagtggc tctgcgaaat
    21121 cgtccagatg agcgtaaacc gcgatgttgc cgtcatccag ttggtggtaa attactttgc
    21181 catagcccgt aaacgaggtg cgaatagttt tgatataacc gttctcgatg gcaaaaaccg
    21241 gatagccggt gcgttggttg gttttaatat cgaggccggc gtgtagatga ttgacacgat
    21301 attcgccgaa agtcgaacta ataagccggc tggagttagt tggccagagg taattctgag
    21361 aaagtgtggt ttgaaaaagg agaacaataa ccgaggcgag tgggaaatat ctcatgccgt
    21421 aaaactaatt ttcatttcgg ggtggaatat aagaaaaatt agctccaatt ttcaaatttt
    21481 atttagtaag taatcggtag catttcaatt aggctaaacc ggaataatca aagattaccg
    21541 atttattacg cccactccgt ttggcttgat aaagggcttt atcggcggca tcaaccaatt
    21601 gggtttcatc aatcatccca gcgggattaa actgagcgat accgatggaa attgtaatac
    21661 tgacaacagc ctgttcaaaa taaattttcc ttttggcgac gatcgtacgg attttttcag
    21721 ctactaagaa agcttccttt tctcccgtct ccggcaatag gacggcaaat tcctctccgc
    21781 cataacgggc acaaaagtca tttttgcgta aacaggtcaa caaaatctga cccaatgttt
    21841 ttaaaacctc atcaccgaag agatgtccgt gagtgtcatt gacaattttg aagagatcaa
    21901 tatcaagcat cagtaggctc aacggtgaat tgtagcgtgt actgcgagca gtttctcgtt
    21961 ttaattcctg gtcaaaatag cggcggttat aaataccggt tagagcgtcg gtagtcgaga
    22021 gcgcttctag ccgccgattg gcttcttcta aggcttgagt gcgttcccga acgcggctat
    22081 ccagtagagt atttaaatcc tgcagagccg agcgggcaga tttaagctct tcgatagcct
    22141 cttttaggtg taactcgcga gtctcaaacc tccgaagtag attgtcgagt atcgttgcca
    22201 attggcgcaa ggaattcggt acgccgattc gcataaattt cttaaaggat tcatcgttat
    22261 attcgccacg tgagactgcc tcaatctggc tgacgaattc ttcgaacaaa ttctggtccc
    22321 aatctttcat tttcgacagt tattttatga atggtttgta ttgctggaat cccatatcaa
    22381 tggaacgaat ctaaccggca gaatcttctg gctgtttaat cccagcggtg tttttgcgat
    22441 taaagtcaat tcctgaccac cgcaggaact tccgagagga attaccagcc ttccgcccaa
    22501 tgccagctgg tcaaccaatg ccggtggtat ttcgggactg gcggctgtga cgattatatg
    22561 actaaatgga gctttctcgg gccagccaag acagccattt ccgattcgga attctacgtt
    22621 atggatgccg atctgggaca gtactttttg agcgcgctga gaaagttcac cgattcgctc
    22681 aatggtgaat acattgccgg ctaatcgggc tagaattgct gtttcgtagc cactgccggt
    22741 gccgatttcc agtacccagg ctgatttatc aatttgcaag gcggcaatca taaaggctac
    22801 cacataaggc tgagaaatag tctgaccgta tccaattggc aggggttggt cactataggc
    22861 cagcgttcgt tgagtgtcat caacaaagag gtgacgcggt acctgtcgaa aagcctccaa
    22921 taattccggg tctttaatat tgcgggctaa aatttgttcg gcgaccattt tgtcggctgt
    22981 ctggcaatta tctggtgaaa aattgttcat tggtccgatt gacgcgaaat agattccagg
    23041 agatgtttta cgtcgcgtag agtctcgatt ttgacgtctt tcagataatt tagatgaatt
    23101 gtcttaaaag tcgagttgat ggcaactcct ttgataaatt gaacgagcgt cagcggaata
    23161 tgcaaagttt tcaaggtgat gcaatttaga aggtattcag gatcttcagg ggccaactgc
    23221 gctaaagtag ttctggtgct ctgtaatgat ttacgccgtc ggctgatctc ctgtaggtct
    23281 gtgaattgga cctggtaagg atgaatcgga cgcagttgag cattcaacca ctccagccca
    23341 gatttgaagc aggtttcaaa aaaattgcgg taacggcgcg gcccaatatc attctgaagg
    23401 taattcacta tcttttgata aaattgacct cgataatttt cccactgttg aaaatgactg
    23461 cgaagtaaaa agtagccatt gggcgtcttg atactgtttt gattattgaa aaaggcttta
    23521 atttcgtagc gaaagcgctg aagccgacga tgatgttcgg gagggaaaag gttaaagatt
    23581 ggcgattctg gacgagcatc atcgcgaatc atttggggtg gaatctggaa gtcgctaagt
    23641 cggagcgaat atgatttatc caatccttga aaagtcaaat caatcaaagc gccaaaatcg
    23701 gctaatttaa tgaagttgtc aatttcctgg ttgaattcgg gcagggtggt aatcggcggc
    23761 agataaatgt cttcgaataa atcggggaaa agaggcatct gttggggatc gcaattctta
    23821 tacattacgg attcctgtta aattatcatt ccctccggga ttgtagtgtt tttaggaata
    23881 acgacaatcc catcccggat cgagtagagc tcactgtcat attcttttag ttgatctcga
    23941 tttacaatct ggacattgtt accgatgcgg gcattcttgt caattatagc atttttaatt
    24001 acacaatttt ggccaattcc taattgaggc gaaccttctg gctgaacctg ttcgaagtaa
    24061 tcagctccca taatgacgca tttctcaat
//
LOCUS       DAOSMP010000035        23425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_2193_length_23425_cov_21.235002, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000035 DAOSMP010000000
VERSION     DAOSMP010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 23425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23425
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2193_length_23425_cov_21.235002"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1754
                     /locus_tag="PKX09_06985"
     CDS             <1..1754
                     /locus_tag="PKX09_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOU17601.1"
                     /translation="ATQRHSVAQQFRLAGYEPHKWEPAECLAILQLFKWAYLSQWNEK
                     IVIYKTGEVFGDERTRDGFPILEQSSAIRESGYQNTFFASLNQLYWDGVNLRQAMNLF
                     PNKDKSLAWILAGKNVPADQTRLIFENNWFNSYDFIFDMAVLDRRMVGLFFPGVPICL
                     AGCNQQVAFGTNWLPENEVNLLVVRINPASEQYLAQDGWHNLRKRSTQIAVRSDHKQE
                     VTFYHTDQGVILDYPIESVDTVAYAIVPKWQAVTASEINQRVALMLIGNNDAARVMTE
                     VSSNLPGLVYLDQKGNYGSSALPKPLTPNLSINCLTPFTIDDWRFSSQFTIDSLKSHF
                     LPDWQDQPKALFSESTDEFFPILATDKYFTRLIKEVRAALPDTIFTRKTELQAYQNLI
                     KWDGRYAKTAVGPTIYQAFLKALMRNIYSDELNLVDSETFEQFANSGDFAVQNLLVLI
                     EKGESNWFDNLRTSDFVEWQGEIIRQSFKEAVQLIEEKYSPNLSEWQWGRVNQDVVLN
                     NNIKRNKLSNSKSVSLNSSGAIQTIILKPAGSETACCIAVNGTTNPVEPVNLLSDRAE
                     LLRNGAYIMTLKASPKK"
     gene            1765..2781
                     /locus_tag="PKX09_06990"
     CDS             1765..2781
                     /locus_tag="PKX09_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HOU17602.1"
                     /translation="MLEVIKSTIMITGFVFTMMLVIEYLNVQTRGKWHKLLNGRKWRQ
                     YVLGALLGIIPGCLGSFAMVTLYTHRLVSIGTVVTTMIATSGDEAYVMFALFPGKALL
                     LNVILAIISIPIGVLADLLFRKKYDVITAHDLPIHDEYCECFQRGKILEQLRSITLER
                     FLLILIMAAILLAAASGFIAGEEEGWVRITLIITSIVGLAIVATVPDHFLKEHLWEHV
                     TKRHVPRIFFWTLGALIVIQILTNYLNIEHFLQLQQARILVLLIACLVGIIPESGPHL
                     IFVTMFAEGLVPFSILLASSIVQDGHGMLPMLAHSRRDFVRIKAVNFGFGLLVGLIGL
                     WLGF"
     gene            2812..3459
                     /locus_tag="PKX09_06995"
     CDS             2812..3459
                     /locus_tag="PKX09_06995"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOU17603.1"
                     /translation="MNKAFTFLDLTHTLENGMPTFPGDRPKPRIITRSKTQFTISEIH
                     LGTHYGTHIDAPRHFLPDGKSIADFNVDRFTGAALGLHRDSAGTSALNLNDKEIELIQ
                     NLEPQWLILRTGFDRFWSQPNYFLEHPYLSVEFAQKIIDLKIKGIGGDFPSMDAANAA
                     RQNFPIHHLLMEHEVLIIENLTNLSALPTQELFHLIALPLKIATDGAPARVVAWF"
     gene            complement(3620..4174)
                     /locus_tag="PKX09_07000"
     CDS             complement(3620..4174)
                     /locus_tag="PKX09_07000"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOU17604.1"
                     /translation="MGQIKSAPQGHLFLAIMSASEHQRQVALEEVRTRFGEIMGAGPL
                     FSVSDFTNYYESEFGRDLKKQFFIFKQPTSLENAHQIKVWTNRIESQFVDNSGSQSRR
                     QVNLDPGYLEPSKLVLFSTKNFSHRIYCGDGVFAEVTLIYEHGQFKILPWTYPDYYWT
                     KNLEFLIQMRNEIVRLSRKDSKQA"
     gene            complement(4161..5549)
                     /locus_tag="PKX09_07005"
     CDS             complement(4161..5549)
                     /locus_tag="PKX09_07005"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU17605.1"
                     /translation="MSDRSLLLINPWIADFTAFDLWSKPLGLLYIAKFLQNYGYEIVL
                     LDLMDRQRWQDTSNDKFNDGRGKYRKTVIPKPPVLARYPRRFGLYGASREQFSDTLSN
                     MARPNAILVTSLIAYWYPGVAATVEILRRYFPETPLILGGIYASLYPEHARNSIQPDF
                     LITGYGEKKALRLIDHLFGIERDYGDIPDYNDNGILPWHLYPKLKSVAVLTSRGCPNR
                     CTYCATYLLNKKFVQRRPEDVISEIISTYNQFNVENFAFYDDALFAHRDRHIIPILKG
                     IISAGVKINLHSPNGLFAREINSELAELMFSAGFKTVRLSLESTSSQIQKSSSNKVNN
                     ANYTDAVNNLAKAGFARNSLETYLIMGLPGQTRQQIIDSINFVADQGVIVKLASFSPI
                     PGTIEWERARGLGYVSDDQDPLMTNATIFPFLSRNLSYEDYLALRQVANERNAENRGN
                     NQISNEASDGTD"
     gene            complement(5749..6654)
                     /locus_tag="PKX09_07010"
     CDS             complement(5749..6654)
                     /locus_tag="PKX09_07010"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOU17606.1"
                     /translation="MRQKLRTGFIIIITGMFLQAESGESGMAFLKIPVSAQTASTMGV
                     FAQVSGTPFTIFDNPIGIINDDIRIGFSHNFWFADVSGDALAATIPLKKGNLGFGLNF
                     VKIPGIEIRDIPSDESLGEVEAQYMSAAVGYAYPIFSKLSIGCTVKYLYEHLYAENAG
                     GVAFDIATHWQIPSGMDLALSLSNIGQMQTLKDKATTLPTLFKIGIVRPEIFADSPFN
                     VNLGINLIANLVTEKTYFQVGGEIAFTDLIKIRGGYERVGTINRTALGAGLNFGRFEI
                     DYAFIFMPDGLGYPNLLTLSYRPEL"
     gene            complement(6663..7589)
                     /locus_tag="PKX09_07015"
     CDS             complement(6663..7589)
                     /locus_tag="PKX09_07015"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOU17607.1"
                     /translation="MRKLPNLILSLIFSMALLQGETNDNAGYAGAFLRMGLGARSTAM
                     GNSGVAFPSNGFGIYYNPAGLAYMDERNLALSYSFLSLDRQFHFIGLSVPLPPNAGVG
                     VSWLHAGVKDIEGRNSIGQVDETYQTGENVVLISFANAFHPRISLGINFKILHNQMLD
                     VSATGLGFDFGVLYQPLEWLTAGLQFKDIGAGYNWNTQDLFGEEGSSYKENFPQILKF
                     GLAIKPIQELTFVGDVGVSDQNVWQTHCGAEYVYRNLAYLRIGMNQLSPTFGAGLAYG
                     FIANMTTQLDYSFIIGAVGEGTTHIFSWQFQF"
     gene            complement(7592..9163)
                     /locus_tag="PKX09_07020"
     CDS             complement(7592..9163)
                     /locus_tag="PKX09_07020"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU17608.1"
                     /translation="MKKYPAILFIIGFLFTSGFSQAFRLAKPTLDSDNTFLGLASNSV
                     TEIVSQGDSLMWFATGGGLSMSPDFGQTFVSFYPGLNNLPRGGISAIAVKGDTIWVAA
                     VFDSLIQSYGYQQTGAGLSYSTDKGQNWTYVPQPIDDPTDNSDEWNNQIVSFLPVTTP
                     INNTTWDIAITDDYVYIVSWAGGIRRSADMGQTWQRIPLPSDDLNNLDCDDLIDFEIN
                     PRDPPDGNHNHKGFSILAYGDTIWIGTADGINLGIVESPECISWRKFNAQNSAISGNF
                     VVAIDRQLWKGKETIWAATLTVGESGEYRAVSKTSDGGLTWTTTLVNERPYNFAFQDS
                     IVYVCTERGLFKSIDGENWAIYTPIADIASGEQILTQYFYAAEVDRREGPTYLWVGTG
                     DGVAKTASDGLTWTVYHSAVSTKSPGQPAIYAYPNPFAPRYQNVIGDDGHVRIQYNID
                     QPASVRLEIYNFAMEQVYRSDWKMVMDAGDHHLIWNGRATNGELVANGTYFCKLIQKS
                     NGNEVEHWTKLIVIN"
     gene            complement(9160..>9644)
                     /locus_tag="PKX09_07025"
     CDS             complement(9160..>9644)
                     /locus_tag="PKX09_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17609.1"
                     /translation="FFDPYFYLNDAGEYGDEKARDGIFTVFQPSDPESKYQGLYTFYF
                     FAEDYSGARSDTITLKITSPGIHITAPASALTLCFGQTLRIMWEYAYISQVKIEYTFN
                     ANETNPDWIVADTTAAALKYYDWTVPATQVTDYCKVRVSDLTKPARYDVNDDYLVITQ
                     "
     assembly_gap    9645..9744
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9745..10382)
                     /locus_tag="PKX09_07030"
     CDS             complement(<9745..10382)
                     /locus_tag="PKX09_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17610.1"
                     /translation="MKRIIIAALFITILTLWTCENKSIVETVEPTPIIENLTLHYSEL
                     NHSLFIAAKVEDDQGWENLDSVVFNLYRINTIDSMNGFLFKSGKLSDDGPPRDIITRD
                     GVFSYLIDSTELGSYEGYYRVFVWAYDADGNVSDSATATELVQANSPPEIFLLSAPTT
                     FEKWDTLKFYFRVTDLQGYDDIVAVYYSILRPDGKFFDPYFYLNDAGEYGDE"
     gene            complement(10383..11207)
                     /locus_tag="PKX09_07035"
     CDS             complement(10383..11207)
                     /locus_tag="PKX09_07035"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOU17611.1"
                     /translation="MIATYQRWLKVLIPKSAGNEELIIARLIQWGMLGLQETDTDYEI
                     YFPLNMKLSLPELIAAEWPQLNITITEIVDNGWSTRWQEYFKPQIITPRIAVRPYWEK
                     PLKDVPVEIVLKPGMAFGTGTHPTTHLALLMLEKYLTPGNSLLDAGCGSGILTIAALK
                     LGAAQVSAWDIDSLVIDNFNENLELNGLSRKVAIQIGDVTALPNYNYDLIVSNIERLS
                     NLRLLEALVHQRNRPLVIFTGLLKEENEIFTNKVLNYGREILDQDTEDEWLALVIK"
     gene            complement(11213..12127)
                     /locus_tag="PKX09_07040"
     CDS             complement(11213..12127)
                     /locus_tag="PKX09_07040"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOU17612.1"
                     /translation="MKKSFYLILILISACSSGGGKFDGGRAFQFLNQQCDLGPRNPGS
                     PGHSAQRTMLIEFLKPLADTLIIQDFEYLIPEENKILNLTNIIAGFNIRSTNGLLIGA
                     HWDTRPRAEYDPDPAKRETPIIGANDGASGVAVLMHLAEILSRQQPPRTIYLVFFDGE
                     DYGFPGTLDYYCLGSEYFAKNLPIPKPEEAIIIDMIGDADLRIPVERNSVKSHPQLIK
                     KLWAIADRHNFSAFVHQIDEEIYDDHLMLIQHAGIPSVDLIDFQYPNRFINYWHTTLD
                     TPDKCSAESLEIVGTVLYEYIFYGSEER"
     gene            complement(12130..>15170)
                     /gene="sprA"
                     /locus_tag="PKX09_07045"
     CDS             complement(12130..>15170)
                     /gene="sprA"
                     /locus_tag="PKX09_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOU17613.1"
                     /translation="DLGIAKDDTSIFVVNENSFSISVINTEDNPDIYEPPAGVQGEYD
                     EINQIRTKEQALVLNLPEEGSGLFPGEFCAAKKVLAEETSYITYKVMKLFVNGHELKA
                     DGYQFSEGEETPLQFFIRFGKIFENSPTTTNYYEYRQPIYPGWAEENEMELDLDFLTS
                     LKNYESADDYIGEGEYQIYTNTSGVTIRHYKEKLNDAYTGREIIIYGKPSLANVKHLN
                     IGLRNRADNSGYVNKFKTGTIKGQVWLDELRLSEVRKDKGIAYRAKASLRVADLASFD
                     VSVNYRDADFHTVEQRPSLQTENLKTTEALTATGRISLDKFTPASWGLRLPVSGSYTQ
                     TTGTRKYIYGSDILMKENAPDSLLDISHSYGVNTSIEKRASDFWLTKYTIDQIKISAN
                     ATWTDASSVTVRESQSESYKASISYNFPFPKDKYINIFKWTAPVPLIGNKLSEIRFYY
                     FPNALTVAADAGRSESYSTPRYGTTITSSNSFGLNRNLSLGYKIFDNLSANYTLNLEN
                     NLIELWQNQWEILKSANPGQITNMRESYSVNFNPKIAKWLNPTFNYTSNFNWNEPIVS
                     NSASVDAISNQNRLTSTFSLDPSAIFSSLFKPSGTAPAPSPTPTPPGQTTGRRRATQT
                     GAQNQPKTAEPAPITGDSTRTDQKSGEKRPRKPGSTIDKGEDLKPTSGQTLEQMPKPV
                     DDVKKPEVNITQIVAKYLKKIQPFNFNLSLNNSITNQYRTGTPDWQYRMGLTRDPGIP
                     FVMEEYIPTSAENTSNSFDFSVRSGINLFSNVVIPLSFAQNVSNTQSQGTVSRTLTRD
                     YIPSGINGKDGFPFPSWSLTWSQLEKYKIFSKYFKKLSLDHGFSGKESIITQNGKEMS
                     SSYKRYFQPLIGLSMQFVNDISASARVTSGQSINNASGGTTIIDDAGASISLSWQKKG
                     GLSIPLPFMKDKQLKNNITFTANFDYTNSTTRAKQGSATKFAVTAKSNSWKTTPRINY
                     TFTDKVTGGLFFEYGESYNRLTGKRVTRNYGFDVNIAIRG"
     assembly_gap    15171..15270
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15271..18581)
                     /gene="sprA"
                     /locus_tag="PKX09_07050"
     CDS             complement(<15271..18581)
                     /gene="sprA"
                     /locus_tag="PKX09_07050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOU17614.1"
                     /translation="MGFFVQSFAADREPDVFPLKIDWLSNRYTPANIESKRGLSLISP
                     DYATLNQNILKVQFPGMKVTNTLDKSGQHFIFYNDLNGAPVLTPIGIPVASYFDLRQR
                     LNWHNKLVEVVTAKPADTKAASDKLELIGADIAGQHVALRVSGNININGRLQTQKRSQ
                     VSSSYAEGTSTSFIVDQKQQLNIEGKIGDRISILVDQDSERDFDFENAMKIIYTGKED
                     EIVQKVEAGNVALSLPGTQFVTFSGKNNGLFGLKALMKFGGVDVTAIASVEKGKKEKL
                     SIDGGAQTSNISIKEYEYRRNLYFFLDTLFRDNMYRGYQYDQLFHADPRGKVTDLQVY
                     KSQTIETAGCAYGTAYVDPRDTTLYKDYSENRIFKLLELNKDYYVDVTLGYIRMTTQV
                     QDGEILAVAYRLTDDNNVTVTGEEYGDYNRSDTSTAHIILKLIKPQEMLPTHPCWNLE
                     FKNIYYLGTTGINSDGFELDIVYTLGEGEQNYDLVTGKKFLEMFGLDRVDNNGNPTPD
                     GVIDMNNGAILNLYSGELWFPYLRPFQYDSTDNTGKRNPNISKIYNCSAMYDSNRTDN
                     DILSETKFKIMVSYKNRSSILNLGAMVIENSETVTLGNSTLERGKDYIIDYFSGTLTL
                     LTSDATDPNAKLEVKYEKNQFFQLDKKTILGARAQYDFGENSYIGATALYFGQSVSDE
                     KVEVGYEPMHNFVWDINGRYNRNLDFLTRAVNWLPLVSTNAASSFDIEGEVAQVLPNP
                     NTLSNEETGDRHGVGFIDDFESSKRVTSPPIMRRYWSNAACPLGKDENERGFTFWFNP
                     YGGVPTKSIWPDKEVSTTAQNNITEIMVMSVDPNWSIAVVDGVSPVQSAWGGITYAFP
                     QSYYDQSTTKYVDIWVKGNSGTLHIDLGQISEDQVPNKKLDTEDIPDAGLTMGNGLLD
                     PGEDTGIDGLVDKNEKIVTTKYGELSYGDTRLTEYKRDISDPHSDNWKYTEGSKEYRY
                     INGTENNEKQTDGLFPNSEDLNNNYAIDLTNDYFTAAFDLSDPNNEYIAGKTYIDSRP
                     TGWKLYQIPLTAFSKSATGKDISWESIRACRIWFDGLTGEDSVFIAKVELVGNEWEDL
                     GIAKDDTSIFVVNENS"
     gene            complement(18716..19324)
                     /gene="rdgB"
                     /locus_tag="PKX09_07055"
     CDS             complement(18716..19324)
                     /gene="rdgB"
                     /locus_tag="PKX09_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004092423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOU17615.1"
                     /translation="MDILIATHNRDKVREIKRIFSLPELIWHTLDEYPDCPKVVEDGN
                     TLFENAFKKARMIAEFTGLPTIADDTGLEVDALHGQPGVFSARFAGENATYAQNVAKL
                     LEVMRDIPDSERQAHFRCVAVYYSPDLTLVEEGKIDGIILRQPRGNGGFGYDPVFYLP
                     QLGRTFAEMDLSEKNSLSHRGQAFTRLANKLKSVDKFVTDNI"
     gene            complement(19589..20152)
                     /locus_tag="PKX09_07060"
     CDS             complement(19589..20152)
                     /locus_tag="PKX09_07060"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HOU17616.1"
                     /translation="MIRCPWPANDELMIHYHDTEWGVPLHNDQKLFEFIVLDSFQAGL
                     SWRTILHKRENFRRALAGFEVNQVANFGEIEINSLLANVGIIRNRAKIQATINNAARF
                     LEVQQEFGSFDRYIWQFTAGKTIVNHWQKLSELPDKSLAAEAMSADLIRRGFRFVGPK
                     ICYAFMQAAGMVNDHLESCFRWQELQD"
     gene            20510..21379
                     /locus_tag="PKX09_07065"
     CDS             20510..21379
                     /locus_tag="PKX09_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOU17617.1"
                     /translation="MDIVALARKKGLLFDGAFGSMLIQHGLPTNANAEVWCLEHPEVV
                     TKIHAEYVAAGADVLTTNTFGASSLKLQKAGVEKRVAEINHRMVEMARAAAKPNTLIA
                     GNIGPTGEMLAPFGKISIEEATINYAEQAGFLDKAGVDLFIIETMFDLNEALAAIKGI
                     LQVSAKPIFATMTFSESPTGFVTIMGNPVEESMRQMVEAGAMAVGANCSIGSEKMVSL
                     AQIIRAAVSVPVVIQPNAGIPILRNGRLHYPEEVAFYTRNIMTIKQAGVEIVGGCCGT
                     TPEYIRAIRQALT"
     gene            21633..22283
                     /locus_tag="PKX09_07070"
     CDS             21633..22283
                     /locus_tag="PKX09_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOU17618.1"
                     /translation="MFLKESDQKFVREVFSRLNNPVKILNFTQTLECQYCRETRELLE
                     EITALSDKLSLEVYNFQIDRSVAERYGVDKIPATILFAETAPHSRIKFYGIPAGYEFT
                     ALLEDIVDISNNKTDLSPEILAGLKKVTWPVHIQVFSTPTCPYCPVAVRTAHRFALLN
                     ENIRADMIEAIEFPHLAQKYNVRGVPRSVINENIIIDGAVSEKQFLEKILSSQPTT"
     gene            22341..22931
                     /locus_tag="PKX09_07075"
     CDS             22341..22931
                     /locus_tag="PKX09_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOU17619.1"
                     /translation="MENNLPEEELVARAKKGDQAALSQLVLNYSSRIYHLGLRLTGSH
                     QDAEDILQDTFLIMVKKINQFQGKSSFYTWLYRIAMNVGLRKLKSKPRKYEHISLDDP
                     DFERLSSEAASEWPDFDYDIVNDPHFRQRLDRLIEQLPEKYRTVFILRDLHGLSTEDT
                     SRILQITPSNTKIRLMRARNFLKENLEKMLKTEMQL"
     gene            22928..23179
                     /locus_tag="PKX09_07080"
     CDS             22928..23179
                     /locus_tag="PKX09_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17620.1"
                     /translation="MKLDEELLKEICEYMGSDLNSAPCKIIRDHMEACPNCEVYIDKI
                     KRTIEIYRLADQCEEIPEKVTRNLLACLKLDDPSKTCKK"
BASE COUNT         6536 a         5044 c         5005 g         6640 t
ORIGIN      
        1 tcgccactca gcgccattca gttgcccaac aattccgcct ggccggttat gaaccgcaca
       61 aatgggaacc agccgaatgt ctcgccattt tacagttatt taaatgggct tatttaagcc
      121 aatggaatga aaaaatcgtg atttacaaaa ccggcgaagt gttcggggat gagcggactc
      181 gcgacggatt tcccatcctt gagcaaagtt ctgctatccg cgaatcgggt taccaaaaca
      241 ccttttttgc cagccttaat cagctgtatt gggatggggt taatttgcgc caggctatga
      301 accttttccc gaataaagat aaatcgctcg cctggatatt ggcaggtaaa aatgttcccg
      361 ctgaccagac tcgactaatt tttgaaaata attggtttaa ctcttacgat ttcattttcg
      421 atatggcggt tctcgaccgg cgaatggtcg gtttgttttt cccgggcgtc ccgatttgcc
      481 ttgcaggctg taatcagcag gtagcatttg ggactaactg gttgcccgaa aatgaggtca
      541 atttgttggt agttcggatc aatcccgcca gtgaacaata tttagcgcaa gacggctggc
      601 acaatcttcg aaagcgtagc acgcagattg ccgtccgcag tgaccataag caggaggtta
      661 cattttatca tactgatcag ggtgtaattc tcgattatcc aatcgagtcg gttgatacgg
      721 ttgcgtacgc tatcgtcccg aaatggcagg cggttactgc ctcagaaata aatcaacgag
      781 tagcattaat gttaattggt aataacgacg ccgcaagagt tatgactgaa gtcagttcca
      841 atctgccggg tcttgtttat cttgaccaaa aaggtaacta tggttcctcc gctttaccca
      901 aaccgctgac gcctaatctt agcattaatt gcttgacgcc cttcacgatt gacgattggc
      961 gtttttcatc tcaattcacg atagattcac tgaaaagtca ttttttaccg gattggcaag
     1021 accaacctaa agcgttattt tctgaatcca ctgacgaatt ttttccaatc ctcgctaccg
     1081 ataaatattt cacaagattg atcaaagagg tacgcgccgc cctaccagat acgattttta
     1141 cgcgcaagac ggaactccaa gcctatcaga atctaattaa gtgggatggt cgctatgcta
     1201 aaaccgccgt cggtccaacg atttatcagg cttttctgaa agcgctaatg cgaaatattt
     1261 acagcgacga actaaattta gttgattcgg aaacgtttga gcaatttgcc aattccggtg
     1321 attttgccgt gcaaaatttg ctggtattaa tcgagaaagg cgagagcaat tggtttgata
     1381 atctacgaac atctgatttt gttgaatggc agggagaaat tattcgtcaa tcatttaagg
     1441 aagcagtgca gttgatcgaa gaaaaatact cgccgaattt atccgaatgg caatggggac
     1501 gagtgaatca ggatgtcgta ttgaacaaca acatcaaaag aaataaactt tcaaatagca
     1561 aaagtgtgag tcttaactcc tccggcgcaa tccaaactat aatcttaaaa cctgccggaa
     1621 gcgaaacggc ttgttgcatt gcagtcaacg gtacaacgaa tccagtagaa ccagtcaatt
     1681 tgttgagcga tcgagccgaa ctattgcgaa atggtgctta tattatgact ttaaaagcga
     1741 gtccaaaaaa gtaaggagag taatttgctt gaggtaatca aatctaccat aatgataacc
     1801 ggtttcgttt tcactatgat gctggtaatc gaatatttaa acgttcagac tcggggaaaa
     1861 tggcacaaat tactaaacgg acgaaagtgg cggcaatatg ttctgggagc gttgcttggc
     1921 ataattccag gctgtctcgg ctcatttgcg atggtcacgc tttacactca ccgcttagta
     1981 tccataggca ccgtagtaac aacgatgatc gcaaccagcg gcgacgaggc ttatgtcatg
     2041 tttgccctct tccccggcaa agctttgtta ttgaatgtaa ttttagccat tatttcaatc
     2101 ccgattgggg tgttagccga tcttttattt cgcaaaaaat atgatgtaat aaccgcccat
     2161 gatttgccga tacacgatga atattgcgag tgctttcagc ggggaaagat tttagaacaa
     2221 ttacgctcta ttactctgga gagatttctg ttaattttaa ttatggcggc gattttgcta
     2281 gccgccgcga gcggtttcat tgccggagag gaagaaggct gggtgcgaat tacgttaatt
     2341 attacttcga tagtcgggct ggcgattgtt gcgacggttc ctgaccactt tctgaaggaa
     2401 catctctggg aacacgttac taaacggcat gtgcccagaa tttttttctg gacactcgga
     2461 gctttgatag tgatccagat tttgaccaat tacctaaata tcgagcactt tttgcaattg
     2521 caacaggcac gcatcctggt tttactaatc gcctgtttgg ttggaataat tcccgaatcg
     2581 ggaccgcatt taattttcgt gacaatgttc gccgaagggc tggtgccatt tagtatttta
     2641 cttgccagtt cgattgtcca ggacggtcat ggtatgttgc cgatgctggc gcattcgcgg
     2701 cgtgattttg ttcgcatcaa ggctgtcaat tttgggtttg gtctattagt cggtttgatt
     2761 gggctgtggc ttggttttta atccattatt caaaacgtca ggaataagaa attgaataaa
     2821 gcatttactt tccttgatct aacccatacc ctggaaaacg gtatgccgac ctttccgggc
     2881 gaccgtccta agccgcgaat tattacgcgc agtaagacac aattcacgat ttcagaaatt
     2941 catttaggta ctcattatgg aacgcatatt gacgctccgc gccatttttt gccagacgga
     3001 aaaagcatcg ctgattttaa tgtggaccgc tttaccggcg cggcgcttgg cttgcatcgt
     3061 gattcggctg gcacatccgc tctgaatcta aatgataaag aaattgagtt aattcaaaac
     3121 ctagaaccac aatggctgat tctgcgcact ggatttgacc ggttttggag tcagccgaat
     3181 tattttttag aacatcccta tctatcagtc gaattcgctc agaaaataat tgatctgaaa
     3241 attaaaggaa tcggcggcga tttcccgagt atggatgccg ctaatgccgc caggcaaaat
     3301 tttccaattc accatctctt aatggaacat gaagtattaa ttatcgagaa tctgaccaat
     3361 cttagcgcgc ttccaacgca ggaacttttt cacttgattg ctctgccgct caagattgct
     3421 accgatggcg cccccgcccg ggtagtagca tggttttaga cagaattgac cggatttagg
     3481 tttattttta gcagaattag cctgacaaaa tatttaagga aatattttca atgaaagaag
     3541 tagttatcat tggaaaaatc ctggagattt aattggattc ctttgttgaa ctatagcaga
     3601 atcttttata aaaatactgt caagcctgtt tactatcctt ccgactcaga cgcacgattt
     3661 catttcgcat ttgtattaga aattcgaggt ttttcgtcca ataataatcg gggtaagtcc
     3721 agggtagtat tttgaattgg ccatgttcgt atattagcgt tacttccgcg aagacaccat
     3781 caccgcaata gattcgatgt gagaaatttt tagtcgagaa taaaactaat ttcgatggtt
     3841 cgaggtagcc cgggtcaaga ttcacttgcc gacgggattg actgccggaa ttatctacaa
     3901 attgggattc gattcgattg gtccagactt taatttggtg agcattttcc aggctggtag
     3961 gttgtttgaa aatgaaaaat tgcttcttta aatcccgccc gaattccgat tcataataat
     4021 tggtgaagtc gctgacggaa aaaagcggac cggcgcccat tatttcacca aaacgagtcc
     4081 gcacctcctc cagtgcgact tggcgttgat gctcactcgc agacattatt gctaaaaata
     4141 aatgaccttg cggtgcggat ttaatctgtc ccatcacttg cctcattaga aatttggtta
     4201 ttgcctcgat tttcggcatt gcgttcattg gctacttggc gcagagctaa gtaatcttca
     4261 tagctgagat tgcgggaaag gaacggaaaa atcgtggcgt tggtcatcag cggatcctga
     4321 tcgtcgctaa cataaccaag cccacgcgcg cgctcccatt ctatcgtgcc gggaatcggc
     4381 gaaaaagatg ccagttttac aatcacaccc tgatcggcga cgaaattaat actatcaata
     4441 atctgctggc gagtttgacc gggtaagccc ataatcaagt aagtttccag tgaattgcgc
     4501 gcaaatccgg ctttagccag gttattgaca gcgtccgtgt aattcgcatt attgacttta
     4561 ttgctggagg atttttggat ttgagacgaa gtagattcca gacttaagcg cacagttttg
     4621 aaaccggcag agaacattag ttcggctaat tcagaattga tctcacgggc aaaaaggcca
     4681 tttggtgaat gcaggttaat ttttactccg gcagaaatta tcccctttaa tattggaata
     4741 atgtggcgat cgcgatgggc gaaaagagcg tcatcgtaaa aagcgaaatt ctctacgtta
     4801 aactggttgt aagtcgaaat aatttcgcta ataacatcct ccggacggcg ctgaacgaac
     4861 tttttattca aaaggtaagt ggcgcagtaa gtacagcgat tagggcaacc gcgtgaagtc
     4921 agaactgcca ctgactttag ctttggatat aaatgccagg gcaagatgcc gttatcattg
     4981 taatccggta tatcaccgta gtcccgttca atcccaaaaa gatgatcaat cagtctcaga
     5041 gctttctttt caccataacc ggttattaaa aaatcgggct ggattgaatt tcgggcatgc
     5101 tctggatata aactagcata aattccacca agaatcaaag gcgtttcagg aaaatagcgg
     5161 cgcaggattt cgaccgtggc ggcgaccccg ggataccagt aagcaatcaa cgacgttact
     5221 aggatggcgt tcggtcgcgc catattggag agagtgtcac tgaactgttc ccgactagcg
     5281 ccataaaggc caaaacgtcg cgggtagcgc gccagcaccg gcggtttggg aatgacggtt
     5341 ttgcgatatt tgccccgtcc gtcattgaac ttatcattac tggtgtcttg ccagcgttga
     5401 cggtccatta aatcgagtaa tacgatttca taaccgtagt tttgtagaaa tttggcaata
     5461 tacagcaagc ccagcggctt tgaccacaaa tcaaaagcgg taaaatcggc gatccaggga
     5521 ttaattagta ataaacttct atcagacata attaaaatct attgaatttc catgctgata
     5581 aaaagtgttt aatacatccg gtcagttctg aggatatttt aacgagtgaa ctcactacaa
     5641 gatgaatgct tacctcgaat agaaatagtc aaaaaaacca aagtggagtc cgggttagta
     5701 agctgatata ttttgatagc tggttggctt atttcgagaa tagtaagctt atagttctgg
     5761 tcggtagcta agagtaagga gattgggata acccagccca tccggcataa aaataaaggc
     5821 ataatcaatt tcgaaccgcc cgaaatttag accggccccc agggcagtcc ggttgattgt
     5881 cccgacccgt tcgtagccgc ctcgaatttt gattaaatcc gtgaatgcta tctctccgcc
     5941 gacctgaaag taggtttttt cagttaccaa attagcgatt aaatttatcc ccagattaac
     6001 attaaaaggg ctgtcggcaa aaatttccgg ccgtacgatt cctattttga aaagagtcgg
     6061 caaagtcgta gccttatctt tcagcgtttg catctggccg atgttactta gcgataatgc
     6121 taaatccatc ccgcttggaa tttgccagtg agtagcaatg tcaaacgcta ccccgccggc
     6181 attttcggca tagagatgtt cgtagagata ttttacggtg caacctatag ataatttcga
     6241 aaaaattgga taagcatatc cgacggcggc tgacatatat tgcgcttcga cctcacctaa
     6301 cgattcgtcg gagggaatgt ctcgaatttc gatacctgga atcttgacga aattaagccc
     6361 gaatccgaga ttgccttttt tgagaggaat cgtggccgcc agagcgtcgc cgctgacatc
     6421 ggcaaaccag aaattatggc tgaagccgat cctaatgtcg tcatttataa ttccaatcgg
     6481 gttatcgaaa atggtaaatg gcgtgccgct gacttgggca aaaacgccca tcgtcgaagc
     6541 agtttgagct gaaactggga tttttaaaaa cgccattcct gattcgccgc tttcggcctg
     6601 taaaaacatg ccggtgataa ttatgataaa tcctgttctt aatttttgtc gcatagtaac
     6661 catcaaaatt gaaattgcca tgaaaaaatg tgagtcgtgc cttcaccaac ggcacctatt
     6721 ataaaagaat aatctaattg agtagtcata ttagcaataa aaccatatgc cagacctgct
     6781 ccaaaagtcg gacttagttg gttcatcccg atccgcaaat atgccaggtt gcgataaaca
     6841 tattcggccc cgcaatgagt ttgccagaca ttctgatcgg agactccaac atctccgacg
     6901 aaggtcagct cctgtatggg tttaatagcc agaccaaatt tgagtatctg gggaaaattt
     6961 tctttataac tactgccttc ttcaccaaat aaatcctgtg tattccagtt ataaccggca
     7021 ccgatgtctt tgaattgcaa gccagccgta agccattcca atggctgata aagaacacca
     7081 aaatcgaatc cgagtccggt agcgctgacg tcgagcattt gattatgtaa aattttaaaa
     7141 ttaataccga gagagattcg gggatgaaaa gcgttggcaa aggaaatcaa gaccacgttc
     7201 tcgccggttt ggtaagtttc atcaacctga ccaatggaat tgcgaccttc gatgtccttt
     7261 actccagcat gaagccagga tactcccaca ccggcatttg gcggaagcgg aaccgataat
     7321 ccgataaaat gaaactggcg atcaagactc aaaaaagaat aggaaagcgc caagtttctt
     7381 tcatccatat atgccaaacc agctggatta tagtaaattc caaaaccgtt agatggaaag
     7441 gcaactcccg aattacccat cgctgttgag cgagctccga gacccattcg cagaaatgcc
     7501 ccggcataac cagcattgtc attcgtttcg ccctgcaata gagccatcga aaagatgagc
     7561 gataatataa gattcggaag ctttctcata attaattgat cacgatcagt tttgtccagt
     7621 gctccacttc atttccattt gatttttgga tcagtttgca gaaataagta ccgttggcta
     7681 caagctcgcc attcgttgcc cgaccattcc agatcaaatg atgatctccg gcatccataa
     7741 ccatcttcca atcactgcgg taaacttgtt ccatcgcaaa attatagatt tccagtcgaa
     7801 ccgatgcggg ctgatctata ttatattgaa tgcgaacatg gccatcatca cctattacat
     7861 tttgataacg cggcgcaaac ggattgggat aggcataaat ggctggttga ccggggcttt
     7921 tggtcgaaac agccgaatga taaactgtcc aggtcaatcc atcgctagcg gttttggcga
     7981 cgccgtctcc ggtgccgacc cataaatagg ttggtccttc gcgtcgatca acttctgccg
     8041 cgtaaaaata ttgggttaaa atttgttcac cggaagcaat gtcggcaatc ggtgtataaa
     8101 tagcccaatt ttcaccatca attgacttaa acaagcctct ctccgtacag acatagacaa
     8161 tcgaatcctg aaaggcaaaa ttataagggc gttcattgac taaggttgtg gtccaagtca
     8221 acccaccgtc gctggtcttg ctaacagccc gatattctcc gctttctccg actgtcaacg
     8281 tggcggccca gatggtctct ttgcctttcc aaagttggcg gtcaattgct acaacgaaat
     8341 tgccggaaat tgcggaattc tgggcgttaa atttgcgcca gctgatgcac tcaggagatt
     8401 caactatgcc gagattaatt ccatcagctg tcccgatcca gatcgtatcg ccataagcca
     8461 gtatgctaaa acctttatga ttatgattgc catccggtgg atcgcgcgga ttaatctcga
     8521 agtctatcag atcatcacag tccaaattat taaggtcatc agacggtaaa ggaattcttt
     8581 gccaggtctg tcccatatca gcgctccgcc gaataccacc agcccagcta acgatataga
     8641 cgtaatcatc agttatagca atatcccagg tggtattatt gatcggtgtc gttaccggca
     8701 aaaaggaaac aatctgattg ttccattcgt cactgttatc agttgggtcg tcaatcggtt
     8761 gtgggacata agtccaattt tgccccttat cggtggaata tgatagaccg gcgccggttt
     8821 gttggtaacc gtaagattgg attaacgaat caaacacggc cgccacccag atcgtatcgc
     8881 ccttgaccgc tattgcggaa atgccgccgc ggggcaaatt attcaacccg ggataaaaac
     8941 ttacgaacgt ctggccgaaa tccgggctca tactcaaacc gccgcccgtc gcaaaccaca
     9001 tcaaggaatc gccctgactg acaatttccg ttacactgtt gcttgccaga cccaaaaagg
     9061 tgttgtcact atccagggtt ggtttagcca gacgaaaagc ctgactaaaa ccggaggtga
     9121 agagaaatcc tattatgaat aaaatcgccg gatatttttt cattgggtaa tcaccagata
     9181 atcatcattg acatcataac gcgccggttt ggtaaggtcg gaaacgcgca ctttacagta
     9241 atcggtaacc tgagtagccg gtacagtcca gtcataatac ttcaaagcgg ctgcggttgt
     9301 atcggcgaca atccagtccg gattggtctc attggcatta aaagtgtact cgatttttac
     9361 ctgactaata taggcatact cccacataat tcttagagtt tgtccgaagc acagagtcag
     9421 ggcactggct ggggctgtaa tatgaatccc cggactggtg atttttaatg ttatggtatc
     9481 gctccttgcg ccggagtaat cttcagcgaa gaaataaaac gtatataaac cttgatactt
     9541 actctcagga tcagaaggct gaaaaaccgt gaaaatgcca tccctagctt tttcatcacc
     9601 atattctcct gcatcattta aataaaagta cggatcaaaa aattnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnttttca tcaccatatt ctcctgcatc atttaaataa
     9781 aagtacggat caaaaaattt tccgtcggga cgcaggatcg aataatagac agctactata
     9841 tcgtcatagc cttgtaaatc ggttactctg aaataaaatt tcagtgtatc ccacttttcg
     9901 aaagtcgtcg gtgctgaaag cagaaagatt tccggtggac tattggcttg cacgagttca
     9961 gtcgcggtgg ctgaatcgct gacattacca tctgcatcgt atgcccaaac aaagacccgg
    10021 taatatcctt cataacttcc caattcggtc gaatctatta aataggaaaa aacgccgtct
    10081 cgcgtaataa tatcgcgcgg cggaccgtca tctgacaatt ttccactttt aaaaaggaaa
    10141 ccgttcatcg agtctatcgt attaattcga tatagattga aaactaccga atcgagattt
    10201 tcccaacctt gatcgtcctc gactttggcg gcaataaaca ggctatgatt caattccgaa
    10261 taatgtaaag tcagattttc aataatcggc gtgggctcaa cggtttccac aattgactta
    10321 ttttcgcaag tccaaagcgt taaaatcgta ataaacaggg cggcaattat tattcttttc
    10381 atttacttaa tcaccaatgc cagccattca tcttcagtat cttgatcaag aatttcacgg
    10441 ccgtaattta agaccttatt agtaaaaatc tcattttctt ctttaagcag gccggtaaaa
    10501 atcaccagcg gacgatttcg ctggtgcaca agcgcctcca ataatcgcaa attggatagg
    10561 cgttcgatat tactaacaat cagatcgtag ttataatttg gcagggcggt tacatcgccg
    10621 atctgaatcg cgacctttcg agagagccca ttcaactcca aattttcatt gaaattatca
    10681 atgaccagcg agtcaatgtc ccaagcgcta acctgagccg cgcccagctt caacgccgca
    10741 attgtcaaaa tgccgcttcc gcaaccggca tccaatagtg aattgcctgg tgtcaagtat
    10801 ttttccagca ttaacagggc taagtgcgta gttggatgag ttccggtacc aaatgccatt
    10861 ccgggcttta gtacaatttc taccggcacg tctttaagcg gtttttccca gtaaggacgt
    10921 acagcgatgc gtggagtgat aatctgcggt ttgaaatatt cctgccacct ggtgctccaa
    10981 ccattgtcaa cgatttcagt gatggtgata ttaagttgag gccattcagc cgcaatgagc
    11041 tccggcaacg acaatttcat atttagggga aaatagattt catagtccgt atcggtttcc
    11101 tgaagtccga gcatacccca ttggatcagc cgggcaatta tgagttcttc atttccggcg
    11161 cttttgggga taagcacttt aagccagcgt tgataagtcg caatcatctt cgctaccttt
    11221 cttcagaacc gtaaaaaatg tattcgtaaa ggactgtgcc gacgatttcc aaactttccg
    11281 cactacactt atcgggtgtg tcgagcgtcg tgtgccagta attgataaac cggttaggat
    11341 actgaaaatc aattaaatca accgacggaa tcccggcgtg ttgaatcaac attaggtggt
    11401 catcgtaaat ttcctcatca atttgatgaa cgaaggcgct gaagttatgc cgatcggcaa
    11461 tagcccagag ttttttgatt aattggggat ggctcttgac cgaatttcgc tcgaccggga
    11521 tccgcaaatc agcgtcgcca atcatatcaa tgataatagc ttcttcgggt ttgggaatcg
    11581 gcaagttttt ggcaaaatat tccgagccga ggcagtaata atccagtgta ccaggaaatc
    11641 cgtaatcttc accgtcaaaa aacaccagat aaatagtgcg gggcggttgc tgtcgagata
    11701 gaatctccgc cagatgcatc aagactgcta cgccactggc gccatcatta gcgccgataa
    11761 tcggagtttc gcgtttagct ggatcggggt catactcggc gcgaggtcgg gtatcccaat
    11821 gggcaccaat caacaagcca ttagtgcttc gaatattaaa tcctgcgatg atatttgtca
    11881 gatttaaaat cttattttcc tcaggaatca agtactcgaa atcctgaatg atcaaagtat
    11941 cagccagcgg tttcaaaaac tctatcagca ttgtgcgctg agcagaatgc cctggcgatc
    12001 cgggatttcg cggtcctaaa tcacactgct gattcaaaaa ctgaaaggct cttccaccat
    12061 caaatttgcc accgccggat gagcatgctg aaatcaaaat taatattaaa taaaaacttt
    12121 ttttcactat taacctcgaa ttgctatatt gacgtcgaaa ccgtaattgc gggtgacgcg
    12181 cttgccggtt aacctattgt aactttcgcc atactcgaaa aacagaccgc cagtaacttt
    12241 gtcggtaaaa gtataattga tccgtggagt agttttccaa ctattacttt tggcggtgac
    12301 ggcaaattta gtagcggagc cctgtttggc acgagttgtc gagttggtat aatcgaaatt
    12361 ggctgtaaaa gtgatgttgt ttttaagctg tttatccttc ataaagggta aaggaatgga
    12421 taagccgcct tttttttgcc agctcaggct gatggaagcg ccggcatcat ctatgatagt
    12481 agtaccaccg gacgcattat tgatagattg accgctggtg acccgggcgc tggcactgat
    12541 gtcattgacg aattgcatag aaagcccaat cagcggctga aagtaccttt tataagacga
    12601 gctcatctct ttgccatttt gagtaatgat agactccttg ccagaaaagc cgtggtcgag
    12661 agaaagtttc ttaaagtact tggaaaaaat tttgtatttt tccagttgac tccaggtcaa
    12721 cgaccagctg ggaaatggga agccatcctt tccattgatg ccggacggaa tatagtcacg
    12781 ggtcaacgtg cgacttaccg taccttgaga ttgggtattc gagacatttt gcgcaaaact
    12841 cagcggaata actacgtttg agaacaaatt tataccggag cgaaccgaga aatcgaagct
    12901 gttgctggta ttttctgcac tggtgggaat atattcttcc atcacaaatg gtatcccagg
    12961 atcgcgcgtt agacccattc gatattgcca atcgggagta cccgttcgat actgattggt
    13021 aattgaatta tttagcgaaa gattgaaatt aaaaggctgg atctttttca gatacttggc
    13081 aacgatctgg gttatattta cttctggttt cttgacatcg tcaactggct taggcatttg
    13141 ttccagtgtc tgtcctgaag taggttttaa atcttctcct ttatcaatgg tcgatcctgg
    13201 ttttcgcggt cgtttttcac cggatttttg atcagtccgg gttgagtcgc ctgtaatcgg
    13261 cgccggttcg gctgttttgg gctgattctg ggcaccagtt tgcgttgctc gacgcctacc
    13321 ggtagtttga ccgggaggag taggggttgg agatggagcc ggggcagtac cactaggttt
    13381 aaacaatgat gaaaatattg ccgatggatc gaggctaaag gtactagtca aacggttctg
    13441 attagagatg gcatcaacac ttgcggaatt actgacaata ggctcattcc agttaaaatt
    13501 ggacgtatag ttaaaggtag gattgagcca tttggcgatt ttgggattaa aattgaccga
    13561 ataagactct ctcatattag taatctgccc cggattggca gattttagaa tttcccattg
    13621 attctgccat aattcgatca ggttattttc aagatttaat gtatagttgg cgctaaggtt
    13681 atcaaatatt ttataaccta acgataaatt gcggttgagt ccaaagctat tgctggaagt
    13741 tatagttgtg ccatagcgag gagtactata tgattcactt cggccggcat ccgccgcgac
    13801 tgtcaaggcg ttcgggaaat aataaaagcg gatttcgctg agtttatttc cgataagcgg
    13861 caccggagct gtccacttga aaatatttat atatttatct tttggaaagg gaaaattata
    13921 ggaaatgctg gctttgtacg actcactttg cgattcgcgg acggttaccg agctggcatc
    13981 ggtccaggta gcgttagcac tgatttttat ctggtctatg gtatatttag ttagccagaa
    14041 gtccgaagcc cgcttttcaa tcgaggtgtt tactccgtaa ctatgcgaga tgtccagcag
    14101 gctatcggga gcattttctt tcatcagaat atcactgccg tagatatatt tgcgcgttcc
    14161 ggtggtttgg gtatatgagc cgctaaccgg gagtcgtaag ccccagctgg ccggcgtgaa
    14221 tttgtccaga gaaatacgtc cggtggctgt caaggcttcg gtggttttca aattttccgt
    14281 ctggaggctg ggacgctgtt caaccgtatg aaaatccgca tctctatagt taaccgaaac
    14341 gtcaaagctg gctaaatcag ctacgcgcag gctggctttg gcgcggtagg ctatgccctt
    14401 gtccttgcga acctccgata ggcgcaactc atccagccag acctgacctt tgatagttcc
    14461 ggttttaaat ttattgacat atccgctatt atcagcccgg ttgcgcaaac cgatattaag
    14521 gtgtttaacg tttgccaacg agggtttgcc ataaattatt atttctcgac cggtataagc
    14581 atcgtttaat ttttctttat aatggcgaat cgtaacaccc gaagtgttag tatatatctg
    14641 gtattcacct tcgccgatgt agtcatctgc cgactcgtaa tttttcaatg aggttagaaa
    14701 atcgagatcc agttccatct cattttcctc agcccagccg ggatagatcg gttggcgata
    14761 ttcataatag ttcgttgtcg tcggcgaatt ttcgaatatt ttaccaaacc ggataaagaa
    14821 ttgaagtggg gtttcttcgc cttctgagaa ttgataacca tcggctttta gttcatgccc
    14881 gtttacgaac aatttcatta ctttataagt gatgtagcta gtttcttcgg caagcacttt
    14941 tttggcggcg caaaattcgc cggggaataa accagaaccc tcttcaggta aattcaaaac
    15001 cagagcttgt tctttggtac gaatctggtt gatttcatca tattcaccct gaacaccggc
    15061 tggtggctca taaatgtcag gattatcttc agtgttaatg actgaaatcg aaaaggaatt
    15121 ttcattgaca acgaagatgg acgtatcgtc tttagcaatt ccgaggtctt nnnnnnnnnn
    15181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gaattttcat tgacaacgaa gatggacgta
    15301 tcgtctttag caattccgag gtcttcccat tcattaccca ccaattctac tttagctatg
    15361 aatacagaat cttcgccggt taagccatca aaccagatac gacaggctcg gatgctttcc
    15421 cagctaatat cttttcccgt agcgcttttg gaaaaggcgg tcagcggaat ttgatagagt
    15481 ttccagccgg tgggtctgga atctatataa gtctttccgg caatatactc attattaggg
    15541 tcggacagat caaaagcggc ggtaaaataa tcattcgtca ggtctatggc ataattgttg
    15601 ttcaggtctt ccgaattggg aaaaaggccg tcggtttgtt tttcattgtt ttcggtgccg
    15661 ttgatatagc gatattcttt ggaaccttcc gtatatttcc agttatcaga atgaggatca
    15721 gatatatcac gtttgtattc ggttagcctg gtatcgccat aggagagttc accgtacttc
    15781 gtggtcacaa ttttttcgtt tttatcaacc agcccgtcaa taccagtgtc ttcccctggg
    15841 tctagtaatc cgttgcccat tgtcaatccg gcatccggga tatcttcggt atcaagtttt
    15901 ttattgggga cttgatcttc actgatttga cctaaatcaa tatgaagtgt gccagaattg
    15961 ccttttaccc aaatgtcaac atatttggtc gtagattgat cataataact ctggggaaag
    16021 gcgtaggtaa tgccgcccca ggcagattga acgggagata ctccatccac taccgctatt
    16081 gaccaattcg ggtcaacact cattaccata atttcagtaa tattattttg ggcggttgta
    16141 cttacttctt tatcaggcca gatacttttg gttgggacgc ctccataagg attaaaccaa
    16201 aaagtaaaac cgcgttcatt ctcatctttg ccaaggggac aagccgcgtt tgaccaataa
    16261 cggcgcataa tcggcggaga cgtcactcgt ttggaacttt caaagtcatc tataaaaccg
    16321 acgccgtggc gatcaccagt ttcttcgtta ctgagagtat ttgggttagg caagacctgt
    16381 gcaacctcgc cctcgatatc aaaggatgaa gccgcattgg tgctgactaa tggcaaccag
    16441 ttaaccgctc gcgttagaaa atccagatta cgattatagc gtccattgat atcccataca
    16501 aaattgtgca tcggttcgta accgacttcg actttttcat cactcactga ttgcccgaaa
    16561 taaagagcag tggcaccgat gtagctgttt tcaccaaaat cgtattgtgc tctggcacca
    16621 aggatcgttt ttttgtccaa ttggaaaaac tgattctttt catattttac ttctaatttc
    16681 gcatttgggt cggttgcatc tgatgttaga agagttaacg ttcctgaaaa ataatctata
    16741 atgtaatctt tcccccgctc cagagtactg tttccaaggg ttactgtttc actattttcg
    16801 ataaccatag ccccaagatt gagaatcgaa ctgcgatttt tatagcttac cataatcttg
    16861 aatttagttt cgcttaaaat gtcattatcg gttcggttgc tatcgtacat tgccgaacag
    16921 ttatatattt tgctaatatt aggattgcgt ttgccggtgt tatctgtcga gtcgtactga
    16981 aatgggcgta aatagggaaa ccataattca ccggaataca agtttaggat cgctccatta
    17041 ttcatatcaa taacaccatc gggagtagga ttaccgttat tgtcaacccg atctagtccg
    17101 aacatctcca ggaatttctt accggtcact aaatcgtaat tttgctcacc ctcacccaaa
    17161 gtataaacga tatccagttc aaatccgtca ctatttatgc cggttgtacc caaataatag
    17221 atatttttga attccagatt ccagcaggga tgagtgggaa gcatttcctg aggtttaatc
    17281 aatttcaaaa ttatatgagc ggtagaagta tcagagcgat tataatctcc atattcttcg
    17341 ccggtgacag ttacgttatt atcgtcagtc aagcggtagg caactgccag tatttcacca
    17401 tcctgtactt gagttgtcat gcgaatatat cccaaagtca catcgacata ataatcctta
    17461 ttcaattcca gtaatttaaa aatgcggttt tcgctataat ctttataaag agtagtatcg
    17521 cggggatcaa cataggcggt accataggca caaccggcag tttcgatcgt ctgagattta
    17581 tagacctgca aatccgttac tttccctctt ggatcagcgt gaaataattg atcatactga
    17641 taacccctat acatattatc gcgaaagaga gtgtcgagga aaaaataaag atttcggcga
    17701 tattcgtatt ctttgatgct aatgttcgag gtctgcgccc cgccatctat actcagtttt
    17761 tcttttttgc ctttctcgac gctggcgatt gcggtaacat caacgccgcc aaatttcatc
    17821 aaagctttaa ggccaaacaa gccattgttt ttgcccgaaa aagttacgaa ttgagtaccg
    17881 ggcaaggaca gagcgacatt accggcttcc actttttgaa caatttcatc ctctttgccg
    17941 gtataaataa tcttcatagc attttcaaaa tcaaagtcgc gctcactatc ctggtcaacc
    18001 aatatgctaa ttcggtcgcc aatcttaccc tcaatgttca attgctgttt ctgatcaacg
    18061 ataaaactcg tcgaagttcc ttccgcatag ctactgctca cctgtgaacg tttctgggtt
    18121 tgcagacgtc cgttaatatt aatattaccg gaaacgcgca aagccacgtg ctgaccggca
    18181 atatcggccc cgattagctc cagtttatca ctggcggctt tagtgtcagc cggtttggcg
    18241 gtaacaactt cgactaattt attatgccaa ttcaggcgct gtctgagatc aaaataacta
    18301 gcaactggaa ttccaatggg agtaaggact ggagcaccgt tgagatcatt gtaaaaaata
    18361 aagtgctggc cggatttatc gagggtatta gtcaccttca tcccgggaaa ttggactttg
    18421 aggatatttt ggttcaatgt cgcataatcg ggtgaaatca acgacaaacc gcgtttgctt
    18481 tcgatattag ctggcgtata gcgattagac agccagtcta tttttaaagg aaatacgtcc
    18541 ggctctctgt ccgcggcaaa actttggaca aagaacccca ttaaaataaa tatgggtaaa
    18601 gcatatcttt ttatcagatt gatattaatt tactaacctt ttaatatggt ggttatggtg
    18661 gttaaaccga cgcaaagctt attgcgcgca gggtagcaat tctaaaaaaa gtacctcaaa
    18721 tattgtcggt tacaaattta tcaacggatt ttaatttatt ggctaatcga gtgaacgcct
    18781 gaccgcgatg actaagggag ttcttttcgc ttaagtccat ttccgcaaag gttcgaccga
    18841 gttgtggtag ataaaatacc ggatcgtaac cgaacccgcc atttccgcgc ggttgacgta
    18901 atatgatccc gtcaattttc ccttcttcca ctaaagtcaa atccggactg tagtaaaccg
    18961 ctacacagcg gaaatgcgct tgtcgctcgg agtcaggtat atcccgcata acttccagca
    19021 atttggctac attttgagca tacgtagcat tctctcccgc aaaacgcgcc gaaaaaaccc
    19081 caggctgtcc gtgcaacgca tccacttcca agccggtgtc atcggcaatc gtcggtaatc
    19141 cggtaaattc agcgatcata cgtgctttct taaatgcatt ctcgaaaagc gtgttcccgt
    19201 cttcaacaac tttgggacag tcgggatatt catccagagt gtgccatatc agctccggta
    19261 gagaaaaaat tcgcttgatt tcgcgcactt tatcccgatt atgagtagca atcaaaatgt
    19321 ccatttacgt tctctaagca cttttaatta agcggaaaat gttatgcata aattaagtta
    19381 atgtcaagct ttgacaaaca aaaatctaaa taataaattt tctttcaaga taataaactt
    19441 gttttccaat tgaaaaatta ctcagatgct tgtttcggag tagaaaactt ctaaattgga
    19501 cgaaatttta tcattaaagc atataatttt acaattacta aattaacaaa tagttatagc
    19561 ggctctagtt atttcaccat tggattcttt agtcctgtaa ctcctgccaa cgaaaacaac
    19621 tctcgagatg atcgttgacc atccccgccg cttgcataaa tgcataacaa attttcggac
    19681 caacgaaccg aaagccacgc cgaatcagat cagcgctcat agcttcggct gccaagcttt
    19741 tgtccggtaa ttccgataat ttctgccagt ggtttacaat tgttttccca gcggtaaatt
    19801 gccagatata acgatcgaat gacccgaact cctgttgaac ttctaaaaag cgcgcggcat
    19861 tatttattgt cgcctggatt ttagcccgat tacgaataat tcccacattt gccagaagcg
    19921 aattgatttc tatttcaccg aaattagcaa cctgatttac ctcgaagcct gccagagctc
    19981 ggcggaaatt ttcgcgcttg tgcagaatcg tacgccagct tagcccagcc tgaaaactat
    20041 ccaatactat gaattcaaat aatttttggt cattatgaag cggtaccccc cattcggtat
    20101 cgtgataatg aatcatcagc tcatcattag ctggccaggg gcaacgtata atcttagtca
    20161 aattgaaatc tccatcttac tctactgtta aattcaaatt tataaaatta tttaggtatt
    20221 tagacaaaat tcacaatttt atggaaatca ccaaaaaata gatattcaaa aaaaacagga
    20281 cattaccctc gccttttaga gataaataag attagcaaaa gcttgttttc gaactcttgt
    20341 ttaccgctga ataagtccag taataagttt agaataacga gcaactttta tcattcccaa
    20401 aaaattagga gcaatcctgt taaggttggg aatgctatga ttttgcttct gtaccttttg
    20461 ctttctattg cgcggctttt tacattactt cacaattgga gaaagataaa tggatatagt
    20521 agcattggct agaaaaaagg gattattgtt tgatggagct ttcggctcca tgctgattca
    20581 gcatggtctg cctacgaacg cgaacgccga ggtctggtgt ctggagcatc cggaagtggt
    20641 aaccaaaatt cacgccgaat acgtcgcggc tggggcagac gttctcacaa ccaatacttt
    20701 tggggcgtca tcgttaaaat tacaaaaggc aggtgtcgag aaacgcgtcg ccgagattaa
    20761 ccatcgcatg gtagaaatgg cgcgcgccgc cgcaaagccg aatactttaa tcgccggtaa
    20821 cattgggcca accggtgaaa tgttagcccc ttttggaaaa atcagtattg aagaagccac
    20881 cattaattat gccgagcagg ctggttttct cgataaagcc ggggttgatt tattcataat
    20941 tgaaacaatg ttcgatttaa atgaagctct ggcggcaatt aaaggcattc tgcaagtgtc
    21001 tgccaaaccg atttttgcaa ctatgacttt tagcgaaagc ccgaccggtt tcgtaactat
    21061 tatgggcaat ccggtggaag aaagtatgcg ccagatggtc gaagccggcg ctatggcggt
    21121 cggtgccaat tgttcgatcg gcagtgaaaa aatggtcagc ctcgcccaaa taatccgcgc
    21181 ggctgtatcg gttccggtgg tcattcagcc gaatgccggt attccaattc tgcgaaatgg
    21241 cagattacac tatccggaag aagttgcttt ttatacccgc aatattatga ctatcaaaca
    21301 agccggtgtc gaaatcgtcg gcggttgttg cggtacaact cccgaatata ttcgcgctat
    21361 ccgccaggcg ctcacttaaa atttcagtcg aagattttca caataaatca ttttgattcc
    21421 gtagagaagt cggtaatttt tactgcattt tcactaataa cgatctggta aaattccggt
    21481 gtaattcaca aacaccaatt tgctgataaa tcaaatttgc caaagcttga ggctatttta
    21541 cgccaggtct aaaaatcata atgtaacctc gcctttgtaa ctctatctaa aaaatcacca
    21601 acaaataata caaatgaaaa aatggagtgg aaatgttttt aaaagaaagt gatcagaaat
    21661 tcgtgcgcga agttttcagt cgtctgaata atccggtcaa gatcttaaat tttacccaaa
    21721 ctctcgaatg tcaatactgc cgcgagactc gcgaattgct ggaagaaatc acggcacttt
    21781 ctgataaatt gagtctggaa gtttacaatt tccaaattga ccgttctgtg gcggaacgat
    21841 atggcgtgga caagattccg gctaccattt tgtttgctga aactgctccc cattctcgga
    21901 ttaaatttta tggaattcca gccggatacg agtttaccgc cctgctggaa gatattgttg
    21961 atatttctaa taataaaacc gacctatctc cggaaatctt agcaggccta aaaaaagtta
    22021 cttggccagt gcatattcag gtttttagca ccccgacttg cccatattgt ccggtggcgg
    22081 tgcgcactgc tcatcggttc gcgcttttga atgaaaacat tcgggctgat atgatcgaag
    22141 ccatcgaatt tccccatctt gcccaaaagt ataacgttcg cggtgtaccg cgctccgtta
    22201 taaatgaaaa tataatcatt gacggtgccg tctcagaaaa acaatttctg gaaaaaattt
    22261 tatccagcca gccgacgact tgattagcgc aattaaaata ccaatggaat taacgatgtt
    22321 attcattaaa tttaccatcg ttggaaaaca atctaccgga agaagaatta gttgctcgcg
    22381 ccaaaaaggg cgaccaagcg gctctgtctc agttggttct caattacagc agtcgcattt
    22441 atcatctcgg gttgcgttta actggtagtc atcaggatgc tgaggacatt ttacaggata
    22501 cgtttctaat tatggtgaaa aagatcaacc aatttcaggg caaatcatct ttctatactt
    22561 ggctatatcg catcgcgatg aatgttggct tgcgaaagtt aaagtccaaa ccacgcaaat
    22621 atgaacacat ttcgcttgac gatcccgatt ttgaacgatt aagttccgaa gccgccagcg
    22681 aatggcctga tttcgattat gatatcgtca atgatcctca ctttcgccaa aggttggatc
    22741 gtttgattga acaattaccc gaaaagtatc gcacagtttt cattctgcgt gatttacatg
    22801 gattatcaac cgaagacacc agccgtattt tgcagatcac accttccaac accaaaattc
    22861 ggctgatgcg ggctcgtaat ttcctgaaag aaaatttgga aaaaatgtta aaaaccgaaa
    22921 tgcagttatg aagctagacg aagaacttct caaggaaatc tgcgagtata tgggtagtga
    22981 tctaaactcc gctccctgta aaattatccg tgatcatatg gaagcctgcc cgaattgtga
    23041 agtttacatt gacaaaatta aaagaacgat tgaaatttac cgccttgccg accaatgtga
    23101 ggaaatcccg gaaaaagtca ctcgtaatct tctagcttgc ttaaaacttg acgatccaag
    23161 caagacctgt aaaaaataat ctcacccttt cttcagaata gattacaata gccaaaatca
    23221 aaaattttgg cgtgctttgc ttcttgacca cagaaacacc cgggcaaaaa agggctaagg
    23281 ctgagaaggg gcattggtca ttagtcatta gtcattagtc attggtcatt agtaaagagg
    23341 ctgagtaaag agattgagac taagaaaggg aggcaggaga cagtttcgcg gaaattggtt
    23401 tggctatttg tgatttggaa tttgg
//
LOCUS       DAOSMP010000036        21002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_2527_length_21002_cov_17.181553, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000036 DAOSMP010000000
VERSION     DAOSMP010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 21002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21002
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2527_length_21002_cov_17.181553"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PKX09_07085"
     CDS             <1..1137
                     /locus_tag="PKX09_07085"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOU17621.1"
                     /translation="PGWGSCSGLFTANSMNCLCEALGIALPGNGTILAEDERRIELYK
                     AAGARIVELVRSNLCPSQIITRASIDNAIVMDMAMGGSSNTVLHLMAVANEAGIDYSI
                     NRMDEISRKTPTICKVAPSSPYHIEDVDAAGGMSALLNEIFRENEFVNKDCLTVTGKT
                     IAENVAGKRILDDSVIRHLENPYSPTGGLAIMTGNLAPEGSVVKYAGVSDKMKVFEGP
                     AVIFESQEEACEGILSGKVKAGDVVVIRYEGPRGGPGMQEMLSPTSYIMGRGLGESVA
                     LITDGRFSGGTRGACIGHVAPEATVGGPIALLKNGDRIRIDIPQKSLEVLLSDSELQS
                     RRAQWQARPPRITHGVMARYAEMVASASQGAVLKYNSRIKGEKL"
     gene            1137..2873
                     /gene="ilvB"
                     /locus_tag="PKX09_07090"
     CDS             1137..2873
                     /gene="ilvB"
                     /locus_tag="PKX09_07090"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOU17622.1"
                     /translation="MKGAEILIDALINEGVDTIFGYPGGVVIPIFDVLYRTDKIRFIL
                     TRHEQGATHAADGYARATGKVGVCLVTSGPGATNTITGIANARMDSIPIVVISGQVDT
                     ANIGTDAFQETDMIGLTRSITKHNYLVKSVEELPIVLKEAFYIARTGRPGPVAIDIPV
                     NVSNAQLKNYQYPSEVTLPGYKPVTNGNPKQIEKLAQAIKTAKKPLLYTGGGIISANA
                     EPELLALLDKTNIPIVITLMGLGSIPSDYPTFLGMPGMHGTMAANLALNECDLLIGIG
                     VRFDDRITGKREQFARQATVAHIDIDPAEIGKVIKPHIPVVGDAKKILSELVKKVSPR
                     PKDEWNERTLAWKNQYPMTYDQDAEEAILPQYVIDRVSALADPDAILVTDVGQHQMWA
                     AQYYRHRKPRSFISSGGLGTMGFGLPAAMGAAVGRPGHTVITISGDGSIQMNIQELAT
                     CSINKIPVKVIILNNSYLGMVRQWQELFWDEHYSQTCLKRSPDCPPQCTGPRKDCPSV
                     YIPDFVKLAEANRIYGYRATKPEEVDDVLKQGLSQPGPVIMEFVVKSIENVYPMIPSG
                     KTISDVIPGRQS"
     gene            2870..3355
                     /gene="ilvN"
                     /locus_tag="PKX09_07095"
     CDS             2870..3355
                     /gene="ilvN"
                     /locus_tag="PKX09_07095"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOU17623.1"
                     /translation="MIEKHTIIVTVVNRVGILARITGLLAQRGYNIESAIAAPTEEKD
                     IYKIILVVKETSENIEQITKQLNKLIDTIKVTDISHKDNYIVREYIILKVGLTPRTRA
                     EVLDLMRVFGAKPIDLATDHLIMELAGHVRKIDRFIELLKPFGIEEYVRSGEFAMTEY
                     N"
     gene            3373..4374
                     /gene="ilvC"
                     /locus_tag="PKX09_07100"
     CDS             3373..4374
                     /gene="ilvC"
                     /locus_tag="PKX09_07100"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOU17624.1"
                     /translation="MSVKLFYEKDVKSDSLKKKTIAIIGYGSQGHAQAQNLRDSGYKV
                     IIAEMPGSPNYALAVKHGFKPVSAAEASQQADVIQILTPDEVQKRVYEQEIKQNLTKG
                     KALVFSHGFNIHFKQIVPPADVDVYMVAPKGPGHLVRRQYEIGAGVPSLFAVYQDVTG
                     QARNLAMEHACGIGAGRAGIIETTFREETETDLFGEQAVLCGGVSELIKAGFQTLVDA
                     GYQPEIAYFECCHELKLIVDLIYEGGLAGMYYSVSDTAEYGGMTRGPYIIDCETKKRM
                     QTLLRAIQTGSFAKDWILENQAGRPTLNAYRQEHQELLLEKVGRELREMMPWLKKKG"
     gene            4378..5958
                     /gene="cimA"
                     /locus_tag="PKX09_07105"
     CDS             4378..5958
                     /gene="cimA"
                     /locus_tag="PKX09_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOU17625.1"
                     /translation="MNRKIQIYDTTLRDGTQAETISFSADDKLHIAKRLDEFGIDYIE
                     GGWPGSNPKDMEFFKLARETEFRHARLVAFGSTKRAGNAIENDQNIQNLLEAGTLAVA
                     IFGKSWILHVEKALRITPAENLELIRESIAYLKSKGKEVIYDAEHFFDGYKDNADYAL
                     QTLQTAQSAGADLITLCDTNGGTLPSEISRIISEVRQKITAPLGIHTHNDSGLAVANS
                     LAAVEAGCVLVQGTINGYGERTGNADLCSIIPNLQLKAGYQCIPSENMKQLTPLSRYV
                     SEIANLAHPNDLPYTGRSAFAHKGGVHVSAVMKDPRSYEHIEPESVGNTRRVLVSDLS
                     GQSNIIFKAQELNIDLERHQDKIPEIVTKLKELEHLGYQYEAAEGSLELLISKIKSEY
                     HDFFKLKGFRILLEKNEDGEQRSEATIRMEVNNTIEHTAAEGDGPVNALDNALRKALT
                     KFYPEIAEMKLSDYKVRVLNPQQATAAKVRVLIESSSDGETWGTVGVSENIIEASWQA
                     LTESISYYLMKKRQKNSK"
     gene            5948..7516
                     /locus_tag="PKX09_07110"
     CDS             5948..7516
                     /locus_tag="PKX09_07110"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOU17626.1"
                     /translation="MQNRRRFNMAKRIIIFDTTLRDGEQSPGASLTASEKLIIARQLA
                     NLGVDVIEAGFPIASEGDFEAVKQIAQSIEGPIICGLARTNEIDVDRCWEAVKYAAKP
                     RIHTFIATSNIHLEKKLHKTGAEVIEMAYSAIRHAKQYCADVEFSPEDCARTSLEYMC
                     EIVQAALEAGATTINIPDTVGYAEPEEFAERIRYLIDKVPAIRQAVISVHCHNDLGNA
                     VANSLAAIRAGATQVEGCINGIGERAGNAALEEVVMNLVTRQDYFDAETGINTREIFK
                     TSRLVANLTGLSIPRNKPIVGQNAFSHEAGIHQHGVLSSRQTYEIMTPEDVGWEGENL
                     VIGKHSGKHAVAALLEAEGYRPDPEQLNQITNKVKDLADKQKEVAAEDILAIARDVTM
                     QLPEEEQHIQLEEFTVTTGSGLTPTASVRLVVNGERKIGAATGDGPIDAISNAIWSVI
                     GPSLQLVEYSLKAITGGTNALADVSIKLADQKGNIFAARAVNEDVIKASAMGIVKGIN
                     KALSSKQKIHPHTV"
     gene            7538..8800
                     /gene="leuC"
                     /locus_tag="PKX09_07115"
     CDS             7538..8800
                     /gene="leuC"
                     /locus_tag="PKX09_07115"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOU17627.1"
                     /translation="MGATITEKILATHSGREHVKPGELVDCKIDTVMCHDVTTTPAIE
                     LLEKYAIPKVFDPARIVIMPDHFVPNKDIKSAEMVARIRRWAQAQGITQFYDIGCHGV
                     CHALLPEQGFVTPGKTIVCGDSHTCTHGALGAFATGIGSTDLAATLATGKLWFKVPES
                     MRFVVSGRLADGVYAKDIILKIIGQIGVDGALYRALEFCGEVIDELSVEGRMTICNMA
                     IEAGGKSGIINPDAKTLEYVRARTSDEFTVYRSDSDAEYYRTIEIDVSDLEPLVALPH
                     LPSNVKPVRELPDIPVDQAYLGSCTNGRIEDLRIAAQILKNRTVARGTRMIVVPATTE
                     IWKQANREGLLDIFMSAGATVSTPTCGACLGGYMGILASGERCISSTNRNFVGRMGSP
                     KSEVYLASPATVAASAITGKITDPRSYL"
     gene            8810..9298
                     /locus_tag="PKX09_07120"
     CDS             8810..9298
                     /locus_tag="PKX09_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014684490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOU17628.1"
                     /translation="MQGNAHVYARDHINTDEIIPARYLNIDREAELAEHAMEDIDVNF
                     IKKVKPGDFIVSGDDFGCGSSREHAVWALRGAGIKAVIANSFARIFYRNCINNGFLAI
                     ECPGAVRKISDGDRLEIDLAGGKIVNLTKNQTYQFQPLPDFARKIIEAGGLMEQIKKE
                     LA"
     gene            9299..10402
                     /gene="leuB"
                     /locus_tag="PKX09_07125"
     CDS             9299..10402
                     /gene="leuB"
                     /locus_tag="PKX09_07125"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013745492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOU17629.1"
                     /translation="MEVKKIAVLAGDGIGPEIMNEALKVLNAVQQKSEIRFEFREALV
                     GGAAWEKYGQHLPDETLQICRDSDAILFGAVGGPVEEQFSPKWRGVEVNALLGLRKTF
                     DLYANLRPAKLFPALVFASPLRQDIAEKGFDILVVRELTGGIYFGQPKGRRGNGAEEV
                     AFDTMIYSRREIERIAHVAFKAARQRRRHVTSIDKANVLTTMVFWREVVTEVAQQYPD
                     VTWESMYVDNASMQLVKNPAKFDVMLCGNMFGDILSDEAAAIAGSLGMLPSASLAENL
                     FGLYEPGGGSAPDIAGQNIANPIAQILSAAMMLTYSFGMNEAAMAIEQAVSDVLQAGY
                     RTADIWQRGCHKIGTAEMGDRIVAELLKKINPE"
     gene            10467..12731
                     /locus_tag="PKX09_07130"
     CDS             10467..12731
                     /locus_tag="PKX09_07130"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOU17630.1"
                     /translation="MDVDFINNFYCEFAERVSRLRSELGRALSFAEKTIYGHLFSEQP
                     QSKLKPRESYVNLRPDRVAMQDATAQMALLQFINAGRRQVAVPTTIHCDHLIVAKDDA
                     VTDLKRARRENREVYDFLQTAAYKFGIGFWLPGEGIIHQIILENYAFPGGLMIGADSH
                     TPNAGGLGMLGIGVGGADAVDVMAGLPWELKMPGLIGVELTGRLNGWASPKDVILKLV
                     GMLGTKGATGMVIEYFGDGAANLSCTGKATICNMGAECGATASIFPYDAKMAEYLTLT
                     GRAAVAKAATAAFDSILQADPEVYQTPQKYFDRVIKIDMDNLEPYVNGPFSPDTAVPL
                     SQLRDHIKKSGYPAHLSAGLIGSCTNSSYEDLSRAADVARQANRHKIATRAKLLVNPG
                     SQWVKSVCEKAGFLNEFRTLKAQILANACGPCIGQWERDDCKPGIKNSIITSFNRNFA
                     GRNDGNQQTHTFLASPEIVLALTLAGDLNFNPLTDFLISEDGKRYQLEPPEGEELPAV
                     KIQTSRAPVRIVHKPDITIKIDPLSQRLRLLEPFPAPTSQSFQNLLLLMKVKGKCTTD
                     HISPAGEWLKYRGNLDRISDNLLLGARNAFTGKLGETLNQLTGEYAKVADVARSYQSH
                     QKLSVIVADENYGEGSSREHAAMEPRYLGVVAVIARSFARIHETNLKKQGVLALTFTQ
                     FDDYERIREDDEFSIQSLEKFAPERPLDLIIQHKNGESETIQLQHSYSQLQIEWFWAG
                     SALNYLKLNNPQKL"
     gene            12749..13999
                     /gene="icd"
                     /locus_tag="PKX09_07135"
     CDS             12749..13999
                     /gene="icd"
                     /locus_tag="PKX09_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009791360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOU17631.1"
                     /translation="MSNEQIVFANGSLQVPNFPLIPFIEGDGIGAEICRPTLRLIDAA
                     VNRAYGAERRIIWREVLAGDKSLNQNGEALPAATLQDLNQYVIGLKGPLRTPVAEGRR
                     SLNVILRQTLDLYACVRPLHWFPGVPTPVRRPELLNVVIFRENTEDLYTGIEFMRGSL
                     EAGLLEDFLIGKLKAPEPRFPGENSLGVKPVSKPGSERLIRAAINFAIKYKLPSVTLV
                     HKGNIMKYTEGGFRNWGYELAEREFAESCITAKIGAPSGSLTSGSGDKVLIKDMITDA
                     FFQDILLNPARYSVIATLNLNGDYISDLAAAMVGGIGLAPGANINFETGRAIFESTHG
                     TAPDIAGHDIANPSSLIMSGVMMLEYMGWSEAANLLQKAMANLFIRQIGTVDIFGNVN
                     GARCVGTTEFVDLLEDEILKINIK"
     gene            14019..15371
                     /locus_tag="PKX09_07140"
     CDS             14019..15371
                     /locus_tag="PKX09_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOU17632.1"
                     /translation="MSNSMLQSISQKMQKASQIDPELYVKYNVKRGLRNEDHTGVLVG
                     LTRIGDVVGYRTENGQVIPIEGQLLYRGYQLDDLVKGFQAENRHGFEEICYLLLAGEL
                     PNRSELQYFSDYLAKQRELPENFTNQMLINLVSGDMMNMLARAVLALYTNDPQPEDRS
                     VENLISQSLSLIAKFPTIVAHSYNAYRHAYQQKTLSIRHPHKDLSIAENFLYMLKGNR
                     YTKLEADMLDLALVTHAEHGGGNNSTFTLRVTSSTGTDTYSAIAAAIGSLKGPYHGGA
                     NRKVMEMMTDIKKSLKNWDDRNAVADYLMKILKKEVGDRVGKIYGFGHAVYTLSDPRV
                     KILKSKAADLATEKNRLEEFHLYNLVEELAPQVFADFKGREKSKIVCANVDFYSGFVY
                     SCIGIPPELYTPIFAVSRLAGWCAHRLEELNPQSRRIIRPAYKNVTPEREYVNIAERR
                     "
     gene            complement(15575..17719)
                     /locus_tag="PKX09_07145"
     CDS             complement(15575..17719)
                     /locus_tag="PKX09_07145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU17633.1"
                     /translation="MRKKPLFLLSIFLFVTFIENVNAQWERIEGLYGGMILGVTGDNP
                     TMYAVSATRLLSKYYLYQSNDMGITWDNTYLDVTNYYDVAAKDSDIFVAFGYPTNNHT
                     CLIHSSDYGRTWESINGIFDSVYVQRISIKDSMVIVTNPYAGLFFSKDNGANWSRSDI
                     NNLPEGRQIWDIAYSDSAIFLSAENCLYRSTDNCVSWDQIYEGIHVIYCIASNGTNLI
                     LNTDYDFYISSDDGDSWTKLSSPDLGSTGGANKLVYYGSHLYFTNISQLFHSADSGNN
                     WTELKLGIDSSEIYRDIYIDSSGVYVATERGMIYSTIDGVNWSYMDLSGITSRSVEEF
                     EFCGNSIFTFSVPPTPYLGNIYSMSTNSWKPIITDPELSLSTFASFGNNQIIAGCRND
                     TSVLCYNENEDIWNKVGNVKTSMIRSTGTELYAIIDSIPYYNNIYKSTDGGYTWDRLL
                     IDDKFGKFTNIFIDDENIFVTKDIYDLPTLLLFRSKDSGNTWEFITADTFQINAVLTI
                     DSVMFMGTESSSYNLNGLLRSLDSGKTWDNIFPDKSISSALKRGHNLIFSSGNEVFRM
                     DYLNRSILNISDSLMGNINQLKINDDYLYAGTIRGLWRRPLSDFIVGTHRDRFSTITD
                     YALHRAYPNPFNPKTTFRFDLPEETHVEIVVYDLMGREVWKSAKTHYSAGTYSVVWNG
                     VNQSGQPVGTGMYIVRLKSLRYSATQKVLLMK"
     gene            complement(17951..19075)
                     /locus_tag="PKX09_07150"
     CDS             complement(17951..19075)
                     /locus_tag="PKX09_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17634.1"
                     /translation="MTSDKKFKLLLNTMIVTVLSLLLFLSSCPEEPDYYDDSDTTSHN
                     FIWQIDTLGLYGSYLNDVWIVDENNIWVVGEINISDPDSSWNGTGRETFNAARWNGTE
                     WEYIHIRGSLMNDTGPLYSIWYFDECNIWVTTFGLPIHWDGNKWTLYHIQNMGIDASA
                     GYGIWASSPSNIYFVGYKGSIVHNDGKNFRKMESGTDTQITDIWGTGAHHIWATAKSN
                     QLDDTHPNGYESVTLFCNGQQWTRQYESPIGHTTEKSATELKGYMNSVWAYKDTVYIS
                     SNSGLWKESIKKGKGQLMHGPDNRLNGYPFLVRGTGYNDVYAFTHWGEFLHYNGKTWN
                     RDLSLDGINVRNASVTEDMVVTAGDMQFRYIIIVRGHHIK"
     gene            complement(19291..20616)
                     /locus_tag="PKX09_07155"
     CDS             complement(19291..20616)
                     /locus_tag="PKX09_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17635.1"
                     /translation="MTSNNDLKSKNAMSIILLLTAIFFAMSCRECPTESENNIDLIVK
                     DIFCTSVVLKISLPYSGAVNTFALDRNDSTVATYTCSEDDTLIIDEGLTPATKYLYSV
                     RFLKDGKTKSESDPVAVHTMNITSHDISYWDIDTLGISGGIYDLWIVSEDNIWAVGQI
                     IAEDPDSSFDGSGEEWFNAAHWDGEKWNLIRILNTATLYSIWYFSESDIWVSSGFPKH
                     WDGQQWTMYHFQDMGLGIEVSTEHIWASSPNNVYFVGWKGSIVHYNGSNFTKMESGTT
                     CKLEDVWGIDGNHIWAVGTESDNSRSVILFYNGKTWNTLHDTNNERKCCSNSVWTDNP
                     YLLFLNGGSGRYLFDLMQEKLSKYESAGRWFGYDIYGTAYNDIFASTAGSEMLHYNGS
                     TWYLYPEVKAQFEDYFRIRAVKANDEIVVASGYYYSGIGGLPIIIRGYR"
BASE COUNT         5838 a         4581 c         4885 g         5698 t
ORIGIN      
        1 cccggctggg gaagttgttc gggacttttc actgctaact ctatgaattg tttgtgtgaa
       61 gcgcttggaa tagccctgcc cggcaacggt actattctgg ctgaagatga gcgccggatt
      121 gagctttata aagcggctgg ggcaagaatt gtagaactgg tgcgttccaa cttgtgccca
      181 tcacaaataa tcacgcgcgc ttctattgat aatgcgatcg tgatggatat ggcaatgggc
      241 ggctcgtcga atacagtttt acatttgatg gcggttgcga atgaagccgg cattgactat
      301 tcgattaatc gtatggatga aatttcacgg aagacgccga cgatatgtaa agttgcgcct
      361 tcttcacctt atcatattga agatgtagat gcggcgggtg gtatgagcgc tctgttgaac
      421 gaaattttcc gggaaaacga atttgtcaat aaggattgtt tgacggtgac cggaaaaacc
      481 attgcggaaa acgtcgccgg taagcgtatt ttagacgact ccgttatccg ccatctggaa
      541 aatccctatt ccccaactgg tggtttggcg ataatgaccg gcaatttagc gccggaaggg
      601 agcgtcgtta aatatgccgg agtttccgac aaaatgaagg tattcgaagg cccggcagtt
      661 atttttgaaa gccaggaaga ggcttgcgaa ggaattctat ccggcaaagt caaagccggt
      721 gatgtggtcg tcattcgcta tgaaggacca cgcggtggcc caggaatgca ggagatgctt
      781 tcgcccactt cctatattat ggggcgtggt ctgggtgaaa gtgtggcatt gattaccgat
      841 ggaagatttt ccgggggaac acgcggcgct tgtatcggcc acgtcgcccc ggaagccaca
      901 gtcggtggac caattgcttt acttaaaaac ggtgatcgta ttcgcattga tattccccag
      961 aaatcgctgg aggttttact ttctgattca gaattgcaaa gtcgtcgcgc ccaatggcag
     1021 gcgcgtccgc cacgcataac gcatggagta atggctcgtt atgccgaaat ggtagcttct
     1081 gccagtcagg gagcggtctt gaaatataac tcacgcataa aaggagaaaa gttgtaatga
     1141 aaggcgccga aattttaatt gatgccttaa ttaatgaagg ggttgatact atatttggtt
     1201 atccgggcgg cgtagttatt ccgatttttg atgtactata ccgaaccgat aaaatcagat
     1261 tcattctaac ccgccatgaa caaggtgcga ctcacgccgc ggatggttat gctcgcgcca
     1321 ccggcaaagt cggtgtctgt ctggtaactt ccggtccggg cgccaccaac acaatcactg
     1381 gaattgccaa tgcccgaatg gatagcattc cgattgtagt catcagtggt caggtggata
     1441 ccgctaatat cggcactgac gcttttcaag aaaccgatat gatcggattg actcgttcca
     1501 tcactaagca taattattta gtaaaaagcg tagaagaatt gccgatagtt cttaaagaag
     1561 ctttctacat tgcgcgcacc gggcgacccg gtccggtggc aattgatatc ccggttaatg
     1621 tttccaatgc ccaactgaaa aattatcaat atccgtctga agttactctg ccggggtaca
     1681 aaccggtgac taatggcaat cccaagcaaa tcgaaaagtt agcgcaagcc attaaaactg
     1741 ctaaaaaacc actgctttac accggtggag ggatcatctc ggctaatgct gagccggaac
     1801 tgcttgccct actcgataaa actaatatac cgattgtgat tactttgatg gggttgggtt
     1861 caattccatc tgattatccg actttcctcg gaatgcctgg tatgcatggt acaatggccg
     1921 ctaacttggc tttaaacgaa tgcgatttgt taatcggaat tggagtccgc tttgatgatc
     1981 gcatcactgg caaacgcgaa cagtttgcca ggcaagccac cgtagctcat attgacattg
     2041 atccagctga aattggtaaa gttatcaagc cgcacattcc ggtcgtaggt gatgctaaga
     2101 aaattctcag tgaattggtg aaaaaagtta gtccgcgtcc gaaagacgag tggaatgaac
     2161 gcactcttgc ctggaaaaat cagtacccga tgacttacga tcaagatgcg gaggaagcta
     2221 ttttgccgca gtacgtaatt gaccgggtta gtgccttggc tgatccggat gcgattcttg
     2281 ttacggatgt cggccagcat cagatgtggg cggctcaata ttatcggcat cgtaaaccgc
     2341 gctcgtttat ctcatccggt ggattgggga cgatgggatt tggcttgcca gccgcaatgg
     2401 gtgcggcagt tggtcgtccc gggcacaccg tcattaccat ttcaggtgat ggcagtattc
     2461 agatgaatat tcaagaatta gctacctgtt ccatcaataa aatcccggtc aaagtaatta
     2521 ttttaaataa ttcctatctg ggtatggtgc gtcaatggca ggagctgttt tgggacgaac
     2581 attattcaca gacctgtttg aagcgcagtc ctgattgtcc gccacaatgt actggcccac
     2641 gcaaggattg cccatcagtt tatattccag attttgtcaa acttgccgaa gctaatcgga
     2701 tttatggcta tcgagctact aagcccgaag aggtggatga tgtattaaag cagggactga
     2761 gccagcctgg tccggttatt atggaattcg tcgtgaaaag catcgaaaat gtctatccaa
     2821 tgatcccatc tggcaagacg atttcagatg ttattccagg gaggcaatca tgatcgaaaa
     2881 acatactatc attgttaccg tagtcaatcg agttggtatt ttggcccgaa ttactggttt
     2941 attggctcag cgcggttata atatcgaaag cgctatcgcc gctccaactg aagaaaagga
     3001 tatttataaa atcattttag tcgtcaaaga aaccagtgag aatatcgagc aaattaccaa
     3061 gcaactcaat aagctgattg atacaatcaa ggtaactgat atttcccata aagataacta
     3121 tattgtgcgc gaatatataa ttttaaaagt cggtcttact cctcgcactc gggcggaagt
     3181 gctggatttg atgcgagttt ttggtgccaa gccgattgat ttagccactg atcatttgat
     3241 tatggaatta gccggacacg tccgcaaaat cgatcggttt atcgaattac ttaagccatt
     3301 tggaatcgag gaatatgtcc gcagtggtga atttgctatg acagaatata attaataaaa
     3361 aaggagttag ctatgtcagt caaattgttt tatgagaaag atgttaaaag tgattccctg
     3421 aagaaaaaaa ccattgccat aattggctat ggtagtcagg gtcacgctca agcccaaaac
     3481 ttgcgtgaca gcgggtacaa agttattatt gctgaaatgc caggttctcc aaattatgcc
     3541 ttagccgtta agcatggttt caaacccgtc agcgcggctg aagccagtca acaagctgac
     3601 gtcattcaaa ttctgacgcc ggacgaggtt cagaagcgcg tttatgagca ggaaatcaaa
     3661 caaaatctca ccaaaggcaa agccctggtt ttttctcacg gattcaatat tcactttaaa
     3721 caaattgtac cgccggccga tgtggacgtt tatatggttg ccccgaaagg ccccggtcat
     3781 ttggttcgcc gtcaatacga gattggtgct ggcgtaccat cgctatttgc ggtttatcag
     3841 gatgttactg gtcaagccag gaacctagct atggaacatg cttgtggtat cggtgccggc
     3901 agagccggaa taatcgaaac aacctttcgc gaagaaaccg aaactgatct tttcggggag
     3961 caggcggtct tgtgcggtgg cgtttcggaa ttgatcaagg ccggatttca gactctggtg
     4021 gatgccggtt accaacccga aatcgcttac ttcgaatgtt gtcatgaact taaattgatc
     4081 gttgatttaa tttatgaagg cgggttagcc ggtatgtatt attccgtcag tgatacggca
     4141 gaatacggtg gaatgacccg tggcccgtac atcattgatt gcgaaaccaa gaaacggatg
     4201 cagactttac tgcgtgccat ccagactggc tcatttgcca aggattggat tctcgaaaat
     4261 caagctgggc gtcccacgct caatgcctac cgtcaggaac atcaggaatt attactggaa
     4321 aaagtaggtc gggaactgcg cgagatgatg ccctggctta agaaaaaagg ataaaatatg
     4381 aatcgtaaaa tccagattta cgatacgact ctgcgcgacg gaacccaggc tgaaactatc
     4441 agtttctctg ctgacgacaa gttgcatatc gctaagcgac tggatgaatt tgggattgat
     4501 tatatcgaag gcggctggcc gggctcgaat cccaaagata tggaattttt caaactggct
     4561 cgcgagactg aatttcgcca cgcccgccta gtagcttttg gttcgaccaa acgggccggc
     4621 aatgccatcg aaaatgatca gaatattcag aatttgctcg aggctggaac gctggcagtg
     4681 gcgatttttg gtaaaagctg gattttgcat gtcgaaaaag ccctgcgtat aacaccggcg
     4741 gaaaatctcg aattgatccg cgaaagtata gcttatttaa aaagcaaggg caaagaggta
     4801 atctacgacg ctgagcattt tttcgacggt tataaagata atgccgatta tgccctgcaa
     4861 actttgcaaa ccgctcaatc ggctggcgcc gatttaatca ctttgtgtga tacgaacggt
     4921 ggtactttac cttcagaaat aagtcgcatc atctccgaag tgcgtcagaa gatcaccgcg
     4981 cctctgggca ttcacactca taatgatagt ggtctggcag tggcgaatag tttagcggct
     5041 gtcgaagccg gttgcgttct ggtccagggt acaatcaacg gctatgggga acgcaccggc
     5101 aacgccgacc tgtgttcgat catcccaaat ctccagttga aagccggtta tcagtgtatt
     5161 ccgtcggaaa atatgaaaca gctgactccg ctttcgcgat atgtcagtga aatcgctaat
     5221 ctggctcacc ctaatgattt gccttacact ggtagaagcg cttttgccca taaaggggga
     5281 gtccacgtca gtgcggtgat gaaagacccg cgcagttatg aacacatcga gcccgaaagc
     5341 gtcggcaata ctcgtcgcgt gctggtctcg gatttatccg gccagagcaa cattatcttc
     5401 aaggcgcaag aacttaatat tgatctcgaa cggcatcagg ataaaattcc cgaaattgtt
     5461 accaaattaa aggaacttga acacctcggt tatcaatacg aagccgcaga aggttcattg
     5521 gagcttttga tcagcaaaat taaatccgaa tatcacgatt tttttaagct gaaaggattc
     5581 cgcatccttc tcgagaaaaa tgaggatggc gaacaacgct cggaagccac gatccgtatg
     5641 gaggtgaata atactatcga acatactgcg gctgaaggcg atggtccggt caacgccctc
     5701 gataatgccc tccggaaagc cctgactaaa ttctatcccg aaattgccga aatgaaattg
     5761 tcggattaca aagtgcgcgt attgaatccc cagcaagcga cagccgccaa ggttcgcgtg
     5821 ctgattgaat cttcctccga tggtgaaacc tggggcaccg tcggtgtttc ggaaaatatc
     5881 attgaagcca gctggcaagc cctgacagaa agcatctctt attatttaat gaaaaagcgt
     5941 caaaagaatt caaaataggc ggaggtttaa tatggctaaa cgcattataa tttttgatac
     6001 gactctgcgg gatggcgaac agagtcccgg cgcatctttg acggcttctg agaaattgat
     6061 catcgcccgg cagttagcca atctcggcgt ggacgtcatc gaagccggat ttccaattgc
     6121 ctccgaaggc gacttcgaag ccgtaaaaca gatagcgcaa agtattgaag gtccgattat
     6181 ctgcggtctg gcacgcacta acgaaattga cgtagatcgt tgctgggaag ctgttaaata
     6241 tgccgccaaa ccgcgcatac atactttcat cgctacatct aatattcacc ttgagaaaaa
     6301 attgcataag accggggctg aggtaattga aatggcttat tcagctatcc gtcacgcgaa
     6361 acaatattgc gccgatgttg aattttcacc cgaagattgt gcccgaacta gcctcgaata
     6421 tatgtgtgaa attgtgcaag ccgccctgga agccggcgct acaactatta acattcctga
     6481 tacggtcggc tatgctgaac cggaggaatt tgccgaacgg attcgctacc tgattgataa
     6541 agtgccggca atccggcaag cggttattag tgtccattgt cataatgacc tgggcaatgc
     6601 tgtagctaat tcgctcgccg ctatccgggc tggtgctacc caagtggaag gctgtattaa
     6661 tggaatcggc gaacgcgccg gaaatgccgc acttgaagaa gttgttatga acctggttac
     6721 ccgccaagat tatttcgatg ccgagaccgg gataaatacc cgcgaaatct tcaagactag
     6781 tcgtttggta gccaatttga ccggcctgag cattccgcgt aataagccga tagtcggaca
     6841 aaatgctttt tcgcacgaag ccggtatcca tcaacatggc gttctgagca gtcggcagac
     6901 ttatgaaatt atgacgccgg aagatgtcgg ttgggaaggc gaaaacctgg tgattggcaa
     6961 acattccggt aaacatgccg tggcggcttt actggaagcg gaaggctatc gccctgatcc
     7021 ggaacagctc aatcaaatta ccaacaaagt caaagacttg gctgacaaac aaaaagaagt
     7081 cgccgccgag gatatcctgg ctattgcccg cgatgtaact atgcaattgc cggaagaaga
     7141 gcaacatatc caactggaag aatttaccgt cacgaccggt agtggcttga caccaaccgc
     7201 ctcggtgcgc ttggtggtta atggcgaaag gaaaatcggc gcggctaccg gcgatggtcc
     7261 gattgacgcc atcagtaatg ctatctggag cgttatcggg ccctctctac aattggtgga
     7321 atatagtctg aaagccatca ccggcggaac gaatgcttta gccgatgttt cgatcaaatt
     7381 ggctgaccag aaggggaata tctttgccgc ccgcgccgtt aacgaggacg tcattaaagc
     7441 cagcgctatg ggaattgtca aaggcattaa taaagcgctt agtagtaaac agaaaatcca
     7501 tccccacacg gtctgaatta ataatttgga gaataaattg ggcgcaacta taactgaaaa
     7561 aattttagca acgcatagcg gccgggaaca cgtcaagcct ggtgaattgg ttgattgcaa
     7621 aattgatacg gtaatgtgtc acgatgttac taccacaccg gcaattgaac tgctggaaaa
     7681 atacgctatt ccaaaagtct tcgatccggc gcgcatcgtt attatgcctg accacttcgt
     7741 acctaataaa gacatcaagt cagcggaaat ggtggctcgc attcgccgct gggcacaggc
     7801 tcagggcatt actcaatttt acgatattgg gtgccatggt gtctgtcatg cccttctgcc
     7861 ggagcagggt ttcgtgacgc ccgggaaaac aattgtatgc ggcgatagtc atacttgcac
     7921 gcatggcgca ctgggcgctt tcgctactgg tattggtagc accgacctgg cggcaacttt
     7981 agcgactggt aaactctggt tcaaagtgcc agaatcgatg cggtttgttg tttcaggacg
     8041 cctggcggat ggcgtttacg ccaaagacat tatcctgaaa attatcgggc aaatcggtgt
     8101 ggatggcgcg ctgtatcgcg ctctggaatt ctgtggtgaa gtaattgatg aactatcggt
     8161 cgaaggaaga atgacaattt gcaatatggc gattgaagcc ggcggcaaat ccggcattat
     8221 taatcccgat gctaaaacac tggagtatgt ccgggctcgc actagcgacg aatttactgt
     8281 ttatcgtagc gatagcgacg ccgagtacta tcgtacaata gaaattgatg tttccgattt
     8341 agaaccgctg gtggctttgc cgcatttgcc cagcaatgtc aagcccgtac gcgagttgcc
     8401 cgatattcca gttgatcagg cttatctcgg cagttgtacc aatggtcgaa tcgaggattt
     8461 gcggatcgcc gcccaaattt tgaaaaatcg taccgtcgcg cggggaacgc ggatgatcgt
     8521 cgtaccggct accactgaaa tctggaaaca ggcaaaccgc gaaggattac tggatatttt
     8581 tatgtcggca ggagctaccg tcagcacgcc gacttgtggc gcctgcctcg gcggatatat
     8641 gggaattctg gcgtctggcg aacgttgtat cagctcgacc aaccgcaatt ttgttggacg
     8701 gatgggcagt ccgaaatcgg aagtttattt agccagtccg gcgacggtag cggcttcggc
     8761 gatcactggc aaaatcaccg acccgcggtc atatttgtag gagttgatta tgcaaggaaa
     8821 tgctcacgtt tatgccagag atcacatcaa taccgatgaa attattccgg cgcgatacct
     8881 gaatattgac cgagaagccg aattagctga gcacgctatg gaagatattg atgtcaattt
     8941 cataaaaaaa gtcaagcccg gcgactttat tgtcagtggc gacgattttg gttgtggcag
     9001 ttcccgcgaa catgcggttt gggcgttgcg tggggctggg attaaagctg taatagccaa
     9061 ttcgtttgct cgtatttttt accgtaactg tatcaataat ggtttcttag cgattgaatg
     9121 cccgggcgcg gtgcgtaaaa tttcggatgg cgatcgcctc gaaattgatt tagccggtgg
     9181 caagattgtc aatctcacga agaaccagac ttatcaattt caacccctcc ccgattttgc
     9241 ccggaaaatt atcgaagcgg gagggcttat ggaacagatt aaaaaggaac ttgcttagat
     9301 ggaagtaaag aaaattgctg tgttagccgg cgatggcatc ggtccggaaa taatgaatga
     9361 agctctgaaa gtgctgaacg ctgttcaaca gaaaagcgaa attcgattcg agttccgcga
     9421 agcgctcgtg ggaggagcgg cttgggagaa atacggtcag catctaccgg atgaaacact
     9481 ccagatttgc cgtgatagcg atgcgattct tttcggtgcc gtcggtgggc cggtagagga
     9541 acagtttagc ccgaaatggc ggggcgtgga agtgaacgcc ctcctcggtt tgcgcaaaac
     9601 cttcgattta tatgccaatc tgcgtccggc taaacttttc cccgccttag tctttgcctc
     9661 acctctccgc caagacattg ctgaaaaggg atttgatata ttggtagtgc gcgaactgac
     9721 cggcggaatt tattttggcc agccgaaagg ccgacgcggc aatggtgctg aggaagtcgc
     9781 tttcgatact atgatctatt cccgccgcga gattgagcgg atcgcgcatg tggctttcaa
     9841 agccgctcgt caacgccgcc gacacgttac cagcattgat aaagccaatg tattgacgac
     9901 gatggttttc tggcgggaag tggtcacaga agtcgctcaa cagtatccgg acgttacctg
     9961 ggaatcaatg tatgttgaca atgcttctat gcaattggta aagaatcccg ctaagttcga
    10021 tgttatgctt tgtggaaata tgttcggcga cattctttcc gatgaagccg ccgcaattgc
    10081 cgggagtctg ggaatgttgc ccagcgccag cttggcggaa aacctttttg gattatacga
    10141 accgggtggc ggttcggcgc cggacattgc cggacaaaat attgctaatc caatcgccca
    10201 gattttgagt gcggcaatga tgctaaccta ttcttttgga atgaatgaag ccgcaatggc
    10261 tatcgaacaa gcggtttctg atgttttgca agccggttat cgcacggctg atatctggca
    10321 gagaggatgt cataaaattg gcacagctga aatgggcgac cgtattgttg ccgagttatt
    10381 aaagaaaatt aatcctgaat aaaatattta taattataga gaaacgctca ttaaggaatg
    10441 ttatcagaat gaggagtaaa ttttaattgg atgttgattt tataaacaat ttttactgcg
    10501 aatttgctga acgagttagc cgattaagaa gcgaacttgg ccgagcccta agctttgcgg
    10561 aaaaaactat atacggacac ttgttttccg aacaacctca atcgaaattg aaaccccgtg
    10621 aatcgtatgt gaacctgagg cctgatcgag tcgctatgca ggatgcgacc gctcaaatgg
    10681 cattattgca gttcatcaat gctggtcgcc gacaagtagc cgtcccgact actattcact
    10741 gtgaccatct aattgtggca aaagacgacg cagttactga tttaaaaagg gcgcgacgag
    10801 aaaaccggga agtctatgat tttttgcaaa cagccgccta caaatttggt atcggtttct
    10861 ggctgcctgg tgaaggcata attcaccaaa ttatactgga aaattatgcc tttccgggtg
    10921 gtttgatgat cggagccgat tcgcatacgc ctaatgccgg tggcttagga atgctcggca
    10981 ttggcgtcgg tggggctgac gccgtagatg tgatggctgg cttgccgtgg gaattgaaaa
    11041 tgcccggctt gatcggagtc gagttaaccg ggcggctgaa tggctgggct tcacccaagg
    11101 atgtgatctt gaaattagtc ggcatgctgg gcactaaagg tgctaccggt atggttatcg
    11161 aatattttgg cgatggcgcg gctaatcttt cctgtaccgg caaagccaca atttgcaata
    11221 tgggagcaga atgcggcgcg accgcctcaa tatttcctta tgatgctaaa atggcagaat
    11281 atttgactct gaccggaagg gcggcggtcg caaaagccgc tacggcggct ttcgactcaa
    11341 ttctccaggc tgatccggaa gtctatcaaa ctccgcaaaa gtacttcgat cgagtgatca
    11401 aaatagacat ggataacctg gaaccgtatg ttaacggacc tttttcacct gatacggcag
    11461 taccgctttc acagttaaga gatcacatta agaaaagcgg ctacccagct catttatcag
    11521 ctggcttaat aggttcttgc accaattctt cctatgaaga tttgtcccga gcggcagatg
    11581 tcgcccgtca agcaaatcgt cataaaatcg ctacccgagc aaaattatta gtcaatccgg
    11641 gttcccaatg ggtcaagtcg gtttgtgaaa aagccggttt tttgaatgaa tttagaacgt
    11701 taaaagctca aattctggca aatgcctgtg ggccttgtat tggtcagtgg gaacgggatg
    11761 attgcaagcc gggaataaaa aactctatta tcaccagttt taatcgcaat tttgccggac
    11821 gaaatgatgg caaccagcaa actcacacct ttctggcttc gccggaaatc gtgctggctt
    11881 taacattggc tggcgattta aactttaatc ctttgactga ctttctaatc agcgaagacg
    11941 gcaagcgtta ccagctcgaa ccacctgaag gtgaggaact gccagccgta aaaatccaga
    12001 ccagccgcgc cccggtgaga attgtccata aaccggatat cactatcaaa attgatccgc
    12061 tctcccaaag gctacgttta ctggagccat ttcccgcgcc aactagccag tctttccaga
    12121 atttgttgct tttaatgaaa gtcaagggta aatgcactac tgatcacatt tctccggctg
    12181 gcgagtggct gaagtaccgc ggcaatctgg acagaatttc cgataacctt ctgctgggtg
    12241 cccgtaatgc ctttaccgga aaattgggcg aaacccttaa ccagttgacc ggcgaatatg
    12301 ccaaggtggc tgatgtcgcc agaagctacc aatcccacca gaaattatcg gtaatagttg
    12361 ccgatgaaaa ttatggtgaa ggctcatcgc gcgaacatgc cgcgatggag ccgcgttatt
    12421 tgggagtagt ggcggtaatt gcgcgctctt ttgcccgtat tcacgaaacc aatttgaaga
    12481 agcagggagt cctggcttta acctttacgc aattcgatga ttatgaacgt attcgggaag
    12541 atgacgaatt tagtattcaa agccttgaaa aatttgctcc cgaacgtccg ctggatttga
    12601 ttatccaaca taagaatgga gaaagcgaaa caatccaatt acagcatagc tacagccagt
    12661 tacaaataga atggttttgg gctggcagtg ccttgaacta tcttaaatta aataatccac
    12721 agaagctctg attttaggga ggcttaatat gtccaatgaa caaatagttt tcgccaatgg
    12781 ttcgcttcag gtgccaaatt ttccgctgat tccttttatt gaaggcgacg ggattggagc
    12841 tgaaatttgc cgacccactc tacggttgat tgatgcggct gtcaatcggg cttatggcgc
    12901 tgagcgacgc atcatctggc gcgaagtttt agcaggcgat aaatctttga atcaaaatgg
    12961 tgaagcatta ccggccgcaa cccttcagga tttgaatcaa tatgttatcg gcttgaaagg
    13021 tcctttacgg acgccagttg ctgaagggcg ccgctctttg aacgttattt tacggcaaac
    13081 tcttgatctt tatgcctgcg ttcgacctct gcattggttc ccgggtgtgc ctacgccagt
    13141 cagacgaccg gagctcttga acgtcgttat ttttcgagaa aacacggaag acctctatac
    13201 cggaatcgaa tttatgcgcg gctcgctaga agctggcctg ctggaagatt tcctgattgg
    13261 taaattgaaa gcgccggagc ctcgcttccc tggcgaaaat tctttaggag tcaaaccggt
    13321 ttctaaaccc ggttcggagc ggcttattcg agcggcgatt aactttgcca ttaaatataa
    13381 attgccgtcg gttacgctag ttcataaagg caatatcatg aaatataccg aaggtggctt
    13441 tcgcaattgg ggatatgagc tggctgaacg tgaatttgcg gaatcttgca tcacggctaa
    13501 gattggcgct ccatctggat cattaacctc aggatccggc gacaaagttc tgattaagga
    13561 tatgatcacg gacgcttttt tccaagatat attgctcaac ccggctcgtt attcggtaat
    13621 agctactctg aatttgaacg gtgattatat ctccgatttg gcggcggcaa tggtcggtgg
    13681 aatcggtctg gcgccgggtg cgaatatcaa ttttgaaacc ggccgcgcca tttttgaatc
    13741 aactcacggc acagcccctg atattgccgg tcacgacatc gccaatccgt cctcactgat
    13801 tatgtcggga gtaatgatgc tggaatatat ggggtggtca gaagctgcca accttttgca
    13861 aaaggctatg gcaaacttat ttattcgtca gattggtact gttgatattt tcggtaatgt
    13921 caacggcgct cgttgtgtag gcactaccga attcgttgat ttattagaag atgaaatcct
    13981 gaaaataaat ataaagtagc gattaaaagg aacttaatat gtcaaattca atgttacaat
    14041 ccataagcca aaaaatgcaa aaagccagtc agatagaccc ggaattatat gtcaagtaca
    14101 atgtaaaaag aggcctgcgt aacgaagatc acacgggagt tctggtcgga ttaacccgca
    14161 tcggcgatgt agttggctat cgtacggaaa atggtcaggt aattccgatc gaaggccagt
    14221 tgctctatcg gggctatcaa ctggatgatt tggttaaagg ctttcaggcc gaaaatcggc
    14281 atggctttga agaaatctgc tatctgcttc tggcgggcga actgccgaat cgttccgaac
    14341 tgcaatattt ttccgattat ctagctaaac agcgcgaatt gccagaaaat ttcactaacc
    14401 aaatgctgat taatttggtt agtggtgata tgatgaatat gctcgctcgg gcggttctgg
    14461 cgttatatac caacgatccc caaccggaag atcgaagtgt agaaaatctt atcagtcaat
    14521 cgctaagtct aatcgccaaa tttccgacca tagtagccca ttcgtataat gcttaccgac
    14581 atgcctatca gcaaaaaacc ttatctattc gtcacccgca taaagattta agcatagccg
    14641 aaaattttct atatatgctt aaaggcaatc gctataccaa attggaagcg gatatgctcg
    14701 atttagcttt agttacccac gctgaacatg gcggcggaaa caactcgacc tttaccttac
    14761 gagtaaccag ttctaccggg accgatacct actcggcaat cgcggcggct attggttctc
    14821 tgaaaggacc ttatcacggc ggcgcgaatc gcaaagtaat ggaaatgatg acggatatca
    14881 agaaaagttt gaaaaactgg gatgacagga atgctgtggc tgattattta atgaaaatat
    14941 tgaaaaaaga agtcggggat cgcgtcggta aaatctatgg atttgggcat gccgtttata
    15001 ctctttctga tccgcgagtg aaaattttaa agagcaaagc tgcagatttg gcgacagaaa
    15061 aaaatcgtct tgaggaattt catctctata atttagtcga agaacttgcg cctcaggtct
    15121 tcgcggattt caagggacgc gaaaaatcta aaatcgtctg cgccaatgtt gacttttact
    15181 cgggatttgt ctattcttgt atcggaattc cgccggagct ttatactccg atttttgccg
    15241 tcagccgact ggctggttgg tgcgcccatc gtctggagga actcaatccg cagtcgcgcc
    15301 gtatcattcg accggcatat aaaaatgtta ctccagaacg tgagtacgtg aatatagcag
    15361 agagaagata aaatacatta ttcaatggtt actcaacatt cgttggtagt catttgtggt
    15421 tattgattgc gatacgccgt tgtttacagg atgaactttg gctgtatcag caaccaattt
    15481 tgcccggcgg ataaaatcgg tttagaagca ttcgatgggt aaaaataaaa ccctcgtagt
    15541 agaaaaccac ttcagggagg atttttaaac ggaactactt cataagtaat actttctgag
    15601 tagcagaata cctgagtgat ttcagtctga cgatatacat tccagtcccg accggctggc
    15661 cggattgatt gacgccattc cagacaactg aataggttcc ggcggagtaa tgtgttttgg
    15721 ctgatttcca gacttcgcga cccattagat cgtaaaccac gatctcgacg tgtgtctctt
    15781 caggaagatc gaaccgaaag gtcgttttcg gattaaacgg attgggatag gctcgatgaa
    15841 gagcataatc tgtgatagta gaaaatctgt ctctatgagt tcctacaata aaatctgata
    15901 gtggtcttcg ccaaagcccc ctgatagttc cagcatataa gtaatcatca ttaattttaa
    15961 gttgattaat attccccatt agactatcac taatatttag gatacttctg ttcagataat
    16021 ccattctaaa tacttcattg cctgatgaaa atattaaatt atgtcccctt tttaatgctg
    16081 acgatatact cttatctgga aaaatattat cccaggtttt accactatca agagatctta
    16141 ataaaccatt taggttgtaa gagctggatt cggtacccat aaacataact gaatcaatcg
    16201 tcaacacagc attaatttgg aaagtatctg cggttataaa ttcccatgta tttccagaat
    16261 cttttgatct gaataataac agggtaggca aatcgtatat atctttggtt acaaatatat
    16321 tttcatcatc aataaaaata tttgtaaact tcccgaattt atcatctatt aaaagtcgat
    16381 cccatgtata ccccccatcc gtcgatttat atatattatt gtaataaggg atgctatcaa
    16441 tgatggcata caattctgtt cctgttgacc taatcattga cgttttaaca ttacctactt
    16501 tattccatat atcttcattt tcattataac aaagtacact ggtatcgttt cgacatccgg
    16561 cgattatttg gttatttcca aaacttgcga aagttgagag ggataactct ggatcagtga
    16621 tgattggttt ccatgaattt gtactcattg agtaaatatt tccaagataa ggagtgggtg
    16681 gtaccgaaaa agtaaatata ctatttccac aaaattcaaa ctcttctacc gatcttgagg
    16741 tgatgccact caaatccatg taagaccaat tgacaccatc aatagtggag tatatcatac
    16801 ctctttccgt tgctacatat acacctgaac tgtcaatgta aatatcccta tatatctcag
    16861 aggaatctat tcctagttta agctctgtcc aattatttcc actatcagcc gagtggaaca
    16921 attgactgat atttgtaaaa tataaatgcg agccgtagta aactaattta ttggcgccac
    16981 cagtcgatcc aagatcagga gaagataact tggtccacga atctccatca tcagaggaga
    17041 tataaaaatc ataatcagtg tttaagatta aattagtccc attcgaggcg atacaatata
    17101 tcacatgaat tccttcataa atttgatccc aagacacaca attatctgta gatcggtaca
    17161 ggcaattctc agcagataag aatattgctg aatctgaata agcaatatcc catatctgtc
    17221 taccctcagg cagattattg atatcagacc ttgaccaatt tgcaccatta tcttttgaga
    17281 aaaataaacc ggcgtaagga ttagtgacta ttaccatgga atccttaata cttatacgtt
    17341 ggacgtagac agaatcgaaa atgccattga tactttccca ggttctacca taatccgatg
    17401 aatgtattaa acaagtatgg ttatttgttg ggtagccaaa tgccacaaag atatcagaat
    17461 ctttagcggc aacatcataa taattggtaa cgtccaagta tgtattatcc caggtaattc
    17521 ccatgtcatt cgattgatat aaatagtact tggataacag gcgtgtagct gagactgcat
    17581 acatcgtagg attatcgcct gtaactccaa gtatcattcc accgtacaat ccttctattc
    17641 tttcccattg tgcatttaca ttttcaataa aagtaacaaa taagaatata ctcaataaaa
    17701 ataacggttt ttttctcatg attggctcct ttcaaaaaat gttaatgact tgctacagtt
    17761 taatgccata tagtcttctt tgccagactt ccatttaaat tgagaataaa aaaatgcttg
    17821 gcattttgtt gtgtgataac cataccgttt gacttcgagg catagcatta atttttccca
    17881 ttctacatgg attatttagc caatattgat aaagtcaaaa taatttgagg tatttcatgt
    17941 ataaatcctt tcattttata tgatgccctc tgacaattat aatatacctg aattgcatat
    18001 ctccagccgt taccaccata tcttccgtaa cactggcgtt cctgacgttg atcccgtcca
    18061 ggcttaaatc ccggttccag gtcttgccat tgtaatgcag gaattcgccc cagtgggtga
    18121 aggcatagac atcgttataa ccagtgcctc gtaccaaaaa cggatagccg ttcaaacgat
    18181 tatcgggtcc atgcatcagt tgacctttgc ctttcttaat ggattccttc cagagtccgg
    18241 aattggatga aatatagacc gtatccttat atgcccatac cgaattcatg tagcctttaa
    18301 gttcagtggc gcttttctct gtagtatgac caataggtga ttcgtattgt cttgtccatt
    18361 gctggccgtt acagaataga gtaacgcttt catacccatt gggatgtgta tcatccaatt
    18421 gatttgattt cgcggtagcc cagatatgat gggcaccagt gccccaaatg tcagtgattt
    18481 gagtatctgt tccactttcc atcttcctga aattcttgcc atcgttgtgg acaatcgaac
    18541 ccttataccc tacgaaatag atgttagagg gagaagatgc ccagatacca taaccggcgc
    18601 tggcatcaat acccatattt tgaatatgat ataaagtcca tttgtttcca tcccaatgaa
    18661 taggtaatcc gaaagtagtg acccaaatat tgcattcatc gaaataccag atactatata
    18721 gtggaccggt atcattcata agactacctc ttatgtggat atactcccat tcggttccat
    18781 tccaccgagc ggcgttgaag gtctcgcgtc cggtgccatt ccaggatgaa tccggatcgg
    18841 atatattgat ttctcccacg acccagatgt tattctcatc tacgatccaa acgtcattta
    18901 gataactgcc atagagcccg agcgtgtcaa tttgccagat gaagttatgg ctggtggtat
    18961 ccgaatcatc atagtaatcc ggttcttcgg gacagctgga aaggaataga agtagagata
    19021 gaacagtcac aatcatagta ttcagaagta gtttaaattt cttgtcagag gtcatcgttt
    19081 ttccttattt tgttctttcc acaattgaaa atacactcat ttcagcgtaa tttcaaccac
    19141 gtgtttccct gatggaatcg ggatactgat cctgaatccg accgtgtccg cggtacggat
    19201 tcaggtgttg gcgggaattt cagttgcata tcaccctcta gtggttgggg attctttttc
    19261 attttttaaa tcctcttaat taataggttt tcaacgatac cctctgataa tgattggaag
    19321 acctccaatg ccggagtaat aatatccact agcgactact atttcatcgt tggctttaac
    19381 cgcccttatc cgaaaataat cttcaaattg tgcttttact tcaggataaa gataccatgt
    19441 cgagccatta taatgcaaca tttcactgcc agcagtagag gcaaaaatat cattataagc
    19501 agttccataa atatcataac cgaaccacct tccagcgctt tcatatttac tcaatttttc
    19561 ttgcatcaga tcaaataaat atctgccact gccaccattg agaaaaagta agtacgggtt
    19621 atcagtccac accgaatttg aacagcattt cctttcatta ttggtatcat gtaaagtatt
    19681 ccacgtcttg ccattataaa aaaggataac acttcggctg ttatcagatt ctgttcccac
    19741 cgcccagatg tggttaccat ctattcccca gacatcttcc agcttgcaag tcgttccgct
    19801 ttccatctta gtaaagttcg aaccattata atggacaata gagcctttcc agccaacaaa
    19861 gtagacatta ttaggagatg aggcccatat atgttcagtg cttacttcta tacccaatcc
    19921 catatcttgg aaatgataca ttgtccattg ttgtccatcc caatgtttgg ggaaaccgga
    19981 gcttacccag atatcacttt cagaaaagta ccatatacta tatagagtcg ccgtatttaa
    20041 tatcctaatc agattccatt tttcaccgtc ccagtgcgcc gcgttgaacc attcttctcc
    20101 ggagccgtca aaggaagaat ccggatcttc agcaatgatc tgaccgaccg cccagatatt
    20161 atcttccgag acaatccaca gatcatatat gccgcccgaa atccctaggg tatcgatgtc
    20221 ccaatacgaa atgtcgtggc tggtgatatt catagtatgc accgcgaccg gatcggattc
    20281 agacttggtc ttgccatctt tcaaaaaacg gacgctatat aaatattttg tagccggagt
    20341 taagccttca tcaataatca gggtatcatc ctcagaacag gtataagtcg ctacagtcga
    20401 gtcattccgg tcgagcgcaa aggtgttaac agccccggag taaggtaaac ttattttcag
    20461 aactactgag gtgcagaaga tatctttaac aatcaaatca atgttatttt cagattcagt
    20521 cggacattcg cggcaggaca tagcaaaaaa gatggcggtt agcagtaaga tgattgacat
    20581 tgcattttta gattttaggt cgttgttaga ggtcatcgtt tttccctttt tgttcttttc
    20641 acaattgaaa atacactcat tttagcgtaa tttcaaccat tgtgtgtttc cgccactggc
    20701 gaatagctcc cgcgcccgga ctgctgatac agtcagacgc gggtatgtac aggaattttg
    20761 agttgcatat tcccctccgt tagttgtatg ttcttgcgtc tcaacatggt agcgttgaga
    20821 cgaagggaaa aattataact ataactaata aattaggaca atcctgtcaa tctcgttatc
    20881 cgtgtcagaa aaattttaca ggtagcgctt gagggcgcgt tgcaattcgg cgaagtggag
    20941 cgtcaacgct atgcggtgcg aggaaccgaa ttcggcgtac gagccgccca gccccagccc
    21001 at
//
LOCUS       DAOSMP010000037        20258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_2642_length_20258_cov_17.475028, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000037 DAOSMP010000000
VERSION     DAOSMP010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 20258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20258
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2642_length_20258_cov_17.475028"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="PKX09_07160"
     CDS             complement(<1..1110)
                     /locus_tag="PKX09_07160"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOU17636.1"
                     /translation="MNAESRFLRAVCRVALDETENQIAVDNVVFNEDKLYNLGLYHQI
                     LPLIADYRVTLSKIFPAISATFFEKCRINALESAGRLIVYEKFLANLDQLFWAASIEY
                     RLFKGPVLAYELYEFPYQRPFGDLDILIQPESLEKVHTILGELGCELDDDLYTNFPAE
                     IIKKYRFARHYISVKNPLVAIDVHTGLSSRLHPFHFDFADFWSHSRIIKIGDREYQTF
                     DLSHQALYLLYHAFKHYYFKLIWIIDCRQNTKLPDFDKADFQHLIKTYNLTRVWNIFS
                     RVSSDLWNDSPQLFEYPKVRCSGLSYKINTEVILQGGLFTSLARARILLPLIYLPRLS
                     QKIAYLWRQMFPPRAVMWDFYSSPNLKPTWRNYLRL"
     gene            complement(1235..2044)
                     /locus_tag="PKX09_07165"
     CDS             complement(1235..2044)
                     /locus_tag="PKX09_07165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOU17637.1"
                     /translation="MSGRTKSEDFQLILAPDHIYNIDCVIGMQKMPQDFVDLIITDPP
                     FAINFKAQRNNYHRTPSRVLEGYTEIPADEYLNFTLKWMSQAYRILKPSGSMYVFSGW
                     NNLKEVLIALDQVGFVTVNHLIWQYQFGVVTRRRYVTSHYHCLYVCKNDRQRKFFPYS
                     RFDKDARDLSGSSLHYRDKEDVWYIKREYWTGKEKTPTKLPAEVIKKILQYSSSEDDL
                     VCDPFLGSGQVAVVSKMLKRHYIGFEINPVYYEFALKRLMPDNDLFNARAV"
     gene            complement(2106..4505)
                     /locus_tag="PKX09_07170"
     CDS             complement(2106..4505)
                     /locus_tag="PKX09_07170"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOU17638.1"
                     /translation="METMNGNIKTGIPERSFTETEKEVSLQDYFRIIYRGRWIIGIVF
                     VVVMLLTIYITFTTPPEYQASTTLMLSGRAASTSTLFQNPLLPMSYMKVNNEIEVLKS
                     TALARNVIKALRNSPYADSLYLLETRKYHKKGVGLGDLMGGITSGLQHIFMPSQEEDL
                     MVVDTLDRAIVKNLMTSMKVEPIRETEAIRLTISSVDPIEAALIANTIASEYYKTDVE
                     YNLGEVIEMKNFLEEQLEKIEKDLKESEEALKNYQRQEGIFGLDETAKILIDQLSTFE
                     ATYYTTLADLKIAQQTLDNQQRMLTEREQMVIQESVNTTNPFVAQLRQSIAEMEAEKI
                     TVMTVQGYSADHPAIKELDKRITDLKQKLFEEAEKITNLGLTPEEQSKISAELLNKVI
                     MNNIEVISLEAKCNEYKKLVDQYSARLDRLPASTLEYARLDRERQVNEKYYILMKTKL
                     QESRITEASKISNVRIIDPAIPPEKPVKPKKKMNLLLGLFLGLGAGVGIAFIRDYLDH
                     SIKSGEDLQRLGLTAIAIIPTIDVKSAVEKIGKVYKDKNNGEEVLVKARLVSHYDPKS
                     AVAEAYRTLRTNIQFMSPDRPIKSLLVTSAGPGDGKSTTVINIAITFANLGKKVLLID
                     ADLRKPIQHKVFEFQRAPGLTHLLIENLPDEEVIHQTEVPNLDLITCGDIPPNPSELL
                     ASQRLKNFMERAREKYDIILFDTPPVIAVTDATVLSRLTDAILLVVRAGSTDLRVLER
                     SIELLRQVKANLVGAVLNGVNMSTGYDSYYYYYRYYSYYSDTGAKKKRASRTRRHHHQ
                     A"
     gene            complement(4515..5537)
                     /locus_tag="PKX09_07175"
     CDS             complement(4515..5537)
                     /locus_tag="PKX09_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOU17639.1"
                     /translation="MKNNSSNIWKKLISPKTLFAFAISILGLYLGFRKFDSTEFLSYL
                     RLTNLWIFGLSMAVMVFLIGIRGWRWKYLILPINDLPVKQVFEMEMIGYFGNHVFPFR
                     MGEILRGYALSKTQNIPVSAVFGTIVLERTLDVLTFIIVLISGAIFFPGMPKWVRTSG
                     LISGIVILILFILVYISNLKKVALKRFFKQKMLKIKNLDDSKIGKTVKNFWQGLTTLR
                     ETPHLGLIIFQSFLIWAVNIGNFWLMGASLQVYFDLFDLLLVFFVTSAIISVPSAPGY
                     VGTYHAATIGILVFLGLELSKAQAVAVIMHAVGFISLTTLGLFYFLKYQLNFKSTVEV
                     YVEKGA"
     gene            complement(5534..6184)
                     /locus_tag="PKX09_07180"
     CDS             complement(5534..6184)
                     /locus_tag="PKX09_07180"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOU17640.1"
                     /translation="MKLVTRKYIFNSIILSVLLTIPILLLAQETTQVILKKVAHCYQS
                     AVPFKIGFTVRLAMPDNANLSKYSGIFFLGSDNQFRVSQTEEEIVYDGQWLWTYDKVN
                     RQVIVEEFNPRSSLKLIWDILSGSFDGYQISKSECQNGLLAVVELKPSSKDGCIRSLQ
                     MKIDRERGRIVSAEYRDFQDNLVNIELDTLIALGRADTGLFKINLNEGDELIDLRP"
     gene            complement(6238..8454)
                     /locus_tag="PKX09_07185"
     CDS             complement(6238..8454)
                     /locus_tag="PKX09_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOU17641.1"
                     /translation="MNNRQQEILGILLIALGILIMLSLISYNPIEEPTISKNVAIQNW
                     MGIVGVYISHYLIKYTLGVAVFIFPVLMLVWGWWLFADKERKILIRFSLYSIFMALLV
                     SVALGLPAVRENVDSNFGFRYSGLVGGVFAKVMCDWLGFTGTVIVLLVLALVTIRGYF
                     TWSFYSPIAKWRSHRHPKKERTSGEIPQPRIPEREFSVSQSEKDAAVVQSETEATRPV
                     KIKKSVEPQPPIIEPKVEPVTKSETSGGVYQLPPIDLLELPPESRDSVTKEEMAENAQ
                     LLENALETFGVKGKVVNVSPGPIITRYEIEPAVGVRVSKIAALADDLARILSAKRIRI
                     VAPIPGKSVVGVEIPNRNPEIVYLRSIIGSRQFASAKSKLTIALGKTTSGEIYTLDLA
                     RMPHLLIAGATGSGKSVCINTIIASLLYLARPDEVKFMLIDPKKLELSIFKALEGYHL
                     ITCEDLDEDVITRADNAIVALRSVELEMERRYEILASATVRNIDEYNNRVVSGELDKE
                     FLPYIIIVIDELADLMLTSAKEVEEPITRLAQMARAVGIHLIIATQRPSVDVITGVIK
                     ANFPARIAFQVASKIDSRTILDLNGAEKLLGRGDLLITTPQNPEPFRVHSAYISLEEI
                     ERILEHIENQPRPGQVELPSVKEESVEEFGLYDDSEKDPLYYEALRLVVTHQQGSISL
                     LQRRLKIGYSRAARLIDELEKASIVGAFTGSKAREVLVDEDYLEQLNKIQNDEDEV"
     gene            complement(8451..9557)
                     /gene="gcvT"
                     /locus_tag="PKX09_07190"
     CDS             complement(8451..9557)
                     /gene="gcvT"
                     /locus_tag="PKX09_07190"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOU17642.1"
                     /translation="MESANNQVKKTPFYDQHLACNAKLVPFAGYWMPIQYRGIMEEHR
                     RVRTTVGVFDVSHMGEFIVRGSSAEDFLNYITINDVSKLAIGQVQYSAMCYPDGGIVD
                     DLLVYRFADHFMLVVNAANLEKDFNWIKQHLPAGVELTDISDETGLLAVQGPLALETL
                     QPLTPINLNEIEFYHFREGEIANVPAILSRTGYTGEKGLEIYHKPADSARLWEAIFQA
                     GAKYDIQPIGLGARDTLRLEMKYALYGNDIDQTTNPLEAGLGWITKLDKPDFIGKAAL
                     LKIKAEGLKRKSVGFEMLEPGIPRHGYKVFKNGQEIGLVTSGTQSPTLNKGIGIAYVA
                     SEYSAVGTPIEIEIRGKLLKAVIVKTPFIPARTQ"
     gene            complement(9567..10160)
                     /gene="ruvA"
                     /locus_tag="PKX09_07195"
     CDS             complement(9567..10160)
                     /gene="ruvA"
                     /locus_tag="PKX09_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOU17643.1"
                     /translation="MFAYISGKLFQKEPTSVIIDVNGIGYQINIPLSTYSALPATGQN
                     AKLLTYYHVREDIQALYGFATPEEKELFLLLISVSGIGPKMAMTILSGTSPEQFRRRI
                     LDGDVESLTLIPGIGLKTARRIIVELGEKIGQTSEVVSGELATVTLGATGEEALRALI
                     SLGYRRAEALNALRKAVKELGDSAPVEKYIKAALNQM"
     gene            complement(10164..10655)
                     /gene="ruvC"
                     /locus_tag="PKX09_07200"
     CDS             complement(10164..10655)
                     /gene="ruvC"
                     /locus_tag="PKX09_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOU17644.1"
                     /translation="MSEELRVIGVDHGINRTGYAIVSKNGSKLSCLTHGSIVTTPREA
                     FNLRLKHIYTELLQIIEAWKPSIMVVEEAIYAQNIKTALLMGHARGAVLLAGANAGLT
                     IAEYPPTKIKSAVVGNGLASKEQVQYMVSRILGLSQEPESFDAADAMAAAICHLNRPN
                     IRD"
     gene            complement(10655..11413)
                     /locus_tag="PKX09_07205"
     CDS             complement(10655..11413)
                     /locus_tag="PKX09_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU17645.1"
                     /translation="MSGHSKWSTIKRKKGALDAKRGKVFTKIIKEIQVAAKMGGGDEN
                     ANPRLRTAVLAAKAANMPLANVERAIKKGTGEIESVNYEEITYEAYGPGGVAILMEAL
                     TDNKNRTVSELRHILTRHGGNLATTGSVAYNFEKKGIITVNKDTCTEDDLLVIALEAG
                     ADDISTEDDVYEVSVDPGHFEKVKKALESAKIAIASAAVTMIPKTTIKVDEKNAPKII
                     SLMDALEDHDDIQNVYANFDIDDKLLAELQQSAG"
     gene            complement(11444..11926)
                     /locus_tag="PKX09_07210"
     CDS             complement(11444..11926)
                     /locus_tag="PKX09_07210"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOU17646.1"
                     /translation="MKNKAIVVLILLSFACLLAQENIAPQDESFDPAVLKEPPLPMLD
                     NTLIYEVVTDLKPLTKPPVNNAADSIVYIEKMGWKVQVFSTSDYFLADSIYHEIRSQF
                     GDSTVEKVFNLPYYKIRIGNCATREEAERLLVRAQELGYRDAWVIRTQVKLAVKEGLF
                     "
     gene            complement(12001..12765)
                     /locus_tag="PKX09_07215"
     CDS             complement(12001..12765)
                     /locus_tag="PKX09_07215"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOU17647.1"
                     /translation="MKYLRLLILILSLTVGLIAENPAELDSLFEQGNRAYQEENYPEA
                     IRLYESIIARGYESGPLYFNLANAYYRVNNIGYAILNYERAARLMPNDPNVAFNLQLA
                     NLSVKDRVEAPPPFFLFRWYQNFLNLFSTRGWAVIFSILLLIAASSFAVWLNLDLRRL
                     RRGIKSLFIVSGALSIIVALILFQKYRIETASDFGIVLSGTVSSLSAPQAGSTELFII
                     HEGTKFKILDSDGNWLKIELVDGKQGWVLSADVAKI"
     gene            complement(12767..14596)
                     /locus_tag="PKX09_07220"
     CDS             complement(12767..14596)
                     /locus_tag="PKX09_07220"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOU17648.1"
                     /translation="MNRLKTFSLLFFLQLICLHALGQDLRISASVNQNNITDKDVLIF
                     SVTLEGISDFPEVPPPESPDFVLISGPSQSSSIQIINGKMSASKTVSWQIAPTRAGKL
                     MINPISIKYRGKIYSTEPVIVNVTASAPAQPRSAPPSGTPDKQVTPSSGEEVFLKAVV
                     PKTTIYMGEELIVSFDLYFKNVRTFGRKKLPDAQGFWKEELDAPTQPVITNETVNGVA
                     YRKATIQQIALFATTTGELTIDPMVVDCEVIQPSQRRSIFDDFFGGSFFDDPMFSKTK
                     IVTVQSEPIKIRVRPLPESGQPASFIGAVGQFKIESKIDTLQVKQDQALTLQYKISGS
                     GNINAIKLPPPSLPNSVEVFEPKIEKKIDKSSGSIKGSVTYEYVLIPRRPGLLTVPAI
                     WFSYFDPQTETYRTISTSSFKVKIVGSEDMLSDRSVGLNKTEVSLLGQDIRFIIRENP
                     VWRKSGQTVFSEFWFWLINGFSMLVIIGAITLRWWTEKMESNLTFARRRQAWSKWQNR
                     LRILQKMADNVVGEDFYAQLSQAVNGYIADRLGLPLAGLGPKEIEQELRRYAIDSELI
                     TKTVKLLEKLDEIRFLPGMIIADDPKEMLSSSREIVSALSKVI"
     gene            complement(14604..15326)
                     /locus_tag="PKX09_07225"
     CDS             complement(14604..15326)
                     /locus_tag="PKX09_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU17649.1"
                     /translation="MTRSKSISNCISRLSLFALGVVILCPLKISAADSGLRQYKKGNY
                     QEAIRAYDRILKDHDDWEEAHFGKGAALYKSQRIEEARREFEKAISVKDPMRKSAVYY
                     NLGNTLFQSGRIEESLAFYKKALEYNPRDFDAKYNYELARQILQQQKNQDKNQSEQKP
                     DQQQNQPEEQPQPDQQSQPQNKEQQEQQAQQQPSQQNRQEKEKSQKEAAQILDALKND
                     EKKLMQEKMRMKASGLAKEKDW"
     gene            complement(15333..16358)
                     /locus_tag="PKX09_07230"
     CDS             complement(15333..16358)
                     /locus_tag="PKX09_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOU17650.1"
                     /translation="MVKFYHSEFLWFWSLIILAILALIYNRIQHRKSLQQYAGIELSQ
                     KLLEGYRPGLRRLKNNLWLVGLVFLGIALLGPAVGQKLTEVKRRGQDIIIALDTSISM
                     KAEDVKPNRLERAKFELNRFIDGLMGDRIGIVAFAGTSYLQCPLTLDYSAAKLFLDAC
                     DTGIIGTQGTAIADAISTSLKAFKSGEKKHKVIILISDGEDHEGDINQIAKQAAEEGV
                     VIYAVGIGSLTGAPIPLRNITTGEVEFKKDRAGHIVMTALEEETMQKIAALTGGKYYN
                     LSANKDAFQKIAHEISGMDKKNLQSHEYTDYQQRYQIFLLIGLVLLTTEILIPEKRNS
                     ASRETNS"
     gene            complement(16358..17350)
                     /locus_tag="PKX09_07235"
     CDS             complement(16358..17350)
                     /locus_tag="PKX09_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOU17651.1"
                     /translation="MFYFANWGFLVLLILVPVLAIWYWKRGHKNAGALRYSSIKNILQ
                     VKNRHAFYKVHGPFVAKLIGIALLIMALARPQSGNTRREFTTEGIDIMLVLDISSSMK
                     AIDFRPNRLEAAKSVAQKFIEGRQSDRIGLIVFAAESFLQCPLTIDYGVLKDLLNQVD
                     IVEEKYDGTAIGMAIANATNRLRESQAKSKIMILLSDGCNNAGEIDPRTAANLANAFE
                     IKIYTIGAGKLGQAPYPIELPNGQIQYRLVNAEIDEVLLQDIANQTGGKYFRATDEKS
                     LAMIYEEISGMEKTEVKVHEFVNYNELYPYFLVPGFLLVALSGLIGLTIWRRTP"
     gene            complement(17369..18745)
                     /gene="gatA"
                     /locus_tag="PKX09_07240"
     CDS             complement(17369..18745)
                     /gene="gatA"
                     /locus_tag="PKX09_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOU17652.1"
                     /translation="MAGTVFLLGIEHLLERIKPNPYNAFISVAPDIAEKYDEINYKIQ
                     NGQAGSLAGYTIGIKDNICMVGLPTTCASRMLANFMTPYEATVVTKIRQADGLIVGKM
                     NMDEFAMGSTTEFSAFGLVNNPHNEKRVPGGSSGGSAAAVAGNLVDIALGSDTGGSIR
                     QPASFCGVIGLKPTYGRVSRYGLVAFASSLDQIGTFSRNVADSARLLQVIAGRDENDS
                     TTADEPVPDYQNALSKDVRNLRIGVPTEYFSKGLDPEIANRIKQCIQFLEKSGAIVTD
                     ITLPHTPYAIATYYIIATAEASSNLARYDGIRYGLSQRGGDLDTLYRETRHAGFGAEV
                     TRRIMLGTFVLSAGYYEAYYDKAQRVRRLIKEDFVEAFKKVDAILTPTTPTTAYEIGS
                     KIDDPLAMYLGDIYTVPANLAGVPALNFPLGKSSEGLPIGAQLIGRYFDEETILRIAH
                     YIERNYEE"
     gene            complement(18751..18978)
                     /gene="tatA"
                     /locus_tag="PKX09_07245"
     CDS             complement(18751..18978)
                     /gene="tatA"
                     /locus_tag="PKX09_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOU17653.1"
                     /translation="MGNIGTGEIILILIVFLLLFGAKRLPELAKSIGKSLREFKKAAS
                     DIQDEIKDVDSKPKNNSGNSTNPTEQPTPKE"
     gene            complement(18993..19268)
                     /locus_tag="PKX09_07250"
     CDS             complement(18993..19268)
                     /locus_tag="PKX09_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HOU17654.1"
                     /translation="MDSTKRKLSILFVIGILLLGAMIGSLLGELLRSLIPDGVVKEVF
                     LRSIDIMLGPAVIDLIMFSITLGFTLKLNFIGIIGLAVAYYILRFWR"
     gene            complement(19270..19956)
                     /gene="purQ"
                     /locus_tag="PKX09_07255"
     CDS             complement(19270..19956)
                     /gene="purQ"
                     /locus_tag="PKX09_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOU17655.1"
                     /translation="MKFGVVVFPGSNCDHDAYHVIKHVLGARVEYIWHKDTTLRLFDV
                     IILPGGFSYGDYLRAGAIARFSPIMPAIIQFAARGGKVIGICNGFQVLLEAGLLPGAL
                     IPNDHMRFRCQEVYLKVINKATAFTSEYSEGQVLKMPIAHYCGQYFADDDTLEQLQDQ
                     GRIVFQYCDSDGEVNPATNPNGSKLNIAGILNEDKNVLGMMPHPERAAEQVLGSVDGL
                     GIFKSLLGKD"
     gene            complement(19956..20210)
                     /gene="purS"
                     /locus_tag="PKX09_07260"
     CDS             complement(19956..20210)
                     /gene="purS"
                     /locus_tag="PKX09_07260"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOU17656.1"
                     /translation="MWLAKVVVSYKEGILDPEAKTIQKALKTLGYQNIEELSVGKYFE
                     IKLKSNLERSDVEKLVREICDRVLSNPVIQRYNYEIVETE"
BASE COUNT         5743 a         4612 c         4101 g         5802 t
ORIGIN      
        1 tagccgcaag tagttacgcc aggtcggctt caaattcgga gaagagtaga aatcccacat
       61 cacagcgcgc ggcggaaaca tttgccgcca taaataagca attttttgag acaaccttgg
      121 taaataaatc agcggcagta agattcgggc tcgcgccagt gaggtaaata gtcccccctg
      181 taagattacc tctgtattta ttttataaga caatcccgaa catcttactt taggatattc
      241 aaacaattgc ggtgagtcat tccacaaatc agatgaaacc cgagaaaaga tattccatac
      301 acgtgtcaag ttatatgtct tgattagatg ttgaaaatcg gctttatcga aatcaggcaa
      361 tttagtattt tgtcggcagt caataatcca aatcaactta aaataataat gcttaaacgc
      421 atgatatagc aaataaagag cttggtgaga tagatcgaaa gtttgatatt cacgatcgcc
      481 aatcttaatt attctggaat gcgaccaaaa gtcagcaaaa tcgaagtgaa acgggtgcaa
      541 gcgactgctt aagccagtat gcacatcaat cgccaccaat ggattcttta ccgaaatata
      601 atggcgggca aatctgtact tcttgatgat ttctgcagga aaattagtgt aaaggtcatc
      661 atccaactca caacctaatt cacctaatat cgtatgaact ttttccagtg attcaggctg
      721 aattaaaata tccagatcgc caaaagggcg ctgataaggg aattcataca attcataagc
      781 taaaactggt cctttgaaca aacggtattc gatgcttgcg gcccaaaata gttgatcgag
      841 attagccaga aatttttcat aaactataag tcggccggca ctttccagag cattaattcg
      901 gcatttttcg aaaaatgtgg ctgagatggc tgggaaaatt ttggatagag tcacacgata
      961 atcggcaatc agcggtagaa tctgatggta taatccgaga ttatagagtt tatcctcatt
     1021 gaaaacgaca ttatcaacgg caatttgatt ttcggtttca tctaaagcaa ccctacaaac
     1081 tgctcttaaa aacctacttt cagcattcat tgcttagaaa tatatacata accagataga
     1141 aaaacacata aaaattattt cagctaataa tcacgaaaag gaataaaagg ttattgaaag
     1201 ttagttaaac ggaagcctat ggaaaactgc cgattcagac cgcccgagca ttaaaaagat
     1261 cattgtcagg catcaatctt ttaagagcaa actcataata gaccggatta atctcgaagc
     1321 caatgtagtg acgtttcaac attttactta ctactgccac ctgccccgat cctaaaaatg
     1381 gatcgcaaac caaatcgtct tccgaactgg aatattgcaa gatttttttg atgacttcag
     1441 ctggtaattt ggtaggggtt ttctctttac cggtccaata ttcgcgctta atataccaga
     1501 catcttcttt atcgcggtaa tgcaaacttg agccagataa atcgcgggcg tctttatcaa
     1561 atcggctgta gggaaaaaat ttgcgctggc gatcattctt acagacgtaa agacaatgat
     1621 aatgggaagt gacatagcgc cgtctggtaa ctactccaaa ctgatactgc cagatcagat
     1681 gattgacggt cacgaaacca acttgatcaa gcgctatcaa cacttctttt aaattattcc
     1741 aaccggaaaa aacatacata ctcccgctcg gtttcaaaat ccgataagct tgagacatcc
     1801 atttgagcgt aaaattcaaa tattcatccg ctggaatttc agtataaccc tccaacactc
     1861 gcgacggagt tctatgataa ttattccgct gggctttgaa attaatcgca aacggtgggt
     1921 cagtaattat caaatcaaca aaatcctgcg gcatcttttg catcccgata acgcaatcaa
     1981 tattataaat atgatcaggt gccagtatta gttggaaatc ttccgattta gttcttcccg
     2041 acatagcaga ttatcagaat cgccaagtga acttggttgt caatcgacta gccagcctca
     2101 cataatcagg cttggtgatg atgtcttctg gtacgtgacg ccctcttttt cttggcgccg
     2161 gtatcgctat agtaagaata gtagcgataa tagtaatagt atgaatcata gccagttgac
     2221 atattcactc cattcagtac ggctccgact aggtttgctt ttacctgcct caacagttcg
     2281 attgatctct caagtactct cagatcggtc gaaccggctc taaccaccag taaaattgca
     2341 tcagtaagtc gtgatagaac agtcgcatcg gtaacagcga taaccggcgg agtatcaaac
     2401 aggattatat cgtatttttc tcgcgctctt tccataaaat ttttaagacg ctgagaggct
     2461 aagagttccg aaggattagg cggaatatca ccgcaagtta ttaagtcaag attgggaact
     2521 tcagtctggt gaataacctc ttcatccggc aagttctcaa ttaaaaggtg ggttaagccg
     2581 ggagctcgtt gaaattcgaa taccttatgt tgaatcggct tgcgcaaatc ggcatcaatt
     2641 agcagaactt tcttacccag attggcaaaa gtaatagcaa tattaatgac cgtggtggac
     2701 ttgccatcgc cggggccagc actggtaacg agcagactct tgattgggcg gtcgggactc
     2761 ataaactgga tattagtgcg tagagtgcgg taggcttcgg caacggccga cttgggatca
     2821 taatgggaaa ccaaccgcgc tttgaccagt acttcctctc cgttgttttt atccttataa
     2881 actttgccaa ttttctcgac agcgcttttg acatcaatgg tcggaatgat cgctatcgcc
     2941 gtcaatccta accgctgtaa atcctcaccc gatttaatcg aatgatcgag ataatcacgg
     3001 ataaatgcaa tcccgactcc cgcacctaat cccagaaata aacctaaaag cagattcatc
     3061 ttctttttgg gcttgaccgg tttttcgggt ggaatggctg gatcaataat gcgaacatta
     3121 ctaatcttac tggcttcggt aatccgcgat tcctgaagtt tcgttttcat caggatatag
     3181 tacttttcat ttacctgacg ttcacgatcc aggcgggcat attcgagagt actggctggg
     3241 agtctatcaa gtcgggcaga atactgatca actaattttt tatattcatt acatttggct
     3301 tccagggaaa tgacctcgat attgttcatt attaccttat tgagtagttc agccgaaatt
     3361 ttagattgct cttcgggcgt caatcctaaa ttggtaatct tttcagcttc ttcaaatagc
     3421 ttctgcttta aatctgtaat tcttttatcc agctctttga ttgccgggtg gtcagcacta
     3481 taaccttgaa cggtcattac tgtaattttt tcggcttcca tttcggcaat actttgacga
     3541 agttgagcta cgaagggatt agtggtattg accgattcct ggataaccat ttgctcgcgt
     3601 tcggttagca ttctctgttg attatcaaga gtttgctgag ctattttcag atcagctaga
     3661 gttgtataat aagtagcctc aaaagtgctg agttgatcaa ttaaaatttt agcagtttca
     3721 tccagaccga aaataccttc ttggcgctgg taatttttca aagcctcttc cgactctttc
     3781 aggtcctttt ctattttttc gagttgttcc tcgaggaagt ttttcatctc gatcacttcc
     3841 ccaaggttgt attctacatc ggtcttataa tattcacttg cgatcgtatt agcaattagg
     3901 gcggcttcta tgggatcaac ggaagaaatt gtcaaccgaa tagcttcagt ttcgcgaatc
     3961 ggttcgactt tcatagatgt cattaaattt ttgacaatag ctcgatcgag ggtatcaact
     4021 accatcaaat cttcctcttg ggacggcata aaaatatgct gtaatccgct ggttatgccg
     4081 cccatcagat ctcctaagcc aacgcctttt ttatggtatt ttcttgtttc taaaaggtat
     4141 agactgtcag cataaggcga attacgtaag gctttgatta catttcgcgc tagagcagtg
     4201 cttttcaaca cctctatctc gttattgact ttcatgtaac tcatcggtaa caatggattc
     4261 tggaataaag tgctggtaga agcggcgcgg ccggagagca tcagagtcgt agatgcctga
     4321 tattccggcg gagtggtaaa agtaatgtat attgtgagga gcataaccac gacgaaaacg
     4381 attccgatga tccagcggcc tcgataaata atccgaaaat aatcctgaag cgaaacttcc
     4441 ttttctgttt cagtaaagct tctttccgga attcctgttt tgatattacc attcatagtt
     4501 tccattgagt aatcctatgc tcctttctca acatacactt cgactgtact cttgaaattt
     4561 aattgatatt taagaaaata aaacaaaccc aaagtggtta gactaataaa accgactgcg
     4621 tgcataatta ccgccacagc ttgagctttc gacaattcta accctagaaa aaccagaatg
     4681 ccgatcgtcg cggcatgata ggttcccacg tagccgggag ccgacggaac tgaaatgatg
     4741 gcagaagtaa caaaaaaaac cagcaataaa tcgaacaaat cgaagtaaac ctgtaaggac
     4801 gcgcccatca accagaaatt gccgatatta accgcccaga tcaagaacga ttgaaagata
     4861 atcaatccca gatgcggcgt ttctcgcaag gtcgtcaaac cttgccaaaa attcttaacc
     4921 gtcttgccta ttttactatc atctaaattt tttatcttta gcatcttttg cttgaagaac
     4981 cttttcagtg caactttttt aaggtttgaa atatatacca aaataaataa aattaaaata
     5041 actattccac taattaaacc gcttgttcga acccatttag gcatacccgg gaaaaaaata
     5101 gctccactga ttagaactat aataaaagtc agaacatcga gagtccgctc caatacaata
     5161 gtcccgaata cggcgctaac cggtatattt tgggttttac tcagagcata acccctgaga
     5221 atttctccca tacgaaaggg aaaaacatgg ttaccgaaat aaccgatcat ctccatctca
     5281 aaaacctgtt taacaggcaa atcatttatc ggtaaaatca aatatttcca gcgccaaccg
     5341 cgtatcccga ttagaaaaac cataaccgcc attgacaatc caaaaatcca gaggttggtc
     5401 agacgcaagt aacttaaaaa ttcggtgcta tcaaatttgc ggaagcccaa ataaagtccc
     5461 aaaatactga tggcaaaggc gaataacgtc ttcggactaa tgagtttttt ccaaatatta
     5521 ctggagttat ttttcatggc cgtaaatcta ttagttcatc gccttcattt aaattaattt
     5581 tgaataatcc ggtatcggct cgccccaaag cgatcagagt atctaattca atattaacca
     5641 aattatcttg aaaatcacga tattcagccg atactatcct tccgcgttca cgatcaattt
     5701 tcatctgtaa ggaacggata cagccatctt tagaagacgg tttcaattct acaacagcta
     5761 aaagaccatt ttggcactca cttttactaa tctgataacc gtcgaaggaa ccacttaaaa
     5821 tatcccaaat caattttaga ctggaacgcg gattgaattc ttcaactatt acttgccgat
     5881 taactttatc gtaggtccag agccattgac catcatacac gatttcctct tccgtttgac
     5941 tcacgcgaaa ctggttatca gagcctagaa aaaagatacc gctgtattta ctcaaattgg
     6001 cattgtccgg cattgccagt cggaccgtaa acccgatttt aaacggcacc gctgattgat
     6061 aacagtgcgc gaccttttta agaatcacct gagtggtttc ttgagctaat aataatattg
     6121 gaatagtcag taaaacagac aagattatag aattgaaaat atatttacga gttaccaatt
     6181 tcacggcaat aatttaagaa gttttcagtt agtagctttg atatttatca cagaatttca
     6241 gacctcgtct tcgtcatttt gaattttatt caattgctcc agataatctt catccactaa
     6301 aacttcgcga gctttactac cggtaaacgc cccgacgatg ctggcttttt ccagttcatc
     6361 aattaaccga gcggctcggg aataaccgat ttttagtcgg cgttggagaa gagaaatcga
     6421 accttgctga tgagttacga ccaaacgcaa agcttcataa tagagcgggt ctttttcgct
     6481 gtcatcgtat aagccgaatt cttctacgct ttcttctttt accgagggta attcgacctg
     6541 acccggtcgg ggttggttct caatatgctc cagaattcgt tcaatttctt ccagtgaaat
     6601 ataagcgctg tgaactcgga aaggctcggg attttgaggt gtggtaatta acaaatcgcc
     6661 gcggcctagt aatttttcgg caccatttaa atccagaatc gttcgcgaat caatttttga
     6721 agccacttga aaggcgatac gagcagggaa attagctttg ataacgccgg tgatgacatc
     6781 aactgacggg cgctgggtgg caataatcaa atgaataccc accgcccgtg ccatctgagc
     6841 taagcgcgta atcggttctt cgacttcttt ggctgaggtg agcatcaaat cggcaagttc
     6901 atcaattaca atgataatat acggcaggaa ttctttatcc agttcaccac tgaccacccg
     6961 gttattatat tcatcaatat tgcgtacggt agcactcgcc agaatttcat agcgccgttc
     7021 catttcaagt tcgacggaac gcaaggctac aatcgcatta tccgcccgag taataacgtc
     7081 ttcatccaga tcctcacaag ttattaaatg ataaccttcc agggctttga aaatggacaa
     7141 ttccaatttt ttaggatcaa tcagcataaa tttgacttca tccggtcgag ctaaataaag
     7201 aaggctggcg ataatagtgt taatgcagac acttttaccg gagccagttg caccggcaat
     7261 tagcagatga ggcatacgcg ccaaatcgag agtataaatt tcaccagagg tggtttttcc
     7321 gagagcgatg gtcagtttcg acttagcact ggcgaattgc cgtgagccga ttatactgcg
     7381 cagatagaca atttccggat tacggttggg aatctcgacg ccgaccaccg atttacccgg
     7441 aatcggagct acgatccgga tgcgtttagc ggaaagaatc cgcgccagat catcggctaa
     7501 ggcggcgatt ttactaaccc gcacgccgac ggcaggttca atttcatatc gggtaataat
     7561 cggacctggc gaaacgttga ccaccttacc ttttacgcca aaggtttcca gtgcattttc
     7621 cagcaactgg gcattttcag ccatctcttc cttagtcacc gaatctcttg actctggtgg
     7681 taattcgagt aaatcaatag gcggcagttg atatactccg cctgaggtct cagatttagt
     7741 tactggttcg acttttggtt cgattattgg cggttgtggt tcaaccgact ttttaatttt
     7801 gactggtcga gtagcttcgg tttcagattg aacaactgcg gcatcctttt ccgattgcga
     7861 tacagaaaac tcccgttcag gaatacgagg ttgaggtatt tcgccggatg ttctttcctt
     7921 ttttgggtga cgatgcgaac gccatttagc gatcgggcta taaaaactcc aagtaaaata
     7981 gccgcggatc gtcaccaaag ccagcactaa gagtacaatc accgtgccgg taaaccccag
     8041 ccaatcacac attaccttgg caaagactcc gccaaccaag ccactatagc ggaaaccgaa
     8101 gttactgtcc acattttctc taacagccgg tagtcccaga gctaccgata ctaatagagc
     8161 cataaaaata gaatataaac tgaaccggat taatatcttg cgttccttat cggcaaataa
     8221 ccaccagccc cagaccaaca tcaataccgg aaaaatgaaa accgcaactc cgagggtgta
     8281 tttaatcaaa taatgggaaa tatatactcc cacaattccc atccagtttt ggatagcgac
     8341 atttttcgag atagtcggtt cttcaatcgg attgtaggaa atcaagctca gcataattaa
     8401 aataccgagt gcaataagta aaatgccgag tatttcctgc tgtcggttat tcactgcgtt
     8461 ctcgctggga tgaatggggt ttttacgatt acagctttta gtaatttgcc gcgaatttcg
     8521 atttcaatcg gcgttccgac agcactatat tcggaagcga cataggcaat tccgattcct
     8581 ttgtttaaag tcggcgactg ggttccactg gttactagac cgatttcctg gccattttta
     8641 aaaaccttat agccatggcg cggaatacct ggctcgagca tttcaaagcc gacactttta
     8701 cgttttaagc cttccgcttt tattttgagt agcgctgctt ttccgatgaa atctggtttg
     8761 tcaagtttag taatccagcc cagtccggct tccagcggat tagtcgtctg atcaatatcg
     8821 ttgccataga gtgcatattt catttcgagg cggagagtat cacgggcacc taatccaatt
     8881 ggttggatat cgtattttgc cccggcttga aaaatagcct cccaaagtct ggcactgtca
     8941 gccggtttat gataaatttc cagccctttt tcgccggtat aaccagtccg cgacaggata
     9001 gccggaacgt ttgctatctc accttcccga aaatgataaa attcaatttc atttaaattg
     9061 attggcgtta atggttgtaa ggtctcaagg gctaacggac cttgaacagc cagaagtccg
     9121 gtttcatcgg aaatatcagt caattccact cccgcaggaa gatgttgctt gatccagtta
     9181 aaatctttct ccagattggc ggcattgacg accagcataa aatgatcagc aaaacgataa
     9241 acaagtaaat catcaacgat tccaccgtcg ggatagcaca ttgcagaata ttgcacttga
     9301 ccgattgcta atttggacac atcatttatc gtaatgtaat tcaaaaagtc ctcagcggag
     9361 gaacccctga cgatgaattc acccatatgg gaaacatcga aaactccgac tgttgttcgg
     9421 acgcggcggt gttcttccat aataccgcga tattgaatcg gcatccagta accggcaaaa
     9481 ggcacaagtt tggcgttaca agccaagtgc tggtcataaa atggggtctt ttttacctga
     9541 ttgttagcgc tttccatatt tccgccttac atttgattaa gagccgcttt gatatatttt
     9601 tcaacgggcg cagagtcacc gagttccttg acagctttgc gtagagcatt cagagcttct
     9661 gcccgccggt aaccgagcga aatcagggcg cgtaatgctt cctcgccagt cgctcccaaa
     9721 gtaacagtcg caagttcacc ggaaacaacc tcagatgtct gtccaatttt ctctcccaat
     9781 tcaacgataa ttcgtcgcgc cgtttttaat ccaatacctg gaattaaggt tagagattcg
     9841 acatcaccgt ctaatatccg acgccgaaat tgttcgggac tagtgccaga gagaatcgtc
     9901 attgccatct ttggtccgat gccggatacg ctgattagca gtaaaaacag ctctttttcc
     9961 tcgggagtag caaagccata caaagcctgg atatcttcgc gaacatgata gtaagtgagt
    10021 aacttggcat tttgaccggt agctggtaga gcggaatacg ttgataaagg aatattaatc
    10081 tgataaccaa taccgttgac atcaattatg accgaagtag gctctttctg gaataatttt
    10141 ccactaatat aagcaaacat actttaatcc ctaatgtttg gacggttaag gtgacagatt
    10201 gctgccgcca tagcatcagc ggcatcgaag gattccggtt cttgcgagag gcctaaaatc
    10261 cgactaacca tatattgcac ctgttcttta gatgctaaac cgttaccaac tacagccgat
    10321 tttattttgg tgggtggata ttcggcaatc gtcaagccgg cattggcgcc tgccagtaat
    10381 actgctcccc gtgcatgtcc catcagaagt gcagttttga tgttctgggc atagatagct
    10441 tcctccacta ccatgataga cggtttccaa gcttcaataa tctggagcaa ttccgtataa
    10501 atgtgtttta aacgcagatt aaaggcttca cgaggagtag tcacgattga gccatgggta
    10561 agacaagaga gcttagagcc gtttttacta acgatagcgt acccggtgcg attgatacca
    10621 tggtcaacgc cgattactct aagttcttca gacattatcc ggcactctgt tgcaattcag
    10681 ccagtaattt atcatcaata tcgaagttgg cgtaaacatt ttgaatatcg tcatgatcct
    10741 cgagagcatc cattagactt atgattttcg gagcgttttt ttcatctact ttgatagtag
    10801 ttttaggaat catggttacg gcggcgctgg caatggcaat cttagccgat tccagcgctt
    10861 tttttacctt ttcaaaatga ccgggatcaa cgctgacttc ataaacgtcg tcttcagtgc
    10921 taatatcgtc ggcgccagcc tccagagcaa tcaccagaag atcgtcttcc gtgcaagtat
    10981 ctttattcac ggtaatgata cctttctttt caaaattata ggcgacactg ccggtggtag
    11041 ctaaattccc gccgtggcgg gttaaaatgt ggcgcagttc agaaacggtg cgatttttat
    11101 tatcggttaa ggcttccatt agaattgcca cgccgccggg tccgtaggct tcataagtga
    11161 tttcttcgta attgacgctt tcaatttcac cggtgccttt tttgatggct cgttcgacat
    11221 tagctaaagg catattagcc gctttagccg ctaagaccgc tgtgcggagg cgcggattag
    11281 cattttcatc gccgccgccc attttagcgg cgacttgaat ttccttaata attttagtaa
    11341 aaaccttgcc gcgcttggca tcgagagccc ccttttttcg cttaattgtg ctccattttg
    11401 aatggcctga catatttaaa acctcatcat ttttaatatt ttgttaaaat aagccttctt
    11461 taacagccag cttaacctga gtgcggatta cccaggcgtc ccgatagccc aattcttgag
    11521 cccggactaa caggcgttcg gcttcttccc gcgttgcaca gtttcctatt cggattttat
    11581 aataaggcaa attgaacact ttttcaactg tcgaatcacc aaattggctt cgaatttcat
    11641 ggtaaattga atcagccagg aaataatcac tagtggaaaa cacctgaact ttccagccca
    11701 ttttttcgat ataaacgata gaatcagccg cattattaac tggcggtttg gtcaacggtt
    11761 taagatcagt aacaacttca tatatcagcg tgttgtccag cattggtaga ggcggctcct
    11821 tcaaaacggc gggatcaaaa ctctcatcct gaggtgctat attttcctga gctagcaaac
    11881 aagcaaatga taataaaatt aaaactacta tggctttatt tttcatattc gtaaaattaa
    11941 cacagccaaa atatacggag attatgggca attttaaaag agataaacag tcttaggcgt
    12001 ttagatttta gccacatcag cgctcagtac ccagccttgt ttcccatcaa ctagctcgat
    12061 ttttagccaa tttccatcgg aatcaagaat tttaaacttg gttccttcgt gaatgataaa
    12121 taactcagtg ctaccggctt gaggggcgga tagactgctg accgttccac ttagaacgat
    12181 cccaaaatcg ctggcagtct cgattcgata tttttggaac aggatcaaag ccactataat
    12241 actgagcgca cctgaaacaa taaaaagtga ctttattccg cggcgcagac gtcttaaatc
    12301 cagattcaac caaacggcaa aactactagc tgcaattaag agtaaaatcg agaatatcac
    12361 cgcccaacca cgcgtactga atagattcaa aaaattttga taccaacgga aaaggaaaaa
    12421 tggcggtggc gcctcgactc ggtctttgac actcaaatta gccaattgca ggttaaaagc
    12481 cacgttcgga tcattgggca taagtcgggc ggcgcgctca taattcagaa tcgcataccc
    12541 gatattattg acgcggtaat aagcatttgc caggttaaaa tatagtggac cgctttcata
    12601 gccgcgcgca atgatgctct catacaagcg aatggcttcc ggatagtttt cctcctggta
    12661 agctcgatta ccttgttcaa acaatgaatc gagttcggct gggttttcag ctattagccc
    12721 gacggttaaa cttaaaatca gaatcaatag gcgcaagtat ttcattttaa atcactttac
    12781 tcaaagccga tacaatttca cgactggaag atagcatctc ttttggatca tcggcgatta
    12841 tcatccctgg tagaaagcga atttcgtcca gcttctcgag caattttacg gtctttgtta
    12901 tcagttcaga atcaatggcg taacgtctca attcttgttc aatttccttg gggcctaaac
    12961 cagccagcgg taaaccgagc cgatcggcga tataaccgtt cactgcctga gacaattgag
    13021 cgtaaaaatc ttcaccgacg acattatccg ccattttttg caaaatccgt agacggtttt
    13081 gccatttcga ccaggcttgt cggcgtcgcg caaaagtcag gttgctctcc attttttcag
    13141 tccaccagcg cagagtgatt gcaccaatta taaccagcat cgaaaagcca ttgatcagcc
    13201 aaaaccagaa ttcgctgaaa acagtctgcc cggacttacg ccagactggg ttctcccgta
    13261 taatgaaacg aatatcctgc ccgaggagtg aaacttcggt cttgttaagc ccaacgcttc
    13321 ggtcagaaag catatcttca ctcccaacga ttttgacttt gaaactgctg gttgatatag
    13381 ttcggtaggt ttcggtttgc ggatcaaagt aagaaaacca aatcgctgga acagttagta
    13441 atccaggccg acggggaatt agcacatatt cataagttac cgagccttta atacttccgc
    13501 tcgacttgtc tattttcttt tcgattttag gttcaaaaac ctcgacggaa ttgggtaaac
    13561 tgggcggtgg taatttgata gcattaatat taccactgcc actgatttta tactggagtg
    13621 tcaaagcttg atcctgtttt acctgcagag tatcaatttt gctttcgatt ttaaattgcc
    13681 caactgctcc gataaatgaa gcgggctggc cgctttcggg aagcggtctg actcgaatct
    13741 taatcggctc cgattgaact gttacaatct ttgttttcga aaacattgga tcatcgaaaa
    13801 aagaaccgcc aaaaaagtcg tcgaaaatcg aacgccgttg actaggctgg attacttcac
    13861 aatcaaccac cattggatca atggtcaatt ccccggtagt agttgcgaaa agggctattt
    13921 gctgaattgt cgccttacgg taggcaacac cattgacggt ttcattagtt ataaccggtt
    13981 gagtaggcgc gtccagttct tccttccaga atccctgagc atcgggtagt ttcttacggc
    14041 cgaaggtgcg cacattctta aagtacagat caaatgaaac tattaattct tcacccatat
    14101 atatcgtagt tttaggaaca acggctttta ggaaaacttc ctcgccgcta ctcggagtca
    14161 cttgtttatc gggagttccc gaaggtggtg cggaacgggg ttgggcaggc gcggaagcag
    14221 tgacgttaac aatgaccggt tcagttgaat agattttgcc gcgatatttg atcgaaatag
    14281 gattaatcat tagtttgccg gcacgcgtag gtgcaatttg ccagctgacg gttttcgagg
    14341 cactcatctt accattgatg atttgaatac tgctggactg tgatggtcca gaaatcagaa
    14401 caaagtctgg actttcagga ggcggaactt ccggaaaatc actaattcct tccaacgtaa
    14461 ccgagaaaat tagcacatct ttgtcggtaa tgttgttctg gttaaccgag gcggatatgc
    14521 gcaggtcttg gcctaaggcg tgcagacaaa ttaactgcaa gaaaaacagt aagctgaagg
    14581 tttttaatct attcatccag gaattaccaa tctttttcct tagccagacc agaagccttc
    14641 attcgcattt tttcctgcat tagttttttc tcatcattct tcaaggcatc cagaatttgc
    14701 gcggcttcct tttgactttt ttctttctct tgccgatttt gctgagatgg ttgttgttga
    14761 gcctgttgct cttgttgctc tttattttgc ggctgagatt gttgatccgg ttgaggttgt
    14821 tcttctggct gattttgttg ttgatccggc ttttgctccg actgattttt atcttgattt
    14881 ttctgttgtt gcaatatttg ccgagccaat tcataattat atttagcatc gaaatcgcgg
    14941 ggattgtatt cgagggcttt tttataaaaa gccagacttt cttcaatacg gccactctgg
    15001 aataaggtat ttccgagatt ataatagact gccgattttc gcattggatc tttgacggaa
    15061 atagcctttt caaattcacg cctagcttct tcgatacgct ggcttttata aagggcggcg
    15121 cctttgccaa aatgggcttc ttcccaatcg tcgtggtctt tcagaatacg atcataagcg
    15181 cggattgctt cctgataatt gcctttttta tactgacgca gaccagaatc agccgccgat
    15241 attttcagcg gacataagat aaccactccc agggcaaata aacttaaccg acttatacaa
    15301 tttgaaatgg atttagatct cgtcatattt gttcaagaat tagtctcacg tgaggctgag
    15361 tttctctttt ccggaattaa aatttcagta gtcaaaagca caagaccaat taataaaaat
    15421 atctgatagc gttgctgata atcggtatat tcgtgcgatt gcagattctt cttatccata
    15481 ccagatattt cgtgggcaat tttctgaaag gcatctttat tggcactcag gttataatat
    15541 tttccgcccg tcaacgctgc aattttttgc atggtttcct cttctagggc agtcattacg
    15601 atatgtccgg cacggtcttt cttgaattct acttcaccag tcgtgatatt gcgcagaggt
    15661 atcggtgccc cggtgagcga accgattcca acggcataaa taactacacc ttcctcagcc
    15721 gcctgcttgg cgatttgatt aatgtctccc tcatgatctt ctccatccga aataaggata
    15781 atgactttgt gctttttttc gcccgattta aaggctttta aactagtact aattgcatca
    15841 gcgatggcgg ttccctgagt accaattatg ccagtatcac acgcatcgag aaataactta
    15901 gccgcactat aatccagagt cagaggacac tgcaggtaac tggtgccggc aaaggcaact
    15961 ataccgattc gatcgcccat caaaccatca ataaagcgat tgagctcaaa tttggcgcgt
    16021 tccagacggt tgggtttgac atcttcagcc ttcatactga tggaagtatc gagcgcaata
    16081 ataatgtcct gtccgcgtcg tttgacctca gtcagtttct gaccgaccgc cggtcccagc
    16141 aacgcaattc ccaagaaaac cagtccgacg agccaaagat tattttttag acgccttaat
    16201 cccgggcgat aaccttctaa caatttctga gaaagttcga ttccggcata ctgttggaga
    16261 ctcttacgat gctgaattcg gttataaata agcgccagta tcgccagaat tattaggctc
    16321 cagaaccaga gaaattccga atgataaaac ttgaccatca gggtgtcctc cgccagatgg
    16381 ttaatccgat caacccgctt aacgcaacca ataaaaagcc cggcactaaa aagtaaggat
    16441 aaagttcgtt ataattgacg aattcgtgaa ccttgacttc ggttttttcc attccgctga
    16501 tttcctcgta aatcatcgcc agactttttt catcagttgc ccggaaatac ttaccgccag
    16561 tttgattagc aatatcctgc aaaagaactt cgtcaatttc agcatttacc agacgatatt
    16621 gaatttggcc attgggcaat tcaatcggat aaggcgcttg ccctaatttt ccggctccaa
    16681 tcgtatagat tttgatctcg aaggcgtttg ccagattagc ggcggtgcgc gggtcaattt
    16741 caccggcatt attacagcca tcggacaaga ggatcattat tttgctttta gcttgacttt
    16801 cacgcagacg attggtagca ttggcaattg ccatcccgat cgccgtccca tcgtattttt
    16861 cttcgacaat atcaacctga ttcagcaagt ctttcaagac cccataatca atggtcagtg
    16921 ggcattgcaa aaaactctcc gccgcaaaaa cgattaaacc gattcggtca ctttgacgtc
    16981 cttcgataaa cttttgggca accgatttag cggcttccaa ccgattgggg cggaaatcaa
    17041 tagccttcat cgagctggat atatccaata ccaacattat atcaattccc tccgtggtaa
    17101 attcccgcct ggtattgcca ctttgaggcc gagccaacgc cattattagg agagcaattc
    17161 cgatcagttt ggcaacaaac ggaccatgca ctttataaaa ggcgtggcga tttttaacct
    17221 gtaaaatgtt tttaattgac gaataacgca gagcgccggc atttttgtgg cctcttttcc
    17281 aataccagat agccaagacc ggcacaagaa ttagtagaac taaaaaaccc cagtttgcga
    17341 aatagaacat acagcttaat ctggtaggtt actcctcata atttcgctct atataatgag
    17401 caatccgtaa aattgtttct tcatcgaaat agcggccaat caattgggcg ccaatcggta
    17461 atccttccga cgatttaccg agtggaaaat tcaaagccgg cacgcccgct aaattagccg
    17521 gtacggtata aatatcaccg aggtacatag ccagcggatc gtcaatcttg gaacctattt
    17581 cgtaagcggt tgtcggtgta gttggcgtca gaatcgcatc tactttctta aaagcctcta
    17641 cgaaatcttc ctttattaaa cgcctgacgc gctgagcttt gtcgtaatat gcctcataat
    17701 aaccagctga caaaacgaat gtaccaagca taatgcggcg agtcacttct gctccgaatc
    17761 cggcatgtct ggtctcgcga tatagagtat cgagatcgcc accgcgttga cttaaaccat
    17821 atcggatacc gtcatagcgt gccagattgg aagaagcttc ggcggtagca atgatataat
    17881 aagtggcgat ggcataaggc gtatgcggca aagtaatatc ggtgacgatc gctcctgatt
    17941 tttccaaaaa ctgaatgcac tgcttgatgc ggttggcaat ttcgggatcg agtcccttgc
    18001 taaaatattc ggtcggcacg ccgatgcgca gattgcggac atctttactc agcgcattct
    18061 gataatctgg cactggttca tcggcggtcg tcgaatcatt ctcatcgcgt ccggctatca
    18121 cctgtaacag gcgtgcgcta tcggctacat tgcgcgaaaa agtcccaatc tgatccagag
    18181 acgaagcaaa agctaccaac ccatagcgcg acactcggcc ataagttggt ttaagaccaa
    18241 taacgccaca gaaagaagcc ggttggcgaa tcgaaccgcc cgtgtcagaa ccgagagcta
    18301 tatccaccag gttcccagct accgccgcgg ctgaaccacc actggaccca cccggaactc
    18361 gcttctcatt atgcggatta ttcaccaagc cgaaagccga gaattcggtt gtagaaccca
    18421 tcgcgaattc gtccatattc attttaccga caatcaatcc atccgcctgc cgaattttag
    18481 taacgaccgt ggcttcataa ggcgtcataa aattagccag cattcgcgaa gcacaggtcg
    18541 tcggcaaacc aaccatacag atgttgtctt taattccgat ggtatatccg gctaatgagc
    18601 cggcttggcc attttgaatc ttataattta tttcatcata tttttcggca atatccggcg
    18661 caactgagat aaaagcattg tacggattag gcttaatccg ctcgagtaaa tgctcgattc
    18721 ctaatagaaa aactgtacca gccacccgtt ttattccttg ggtgtcggtt gttcagtcgg
    18781 attggttgaa tttcccgaat tatttttcgg tttggaatct acatccttta tttcatcttg
    18841 aatatcgctg gcggcttttt tgaattcgcg taaactcttg ccaatacttt tggccaattc
    18901 aggtaagcgt ttagcgccaa acaaaagcaa gaaaacaatt aaaataagaa ttatttcgcc
    18961 agttcctatg tttcccattt tgactccttt agttatctcc aaaatctcag gatataatag
    19021 gctacagcca gcccaattat cccgataaaa tttaatttca aggtaaagcc caacgtaatt
    19081 gaaaacatta tgagatcaat tacagctggt ccaagcataa tatcaattga ccgtagaaaa
    19141 acttctttga ctactccatc gggaattaac gagcgcagta attcaccgag cagagaaccg
    19201 atcattgcac ccagtaatag aataccaatc acgaataaaa tcgaaagttt gcgttttgtc
    19261 gaatccatct taatctttcc ctagcaaaga tttaaaaatc cccaatccgt ccactgaacc
    19321 gagcacctgt tccgccgccc gttctggatg cggcatcatt cccagtacgt tcttatcttc
    19381 attcagaatg ccagcgatgt taagcttcga tccattggga ttggttgccg gattaacttc
    19441 cccatcagaa tcgcaatact ggaaaacaat tcttccctgg tcttgaagtt gctccaaagt
    19501 atcatcatcg gcaaaatact gaccacagta gtgggcaata ggcattttta aaacttgccc
    19561 ttcactatac tcgctggtaa aagccgtggc tttattgatt actttaaggt aaacctcctg
    19621 acagcgaaat cgcatatgat cgttaggaat taaagccccg ggcaatagcc cagcttccaa
    19681 cagtacctga aaaccgttac atatcccgat gacttttccc cctctggcgg caaattgaat
    19741 aatggctggc ataatcggtg aaaaacgggc tatcgctccc gcccgcaaat aatccccgta
    19801 agaaaaaccg cccggcaaaa tgattacatc gaaaagtcgc aaagtagtat ctttatgcca
    19861 gatatactca actctcgctc ccagaacatg tttaataacg tgataagcgt catgatcgca
    19921 attcgagccg gggaacacca ctactccgaa ttttatcatt ccgtctccac tatttcgtaa
    19981 ttatatctct gaatcactgg attagaaaga acgcggtcgc atatttcccg aaccaatttt
    20041 tcgacatcgc ttcgctcaag attggatttc agctttatct caaaatattt accgactgac
    20101 aattcttcga tattctgata tccgagagtt tttagagctt tttgaatcgt tttagcctcc
    20161 ggatcgagaa taccctcttt gtaagacact accactttcg ctaaccacat ttaatgcgcc
    20221 tttcttctgg ctaaatgaat acggggttca tcatagcc
//
LOCUS       DAOSMP010000038        19805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_2727_length_19805_cov_19.837266, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000038 DAOSMP010000000
VERSION     DAOSMP010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 19805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19805
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2727_length_19805_cov_19.837266"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2292)
                     /locus_tag="PKX09_07265"
     CDS             complement(<1..2292)
                     /locus_tag="PKX09_07265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOU17657.1"
                     /translation="MPDSIKETNDKLGFAKIIELIQEMALSTAGKSLANTILPLKDIE
                     MIRCRQSRINALQAILSAGDSLPLNAFSDFRAELDRCRVIGTFLSLETLIQIDQLLEL
                     VERQRRFFRSQRPQLLSLEELFEGLDSLPQVRKTIAAIIAPDGSLRDSASPQLNRIRH
                     EIRTHQRRLNHEIERLMNYARDQQWLHEDQATIRDGRLVLPLRVESKRKINGVIHGQS
                     ATGATVYVEPLEIVEINNALKELELAEKEEIERILRQTTDKMRPDFSPLQKNIQILAE
                     LDLYNACARFGQKFKCTPPIIAENNNFVLHQARHPLLALHKEVVPLDFELANDFRAVV
                     ISGPNAGGKTVAMKTVGLLSQMAMSGLPIPAEEESRLPLITQFLADIGDQQSLENDLS
                     TFSSHINNMKYFLETADDRTLVLIDELGTGTEPTEGAALGQAILTELIAAGSLIIVTT
                     HHNALKEFAQKQPGAINAAMEFDMVTLAPTYRLQIGLPGSSYAFEISNRLGLPTKVVD
                     NARQIVGEESVRLTNLLREVEQLRSEVEKDKKIISQNKKTLDKLVTEYNNKLNTLREK
                     AERADTELARQLEELVNESRRRIENVVKEIREQAATPETIHKAQSTITEIQTNVRKIK
                     ERSKRAAPVAQVEFQIGDTVRIDGISEPGEIIKIGSQNSRFGVAVGGKILWVSRTVLH
                     PFNKSTASRKQAAIVSVESEPLFSPRLDLRGMRYDEAESALQKFLDRALLAGLNQVEI
                     IHGKGTGALQKLTQEVLRTTPGVR"
     gene            complement(2303..2881)
                     /locus_tag="PKX09_07270"
     CDS             complement(2303..2881)
                     /locus_tag="PKX09_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOU17658.1"
                     /translation="MFNLIVAVIIGVCIFFGLRNGLIKSAFKLIGLVLAALSASRYYY
                     FGGKIIADLFNLSEGVQSVIGFVIVFIVVFLLFELLASMLRSLLRALKLIWVDRIGGM
                     LFGFLEGAIIMIGIVWVLNVYPELGFVDHLRQSSANFKFFERSGEKVIKICGLNSEMD
                     HLRKSMRKIVFLPETSETNPKDTNISPSKNNP"
     gene            complement(2895..3671)
                     /gene="pgeF"
                     /locus_tag="PKX09_07275"
     CDS             complement(2895..3671)
                     /gene="pgeF"
                     /locus_tag="PKX09_07275"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOU17659.1"
                     /translation="MFPLTVSKGIEIIQSPELMTFPEIVHAFSTRNGGVSNGPFRSLN
                     LGIADGDTLKNILINRKRFYNTLNFTSSDVAQAEQVHSNKIAIVEDNGPIPGCDALIT
                     TQANLYLSIKTADCAAVLLYDPHHQVIAAVHAGWRGTAEGIIANTINTMQAQFQTNPG
                     ELIAAIGPALHVCCYKVKNNVAKKFSDNEIIQREEGLFLDLILAITNRLNAAGVQLDR
                     IADSGYCTACQPEKFYSYRRDEGVTGRMLAVIGINQRNKC"
     gene            complement(3671..5680)
                     /locus_tag="PKX09_07280"
     CDS             complement(3671..5680)
                     /locus_tag="PKX09_07280"
                     /inference="COORDINATES: protein motif:HMM:NF016251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HOU17660.1"
                     /translation="MMKLPRTATFILVISLIWGSSLLADQTTDKLRSLLDEGIACYNA
                     EKYWDAQNIFKIINSIPKDENPYLTTSALMLIKTTYRLGEAEKSIALAYDFLQNYPES
                     KYVKEVKLTLAEVQLSLGNYQESLRIYLDLLKNYTDQEIQSQCEDYLNKIIDFYLEAE
                     TVNALRDSTSDNFSRQYLTIKLAEKYHSEGHTDLAEAELKRLPARLENKYLRDQLQKV
                     EVQLKKPSVVRKYIGVILPLSGSNSASGREILNGVKYAIANYRKNNSEDIVALVFDNR
                     SDGVLSIKQAEFLARSPKVLAIFGPLMTENAVLIAVIANQYQIPMITPTASGSQLASL
                     GPYVFQANVDYENLGRFLGMYGAKVKDIKTVATIAPADEFGKEFTDAFCRAIDEFGGQ
                     VISQQWYQGEPTDLQYQINNLKMTAIEHATNHFEARFNLAKKQLVELVSTNPLYFNDS
                     LRWVVGDQYCFIFSPDTTYRLTIQDALIRTGLMKSTDFETPDPDTITFPPGIVDGLLI
                     PARANDARLIVPQLIYYDIRTPIFGSANWNEPELLRKNQAVLQNLYFISDYYIETESD
                     NYRRMAVDFKKNFGKEPERMELYGYDTMNALLTVIKSGAHTRESVRQGLLAMPVYHGL
                     CRNISFQGNRPRINSCAFLLGFKQNKIQPVAIIENGAIIEEKTKP"
     gene            complement(5696..7237)
                     /gene="guaA"
                     /locus_tag="PKX09_07285"
     CDS             complement(5696..7237)
                     /gene="guaA"
                     /locus_tag="PKX09_07285"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOU17661.1"
                     /translation="MSNISKVWIVDFGSQYTQLIARRVRDMSVYSEIVPSSVTALEAA
                     DQQVSALILSGGPASVNAVEAPQISPDFFEMDIPILGICYGLQLIARHFGARVHSDIH
                     REYGSSRIDIKKQHALFEGISTPTQVWMSHGDHVDSIPDDFEIIATSNNSVPAAMAHT
                     TRRIMGLQFHPEVEHTTEGKKILANFLFDIAGLRPDWTPEKFIEQTIAAIREQTGTEQ
                     VLMAMSGGVDSSTMGVLINRAIGRRCIPVFINHGLLRQTEQIQVVRQLKNQMGLPIRS
                     YNYSHQFLSALKGVTDPERKRKIIGREFIRAFEEIAQQFPNVKFLAQGTLYPDVIESR
                     SVNGPSQVIKSHHNVGGLPKRMNFQLIEPFSCLFKDEVRKIGRELGIPPEILGRHPFP
                     GPGLAVRILGEVTPRRLSILRRADAIYIEELRSSGYYDKVWQAFAILIPVKTVGVMGD
                     KRTYENALALRAVTSSDGMTADWARLPDTLLSRIANRIVNEVRGINRVVYDVTSKPPA
                     TIEWE"
     gene            complement(7234..8016)
                     /gene="surE"
                     /locus_tag="PKX09_07290"
     CDS             complement(7234..8016)
                     /gene="surE"
                     /locus_tag="PKX09_07290"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOU17662.1"
                     /translation="MSQDKPYILVTNDDGIAAPGINALALALKSIGEVIVIAPDSEKS
                     AVGHAITLSDPIRVTDFSKNGLWRGYSVSGTPADCIKIAVGALLERRPDLVVSGINLG
                     SNAGINVIYSGTVSAATEGTILGIPSIAISLATYSDPIFEPAAEFAIQISQLVLKKGL
                     PERTLLNINVPNVPKEQIRGIRITRQGMATFAELFDRRVDPRNRIYYWMDGKKAELEE
                     GEDVDDVAIRENLISITPVKYDLTNYEFIPELQNWLKEREEK"
     gene            complement(8003..8848)
                     /gene="accD"
                     /locus_tag="PKX09_07295"
     CDS             complement(8003..8848)
                     /gene="accD"
                     /locus_tag="PKX09_07295"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOU17663.1"
                     /translation="MADWFQRTKKGLLGTEKKVIPDGLWVKCPTCNEYLYKRELDKNY
                     WICTHCSYHFRIRCEEYIKMLVGDKFEEFGLELQSDDPLKFKDSKKYTERLKQAMSQT
                     GSNEAVRTVTGQVDDVPVVLCVMDFNFIGGSMGSVVGEKIARAIDKAREMHLPLIIVS
                     QSGGARMQEGIFSLMQMAKTSAKLTRFHEDGGLFISILTDPTAGGTTASFAMLGDLII
                     SEPGAFIGFAGPRVIKQTIGQELPEGFQHAEFLLEKGFIDIISPRSELHQLVSKILKF
                     FYEPR"
     gene            complement(8859..9308)
                     /gene="rimI"
                     /locus_tag="PKX09_07300"
     CDS             complement(8859..9308)
                     /gene="rimI"
                     /locus_tag="PKX09_07300"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOU17664.1"
                     /translation="MAAKIEARIRRANESDLTAILAIEEKSFTSAWTEDFFRHELYNP
                     VSRFYVLEKEKKIIGYIVIWLLADESHIANIAVHPEFRNAGLGGKLLNFAFEIARRNG
                     AKTITLEVNEQNIAACRLYEKFQFVLTGRRSHYYENKDDALILTRPI"
     gene            complement(9293..9955)
                     /gene="tsaB"
                     /locus_tag="PKX09_07305"
     CDS             complement(9293..9955)
                     /gene="tsaB"
                     /locus_tag="PKX09_07305"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOU17665.1"
                     /translation="MNSISPRLILGIETATLNCSVGLVHGNQIVGQKNILNRAVHSEK
                     LIELVDEVLKPDFTFADVNAIAVSIGPGSYTGLRIGLATAKGLAFHRQLPILPVPTLT
                     ALEATVRREEIGDAIFFIKSHRDLVYYTVVKKNEPLILKREIAYAPIGMVADLYPQHL
                     LIGDNAFDGQFGERLTVCFPMGEIVARLGADYFEDLLPLSHKAIEPDYHSNLEVKKWP
                     LK"
     gene            complement(9987..10457)
                     /gene="tsaE"
                     /locus_tag="PKX09_07310"
     CDS             complement(9987..10457)
                     /gene="tsaE"
                     /locus_tag="PKX09_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOU17666.1"
                     /translation="MTLQLVSHSAEDTIRLGERLATYLAPGTVVALIGELAAGKTTLI
                     KGICRGLGVTRPIESPTFTLVNEYPGSIYHIDCYREQRLMEWLELGINEYLYSDGITL
                     IEWADRIQPLLPETTLFILIRQDFNDDTVRYLEIQGSPELISQIANLQFVPNQL"
     gene            complement(10461..12029)
                     /locus_tag="PKX09_07315"
     CDS             complement(10461..12029)
                     /locus_tag="PKX09_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HOU17667.1"
                     /translation="MSPKKGKILWVDDEIELLKAHILFLRSRGFEVVPASNGIDAINL
                     VSQENFDLVLLDEMMSGKDGLETLSDIKARKPGLPVIMITKNEEESLMEKAIGSKITD
                     YLTKPVNPSQVLMACKKILEQQRIASEHVSREYLEEFHVISQRLFNDLATNDWIEIYQ
                     KLARWEIEFDDHPDLGLNETLEDQKRDCNIEFAKFITKNYKKWVNDDPHFRPILSPDL
                     IPQKVLPLLQNGQKVLFLVIDCMRLDQWYTFAPLLYDFYNIDVDYYFSILPSATPFSR
                     NAIFSGLFPSEIQLLYPDIWMPDDEDETSMNRHEAVMLEKLLSEAEIDLRGKFNYMKI
                     LDSDSGWALEKRIASYLGNSLIALVLNFVDILAHRRVDSDFLKEILPNESSYRSVVRT
                     WFQHSWIYSVLRKFADSDFTIILTSDHGSIRVQNDVKVIADKTTSTNIRFKSGKNLNC
                     PPKYALNIKNPTEYKLPDLGINTNYLIAKENFYFVYPTNYHKFQTFFKDTFQHGGISM
                     EEMILPIVTMTPKK"
     gene            complement(12219..14477)
                     /locus_tag="PKX09_07320"
     CDS             complement(12219..14477)
                     /locus_tag="PKX09_07320"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU17668.1"
                     /translation="MKINTLKQAIGGIIIALVLALLVSIFLVFGIFREMELKSLDARF
                     KNRGPKDISHSKLIIVAIDDQTFKSCRERYPYPREYYATLIENLEEVGIKTIMFDIQF
                     TEPDYKNPLGDSILADAIRKNGNVILAGKVTREFSVGGVNVYADKPLPILLETGSDWG
                     VVGEIQDPDGFTRRYPIFEQYQDKTIYTLGTKVFFKETGLPRAPEYKFENGYFIFTDS
                     TTGNTQRLLAHRGSLGWEQTILINYYGPAGTFPTYSMSAIIDDSDFDLKDEEDTDYLE
                     MFKRNSTYPLEMRIALIADSTRQFEAIAALAGGDTASVLGILDEINPFKGKIALVGVS
                     IEELHDNKFTPFYNYGADRRLMPGVETHAHAIQTMLDQSFIRSLPITLNIILIFVISI
                     LTAVAVAFARPVLGGVLALILGFGALVLSYWTFRAHAVWVQLVPLLVATGTSYLSSVV
                     YQFLFEQKEKRKIRSMFQTYMSPKILKYLEDHPDAFSLSGEKREATMFFSDVANFTTI
                     SESLSAEELALVLNKYLSPMTEILMKYDGYVDKYEGDAIMCDFGVPMEDTDHAWKACF
                     AAIDQQAALEVLRPQIKKDHGVDIFVRMGINSGVVSAGNMGSNQRFQYTVMGDAVNQA
                     SRFEGANKQYGTYMMIGEETYRRAQDKIEARILDRLVVKGKLKPITVYELLGRKGQVE
                     PKMLQICAYFTEGINLYWNRQWDAAIAAFEKALAVKGEDKPSRVFIERCHIYQENPPG
                     PDWQGEFVMETK"
     gene            complement(14493..17105)
                     /locus_tag="PKX09_07325"
     CDS             complement(14493..17105)
                     /locus_tag="PKX09_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17669.1"
                     /translation="MIELKNKSVRRIVINLSLILTLFSGLAYSQSKILHLPPREVYTD
                     SPVLIEAIIDQHTTEIREVKIYYRESGQEAFIEDYMTEIMGVFKYNIPAQFVTANGIE
                     YLIIAEFVDGSMAAFPEVDPFNVPMFVNARRMTSEPPIALDAVQTDLTGGNSSDVIIL
                     SPEEAQVVAASEVLIAVSLFNAKEIALHTVKIEVDGTNITPIAQIEEDLVLARPNNLP
                     PGNHTVTITLADPNGIYYDPLQWNFRVVGSVREAERVFNYNGRVLAEVNSEQVRGISQ
                     NIQQVTSGFNGSYDWIKFQANVFVTSEESPHKQPRNRYTAGFSSKFFEMNFGDINPQF
                     SEFGLNGKRVRGVEAHLKLNFFNLHVINGETERSIAGIISDTPQMLVSSGDTLYEYSR
                     TGYTYAQRLLAVRPYFGSGKHFQLGFNFIKAIDDTLSVHKEMNGIFAGGNQIIRMEGN
                     TPQDNIVIGSDLKLAFDNQRFVWKNAVALSMLNRNISGGALSKDDTLALGDFNIPIST
                     FGFDPSSIEKMFIINKYLYPISPIALDSVENVQNPIFKIQVDDSTQLAFGINEILNMP
                     SAAFRSTLNLNYFHNYITIKYQHIGSSFYSLGNPYMRKDIQGYLISDRIRLFQNKIFL
                     NINYEQNRDNLNNDKNATTTTSSFLAGISLFLGENLPRIDLNTVQYGRKNDVTNIDTT
                     WYGIPANSFSIKDNRESNITTRQDIRISHNINFLQIKNTLNFSWSSSDRSDRLAGSRP
                     EGYLFSSMKTSMITFSINSNFPFPLQTNLSISNNHSESGLNPEPYDFISIGAQGQYDL
                     FNKALGIYTGYGLTSGKGLADYTQNNIFGGFVFNFRKIHQLRSNVRYVYLDDRSSGEK
                     FHDLCYFVIYSLNF"
     gene            complement(17133..17822)
                     /locus_tag="PKX09_07330"
     CDS             complement(17133..17822)
                     /locus_tag="PKX09_07330"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOU17670.1"
                     /translation="MKKNILMKLLLALILFVTIGFSQDKIALILKVRGETSLKRATDS
                     EFKKGVKIGDNLYSRDRIKTGKDGFTALVFLDDKSQIKVRENSEMEITGERSKGSISK
                     QVDMQFGTLKAEINPTKKGDFIIATPTSVASVKGTVFWVLSDPINGDVFYGISGSIEV
                     TNNESGGVIVVGANQTGISTPTGQVKVEDTPAGAQPVDEDAEESEENNELRIQLRNPS
                     GETKTIKIEYK"
     gene            complement(17834..19057)
                     /locus_tag="PKX09_07335"
     CDS             complement(17834..19057)
                     /locus_tag="PKX09_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17671.1"
                     /translation="MKIISKILLFIILLISIVSAQDVNGVLDLRTNYFYNFEVYYLLD
                     FDFNNGRNNPDMFSYSLSYTPADGITPIQIKIEFEWIANIPAVNMYNHRILYVVTRPF
                     EFNGEVTLTTRDIDQNMHNIYYNDGRPCNISVDHFEQDLQRLQSIILSTGGKLPAGSY
                     VFTLNITDQYNTPLKSQTQVIEVSNPTTLELISPGGELADNFEIATTLPTFQWESIDF
                     MWSKTNCPQCGYAIRVAEYDPTRHNSVDQALKDRANLPFPDNNDYFPLPSSKISTVAG
                     LDLYTAGTIFQYPLTGAKPLEAGKIYVWQIRKTFPTTSGPESILSPIFAFKIQSMGQT
                     SYTGIDVSQYLVLINQIPEINPEWFKEGGLLYGYVPTGTVILNGNQSITLEQLSVLVG
                     QLMTGQVSIKSITIE"
     gene            complement(19242..19358)
                     /gene="rrf"
                     /locus_tag="PKX09_07340"
     rRNA            complement(19242..19358)
                     /gene="rrf"
                     /locus_tag="PKX09_07340"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(19422..>19805)
                     /locus_tag="PKX09_07345"
     rRNA            complement(19422..>19805)
                     /locus_tag="PKX09_07345"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         5636 a         4443 c         4131 g         5595 t
ORIGIN      
        1 acgcacgccg ggcgtagttc ttaatacttc ttgagtcaat ttctgaagag cgccagtgcc
       61 tttgccatga ataatttcta cctggttcaa tccagccagc aaggcgcgat caagaaattt
      121 ctggagcgca ctttcggctt cgtcgtagcg cattccccgt aaatccagac gtggtgaaaa
      181 aagcggttca ctctctactg aaactattgc ggcttgcttg cgcgaagcag tgcttttatt
      241 gaaaggatgc aggactgtcc gactaaccca gagaatttta ccgcctacgg cgactccaaa
      301 acggctattc tgcgaaccga ttttgattat ctcgcccggc tcgctaatac cgtctatccg
      361 taccgtgtca ccaatttgga attccacctg cgctaccgga gcggcgcgct ttgaacgctc
      421 cttgattttg cgaacgttcg tctggatttc ggtaatagta ctttgcgcct tatgaatagt
      481 ctcgggcgtg gcggcttgtt cgcgaatctc tttgactaca ttttcgatcc ggcgacgcga
      541 ttcatttaca agttcttcta attggcgtgc cagttcggta tcagcccgtt cggctttttc
      601 acgcaaggta ttgagtttat tattgtattc ggttactaat ttatcgagcg tttttttatt
      661 ttgggagatg atctttttat ccttttcgac ttctgaacgc aattgttcaa cttcgcgtaa
      721 aagattagtc aaacgcaccg attcttcacc gacgatctgg cgcgcattat caaccacttt
      781 cgtcggcaga ccaagccgat tggaaatttc aaaggcataa ctgctacccg gcaaacctat
      841 ttgcagacga taagtcggcg ccaatgttac catatcaaac tccatagcgg cgttaatggc
      901 gccgggttgt ttttgggcaa attctttcaa ggcgttatgg tgggtcgtaa caattatcag
      961 cgagccggcg gcaatcaatt cggttaaaat cgcttgcccg agagccgcgc cttcagtcgg
     1021 ttcagtaccg gtaccgagct catcaatcag gactaaagtt cggtcatcgg cagtttcaag
     1081 aaaatatttc atattgttaa tatgcgagga aaaagtcgaa agatcatttt cgagcgattg
     1141 ttggtcgcca atatcagcca aaaactgagt aattagcgga aggcggcttt cttcttcggc
     1201 tgggattggt aatccactca ttgccatttg tgataggaga ccaacggttt tcatcgcgac
     1261 ggtttttccg ccggcatttg ggccagagat gacaacggcc cggaaatcat tcgccaattc
     1321 aaaatccagc ggaaccactt ccttatggag cgctaaaaga ggatgccgcg cttgatgcaa
     1381 aacaaaatta ttattctcgg caataatcgg cggtgtacat ttgaattttt gcccaaaacg
     1441 cgcgcaggca ttatacaagt cgagttcagc cagaatttgg atattttttt gtaaaggcga
     1501 gaagtccggc cgcattttat cggttgtttg gcgtagaata cgctcgattt cttccttctc
     1561 agccagttcc agctctttaa gagcattatt aatttctacg atctcgagcg gttcaacata
     1621 aacggtggca ccggtggctg attggccgtg aattactccg ttaatttttc gtttcgattc
     1681 aactcgtaga ggcaaaacca aacgtccgtc gcggatagtt gcctgatctt catgaagcca
     1741 ttgctgatcc cgcgcgtagt tcatcaaacg ctcaatctca tgattgaggc gacgctggtg
     1801 agtgcgaatt tcatggcgaa ttcgattaag ctgcggactg gcgctatcgc gaagcgagcc
     1861 atccggcgcg ataatagccg cgatagtttt acgtacttgt ggcagagaat ccaaaccttc
     1921 aaatagttct tccaaactca atagttgcgg acgctgagag cgaaaaaaac gacgttggcg
     1981 ctccaccaat tccagtaatt gatcaatttg aatcagagtt tcaagagata aaaatgttcc
     2041 gatgacacga catcggtcaa gttcggcgcg gaaatcggaa aaagcattca acggtagcga
     2101 atcaccagcg gatagaatgg cttggagcgc attgatgcga ctttgccggc atcgaatcat
     2161 ctcaatatcc tttagtggca aaatagtatt agccagagat ttgccggcag tagagagagc
     2221 catttcctga atcaactcga tgattttagc aaaaccgagt ttatcgtttg tttccttgat
     2281 cgagtcgggc attgttacta attcagggat tatttttact tggagaaata ttcgtatctt
     2341 ttggatttgt ctctgaggtt tccggcaaga aaacgatttt acgcatactt ttacggagat
     2401 gatccatctc cgaattcagt ccgcaaattt tgataacttt ctcgccactg cgttcaaaaa
     2461 acttaaaatt ggctgaagat tgacgcagat ggtccacgaa tccgagttct ggatagacat
     2521 ttaataccca gactattccg atcataataa tggcaccttc caggaatcca aacagcattc
     2581 cgccaattcg gtcaacccat atcaatttca aagctcgtaa aaggcttctc aacattgaag
     2641 ccagcaattc aaaaagtaaa aacacaacga tgaacacgat aacaaaacca atcaccgact
     2701 gtaccccttc ggatagatta aaaaggtcgg caattatttt cccaccaaaa taataatatc
     2761 gtgaagcaga gagcgccgcc aatactaagc caataagctt aaaagccgat tttatcaagc
     2821 cattgcgtaa tccgaaaaaa atgcaaactc caataattac ggctactatt aaattaaaca
     2881 tccgaatcta tcacttagca tttatttcgc tgattaatgc caattacagc cagcatccgt
     2941 ccggtaacgc cctcatcccg ccggtaggaa taaaattttt caggttgaca agcggtacag
     3001 taaccactat cggcaatcct atcgagttga acaccagccg cgttcaagcg attggttatt
     3061 gccaggatta aatcgagaaa aagaccctct tcccgctgga ttatttcatt atcgctaaac
     3121 tttttggcga cattattttt cactttataa caacagacat gcaaagccgg accgatggcg
     3181 gctattaatt cgcccggatt agtttggaac tgagcttgca tagtattaat ggtgttggcg
     3241 attattccct cggctgtgcc ccgccaaccg gcatgcactg ccgcaatgac ctggtggtgt
     3301 ggatcataca ataaaacagc cgcacaatca gccgttttaa tactcagata taaattggct
     3361 tgagtggtaa ttagagcatc gcaacctgga ataggaccat tgtcttccac tatagcgatc
     3421 ttgttgctat gaacttgttc ggcttgagca acatcggacg aagtaaaatt gagggtatta
     3481 taaaatcttt ttcgattaat caggatattc ttaagagtat cgccgtcggc gattccaaga
     3541 ttaagtgaac ggaaagggcc attacttact ccaccgttcc gggtgctgaa agcgtggaca
     3601 atttccggaa aagtcattaa ttccggagat tggatgattt caattccttt ggaaacggtt
     3661 agtggaaaca ttatggctta gttttttctt caataatggc accattttcg atgatcgcca
     3721 ctggttggat tttattttgc ttgaaaccaa gtaggaaggc acaggaattg atgcgagggc
     3781 gattaccctg gaaggaaata ttacgacata gtccgtgata gaccggcatt gctaaaagcc
     3841 cttgccgcac cgattcccgc gtatgcgcgc cgctcttgat tactgtgagt aaggcattca
     3901 tcgtatcata cccgtataat tccattcgtt ccggttcttt gccaaaattt ttcttaaaat
     3961 caaccgccat ccgacgataa ttatctgatt ctgtctcaat ataataatcc gaaataaaat
     4021 acaaattttg cagaactgcc tgattcttcc ttagtaattc cggctcattc cagttggcac
     4081 taccaaaaat aggagtgcga atatcataat aaattagttg gggaacaata agccgggcgt
     4141 cgtttgcgcg agccggaatc agtaatccat ccacaatacc tgggggaaaa gtaattgtat
     4201 cgggatcggg agtctcaaaa tccgtactct tcatcaagcc ggtccttata agagcatcct
     4261 ggatcgttag acgataggtg gtatcgggac taaaaataaa acaatattga tcgccgacaa
     4321 cccaacggag tgaatcatta aaataaagag gattggtgct tactaattca actaattgct
     4381 tctttgccag attgaagcga gcctcgaaat gattagtagc atgctcaatc gccgtcatct
     4441 tcaaattgtt gatctggtac tgaaggtcgg tcggctcgcc ttgataccat tgctgagaaa
     4501 ttacctgtcc cccgaactca tcaattgcac ggcaaaaagc gtcggtgaac tccttgccaa
     4561 attcatccgc cggtgcgata gtcgcaacag tttttatatc ctttactttt gctccataca
     4621 ttcccagaaa acgtccgaga ttttcataat ccacatttgc ctggaaaaca tagggtccca
     4681 gtgatgccag ttgactacca gaagccgtcg gcgtgatcat cggaatttga tactgattgg
     4741 caataaccgc aatcaaaacg gcattttcag tcattagcgg gccgaagata gctagtactt
     4801 tgggactcct agctaaaaat tcggcttgct tgatactcag aacgccatcg ctccgattat
     4861 caaatacgag ggcaacaata tcctcggaat tattcttgcg ataattagca atggcgtatt
     4921 tgacaccatt caaaatttct cgaccggaag ctgaatttga gcccgacaat ggcaggatta
     4981 ctccaatgta tttccgaacg acggacggct tcttcagctg aacttcgact ttctgtaatt
     5041 ggtcgcgcaa atatttattt tcgagacgag caggcaatcg ctttagctcg gcttcagcta
     5101 agtcagtgtg tccttcggag tgatatttct cggctaattt tatcgttaaa tattgcctcg
     5161 aaaaattatc ggaagtcgag tcacgcaggg cgttgactgt ctcagcctcg agataaaaat
     5221 caattatctt attgaggtag tcttcacatt gcgactggat ctcctgatcg gtataatttt
     5281 tcaataaatc cagataaatt cgcagtgatt cctgataatt ccccagcgat aattgcactt
     5341 ctgctaaagt cagttttact tccttaacat atttcgattc cggataattc tggagaaaat
     5401 cgtaagccaa agcgatactc ttctctgctt cgcccaggcg gtaagtcgtt ttaatcagca
     5461 ttaaagcgga agttgtcaag tagggatttt cgtctttggg tatggaatta atgattttaa
     5521 aaatattctg agcatcccag tatttttcgg cgttataaca ggctattccc tcatcgagca
     5581 aacttcgcag tttgtcagtc gtttggtcgg ctaacaagct cgacccccaa attagggaaa
     5641 taacgagaat aaaggtagcg gttctgggta atttcatcat catctaaaac ttaatttatt
     5701 cccactcaat cgtagctggt ggtttggaag tgacatcata gacgacgcga ttgatgccgc
     5761 gaacttcatt gacgattcgg ttagcaatgc gactgagtaa agtatccggt aagcgcgccc
     5821 aatcggcagt catgccgtca ctgctcgtga ccgcacgcaa agctagggca ttttcatagg
     5881 tgcgcttgtc gcccataacg ccgactgttt taaccggaat caggattgcg aaagcctgcc
     5941 agaccttatc atagtacccg ctggaacgta gttcttcgat gtaaatggca tccgctcgac
     6001 gtagaatgct aagtcggcgg ggagtgactt cgcccagaat acggacagcc aatcccggac
     6061 ctgggaatgg atgccgtcct aaaatttcgg gggggattcc gagttcacgc ccgattttgc
     6121 gaacctcatc cttgaagaga cagctaaaag gttcaattag ctggaaattc attcttttcg
     6181 gcagaccacc aacgttgtga tgacttttaa ttacctgcga gggaccattt accgagcgac
     6241 tttcgatgac atccggataa agtgttcctt gcgccagaaa ttttacattc ggaaattgct
     6301 gggcaatttc ttcaaaagcc cggataaact cccgcccgat tattttgcgt ttacgctcgg
     6361 gatcggttac tcctttcaag gcactaagaa attgatgcga ataattataa ctacgaatcg
     6421 gcaatcccat ttgatttttt aattgccgga cgacttgaat ttgttcggtc tggcgcagta
     6481 gcccgtgatt gataaaaacc ggaatacagc ggcggccaat ggctcgatta atcaggaccc
     6541 ccatcgtgga ggaatccact ccaccgctca tcgccattaa aacttgttcc gtgccggttt
     6601 gctctcggat agcggcaatc gtctgctcga taaatttttc gggagtccaa tctggacgaa
     6661 gcccggcgat gtcaaagagg aaattagcta agattttctt cccttccgta gtatgttcga
     6721 cttccggatg aaactgaagt cccataatcc gacgagtggt atgcgccatc gcggcaggta
     6781 cactattatt tgaagtcgca ataatttcaa agtcatctgg tattgaatct acgtgatcgc
     6841 catgactcat ccagacctga gtcggtgtcg aaataccttc aaatagagca tgctgttttt
     6901 tgatatcaat cctagatgaa ccgtattctc gatgaatatc agaatgaacc cgcgctccga
     6961 aatgccgcgc aatcagttgc aagccataac aaatccccaa tatcggtata tccatctcga
     7021 aaaaatcagg agaaatctgt ggcgcttcga ccgcattaac gctggcagga ccaccggata
     7081 gaattaaagc acttacttgc tggtcagcgg cttcaagggc agtcacactc gacggtacaa
     7141 tttcggaata aactgacata tcccgcaccc gccgcgcgat taattgggta tattgtgaac
     7201 caaaatctac aatccaaact ttactaatat tactcatttt tcctctctct cttttagcca
     7261 attctgcaat tccggtataa actcatagtt ggtcaaatca tacttgacgg gcgtaatcga
     7321 aatcaaattc tcacgaatag ccacgtcatc aacatcctca ccttcttcga gttcggcttt
     7381 tttaccatcc atccaatagt aaatccggtt gcggggatcc acccttcggt cgaagagttc
     7441 ggcgaaggtt gccatcccct ggcgtgtaat ccggatgccg cgaatttgct ccttcggcac
     7501 attagggaca ttaatgttca ataaagtccg ctccggtaag ccctttttta gaacgagttg
     7561 agagatttgg atcgcaaatt ctgcggctgg ttcgaaaatt gggtcagagt aagtggcgag
     7621 tgagatggca attgagggaa ttcccagaat cgtaccttcc gtagccgccg aaaccgtccc
     7681 ggaataaatt acgttgatgc cggcattgga accgagattg attccggaaa cgacgaggtc
     7741 tggtcggcgt tccaaaagcg cgccaaccgc aattttgata caatcggctg gtgtccccga
     7801 gactgaatag ccacgccata atccattttt actaaaatcc gtcacccgga ttgggtcaga
     7861 tagagtaatg gcatgaccaa ctgcactttt ttcactgtcg ggcgcaatga caattacttc
     7921 accgattgat ttcagagcga gtgccagcgc attaataccc ggcgcggcga tgccgtcgtc
     7981 attagtaacc agaatatagg gcttatcttg gctcataaaa aaacttcaaa attttactga
     8041 ctaattggtg gagttccgaa cgtggcgaga taatatcaat aaaacccttt tcaagtaaaa
     8101 attcagcatg ttggaagcct tctggcaatt cctgaccgat ggtttgctta atgactcgtg
     8161 gcccagcgaa tccgatgaaa gcacccggtt cggaaattat caaatcgccg agcatcgcaa
     8221 agctcgccgt cgtaccaccg gctgtcggat cggttaaaat agatataaat aatccaccgt
     8281 cttcgtgaaa acgcgtcaat ttcgcgctag tcttagccat ttgcattaag gaaaaaatcc
     8341 cttcttgcat ccgcgctccg ccggattgag acacgattat caatggtaga tgcatttcgc
     8401 gggctttatc aatagcgcgc gcaatttttt cacccacgac cgagcccata ctcccgccga
     8461 taaagttaaa atccataacg cataatacca ccggcacatc atcaacctga ccggttacgg
     8521 ttcgaaccgc ttcgttcgag ccggtctgac tcattgcctg tttcaaacgc tcggtatatt
     8581 ttttagaatc tttgaatttt aaaggatcat ccgactgcag ttccaatcca aattcttcga
     8641 acttatctcc gactaacatt ttgatatatt cttcacagcg aatccggaaa tggtagctac
     8701 aatgagtgca aatccaataa tttttatcga gttcacgttt ataaagatac tcattacagg
     8761 tcgggcattt tacccagagt ccatcaggga taactttttt ctcggttccg agtagacctt
     8821 tttttgttct ttgaaaccag tcagccatat ttacctcatc aaattgggcg agtcagtatc
     8881 agagcatcat ccttattttc ataatagtgc gaccgacgtc cggtcaaaac aaactgaaac
     8941 ttttcatata aacgacaagc ggcaatattt tgctcattaa cttccagggt tatcgtcttt
     9001 gctccattac gacgcgcaat ttcaaaagcg aaatttaata attttccacc taaaccggca
     9061 tttctaaatt cggggtgaac ggcaatattg gcgatgtgcg attcatcggc taacaaccag
     9121 atgacaatat agccgatgat tttcttctct ttttcgagta cataaaaacg actaacggga
     9181 ttgtataatt cgtgccggaa aaaatcttcc gtccaggcag atgtgaacga tttttcttcg
     9241 attgccaata tagccgttaa atccgactcg ttagcccgac gaattcgggc ctctatttta
     9301 gcggccattt cttgacctcc aagttggaat gataatccgg ctctatagcc ttatgactta
     9361 acggcaataa atcttcaaaa taatctgcac ccagtcgggc aacgatttcc cccatcggaa
     9421 aacataccgt cagtcgttca ccaaattgcc cgtcaaatgc gttatcgcca atcagcaaat
     9481 gttggggata aagatcggca accataccga tcggagcgta ggctatttct cgtttcagaa
     9541 ttaacggctc atttttttta actacagtat aatataccaa gtcgcgatgg gactttataa
     9601 aaaagatcgc atctccaatt tcttcccgcc gcacggtagc ttccaaagcg gttaaagtcg
     9661 gcaccggtaa aatcggcagt tgccgatgaa acgctaaccc cttagccgtc gccagaccaa
     9721 ttctcaatcc ggtatacgag cccggtccga tgctaactgc gatggcattt acatccgcaa
     9781 aggtaaaatc aggctttagt acctcatcaa ccaactcaat tagcttctca gaatggactg
     9841 cgcggttaag aatatttttt tgacctacga tttggttgcc atgaacgaga ccaacgctac
     9901 aattgagcgt tgccgtttca ataccgagga tcagtctggg agaaatagag ttcaaagttc
     9961 aaagttcaaa gttttaaagt aggaattcat aattgattag gaacgaattg aagattggca
    10021 atttgggaga tcagctcagg cgaaccctgg atttccagat agcgaaccgt gtcatcgttg
    10081 aaatcctgtc gaatgagaat aaataaagta gtctccggca gaagcggttg gattcggtca
    10141 gcccattcga tcaaggttat accgtcgcta tacaaatatt cattgatgcc gagttccagc
    10201 cattccatca aacgctgttc gcgataacaa tcaatatggt aaattgagcc ggggtattca
    10261 ttgaccaagg taaaagtcgg actctcgatt ggtcgggtta ctcccaaacc acgacagata
    10321 cctttgatta gcgtggtctt accagcggct aattcaccaa tcaaggcgac caccgttccc
    10381 ggagctaaat aggttgccaa tctttcgccc aaccggatag tgtcctcggc actgtgacta
    10441 actaattgca aagtcatagt ctatttcttc ggcgtcatag taacaatcgg taatatcatt
    10501 tcttccatcg agatgccgcc atgttggaag gtgtccttaa aaaaggtttg aaatttgtga
    10561 taattagtcg gatagacaaa atagaaattt tccttagcga tgaggtaatt ggtattaata
    10621 cccagatctg gtaatttata ttcggtggga tttttgatat tcagggcata tttcggtgga
    10681 caattcaagt tcttgccgct tttaaaacga atattggtag aagtagtttt atcggcaatt
    10741 accttaacat cgttttgaac gcgaatactg ccatgatcgc tggttaaaat aatggtaaaa
    10801 tcggagtcag caaatttccg taagaccgaa taaatccagg aatgctgaaa ccaggtacgc
    10861 actacggaac ggtagctgga ttcgttcggc aaaatttcct ttaaaaaatc ggaatctacc
    10921 cgacgatgtg ccagaatgtc aacaaaatta agcaccagcg cgatgagtga attacctaga
    10981 taagaggcaa tgcgtttctc gagagcccag ccactgtccg aatcgagaat tttcatataa
    11041 ttaaatttcc ctcgcagatc aatttcggct tctgataaca atttttcaag catcacggct
    11101 tcgtggcgat tcatcgaagt ctcatcctca tcatcaggca tccagatatc gggataaagc
    11161 aactgaattt cactgggaaa aagcccgcta aaaatagcat tgcgagaaaa aggggtagcc
    11221 gaaggtagaa tggaaaagta ataatccaca tcaatattat aaaaatcata caataacggc
    11281 gcaaaagtgt accactgatc caaccgcata caatcaataa caagaaacag taccttctgt
    11341 ccattttgca aaagaggcaa cactttttgc ggaatcaaat ccggcgaaag aatcggacgg
    11401 aaatggggat cgtcattaac ccatttcttg taatttttag tgatgaattt ggcaaactca
    11461 atattacagt cgcgtttttg atcctcgaga gtttcattca agcctaaatc aggatgatcg
    11521 tcgaattcga tttcccatct ggctaatttc tgataaattt cgatccaatc attggttgcc
    11581 aggtcattaa ataatcgttg agaaataaca tgaaattctt caagatattc gcgggaaacg
    11641 tgttcggaag caatccgttg ttgctcgagt atttttttac aagccattag cacctggctg
    11701 ggattgactg gcttggtaag gtagtcggta atcttactgc cgattgcttt ttccatcagg
    11761 ctctcttctt cattcttggt aatcataatt accggcagac cgggtttgcg ggctttgatg
    11821 tcggatagag tctccagtcc atctttacct gacatcattt cgtccagtag aaccaaatcg
    11881 aaattttcct ggctaactag attgatagca tcaatgccat tcgacgccgg aactacctca
    11941 aaaccgcgac tacgcaagaa tagaatatgg gctttcaaca attcaatttc atcatcaacc
    12001 cataatattt ttcccttttt aggactcata gaattactca atcactcttt ttattgttaa
    12061 taattccaag gaacgatttt gtttcaggat tcataatttt tagaacttaa aattaaacaa
    12121 accaccagct aatttacgca aaatggtctg tggtaaaaga taaatttata ggaaaataaa
    12181 tgtttaaatc ggttagacga tccgaagaac agctgccgtt atttggtttc cataacgaac
    12241 tcgccttgcc aatccggccc aggtggattt tcttgataaa tatgacagcg ctcaataaaa
    12301 acgcgcgacg gtttgtcttc tcctttaacg gcaagagctt tttcaaatgc ggcaatggcg
    12361 gcgtcccact ggcgattcca atagagattg attccttcag taaaataggc gcaaatttgc
    12421 aacattttcg gttcaacctg acctttgcga cccagaagtt catataccgt aattggcttc
    12481 aatttacctt ttaccacgag gcggtcgaga atgcgagctt caatcttgtc ctgcgcccgg
    12541 cgataggttt cttcaccgat catcatatac gttccatact gtttattagc accttcaaaa
    12601 cgagaagcct gattgaccgc gtcgcccata actgtgtact gaaagcgctg attggaaccc
    12661 atattaccgg cactgacaac tccggaatta atccccatcc gtacaaaaat atccacgccg
    12721 tggtctttct taatttgagg gcggagaact tccagcgccg cttgttgatc aatagccgca
    12781 aaacaggctt tccaagcatg atcggtgtct tccatcggaa caccgaaatc acacataatc
    12841 gcatcacctt catatttatc cacgtaaccg tcgtatttca tcagaatttc tgtcatcgga
    12901 gataaatact tatttagaac taaagccagt tcttcggcag agaggctttc agatatggta
    12961 gtaaaattag cgacatccga aaagaacatc gtggcttcac gcttttcacc ggaaaggcta
    13021 aaagcgtcgg gatgatcttc aagatatttc aggattttcg ggctcatata agtctgaaac
    13081 atactccgaa ttttcctttt ctctttttgt tcgaagagaa attgataaac gacactcgat
    13141 aaataactgg taccggtagc taccaagagc ggcaccaatt gcacccagac agcgtgggca
    13201 cgaaacgtcc agtatgataa tacgagcgca ccgaaaccca atattaatgc taaaacccca
    13261 cccaataccg gtctggcaaa agccactgca acggcggtga gtatgctaat gacaaagatt
    13321 aagatgatat tcagtgtaat cggaagggaa cgaataaaac tctgatccag catagtctga
    13381 atagcatggg catgcgtctc gactcccggc atcagacgcc gatcagcacc gtaattatag
    13441 aacggtgtaa atttattgtc atgcaattcc tcgatagata ctcccaccaa agcgattttt
    13501 cctttaaacg gattgatttc gtcaaggatt cccaaaacgc tggcggtatc accgcccgcc
    13561 agagccgcga tagcctcaaa ttgacgggtc gaatccgcaa tcaatgcaat gcgcatttcc
    13621 agtgggtagg tcgaattcct cttaaacatc tcaaggtaat cggtatcttc ctcatctttc
    13681 aaatcaaaat cggaatcatc tattatggcc gacatagaat aggttggaaa ggtgccggct
    13741 ggcccataat aattgatcag gatagtttgt tcccaaccaa gactgccgcg atgcgccaga
    13801 agacgctggg tattaccggt cgttgaatcg gtaaaaatga aatagccatt ttcaaattta
    13861 tattccggtg ctcgaggcag acccgtttct ttaaaaaaaa ctttggtacc gagagtatag
    13921 attgttttat cctgatattg ttcgaaaatc gggtaacggc gggtaaatcc atcaggatcc
    13981 tgaatttcgc cgacaactcc ccagtcacta ccggtttcca gcagtatcgg caaaggctta
    14041 tcggcataaa cattgacgcc gccgacgcta aattcccgcg tgacttttcc agccagaatg
    14101 acattaccgt ttttccgaat agcatctgcc agaatcgaat cacccaacgg atttttataa
    14161 tccggctcag taaattgaat atcaaacata atcgttttaa taccgacttc ctccagattt
    14221 tcaatgaggg tagcataata ctcgcgcgga taagggtagc gttcacggca gcttttgaaa
    14281 gtctgatcgt caatggctac aatgattaat ttggagtgtg aaatgtcctt cgggccgcga
    14341 tttttaaacc gcgcgtccag cgatttcaat tccatttcac gaaatatacc aaacactaaa
    14401 aaaatcgaaa ccagtaacgc cagaacgagg gctataatta ttcctcctat agcctgtttg
    14461 agcgtattta ttttcattag aggcttaagt atctaaaaat tcagggaata gatcacaaaa
    14521 tagcaaaggt catggaattt ttcgccactg cttcggtcgt caaggtaaac gtatctgaca
    14581 ttactacgca gttggtgtat tttcctgaaa ttaaagacaa atccgccgaa aatgttattt
    14641 tgggtgtagt ctgccaatcc cttaccactt gtcaaaccat agccagtata aattcccaga
    14701 gctttattaa aaagatcata ttgcccttgg gctccgatag aaatgaaatc ataaggctcg
    14761 ggattcaaac cgctctcgct gtggttattg gatatgctaa gattggtttg cagaggaaat
    14821 ggaaaattac tgttgatact aaaagtaatc atagaagttt tcatcgaact gaacagatag
    14881 ccttccggtc ggctacctgc cagccgatca ctccgatcgg aactggacca agagaagttt
    14941 agggtattct taatttggag gaaattgata ttatgggaaa tgcgaatatc ctgacgagtc
    15001 gtgatatttg attcacggtt atccttaatg ctaaatgagt ttgcaggtat tccataccag
    15061 gtcgtatcaa tgttggtaac atcgtttttc cgcccatatt gcactgtatt caaatcaatt
    15121 ctgggtaagt tctcgcccag aaataatgaa atgccggcca aaaaggatga ggttgtagtt
    15181 gtggcattct tgtcgttatt caaattatcg cggttttgct cgtaattgat gtttaaaaat
    15241 attttgttct ggaagaggcg gattctatcg gagattaggt aaccctgaat atctttgcgc
    15301 atatagggat ttcccaatga ataaaaagag ctaccaatat gctggtattt aatagtgatg
    15361 taattatgga aataattaag attcaatgta cttcggaaag cggcggaagg catattaaga
    15421 atttcattta ttccaaaggc taattgggtg gaatcatcca cttgaatttt aaaaatagga
    15481 ttttgcacat tttccacaga atccagagct atcggtgaaa ttggatatag atacttatta
    15541 atgataaaca ttttttcgat tgagctggga tcgaaaccga aagttgagat cgggatatta
    15601 aaatcaccga gcgccagcgt atcgtcttta cttaaagcac cgccactgat attacgattt
    15661 aacatcgaga gcgcgaccgc atttttccat acaaatcttt gattatcaaa tgccagcttc
    15721 aagtcagacc ctatgacgat gttatcctga ggtgtgttgc cttccatcct tataatctga
    15781 ttaccacccg caaaaatgcc attcatctct ttatgaaccg aaagagtatc gtcaatggct
    15841 ttgataaaat taaaacctaa ctgaaaatgt ttaccggaac cgaagtaggg tcgcactgcc
    15901 agtagacgct gagcgtaagt ataaccagta cgcgagtatt catacaaagt atcaccactt
    15961 gagacgagca tttggggtgt atcgctgatg attcctgcga tactacgctc ggtttctcca
    16021 ttaataacat gtaagttgaa gaagttgagt ttcaaatggg cttcgactcc ccgcactctt
    16081 tttccattta aaccgaattc agaaaattgg gggttgatat ctccgaagtt catttcaaaa
    16141 aacttggagg aaaaaccggc tgtgtaacga ttacgaggtt gtttatgtgg actctcttcc
    16201 gaagttacaa agacgttggc ttgaaatttt atccaatcat aggaaccgtt aaaaccgctc
    16261 gtcacctgct gaatattctg ggaaataccc cgcacctgtt ccgaattcac ttcagccagc
    16321 acgcggccat tataattgaa taccctttcg gcttcacgta cactgccaac taccctgaaa
    16381 ttccattgca atggatcgta ataaatccca ttcggatccg ccaaggtaat tgtgactgta
    16441 tgattaccgg gtggtagatt attgggtcgg gcaagaacaa ggtcttcctc tatctgagca
    16501 attggggtga tattagttcc atccacttcg attttaacgg tatggagagc aatctccttg
    16561 gcattgaata aagatactgc tatcagaact tctgaagccg ctacaacctg agcttcttca
    16621 gggctaagga taattacatc gctggaattt ccgcccgtaa ggtcggtttg aaccgcatcc
    16681 agagcaattg gaggttcgga cgtcatgcgg cgggcattga cgaacatcgg cacgttaaaa
    16741 ggatcaactt ccggaaaagc ggccatactg ccgtctacaa attcggctat tataagatat
    16801 tcgatgccat tggcagttac gaattgcgct ggaatattat atttgaaaac ccccatgatt
    16861 tcagtcatat aatcttcaat aaaagcttct tgccccgatt ctcggtaata gatttttacc
    16921 tctcgaattt ccgtagtgtg ttgatctata atcgcttcaa ttaaaaccgg tgaatcagtg
    16981 tacacttccc ggggcgggag atgcagaatc ttggattgag aataggctaa tccactaaac
    17041 aacgttaaaa ttaacgataa attaattaca attcggcgta ccgatttatt cttaagctca
    17101 atcatgtatt taaaaactcg atccgatatt atctatttat attctatttt aattgttttg
    17161 gtctcaccag aaggattgcg caattgaatt cgtagttcat tattctcttc agattcttcg
    17221 gcatcttcat caacaggctg ggcaccggct ggggtgtctt ctaccttgac ttggcccgtc
    17281 ggagtcgaaa taccggtctg gttagcaccc acaacaatca ccccaccact ctcattattg
    17341 gtaacttcaa tactacctga aatgccataa aagacatcgc cgttgatcgg atcagatagt
    17401 acccaaaaga ctgttccttt gaccgatgcc accgaggtcg gggtagcgat gataaaatca
    17461 ccctttttcg tgggattgat ttccgctttc agagtcccga attgcatatc aacctgtttt
    17521 gaaatggaac ctttgcttcg ttcaccggtt atttccattt cggaattttc tcgaactttt
    17581 atctggctct tatcatcgag aaaaaccagt gcagtaaatc cgtcttttcc ggttttgatc
    17641 ctgtctcgtg aatagagatt atcgccgatt tttactcctt ttttaaattc ggagtcggta
    17701 gcacgtttga ggctggtttc gccgcgaact tttaaaatta gggcaatctt gtcttgactg
    17761 aatccgatcg taacaaataa tattaaagcc aacaataatt tcattaaaat gttttttttc
    17821 atgttaccag atcctattca atcgttatag attttataga aacttgaccg gtcattaatt
    17881 ggccaaccag gactgacaat tgttcgagcg taatactttg attaccattt aaaattaccg
    17941 tcccagttgg aacataacca tatagtaagc caccttcttt aaaccattct ggattaatct
    18001 caggtatttg atttatcagg actaaatatt gcgatacatc tattccggtg taactggttt
    18061 gccccattga ttgaatctta aaagcaaaaa tcgggctcaa gatcgactcg ggtccggaag
    18121 tggttggaaa tgttttccga atctgccaga cgtagatttt accggcttct aagggtttcg
    18181 ccccggtgag cggatactgg aagatcgtgc cagcggtata taaatccaat ccggcgacag
    18241 tggagatttt cgatgatggt aatggaaaat aatcattatt atcgggaaat ggcaagttgg
    18301 cgcgatcttt caaagcctga tcaacggaat tatgtctggt tggatcatat tcagccactc
    18361 taatggcata gccacattgc gggcaattag ttttactcca cataaaatca attgattccc
    18421 actgaaaagt cggtaaggta gtagcaattt cgaaattatc tgccaactct cctcccggcg
    18481 aaatcagctc cagcgttgtc ggattggaaa cctctatcac ctgagtttgg ctctttaggg
    18541 gggtattata ctgatcggta atatttaatg taaacacata acttccagcc ggcaattttc
    18601 caccagttga aagaattatt gattgcaacc tttgcaaatc ctgttcgaaa tgatctacac
    18661 ttatattaca tggtcttccg tcattgtaat aaatattgtg catattttgg tcaatatccc
    18721 gagtcgtcag agttacttct ccattgaact caaagggtcg ggtgactacg tataatatcc
    18781 gatggttata catatttact gccggaatgt tggcaatcca ttcgaattcg attttaattt
    18841 gaatcggcgt aattccatct gcaggagtat aagagagcga ataggagaac atatcaggat
    18901 tattgcggcc attattgaaa tcaaaatcaa gtagataata tacttcaaaa ttatagaaat
    18961 aattggttct aaggtccagg actccattaa catcctgagc ggatacaatt gaaatcagca
    19021 ggattataaa cagaagaatt ttagatatta ttttcataaa tcctccattt tattcatgct
    19081 tcaatctaaa gatattgaat taatgacatt gtgagataga acaagggtta gttcttcact
    19141 ttcatctaaa actacttcta attattgaga acacgcgata aatataacac taggggagac
    19201 gagatacaat aaaaatcccg ccgaagcggg attaaagatt ttcccggtga tgacctactt
    19261 ttccaccccg ttgccagggc agtatcatcg gcgctggagg gcttaacttc cgagttcgag
    19321 atgggatcgg gtgtttcccc tccgctatta tcaccggaaa aaaggatttt aattgggtta
    19381 aggaaaagag aacgattaga gaagtcaatt tcataaaaat tttggtcaag acgcacggct
    19441 tattagtatc actcggctaa aatcattact gatcgtacac ctgtgaccta tcaatcccgt
    19501 agtctacggg aagcctttag ttccgccgaa acggaaggga gatatcatct tggggaaggt
    19561 ttcacgctta tatgctttca gcgtttatcc tgaccgaaca tagctaccca gcgatgcacc
    19621 tgacggcaca actggtgcac cagaggttcg tccactctgg tcctctcgta ctaagagtag
    19681 ccccccgcaa atctcctgcg cccacagcgg ataaggaccg aactgtctca cgacgttcta
    19741 aacccagctc acgtgccact ttaatgggcg aacagcccaa cccttgggac cttctccagc
    19801 cccag
//
LOCUS       DAOSMP010000039        18939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_2925_length_18939_cov_15.441908, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000039 DAOSMP010000000
VERSION     DAOSMP010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 18939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18939
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2925_length_18939_cov_15.441908"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PKX09_07350"
     CDS             <1..285
                     /locus_tag="PKX09_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17672.1"
                     /translation="LVKNVRFDENYLGGMPSLEKFRIAGTEFYNEIRNMSPLKSIYNA
                     AEKKHFDVYDLGVRGSLSFSGLLKRLHSGQLLDYNLWIIIGMLILLWVAW"
     gene            318..869
                     /locus_tag="PKX09_07355"
     CDS             318..869
                     /locus_tag="PKX09_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HOU17673.1"
                     /translation="MPTETWLIFILGFMLVGSIAALEIKDLLSAIIAVGIVGLGLSMA
                     FLLLQAPDLAIVQFLFEIFAIVIMVRAFMKPGYHFSDTKSKHRGRTIIVMAVLVAIFI
                     LSINVFKALPPFGEPLMTTSQHYLENAARETGSANIVASIVLDYRGYDTLGEATVLFV
                     AVLGTLTILRVKSNKKKEEESEK"
     gene            866..1315
                     /locus_tag="PKX09_07360"
     CDS             866..1315
                     /locus_tag="PKX09_07360"
                     /inference="COORDINATES: protein motif:HMM:NF006248.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HOU17674.1"
                     /translation="MKQEPGLTLIAKTVTRVSVWLIILYGIYIILHGHLSPGGGFAGG
                     VVIALAFLNVMLTYGRETVTHWVNVEFLHDLEPACGAIYMAIGILGISISGGFLANFL
                     SKGTLFNLLSSGNILLLNIIIGIKVAMGLFTVVWTLAIFHINEGGEK"
     gene            1312..1656
                     /locus_tag="PKX09_07365"
     CDS             1312..1656
                     /locus_tag="PKX09_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOU17675.1"
                     /translation="MIVYLLCFALLLVGIYGLLVKRDLVRLIIASGITGYATNLMLVL
                     FGYKRGAIYPILEKGKAAGERIMVDPLPQALVLTSIVIELGITAMLVALAIRLFEKNK
                     TFDITKIKRLSG"
     gene            1653..3125
                     /locus_tag="PKX09_07370"
     CDS             1653..3125
                     /locus_tag="PKX09_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOU17676.1"
                     /translation="MILPFFIIIPLLTAFIITLIAGERDNWAVILSILAVVSLVILSV
                     YCFVSLNGRTLVYEMGRWQAPLGIILAVDALSAFMLVIVSIIALTAIMFSVQYIRNLS
                     PSWKYYALFMLLTGGMNGVIATGDLFNLFVFVEITLFAAYGLVAYGNRAEEFEASFKY
                     AIMGSISSTLVLVGIGIIYSATSTLTMALIAERLPQNPTVMYWSGGLFVAAFGLKAAI
                     MPFHAWLPDAHSSAPAPISAMLSGVLIKALGVYVLIRLFFNVFGEMPVFSEVFLVLGT
                     VSMVIGVFLALGQWDVKRLLAYHSISQIGYIIIGVGLGTTLGLLGAVFHLLNHAIFKA
                     LLFLNAGSLEMALGTRDLRQMGKVSKALPVTSFTSMVASLSISGIPPFNGFFSKLIIF
                     IAAIQIGRPVVAFLAGLGSLLTLASFMKVQRYGFHGEKTVTADQAKVGWGMRAAMIVL
                     AILCLATSALVIPGVREVTLDPVVEVIMNKTGYITRVLGR"
     gene            3128..3634
                     /locus_tag="PKX09_07375"
     CDS             3128..3634
                     /locus_tag="PKX09_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HOU17677.1"
                     /translation="MTIKNRSRVIVFVLSFLVWLALTDIKDIQEVVAGLIVAFLVSLI
                     AGTFLVTTEKSRHPVRRFFMGIVYLFKFLWEMIKANIHVALIVINPTLPIKPGIIKIK
                     SNLTKDSALTVLTNSITLTPGTLTVDINPETREIYIHCIEVESTAIDENTRSIGGRFE
                     KLIREVFE"
     gene            3631..3969
                     /locus_tag="PKX09_07380"
     CDS             3631..3969
                     /locus_tag="PKX09_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HOU17678.1"
                     /translation="MRVLRWVIGLVLIGAFMYANFFIYTASIYVKLINVILFSALFVI
                     YRVIVGPTAADRIVAVDILGILIIGMLALLGLNYDQSFFMDIGLIWALLSFIASLAFA
                     KVLEGRSLDD"
     gene            3962..4300
                     /gene="mnhG"
                     /locus_tag="PKX09_07385"
     CDS             3962..4300
                     /gene="mnhG"
                     /locus_tag="PKX09_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HOU17679.1"
                     /translation="MIRTLIGMAFILIGVTFDFLGVLGLVRLPDVYNRLQAATKCVTF
                     GSAGLLFGVFILQGFNSFGFKVLIGIVFIFLTSPVAAHVIARAAHRSGIKLTKETVVD
                     QYQEDHELKE"
     gene            4312..4914
                     /locus_tag="PKX09_07390"
     CDS             4312..4914
                     /locus_tag="PKX09_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU17680.1"
                     /translation="MFLPKLRELNEALRSLFSLPFTTKFPAKPHQPADGFRGFPEYHQ
                     EFCVGCGTCAQVCPTSAISVTDDIAAHKRILRVDYTSCAQCGQCQEKCITGKGIQLSK
                     NYLLAISDLKDQAVYNTVEKDLALCEACGTKIACFDQLLFVKERLGAKSYANPNLLLV
                     TQQQFSEIPDSHVKDRLRREDYVKMVCPKCRHKIVVKDEF"
     gene            4904..5371
                     /locus_tag="PKX09_07395"
     CDS             4904..5371
                     /locus_tag="PKX09_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOU17681.1"
                     /translation="MNFNDLKKTLAVYHSDRIIFVGLGNRDCGDDGASQILLNKLKHS
                     EEFKKAGFIFAGTNPENYLQKITDKNPQAVVFIDAAHFQDQPGTVKFLEAKQLKPSDF
                     STHAFSISLIADYLTYEKSCAVYYLGIQPENSGVGQSLSESVQSGLTHFFSES"
     gene            5382..6734
                     /locus_tag="PKX09_07400"
     CDS             5382..6734
                     /locus_tag="PKX09_07400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU17682.1"
                     /translation="MATNEPYKLILPFHTLETMQTRISWLVNLRWIGIISLLGIIPIA
                     EQVLKFSLGFPQIYTLAVVFIVLNVTYFFICKYFKFVNFGQEIAFTEIQIIFDFVLIS
                     FVVHYTGGIDNPFYFIYLVHIIISGILYQGIWPYINATVAAFLLTLWSGLEYFKVVNV
                     YSLSGSPAPLKVYLVSLAAFYILIYTATYVIKDIVSGYRMLKRVIDQKSELLEQTMLE
                     RDKMFRFTAHELKSPLTTIRSMLAVIDEVYSDSLQPELKEMVERAVRRTDQVLMMVKE
                     MIEVTQYRYGLMEQKLDRVNFQEWIDKVVEQQQIYAANKKIALIVKKLSQSPAIYIDT
                     VSMEKVLSNLINNAIRYSPAGAEVTILPFVKQQCFGFCVRDTGIGIAPDELSKIFEEF
                     YRSANAREMENLGTGLGLSLVKQIVEKYNGKIFVESKIGQGSTFTVEMPYLNLQINPK
                     "
     gene            6789..7211
                     /gene="nikR"
                     /locus_tag="PKX09_07405"
     CDS             6789..7211
                     /gene="nikR"
                     /locus_tag="PKX09_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOU17683.1"
                     /translation="MAGLIRFGVSLPEDLLTRYDNEIQRRGYSNRSEALRDLIREFLV
                     QKEVDSDAEVVGTLTLIYDHHVADLAAKLKSMQHESYQNIMANIHFHLDQHNCLEVIV
                     LRGRCSLIQNIAERLIGLRGVKHGKLTFTSTGKNLPGL"
     gene            7228..9093
                     /locus_tag="PKX09_07410"
     CDS             7228..9093
                     /locus_tag="PKX09_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU17684.1"
                     /translation="MTIKYTLKYFLLITLMAVSLSSAAIYTDTLKVFPFDPVVVTGSR
                     TAINRHELPVAITVIPENVLSEQSGAPLLDLLSENVPGLFITRRTNLGYGLSSGAGGQ
                     ISMRGIGSFPTTQVLVLIDGRPDIMGLFGHPLGDVYFAENVKKVEVQRGPASLLYGSN
                     AMGGAINIITDHKSEPGYHLRMPIRFGSFNSHQLNLHQSYERKNWGVSGTVGTVSSDG
                     YRTDGRDDYSNQNGIAEAHWQIGNAWRADVNAYFSNMEIYDPGQTDNPFSNHRYDIKR
                     RGGDLTFNHSLAKLTSDLKLHYNYGHHDIDDPNKYISDDYTAGLILTETYYKSEKSRV
                     MVGIDLRQYGGKAWLDSTWKKHNVAEQSLITQFNRNLFSLANLDCGLRYTHHSVAGAE
                     WIPALGMALHLPKGWYLKTQFARGYRNPTINELYLFMPSTTDLKPELSTNLEFIVEKE
                     IIGWLTTSLSLYHTKAENLIEKGWVSGMPIYQNRGEVVLKGIEWEGQLIITSNFSTSW
                     TMSVNTASQKVSGNPGQKCDLGLRWNPTEKLHLTLQSQWINNLYSLANPYSYGQPVYK
                     RLNGYWLLNWRGSYVINQFIDLSAEIENLTNVEYETMYHYPLPGRNFWIGITLKY"
     gene            9108..9707
                     /locus_tag="PKX09_07415"
     CDS             9108..9707
                     /locus_tag="PKX09_07415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU17685.1"
                     /translation="MNTAIYKNIIKYFDAQAASWHLSGLDRIQAENILSSVQLPESGV
                     VLDAGCGTGNLFPILRKIVPLATIIACDISRKMLTESRQRFSNNVPLWVGYCEELPLK
                     DASVDLILNYCIFPHIKNSYKALSEYRRVLKSGGHLLIVHPQGRQTINSKHRQIGFPI
                     SDDLLPPSAEIVERLESMSFRIREVVDREDLYLIEAEVA"
     gene            complement(9809..10528)
                     /locus_tag="PKX09_07420"
     CDS             complement(9809..10528)
                     /locus_tag="PKX09_07420"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOU17686.1"
                     /translation="MGSRIKTLLFLLSLLVILAMAKNPQDPDYYQLRQKMVNEQIIAR
                     GVRAESVIKAMQKVERHLFVPEQYRNFAYSDRPLPIGEGQTISQPYIVALMTELLDLK
                     KSDKVLEIGTGSGYQAAILAEICDSVYTIEIISSLGKQAQALLRELGYYNIHCKIGDG
                     YLGWPEHAPYDGIIVTCAPSKIPQPLKEQLAEGGRMVIPVGATYTQELVLVTKTKGKL
                     IQKSVIPVRFVPMLRSPNGLD"
     gene            complement(10559..11254)
                     /locus_tag="PKX09_07425"
     CDS             complement(10559..11254)
                     /locus_tag="PKX09_07425"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOU17687.1"
                     /translation="MSERKISGVKIAGLVLIVLGVLLLLDNLNLILFDLGDFLHNWWP
                     VFLILIGLNELLKTNHSGGWFLIGLGIILTLKINDIIDGSLLFALLLILIGIIIIIRP
                     RRFYPQKTADNSQPETNNSNNINLRAVFSESTENIISDNFSGGKIETIFGKMVVDLRP
                     AKMQPGRYHLNVETVFGQTILILPNEISAEVIGTPVFGRIDNQAKAIPRDESTAILEI
                     RAEVVFGKIEIRQ"
     gene            11445..13109
                     /locus_tag="PKX09_07430"
     CDS             11445..13109
                     /locus_tag="PKX09_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HOU17688.1"
                     /translation="MKKFILMLFMVLMMMNCDSKIKQTEQMAQAFIDSLTNVIKPLNK
                     AANLAYWEATASGKDEYYERYAGASLELATIYANPADFEKLKALKSSKIKDLLLARQV
                     EILYYRFLAKQIDTTLLKQITEMQTAVEARFNKFRGKIDGREVTGNDIKEILVSSNDL
                     TLRKKAWEASKQVAPAVEKDMLALVHIRNEAARKLGFNNFQQMSLITDEQDPAEIERI
                     FQELDAATREPFRHDKAMMDSILSKRFNIKPEELRPWHYADPFFQDVPAVSAVNLDQY
                     YQNQNVVKIAESFYAGIGLGVSQILANSDLYERPGKYPHAYCTDIDREGDVRIMVNVR
                     NNEEWMATVLHELGHAVYSINIDRQLPYFLREEAHLFTTEAIAMFFEKLSKNPTWMQA
                     MLNLDAEEEADIREATAQMLRLQKLIFARWSLVMYNFEKSLYENPEQDLNKLWWDLVE
                     QYQMIKRPENRNQPDWASKIHVCSYPVYYHNYQLGELLAAQLTEAIARYQGVNDGAKI
                     KYVNNPDLGNYLKTNIFSVGRKYRWDEMIVRATGEKLTAKYFVKQL"
     gene            complement(13224..15104)
                     /gene="pepF"
                     /locus_tag="PKX09_07435"
     CDS             complement(13224..15104)
                     /gene="pepF"
                     /locus_tag="PKX09_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOU17689.1"
                     /translation="MKQLFTFSVVIMLFLSNEETSVAQTRDRSQIPEKYTWDLTPIYP
                     SDEAYLEAKDALVKKFDQILNYKGKLGQSADQLYACLNLNSEISKEMARLGSYISFKA
                     DLDTRDTKYLGMRQELSQLATEYYTKAAFIQPEILEIDPAKLKKFIASKKELKVFEFY
                     INDLIRVKKHLLSEKEEKIIAETGLFADAPYTIYGLFSNAEFPFPEIVLSDGTKVKLD
                     LATFATLRALPVRADRELVFNSFFGKMYDFRRTFGATMDANVKQHLFIAHVRNYNSCL
                     ETALDANNIPTDVYTALIKNVNDNLPTFYRYLKIKQQLLGVDTLKYTDLYTPVVKDLD
                     IDYPIETAQQIILSALKPLGSDYLSVIERAFNERWIDVYPTPAKRSGAYSDGSIYDVH
                     PYVLLNYCNKYDDVATLAHELGHTMHSYFSNKTQPFPLSDYSIFVAEVASTFNEVLLF
                     HDNLNKVTDPDQKLTLLMNYLDGIKGTVFRQTQFAEFELRIHEMAECGEQLTGDSLTE
                     LYKQIVRMYYGHDKGICYVDDIINIEWAYIPHFYYNFYVYQYATSFTAATALAKKVLE
                     GDESARSNYLNFLSSGGSDYPINQLKAAGVDMTTAEPFNLTMQEMNWVMDEIEKVLTE
                     KK"
     gene            15551..16066
                     /locus_tag="PKX09_07440"
     CDS             15551..16066
                     /locus_tag="PKX09_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOU17690.1"
                     /translation="MIKSNINSSESKSPQPVRKTWDEYFLDIAEMVASRSTCFRNKVG
                     AVIVRDKVIVSTGYNGAPTNQPNCMEIGYCYRDRHQIKSGTQLERCRAVGSHAESNAV
                     VLAARNGQATAGATIYVVGHKSICDQCKAIIANAKIERVVMRNLDRQIEIIKPSVDWT
                     VHPVDQQNRNS"
     gene            16074..16661
                     /locus_tag="PKX09_07445"
     CDS             16074..16661
                     /locus_tag="PKX09_07445"
                     /inference="COORDINATES: protein motif:HMM:NF017538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HOU17691.1"
                     /translation="MLIYCHGFGSSGQATKAQILRTHFTDKMVLAPNLPWEPAGAIET
                     IIENIRNTRNNSRILLIGSSLGGFYALHLHQEYGVPAILLNPAIDPIGDTYPHDYNPC
                     DTENSSLAAWCREYTSQIRTLYHLPQTINPQNLFVFLNRDDELLDYRKAAEYFKVNNC
                     QVIVSESGGHRFLNFEELIPTIRAIYENLPPMNYI"
     gene            16854..16997
                     /locus_tag="PKX09_07450"
     CDS             16854..16997
                     /locus_tag="PKX09_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17692.1"
                     /translation="MAICDLEFGIWDFSHLAVLGELGGLIFQDQNKKNKTENGHWSLV
                     TGY"
     gene            complement(17150..>18939)
                     /locus_tag="PKX09_07455"
     CDS             complement(17150..>18939)
                     /locus_tag="PKX09_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU17693.1"
                     /translation="KPDFINNFIWIYELSRLKVLNIYITYKDNNLVTESESEFTEIIE
                     FRFKHKTGNWIYLQSVGNLLGKQLLFVSRDVTQRVQMEASLRENEERYRSLVENSAMG
                     IFIVDDNYTFEFVNQRLCQILGYTSSEITGHKFMEFLAPESVELVQDRYQRRQKREAV
                     PMNYEFEVIRKDGERRQVEIYSNVVKDSDGKNHTIAQILDITERVKAETARDSLESQL
                     HQAQKMEAIGRLAGGIAHDFNNILTIILGYSQMALMNLQPDQKFYSQFQKINDAARRS
                     ANMIGKMLAFSRQQNAEPQRLNLNTHLESTKGLLEKMVREDIKMSFIPGSRLHDIYLD
                     PTQFDQIIMNLVANAGDAMPKGGNLIIETANFTCDSEYSLKHHGFQPGNYVMISVSDT
                     GCGMDKETLEHIFEPFFTTKGEGEGTGLGLATVYGIIKQNNGFINVYSEPGKGATFKI
                     YFPVYEGRDEKPYQAEDLENIMGQETILLAEDDTDVRTFTRNALQQLGYQVIDFGEPI
                     EALEYFKKHSDEIDLLFTDLVMPGMNGQELHRQIEQIKPGIKVLFTSGYTSNIIAVQG
                     IIKRGINFLQKPFAPSVLGKKIRQILDTN"
BASE COUNT         5203 a         3672 c         4323 g         5741 t
ORIGIN      
        1 ctggtcaaaa acgttcggtt tgacgaaaat tatcttggcg ggatgccgtc tctggaaaaa
       61 ttccgcattg ccgggacgga gttttataat gaaatcagga atatgtctcc tttaaagtca
      121 atttacaatg cggctgaaaa gaagcatttt gatgtttacg atcttggggt gcgcggttct
      181 ttgagttttt ccggacttct aaagcgcctg catagcggcc aattgctgga ctataatctc
      241 tggattatta tcgggatgtt aattctgcta tgggtggcgt ggtaatgttg acgattgaat
      301 attttatgga ggaataaatg ccgactgaaa cgtggttgat ttttatcttg ggttttatgt
      361 tagtaggttc gattgccgcg ctcgagatca aggatttgct ctcagcgatc attgcggtgg
      421 gaattgtcgg gttgggactg tctatggcat ttttgctttt acaggcgccc gacctggcaa
      481 ttgtccagtt tctgttcgag atttttgcga tagtgataat ggtgcgagcc tttatgaaac
      541 cgggatatca tttcagcgat acgaaatcca aacatagggg tagaacgatt atcgtaatgg
      601 cagttctagt tgccattttt atattaagca tcaatgtttt caaagctttg ccgccttttg
      661 gcgagccact gatgaccact tcgcaacact atctggaaaa tgctgccaga gaaaccggtt
      721 cggccaatat cgtggcttcg atagttctgg attaccgcgg ttatgatacg ctgggagaag
      781 ccaccgtatt attcgtagcg gttttaggaa cgctgactat tctaagggtg aaatcgaata
      841 agaaaaaaga agaggaaagc gaaaaatgaa acaagaacct ggtttaacat taatcgccaa
      901 aaccgtgacg cgggtcagtg tctggttgat cattctctac ggaatctata taattttgca
      961 cggtcatttg agtcccggcg gcggttttgc cggcggggta gtgatcgcgc tagcatttct
     1021 caatgtgatg ttaacctatg gtcgcgaaac cgttacgcac tgggtcaatg tcgaatttct
     1081 gcacgatttg gaaccagcct gcggtgcgat atatatggcg attggcattt taggaatttc
     1141 tatcagcggc ggttttttag ccaatttttt aagtaaaggg acgcttttta atctgctcag
     1201 ttccgggaat attctattgt taaatattat tatcgggatc aaggtggcta tgggattatt
     1261 caccgtggtc tggacactgg cgattttcca tataaacgag ggaggtgaaa aatgatcgtt
     1321 tatctgttat gttttgcatt attacttgtc ggtatttacg gtttgctggt taaacgcgac
     1381 ctggttcgtc ttattattgc atctgggatt acgggctacg ccacgaattt gatgctggtt
     1441 ttattcggat acaaaagggg agcgatttat ccgattttgg aaaaaggcaa agccgccggc
     1501 gagcggataa tggttgaccc tttgccgcaa gcgttggtgt tgacttcgat cgtcattgaa
     1561 ctgggtataa cggctatgct ggtagcgctt gccatccgat tattcgagaa aaataaaaca
     1621 tttgatatta ctaaaatcaa gaggttatca ggatgatcct cccatttttt attataattc
     1681 cattattaac ggcttttata attaccttga tcgccggaga gcgagacaat tgggcggtaa
     1741 ttctctcgat tcttgctgtg gtttctcttg tgattctttc ggtatattgt ttcgtatcgt
     1801 tgaatgggcg cactctggtt tatgaaatgg gtcgctggca agcgccactg ggcattattc
     1861 tggcagttga tgctctttca gcctttatgt tggtgattgt cagtattatt gcgctaacgg
     1921 cgattatgtt ttcggtgcag tatattagaa atttatcgcc gtcctggaag tattacgctt
     1981 tatttatgtt gctgacgggt ggaatgaacg gcgttatagc gactggcgat ttattcaatc
     2041 tgtttgtttt tgtcgaaatc acgcttttcg cggcttacgg gctggtggct tatggtaacc
     2101 gcgcggagga atttgaggcg tccttcaaat atgctattat gggttcaatt tcatcaacct
     2161 tagtgctagt aggtattggg ataatttatt cggcaacctc aaccttgacg atggctttga
     2221 tcgctgagcg tttgccgcaa aatccaaccg tgatgtattg gagcggtgga ctatttgtgg
     2281 cggctttcgg gctgaaagcc gctattatgc cgtttcacgc ctggttgccg gatgcacatt
     2341 cttccgcacc ggcgccgatc tcggcgatgc tttccggggt attgataaaa gccctgggtg
     2401 tgtatgtgct tatcaggctt tttttcaatg tctttggcga gatgccagtg ttcagcgaag
     2461 tttttctagt tctgggtacc gtttcgatgg ttataggggt tttcttagct ctggggcagt
     2521 gggatgttaa acgtctgctt gcctatcata gcattagcca gatcgggtac attattatcg
     2581 gtgtggggtt aggaacgact ttaggtctat taggtgctgt ttttcatctt ttgaatcacg
     2641 ccatattcaa agctttgttg tttttgaatg ccggttcgct ggaaatggca ttgggtacgc
     2701 gtgatttgcg tcagatgggc aaagtttcca aagctttgcc ggtgacgtcg tttacttcga
     2761 tggtggcttc gctttcgatt tcaggaatac caccgttcaa tggtttcttt agcaaattga
     2821 taattttcat cgccgccatt cagatcggcc gcccggtggt ggcgttttta gctggactcg
     2881 gcagtttgct gacgttagct tcatttatga aagttcaacg ttatggtttt catggcgaga
     2941 aaacagttac tgccgatcaa gctaaagtag gatggggtat gcgagcggct atgattgtgc
     3001 tggcgatact ttgtctggca accagtgctc tggtgattcc tggtgtccgg gaagtgaccc
     3061 tcgatccggt ggtagaagtg attatgaata aaaccggcta cataacgcgg gtgttaggga
     3121 ggtgaaaatg accatcaaga atcgtagtcg cgttattgtg tttgtgcttt cgtttttggt
     3181 ttggttggca ttgacagata taaaagacat acaggaagta gtcgcaggct taatagtggc
     3241 atttctggtt agtttaatcg ctgggacatt tctggttacg acggaaaaga gccggcatcc
     3301 ggttcgacgc ttttttatgg gaattgtcta tttattcaaa tttttatggg aaatgattaa
     3361 agccaatatt cacgtggcat tgattgttat caaccccact ttgccaatta agcctggcat
     3421 tatcaaaatc aagagtaatt taaccaaaga ttcggcctta actgtattaa cgaattccat
     3481 cacattgaca ccgggtactc tgacggtgga cattaatcca gaaactcgtg aaatttacat
     3541 tcattgtatc gaggttgaat caaccgctat tgatgaaaat actcgttcca tcggcgggcg
     3601 ttttgaaaaa cttatcaggg aggtttttga atgagagttc tgcgatgggt gattggattg
     3661 gttctaatcg gcgcctttat gtatgctaat tttttcattt atacggcttc gatttatgtc
     3721 aagttgataa atgtcattct tttcagcgct ttgtttgtga tttaccgagt tattgttggt
     3781 ccgacagccg ccgatcggat tgtagcagta gatattctgg gcatattgat tattggcatg
     3841 ctggcattac tgggcttgaa ttacgatcaa tcttttttta tggatattgg attgatctgg
     3901 gcacttttaa gctttattgc ctcgctggcg tttgccaaag tattagaagg gaggtcgctc
     3961 gatgattagg acattaatcg gtatggcgtt catcctgatt ggggtaactt ttgattttct
     4021 gggagtatta gggctggtgc gtttgccgga tgtttataat cgtctgcagg cggctaccaa
     4081 atgcgtgacg ttcggctcag ctggtttact atttggagtt ttcattctcc agggcttcaa
     4141 tagtttcggt tttaaagtgt tgatagggat tgtatttatt ttcctgactt cgccagtggc
     4201 ggcgcacgtc atagcgcgag cggcacatcg cagtggcatc aagttgacta aagaaaccgt
     4261 cgttgatcag tatcaagaag accatgaatt aaaggaatag gggagactgt tatgtttctg
     4321 ccgaaattaa gagaattaaa cgaagccttg cggtcgcttt tttcattacc atttactact
     4381 aagtttccgg ctaaaccgca tcaaccggcg gatggtttcc gcggattccc ggaatatcat
     4441 caagaatttt gcgttggttg cggcacctgc gctcaggttt gtccgacgag cgctatttcc
     4501 gttacggacg atattgccgc tcataagcgt attctgcgag tcgattatac ttcctgtgct
     4561 cagtgcggac agtgccagga aaaatgtatc acaggtaagg gcattcaact ttcgaaaaat
     4621 tacttgcttg ctatctcgga tttaaaagat caagccgtct ataataccgt ggagaaagat
     4681 ctggctcttt gtgaagcctg cggcactaaa attgcctgtt tcgatcaact actttttgtt
     4741 aaagaacgct taggcgcaaa atcgtacgct aatccaaatt tgcttctggt aactcaacaa
     4801 caatttagcg agatacccga ctcgcacgtg aaagaccgcc tacgccgcga agattacgtt
     4861 aaaatggttt gtcccaaatg tcgccataaa atcgtcgtaa aagatgaatt ttaatgattt
     4921 aaagaaaact ttagccgttt atcatagcga ccgaataatt tttgtaggat taggtaatcg
     4981 ggattgcggt gatgatggcg cgagccagat tttgctaaat aaattaaagc acagtgaaga
     5041 atttaaaaaa gcgggattca tttttgcagg aactaatccg gaaaattatt tgcaaaaaat
     5101 cactgacaaa aatccgcagg ctgtggtctt tattgatgcc gcccattttc aggaccagcc
     5161 cggaacggta aaattcctgg aagcgaaaca gctgaaaccg tccgatttta gtactcacgc
     5221 cttttcgatt agcctaatcg ccgactattt gacttacgaa aaatcctgtg cggtttatta
     5281 cctcggaatc cagccggaaa atagcggagt cggtcaatcg ctatccgaat cggtccaaag
     5341 cgggctaacg cattttttct cagaaagtta gaaatataaa tatggctacc aacgaaccct
     5401 ataaattaat tttgcctttt catacgcttg aaacgatgca gactagaatc agctggctgg
     5461 taaacctgcg ctggataggc ataatcagtt tattagggat aattccgatt gccgaacaag
     5521 ttttgaaatt cagtctggga tttccgcaga tttatactct ggcggtggtt tttattgttc
     5581 tcaatgtgac ctattttttt atatgtaagt atttcaaatt tgtcaatttc gggcaggaaa
     5641 tcgcctttac cgaaatccag ataatttttg attttgttct gatttcgttt gtggttcatt
     5701 atacgggcgg tattgataat ccgttttatt tcatttacct ggtgcatatt atcatttcag
     5761 gaattcttta tcagggcatt tggccttata tcaatgccac cgtagcggcg ttcttgttga
     5821 ctttgtggtc gggactcgaa tatttcaaag tagtcaatgt ctattcgctg agcggttcgc
     5881 cagctccttt aaaagtatat ttggtatcct tagccgcctt ctatattttg atttacacgg
     5941 ctacctacgt aatcaaagat attgtttctg gttaccgaat gctgaaaaga gtaattgatc
     6001 aaaaaagcga attgctcgaa caaactatgc tggaacgcga taaaatgttt cgtttcacgg
     6061 cgcatgaact caaatcaccg ttgactacta ttcgcagtat gctggctgta attgatgaag
     6121 tttattctga cagtctccaa ccggaattaa aggaaatggt cgagcgagct gtacgacgca
     6181 ctgatcaggt tttaatgatg gtcaaagaaa tgattgaggt aacgcagtac cgatatggct
     6241 tgatggaaca aaaattagat agggttaatt ttcaagaatg gattgacaaa gtcgtcgaac
     6301 agcaacagat ttatgccgct aataaaaaga ttgcattgat agtcaagaaa ttatctcaat
     6361 caccagcgat ttatatagat acggtcagta tggagaaggt gctttccaat ttaatcaata
     6421 atgccattcg ctatagcccc gccggagccg aagtaacgat cctacccttt gtcaaacagc
     6481 aatgtttcgg attttgtgtc agggataccg ggatcggtat tgccccagat gaactgtcta
     6541 aaatttttga ggaattctat cgcagtgcta atgctcgtga aatggaaaat ctggggaccg
     6601 gtttaggatt gagcctcgtc aaacaaatcg tcgaaaaata taacggcaaa atatttgtcg
     6661 agagtaaaat agggcaaggg agcaccttta ccgtcgaaat gccctattta aatttgcaaa
     6721 ttaatccaaa atgatatatt ttttgactcg ttgtgttaca aatttgaaaa tgttgctacg
     6781 aggtgaatat ggctggcttg attagattcg gagtttctct accggaagac ctgctaacac
     6841 gctacgacaa tgaaattcaa cgcagaggct actctaatcg ttcggaagcc ttgcgtgacc
     6901 taattcggga atttttagtc cagaaggaag ttgattccga tgccgaggtt gtcggaacat
     6961 taaccttgat ctatgatcat cacgttgctg atttggcggc aaaactgaag tctatgcaac
     7021 acgagtcata ccagaatatt atggcgaata ttcattttca tctcgatcaa cataattgtc
     7081 tcgaggtaat cgtattgcgc ggtcggtgtt cattaataca gaatatagcc gagcggctga
     7141 tcggtttgcg cggggtaaaa catggcaaat tgacctttac atcaacagga aaaaacctac
     7201 cgggtttata acatcagaga ttaaaaaatg acaattaaat atacgttaaa atatttctta
     7261 ttaattacct taatggcagt gagtttaagc tccgcggcga tttataccga tacgctgaaa
     7321 gtttttccat tcgatccggt agtggtaacc ggatcgcgca cggccatcaa tcggcatgaa
     7381 ctgccggttg ctatcacggt catccccgag aatgtattga gcgaacaaag tggagcgccg
     7441 cttctggatt tgttatccga aaatgtgccg ggattgttta tcacgaggcg gacgaatctt
     7501 ggatatggac tgagtagcgg tgccggtggt cagatttcga tgagaggtat cggtagtttt
     7561 ccgactactc aggtattggt tttgatagat ggccgcccgg acattatggg acttttcgga
     7621 catcccctgg gcgatgttta ttttgctgaa aatgtcaaaa aagtcgaggt tcaacgcggt
     7681 ccggcttccc ttttatatgg ttcgaatgcg atgggaggag cgattaatat catcacggat
     7741 cataagtccg agccgggtta ccatttgcgg atgccaatac gattcggcag ttttaattca
     7801 catcaattga atctgcatca gtcgtacgag cgaaaaaact ggggagtctc gggaacggtc
     7861 ggaacggtta gcagtgatgg ttatcgaaca gatggtcgag acgattattc caatcaaaac
     7921 ggcatagccg aggctcattg gcaaattggc aatgcctggc gggcagatgt caatgcctat
     7981 ttttcgaata tggaaattta tgatccgggg caaactgaca atccttttag caatcatcgc
     8041 tatgatatca aacggcgcgg tggcgatctg acttttaatc acagtctggc gaagttaacc
     8101 agcgatttaa aattgcatta taattatggg catcatgata ttgatgatcc gaataaatac
     8161 atatcggatg attacacagc cggtttgata ttgacggaaa catattataa atctgaaaag
     8221 tcacgagtaa tggtcgggat agatttgcgt cagtacggcg gcaaagcctg gttggattcg
     8281 acctggaaaa agcacaacgt cgctgaacaa agccttatta ctcaatttaa tcgaaattta
     8341 ttttcattgg ctaatctcga ttgtggattg cgctatactc atcattcggt cgctggtgca
     8401 gaatggatac cagccctcgg tatggctctc catctaccaa aaggttggta tcttaaaact
     8461 caatttgcca gaggctatcg caatccgacg atcaatgaac tttatttatt tatgccgtca
     8521 accaccgatt taaaaccaga actatcgacc aacctggagt ttatcgtcga gaaggaaatc
     8581 attggttggt taacgacgtc tctatcatta tatcatacga aagccgagaa tttgattgaa
     8641 aagggctggg tctccggtat gccgatttac caaaatcggg gtgaagtggt tctaaaaggt
     8701 atcgaatggg aagggcaatt aattatcacc tctaattttt ccacttcctg gacgatgagt
     8761 gtcaataccg cttcccagaa agttagcggc aatcccggtc aaaaatgcga tctcggtttg
     8821 cgctggaatc cgaccgaaaa gctacatttg actttacaga gccaatggat taacaatcta
     8881 tatagcctcg caaatccgta cagttatggt cagccagtct ataagcgcct aaacggttat
     8941 tggttattaa actggcgcgg tagttacgta atcaaccaat tcattgatct aagcgccgaa
     9001 attgagaacc tgaccaacgt ggaatatgaa actatgtacc attatccttt gccaggccgc
     9061 aacttttgga ttgggataac cttgaagtat tgatttattt tagacagatg aatacggcaa
     9121 tttataagaa tataattaaa tatttcgatg cccaggcggc tagctggcat ttatccggat
     9181 tggatcggat tcaagccgag aatattttga gttcagtcca attacctgaa tccggagtag
     9241 tgctggatgc cggttgcgga actggaaatt tatttccgat attgagaaaa atcgtaccgc
     9301 tggctactat tattgcctgc gatatttcca gaaaaatgct gaccgaaagc cgtcagcgtt
     9361 tctcgaataa tgtaccactc tgggtcggat attgcgaaga gttgcccttg aaagacgcca
     9421 gtgttgatct aatcctgaat tattgtattt ttccgcatat taaaaacagt tataaagcgt
     9481 tatctgaata tcgccgcgtt ttaaaatccg gcggacatct actgatagtt catccacagg
     9541 gtcgccagac gattaattcc aagcatcgtc aaatcggatt tccaattagc gacgatttat
     9601 tgccaccttc agccgaaatt gtggaacgtc tggaaagtat gtcatttcgg attcgcgagg
     9661 tcgttgaccg agaagatcta tatctaattg aagccgaggt tgcttaaccg caaagaacac
     9721 aaagtgcgct gtgggaattg ccagtatatc ttaaatgaac tatactgctg atggtaaata
     9781 gctttcgagt ggtaatttca actgtgtttt aatctaagcc gtttgggctt ctcagcatcg
     9841 gcacaaagcg caccgggatg actgattttt ggattaattt gcccttagtt ttagtgacca
     9901 gaactagttc ctgggtataa gttgcaccaa ccggaataac cattcggcca ccctcggcga
     9961 gttgttcttt gagtggttgg ggaattttag atggcgcgca ggtgacgatt ataccgtcgt
    10021 acggcgcatg ttccggccag ccgagataac catctccaat tttgcaatgg atgttgtaat
    10081 agcccagctc tctaagaagt gcctgagctt gctttccgag tgaggagatg atttcgattg
    10141 tataaacgct atcgcagatt tcagccagaa ttgccgcctg ataaccactg ccggtaccga
    10201 tttcgaggac tttgtcgctc ttttttaaat ccagcagttc ggtcatcaga gctacgatat
    10261 aaggttgcga gatagtttgc ccttcgccaa ttggcaaagg acgatcggag taagcaaaat
    10321 tccggtattg ttcaggcaca aatagatgtc gttcaacctt ttgcatcgct tttatgacgg
    10381 attcagcccg gacaccgcgt gcaataattt gctcattcac cattttttgc cgtaattggt
    10441 aataatcggg atcttgggga ttttttgcca tagccaatat taccaaaaga cttaataaaa
    10501 atagtaaggt tttaattcta ctacccattt ttctatcaat gaatcctgac tattcacatt
    10561 attggcgaat ttctatcttg ccgaaaacta cttcagcccg aatttctagt atcgctgtgc
    10621 tttcgtctct cggtatggct ttcgcttgat tgtctatccg tccgaaaacc ggcgtgccga
    10681 ttacttcagc tgaaatttca ttaggtaaaa tcaggatcgt ttgaccgaac accgtttcga
    10741 cgttgaggtg atagcgcccc ggttgcattt tagctgggcg caagtcaact accattttcc
    10801 cgaaaatagt ttcaattttg ccgccactga agttgtctga gataatgttc tcggtggatt
    10861 cggaaaatac agctcgtaag tttatgttat tgctattatt ggtctcgggt tgagaattat
    10921 cggcggtttt ctgaggataa aatcggcgag gacgaattat tatgattatt ccgattaaaa
    10981 tcaatagcaa tgcaaataat agagaaccgt caataatatc attgattttc aatgtcagaa
    11041 ttattcccag tccaattaaa aaccaaccgc cggagtgatt cgtcttgagt aattcgttca
    11101 aaccgattag aatcaaaaat acaggccacc aattatgcag gaaatcgcct aaatcgaaaa
    11161 gtatcagatt tagattatcc agaagtagta aaactcctaa aacgataaga accaatccgg
    11221 ctatctttac tcccgatatt ttgcgctcac tcataataaa cctttctctc tttttttcaa
    11281 ttatacgaaa tagtttaaac taaatcaatc ggaaaatcgg ttaattttga ttggttaatt
    11341 cttgaatcct cgtgacttta atggtaaatt aaaaaccaaa tatgcacgat gaatattata
    11401 acaagatgta tcagtagaaa atatctgcga ggagtttatc cgatatgaag aaatttatct
    11461 taatgttatt tatggtgtta atgatgatga attgtgacag taaaattaaa cagaccgaac
    11521 agatggcgca ggcttttatt gatagcctga ccaatgttat taagccgttg aataaagcgg
    11581 ctaatttagc ctactgggaa gccactgctt ccggaaaaga tgaatattac gaacgctatg
    11641 ccggtgcttc tttggaattg gcgacgattt atgccaatcc agccgatttc gaaaagctaa
    11701 aagccttaaa atccagcaaa atcaaagacc tattattagc gcggcaagtc gagattctct
    11761 actatcgctt tttagccaaa caaattgata caaccttgtt aaagcaaatt accgaaatgc
    11821 aaacggcggt tgaagctcga ttcaataaat tccgtggtaa aattgacggt cgcgaagtca
    11881 ccggcaatga tatcaaggaa attctggttt cgtcaaacga ccttacatta cggaaaaaag
    11941 cctgggaagc ctccaagcag gttgccccgg cagtcgaaaa agatatgctg gctctggtgc
    12001 atatccggaa cgaagccgct cggaaactcg gattcaataa ctttcaacag atgtcgctga
    12061 ttaccgatga acaagatcca gctgagatcg agcgaatttt ccaggaactg gatgccgcaa
    12121 cgagggaacc attccgtcat gataaggcta tgatggattc gattctgtcc aagcgtttca
    12181 atatcaaacc ggaagaacta cgtccctggc attatgccga tccctttttt caggatgttc
    12241 cggctgttag cgcggttaac ctcgatcaat attatcagaa tcagaatgtg gtaaaaatag
    12301 ccgaatcatt ttatgctgga attggtctgg gtgtatcgca aattctggct aatagtgatt
    12361 tgtatgagcg tccgggcaaa tatccgcatg cctattgcac tgatattgat cgtgaaggcg
    12421 atgtgcgcat tatggtaaac gtgcgcaata atgaagaatg gatggcgact gttctacatg
    12481 aactggggca tgcggtttat tcgatcaata ttgatcggca actgccttac tttttacgtg
    12541 aagaagctca tctgttcacg accgaagcca ttgcgatgtt ttttgagaaa ttatcgaaga
    12601 acccgacctg gatgcaggca atgcttaacc tcgatgccga ggaggaagcc gatattcggg
    12661 aagcaaccgc gcagatgtta agattgcaaa aactgatttt tgcccgctgg tcgttggtga
    12721 tgtataattt tgaaaagtct ttatatgaaa atccggaaca agacttgaat aaactctggt
    12781 gggacctcgt tgaacaatat caaatgatca agcggccgga aaatcgcaac cagccggatt
    12841 gggcttccaa gattcatgtt tgttcttacc cggtttatta tcataattac cagctaggtg
    12901 aacttttagc cgcccaattg accgaagcga tagcgcgcta tcagggagtt aacgatggcg
    12961 ctaaaattaa atatgtcaat aatcccgatc tgggaaatta ccttaaaacc aatatttttt
    13021 cagtcggcag aaagtatcgc tgggatgaga tgattgtccg agctaccggc gaaaagctga
    13081 ccgccaagta ttttgtcaag cagttgtaag ccctaataag tgctaataag aaattaaaag
    13141 accctgtgat attgagttac attcacgcac ttatgcattt aataagcttg taattaatat
    13201 ttctttgtag aacttaaaag cacttatttc ttctcggtga ggactttttc aatttcatcc
    13261 attacccaat tcatttcctg cattgttaga ttgaacggtt cggcagtagt catatcaacc
    13321 ccggcggctt ttaactggtt gatcgggtag tcggaaccgc cggaagagag gaaattcaga
    13381 taattggacc gggcggattc gtcaccttct aatacttttt tggctaacgc ggtagcggct
    13441 gtgaaggagg tggcatactg ataaacgtaa aaattgtaat agaaatgagg aatgtaagcc
    13501 cattcgatat tgatgatgtc atccacgtag caaatacctt tgtcgtggcc ataatacatc
    13561 cgcacgattt gtttataaag ttcagttagt gaatcaccgg tcaactgttc accgcattct
    13621 gccatttcgt gtatgcgtag ttcaaactcg gcaaactgcg tctgacgaaa aacagtgcct
    13681 ttaatgccgt ctaaataatt catcaacagg gtgagtttct gatcgggatc agtgacttta
    13741 ttgagattat catgaaaaag cagaacctcg ttgaaggtcg aagcaacttc agcgacgaaa
    13801 atcgagtagt cagaaagcgg aaaaggttgg gttttattgg aaaagtagct gtgcatagtg
    13861 tgaccgagtt cgtgcgccag ggtcgcgacg tcatcgtatt tattacagta attgagcaaa
    13921 acgtagggat gcacgtcgta aattgagccg tcggaatagg cgccactccg tttagcagga
    13981 gttgggtaaa cgtcgatcca acgctcgtta aaagcccgct caataacgct gagatagtca
    14041 cttcctagcg gcttcagagc gcttaaaatt atttgctgag cagtttcaat gggataatcg
    14101 atatccaaat ccttgacaac tggtgtgtag aggtcggtat atttcaatgt atctacaccg
    14161 agcaactgct gtttaatttt aaggtaacga taaaaagtcg gtagattgtc attaacgttt
    14221 tttatcaaag ccgtatagac atcggtagga atgttatttg catcgagggc ggtttccaaa
    14281 caactgttgt aattacgaac gtgagcgata aaaaggtgct gtttgacgtt ggcgtccata
    14341 gttgccccga aggtgcgacg gaaatcatac attttgccaa agaaagagtt gaaaactaat
    14401 tcgcgatcgg ctcgcactgg cagagcgcgc aaggtagcaa aggtagccag atcgagtttg
    14461 acttttgtgc cgtcgcttaa aacgatttcc gggaaaggga attcagcatt gctaaaaaga
    14521 ccgtagatcg tatagggcgc atcagcgaat aagccggttt cggcgataat tttttcttct
    14581 ttttcggaaa gcagatgctt tttaactcgg atcaggtcat taatataaaa ctcgaagact
    14641 ttcaattctt tttttgaggc gatgaatttt ttcagcttgg ctggatcaat ttcaaggatt
    14701 tcaggttgga taaaagccgc cttcgtataa tattcggtcg caagttgcga gagttcctgg
    14761 cgcatgccca ggtatttcgt gtcgcgggta tcgaggtcgg ctttgaaact gatatatgag
    14821 cccagtcgcg ccatttcctt gctaatttcg ctattgaggt taaggcaggc atagagttgg
    14881 tcagccgact gcccaagttt gcctttgtaa ttcaaaatct ggtcaaattt tttaacgaga
    14941 gcgtctttgg cttctaaata ggcttcgtct gatgggtaaa tcggtgtcaa gtcccaggta
    15001 tatttttccg gaatctggga gcggtcgcgc gtctgcgcaa cggaagtttc ttcattagat
    15061 aagaatagca taatcaccac gctgaaagta aatagttgtt tcatttttat cctctttgtt
    15121 ttaatagtta cctattatat ttgaagcgga aatgctgatt tggtttctga aaatatttga
    15181 tttagatgga gaaaccttac tccatttaaa ttggttttaa cgttccggaa tatattaaga
    15241 gtaaagaaaa taataccaaa aatcacaatc aaaatttagg aaaaatagag gtaatttgcc
    15301 agacaaatct cgacaattgg ttattgattg gtagattgga taggtggaga gactttcgat
    15361 atttttctta gctatagact tctcaggtca acgctcggaa tttgaaataa agtaggtcgt
    15421 tacccttcga cgagctcagg gtgacaaaga agcggcgagc tcagggtgac atttagagtt
    15481 agcctaataa tgattttctt catttctggt ttgtgctttc gtatatttcg ctaaaattga
    15541 ggatggtaaa atgataaaga gcaatataaa ttcatcggag tcgaaatcac ctcagcctgt
    15601 tcgtaaaaca tgggatgaat atttcctgga tattgccgaa atggtggcaa gtcgctcaac
    15661 ctgcttccgg aataaagtcg gtgcggtgat tgtgcgcgac aaagtgatcg tctccaccgg
    15721 ctataacgga gcgccaacca accagccgaa ttgtatggaa atcggttatt gttaccgcga
    15781 tcggcatcag attaagtccg ggacacagtt ggaacgttgt cgggcggtgg gttcacatgc
    15841 tgaatccaat gccgttgttt tggcggctcg taatggtcag gctaccgccg gtgctactat
    15901 ttatgtcgtt ggtcataagt ctatttgcga tcagtgtaaa gcaattatcg cgaatgccaa
    15961 gatcgaaagg gttgttatgc gcaacttaga tcggcaaatc gaaattatta aaccgtctgt
    16021 tgattggacg gttcatcctg tagatcaaca aaatagaaat tcttgacgct tatatgctga
    16081 tttattgtca tggattcggc agctcgggtc aggctactaa agcccagatt ttacggacgc
    16141 attttaccga taaaatggta ttagcgccga atttaccttg ggagccagca ggggcgattg
    16201 aaacaataat cgaaaatatc cgaaacacca ggaataattc cagaattctt ttaatcggat
    16261 cgagtttagg gggattttat gccttacatt tgcatcagga atatggtgtt ccggcaattc
    16321 tgcttaaccc ggcaattgat ccaatcggtg atacttaccc acatgattat aatccttgtg
    16381 atacggaaaa tagctcgctg gcggcttggt gtcgggaata tacttctcaa attcgcacgc
    16441 tttaccactt accccaaaca ataaatcccc agaacctatt cgtgtttctc aatcgggatg
    16501 atgaactgct ggattataga aaagcggcgg aatattttaa ggtaaataat tgtcaggtga
    16561 tagtttccga gagcggcggt catcgctttc tgaattttga ggaattaatc ccgactataa
    16621 gagcgattta tgaaaatctg ccaccgatga attatatctg attgaatttt ataattacaa
    16681 cgggcttttt tggtcattgg tcattagtca ttggtcatta gtaaagaggt cgagaaaaga
    16741 gaaaagaggt tgagaaaaaa gactgagaaa ggtcattggt cattagtcac tggtaaatag
    16801 gctgagaaaa gagactgaga aaaggtacag gagacagttt tgcggaaatt agtttggcta
    16861 tttgtgattt ggaatttggg atttgggatt tttctcattt agcagttctt ggcgaacttg
    16921 gcggtttaat cttccaagac caaaacaaga aaaataaaac tgagaatggt cactggtcac
    16981 tggtcactgg ttattagtca ttggtcatta gcacttttta tttatcatta aaatacgctg
    17041 cattttatct aatcttagtc ttaacgaaaa aatttcctta ctttgttgta aaaaggtaaa
    17101 atattcacgt aaactagaat tactattcac gaattaatcg ctacaacttt taattcgtgt
    17161 ctaatatttg gcgtattttt ttccccagga ctgatggcgc aaaaggcttt tgcagaaaat
    17221 ttatacccct tttaataata ccctgaacgg caattatatt agaggtgtat ccggatgtaa
    17281 agagcacttt aatgccgggc ttaatctgtt caatttggcg atgcagttcc tgcccattca
    17341 ttcccggcat gaccaaatcg gtgaacaata aatcaatttc gtcactgtgt tttttaaagt
    17401 attccagcgc ttcaatcggc tcgccaaaat ctataacttg ataccctaat tgttgcagtg
    17461 catttcgggt aaaagtgcga acatcggtat catcctccgc cagcaggatt gtctcttgtc
    17521 ccattatatt ttccagatct tccgcttgat aaggtttttc gtctcgacct tcataaacgg
    17581 ggaaatagat cttgaatgtg gcacctttac ctggttcgct atagacgtta ataaatccat
    17641 tgttttgttt gataattccg taaaccgtag caagcccaag gccggttccc tcaccttctc
    17701 ctttggtcgt aaaaaacggt tcgaatatat gttcgagggt ttctttatcc attccgcaac
    17761 cggtatcact gacagaaatc atgacataat tgcccggttg aaagccgtga tgcttcaggg
    17821 aatattcgga atcacaggtg aaattagccg tttcaataat cagatttcca cctttaggca
    17881 tggcatcacc ggcattggct accagattca taataatttg atcgaattgg gttggatcca
    17941 gataaatgtc atgaagccta gatccgggaa taaaactcat tttaatgtct tcgcgcacca
    18001 ttttttccaa cagacctttg gtagattcca gatgagtatt cagatttagt ctttgaggtt
    18061 cggcattttg ctggcgcgag aatgccagca ttttgccaat catattcgct gaacgacgag
    18121 cggcatcatt aattttttga aattgggagt agaatttttg atctggctgt aaattcatta
    18181 acgccatctg cgagtagccc agaataatag ttagtatatt attaaaatca tgggcgatac
    18241 cacctgccag ccgtccaatt gcctccattt tctgagcttg atggagttga gattccagac
    18301 tatcccgtgc ggtttcggct ttcacccgtt ccgtaatgtc gagaatctgg gcgatagtgt
    18361 ggttttttcc atcagaatct tttaccacat tggaataaat ctcaacctgg cgtctttcgc
    18421 cgtccttgcg aattacctca aattcataat tcatcggaac cgcttccctt ttttggcgac
    18481 gctgataacg atcttgtacc aattcgacac tctccggtgc taaaaattcc ataaacttat
    18541 gtccggtaat ttcggatgag gtatatccca aaatttgaca gagccgttga ttgacaaatt
    18601 caaaagtata gttatcgtca acaataaaaa ttcccatagc actgttttcc accaatgagc
    18661 gatatctttc ttcattctca cgcaaagagg cttccatctg tacgcgctga gtgacgtctc
    18721 ttgatacgaa taacagctgc tttcccagga gattgccgac actctggaga taaatccaat
    18781 tgcccgtttt gtgtttgaag cgaaactcga tgatttcggt gaattcagat tcactctcag
    18841 tgaccaaatt attatcttta taagtaatat aaatatttaa tacttttagc ctgcttaatt
    18901 cataaatcca tataaagttg ttgataaaat ccggttttg
//
LOCUS       DAOSMP010000040        18263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_3063_length_18263_cov_15.969409, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000040 DAOSMP010000000
VERSION     DAOSMP010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 18263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18263
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3063_length_18263_cov_15.969409"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1824)
                     /locus_tag="PKX09_07460"
     CDS             complement(130..1824)
                     /locus_tag="PKX09_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HOU17694.1"
                     /translation="MFQSIKKSIPIWAIINLIWLGIFMTLSNVTCGDKVDNSKADLAY
                     VKAELNKLAPVELKCDLSSLSVADRQVLVRLVETGRIIDQLFLIQVNPENPEILAKLQ
                     TSTDENDLPYLELFTIMFGPWNRIDHNKPFIGKKEKPLGAGFYPEDMTREEFEKFIAE
                     HPEQKKAFESEFTIIKRQKGQLVAIPYHQEYQPYINAISNLLTEAAEITQDPTLKKYL
                     NQRADDLKTDDYFQSDMDWMDLAGDLEIVIGPYEVYEDNLFNYKAAYEAFICMVDHAE
                     SEKLAKVTKYLDEIEANLPLAKEYRNYSRGKVSPIKVVQEIFSAGDTKAGVQTTAFNL
                     PNDERVRSAKGSKKVMLKNVAEAKYEKCWIPIVNEILAPEPLKNVSFDAYFTHVLMHE
                     VCHGLGPGILKNPDGSETTVRNELKECYSTIEECKADVLGIFSYLYLIDKGVFAEDSK
                     YSALATYLGGMFRSIRFGINEAHGGCVAIQFNYFIDKGAFLTDAEGKLTIAPAKLETA
                     IKDLAAELLLIEAKGDYEGAKAIIEKYRIATPLITETVKKLEHLPIDIRPIYPITF"
     gene            complement(1866..3116)
                     /gene="pepT"
                     /locus_tag="PKX09_07465"
     CDS             complement(1866..3116)
                     /gene="pepT"
                     /locus_tag="PKX09_07465"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOU17695.1"
                     /translation="MDKEILERFFRYVKIDTQSQEDVTDRYPSTAKQLDLAKILVKEL
                     IDLGLKDAAMDEFGIVMATLPSNLAPTEAARIPVIGLIAHLDTSPEVSGKDVKPIIHQ
                     NYRGGDIKLPGDPNVIIRQSENPILAEKIGEDIVTSDGTTLLGADDKAGIAEIMSFLS
                     YLQKHPDIKHGTLRIAFTPDEEVGAGTDHFDVKKFGADFAYTVDGETVGEIEIETFNA
                     SAGIFTVHGINVHPGYAKGKMVNAIPIAAEIVRLIDREPSPATTEKREGYLHPYVIEG
                     GVEKVTLRVLLRDFDADGIRAKTVRLNEIRDQVASQFPRARVELEVKESYKNMYVKLA
                     ENPKVIEYALEATRRAGIQPKTQIIRGGTDGAKLCFMGLPTPNIFTGGHNFHSKQEWI
                     SVQDMEKTVATLVNLVQVWVEKSL"
     gene            3336..4220
                     /locus_tag="PKX09_07470"
     CDS             3336..4220
                     /locus_tag="PKX09_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014641607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOU17696.1"
                     /translation="MNHNRNHNQNTDNNIGLAFGLNLGFAIFELFGGLWVNSIAVISD
                     ALHDFGDSLSLGVAWYFKRLSLRQKDNRFTYGYQRYALLGAIITMIVLVVGSVIILWE
                     AFPRLLHPTIPNSTGMFVFAIVGIVVNGFAVLRLKGYSSLTEKAITWHLMEDVLGWIA
                     VLIVSVILKFTDWYFLDPLISILITIFILYKVIGNFRATMAVFLQSAPANIDNDKIVA
                     HLSQLEKVKSAHHTHIWSLDGEHHVLTTHIVVEADTSKDEVLMIKNRIKELAEKYNFE
                     HLTVEIEYEGEDCSMNCD"
     gene            complement(4240..4770)
                     /locus_tag="PKX09_07475"
     CDS             complement(4240..4770)
                     /locus_tag="PKX09_07475"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOU17697.1"
                     /translation="MPAKPVKYFIGALFSDKNLLAEALRRAENLYDTADLVSADFPFN
                     LTDYYTSEMGSPIFRRFVSFSKLFSPDFLASAKIHTNSIEDDLRLDGLRKVNLDIGYV
                     DYDKVVLASAKYGIHKIYLGQGIYADLTLHYVKGHYQPYDWAFQDFKLPDYHLFFLKM
                     REIYKQQIKQMLLACQ"
     gene            4936..5778
                     /locus_tag="PKX09_07480"
     CDS             4936..5778
                     /locus_tag="PKX09_07480"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOU17698.1"
                     /translation="MAKKTSVFKIVLFLICVVIILIYGFNRLKDRLPRKLINVIAPTK
                     ITVMTYNINHGMGNDNVYNLSRISKVIKNESPHIVILNEVDYKTRRGLNDEQARRIAA
                     ELGMEFTFSRNYPVDGGWTGNAILSKFPIEFAENKIYKQDYGHNTKSLLHIIVNIGNI
                     KVHVYGTQLSSDSIASSNQVKELLSFVFEWGNDSPVIIGGNFEMTPDYKRIYEMAYYF
                     TDVGAYFQTEAFTYPASEPYKRLDYLFSNKLAIPLKVKVSDNEITRVASDHLPVVAHF
                     KIIY"
     gene            5893..6609
                     /gene="rsmI"
                     /locus_tag="PKX09_07485"
     CDS             5893..6609
                     /gene="rsmI"
                     /locus_tag="PKX09_07485"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOU17699.1"
                     /translation="MKSGILYLVATPIGNLADITFRAIETLREVDLIAAEDTRHAAIL
                     LRHYEIRKPVVSYYEENEKRRSQELLDKLSAGQNIALITDAGTPLLSDPGFQLVRRAI
                     ENDIQIVPIPGASSILTALMASGLPTDRFTFEGFLPRKKGRMTRLNELANEPRTMVFF
                     ESPHRVLETLQDFLEKFGDRPAVCAREMTKVYEEFRRDRLSNLIKHFENHPAKGEFVI
                     IVEGIKRKSKNHSGSQPQTH"
     gene            6614..7813
                     /locus_tag="PKX09_07490"
     CDS             6614..7813
                     /locus_tag="PKX09_07490"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOU17700.1"
                     /translation="MTQNVSQPQPLVKSQVALVRCDSYEPARVAAAIRRGIDLLGGIE
                     KFAQSGERIVLKPNLLSAHPPEENVTTHPTVIKAIAEIMLENGVKVFIGDSATRGHLA
                     KILRESGMQTVIDELSLPVADFENGKLVYSPDSVRNKKFTIARGVWESDGIFNVPKLK
                     THHLTRITAAIKNLFGCVPGFLKPAMHVALPAVDDFCRMLVDLSIFLKPRLTILDAIE
                     AMEGNGPSSGNAYPLKLLAFSSDLVAIDSLVCRMMAIKPEYVRTNYWGAQAGLGKINL
                     EEIEILGNGLDTFSAPDFKVQRQPDLRFNQAWQYRHLKGLVTNRPVIDAQKCKKCGDC
                     IDQCPQQPKALSWNGKMKTQPPQFDYKLCIRCFCCQEVCPHKAIDSYTPLLRRIVDKV
                     YTNIYGA"
     gene            7817..8185
                     /locus_tag="PKX09_07495"
     CDS             7817..8185
                     /locus_tag="PKX09_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17701.1"
                     /translation="MIWFYFCVAVLLVLSGTIIFFKAAWFKSLLQFVIQKDLLLIPGV
                     FEIAIGLGTLYFRDLTHMTWFVYLAGLLMFLDGILSVLMSRRLREMYEWFINIEDRKM
                     RSYAIFLFLVALGYFLAAIR"
     gene            8196..9065
                     /locus_tag="PKX09_07500"
     CDS             8196..9065
                     /locus_tag="PKX09_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17702.1"
                     /translation="MSKKSISIIAAIVILVVLLLVNWKNHQTRLAPTSDNGEWFFLQK
                     SDLRKSPHHTKHARIFGKYVRGYNYYILKGIDKVQAHAPDGGEYFTTLKSRQLESPIG
                     YELKIFGKSLIGPPRRSSYSSGATYGAFIEAMNIMYGKGGHDSLDFEHYEALRMQEIG
                     GGKRREGVKFWGYWNAHGFGNHFALVQYSGIGKAVEPRSARPGDFVNIVWKKDGATSA
                     IFLGWFKDKDEKEKIVYWSSQKETNGFGDQIMPVDNIKYLKFVRVTNPEKLFDFNIDM
                     PVNFKVAGDKVVF"
     gene            complement(9145..10965)
                     /locus_tag="PKX09_07505"
     CDS             complement(9145..10965)
                     /locus_tag="PKX09_07505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU17703.1"
                     /translation="MQKMRNHTHVILWALLILFVASLTVGGLVGGADLLDVFSQKSRL
                     KDAAGIVDGEKLDAVRFSELIQNEINNYRDQNQELTESDIEQISDYIWNSYVNETLIN
                     KQIDRYKLYATDNEIYEVLVNNPPQFLIQNEAFQTNGQFDYQKYLNALNNPQGNEWLA
                     VEEYVRIYLPFEKMQYLIESLAMVSDAEVIEEIVLNQTKANFEALVIPYTLVARDTFA
                     IDKSEIKKYYDANRKNFYVPETRSLDFVVFETKPTPADTFSALQTIESIRERLANGED
                     FATLASEFTEDPSGVANGGDLGWFGKGQMVPEFEQAAFALRKGEVSQPVITSFGVHLI
                     KVEDKRVENGRPEVKARHILIKIKTSPETLENIRAQANLFAFDANEYGFQAAADSHKL
                     VVRNTGPFNKDAKYINYFGPFPAAIRWAYSNIPVGTVSEVLTHEMGITVMCLKEINKE
                     HYRPLSEVENQIKNILTSEKRIEKLKSISEEIYALVTQDNKFDNAIAKYPQCLLDSYT
                     DHPVNAPLKNVQHSNAITGTLLALQPGQISKPVLVANRNLVIIKLHSRQDVTPEIIAQ
                     EKEATRMRILNNKKSAFYTAWINDLKANAKIVDNRSNLYY"
     gene            complement(11291..11878)
                     /gene="cysE"
                     /locus_tag="PKX09_07510"
     CDS             complement(11291..11878)
                     /gene="cysE"
                     /locus_tag="PKX09_07510"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOU17704.1"
                     /translation="MNFYFIEDLRAIWRNDPAAKNMLEILTCYTTLHAIMLYRIAHFL
                     HTYLHIPILPRFISVLARFWSGVEIHPGAKIGKGFFIDHGTGVVIGETAEIGDNCVLF
                     HGVTLGGTGHHKGKRHPTIGNNVLIGAGATLLGPIFVGDNAKIGATTVIINRDVPPNC
                     TVVGTPGHIVKRNGKCVNEPLPLARYKHNDTKCNP"
     gene            complement(11880..12800)
                     /gene="cysK"
                     /locus_tag="PKX09_07515"
     CDS             complement(11880..12800)
                     /gene="cysK"
                     /locus_tag="PKX09_07515"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOU17705.1"
                     /translation="MAIHPNILTTIGKTPLVRINKLTEGTSAEILAKIEFFNPGASVK
                     DRIALNMVEDAEQRGILQKGMTIIEPTSGNTGIGLAMVAAVKGYQVVFVMPETMSIER
                     RKILKQFGAQIILTPGNQGMSGAVTEAERLAQQDGYFMPGQFQNPANPEIHRKTTARE
                     IIADLGNRAPDYFVAGVGSGGTITGVGEVLKQVYPDIQIIAVEPTGSPVLSGGKAGAH
                     KIQGIGAGFIPEILNRDIINEIILVEDESAILTARRLATEEGILVGISAGANLFAALQ
                     IARRNRHDCLIIVVFPDTGERYLSTELFEE"
     gene            complement(12922..13665)
                     /locus_tag="PKX09_07520"
     CDS             complement(12922..13665)
                     /locus_tag="PKX09_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOU17706.1"
                     /translation="MNKTELIRTDEYIIHSYEVDCERRLRLAVLNRYMQESAWQHAEM
                     LGAGYASLLSKNFAWVLTRTVISIEELPIWGNSLILSTWPSGLDHLFAYRDFLFLNTD
                     NQEIGRATTSWCVIDLETRRPQPVAEIFPFQLPQDKGLLFAERPPKVESLTGADYVWH
                     TRVGYQDIDTNEHVNNGRYIDWIYETFPLEFLKTHCPQRLEINYLAEAFYNHEIIVTT
                     QKTSEIIYLHSVVKMPDHTELCRAKTIWR"
     gene            13828..14676
                     /locus_tag="PKX09_07525"
     CDS             13828..14676
                     /locus_tag="PKX09_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU17707.1"
                     /translation="MNYWKTIIISLVLFFNVINVLGDEPAATVIPDWSLKQVFKVPES
                     ALYDPERDVIYVSNINGSPSAADGNGFISRVSPNGKILDLHWIEGLNAPKGMAIYRDT
                     LYVADLNAIARIDLKQGRLIGKIDLKGKFLNDITVDENGTIYCSDNVADIIYIVKNGQ
                     PAIWLSGGNYNPNGLLAEKNQLVMLSSSNGTLNYIDYENKSVRKVATIGGSLDGVVAC
                     DNNYLVSSFPGEIYLVSPDGRSQKILDTKTNKISSADIGYIPQKRLLLVPTFSDNSLM
                     TYKIVR"
     gene            14719..>15067
                     /locus_tag="PKX09_07530"
     CDS             14719..>15067
                     /locus_tag="PKX09_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17708.1"
                     /translation="MKIRTFIIFLTIVLLAYFIVNYYIFIHGLKAFGTNQAAKLIYTI
                     LFVLLVSAYFLGRIIERISNGVVSQALEWLGAYWFGAMVYFLLIVIIIDIFHVINHFI
                     PLIPNDLRQYLQQL"
     assembly_gap    15068..15167
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15168..15671
                     /locus_tag="PKX09_07535"
     CDS             <15168..15671
                     /locus_tag="PKX09_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU17709.1"
                     /translation="KFTVPGNHEFIGGIEKATQYLENHGIKVLRDEALQLANGVWIIG
                     RDDYDSKRYSSYVQRLTLKYLIDKINPEQPIIVLDHQPLNLSESIASRIDLQLSGHTH
                     NGQLWPLNYLTANVYQVSHGYKKIDGTHFIVSSGFGTWGPPIRLASRSEVYVIKLRFK
                     PDFGDEQ"
     gene            complement(15741..16469)
                     /locus_tag="PKX09_07540"
     CDS             complement(15741..16469)
                     /locus_tag="PKX09_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOU17710.1"
                     /translation="MKALILAAGLGKRLRPLTNQKPKALIEVGGRPLLQIAIEYLQMY
                     GFDEIIINVHHYADQIIKYLEVNKYLGQNIIISDETDQLLDTGGALKKAAWFFEDSQP
                     FLVYNVDILCDLDLRQFCKYHRESGALATLAVRQRSSERYLLFDEANVLCGWQNLKTG
                     EIIQTRLSDSPLCQLAFSGISVLSPEILRYLPHKNIFSLIDLYLRLAPEFIIKGFQHN
                     ESRWLDVGNPENLAKAAEFFTITS"
     gene            complement(16564..18030)
                     /locus_tag="PKX09_07545"
     CDS             complement(16564..18030)
                     /locus_tag="PKX09_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOU17711.1"
                     /translation="MNPEQEKSLIHLFENWADEKAQNIYQLPSSGSYREYYRIQGNKQ
                     TALGVFNRDHKETRAFIEFAHHFRSIGLNVPRIYADDAKNNIYLIEDLGDLTLYSYLV
                     NNYKNGIFPDKLIDMYKKVIAYLPRFQILADKGLNYSFCYPRSKFDKRSMLWDLNYFK
                     YYFLKLAKVSFNEEELENDFQTFCDFLLQADCDYFLYRDFQSRNILLVDGEPYFIDFQ
                     GGRKGALQYDIASLLYDAKADIPQHLRDEFLDEYIRQVSRYLTIDEKSFREFYWGYVL
                     IRIMQAMGSYGFRGFYEKKEHFLKSIPYAVANLRFILNNVTLPIEIPTLWQALTQIIE
                     SSKYSNNSVVTSVTSSVLKVQINSFSYRNGIPADPSGNGGGFVFDCRGIPNPGRYEKY
                     KSLNGHHPEVIKFLKEETDIDEFLDYVYAIVDKTISNYIERQFTNLMVSFGCTGGQHR
                     SVYCAEELARHLRDKFRIQIELNHTALENTSGLSEIHS"
     gene            18178..>18263
                     /locus_tag="PKX09_07550"
     CDS             18178..>18263
                     /locus_tag="PKX09_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HOU17712.1"
                     /translation="MSKIRIDLRSDTVTKPSPEMRAAMASAEV"
BASE COUNT         5148 a         3572 c         3998 g         5445 t
ORIGIN      
        1 attacaatgt attaaacaac caaacatctt tatttttatg aattattcag gctaactatc
       61 gattgagttt aaatcgaatc taagattaaa ttttgacctt tcgtcgaggt gcttatttaa
      121 tttaaatagt tagaacgtta tcggataaat cggacgaatg tcgatcggta gatgctccag
      181 tttcttgacg gtttcggtga ttagcggagt tgcaatacga tatttttcga taatggcttt
      241 ggcgccttcg taatcgcctt tggcttcgat taaaagtaat tccgccgcaa ggtctttaat
      301 tgcggtttcg agtttagcag gggcgatagt cagcttgcct tctgcatcgg tcaagaatgc
      361 acctttatca ataaagtaat tgaattggat agctacacat ccgccatggg cttcattgat
      421 gccgaaccgt attgaacgaa acattccgcc gagataagtt gccagagcgg agtattttga
      481 atcttcagcg aaaacgcctt tatcaattaa atacaaatac gaaaaaatgc ccagcacatc
      541 ggctttgcat tcctcaatag tggagtaaca ttcttttaat tcattgcgca cggtggtttc
      601 actgccgtcg ggatttttca ggattcccgg cccgagacca tggcagactt cgtgcataag
      661 cacgtgcgta aaataggcgt cgaatgagac gtttttaagt ggttcgggcg ctaaaatttc
      721 attgacaatc ggaatccagc atttttcgta tttggcttcg gctacatttt tcaacattac
      781 tttcttggag cctttggcgg aacgcacccg ctcgtcgttt ggcaaattaa aagccgttgt
      841 ttgaactccg gctttggtgt caccagctga aaaaatttcc tgtacgactt taataggcga
      901 tactttgccg cgactgtaat tgcgatattc tttggcgagt ggcaggttgg cttcgatttc
      961 atcgagatat ttggttactt tggctaattt ttcgctttcg gcgtggtcaa ccatacaaat
     1021 gaaggcttcg taagcggctt tgtaattgaa aagattatcc tcgtacactt catacggtcc
     1081 gatgacgatt tccaggtcgc cggccaggtc catccaatcc atatcgctct ggaaataatc
     1141 gtcggtttta agatcatctg cccgttggtt gagatacttt tttaaagtcg ggtcttgagt
     1201 gatttcggcg gcttccgtca acaaattaga aattgcattg atatatggtt ggtattcttg
     1261 atgataagga attgcgacca gttgaccttt ttggcgcttg atgatggtaa attcactttc
     1321 aaaagccttt ttctgttcag gatgctcggc gatgaatttc tcgaattctt ccctggtcat
     1381 atcttccgga taaaaaccgg cgccgagcgg tttttccttt ttgccgataa acggtttatt
     1441 gtgatcgatt cggttccaag gcccaaacat aatcgtgaat agttcgagat aaggcaaatc
     1501 gttctcatcg gtcgaagtct gtaatttggc caggatttcc gggttttcgg gattgacctg
     1561 gatgagaaac aactggtcaa tgatgcgtcc ggtttcgacc aggcgcacca gaacttgccg
     1621 atcggcaacc gagagtgacg ataggtcaca tttcagttcc acaggcgcaa gtttattaag
     1681 ttcagctttt acataagcca gatcggcttt gctattatcc actttatcac cacacgttac
     1741 attgcttaaa gtcataaaaa ttcccagcca gatcaggttg ataattgccc atattgggat
     1801 tgatttctta atagattgaa acatttttcc tccattttgt attatctcat gttcaataaa
     1861 ataaattaca gcgatttttc cacccaaacc tggacgagat tcaccaaagt tgcaacggtc
     1921 ttttccatat cttgcacgga aatccattcc tgtttgctat gaaaattatg accgccggta
     1981 aaaatattgg gagtcggaag tcccataaaa cagagcttgg cgccgtcagt gccacctcgg
     2041 ataatctgag ttttgggctg aattccggca cgtcgggtag cttccagagc atattcgatg
     2101 actttgggat tctcggctag ttttacatac atatttttat aggattcctt gacttccagt
     2161 tcaacacgcg cccgtggaaa ttgggaggcg acttgatcgc gaatttcgtt caaacggacg
     2221 gttttggcgc gaatgccatc ggcatcgaag tcgcgtaata aaacgcgtag ggtgactttt
     2281 tcaacgccgc cttcaatgac ataaggatgg agatagccct cgcgtttttc agtagttgcc
     2341 ggactcggtt cacgatcaat taatctaaca atttcagccg caatcggaat ggcattgacc
     2401 attttgcctt tggcgtaacc gggatgaacg ttgataccat gaaccgtgaa aattccggcg
     2461 cttgcgttga aggtttcaat ttcgatttct ccgactgttt caccgtcaac ggtataagca
     2521 aagtcagccc cgaatttctt aacatcgaaa tggtcagtac cggcaccgac ttcttcatcc
     2581 ggagtgaaag cgatccgcaa agtgccgtgt ttgatgtctg gatgcttttg caagtaagac
     2641 aaaaaagaca ttatttcggc aatcccggct ttatcatcgg cgcccaatag agttgtacca
     2701 tcggaagtga caatgtcctc gccgattttt tcggctaaga tagggttctc ggattgacga
     2761 atgatgacgt tagggtcacc tggtagttta atatctccac ctcgataatt ttgatgaata
     2821 atcggtttaa cgtccttacc gctgacttcc ggtgaagtat cgagatgagc gatcagccca
     2881 ataacgggaa tcctggcggc ttcagtcggc gctaaattgg aaggcaaagt tgccataacg
     2941 attccgaatt catccatagc ggcatctttt aatccgaggt caatcaactc tttcactaaa
     3001 attttagcta aatcgagttg ttttgccgta ctcgggtaac ggtcggtgac atcttcctgg
     3061 gattgggtgt ctattttcac gtaacgaaaa aagcgttcga gtatctcttt atccatgaac
     3121 tattcctttc tgtctcctaa tttcactggc tataaaatag taattattga taacaatttg
     3181 tatattaaaa agtgcttttg gaaagaaatt ctgttgatga gaaacaaatg tgttttatca
     3241 atacttcaca acattatata aaaacaaaaa ctcctacatc cgtaatgaaa ccaccaatcg
     3301 tgagcctgtg attcccttaa aaaggaattt ataatatgaa ccataatcgt aatcataacc
     3361 aaaataccga caataacatc ggactggctt ttggtttaaa tctcggattt gcgatttttg
     3421 aattgtttgg tggactgtgg gtgaatagta tcgcggtaat ttccgacgct ttgcatgatt
     3481 ttggcgatag cctatcactg ggcgttgcct ggtacttcaa gcgcctttcg ctccgccaga
     3541 aggataatcg tttcacttac ggttatcaac gttatgctct tttaggagcg attattacta
     3601 tgatagttct ggtggtcggc tcggtgataa ttttatggga ggcttttcca cgccttttgc
     3661 atcccaccat acctaattcg accggtatgt tcgtttttgc catagttgga atagttgtca
     3721 acggttttgc tgttctacgt ctaaaaggtt attcgtcgtt gactgagaaa gcgattacct
     3781 ggcacttgat ggaagatgtg ttgggttgga ttgcggtttt aatcgtgagt gtcatattga
     3841 aatttaccga ttggtatttt ctggatccgc tcatttcgat tctcattaca atttttattc
     3901 tgtataaagt aattggcaat tttcgagcga ctatggctgt ttttctgcaa tcggcgccag
     3961 ccaatattga taatgataaa attgttgcgc acctctcaca gttagagaaa gtcaaatctg
     4021 ctcatcatac tcatatctgg tcgctggacg gcgaacatca cgtcttgacg acgcatattg
     4081 tagtcgaggc agataccagt aaagacgaag tgcttatgat taaaaatcgc atcaaggaac
     4141 tggctgaaaa atataatttt gagcatctta ctgtcgagat cgaatacgaa ggtgaagatt
     4201 gttcgatgaa ctgtgactga gtgataaaat aattgactat tactgacaag ctagaagcat
     4261 ttgcttaatt tgctgtttgt aaatttcgcg cattttcaga aaaaataagt ggtagtccgg
     4321 caatttaaaa tcctgaaaag cccagtcgta aggctgatag tgacctttga cataatgtaa
     4381 agtcaagtca gcatagatac cttgcccgag ataaattttg tgaattccat acttagctga
     4441 agccaatacg actttatcgt aatcaacata accaatgtct aagttgacct tacggagtcc
     4501 atcgaggcgc aaatcgtctt cgatagagtt ggtgtgaatt ttagccgatg ccaggaaatc
     4561 gggactaaat aatttactaa atgaaacgaa ccgacgaaaa atcggactac ccatttccga
     4621 tgtgtaatag tccgtaagat tgaagggaaa atcagcgctg actaaatcgg ctgtgtcgta
     4681 aaggttttcg gctcggcgga gcgcttcagc taggaggttt ttatcggaaa aaagggcgcc
     4741 gatgaaatac ttgaccggtt tagcaggcat tggcttcatt tatgagttaa tctattatca
     4801 attttgaaat tttcaaatca gggagttatt cctaaaacac cccaaaaacg ataaagcaat
     4861 tgccaattaa agaaaaattc gtaaaattcc aatcagtttt ttgaatttta ttattttaaa
     4921 cttaaccaac tttatatggc aaaaaaaaca tctgtcttta aaattgttct tttcttaatc
     4981 tgcgttgtaa ttatcctaat ttatggattt aaccggctta aggatcgttt accacgtaaa
     5041 ttaattaatg tcatcgcgcc gactaaaatt actgtaatga cgtataacat taaccacggt
     5101 atgggaaatg ataatgtcta taatctatcg cgcatttcca aggtcatcaa aaatgaatcg
     5161 ccgcatatag tcatcctcaa tgaggtggat tacaaaacca gacgtggttt aaatgatgaa
     5221 caagcccgac gaatcgccgc tgagctcgga atggagttca ccttttctcg caattatccc
     5281 gttgatggcg gctggaccgg caatgctatt ttatcaaaat ttccgattga atttgctgag
     5341 aataaaattt acaaacagga ttatggccat aataccaaaa gtcttcttca tatcattgtt
     5401 aatatcggaa atattaaggt tcacgtatat ggaacgcagt taagtagtga ttcgattgcc
     5461 agctccaatc aagtcaaaga actgctgagt tttgtttttg aatggggaaa cgatagtccg
     5521 gtcataattg gtggtaattt tgaaatgacg ccggactata agcgtattta cgaaatggct
     5581 tattatttca cggatgtcgg ggcatatttc caaacggagg catttaccta tccggcttcg
     5641 gaaccttaca agcgcctgga ttatcttttt tcgaataaac tggcgatacc gttaaaggta
     5701 aaagtgtcgg acaatgaaat tacgcgggtg gcttccgatc atttgccggt ggtggcgcat
     5761 ttcaaaatca tttactaaca attatcaagg cattttcctt ttattcgaaa tggcataatc
     5821 gggcaaattt gtaacctaaa aattatgaaa ttaattaaaa ttttaggaga atctgaataa
     5881 cataacggaa atatgaaaag cggcatttta tatctggtgg ctacaccgat tggcaatttg
     5941 gctgatatca cctttcgagc gatcgagact ctgcgggaag ttgatttaat tgcggctgaa
     6001 gatacccgcc acgccgctat cttattgcgt cattatgaga ttcggaaacc ggtagtcagc
     6061 tattatgaag aaaatgaaaa acgccgcagt caggaattac tcgataaatt aagcgccggt
     6121 caaaatattg cgttgatcac tgatgccgga acgcctctat tgagcgatcc gggttttcaa
     6181 ctggtaagaa gagcaataga aaacgacatc cagattgttc cgattccggg agcgtcgtca
     6241 atactaacgg ctttgatggc gtccggctta cccaccgatc ggtttacatt tgagggcttt
     6301 ttaccacgta aaaaagggcg gatgacgcgc ttaaacgagt tggcaaatga accacgcaca
     6361 atggtatttt ttgagagtcc ccatcgtgtt ctggaaacgc ttcaggattt tctggaaaaa
     6421 ttcggtgacc gaccggcggt ctgtgctcgt gagatgacca aagtttacga ggaatttcgt
     6481 cgcgaccgcc tgagcaatct aataaaacat ttcgaaaatc atccggcgaa aggcgaattt
     6541 gtaattattg ttgagggcat caaacgtaaa tctaaaaacc actccggttc gcaaccacaa
     6601 acccattgaa attatgacgc agaatgtttc acaacctcaa ccgttagtga agtctcaagt
     6661 ggctctggta agatgtgaca gttatgaacc agcgagggtg gcggcagcta ttcgtcgtgg
     6721 gatagatttg ctgggtggta tcgaaaaatt tgcccagtcc ggcgaaagaa ttgtgcttaa
     6781 accgaatctg ctcagcgctc atcctccgga ggaaaatgtc acgactcatc ccaccgttat
     6841 taaagcgata gccgagatta tgttagaaaa cggggtaaag gtttttattg gtgattcggc
     6901 gacgcgggga cacttggcga aaattttacg ggaaagcggg atgcaaaccg ttattgacga
     6961 attaagtttg ccggttgccg attttgaaaa tggcaagttg gtttattcgc ctgacagcgt
     7021 gcgtaataaa aaatttacta ttgctcgcgg agtgtgggaa tccgatggaa tatttaatgt
     7081 tcccaaacta aagactcatc atttgactcg tattacggcg gcaatcaaaa acctcttcgg
     7141 ctgtgttccg ggttttctca aaccggctat gcacgtggct ttaccggcag tggatgattt
     7201 ttgccgaatg ctggttgatt tgagcatttt tctgaaaccg cgtcttacga ttttggatgc
     7261 tattgaggca atggaaggca atggaccgag tagcggaaat gcgtatccac ttaaactgct
     7321 ggcgttttcg agtgatttgg tggcaattga ttcgttagtg tgccggatga tggcgattaa
     7381 acctgaatac gtccgaacca attattgggg tgctcaagcc gggttgggca agatcaatct
     7441 ggaggaaatt gaaattctcg gtaatggtct ggatactttc tcagctccgg attttaaggt
     7501 tcaacgtcaa ccggatttgc gctttaatca agcctggcaa tatcgccact tgaaggggtt
     7561 agtgaccaac cgaccagtga tagatgccca aaaatgtaaa aagtgtggtg attgtattga
     7621 tcaatgtccg caacaaccta aagccttgtc ctggaatgga aaaatgaaaa cgcaaccgcc
     7681 ccaatttgat tataaattgt gcattcgctg tttttgctgt caggaagttt gtccgcataa
     7741 agccattgac agttatacac cgcttttacg acgaattgtg gataaagtat atacaaatat
     7801 ttatggagct tgaataatga tctggtttta tttttgtgtg gcagttctgc tggttctatc
     7861 tgggactatt attttcttta aagccgcctg gttcaaatcc ctgcttcaat tcgtaatcca
     7921 gaaagaccta ttattgattc cgggtgtttt tgagattgct atcggtctcg gaacgcttta
     7981 tttcagggac ttgactcata tgacctggtt tgtatatcta gctggcttgc ttatgtttct
     8041 ggatggtatt ctatcggtgt taatgtcaag acgtttgcgt gaaatgtatg aatggtttat
     8101 caacattgaa gatcgcaaaa tgcgttcgta tgccattttc ctatttttag tggcgcttgg
     8161 ctatttttta gcggcgatta gatgatgagt aaaatttgag taagaaatca atttccatca
     8221 ttgcggcgat tgtaattctg gtagttctgc tactcgtcaa ttggaaaaat catcagaccc
     8281 gactcgctcc gacctctgat aatggtgaat ggtttttttt acaaaaatcg gatttgcgta
     8341 aaagcccgca tcatactaaa catgcccgga ttttcggaaa atatgtgcgc gggtacaatt
     8401 actatattct gaaaggtatt gataaagtcc aggcgcatgc acctgatggg ggagaatatt
     8461 ttactacttt aaaatctcga cagcttgaat caccaatcgg ctatgagcta aaaattttcg
     8521 gtaagtcgct gattgggccg cctcgtcgga gcagttatag tagcggcgct acttacggag
     8581 cttttatcga ggcgatgaat attatgtatg ggaaaggtgg acatgatagc cttgattttg
     8641 aacattatga ggctcttcgt atgcaggaaa taggcggcgg caaaagaaga gaaggcgtca
     8701 aattttgggg ttattggaac gcgcacggtt tcggtaatca ttttgcgctg gttcaatata
     8761 gcggtattgg aaaagcagtt gaacctcgga gtgccagacc aggcgatttc gtcaatattg
     8821 tttggaaaaa agacggtgca acatcagcca tttttctcgg atggttcaag gataaagacg
     8881 aaaaggaaaa gatcgtctat tggtcaagtc aaaaggaaac caacggcttc ggcgatcaaa
     8941 taatgccggt tgacaatatc aagtacctta aattcgtacg agtgaccaat ccggaaaaat
     9001 tatttgattt taatattgat atgccggtca atttcaaagt ggcgggtgat aaagtcgtat
     9061 tttgatggag aaattgtttt aatattttga ataaaaaagc ccggtagatc gccgggcttt
     9121 tacttttatg atataattga atttttaata atacagatta ctgcgattat ctacgatttt
     9181 ggcgttggct tttaaatcat tgatccatgc cgtgtaaaat gccgactttt tattgtttaa
     9241 aattcgcatc cgggtggctt ctttttcctg ggcgataatt tccggagtaa cgtcctgacg
     9301 ggaatggagt ttgatgatta ccagattacg attagcgacc aacaccggtt tagatatttg
     9361 cccaggctgt aaagctaata acgtaccggt aatagcattg gagtgctgga cgtttttcag
     9421 tggtgcattg acgggatgat cggtatagga atcgagcagg cactgaggat atttggcgat
     9481 ggcattgtca aatttattat cctgagttac taaagcataa atttcttcgc tgatagattt
     9541 taatttctcg attctttttt ccgaggttag gatattttta atttgatttt caacctctga
     9601 caaaggacga tagtgttctt tattgatttc tttaaggcac ataaccgtga tgcccatttc
     9661 atgagtcagg acttccgata cagtgccgac tggtatattg gaataagccc atctgatggc
     9721 ggcgggaaaa ggaccgaagt aatttatata tttggcatcc ttgttaaatg gtccagtatt
     9781 ccgaacaact aatttatgtg aatcagccgc ggcttggaaa ccgtattcat tggcatcgaa
     9841 ggcaaataga ttagcctggg cacgaatatt ctctaaggtt tccgggctag ttttgatttt
     9901 gattaaaatg tgacgggctt tgacttctgg tcggccgttc tcgactcttt tatcttcgac
     9961 tttaatcaaa tgaactccga aagatgtgat aaccggctgg gatacttcac ctttacgtaa
    10021 agcaaaagcg gcttgctcga attcgggtac catctgacct ttgccgaacc aacccagatc
    10081 gccaccgtta gcaacaccgc tcgggtcttc ggtgaattct gaagccaaag tggcaaaatc
    10141 ttcgccgtta gccaagcgtt cccgaataga ttcgattgtc tgtaaagcgg agaaagtatc
    10201 cgccggagtg ggcttggtct caaagactac aaaatccaaa gagcgagttt ctggaacgta
    10261 aaaattcttg cggttggcat cataatattt ttttatttct gatttatcaa ttgcaaaagt
    10321 atcccgagcg accaaagtgt aaggtattac cagcgcttcg aaattggctt tagtctgatt
    10381 taacacgatt tcttcaatga cttcggcatc cgaaaccatc gctaaacttt cgatcaaata
    10441 ctgcattttt tcgaaaggca agtaaatacg aacatattcc tcaacagcca accattcatt
    10501 gccctgcggg ttgttaaggg catttagata tttctggtaa tcgaattggc cattggtttg
    10561 aaacgcttca ttttgtatca aaaactgagg tggattatta accagaactt cataaatctc
    10621 attatcggtc gcgtagagtt tgtagcgatc gatctgttta ttaatcaacg tctcgttgac
    10681 gtatgaattc cagatataat cggagatctg ttcaatatcg gattctgtca attcttgatt
    10741 ttggtcacgg taattattga tttcattttg aattaattcc gaaaagcgca ctgcgtccaa
    10801 tttctcgcca tcgacaattc cagcagcatc tttgagacgg gatttctggg aaaagacatc
    10861 cagtaaatcg gcacctccaa ccagtccgcc gactgtcaaa ctggctacaa ataggattaa
    10921 aagcgcccag agaattacat gggtgtgatt ccgcattttc tgcattagag ccatagttta
    10981 tcaagacctc cgaattttat attaaccact tcaaaaattg acgctaaata taagtaaaat
    11041 tgccgggatt gacaaaaagt tatataagca ggacttcact caaattttta ggaaaataga
    11101 taaggctttt taagtctatg aattatgctg ttatttagct agttatcccg tctgtatcct
    11161 aatttttggg gatgtcttag gtattttgag taagtatcta tgaaatgaac aatcctgatg
    11221 ctcacctcgg ggacaccgga attaaaattg tcggtttcag cgagaaaatc cctccgcttt
    11281 tcttcatatt ttatggattg cacttcgtat cattgtgttt ataacgtgcc agcggaagtg
    11341 gttcattaac gcacttgccg ttgcgcttga caatatgacc aggagtaccg acaactgtgc
    11401 agttaggtgg aacatcacgg ttaatgataa cggtcgtggc gccaattttg gcattgtctc
    11461 ccacaaaaat cggcccgaga agagttgcac cggcgccgat aaggacgtta ttaccgattg
    11521 taggatggcg tttgcctttg tgatgaccag tgccgccgag agtaacacca tgaaacaaaa
    11581 cgcaattatc accgatttcg gcggtttcgc cgatgacaac tcctgtacca tgatcaatga
    11641 agaatccttt cccgattttt gcgccgggat gaatttcaac tccgctccag aagcgagcta
    11701 atacactgat gaagcgtggc agaatgggaa tgtgtaaata ggtatgcaaa aaatgcgcaa
    11761 tccgatagag cataattgcg tgtagagtag tatagcaagt taaaatttca agcatatttt
    11821 tggcggctgg atcgttgcgc caaatggcgc gaaggtcttc gataaaataa aaattcattc
    11881 tattcctcaa acaattctgt gctcaaataa cgttcgcccg tgtctgggaa aaccacaata
    11941 atcagacaat cgtgccgatt acgccgggca atttgaagag cggcgaacag gttggcgccc
    12001 gctgaaatac ccaccagaat accttcttcg gttgccaaac ggcgggcagt caaaatagcc
    12061 gattcatcct caaccaggat aatttcgttg atgatgtcac ggttcagtat ttcgggaatg
    12121 aatccggcgc caataccttg aattttatgc gctccagctt taccaccgga taacaccggg
    12181 gaaccggttg gttcgacggc aataatttga atatccggat agacttgctt aaggacttcg
    12241 ccgacgccgg ttatcgtgcc gccactaccg acaccggcta cgaagtaatc tggagcgcga
    12301 ttgccaagat cggcaataat ttcgcgagcc gtagttttac ggtgaatttc cggattagcg
    12361 ggattctgga attgtcccgg cataaagtaa ccatcttgtt gcgcaagacg ttcggcttcg
    12421 gtgactgcgc ccgacattcc ttgattgcca ggtgttagga taatctgtgc tccaaattgc
    12481 ttcaatattt tgcgacgctc gatgctcatc gtttccggca taacaaatac gacctgatat
    12541 cctttgactg cggcgaccat cgccaagcct attccggtgt taccggaagt tggttcgatg
    12601 attgtcatcc ctttttgtaa aattccgcgc tgttcggcat cttcgaccat attcagagca
    12661 atacgatctt tgactgaagc gcccggattg aaaaattcaa tcttagccaa gatttcagcc
    12721 gatgtccctt ctgtcaattt attaattcgt accagaggag ttttaccaat agttgtcaat
    12781 atatttggat gtatagccat cacttttcct aattttcgta aactttaagg ctctcgtaat
    12841 ttaaatccaa aggaatttta gaggggaatt aaaaattcca gtaaaatttg tctgaagcat
    12901 cggcatatat tttaattcaa tctaccgcca aatcgttttg gcgcggcaaa gttcggtatg
    12961 gtcgggcatt ttaacaaccg aatggaggta gattatttcc gacgttttct gggttgtaac
    13021 aataatttcg tgattgtaaa aagcttcagc taaatagttg atttccagac gttgagggca
    13081 atgagtttta agaaattcga gaggaaaagt ctcataaatc cagtcaatgt agcgcccgtt
    13141 gttaacatgt tcgttagtat caatgtcttg atagccgact cgggtatgcc agacataatc
    13201 cgcaccggtg agcgattcga cttttggtgg acgttccgca aaaagtaacc ccttatcttg
    13261 cggcaattga aaaggaaaaa tctcagccac tggttgaggg cgacgagttt ctaaatctat
    13321 cacgcaccac gaagtcgttg cccgaccgat ttcctgattg tcagtgttta ggaataagaa
    13381 gtcacgataa gcgaagagat gatccaatcc ggaaggccag gtcgaaagta tcaaagaatt
    13441 cccccaaatc ggtaattctt cgatactaat gacagtccga gttaacaccc aggcgaaatt
    13501 tttcgataac agactggcat atccggcgcc cagcatctcc gcatgttgcc aggctgattc
    13561 ctgcatataa cgattcagca ccgcaagtcg caggcgtcgc tcgcaatcaa cttcgtagga
    13621 atgaataata tactcgtcag ttctgattag ttcggtctta ttcataattt tttcaaacga
    13681 tagataatat aacaaacttc agtgaaaata aattctgaca aaacatttgt acctgttaat
    13741 tttctgcaat taccctatta aaaaattgtt gatttgttgc tcagggattt ttaagattaa
    13801 atcaatgaag aaaatggagg atagattatg aattattgga aaacaattat catttctttg
    13861 gtgctttttt tcaatgtaat taacgtcttg ggtgacgaac cggctgctac cgttattcca
    13921 gactggagtt tgaaacaggt atttaaagta ccggaatcag cgctgtacga tcctgaacga
    13981 gacgtcattt atgtcagtaa tattaacggc agtccgtcgg cggcagacgg taacggtttc
    14041 atttcgcgag tatcgccgaa tggtaaaatt ctggatttgc actggattga aggtttgaat
    14101 gccccgaaag gtatggcgat ttatcgagac acgctttatg tcgctgactt gaatgcaatt
    14161 gcacggattg atctaaagca ggggcggctc atcggtaaaa ttgacttgaa aggcaaattt
    14221 ctaaacgaca ttaccgttga cgagaacggt acaatctatt gttcggacaa tgtcgctgat
    14281 attatttata tcgtgaaaaa tggacaacca gcaatctggc tctccggcgg caactacaat
    14341 ccaaacggtt tactggctga gaaaaaccaa cttgtgatgc tttcttcaag caatggcaca
    14401 ttaaattaca ttgattatga aaacaaatct gttcgtaaag tggctacgat tggcggctct
    14461 ctggatggtg tagtagcctg tgataataat taccttgtat ctagtttccc gggagagatt
    14521 tatttggtaa gtccggacgg ccggagccaa aaaatcctgg atactaaaac taataaaatc
    14581 tcatcggctg atattggata cattccccag aaacgcttac tattagtacc cactttctca
    14641 gataatagcc tgatgacgta taaaattgtt cgttagattt aatccgttta ataataagca
    14701 atcaggttta gattttacgt gaaaattcgg acgtttatca tattcctcac gattgttcta
    14761 ttagcttatt ttattgtcaa ttactacata tttatacacg gacttaaagc ctttggtaca
    14821 aaccaggcgg caaagttgat ttatacgatt ttgttcgttc ttctggtttc ggcctatttt
    14881 ctcgggcgga ttatcgagag aatttccaac ggagttgtct cgcaagcgtt ggaatggctc
    14941 ggagcttact ggttcggcgc tatggtttat tttttactaa ttgttataat aatcgatatc
    15001 ttccatgtca ttaatcactt tattccgctg attccgaatg acttacggca atatcttcag
    15061 cagttgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa tttaccgttc
    15181 ccggcaatca cgaatttatc ggcgggattg aaaaagctac ccaatatctt gaaaatcacg
    15241 ggataaaggt cttacgtgat gaagcgttgc agttggctaa tggcgtctgg ataatcggtc
    15301 gggacgatta cgatagcaaa cgctatagta gttatgttca gcgacttaca ttgaaatact
    15361 tgattgacaa aattaatcct gaacagccga tcattgttct tgatcatcaa ccgttaaatt
    15421 tatccgaaag tatagcaagt cgaatagatt tgcaattatc tggacatacc cataatggac
    15481 aattgtggcc attaaattac cttaccgcaa atgtttatca agttagtcat ggttataaaa
    15541 aaattgatgg cacccatttt atagtatcaa gcggttttgg aacgtgggga ccgccgattc
    15601 gccttgccag tcgttcggaa gtatatgtaa taaaattgcg ctttaagccg gattttggtg
    15661 acgaacaata aaatttatgg tgtttgcgta ggacagacat tcctgcctgt agattttaat
    15721 gacagcctgg aaaggctgtc ctacgaggtt atagtgaaaa attctgcagc tttcgccaga
    15781 ttttcgggat taccgacgtc cagccagcgg gattcattat gctgaaaacc cttaatgatg
    15841 aattctggcg ccaaacggag atagaggtct atcagcgaaa aaatattttt gtggggtaaa
    15901 tagcggagaa tttccggact gagcaccgag attccgctga aagctaattg acacagaggt
    15961 gaatcggaga gacgcgtctg gataatttcg ccggttttga ggttttgcca accacatagg
    16021 acattagctt catcgaaaag taaataacgt tccgacgaac gctggcgaac cgccaaggtg
    16081 gctaacgcgc ccgattcgcg gtgatactta caaaattgcc tcaggtcgag atcgcacaaa
    16141 atatcaacat tataaaccag aaaaggttgg ctatcctcga aaaaccaggc ggcttttttt
    16201 aaagcgcctc ccgtgtcgag aagttgatca gtttcatctg aaattataat attttgcccg
    16261 agatatttat tgacctcaag atattttatg atttgatcgg cataatgatg aacattgata
    16321 ataatttcat caaagccata catttgtaaa tattcgattg ctatttgcaa taggggacgg
    16381 ccgccaactt cgatcaatgc tttaggtttt tggttggtga gcggtcggag ccgtttaccg
    16441 agtccggcgg caagtataag agctttcata ttggtgatcg caaattgtta acggacggta
    16501 attttagggc aggcttgaaa ctaaaaacca taaaatgaga aataggagtt ttacctaaaa
    16561 ttttcatgaa tgaatttctg aaagtcctga tgtattttcc agcgcggtgt gatttagttc
    16621 gatctggatt cggaatttat cacgtaaatg acgagcgagt tcttcggcac aataaaccga
    16681 gcgatgctga ccgccagtac agccgaaact taccatcaga ttagtaaatt gccgttcgat
    16741 gtaattactt atagttttat caacaatggc atagacgtaa tccaggaatt cgtcaatgtc
    16801 ggtctcttct tttaagaatt taatgacttc tggatgatga ccattgagag atttgtattt
    16861 ttcgtagcgg ccgggatttg gaatgccacg acaatcgaag acaaaaccac cgccgtttcc
    16921 actcggatcg gctgggatgc cgttccggta ggaaaagcta tttatctgga cttttaagac
    16981 acttgaagtg actgaagtta ccaccgaatt gttagaatat ttcgatgatt ctataatctg
    17041 agttagtgct tgccaaaggg ttgggatttc aattggcaga gtcacgttat tcaaaataaa
    17101 acgcagatta gccacggcat aggggatgct tttcagaaaa tgttcttttt tttcgtagaa
    17161 tccgcgaaat ccgtaggaac ccatcgcctg cataatcctg atcaaaacat agccccagta
    17221 gaattcacgg aacgattttt cgtcaatcgt aaggtagcga ctgacctgcc gaatatattc
    17281 atccagaaat tcatcacgca aatgttgggg gatatcggct tttgcatcat agagtaaaga
    17341 ggcaatgtca tactgtaagg cgcctttcct accgccctga aaatcaatga aatatggttc
    17401 gccatcaaca agcaaaatat tgcgcgactg aaaatcgcga tagaggaaat aatcgcaatc
    17461 agcctgtaga agaaagtcgc aaaaggtctg gaaatcgttt tcaagctcct cttcattaaa
    17521 gctgaccttt gccagtttca gaaaataata tttgaaataa ttcaaatccc ataacattga
    17581 gcgcttatcg aatttcgaac gcggataaca aaaggagtaa ttcaaaccct tgtcagccag
    17641 aatttgaaaa cgcggcaaat acgcgatgac ttttttatac atatcaatca gtttatcggg
    17701 gaaaatgcca tttttataat tattaacaag gtaggaatat aaggtcaaat cgccaaggtc
    17761 ttcgataagg tagatgttat ttttcgcatc atcggcataa attctcggta catttaatcc
    17821 aattgagcgg aaatggtgcg caaattcaat gaacgcccga gtttccttat gatcgcgatt
    17881 gaaaactccc aaggcggttt gcttgtttcc ttgtatccga tagtattcgc ggtatgaacc
    17941 ggaactgggt aattgataaa tgttttgggc tttttcgtca gcccagttct cgaaaagatg
    18001 gattagtgat ttttcttgtt cggggttcat agctataatt tcggatgttc gtagtcatta
    18061 tggaaggatt tttctggatt gggaaaatta tttcactaac catcatcccg aatttgaaaa
    18121 accgctaata attaattaat ttgaaacggt aaaatttata aaggaaatgt taacggtatg
    18181 agtaaaattc gcatagattt acggagcgat acagttacca aaccttctcc tgaaatgcgg
    18241 gcggcgatgg cgagcgccga agt
//
LOCUS       DAOSMP010000041        16910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_3415_length_16910_cov_22.556274, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000041 DAOSMP010000000
VERSION     DAOSMP010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 16910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16910
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3415_length_16910_cov_22.556274"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(404..793)
                     /locus_tag="PKX09_07555"
     CDS             complement(404..793)
                     /locus_tag="PKX09_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17713.1"
                     /translation="MRRLIQNVIGFLQRNYKLLITWAIIIIAVIVGIHFRVDKGIIVV
                     LVMIFGLFAEAFSGLIALLATVPVIGPIVAKVLTLPIFWIFNALGYFVSLVAIKRGYK
                     KEVINYRFVTMVFLTGFAVGFIIAKLL"
     gene            complement(873..1667)
                     /locus_tag="PKX09_07560"
     CDS             complement(873..1667)
                     /locus_tag="PKX09_07560"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOU17714.1"
                     /translation="MLLAIDIGNTNATVGLFQENDLKGCLRINSHPVRGADYYGRRIE
                     SLLKKQGIAINVIDGIVIGSVVPRLTEPFKQMAQTFFHIEPLLINCNLNLGCCLLVDN
                     PAEVGADRICNAVATGVKYHCPSIVVDLGTATTFDVLDQEGNYIGGAIAPGLITGARD
                     LFRKAAQLYPINLTFPPRVIGKNTTAHMQSGILFGHLAMIEGMIRRIGVEINQPDIFV
                     IATGGFSNVIAQHSPLIQASDPYLTLDGLRLIYGMNLPQTSKSQSR"
     gene            complement(1670..2479)
                     /locus_tag="PKX09_07565"
     CDS             complement(1670..2479)
                     /locus_tag="PKX09_07565"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOU17715.1"
                     /translation="MKSLTCAKVMSSFRLVNLPIPTEILRFPVVHFAEIDSTNAYLLD
                     RHSHPSGTIVWADFQSAGRGRLGRSWITPPNEALLFSIYLKIHHNETPLFIYSFLTAV
                     GVLEGLQTLVTPAWLALKWPNDVLLKNMKVCGILVQSKTSSGDNADVVIGIGVNLNQN
                     SDFFIPDLPFAGSLYSITGKKYEIESVLAKLIFAIDQNLVALNGNGTVPILEKWRKYC
                     PYIGQVIKVNDGQEIHSGLFYDISADGGLILQVGRTYRTFHAAEVTIVRET"
     gene            complement(2457..>4120)
                     /locus_tag="PKX09_07570"
     CDS             complement(2457..>4120)
                     /locus_tag="PKX09_07570"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOU17716.1"
                     /translation="GLTGRVVAMLDGASYYLYHYRKFRDIRLVYAPPQSIGNFGGEVD
                     NWMWPRHTGDFSFMRIYCAPDGKPAEYSEKNVPYQPKAFLPISTRGLNPDELVFILGY
                     PGRTYRNVTSYSVAYNQNLVYPLRIRIFQEIINELEDESQKSPEVDLLLSSRLKGFYN
                     GLKNNQGLLAGFKSENILGQKKLVEKELVQKIAGKPAWQEQYGNILPEIQKAYDEYYT
                     GFERDMYIEYLRYVTVLADALTIEKWSREKAKPESEREYGFFDYQIARTKRDFKVRRI
                     GYYAPADARILKLLLNHLAEFSSAERPAFLRELIQDQPADSAKAVIAAYVDSAFAHTR
                     LTDADECLAMFDLSAESLLDRQDPLITLAVAVNCELERIEKDQKTISGKMLILKPQYL
                     SALKNVLGKTVLPDANRSLRFTYGNVEGYQPRDAVFYQPQTTLAGVIAKHSGAEPFDV
                     PEKLSELANSRDYGRWIDRELNDVPVAFLSSCDITGGNSGSPVLNARGELVGCAFDGN
                     WEALTNDWQYNPALSRAISVDVLYVLFILDKYSSADELIEELDLR"
     assembly_gap    4121..4220
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4221..4765)
                     /locus_tag="PKX09_07575"
     CDS             complement(<4221..4765)
                     /locus_tag="PKX09_07575"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOU17717.1"
                     /translation="MSNIKRLFFLATCLLTLNFRLLAAEGMYPPDQIPTKIKGLKVKP
                     ADIYQPDGGGLAGAVLLFGGGTGSFVSADGLVLTNHHVAFGAIQKNSTDEQNYLAQGF
                     YARTKADELPAPGYEASLMIGFEDITEKILKSFTPQMTPEERTRALERAIAQLEAESE
                     DSANGLTGRVVAMLDGASYYL"
     gene            complement(4790..5542)
                     /locus_tag="PKX09_07580"
     CDS             complement(4790..5542)
                     /locus_tag="PKX09_07580"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOU17718.1"
                     /translation="MGKLAGTIIGGGIGWALFGPLGALIGAYIGNVVTNESGVQKKRG
                     KAEPGATFATYGDTRAGDFAVAMLALFAYVSKSDRNVKSSEVQYVKKFLVDKFGTQNA
                     QDLLYLYREILGKDYNIYDIAHQVRDNMDYYSRMELLHVLFGIAAVDGDLHTTELQVI
                     REIADALGISDRDYETIRAIFGGGTNRFYEILGIKPNDDVEIIKKAYRDLAVKYHPDK
                     VANLGPEFQKLAEEKFKSINDAYQTIRKERGF"
     gene            complement(5671..6786)
                     /locus_tag="PKX09_07585"
     CDS             complement(5671..6786)
                     /locus_tag="PKX09_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17719.1"
                     /translation="MRRTLSRIVVLTLFMVATIMAQEFSMDQIKELAATNGKLYLQPL
                     ATGFGTAMNSGLYNTAGTHGTLGFDIQLKTGVGLAPASKASQTTYQFDLSALDDIAFN
                     IEKNSQSYSINLDPEDIFLDCTTPNILGGEAKPFTVNQPYVQEEIESQLRTQLQAQGL
                     PAEQINAAITEMAPEIQEAINGIQVKSIPGMKSIKALEDLKTVPFPFPILQASVGLPM
                     GIEPSIRIIPKFKIPGDIGEAIGEISALGGGLKLDVDRWIPIPMFPLDIAVQGYYQKM
                     EIGDLLSANSTAFNLMVSKTILMLTPYVGVGKESSKFTASYKMEDGTKIKFDLKGKND
                     TRFTAGLNIKIAVISINAEYSMGEYNAAGLGVGFALR"
     gene            complement(6854..>9021)
                     /locus_tag="PKX09_07590"
     CDS             complement(6854..>9021)
                     /locus_tag="PKX09_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOU17720.1"
                     /translation="FPTLNNTTDSLFLYDATGQIIDSVRYRDSWGGGTGVSLERLDPA
                     QPALNAGNWGGSLIIATPGQTNSIIKLDNDLAIVPSSFKFSKTLDAVGQKVSFSLKIK
                     NVGRNSAENFSIKIYLDQNQDSCRQADELCWRQDINQSLKSDSTLIVTGKITAERAGR
                     SLYLVELEFSFDENLDDNYAGTDLVVAFPSGCLTLNEFLAYPTSNQVEFIECINVSDE
                     TIDLSNWMLGNRYLKNEISGGKVPPGGYVILSGDSACFNYFKMDNAAVIIPGKWPGLN
                     NSADQIWLKDLSGKIIDSLYYGAGWPIKAGYSAEKILPHLASDTSQSWALSVSPEHQT
                     AGYFNSVTRPENDLSLDSLQLSAITGDTNTVFAGCYFISNKGQNQACGANLIISLEND
                     LISQIPLDCIGLGQADSGNFEIGPLTSGKHTVTVRVDWPADQINDNNMLLRSIQISWS
                     RGSVLLSEFMPYPWDIRTASSSVAEYLEFYNSSENTIDLAGWMISDQNTAVRRSIPSG
                     YFLSPGEYFVIAGDSSVLNFPDLAPSQTWITAKFPSLNNSEDAVYLYDPTGRAIDSLF
                     YNSTWPIIQGIAMERISFDNSNRAMNWRASVAAAGGTPGQPNSVILPEKLDKLGIKPT
                     ADVFSPDGDGIDDEIGIQYLLPFPSAKVTIEIYDLVGRLIYRLATNLETAASGVIYWN
                     GASDYSPKARVGIYIVRCLANDIASAKSVEYITTLVLAR"
     assembly_gap    9022..9121
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9122..10336)
                     /locus_tag="PKX09_07595"
     CDS             complement(<9122..10336)
                     /locus_tag="PKX09_07595"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOU17721.1"
                     /translation="MNLTKATAAVLFIIDVFITGLDGQAITINEVMADPVGSDYYDEF
                     MELYNYGDLAVTLSGYYLEINGYVDTLSFVADYGDVLLPHSFALIMDRGYLVDYKSSS
                     YEDLIPENTLLVTIQDNSLAHTGLINSGASQIYLFNAQRDTISAVLTTAKPLSGYSWE
                     KIIPEGTNQISNWGNSRNQRGTPGFWNSITPRDYDVAIEGLKMVSPVDQIFPHQPIDL
                     QFIVKNVGIKSADSLQVVCGLDQNSDRQIQIDEIFYDEIITLTAGDSVMQNATIPGLP
                     SGIYTILAEAIYRADERSDNNLESLDIKILFSENCLIISEFMYYPTTDGGGEWVELLN
                     ISADTINLKRLTLSDNSTTIMLTDQDFYLPPQKYLVIVNDSTTFISYWGQVPFILDCP
                     KTMPALNNTKDSI"
     gene            10502..>11769
                     /locus_tag="PKX09_07600"
     CDS             10502..>11769
                     /locus_tag="PKX09_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17722.1"
                     /translation="MKNLIFSKFAFLKKMERFLLMLGLFGTLWSNNLYPQSERYTRKS
                     VAFLDALMVTDSRIRLNPEDEKYLLTAIHNGIRIARFDYNPLPDMVRYTFKEQLRAKG
                     VVSESELIALIEATIVPEIVRILDIEKEIRAQNLVDETQRNSFISIKAKELGITADQI
                     EQVMNASYIYLPFLTSYTVSKPKDDPNLTVTIKGGLFWYHIVPGDDPYLESIVTLHTE
                     ASGSAEKAKSDAESEAFRLAASTFAINLQVLTRDIEMFKLSAPIAAIEKRTVKFPLGT
                     AEGIKLDDPFFVGEYFQTSGDKITFKKSGFVRVSKVGDNRQDPRQLSAAYAIQKGDWV
                     KGMTMVEHPRVGVDVALKPRGFFMNINEGVFASEDFLIFFDDYDGFAFGADLDFQINI
                     APLTNKRQSFLVAGATAGVVPVKSELYSTDT"
     assembly_gap    11770..11869
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11870..12079
                     /locus_tag="PKX09_07605"
     CDS             <11870..12079
                     /locus_tag="PKX09_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17723.1"
                     /translation="IGIFAGFQAFPSFDWWTIKYGDKEIDVANYADTDKPSISSLSPT
                     FGIYFHYSPPTLSFNPASAFQANGN"
     gene            12097..13374
                     /locus_tag="PKX09_07610"
     CDS             12097..13374
                     /locus_tag="PKX09_07610"
                     /inference="COORDINATES: protein motif:HMM:NF040342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6175 family protein"
                     /protein_id="HOU17724.1"
                     /translation="MKKLSVIILTIFCIASLVFAKSEKSKTKSNLPRSHEAVILESTS
                     PSEVMVRATGYSSEGGKAAEMDKEAEADARKAAVWLILLGGTDPLLQTDAEKAEFAKI
                     QEEFFAPSNIKQFIVWESDYYHSRLKIDGGKTLKIEKTFKVNKSLIHDYLVERGILTK
                     TAAITEALGLPTIMVIPETDGKQAPIELLRTNPNLKKGAEVIEAYLTARKFEVIVPEQ
                     QQALQELTSAHYTLQGASEDYSYLLALSIGSDIYITYNVAVESRLLGSSTVKKGVVGC
                     RAYETTTGRLLGTETGYSPERTAPDAVLIEEAMNDAVDKVISRIMAYWKEDIQQGVQY
                     KVILNISDSFTKSEAEDMIYAFGDLLKKASKRIKENVIADYSYDVLIWCDPALYQSSS
                     DLYRFFKQNYKGQGRLERVSLNRKLILLNIVEE"
     gene            13399..14754
                     /locus_tag="PKX09_07615"
     CDS             13399..14754
                     /locus_tag="PKX09_07615"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOU17725.1"
                     /translation="MTKIILVLAGLAITSASAQVPDWYKTNQHKKYPASRYFLGVAVA
                     DTKAEAIELARADVAKQIQVRIESELETIESEFREGDKNYLKAETTNRTKSIVAETVS
                     GIEIAEVKEVKGKTYVLAVLNKEKYLSTLEIQMDEILMKSEQLIASARQMVADGRILN
                     ALNNFIDAQNTIPDFYTKSSLYTALSGRKYPNFNQFTGPGIVAEVREILAHIELKILS
                     GNNQIAVAGQNLPTPIKVQVVYVRDGEIIGVAGFPVIARYDGGEIITKIETDANGIAE
                     FNCVATPTDNVGQSGAVKIALDLSKIPEQYRDAMTRSAVQISYILTANDLKFTVEVIQ
                     KNSQHLTFIEDELVRLIAANGYTCSADAPWLVQATLVIISQKEVPSPAGNQHLVEAQF
                     QLRLIEKQTGNVFAALDINTKGLDSQSPDNALSKAAQNLKIPSNKFAAFLQSANQPKN
                     D"
     gene            14782..15336
                     /locus_tag="PKX09_07620"
     CDS             14782..15336
                     /locus_tag="PKX09_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOU17726.1"
                     /translation="MKKLLVFVLLISVALFTVNCGGKQPKAKMDDIPEWYLNPPQAED
                     AIYQAGDAVKSSMALAKDAADARARDAIARTVELRVTNMMKDFMQQSGMGEDAEALEF
                     TSSVSKQVANTVLKGCRIVKREMKVEGNNYHAYSLAEYNLNSLIEETLNEARRQKALY
                     NEAKANMSFEELEREIEKLKNVGQ"
     gene            15388..16131
                     /locus_tag="PKX09_07625"
     CDS             15388..16131
                     /locus_tag="PKX09_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17727.1"
                     /translation="MKFCLFTIALVLGLKNLAFAQECVPSWFEKLPEVEGIRLAVGYS
                     GKFTNEAGAKSAAVQLAHKNMTKQISIRLQFDIEELADGRIRLLNPSFKEFYEGSILN
                     AVESNSSVIDSTITVEGYFVLLAFPAIEYLPIPNACDKPWNIRPDWIENLPQEDGYVY
                     GIGIVARYRNWVRAWQDADEYSRFDLGKNLQVNTESIHAVQRNNRTTIESVILKQTYD
                     LTINDATIVARWLDTANGVYYSLCRAPRP"
     gene            complement(16243..16407)
                     /locus_tag="PKX09_07630"
     CDS             complement(16243..16407)
                     /locus_tag="PKX09_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17728.1"
                     /translation="MDNYESAITALKIPFFPLGLRFPARDVLNAVSKLNGQILGAPLY
                     SVSLLMTSDQ"
BASE COUNT         4809 a         3709 c         3553 g         4539 t
ORIGIN      
        1 gtttcgcgga aattggtttg gctatttgtg atttggaatt tggagtttgg gatttgagat
       61 ttgtgatttg tgatttggag tttgggattt ttctcattta gcagttcttg gcgaacttgg
      121 cggtttaatc ttccaagacc aagaaaaata aggctgagat tggtcattgg tcattagtaa
      181 agaggttgag aaaagaggct gagaaaagag aatggtcatt agtcattggt cattggtcat
      241 tagtaaagag ggtgagtaaa aagaaagagt aaagaggttg aggttgtggt tgagagagaa
      301 ttcaaatctt agcctttttt ctcagccttg acctctttct tttaggctga ggctgagaaa
      361 atgtagattg agccggtagc acgaccagat gtaaataggt ttactataat agcttggcaa
      421 ttataaaacc gacggcgaat ccggtcagga aaaccattgt cacaaagcga tagttgatga
      481 cctccttttt atagcctctc ttgattgcca ccaaggaaac aaaataacct aacgcattaa
      541 aaatccaaaa aatcggcagg gttaatactt tagccacaat cggtccaatt acaggcacag
      601 ttgccagcag agcaattaag ccggaaaaag cctcggcaaa taatccgaaa atcataacca
      661 gcacaactat aataccttta tcaacccgaa agtggattcc tacaataacg gcaatgatga
      721 ttatcgccca ggtaatcaat aacttgtaat tccgctggag aaagccgatg acattttgta
      781 tcaagcgcct caaaaacaaa agagttcttt tgtcattttg ttgctcgtct tggttcatat
      841 ttttaccata aaattgtttg taaagcctaa agttacctcg actgggattt cgacgtctga
      901 ggtaggttca tcccgtaaat caggcgcaag ccatccagtg ttaaataggg atcgctggct
      961 tggattaagg gagaatgttg agcaataaca ttgctgaatc caccggtagc aattacaaaa
     1021 atgtctggct gattaatctc tactccgata cgacgtatca ttccttcaat catagctaga
     1081 tgaccaaaca aaatcccact ctgcatatga gcggttgtat tcttaccaat aacgcgcggt
     1141 ggaaaagtga gatttattgg atagagctgg gcggcttttc taaaaaggtc cctcgcaccg
     1201 gttataagtc cgggagcaat cgctccgccg atataatttc cttcctgatc cagcacatcg
     1261 aaagtcgttg ccgtgcccag atccacgacg atgctcggac aatgatattt cactccagtg
     1321 gctacggcat tgcaaatgcg atcggcccca acttcagccg gattatcaac taaaagacaa
     1381 catccgagat tcaaattgca attgattagg agcggttcaa tatggaaaaa ggtttgcgcc
     1441 atttgcttaa acggttcggt taggcgcggt acgactgagc caattacaat cccatcaatt
     1501 acattaatgg caatgccttg ctttttcaac aaagattcaa tccgccgacc ataataatct
     1561 gctcctcgaa ccggatgact atttattcgc agacatccct taaggtcatt ttcctgaaat
     1621 agtcccacgg ttgcattggt attacctata tcaattgcta ataacattcc taagtctccc
     1681 tgacgattgt tacctcagcg gcatggaacg tccgatatgt ccgaccgact tgtagaatga
     1741 gaccaccgtc ggcactgata tcatagaaca aaccactgtg aatctcttga ccgtcgttga
     1801 ctttgattac ctgcccgata tacggacaat atttacgcca tttttccaga atcggaacag
     1861 ttccgttacc attcagcgcg accaaatttt gatcaatcgc aaaaatcaat ttggcgagca
     1921 cactctctat ctcatatttt tttccggtaa tagaataaag cgaaccggca aacggcaaat
     1981 cggggataaa aaaatcacta ttttgattca aatttacgcc aattccaatt accacatccg
     2041 cattatcccc tgagctggtt ttactttgca ccaaaatacc acaaactttc atgttcttta
     2101 gtaaaacatc attgggccat ttcagtgcca accaggctgg ggtaactaga gtttgtagtc
     2161 cttccagaac acccaccgca gttagaaatg agtaaataaa aagcggtgtc tcattgtggt
     2221 gaatttttaa ataaatagaa aatagtaaag cctcgttggg tggagtaatc cagctacgtc
     2281 ccaaacgccc tcgtccagca ctttgaaaat cagcccagac tatcgtccca gaaggatgcg
     2341 aatgccgatc caataggtaa gcattggtag agtctatctc tgcaaaatga accaccggaa
     2401 atctgagtat ttcagtagga atcggcaagt tgacgagccg gaaagaactc ataactttag
     2461 cgcaggtcaa gctcttcaat caactcatcg gcggaactat atttatccaa aataaaaaga
     2521 acatagagaa cgtcaacgct aatagcacgc gaaagagctg gattatattg ccagtcattg
     2581 gtcagcgctt cccagttgcc atcgaaggca caacccacca attctccccg cgcgtttaaa
     2641 actggactgc cagagtttcc gccggtgata tcgcaggagc tcagaaaagc caccggaaca
     2701 tcatttaatt cccgatcaat ccagcggcca taatcgcggc tattcgccag ctcgcttaat
     2761 ttttcaggaa catcaaaagg ttcagcgccg ctatgtttgg caattactcc ggctaaagtg
     2821 gtctggggtt ggtaaaagac ggcgtcgcgc ggctgatagc cttcgacgtt gccataagta
     2881 aaccgcaaac tgcggttggc gtcgggcaaa accgtcttac ccaaaacatt tttcaaggcc
     2941 gaaaggtact gcggtttgag aattaacatt ttgccgctta ttgttttctg gtctttctcg
     3001 atgcgctcca gttcgcagtt gactgcgacg gctaaagtta tcaatggatc ctgccgatcg
     3061 agtaaagact cggcgctcaa atcaaacatt gccagacatt cgtcggcatc ggtaagtcgg
     3121 gtatgagcaa aggcactatc aacataagcg gcgataacgg ctttagcaga atcggcaggc
     3181 tgatcctgaa tcaattcgcg caaaaaagca gggcgctcag ccgaagaaaa ttcggctaaa
     3241 tgatttagta ataatttcaa gatacgggca tcagccgggg catagtagcc gatgcgcctg
     3301 accttaaaat cacgcttggt acgagcaatt tggtaatcaa aaaaaccgta ttcacgttca
     3361 ctttccggtt tagccttttc gcgactccat ttttcgatag tcagcgcatc cgccagaacc
     3421 gtaacgtagc gtaaatattc aatgtacata tcgcgctcga atccggtata atattcatcg
     3481 taggctttct ggatttctgg cagaatattc ccatattgtt cttgccaggc aggcttgccg
     3541 gctatttttt gaactaattc cttctctact aacttttttt gtcccaaaat attttcactc
     3601 ttgaaaccag ctaataaacc ctgattattt ttcagcccat tgtaaaaacc ttttagtcgt
     3661 gaagatagga gtaagtcaac ctccggactt ttttggcttt catcttcaag ttcgttgatg
     3721 atttcttgaa agatccgaat gcgtagcgga tatactaaat tctgattata agccacggag
     3781 taactcgtga cattgcgata ggtgcgaccc ggatagccca aaatgaaaac aagctcatcg
     3841 ggattaagtc ctctggtaga aatcggtaga aaagctttag gctggtaggg tacatttttc
     3901 tcgctatatt cggccggctt gccgtcgggc gcgcagtaaa ttcgcataaa ggaaaagtca
     3961 ccagtgtggc gtggccacat ccagttatca acttcaccgc cgaaattgcc gatagattgc
     4021 ggtggagcgt aaaccaagcg aatgtcccga aatttccgat agtgataaag ataataactg
     4081 gcaccatcga gcatagcgac gacccttcct gtcagtccat nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn taaagataat aactggcacc atcgagcata gcgacgaccc
     4261 ttcctgtcag tccattggca gaatcttcgc tctcggcttc taattgggcg atagcgcgtt
     4321 ccaaagcgcg agtgcgttct tccggcgtca tttgcggggt aaaggattta agtatttttt
     4381 cagtgatatc ttcaaaacca atcattagcg acgcctcata gccgggcgcc ggcagttcat
     4441 cggctttagt gcgagcatag aagccttgtg ccaagtaatt ctgctcatcg gtcgaatttt
     4501 tctgaatggc accgaaagca acatgatgat tagtaagcac taaaccatct gccgacacaa
     4561 atgaaccggt tccgccgccg aataatagca ctgctccggc taaaccgcca ccgtccggtt
     4621 gatatatgtc ggcgggttta actttcaagc ctttgatttt ggtcgggatt tgatccggcg
     4681 gatacatacc ttccgcagct aatagtctga aattcaatgt caaaagacaa gtcgccagaa
     4741 aaaagagtcg ttttatgtta ctcatagttt ctttctctct ttttaattgt taaaagccac
     4801 gttctttgcg aatcgtttga taagcgtcgt tgatggattt gaatttttct tccgccagtt
     4861 tttgaaattc agggccaaga ttagctactt tatccgggtg atatttcacc gccaaatcgc
     4921 ggtaagcttt cttaattatc tcaacgtcgt cattcggttt aattcccaaa atctcataaa
     4981 atcggttagt tcccccgccg aaaatcgccc ggatagtttc gtagtcacgg tcagaaatcc
     5041 ccagagcatc cgcaatttct cgaatcactt gcaattctgt agtatggaga tcaccgtcca
     5101 cagcagcaat tccaaataag acatgaagca gttccatccg ggagtagtaa tccatattat
     5161 cgcgaacctg atgggcaatg tcatagatat tataatcttt accgagtatc tcccgataga
     5221 ggtagagtaa atcctgggca ttctgagttc cgaatttatc aaccaaaaat ttctttacgt
     5281 attgaacttc agaagatttg acattgcgat cggatttaga gacataggca aataaagcga
     5341 gcattgctac cgcgaaatct cccgcgcgag tatcgccgta agtcgcaaag gttgcacccg
     5401 gctcggcttt acctcgtttt ttctggactc cagattcgtt ggtaacaaca ttaccgatgt
     5461 aagccccgat caaagctccc agtggtccga aaagcgccca gccgattcca ccaccaatta
     5521 ttgttccagc taattttccc atattcttga tctcatttta aaatatttaa ctttagaaat
     5581 aataaatatt ccttgggtaa aatccaacat taaataaaaa tccctccacc tatgtaagag
     5641 tggagggatc ataactattt ggtataaata ctatcttaaa gcaaaaccga cacccagacc
     5701 ggcggcattg tattctccca tactatattc ggcattaata gatataaccg ctattttgat
     5761 attcaagcca gcggtaaaac gcgtatcatt cttacctttt aaatcgaatt tgatcttcgt
     5821 gccgtcttcc attttataag aggcggtgaa tttactggac tctttgccaa ccccaacata
     5881 cggcgttagc ataagaatag tcttactgac cattaaattg aaagccgtgc tgttggcgct
     5941 caaaaggtcg ccgatttcca ttttttgata ataaccctga acagcaatat ctaatgggaa
     6001 catcggtatc ggaatccagc gatcaacatc caattttaaa ccaccgccaa gagccgagat
     6061 ttcgccaata gcttcaccga tatcgccagg aattttaaat ttcgggatta ttcggataga
     6121 cggctcgatg cccatcggca atccaacgct ggcttgcaag atcgggaatg gaaatggtac
     6181 agtctttaaa tcttccagag ctttaattga cttcattcct gggatagatt ttacctgaat
     6241 tccgttaatc gcttcctgaa tttcgggagc catctcagta attgcagcat taatttgttc
     6301 tgctggcaag ccttgagcct gtaattgagt tctaagctgg gattcgattt cttcctggac
     6361 gtaaggttgg ttaaccgtga aaggcttcgc ttccccgcca aggatattgg gtgtggtgca
     6421 gtcaagaaaa atatcttcag gatccaaatt aattgaatag ctctgggaat tcttctcaat
     6481 attgaacgcg atatcgtcca atgccgaaag atcaaattga taggtagtct gactagcttt
     6541 gctggcgggt gctaaaccta ctccagtctt taattgaata tcaaatccca aagtgccgtg
     6601 cgtccccgca gtgttataca gaccggagtt catcgccgtc ccaaacccag tggctaacgg
     6661 ttgcagatat aacttcccgt tagtagcggc cagttcttta atctgatcca ttgagaattc
     6721 ctgtgccata atcgtggcaa ccataaaaag ggtcaataca acaatccggc taagtgttct
     6781 tctcatattt tcctccatga tttcattttt gctttaatgt tagggtcatt ttgagtaaaa
     6841 atcaagtaga attttagcga gccaatacta aagtcgtgat atactccaca gatttggcac
     6901 tggcaatatc attcgctaag caccgaacga tataaattcc aacgcgcgct tttgggctat
     6961 aatcactagc cccattccag taaattacgc cgctcgccgc tgtttcgagg ttcgtagcca
     7021 atcggtaaat caggcgtccc accaggtcat atatttctat agtaactttt gctgaaggaa
     7081 aaggtagtaa atattggatt ccgatttcat catcaatccc atcaccgtct ggcgaaaata
     7141 catctgccgt aggtttaatg ccaagtttat caagcttttc aggcagaatc acactgttag
     7201 gttgcccggg tgtaccgccg gcggcggcga cacttgcgcg ccaattcatc gcccgattag
     7261 aattatcaaa tgaaatacgc tccattgcta tcccttggat aattggccag gttgaattat
     7321 agaatagact gtcaattgcc cgaccggtcg gatcatatag gtaaacggca tcttcactat
     7381 tattcaaaga tggaaattta gcggttatcc aagtctgact tggtgctaaa tccggaaagt
     7441 tcagcaccga tgaatctcca gcaattacaa aatattcccc cggcgacaga aaataaccgg
     7501 acggaatgct ccggcgcacg gcggtattct ggtcggaaat catccaaccg gcaagatcaa
     7561 tggtattttc gctggaatta tagaattcaa gatattcggc aaccgaactc gaagctgtcc
     7621 gaatatccca aggatatggc ataaattcag atagaagtac gcttcctctt gaccaggaaa
     7681 tctgaatcga acgcaatagc atattattat cgttgatctg gtctgcaggc caatcaacgc
     7741 ggacagtgac agtgtgtttg ccggaggtta gcggaccaat ttcgaaattt ccactgtcag
     7801 cctgcccgag accaatacag tccagtggaa tctgagaaat gagatcattc tctaaagata
     7861 ttattaaatt tgcaccacag gcttggttct gccctttatt ggatatgaaa tagcacccgg
     7921 cgaaaacagt attcgtgtcg ccagtaatcg cggacagctg taatgaatcg agacttaaat
     7981 cattttccgg tcgggtaacg ctattgaaat atccggcggt ctgatgttcg ggactaaccg
     8041 aaagcgccca cgattgggag gtgtcactcg ccaggtgtgg caggattttt tcagccgaat
     8101 aaccggcttt tattggccag ccggctccat aatagagcga atcaataatt tttccactca
     8161 gatccttcaa ccaaatttga tcggcggaat tattcagacc cggccatttc cccgggataa
     8221 tgaccgcagc attgtccatc ttaaaataat tgaaacaggc gctgtcgccg ctcaagatta
     8281 catatccgcc gggtggaact tttccacctg aaatttcatt ttttaaataa cgattgccca
     8341 gcatccagtt ggatagatca attgtctcgt cgctgacatt aatacattca ataaactcaa
     8401 cctgattgct ggtaggataa gctaaaaatt cgttgagtgt taaacagcca gatggaaaag
     8461 ctaccactaa atcggtgcca gcataattat catcgagatt ctcatcaaag ctaaattcaa
     8521 gttctaccaa ataaagactt cgcccggctc tctcggcagt tatcttccca gtaacgatca
     8581 gggtcgagtc cgacttaagg ctctggttta tatcctgtcg ccaacaaagt tcatctgctt
     8641 gtctgcaact atcctgattt tgatccaaat agattttgat cgaaaaattt tcagctgaat
     8701 tgcgcccgac attcttgatc ttcaatgaaa aactcacctt ctgaccgacc gcatccagag
     8761 ttttagaaaa tttaaatgat gatggcacaa tggctaagtc attatccagt ttgataatgg
     8821 aattagtctg gccgggcgtg gctattatca gcgaaccgcc ccaattaccg gcattcaaag
     8881 ccggttgtgc tggatcaagt ctttccaggg agacgcccgt tccgccgccc cagctgtccc
     8941 gatagcgaac actatcaata atttgaccgg tagcatcata gaggaaaagt gaatcagttg
     9001 tgttattcag agtaggaaaa gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 naatagaatc tttggtgtta ttcagcgccg gcatagtctt aggacaatcc aggataaatg
     9181 gcacttgtcc ccaataactg atgaaagtgg ttgagtcatt aacaatcacg aggtattttt
     9241 gtggcggcag ataaaaatct tggtcggtta gcattatggt cgtactattg tcgctcagcg
     9301 tcaatctttt cagattgata gtatcggcgg aaatattcaa aagctccacc cactcgccgc
     9361 cgccgtcagt tgtcggataa tacataaact cactgattat cagacaattt tctgaaaaca
     9421 aaattttaat atccaatgac tcaagattat tgtcagaccg ctcatccgcc cgataaatag
     9481 cttccgccag aatagtgtaa attcccgatg gcaaacccgg tatcgtcgca ttttgcataa
     9541 cggaatcccc agctgtaagc gttattatct catcgtaaaa aatctcatct atttgtattt
     9601 gccggtcgga attttgatcc agtccacaga cgacttgtag agaatccgca gattttatgc
     9661 cgacattttt tacaatgaac tgcagatcaa tcggctgatg gggaaaaatt tgatcaactg
     9721 gagaaaccat tttcaatcct tcaatagcta catcataatc gcgaggcgta atgctgttcc
     9781 agaaacccgg cgtgccgcgc tggttgcgac tattgcccca gttagaaatt tgattagtgc
     9841 cctccggaat gattttttcc cagctgtagc cggatagggg cttcgcagtc gttagtacgg
     9901 ctgatattgt atctctctgg gcgttaaaaa ggtatatttg actggcaccc gaatttatca
     9961 ggccggtgtg tgccaaggaa ttatcctgga ttgtcaccag cagggtgttt tcaggaatca
    10021 ggtcttcgta agagctcgat ttataatcaa ctaaatagcc gcgatccata atgagagcga
    10081 aactatgcgg caaaagaaca tcaccataat cggcgacaaa acttaacgta tctacataac
    10141 cgtttatttc caagtagtaa ccgctaaggg taacagccag atcgccataa ttgtacaact
    10201 ccataaattc atcatagtag tctgacccga ctgggtcagc catcacttcg tttattgtaa
    10261 tggcttgacc atccaaaccg gtgataaaca cgtcgattat gaaaagcact gcggctgttg
    10321 cctttgttaa attcaaaaac tctccaactt cttgccgtta ttttatgtct taaatcacag
    10381 aaagtcaatg ataaattcat taagtatttc ctcgcttgta aaaaagttga ttttcagatt
    10441 tatttttagg aaaattagga gtattagatt tttaacaaat agaaaattta tgaggggaag
    10501 tgtgaaaaat ttaattttta gcaaattcgc atttttaaag aaaatggagc gtttcttatt
    10561 gatgcttggg ttgttcggga cattatggtc aaataatctc tatccccaaa gcgaacgcta
    10621 tactcgcaaa tcagtggcgt tcctggatgc attaatggtc actgattcca ggattcgcct
    10681 caatccggag gacgaaaaat acttattgac tgcgattcat aacgggatcc ggattgcccg
    10741 ctttgattat aaccctttac cggatatggt gcggtacact ttcaaagagc aactgcgagc
    10801 caaaggtgtt gtctccgaat cggaactcat cgccctaatc gaggctacca ttgttccaga
    10861 aatagtaaga atactcgata tcgaaaagga aattcgagcc caaaacttag ttgacgaaac
    10921 ccagcgtaat tcatttatct cgatcaaagc caaagaattg ggtattactg cggaccaaat
    10981 cgaacaagta atgaacgctt cgtacatcta ccttccgttt cttacgagct atacagtcag
    11041 caaacccaaa gatgacccaa acctgacggt aacaatcaaa ggtggtctat tctggtatca
    11101 tattgttccg ggcgatgatc cgtatctgga aagcatcgtg actctacaca ccgaagcctc
    11161 cggttccgcg gaaaaagcta aaagcgatgc tgaatccgaa gctttccggc tcgccgccag
    11221 tacttttgcc attaacttac aggttttgac ccgcgatatt gaaatgttta aactgagcgc
    11281 tccgatagcc gctatcgaaa agcgcaccgt gaaatttcca ctcggcacag cggaaggtat
    11341 caaactggat gaccccttct ttgttggtga atatttccag accagcggcg ataaaatcac
    11401 ttttaagaaa tcgggatttg tgcgcgtcag caaagttggc gataatcgtc aagatccgcg
    11461 acaactttct gcggcttatg ctattcaaaa gggagattgg gtaaaaggaa tgacaatggt
    11521 tgaacatccc cgggtaggtg ttgacgtggc tctaaagccg cgcgggtttt ttatgaatat
    11581 caacgaaggt gtttttgcaa gcgaggattt tttaatcttt tttgatgatt atgacggttt
    11641 tgctttcggc gccgacttag atttccagat caatattgct ccattaacta acaaacgtca
    11701 atcatttcta gttgcaggcg ccactgccgg agtagtgcca gttaaaagtg aattatatag
    11761 cactgatacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tcggcatttt
    11881 tgccggtttt caggcttttc cgtcatttga ctggtggacg attaaatacg gagacaaaga
    11941 aattgatgtc gctaactatg ctgatactga caagccttct atttccagct tgtcaccaac
    12001 tttcggcatt tacttccatt attcgccgcc gactttatca tttaacccgg catcagcgtt
    12061 ccaagccaat ggcaactaaa ctaaacgagg agtaaaatga aaaaattatc ggtaattata
    12121 ttaactattt tttgtattgc tagtttagtt ttcgccaaat ctgagaaatc aaagacgaaa
    12181 tcaaatcttc cgcgttcgca cgaggcggtc attttagaaa gcacttcacc ttctgaggta
    12241 atggtgcgtg ccaccggtta cagttccgag ggcggcaaag ccgccgaaat ggataaagaa
    12301 gccgaagccg atgctcgtaa agccgccgtc tggctgatct tattaggagg taccgatcca
    12361 cttctgcaga ccgatgccga aaaagctgaa ttcgctaaaa tccaggaaga atttttcgcg
    12421 ccctccaaca taaaacaatt catcgtttgg gaatccgatt attaccattc tcgcttgaaa
    12481 attgacggcg gtaaaacctt aaaaatcgaa aaaactttca aagtcaacaa aagcctaatc
    12541 cacgattatt tggtagaaag aggtattctt accaagactg ccgctattac tgaagcgctc
    12601 ggcttgccga cgataatggt tatcccggag acggacggta aacaagcacc aatcgaactt
    12661 ttgagaacca atccgaacct caaaaaaggt gcggaagtaa tcgaagccta cttgacggct
    12721 cgcaaattcg aggtaattgt acccgaacaa cagcaggcgc tccaggaatt aacttccgca
    12781 cattacaccc tccaaggagc cagcgaggat tacagttacc tgctggcact ctcaattggt
    12841 agtgatattt atattaccta taatgtcgcc gtcgaatctc gcctattagg ttcatcaaca
    12901 gtaaaaaagg gtgtggttgg ttgtcgtgcc tatgaaacta cgactggccg cttgctcggc
    12961 accgaaactg gctatagtcc ggaaagaacc gcgccggatg ccgttttaat cgaagaagct
    13021 atgaatgatg ccgtagataa agtcatcagc cggattatgg cttattggaa agaggatatt
    13081 cagcaaggag tgcaatataa agtgattttg aacatcagtg attccttcac caaatctgaa
    13141 gcagaagata tgatttacgc ttttggtgat ctgttgaaaa aggctagcaa aaggattaag
    13201 gaaaatgtaa tagctgatta ttcctatgac gttttgatat ggtgtgatcc tgctctttac
    13261 caatcctcat ccgatttgta tcgttttttc aaacaaaatt ataaaggcca gggtcgcctt
    13321 gagcgggtca gtttgaaccg caaactaatc ttgttaaaca tagtcgaaga ataagaggtc
    13381 atcgtgcaaa ggcaatcatt gactaaaata atattagttt tagcaggttt ggcgattaca
    13441 tcggcatcgg ctcaggttcc ggattggtac aaaaccaacc agcataaaaa atatcccgcc
    13501 agccgctatt tcctgggcgt tgccgtggca gatactaaag ccgaagccat cgaactagcg
    13561 cgggctgacg tcgctaagca gattcaggtg cgtatcgagt ctgaactcga aaccatcgag
    13621 agcgaattca gagaaggcga caaaaattac ttaaaagccg aaactaccaa tcgcactaaa
    13681 tccattgtcg cggaaaccgt ctcgggaatc gaaatcgccg aggtcaaaga ggtcaagggc
    13741 aaaacctacg tcctcgccgt gctcaataag gaaaagtatc tctccacttt agaaatccag
    13801 atggatgaga tcctgatgaa aagcgaacaa cttatcgcga gcgcacgcca gatggtcgcc
    13861 gacggtcgaa ttctaaacgc gctgaataat ttcattgatg cccagaatac aatcccggat
    13921 ttctatacaa aaagttcatt gtacaccgcc ctttccggtc gcaagtatcc caatttcaac
    13981 cagttcaccg gaccaggaat agttgcggaa gtgcgtgaaa ttttagcgca tatcgaatta
    14041 aaaattctgt cgggaaataa tcaaattgcc gtcgccggtc agaatttgcc cacacccatt
    14101 aaagtgcagg ttgtctatgt tcgcgacggc gaaatcatcg gagtcgccgg ttttcccgtt
    14161 atagctagat atgatggcgg tgaaattatt actaaaatcg aaactgacgc gaacggaatt
    14221 gctgaattta attgcgtagc tactccaacc gacaatgtcg gccaaagcgg ggcggttaaa
    14281 atcgccctgg atttatcgaa aatcccagaa cagtatcgcg atgcgatgac tcgctccgca
    14341 gttcagatca gctacatcct gactgctaat gatctaaaat ttacggtcga ggttattcag
    14401 aaaaacagcc aacacctgac attcatcgaa gacgagctag tgcgattgat cgccgctaat
    14461 ggctatactt gttcagcgga tgcgccctgg ctagttcaag caactttggt tattatctct
    14521 cagaaggagg ttccctcgcc ggctggcaat cagcacttgg tagaagctca gttccaatta
    14581 cgcttgatag aaaagcaaac tggcaacgtt tttgccgcgc tggatattaa tactaaagga
    14641 ctcgattcac agtcgcccga taatgcgctc tccaaagccg ctcaaaacct aaaaattcca
    14701 agtaataaat ttgccgcttt tttacaaagc gccaatcagc ccaaaaatga ttaattccaa
    14761 aaagtaacaa aaggagaaag tatgaagaag ttactcgtat ttgttttgtt aatttccgta
    14821 gccttgttca cggtcaattg tggcggtaaa cagcctaaag ccaaaatgga tgatatccct
    14881 gaatggtatc taaatccgcc tcaagctgaa gatgccatct atcaagccgg tgatgcggtt
    14941 aagtccagca tggcccttgc caaagacgcg gctgatgctc gcgctcgcga cgctattgcc
    15001 cggacagtgg agctcagagt caccaacatg atgaaagact ttatgcaaca aagtggtatg
    15061 ggagaggatg ccgaagccct cgaattcact tcttccgtta gcaaacaggt cgccaacacc
    15121 gttctgaaag gttgccggat tgtcaagcgg gaaatgaagg tcgaaggcaa taactaccac
    15181 gcttattctc tcgccgagta taacctgaac tcattgattg aggaaactct taacgaagcc
    15241 cggcggcaaa aagctcttta taatgaagcc aaagccaata tgagcttcga agaactggaa
    15301 agagaaatcg aaaaacttaa aaacgtcggt caatgattga accgttaaag cccgtttggc
    15361 tctaaccaga cgggcttttt tttctcaatg aaattttgcc tttttactat tgccctggtc
    15421 cttgggctaa aaaatctggc gtttgctcaa gaatgtgtcc cttcttggtt tgagaaattg
    15481 cccgaagtag aaggaatccg tctggcagtc ggatattcgg gtaaattcac caacgaagcc
    15541 ggcgctaaat ccgccgccgt ccaattggct cacaaaaata tgactaagca aatttccatt
    15601 cgactgcaat ttgacatcga ggaactggct gatggacgaa tacgcctgtt gaatccatct
    15661 tttaaagaat tctatgaagg ttcaatttta aatgcagttg aaagtaactc ttccgttatt
    15721 gactcaacaa taaccgtgga gggttatttc gttttattgg catttccagc aatcgaatac
    15781 ctgcccattc ccaatgcttg tgacaagcca tggaacatac gaccggactg gattgaaaat
    15841 cttccccaag aagatggcta tgtctatgga attggtattg tcgcccgata ccgcaattgg
    15901 gtgcgcgcct ggcaagacgc cgatgaatac tcccgcttcg atctcggcaa gaatttgcag
    15961 gttaatactg agtcaatcca cgccgtccaa cgtaacaatc gcaccacgat tgaatcggtg
    16021 attttgaaac agacctatga tctgacaatc aatgatgcta cgatcgtcgc tcgctggtta
    16081 gatactgcta acggtgtcta ttattcgctc tgccgagcac ccagaccttg aggggcattg
    16141 ggcactgggc attagtcatt ggtcattagt cattggtcat tagtaaggag actgagtaaa
    16201 gaggttgaga aaagagaatg gtcattagtc actggtcatt ggtcattggt cactggtcat
    16261 tagtaaagag actgagtaaa gaggagcacc caaaatctga ccattcaatt tacttacggc
    16321 attaagaaca tctcgggctg ggaagcgaag tcccagcggg aaaaatggaa tttttagcgc
    16381 tgtaatagca ctttcgtaat tatccattac cctttccttt atcactaagg ttttatgaac
    16441 ttgatgactt ttgcgcctta aattccattc gctgggactg aacgaacccc cgtgagatgt
    16501 tctttagccg ttgccttttt tggttctttt ttcggcaaag gaaaaaaaga acaggaaggt
    16561 tagtcattgg ccattggtca ttggtcatta gtcattagta aagaggctga ggttgagaaa
    16621 agagactgag gctgagatta gtcattggtc attggtcatt agtcattagt aaaaaggcta
    16681 agactgaaga agaacttaaa tcttagcctt ttttctcagc cttaaccttt cttaagctga
    16741 ggctgagtaa agagattgag taaatagatt gaggttgaga aagaattcaa atcttagcct
    16801 tctttctcag tcttaacctt tctttaacga gtttacaact cgattggtgg gattaagctg
    16861 gctggtttct cgcatggctt tcattttgcc aataaaattg cgaaattagc
//
LOCUS       DAOSMP010000042        16113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_3653_length_16113_cov_10.589550, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000042 DAOSMP010000000
VERSION     DAOSMP010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 16113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16113
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3653_length_16113_cov_10.589550"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PKX09_07635"
     CDS             <1..662
                     /locus_tag="PKX09_07635"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOU17729.1"
                     /translation="RVALGRAIVRKPKVFLFDEPLSNLDAKLRVQMRTEIKKLHHHLK
                     TTMVYVTHDQVEALTMGDRIVVMKDGVIHQIGSPSEIYQNPQDLFVAGFIGSPAMNFI
                     PGVLHQGVFQSNTNNIKLILEKSPYKEMDNVAVVLGIRPEDVHLNKQDAQYQEVRLIL
                     EVIEPLGNESIMYFSDEGHPINLRTQDTNISHTIGEAIPVFFNVDKVHIFDQKTEVAI
                     R"
     gene            2039..3001
                     /gene="cas6"
                     /locus_tag="PKX09_07640"
     CDS             2039..3001
                     /gene="cas6"
                     /locus_tag="PKX09_07640"
                     /inference="COORDINATES: protein motif:HMM:NF021551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HOU17730.1"
                     /translation="MCASHISGFRLGEFTLYLKGIEEIKFNRFPSATLRGAFGTSLKS
                     TLCIHSAINANCEKCLIKKTCAYYYLFETGTDLHGENNPHPFVILLPLELANRTILPG
                     EIFSFHLVLFGYGLDYLPHIIYAFENLGKKGLGSRKGKFDLVEVKFVNQEAEKIIYFE
                     SDKILNNEDQNYLFEFHNDNGVVNQIKINLLTPLRMKNEGRLVTEVNFKIFIINLLRR
                     ISKIAEIHCGEEIAIDWPELLKIGEETKVISDNTYWKEYKRFSIRQKQEMLLGGLLGN
                     FEVQGKFRELLPYLYLGEIIHVGKSTSFGFGKYQMQIIKKEESL"
     gene            2998..5715
                     /gene="cas10"
                     /locus_tag="PKX09_07645"
     CDS             2998..5715
                     /gene="cas10"
                     /locus_tag="PKX09_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Cas10/Csm1"
                     /protein_id="HOU17731.1"
                     /translation="MMNDKIRESIILGALLHDIGKFWQRCEEKGGIDNASFLDSNVRN
                     TISNDICPGRKYGDVTILTHQHVAWTYQFLTKFESKIYPTGTSDEESILQFASGHHRS
                     IPSTSLQTLIQVADWISAAQDRNGDTGDIKDELSRSYNYRKQRQRPVFENLFRDDYDS
                     RGSTYRYNIGELKLDEILMPQDFKNEDRKLSSEYQKLWENFVAEFINIPNKDFNAYLE
                     TLLCLLQKYTWCIPSSTNDSPDISLYDHSRSTASIATCLYDYLQETNPTLIQPGEKTQ
                     QQKTKAFFTNNEKFALLITADISGIQKFIYQISSVKAATALKGRSFYLQLISDAAARY
                     FLTQLNLPYTNLIYSSGGNFFLLAPNTKKVQLEIARTTEQINRSLLAKYDSNLFLGVG
                     YEELSGSDFIHNLSEKWEKAIRKANSQKRRKFRDSVESDWYFFQPRPKNNAKPCDNCG
                     ADCNEAELSKIKEEEEEKYCPLCREFIQAGTRLKSAQILLEFVTSEANELDYNEIIYP
                     ITGVPVGYILTGSTQLDQNAGIISQAIFKKIYYLNQADFIGKLSGERFTTFAWGFKFY
                     GGNHLPLNDQGEPSTFDYLAGVRENKAKETISFKRLGILRMDVDNLGKIFSKGFLLNS
                     LHNPKISNESVKAPYSISRISTLSSMLDIFFCGYLNTLHQQNEDFKNNLYLLYSGGDD
                     LFIVGRWDKVIDFAALIREEFKKFTCHHPDISISGGIVLTPPKYPIHRSADLAGDAES
                     ASKSKRLINGNVKEKDAISFFGKTLSWNDFEVAKALKDDLVQWIENPSEGKTKMNRGL
                     INRLNQIYSTYQKEADLVKKQEKEGKIKIEEVADRIQYNKWRWRLVYSLKRFLNQNEN
                     YNNEIENIQKALIDSNIYRGQLSEQPIIEYLDLSVKWAEYQLRTNKKED"
     gene            5717..6196
                     /gene="csm2"
                     /locus_tag="PKX09_07650"
     CDS             5717..6196
                     /gene="csm2"
                     /locus_tag="PKX09_07650"
                     /inference="COORDINATES: protein motif:HMM:NF015695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Csm2"
                     /protein_id="HOU17732.1"
                     /translation="MEIENPKIDPNLVTIKKLTEFGKSNVKEWLRNGINKDSLKFCEE
                     FGFCLSRYYENEKPKPLSTNQIRNVYGEVKRIEMLLGSDPKEKEWKTVESKVLLLKPK
                     MAYSAKRDKSIPAKCLYEIVKASIEVVENSNLEEKITSFQNFAKLFEAIIAYHRAYD"
     gene            6233..6946
                     /gene="csm3"
                     /locus_tag="PKX09_07655"
     CDS             6233..6946
                     /gene="csm3"
                     /locus_tag="PKX09_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm3"
                     /protein_id="HOU17733.1"
                     /translation="MKLIKKIFIEGKITAQTGLHIGGSDVGLSIGGADSPVIRDPITN
                     VPYIPGSSIKGKIRSLLEKVDPTIQYNKLVGSARIHQCQVEEEKDVCLICRLFGLPAE
                     EEKANTPQRLIVRDAMMQPEWVDKLFKSKNTDMPYTEVKTEVVIDRVTSAANLRQIER
                     VPAGAVFELEMILNIYDSDIQDDLMKLLEKGRRLLNDDYLGGSGTRGYGKVNMTFAEI
                     KYKDEKIYQGDNQPLPLEG"
     gene            6947..7966
                     /gene="csm4"
                     /locus_tag="PKX09_07660"
     CDS             6947..7966
                     /gene="csm4"
                     /locus_tag="PKX09_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm4"
                     /protein_id="HOU17734.1"
                     /translation="MQFTIFKLKFRTPLHIGDNRPNYESCERFVHSDTLFSALINTWK
                     LLYPDDLDVFFPESAPASISFPYFRISSAFPFAGEELFFPKPFYSPPYPQDGDSGDAK
                     TFKKLIWIGKNHLEQWLKGESLKIEDACLSPDKHFLFSDKIPKYQPNPVIVQDSPRIR
                     KDRETEQTEIFYYSRLYFAENAGLYFLATFQNEEWERKFNAVLNLLGDEGLGGDRSVG
                     NGLFEVIERKEGFSLILPEKSNHFLTLSLYHPKSGEMSKNLDNFYYELINRSGWVYSG
                     RGHSLRRNNVRMFSEGSVFLGSGNERGDVVKVLDPLPERNLPHAVYRYGQVLSLPISV
                     AEEAL"
     gene            7963..9096
                     /gene="csm5"
                     /locus_tag="PKX09_07665"
     CDS             7963..9096
                     /gene="csm5"
                     /locus_tag="PKX09_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm5"
                     /protein_id="HOU17735.1"
                     /translation="MNNKNITKKFRITVLTPVHIGTGFRKMKGIDFYFDERTSQVILV
                     DLDEIGTLLVDNSQALNELANLDEQKDIGDLTRRYHLNLKNNKNYPINSVGREFLEFV
                     RTGLGQPLIPGSSLKGSLRTAILRNIIDQKSDVERRELFKEIPNGGNPKSASDKLLKK
                     IFGSNPNYDLMRVLIISDSHFSEADLMLIESRVLSQNRNGWHWKPNPKNPNQPMQVWC
                     EALRPNSKAEISLSLDNFLLTDSRAVWELKFPNKLAADLHTLIDQINQYSAQMIDAEI
                     TYFDERDSEKPELNEIIRNLNLVKKHIPANDSAMVIRLGWGSGWKHMTGDYLERDKDI
                     LEQIRMNFRLGRPGMPFPKSRKIAFQNGKPIFPFGWIKLEVLN"
     gene            9093..10556
                     /locus_tag="PKX09_07670"
     CDS             9093..10556
                     /locus_tag="PKX09_07670"
                     /inference="COORDINATES: protein motif:HMM:NF021207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17736.1"
                     /translation="MKAIYLANVGNRDVKFDNQNIKVPRLSGKDYLDRFEKVAEHLTF
                     PIITKGLECVFQDLQDIPLEQLILFYTDQDQNEVSEQHYQSDTLYFADLIEKLVNLRI
                     PEKVKNVVKIKICHSPNDYDFTYEFFSSRLTEIENEVKPDRIYLSPAGGIPACNMNLL
                     LQGSRIFTRRAVTLLIPENPDKPAKLQKLGDLINRENNRLIIQRLLSEYDFGGAAEVL
                     ENESDDKSKSLLALARSVQYRLYFDFQTALNYLSNVNPEVLQIAETNIANFGTVENLI
                     NTIQENFTALIKPIEPLHGNQTDFDQKNKWLELQKRLNAEVLLNVRIKWLSKQYIDFL
                     GRIFRLQEGVLRWIFEKETGYSADSEQFPNDYPDFLKQNPEFKDYLKNESINGKNPVA
                     NRLNRGTLLLYLRFLSKIRNEYKPVLKQCDKLDHLSALRNKSALAHGNEPVNDCIISQ
                     IYGKNVLGNLSKLASTFGLEDHLPQIDCIRNIIIENF"
     repeat_region   11097..12814
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=11097..11133
                     /rpt_unit_seq="gtgataatcattgacccgatgataaggggattaagac"
     gene            13751..14917
                     /locus_tag="PKX09_07675"
     CDS             13751..14917
                     /locus_tag="PKX09_07675"
                     /inference="COORDINATES: protein motif:HMM:NF040341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6174 domain-containing protein"
                     /protein_id="HOU17737.1"
                     /translation="MRKSVIVLILLFLINCEKDINNPIIEPEPGIGKTTILIERGLVD
                     YNSYNFPIVSLTYAKISISDIDTSLYFAMSESSPNDTIVILSHGVFNGELNTTGIPIA
                     DECLFIKAYFRNEDTSGTVLLYENLWNYSFIDSVIFEPYKLIGLSDTIRIGKQRYLSW
                     KENYRIDTLKFNVYYKPNINIVYDRFSLSTMDFLYKNSAYDSVKYIAKFIVGIPSMTS
                     EPNIVYYPYSTIELVYESSILKVISRLNYQVEYKLPTWKLNHVIDSLEICRNNWKSLS
                     IHDYSIYQTIKSWYPWYGDTLKIAVYSDTINDIQKVPDLSTIDRSVWERYKTIDELFE
                     LAGLDSTRYELRVRYNSDYYYPEFLYYELKPPIYTEGGFEYYTWNLEIRRLLVS"
     gene            14996..15850
                     /locus_tag="PKX09_07680"
     CDS             14996..15850
                     /locus_tag="PKX09_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17738.1"
                     /translation="MRKNWIHESHKSGATHSYLNVKWMRYRKFALIIYSFSLINCSPF
                     ENNLFPLEGRIFFKISEDYKQYNRENNPEIFLFLETEKIYGCFNYGISTDCDIDGRKI
                     EVDILGINQPGVCLTAIGPAIARIHLPLSNKLYSLKISGDDFTNNYAVIVTDSSISVT
                     GDSTVNTKPEIELFWRYPPNSFVYLYGSTFEDSSLANVFLDTLMQVIDIAEFYFPQYG
                     EIPYPKSSQGHYWDMPARYFYYNSEDDFNKIEDILRDFKSIYIQDKQGISVTIRNWRN
                     RKFQSWLL"
     gene            15962..>16113
                     /locus_tag="PKX09_07685"
     CDS             15962..>16113
                     /locus_tag="PKX09_07685"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOU17739.1"
                     /translation="MNQNRIQHAASKKVEILREHLENQVPISEIARCYGINPNLIHKW
                     KKQLFEG"
BASE COUNT         5324 a         2814 c         3147 g         4828 t
ORIGIN      
        1 agcgagtagc tctcggtcgg gcaattgtcc ggaaaccaaa agtcttttta ttcgatgagc
       61 cactctcgaa tctggatgca aaattgcgag ttcaaatgcg caccgagata aaaaaattgc
      121 atcatcattt aaaaacgact atggtctatg taacccatga tcaggtggaa gctctgacta
      181 tgggcgatcg aattgtcgtg atgaaggatg gagtaattca tcagatcggt tcaccttcgg
      241 aaatttacca gaatccgcaa gatttattcg ttgccggctt catcggtagt cccgcaatga
      301 atttcattcc aggtgtttta catcagggag ttttccagag taacaccaat aatatcaaac
      361 ttatcttgga gaaatctcct tataaggaaa tggataatgt agcggtagta ttgggcatta
      421 gaccggagga tgtccacttg aataagcaag atgctcagta tcaggaagta agattaattc
      481 ttgaagttat cgaaccgttg ggcaatgaat caattatgta tttttcagac gaaggacatc
      541 caattaatct gcgtactcag gacacaaata tttctcatac catcggagaa gccatccctg
      601 tattctttaa tgtagataaa gttcatatat tcgatcaaaa aacagaagta gcgatccgat
      661 aaactaaaaa tcgtgtctat caataatcta aagtcacgtc cagaaaattt ataaataagg
      721 tattataagg ctctcgtaag actatcatct atagctaatc ttgaacgagt tgttgtaaga
      781 tttagcccac accgcggagc ggttgaagta ttgtagtaag acgaaaccgc ataaaagtta
      841 ttcctcggag aggatcgaga agaaaatatt tgccgaacgc aatctttatt tgaccaagaa
      901 taactattgc gttatgcgct tggctaatat ttcaacaaga ttttatctgc cagattatca
      961 gaacttttcc ctaattatat agaaagttaa acctctccga ggtagcaatt tctggcgatt
     1021 atgtttttct acaaaactta aagccctacg ggctagttgg gaatctatgg ttttcagata
     1081 aacaattacc gcggagcggt cgaagtattg tagtaagacg aaaccgcata aaaattattc
     1141 ctcgtggaaa atagaggaga aactatcatt tccgaatgat tttcatagat tatcaaatga
     1201 caaaacaatt ctatttaatg acttgatcaa acatataaat gatttaacaa tttgatttat
     1261 tggtacaatt aaatggccaa acgatttggc caaacatttt gaccgtctga taaaaggtcc
     1321 aaacgatttg gccactttgt ttaccggtaa aaataaatgg ccaaatgatt tggccactct
     1381 attttaccat ccaactaaag ttccaaatga cttaacaatt taatttaatg gtctgatcaa
     1441 atggccaaat gactaaaatg ttttaaaaag tgactttttt aaatggatca accgtttgaa
     1501 aactatctca agccgtcctg gctagtcatc aaataattgg gaataactaa agaaatgatt
     1561 ttgatatttg ccacaggaga aattttaatc cctgcagagt aaaccgactc gccgcaggcg
     1621 gagctaaatt ggtttcggtg catccttcgt tttactaaaa attggttttt ccagaacgac
     1681 actttaagac ctttgatcta ttttggattc tatagttgtt agattgacac ttaccgcata
     1741 gcagtcaaag tattttagaa aacatagaaa ccaaaaaagt tattctctaa gagacagata
     1801 gcaaaccacc tcccatcctg atcagtcgag atgaactttg ttatttgttt gaaactttat
     1861 caatcagatt atcaataagt tagcaaatta ctatataaaa tatcagggga agttctgaaa
     1921 ataaatgatt gactttcgtt caataatttt ataaaatcca tagaacggaa atatttaaac
     1981 cgacgtgttt ttttatttga gttaatgaat gtttttcttt aacgggagga attcataaat
     2041 gtgtgcttcg catatttcag gatttcgatt aggggagttt accctttatc ttaaaggtat
     2101 tgaagaaatt aaatttaata gatttccgtc ggcgacctta cgaggagcct ttggaaccag
     2161 tttaaaatct accttatgta tccattcagc gattaatgcc aattgtgaga aatgtcttat
     2221 caagaaaacc tgtgcctatt actacctttt tgaaaccggt acggatttac atggagaaaa
     2281 taatcctcac ccttttgtaa ttttactacc gctagagcta gccaatagaa ccatattacc
     2341 tggagaaata ttttcatttc atttagtttt atttggatac ggtctggatt atcttcctca
     2401 tataatctat gcttttgaaa atctgggaaa aaagggactg ggaagtagaa agggcaaatt
     2461 tgatttagta gaggtaaaat ttgttaatca ggaagccgaa aaaattatct atttcgagtc
     2521 agataaaatt ctaaacaatg aagaccaaaa ctatctattc gaatttcata atgataatgg
     2581 agtagttaat caaataaaaa taaatttgct aactccgcta cgaatgaaaa atgaagggag
     2641 actagtaact gaagtcaatt tcaaaatatt cataatcaat ctactgcgtc gaatctcaaa
     2701 aatagccgag attcattgtg gtgaggaaat agcaattgat tggcccgagt tgcttaaaat
     2761 tggagaagaa actaaagtaa tctcagataa cacctactgg aaagaataca aacgcttctc
     2821 tattcgtcaa aaacaggaga tgttattagg cggcttactc ggaaattttg aagttcaggg
     2881 caaatttaga gagcttttgc cgtatttata tcttggagaa ataatccacg tcggtaaaag
     2941 cactagtttt ggctttggaa aatatcaaat gcaaataata aaaaaggaag aatctttatg
     3001 atgaatgaca aaattcgcga aagtatcatt ctcggagcat tgcttcacga tattgggaaa
     3061 ttctggcaaa gatgtgaaga aaaaggtgga atagataatg ctagttttct tgactcgaat
     3121 gtaagaaata cgattagtaa tgatatttgc cccggtcgga aatatggaga tgttacaata
     3181 ttgactcacc agcatgtggc ctggacatat caattcctta caaaatttga atcaaaaata
     3241 tatcccactg ggacttctga tgaagaatct attttgcaat ttgcttccgg tcatcataga
     3301 tcaatacctt ctacttccct tcaaactcta attcaagttg ccgattggat ttcagccgct
     3361 caagatcgta atggagatac tggagatatc aaagatgaac taagccgtag ttacaactat
     3421 cggaaacagc ggcaacgtcc agttttcgag aatcttttta gagatgacta tgatagcaga
     3481 ggaagtactt atcgctataa tatcggagag cttaaactcg atgaaatcct tatgccacaa
     3541 gattttaaaa atgaagatcg aaaattatct tccgagtacc aaaaactatg ggaaaatttc
     3601 gtagctgaat ttataaatat tcctaataag gattttaacg cttacctaga aactttgctc
     3661 tgtcttttgc agaaatatac ctggtgtatc ccaagttcta ccaacgatag tcccgacatt
     3721 tcactttatg atcattctcg ttcgacagca tctattgcta cctgtctata tgattatcta
     3781 caagagacta atccaacttt aatccagccg ggagagaaaa ctcaacagca aaaaaccaaa
     3841 gccttcttta ctaataatga gaaatttgct ttattaatta cagcagatat tagcggtatt
     3901 caaaaattta tttaccagat ttcatcggta aaagccgcta ctgccttaaa agggcgatcc
     3961 ttttatttac agctgataag cgatgccgcc gcacgctatt ttcttaccca attgaatctt
     4021 ccttatacaa acttaattta ttctagtgga ggtaatttct ttttattggc tcctaataca
     4081 aaaaaggttc aattggaaat cgccagaact accgagcaaa taaaccgtag tctcttagct
     4141 aaatacgata gcaatttgtt tctcggcgtg ggttatgaag aattatcagg ttcagatttt
     4201 attcataatc tatcggaaaa atgggaaaag gcaatccgaa aagctaactc tcaaaaacgc
     4261 cgtaaatttc gcgattcagt tgaaagcgat tggtattttt ttcaaccgag accaaagaat
     4321 aatgccaaac catgcgataa ctgcggcgct gattgcaatg aagccgaact aagtaaaatt
     4381 aaagaagagg aagaagaaaa gtactgcccg ctttgtcgag aattcatcca agccggaacc
     4441 cgactgaaat cagcccaaat tttactcgag tttgtcactt ctgaggcaaa cgagctagat
     4501 tataacgaga ttatttatcc tataaccgga gtgccggttg gttatatttt aaccggtagc
     4561 acgcaactcg atcaaaatgc cggaatcatt agtcaggcta tttttaagaa gatatattat
     4621 ttaaatcaag ccgattttat aggaaaatta tcgggagaac gatttactac ctttgcctgg
     4681 ggattcaaat tctatggtgg caatcatttg ccgctcaatg atcagggaga accttctact
     4741 tttgattatt tagccggcgt acgagaaaat aaggcaaaag aaactatctc tttcaagcgg
     4801 ctcgggattt tacgtatgga tgtagataat ctgggaaaaa tctttagtaa gggctttcta
     4861 ttaaattcgc tccataatcc taaaatttca aatgaatcag ttaaagcgcc ttattcgatt
     4921 tctcgaattt ctaccctcag ttctatgctc gatattttct tttgcggcta tctgaatact
     4981 ttgcatcagc aaaatgaaga ttttaaaaac aatctgtatt tactctattc cggcggtgat
     5041 gaccttttta ttgtgggacg ctgggacaaa gtaatagact ttgccgcttt gattcgagaa
     5101 gaattcaaaa agtttacctg tcatcatcct gatatatcaa tctccggagg tatcgtactg
     5161 acgccgccaa aatatcccat tcatcgttcc gctgatttgg ctggtgatgc ggagtcagcc
     5221 tccaaatcca aacgtcttat caacggtaac gtaaaggaaa aagacgcaat aagtttcttc
     5281 ggcaagactc ttagctggaa tgatttcgag gtagccaagg ctcttaaaga tgatttggtt
     5341 cagtggatcg aaaatcccag cgagggtaaa actaaaatga atcgaggtct tattaaccgc
     5401 ctcaatcaaa tatactcaac ctatcaaaaa gaagcggatc tggtaaaaaa acaagaaaaa
     5461 gaagggaaaa taaaaatcga agaagtcgct gatcgaattc aatataataa atggcgctgg
     5521 cgtctggtct attctctcaa acgcttccta aaccaaaatg agaattacaa taacgagatt
     5581 gaaaatatcc aaaaggcact gattgatagt aatatatatc gcggccaact aagcgaacaa
     5641 cccataatcg aatatctcga tttgtccgta aaatgggcag aatatcaact aagaaccaat
     5701 aaaaaggagg attaaaatgg aaatcgaaaa tccaaaaatt gatccgaatt tagtaactat
     5761 taaaaagtta acagaattcg ggaaaagcaa tgtcaaggaa tggctaagaa atggcattaa
     5821 taaggattca ttaaaatttt gtgaagaatt tggattctgc ctatctcgat attatgaaaa
     5881 tgaaaaacca aagccactat caacaaatca aatcagaaat gtgtacggtg aggttaaaag
     5941 aattgaaatg ttattaggta gtgatcctaa agaaaaagaa tggaaaacag tagaatctaa
     6001 agtgctatta ctcaaaccaa aaatggccta ttcagcaaaa agagacaaat caattccagc
     6061 taaatgcctt tatgaaattg ttaaggcaag tatcgaagtt gttgaaaatt caaatttgga
     6121 agagaaaata acttcctttc agaattttgc aaaattattt gaggcaataa ttgcctatca
     6181 tcgtgcttat gattaaagta tgtagaaata ataattaaag gagaaaaata aaatgaaatt
     6241 aattaaaaaa attttcattg aaggtaaaat tactgctcag actggactgc atattggcgg
     6301 ctcggacgtc ggattatcca tcggtggtgc cgattccccg gtaattcgcg atcctattac
     6361 taatgtcccc tatatcccgg gctcatcaat caaaggtaaa attagaagcc ttcttgaaaa
     6421 agttgaccct actattcagt acaacaaact ggtaggaagt gccagaattc atcaatgtca
     6481 agttgaagaa gaaaaagacg tatgcctgat ttgtagattg tttggtttac cggcagaaga
     6541 agagaaggca aacactccgc aacgtctcat cgttcgagat gctatgatgc aacccgagtg
     6601 ggtggataaa ctctttaaga gtaagaatac cgatatgcct tatactgaag taaaaactga
     6661 agttgtcatt gatcgggtca cttctgccgc taatctacga caaattgaac gtgttccggc
     6721 tggcgcagtc ttcgaattag agatgattct taatatctac gatagtgata tccaagatga
     6781 tcttatgaag ttacttgaaa aaggccggcg cctattgaat gatgattatc tcggtggtag
     6841 tggaacgcgt ggttatggta aagtcaatat gacctttgcc gaaatcaaat ataaagatga
     6901 aaaaatctat caaggagaca atcagccact cccactggag ggataaatgc agtttacaat
     6961 ttttaagctc aaatttcgga cgccactgca tatcggcgac aaccgaccga attacgaaag
     7021 ttgcgaaaga tttgttcata gcgatacgct attcagtgcc ctgataaata cctggaaact
     7081 gctttatccg gatgatcttg atgtcttttt ccccgaatcc gctccggcat ccatttcttt
     7141 tccctatttt cggatttcat cggcttttcc atttgccggg gaagagcttt tctttccgaa
     7201 gcctttttac agtccaccgt atccgcaaga tggcgattct ggcgatgcta agacttttaa
     7261 gaaactgatc tggattggaa aaaatcatct ggaacaatgg ctcaaaggcg aatcgttaaa
     7321 aatcgaggat gcttgtcttt cgcctgataa acatttcctc ttttctgaca aaataccaaa
     7381 ataccagcct aatccagtaa tcgtgcagga ttcgccgcgg attagaaaag atcgggaaac
     7441 cgagcagacc gaaatcttct attattctcg cttgtatttt gccgaaaatg ccggcctcta
     7501 cttcctggca accttccaaa atgaagaatg ggaaagaaaa ttcaatgccg ttctgaattt
     7561 actgggtgat gaaggactcg ggggcgaccg ctcggttgga aacggacttt ttgaagttat
     7621 cgaaaggaaa gaaggattct cactcattct accggaaaaa tcaaatcatt ttcttacact
     7681 ttcactttat catccaaaat caggagaaat gtctaagaat ttggataatt tttactacga
     7741 acttatcaat cgtagtggct gggtttactc tggtcgtggt cactcgctaa gacgtaataa
     7801 tgtccggatg ttttcggaag gctctgtatt tttaggttcg ggaaatgaac gaggcgatgt
     7861 cgtcaaggtt ctcgatcctc tgccggagcg gaatctaccg catgccgttt atcgctatgg
     7921 ccaggtcttg agtctgccga ttagcgtcgc ggaggaagca ctatgaataa caaaaacata
     7981 actaaaaagt ttagaattac cgtcctgacg ccggttcata tcggtactgg tttccgcaaa
     8041 atgaaaggca ttgatttcta cttcgatgaa agaactagcc aggttattct ggtagattta
     8101 gacgaaatcg ggacacttct ggtagataat tctcaggctt tgaatgaatt agccaatctc
     8161 gatgaacaaa aagatattgg tgacctgact cgacggtacc acctgaactt gaaaaataat
     8221 aagaattatc caattaatag tgtcggaaga gaatttctgg agtttgtccg caccggcctc
     8281 ggtcagccgc tgatccccgg cagctccctg aaaggttctt tacgtacggc tatcttgaga
     8341 aatattattg atcaaaaaag tgatgtggaa cgtagggaac tatttaagga aataccaaac
     8401 ggagggaatc caaaatcggc gtctgataaa ttacttaaaa agatttttgg tagtaatcca
     8461 aactacgatc taatgcgcgt tctgataata agcgactccc atttttcgga agccgacctg
     8521 atgctaattg aatcacgcgt cctatctcag aatcgtaatg gctggcattg gaaacctaac
     8581 cccaaaaatc ctaatcaacc gatgcaggtc tggtgtgaag ctctgcgacc taatagcaaa
     8641 gcggaaatca gcctatcatt ggataatttt ctgctgaccg attccagagc agtctgggaa
     8701 ttaaaatttc ctaataaatt agcggctgac ctgcatacac ttattgatca aattaatcag
     8761 tattcagccc aaatgattga tgctgagatc acttattttg atgagagaga tagcgaaaaa
     8821 cctgaattga acgagattat cagaaatctt aatctggtca agaaacatat tcccgctaat
     8881 gattccgcta tggttatcag actcggctgg ggtagcggct ggaaacatat gaccggtgat
     8941 tatttagaaa gagataagga tattcttgaa cagattcgga tgaattttcg tcttggtcgc
     9001 cccggtatgc catttcctaa atctcgcaaa atcgctttcc agaatggcaa accgattttc
     9061 cctttcggct ggattaaatt agaggtgctg aattgaaagc gatttattta gccaatgtcg
     9121 gtaatcgaga tgtgaagttt gataatcaga acattaaagt gcctcgctta tccggtaaag
     9181 attatctaga ccggtttgaa aaagtcgctg agcatttaac atttccgatt atcactaagg
     9241 gactagaatg tgtattccaa gacttgcaag atataccttt agagcagtta atcctttttt
     9301 ataccgatca ggatcaaaat gaagtatccg agcaacatta tcaatcggac actctctatt
     9361 tcgccgatct tatcgaaaaa cttgttaatc ttagaatacc ggaaaaagtc aagaacgttg
     9421 tcaaaataaa aatatgtcac tctcctaatg actatgactt cacctatgag ttcttttcat
     9481 ctcgacttac tgaaatcgaa aacgaagtca agcccgaccg gatttatctt tccccggcag
     9541 gaggaattcc ggcttgcaat atgaatcttc tcctgcaagg cagtcgtatt tttacccgtc
     9601 gtgccgtaac gcttctgatt cctgaaaatc ccgataaacc ggccaaactt cagaaactcg
     9661 gggatctaat taatcgtgaa aataatcgct tgattatcca acgtctttta agtgaatatg
     9721 attttggtgg cgcagcggaa gtactggaaa atgaatccga tgataagtca aaatcgcttc
     9781 tggcgctggc tcgttcggtt caataccgac tctatttcga tttccaaacg gctttgaatt
     9841 acctgtctaa tgtgaatcct gaagttctac aaattgccga aactaatatc gccaattttg
     9901 gcaccgtcga aaatctcata aatactatcc aggaaaattt cacggcgctt atcaaaccaa
     9961 ttgaaccact ccatggtaat caaactgatt ttgatcaaaa aaataaatgg ctggaattgc
    10021 agaaaagact gaatgccgag gttctactga atgtcagaat caaatggctt tctaaacaat
    10081 atattgattt ccttggtcga atatttcgtc tgcaagaagg tgtcctgaga tggatttttg
    10141 aaaaggaaac cggatattca gccgactctg agcagtttcc caatgattat cctgattttc
    10201 ttaagcaaaa tcctgaattc aaagactatt tgaaaaatga atcaatcaat ggcaaaaatc
    10261 ctgttgctaa tcgcttaaat cggggtactt tgcttcttta tttacgattt ctcagtaaga
    10321 ttcgcaatga gtataagccg gttttgaaac aatgcgataa attggatcac ttatcagctc
    10381 tgcgcaataa aagcgccctg gctcatggca atgaaccggt aaatgattgc atcatttccc
    10441 agatatatgg taagaacgta ttggggaatc tttccaaatt ggcaagtaca tttgggcttg
    10501 aggaccactt gccccaaatt gattgtatca gaaatattat aatcgaaaat ttttgatttt
    10561 ttcgctcttt gacattgtga taaaaaaata gttctctgta agtgaatttt ttatgaaccg
    10621 tggcgaaggc atcggcggct taaggaggtc tctgctcaat tgactgggag ttatatttcc
    10681 tttttaataa tccgattttg aagaacttat tccgatagta gtttcagaag agtcttatct
    10741 aaaaaatagg gggtttgaaa atggctttta tctattttac tgaacaaggt tcaaaattac
    10801 tcaaatcagg taatcggcta atcgttgaaa aagacgggga aaagatagcc gatattccat
    10861 accggagtct taactgaaga tgctgatctg tatgtttttt gataaccgta accttaaaat
    10921 tgggtttaaa agacttaggc tacgaaaatt aatataccaa atttaatttc cttgtgttat
    10981 gaacacaata aaccgaaaaa aaagtataaa gaaaggctat ttaggattta tatttggact
    11041 ggttacgaaa ttactttgaa aaaagaattg aaaatcaggt ggttactaaa tgtgctgtga
    11101 taatcattga cccgatgata aggggattaa gactaccgat ttcgaagtta gaacttttgt
    11161 atcgttccgg cgtgataatc attgacccga tgataagggg attaagacga aagaaggaaa
    11221 gataatctcg gagatatact caccttgtga taatcattga cccgatgata aggggattaa
    11281 gacactaaat ttcttgccaa ccttcatcaa atgttctctt ggttgtgata atcattgacc
    11341 cgatgataag gggattaaga cgatagtagc tgagtttgaa ttgatccgtt aggataatgt
    11401 gataatcatt gacccgatga taaggggatt aagacataag gtaaactaaa tttatgccca
    11461 aattgtagag tgcgatgtga taatcattga cccgatgata aggggattaa gacagtattt
    11521 tgaatacttg gaccctatcg tgcattctat tggtgataat cattgacccg atgataaggg
    11581 gattaagacc aatagaactt tccacttgtt ttaaatttct tatcagaggt gataatcatt
    11641 gacccgatga taaggggatt aagacttagc ttgctcgatt tcatccttac tcattgcagc
    11701 tacgcgtgat aatcattgac ccgatgataa ggggattaag acgttgaggt agagtccatt
    11761 gttaagtttt ccctacgagg tgataatcat tgacccgatg ataaggggat taagacgttt
    11821 gaagaatagg ttaattttga aatacttatt ttatttctgt gataatcatt gacccgatga
    11881 taaggggatt aagactcgcc gccaaaaacg ccctcccgca cggcgaattt acggcctggt
    11941 gataatcatt gacccgatga taaggggatt aagacaatct catctatact ggcgattgag
    12001 tcaatcacgc caatggtgat aatcattgac ccgatgataa ggggattaag accttttgat
    12061 ttctctattt cggacttagt aatcgattct acggtgataa tcattgaccc gatgataagg
    12121 ggattaagac tagagttatg catcctgttg tgaaaatgat tcacaacgtg ataatcattg
    12181 acccgatgat aaggggatta agacccgaaa tagtccaaca taattactcc ttttttttca
    12241 attcgtgata atcattgacc cgatgataag gggattaaga cggtgcaggc ggtggcgtca
    12301 ccgtggatga tgtgcatatg tgataatcat tgacccgatg ataaggggat taagactacc
    12361 gatatacatt ttttatctca ttacctatta caacgtgata atcattgacc cgatgataag
    12421 gggattaaga ctataaaatc tgaacatcgt ttgcagtttt aggtttgggg tgataatcat
    12481 tgacccgatg ataaggggat taagactaaa aaatacgacc tctttacccg ccctatgctg
    12541 gcgtaggtga taatcattga cccgatgata aggggattaa gacatttagt aacataatcg
    12601 ggatgagctc cccaagtgtg cctttgtgat aatcattgac ccgatgataa ggggattaag
    12661 accgttcgat ttcagcatca atgtcctcag gtgtttcaaa cgtgataatc attgacccga
    12721 tgataagggg attaagactc gattgcataa gttatctgat cgttattcat tctcattgtg
    12781 ataatcattg acccgatgat aaggggatta agacttataa atattctttg agtcttagcg
    12841 tcttagcgag agaaatgata atcattgacc cgatatttag cccgcctcgt aggagtcggg
    12901 cagggggaat tatgacaaca ataaatcacc tttgcgcctt agcgtcttgg tggtatcatg
    12961 taataattaa tggcctgcta tttagcccgc cttggggaga ttctgggagg gagattaaga
    13021 caacactata aaaatcctta gcgtcctttg cggttaaaat tatcatgata atctttggtc
    13081 cggtaacgag cccttaatag agtcgggcag gggggattaa tctgagctca ggacacctga
    13141 aaggtagaga tagtgccatt gacgaactaa aattgttctg atctccggcc aataaaacat
    13201 tggaattaat gattgataat ggcgttgcgt tcttagcggt taaaagaaaa aaagtgttga
    13261 ccgatgaccc cgtgttaata tattattatg tggtatttac tcgaatttta aaatgtttta
    13321 cctctagaaa gtaattactt cagactatta tgtttttggt aagactttca gttctgctac
    13381 cacctagccc aaccaatttc cgccactcca aaggcggagc taaattggtt tcggtgcatc
    13441 cttcgtttta ctgaaatttg attttcagga acaacatttt aagacctgcg atctattttc
    13501 tcccaccact cggcgagaaa ggtaaggtag agaaaacagt tatcttattt cttgacaatc
    13561 ggagaataat ttattaaaat tattacaaat ggatagaaat ccgcaaaatc ggttaggaaa
    13621 ttggtttgtg gtggaattat cagtttttcg attaagaaac caccctctaa ggttacttta
    13681 gatagaaggt agttttatat ggaaagaatt ggttaaatct atgatatgta ataaagtgtg
    13741 attgaatatt atgcgtaaat cggttattgt tcttattctt ttatttctta ttaactgtga
    13801 aaaagatata aataatccaa ttatagagcc ggaaccagga atcggcaaaa caacgatatt
    13861 aatagaaaga ggactggttg attataattc ttacaatttt cctatagtaa gtcttaccta
    13921 cgcaaaaatt agtatatcgg atattgatac atccctatat tttgcaatgt cagaatcttc
    13981 tcctaatgat acaatcgtta ttctttctca cggtgtattt aatggtgagc taaatacaac
    14041 agggatacct atcgcagatg aatgtttgtt tatcaaagcc tatttcagaa atgaggatac
    14101 gagtgggact gtcttattat atgaaaatct ctggaattat tcgtttattg acagtgtaat
    14161 atttgagcca tataaactta ttggattgtc tgataccatt cggatcggta aacaacgtta
    14221 cttaagttgg aaagaaaatt atagaataga tacgttgaaa tttaatgttt attataagcc
    14281 taatatcaat atagtatatg atagattctc tttgtcaaca atggattttc tttataaaaa
    14341 ttcagcatac gattcggtaa aatatatagc caaattcatt gttggaatac cgtcaatgac
    14401 ttcagaaccc aatattgtat attatccata tagtacaata gaacttgttt atgagagtag
    14461 cattttaaaa gtaatatcaa gattaaacta tcaagttgaa tataaattac caacctggaa
    14521 gttaaaccac gttatagatt cgctggaaat ttgccgtaat aattggaaat cattatcaat
    14581 tcatgattat tcgatatatc agactattaa gtcctggtat ccctggtacg gtgacacctt
    14641 aaaaatcgcg gtctattcag atacaatcaa tgatattcag aaagtacctg atttatctac
    14701 tattgatcgt tcagtttggg aacgatataa aacgattgat gaactatttg aattagcggg
    14761 tttagattca actagatatg agctccgagt aaggtataac tcagattatt attatccaga
    14821 attcttatat tatgaattga aaccaccaat atataccgag ggaggatttg agtattatac
    14881 ctggaattta gaaatcagaa gactacttgt tagttgaaac tgctaaataa aataagcaca
    14941 taaccgttat ttagagttaa tatatgattt aaagtttgat gagtttatga aaagtatgag
    15001 aaaaaattgg attcatgaat cccataaatc cggcgcgact catagctacc taaatgtaaa
    15061 atggatgcgt tatagaaaat ttgcgcttat tatttattca ttctcgctaa taaactgcag
    15121 tccatttgag aataatttat ttcctttaga gggaagaata tttttcaaaa tatcggagga
    15181 ctataagcaa tacaaccgtg aaaataaccc tgaaatattt ttatttctgg aaactgagaa
    15241 aatatacggt tgctttaatt atggcatttc tacggattgt gatatagacg gtagaaaaat
    15301 tgaggtggat attttaggaa taaatcaacc gggagtttgt ttgacggcaa ttggaccggc
    15361 aatcgcgaga attcatctcc cattatctaa taagttatat tcattaaaga tatcaggcga
    15421 tgatttcact aataattatg ctgtgatagt tacagattca tcaatatcgg taacggggga
    15481 ctctaccgtc aatacgaagc cggaaataga attattctgg cgctatcctc ccaattcatt
    15541 tgtatatctg tatggtagca cttttgaaga ttcttctttg gcaaatgtat ttttggatac
    15601 tttgatgcaa gttatagata ttgctgaatt ttatttccct caatatggag aaatacctta
    15661 tccaaaatca tctcaaggtc attattggga tatgcccgca agatattttt attataattc
    15721 tgaagatgat tttaataaga ttgaagatat tttaagagac tttaaaagta tttatataca
    15781 ggataaacaa ggaatatctg taacaatacg aaattggcgt aataggaagt tccaaagttg
    15841 gttattataa aaagtatcta accatcagat gaaggcaacg aggtaagctc tgtgttatga
    15901 ttcacttgga aatgaaaaat tgttaagttt gaaataagag tcaaaaagaa aggaaaccaa
    15961 gatgaatcaa aatcgaatcc aacatgccgc cagtaaaaaa gtcgagattc tcagagaaca
    16021 cttagagaac caggtcccta tcagtgagat agcccgttgc tatggaataa atcccaactt
    16081 aatccataag tggaagaagc agttatttga agg
//
LOCUS       DAOSMP010000043        15665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_3764_length_15665_cov_22.075464, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000043 DAOSMP010000000
VERSION     DAOSMP010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 15665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15665
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3764_length_15665_cov_22.075464"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(543..3359)
                     /gene="uvrA"
                     /locus_tag="PKX09_07690"
     CDS             complement(543..3359)
                     /gene="uvrA"
                     /locus_tag="PKX09_07690"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOU17740.1"
                     /translation="MSTEHIVIKGAREHNLKNIDVKIPRDKFVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGLMEKPDVDFIEGLSPAISIEQKSASRNPRSTVGT
                     VTEIYDYLRLLYARIGIPYCYQCGKRIERQTTQQIVDNILSWPEDTRLMILAPLVRGR
                     KGEYREVFREVRREGFLRVRIDGKIYEVEHTPVLDKNKKHNIEVVIDRLSLNPTVKDR
                     LTESVELALKIGSGLLVVVINSENEQIFSEKLACTDCGISYEELEPRMFSFNSPYGAC
                     PTCDGLGTLMEIDPALVVPDETKTLSQGAIVPLGEQPRGGWYSAILKGLARKYNFDFV
                     TPFKKLPQEAKNALLYGSDGTKLSINLKSDRIDGEYHTPFEGVIPNLRRRYKQTTSTG
                     IRMWIQQFMSYRLCPQCQGARLKPESLAIKIGGFAINQLSAMSITKLREFFVNLELNP
                     TEQQIAHQILKEIHTRLNFLINVGVEYLTLDRMTHTLSGGEAQRIRLATQIGSQLMGV
                     LYILDEPSIGLHQRDNRRLIETLLHLRDNGNTVIVVEHDREMIESADYIIDLGPGAGA
                     TGGNVVFAGTPAEIKNEPRSLTGQYLSGAKTIPIPRNRRPGNAKQISLYGATGNNLKN
                     IDVCFPLNKFICITGVSGSGKSTLINETLFPILHRNFYGSQKVPLKYTDVQGLEYIDK
                     VIAIDQSPIGRTPRSNPATYTGTFTHIRDLFANLKESKIRGYTAGRFSFNVRGGRCEV
                     CGGDGIKKIEMHFLPDVYVLCEECKGLRYNRDTLEVKYKGKSIADVLNMSVSEAFEFF
                     RNIPSINKKLQTLIDVGLGYIKLGQQATTLSGGEAQRVKLSTELSKVGTGKTLYILDE
                     PTTGLHFDDIKMLLNVLNRLVDQGNTVIVIEHNLDVIKTADYIIDLGPEGGDNGGYIV
                     CEGTPEEVSAHPASQTGKFLAKIL"
     gene            3545..5170
                     /locus_tag="PKX09_07695"
     CDS             3545..5170
                     /locus_tag="PKX09_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOU17741.1"
                     /translation="MTQTGLDQETRTMILDTINSLKNELLTRERILELDDKSEFPLEI
                     VKELLSERIGLQMVFIPEEFGGLGGGALDTYYVSVELAKICLGVATAFLAIHLGAEPI
                     LLFGTEEQKQKWLAKIVNEAAIVAYAVTEADAGSNLDAIKTKAEPMTDESGNIVSYRL
                     NGSKQFISNGGYADFLTLLAKAPEGATFFIVDGKSAGLKRGKPEHKHGIRAANTSPLT
                     LENVIVPTENIVGGIPGVGLKQANDVFSYTRLMVAAFGLGAGLSAMEKAIQFAKTRIQ
                     FGTPLIEKQGYTHKLLVPNVVRLTAAQAYIEEIAKRVDSGDTDILVEGSIAKYFATEA
                     GNIAAEAAIQALGGYGYMHDYEVEKIKRDVRITMIYEGTSEIQQNIIYLYRWRTTVKS
                     KGEFYQKMAQEMRTIAEQSNLESAKIVAQALELLNDLIMFAHNQKLTRQQHIQFLLSD
                     VITWSEVAAALVRKTAEHLRDSAPDANQLRSVLRIFVAEMARLISNKGMEIILGSETV
                     SDETKKEWIEKVKNLNVVRAEYNIITEMDKILF"
     gene            5234..6784
                     /gene="gpmI"
                     /locus_tag="PKX09_07700"
     CDS             5234..6784
                     /gene="gpmI"
                     /locus_tag="PKX09_07700"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOU17742.1"
                     /translation="MRPVAIIIRDGWGYNENPRGNAVLAAKTPNIDSYKKNYPWTLLK
                     CSGEAVGLPEGYQGSSEVGHLNMGAGRIVIQELKRIDDGLSDGSLFESPKWKNLVANW
                     KANNSAFHLFGLLQDEGVHAHQEHLFKIMRRARQEFPEGEIIVHPFLDGRDTPPRSSL
                     EYIAKLNRVLAEVGHAKIGTIMGRYYGMDRSKNWNLTDTAYHCIVLAEGRPAPSAEAA
                     IRESYANDKTPDNVEMFDEYIPPYVIGGYTGMRDGDVILHTNYRQDRAIQLTRAFVDP
                     DYPGKLKAKPKVTYVGFTQYYDEFTEYLLGSMSSGGGMKNLLGEVISNAGLRQLRIAE
                     TQKFRHVTSFFNGKSTTPYPLEDQVEIKGRFDPATFASHPEMEAYNVTDELLRRLENN
                     PYTLIVVNYANGDMVGHTGVFEAAKKAIEIVDDNVGKIVRRLLELDAHILITADHGNS
                     EQMIDYETGMVKTSHTLFPVELIYVARDLAGKSLKSGGKLADIAPTALKLLGLEIPKD
                     MTADCLIS"
     gene            6862..8004
                     /locus_tag="PKX09_07705"
     CDS             6862..8004
                     /locus_tag="PKX09_07705"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HOU17743.1"
                     /translation="MKFSETFKSVVTRSTLKVFLVFLILIFSAAIFVFFFEKQNNPDE
                     YHNLWDAVWWVFVTIFTVGYGDIKPITPGGRLVAIFIMLVGVSMVSTITATISSIFVT
                     RKIREAQGLEAINYENHLIICGWNNRAESLINTILQLSGKKDMKLVLINELAENEINT
                     ILEKYSQFKIKFVYGDYSHPAPLERANIKRARTVLLLPNLHRYSPADADEKTILATLN
                     IKSSFPKVRVIAFIMNPENEAHVKRAKADEIVVSDKYADYFLAINILQPELPKVYDQI
                     LNPRMQNKIVVEAIPASFMNKPYRELFDYYHTQYKTLLLGLISEVESIGFSDFLSADT
                     SQLDAFIERKLRESGKSFGEENKVFVKLNPGDDYLIQKNEKAIVLR"
     gene            8001..8528
                     /locus_tag="PKX09_07710"
     CDS             8001..8528
                     /locus_tag="PKX09_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOU17744.1"
                     /translation="MNTKTKAQYVNISILSKYEIFNSLSEKNIEHFCEVIEVLEYESG
                     DTIIKEGEVGDSLLLLLDGTVEISQALTLKTDMSGIDTREKSLIKLSSTAHPFFGEMS
                     LFSDDDKRTATVKASTKCLVGRISRVDFFQICNSNPTIGNIVMQNIARVLTERLKQAN
                     QNVLKLTTALSLLIE"
     gene            complement(8709..9893)
                     /locus_tag="PKX09_07715"
     CDS             complement(8709..9893)
                     /locus_tag="PKX09_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17745.1"
                     /translation="MKKLKFDARDIFLTPRLALSGKKIWVHMVGLAAGWVIYTILAYV
                     ALLVNGQSLAEIWRMYHFFPILCCTTCQLPWYSWLIYGVGVVAWIIIFLLASVAVARI
                     TYKQLKGDEFFSSGDSFKYVKKHGVAAVMGPVSILMIIAFFVILAIIAAWLAKWPVLD
                     IIFLGIPYLLYFVVATFVVYTAVVFVVSLVLAPAIVGTAEEDTMETVFQSYSTLWAQP
                     WRLVLYEGIVGGLTVVATFIYGWALILGYRFFNFVFSASWLMDGKMSRIIEQASSYLF
                     GINSPLTPWISQFFNVYTPASLGIVKPLYLGTTEIITVVLITIALFIIAGTVVAYALS
                     NLSVGQALIYIILRKKKDDEDLIERKDEDELKEEEKSEEPASEETKAPESETPSSDEK
                     TE"
     gene            complement(9914..10678)
                     /locus_tag="PKX09_07720"
     CDS             complement(9914..10678)
                     /locus_tag="PKX09_07720"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOU17746.1"
                     /translation="MKKLSLTLTILLMLLSNCSKKPVVVTGVVGNPINLVPEIKKSED
                     TTGCTFKWGFIAKPAESIMDVLSFIPDSRSFSVSFIPDVPGDYELQFLSQDSNGQEKF
                     KQIIKCSILPDTAQSPSTDTVAGQMPPRDLSAPPPQYAQSYPGGENETTYSAMTQSQP
                     IKTGKNIQKIPGKYTIQLGAYKNYDKAEAELRKLRAYNLDTYIQKAYFKESNETWYRI
                     RTGTFDSYAEAKKVLNQLKARFPKENLWIDYVREDQ"
     gene            complement(10702..11118)
                     /gene="ndk"
                     /locus_tag="PKX09_07725"
     CDS             complement(10702..11118)
                     /gene="ndk"
                     /locus_tag="PKX09_07725"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOU17747.1"
                     /translation="MSMTLAILKPDCVKRKLIGEVISFIEKEGFRIVCLRMIHLQPQE
                     AAEFYSIHRGKDYFEGLMNFMTSGPSVLMVLERENAVEHFRKVIGATDPKKAAEGTLR
                     RKYAENVRYNVVHGSDSEENAKKEIAFFFSTMEIIK"
     gene            complement(11130..12071)
                     /locus_tag="PKX09_07730"
     CDS             complement(11130..12071)
                     /locus_tag="PKX09_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOU17748.1"
                     /translation="MNRTAVVALGGNAISPNNEKDTIANQFKNTRKSLQSVMNLIRQN
                     YHLALTHGNGPQVGNALLRVELTIDKAPFLPLGICVADTEGGMGYMIEQSLQNALIKA
                     QIPREVVSIITQVIVAADDPSIDNPTKFVGSWYSYNEIKELAARFGWQIKEDGQRGWR
                     RVVPSPIPRKIINSRAIKHLVDLGTIVIAAGGGGIPVYIMENGLYEGFDAVIDKDLAA
                     AVLAHDIAAQELYILTDIDSVKINFGKPNEESIDLIHIPELQCLLAEGQFPLGSMGPK
                     ISAAIAFIQSGGERVIITSLEKAIESLHGDSGTTIIA"
     gene            complement(12074..13426)
                     /locus_tag="PKX09_07735"
     CDS             complement(12074..13426)
                     /locus_tag="PKX09_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009217452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOU17749.1"
                     /translation="MNRRNIVIIGAAGRDFHNFNVYYRDNEQFNVVAFTAAQIPNIDN
                     RKYPSELAGKLYPKGIPIFAEEELTYLIEKLDVDECIFSYSDVPYQKVMALSALVNAA
                     GANFTLLGPKATMLKSTKPVISVCATRTGCGKSQTARKVIEILMAQGLKVVAVRHPMP
                     YGDLVAQKVQRFATLDDLKKYRCTIEEMEEYEPHIVRGNVVYAGVDYEAILRAAENDP
                     NGCDLIIWDGGNNDFPFFKPDLAITVVDPHRPGNELSYYPGEVNLRIADVIIINKIDT
                     ANPEGIQTVSQNIAIVNPQATVIYAASPVRVDHPEIIRDKRVLVIEDGPTLTHGEMQI
                     GAGIIAARQYGARECVDPRPFVVGKLAETFQKYPKIGPLLPAMGYSEDQIRDLEDTIA
                     RTDCDSVVIGTPIDLTRILKIKQPSARVYYELQEIGTPNLNEVLSDFCQRIKLNIQTK
                     "
     gene            complement(13834..14457)
                     /locus_tag="PKX09_07740"
     CDS             complement(13834..14457)
                     /locus_tag="PKX09_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOU17750.1"
                     /translation="MTLLDSYDIFRHKNMIKVALNKIIFYPPSKGYALLLKEVGGERQ
                     LPIIVGVFEAQSIALAIEEVKMPRPMTHDLFVNILHDLGAKILSVTVSDLIDGTFYAK
                     IEISLANSKKNLYIDARPSDAIALAIRMKAPIQVSEEVMQEAGQYLNSKRKDSPEKDD
                     SLPEIQNLDHLFELQNNLQSAIEEENYELAAKLRDQINRLKQELNIN"
     gene            complement(14487..>15665)
                     /locus_tag="PKX09_07745"
     CDS             complement(14487..>15665)
                     /locus_tag="PKX09_07745"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOU17751.1"
                     /translation="TRIGGMDSHSGILARAFGIPYLSNINEIDIIKKSQQIILDADRE
                     IVIVDAKPSVLKKYELKLRELEIRRQRLNETIPPAITRDGIKIEILLNAGFLSEVKNI
                     NLSLIDGIGLFRTEYLCLERDDLPNEEEQFRAYRQITMAMQGKPITFRTFDFGREKMM
                     AILNMEMFQKDAVFDTWGGIRFCLDNPRILITQLRALLRASQFGPLKIMFPLVSNSDE
                     ILQVLEIYQQVQSDLEKEGHAFNRNIPLGAMVETKSILDELEKLTELLNFFSVGTNDL
                     ALFLLGSKRADTISKNYYHPKIFQAINQIINVAQKKNFPVTVCGEMASDPYAILGLLA
                     LEVRSFSMSVSALIDINDLIRMVNIQKISSLKENLLTADNVQQVYKLLRSSYKRITKK
                     "
BASE COUNT         4404 a         3325 c         3400 g         4536 t
ORIGIN      
        1 aacaaaaccg gattttatca acaactttat atggatttat gaattaagca ggttaatagc
       61 gagctggact attttttgcc aaattacgat gtggttaact cttaactgat tcattacaga
      121 ataatatttg agtaagctct aaatcctcca gacccatgag gacaccgcga gccgagctta
      181 actgattcat tgcagaatgg tacttgatta atcttgattg gcaatttttg cttcagaaca
      241 ggtatttaaa cgtaaatgtg ttttataata aaaaagaaaa gctggaatga aacatctggg
      301 cagagttcat tccagctact tttgggcatt ttcctgagtg ggcgacatca ttgtgagggt
      361 acgcctaacc atctggggta gtatggtttc aaagaacact aattaatagg acaataattg
      421 tgccaatagg attatattaa tattatcaag tagttataaa tatttatgat ctgctagaat
      481 cgtggcgaat gggacactga attgtgttta tcccaccacg aaacgagctc atttcaggga
      541 gcttatagga ttttggctaa gaatttcccg gtttgggagg cagggtgggc tgatacttct
      601 tcaggagtgc cttcgcagac aatatatccg ccgttatcac cgccctctgg gcctaaatca
      661 atgatataat cggcagtttt tattacgtct agattatgtt cgatgacgat gacagtattg
      721 ccttgatcta ccaaccgatt caaaacattg agcagcatct taatatcatc gaaatgcagg
      781 ccggtagtag gttcatcaag gatgtacaga gttttgccgg tgcctacttt gctcaattca
      841 gttgataatt ttacccgctg ggcttcgccg cctgataatg tcgtagcctg ttggcccagt
      901 ttgatataac ccaagccgac gtctattaga gtctggagtt ttttattgat agaaggaata
      961 tttctaaaaa attcaaaggc ttcacttacg gacatattca aaacgtcggc aatacttttc
     1021 cctttgtatt tgacctcgag agtatcgcgg ttataacgca gccccttgca ttcttcacaa
     1081 agaacataaa cgtccggcaa aaagtgcatt tcaattttct taattccgtc accgccgcaa
     1141 acttcacaac gaccgccccg aacgttgaat gaaaagcgac cggcggtgta gccgcggatt
     1201 tttgattcct ttaggtttgc aaataggtcg cggatatgag taaaagtacc agtataagtt
     1261 gccgggttgg agcgcggtgt tcgtccgatc ggcgattgat ctatagcgat tactttgtca
     1321 atatattcca gcccttgaac atcggtatat ttcagtggta ctttctggga accatagaag
     1381 ttgcggtgca aaattggaaa taatgtttcg ttgattaggg tacttttgcc tgaaccagat
     1441 acaccggtaa tgcaaatgaa tttatttaaa gggaaacaca catcgatgtt tttcaaatta
     1501 ttgccggtgg cgccatataa actgatttgc ttggcgttgc cggggcggcg atttcgtggt
     1561 attgggattg ttttagctcc ggagagatat tgaccggtca gtgagcgcgg ctcattttta
     1621 atctcagccg gagttccagc gaataccaca ttgccgccag tagcgccggc tcccggtccg
     1681 agatcaatta tataatccgc cgattcgatc atttctcggt cgtgttccac cacaattacc
     1741 gtattcccat tatcccgcag atgaagaaga gtttcaatta atcggcggtt atccctttgg
     1801 tgcaaaccga tgctgggttc atcgagaata tacagcacgc ccattaattg cgaaccaatt
     1861 tgagttgcca atcgaatacg ttgagcttcg ccgccagaaa gggtgtgggt catacgatcc
     1921 agagttaggt actctacccc aacattaatt aaaaaattga gcctagtgtg aatctctttt
     1981 aaaatttgat gagcaatttg ttgttcggtc gggttgagtt cgagattaac gaaaaattct
     2041 ctcaacttgg taattgacat agcgctcaac tgattgatcg caaatccccc gatttttatt
     2101 gccagactct ccggttttaa acgggctccc tgacattggg ggcataagcg ataactcata
     2161 aattgctgaa tccacatgcg aattccggtg gaagtcgttt gcttatagcg acggcgtaaa
     2221 ttaggaatta ctccttcgaa aggcgtatga tactcgccgt caattcgatc cgatttcaaa
     2281 ttaatggaaa gtttggtacc atcggaacca tataataaag cattcttagc ttcctgaggt
     2341 aattttttaa atggtgtaac aaaatcgaaa ttatatttgc gcgccagtcc ctttaatatt
     2401 gctgagtacc agccgccgcg gggctgttcg cccaatggca cgatggcgcc ctggctgagc
     2461 gttttggtct catccggtac aactaatgcc gggtctattt ccatcaaagt tccgagacca
     2521 tcacaagtgg gacaagctcc atacggacta ttgaaggaaa acatacgagg ttccaattcc
     2581 tcgtagctga ttccgcagtc ggtacaggct aatttttcac tgaaaatctg ttcattttcg
     2641 ctattgatca ccactactag taaaccagaa ccgattttaa gcgccagttc gaccgactca
     2701 gtcagacgat ctttgacagt aggattaaga ctgaggcgat caatgaccac ttctatattg
     2761 tgttttttat tcttatcaag aacgggcgtg tgctcgactt catagatttt cccgtcaatt
     2821 cgaactctta ggaagccttc gcgacgaact tcgcgaaaca cttctcgata ttcaccttta
     2881 cgtccacgca ccaagggcgc taaaatcatc aggcgggtgt cttcaggcca gctaagtata
     2941 ttatcaacaa tttgctgagt agtttgacgt tcaatacgtt taccgcattg gtagcaatac
     3001 ggaattccga tgcgagcata cagcagacgc agataatcat aaatttcagt caccgttcct
     3061 acggttgaac gcggattgcg agacgccgat ttctgttcaa tagaaatggc tggcgaaagc
     3121 ccttcgatga agtccacatc cggtttttcc attaatccca agaattggcg ggcataggct
     3181 gatagcgatt cgacataacg tcgctgacct tcggcatata ttgtatcaaa agccagtgaa
     3241 gacttgcccg aacctgatag accggtaatg accacgaatt tgtcacgtgg gatttttacg
     3301 tcaatatttt tcagattgtg ttcgcgggcg cccttgatga cgatatgctc tgtactcatc
     3361 agactttcca tttaaattat ccgattaaat tatcacctta ttaccataat gaaaaatatt
     3421 tttcacgaat tttctacaaa tttattttcc gttttagtaa taaagtttca cactttttct
     3481 ttcctcaatc actattgact gattatattt ctatgttagt ttaaatcaat gtaggagaag
     3541 ttaaatgacc cagactggcc tcgatcagga aactcggacg atgatcttgg acactattaa
     3601 ttcactgaaa aatgaattgt tgactcgcga aaggatcctc gaactcgatg ataaaagcga
     3661 attcccgctg gaaatcgtca aggaattgct gagtgaacgc atcggtttgc agatggtctt
     3721 cattcctgaa gaattcggcg ggctgggcgg cggcgcactc gatacttatt acgtttcggt
     3781 ggaactggca aaaatttgcc ttggcgttgc cacggctttt ctggcaattc atcttggtgc
     3841 cgaaccgatt cttttgtttg gaaccgaaga gcagaagcag aagtggctcg ctaaaattgt
     3901 caacgaagcc gcaattgtag cttatgccgt aactgaggct gatgccggta gcaatctcga
     3961 cgcaatcaag accaaagccg aaccgatgac cgacgaatct gggaatatcg tatcttatcg
     4021 tctaaacggt agtaaacaat tcatctccaa cggtgggtat gccgattttt taactttact
     4081 ggcaaaggct ccggaaggcg cgactttttt catagttgat ggcaaatccg cgggattgaa
     4141 acgcggcaaa ccggaacata aacatggcat ccgggcggca aatacttcgc ccctcactct
     4201 ggaaaatgta atagtgccga ctgaaaatat cgtcggtgga atacccggcg ttggattgaa
     4261 acaggctaat gatgtctttt cctatacgcg gctgatggtg gcggcattcg gactcggagc
     4321 tgggctttcg gctatggaaa aagccatcca gttcgccaaa acgcgaattc agtttgggac
     4381 gccattgatt gaaaagcaag gctatacgca taaattgctg gtacctaatg tcgtccgttt
     4441 gactgcggcg caggcatata tcgaggaaat tgccaagcgg gtggattcgg gcgatactga
     4501 tattctggtc gaaggttcga ttgctaaata ttttgcgacg gaagccggaa atatagccgc
     4561 ggaagccgct attcaagctc tgggtggcta tggttatatg cacgattatg aagttgagaa
     4621 gatcaaacgc gacgtgcgca tcactatgat ttatgaaggt accagtgaaa tccagcaaaa
     4681 tattatctac ttgtatcgct ggcgaacaac cgttaaatcc aaaggcgaat tttatcaaaa
     4741 aatggctcaa gagatgcgaa ctattgcgga acaatcgaac ctggaatctg ccaaaattgt
     4801 ggctcaagcg ctggagcttc tgaatgacct gatcatgttc gcccataatc agaaattaac
     4861 tcgccagcaa cacattcaat tcttgctttc ggatgttatc acctggtcgg aagttgccgc
     4921 ggctttggta cgcaaaaccg ccgaacattt acgtgattcg gcaccggatg ctaatcaact
     4981 ccgttccgtt ctgcgaattt ttgtagcgga aatggcaaga cttatctcta acaaaggtat
     5041 ggaaattatc ctgggtagcg aaactgtaag cgatgaaacg aagaaagaat ggatagaaaa
     5101 agtaaaaaat ctgaatgtgg ttcgcgccga gtataatatc ataaccgaaa tggataaaat
     5161 cttattttaa acaatcaatt aaaacaaccc ttcgagcctt cgagccttgg tggttaaaaa
     5221 atcagaggta aaaatgcgac cggtagcaat tattattcgt gatggttggg gttacaatga
     5281 aaacccgaga ggcaatgccg tcttggcggc taaaacgccc aatattgatt cctataaaaa
     5341 aaattatccc tggacattgc taaaatgttc gggcgaagca gtcggattgc ccgagggtta
     5401 tcaagggagt agcgaagtcg gtcacctgaa tatgggggct gggcggattg taattcagga
     5461 acttaaacgc attgacgacg gactttccga tgggtcgtta tttgaatcgc ccaaatggaa
     5521 aaatctggta gccaactgga aagccaacaa ttcggccttt catctttttg ggcttcttca
     5581 ggatgaaggc gttcacgctc accaggaaca tcttttcaag attatgcgtc gggcgcggca
     5641 ggaatttcct gagggcgaaa ttatcgtgca cccttttctt gatggtcgtg atacaccgcc
     5701 gcgcagttcg ctggaatata ttgccaagct taatcgcgtt ttagcagaag tcgggcatgc
     5761 gaaaattggt acgattatgg ggcgttacta cggtatggac cgctccaaaa attggaattt
     5821 aaccgacact gcctatcact gcatcgttct tgcggaaggg cgaccggccc cgagtgccga
     5881 agccgctatc agagaatcct atgccaatga caagacaccc gataatgtcg aaatgttcga
     5941 tgaatatatt ccaccgtatg ttatcggcgg ttataccgga atgcgagacg gtgatgttat
     6001 actgcacact aattaccggc aagatcgcgc cattcagctt acgcgagcat tcgttgatcc
     6061 ggactatccc ggtaaattaa aagccaagcc aaaagtcact tatgtcggtt ttactcaata
     6121 ttatgatgaa tttaccgagt acctgctcgg gtcaatgagt tccggtggcg gaatgaaaaa
     6181 tctcttagga gaggtgattt ccaacgccgg tttacgtcaa ttacgtattg ccgaaaccca
     6241 gaaatttcgg cacgttacca gctttttcaa cgggaagtcc acgacaccct atccgctgga
     6301 agaccaggtg gaaatcaaag gtcgcttcga tccggcaact tttgccagcc accctgaaat
     6361 ggaagcttac aacgtaaccg acgaattact gcgacgcttg gaaaataatc cctatacttt
     6421 gattgtcgtt aattatgcca atggtgatat ggtcggacat actggagttt tcgaggcggc
     6481 taaaaaagcc attgagattg ttgatgataa tgtcggtaaa attgtccgac gtctcttaga
     6541 gctggacgcg catattctaa ttaccgctga tcacggtaac tccgaacaga tgatagacta
     6601 cgaaaccggt atggttaaga ccagccatac tctctttccg gttgaattga tttacgttgc
     6661 ccgcgattta gccggaaaat cactcaagtc tggcggtaaa ttggcggaca ttgcgcctac
     6721 tgctctgaaa ttactcggtc tggaaattcc gaaagatatg acggctgatt gtttgattag
     6781 ctaactacaa agttcaagat tgctggtgct aaaggatagc gaacgaaata ttggcagtta
     6841 gaattgttaa tctgagataa tgtgaaattt tctgaaacct tcaaatctgt cgttacccga
     6901 tcaaccctaa aagtattttt ggtttttcta atcttaattt tttcagcggc gattttcgta
     6961 tttttcttcg aaaaacaaaa taaccctgat gaatatcaca atctctggga cgcggtttgg
     7021 tgggtttttg taacaatttt cacggttggt tacggcgaca ttaagccaat tactccagga
     7081 ggaaggcttg tcgcaatttt cataatgcta gtaggcgttt cgatggtttc gaccatcacc
     7141 gcgacgatct cttcaatttt tgtaacacga aaaattaggg aggcacaagg tttggaagct
     7201 attaattatg agaatcatct cattatttgc ggctggaata atcgcgccga atcattaatc
     7261 aatactattt tacaattgtc cggcaaaaag gatatgaaat tagtattaat caatgaactg
     7321 gccgaaaatg aaattaacac gatcctcgag aaatattccc aatttaaaat caaatttgtc
     7381 tatggagatt actcccatcc ggcacccctc gaacgggcga acatcaagcg cgccaggaca
     7441 gttttgcttc tacccaatct ccatcgctat tcgccagccg atgccgatga aaaaactatt
     7501 ctggcaacct tgaatataaa gtcgtcgttt ccgaaggtca gagtcattgc tttcattatg
     7561 aatcccgaaa atgaagcgca tgtcaaacgg gctaaagccg acgagatcgt agttagtgat
     7621 aaatacgctg attattttct ggcgattaat attttacaac cggaattacc gaaggtttac
     7681 gaccagattt taaatccgcg catgcagaat aaaattgtgg tcgaagctat tccagccagt
     7741 tttatgaata aaccttatcg ggaattattc gattactacc atactcaata taagactctc
     7801 ttgcttggct taatttctga agtcgagtca atcggatttt ccgatttcct ttcggcggat
     7861 acttctcaac tcgacgcttt cattgagcgt aaattacgtg aatccggcaa gagtttcggc
     7921 gaagaaaata aggtctttgt caaactaaat ccaggagacg attatctaat ccaaaaaaat
     7981 gaaaaggcga ttgttctccg atgaatacca agactaaagc ccaatatgta aatatttcca
     8041 ttttatcaaa atatgagatt ttcaatagtc tttccgaaaa aaacattgag catttttgcg
     8101 aggtaattga ggttctcgaa tacgaatccg gcgatacgat catcaaggaa ggcgaggtcg
     8161 gcgattcact tcttctactt ctggatggta ctgtcgagat ttcgcaagct ttgactttga
     8221 aaaccgatat gtccgggata gatactcgtg aaaaaagctt gataaaactt tccagcaccg
     8281 cccatccatt tttcggggaa atgagccttt ttagcgatga cgacaaacgc acagccactg
     8341 tcaaagccag cactaaatgc ctggtcggtc gtattagcag ggtagatttc ttccagatat
     8401 gcaactcaaa tccgacgatc ggcaatattg taatgcaaaa tattgcccgt gtcctgaccg
     8461 agcgcttgaa gcaagccaat cagaacgttc tgaaactgac cacggcgctc agtctgctaa
     8521 tcgaatagtg aatgtatttt ttctcttgac aaaattttta aaattggcaa agtatccact
     8581 atttgctcag ttggggttcc gatggaactt ttatagaaaa tctatcccga tatcaggcta
     8641 tataaaccaa acaaaaaggg cgtcagaatt cggacgccct ttttatcaaa accaacgaaa
     8701 agactttgct actctgtctt ttcgtctgaa gaaggagttt cagattccgg agcttttgtt
     8761 tcctcactcg caggctcctc ggatttttct tcctctttta actcgtcttc atctttccgt
     8821 tctatgagat cctcatcatc ttttttctta cgtagtataa tgtaaataag ggcttgtcca
     8881 acactgaggt tagaaagggc ataagcaacg accgttccgg caattataaa caaggcgatc
     8941 gttatcaaaa ccacggttat aatttcggtt gttccaagat aaagcggttt tactatacct
     9001 agcgaagctg gagtataaac attaaaaaat tggctaatcc agggagttaa tggagaattg
     9061 atgccgaaca ggtaagagga agcctgttcg attatgcggg acattttacc atccattagc
     9121 cagctagcgc taaatacgaa gttaaaaaat cgatagccaa ggattaaagc ccagccataa
     9181 ataaaagtag ccactaccgt tagtcctccg acaatccctt cgtataaaac caatcgccag
     9241 ggctgtgccc aaagtgtgga gtaactttga aaaacggtct ccatagtgtc ttcttcagct
     9301 gtaccgacga tcgctggagc cagaaccagc gatacaacaa agaccaccgc ggtgtatacg
     9361 acaaaagtcg ctacgacaaa atagagcaga tacggtatcc ccagaaaaat aatatcaagg
     9421 acaggccatt tagcaagcca agcggctatt atcgctagaa ttacgaaaaa agcaattatc
     9481 attaaaatag aaaccggacc cattacagcg gcgacaccgt gttttttgac atacttgaaa
     9541 ctgtcgcctg atgagaaaaa ctcatcacct tttaattgtt tgtaagtaat tcgtgctaca
     9601 gctacagatg ccagtaagaa aattattatc caggcgacca cccccactcc gtaaattaac
     9661 caactatacc agggtaattg acaggtggta cagcatagaa ttgggaaaaa gtgatacatc
     9721 ctccagattt ctgccaggct ttggccattg actagcaaag ctacatatgc aagtatggtg
     9781 tagattaccc agccagcagc taatccgacc atatgaaccc agattttctt gccactcagt
     9841 gccaaacgag gagtcaagaa aatatccctt gcatcaaatt tcagtttttt catttataac
     9901 ctcctacaat tgtttattga tcttcacgaa cataatctat ccagagattc tctttgggaa
     9961 aacgcgcttt tagctggttc aaaacttttt tggcttcagc gtaagaatca aaggttccgg
    10021 tgcggatgcg ataccaagtt tcgttagatt ccttaaaata cgccttttgt atataagtat
    10081 ccagattata cgctcttaat tttcttaatt cagcctcagc cttatcatag tttttgtaag
    10141 cgcccagctg gattgtatat ttcccaggaa ttttctgaat gttttttccg gttttgatcg
    10201 gttgggattg ggtcatagct gaataggtcg tttcgttttc cccgcccgga taggattgag
    10261 cgtattgagg gggaggtgct gacaaatctc gcggtggcat ctgaccagca acggtgtctg
    10321 tactagggga ttgagcagta tcgggcaata tgctacactt tataatttgt ttgaattttt
    10381 cttggccgtt gctgtcctga ctgagaaact gcaattcata atcgccggga acatccggta
    10441 taaaagaaac tgaaaaactg cgactatccg ggataaacga gagaacatcc ataatactct
    10501 cagccggttt ggcaataaaa ccccatttaa aagtgcatcc agtagtgtct tccgactttt
    10561 tgatctccgg aactaaattg atgggattgc cgacgacacc tgttacgacc accggttttt
    10621 tgctacaatt gctcagaagc atcagtagaa tcgttaaggt caaagatagt tttttcataa
    10681 atctggccct ccgcatgtta atcatttgat aatttccatc gtagaaaaga aaaatgcaat
    10741 ttctttcttt gcattttctt cagaatctga gccgtggaca acgttatatc gtacattttc
    10801 ggcatatttt cgacgtaagg ttccttcagc ggctttcttt ggatcagttg ctccaataac
    10861 tttgcgaaaa tgttccacag cattttcgcg ctcgagaacc attaaaacag atggcccgga
    10921 ggtcataaaa ttcattaaac cttcgaaata gtccttcccg cggtgaatac tatagaattc
    10981 agccgcttct tgcggttgaa gatgaatcat ccgcaggcaa acaatcctaa agccttcttt
    11041 ttcgatgaaa ctaatcacct cgccgatcaa tttacgttta acacaatccg gtttaagaat
    11101 agcaagtgtc attgacatat ttttcctttc tatgcgatga tggttgtacc gctatcacca
    11161 tgcaggcttt caattgcttt ttcgagggag gtgatgatta ctctttcacc gccagattgg
    11221 ataaaggcga tagcggcgct gattttaggt cccatcgagc ctaatggaaa ttggccctct
    11281 gccagcaagc attgcaattc ggggatatgg atcaaatcaa ttgattcttc attgggtttg
    11341 ccaaaattga ttttgacact atcaatatcg gtcagaatat acagctcctg agccgcgata
    11401 tcgtgagcca agacagccgc cgccaggtct ttatctataa cggcatcaaa accttcgtat
    11461 agtccatttt ccattatata aaccggaatg ccacctcctc ctgccgcaat cacaatggtg
    11521 cctaaatcaa ccaaatgttt aatagcacgg ctgttaataa ttttgcgggg gatcggcgaa
    11581 ggcactactc ggcgccatcc gcgttgaccg tcctctttaa tttgccagcc gaaacgtgcc
    11641 gccagttctt tgatttcatt ataggaatac caacttccga cgaacttagt tgggttgtcg
    11701 attgaaggat catctgccgc tacgatcacc tgggtaataa ttgaaaccac ctcccggggt
    11761 atctgcgcct tgataagggc attctggagg gattgttcga tcatatagcc cataccgcct
    11821 tcggtatccg ctacgcaaat tcccagcggt aagaatgggg ctttgtcaat cgttagttcg
    11881 acccgcagaa gggcattgcc gacctgtggc ccattgccgt gagtcaatgc cagatgataa
    11941 ttctggcgaa ttagattcat caccgattgc agacttttgc gcgtgttttt aaactggtta
    12001 gcaattgtgt ccttctcatt attaggtgag atggcattgc cgcctaatgc tacaaccgcg
    12061 gtccgattca tatctacttt gtttgaatat taagtttaat tctttgacag aaatcggata
    12121 atacttcgtt taaattggga gtgccgattt cctgtaattc gtagtaaacc cgagcagaag
    12181 gctgtttgat tttaagaatg cgagtcagat caattggtgt tccgataaca actgaatcgc
    12241 aatcagtccg cgcaatcgta tcttccagat cgcgaatttg gtcttcacta taacccatag
    12301 ctggtaaaag aggaccgatt ttcggatatt tttggaatgt ctcagccagt tttccaacaa
    12361 cgaacggacg gggatcaaca cattcccggg cgccgtattg acgagccgct attatgccag
    12421 cgccgatttg catttcccca tgggttaacg tcggaccgtc ttcgattact aacactcgtt
    12481 tatcgcggat aatttccgga tggtcaactc taacgggcga agccgcataa ataaccgttg
    12541 cctgaggatt aactatggct atattttggc tcacagtttg aatcccttcc ggattagcgg
    12601 tatcaatttt attgatgatg ataacgtcag cgatgcgtaa gttgacttca ccgggataat
    12661 aactaagttc attaccgggt cggtggggat caacgaccgt gattgccagg tcgggtttaa
    12721 aaaaaggaaa atcgttattg ccgccgtccc agatgatcag gtcgcatccg ttgggatcgt
    12781 tttccgccgc tcgcaaaatc gcctcgtaat caactccggc atagacgaca ttaccccgca
    12841 cgatatgcgg ttcatattct tccatttctt cgatggtgca acggtacttt tttaagtcgt
    12901 ctaaagtggc aaagcgctgg actttttggg caaccagatc accgtagggc atcggatgac
    12961 gcacggctac caccttcaaa ccctgcgcca ttaaaatttc aatcaccttg cgtgctgtct
    13021 gacttttccc gcaaccggtg cgagtggcac agactgaaat caccggtttg gtgcttttta
    13081 acatagttgc ttttggtccg agtaaggtga aattcgctcc cgccgcattg acgagcgctg
    13141 aaagcgccat aactttctga tagggcacat cactatacga aaagatgcat tcgtcaacgt
    13201 cgagtttttc aattaggtag gtcagttctt cctcagcgaa aattggaatg ccctttggat
    13261 aaagttttcc agccaattcg gatggatact tgcggttgtc aatatttggg atttgagccg
    13321 ccgtgaaggc gacgacatta aattgttcat tgtcacgata gtaaacattg aaattatgaa
    13381 aatcgcgacc agccgcgccg ataataacaa tattcctgcg attcatcatt taattccttt
    13441 cattgattaa ttggttttaa aaaatttact gaaaatgaag gcgcaggaaa tttatttata
    13501 gccaaaatga taggtccaga gtatcatggc atttttataa ctatctgttc tttcgaccga
    13561 gccggcggca gatacatcgt gagttatagg gagcaaaatg attttcactt aataaagtta
    13621 ggtaaattta tgtcgaattt caaggggcgg cgacttgctt cgtcaagtta aaggcctgcc
    13681 agattaaaat tgcttaaaaa ataattctca gaatttccaa taaaatggga atttggaatt
    13741 atttctaata ttttttaatg gatttatgag taaatgctgc gactgcttcc ttattataaa
    13801 gaaaggtagt aaatcacatt cttttattga tttttaattg atgtttaatt cttgtttaag
    13861 gcgattgatc tgatcgcgta gcttagcggc taattcgtaa ttttcttctt cgatggcgct
    13921 ttggagatta ttctgcaatt cgaagagatg atccagattc tgtatttctg gaagggaatc
    13981 gtctttttca ggagaatctt tacgtttgga gttcagatat tgtccagctt cctgcattac
    14041 ttcctcactt acttgaatag gtgctttcat gcgaattgcc aaggcaatag cgtcgctcgg
    14101 tcgagcgtct atatataaat ttttcttgct attagccagg ctgatttcaa tcttggcgta
    14161 aaaagttcca tctatcaaat cactgacagt cactgataat attttagcgc ctaaatcgtg
    14221 taaaatatta acgaataaat catgagtcat tgggcgcggc attttcactt cctcgatagc
    14281 gagtgctatg gattgggctt caaaaacacc tactataatc gggagctgac gttcgccgcc
    14341 gacttctttt aaaaggaggg catagccttt gctaggcggg taaaaaatga tcttattgag
    14401 tgcgactttg atcatattct tatgtctgaa tatatcataa ctgtcaagca gagtcaagta
    14461 ttaaaataag tttcccctat tacatcttat ttttttgtaa tacgtttata ggagcttcgt
    14521 aaaagtttat aaacttgttg aacgttatca gcggtgagca gattttcctt tagtgaactg
    14581 atcttctgaa tatttaccat tcggattaag tcattaatgt caatcaaggc gctgacgctc
    14641 atactgaaag agcgtacctc caaagccaat aaaccgagga tagcgtaggg atcggaagcc
    14701 atttcaccac aaactgtgac tggaaagttc tttttttgag caacatttat tatttgatta
    14761 atggcttgga aaattttggg gtgatagtag ttcttggaga tggtatcggc tcgttttgaa
    14821 ccgagtagaa aaagtgccag gtcattggtg ccgacgctaa agaagttcag caattcagtt
    14881 agtttctcca gttcgtccaa aatactttta gtttcgacca tagcaccgag aggaatgttt
    14941 ctattaaagg catgtccttc tttttctaaa tcggattgaa cttgctggta gatttcaagt
    15001 acctgtaaga tttcatcgga attggaaacc agcggaaaca taattttcag cgggccgaac
    15061 tggctggcac ggagcaaagc ccgcagttgg gtgattaaaa ttcgcgggtt gtctaaacag
    15121 aaacgaattc caccccaggt gtcaaagacc gcatcttttt gaaacatttc catattgaga
    15181 atagccatca ttttctcgcg accaaaatcg aaagtgcgaa aggtaattgg tttgccctgc
    15241 atagccattg ttatctggcg ataagcccta aattgctctt cctcattagg gagatcgtca
    15301 cgttcaagac atagatattc cgttcggaat agacctattc cgtcaatcag gctcagattg
    15361 atatttttaa cttcagagag gaatccggcg ttcagcagga tttcgatttt gattccgtca
    15421 cgggtgatgg ctgggggtat ggtttcattt agacgctgac gacgaatttc taattcccga
    15481 agttttaatt cgtacttctt aagcacggac ggtttggcat cgacaataac tatttcacgg
    15541 tcggcgtcca gaattatctg ttgacttttc ttgataatat caatttcgtt aatattggat
    15601 aggtagggaa ttccaaatgc tcgggctaga attcccgaat gtgaatccat cccaccgata
    15661 cgagt
//
LOCUS       DAOSMP010000044        15372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_3868_length_15372_cov_16.574133, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000044 DAOSMP010000000
VERSION     DAOSMP010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 15372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15372
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3868_length_15372_cov_16.574133"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PKX09_07750"
     CDS             <1..672
                     /locus_tag="PKX09_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase"
                     /protein_id="HOU17752.1"
                     /translation="ETARINEVKMKPVGLTEWNIFAVGSKQQVSYINGIHAVLVLGEL
                     IKNKYGLACRWNLANNWDNGNDHGMFSQGGEPNVPRWNPRPVFYYMTYFQQFCGDHLV
                     NTNVVGDSDVVAYASSFSSGEAGIILVNKGNSPKSARLEFPNFQPGRRCYWYLLTGGK
                     DNGDFSLQVFINGVGPELPAGGSANFEKIKAYSARITKPLVFDLPPYSVLYLLLESDR
                     KIPVK"
     gene            730..>1204
                     /locus_tag="PKX09_07755"
     CDS             730..>1204
                     /locus_tag="PKX09_07755"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOU17753.1"
                     /translation="MFEKIKIVPKILLMTISLGLFLACLPTCGQRIRLPERGSVSWLP
                     GSKWEEALLTGNGTIGAMVIGRPHDETIILNHALLYLPASDRLPPINQASRLDEIRQL
                     LLQGKYAEAAKIPVEIGETEGYQGTHWTDPYMPALNIRLNMKAANVRDYARAVDFT"
     assembly_gap    1205..1304
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1305..3326
                     /locus_tag="PKX09_07760"
     CDS             <1305..3326
                     /locus_tag="PKX09_07760"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOU17754.1"
                     /translation="LNMKAANVRDYARAVDFTIGETMVFWLDEGGRFERKLFVSRADN
                     CIVMSIKGSGKINTSLEFAMHPVEWSQQEMVNGAIEFTEIGAKDCWLTYRCKFRRHYD
                     YNPQGYEAVGRVILKGGNLSSEGNQLVIQNADEILLLVRLEPNFDWENSRVSELQSAL
                     QNQPTTYKTLLERHVNIHGNLYNRVKLDLGGRKLKTLQSEVLVARAREEVTPPMIEKF
                     FDAARYNILCATGTRPPNLQGIWNGTWSPPWSSDFTNDGNAEVAISSLLCGNLPELML
                     AYFDYHEKLLPFYEDNARRLYGCRGIHIPSRTSSHGWDIHFGETWCLTFWTAGAGWVS
                     SFFYDYFLYTGDTTFLKERAYPFMKAAAQFYEDFLTIGENGKYVFNPSYSPENNPANS
                     NSQACINATMDVMVAKQLLRNCIQAAKILKVDESQAIKWQEMLALMPEYEINEDGALR
                     EWLWPGLEDNYNHRHVSHLYGLYEMLDPDIAGNPELIKSAKNAIAERMKIRRRENGGA
                     MVFGLVQMALVAANLGDAEMAEELINWMASYYWSNSLASLHDPGNLFNMDLSGGFPAA
                     VIRTLVYSEPGMIKLLPALPNSWPAGSISGVLVRGQIEIKELKWRENQILVTLQSRVN
                     QNLELRMPRPVGRILGPDRDLITRLPNDDRNFQIFLPVDSDVTVKFVYQ"
     gene            3413..5374
                     /locus_tag="PKX09_07765"
     CDS             3413..5374
                     /locus_tag="PKX09_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HOU17755.1"
                     /translation="MISSKIIRSFVTLLISLTMIQTCSSNKPKNQYLKSPDGNITVRF
                     EISPAGKIFYRVAYRNKEIVQASRMGIVREDADFSESLKLDSVSASRPINFNYQMPQG
                     KKSHYAYNGNEKRFYLTNHVNQKIDVVFRASNDGVAFRYIFPGQSDEPKTILREISEF
                     HFSPRTKAFIQPMAMAKSGWCQTNPSYEEIYQQNVAIEQLPFSEPGWVFPALFEDGDF
                     WMLISETAPDRNYCGCRLQQDSTSHIFYIDFPSDLETFPGGIWLPTSPLPWSTPWRII
                     TIGKGLKNIVESTLGTDLAEPARLKEIDYVKPGRSSWSWVLLKDDSTVYEVQKRFIDY
                     AAEMNWEYCLIDADWDWKIGYDKVAELAAYAQTKGVGILLWYNSSGDWNSTTYSPKSK
                     LLTHNDRLREFARLKKMGIRGIKVDFFGGDGQSMMTYYQDIFEDAAQFGLVVNCHGAT
                     LPRGWQRTYPNLLTMEAVRGFEFITFSQADADLEPTHACLLPFTRNVFDPMDFTPVCF
                     SEIPNIRRITTNGFELATAVIFCSGIQHFAEIPAGMERVPDYVKNFMQEVPVSWDETC
                     FIDGFPGKFVVLARKSGQTWYLAGINGADVARQLKLDCHFLEGDRTGQLITDAADGRS
                     FMTKEIHISPQSPLSIKLEKHGGFIIKFD"
     gene            5508..7268
                     /locus_tag="PKX09_07770"
     CDS             5508..7268
                     /locus_tag="PKX09_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU17756.1"
                     /translation="MHRRMVTLILTLMLIVGCSEDKSAADISIDSAEITGESVVPPGP
                     EWLHNAVFYQIYPQTFYDSNGDGIGDLNGIIEKLDYVQSLGVDGFWINPFFVSPFNDA
                     GYDISDYYQVAPRYGTNQDARRLFLEAEKHGLKVLFDYVISYTSIEHPWFQESARQEP
                     NKYSNWYVWTNNTWIDPPEAYRDAFIKGYSRRNGQFMRNFYWSQPALNFGFVKPESDW
                     MLPIDHPDVLALQEELQKVLRFWMDMGADGFRADMAGALVKNANIVESDPEYANRDEG
                     TKIFWQKIRKIIDEEYPQAFMVAEWSYPVAALDGGGFHADFFHWFPGYNDLLQKESWR
                     IMNGHSEGHSFFDREGKGDITQFLASYLEQYRQTVGKGYINLPLGNHDLSRLNIERSD
                     DDLEIIFAFSLTMPGIPFIYYGNEIGMRQLYDLPNVEGAYPPRAGARTPMQWSQGKNL
                     GFSTANPEKLYLPVDPAADAPNVADQQDDPNSLLNRVKNLIQLRKNSKALRAYAEFVP
                     VYAKKGEYPFIYARACEDETVLVILNPANKAATARFRLKPVFSQFELLAGTESELKLE
                     DKYYQLTIPGGSYSIYQLGN"
     gene            7332..9386
                     /locus_tag="PKX09_07775"
     CDS             7332..9386
                     /locus_tag="PKX09_07775"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOU17757.1"
                     /translation="MGSFLRKISGLTIAPILLALSSNAFSAGLPTLRFEHLSTQDGLS
                     HNSIIEIIQDRQGFMWFGTLNGLDRYDGYNFTAYRPILGDTTSLCHNVINALYEDRRG
                     ILWIGTLDGLNRYDRARDCFIRIRHNPNNPNSLVDNRIRCLTEDNYGQIWIGTEGGLS
                     CYDPQSGTFKNYLPDPNNPYSLGDFIIHDIYNDGRDQLWIATEKEGVYRFNRITQSFT
                     RYRHETTESPKAGTSAVITIGEDRWGNLWIGTWGDGLQRFDVNAQRFRKVPIFCKGKK
                     PFTTKIITKLLIDQSDRMWMSTYDAGLFVIPQIPRNVTTAGIEGIQHYLCEPLDETSI
                     QSNALWTVYLDRTGVVWIGNENGGINKCDTKQSHILHYRFGLSGANSLSNNHVTSFFE
                     DQYGKIWIGTRFGGLNRFDPVTNHFRVYRKDEQNNGLSSDAILCLTGNRDYLWIGTDG
                     SGLDRYDFKSGQFKHFRHDDQNPSSLGENAVYALYLDKSNTLWVGTWGGGLSKLNSDG
                     QTFSQYPIDRINDRLNVVTSIAEDTSGGLWLGTYGKGLVFFNPNNEKMLFFRPDDTDS
                     LSLGHENINTLFVSQDGTLWIGTMGGGLNYVQYFQADSGIAHFGRYDQRHGLPDNIIE
                     SLIEDGDGFIWMGTNHGISRLNPANGEIHNFDRNDGFGQDNFYRESVLRASNGMLYFG
                     GI"
     gene            9425..9961
                     /locus_tag="PKX09_07780"
     CDS             9425..9961
                     /locus_tag="PKX09_07780"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17758.1"
                     /translation="MGGQIRLVKQDLGQSVIPKISTIPDTTEYVVKNTLTPLAFDFGT
                     QFKTGFKSLVFGMSIRNFSTDIKYVEEPFQLPLVFNLGISMDLFDLISQINQNQSLYM
                     SIDASHYRSRAEQVKIGLDWQLLKVLSLRCGYITNEDESGLTFGIGVSKFGFTFDYAY
                     TPYGVFNQVQRLTVRMSF"
     gene            9984..11174
                     /locus_tag="PKX09_07785"
     CDS             9984..11174
                     /locus_tag="PKX09_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17759.1"
                     /translation="MKSNRFLIISALCLAVMKCEYDVTQPKWYQDYTEPASPAITNIE
                     PDSAVAGCNYINIIGENFSGTPTVYFDNVTAEVISNLGNSIQVRRPNLASDGCLVKVA
                     CDSALVVAQSTEPYKITQVVQKYGNFVENKELNAVAVDSAENVYVIDYTNIIYKVTPA
                     GDKTTMLDKAKRTTYDATLGPDGKLYLLGNNRSIDVLDPQSGKVAEWIKLKAGKVVKF
                     GDFDKNGYLYTGGRSTGLVVVAPDLTPNYTSLYEKDEIKGIRVFGNYVYVSLVCTTPD
                     LQNPALAIWRNPIDNSGNPGEKELVLDLTAYKNYSDDALNSFSLSSDGKLFLGINAQT
                     PLLAIDLNTTSADYFYLGIIAPYCKSFSWGRGNYLYMICGDTALRVEWTLYRIDMGVT
                     STRE"
     gene            complement(11247..>11524)
                     /locus_tag="PKX09_07790"
     CDS             complement(11247..>11524)
                     /locus_tag="PKX09_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5110 domain-containing protein"
                     /protein_id="HOU17760.1"
                     /translation="YRGADGSFTLYEDENDNYNYEKGAYSTINFYWDDTKNELTIGEP
                     NGSFPGMLIERQLQIVLVSPNHGAGIEITPRPDKIIKYQGKAQTTRF"
     assembly_gap    11525..11624
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11625..14013)
                     /locus_tag="PKX09_07795"
     CDS             complement(<11625..14013)
                     /locus_tag="PKX09_07795"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOU17761.1"
                     /translation="MKSQITYSLMILALAVSQCGQSGKVKPIQNGVDLKTRDLNVRVQ
                     FYADDIARVIKWRPESSAEKLSLMVIRDSLPDLSITVEENDNDIILKSERMTIRLVKN
                     DGQLEYFTTSGTSILKENKKAVFTPVTLPYEKAFNIQQNFKLTPDEGLYGLGQHQDGY
                     MNYRGRTVKLVQTNTDAVTPFLISTRGWGILWDNYSKTIFQDTAETMSLWSEVGDNLD
                     YYLIYGGTMDSVISGYRYLTGKAPLYGKWAYGYWQSKEHYKNRDELLSVVNEYRRRRI
                     PIDNIIQDWNYWDGNENWSQMFFDPKKYPEPEEMIRQIHNQNFHIMISIWPGLGPNTA
                     IYQEMEQNGFLYNTVGWANFKYFDAFNPAANDLYWKYIKNGLISVGIDALWIDSTEPD
                     IVNATTKESQEYEMKKVGRNYLGSWARYLNAYSLVMMDKLYQNQRQDSDRKRVYMLTR
                     SVFAGQQRTGATTWSGDIGASWEIYQNQISAGLNFCMAGIPYWTFDIGGFVIGAYGGV
                     FINGGQDPAYQELYTRMFQFGTFCPIFRAHGSETPREIWCFGEFSPHLIKFNHLRYRL
                     MPYIYSLAWRVTNDDYTMMRGLPMDFPNDPETYSLGDQYMFGPAIMVCPVTEYMLHRP
                     PGKSILISGDHFKTNDGKAGLKVQYFRDPDFEILTHEQIEPDINHLWYTGRPDYMTDS
                     AFSVRWEGKLIPSQTGLHQFHLKSYDAKRIFINGKELPIVYTSVEQYTDLINLNAGQG
                     YDFRLEMQNRSTGAAKMQLNWKTPAIFAQEKIVETRPQTRNVYLPAGTQWIDFWTG"
     gene            14429..>15372
                     /gene="ispG"
                     /locus_tag="PKX09_07800"
                     /gene_synonym="gcpE"
     CDS             14429..>15372
                     /gene="ispG"
                     /locus_tag="PKX09_07800"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOU17762.1"
                     /translation="MNNFKRKITRTVYVGNVPVGGGFPIPVQSMTTAKTHDLKAILPQ
                     IEKLEAAGCQIIRVTVPDEQALKALPEIKKRMTVPLVADIHFDYRLALGAIDAGADKI
                     RINPGNIGGAERVKVVLAKARAAHIPIRIGVNSGSLEKDLRAKYGGATPEAMLESARR
                     HLEICEENDFRDVIVALKSSEASLMIAANRLFSEKYDYPLHLGVTEAGPVWSGTIKSA
                     IGIGTLLAEGIGDTLRVSLTADPVEEVRAGFEILKALNLTKSGIRLVSCPTCGRTSVN
                     LIEIVNEVESKIRDLPQNLTVAVMGCEVNGPGEARSADL"
BASE COUNT         4159 a         3125 c         3545 g         4343 t
ORIGIN      
        1 gagaccgccc gaataaatga agtaaaaatg aaaccggtgg gcttgaccga gtggaatatt
       61 tttgccgtcg ggtcgaaaca gcaggtcagc tatattaatg gcattcatgc cgtcctggtg
      121 ctgggtgaat taattaagaa taaatatggc ctggcttgcc gctggaatct ggcgaataac
      181 tgggataatg gcaatgatca cgggatgttt tcgcagggtg gtgaaccgaa tgtgccgcgc
      241 tggaatcccc gaccggtttt ttattatatg acttactttc agcaattttg tggcgatcat
      301 ctggtcaata caaatgtcgt cggtgatagc gatgtggttg cctacgcttc gagcttcagt
      361 tccggcgaag ctgggataat tctggtcaat aagggaaatt ctcccaaatc agcgcgactg
      421 gaatttccca attttcaacc cgggcggcgc tgctactggt atctgctgac tggcggcaag
      481 gacaacggtg atttttcact gcaagttttc ataaatggtg ttggcccaga attgccagct
      541 ggcggttcag ccaattttga gaaaatcaaa gcctattcag cccgaatcac caagccactg
      601 gttttcgatt tgcctcccta ttctgttcta tatctgttac tggaaagcga ccgaaaaatc
      661 ccggtaaaat aatgctatct atctgtaaac accttagatt ttttaccatt aatcaaaaat
      721 agaaatacta tgttcgagaa gatcaaaatc gtgccaaaaa ttttgctaat gacaatttcg
      781 ctgggattat ttttagcctg tttaccgacc tgtggacagc gcatcaggtt accggaacgc
      841 ggctctgtca gctggctacc gggaagcaaa tgggaagagg cgcttctcac cggcaatggg
      901 acgatcgggg caatggtcat cgggcggccg catgatgaaa cgattatttt aaatcatgct
      961 ttactctatc tcccggcgag tgatcgctta ccgccgatta accaagccag ccgattggac
     1021 gaaatccgtc aacttttatt gcagggaaaa tatgcagaag ccgccaagat tcccgtcgaa
     1081 atcggggaga cagaaggtta tcagggaact cactggactg acccctatat gccggcgttg
     1141 aatattcgct tgaatatgaa agccgccaac gtccgtgact atgctcgggc agtggatttt
     1201 acgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttgaat atgaaagccg
     1321 ccaacgtccg tgactatgct cgggcagtgg attttacgat cggcgaaacg atggttttct
     1381 ggcttgatga aggcggtcgg tttgaacgca aattgttcgt ttcgcgagcc gacaattgca
     1441 tagtgatgtc aatcaaaggt agcggtaaaa tcaacaccag cctggaattt gctatgcacc
     1501 cagtcgaatg gagtcaacag gaaatggtca atggtgctat tgagtttacc gaaattggag
     1561 caaaggactg ttggctaact taccgctgta agttcagacg ccattatgat tacaatccgc
     1621 agggttatga agccgtcgga agagtgattc tgaaaggcgg aaatttatcc agtgaaggaa
     1681 accagctggt cattcaaaat gccgatgaaa ttctgctttt ggtgcggttg gaaccgaatt
     1741 ttgattggga aaattcccga gttagtgagt tgcaatcagc tttacaaaat cagcccacga
     1801 cttacaaaac actcctcgaa cgtcatgtga atattcacgg caatttatat aatcgggtaa
     1861 aactcgattt gggcggtcgc aaactaaaaa cactgcaatc cgaagttctg gttgccagag
     1921 cccgtgaaga agttactccg cctatgatcg agaaattctt cgatgccgcg cgctataata
     1981 ttctttgcgc tactggaacg cgcccgccga atttacaggg aatttggaat ggcacttggt
     2041 cgcctccctg gtcatcggat tttacgaatg atggcaatgc cgaggtggct atttccagtt
     2101 tgctatgtgg caatttgccc gaattgatgc ttgcttattt cgattatcac gaaaaattat
     2161 tgccgtttta tgaagataac gcccgccgtt tatatggttg tcgcggaatt catattccgt
     2221 cccgaaccag ttcgcacggc tgggatattc actttggcga aacctggtgt ttgactttct
     2281 ggacggctgg ggcgggttgg gtctccagtt ttttctacga ttatttttta tatacgggag
     2341 atacgacttt tttgaaagag cgggcttacc cgtttatgaa agcggcggcg caattttatg
     2401 aagattttct gactatagga gagaatggca aatatgtttt caatccgtcc tactccccgg
     2461 agaataatcc ggctaattcc aattcacaag cttgcatcaa tgccacaatg gatgtaatgg
     2521 ttgctaaaca gcttctgcgc aattgcattc aagccgccaa aattcttaag gttgatgaat
     2581 cccaagcaat taaatggcaa gaaatgctag ccctaatgcc ggagtatgag atcaatgaag
     2641 acggcgctct ccgcgaatgg ttatggcccg ggctggaaga caattacaac catcggcacg
     2701 tttcgcatct ttatggatta tatgaaatgc ttgatcctga tattgccggt aatcctgaac
     2761 ttataaaatc tgcaaaaaat gcgatagccg aacggatgaa aatcagacgg cgggaaaatg
     2821 gcggcgcaat ggtctttggc ttggtgcaaa tggcattggt tgccgccaat ttgggtgacg
     2881 ctgagatggc ggaggaactg atcaactgga tggcgagcta ttactggagc aattcattgg
     2941 cgagtctgca tgatccgggc aatttattca atatggattt aagcggcggt ttcccagccg
     3001 ccgtcattcg cacgctggtc tattctgagc ccggaatgat caaattactg ccggctttgc
     3061 ctaattcctg gcctgccgga agtattagtg gtgttctggt gcgtggacaa atcgaaataa
     3121 aagaactgaa atggcgggag aatcagatat tagtgacttt acaatcgcgg gtgaatcaaa
     3181 atttagaatt gaggatgccg cgaccggttg ggcggattct tggcccagat cgagacttaa
     3241 taactcgatt gccgaatgat gatagaaact ttcagatctt tctgccggtt gacagtgatg
     3301 tcaccgttaa atttgtttat caatgaatct tacagttgat cggaatctga atttctaaat
     3361 tgtaaaatag cgggaaactt tataattgaa aatatttaat atgaggagaa aaatgattag
     3421 ctcgaaaatc attcgttcgt ttgtgacatt attaatttca ttaacgatga ttcaaacttg
     3481 tagctctaac aagccgaaaa atcaatatct gaaaagtcca gatggcaata ttacggttcg
     3541 gtttgaaata tcgccggctg ggaaaatttt ctatcgcgtt gcttatcgga acaaggaaat
     3601 tgttcaagct tcccgaatgg gtattgttcg agaagatgct gattttagcg aaagtctaaa
     3661 gttagattcg gtctcggcct cccgcccgat taatttcaat tatcaaatgc cacaaggcaa
     3721 aaaaagccat tatgcttaca acggcaatga aaagagattt tatctgacaa accacgtgaa
     3781 tcaaaaaata gatgtggttt ttcgcgcctc gaatgacggg gtagcctttc gttacatttt
     3841 tcccgggcaa tcggatgaac ccaaaaccat cttaagggag atcagcgaat ttcatttttc
     3901 acctcgaact aaagctttca ttcaaccgat ggcaatggct aaatcgggct ggtgccagac
     3961 caatccttcc tatgaggaaa tttatcagca aaatgtcgcg atcgagcaat tgccatttag
     4021 cgaaccgggc tgggtttttc cagcgctctt cgaggatggc gatttctgga tgctgattag
     4081 tgaaactgcg cccgatcgca attactgcgg atgccggttg caacaggatt cgacgagtca
     4141 tattttttat attgattttc catcggattt ggaaactttt ccgggtggga tttggttacc
     4201 gacttcacct ctaccatgga gcaccccctg gcggattatt acgatcggca aagggttgaa
     4261 aaatattgtc gaatcaactc tcggaacgga cctggccgaa ccggctcgat tgaaagagat
     4321 tgactatgtc aaaccgggtc gctcgtcgtg gagctgggtg cttcttaaag atgattccac
     4381 agtttacgaa gtgcaaaaaa ggtttattga ttatgccgct gaaatgaatt gggaatattg
     4441 tttgatagat gccgattggg attggaaaat cggttatgat aaagtcgctg agctggcggc
     4501 ttacgcccaa accaaaggcg tcggaattct gctctggtat aattcttcgg gcgattggaa
     4561 tagcacaact tacagcccga aaagtaaatt gctgactcat aatgatcgcc ttagagaatt
     4621 cgcacgcctc aaaaagatgg gaatacgcgg gattaaagtg gatttcttcg gtggtgatgg
     4681 gcaatcaatg atgacctact atcaggacat ttttgaagat gcggcgcagt tcggtctggt
     4741 ggtgaattgt catggcgcta ccttaccacg cggttggcag agaacgtatc ccaatctgct
     4801 gacgatggag gcggtgcgcg gttttgaatt tataactttt agtcaagctg atgccgatct
     4861 ggaaccgacc catgcttgcc ttttgccttt tacacgcaac gtttttgatc caatggattt
     4921 tacaccggtt tgtttttcgg aaataccaaa tattcgacgg ataaccacga atggctttga
     4981 attggctact gcggttatat tctgttcggg aattcagcat tttgcggaaa tcccggctgg
     5041 tatggagcgt gtcccggatt atgtcaaaaa ttttatgcag gaggttccgg ttagctggga
     5101 tgagacttgt tttattgacg gttttccggg caaatttgta gtgctggcaa gaaagtcagg
     5161 gcaaacctgg tatttggctg gaataaatgg cgccgatgtc gcaagacagt tgaaattaga
     5221 ttgtcacttt ttggagggag atcgtactgg tcaactaatt accgatgcgg ccgacgggcg
     5281 ttcttttatg acgaaagaaa ttcatatttc tcctcaaagc cctttatcta ttaagctgga
     5341 aaaacacggt ggttttataa taaaatttga ttgataacca gataaatcct aatattgaac
     5401 tgtttttcaa agggaatatt attggcagat ttctttttaa ttgatggtaa aagagcaggt
     5461 gaaagtacgg agagtttgat caactcctga aatgatcgga ggaatttatg catcgtcgga
     5521 tggtgacact tattttgacc ttaatgctaa tcgtaggatg tagtgaagat aagtccgcag
     5581 cggatatttc tatagattca gcggaaataa ccggagaatc agttgtgccg cccggacctg
     5641 aatggttgca taatgccgtg ttttatcaaa tttatccgca gacattttac gattccaacg
     5701 gcgacggcat tggtgatttg aatgggatta ttgaaaaact ggactatgtg cagagtctcg
     5761 gcgttgacgg attctggatt aatccttttt tcgtatcacc ttttaatgac gccgggtatg
     5821 atatttccga ttattaccaa gtggcgccgc gttacggtac caatcaggat gcccggcgtt
     5881 tatttttgga agccgaaaaa cacgggctaa aggtcctttt cgattatgtc atcagctaca
     5941 ccagcatcga gcacccctgg tttcaagaat ctgctcgtca ggaacccaat aaatattcga
     6001 actggtacgt ctggaccaat aatacctgga ttgatccgcc ggaagcctat cgcgatgctt
     6061 ttattaaagg ctatagccga cggaatggcc aatttatgcg caatttttac tggagtcagc
     6121 cagctttgaa tttcggattc gtcaaaccgg agagcgattg gatgttaccg attgaccatc
     6181 cggatgtgct ggcacttcag gaggaattgc agaaggtttt gcgtttctgg atggatatgg
     6241 gtgcggacgg ttttcgagcc gatatggcgg gggcattggt caagaatgcc aatatcgtcg
     6301 aaagtgatcc tgaatatgcc aatcgtgacg aaggcacaaa aattttctgg cagaaaatca
     6361 gaaagatcat agacgaagaa tatcctcaag cctttatggt agcagaatgg tcatatccgg
     6421 tggcggctct ggatggaggt ggtttccacg ccgatttttt tcactggttt ccgggttaca
     6481 acgatctact gcagaaagag agttggcgaa ttatgaatgg ccattccgaa gggcatagtt
     6541 tttttgatcg agaaggcaag ggtgatatta ctcaattttt ggcaagttat ttagagcaat
     6601 atcgccagac agttggcaag ggctacatta atctgccact gggaaatcac gacctatcgc
     6661 ggttaaatat cgaacgttct gacgatgatc tggagatcat ttttgctttt tcactgacga
     6721 tgcctgggat tccatttatc tattatggca atgaaattgg aatgcgccag ctttatgatt
     6781 taccgaatgt cgagggcgcc tatccaccgc gagctggggc aaggactcca atgcaatggt
     6841 ctcaaggtaa aaatttgggc ttttctaccg ctaatccaga aaaattatac ctgccggttg
     6901 atcctgccgc tgatgcaccc aatgtagccg atcaacagga tgatcctaat tcattgttaa
     6961 atcgagtaaa aaacctcatc caattacgca agaattcgaa agccttacgc gcctatgccg
     7021 aatttgtacc ggtttatgcc aaaaaaggag aatacccttt tatttacgcc cgggcttgtg
     7081 aggacgaaac tgtgctggtt atattaaatc cggctaataa agccgcgacc gcccgattcc
     7141 gtctcaaacc ggtgttttct cagtttgaat tattagctgg aacggagtca gaactcaagt
     7201 tggaagacaa atattaccag ctgacaattc cgggaggcag ttattctatt tatcagttgg
     7261 ggaactaaaa tggagttaat ttcccgagat taaattgaat taataaatga tcaccatggt
     7321 cattgaatct tatgggtagt tttctgagaa aaatatcagg attgacaatt gcgccaattc
     7381 tgctggcttt aagtagcaat gctttcagtg ccggattacc gactttgcgt tttgagcatc
     7441 tttctactca agacggttta tcccacaatt ccattatcga gatcattcag gatcggcagg
     7501 gatttatgtg gtttggaact ctgaatggtt tggatagata cgacggatat aatttcacgg
     7561 cttaccgacc aattttagga gatacgacca gcctttgcca taatgtaatc aacgccttat
     7621 atgaagatag gcgtggaatt ttgtggattg gcactctcga cggcttgaat cgttatgatc
     7681 gggcgcggga ttgttttatt agaattcgtc ataatcctaa taatcccaat tcgttagttg
     7741 ataatcgcat acgttgtctt accgaggata attacgggca gatctggatt ggcacggagg
     7801 gcgggctaag ttgttatgat ccgcaatctg gcacttttaa gaactattta cccgacccga
     7861 acaatcctta cagcttaggt gattttatca ttcatgatat ctataatgat ggtcgcgatc
     7921 agctctggat tgcgaccgaa aaagaaggtg tttatcgctt taatcgcata actcaatcgt
     7981 ttactcgtta tcgtcacgaa actactgaat cacccaaggc cggcaccagc gccgttatta
     8041 ctatcggcga ggatcgatgg ggaaacctct ggattggcac ctggggtgac ggactgcaac
     8101 gatttgatgt caatgcacaa agattccgca aagtacccat tttttgcaaa ggtaaaaaac
     8161 cgtttactac taaaataatc acaaaattat taattgatca atcagaccgg atgtggatgt
     8221 ctacttatga cgccggatta ttcgtcatac ctcaaatccc gagaaatgtt accaccgccg
     8281 gcattgaagg aatccaacat tatctttgtg aacctcttga cgaaacgagc atccaatcca
     8341 atgcgctctg gacggtttat ctggatcgaa ctggtgtggt ttggatcgga aatgaaaatg
     8401 gcggaatcaa taaatgcgat acgaaacaga gtcatatttt gcattaccgt tttggacttt
     8461 ctggggctaa tagtctctcg aataatcatg ttacctcatt ttttgaagat caatatggga
     8521 aaatttggat cggcactcgt ttcggaggtc tgaatcgctt cgatccggtt acgaatcact
     8581 ttcgagttta ccgaaaagat gaacagaata atggtctcag ttcggatgcg attttatgtt
     8641 tgactggcaa tagagattat ctctggattg gtactgacgg tagtggtctg gatcggtacg
     8701 atttcaaaag cggccagttt aagcatttcc ggcatgatga tcaaaatccc tcctctcttg
     8761 gagaaaatgc cgtttacgct ctctatctgg ataaatctaa taccttatgg gtaggcacct
     8821 ggggcggcgg tctatctaaa ttgaattctg atgggcagac ttttagccaa tatcctatag
     8881 atcggattaa tgaccgtctg aacgtggtca cttctattgc cgaagatacg tccggtggac
     8941 tctggctggg aacctatggt aaagggctgg tattcttcaa tccgaacaat gaaaaaatgc
     9001 tgtttttccg ccctgatgat accgattcgc tgagtctggg acatgagaat atcaatactt
     9061 tgtttgtcag ccaggatggg actctgtgga ttggtacaat gggaggtggg ttgaattatg
     9121 tccaatattt tcaagcggat tcgggcattg cgcactttgg gcggtatgat caacgccatg
     9181 gtctgcccga taacatcata gagagtctta ttgaagatgg agacggtttc atctggatgg
     9241 gaaccaatca tggtatttct cgtctgaatc ctgccaatgg tgaaattcac aattttgatc
     9301 gcaatgatgg attcgggcag gacaattttt atcgtgaatc ggttttacgc gccagcaacg
     9361 gtatgcttta ttttggtgga atataggact gggttatgcg aagcgcttga cggagtggtt
     9421 cagtgtgggc ggtcaaatcc gcctggtcaa acaggattta ggccaaagtg tgataccgaa
     9481 aatttctacc attccggaca ctaccgagta tgtagtcaaa aacacactga ctccgctggc
     9541 tttcgatttc gggacgcagt tcaaaaccgg ttttaaaagc ttggtcttcg ggatgtccat
     9601 tagaaatttc tccaccgaca ttaaatatgt cgaagaaccg tttcaactac ctttggtttt
     9661 taatctggga atttcgatgg atttattcga cctaataagt caaattaacc aaaatcaatc
     9721 actgtatatg agcattgatg ccagtcatta tcgctcccgc gctgagcaag tgaaaatcgg
     9781 tctagattgg caactattga aagtgttgtc attacggtgc ggttatatca ctaacgaaga
     9841 tgagagcggt ttgacttttg gaatcggcgt atccaaattc ggttttacgt ttgactatgc
     9901 ttatacgccg tatggtgttt tcaatcaggt acagcgtttg acagtacgaa tgtcattttg
     9961 agcgatagaa ggagaaaagt atcatgaaat ctaatcgctt tttaataatc tcagccctct
    10021 gtttggcagt aatgaaatgc gaatatgatg ttactcaacc taaatggtat caggattata
    10081 ctgaaccagc atccccagct attactaata ttgagccgga cagtgctgta gccggttgca
    10141 attatattaa cattattggt gaaaatttca gtgggactcc aacggtctat tttgataatg
    10201 taacagcgga agtaatctcc aatttgggca attctatcca ggttcgtcgt cccaatctgg
    10261 caagtgatgg ttgtctggtt aaggtggctt gcgatagcgc tcttgtggtc gcgcaaagca
    10321 ctgagcctta taaaattact caggttgtcc aaaaatatgg taatttcgtc gaaaataaag
    10381 aattaaatgc cgtagcggtt gatagtgctg aaaatgtata tgtaattgat tacaccaata
    10441 ttatttacaa agtaactcca gctggtgaca aaaccaccat gcttgataag gctaaacgca
    10501 caacctatga tgctaccctt ggaccagatg gaaaattgta tcttttgggt aacaaccgct
    10561 caattgacgt tttagatcca caaagcggca aggttgctga gtggattaag cttaaggcag
    10621 gcaaggtggt aaaatttggt gattttgata agaatggtta tttatatacc ggaggtcgga
    10681 gcacaggact ggttgtcgtt gctccagatc ttacgccgaa ttatactagt ttgtatgaaa
    10741 aagatgaaat caaaggtatt cgcgtgtttg gtaattatgt gtatgtaagc ctggtttgta
    10801 ccacccccga cttgcaaaat ccggctctgg caatttggcg taatccaatt gataatagtg
    10861 gcaatccagg agaaaaagaa ttagtgcttg atttgacggc ctataaaaac tattctgatg
    10921 atgctttaaa tagcttttcg ctttcatctg acggcaaatt atttctgggt attaacgcgc
    10981 agactcctct tcttgccatt gatcttaaca ccacgagcgc cgattatttt tatctaggta
    11041 taatagctcc ctattgtaaa tcttttagct gggggagggg aaattaccta tatatgattt
    11101 gtggcgatac ggctcttagg gtagagtgga cactctatcg cattgatatg ggagtcacca
    11161 gcactcggga ataatctgtc tgtcactcga ttaatgacaa acggccggct ttttgaccgg
    11221 ccgttttgtt tttatgaaga atgaaatcag aacctggtgg tttgcgcttt gccttgatat
    11281 ttgatgattt tatccggtcg cggggtaatt tcgattccgg cgccatggtt gggtgaaaca
    11341 agcactatct gcaattgacg ttctatcaac atacctggaa aagaaccgtt aggttctcca
    11401 attgtcaatt catttttggt atcatcccaa taaaaattaa tggtcgaata ggcacctttt
    11461 tcataattat aattatcgtt ttcatcctca tagagggtaa aactaccatc ggctccccga
    11521 taaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttccag tccagaaatc
    11641 aatccactgc gttccggcag gtaaataaac attgcgggtt tggggtcgcg tctcgactat
    11701 tttctcttgg gcaaagattg cgggggtttt ccaatttaac tgcatcttgg cggctccggt
    11761 cgaacgattt tgcatttcga ggcgaaaatc atatccctgt ccagcattta ggttgatcaa
    11821 gtcagtatat tgctcgacgc tggtataaac tatcggtaac tccttgccat tgataaaaat
    11881 gcgtttagcg tcgtagcttt tcaagtgaaa ttgatgcagg ccggtctgac tggggatcaa
    11941 tttaccttcc cagcgtactg aaaaagcgga atcggtcata taatccggtc ggccggtgta
    12001 ccagagatga ttaatatcgg gctcaatctg ttcgtgagtc aaaatttcaa aatccggatc
    12061 acgaaaatat tgaactttta agcctgcttt tccatcatta gtcttaaaat ggtcaccgct
    12121 gattaaaatg ctttttccag gtggacgatg caacatatac tccgttaccg ggcagaccat
    12181 aatagccgga ccgaacatat attggtcgcc gagagagtaa gtctcaggat cgttcgggaa
    12241 atccatcggc aaaccgcgca tcatagtgta atcatcatta gtcacccgcc aggctaagga
    12301 ataaatataa ggcataagcc gatagcggag atggttaaat tttattaggt gcgggctaaa
    12361 ttcaccaaag caccagattt cccgtggcgt ttcactcccg tgcgcccgga aaattggaca
    12421 gaaagttcca aattgaaaca tacgagtata aagttcttga taagccggat cctgaccgcc
    12481 attaatgaat actccaccat aagcgccaat gacgaatcca ccgatatcaa aagtccaata
    12541 aggaatgcca gccatacaaa aattcagacc ggcactaatt tgattttgat aaatctccca
    12601 gctggcgcca atgtcgcctg accaagttgt ggcaccggtt ctctgctgac cagcaaagac
    12661 ggaccgggtc agcatatata ctcttttgcg gtcggaatcc tgacgctgat tctggtaaag
    12721 tttatccatc attaccaaag aatatgcgtt cagataacgc gcccaactgc cgaggtaatt
    12781 ccgacctacc tttttcattt cgtattcttg ggattctttg gtggtggcat tgacgatatc
    12841 cggttcagtg gaatcaatcc agagcgcatc gatgccgacc gagattaagc cattttttat
    12901 atatttccag taaagatcat tagcggctgg attgaaagcg tcgaaatatt tgaaatttgc
    12961 ccatccgacg gtattgtaga ggaacccgtt ttgctccatt tcttgataaa ttgcggtgtt
    13021 cggtccaagc cccggccaga ttgaaatcat gatatgaaag ttttgattgt ggatttgccg
    13081 gatcatttcc tcaggctcgg gatatttttt cggatcaaag aacatctggc tccaattttc
    13141 attgccgtcc cagtaattcc aatcctggat aatattatca attggtatgc gacgccgacg
    13201 atattcattc acaacgctta acaattcatc gcggttttta taatgctcct tgctttgcca
    13261 ataaccgtaa gcccattttc catacaaagg agcctttcca gttagataac gatacccact
    13321 aatgacggaa tccattgttc cgccataaat taagtaataa tcgagattgt caccgacttc
    13381 cgaccaaagt gacatcgtct cagccgtatc ctgaaaaata gtcttggagt aattatccca
    13441 cagaatgccc catccgcgag tagaaatcaa aaaaggagtt accgcgtccg tattggtttg
    13501 gacgagtttg acagttcgcc cgcggtaatt catatatccg tcttgatgtt gaccaagccc
    13561 gtagagccct tcgtctggtg ttaatttgaa attttgctga atgttgaaag ctttttcata
    13621 cggaagcgtt accggagtaa aaacggcttt tttattttct tttaaaatac ttgtgccgct
    13681 ggtcgtaaaa tattccaatt ggccatcatt cttaaccagg cgaattgtca ttcgctccga
    13741 ttttaggatt atgtcgttgt cattttcctc aaccgtaata gataaatccg gcaaactatc
    13801 tctaatgacc attaaactta gcttttcagc tgaagattcc ggccgccact taatgactcg
    13861 ggcgatgtcg tcagcgtaaa attggacacg tacattcaaa tcacgagttt ttaaatcaac
    13921 cccattctga atgggtttaa cttttccgga ctgaccgcat tgtgataccg ccagcgctaa
    13981 aatcattaac gaataagtaa tctgtgattt catttattcc cctcattttt taggtttgtt
    14041 aaaagaccgc tggctataat ttttttaata actatcggaa ctgatttaca tttgaccgga
    14101 cttgatggcg gctctgcttc aaaaaccttc gtaatatcaa tggaattata ttagtcaatc
    14161 ttcactatga aaacaagctt ttgaatggta aataagtagc agattaaacc gatggatcct
    14221 aaaattatat aattggacac tgaggcgcac agaggtaagc cggagagcac ggagcatttt
    14281 taagtacttt taagttctat taagtttttt taacattttt aagtgccttt aggaatttaa
    14341 tagtacttaa gcacacttat cagcactttc gagcatttga taaattaggc ttgtctcatt
    14401 gcgggagtaa tattaatttg attcggcgat gaacaatttt aaacgcaaga ttactcgtac
    14461 cgtatacgtc gggaatgttc ccgtcggcgg cggatttcca attccagtgc aatcaatgac
    14521 aaccgctaaa acgcacgact taaaggcgat tctgccgcaa attgagaaac tggaagccgc
    14581 cggttgtcag ataatccgcg tcaccgtccc cgatgaacaa gccctcaaag ctttaccgga
    14641 aataaaaaag cggatgacag tgccactggt cgccgatatt cattttgatt atcgcctggc
    14701 actgggcgca attgatgctg gtgccgataa aatccgaatt aaccccggca atattggtgg
    14761 cgcagagcga gtgaaagtcg ttctggcaaa agcccgagcg gcgcatattc ccattcgtat
    14821 cggcgtcaat tccggatcac tggaaaaaga cttgcgggca aaatacgggg gagcgacgcc
    14881 cgaagccatg ctggaaagcg cccgccgcca tctggaaatt tgcgaggaga atgatttccg
    14941 cgacgtaatc gttgccctca aatccagtga agccagtctg atgatcgccg ctaatcggct
    15001 tttttccgag aaatacgatt atccccttca tcttggtgtt accgaagccg gtccggtctg
    15061 gtcgggcaca attaaatccg ccattggcat cggaacgctt ttagctgaag gaatcggcga
    15121 cactttgcgg gtgtctctga cggcggatcc agtcgaagaa gtacgcgccg gttttgaaat
    15181 actaaaagcc ctaaatttaa ccaaaagtgg tatccgactg gtatcgtgtc ccacctgcgg
    15241 ccgtacctcg gttaatctga ttgaaatagt gaacgaagtc gagagtaaaa tccgcgatct
    15301 acctcaaaat ttaaccgttg ccgttatggg ctgtgaggtc aatggccccg gtgaagcccg
    15361 gtcggctgat ct
//
LOCUS       DAOSMP010000045        15151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_3948_length_15151_cov_19.817634, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000045 DAOSMP010000000
VERSION     DAOSMP010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 15151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15151
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3948_length_15151_cov_19.817634"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PKX09_07805"
     CDS             complement(<1..362)
                     /locus_tag="PKX09_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOU17763.1"
                     /translation="MKLSNLIKREAIVVPIQNTEKQLVIEELLEAAAATGQILDRAKA
                     LQALLDRESLGSTGLEKGLAIPHARTNAVDGLVISLGVAPDGIDFQAADGGLSYLFFL
                     LLASDALTSTYVQALALIA"
     gene            complement(398..901)
                     /locus_tag="PKX09_07810"
     CDS             complement(398..901)
                     /locus_tag="PKX09_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOU17764.1"
                     /translation="MPNTQSGRLISKILLLTDGSEGCISVAKYGIILSKITGAALKAI
                     YVVNTDLLKQLERARIFVKIEEVDYESDLEDDGQDYLQDITELAASKGVHLETELLKG
                     IVHKIAIQKIEEWNADLLIMGELEPGLSRQDISHDELGLLLRKARCSVLVAKEQRGLD
                     ELFETLT"
     gene            complement(894..1529)
                     /locus_tag="PKX09_07815"
     CDS             complement(894..1529)
                     /locus_tag="PKX09_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOU17765.1"
                     /translation="MANRIKLVPTKSNLLKLKRDLSFAQEGYELLEQKRQVLVSQLMA
                     MVNEAIVAHEKMDAALREAYSALQNAIISDGKSTVKSMARAVRIESDIQIYNLRVMGV
                     TIPKVEVEIEDDAPYYSAGNSSFWVDESIFRFKQILEVMGRFAELRVSLLRLAQEVRK
                     TQRRVNALEKIAIPDYQETIKFISDSLEESERGTLTSMKYIKEHLEKRKDA"
     gene            complement(1542..2984)
                     /locus_tag="PKX09_07820"
     CDS             complement(1542..2984)
                     /locus_tag="PKX09_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOU17766.1"
                     /translation="MSQIKEINEMNKYDQTELLKYREYIGIQEVAGPLICIENIHNVG
                     YNELVEIRDERGEPRIGQVLEANQSAAVIQVFEGTTGLSLAGTAVRFHGTPLRIPVTK
                     EMFGRIFDGLGRPIDGGPEPLTNEFWDANGESINPTARTYPTTFIQTGISAIDGMNTL
                     VNGQKLPLFSGPGLSHDRLTAQIVRQAKIVGREVSFAIVFAAMGIKHDIAQYFIDNFR
                     DAGVLENVVMFLSLADAPSVERLITPRSALTMAEYLAFHENMQVLVVMTDMTNYCEAL
                     REISIARGEIPSRKSYPGYLYSDLASLYERAGMVDNSTGSITQVPILSMPNDDISHPI
                     PDLTGYITEGQIVLDRELFQQGIYPPIALLPSLSRLMKDGIGEGLTRADHAHLASQLF
                     AAYARVKEVRALASVIGEEELSAVDHSYLKFGLAMENKFISQKETEDRSIYETLDLGW
                     KMLSLLPREELQRITEEEAQQYHHYQNENE"
     gene            complement(2997..4763)
                     /locus_tag="PKX09_07825"
     CDS             complement(2997..4763)
                     /locus_tag="PKX09_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOU17767.1"
                     /translation="MAIVIGKVTRVLGPVVHAIATAKVQMLEFVEVGTERLIGEVVRL
                     HGNQIIIQVYEDTTGLTPGVEVYGSGMPLSVELGPGLIGTIYDGVQRPLTEIKKVSDQ
                     YIHRGIKAPALDRNKKWSFEPAVSPGTIVSSGQVIGTVKETVSITQKILVPPNISGKV
                     ISIAPPGEYRIENEICVIENGQIRHTLSLFQRWPVRQPRPIKNRLSTSQPIITGQRVI
                     DTLFPIAKGGTVAIPGGFGTGKTMVEHQIAKWSDADVVVYIGCGERGNEMTDVLTEFP
                     QLIDPRTGRPLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYHVAVMADSTS
                     RWAEALRELSGRMEEMPAEEGFPAYLPTRLAEFYERAGYFETLNQETGSVTIVGAISP
                     PGGDFSEPVTQHTKRFVRNFLALDRDLANARHYPAISWLDSYSDYVEMVTAWWQEKAD
                     PDWAELRQEMLDLLQKEAKLQQVVKLVGADVLPESQRLILEVCTLFKNVFLQQSAYDQ
                     IDTYSTVQKQAKMLRIIVTFYRLSADVLRHGATLYKVKRTKTFRAIMNMKYEISNDEI
                     DKLDQILANMTHELASLSRPNF"
     gene            complement(4776..5399)
                     /locus_tag="PKX09_07830"
     CDS             complement(4776..5399)
                     /locus_tag="PKX09_07830"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HOU17768.1"
                     /translation="MPDKNATLEAIVAEIRQMSDAEIEAIRQSASREQAQIAHRARQE
                     AEKIRAGIISKAQQNAEKIRQRSAAQTNLEIKKLNLQSRLLITAEIRRQFEERLFALR
                     QSPEYAQLLKELIVEGIVGLGVDHILISAGDREHTILTDEFFRSIEASFNPPVHLELE
                     PVVLNEPGVILYSADKRRRFDNRLSRYVQRVLEKYHWEIMQKFSENN"
     assembly_gap    5463..5562
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5563..5873)
                     /locus_tag="PKX09_07835"
     CDS             complement(<5563..5873)
                     /locus_tag="PKX09_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HOU17769.1"
                     /translation="MLKIKSFKKLLAIVITLGFVAIGGTSLLVLKAIAQTPEKTATSA
                     VEGTETTVEVAPNQARDSDTIKFGFIAAALAVGIGSIGAAIAVGNIGTAAMGTIGEKP
                     E"
     gene            complement(5921..7885)
                     /locus_tag="PKX09_07840"
     CDS             complement(5921..7885)
                     /locus_tag="PKX09_07840"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOU17770.1"
                     /translation="MFFPEKMLLVNALFPDKYTPKVVRMIIHQGDLQIVDAAQTNPWI
                     KDLARTYSTEESSQSQARREKVEGLVKTLKLTDQLHNFQPLSGDWMEIDQSLETAIAE
                     IESVSQRRDSLQKEFQRLNEIKTRYDRFPGLGKALQMPGDYSYLAIETGEIPDNNLPL
                     LEEQLSATLHVILPIGRQKGMTSLVAIVLKRDAEVLQAALKASGFQPTKIEKELPSLA
                     TESIEKLEKELNDLNEQLRDAEEKFQAVVNRHRALLIGVYLRIRQEQVQGRMLHFFRR
                     TEKTCLFSGWLPDSRKEVFVKELQKATQNRAIVDLIPAESHEAVKDGSVEVPVKLNNP
                     AFFKPFEMLTSVYGLAKYQTIDPTPVFGLSFLLMFGMMFGDIGHGLVLALLGLALLLK
                     SHKDTFKSAGYLLLYAGGSSIIFGFLFGSFFGVEDLFPTIWMKPMNDINQLFKVVIYV
                     GAGMITVSIIINLINGIRSGRILDYIFDKAGLLSGILYWCGIILVSRLVLTKTNGGVG
                     LFVIYLFVACALLLFFREPILNLIRKNRRLYSSGVVTGLMHSLVEMLEIVLGFLANTV
                     SFIRVGAFGLAHAGLFIAIFALSDMVSGKLSGVASILILILGNIFIIALEGLVVTIQA
                     IRLEFYEFFNRFFRPARVKYQPIRENPNLE"
     gene            complement(7894..8925)
                     /locus_tag="PKX09_07845"
     CDS             complement(7894..8925)
                     /locus_tag="PKX09_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOU17771.1"
                     /translation="MSAVSTYGLVNARVRTRRSALLSASSYKALAAATDLPEMLNLLT
                     RYKNRLSFENLPDYSIEALERLLIQEKARQILDLKYSSRGPARDIMALMLSRIDAENV
                     KTILRAWQKSDQNVSNIIECTGLAVLPITKMLTAEDLTQFAAYINQLPFYEAFLKAAK
                     GFDRSKSLFEIEVAIDKSLYQSLWDLTALLNKTDQHIVRRMVGIEIDLKNLDWIARIR
                     KYYQSQIKVTETSLIPNGYRLPYATLRKMITTGSIEDGLEKILPGLTPITAQERDQLT
                     ALERLEQFLNQALFHEANRLFLENPLSIASTIGYYYLVHIETRNLCTLINAKYYKLAP
                     QAVLNKLVY"
     gene            complement(8925..9248)
                     /locus_tag="PKX09_07850"
     CDS             complement(8925..9248)
                     /locus_tag="PKX09_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17772.1"
                     /translation="MKEILQVIIDEEKSARERIENARLQARRDTEAAQIEAQKTIETA
                     RQLALQEAEALITTAKSEAERQHAELLNAARAQQNDIFNKYHAEIAAAVDFLFQTLPI
                     GKKVA"
     assembly_gap    9435..9534
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9535..10893
                     /locus_tag="PKX09_07855"
     CDS             <9535..10893
                     /locus_tag="PKX09_07855"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOU17773.1"
                     /translation="PVLLLFLGIGMLCGSDVLNLIYFDNAVLAQKVANIFLIFILFEG
                     GFHTRREIVQSVFGPALTLATFGIALTAGILGLLIHFIMGLDLLYSLLIGSIISSTDA
                     AAVFMIMRQRAIKKRVSLTLEVESATNDPMAIMLTIVFIQIITGHPQNIGLFILNLIW
                     QFGGGIVVGWLISKVGAFLFNHLKVENRGYYHVLSIAVCLLAYGSAEVVKANGIIAVF
                     FAGYWLGNTDFAYRKGVGNFIEGISTFSNIAIFLLLGVLVFPKSLLSVWQEGLLITAI
                     MILVARPLTILICTLPFNFKFREKVFLMWGGIKGAVPIVLATYPAVYGIDQNHFIFNI
                     VFFAVFLSCLLQGVTLTWGASKLRLCIPSRPKPIHSIELITTRKTDVDVFEIQLSDNS
                     KIDGLQIRDLALPPDSLIIAIIRDDQIIPPKGHTVLKKGDILFVIAAQDNVERISEIL
                     SK"
     gene            complement(10987..11121)
                     /locus_tag="PKX09_07860"
     CDS             complement(10987..11121)
                     /locus_tag="PKX09_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17774.1"
                     /translation="MDNYESAITALKIPFFPLRLRFPAGDVLDAVSNLNGQILGSPFY
                     "
     gene            11648..12127
                     /locus_tag="PKX09_07865"
     CDS             11648..12127
                     /locus_tag="PKX09_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOU17775.1"
                     /translation="MLFLKNLLQSRWGIALEDFYTELNKIKTAGTMVVIDACFSGSSN
                     EGALIKNISPVMIEIEKEHAVADHIVEFTSSSGDEVSSWFPEKKHSLYTYYFLKGLQG
                     EADQNGDKILTVGEMQYYLVEKVPYEARRLNNRQQTPGVTPDREKRILLHYTTSTQP"
     gene            12148..13014
                     /locus_tag="PKX09_07870"
     CDS             12148..13014
                     /locus_tag="PKX09_07870"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HOU17776.1"
                     /translation="MKQNKIIIGVIILSLMTVPAVCQSSKAIRVLIDTVLVFDSNTSP
                     AEAKNKTLAFARECAIKKAVPEEVMTSSVLTDLRAESGRQAQEQTAINIFAISTQAGY
                     IVEEKVISSEPKFANDILHYRLKLKALVEPTRGERDPSLSLELSVNRNVLKDGDPLFI
                     TAKSSTDGYLYLFNFLADNSVLLMFPNPISPDNAIKAGVPLRIPTAAEQKRGISYKVQ
                     ADSNKLVTAEAIFGVFCKNRIAEIDKFQTVRKDFVVLSAGEQSFTDFQKWLVRVPLSQ
                     RVEKVIPIQIFK"
     gene            complement(13105..13410)
                     /locus_tag="PKX09_07875"
     CDS             complement(13105..13410)
                     /locus_tag="PKX09_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOU17777.1"
                     /translation="MTDWIAIKKDEKHIAREKAKARELRKSNWWKQKIAAGICYYCGK
                     KFKPQELTMDHIVPIARGGKSIKSNVVPACKECNNKKKYLTPVEILMNRKDKSTPEN"
     gene            complement(13407..14099)
                     /locus_tag="PKX09_07880"
     CDS             complement(13407..14099)
                     /locus_tag="PKX09_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOU17778.1"
                     /translation="MTPDIKSHKKQSALSTSQTVIAFQKKVRKHFHGNYLLHLPPGYA
                     ESQNDWSTIFFLHGSGERGNDPEIVRRQGLPQVIDKKPDFPFIVISPQCPANRWWSLE
                     WLDALFDEVVKLYRIDQKRIYLTGLSMGGFATWDWAIEHPERFAAIAPICGGGNPLEA
                     DKIKDVPTWVFHGAQDEIVPLQKSQEMVAALKACGGKVKFTVYPNTGHDAWTKTYNNP
                     RLYEWFLKQTKK"
     gene            complement(14174..>15151)
                     /locus_tag="PKX09_07885"
     CDS             complement(14174..>15151)
                     /locus_tag="PKX09_07885"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOU17779.1"
                     /translation="PIDTQEAVRFIYRQAENLLAEVYPIAAITKGRQGAELTEMVELA
                     NAGAAAFSDDGTPLKNTQIMRYALEYSRLTGKPIINHAEDVDLKADGLMNEGVVSTHL
                     GLPGNPAIAEEIMINRDLQLAKFTSARLHVPHVSTAGSVELIRKAKAEGVRVTAEVTP
                     HHFSLTDEYMRTFDANGKVAPPLRTEADRQALIAGLKDGTIDAIATDHAPHTIDTKET
                     SLDLASYGMIGLETAFALAMTNLVHPGHLTLLQLIKLLTVNPAAIMNIALPKLEHGAD
                     ANLTIFDPNQWWVFNRKNIYSRSQNTPFIGAELTGRIKAVVTKSHYISL"
BASE COUNT         4002 a         3319 c         3387 g         4243 t
ORIGIN      
        1 gcgatcaatg caagagcctg aacataggtt gaggtcaatg catccgaagc cagcaataaa
       61 aagaaaaggt aagaaagacc gccgtcagcg gcttgaaaat ctatgccatc cggggcaacg
      121 cccagtgata tcaccagacc gtcaaccgca ttagtgcggg catgcgggat cgccagtcct
      181 ttctcgagtc cggtactgcc taaactctca cgatccaaaa gggcttgcaa agcttttgcg
      241 cggtcaagaa tctgacctgt ggccgcggcg gcttctagta gttcctcgat tactaattgt
      301 ttttcggtat tctgaattgg tacgacaatc gcctcgcgct taatcagatt ggaaagtttc
      361 attttaaaaa aagctcctcg ttttactttg acaaaattca ggttaaagtt tcaaataatt
      421 cgtcaagacc cctctgctct ttagctacga gcaccgaaca gcgcgcttta cgtaaaagca
      481 agccgagttc gtcgtgagag atgtcctgac gggacagacc tggctcaagt tcacccatta
      541 tcaggaggtc agcgttccat tcttcgatct tctggattgc gattttatgg acgatgcctt
      601 tcaataattc ggtttcaaga tgaacgccct tggaagcggc caattctgtg atgtcttgca
      661 gataatcttg accgtcatct tctagatcac tttcgtaatc gacttcttcg atttttacaa
      721 agatacgagc gcgctcgagc tgcttgagta aatcagtgtt gacgacataa attgctttga
      781 gagccgctcc agtaattttt gaaaggatga tgccatactt ggcaaccgaa atacatccct
      841 ctgaaccatc agtcagcagg agaatttttg aaatgagacg tcccgattgc gtattaggca
      901 tcctttcgtt tttcaaggtg ttctttgatg tatttcatcg aagtaagtgt tccacgctcg
      961 ctttcttcca aagaatctga aataaattta atagtttctt ggtaatcggg gatagcaatt
     1021 ttttccaggg cattgactcg ccgctgggtc ttgcggactt cctgagccag gcgtaaaagc
     1081 gaaacacgca actcagcaaa gcgtcccatc acctcgagaa tttgtttaaa tcggaaaata
     1141 ctttcgtcaa cccagaatga cgaattaccg gcactatagt aaggcgcatc atcctctatt
     1201 tcaacctcga ctttcgggat tgtcacaccc attacccgta gattgtaaat ctggatatcc
     1261 gattcaatcc gaactgcccg cgccatagac ttgaccgtgc tttttccatc ggaaataatg
     1321 gcattttgaa gagcactata cgcttcgcgc aaagccgcat ccattttttc atgcgccaca
     1381 atcgcttcat tgaccattgc catcaactgt gaaaccagca cctgacgctt ctgctctaaa
     1441 agttcatagc cttcctgggc aaagctcaaa tcgcgcttta atttgagcag attacttttg
     1501 gtcgggacga gtttgatgcg gtttgccatt ctactaatta tttattcatt ttcattttgg
     1561 taatggtgat actgctgggc ttcttcttca gtaatgcgtt gtaattcttc ccgcggcagg
     1621 agcgacaaca tcttccagcc taaatcgagg gtttcataaa tagaacgatc ttcggtttct
     1681 ttctgagaaa taaatttgtt ttccatagcc agaccgaatt ttaagtaact gtggtcaact
     1741 gcactgagtt cttcctcacc aatgacggaa gccagagcac gtacctcctt cacccgggca
     1801 taagccgcaa atagctggct ggctaaatga gcatgatcgg cgcgggtcaa accttccccg
     1861 attccatctt tcatcagacg cgaaagagaa ggtaagaggg cgatcggcgg ataaattccc
     1921 tgctggaaaa gttcgcgatc cagaacgatt tgtccttcgg taatatatcc cgtcaaatcg
     1981 ggaataggat gcgaaatatc atcattcggc atcgagagga tgggaacttg agtaatcgaa
     2041 ccagttgagt tatctaccat accggcgcgt tcgtaaagcg aagctaggtc ggagtataga
     2101 tagccaggat aacttttgcg ggacgggatt tcaccgcggg caatcgaaat ttctcgcaat
     2161 gcctcacaat agttggtcat atcggtcata accaccagca cttgcatatt ttcatgaaag
     2221 gctaaatatt cagccatagt caaagccgaa cgcggcgtga tcaggcgttc aaccgacgga
     2281 gcatcagcca atgacaggaa cattacgaca ttctccagaa cgccggcatc ccggaaatta
     2341 tctatgaagt attgagcaat gtcgtgcttg atgcccatcg cggcaaagac aatggcaaag
     2401 gatacctcgc ggcctacaat tttagcttga cgaacaattt gagccgtcaa acgatcgtgt
     2461 gataaacctg ggccagaaaa tagcggcagt ttctgtccat tgaccagagt gttcatcccg
     2521 tcaatagctg aaatccccgt ctgaataaaa gtagttggat atgtccgggc ggttggattt
     2581 atagattcac cgttggcgtc ccagaattca ttggtcaagg gctccggtcc accgtcgatt
     2641 gggcgtccta aaccatcgaa gattcgcccg aacatttctt tggtaacggg aattcgcaga
     2701 ggcgtgccat gaaaccgcac tgctgttccg gctaatgata aaccggtggt gccttcgaaa
     2761 acctgaataa cggcggcgga ttggttggct tccaggacct ggccaatgcg tggttcgccg
     2821 cgttcatcgc ggatttcgac taattcatta tagccaacat tatggatatt ttcgatacaa
     2881 attaatggcc cagcaacttc ttgaattcca atatattccc ggtatttcaa gagttcagtc
     2941 tggtcatatt tattcatttc attaatttct ttgatctggg acattcgatt ataaccttaa
     3001 aaatttgggc ggctgaggct ggctaattcg tgggtcatat tagccaagat ttgatctaat
     3061 ttatcaatct cgtcattgga aatttcatat ttcatattca taatggcacg aaaggtttta
     3121 gtgcgtttaa ccttataaag agtagcgcca tgtctgagga catcggccga taaccgataa
     3181 aacgtaacga tgatgcgaag cattttcgcc tgtttttgaa cagtggaata ggtatctatc
     3241 tggtcgtaag ccgattgttg gagaaaaacg tttttgaaaa gggtacagac ttccagaatg
     3301 agacgttgag actccggtag gacatccgca ccgactaatt tgacgacctg ttgaagttta
     3361 gcttctttct ggagaagatc gagcatttcc tgtcgcaact cagcccaatc cggatcggct
     3421 ttctcctgcc accaggcagt caccatttcg acataatcgg aataagaatc gagccaggaa
     3481 atcgccggat aatggcgcgc attagccaga tcgcgatcaa gcgccaggaa attgcggacg
     3541 aaacgcttgg tatgttgagt caccggctcc gagaaatcac caccgggcgg tgaaatagcg
     3601 cccacgatgg taaccgagcc ggtttcctgg ttgagagttt cgaaataacc ggcgcgctcg
     3661 taaaattcag ctaagcgagt tggcaaataa gctgggaaac cttcttcggc tggcatttct
     3721 tccatacggc cagacaattc gcgcaaagct tccgcccagc gcgaagttga gtcagccata
     3781 acggcgacgt gataacccat atcccggaaa tattcagcaa tggtgatgcc ggtgtaaatc
     3841 gaagcctcac gtgcggcaac cggcatattg gaagtattgg caatcagtac ggtgcgttcc
     3901 atcagcggtc gcccggtgcg cggatcaatt aattgaggaa attcagtcaa gacatcagtc
     3961 atttcgttgc cgcgttcacc acagccaata tagacgacga catcggcatc tgaccacttg
     4021 gcgatctggt gttcaaccat agttttaccg gtaccaaatc cacccggaat agcgactgtg
     4081 ccacctttgg caatcgggaa aagggtatca ataacgcgct gtccggttat aatcggttga
     4141 ctggtggaca aacggttttt aattggtcgt ggctgacgca ctggccagcg ctgaaaaagg
     4201 gacaatgtat gtcggatttg accattttcg ataacacaaa tttcattttc gatacgatat
     4261 tctcccggcg gcgcaatgct aatgaccttg ccggaaatgt ttggcggaac taatattttt
     4321 tgagtaatgg aaaccgtttc ctttaccgtc ccgattacct gaccactgct aacgattgta
     4381 ccgggactta cagctggttc gaatgaccat tttttattgc ggtcgagagc aggagctttg
     4441 atgccacgat gaatatattg atcggaaacc ttcttgattt ccgtcagcgg gcgttgaaca
     4501 ccgtcatata tagtgccaat cagaccgggc ccgagttcca ctgaaagggg cattccggaa
     4561 ccgtacactt ctactccggg cgttaaaccg gtagtatctt cataaacttg aatgatgatt
     4621 tgattgccgt gaagccgcac aacttcgccg atcaggcgtt cggtcccaac ctcgacaaat
     4681 tcgagcatct gaaccttagc agttgcgatt gcatgaacaa ctggtcccaa aacgcgggtg
     4741 acttttccaa taactattgc caccgtttac ctcttttaat tattttccga gaatttctgc
     4801 ataatttccc agtgatattt ttccaggaca cgctgaacat aacgggataa gcggttatcg
     4861 aatcgcctcc gcttatctgc cgaataaaga atgacccccg gctcattgag aaccacaggc
     4921 tctagttcaa ggtgcaccgg tggattaaaa gatgcctcaa ttgatctaaa aaattcatcg
     4981 gttaggatgg tgtgctctcg atcgccagcg ctaatgagaa tgtgatcaac tcccaagccc
     5041 acaattcctt ccacaattag ctctttcagc aattgggcat attcaggaga ttggcgcaaa
     5101 gcaaataaac gttcttcaaa ttgacgccgg atttcggcag tgattaaaag tcgtgattgc
     5161 aaattcaatt ttttgatttc aagattggtt tgagccgctg atcgctggcg aattttctcg
     5221 gcattttgct gggctttact gataattccg gcgcgaattt tttccgcctc ttggcgggca
     5281 cggtgagcaa tttgggcttg ctcgcggctg gcagactgac ggatggcttc aatttcagca
     5341 tccgacatct gacgaatttc agctacaatg gcttctagag ttgcgttttt atcaggcata
     5401 attcctcatt ctaaccgaac accaaccgca gtttggacaa catctcgcac tgaaatgcgg
     5461 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaattctgg tttttcgcca
     5581 attgtgccca tagccgcggt accaatattg ccaacggcaa tggcggctcc gatcgaaccg
     5641 atgccaactg ccagagcggc ggcaataaaa ccaaatttga ttgtatcgga atcgcgagcc
     5701 tgattcggag cgacttcgac tgtggtttca gtaccctcta ctgcactcgt tgcggttttt
     5761 tccggagtct gcgcaatagc cttcagcacc agtagtgaag ttccgccaat tgccacgaaa
     5821 cccagcgtaa tcacaattgc cagcagtttt ttaaaggatt tgatttttaa catatttgca
     5881 ttacctccgc aaatttattt ttcatattat tagagaatat ttactccagg ttgggattct
     5941 ctctgattgg ttgatactta accctcgctg gacggaaaaa gcgattgaaa aattcataaa
     6001 attccagacg aattgcctgg atggttacta ctagtccttc cagagcaatg atgaaaatat
     6061 tgcctaaaat tagaattaag atagaagcta ctccacttaa tttgccactc accatatccg
     6121 aaagagcgaa tatggcgata aataatccgg catgggctaa tccaaacgct cccacacgga
     6181 tgaaagaaac cgtattagcc aaaaatccca gcacaatctc aagcatttct acaagcgaat
     6241 gcattaatcc ggtgacaact cccgaactat atagacgccg gtttttgcgg attaaattaa
     6301 ggattggttc ccggaagaaa agaagcagag cgcaagccac aaagagatag atcacaaaaa
     6361 ggccaactcc accattagtt ttcgtgagta ccagacgact taccaatatg ataccgcacc
     6421 aatagagaat gccgctcagt aaaccggctt tgtcaaaaat ataatcgaga attctgccgc
     6481 tacggatgcc atttattaaa tttataataa tcgagacggt tatcatcccg gcaccaacgt
     6541 agatcacaac tttgaacaat tgattgatgt cattcatcgg tttcatccag atggttggaa
     6601 ataaatcttc gaccccgaaa aaactaccga atagaaaacc gaaaataata ctggaaccac
     6661 cggcgtagag tagtaaatag ccggccgatt tgaatgtatc tttgtgactt tttaatagca
     6721 acgctaagcc aagcaatgcc aagaccagac cgtgcccaat atctccaaac atcattccga
     6781 acatcaaaag aaaacttaga ccaaacaccg gtgttgggtc aatggtctga tacttcgcca
     6841 gaccatagac cgaagtcagc atctcaaagg gtttgaaaaa ggcggggtta tttaatttga
     6901 ccgggacttc cacactacca tctttgactg cttcatgcga ttcagccggt atcaggtcaa
     6961 cgatagcccg attttgggtg gctttctgca attctttgac aaaaacctcc ttgcggctat
     7021 ccggcaacca gcccgaaaaa agacaggtct tttcagtacg gcggaagaaa tgtaacatcc
     7081 ggccttgaac ttgttcctga cgaatacgta ggtaaacgcc gattaggagc gcgcgatggc
     7141 gattgactac cgcctggaat ttttcctcgg cgtcacgcag ttgttcattg agatcgttca
     7201 gttctttttc gagtttttca attgattcgg tggcgagact gggcaattct ttttcaattt
     7261 tggtcggctg gaaaccgctg gctttgagcg ccgcctgaag aacttcggca tcgcgtttga
     7321 gaacgattgc caccagtgag gtcattcctt tctgtctccc gatcggtaga attacatgca
     7381 gagtagcgga taactgctct tccagtagcg gtaggttatt gtcggggatt tcgcctgttt
     7441 caatagcaag ataggaatag tcacccggca tttgaagggc tttgccgaga ccgggaaagc
     7501 gatcgtagcg ggttttgatt tcattgagac gctgaaattc cttttgcagg ctgtcgcgcc
     7561 tctgactaac tgattcgatt tcagcaatgg cagtctcgag gctctgatca atttccatcc
     7621 aatcaccgct tagcggttga aagttatgga gttggtcggt taatttaagg gttttaacta
     7681 acccttccac tttttcccga cgggcttgag attgagagga ttcttccgtt gaatatgttc
     7741 gtgcgaggtc tttgatccaa ggattagttt gggcggcatc cacaatttgc aaatcgccct
     7801 gatgtataat catgcggaca accttgggcg tatatttgtc gggaaagagc gcattgacca
     7861 aaagcatttt ttctgggaaa aacatcttta tcctcaatat accaatttat ttaaaaccgc
     7921 ctgcggtgct aatttataat atttagcatt tattaatgtg cagagattcc gggtttcgat
     7981 atgaactaga tagtagtagc caattgtgga ggcgatagaa agtggatttt ccagaaaaag
     8041 gcggttagct tcatgaaaaa gcgcctgatt gagaaattgc tctaagcgtt caagagcagt
     8101 aagttgatca cgttcctgcg cagtaatggg cgtcaatccc ggtaatattt tttccagtcc
     8161 gtcttcgatt gagccggtag taatcatttt tcggagagta gcataaggca aacgatagcc
     8221 gttgggtatt aaacttgtct cggtgacttt aatttgagac tggtaatatt tacggatgcg
     8281 ggcgatccaa tccaggtttt tcaggtcaat ttcgatgccg accattcgtc gtactatatg
     8341 ttgatcggtt ttattcaata aagcagtcag gtcccaaagg gactgataca ggcttttatc
     8401 aatggcaact tcaatttcaa agaggctctt gcttcggtca aaacctttag cggctttcag
     8461 gaaagcttca taaaacggta gctgattgat ataagcggca aattgggtta aatcctccgc
     8521 agtcagcatt ttagttatcg gcagaaccgc caagccagtg cattcgataa tgttactgac
     8581 attctggtcg gatttttgcc aagccctgag aatagttttg acgttttcag catcaattct
     8641 tgagagcatc aaagccataa tgtcccgagc tggtccgcga ctggagtact tcaaatcaag
     8701 tatctgacgg gctttctctt ggattaataa ccgctccaaa gcttcaatcg aataatcagg
     8761 aaggttttcg aaactgagac gatttttata gcgggttaag agattaagca tttccggtaa
     8821 atcagttgca gcggctaaag ccttataaga tgaagcagaa agcaaggcgg agcgtcgtgt
     8881 ccggacgcga gcatttacca gtccgtaagt gctaacagca ctcattaggc gacctttttg
     8941 ccaattggaa gggtttgaaa aaggaaatcc acagcggcgg cgatttcagc atggtattta
     9001 ttgaaaatgt cgttttgttg cgctcgagcg gcatttaaca gctcggcgtg ttggcgttca
     9061 gcttcggatt tggcggtcgt aataagcgct tcggcttcct gtaatgccaa ttggcgagcg
     9121 gtttcaatgg ttttttgggc ttcaatctga gcggcttctg tatcgcggcg ggcttgaaga
     9181 cgagcgtttt ctattcgttc gcgggcagat ttttcttcgt ctattattac ctgaaggatt
     9241 tctttcaagt taacctcaag tgtctgccgg tttagatatc caggctatca ttggtaagtt
     9301 tgtaaaccgg cttaaaataa atactattca agctgacatt ttcaaataat ttcacagaat
     9361 ataatttagt caaaattaag tttattacta aactaaatct tgtaggtaag taggtgaata
     9421 aatcactttt aaatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccggtg
     9541 cttctgctat ttctggggat cgggatgcta tgcggtagtg atgtgctcaa tctgatttat
     9601 tttgacaatg ctgtactggc tcaaaaagta gccaatatct ttttaatatt cattttattc
     9661 gaaggtggtt ttcatacccg tcgcgaaata gtgcaatctg ttttcggccc ggctttgaca
     9721 ctcgcgacgt tcggtattgc tctgacggcg ggaattttag gacttctgat acattttatt
     9781 atggggctcg atttacttta ttcgttgttg attggctcca tcatttcttc aaccgatgcc
     9841 gcggcagtat ttatgattat gcgtcagcgt gccattaaaa agcgggtttc cttgacactg
     9901 gaagtcgaat cggctaccaa tgacccgatg gctataatgt tgacaattgt ttttattcaa
     9961 ataattacgg ggcatcccca gaacatcgga ctttttatcc tcaatttaat ctggcaattt
    10021 ggtggtggaa tagtggtagg ctggcttatt agtaaagtcg gcgcgtttct gttcaatcac
    10081 ctcaaggtgg aaaatcgtgg ctattatcat gtgctaagca tagcggtctg cttgctggcg
    10141 tatggaagtg cagaggttgt caaagctaat ggcattatcg cggttttttt tgccggatat
    10201 tggttaggaa atacggattt tgcttatcgc aaaggggtcg gcaattttat tgaagggatt
    10261 tcgactttct ctaatatcgc gatcttttta ctgctgggag ttctggtttt tcccaaaagc
    10321 ttattgtcgg tttggcaaga aggattatta attactgcga taatgatctt agttgctcgt
    10381 ccactgacta ttttgatttg cactctgcca tttaatttta aatttcgaga aaaagtgttc
    10441 ctgatgtggg gcggcatcaa gggggctgtt cccattgttt tggcaactta tccagctgtt
    10501 tatggcattg accagaatca ttttatcttc aatattgttt tctttgccgt ttttttgtcg
    10561 tgtttattac aaggggtcac acttacctgg ggcgcttcga aattacgact ctgtattcca
    10621 tctcgaccga aaccaatcca ttcgattgaa ttgatcacga cccgaaaaac cgatgtggat
    10681 gtctttgaaa ttcagttatc cgataattcc aaaattgacg gtttacaaat ccgcgactta
    10741 gctttaccgc ccgattcctt aattattgcc attattcgag atgatcaaat cattccgcct
    10801 aaaggtcata ctgtattgaa aaaaggtgat attttattcg taattgccgc tcaggacaat
    10861 gtagaacgga taagcgaaat tctaagtaaa taacctaaaa taagtaaaag aggtcgaggc
    10921 agagaagggt cattagtcat tggtcattag tcattagtaa agaggttgag taaatagtaa
    10981 agaggcttag taaaaaggag aacccaaaat ctgaccgttc aaattactta cggcatcaag
    11041 aacatctccg gctgggaagc gaagtctcag cggaaaaaat ggaattttca gcgctgtaat
    11101 agcactttcg taattatcca ctaccttttc ctatgtcact gatgttatat gaatttgata
    11161 acttttgcgc cttaaattac attcgctggg acagagcgaa cccccgtgag atgttcttta
    11221 gccgttgcct tttttggttc ttttttcggc aaaggaaaaa agaacaggaa agttagtcat
    11281 tggtcattag tcattagtaa agaggctgag aaaagagaag acctacagat atttggtcac
    11341 aggtaatagg ttttagatta aaaatacttc actaattctc cagtggcatc gcagaagtct
    11401 ccgatgagaa attcctgcct ggcagggcat tattggatta aattaataaa atcgtaatgc
    11461 gggatcgggc aaacgattta ttatgtggcg ataaacttat aattcgtaat atcctctgtt
    11521 aattacatag tagattaaag aaatagtcac caattttttc acgactcgaa tttttcaaaa
    11581 aaagattgct attttagcag aaaactgcta taattaacta atggaagttt ttatagcgcg
    11641 ataattattg ctatttctaa aaaatttatt acaaagtagg tggggcattg cacttgaaga
    11701 cttttacacg gaactaaata aaattaaaac tgccggcaca atggtggtca ttgacgcctg
    11761 tttctccggc tccagcaatg aaggagcgtt gattaaaaat atcagcccgg taatgattga
    11821 aatcgagaag gaacacgccg ttgccgatca tattgtcgaa tttacgtctt catcgggcga
    11881 tgaagttagt tcctggtttc ctgaaaaaaa acatagcctc tacacctatt atttcttaaa
    11941 aggtttgcaa ggcgaggctg atcagaacgg agacaaaatc ctaactgttg gagaaatgca
    12001 atactatctt gtcgagaaag tcccctatga agcccgccgc ctgaataatc gccaacagac
    12061 ccccggcgta actccagatc gagaaaagcg gatacttcta cattatacta cttcaactca
    12121 gccgtaattt ataattcggg taaagcaatg aagcaaaata aaattattat cggcgtaatt
    12181 atacttagcc tgatgactgt tcccgccgtc tgtcaatcgt cgaaagccat ccgcgtttta
    12241 attgatacgg ttctggtttt tgatagtaat acatccccgg ctgaagccaa aaacaaaacc
    12301 ctggcctttg cccgcgagtg cgccatcaaa aaagccgttc ctgaggaagt aatgacctcc
    12361 tccgtcctga ccgatttgcg agccgaatcc ggtcgccagg cgcaggagca gacagcaatc
    12421 aacattttcg caatttcaac tcaagcgggc tacattgtag aagagaaagt catctcatct
    12481 gagccgaaat tcgccaatga tattttgcat tatcgcctaa agcttaaggc tctggtcgag
    12541 ccgactcgcg gcgaacgcga tccgtcattg tcgctcgaat tatccgtcaa tcgcaatgtc
    12601 ctgaaagatg gcgatccgct gttcatcacg gctaaatctt ctaccgacgg ctatctctac
    12661 cttttcaatt ttttagcgga caattcggtt ctactaatgt tcccgaatcc aatatcacca
    12721 gataatgcca ttaaagccgg tgtccctctg cggattccga ctgccgccga acagaaaaga
    12781 ggcatttcct ataaagtcca ggctgattcc aataaattgg taacggctga agccattttc
    12841 ggagtgtttt gtaaaaaccg aatcgctgag attgataagt ttcaaaccgt gcgcaaggat
    12901 ttcgtagtgc tgtctgccgg agagcagagt tttaccgatt tccagaaatg gctggtgcgc
    12961 gtgccgctct cccaacgcgt cgagaaagtc atccccatcc agattttcaa ataaaagtaa
    13021 aacttgggtt aagccaagct agaaaagctt tgtctcaatc gaaaatccaa tgccttggct
    13081 taacataagt tgaatatcgc ttaattaatt ttcgggagtt gatttatctt tccggttcat
    13141 caatatttcc actggcgtta ggtatttctt cttattattg cattctttgc aggcagggac
    13201 gacgttgctt ttgatgcttt ttccgccgcg ggcaatcggt acgatatgat ccatcgttaa
    13261 ttcctgcggc ttaaatttct tgccgcagta atagcatatc ccggccgcta ttttctgttt
    13321 ccaccagttg gatttgcgta attctctggc tttcgctttt tcgcgagcaa tatgcttctc
    13381 gtcttttttt attgcgatcc aatcagtcat ttttttgtct gttttagaaa ccattcgtag
    13441 agtctgggat tattataagt cttcgtccag gcgtcgtgac cggtgttcgg ataaacagta
    13501 aatttcactt ttccgccgca agctttcagc gccgcaacca tctcctgtga tttttgaagc
    13561 ggcacgattt catcttgggc accatgaaaa acccaggtcg gaacatcttt tattttgtcg
    13621 gcttcgagcg gattgccacc gccgcaaatc ggcgctatcg ccgcaaaacg ttccgggtgt
    13681 tcgatagccc agtcccaggt agcaaagccg cccatactca atccggttaa ataaatacgt
    13741 ttctgatcaa ttcgatagag cttgaccact tcatcgaata gtgcgtccag ccattccagc
    13801 gaccaccagc gattggcggg acattgaggc gagattacga tgaacggaaa atctggtttt
    13861 ttatcaataa cttgggggag tccctggcgc cggacgattt cgggatcgtt ccctcgttca
    13921 ccggagccgt gtaggaagaa gatcgttgac cagtcatttt gagattcggc ataaccgggc
    13981 ggcaaatgga gcaagtaatt tccgtggaaa tgcttccgga ctttcttttg aaaggctatc
    14041 actgtttggg aggttgatag cgctgattgt tttttatgcg atttgatgtc tggcgtcatt
    14101 cgacaattaa tttaaataag attaagcgta tcctcaactt tcaggaattt cgcttcaggt
    14161 ttggggagag tgcttataat gaaatgtaat gcgatttggt gacgaccgct ttgatccgtc
    14221 cggtcaattc cgccccgata aaaggtgtat tctgagagcg cgaatatata tttttacgat
    14281 tgaaaaccca ccactggttg ggatcgaaaa tcgtcagatt agcgtccgcg ccgtgttcta
    14341 atttcggcag ggcgatgttc attatcgcgg cgggattgac cgtcagcaat tttatcaatt
    14401 gtaaaagagt cagatgccct ggatgtacca aattggtcat agccagcgca aaagcggttt
    14461 ccaatccgat catcccatac gatgcgaggt ctaacgaagt ttctttggtg tcaatcgtat
    14521 ggggcgcatg gtcggtagcg atagcgtcaa tcgtgccgtc cttaagccca gcaatcaaag
    14581 cctggcgatc ggcttcggtt cgcagaggcg gagcgacttt gccattggca tcaaaagtgc
    14641 gcatatattc gtctgtcaag gaaaaatgat gcggcgtaac ttcagcggtt acgcgcacgc
    14701 cttcagcttt agctttgcgg atcaattcca cggaaccggc ggtcgagaca tgtgggacgt
    14761 gtaaacgcgc tgaggtaaat ttcgccaatt gcaaatcgcg attaatcatt atttcctcag
    14821 cgatagcagg attacccggt aatcccagat gggtcgagac aacgccttca ttcatcaagc
    14881 cgtcggcttt taaatcaaca tcttcggcat ggttgataat tggtttgccg gtcaagcggg
    14941 aatattcgag agcatagcgc ataatctggg tgtttttcaa gggagtgccg tcatcggaga
    15001 aagccgccgc accagcatta gctaattcaa ccatttcggt caactcggcg ccctgacgac
    15061 ctttggtgat tgccgctatc ggataaactt ccgccaaaag gttttcggct tgccggtaga
    15121 taaaacgcac ggcttcttga gtatcaatgg g
//
LOCUS       DAOSMP010000046        14515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_4184_length_14515_cov_15.676763, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000046 DAOSMP010000000
VERSION     DAOSMP010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 14515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14515
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4184_length_14515_cov_15.676763"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="PKX09_07890"
     CDS             complement(<1..779)
                     /locus_tag="PKX09_07890"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOU17780.1"
                     /translation="MAKKIFSASLIFLVFSGLVFGATIKVPDDQSSIQAGIDVAAAGD
                     VILVAEGTYKEKIDFKGKKITVASLFYNDGKTDHIQKTIIDGQATGTVVKFSLGEDSS
                     SVLIGFTVTNGSADYGGGIYCYNSSPTIKNVVIRDNVAKYSGAGIHCYSSSPTLERVV
                     IYNNKANESGGTGGGICCFNNSNPVLRNVTIAKNTAKSNGNDIACQYASKITIVNSII
                     WSEYLPDPIYIEGTGSISATFSDIKNGTGKSYFGEGCIATDP"
     gene            complement(990..3065)
                     /locus_tag="PKX09_07895"
     CDS             complement(990..3065)
                     /locus_tag="PKX09_07895"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17781.1"
                     /translation="MKNKLKITGLFLFLAISLVVIFDCGKITSPGGIEIPENPRIKTI
                     DDNNIEVSWDYDQSDQDVFFTVARKVGDANWNLEYAQTYDNFLIDEIPTNDSLIYAYK
                     IKTESDTSELASEFSDVVAYFSPKTIPTELAISVLESDKVEVTWWDNCIGEDGYKIEK
                     KVSGGNWIKDYKTLGRNGTSFTDKTSFGDSVFYRVYAFCGISVSGSAEGFGVATFPPP
                     YNLEYERIAIDKIKLTWQSKTSNVAGFKIDKKVGSADWQVEIATLDSNQTGWIDENAE
                     INETLQYRVYGFSEEYISSYVTTDEIDNTFPAPSNLTLTKIDEDVVKISWQDNSQGES
                     GFYIDRKVGMSDWVNGYASVDSNQTTFLDNISESCGTFYYRVKAFKGEHFSEPTDEGN
                     INIRLNLVSSLVTKGTATEVFVSDWKAYIADHYNGLIVIDLINPSSPSTDNNLVLPLP
                     DRTLSIYIYNNLAYATNHTGGLNIVDVTTLELIGNCQTSGVPVDVFVTGEPPYAYLCE
                     EEKGMSIASILSSTPHIISNITPPNNSADEKAKKLFVANGYAYVAYGSRGLTIFDVSD
                     VKNPIYVADFPSSSITQDVFIKDNYAFLADGENGLVMVDVSSVEAPSLVSNCSTHGFA
                     YSIYVRGNYAYLADKEKGLIVVNISDLANPFILGSFEMSTTPNSVFVDGSYAFVTDSE
                     GLKIIQIAP"
     gene            complement(3128..4315)
                     /locus_tag="PKX09_07900"
     CDS             complement(3128..4315)
                     /locus_tag="PKX09_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17782.1"
                     /translation="MKKKALFICLLLFFSSTNSQVNLIKSPSNDELITISPEVLVADA
                     LKMIEALAQKYEGRKIVNMSTYTGPISIPITQIYWKNALNLIANYNRLVVEELPGAYV
                     VKDFMAEEKAVEEKKPADEVVPDTKQVRISSIFFTADRSIINSLGIDWSTIYNGTVVA
                     SLDFTGGAEVLSDIISGTVSTTLESGEAVIELNALFKMIEHYNKGTVIARPSVTVISG
                     KQGYLQIGQDFSVRTTDFSGNLVDRFYSAGLILEVTPTIIQDSSKEAIHLVARVEKSS
                     VVPSDVQVIVNKNQATTEAILYDGEETVVAGLYDTEDIVVRKGVPILKDLPWWVLGLR
                     YIFGYSNHEKNEREIIIILKAELIEPIEVRMAKKESARELIEKYYKPKEHIDTLLFKK
                     VQE"
     gene            complement(4319..5185)
                     /locus_tag="PKX09_07905"
     CDS             complement(4319..5185)
                     /locus_tag="PKX09_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17783.1"
                     /translation="MPYKIRNTIALAVILLIVLIGGVISNSSSERKIADLSKKNQELS
                     KRLNMLNSQLENIGEEEELEQEIRELEIEISKFKKAIVKEDNPTITYDYLTQICQKFC
                     PEMVFDFKPAESGEIQGTKYNDYLIAGIVPFNSLYTFIYQIENQPKLYLLELLEIREQ
                     PASEDIIPFAFKLRAFYDENGIEPESIPIREIKTVNIRFNPFYPGIHDPVQIKEEEQY
                     LNPEEAILVGLTPTIAFFKDKNGRTISLTTGEKVAYGYLSKIDWENQAVTFKINRIGV
                     YVEKTIYLQGKG"
     gene            complement(5187..7346)
                     /locus_tag="PKX09_07910"
     CDS             complement(5187..7346)
                     /locus_tag="PKX09_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU17784.1"
                     /translation="MKNYYGIFQDGLDIQIAHLVRDEDLIKIQNLGETTLSEPLYTKK
                     EVNKIPTIELSDTNLEIQNNPDLKEEGFDLPEISEFDEFESNKLTEEITEVNAEGGTS
                     TSELQRILSNFSLETGKIALNANEEQISYHIFNSSLTFSAIKKKIKTEILSQEELKSR
                     KYAIGCIPNYDGMTLAFLHRGEFDLLNAILQVRPLLSPKRYIFSYIDTNEIALMNLIR
                     ANYNFPENDYVLILYVGSEYKAGIVMRNNTHVKTFKIIGDTSSRSENIRQVIYSKIIL
                     EQDVSDIPPVQHLILTGNMVRDEDIRFFSSKFDETTEVSRFEFKNINIATINRKYSPE
                     NIAKYAIPISLALKAADPKNKAFYNTNLLPPKIIESQKRFKIGWHGFILFIAIFYFTY
                     SATLKNLENNRQIVELQRQNYNIESELRHKNLMFARANELSQQITGKRKSMDKIKTIS
                     GNKSQWHRTIIALSDAFAKNPISWINYLRSDTSGFMVQGTTNHKENIAYITRYFPGCI
                     VSDVKTSEIQSIPVMRFVMNFSYSDQEEWVMKVAKPAPVTPQSGKTAAPPPITPAPKS
                     IESESAPKLAAPAPAVVQSQSPPVQSTPRITPYTVYREAAETYLSGNIQEGYHKLTQF
                     VEKYPNSPFTYSANYLIGECLFLMGEVAQAKDIFEKIANQDGTKTPDALLMLGRCYNA
                     LGDKAKAIDCWGMLITKYPEHKLAKMAEEKIKYVTEH"
     gene            complement(7376..8599)
                     /locus_tag="PKX09_07915"
     CDS             complement(7376..8599)
                     /locus_tag="PKX09_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOU17785.1"
                     /translation="MGLSFTPEMEIALPEYLIGTARFKNINKLITFNNAWKKEAYDNL
                     KSLLKKMEEIDASDIDIGGSMLDVKIWYRVYGRKYPDLEAKRYSNDEITAILLSILSD
                     DQKIILFDKKNIDFSLGISFGEKEANRFRGDIYYDNDVLVGNFRRVNRELFPIENLNI
                     PEPVLKRLNLRHEKSGLILITGITGSGKSTTLDSIINLNNEENEGHIVIISDPLEFIH
                     KSKKCIIRHREVGGDVLSFREGTIQALRQDPDIIIVGEMRDAPTIATVLEATDSGHKV
                     FTTLHTSSAVESIHRIIAEFLPEEQERIRLRLADTLSVVISQKLVPDRDNKLTLVKEI
                     LSVTDSVKAAIRNKNIGEIYQMITEGKKYGMVTMEQDLHNLYTKKIISRETALDFSNN
                     RKRIEQLLAYREICV"
     gene            complement(8695..9552)
                     /locus_tag="PKX09_07920"
     CDS             complement(8695..9552)
                     /locus_tag="PKX09_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU17786.1"
                     /translation="MQSHILVVDDDQTIRMLCKEILEEAGYKVTLAADGEEALEKMDY
                     DDFDLYLVDMVMPKMDGLELMKKIKQKQPLAVVMILTGFSSIEGAIKAVHAGAFQYLS
                     KPINIDELLNAVKEGIKYSQDLYGPLLKIFEPGTELVEKGEMAILSGFSEEDKQDFMI
                     LSSIYNFEPGENIRIADKMASSIILIESGEVSVWLSNTSVDYLKKWDTWNEESFVINS
                     PTSSVLRAETAVTARFFERKKLLEFFAFKGEKLLKKFMINLINCLYIKWRKSIQRIIM
                     LKLVTTESE"
     gene            complement(9559..9837)
                     /locus_tag="PKX09_07925"
     CDS             complement(9559..9837)
                     /locus_tag="PKX09_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17787.1"
                     /translation="MSDQEELIRKYEEVVEVAASIASIDHDINNQLMVFALVERRLQN
                     AAQQYNDEKLLRSSQQVSEALDNIKRILSRLQNLKKLDLIKEKREKRE"
     gene            complement(10228..11460)
                     /locus_tag="PKX09_07930"
     CDS             complement(10228..11460)
                     /locus_tag="PKX09_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17788.1"
                     /translation="MGRAILIGVIILTVTFTAITVGVQKRVSNIPDQITESIDFMNSK
                     NLRDFALTYSLKKAYELDDDEDWHLAGFLSERLNVDFSDKLSDFFGGTIDSIRYTALN
                     PAGDSIRISSFITQHIGKGVKDFKTEAMVKFEIEEHPKSEIFGFDFDESDPDLVFDGS
                     NNNNNGILGNGVTNYKPTRTVGKNGSALDFDGDNDYVYVGENLIEPDGDELTVGTWVN
                     FSNTNTDWGILAAEKKSDSDHNPVWSVRARKTAGLKIFGFEIRPATIKVAFDIYNGVG
                     SYGYTEVDISKTEHEIDMNSWHYVVATYREVGDVTALISISIADLPVNNKETKLTTKW
                     QGRTDESYVTVGGDIYQSTYTGFLANLLNEVINRLNCTDTKLDEVKILNEALDDDDIQ
                     DLVNLGGIKDLRLAYWIQ"
     gene            complement(11500..12075)
                     /locus_tag="PKX09_07935"
     CDS             complement(11500..12075)
                     /locus_tag="PKX09_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17789.1"
                     /translation="MTSLLDAIFATSIGGLFLITIFTTLFNLQASSQDTTFHVTLNNA
                     AEVQYQELDNCLGKVGAGVYSGQILVAEPDSFVFKTKYDLINDIMGSVINTISISLGD
                     STSQGIPIKIMQNGVPLPGSNVPLWLRDLNFSYYDIDENQITTPGDSLSYIRLLKTDL
                     QFAFETSYVGPGERNIITNLTFWKYFINFYL"
     gene            complement(12072..12491)
                     /locus_tag="PKX09_07940"
     CDS             complement(12072..12491)
                     /locus_tag="PKX09_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17790.1"
                     /translation="MGYPQMFALIGSIALFTSVVLTMNTSINNQRQIVYQSIYMNQAV
                     KLASKVFDKITVETKTDLIDFDDIAITYSGTDSLRIWGNDVWYIYNVSSHYCDSSGVD
                     AAGKTDFQRVDVKIWTNPFVILDTLKFNNIYYNIELV"
     gene            complement(12494..14299)
                     /locus_tag="PKX09_07945"
     CDS             complement(12494..14299)
                     /locus_tag="PKX09_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOU17791.1"
                     /translation="MTNDKQSTVVSGSRLGQILVEKGIISPEILEQALKIQNEGDASS
                     SKRLEDILVSNFKIDHDLVFGTLAELYAFRTYRVNPEKLDPAQIKHTKELLSKFPDEL
                     KNQLLYYKVLPYQVINGSRNKLLVLASNPTEKVVEKIPLLTEFKKYEVVYTPLKIVEE
                     LIDLISPRENEFLDLLREAVVEPEIGEEKQVTELDEYSLDEEINKSLLVNLYEGCLIE
                     AVRRRASDIHIVPYEKNSVDIYFRIDGRLQLWYRQDNVSPEAMAAVVKDRTIGVDRFE
                     RDTAQDGFFQRNVDDYLIRYRVSVLPIISEEYERRFESIVIRIIDDRNVIADLRQLGF
                     LPQAEQDFIKAISKSKGIVILTGPTGSGKSTTLLAALHHVMDASKNILTIEDPVEYAI
                     KGARQLKIGRKLSFEDAIRTILRHDPDIVMVGEIRDKITADVAVKLANTGHLTFTTLH
                     TNDAPSAISRLYKMGVEPFLLAYAINIIVAQRLVRKLCPFCRVPISSEKYPAALELGL
                     TEEDLKSGKIFDAGTGCKKCNGGYKGRISLAEALYFSPEVRKSILNSGAEVDEAEIRE
                     IAEGQGMLSILDSGLEKIREGVTTVSEVSYAASEG"
BASE COUNT         4181 a         3142 c         2478 g         4714 t
ORIGIN      
        1 gggtctgtag caatacatcc ttcaccaaag taactttttc cggtaccatt tttaatatcc
       61 gaaaaagtcg cggagatact tccagttcct tcaatataaa ttggatcggg tagatattca
      121 ctccagataa tcgagtttac gatcgtaatc tttgaggcat attgacaggc aatatcgtta
      181 ccattcgatt tggcggtgtt tttggcaata gtaacattac gcaagaccgg gttagaatta
      241 ttgaaacagc agataccgcc gcccgttcca ccagattcgt tggctttatt attatagata
      301 acgactcttt ccaaagttgg gctggacgaa tagcaatgga ttcccgcgcc actatatttg
      361 gctacattat cgcggatgac cacatttttt atcgtagggc tggaattata acaataaata
      421 ccaccgccat agtcagcact tccattggtg acagtgaacc caatcaatac tgaacttgaa
      481 tcttcgccaa gactaaattt aaccacagtt ccagtagctt ggccatctat gattgttttt
      541 tggatgtggt cagttttgcc atcattataa aaaagtgagg cgactgtgat cttcttgcct
      601 ttgaagtcaa tcttctcctt gtaagtcccc tcggctacca gtataacatc tcccgctgcc
      661 gccacatcta tcccagcctg aatgcttgac tgatcatctg gaacctttat agttgctccg
      721 aaaaccaagc cggaaaatac caaaaaaatt aaactagcgc taaaaatttt ctttgccatt
      781 cttcacctcc attttcttat aatataaacc ccttttttat aaaaaatagc tttttctaac
      841 aaaatattta cgccccaatt gaaggttttt ataagattca gcatagaacc gttaatacaa
      901 ggaactcctg ttatctaatc tcaatacttc ggggttgaga ttagtcctaa atactgtcgg
      961 caaaaatata tccgctatca ttttatcctt taaggggcaa tttgaatgat tttcaatccc
     1021 tcactatcgg taacaaaggc gtaactgcca tccacaaaaa ccgaattggg agtggttgac
     1081 atttcaaaac tacctaagat aaaaggatta gccaaatccg aaatattaac cacgattaag
     1141 cctttttcct tatccgctaa ataagcataa ttgccacgca cgtagatact ataagcaaaa
     1201 ccgtgggtgg aacagtttga caccaaggac ggtgcttcta cactcgatac atcaaccatc
     1261 actaatccat tttcaccgtc agcaagaaac gcataattat ctttaataaa cacatcttga
     1321 gtaatactcg agctgggaaa atccgcaaca tatatcggat tcttaacatc ggaaacatca
     1381 aatattgtca agcctcgcga accgtaagcc acataagcat aaccattagc tacaaacaat
     1441 tttttggctt tttcatcagc cgagttattg ggaggtgtaa tatttgaaat tatatgtggt
     1501 gtactactca gaattgaagc gatggacata cccttctctt cttcacaaag ataagcatat
     1561 ggcggttcac ccgttacaaa aacatcaact ggaactccac ttgtctggca attaccgatt
     1621 aattccagcg tagtaacatc aacaatattc aagccaccag tgtgattagt agcataggct
     1681 aaattattat agatataaat tgacaatgtg cgatcgggta aaggtaaaac caaattatta
     1741 tcggtagagg gtgaggatgg atttatcaaa tctatgacta ttaaaccatt gtaatgatca
     1801 gcgatgtagg ctttccaatc cgagacaaat acttcagtgg cagtaccttt agtgactagg
     1861 gaactgacta agtttaaccg tatgttaata tttccctcat cagtaggttc tgaaaaatgt
     1921 tcacctttaa aagctttcac ccgatagtaa aaagttccac aggattcaga aatattatcc
     1981 aaaaacgttg tttggttgct atctaccgaa gcatatccat ttacccaatc ggacatgcca
     2041 acttttcgat cgatataaaa accggattca ccttgactat tatcctgcca gctaattttc
     2101 acaacatcct catcaatttt cgttaaggtt aaattagaag gcgccggaaa ggtattgtct
     2161 atttcatcgg tagtaacata gctggaaata tactcttctg aaaatccata tacccggtac
     2221 tggagtgtct cattgatttc ggcattctca tcaatccacc cggtttggtt cgaatcgagc
     2281 gttgctatct caacctgcca atctgcacta ccgacctttt tatctatttt aaagcctgcg
     2341 acattggatg ttttactttg ccaggtcagt ttgattttat ctatagcaat tcgttcatat
     2401 tcaagattat agggaggcgg aaacgtagca acgccaaaac cttccgcact gcccgacact
     2461 gatatgccgc agaaagcata gactcgataa aaaaccgaat cgccgaagga agttttatcg
     2521 gtaaaggaag tcccatttct gcctaaggtt ttataatctt taatccaatt cccgccgcta
     2581 actttcttct caattttata accatcttcg ccaatacagt tatcccacca agtcacctca
     2641 accttatcag attcgaggac cgaaatagct agttctgtag gaattgtttt tggtgaaaaa
     2701 taggctacca cgtcggaaaa ttccgatgcc aattccgatg tatcactttc ggtcttgatc
     2761 ttataggcat agattaggct atcattagtg gggatttcat ctatcaaaaa attatcataa
     2821 gtctgagcat attcgagatt ccagttagca tctcctactt ttctggcaac ggtaaaaaaa
     2881 acgtcctggt cagattggtc ataatcccag ctgacttcga tattattatc gtcgatggtc
     2941 ttgattctgg gattttcggg aatttcgatc ccaccgggtg aagtaatttt gccacagtcg
     3001 aaaatgacca ctaatgaaat cgctaaaaaa agaaatagtc ctgtaatttt taatttattc
     3061 ttcatccaat caccccaatc tcagataatt gacactccct aattaaatta ttgccaatag
     3121 taattattca ttcttgcact tttttgaaca gcagggtatc aatatgttct tttggtttat
     3181 agtatttctc gattaattct ctagctgatt ctttcttcgc cattcttact tcgataggtt
     3241 caatgagctc tgcttttagg ataattataa tttctctttc atttttctcg tgattactgt
     3301 aaccaaatat ataccgcaat cccaaaaccc accagggcag gtcttttaat attggaactc
     3361 ctttgcgaac aacaatatct tccgtgtcat aaagcccggc aactacggtc tcttctccat
     3421 catataaaat agcttcggta gtggcttgat ttttattaac gattacttgc acatccgaag
     3481 gtacaacact acttttttca actctggcaa ccaaatgtat ggcttcttta ctgctatctt
     3541 gaataatcgt tggagttacc tccagtataa gcccggcgga ataaaaccga tcaactaggt
     3601 tgccagaaaa atcggtggtt cgcacggaaa aatcttgacc aatctgcaaa tatccttgct
     3661 tcccactaat taccgtgaca ctaggtcggg cgataacggt acctttatta tagtgttcta
     3721 tcatcttgaa gagtgcattt aattcaataa cagcttctcc cgattctaag gtagtggaca
     3781 ccgttcccga aattatatca ctaagcactt ccgccccacc cgtaaaatcc agtgaggcaa
     3841 cgacagtacc gttataaatc gttgaccaat caataccaag gctattgata atcgacctgt
     3901 cggcagtaaa aaatatcgag ctgatcctga cttgtttggt atcgggaacg acctcatcgg
     3961 ccggtttttt ttcttcaacc gctttctctt ccgccataaa atcctttacc acatacgctc
     4021 ccgggagttc ctccactact aaccggttat aattggcaat taaatttaag gcgtttttcc
     4081 agtaaatctg agtaatagga atgctaatcg gtccggtata cgtgctcata ttcacgatct
     4141 tacgcccttc gtatttctga gccagcgctt cgatcatttt gagagcatcg gctaccagta
     4201 cttcgggtga aatagtaatc agttcatcat tagaaggact tttaatgaga tttacctgag
     4261 agttagttga actgaaaaac aacaatagac aaataaataa tgctttcttt ttcatatctt
     4321 atcctttccc ctgcaggtag atagtttttt ctacataaac accaattcta tttattttaa
     4381 aggtaactgc ttggttttcc caatcgattt ttgataaata gccataagct actttttcgc
     4441 cagttgtcag gcttatggtg cggccgttct tatccttaaa aaaggcaata gttggagtta
     4501 atcctactag gattgcttct tctggattta aatactgttc ttcttccttt atttgcactg
     4561 ggtcatggat cccgggataa aaaggattaa acctaatgtt gaccgtttta atctctctta
     4621 tcgggatact ttcaggctca atcccgttct catcataaaa tgcacgtaat ttgaatgcga
     4681 agggaattat atcctcacta gctggctgtt ctcttatttc taataactcc aacagataaa
     4741 gtttgggttg attctctatt tgatagataa aagtatataa agaattaaag ggaacaatcc
     4801 cagcaattaa ataatcatta tactttgttc cttgaatctc gccactttcg gccggtttga
     4861 aatcaaaaac catttccgga caaaatttct ggcatatctg cgtaagataa tcgtaagtaa
     4921 tcgtcggatt atcctctttc acaatggctt ttttaaattt agatatttcg atttctaatt
     4981 ccctgatttc ttgctctagt tcctcttctt caccaatatt ttccagttgg ctattgagca
     5041 tattcaggcg ttttgaaagt tcctgatttt tcttacttaa atcggcaatt tttctctctg
     5101 agctggaatt tgaaatgaca cctcctatta gtactatcag taagatcact gccaaggcta
     5161 ttgtatttct tattttatag ggcatattaa tgttcggtta cgtattttat tttttcctca
     5221 gccatttttg ctaatttatg ttctggatac tttgtgatta gcattcccca acagtcgatg
     5281 gctttggctt tatcaccgag tgcgttgtaa catctcccca acatcaacag ggcatccgga
     5341 gttttagtcc cgtcctggtt agcaattttt tcaaaaatat ctttcgcctg agctacttca
     5401 cccatcagga acagacactc gccaattaaa taattagcac tataagtaaa gggactattc
     5461 ggatatttct ctacaaactg ggtgagcttg tgataacctt cttgtatatt acccgataga
     5521 taggtctccg ccgcttcgcg gtaaacagta taaggagtaa tccttggggt agattgaact
     5581 ggtggtgact gactttgaac aacggcaggc gcaggtgcgg ctaatttggg agcgctttca
     5641 ctctcaattg attttggcgc tggagttatg gggggcggag cggcggtttt tccggattgc
     5701 ggagtaactg gagctggttt agcgactttc attacccatt cctcctgatc agagtacgaa
     5761 aaattcatca caaatcgcat caccggtata gattggatct ccgaggtttt gacatcggaa
     5821 actatacatc ctggaaaata cctggtaata taggcgatat tctctttatg gttggtagtg
     5881 ccctggacca taaaaccgct agtatcactc ctaagataat taatccagct aatagggttt
     5941 ttagcgaagg catctgataa cgctattatg gtacgatgcc actgactctt attgcctgaa
     6001 atagtcttaa tcttatccat actttttcgc ttaccggtaa tctgctgaga gagttcatta
     6061 gctcgggcga acataagatt cttgtgtctt aactcggatt caatattata attctggcgt
     6121 tgaagttcta caatctggcg gttgttttcc agatttttta gggtagcgga ataggtgaaa
     6181 taaaatattg caataaacag gataaagccg tgccagccga ttttaaatcg tttttgactc
     6241 tcgataattt tgggcggtag aagattagta ttatagaaag ctttattttt aggatcggcg
     6301 gcttttaatg ccaatgagat tggtatggca tattttgcga tgttttccgg agaatatttt
     6361 ctgtttatag tagcgatatt gatattttta aattcgaacc ggctaacctc ggtcgtttcg
     6421 tcgaatttac tactgaaaaa ccgtatatct tcatctcgga ccatattacc ggtcaagatc
     6481 aaatgctgaa ccggtggaat atcggaaacg tcctgctcaa ggataatctt ggaataaatc
     6541 acctgtcgga tgttttccga cctagaactg gtatcgccga taatcttaaa ggttttaacg
     6601 tgggtattat ttcgcatcac tatgcccgct ttatactcgg aacccacata caagatcaag
     6661 acgtagtcgt tctcggggaa attatagtta gctctaatca aattcatcaa ggcaatttca
     6721 ttggtatcaa tgtaactaaa aatgtatctc tttggggata atagcggtct tacctggaga
     6781 attgcattaa gtaaatcgaa ttcacctcga tgtaaaaatg ccagagtcat accgtcataa
     6841 ttcggtatgc atccaatggc atattttctg gatttcaatt cttcctggct taaaatttcg
     6901 gtctttattt tcttcttaat tgccgagaag gttaacgacg aattgaagat atggtaagat
     6961 atttgttcct cattcgcatt tagtgctatc tttccagttt ctaatgaaaa attgcttaaa
     7021 attcgctgca gttccgaagt agaagtcccc ccttctgcgt taacttcggt tatctcttcc
     7081 gtgagtttat tgctttcaaa ttcatcgaat tccgagattt ccggtaaatc aaatccttcc
     7141 tctttcagat cagggttatt ttgtatttcc aggttggtat cgcttaattc aatcgtgggg
     7201 atcttattta cctctttttt agtatagaga ggttcagata aagtcgtttc gcctaaattt
     7261 tggattttaa ttaaatcttc atccctcacg agatgagcta tttgtatatc taatccatcc
     7321 tggaaaatac catagtaatt tttcactttt tactcattta tgaagttcaa taaggttata
     7381 cacaaatttc ccgataggca agcaactgct ctattctttt tctattattg gagaagtcga
     7441 gagcagtttc tctacttata atttttttag tatatagatt atgaaggtct tgttccatcg
     7501 taaccattcc gtacttttta ccttcggtaa tcatctgata gatttcgcca atatttttgt
     7561 tcctaattgc ggcttttact gaatcagtga cagagagaat ttctttcact agggttaatt
     7621 tgttatctct atcaggaact aatttctgcg aaattactac gctaagggta tcagccaacc
     7681 tgagtctgat gcgctcttgt tcctcgggta aaaattcggc tataattcga tgaatacttt
     7741 caacggctga actggtgtgc aaagtggtaa atactttatg accggaatca gttgcttcca
     7801 gaaccgtagc aatcgtaggc gcatcacgca tctcgcccac gataattata tcggggtctt
     7861 ggcgtagagc ctggatcgtg ccttccctaa agcttagaac atcaccgcct acctcccggt
     7921 ggcggatgat acattttttg gatttatgga taaactcaag aggatcgcta ataattacga
     7981 tatggccttc attttcttca ttgttcaaat ttatgatgga atcgagagta gtgcttttcc
     8041 ctgaacccgt tatgccagtt attagaatta aaccggattt ctcatgccgc aaattaagcc
     8101 ttttcaaaac cggctcggga atattcaaat tctctatcgg aaaaagttcg cgatttactc
     8161 ttctgaaatt gcccaccaaa acgtcattat cgtaatagat gtcacctcta aaacggttgg
     8221 cttctttttc accaaaactt attcctagtg aaaaatcaat attcttttta tcgaaaagaa
     8281 taattttctg gtcatcggac aatatactga gcaaaatcgc cgtgatctca tcattggaat
     8341 aacgcttagc ttccaaatcg ggatatttcc tgccatatac tctataccag attttcacat
     8401 ccaacataga accgccaatg tctatatcgg aagcgtctat ctcttccatc ttttttaata
     8461 agctttttaa attatcataa gcttcttttt tccaggcgtt gttgaatgta attaacttat
     8521 taatattttt aaatctggct gtaccaatta gatactccgg taaggctatt tccatttcag
     8581 gtgtaaaaga taatcccatt tttacctcat agacttacgt aaatcggtca atttttatat
     8641 cctctattta agttcataca ttatatcaaa atggtagatg agtaaattca acctttattc
     8701 actctctgtg gttaccagct tcagcataat aattctctga attgatttcc tccacttgat
     8761 atataagcaa ttgattaggt taatcataaa ttttttgagc aacttttctc ccttgaaagc
     8821 aaagaattcg agtaattttt tacgctcaaa aaagcgggct gttaccgcgg tttccgctct
     8881 caagacactg gaagtaggac tattaataac gaaactctct tcgttccagg tatcccattt
     8941 cttcagataa tctactgagg tattacttaa ccaaaccgaa acttcgccac tttcaatcaa
     9001 aattattgaa ctagccattt tatcggcgat tcttatattc tccccaggtt caaaattgta
     9061 aattgagctg agtatcataa aatcttgctt gtcctcctcg gagaatccac ttaaaatagc
     9121 catttcacct ttttctacta gctcggtccc cggttcgaat attttcagga gaggcccata
     9181 taaatcctgc gagtatttta taccctcctt gaccgcatta agcagctcat caatattgat
     9241 tggttttgat aaatactgga atgcaccggc atggacggct ttaatagccc cttcgatact
     9301 ggaaaatccg gttagaatca ttacaaccgc caggggctgt ttttgcttta tcttcttcat
     9361 cagctccaat ccatccatct tcggcataac catatctacc agatataaat caaaatcatc
     9421 atagtccatt ttttccagcg cttcctcgcc atcagcggct aaggtaactt tatatccggc
     9481 ttcctcgagg atttccttgc agagcattct aatagtttga tcatcgtcaa cgacaagaat
     9541 atgactttgc ataattttct attctcgctt ctctcttttt tctttaatta aatctaattt
     9601 tttgagattt tgtaatctac tcaagatgcg ttttatatta tcaagtgctt ctgaaacctg
     9661 ttgactgctc cgcaataatt tctcgtcatt atattgttgc gccgcatttt gcaatcggcg
     9721 ttctaccagg gcaaaaacca tcaattggtt attgatgtca tggtcaatac tcgcgatgga
     9781 agcggctact tcaaccactt cctcatactt acgaatcaac tcttcctgat cactcatata
     9841 atttttccct tattttattc ttgcatcatt attccaatta caacactcac cttatagtgt
     9901 agcagtgaag aatcaaatta gaaaaacaac ctccgtcaat ttctgtctgg cttctgacca
     9961 aaatcatctg tttccgatca aacaacatca gcgaataaac ttacatttta acgttcaaaa
    10021 ctgctctcaa gccattctca tttcgactca atcccaccgt aacctttagt cttgatcaat
    10081 gaccccctgt gaaatacttg cttcgctatg ctgcaaattt tacggggcat accaatgact
    10141 aagagccatt tggggtaccc gagatggctt tctactactt aattttcaaa aaacatcata
    10201 tctcttaacg acagtgtccg atagttctca ttgaatccaa taggctaatc tcagatcctt
    10261 tattcctccg agattaacaa gatcttgaat atcgtcatca tccagagctt cattcagaat
    10321 cttgacctca tccagcttag tatcggtaca atttaatcta ttaataacct catttagtaa
    10381 attggctaaa aaaccagtat aagtactctg ataaatatca cctccaacag taacatagct
    10441 ctcatcggtg cgtccctgcc atttcgtagt taatttagtt tccttattat taaccggcag
    10501 gtcagcaatt gaaatagaaa taagcgccgt aacatcccca acttcacgat aggttgctac
    10561 cacataatgc caactattca tatctatctc atgttcagtt ttgctaatgt caacttcggt
    10621 atagccatac gatcctacgc cattataaat atcgaaagca actttaatag ttgcagggcg
    10681 gatctcaaag ccaaaaattt taagtcctgc ggttttacga gctcttacac tccatacagg
    10741 attatggtca ctatcactct ttttctcagc tgccagaatt ccccaatccg tattagtatt
    10801 ggaaaaattt acccatgtcc cgacggtaag ttcatcgcca tccggctcaa ttaagttttc
    10861 tcctacatac acataatcat tatcaccatc aaaatcaagc gctgagccat ttttgccaac
    10921 ggttctggta ggtttataat tggtaacacc attgcctaaa attccattat tgttattgtt
    10981 tgagccatca aaaaccaggt caggatcact ttcatcaaaa tcaaagccga atatttctga
    11041 tttaggatgt tcctctattt cgaatttcac catcgcttcg gtcttaaaat cctttactcc
    11101 tttcccgatg tgctgagtaa taaaagacga tatccgaata ctatctccag ccggattaag
    11161 agcagtataa cgaatgctat caattgtacc accaaaaaaa tcagataact tatcactgaa
    11221 atctacgttt aatctttcac ttaaaaatcc tgccagatgc caatcttcat cgtcatccaa
    11281 ttcgtatgcc tttttcaacg aatatgtcag cgcaaaatcg cgcagatttt tagaattcat
    11341 aaaatctata ctctcggtta tctggtcagg aatattacta actcgctttt gcactcctac
    11401 cgtaatcgcg gtgaaggtca cggtaagaat aattactcca attaaaattg ccctgcccat
    11461 tttaatacct cctcataagt tttttacaat ttatttccat cataaataaa aatttataaa
    11521 atatttccaa aaagtcaaat tggttataat attcctttcg ccagggccaa cataactggt
    11581 ttcaaacgca aattgcagat cagtttttaa taaacggatg taactcaacg aatctcccgg
    11641 agtagtaatt tgattctcat caatatcata gtaggaaaaa ttcaaatctc tcagccataa
    11701 tggaacattt gagccgggca aaggcacccc attttgcatt attttaatcg gaatcccctg
    11761 gctggtgcta tcgcctaatg aaatgctaat ggtattaatt accgaaccca ttatatcatt
    11821 gattaaatca tacttagttt tgaatacaaa actgtccggc tcggcgacta aaatttgtcc
    11881 gctataaacc cctgctccta cttttcccag gcaattatcg agctcctgat attgaacttc
    11941 tgccgcgttg ttgagcgtga catggaaagt ggtgtcttgc gaagatgcct gcaaattaaa
    12001 cagggtggta aagatcgtta tcaaaaataa accgccgata cttgtcgcaa atatggcatc
    12061 taataaactg gtcatactaa ttctatatta taataaatat tattaaactt tagagtatca
    12121 aggattacaa aaggattagt ccagattttt acatctactc tctggaaatc ggttttgccg
    12181 gcggcatcca ccccgctgga atcacaatag tggctactaa cattataaat ataccagacg
    12241 tcgttacccc agattcgcag tgaatccgtg ccggaataag tgatggcaat atcgtcaaaa
    12301 tcaatcaaat ccgtctttgt ttcgacagtt attttatcaa agactttact ggctaatttt
    12361 accgcctgat tcatatagat tgattgataa acaatttgtc tttgattgtt gattgaggta
    12421 ttcatcgtta ataccaccga cgtaaaaagt gcaatcgaac ctatcagagc aaacatttga
    12481 ggataaccca tctctaaccc tccgatgcgg cataagaaac ttccgaaaca gtggttactc
    12541 cctctctgat cttttctaat cctgaatcaa ggatcgagag cataccctgc ccctcggcaa
    12601 tttctctaat ttcagcttcg tccacttcag ctcccgaatt taagattgac tttcgaactt
    12661 cagggctgaa atagagtgct tcggctaaac taattcgacc tttgtagccg ccattgcatt
    12721 tcttacatcc ggtaccggcg tcaaagattt tgcccgattt caggtcttct tctgttaaac
    12781 ccaattctag cgcggctggg tacttttctg aagatatagg tacgcggcaa aaaggacaga
    12841 gcttgcgcac taatctctga gccacgataa tgttaatggc gtaagccagc aaaaacggtt
    12901 cgacacccat tttatataag cgcgagatag cgctgggagc atcattcgta tgcagggtag
    12961 taaaagtcaa atgaccggta ttggcaagtt taaccgctac gtccgccgta attttgtctc
    13021 taatttcacc taccatcaca atatctggat cgtgccgcag aatcgttctg atagcatctt
    13081 caaaacttaa ttttctacca atctttaact gccttgctcc tttgatagca tactctaccg
    13141 ggtcttcgat ggtgagaatg tttttcgacg catccataac atgatgcagg gctgccagaa
    13201 gagtcgtact cttaccgcta ccggttggcc cggtaaggat tactatccct ttagatttac
    13261 ttattgcttt tataaaatct tgttcagcct ggggtaaaaa tcccaactgc cttaagtcgg
    13321 caattacatt tctatcatct ataatccgga taacaatgct ctcgaaacgc ctttcatatt
    13381 cttcagaaat gatgggcaaa accgaaaccc gatagcgaat taaatagtca tctacattcc
    13441 tctgaaaaaa accatcctga gcggtatctc tttcaaaacg atcaacccca atagttcgat
    13501 ctttgactac cgccgccatc gcctccggcg aaacattatc ctgcctatac cataactgta
    13561 atcttccatc aattctaaaa taaatgtcaa cgctgttctt ttcataagga actatatgaa
    13621 tatcactggc tctcctccgc acggcttcga tcaaacaacc ttcataaaga ttaaccagaa
    13681 gacttttatt gatttcctca tccagagaat attcgtctaa ttcagtaact tgtttctctt
    13741 cgcctatttc cggctcgact accgcctccc ttaaaaggtc taaaaattca ttttctcgcg
    13801 gcgatattag gtcaatcaat tcctcaacta ttttaagagg agtataaaca acttcatatt
    13861 ttttaaattc ggtcaatagc ggaatttttt caacgacctt ctccgtcgga ttcgaagcta
    13921 atactaataa tttattcctg gaaccattga ttacttgata aggcagaact ttgtaatata
    13981 gcagttgatt ttttagctca tccggaaatt tggataataa ttccttagtg tgctttattt
    14041 gagccggatc gagcttctcc ggatttactc ggtaagttcg gaaagcgtat aattctgcca
    14101 gcgtaccaaa caccaaatca tgatcaattt tgaaatttga gaccagtata tcttcaagtc
    14161 ttttcgaact agaagcatcc ccctcattct ggattttaag ggcttgttcc agaatttctg
    14221 ggctgattat gcccttctcg accagtattt gccccagtcg ggagccactt actactgttg
    14281 attgcttgtc gttagtcatt ggtcattagt cattagtcat tggttttccg gtgaaaatgg
    14341 tgagggtagt gaagtgagta tttcactgga atcatttgta gttggtcggt taatgctgga
    14401 cttggattat cgttcattaa ttacagtcct tttttaataa tattcactaa tagtcctaat
    14461 atacatatta atttattttt tccaataaaa atattccttt aaactgaggc ggaga
//
LOCUS       DAOSMP010000047        14042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_4361_length_14042_cov_20.423679, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000047 DAOSMP010000000
VERSION     DAOSMP010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 14042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14042
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4361_length_14042_cov_20.423679"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="PKX09_07950"
     CDS             <1..129
                     /locus_tag="PKX09_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007043766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HOU17792.1"
                     /translation="CDCVRYHLRSRQLPACFFPDDVERTYDRSFEKFTELVQRGKV"
     gene            142..807
                     /locus_tag="PKX09_07955"
     CDS             142..807
                     /locus_tag="PKX09_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOU17793.1"
                     /translation="MKRHFYWLKLIICEALMFNLNNLAAESGEIRQLPKPDAVGRVPI
                     ETTLSQRRSVRTFQNEPLSEKEIAQLLYAAQGISDSSQGLRTAPSAGATYPLEIYLVT
                     AEGIFHYLPERHAIVLKKAGDCRDHLAEASLGQNSVRTAPAIIVFSAVYERTTRRYHK
                     RGERYVHIEVGHAAQNVHLQAVALGLASVPVGAFNDEAVARVIGIERNERVFYLVPVG
                     RKR"
     gene            976..2178
                     /gene="rocD"
                     /locus_tag="PKX09_07960"
     CDS             976..2178
                     /gene="rocD"
                     /locus_tag="PKX09_07960"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOU17794.1"
                     /translation="MLRSQDYIKIEDQYGAHNYQPLDVVLTRGQGIWLEDVEGKKYMD
                     FLAAYSAVNQGHCHPRLVKVMQEQCARLTLTSRAFRNDQWPLLAKELCELTGYEMVLP
                     MNSGAEAVETAIKAARKWGYLYKGVARDKAEIIVCTGNFHGRTTTIISFSTDEQYKDG
                     FGPLTPGFVAIEYGSIEELKRAINPNTVAFLVEPIQGESGVIVPPEGWLKASSEICRK
                     NKVLLIADEIQSGLGRTGKLFACEHENVRPDVVIIGKALSGGMYPVSAVVASQEILGV
                     FKPGDHGSTYGGNPLGSAIAREALKIIIDEKLPQKSAESGAYLMRKLKEIESPYIEQI
                     RGKGLWVGIVLKPSAGGARRFCVALQERGILAKETHENVIRLAPPLNITRSELDQAIA
                     ILQEVLTQ"
     gene            2229..3686
                     /locus_tag="PKX09_07965"
     CDS             2229..3686
                     /locus_tag="PKX09_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOU17795.1"
                     /translation="MKEAIEGLKPASLWRYFSEMSQIPRESKHEEAVIAWLESVAKKL
                     NLPYRKDEVGNIVISKPASPGYENRTPVVIQGHVDMVCEKNSGTIHDFRKDPIELVRD
                     GEYIRANGTTLGADNGIGVAAALAILEDKTLAHPPLEALFTIDEETGLTGANSLKPGF
                     IKGKILLNCDSEEDGALYVGCAGGKQTDLFFDINWEKAPQQMANVALKISGLKGGHSG
                     LDIDQGRANAIKQLNRIVWKAAEKYDFRLYYLEGGSKHNAIPREAVYKLLIKPADLAN
                     FETEIARFENILNNEYKDVERGIKLTVENLNEYPKQVFTKEFQARVLNCLYAIPHGVK
                     RMSPTIKGLVQTSNNAAIVSTGDKQIRVLTSQRSSVASEIEGLADKVKACGLLANAEV
                     VQSGGYPSWTPNMDSKILTLMKKVYADLFGVEPQVKAIHAGLECGIIGATSPGMDMIS
                     FGPTIEGAHSPDERVEIKTVEKFWRLLVETLKRIE"
     gene            4118..4927
                     /gene="uppP"
                     /locus_tag="PKX09_07970"
     CDS             4118..4927
                     /gene="uppP"
                     /locus_tag="PKX09_07970"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HOU17796.1"
                     /translation="MNYFESFILGIVQGLTEFLPISSSGHLVIVQHLFGLATDNIAFE
                     VMVHAGTLLSVLAVYYNDVWRMIVSFCRGIFRLRKAQPFQNDSYFRLSIFVIVATIPA
                     VIFGLTLDDFFEQIFHSVFLVGITLTITGLILLSTRWVKTEGRRLTFGKSLLIGCAQA
                     VAILPGISRSGSTISGGLWCGLDRMEATRFSFLLSVPAILGALVLHLKDLIGMALNPN
                     MIGILILGFLTSFIVGYIAIRFLLRVVQSDKFSWFGIYCIIVGLTVVFFLE"
     gene            4966..5937
                     /gene="holA"
                     /locus_tag="PKX09_07975"
     CDS             4966..5937
                     /gene="holA"
                     /locus_tag="PKX09_07975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOU17797.1"
                     /translation="MSKSDFLSELARIEQGHLDAVYLLRGGDLYLEDEAINTICQAFG
                     KQESERPEKLIYYGGVDSDLEFIDNLFSMGFFARRRIIIYKNIGNLEPNLRARLFNFI
                     ENPGQDTVLIMTADGESHSSLIEQLRKESRKVKTISTWTPKPSRFDEFIRRHLQKSQY
                     SIRPEALKLLIELTDDALSHTIGELEKLLIYIGERKMIEEDDVRLIVGGDKNYDMINF
                     LEAVAHRDITKAIKIGLALIQADASIPYFTTQLYDLFSGIWDYEGHGQKEKFWKKSNC
                     YELGIKNYKQADFGAIFTCIRNVDLQSKSLNLNKEDLLIQLLYEIIS"
     gene            5951..6532
                     /locus_tag="PKX09_07980"
     CDS             5951..6532
                     /locus_tag="PKX09_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU17798.1"
                     /translation="MNPEFQLTDEELISRFQAGDEGAFEEIVNRYADRLLNFAYRFVL
                     DREEAEDIVQDTFLKVYQNRHAYREIAKFSTWIYTITGNLAKTILRKRKNRKMFPFSR
                     TASEDKEREIEYPSTERPPDTKLAGEIEEQIIQRAIIKLPEHFRTVIILRDIQDLSYE
                     EISNIIDAPLGTVKSRINRARLRLQEELEHLRR"
     gene            6722..7183
                     /locus_tag="PKX09_07985"
     CDS             6722..7183
                     /locus_tag="PKX09_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17799.1"
                     /translation="MLSLPKISVSDDFKLNLRNRILSDRSAATQPNSHKGFNLSRIPS
                     FAYGFAVAVVAVVVGFFILQSQSQENSPDNMPPIVRQKMTQSPAQQPQDVTPNNPNQS
                     GLQSPQYTSKQNQPLPSDTTTSNNSSSQSAEPTFEYEPNYEDQIKTVKDQR"
     gene            7263..9029
                     /locus_tag="PKX09_07990"
     CDS             7263..9029
                     /locus_tag="PKX09_07990"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOU17800.1"
                     /translation="MKNKSIIIISIILFTILFLAFVFPRLEFFSGIPVIVQTIFRILI
                     YIGVLSYLIFIIQELYHWRSRCDLAEKTLDSRRSSISRDDEFAGELSLKLKIDPDQNY
                     NEIIAQILKIAQSSLMARSAFLYLFNADEHTYILQAYQSQEGIEFRPKFKAEGTIFRE
                     LHLTLKPQIYSADGLKEGSLVYYTVPPKVGTIMVVPIMFNQEVFVGVLGLDSIDRDAW
                     GHEDVDLVKLFAELFSSSIWQIDAIDRLNTQIAFFKALCRLNTELPIGIESLELYKEA
                     SQVLHKFFNFDKLTFATLPEEESPELYIEYVEGYDTDYSIGHRISWKGGLWEKIIKNG
                     EILLLNDYEQEKLSFRFQPGDLGILPFRSCLGVPLEVGRKRLGGILMESYQPHNYLRD
                     ESDTLVLFAKNLSEIINRMNTYQTVKDLAMIDGLTGLYNRRAFEERLQIEIERSRRYE
                     NSLTLMILDLDKFKRINDTYGHIFGDMVLKKTASIIRGSVRTVDTVARYGGEEFAVIL
                     INADKNNSYKSAERIRSNIQSFVFEKDGIREHITISIGMAEYPMDGTDVPTMISHADL
                     AMYQSKRLGGNKVTLFKKEYAT"
     gene            9042..9245
                     /locus_tag="PKX09_07995"
     CDS             9042..9245
                     /locus_tag="PKX09_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17801.1"
                     /translation="MSKGVKGFADKVAKSSKDNKTYCPVCGESVEYVKHIETTSDPSN
                     KRSMRFKEQIVGVCKCNRDQIYQ"
     gene            9314..10831
                     /locus_tag="PKX09_08000"
     CDS             9314..10831
                     /locus_tag="PKX09_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU17802.1"
                     /translation="MKKLYPSRATTILRKIVGLLVLAALVSCAATKQTSKSAEKPAES
                     SKNTQYNSQALTHFMNGENYALQGDYAMAVLEYQDALLYETKSAAIYSSLGKAYINLG
                     KLERGLAALEKACQLDPDDAETREILAQIYFLTGRFQQAEKQYQALMTLRPNEPEIMY
                     QLAAIYLKNRKIDQALQLYDEIVKVDSTQYQALQKAAEIAILNRDLNKATAYYDQLVK
                     LFPDSLLYLKTRADLAALDNDFETAIDIYKKLIILNPGDIELRERLGEILCWSDHFEE
                     AQTILEAIIDANPERKASYANLALLYVRNENYQQALDILDSARIRFPNDPSFLLAQGS
                     IYNTLRDYQSAEKVLLKTLQIEPNNVQAQHLLAAIWDMLKKYSQCDSLYEVIIRHNPD
                     DVIALNNYAYSLAVRGERLDEALKMVDLALAKEPNNASYLDTKGWILYRQKKYKEAIK
                     YIQKSFEIEQDNAEVLEHLGDVYQALRQPKKAHIYYQRAAEIDKNNENLKRKLQP"
     gene            10800..11528
                     /locus_tag="PKX09_08005"
     CDS             10800..11528
                     /locus_tag="PKX09_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17803.1"
                     /translation="MKTLSVNCNRKFTGLLISLILSFSGWNCSHAPLKHEKVDEHWAP
                     ALRERSRQLKQLANLSGSGQVSFSGTAGSGIIYFDFEYRTADTLNLWIKDPIGRKLAN
                     LELGGDTYHLHLIRDGRYFSGTQLETDQLPISCGPITPALIRRILLAVPFESTTNETA
                     SQFNEASDETPVLYYKFKPSQGEPAELNWLYKNKQIIIVYKEYTDVARLKIPAQIVIK
                     NKAEEIAVEIHFSHFSAEMLKFRT"
     gene            11554..12480
                     /locus_tag="PKX09_08010"
     CDS             11554..12480
                     /locus_tag="PKX09_08010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU17804.1"
                     /translation="MNSENEKKLVTVVIPVFNEVESLRELIEKIQAAFRPKNYNLEII
                     IVDDGSRDGSAQVAREIASTFPNVTVTGFQRNRGKAAALNEGFRLAKGDFVATIDADL
                     QDDPEAIPAMLDYLQESGYDLVSGWKQKRQDKFVKKTTSKIFNSVTRLLTGVKLHDIN
                     NGLKVYRAEVVKSIYLYGELHRFIPVLAKFEGFTSGEYKVKHFPRKYGKTKYGVSRFY
                     KGFLDLLTVIFITKFMRRPMHFFGLWGMVIVLIGLLSELWVLILKFGFQEPFKDHVAL
                     LIFGVLAIVFGMQFFSLGLISEMITFQNRKEK"
     gene            12480..>12621
                     /locus_tag="PKX09_08015"
     CDS             12480..>12621
                     /locus_tag="PKX09_08015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU17805.1"
                     /translation="MPKYRFSVIIPSYNRADEVIDLLKSLQAQTCEHSRFEVLVIDDG
                     STD"
     assembly_gap    12622..12721
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12722..13303
                     /locus_tag="PKX09_08020"
     CDS             <12722..13303
                     /locus_tag="PKX09_08020"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU17806.1"
                     /translation="HSQKRLAKFYPRSFNMGIRREIYEKLGGMGNLRHGQDIEFSRRI
                     VASGAKVIYIPNAIVYHKRRTSLRKFFRQMFNWGVARINLSYLDAGNLEPLHFIPAIA
                     AVGYTLLTIFTLLTGLAWKIWVAITVLALLICGYIFIDSSIKYKSVKVGWLAPIFAVC
                     QIFAYGYGFTYNYVRRKIFRRGEVTGFVRRYYQ"
     gene            13300..>14042
                     /locus_tag="PKX09_08025"
     CDS             13300..>14042
                     /locus_tag="PKX09_08025"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU17807.1"
                     /translation="MTNISPRNLNFHKEQYWTAANFLEKYINISPVSGLRILEVGSAE
                     GGGLNYFATKNNQCYGLEYSRGRLEIARELNKSSKIIFIHGDITEASSYENQLQDKMD
                     LIICGDVIEHIANEKKAAALQNMRDLLQKGGRIYISFPPKYSPFAGHQQGLPGWLHRI
                     PYLFLLPDKFYSAILKSQGIRADIISNMLATKHSRLSIKQFEKMVYENGLRILKKDFF
                     LIRPRFKYNYGIPYIGHRCDLPIVREIFNL"
BASE COUNT         4051 a         2795 c         3134 g         3962 t
ORIGIN      
        1 tgtgattgcg ttcgttatca tctgcggtcg aggcaactac cggcttgttt tttccccgat
       61 gatgttgagc gcacttatga tcgctctttt gaaaaattca ctgaattagt ccaaagagga
      121 aaagtatagc cgataaaaga aatgaaacgt catttttact ggttaaaatt aattatttgc
      181 gaggctttga tgttcaattt aaacaattta gcggctgaat ccggagaaat aaggcaactt
      241 cccaagcctg atgcagtcgg tagagtccca attgagacga ctttgagcca acgccgttcg
      301 gtgcgaacat tccagaatga gccgttgtcc gaaaaggaaa ttgcccagtt gctttatgcg
      361 gcgcagggaa tcagcgactc ttcacaggga ttgcggacgg cgccctcggc cggggcgaca
      421 tacccgctgg aaatttatct cgtgacggcg gagggaattt ttcactattt gccggaacgc
      481 catgccatcg tattaaaaaa agccggtgat tgtcgcgatc atttagccga agccagtctg
      541 ggacagaatt ccgttcgtac tgcaccagcc atcatcgttt ttagtgcagt ttatgagcgt
      601 acgacgcgcc gttatcataa gcgtggtgag cgctacgtcc atattgaagt tggtcatgcc
      661 gctcaaaatg ttcatctaca ggcagtggcg ctcggactgg cttctgtgcc ggtgggagcc
      721 tttaatgacg aggcagtcgc cagagtcatt ggtattgaac ggaatgaacg agtgttttat
      781 ctagtgccgg ttggccgaaa gcgttgagtc cggcatattt tattaagaaa cgtcatcgtc
      841 cgataattaa tgaggcaaat ttatcctatt tacggataag ttttcagtca gctataagaa
      901 aaccgatgcc tttctactct taattggcta aatttcacct aatttttgat atttcaaaaa
      961 attggagtct aaagaatgtt acgtagtcag gattatatta agatcgaaga tcagtatggt
     1021 gctcataatt accagccgct ggacgtagtt ttgacccgcg gtcagggaat ctggctcgag
     1081 gatgtcgaag gcaaaaagta tatggatttt ctggcggctt actcggcagt caatcagggg
     1141 cattgtcatc cacgtctggt caaagttatg caagagcaat gtgcccgtct gacgctgacc
     1201 tcgcgcgctt ttcgtaatga ccaatggccg ctactagcca aagaactttg tgaactaact
     1261 ggttacgaaa tggtattacc gatgaactcc ggggcggaag cagttgaaac agcgatcaaa
     1321 gccgcccgca aatggggtta tttatacaaa ggcgttgcca gagacaaagc tgaaatcatc
     1381 gtttgtacgg gcaattttca cggtcgcacc acgacgatta tttctttcag caccgatgaa
     1441 caatacaaag acggatttgg tccgctaacg cccggctttg tcgccataga atatggttcg
     1501 attgaagagt tgaaaagggc tatcaatccc aatacggttg cctttctggt cgaaccgatt
     1561 caaggcgaaa gcggtgttat cgtgccaccg gagggatggc taaaggcatc ttccgaaatt
     1621 tgtcgcaaaa ataaggtttt gttaattgcc gatgaaattc agagcggtct ggggcgcacg
     1681 ggtaaactct ttgcctgcga acatgaaaat gttcgaccgg acgtagtaat tattggcaaa
     1741 gcgctttccg gcggaatgta tcccgtctca gccgtagttg cttctcagga aattctgggg
     1801 gttttcaaac ccggcgatca tggctcaacc tatggtggaa atcccttagg cagtgccatt
     1861 gctcgcgaag ctctcaaaat cattattgac gagaaattgc cgcagaaatc cgctgaatcc
     1921 ggtgcttatt taatgcgaaa attaaaagaa attgaaagcc cgtatataga acaaattaga
     1981 ggcaaaggtt tgtgggtcgg catcgttttg aaaccatcag ccggaggcgc ccgtcgcttc
     2041 tgtgtggcat tgcaggagcg tggcattctg gcaaaagaga ctcatgaaaa cgtcattcgc
     2101 ctagcgccgc cgctaaatat tacccgaagt gaactcgacc aggcgatcgc aatattgcaa
     2161 gaagttttga ctcaataata tctgaaaaaa ctaaactcat aagagttaaa aactagcagg
     2221 aggtttattt gaaagaagca atcgaaggat taaaaccggc cagtctatgg cgctattttt
     2281 ctgaaatgag ccagattccg cgcgaatcca agcacgagga agccgtaatt gcctggctcg
     2341 aatcagttgc taaaaaattg aatcttccct atcgcaaaga cgaagtcggt aatattgtca
     2401 tttctaaacc ggcctctcct ggttatgaaa accgcacgcc ggtagttata caggggcatg
     2461 ttgatatggt ttgtgagaag aacagcggca ccatccatga tttccgaaaa gacccgatag
     2521 aattggttcg tgacggcgag tatataaggg ctaatggtac tactctcggt gccgataatg
     2581 gcatcggtgt agcggccgcc ctggcgattc ttgaggataa aactttagca cacccgccgc
     2641 tcgaagctct ttttaccatt gacgaggaaa ccggcttaac aggcgcaaac tccttaaagc
     2701 ctggatttat taagggcaaa atcctactga attgtgattc ggaagaagat ggcgctttgt
     2761 atgtcggttg tgccggtgga aaacaaaccg atctattttt cgatattaac tgggaaaaag
     2821 cgcctcaaca aatggctaat gttgccctaa aaatttccgg gctgaaaggc ggtcattccg
     2881 gactggatat tgaccagggg cgtgccaatg ctattaagca attgaaccgc atagtttgga
     2941 aagccgctga gaaatatgac tttcggcttt attacttgga aggcggcagt aaacataatg
     3001 ccattccgcg cgaagccgtt tataaattgc tgataaaacc ggctgattta gctaatttcg
     3061 agaccgagat tgccagattt gaaaatatat taaataacga gtataaagat gttgaacgtg
     3121 gcattaaact gacggttgaa aatttaaatg aatacccgaa gcaggttttc acgaaggaat
     3181 ttcaagcgcg agtattgaac tgcctctatg ccatcccgca cggtgtcaag agaatgagcc
     3241 ccacaatcaa gggattggtg cagacctcca ataatgccgc tatagtcagt actggtgata
     3301 agcagatcag agttcttacc agtcagagaa gttcggttgc ctccgaaatc gagggcttag
     3361 ctgataaagt caaagcttgt ggtcttttag ccaacgccga agtagttcaa agtggaggtt
     3421 atccgtcttg gactcctaat atggattcga agattctaac attgatgaaa aaggtttatg
     3481 ccgatctttt tggcgttgaa ccccaggtca aggctattca cgccggactg gaatgtggta
     3541 ttatcggggc tacttcaccg ggtatggata tgatttcatt tggtccaact atcgaggggg
     3601 cacattcgcc agacgaacgg gtcgaaatca agactgtcga gaaattctgg cggctgttgg
     3661 tcgagacact caaacgaatc gaataattgc tagattcgta tagattgaaa accaccacgc
     3721 aagctctaag acttaaggta tttaattttc tcttgaaagt aatttggaat gctccgataa
     3781 tgtcggagcg cctcaggata gagcgaggtg aacagtccag cctttaggtt gtgcgcataa
     3841 attgatactg tcgatttaat ccctactatc aagcataacg agaaagattg caacctttag
     3901 aaggtctgat aaccaattta ccaaaccgac cttcttcagg ttaacaaatg ctacttctac
     3961 cgatcagcct tggtaaaatg ttacattcat ttgatttggc tcaaattgat ttagataatt
     4021 tttggtcgag attaaaagat atttagtcat tatggattgg taaaacaatc ttggtaaacc
     4081 ggctagctcg gaataaattt actatatttg gaggtttatg aattactttg aatcttttat
     4141 tttaggaatt gtacaaggct tgacggagtt cctgccgatt agcagttcag gacatttggt
     4201 aatcgttcag catctctttg ggttagcgac cgacaatatt gcctttgaag ttatggttca
     4261 cgccggcact ttattgagtg tgctggcggt ttattacaat gacgtatggc ggatgatagt
     4321 aagtttttgt cgtggaattt tccggctacg caaagcacaa ccgtttcaga atgattctta
     4381 ttttcggctt agcatttttg taattgtggc tactattccg gcggtaatat tcggtttgac
     4441 attggacgat ttcttcgagc aaattttcca tagcgtattt ttagtcggca ttacgttgac
     4501 gattaccggt ttgattcttt tatcaacgcg gtgggtaaaa accgaaggcc gccgattgac
     4561 ttttggtaaa agtctcttga ttggttgtgc ccaggcggta gcgattttgc ccggtatttc
     4621 gcgtagcggt tcgacgatca gcggcggttt gtggtgtggc ttggatcgga tggaagccac
     4681 ccgtttttcg tttctgctct cggtgccggc aattctgggc gcattggttt tgcatctcaa
     4741 agatctgatt ggtatggctc tcaatcccaa tatgattggg atattaatcc ttggattttt
     4801 aacctctttt attgttggat atattgctat tcgattttta ctaagagtag tgcaaagtga
     4861 taaattcagc tggtttggta tctattgcat aatagtcgga ctgacagttg tatttttcct
     4921 cgaataaaag tttaactaat aagaagccca aggaatccaa tggcaatgtc gaagagtgat
     4981 ttcttaagtg aattagcgag aattgagcaa ggtcatctcg atgcggttta tttattacgg
     5041 ggtggtgatc tttatctgga ggacgaagcc atcaatacca tttgtcaggc ttttggcaaa
     5101 caagaatctg agaggccgga aaaactgatt tactacggcg gtgttgatag cgatctcgaa
     5161 ttcattgata atcttttcag tatgggattt tttgccaggc gccggattat tatctataaa
     5221 aatattggta atcttgagcc aaatctgcgt gctcggcttt ttaattttat cgaaaacccc
     5281 ggacaggata ctgttttaat tatgactgcc gatggcgaaa gtcattccag cctaattgaa
     5341 caattgcgta aagaatcgcg gaaggtcaaa acgatttcga cttggacgcc caaaccctcc
     5401 aggtttgatg aattcatccg acgccacctc caaaaaagtc aatattccat ccgaccggaa
     5461 gcgctcaaat tgctgataga attgaccgat gatgcgctca gccatacgat cggtgaattg
     5521 gaaaagttgc tgatttacat tggcgagcgg aaaatgatcg aagaagatga tgttcgtcta
     5581 attgttggcg gcgataagaa ttacgatatg attaatttcc ttgaagctgt agctcaccgc
     5641 gacataacca aagcaataaa gatcggtttg gctctaatcc aagctgatgc atcaatcccc
     5701 tattttacca cccaattgta tgacttattc agcggcattt gggattatga gggacatggt
     5761 caaaaagaaa aattctggaa gaaaagtaat tgttatgagc tgggaataaa gaactataaa
     5821 caggctgatt ttggtgcaat ttttacctgt attagaaatg tggatttaca atcaaaatct
     5881 ttaaatttga ataaagaaga tttattaatt caattgttgt atgaaatcat ctcctaagaa
     5941 aaaaacagaa atgaaccccg aatttcaact gaccgatgaa gaattgatca gccgttttca
     6001 agccggtgat gaaggcgctt tcgaagaaat tgtcaaccgt tatgccgacc gtttgttgaa
     6061 ttttgcctat cgtttcgtcc tggatcggga agaagcggag gatattgtcc aggatacttt
     6121 ccttaaagtt tatcaaaacc gccatgccta tcgtgaaata gccaaatttt ccacctggat
     6181 ttacactatt actggcaatt tggctaaaac cattctgcgc aagcgcaaga atcgcaaaat
     6241 gtttcccttt tcgcgtacag cttcagaaga taaagagaga gaaatcgagt acccctccac
     6301 cgaacgtccg ccggatacta aactggcagg cgagatcgag gaacaaataa ttcagcgggc
     6361 aattataaaa ttaccggaac attttcgtac cgtaatcatt ttaagggata ttcaagatct
     6421 ttcatatgag gaaattagca atatcattga cgcgccatta gggacggtca agtcacggat
     6481 caaccgggcg cggctgagat tgcaggaaga acttgaacat ttaaggcgat agagaagatg
     6541 attagtaaaa aatgggggaa atcaaaaatt tatgatgaat tgctaccaat ttaggaattc
     6601 catatcaaat tttattgacg aaaacatctc cttcaaaaac cgtcaagctt tccaagagca
     6661 cctgaatgaa tgcccagttt gccagaaaat ttatagctcc atcttggcga cccggcaaaa
     6721 tatgttaagc ttacctaaaa tttctgtaag tgacgatttc aaacttaacc tacgcaaccg
     6781 gattctttcc gatcgcagtg cggctactca gcctaactca cataagggtt ttaacttaag
     6841 ccgaattccg tcatttgctt atggctttgc cgtagcagtg gttgccgtgg tagtcgggtt
     6901 ttttattctg caatctcaga gtcaggaaaa ttcgccggac aatatgccgc cgatcgtacg
     6961 tcaaaaaatg acccaatctc cggctcaaca gcctcaagat gtgacgccga ataatccgaa
     7021 ccaatccggt ttacagtctc ctcaatatac ctctaaacaa aatcaaccgc ttccgagcga
     7081 taccactact tcgaataatt cttcgagcca atcagctgaa cccacgtttg aatatgagcc
     7141 aaattatgag gatcagatta aaacggtcaa ggatcaacgg tgagttgaca ggctttatta
     7201 tattaattgt tggttaaacc taatttttaa agtattttta tacccgttat gggtattttt
     7261 ttatgaaaaa taaaagcatc ataataatca gcataattct attcacgatc ctatttttag
     7321 cgtttgtttt tccgcggctg gaatttttca gcggaatacc ggtaatagtt caaactatct
     7381 ttcgaatact gatttatata ggagttctgt cttatttaat tttcattatt caggaattgt
     7441 accattggcg gagtcgctgt gatttagccg agaaaactct ggactcccgc cgatcttcca
     7501 tcagccggga tgacgaattt gctggtgagc ttagtctgaa gctgaaaatt gatccggatc
     7561 agaattacaa cgaaattatc gcccaaatat tgaagatagc ccaatcttct ttaatggcgc
     7621 gtagtgcatt cctctattta tttaatgccg atgaacatac ttatattttg caggcttatc
     7681 agtctcaaga aggaattgag tttcgtccca agttcaaggc tgaaggcact attttccggg
     7741 aattgcatct gactttaaaa ccacagatat actcagccga tggtcttaaa gaaggtagtt
     7801 tggtttatta tactgttccg cccaaagtcg gcacaatcat ggtcgttccc ataatgttca
     7861 atcaggaagt atttgtcggt gtccttgggc ttgatagcat tgaccgtgat gcctggggac
     7921 acgaagatgt tgatctggtg aaactctttg cggaattatt ttcctcctca atctggcaaa
     7981 ttgacgccat tgaccgactc aacactcaaa ttgccttttt caaagccctt tgccgcttga
     8041 acacggaatt acctattgga attgagagtc ttgaattata caaagaagcc agccaggttt
     8101 tacacaaatt ttttaatttt gataaattga ctttcgccac tttacctgag gaggaatcac
     8161 ccgaactcta tattgagtat gtcgaaggtt acgatactga ctatagtatc ggtcatcgca
     8221 tttcctggaa aggcggcctc tgggagaaaa tcatcaagaa tggtgagata ctcctgctga
     8281 acgattatga gcaggaaaaa ctatcatttc gttttcaacc gggagattta ggtattctgc
     8341 cctttcgttc ctgtctggga gtgccgttgg aagtagggcg caagcgactg ggtggcattc
     8401 tgatggaaag ctatcagccc cacaattatc tacgggatga aagcgatacc ctggttttat
     8461 ttgctaaaaa cctcagcgaa ataatcaatc ggatgaatac ttatcagacg gtgaaagatt
     8521 tagcgatgat tgatgggctt accggtctct ataatcgtcg agctttcgaa gaacgtctcc
     8581 agattgaaat cgagcgcagt cgtcgttatg aaaattccct gaccctgatg attctcgatt
     8641 tagataagtt caaaagaatc aatgacacgt acggacatat cttcggggat atggttttga
     8701 aaaaaacagc ctctattatt cgcggaagtg tccggacagt tgatacagtc gcgcgatatg
     8761 gtggtgaaga gttcgccgta attctaatta atgccgataa aaacaacagt tataaatcag
     8821 ctgaaagaat tcgcagtaat atccagagct ttgtatttga gaaagacgga attcgcgaac
     8881 atataacaat tagtatcggt atggcggaat atccgatgga cggcactgat gttccgacta
     8941 tgatttcgca tgccgattta gcaatgtatc aatcaaaacg tctgggtggc aataaagtaa
     9001 ccttatttaa gaaagaatat gcaacttaaa aggagtcaat tatgagtaaa ggtgtcaaag
     9061 gatttgccga taaagtagcc aaatcctcaa aagataataa aacttattgt ccggtttgtg
     9121 gggaaagcgt tgaatatgta aaacatattg agactacttc cgatccgtcc aataaaagat
     9181 caatgcgttt taaggagcag attgtcgggg tttgcaaatg caaccgcgat caaatttatc
     9241 aataaccata agaccactct cttgtcaagc cccgtttttt gggctggctt agttagagtg
     9301 ggaagtgaaa aacatgaaaa aactatatcc aagcagggcg acaactattc tcaggaaaat
     9361 agtcggtttg cttgtattag ccgctctggt tagttgtgcc gctaccaaac aaactagtaa
     9421 atctgcggaa aaaccggctg aatcatccaa aaatactcaa tataattctc aggctctgac
     9481 gcattttatg aatggtgaga attatgctct acagggcgat tatgctatgg cagtactgga
     9541 ataccaggat gcacttcttt acgaaactaa atcggcggct atctacagct ctctcggcaa
     9601 agcctatatt aatctcggca aactggaacg tggcttggcg gctctggaaa aagcctgcca
     9661 attggatccg gacgacgccg aaactcgcga aatcttagcc cagatttatt ttctgaccgg
     9721 gcgttttcag caggctgaaa aacagtacca ggctcttatg actttacgac ccaatgaacc
     9781 ggaaataatg tatcaattag ccgctattta tctcaagaac cgaaaaattg atcaggcttt
     9841 gcaactctac gatgaaattg tgaaagttga ttcgactcaa tatcaagccc tccagaaggc
     9901 ggcggagatc gctattctca atcgcgatct aaataaagcc accgcttatt atgatcaatt
     9961 ggtaaaacta tttcccgata gcttattgta tttaaaaacc cgagcggatt tagcagcatt
    10021 ggacaatgac tttgaaacag cgattgacat ctacaaaaaa ttgattattt taaatcccgg
    10081 cgatattgaa ttaagagaac gactcggaga aattttgtgc tggagcgacc attttgagga
    10141 agctcaaaca atcctcgaag caattatcga tgctaatcct gagcgcaaag ccagttacgc
    10201 caatctggca ttactttatg ttaggaatga aaattatcaa caggctcttg atattctcga
    10261 ttctgccaga atccgtttcc cgaatgatcc atcgttttta ctcgctcagg gttcgattta
    10321 caataccctg cgcgattatc aatcggcaga aaaggttctg ttgaagactt tacagattga
    10381 accaaataat gtccaagccc agcatctgct ggcggcaatc tgggatatgt taaaaaaata
    10441 ttcgcaatgt gatagccttt atgaagtaat tatacgccat aatcccgatg acgtcatcgc
    10501 cttgaacaat tatgcttaca gtttggcagt gcgtggtgaa cgactcgacg aagccttaaa
    10561 aatggtggat ttggctctgg ctaaagagcc caataatgcc tcttatctgg ataccaaagg
    10621 ctggattctc taccggcaga aaaaatataa agaagcaata aagtatattc agaaatcttt
    10681 cgaaattgag caggacaatg ccgaggtact ggaacatctt ggcgacgtct atcaagcctt
    10741 acggcaacct aaaaaagccc atatttacta ccaacgagcc gccgaaattg ataaaaataa
    10801 tgaaaacctt aagcgtaaac tgcaaccgta agttcacagg tttactaatt agcctgatct
    10861 tatcgtttag cggctggaat tgtagtcatg cacccctcaa acatgaaaaa gttgatgagc
    10921 attgggcgcc cgccttgaga gaacgcagtc ggcaattgaa acagctggct aatctttccg
    10981 gttctggcca ggtgagtttt agtggaacgg ctggtagcgg aataatctat tttgattttg
    11041 agtaccgaac cgcggataca ttgaatcttt ggatcaaaga cccgatcgga aggaagttgg
    11101 ctaatcttga attaggcggt gatacttatc atttacattt aatccgggat ggtaggtatt
    11161 ttagcggaac gcaattagaa actgaccaat taccaatttc ttgcggtcca atcactccgg
    11221 cattaatccg acgaatttta ctagccgtgc cttttgaatc taccaccaac gaaaccgctt
    11281 ctcaattcaa tgaggcaagt gatgaaacgc ctgttttgta ttataaattt aaacccagtc
    11341 agggtgaacc agctgaactt aattggcttt acaaaaacaa acagataatt attgtttata
    11401 aagaatatac tgatgttgct aggttaaaaa ttcctgctca aatagttatc aaaaacaagg
    11461 cggaagagat cgcagtagaa attcatttct cccatttcag cgccgagatg cttaaatttc
    11521 ggacgtaata tattcgccat tatcgaggta aatgtgaatt cggagaatga aaaaaaactt
    11581 gttacggtag tgatcccggt tttcaatgaa gtggaatcac tgcgcgaact gatcgaaaaa
    11641 attcaagccg ccttccgtcc aaagaactat aacctggaaa taatcattgt tgacgacggc
    11701 agtcgcgacg gctcggcaca agttgccagg gaaatcgcca gcacttttcc caatgtaacg
    11761 gttaccggtt ttcaacgtaa tcgtggtaaa gccgccgccc tgaatgaggg tttccgtctg
    11821 gcaaaaggtg acttcgtggc tactattgat gccgatttac aggacgatcc ggaagccatt
    11881 ccggcaatgc tggattatct ccaggaatca ggctatgatt tggtatctgg ctggaaacaa
    11941 aagcgccagg acaaatttgt taagaaaaca acctcgaaaa tttttaattc agtcactcgc
    12001 ttgttaaccg gtgtgaagct gcacgatatc aataatggcc taaaagttta ccgtgccgag
    12061 gtcgtcaaat ccatctatct gtatggcgaa ttgcatcggt ttatcccggt attggctaaa
    12121 tttgagggat tcaccagcgg cgagtataaa gtcaaacatt ttccacgtaa atatggcaaa
    12181 acaaaatacg gtgtcagccg gttttataaa ggtttcctcg atttattaac ggtgatcttt
    12241 attactaaat ttatgcgccg accgatgcac ttcttcggct tatggggaat ggtaattgtg
    12301 ttgattggct tgctatcgga attatgggtt ttgatcctaa aattcggatt tcaggagcca
    12361 ttcaaagatc acgtagcttt gctgattttc ggggtgctgg cgattgtttt cggtatgcag
    12421 tttttttcac ttggcttgat tagcgagatg attactttcc agaacaggaa agaaaaataa
    12481 tgcccaaata taggttttcc gtcattattc cgtcatataa tcgagcggac gaagtaattg
    12541 atttactgaa atcgttacaa gcccagactt gcgagcatag tcgtttcgaa gtactggtga
    12601 ttgacgacgg ttcgacggac gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 ncactcgcaa aaacggctgg caaaatttta cccgcgcagt ttcaatatgg gcatccggcg
    12781 tgagatttat gaaaaattag gtggtatggg taacctacgg cacggtcagg atatcgaatt
    12841 cagtcgccga attgtagcca gcggcgctaa ggtcatttat ataccgaacg ccatagttta
    12901 tcataaacgg cgcaccagct tgcgcaaatt ctttcgccag atgttcaact ggggagtggc
    12961 gcgtataaac ctgagttatc tggatgcggg caatttagaa ccgttgcatt ttattccggc
    13021 gatagccgcg gtcggctata ccttattgac gatatttacc ttattgactg gtttagcctg
    13081 gaaaatatgg gttgcaatta ccgttctggc actgctaatc tgtgggtaca ttttcattga
    13141 cagctcgatt aaatataaat ccgtgaaagt cggctggctg gcgcctattt ttgcagtttg
    13201 ccagattttt gcctatggtt atggatttac ttataattat gttcgccgga aaatcttccg
    13261 ccgcggcgaa gttaccgggt tcgtgcggag atattatcag tgacaaatat ttccccccga
    13321 aatctcaatt tccataaaga acaatactgg acggcggcca attttttaga aaaatatata
    13381 aatatttctc ctgtttcagg actcagaatt cttgaagttg gttcggcgga aggtggtggg
    13441 ttaaattatt ttgcgactaa gaataatcaa tgttatggtt tggaatattc gcgcgggcgc
    13501 ttagaaatcg cccgggagtt aaataagtcc tctaagatta tctttattca cggtgatatt
    13561 acggaagcaa gttcctacga gaatcaactt caagataaaa tggacttgat catatgcggt
    13621 gatgtgattg aacatattgc caatgaaaaa aaggcggcgg cactccaaaa tatgcgggat
    13681 ttattgcaaa aaggcggtcg catttatatt tcctttccac cgaagtattc gccttttgcg
    13741 ggacatcagc aggggctacc gggttggcta catcggattc cctatctctt tttattaccg
    13801 gataaatttt attccgcgat tcttaaatcg cagggaattc gcgctgacat tatatcgaat
    13861 atgttggcaa ccaaacattc tcggctttca ataaaacaat ttgaaaaaat ggtttatgaa
    13921 aatggactac gaattctgaa aaaggatttt ttccttatac gtccccgttt taaatataat
    13981 tatggtattc cttacatagg acatcgctgt gatttaccaa ttgttcgaga gatttttaac
    14041 ct
//
LOCUS       DAOSMP010000048        13784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_4471_length_13784_cov_19.778862, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000048 DAOSMP010000000
VERSION     DAOSMP010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 13784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13784
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4471_length_13784_cov_19.778862"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PKX09_08030"
     CDS             complement(<1..601)
                     /locus_tag="PKX09_08030"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HOU17808.1"
                     /translation="MKILKGIRISPGVAIGPAYYVETGKVPIPHTYIDQSEVEAELEK
                     FNRATQRATEELNQVRDMVMKHLDEEHARLIDAQIMTLADVDLVQKMTDMVRQEHRNV
                     IWAYDAAMEWYETALLNTANKFQQERLIDLQDIKRRMFLHLSEQGQFSLPQIPEKTIY
                     VSERISPSELIQLHNQKILGIITRIGGMDSHSGILARAFG"
     gene            complement(605..871)
                     /locus_tag="PKX09_08035"
     CDS             complement(605..871)
                     /locus_tag="PKX09_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOU17809.1"
                     /translation="MVRQTVKILNRHGLHTRPATVLVTLCSRFKSDIFLIYKNVRVNA
                     KSILGVLVLAVEPNAEITIEVNGPDEQEALAAIVELIANKFNTE"
     gene            complement(873..1844)
                     /locus_tag="PKX09_08040"
     CDS             complement(873..1844)
                     /locus_tag="PKX09_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOU17810.1"
                     /translation="MSVDFSTKILQLKEEIDRELSKIDLSGISAELAQPMRFSLNAKA
                     KRLRPILVLIVGENLGASRKNLMPAALAVEIMHTFTLVHDDIMDNDTIRRGQQTVHVK
                     WNVNTAILTGDALMALAFRTLMQTKSEQVNQIGTHFSQAMLEICDGQAFDLAYEKYDE
                     IDVTDYLNMIAHKTGRLLGLACQLATIIANASPKVVNNLYLFGLELGQAFQIQDDLLE
                     LTADQSVMGKSLGSDITAGKKTFPILTALNDLSANQRADLLNFLKINAGNRRIIVQEL
                     TKRGSIQQTEAMITRLFKNARERLHNLPDQLQTDLTNLIELIAGRQS"
     gene            complement(1918..3270)
                     /locus_tag="PKX09_08045"
     CDS             complement(1918..3270)
                     /locus_tag="PKX09_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOU17811.1"
                     /translation="MNPYIFREYDIRGVVADDFTDEVVELLGKGIGTYFREKDAKTIS
                     LSGDIRLSTPHLKSILTKGLLSTGIDIIDLGIVPTPVNYYSMYVLPVDGAIQITGSHN
                     PPDMNGFKISLDKKAVFGEQIQHIKNIIEKGVFAVGAGKVSEKSLLEEYKQMIISKIQ
                     LARPLKIAMDCGNGAASLTAPEIFKKIGCQVTELYCEVDGRFPNHHPDPTVSKNLKDL
                     IAEVQRGDYDFGVAFDGDADRIGVVDDLGQIIWADYLMILFLEEVARPGIPVIFDVKC
                     SQALEEMITAYGAKPIMWKTGHSLIKQKMKDENAPFAGEMSGHLFMGDEYFGYDDAVY
                     VAARLARLIARGNEKISAKTARLPRYFSTPEIRLKCKDDPTKFKISQQATAYFKSHYD
                     CIDVDGVRIKFGDGWGLVRASNTQPVIVTRFEAKTAERLTEIRDLVLNKLREFGELTL
                     "
     gene            complement(3694..>4059)
                     /locus_tag="PKX09_08050"
     CDS             complement(3694..>4059)
                     /locus_tag="PKX09_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HOU17812.1"
                     /translation="LVAIGMFRASGAMDIFVALFSPITNLIGMPAETLPVALMRPLSG
                     SGALGLVTELMKQHGPDSFIGRVASTMWGSTETTFYVVAVYFGSVGVRRVRHSIAAGL
                     VGDAVGLIMAVIICRLVFG"
     assembly_gap    4060..4159
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4160..4677)
                     /locus_tag="PKX09_08055"
     CDS             complement(<4160..4677)
                     /locus_tag="PKX09_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOU17813.1"
                     /translation="MNFIWLGLMLIALIVGALNGRIELVMKAAFDNAAAAVEIALGLI
                     GIMALWLGIMKIAEKGGIIKILSRAIRPIAKFLFPSIPSDHPAIGSMLMNMIANWLGL
                     GNAATPLGLKAMEELQSLNQSKDTATNDMVTFLALNTGTICLIPMTVIAVRAQLGSAN
                     PFEIIGTTIISST"
     gene            complement(4999..5559)
                     /locus_tag="PKX09_08060"
     CDS             complement(4999..5559)
                     /locus_tag="PKX09_08060"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOU17814.1"
                     /translation="MDFEEKCINSKRVFNGRLLQVYRDEVRLPNGKTSNREWIKHPGA
                     AVVIPYLGNGQILLIKQFRYPVRQIMLELPAGKIDPDENPDQTIRRELAEETGYSAQK
                     LTKLGLIHTCVGYSNECLHLFWADELQKCDTRPDDDELIEMIPMSINAAMNLLYQGKI
                     TDAKTIIGLFWADNIINRRYLNLCSD"
     gene            6000..6512
                     /locus_tag="PKX09_08065"
     CDS             6000..6512
                     /locus_tag="PKX09_08065"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOU17815.1"
                     /translation="MKVRINSLKYGKQMIRAALGGDELDVADLDLSAPVAVMLNIDRG
                     TTEIKIDGKVESIARFGCDRCLESFSMPISGTFAIIATYSESNVKIDDENVIQLSSMT
                     NEIDITTQIHDTLLLSIPMKILCQEDCQGLCPICGANHNKTECHCNVAEPDARWDSLK
                     KLTIKITEDQ"
     gene            6514..6696
                     /gene="rpmF"
                     /locus_tag="PKX09_08070"
     CDS             6514..6696
                     /gene="rpmF"
                     /locus_tag="PKX09_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOU17816.1"
                     /translation="MPQPKRRTSKSRRDKRRTHQVLTEATVVKCPQCKEPKLPHRACP
                     NCGYYRGRPVIIPKEK"
     gene            6798..7805
                     /locus_tag="PKX09_08075"
     CDS             6798..7805
                     /locus_tag="PKX09_08075"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOU17817.1"
                     /translation="MKKPKSTITAFGMYVPERILTNADLEKMVDTTDEWIRTRTGIRE
                     RHIAAEGEATSAMAIKAFQDLQRRFNVDPLTIDLIIVATISPDMFFPSTAALIQEGIG
                     AKNAYGFDLSAACSGFIYALANAAQFVENGTCRRVLVFGADTMSSITDYTNRDTCVLF
                     GDAAGVVLLEPTPPGDDSGIIDFILKMDGSGKDYLYMLGGGSLHPATAETVAKKMHYI
                     YQEGKTVFKFAVKSMADISLELLERNGLTGKDIKLFIPHQANKRIIDASADRLKLSDE
                     QVMINIDRYANTTAATIPLALVEGFDQKRIKRGDLVLMAAFGGGFTWGSLLLRWGIPN
                     D"
     gene            7798..8724
                     /gene="fabD"
                     /locus_tag="PKX09_08080"
     CDS             7798..8724
                     /gene="fabD"
                     /locus_tag="PKX09_08080"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008822424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOU17818.1"
                     /translation="MTKVAFIFPGQASQFVGMGRDFYESSALARQYYELAETRLGFPL
                     KKFSFEGPLSELTKTRITQPAIFVLSCIIFFELKARGYRPDMVAGHSLGEYSALVACG
                     ALTFEDGLDLVKIRAEQMQIASEQKPGTMAAIVGLDYDQVKKIISESNFSGICQIANC
                     NSPNQIVISGDVYKVQALMTDLKKAGAKMAVELSVGGAFHSDLMQPASEALKEALSKT
                     EFKTPDCPIYSNVTATATRDPQEIRNRLYQQLTSPVLWTDTIRNMIADGAEIFVEVGP
                     GKVLQGLVKRTSADVNVTGVSKFSELEQIAWN"
     gene            8715..9461
                     /gene="fabG"
                     /locus_tag="PKX09_08085"
     CDS             8715..9461
                     /gene="fabG"
                     /locus_tag="PKX09_08085"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOU17819.1"
                     /translation="MELTFKDKIVIVTGSGRGIGLTIAQTFAENGASVILSDYNQESL
                     ADAVALFKAKNFPFDALPCDVTNSTEVQNLIESIIQKYTRIDVLINNAGVTRDAILLR
                     MKEEQWDAVIDTNLKGVFLVCRAAAKYFLKQQSGKIINISSVVGLTGNLGQSNYAASK
                     AGVIGFSKSLAKEMASRNITVNVIAPGFIDTAMTAALSDEVRTQFLRNIPLNRMGTPQ
                     EVANAALFLASPLADYITGQVLKVDGGMVM"
     gene            9481..9714
                     /gene="acpP"
                     /locus_tag="PKX09_08090"
     CDS             9481..9714
                     /gene="acpP"
                     /locus_tag="PKX09_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012513553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOU17820.1"
                     /translation="MSNFEKVQEIVAEKLGIEKSKITMEASFIDDLGADSLDTVELIM
                     KMEEDFGIEIPDEDAEKLKTVGDVVNYLDSKLK"
     gene            9723..10970
                     /gene="fabF"
                     /locus_tag="PKX09_08095"
     CDS             9723..10970
                     /gene="fabF"
                     /locus_tag="PKX09_08095"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOU17821.1"
                     /translation="MPKRVVVTGMGVISPVGSDVPSFWSNLLNGISGVDYIKSFDASG
                     YSTQIAAEVRDFDPLKYFTQKDINRMDRVTQFGSAAAKQAVYDSGLIESGFSAERVGV
                     IVGSGIGGISTLEAQHQILMTKGHRMVSPLFIPMMIPDIIPGQIAMDFGFCGPNYGIT
                     SACATGSHAIGVAYQHICNGTADVMITGGAEAAITPLAIAGFANMKALSRCNNAPQKA
                     SRPFDSKRDGFVVGEGSGIVVLEELEHALNRKAKIYAEIVGFGFSADAYHITAPHPEG
                     KGAIISMENAIRMSGLPKSAFGYINAHGTSTPLNDRNETVAIKAVFGEQAYQIHISST
                     KSMTGHLLGAAGGIEFITLALALQNQIIPPTINYEFPDPECDLNYTPNIAQKATLVAG
                     LSNNFGFGGHNAVLAAKVYKPEN"
     gene            10967..11704
                     /gene="rnc"
                     /locus_tag="PKX09_08100"
     CDS             10967..11704
                     /gene="rnc"
                     /locus_tag="PKX09_08100"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOU17822.1"
                     /translation="MKVSVKNWFRRISPIEEEIGYVFRNKALLEQALTHRSRSVNGEK
                     SYERLEFLGDAVLAFIVAEILFEKYPDKSEGELTMHRSALVNGNALARVGSKLGIEKA
                     VIVEKGLNLKNPPTLHKLLASIVESLIGAIYLDRGLQPARKFVQRWIIPSTAEGKVVS
                     NFNYKGRLFEYCQKNGRNLPVFKVLRVSGPDHAKKYEVAVYINGQKMGYGVGRQKRNA
                     EQKAAHEALQKLMPEHTVAGNQILKDS"
     gene            11935..>13784
                     /locus_tag="PKX09_08105"
     CDS             11935..>13784
                     /locus_tag="PKX09_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HOU17823.1"
                     /translation="MEVTQKIGLLDWAVVAIYFALIFGIAIYYYRRAGENTGEFFLSG
                     RNLPWWLAGTAMVATTFAADTPLAVTELVAKNGIAGNWLWWNLAIGAMLTVFFFARLW
                     RRAGIMTDVEFTEMRYSGKTAAALRGFRALYLGIFMNTIVMGWVHKAMEKIFHVTIPG
                     IDPFLLVAIVAGIVAIYASASGLLGTARTDSFQFVFAMTGCIILAVIVIRLPEVGGAV
                     KMKAQLAPQVLDFFPKVGRINGEGITGGVLSLSLGAFIAHIGLQWWSSWYPGAEPGGG
                     GYIAQRMMSAKNEKHSLLATLWFTIAHYTLRPWPWIIVALSALVILPRASSTDILQQE
                     NPVLYHQVVEAYKNPDLMKSNPLASTPEFQNFYEKYENTVDPGVMYPKMMLRYLPTGI
                     LGLLIATFLAAYMSTISSQLNWGTSYIINDFYRRFLRKKASEKHYVLVSRIAMLIMIV
                     FSLFVTKYFLTTISGAWEFIINASAGMGAVLILRWFWWRINAWSEIAAMIAPLIIYPI
                     ARYKFGLQSPITLYPVVLGTTIVWLLVTFLTKPEKDSTLTSFYRKVHPGGRGWKKIAE
                     QLPDVKGDSGFGRAFICWIFGVLLVYASLFGIGKIIFHEYLLAGVYFLVAG"
BASE COUNT         3813 a         2916 c         3047 g         3908 t
ORIGIN      
        1 ttccaaatgc tcgggctaga attcccgaat gtgaatccat cccaccgata cgagtgatga
       61 ttcccagtat tttctgatta tgtaattgaa tcagttcaga tggtgaaatg cgttcgctga
      121 catatatcgt tttttcgggt atctgaggaa ggctaaattg tccctgttcc gaaagatgga
      181 ggaacatacg tcttttgata tcttgcagat caatcagacg ctcttgctga aatttatttg
      241 cggtatttaa caaagcagtc tcataccact ccatggcggc atcataagcc cagatcacat
      301 tacgatgctc ctgccgcacc atatcggtca ttttttggac taagtctaca tcagctaatg
      361 tcattatttg agcgtcaatc aaccgggcgt gttcttcatc gaggtgcttc attaccatat
      421 cgcgtacctg gtttaattcc tccgtggcgc gttgagtagc ccgattgaat ttttccagct
      481 cggcttcgac ttcggattga tctatataag tatggggaat tggaactttt ccagtttcaa
      541 cataatacgc cggaccaata gcaacgccgg gagagattcg gatacctttg agaatcttca
      601 ctttttattc cgtattgaat ttattggcta ttaattcaac tatagcggct aaagcctctt
      661 gttcgtcagg tccattaacc tcaatagtga tttcggcatt cggctcgacc gctaaaacca
      721 gtactccaag aatactctta gcattgacac gcacattttt atagattaaa aatatatcgg
      781 atttaaaacg agaacacagg gttaccaaaa cggtagccgg tcgcgtatga agaccatgcc
      841 gatttagaat tttaacggtt tgtcggacca tctcaggatt ggcgccccgc tattaattct
      901 atcaaattag tcaagtcggt ttgtaattga tcgggcaaat tgtgtaatct ttcgcgggcg
      961 tttttaaaaa gtcgggtgat catagcttcc gtctgttgaa ttgaaccgcg tttggtcaat
     1021 tcctgaacga ttattcgccg attaccggca ttgattttga gaaaattaag taaatcagcg
     1081 cgctggttcg ctgataaatc attcagcgca gtaagaatag ggaaagtttt tttccctgcc
     1141 gtaatatcgc ttccgagact tttacccatt acggactgat cagccgtcag ctccagcaaa
     1201 tcgtcctgaa tctgaaaggc ttgtcccaat tccagtccaa aaagataaag attattcacg
     1261 accttcggac tagcattggc aatgattgtg gccagctgac aggccaagcc tagcagtcgg
     1321 ccggttttat gagcaatcat attcaggtaa tcggtgacat caatctcgtc atatttttca
     1381 taggccaggt cgaaagcctg gccatcgcaa atttccagca tagcctggct gaagtgagtt
     1441 ccaatctgat tgacttgctc ggatttggtt tgcattagag tgcgaaaagc cagcgccatc
     1501 aaagcatcgc cggtcaagat agcggtattg acattccatt tcacatgaac agtctgctga
     1561 ccgcgtcgaa tggtgtcatt gtccataata tcgtcatgca ctagcgtaaa ggtgtgcata
     1621 atttcaacag ccagagcggc tggcatcagg tttttccggc tggcgcctaa attttctccg
     1681 actattagta caagaatcgg tcgcagacgt ttggctttgg cattcaggct aaagcgcatc
     1741 ggctgtgcga gttcggccga gattcccgat agatcaattt tacttaattc gcggtctatc
     1801 tcttctttca gttgcagaat cttagtgctg aagtctactg acataaatcc tcattcgtaa
     1861 agtccacttc caattttacg ataattttca aggaaaaaaa attagactat aattttatta
     1921 aagtgtcaat tcgccaaatt cacgcaattt attgagtacc aaatcacgga tttcagtcaa
     1981 gcgttcggcg gttttcgcct caaagcgcgt cacgatcacc ggctgagtat tggaggctcg
     2041 taccaatccc caaccatcgc cgaatttgat tcgcacgccg tcaacatcta tgcagtcgta
     2101 atgagacttg aaataagcgg ttgcttgctg agaaatcttg aatttcgtgg gatcgtcctt
     2161 acatttcaga cgaatttccg gtgtactgaa ataacggggt aagcgtgcag ttttagcgga
     2221 aattttctca ttgccacgcg cgatcaaacg ggcaagtcgc gccgccacat agacagcatc
     2281 gtcatagccg aagtattcgt cacccataaa taaatgcccg ctcatttcac cagcgaaagg
     2341 tgcattttca tccttcattt tctgtttaat aagagaatgg ccagttttcc acataatcgg
     2401 tttggcgccg taagccgtga tcatttcttc cagtgcctgg gaacatttga catcgaaaat
     2461 aaccggtatg cccggtcggg cgacttcttc aaggaacaga atcataagat aatcagccca
     2521 aatgatttgc ccgagatcat caaccactcc gatgcggtcg gcgtcaccgt cgaacgccac
     2581 cccgaaatcg taatcgcctc tctgcacctc agcgatcaag tctttcaaat ttttggaaac
     2641 cgtcggatca ggatgatgat ttgggaatct gccatcaacc tcacagtaga gttctgtaac
     2701 ctgacatcca atttttttaa aaatttccgg agcggtcaaa gaagcggcgc cattaccaca
     2761 atccatagca attttcaaag ggcgagctaa ttggattttg gatatgatca tttgtttgta
     2821 ttcttccagt agtgattttt cgctcacttt gccagcgcca acggcaaaaa cgcctttttc
     2881 gatgatattt ttaatatgtt gaatttgctc cccgaacacg gcctttttat ccagacttat
     2941 cttaaagcca ttcatatcgg gtggattgtg actgccggta atttggatag caccatctac
     3001 cggcaagacg tacatactgt aataattgac cggcgtcggc acgataccca aatcaataat
     3061 atctatgccg gttgataata gccctttggt caaaatggat ttcaagtgcg gcgtcgaaag
     3121 ccggatgtcg ccactgagcg aaattgtttt ggcatctttt tcccgaaagt aagtgccgat
     3181 gcccttaccc agcaattcga caacttcatc agtgaaatcg tcagctacga cgccccgaat
     3241 atcatattcg cggaaaatat acggattcat ttagtcctcc taagaattga tttttccaaa
     3301 aatctctgcg gatgctttag aactttgact attatttttc tggtaatttt aatttcagtc
     3361 gagatcatct ttttatcata agatatataa aagcgaggat ttttaaaaga ctattgaaag
     3421 gaaataatta ctgatttatt tgtgttcact gattccttta cctgatgagt attcttctgt
     3481 gtttctcgag ggataaggct atctaacttt ttagaaaaac cgggcgaatt tggggtaaca
     3541 atccatcgaa cgcaatttta cccactgata gaattaggct gatttacaag aagcataaat
     3601 atttatccgg ttttgtcgag gatttctgct ttggcttgat gctcacaatt taacgctaag
     3661 acttggaaat attttagtga gataattcag tatttatccg aaaaccagac gacaaataat
     3721 aactgccatt atcaatccca ccgcatcgcc gaccaatccc gctgcaatcg agtgacgaac
     3781 ccgccgcact ccaactgaac cgaaataaac cgctactaca tagaaagtcg tttcagtact
     3841 gccccacata gtcgaggcaa cgcgtccgat gaatgaatcc ggtccatgct gtttcattaa
     3901 ttctgtaacc aatcccagcg cgccgcttcc ggacaaaggt cgcatcaaag ccaccggcag
     3961 agtctcagct ggcatgccaa tgaggttcgt tatcggtgaa aacaaagcaa cgaatatatc
     4021 cattgcaccc gaagctcgaa acattccgat tgctaccaan nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnng ttgatgaaat aattgtggtg ccaattattt cgaatggatt
     4201 agctgaaccg agttgagcgc gcaccgcaat aacagtcatt ggaatcagac agattgtacc
     4261 ggtattcagc gccaagaagg ttaccatatc attcgttgcg gtatctttgg attggtttag
     4321 agactgtaat tcttccatag ctttcaagcc cagtggggtg gccgcattgc ctaatcccaa
     4381 ccagttggct atcatattca tcaacattga accgatagcc ggatgatcgg aaggaatgga
     4441 agggaataaa aatttggcta tcggacggat tgcccgcgaa aggattttta ttataccgcc
     4501 tttttcggca attttcataa tgccaagcca gagcgccata attccgatta agcccagggc
     4561 aatttcaacc gccgcggccg cattatcgaa tgccgccttc ataaccagct cgatgcgtcc
     4621 atttaaggcg ccgacaatca gagcaatcaa catcaaacct aaccagataa aattcatatt
     4681 agcctcttca tttttctaat taaaaataaa atgactttgt tgccatcaaa tagtggaaat
     4741 aaacatcatc gttggtcata ggaaatatta tgcaagccgg atggattaag ccattcttga
     4801 attatcctca aggtttgacc atctgctgca aaaccaatca aaaaataaaa acgtctcaag
     4861 gaagtcaaaa ataaagtagc tggattcagc ctcattggaa attaatttat agtcgaaatg
     4921 gctgaaagtc aatgtaggaa agaattacag ttttcccata agtgatttag ttcggggaaa
     4981 aattttatat tttacggttt agtcagaaca tagattaaga tagcggcgat taattatatt
     5041 atcagcccag aataaaccga taatagtttt ggcatcggta atcttgccct ggtaaaggag
     5101 attcatagcc gcattgattg acataggaat catttcaatt aattcgtcat catccgggcg
     5161 tgtgtcacat ttttggagtt cgtctgccca aaataagtgc agacattcat tgctgtagcc
     5221 aacgcaggta tgaattaggc caagtttagt aagtttttgg gctgaatatc cggtttcttc
     5281 ggctaattca cgccggatgg tctgatccgg gttttcgtcc ggatcaattt ttccagctgg
     5341 tagttccagc attatttgcc tgacgggata gcgaaattgc tttatcagaa gaatctggcc
     5401 gtttcccaga taaggaatga ctactgcggc tcccggatgc ttgatccatt cgcggttgga
     5461 agttttacca ttaggcaggc gaacttcatc gcgataaacc tgtaataagc gcccattaaa
     5521 aacacgttta ctattgatac atttttcttc aaaatccata tttttatacc caaaaatcct
     5581 ttttataatt ttatcaaaaa atgacgattt ttgataaaaa tacgtcagcc tttttaaagt
     5641 atttctatga aaatttagcc gtgaagataa aaggagagga ggcgttctat tattaacggg
     5701 aggagttccc aaggaaggag ggaagttgga tgaaactcat cctgaaatgg gagtgatgtc
     5761 ccactcagtg aacgcctcct ctttaatcct ttttcaaata tcgtaaactt taacaattat
     5821 ttttcaatta agttccggct aaatttattt aatcacctgg aacaaagcaa ggattatcaa
     5881 tgccggaaaa ttttattgaa atatggggaa ataatcaaag ttcaataggt ggtttctcct
     5941 cggaaagagg ttaaaattct tgattatcgt gttcgtttcc aataaatttg caagttatta
     6001 tgaaagttcg tattaattcg ctcaaatatg gaaagcagat gatcagagca gctctcgggg
     6061 gcgatgagct ggatgtcgct gaccttgatc taagcgcccc ggtggcggta atgctgaaca
     6121 ttgacagggg caccacagaa ataaaaatag atggtaaagt tgaatccatc gcccgatttg
     6181 gctgcgatcg ttgtttggaa agtttttcga tgccaataag tggaactttc gcaatcattg
     6241 ctacctattc cgaatctaat gtgaaaatag acgatgaaaa tgttatccaa ctttcatcaa
     6301 tgacaaatga aattgacata actacccaaa ttcacgacac tcttttattg agtataccga
     6361 tgaaaatcct ctgccaagaa gattgtcagg gactttgccc gatatgcggt gctaatcata
     6421 acaagactga atgtcattgc aatgtcgctg agcctgatgc ccgctgggat tcattaaaaa
     6481 aactaaccat aaaaattacg gaggaccaat aaattgccac aaccgaaacg tagaacatca
     6541 aaatcaagaa gagataaacg ccggactcat caagtcctga ccgaagccac ggtcgttaag
     6601 tgccctcaat gtaaagaacc taaattaccg caccgagcct gccccaattg tggctattac
     6661 cgtgggcggc cggtaattat acctaaagaa aaatagggct ttagctggtt gaaattttca
     6721 caagcagtcc gatggtatgc catatcctga aatttaactg ggtcagagac aagaatagag
     6781 gttaaggaaa atttctaatg aaaaaaccta aatctactat tactgcgttt gggatgtatg
     6841 tacccgaacg tattttaacc aacgctgacc tggagaaaat ggttgacacg accgatgaat
     6901 ggatccgtac tcggaccggg attcgcgaac gtcacattgc tgccgaaggt gaggcgactt
     6961 cggctatggc tatcaaagcc tttcaggatc ttcaacgacg ctttaatgtc gatcctctga
     7021 cgattgattt gattattgtg gctaccattt ctcccgatat gttctttcca tcaactgccg
     7081 ccttgattca agaaggcatc ggggctaaaa atgcttatgg attcgatctc tcggcggctt
     7141 gttcgggatt catttatgct ctagctaatg ccgcccaatt cgtggaaaat ggtacttgcc
     7201 gccgagtatt ggtgtttggt gctgatacaa tgagtagcat taccgattat accaatcgcg
     7261 atacctgtgt gcttttcgga gacgcggcgg gtgtggtttt gcttgagcca actcctcccg
     7321 gggacgactc aggcattatt gattttatct taaaaatgga tggatcgggc aaagattatt
     7381 tgtatatgct gggtggtggt agtttgcatc cagccacagc cgaaaccgta gctaagaaaa
     7441 tgcactatat ttaccaagaa ggtaaaactg tattcaaatt cgcagtcaag agtatggctg
     7501 acatctctct tgagttactg gaacgtaatg gtttgactgg taaagacatc aaattattca
     7561 ttccccatca agccaataaa cgaattatag atgcttcggc tgatcgttta aaattatcag
     7621 atgaacaggt aatgatcaat attgatcgtt atgccaatac cacggcggca actatcccac
     7681 tggcgctggt tgagggattc gatcagaaac ggatcaagcg cggagacttg gttttgatgg
     7741 cggctttcgg tgggggtttt acctggggca gtcttctttt acgctggggc attccgaatg
     7801 actaaagttg cctttatatt tcccggtcag gcttcgcaat ttgtcgggat gggtcgcgat
     7861 ttttatgaaa gcagtgctct ggcgcgccag tattatgaac tagctgaaac tcggctcggg
     7921 tttccgttaa aaaaattttc cttcgaaggc ccgctgagcg aattgaccaa aacccgcatt
     7981 acccaaccgg cgattttcgt gttatcttgt atcatatttt tcgaattgaa agcgcgcggc
     8041 taccgccctg atatggttgc cggacatagt ctcggcgaat actcagccct ggttgcctgc
     8101 ggcgctctga ccttcgagga tgggcttgat ctggtaaaaa tacgggctga acagatgcag
     8161 attgcctcgg agcaaaaacc cggaacaatg gccgctattg ttggtctgga ttatgaccag
     8221 gttaagaaga taatcagtga atctaatttt tctggcattt gtcagattgc taattgtaat
     8281 tctcctaacc aaatagtaat cagcggcgat gtttacaaag tacaggcttt aatgactgat
     8341 ttaaaaaaag ccggtgctaa aatggctgtg gagttatcag tcggcggagc ctttcattcc
     8401 gatttaatgc aaccagccag tgaagccctg aaggaagctc tgtctaaaac cgagttcaaa
     8461 acgccggatt gtccaattta ttctaatgtc accgctacgg ctacgcgcga tccgcaggaa
     8521 attcgtaacc gactctacca gcaattgact tccccagtgc tctggactga cacgatcagg
     8581 aatatgattg ctgacggagc ggaaatcttc gtggaggttg gacccgggaa agttctacag
     8641 ggattagtca aacggacctc ggctgatgtt aatgtaacag gcgtttctaa attcagcgaa
     8701 ttggagcaga tcgcatggaa ttaactttca aagataaaat agtgattgtc actggttcgg
     8761 ggagagggat aggtttgacc atcgctcaga cttttgccga gaatggcgcc agcgtgattt
     8821 taagcgatta caatcaggaa tcattagctg acgccgtcgc cctttttaag gcaaaaaatt
     8881 ttccgtttga tgcccttcct tgtgatgtaa ccaactctac tgaagtacaa aatttaattg
     8941 aaagcattat tcaaaaatat actcgcattg acgttttaat caataacgct ggggtaaccc
     9001 gcgacgccat ccttttgcga atgaaggaag agcaatggga cgcagttatc gataccaatt
     9061 tgaaaggcgt ttttctggtt tgccgagcgg ccgcaaagta ttttctgaaa cagcaatcgg
     9121 gcaaaatcat caacattagc tcggtagtgg gcttgactgg caacctcggt caatctaatt
     9181 atgcggccag taaagccggt gtgatcggct tttccaaatc gctggctaaa gaaatggctt
     9241 ctcggaatat aacggtcaat gtcatcgcgc ctggattcat tgacacggct atgacagccg
     9301 ctttgtcgga tgaagttcga acgcaatttt taaggaatat tcctttgaat agaatgggga
     9361 cgccgcaaga agtggccaat gcggcgctat ttttagcttc gccgctggct gattatatca
     9421 ccggacaggt gcttaaagtt gacggcggta tggtaatgta aactaactta ggaggatatg
     9481 atgagtaact tcgaaaaagt tcaagaaatc gtggctgaaa aattagggat cgaaaaatcc
     9541 aaaattacga tggaagcttc tttcattgac gatcttggag ccgattcact ggacacggtg
     9601 gagctgatta tgaagatgga agaagatttc ggcattgaaa ttcctgacga agatgctgaa
     9661 aagctaaaaa cagtcggtga tgttgtcaat tatctcgact ccaaattaaa ataaccccgt
     9721 ctatgcccaa acgtgtagtt gtgaccggaa tgggcgtcat ttccccggtt ggaagtgacg
     9781 tcccatcttt ctggtcgaat ttgctaaatg gtatcagcgg cgtcgactat attaagtctt
     9841 ttgatgccag cggctattcc actcagattg ccgccgaggt gcgcgatttt gatcctctga
     9901 agtacttcac tcaaaaggat atcaatcgaa tggatcgcgt tactcaattc ggatcggcgg
     9961 ccgccaaaca ggctgtatat gactcaggat taattgaatc gggtttctca gccgaacgag
    10021 taggagtaat cgtcggatcg ggcattggtg gaatcagtac cctggaagcc cagcatcaaa
    10081 ttttgatgac caaaggtcat cgcatggtaa gtccactctt tatcccgatg atgattccgg
    10141 atataattcc cggccaaatt gcgatggact ttggtttctg cggtccaaac tatggcataa
    10201 cttccgcttg tgctactggt tcacatgcca ttggagtcgc ctatcaacac atctgtaatg
    10261 gaactgccga cgtaatgata accggtggtg cagaagccgc tataacaccg ctggcaatcg
    10321 ctggtttcgc caatatgaaa gccctctcac gatgtaataa tgcgccacaa aaagccagcc
    10381 ggccattcga tagcaaacgt gatggcttcg tagtaggtga aggatccggc atcgtagtcc
    10441 tcgaagaatt ggaacatgcc ctgaaccgga aggctaaaat ttatgccgaa atcgtcggct
    10501 tcggtttctc agccgatgcc tatcatatca ctgctcctca tccggaaggc aagggagcga
    10561 ttatttccat ggaaaacgcc atccgaatgt caggcttgcc gaaatcagca ttcggctaca
    10621 tcaatgccca cggcacctcc accccgttaa atgatcgtaa cgaaacggtc gccattaaag
    10681 ctgttttcgg cgagcaggct taccagattc atatcagttc taccaaatca atgaccgggc
    10741 atcttttagg tgcggctggc ggaatcgaat tcatcactct ggcgcttgca ctccagaacc
    10801 agattatacc gcccactatt aattatgaat ttcccgaccc ggagtgcgat ttaaactata
    10861 cgcccaacat tgctcagaaa gcaaccctag tagctggatt aagtaacaat tttggttttg
    10921 gggggcacaa tgccgtcctg gccgccaaag tttacaagcc ggaaaattga aagtatcggt
    10981 aaaaaattgg tttcgcagga tttcgccaat tgaggaggaa ataggatacg ttttccgtaa
    11041 taaagctcta ttggaacagg ctcttactca ccgctcgcgt tcggtgaatg gcgaaaaatc
    11101 ctatgagcga ttagaatttc tcggcgacgc cgttctggct tttatcgtcg ccgagatttt
    11161 atttgaaaaa tatccggata aaagtgaggg cgaactgacg atgcatcgct cggctctcgt
    11221 aaatggaaat gccctggcac gcgttggtag taaattggga atcgaaaaag cagtcatcgt
    11281 cgaaaagggt ttaaatctca aaaatccgcc gactctgcat aaattacttg ccagtattgt
    11341 cgaatcttta atcggtgcaa tctatctcga ccgtggactg cagccggctc gcaagttcgt
    11401 tcaacgctgg attataccat caacagccga aggcaaagtc gtcagtaatt ttaattacaa
    11461 gggacggctt ttcgaatatt gccagaaaaa tggacgcaac ttgccggttt ttaaagtact
    11521 aagagtctct ggaccggatc atgccaagaa atatgaagtt gcggtctata tcaatggtca
    11581 aaaaatggga tatggcgttg gtcgtcagaa acgcaatgcc gaacagaaag ccgctcatga
    11641 agctctgcaa aaattgatgc ctgaacacac tgttgccgga aatcagattt taaaagattc
    11701 ataatattat tttgaaaatc cggttgaatt tcgcgattga catttaaata tttggtaatt
    11761 attcagaaat ggtacagtaa gcctagtcaa tatttagacc taaacatttt tgatttaagc
    11821 aaattcgatt tgtaccaatg atgtttcctt cgattgataa gccttcaaag aatttagtaa
    11881 tatcgaatta actacttaat tattagttat taataaggtt aatgagaggt aaaaatggaa
    11941 gtgacacaga aaatcggatt gttagattgg gccgtagtgg caatatattt tgcgctaatt
    12001 tttggcattg caatttatta ttaccggcgg gctggggaaa acactggtga attttttctc
    12061 tccggaagga atttaccttg gtggttagcc ggaactgcca tggtagcgac aacctttgcg
    12121 gctgatacac ctttagctgt tacggagctc gtcgctaaga atggtatcgc cggtaactgg
    12181 ctatggtgga atctggctat cggtgcgatg ctgacggtct ttttctttgc tcgactatgg
    12241 cgccgagcag gaattatgac tgatgtagaa tttactgaga tgcggtattc cggtaaaacg
    12301 gcggcggctc tgcgcggatt ccgagcccta tatctgggta tttttatgaa tacaattgtt
    12361 atgggatggg tacataaagc catggagaaa atattccatg tcacgatccc agggattgac
    12421 ccgtttcttt tagtagcaat tgtcgctggt attgtcgcaa tttacgcttc agcttccggc
    12481 ctgcttggga cggctcgtac cgacagtttc cagtttgtat ttgctatgac tggttgcatt
    12541 atattggctg ttatagtcat ccgactgcca gaggttggtg gagccgttaa aatgaaagct
    12601 caattggcac cgcaggttct tgatttcttt cccaaagtgg ggcggattaa cggagaggga
    12661 attaccggtg gggtactatc tctatcattg ggtgccttta ttgcccatat cggactgcaa
    12721 tggtggtcgt cctggtatcc aggggctgaa ccaggcggcg gcggttacat tgcgcaacgc
    12781 atgatgtccg ctaagaatga aaaacacagc cttttggcaa ctctctggtt cacaatagcg
    12841 cattatactt tacgtccatg gccttggatt attgtagctc tttcggcatt ggtgattcta
    12901 ccgcgagcct caagtaccga tatacttcag caagaaaatc cggtacttta ccatcaggtc
    12961 gttgaagcgt ataaaaatcc tgatcttatg aaatctaatc cgcttgcttc taccccggaa
    13021 tttcaaaatt tttacgaaaa atatgaaaat accgtcgatc ccggagttat gtatcctaag
    13081 atgatgttac gttacttacc tacaggaatt ttaggtctat tgattgcgac ttttttagcc
    13141 gcttatatgt caactatctc ttcccaatta aactggggca catcatatat tataaatgat
    13201 ttttaccgaa gattcctgcg taaaaaggct agtgaaaaac attatgtatt ggtctcccga
    13261 attgcaatgc tgattatgat tgtattttca ttattcgtca cgaaatattt cctgactact
    13321 atatcaggag catgggaatt tatcatcaac gctagtgcgg ggatgggtgc agttctcatt
    13381 ttacgctggt tttggtggcg tatcaatgcc tggtcggaaa ttgcggctat gattgcacca
    13441 ctaattatct atccaattgc acggtataaa ttcggactgc agtccccgat aacgctttat
    13501 ccggtagtcc taggtactac gattgtttgg ctactggtga cttttttaac caaaccggaa
    13561 aaagactcta ctcttaccag cttttatcgt aaggtgcatc ctggtggtcg cggttggaag
    13621 aaaattgctg aacaattacc tgatgtcaag ggcgatagtg gtttcggccg agcatttatc
    13681 tgctggattt ttggggtttt gctggtctat gccagcctgt ttggaatagg aaaaatcatt
    13741 tttcacgaat atcttttagc aggagtgtat tttttagtgg ctgg
//
LOCUS       DAOSMP010000049        13398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_4634_length_13398_cov_20.335682, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000049 DAOSMP010000000
VERSION     DAOSMP010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4634_length_13398_cov_20.335682"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            337..747
                     /locus_tag="PKX09_08110"
     CDS             337..747
                     /locus_tag="PKX09_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17824.1"
                     /translation="MTYDSYVMAEYNWWNRYPPTYPNYYYAGDFYTFMGGTIDYIPAL
                     TYDPIGGGLAKTVAYDLNEPGSNPPTGSASSNFLDSELIEALDYLFDEKYEDAIAIYV
                     RRLNNETDKSKQKYILSCIAECYELSGKDGFIEQ"
     gene            737..1393
                     /locus_tag="PKX09_08115"
     CDS             737..1393
                     /locus_tag="PKX09_08115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU17825.1"
                     /translation="MNNDVRKNLSRKDELYAKILELENYVLMRAGKFQEAIDNYLIMK
                     NEFADNEAIQKNALFNLGYLHYVALDNRVQGQAYFEEFIEKYPDDDLNLIVYETMGES
                     EKLKLIKKSAPPIADEADIAVLPSEYRLLNNYPNPFNPLTTIAYDLPQESQVMLTIYD
                     ILGREVIRLVDERQPAGIQKVIWDGRDSYGQLVPSGIYLYSLKTTSGFNATEKMIFMR
                     "
     gene            complement(1485..2258)
                     /gene="kduD"
                     /locus_tag="PKX09_08120"
     CDS             complement(1485..2258)
                     /gene="kduD"
                     /locus_tag="PKX09_08120"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HOU17826.1"
                     /translation="MENKNVLDLFKLDGKVALVTGAARGLGQAIATALASAGADVSLV
                     DILPTEETHETIAGYGRRALSLTANLMEKANIEPVVDATVKKFGRIDILVNNAGIIRR
                     APLTEFSEKDWYDVINLNQNTVFFLSQIVAKLMIARGEGGKIINIASLLSFQGGILVP
                     SYTASKSAAMGLTRAMANELAPHHINVNAIAPGYMATDNTAPLRANPARAESILERIP
                     IGRWGEPADLQGVAVFLASSASDYMTGYTVAVDGGWLAR"
     gene            complement(2285..3166)
                     /locus_tag="PKX09_08125"
     CDS             complement(2285..3166)
                     /locus_tag="PKX09_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOU17827.1"
                     /translation="MTLADSLKFEKKHDFLVAIDSDGCAFDTMEIKHKECFIPNIIYY
                     WNLQAVSKYARAAAEFVNLYSKWRGINRFPALTMTFDLLIDWPEVQRRGVTIPRAQSL
                     RDWIARETKLGNPALQAEVERSGDAVLKQALAWSEAVNADIARMVHDVPPFPYVRESL
                     QRLAEVADVMVCSATPTEALRREWEEHDIARYVRAIAGQEVGSKKEQIEQAASARYEP
                     QRILMIGDALGDLKAARANSARFFPINPGHEEDSWENFFKKYLDDFINGQYSEEKEGS
                     LISEFESFLPASPPWKK"
     gene            complement(3193..4644)
                     /gene="gnd"
                     /locus_tag="PKX09_08130"
     CDS             complement(3193..4644)
                     /gene="gnd"
                     /locus_tag="PKX09_08130"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOU17828.1"
                     /translation="MNKADIGLIGLAVMGENLVLNMERHGFTVAVFNRTVEKVDKFLA
                     GRGKGKNIIGTHSIPEFIASLKKPRKIMLMVKAGQPVDDFIELLIPHLEKGDIIIDGG
                     NSHFPDTIRRTKYLEEQGFLYIGTGVSGGEEGALNGPSIMPGGSPEAWPHVKPIFQAI
                     AAKVEDGTPCCDWVGENGAGHFVKMVHNGIEYGDMQLICEAYQIMKDLLGLSYPEMHQ
                     VFADWNEGDLKSYLIEITRDILAYKDEDGQPLVEKILDTAGQKGTGKWTVVASLDAGA
                     PLTMIGEAVYARMLSAMKDERVAASKVLKGPQPEFKGDKQQFIADLGQALYAAKIVSY
                     AQGYVLMRMAAKDFGWHLNYGGIALMWRGGCIIRSVFLGKIKEAFDQNPSLSNLLLDP
                     FFKEKIESSQNAWRRVIATAVTNGIWVPALASALNYFDGFRNSRLPANLLQAQRDYFG
                     AHTYERIDKPRGEFFHTNWTGRGGETSSTTYTV"
     gene            complement(4671..5945)
                     /locus_tag="PKX09_08135"
     CDS             complement(4671..5945)
                     /locus_tag="PKX09_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOU17829.1"
                     /translation="MLYFAKCSADYGLTEAEIRDGFFSALAKLGARRKVIAVPPDFTR
                     FHSQAGQLTRLAFQYYGKNLQAILPALGTHFPMTEAEIEAMFGDLPRHLFRIHHWKED
                     LFTLGVVPGEFVFEVSEGKLDYEWPAQVNKLLVEGGFDLILSIGQVVPHEVIGMANYN
                     KNIFVGTGGHESINKSHFLGAVYGIERIMGRADTPVRRVLNYASDNFARQMPIVYVLT
                     VIGKNSTGQMAIKGLFIGDDYECFQLASELSLTINFDLLDKPLKKVVVYLDPQEFKST
                     WLGNKSIYRTRMAIADGGELIVLAPGLREFGEDPEIDRLIRKYGYRTTPEVMQFVKEN
                     SDLQNNLSAAAHLIHGSSENRFTVIYCPGYISREEIESVNFRYGELAPMLARYDPAKM
                     QDGFNILPDGEEVFYISNPALGLWATKERFNN"
     gene            complement(5967..7049)
                     /locus_tag="PKX09_08140"
     CDS             complement(5967..7049)
                     /locus_tag="PKX09_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOU17830.1"
                     /translation="MNYGLNIKPNGSLDLLSLGALVHRLDPGTVPFRKATECKIHVSG
                     GEYNVAANLADCFRLNTGIATAMADYPIGDLIAERVRAMGVKPFYKHFAHNGVNGPNM
                     ATVYSDRGYGVRAPVVFYNRSNEAAALLKPGDFDWKAIFAEGVRWFHSGGIFAALSET
                     TGQVIIEAMKAAKAAGAVTSFDLNFRAKLWNLWGGEKKAVEVINEIVKYVDVLVGNEE
                     DLQKGLGIPGPEITAKSKLDPSAFIAMISDVVKKYPQIKVVATTLREVHSTNRHSWSA
                     VAWINGATYQAPTAELDVYDRVGGGDGFASGFFYGLLKNVPPEEAVKLGWAHGALVTT
                     YPGDTTMATLAEVENFIKGGSARIQR"
     gene            complement(7084..7896)
                     /locus_tag="PKX09_08145"
     CDS             complement(7084..7896)
                     /locus_tag="PKX09_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU17831.1"
                     /translation="MKTNLNDKTVVITGGSGILGRVMVSELFKAGANVAVLDLREPPT
                     TSIPTSPGKSGNRILFTPTDVLKKESLQNAAAKVTAEFGQVDVLINGAGGNRPDATTS
                     PECSFFDLPAEALRFTMDLNLLGTILTSQVFGKIMAKQKFGNIINITSMNAFRPLTRI
                     PAYSAAKAAVSNFTQWLAVHMAQEYSPNIRVNAIAPGFFLTDQNRFLLTDKETGELTE
                     RGRKIIAATPMGRFGEPEDLIGTLIYLISNSSRFVTGVIIPVDGGFNAYSGV"
     gene            complement(7917..9218)
                     /locus_tag="PKX09_08150"
     CDS             complement(7917..9218)
                     /locus_tag="PKX09_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOU17832.1"
                     /translation="MEWTEVIVLVSSFVFLLLAGVPISFSIGIATLSTMLLSIDTLPA
                     LTTVAQRMATGIDSFALLAIPFFILSGLLMSRGGIARRLIDFAKVLVGRWPGGLAYVN
                     ILAAMFFGAISGSAAAAAAAIGGFMTPLMEKEGYDTNYSASVNITSATTGLLIPPSNI
                     LIVYSLASGGVSIAALFIAGYLPGILVGLLLMVAAGIIAVKKGYRGSDERYTFKQGIM
                     RFLDALPSLMLIVIVIGGIIAGFFTATEGSAIAVLYTFILSVLIYHEVKWKELPQVLL
                     ETVTTTAIVMLLIGTSMAMSWIMSYANIPQTITASLIALSSSKVVILLIINLILLFVG
                     TFMDMTPAVLIFTPIFLPIVTALGIHPIHFGIIMVLNLCIGLCTPPVGSVLFIGCGIA
                     NTTIPKIVKSILPFYAAMILALLLVTYFPQISLFLPRLFGY"
     gene            complement(9205..9738)
                     /locus_tag="PKX09_08155"
     CDS             complement(9205..9738)
                     /locus_tag="PKX09_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOU17833.1"
                     /translation="MLKLRKILDNILKWVVVVLMAISVLNVLWQVFTRFILHHPSSYT
                     EELARYLLIWVGLLGAAYASGHKLHLAIDLFSAKMKKNNAARLQIFIQICVLFFAISV
                     MVIGGLRLVTITLTLNQLSAALKVKLGYVYMVLPISGILIAIYSIVEIIGLFGQLFRP
                     DSTNNLPEKRTEYHGVD"
     gene            complement(9738..10733)
                     /locus_tag="PKX09_08160"
     CDS             complement(9738..10733)
                     /locus_tag="PKX09_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOU17834.1"
                     /translation="MRTKFLTKIGLGTAIIISGFCLNGCESEARTKTIKLAHALDTSH
                     PVHKSLVYMSERFAEKSGGKYRIDIYPSGQLGSEREYVELLQIGSLGMTKVSCSVLEG
                     FVDKMKVFSMPYLFRDDEHRFKVLEGEIGRELLLEGEKYWIRGLCYLDAGCRSFYSVK
                     KPINTPEDLRGLKVRTQESTTSIQMIQAMGGSATPIAWGELYTALQQGIVDAAENNPP
                     SFYISHHHEVAKYFALDEHTAVPDILLISTKVWNSLPPQAQEWLQSAADEAAIYQREL
                     WKIATEEALEEVQKAGVVVSYPDKRPFQEKVKDVYESYRNEPEIYDLIQRIRAVQ"
     gene            complement(10751..12154)
                     /gene="uxaC"
                     /locus_tag="PKX09_08165"
     CDS             complement(10751..12154)
                     /gene="uxaC"
                     /locus_tag="PKX09_08165"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOU17835.1"
                     /translation="MILPSDRFFDSDPAIRSLARELYQQVKDLPIISPHGHIEAKILA
                     ENQPFPDPTALFILPDHYIYRLLYSQGIPLESLGIPTRDGTPVETDHRKIWQIFADNY
                     YLFAGTPTSLWLAYEFSEVFGVTEKLNSANAMAIYDHLQNQLQKPEFRPRALYERFNI
                     EVLTSTDGATDSLKYHRQIKESGWKGRIVPCFRPDALFNINAVGWHREIEKLGDVVGY
                     EINTYVRFIDAIENRREFFKIMGAVSTDQGVESAYTHRLSASQAEEIFQRARNGKASA
                     EDARLFTANMLMEMARMSVADGLVMQMHVGALRNHNLRNFRRFGPDTGADIPVRSEFT
                     VNLRELLNEYGTDSRLTLVVFTLDESTYSRELAPLAGYYPAMKLGAAWWFHDSIQGMI
                     RFREMITETAGFYNTVGFTDDTRSFPSIPARHDLCRRVDSNFLAGLVVRHIIDLDEAH
                     VLIRALTYDLAKKTYKL"
     gene            complement(12151..13206)
                     /locus_tag="PKX09_08170"
     CDS             complement(12151..13206)
                     /locus_tag="PKX09_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU17836.1"
                     /translation="MNKPPNRSPNVTLDDIARQLKVSKVTVSKALRDHPDIGSATKRE
                     VQALAAKLGYVPNFMARNLSARSSRTIGLVVPKLAHYFFATCIESIYNAANASQYDII
                     MTVSQENPEYELKHIQTLLAMRVEGLLVSVTENTTDTSIFQDIKKRGVPIVFFDRIPF
                     GLESNYVVTDDRAAAARLTNYVIQAGYRKIAHFAGYSHTNIGYERQTGFRQALSDNNL
                     PIRKDWIIEGGFGERAGYEALKKVAETKDLPELIFAVTFPVALGIFEAASELGLKIPG
                     DIDLVCFGGGQFNRYLSPALTFMDQPASTIGEKAFNLLLDELHSSERRSEQHIVIPSQ
                     LVIGETCRKLQYNYEEL"
BASE COUNT         3719 a         3282 c         2861 g         3536 t
ORIGIN      
        1 tgcgatcgaa gtttgattta agattgtgca ttagtttatc tccttgttta ttatttattt
       61 ataatacaat aatttaagga tttttaagct aatgctcatc tttttttagg tttttatttc
      121 acacaacggg ttatcagtaa taatggtcag gctggggtat attgtattat caatagtcca
      181 gctctttttg gtgaccgtcg ttgtcatccc ggatataatc tgataactca aaattatacc
      241 ggggtaacct gcggtttaga aagtgatgta agaatcggtg ccccagatct cgcaggttat
      301 aacagcattc atgataacac tggttatgaa gtttcagtga cttatgacag ttatgtcatg
      361 gccgagtata actggtggaa ccgttatccg ccgacctatc cgaattatta ttatgccggt
      421 gatttttaca cctttatggg cggtacgatt gattacattc cggcactgac atatgatccg
      481 atagggggcg gattagccaa aaccgtagca tatgatttaa atgagcccgg ctcaaatcca
      541 ccaacaggct cagccagcag taactttttg gattccgaat taattgaagc cctggattac
      601 ctcttcgatg aaaaatatga agatgccatc gctatatacg ttagaaggtt aaataatgag
      661 actgataaga gcaaacagaa gtatattctc tcctgcattg ccgagtgtta tgagttgtca
      721 ggcaaagatg gttttattga acaatgatgt tcgcaaaaac ctatccagga aggatgaact
      781 ctatgccaag atcctagaac tggagaatta tgtattgatg cgagcgggaa aatttcagga
      841 agccattgac aattatctca tcatgaaaaa tgagtttgct gataatgaag ctatccagaa
      901 aaatgctttg ttcaatcttg gctacctgca ttatgtagcg cttgataatc gcgttcaagg
      961 tcaagcctat ttcgaagagt ttatcgagaa atatcccgac gatgatttga atctaatagt
     1021 atatgagact atgggtgaat cggaaaagtt gaaattaatc aagaaaagcg ctcctccgat
     1081 tgctgatgaa gccgatattg cagttttacc ttcagagtac cgcttgctga ataattaccc
     1141 caatcccttt aatcctttga ctacgattgc ttatgacttg ccgcaagaat cacaagtgat
     1201 gttgacaatt tatgatattc tgggacgtga agtaataaga ttggttgatg agagacagcc
     1261 agctggaata caaaaagtca tctgggatgg cagagatagc tatggtcaat tagtgcccag
     1321 tgggatatat ctctattccc tgaaaacaac ttctggattc aatgcgactg aaaagatgat
     1381 ctttatgcga taatagagaa attatctgac acaacatcaa ccccgtgaga gtaatcagtt
     1441 ttaaaatgcc tcaacgctgt tagtgtgcac taatatattc aaacttatct tgccagccag
     1501 ccgccgtcaa ctgccacggt atagccggtc atataatcgg atgctgacga ggctaagaaa
     1561 accgccacgc cttgtaagtc agccggttcg ccccagcgcc cgataggaat acgctcgagg
     1621 attgattctg ctctcgccgg attagcacgc agaggcgcgg tgttatcggt cgccatatag
     1681 ccgggtgcta tagcattgac attgatatga tgcggcgcga gttcattagc cattgcgcgg
     1741 gtcaatccca tagcggcgct tttcgaagcg gtataggacg gcaccaaaat tccaccctga
     1801 aaggacaata gtgaagcgat attgataatc ttaccgcctt ctcctcgcgc aatcatcaat
     1861 ttagccacaa tctgactgag gaaaaagacc gtattctgat taaggttaat gacatcgtac
     1921 cagtcctttt cggaaaattc cgtcagcggt gcgcgtcgaa taattccggc attattgacc
     1981 agaatatcaa tgcggccgaa ttttttcact gtggcgtcaa ctaccggctc aatattggct
     2041 ttttccatca aattagcggt aaggctgagc gctcggcgac catagccagc aatagtttcg
     2101 tgagtttctt cagttggtaa aatgtccacc aaactgacgt cggctccagc tgaagccaga
     2161 gccgtggcaa tcgcctgccc aagtccccgc gcggcgccgg tgaccagcgc gactttaccg
     2221 tccagtttga ataaatctaa aacatttttg ttttccatat actcctcttt aattgtcttg
     2281 aaatttattt cttccacggc ggagacgccg gtagaaacga ctcaaattca ctaatcagag
     2341 aaccttcctt ttcttctgaa tattgtccgt tgatgaaatc atccaggtat tttttaaaaa
     2401 aattctccca ggaatcttcc tcgtgtcccg gatttattgg gaaaaaacga gctgaattag
     2461 cgcgggcggc tttgagatcg ccgagagcgt cgccaatcat tagaattctc tggggttcat
     2521 atcttgcgct tgccgcctgc tcgatctgct cctttttact gccaacttcc tgcccggcaa
     2581 ttgcccgaac atagcgcgca atgtcatgtt cctcccattc gcgccgcaga gcttccgtgg
     2641 gtgtcgctga gcaaaccatt acatcagcta cttcggcaag acgctgtaat gactctctta
     2701 cataaggaaa cggcggtaca tcgtgaacca ttcgagcgat atcggcattg acggcttcac
     2761 tccaagccag agcttgtttc aaaaccgcat caccgctccg ttcgacttcg gcttggagag
     2821 ccggattgcc gagtttggtt tcgcgggcaa tccaatctcg caaggactga gctctgggaa
     2881 tggttacgcc ccgccgttgc acttccggcc aatcaattaa taagtcaaaa gtcatcgtca
     2941 aagccggaaa gcggttgatc ccccgccatt tggaatagag attgacgaat tcagctgcgg
     3001 cgcgggcata ttttgaaacg gcttgtaaat tccaataata gataatattg ggaataaagc
     3061 attccttgtg tttgatttcc atcgtatcga aagcacagcc gtccgaatct atcgcgacca
     3121 gaaagtcgtg ctttttctca aattttagcg aatctgccag cgtcatcgtt ctgtctcctt
     3181 cgtggaaatg aatcagaccg tataagtcgt ggaagatgtc tctccaccac gtccggtcca
     3241 gttagtatga aagaattctc cacgcggctt gtcaatccgc tcgtaagtat gtgcgccgaa
     3301 ataatcacgc tgagcttgca gaagattagc cggcaggcga ctgttacgga atccatcgaa
     3361 gtaattcaaa gcggaagcca gcgctggaac ccagatgcca ttagtaacgg cggtagcaat
     3421 tacccgacgc catgcgtttt gcgacgattc gattttttcc ttgaaaaatg ggtcgaggag
     3481 taggttactc aggctgggat tttgatcgaa tgcctctttg atttttccca gaaatacgga
     3541 gcgaataata cagccgccgc gccacattaa ggcgatgccg ccgtaattca aatgccagcc
     3601 gaagtcctta gccgccatac gcatcagaac gtagccttgg gcataggaaa cgattttagc
     3661 cgcatacaag gcttgaccga ggtcggcgat gaattgttgc ttgtctcctt tgaattccgg
     3721 ttgcggtcct ttcaacactt tagaagccgc gacgcgctca tctttcatcg ccgaaagcat
     3781 ccgagcatag acggcttcgc cgatcatcgt tagcggtgcg ccagcatcca gtgaagccac
     3841 caccgtccat ttgccagtac ctttttgacc ggcggtatcc agaattttct cgaccagagg
     3901 ctgtccgtct tcatctttat aagccagaat atcgcgggta atttcaatca ggtagctttt
     3961 tagatcaccc tcattccaat cggcgaaaac ctgatgcatt tcggggtaac tcaagccgag
     4021 caggtctttc ataatttggt aagcttcgca aatcaattgc atatcaccat attcgatgcc
     4081 gttgtgaacc attttgacaa aatgaccagc gccgttttca ccaacccagt cgcaacacgg
     4141 tgtgccgtcc tcgactttag ccgcaatcgc ctgaaaaatc ggcttgacat gtggccaagc
     4201 ctccggtgaa ccacccggca taattgacgg accatttaac gcgccttctt ctccgccgga
     4261 gactccggta ccgatataca gaaaaccctg ctcctccaga tattttgtgc ggcgaatcgt
     4321 atccggaaaa tgcgaattgc cgccgtcaat aattatatcg cctttttcta aatgcggaat
     4381 tagtaattcg ataaaatcat ccaccggttg gccagctttt accatcagca taatcttgcg
     4441 tggttttttt aacgaggcaa taaattccgg aatcgagtga gtgccgataa tatttttgcc
     4501 cttgccacgc ccggccagaa atttgtccac tttttcgacg gtgcgattga aaaccgccac
     4561 cgtgaaacca tgccgctcca tattcagcac caaattctcg cccataacgg ctaatccaat
     4621 taaacctatg tcggctttat tcatttttta aatcctcctg atattataat ttaattatta
     4681 aatctttcct tggtagccca gagcccaaga gccggattcg aaatgtaaaa tacctcttcg
     4741 ccgtccggta gaatgttgaa accatcctgc attttcgccg gatcataacg tgccaacatt
     4801 ggcgccaatt cgccatatcg gaaattgacg ctttcgattt cttcccgact aatataaccg
     4861 gggcaatata ttaccgtaaa gcggttttcc gacgaaccgt gaatcagatg ggcggccgca
     4921 ctgagattat tctgtaagtc actattttcc tttacgaatt gcataacttc cggcgtggtg
     4981 cggtagccgt atttacgaat cagtcgatca atttccggat cctcaccaaa ttcacgtaaa
     5041 ccgggtgcca gcacgatcaa ttcaccaccg tcggcaatcg ccatacgagt ccgataaatg
     5101 cttttattgc cgagccaggt gctcttgaat tcctgcggat cgagataaac gacgactttc
     5161 ttgagcggtt tatccaacag atcgaagtta atagtcagcg acagttcgct cgccagctgg
     5221 aaacactcat aatcgtcgcc gataaataag cctttgatag ccatttgccc agtgctattt
     5281 tttccaatta ccgtcaagac atagacgatt ggcatttgcc gggcaaaatt atccgaagca
     5341 tagttcagca cgcggcgcac cggcgtatcg gcgcgtccca taatgcgttc tattccgtaa
     5401 accgcaccta aaaaatggct cttatttata ctttcatggc cgccggttcc aacgaaaatg
     5461 ttcttattat aatttgccat accaatcact tcatgtggca caacctgtcc aatggataaa
     5521 atcaaatcga agccgccctc gactaatagt ttattaacct gcgctggcca ttcataatcc
     5581 agttttcctt cggagacttc gaatacgaat tcaccgggca ccacgccgag ggtaaatagg
     5641 tcttctttcc agtgatgaat gcggaagagg tggcgcggca aatcaccgaa catcgcctcg
     5701 atttccgctt cggtcatcgg aaaatgtgtg ccgagcgccg gcaaaatcgc ctgtagattc
     5761 tttccataat attgaaatgc taaacgcgtc agttgaccgg cttgagaatg gaaacgggta
     5821 aaatccggcg gaacggctat cactttacgg cgcgctccca atttcgccag agctgaaaaa
     5881 aagccgtccc gaatctcggc ttccgtcagt ccgtaatcag ctgagcattt tgcaaaatac
     5941 aacattacag attgtcctaa aacagtttag cgctggatgc gggctgagcc gcctttaata
     6001 aaattttcta cttcggctaa agttgccatc gtcgtatcgc ccggataagt cgttacgaga
     6061 gctccgtgtg cccagccgag tttgacggct tcttcgggcg gaacattctt caataaacca
     6121 tagaaaaaac cggacgcaaa gccgtcaccg ccgccaaccc gatcataaac atccaattcc
     6181 gccgtcggcg cttggtaagt tgcgccattg atccaggcaa ccgcgctcca gctgtggcga
     6241 ttagtcgaat gaacttcccg caaagtagtt gccacaacct taatctgggg atattttttg
     6301 accacatcgc taatcatagc aataaaagcc gatggatcga gctttgactt ggcagttatt
     6361 tccgggccgg gaatccccag acctttctgt aaatcctctt cgttgccgac caacacatcc
     6421 acatacttga caatttcatt aatgacctcc accgctttct tttcaccgcc ccacaaattc
     6481 cagagtttgg cgcgaaaatt caagtcgaag ctggtgaccg caccagccgc cttagcggct
     6541 ttcatagcct cgataattac ctgcccggta gtttccgaaa gtgccgcaaa aataccaccg
     6601 ctgtggaacc agcgaacacc ttcggcgaaa atcgccttcc agtcaaaatc acctggtttt
     6661 aacaaagcgg cggcttcgtt cgaacgattg tagaaaacta ccggcgcgcg tacgccgtag
     6721 ccgcgatcac tgtaaacagt tgccatatta ggaccattca ccccattatg ggcaaaatgt
     6781 ttgtaaaagg gcttgacccc catcgcccgc acacgctcag caatcaaatc gccgatggga
     6841 taatccgcca tcgcggtcgc aatgccggta ttcagtcgaa agcaatctgc taggttagcc
     6901 gccacattat attcaccacc gctgacgtga attttgcatt cggtagcttt gcggaacggc
     6961 accgtgcccg ggtccaaccg atgcaccagc gccccgagtg atagcagatc gagactgccg
     7021 ttcggtttaa tattaagtcc gtaattcatt ttacaaaacc tctcatttta ataattgaaa
     7081 aattcaaacc ccgctgtaag cattaaaacc gccatccacc ggaataatca caccggttac
     7141 gaatctggaa ctgtttgaaa tcaagtaaat cagcgtccca attaagtctt ctggttcacc
     7201 aaagcgcccc atcggagtag cggcgataat tttacgccca cgttcggtca attcaccggt
     7261 ctccttgtcc gtcaacaaaa agcgattctg gtcggtgagg aaaaagcccg gcgctattgc
     7321 attgactcgg atatttgggg aatattcctg tgccatgtga accgccaacc attgcgtaaa
     7381 attgctaacg gcggctttgg cggctgaata agccggaatg cgcgtcagcg ggcgaaaagc
     7441 attcatagag gtgatattga taatattgcc gaacttctgt ttagccataa ttttgccgaa
     7501 aacctgactg gtcaaaattg ttccgagcaa attcaaatcc attgtaaaac gtaaagcctc
     7561 agctggcaaa tcgaaaaacg agcattctgg cgaagtcgtg gcgtctgggc gattaccgcc
     7621 ggcgccgttt atcaaaacat caacctgtcc aaactccgcc gtaactttag cggcggcatt
     7681 ttgtaggctt tctttcttta agacatcggt cggggtaaat aagatgcgat taccgctttt
     7741 acctggtgaa gtcgggatag atgttgtcgg cggttcacgc aaatcgagta ccgctacatt
     7801 cgctccggct ttaaatagct cggaaaccat cacacgtcct aaaatgccgg acccaccggt
     7861 gatgaccacg gttttgtcat ttaaattggt tttcattaat gcattctcct tttaaattaa
     7921 tatccaaata aacgtggtaa aaagagacta atctgcggaa aatatgtcac caataacaaa
     7981 gctaaaatca tagccgcata aaatggtaga attgacttta ctattttggg aattgtggta
     8041 ttggcaatcc cacatccgat aaatagaacc gatccaaccg gtggcgtgca taatccaatg
     8101 cacaagttca gaaccatgat tattccaaag tgaatcggat gaatgcccaa agcggttact
     8161 atcggcagaa aaatcggcgt aaaaatcaat accgccggcg tcatatccat aaaggttccc
     8221 acaaatagca gaatcaaatt aattatcagc agaatgacga ctttactgct ggataaggca
     8281 attaacgaag cggtaatggt ctgaggaata ttggcgtaag acataatcca cgacatagcc
     8341 atcgaagttc caatcaacag cataacaata gccgtcgtcg tgaccgtttc cagcagtacc
     8401 tgcggtagct ccttccattt gacttcatga taaatcaaaa ccgacaggat aaatgtataa
     8461 agtactgcga tcgccgaacc ttccgttgcg gtaaaaaacc cggcaataat tccgccgatg
     8521 acaatgacaa tcagcatcaa gcttggtaga gcatccagaa agcgcataat accttgtttg
     8581 aaagtatagc gctcgtcgct tccccgatat ccttttttca cggcaataat accagccgct
     8641 accattagca ataaaccaac cagaatcccc ggcagataac cggcgataaa aagagccgca
     8701 atggaaaccc cgccactcgc aagactatat acgatcagga tattactggg aggaatcaat
     8761 aatccagtcg tagctgaggt tatattaacc gatgcggaat aattggtgtc gtagccctct
     8821 ttttccatca gtggggtcat aaaaccgcca attgccgccg ccgccgccgc cgctgaaccg
     8881 gaaatcgctc caaaaaacat cgccgccaag atattgacat aagccagccc acccggccag
     8941 cgtcccacca acaccttggc aaaatcaatc aagcggcgcg caatcccacc ccgactcatt
     9001 aataagccag ataagataaa gaacgggatt gccagcaagg caaaactatc aataccggtc
     9061 gccattcgtt gggcaacagt cgtcagagcc ggcagagtat caatactgag caacatagtg
     9121 ctcagagtag cgataccaat actgaaagaa atcggcactc cagctaataa cagaaaaacg
     9181 aaacttgaaa ctagtactat tacttcagtc cactccatgg tattccgtcc ttttttcagg
     9241 tagattattt gttgaatcgg gtcgaaatag ctggccaaat aaaccaatta tttccacaat
     9301 tgagtaaatg gcaattaata tcccactaat aggtaaaacc atataaacat agcccagttt
     9361 taccttcaaa gccgccgata attggtttaa agtcaaggta atagttacta ggcgcaaccc
     9421 gccgataacc attaccgaga tggcaaaaaa cagaacacaa atctggataa atatctgcaa
     9481 tcgagccgca ttgttttttt tcattttggc actgaaaagg tcaattgcta ggtgcaattt
     9541 atgcccggag gcatatgcgg cgccgagtaa accaacccaa attagcagat agcgcgccag
     9601 ttcttccgtg taggaactcg gatgatgaag gataaagcgt gtaaatacct gccagagcac
     9661 atttaacacc gagatagcca ttaaaaccac tacgacccat tttaaaatat tatcgagtat
     9721 tttgcgtaac tttaacatta ttgcaccgct ctaattcttt ggattaaatc ataaatttca
     9781 ggttcgttcc gataactttc gtaaacgtcc ttgacttttt cctgaaatgg gcgtttgtcg
     9841 ggataactca caaccacgcc cgctttttgg acttcctcca gcgcttcctc cgtggcaatt
     9901 ttccacagtt cgcgctgata aatcgcggct tcatctgccg ctgattgcaa ccactcctga
     9961 gcttgcgggg gcaggctatt ccagaccttc gtgctaatca ataatatatc cggcacggca
    10021 gtatgttcat ccagcgcaaa atatttagcc acttcgtgat gatgtgatat gtaaaacgaa
    10081 ggtggattgt tttctgcggc gtccacaatg ccctgttgca gagccgtata aagttcgccc
    10141 caggcgattg gcgtagccga accgcccata gcctgaatca tctgaatgga agtcgtgctt
    10201 tcctgcgtac gaacctttaa acctcgtaaa tcctccggag tattgatcgg ctttttgacc
    10261 gaataaaaac tgcgacagcc ggcatccaga taacataatc cacgaatcca gtatttttcc
    10321 ccttcgagta aaagctcgcg accgatctcg ccttccagaa ccttaaaccg atgctcgtcg
    10381 tcgcggaaaa gatagggcat actgaaaact ttcattttat ccacaaaacc ttccagcaca
    10441 ctgcacgaga cctttgtcat tcccagactg ccgatctgaa gcaattctac atactcgcgt
    10501 tccgaaccca attgaccgct gggataaata tcaatacgat attttccacc ggatttttcc
    10561 gcaaagcgtt cgctcatata aaccaaggac ttgtgaacgg gatgtgaggt atccagtgca
    10621 tgcgccaatt tgatggtttt ggtgcgtgct tctgactcac aaccattcag gcaaaagcca
    10681 cttattatta ttgcggtacc tagtccaatt tttgttagaa atttagttct cataaagtta
    10741 ctcgatgtgt ttagagttta taagtttttt ttgccaaatc gtaggttagc gctctgatta
    10801 aaacgtgcgc ttcatctaaa tcaataatgt gtcgaacgac caaaccggct aggaaattag
    10861 aatctacccg acgacaaaga tcgtgccgcg ccggtattga cggaaaggat cgcgtatcat
    10921 cggtaaatcc cacggtatta taaaaaccgg cggtctccgt aatcatttcc cgaaagcgaa
    10981 tcataccctg aatactgtca tgaaaccacc aagccgcacc cagcttcata gccggataat
    11041 aaccggcgag tggcgctaac tcgcgggaat aggttgactc gtccaaagta aaaaccacca
    11101 gtgtcaaacg gctatcggta ccgtattcat tcagcagttc gcggagatta actgtgaact
    11161 ccgaacgcac cggaatatcg gctccggtat caggtccaaa tcgccgaaaa ttccgcaaat
    11221 tgtgatttcg cagagcgcca acgtgcatct gcatcactaa accgtcggct acactcatcc
    11281 gagccatctc cattaacata ttagcggtaa ataaacgtgc gtcttcggca ctagcttttc
    11341 cattacgcgc ccgttgaaaa atttcttcag cctgggacgc gctcaatcgg tgagtataag
    11401 cgctttcgac tccttgatca gtcgaaacgg cgcccattat cttgaaaaat tcgcgccgat
    11461 tttcgatagc gtcaatgaac ctaacataag tattgatttc atagccgacg acatccccca
    11521 gtttctcgat ttctcgatgc caacccactg catttatatt aaataaagcg tccggccgga
    11581 agcagggtac gattcttcct ttccatccgg attcctttat ctggcgatga tattttaggc
    11641 tatcagtggc gccgtcagtg gaagtcaaaa cctcgatatt aaatcgctca tataaagcgc
    11701 gcgggcgaaa ctccggcttc tgcagttgat tttgcaaatg atcataaatt gccatcgcat
    11761 tggcgctgtt gagcttttcg gtgactccaa aaacttcact aaattcataa gccagccaca
    11821 aactggtggg cgttccggca aatagataat aattatcggc aaaaatttgc cagatttttc
    11881 gatgatccgt ctcgaccggc gtgccgtcgc gagtcggaat tcccagcgat tccagtggaa
    11941 ttccttgaga atataaaagg cgataaatgt aatgatcagg caaaatgaaa agagccgtcg
    12001 gatccggaaa cggttgattt tcggctaaaa ttttagcctc gatatgccca tgcggcgaga
    12061 ttatcggcaa gtccttgact tgctggtaaa gttcacgtgc cagcgatcga atcgccgggt
    12121 cagaatcaaa aaaacggtca gaaggtaaaa tcataactcc tcataattgt attgtaattt
    12181 gcgacaggtt tcaccaatta ctaactggga tggaataaca atatgttgtt cgctacgtct
    12241 ctccgacgaa tgcaattcat ccaataataa attaaaggct ttctcaccga tggtactggc
    12301 tggttgatcc ataaaagtca atgctggcga cagataacga ttaaattgcc cacccccaaa
    12361 acaaactagg tcaatatcgc caggaatttt cagccccagt tcgctagcgg cttcgaaaat
    12421 gccgagagca actggaaaag ttaccgcaaa tatcaactcg ggtaaatctt tagtctcagc
    12481 cacttttttt aatgcttcat atccggctcg ctcaccaaaa ccaccttcga taatccaatc
    12541 tttgcgaatc ggcaaattat tgtcagataa tgcctgtcga aaaccagtct gacgttcgta
    12601 gccgatattg gtgtgactgt aaccggcgaa atgggcaatt ttgcgatacc cggcttgaat
    12661 gacataattc gtcaagcgcg ccgccgccgc tcggtcatca gtcacaacgt aattactttc
    12721 caaaccgaat ggaatccggt caaaaaatac aatcggtaca ccgcgctttt tgatatcttg
    12781 aaaaatgctg gtgtcagttg tattctcggt gactgaaact aataagcctt caactcgcat
    12841 cgccagtaaa gtctggatgt gtttgagttc atattccgga ttttcctggg atacggtcat
    12901 gattatatcg tactgactgg cattggcggc attgtaaatt gattcaatgc aagtagcaaa
    12961 aaaataatgt gctagcttag gtacgaccaa accaatcgtc cgtgaacttc tggctgaaag
    13021 attacgcgcc ataaaattgg gaacgtaacc caatttagcg gctagcgcct ggacttcgcg
    13081 cttggtggct gagccgatat cagggtgatc ccgcaacgcc ttagaaacag tgactttaga
    13141 aacctttaac tggcgagcga tatcatccag ggtaacgttt ggcgatctgt ttggcggttt
    13201 gttcactatt ttttcacttt accgttaact ttatcggtta agtaaaatgc aaatcttttt
    13261 ccaaattgtc aagtaaattt tttatgaatt cttcaatatt ttactgtttt ataagctaag
    13321 ttgttgataa tccgggtaat actgcacttg atttaagccg tcacccttcg acaagctcag
    13381 ggtgacattt ctggtttt
//
LOCUS       DAOSMP010000050        13364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_4650_length_13364_cov_23.421670, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000050 DAOSMP010000000
VERSION     DAOSMP010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 13364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13364
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4650_length_13364_cov_23.421670"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1421)
                     /gene="rho"
                     /locus_tag="PKX09_08175"
     CDS             complement(162..1421)
                     /gene="rho"
                     /locus_tag="PKX09_08175"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOU17837.1"
                     /translation="MENELTIAKLQSMKIKELSDMANQLEIPETTGLKKMDLIYKILE
                     ASAQKEGKIFAQGVLQVMDEGYGFLRSEKSSYLPGSDDIYISPSQIKRFGLRTGHIVC
                     GQIRPPKDNERFFALLRVDAVNMMDPEEVKARPVFDSLTPLYPQSRIQLETDSKNISM
                     RIMDLLCPIGKGQRGLIVAQPKTGKTTLLQQIANSITTNHPEITLMVLLIDERPEEVT
                     DMERSVKAEVISSTFDEPPERHVQVADMVLEKAKRMVELGFHVVILLDSITRLGRAHN
                     AVVPHSGKILSGGIDANALHRPKRFFGSARNIEGGGSLTIIATALIDTGSRMDDVIFE
                     EFKGTGNMELVLDRRLSDRRIFPSMDVNRSGTRKEELLLSAAELARVWILRKLLSEMT
                     PIEAMEFLIDRMNRTKSNREFLKSMNQ"
     gene            complement(2540..3484)
                     /locus_tag="PKX09_08180"
     CDS             complement(2540..3484)
                     /locus_tag="PKX09_08180"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU17838.1"
                     /translation="MNNYQPTVSVIVPAYNAAKTLLICLNSLLRQNYPQEKYSVIVVN
                     DGSNDTTGILFRQMNLPENFGIIEHPENRGLAAARNTGIQKSNAEIIIFLDSDMEVAP
                     DFISRHIAWYKRPEVVGIVSELQPAPESPYDKYQRYLYESKRGARVIGANEPLPFQYF
                     IFGITSVKRSALATVGGFNADIRQYGGEDTELAYRLWQKYPTGLFFDPEIIVIHHHYR
                     PFQQVLKIVEQFGRQVVPYIVEKHPELSRLYGMQFVKPLGGNNWKLKYWIGRTLQTNV
                     ALEILWFLFEIKPYPISNFLVRGLLASALLRGITSSRT"
     gene            complement(3489..4547)
                     /locus_tag="PKX09_08185"
     CDS             complement(3489..4547)
                     /locus_tag="PKX09_08185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU17839.1"
                     /translation="MKITFVLPGFIKIPMGGVKVVNEYANRLAEKGHEVTLVYPLALK
                     TGNRGYTIRKKISSLIDRIQRVPENLYYTPNPTVNVMVVRNTTSKYIPNGDAVIAVGW
                     QTAEAVTELPAEHGRKFYLLQSFETYFSQKKRILATYHLPLEKIAVSHWIIDELEKLG
                     EKCFGPLGNAINPTEFFIENPQPERSNDVLMIYHPHKIKGAKEGLAALQLIKRQFPTL
                     TAIIVAPRKPVHRIPSWIKVIIRPSISELRHLYNISKIFLHPSRWEGWPLPPLEAMAC
                     GCAVVATANRGVQEYLVDRENALLCPIDDVKTLVQNVITLLESDQLREELAKGGIKTV
                     QCYNWWECTSRLEQYLMG"
     gene            complement(4549..5628)
                     /locus_tag="PKX09_08190"
     CDS             complement(4549..5628)
                     /locus_tag="PKX09_08190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU17840.1"
                     /translation="MKILYISPENTVGTLTYWKRAHELNGNVCRYVTWYRSFGGYPED
                     ICLNLPLIAAQPWFIKPRLAFIRHIDRRDPYAALPGYPPYWNPPNWQKKLFRMREILW
                     QPIIDRAIREYGLEDFDVYHFEWGADFYRDARFAKKMKARGKKIICSYHGQDMRNRGV
                     IPEMDKISDLDLTSELDLTFRHPNLKYLFLPYDVLAFQPKNTPGKPLTICHATTNRRI
                     KGSDEIIRVCEELEQSHGIRFIFIENQPHSEVIELKKQADIYIDQITDHAWGYGMNSL
                     EALSLGLVCVTYLNPTYENFIPDHPFVNAGISNLKEKLLELIENPANLPARMAASREW
                     VERRHDYRNVVKELYRYYESIGVRF"
     gene            5947..7134
                     /locus_tag="PKX09_08195"
     CDS             5947..7134
                     /locus_tag="PKX09_08195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU17841.1"
                     /translation="MNIFIVPSWYPSKLSPENGTFFRDQAQILAKYGDDVTVIVDLVH
                     SFKNLLRIRRFSTVKFQPENLGGVIEYRQETLNWFPKRPQKTYFSQRRRLIKLLERAI
                     AERGRPDLVIAHSSLFAGAELARWLKDNDIPMIVTEHLMHFLKPEMLTEFYKDCIHEA
                     YCYADRIVATSSRLRDSIAVQFPEFPTKIVVVPNPVDISAFSLASEGKKALKPFVFLC
                     VALLRPEKRLDLLLRAFATVRQNNPDTQLVLVGDGPQRLNIEHLISNLNLQSSVTLTG
                     YLPQTEIAGWMQKCHTLILPSEVETFGLVLVEAMACGLPVIATRCGGPEDIVTPETGF
                     LVPVNDVTGLTEAMQKMIIEYCQFDPQSIRQTVIDKFSDHKYYWAIHNLYDEIMNNSP
                     KLK"
     gene            complement(7126..8580)
                     /locus_tag="PKX09_08200"
     CDS             complement(7126..8580)
                     /locus_tag="PKX09_08200"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOU17842.1"
                     /translation="MSGNFRKFFKESTLYLGGLVLNRLVSFLLLPIYTNVFDKTQNGI
                     ITIAYAFLGFMVIILPYGTDAALLNFYTGNDREGKNYFSSAYSLVLISNLLILGLAFL
                     ARNSLSQAVLRVSDPHIFVWCLIILFFDTLNGLTLLVLRAEGRPLTFISFSLANVLLA
                     MGLNIFLVVHRRLGLAGIFYSNILTSGMIWLGLLPLVLKRLDLRLISGKYIRNLVRFG
                     FPFLPAGIFAMILDLSDRWFLEYFTDMETVGLYGVGYKLAAVMLVFVLAFNSAWQPFF
                     LKARREQNTPNIIARVSFFVIGGLAFCWLFFSIWMNYIVRIKISGRYLIGAEFWAGTS
                     ITPLIMLAYFFQALYLLQLPSIFLLEKTKWEPVFRGLGAATNIILNIVFIPRLGMSGA
                     AIATVLAYLAMVIAIWFFNRKYYPIKYDWYKIGAIIVLTAAAYLVAIWGDSVIYRVIA
                     TGIGLLGIGGILLLPLKGRILPILKKKVTNKNSL"
     gene            complement(8583..>10099)
                     /locus_tag="PKX09_08205"
     CDS             complement(8583..>10099)
                     /locus_tag="PKX09_08205"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOU17843.1"
                     /translation="SAEKFVYLGVFRYLINMTSSIRSLTKQTLIYGVGTILARLVTFL
                     LLPLYTNILPAAEYGLASLVFAFNGFMIIIFNYGLDSAMMRYYGESTEPAVRKRLLST
                     SIWLTISTSLILSGLIYGYSGFLGRVLLGDAARGELLRYSAGILFFDAIAHVPFALLR
                     LEERPIRFMSIRLLNVIMMFGLNIYFVAHLQRGVAGIFQGNLITSVITTTILFILIIP
                     RLSLQFSGESARNLVLFGLPFIPAGLATVCMEMLNRYILEHLMGLEAVGVFSAGFKLG
                     IFMLLLTNAFYYAWQPFFLKAGPQESSRLLFARIFTYFVLVELIFWLVLTALMPEIVR
                     FHIGQIYLIGKEYQSCEPMVPIILMGYVFLGIYQMFLPGIYFEKKTRYLAYLTIFAAL
                     VNVGFNFILIPSLGILGSAFSSLIGYFTLAVCSYFVSQRLFRVPYELRRVGLLFLLTL
                     AGGIPIWLFRPNLTIRIAILVVYPLLLWLAGFFKSSEIAKLKQIFSKTASHHKN"
     assembly_gap    10100..10199
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10363..11331)
                     /locus_tag="PKX09_08210"
     CDS             complement(10363..11331)
                     /locus_tag="PKX09_08210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU17844.1"
                     /translation="MSITVEIKQDFPDDQWDRFVYQSVNGTIFHTCQFLSYHPKGRFE
                     DASLIFRQEAELFALFPATRGNWDGYDTLWSHRGASYGGFVYREGLSIKNAYELVEKL
                     IEYARRERIKRIVLTLPPIIYNRRLSNYIDFALIQHGFQYLKREVSSIVSLEENIDLN
                     VAKFKPSNRTAFRRAQKLGVVVRESQDYANFYKILKKNLKIRHGVQPTHTLEELVQLK
                     DLFPEKIHLFGAYLADEMVAGVVMFDCNPQVSLAFYISHNEERQECRGVNLLFYEIIK
                     RCIAQGFKFLDFGIFTVNMEPNFGLARFKEGFGSSGILRDTLYLDL"
     gene            11591..12871
                     /gene="eno"
                     /locus_tag="PKX09_08215"
     CDS             11591..12871
                     /gene="eno"
                     /locus_tag="PKX09_08215"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOU17845.1"
                     /translation="MSTIIEVNAREILDSRGNPTIEVEVLTESGFYGSAAVPSGASTG
                     EHEAVELRDGDKSRYLGKGTLKAVQNVNDIIAPEIIGEDVLDQAWIDNLMIELDGTPN
                     KAKLGANATLGVSLAVARAAANFVGLPFYKYIGGVNARVLPVPMMNILNGGKHADNNV
                     DLQEFMIFPVGAKCFAEALRMGVETFHQLKSVLKKKGMNTAVGDEGGFAPNLRSNEEA
                     IEVILEAAAKTGYKIGSELFIALDPASSSFYNTETKKYELKSENRSLSSEEMVDYYKN
                     LVDKYPIISIEDGMAEDDWDGWKLMVKKLGNRIQIVGDDLTVTNVERLSRAIKEKAIN
                     SILIKLNQIGTLTETLNAIELAHRSGFTTVISHRSGETEDTTIADLAVAVNSGQIKSG
                     SACRTDRICKYNQLLRIEEELADDAIFGGTRLFK"
     gene            12868..13215
                     /locus_tag="PKX09_08220"
     CDS             12868..13215
                     /locus_tag="PKX09_08220"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOU17846.1"
                     /translation="MRKSRPKSIKSKDGSGYLHKLYWILGILCAITLFIIFFVIGDYG
                     LYQIYLLNKQKEQIENHLVELQYTQDSLLIEKTRLESDLQYIEKLAREKYRMAKKGEK
                     VFRVIEKPVERLN"
BASE COUNT         4003 a         2995 c         2707 g         3559 t
ORIGIN      
        1 cttcataatt taccatttag ggctccaaat tgatttgtta aatgcatagg ttttgccaaa
       61 tatcacccta aatatataca tttatatcaa atatagcaag cgataaatat ctgttattac
      121 aatatattag atactgtttc agaaagccct ataatagccg tttattggtt catagatttg
      181 agaaattcac gattgctctt ggtgcgattc atacgatcaa tcaggaattc catggcttcg
      241 attggagtca tttcgcttaa taatttccgc aaaatccaga ctcgtgccag ttccgccgct
      301 gaaagtaaga gttcctcttt gcgcgtaccg gaacgattga catccattga agggaaaata
      361 cggcgatcgc ttaaccggcg atccagtact aattccatat taccggtgcc cttaaactct
      421 tcaaagatta cgtcgtccat acgggaaccg gtatcaatca gagccgtggc gataattgtc
      481 agactaccgc cgccttcgat atttcgggca gaaccaaaaa agcgtttggg gcggtgtaga
      541 gcgttggcgt caataccacc ggataggatt ttaccactgt ggggaacgac ggcattatgg
      601 gctcgcccaa gacgggtaat actatccagc aaaatgacaa catgaaatcc cagttcgacc
      661 atacgcttgg ctttttctaa gaccatatct gccacttgaa catgacgttc cggtggttca
      721 tcaaaagtcg aactaatgac ctcggctttg acggaacgtt ccatatcagt gacttcctcg
      781 ggacgttcgt caattagcaa taccatcaac gtaatttcag ggtgattggt agtaatacta
      841 ttggcaattt gctgaagcaa agtcgttttc ccggtttttg gttgagcaac aatcagtcct
      901 ctctggcctt tgccaattgg acaaagcaaa tccataatgc gcatcgagat gttttttgaa
      961 tcggtttcga gctgaattcg gctttgggga tataaaggcg tcagggaatc aaaaaccgga
     1021 cgagctttta cctcttccgg atccatcata ttaaccgcat caacgcgaag aagtgcgaaa
     1081 aaacgttcgt tgtctttagg aggccgtatc tgtccacaaa caatatgacc agttcgcaaa
     1141 ccaaatcgtt tgatttgaga aggtgatatg tatatatcat cagaaccagg taaataactc
     1201 gatttctcag agcgtaaaaa tccatagcct tcatccatta cctgtagaac accctgggca
     1261 aatattttac cttctttttg agcactggct tcaagaatct tataaatcaa atccattttt
     1321 ttaaggccag ttgtttctgg aatctccagc tgattagcca tatcggacaa ttctttgatt
     1381 ttcatcgatt gcagttttgc aatggtcaac tcattttcca tagttattcc ttttttaaag
     1441 gttttacacg aatcgttagt ctaaatagag gtaggtcaaa aaattaattg attattaatt
     1501 aatgtctcta atttaaatag tcaaattcat aagtcaaata taatttttct cagcagtata
     1561 ttaaatggac attagatcag aagatcgcgt ttactcgtaa tcagtaattt ttcactctca
     1621 aattcagctt tttttggcta aagctaagat tggggctgaa caatgagact tgactgtatt
     1681 atcaaaaaac ctgaatcaat cttaaaactt tcttagcagg cttgagtcga gttggatttt
     1741 atgaatttcc ggtagatgct cactcatcgc agactcccac gaagccttcg gagaattagt
     1801 gggttgttct aacttgaatc aaaacttcaa catagatacg agatatgaag ggcgattggc
     1861 taaatcgcta ccttggcact ggtcgagtcg ccaactcgac catcattatc tttttacttt
     1921 acctaagcct tttcactaat tgccaatgcc caatgcccaa tgacccttcg cttcccagcc
     1981 taaaagaaag gttaaggctg agaaaaaagg ctaagatttg aattctctct cagtctcttt
     2041 tctatttact cagcctcttt accaatgact aatgaccaat gactaacctt cctgttcttt
     2101 tttcctttgc cgaaaaaaga accaaaaaag gcaacggcta aagaacatct cacgggggtt
     2161 cgctcagtcc cagcgaatgg aatttaaggt gcaaaagtta tcaagttcat ataacattag
     2221 tgacatagga aaaggttatg gataattatg aaagtgctat taccgcgctg aaaattccat
     2281 tttttccgct gggacttcgc ttcccagccc gagatgttct taatgccgta atagtaaatt
     2341 gaacgatcag gtggggcgct cgtcattact caacctaaaa gaaaggctaa gactaagaaa
     2401 agaggttaag gctgagaaaa aagactaaga tttgaattct ctctcaacct caatctcttt
     2461 tctcaacctc tttcctcagt ctcagtctct ttactcagcc tctttaccaa tgactaatga
     2521 ccaatcccgc cgccttaatt caagttcggg aagaagtgat ccctcgcagg agcgccgagg
     2581 cgagtaagcc gcgtaccaga aaattagaaa ttggataggg ctttatttca aatagaaacc
     2641 agagaatttc taacgccacg ttagtttgca gagttcgccc aatccagtat tttagcttcc
     2701 agttgttacc gcccaagggt ttgacgaatt gcatgccata caatcggctc agttccggat
     2761 gcttttccac gatataaggc acaacctgtc tgccaaattg ttcgacgatt ttcagtacct
     2821 gttggaaagg gcgatagtgg tgatgtataa ctataatttc cggatcgaag aacaaccctg
     2881 tcggatattt ctgccagagt cggtaagcca gctcggtgtc ttcgccaccg tactgccgaa
     2941 tatcagcatt gaaaccgccg acagtcgcca gtgccgatcg cttaactgag gttattccaa
     3001 aaataaaata ctgaaacggc agtggctcat tggcgccgat aacgcgtgct ccccgcttac
     3061 tttcgtacag atagcgctga tatttgtcgt atggactttc cggcgctggt tgcaactcac
     3121 tcactattcc tacgacctcc ggtcttttat accaggcaat atgacgactg ataaaatccg
     3181 gtgctacttc catatcggaa tccagaaaga ttatgatttc agcattcgat ttttggattc
     3241 cggtgttgcg cgcggcggct aagccgcggt tttctgggtg ttcaattata ccgaaatttt
     3301 ccggcaaatt catttgtcga aataatatac cggtagtatc attcgaacca tcgttaacaa
     3361 ctataaccga atatttttct tgtgggtaat tttgccgtaa caatgaattt aagcaaatca
     3421 gtagagtctt agcggcatta taagccggaa cgataacgct gacagttggt tgataattat
     3481 tcaggctatt accccattaa atattgttcg agtcggctgg tgcactccca ccaattatag
     3541 cactggacgg tcttaatacc accttttgcc agttcctctc gcagttgatc tgattcaagt
     3601 agggtgatta cattttggac taaggttttg acatcatcaa ttggacatag aagagcgttt
     3661 tcccggtcaa ctaaatattc ctgcacaccg cgattggcag ttgccactac cgcacaaccg
     3721 caagccatag cttccaaagg cggtagaggc cagccttccc agcgactcgg atgaagaaat
     3781 attttactta tgttgtataa atgcctcaac tcgctgatgg acggccggat aattacttta
     3841 atccaggaag ggattcgatg aaccggctta cggggtgcaa cgattattgc ggtcaatgtc
     3901 gggaattgtc tcttgattaa ttgcaaagcc gctaaacctt ctttggcgcc tttgatttta
     3961 tgcgggtgat aaatcatcag cacgtcgtta ctacgttctg gttgagggtt ttcaatgaaa
     4021 aattcggtgg gattaattgc attaccgagc ggaccaaagc atttttcacc aagcttttcc
     4081 agctcgtcaa tgatccagtg cgacactgca attttttcca gaggcagatg ataagttgcc
     4141 agaatccgct ttttttgcga aaaataggtc tcgaaacttt gcaataaata aaatttgcga
     4201 ccgtgttcag ccggtaactc tgttacggct tccgccgttt gccatccgac ggctataacg
     4261 gcatcgccat tcgggatata tttcgatgta gtatttcgca ccaccataac gttcacggtc
     4321 ggattcgggg tataataaag gttttcgggg acgcgctgaa ttcgatcaat taatgaggag
     4381 atttttttgc gaattgtata gcctcgattt ccggttttca gagctaacgg ataaaccaga
     4441 gttacttcat gccccttttc agccaggcgg ttggcatact cgttgacgac tttgaccccg
     4501 cccatcggaa ttttaatgaa accaggtaaa acaaaagtta tcttcatatt agaatcgaac
     4561 tccgatggac tcgtaatacc gatacaattc ctttacgaca ttccgataat catgccgcct
     4621 ttccacccat tcgcggctag ccgccatccg agccggcaaa ttggctggat tttcaatcag
     4681 ttccagtaat ttttctttca ggttggaaat accggcattc acaaacggat gatctggtat
     4741 aaaattttcg taagtgggat ttaaataagt cacgcaaacc aatcccagcg aaagtgcctc
     4801 caatgaattc ataccgtagc cccaggcatg atcggtaatc tggtcaatgt aaatatcagc
     4861 ctgtttcttc agctcgatca cttcggaatg tggttgattc tcgatgaaaa taaaacgaat
     4921 accgtgactc tgttcgagtt cctcgcagac gcggataatt tcatcggagc cttttatgcg
     4981 gcggttggtg gttgcatggc agatcgtcag cggtttgcca ggcgtatttt tcggctgaaa
     5041 agccagcacg tcatacggta aaaaaagata tttcaagttc ggatggcgaa aggtcaaatc
     5101 cagttcgctg gttaaatcca agtcgctgat tttatccatt tccggaatga cgccgcggtt
     5161 gcgcatatcc tgtccatgat aggagcagat gatttttttg ccgcgtgcct tcattttttt
     5221 agcaaaacgc gcgtcgcggt aaaaatctgc gccccattcg aaatggtaaa catcaaaatc
     5281 ttccaagcca tattcccgta tggcacggtc aataatcggt tgccacaata tttcgcgcat
     5341 tcggaaaagc tttttctgcc agttcggcgg attccagtaa ggtggatagc cgggtaatgc
     5401 ggcgtatgga tcgcggcggt ctatatggcg aataaaagcc agtcgcggct taataaacca
     5461 cggctgggcg gcaatcagtg gcagattcaa acaaatgtct tccgggtaac cgccgaaact
     5521 gcgataccag gttacatacc ggcagacatt gccgttcaat tcatgtgccc gtttccagta
     5581 agtaagcgtg ccgacagtat tttctggtga aatatataga atcttcaaca cgattgaaaa
     5641 ataagcaatt caggattaat tgggaatcga aaagttctac aattaaaatt ccttcaaatt
     5701 aattttaaaa cctattccac ttagatttta tggtatattg gtttgcaaat ggtgagtata
     5761 ttggtaaatc atattttaca aattgtaatg tttttataaa ttaaataagt agtggaaaac
     5821 caaaatgata tataaaccca gaaattccca gcgctgtatg aattattcaa gactcgtaat
     5881 ttcatcaggg gtttgccgac tgacacttta taattatctc aactttatct aggctgaaga
     5941 aatacagtga acatcttcat tgttccgtcg tggtatccat caaaactctc gcctgaaaac
     6001 ggcacattct tccgcgacca ggctcagata cttgcgaaat acggcgatga tgtcacggta
     6061 atcgtagatc tggtgcattc ttttaaaaac ttgctgcgga ttagaagatt tagtactgtc
     6121 aagttccaac cggaaaatct cggcggcgtt atcgaatatc gtcaggaaac cttaaactgg
     6181 tttccgaaac gacctcaaaa aacttatttt tcccaacggc ggcgcttgat caaactactt
     6241 gaaagggcga ttgccgagcg cggccgaccg gatttagtca tcgctcactc gtcattattt
     6301 gccggagcag aactcgctcg gtggctgaaa gacaacgaca tcccgatgat tgtcaccgaa
     6361 catcttatgc attttttgaa accggaaatg ctaactgaat tctataaaga ctgtatccac
     6421 gaagcgtatt gctacgccga ccgaatcgtg gctaccagtt cccgattgcg ggattcgatt
     6481 gcggttcaat tcccagaatt tcccaccaaa atcgtcgttg tcccgaatcc ggtagatata
     6541 tcggcttttt ctctcgcttc cgagggaaaa aaggcactaa aaccatttgt ctttctttgt
     6601 gtggctcttc tgcgccccga aaaaaggctc gaccttctgc tgagagcttt cgccaccgtt
     6661 cggcaaaata atcctgatac ccagcttgtt ctcgtaggag atgggccgca acgtctaaat
     6721 atagagcacc taatttctaa tttgaacctg caaagttccg ttactctgac cggttatttg
     6781 ccgcagaccg aaattgctgg ctggatgcaa aaatgccaca cgctaatttt acccagcgag
     6841 gtcgagactt ttggcctggt tctggtcgaa gctatggctt gcggtctgcc agtcattgca
     6901 acccgctgtg gtggcccgga agatattgtc acccccgaaa ccgggttttt ggtgccagtt
     6961 aatgacgtta ctggtctgac cgaagcaatg caaaaaatga ttatcgaata ttgtcaattc
     7021 gatcctcaat ccattcgaca aacagttata gataaattca gcgatcataa atattactgg
     7081 gcaattcaca atctctatga tgagattatg aataactctc ctaaactaaa gtgaattttt
     7141 gtttgttact ttttttttca gaatcggcaa tattctaccc ttcaaaggca agagaagaat
     7201 tccaccaatt cccaataacc cgattccagt agctattacc cggtaaataa ccgaatctcc
     7261 ccagatagca accagataag ccgccgcggt caaaacaatg atcgctccaa ttttatacca
     7321 atcgtatttt atcggataat atttccggtt aaaaaaccag atggcaatta ccatcgcaag
     7381 atacgccaga acagtcgcaa tcgccgcccc gctcattccc aggcgcggta tgaacacaat
     7441 atttagaatt atattagtag ccgcgcctaa tccgcgaaaa accggctccc atttagtttt
     7501 ttccagaaga aaaatactcg gtaattgcaa caaataaagt gcttgaaaaa agtaagccag
     7561 cattatgagc ggcgtgatac tcgtaccagc ccaaaattcc gcaccgatca aatatcggcc
     7621 ggaaatttta atcctcacga tataattcat ccagatacta aaaaacaacc agcagaaagc
     7681 caatcccccg ataacaaaaa aggaaaccct ggcaataata ttaggagtat tttgctcacg
     7741 ccgcgctttc aggaaaaagg gctgccaggc ggaattaaac gccagcacga aaaccagcat
     7801 aaccgcggct aatttatagc caacgccata taatccaacc gtttccatat cggtaaaata
     7861 ttcgaggaac cagcgatcgg ataaatccaa aatcattgcg aaaattcctg ccggtagaaa
     7921 ggggaacccg aacctgacta aattcctgat gtatttgcct gaaatcaagc gtaaatccag
     7981 ccgtttcagt accagcggta aaagtcccag ccagatcatt cccgatgtta gaatattact
     8041 gtaaaaaatt cctgccaagc caagcctgcg atgcactacc agaaaaatat tcaatcccat
     8101 tgccaacaag acatttgcca aactgaacga gatgaaagtc agcgggcggc cttccgctcg
     8161 taagacgagc aaggtcaagc cattcaaggt atcgaagaat aaaataatca agcaccaaac
     8221 gaaaatatgg ggatcgctca cgcgtagtac ggcttgcgat aggctgttgc gcgctaaaaa
     8281 agctaatccc agaattagca aattcgaaat cagaactagc gaataggcac tcgaaaaata
     8341 attcttacct tcacggtcat ttccggtata gaaattcagc agggcggcgt ccgtgccata
     8401 cggtaaaata atcaccataa atcccagaaa agcataagca atcgttataa tgccgttctg
     8461 agttttatcg aaaacattgg tgtaaattgg aagtaataga aaacttacca ggcgatttag
     8521 cactaagccg cctaagtaaa gcgtcgattc tttgaaaaat tttctgaaat tacccgacat
     8581 attcagtttt tatgatgcga ggcagttttg gaaaatatct gtttcaattt cgctatctcg
     8641 gaactcttga aaaaaccagc cagccataaa agaagcggat aaaccaccag aatagcgatt
     8701 ctaattgtta gattcggtct gaatagccaa ataggaatcc caccagccag agttaataga
     8761 aacaaaagcc caactcgccg caattcataa ggcacccgga aaaggcgttg cgaaacaaaa
     8821 taactgcata cagctaaagt aaaatatcca atcaaggatg aaaaagccga accgagaatt
     8881 ccgaggctcg gaataagtat aaaattaaag cccacattta ccaaagccgc aaaaatagtc
     8941 aggtaagcca gataacgggt cttcttttca aaataaattc ccgggagaaa catctggtat
     9001 attccaagaa aaacatatcc catcaaaata atcggcacca tcggctcgca agattgatat
     9061 tccttaccga ttaaataaat ctgaccaatg tggaaacgca caatttccgg cattagagca
     9121 gtcagcacca accagaaaat cagctcaacc aacacgaaat aggtgaaaat ccgagcaaat
     9181 aacaggcgcg acgattcctg cgggccagct ttcaggaaaa agggttgcca ggcgtagtaa
     9241 aatgcgttcg ttaaaagaag cataaatatt cccaatttga aaccggcgct aaaaacgccc
     9301 accgcctcca aacccatcaa gtgttccaga atataacggt taagcatttc catacaaacc
     9361 gtagctagcc cggcgggaat gaaaggcaga ccgaaaagta ccaaattgcg ggcactttct
     9421 cccgaaaact gcagacttaa ccttggaata attaaaatga aaagtatagt agttgtaatg
     9481 acagatgtta tcagattacc ctgaaagatt ccagctactc cacgctgaag atgagcgaca
     9541 aaataaatat tcagcccgaa catcataata acattcagaa gccgaatact cataaatcga
     9601 atcggccgtt cttcaagacg tagaagcgca aacggcacgt gcgcaatggc atcgaaaaaa
     9661 agaattcccg ccgaataacg caggagttca ccacgcgccg cgtcgcctaa taaaacgcgc
     9721 ccgagaaatc cactgtaacc gtaaatcaaa ccactcagaa tcaggctggt cgagatcgtt
     9781 aaccagatcg aagttgataa caagcgcttc cttacagccg gttcggtgct ttcgccataa
     9841 tagcgcatca tcgccgaatc cagaccgtaa ttgaaaatga tgatcataaa gccgttgaaa
     9901 gcaaagacca gagacgccaa accgtattcg gctgccggca gaatattcgt atataatggc
     9961 aatagcaaaa aagtcacaag ccgggctaaa atggtaccga cgccataaat caaagtttgc
    10021 ttggtgagac tacgaatact tgaagtcata ttaattaaat atcgaaaaac gcccaaatat
    10081 acgaactttt ccgcagaatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    10201 tcatattaat taaatatcga aaaacgccca aatatacgaa cttttccgca gaatcagcgc
    10261 caactgtagg acaggcattc ttgcctgtct attcctattc accaagtact tactaattcc
    10321 ccgccgtttc cttgctttat tataaaaccg ctaaaattta ttctaaagat cgagatataa
    10381 tgtgtcccgc agaatgccgc tcgaaccaaa tccctcctta aatcgagcta gtccgaaatt
    10441 cggctccata tttacagtaa aaataccaaa gtcgagaaac ttaaaaccct gcgcaataca
    10501 tctttttata atttcataaa aaaggagatt gacgccgcga cattcctgcc gttcttcgtt
    10561 gtggctaatg taaaaagcca gtgatacttg cggattacaa tcgaacatta ccactccggc
    10621 gaccatttca tcggccagat aagccccgaa cagatgaatc ttttcgggaa atagatcctt
    10681 taattgcaca agttcttcca atgtatgagt tggttgaacg ccgtggcgga ttttgaggtt
    10741 tttctttaaa attttataaa aattggcata atcctgcgat tcccgcacga ccacgcctaa
    10801 tttttgcgcc cgccggaaag ccgttcggtt agatggttta aatttcgcga cattcaggtc
    10861 gatattttct tccaatgaaa cgatactcga aacttcacgc ttcaaatact ggaagccgtg
    10921 ctgaatcagc gcaaaatcta tataattgga taaacgccgg ttataaataa tcggtggtag
    10981 agttagcacg atgcgcttga ttcgttcccg tcgcgcatat tcaatcaatt tttccacgag
    11041 ttcataagca tttttaatac taagcccttc gcggtaaaca aatcctccgt aacttgcccc
    11101 gcgatgcgac cagagggtat catagccgtc ccaattcccg cgcgtcgccg gaaatagtgc
    11161 aaacagctcc gcctcctgac gaaaaatcag cgaagcatct tcgaagcgcc ccttgggatg
    11221 atacgataga aattggcaag tatgaaagat agtcccattg acgctctggt aaacgaacct
    11281 atcccactga tcatctggaa aatcctgttt aatctcaacc gtaatggaca ttttatttcc
    11341 tcaaaaaagt cggcttgaat ttaaattcaa ctaccggcta aatcaaatcc aattcacaaa
    11401 acggacattt catcatataa tctccggatg aaattttata agattgtcgg tgattatagg
    11461 caattttttg atgatttctt aattttcttt ggtcggcgtt cgcactttta ttgaaaaata
    11521 tccgagagct gattaaattg acagcgtatt tgaattatct taaaaaatcg aagcaaataa
    11581 ggagtaatta atgagtacaa ttatcgaagt aaatgcccgt gaaattctgg actcgcgtgg
    11641 taatccgacc atcgaagtag aagtcttgac tgaaagcggt ttttacggca gtgccgccgt
    11701 cccgtccggc gcttccactg gcgaacacga agccgtggaa ttacgcgacg gcgataaatc
    11761 gcgctacctt ggcaaaggca ctttaaaagc cgtgcaaaat gtcaacgaca tcattgcacc
    11821 cgaaatcatc ggcgaagatg ttctggatca agcttggatc gacaatctta tgatcgaact
    11881 cgatggaaca cccaacaaag ccaaactcgg cgccaatgca actctcggcg tttccctggc
    11941 cgtagctcgg gcggcggcta atttcgtcgg cttgccgttc tataaataca tcggcggcgt
    12001 caacgcccgc gttctgccgg ttccgatgat gaacattctg aacggcggta agcacgccga
    12061 caataatgtt gatctacagg aatttatgat tttcccggtc ggcgccaaat gtttcgccga
    12121 ggctctgcgg atgggcgtgg aaacctttca tcaattaaaa agcgtcttga agaaaaaagg
    12181 tatgaatacg gccgtcggag atgaaggcgg atttgccccc aatttacgca gtaatgaaga
    12241 agccattgag gtgattttag aagccgccgc caaaaccggt tataaaattg gcagcgaatt
    12301 attcatagct ctcgacccag cttcatcctc gttttacaac actgaaacga aaaaatatga
    12361 actaaaatcg gaaaatcgca gtttgtcttc tgaagaaatg gttgattatt ataaaaattt
    12421 agtggataaa tatccgatta tttccattga agatggtatg gcagaagacg attgggacgg
    12481 ctggaagcta atggtcaaga aactcggcaa tcgaattcaa atcgttggcg atgatctgac
    12541 cgttaccaat gtcgagcgtc tatcgcgcgc catcaaggaa aaagccatca actcaatatt
    12601 gatcaagctg aatcaaattg gtactttgac tgagactctg aacgctatcg agcttgccca
    12661 tcgcagtgga ttcactaccg tcatttccca tcgtagtggc gaaaccgaag acacgactat
    12721 cgctgacctg gcggtggcgg tcaattctgg ccagattaaa agcggttctg cctgccgcac
    12781 agaccgcatt tgcaaataca accagctcct gcgaatagaa gaagagctgg ctgacgacgc
    12841 catttttgga ggaacgcgcc tatttaaatg agaaaaagcc gtccaaaatc tattaaaagt
    12901 aaagacggtt ccggttattt gcacaaactt tactggattc tcggaatact ttgtgcaatt
    12961 acccttttca tcattttttt tgtaattggc gattatggtc tttaccaaat ctacttactc
    13021 aacaagcaga aagaacagat tgaaaatcat ctagtagagt tgcaatatac gcaggattct
    13081 ttattgatcg agaaaacccg attggaaagc gacctgcaat atatcgaaaa attagcccgt
    13141 gaaaagtatc gaatggctaa gaaaggcgaa aaagtctttc gcgttatcga aaaaccggtt
    13201 gagcgtctaa attaaacgag cgcgattact gctctgaaaa ttggcgttaa atcacggaat
    13261 ttatatcttt caccagttga attggtaaat taaagcccgt tctcacaacc tgcgggcgct
    13321 ttgaaggagg aaaatatgga aaagcgattt ttattttgct tgtt
//
LOCUS       DAOSMP010000051        13262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_4707_length_13262_cov_17.492163, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000051 DAOSMP010000000
VERSION     DAOSMP010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 13262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13262
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4707_length_13262_cov_17.492163"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PKX09_08225"
     CDS             <1..471
                     /locus_tag="PKX09_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU17847.1"
                     /translation="LDDSVRSEFKSFKFKTNKKDVMNVRVKLTLSTNIKGLEGMFIDE
                     IGTVNENTLAKIAIEQIMLEQPDVPESYSLAQNFPNPFNPTTTISYSLPKVGNVTITV
                     YNLLGEEVATLVKGERQEGRHSITFDGSRLASGEYIVRMQSGNYSKTIKILLMK"
     gene            800..1237
                     /gene="rplM"
                     /locus_tag="PKX09_08230"
     CDS             800..1237
                     /gene="rplM"
                     /locus_tag="PKX09_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006854802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOU17848.1"
                     /translation="MYKTSFPKVTEIKQDWYVVDAEGVVLGRLASKVAAILRGKNKPY
                     FTPHLDTGDYVIVINADKVRLTGRKAEIKTYFKHSGYLGHERFLKYKDVFQASPEEII
                     ERAVKGMLPHNALGRQLFRKLKVYRGPVHPHQAQQPKPLVIEG"
     gene            1245..1637
                     /gene="rpsI"
                     /locus_tag="PKX09_08235"
     CDS             1245..1637
                     /gene="rpsI"
                     /locus_tag="PKX09_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOU17849.1"
                     /translation="MPKLEYVSLGRRKSSIARLRMQTGEGKVIINSRPLDEYFGRESL
                     KRMIIQPFEVCGLTGQFDVNVKVEGGGYTGQAGAIRLAIARALEQYDPELRAKLKPAG
                     FLTRDSRVKERKKYGLRGARRAFQFSKR"
     gene            1678..2382
                     /gene="rpsB"
                     /locus_tag="PKX09_08240"
     CDS             1678..2382
                     /gene="rpsB"
                     /locus_tag="PKX09_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOU17850.1"
                     /translation="MLMKSGAHFGHLTRKWNPKMKDYVCMTQNGIHIIDLNKTLACLD
                     EATRFIREVVRKGGDVLFVGTKKQAKEIVQREADRCGMFYVVERWLGGTLTNFSTIKR
                     SIRHLQKLEKDKSIGYGENLTKKEKLALERERIKLADLHRGIKDMRKLPDVLVVVDTT
                     YDNIAVQEARRLDIPIIAILDTNADPTIVDYPIPANDDSIQTINLIITELADAVIEAK
                     NSRLVEVPPPVEEAES"
     gene            2406..3011
                     /gene="tsf"
                     /locus_tag="PKX09_08245"
     CDS             2406..3011
                     /gene="tsf"
                     /locus_tag="PKX09_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOU17851.1"
                     /translation="MGITATQVKELRDRTGLGMMECKNALVESDGNIDKAVEILRKKG
                     IAKAEKKADRVAKEGVILSYIHPGNKLGVLIEVNCETDFVAANTDFINFVKDIAMHIA
                     ATNPLSIRREEIDPAILEKEKEIYRAQVRAQNKPENIVERIVEGKVEKFYQENCLLEQ
                     PFVKNPQITVKDYVTSIIAKTGENINIARFVRFQLGESFEK"
     gene            3034..3759
                     /gene="pyrH"
                     /locus_tag="PKX09_08250"
     CDS             3034..3759
                     /gene="pyrH"
                     /locus_tag="PKX09_08250"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOU17852.1"
                     /translation="MASIPYKRVILKFSGEALAGEKQVNFDPDIIRQIAGEIKSAADL
                     GVQMGIVFGGGNIIRGSSGVGKSLDRIKADYMGMLATVINALAMQDALEKLGLRVHVF
                     SAIQMIEVAELMIIRNARKYLDQGEIVIFSAGTGQPFFSTDSGAALRAVEVQAEAIIK
                     ATKVDGVYDRDPEKFNNAQFYDRLDYQTAIEKRLKFMDLAAITICQDNDLPIIVYNMK
                     IPGNLRKVLTGEQIGTLITRSQL"
     gene            3759..4313
                     /gene="frr"
                     /locus_tag="PKX09_08255"
     CDS             3759..4313
                     /gene="frr"
                     /locus_tag="PKX09_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOU17853.1"
                     /translation="MDEVVKDAESRMKKSLDTIQQEMAVIRTGRATPALLDPIKVEYY
                     GTTVPLRQVASIGAPDARLLIVQVFDRNAVTSVEKAIRTAELGLNPIVEGNLLRVPIP
                     QLTEERRKELVKYVHKIAEEGRVAIRNIRRDANEMAKELEKDHEISEDELHNGQEEVQ
                     KLTDKYIKLIDELVKRKEKDILED"
     gene            complement(4476..5336)
                     /locus_tag="PKX09_08260"
     CDS             complement(4476..5336)
                     /locus_tag="PKX09_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOU17854.1"
                     /translation="MWYLVALLSGFFMATADAFSKKSAGQTNALLLAWVREAYALPFL
                     LPILFFIEIPELDATFWIAMAICVAMDLVTTLLYMRAIQIAPLSLTVPYLGLTPLFLL
                     IIPTIVLGERLSVIGIIGVILIAIGTYALQIDRAKSGWLEPWLAIFKNRGSLYMLIVA
                     ICYAITATLGKLAIQHSSPFFMAIVYFGLLAIVYTPFLLITTKGKLAPLINTPSNFLK
                     IGLAMALMAITHYSAINMIQVAYMISIKRLSLLFAILYGVFWFHEQNLRERLIGGIII
                     IAGATFIAFA"
     gene            complement(5510..>6798)
                     /locus_tag="PKX09_08265"
     CDS             complement(5510..>6798)
                     /locus_tag="PKX09_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOU17855.1"
                     /translation="INSVKSTLTDITAQSQTTVGNSEKDFDIGNSLAEKGYFLTFILI
                     FLGGLGLNLTPCIYPLIPVTLSYFGGQAVGKKSKTLLLALLYVIGMALINSLLGTLAA
                     LSGSLLGSIMTNPIVLIVIAAILVTLALSMFGLYEFGVPNFLMKLAGGSRSGYLGALF
                     MGLTMGIVAAPCIGPFVIGLLTYVASIGKPFIGFLMFFTLSLGLGLPFIVLAFFAAQI
                     DRLPRSGEWMVGVRQIFGFLLIGMALYFIKPLLPAGLASLILGLYAIASGIYLILFSK
                     SGNNVRTFVFIKNWLAIIAIIAGTWFLKTEKAERIEMQWQTYSLPAFESALEAKRPIL
                     LDFYADWCIPCKELNKYTFSDPEVIHLSQNFALFQIDLTGNVTPEIQTLLEKYAIKGV
                     PTIIFINRDGREIEDLRLLGFEKADSFIRRMHRALE"
     assembly_gap    6799..6898
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6899..7144)
                     /locus_tag="PKX09_08270"
     CDS             complement(<6899..7144)
                     /locus_tag="PKX09_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17856.1"
                     /translation="MKTRRFSVLVIFLICWNFILANESEILSVKVNLLTPNIQADGKF
                     SAEVNVQINENWHINSNKPSDEFLIPTELRIEPSDEYK"
     gene            complement(7314..7955)
                     /locus_tag="PKX09_08275"
     CDS             complement(7314..7955)
                     /locus_tag="PKX09_08275"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012670582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU17857.1"
                     /translation="MKSIVFILVALMASALSAQPLKEADIIAIQKKVQALPDEPVDPN
                     EVVVIETDYGKIVIELFANQAPLHAMNFKKLVKAGYYTGTTFHRVIPDFVIQGGDILS
                     RDANPSNDGTGGPGYTIPAEIGQKHLRGSLAAARLPDQVNPQKASSGSQFYICVKDLP
                     SLDRGGYTVFGRVIEGMNVVDKIVAVPRDQRDRPLKDVVMKSVYITDKKSLKK"
     gene            complement(7981..8772)
                     /locus_tag="PKX09_08280"
     CDS             complement(7981..8772)
                     /locus_tag="PKX09_08280"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU17858.1"
                     /translation="MIFCNLGSGSKGNCTYIRHKQKALLIDQGFSLKNLHARMETTAI
                     DEHSVGAIILTHEHSDHLKGVGVFARYHKVPVYLTEQTLNVINPEFFHKVHVCPFRAG
                     DEVTIEDFKVKTFHIPHDAIDPIGIVISAEQYKIAVVTDLGTVSESVLVQIADPDLLF
                     LEANHDLTMLINGPYPLNLKQRIKSRVGHLSNEQSLMLLQRLAGNGRLKHLILGHLSE
                     INNHPSIVRELFENNINTNLRRYQIEIADQYRPTSIIELTAPHEV"
     gene            complement(8769..9350)
                     /gene="pyrE"
                     /locus_tag="PKX09_08285"
     CDS             complement(8769..9350)
                     /gene="pyrE"
                     /locus_tag="PKX09_08285"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOU17859.1"
                     /translation="MKKDENYYLNILKESGAILSGHFLLTSGLHSATYVEKFKILQYP
                     LLCDKLCRGIAKNFENLSPNVVLGAATGGIIISHSVGRALSTRSIFAERENNELVLRR
                     GFNIANGERVVIVDDVVTTGGSLLELIDLATKYGGYILGIGVLIDRSGGKINFGFPFH
                     SLITLDIPAYKPDKCPQCLANIPLTTRGRTGKL"
     gene            complement(9358..10146)
                     /gene="pyrF"
                     /locus_tag="PKX09_08290"
     CDS             complement(9358..10146)
                     /gene="pyrF"
                     /locus_tag="PKX09_08290"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOU17860.1"
                     /translation="MQFIEKFHECLTEKQTFLCIGIDPDPGKLPSGFAANVDGVYHFV
                     KEIIAATGAYTPAYKFNLAFFEMWGSRGWQMLEKLLSEVPQNILLIGDAKRGDIGNSA
                     KFYAKALFEALPFHAVTLSPYLGTESILPFIADESHGAFVLCVTSNPSGREIQDFGGN
                     QHLYLKVAEIVQQLNQRRNLGLVMGATKPEQLLSVRQKFPNLPFLIPGVGAQGGEIAA
                     AVQVCRTCGVGLINVSRAVLFPDKGKFPENVAETAAYYQEKFKI"
     gene            complement(10221..13076)
                     /locus_tag="PKX09_08295"
     CDS             complement(10221..13076)
                     /locus_tag="PKX09_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17861.1"
                     /translation="MKCISFILNKLTANLKNHIILRISLGLAFSFIFTGFAFARQNGL
                     KYDLDLEFNPSENTLSGHEKIAFTNHAPIALDTIYLFVRANAFRSRDLRSFQKYETQF
                     EAPASINIESITINAKPVSVMQPESVNPAVILAESLQPGDSVELFIQFTTIIPPGKSK
                     LCSTYSEKSYCLIYFYPRIAEYRRGRWQITGFNELERAPLEFANYSVRFTLPDYFQPA
                     GSLQIDSVVSHNDKNTFIFRSEYLQDIGFILNDRLKVSTIRARPHNEVKFISRFSAND
                     KSKDKIATEIINDIIDYYAKYLPEMNQQITIYPTVIPGGFTTSRLILIDKTILAEAVK
                     LDYLSLYILAREIAHQYLGFNVESEYDLSVSISDGLAGFLANDYLKNRYEFLHQKYKL
                     SDNQLISFNTKVFNVFTSGLEKENILQPARASNERANTIFINTQTRFLKSQKLVEMSH
                     YVLGDSLFSQVLAQYQDFIQSDSIDPQVFFKIAEEKSGYEFIALYQNFLHLEQPTDLK
                     IQKVIRSQTLNREHITDVIIKQPSLNYLPLEVNAFDRSGNVYNHRIQPRTGTFDTLTL
                     VTDKPITKIVLDPNRQIWDSNRLNNQYPRSVVFKFLVGLPSIDSYQIFYYPTFDFNNR
                     DIMRFGVKLRGRYWINMRPLFPAQSLDEWSFGLNYGYKSKTFGYDLSYSTSLLAFLFK
                     PRIYLRWRDYFDLMEGKISTEIYLGDVKYWGFNRVQGYQKLNLGLDYQKVRSLEFLNT
                     NKWETGQSLKPYLDYVNFHNWGNIRHIVHAKFVYGLPVGENHFSFDKLTLDGQVKMRL
                     NHRIWLYQRLFYGNAHGNVPKQEYFYLFGKNVLENQSFESYRLAQGEGDMRGYGDQSL
                     KSYKMLTSNTELRRNLALAGAAMFDCIIFLDSGILPESFSAIDWKQTHFDAGLGVELE
                     LLDILKVGMHFPLWVSHPPQSEPRFDLRWVITTDLQL"
     gene            13188..13260
                     /locus_tag="PKX09_08300"
     tRNA            13188..13260
                     /locus_tag="PKX09_08300"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13221..13223,aa:Lys,seq:ctt)
BASE COUNT         4129 a         2642 c         2841 g         3550 t
ORIGIN      
        1 ttagatgatt cagttcgatc ggaatttaaa tcttttaaat tcaagactaa taagaaagat
       61 gtaatgaacg ttagggttaa actgacatta agcacaaata taaaaggtct tgagggaatg
      121 tttattgacg agatcggaac agtcaatgaa aatacattgg ccaaaattgc aattgagcaa
      181 ataatgttag aacaaccaga tgttccggag agttattctc ttgcacaaaa ctttccgaat
      241 ccgttcaatc cgacgaccac gattagctat tccttaccga aggtcgggaa tgtgaccata
      301 accgtgtaca acctacttgg cgaggaagtg gcaacgctag tgaaaggaga gaggcaagaa
      361 ggccgacatt cgattacttt cgatggctcg agactggcaa gtggagaata catcgttagg
      421 atgcagtcag gtaattattc aaaaaccata aaaatattat tgatgaagta atccgcaaat
      481 ttctcattga tatttaaaaa gcccgattta tagtcgggct tttctattct ctaaactaac
      541 gtgagtgaga aatcaagata aaaacattta aaataattta ttacttctct ttaatctttc
      601 tgtccatatc cagattagga ttggcttgtc ctgattgaga aaagatctat tctggttggc
      661 tctgaaagtc gaaagaataa ttaagaatgg atataatgtc tatagatcag acgtagagtc
      721 gtggaaaaat tccttgatta taatatcctc aaatcgtaag tttacccgct atttttgaat
      781 tataattaag agataagcaa tgtacaaaac gtcttttccg aaagtgacag aaattaaaca
      841 agattggtac gtggttgatg ccgaaggtgt agttctcggt cgtttagcca gtaaagtggc
      901 ggcgatttta cgtggtaaaa acaagccgta ttttacgccg catctcgata ccggcgacta
      961 tgtcatagtt attaatgccg ataaagtacg attaactggt cggaaagcag aaattaagac
     1021 ttatttcaag cattctggtt atctcggaca tgaacgcttt ctcaagtata aagatgtgtt
     1081 tcaagccagt ccagaggaga tcatcgaacg agcggttaaa ggaatgttac cgcacaatgc
     1141 gcttggtcgc cagcttttcc ggaagctgaa agtctatcgc ggtccagtcc atcctcatca
     1201 agctcagcaa cctaaacctt tagtaataga aggataagta acggatgccc aaattagaat
     1261 atgtgtcatt aggtcgtcgt aaaagttcaa ttgcccgtct tcgaatgcag accggcgaag
     1321 gcaaagttat tatcaattcc cgtccactgg atgaatattt cggtagggaa tctcttaagc
     1381 gtatgatcat tcagccattc gaagtctgtg gcctaactgg tcagtttgac gttaatgtca
     1441 aagtagaggg aggtggatat accggccagg ctggtgcgat cagattagca atcgctcggg
     1501 ctttggaaca atatgacccg gagctgagag ccaagttaaa accggctggc tttttaaccc
     1561 gtgactcgcg ggtgaaagaa agaaaaaagt atggattgcg cggcgcccgt cgagctttcc
     1621 aattctccaa acgttaaagg cagagagatc agatttatgg taaatgtaac tgtagaaatg
     1681 ttaatgaaat caggtgctca ttttggtcat ttaactagaa aatggaatcc caaaatgaaa
     1741 gattacgtct gtatgacgca aaacggcatc catatcattg atttgaacaa aacgctcgcc
     1801 tgtcttgatg aagcgacgcg ctttattcgg gaagtagtac gcaaaggtgg cgatgtatta
     1861 ttcgtcggaa caaagaagca agccaaagaa attgtccaga gggaagccga tcgctgtggt
     1921 atgttctatg tcgttgagcg ctggttaggg ggaaccttga ccaatttttc aactatcaaa
     1981 cgcagtatcc ggcatttgca aaaattagaa aaagataaat ctattggcta tggtgagaat
     2041 ctgaccaaga aagaaaaatt agcgctcgaa cgtgaacgaa taaaattagc cgatctgcat
     2101 cgcggtatca aagatatgcg taaattaccc gacgtgctcg tggtagtgga tacgacgtat
     2161 gataatatcg ctgtccagga agcccgccgt ttggacattc cgattatagc catcctagac
     2221 accaatgctg atcctaccat tgtggattac ccgattccag ccaatgatga ttcaatccaa
     2281 actattaatt taataattac ggaactggct gatgctgtta ttgaagccaa gaattcacga
     2341 ttggttgaag taccgccacc ggttgaagaa gcagaaagct aaatagctat acagaaagga
     2401 aaaatatggg aatcactgcc acccaggtta aggagttacg agaccgtaca ggcttgggaa
     2461 tgatggaatg caaaaatgca ttggttgaat cagacggcaa tattgataaa gctgtcgaaa
     2521 ttctacgaaa aaaagggatt gccaaagcgg aaaagaaagc ggatcgcgtt gccaaagaag
     2581 gagtaattct ttcctatatt catcccggca ataaactggg agtcttaatt gaagtcaatt
     2641 gcgaaactga tttcgttgcc gccaacacag actttatcaa ctttgtgaaa gatatagcta
     2701 tgcacatcgc ggctactaac ccgctatcaa ttcgccggga agaaatagat ccagcaatcc
     2761 tggaaaaaga aaaagaaatt tatcgcgctc aggttcgggc tcagaacaaa ccggaaaaca
     2821 tcgttgagcg cattgttgaa ggtaaagtcg agaaattcta tcaggaaaat tgtctgttgg
     2881 aacagccgtt cgttaaaaat ccccagatta cggttaaaga ttatgttacc agcataattg
     2941 ctaaaaccgg tgagaatatc aatattgcgc gcttcgtccg ctttcagctg ggcgagagtt
     3001 tcgaaaaata aataacctct aacaggttaa caaatggcat ccattcctta caaacgagta
     3061 atcctgaaat tttcaggaga agcgcttgcg ggtgaaaagc aagtaaattt tgatcccgat
     3121 ataattcgcc aaattgccgg ggaaatcaaa tccgccgctg accttggagt tcaaatggga
     3181 atagttttcg gcggaggtaa tatcattcga ggctcgagtg gcgttggcaa gtcgcttgat
     3241 cgcatcaaag ccgattatat gggaatgtta gcaacggtta ttaacgcttt agcaatgcag
     3301 gatgctctcg aaaaattagg attgcgtgta catgtatttt cagccattca gatgatcgaa
     3361 gtcgcggaat taatgataat tagaaatgct cgtaaatacc ttgaccaggg tgagattgtc
     3421 atcttcagtg ctggaacggg acagcctttt ttcagtactg actccggtgc ggctttgcgg
     3481 gcggtggaag tgcaagccga agccattatc aaagccacta aagttgatgg cgtttatgat
     3541 agagatccgg aaaaatttaa caatgcccaa ttctatgatc ggctggatta tcagaccgct
     3601 attgaaaaac gtctgaagtt tatggacttg gccgctatta caatttgtca agacaacgac
     3661 ttgccaatta ttgtctataa tatgaaaatc ccgggtaact tgcgtaaagt tttaaccggc
     3721 gaacaaattg gaacgctaat tacacggagt caattatgat agacgaagtc gtcaaagatg
     3781 ccgaatcccg tatgaagaaa agtttagata cgatccagca agaaatggct gtgattcgca
     3841 ccggacgggc aaccccggct ttacttgatc caatcaaagt cgaatattat gggacaactg
     3901 ttccgttgcg ccaagtagca tccatcggtg ctccggatgc ccgccttttg attgtccaag
     3961 tttttgatcg aaacgctgtg accagtgttg aaaaagctat tcgaaccgct gaacttggtt
     4021 taaatcccat agttgagggc aatttactaa gggtacctat tccgcaatta accgaagaac
     4081 gtcgtaaaga attggtaaaa tacgtacata aaattgctga ggaaggccgg gttgctatcc
     4141 gtaatatacg ccgcgatgcc aatgagatgg caaaggaact agaaaaagat cacgaaattt
     4201 ccgaagatga gttacataac ggacaggaag aagtccagaa actgaccgac aaatacatca
     4261 agctgattga tgaattagtg aaacgcaagg aaaaggacat cctggaagat tagtccgacg
     4321 ccttaaccaa cttcaaaata aaatattata agccaagaag tcatcaatgt ttgataggca
     4381 aacgatttgt cccctgattc gattttatca ttttcaaatt tcatataaat cagaacggta
     4441 ctactatctc tgtcagcctt taccaataaa ttaaattagg caaaagcgat gaatgtcgct
     4501 ccggcaatta tgattattcc tccaatcagg cgttcccgca aattctgttc atggaaccag
     4561 aatacgccat atagaatggc aaatagcagg ctgagtcgtt tgatcgagat catataagca
     4621 acctgaatca tattaattgc tgaatagtga gtaattgcca taagtgccat tgccaagccg
     4681 atttttagaa aattgctcgg agtgtttatt aatggtgcta atttaccctt cgttgtaatg
     4741 agtaggaacg gcgtgtaaac gattgccagc aaaccgaagt aaacgatagc cataaaaaag
     4801 ggtgaggaat gctggatggc aagtttaccg agagtggcgg tgatggcata acaaatcgcc
     4861 acgatcagca tataaagtga gccgcggttt ttgaaaattg ccagccaggg ttccagccag
     4921 ccggacttgg cgcggtcaat ttgcagtgca taggtgccaa ttgcaattaa gataacgcca
     4981 ataatcccga tgaccgaaag ccgttcgccc aatactatcg tcggaatgat aagcagaaaa
     5041 agtggagtca agccaagata aggcacggtc agtgataagg gagcgatctg gatagcgcgc
     5101 atataaagta aagtggtgac cagatccatt gcgacacaaa tcgccatagc aatccaaaag
     5161 gttgcatcta actcggggat ttcaataaaa aacaaaattg gcaaaaggaa tggcaaagca
     5221 taagcttcac gcacccaagc caaaagaaga gcattagttt gaccagccga ttttttggaa
     5281 aaggcatccg cggttgccat aaaaaatccc gaaagtaaag cgacgagata ccacatatta
     5341 tttcctgatt aaagttctga tttcaagtgc aaaatttaat ggaaaggtgc aaactatact
     5401 atgaaatata gttaatgacg aatagatata aataagccta aagcattact tctgttgtga
     5461 cagattatct tattttacaa taagttcgag aaattaatag tgcaaaattt tattcgagag
     5521 cccggtgcat tcggcgaata aagctatccg ccttttcaaa gcccagaagc cgtaaatctt
     5581 ctatctcgcg accgtcgcgg ttgatgaaaa tgatggtggg gacgcctttg atggcatatt
     5641 tctccagcaa agtctgaatt tccggggtga cgttgccggt taagtcaatt tggaataggg
     5701 cgaagttttg gctcagatga atgacttccg gatcgctgaa agtatattta tttagctctt
     5761 tacaaggaat gcaccagtca gcgtagaaat cgagtaagat cggtcgcttc gcctccagag
     5821 ccgattcgaa agctggtagg gaataagtct gccattgcat ctcaatccgc tcggcttttt
     5881 ccgttttaag aaaccaggtg ccggctatga tggcaataat tgccagccaa tttttaatga
     5941 aaacgaacgt cctgacatta ttcccagact tgctaaaaag tattaaataa atgccgctgg
     6001 caatggcata caaccctaat attagcgaag ccaaaccagc tggtaatagc ggtttgatga
     6061 aatagagcgc cataccgatt aataaaaacc cgaaaatctg gcgcacgcca accatccatt
     6121 ctcctgagcg tggtaggcga tcgatctgag ccgcaaaaaa cgccaaaaca atgaaaggca
     6181 aacccaaccc gagtgacaag gtaaaaaaca ttaagaaccc gataaacggt ttaccgatcg
     6241 aagcgacata agttaaaagt ccaattacaa atgggccaat gcagggcgcg gctacgattc
     6301 ccatcgttag tcccataaaa agcgcaccga ggtagccact ccgactgccg cctgccagtt
     6361 tcatcaaaaa gttaggcacc ccgaattcat ataaaccgaa catactgagt gccagagtaa
     6421 ccagaatagc cgcaattacg attaaaacga taggattagt cataatagat cccagcagac
     6481 tgccggaaag tgccgctaaa gttccgagga gagaattaat tagcgccatc cctatgacgt
     6541 atagcaaagc caacagcagg gttttgctct ttttaccgac tgcttgacca ccgaaataac
     6601 tcaatgtgac cggaattaat ggataaatac agggtgtcaa attcaatccc aagccgccca
     6661 ggaatatcag gatgaacgtc aaaaaataac ctttttctgc caacgaattg ccaatatcga
     6721 aatccttctc agaattgcca actgtagttt gcgactgtgc ggtaatatcg gttagcgtgg
     6781 atttgacgga attgatacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt
     6901 tatattcgtc cgaaggctca atgcgcaatt cggtcgggat aagaaattca tcactcggtt
     6961 tattagaatt gatgtgccag ttctcgttaa tttgaacgtt cacttctgcc gaaaattttc
     7021 catcagcctg gatattgggt gtcagtaaat tgactttaac tgataaaatt tccgattcat
     7081 ttgccagaat aaaattccag catattaaga aaatgactaa taccgagaat ctgcgagttt
     7141 tcatggttaa tttttttcta ctattgataa aaaaagattt cagagttata caccaattaa
     7201 aaatggaata aaacgcctcg aaattaaaat ccaggcaaat atttaaatta ttcggaattt
     7261 gttgatcttt aagaatattc aaaagctggt tataagccga caagaaaatc aattcatttt
     7321 ttaagagatt ttttgtcggt tatatagact gatttcatta ccacgtcttt taacggtcgg
     7381 tcgcgctgat cgcgcgggac agccacgatt ttatcaacga cgttcattcc ttcgatcacc
     7441 cgtccgaaca cagtgtaacc gccccgatcc agagagggca aatccttgac gcagatgtag
     7501 aattgactgc cactggaagc tttttgagga ttgacttggt cgggcaaacg cgccgcggct
     7561 aaactcccgc gtagatgctt ttggccgatt tcagccggaa ttgtatagcc cggtccgccg
     7621 gtgccgtcat tacttggatt ggcatcgcga ctgagaatat cgccaccttg aatgacgaaa
     7681 tccggtatga cacggtgaaa agtcgttccg gtataatagc cggcttttac taatttctta
     7741 aagttcatgg cgtggagagg tgcttgatta gcaaacaact cgatcacgat tttgccataa
     7801 tcggtttcaa ttacgacaac ttcgttggga tcaaccggtt cgtcaggtaa agcctgcact
     7861 ttcttctgaa tagcaatgat gtcggcttct ttcagtggct gagctgaaag tgcggacgcc
     7921 attaatgcaa ctaaaataaa aacgatagat ttcataagtc tccctaattt aaaaatgatt
     7981 tcaaacttcg tgaggcgcgg tcaattcgat aatcgaggtt gggcgatatt gatcagcaat
     8041 ctcgatttga taacggcgca ggttagtatt tatattattt tcaaataact ctcttacaat
     8101 actcggatga ttattgattt cactcaaatg cccgagaatc agatgcttta aacgtccatt
     8161 accggctaaa cgttgcagta acattaatga ttgttcatta gataagtgac caactcgact
     8221 cttgatgcgt tgtttaagat taaggggata aggtccgttt atcaacatcg tcagatcatg
     8281 attggcttcc agaaagagta aatccggatc ggcaatttga accaataccg attccgaaac
     8341 tgtaccgaga tcggtcacca ccgcaatttt atactgctcg gctgaaatga caatgccaat
     8401 tggatcaatt gcatcgtgtg ggatatgaaa agttttgact ttaaaatctt caatggttac
     8461 ttcatcacca gctcgaaagg gacaaacgtg gactttatgg aaaaattccg ggttgatgac
     8521 gttcagcgtc tgttcggtga gataaacggg aactttatga tatcgggcaa agacacccac
     8581 gcctttgaga tggtcggaat gttcatgagt caaaataatg gcaccaaccg aatgctcgtc
     8641 aattgctgtc gtttccatac gcgcatgcaa gtttttcagg ctaaagccct gatcaattag
     8701 aagcgccttt tgtttgtgac gaatgtaagt gcagttacct ttactgccac tgccgaggtt
     8761 gcaaaagatc atagtttacc tgttcgtcca cgggtagtca gaggaatatt agccagacat
     8821 tgggggcatt tatccggttt gtaggctgga atatccaatg taatcaagga atgaaacgga
     8881 aaaccgaaat taatttttcc accgctacgg tcaatcaaca caccaattcc taggatgtaa
     8941 cctccatact ttgtggctaa atcaattaat tcaagaagtg aaccgccggt ggtaacgacg
     9001 tcatcaacga ttacgacgcg ctcgccattg gcaatattaa atccacgtcg cagaaccaat
     9061 tcgttatttt ctctttcggc gaaaatactg cgcgtgctca aagcccggcc aacgctgtgg
     9121 ctgataatga tgccgccggt tgcggctccc aagacgacat ttgggctcaa gttttcaaaa
     9181 ttcttggcga tgcccctaca aagcttatcg caaagtaaag gatattgtaa aatcttaaac
     9241 ttctcaacgt aggtggcact atgcaagcca gaggtcagta aaaaatgacc actcagaatg
     9301 gcgccgcttt ccttaagtat atttaaatag taattctcgt cttttttcat ctaaaaccta
     9361 aattttaaat ttttcttgat aataagcggc tgtttcggcg acattctccg gaaatttacc
     9421 tttatctgga aataaaactg cccgagaaac attaatcaag ccaacgccgc aagttcggca
     9481 gacctgaaca gccgccgcga tttcgccacc ttgagcgcca actcccggta tgagaaacgg
     9541 caaattggga aatttttggc gaacgctcag cagttgttcc ggtttagtgg cgcccattac
     9601 tagcccgaga ttccgacgct ggtttaattg ctgaacaatt tcagccactt taagatataa
     9661 atgctgatta ccgccaaaat cctgaatttc ccgaccggag ggattagatg taacgcatag
     9721 aacaaaagcg ccgtgagatt catcggcaat aaacggcaag atgctctcgg taccaagata
     9781 aggactgaga gtaactgcgt ggaaaggtaa tgcctcgaac agggctttag cgtagaattt
     9841 ggcgctgtta ccgatgtcac cgcgtttagc atcgccgatt agcagaatat tttgcggcac
     9901 ctcgctgagc aatttctcaa gcatttgcca gccacgcgaa ccccacattt cgaaaaacgc
     9961 caagttgaat ttataagccg gtgtataagc gcctgtggcg gcgattattt ccttaacaaa
    10021 atgatagacg ccgtccacat tagccgcaaa accggagggc agtttacccg gatcaggatc
    10081 aatcccaatg cataaaaaag tctgcttctc ggtgaggcac tcgtggaatt tctcgataaa
    10141 ttgcataacg caaaaaatta cgtttttttg tgagattaaa caatataaga gaagtgtcga
    10201 atattagcgt tcaatagaaa ttataattga agatcggtgg taattaccca gcgtaaatca
    10261 aaacgaggct ctgattgcgg aggatgcgat acccataacg ggaaatgcat accaactttg
    10321 agtatgtcca acaattccag ctcgacacct aatccggcat cgaagtgagt ctgtttccaa
    10381 tcaatggcac taaatgattc cggtaatata ccgctatcga gaaaaataat acaatcgaac
    10441 atcgccgccc cagcgagggc aagattacga cgcaattcgg tattgctggt gagcatttta
    10501 tagctcttca gagattgatc gccatagccg cgcatatcgc cctcaccctg agctaaccga
    10561 taagactcaa aactctggtt ttcgagaaca tttttaccga acaaataaaa atattcctgc
    10621 ttagggacat tgccatgggc attgccgtag aaaagccgtt gataaagcca gatgcggtga
    10681 tttaagcgca tcttgacttg accatcgaga gttaatttgt caaaactaaa atggttttcg
    10741 ccaaccggta atccatatac gaatttggca tgaacgatgt gtcggatgtt accccaatta
    10801 tggaaattga cataatccag atagggtttt aaactttgtc cggtttccca tttattggta
    10861 tttaagaact ccaggctacg taccttttgg taatctaatc ccaagttcag tttctgatag
    10921 ccttgaactc gattgaatcc ccagtattta acatctccca aatagatttc ggtagaaatt
    10981 ttaccctcca tcaagtcgaa ataatcgcgc cagcgcagat aaatacgtgg cttgaataaa
    11041 aatgccagca aagatgtgct gtaacttaaa tcgtatccga aagtcttgct tttataacca
    11101 taattcaaac cgaaagacca ctcgtctaaa ctttgagccg gaaataaagg ccgcatattg
    11161 atccagtaac gaccacgtag tttaacccca aaacgcataa tgtctctatt attgaaatcg
    11221 aaagtcggat aataaaagat ttgatacgaa tcaatgctcg gcaacccgac aagaaattta
    11281 aaaactaccg aacgaggata ctgattatta aggcgattgg agtcccagat ttgccggttg
    11341 ggatcaagca caattttagt aatcggttta tctgtaacca aagttagagt atcgaatgtt
    11401 ccagttctcg gctgtattct atgattgtaa acatttccgc tgcggtcaaa ggcatttact
    11461 tcaagcggaa gataatttaa cgacggttgt ttgatgatca catcggttat atgctcgcga
    11521 ttaagcgttt gtgaccgaat gactttttga atttttaaat ccgtcggttg ttctaaatgc
    11581 agaaaatttt ggtagagagc aatgaattca taaccggatt tttcctcagc tattttaaaa
    11641 aatacttgtg gatcaatact atcggattga ataaaatcct gatattgagc cagaacctgg
    11701 ctgaaaaggc tgtcaccgag cacataatgg ctcatttcaa ctaatttttg actttttaaa
    11761 aaacgggttt gagtgttgat aaatatagta tttgcccttt cgttggaagc ccgcgccggt
    11821 tggagaatat tttctttttc caacccagat gtaaaaacat taaagacctt ggtattaaat
    11881 gaaattaatt ggttatcaga cagtttatat ttctgatgca gaaattcgta gcgatttttt
    11941 aggtaatcgt ttgccagaaa tccagccaga ccatcactaa tcgagacact aagatcatat
    12001 tcggattcca cattaaaacc caggtattga tgagcaattt ctcgagctaa tatatacaaa
    12061 cttaagtaat cgagtttaac cgcctcggcc aagattgttt tatcaattaa tatcagacga
    12121 gaagttgtga aaccgccagg gatgaccgtc ggataaatcg ttatttgctg attcatttcg
    12181 ggcaaatatt tggcgtaata gtctattatg tcatttatta tctcagtagc gatcttatcc
    12241 ttggatttat cgttggctga aaaccggcta ataaatttta cttcgttatg tggtcgagcg
    12301 cgaatagtcg aaaccttcag tctatcattg agaatgaatc caatgtcctg aagatactcg
    12361 gagcgaaata taaaagtatt tttatcgtta tgactaacaa ctgaatcaat ttgaagtgaa
    12421 ccagccggct ggaaataatc cggcagggtg aatctaacag aataattggc aaattcgagg
    12481 ggtgcgcgct ccagctcatt aaacccggta atctgccatc tacctcgacg atattctgca
    12541 attcgggggt aaaagtaaat caggcaatac gatttttccg aataagttga acagagtttg
    12601 cttttcccgg gtggtattat agtcgtgaac tgaatgaata attcgaccga gtcgccgggt
    12661 tgaagagatt cagctaaaat tacagccgga ttgaccgatt ccggttgcat taccgaaacc
    12721 ggtttggcat ttatcgtaat tgattcaatg ttgatagatg ccggtgcttc aaattgagtc
    12781 tcatatttct gaaaactgcg taaatcgcga ctgcgaaagg cattggcgcg aacaaacaaa
    12841 taaattgtat ccagcgcaat tggagcgtga ttagtgaaag caatcttctc gtgtcccgat
    12901 aaagtgtttt cactgggatt gaattctaaa tcgagatcat acttcaatcc attttgccgg
    12961 gcaaaggcaa agccggtaaa tataaatgaa aatgctaatc ccagagaaat tcttaagata
    13021 atatgatttt ttaagttagc ggtcaattta ttcaatataa aagaaataca tttcattagg
    13081 aacttaactt aaaattaagt caggatacaa tcaagcttag cgaatcgaac gatgcctgct
    13141 attttgtttg attctccgga aaatcttaaa ttaacccgct tttttaagcc ccaatagctc
    13201 aaatggtaga gcagcggact cttaatccgt tggttcaagg ttcgagtcct tgttggggta
    13261 ca
//
LOCUS       DAOSMP010000052        13148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_4743_length_13148_cov_12.259757, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000052 DAOSMP010000000
VERSION     DAOSMP010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 13148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13148
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4743_length_13148_cov_12.259757"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1625)
                     /locus_tag="PKX09_08305"
     CDS             complement(<1..1625)
                     /locus_tag="PKX09_08305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU17862.1"
                     /translation="MSHKKLAYLNLFLVSATVICFEIIATRISSVIFVSNYAFIILSL
                     AIFGLAMGGVFSYYRFNSSKIPRVIRTIACFIFLTGISLLLFIISVISFSITNPFIYF
                     LFLILPFFFAGVVYALIFKIFADESFKLYASDLIGAAVGSGIALLIFNIFNAPNAVLF
                     LSLIMFLSAAIFAHEFFKKKVAIGIYLLTFLGMIILSCFGHKEFLGEVPIGKFPEKDF
                     YYVYPNLTPFDYKIIDSRWSLYGRSDLVEYRQQDMVKQLFIDGAAGSPMYRFNGNLQN
                     PDVLLLNLLINQSTTIPFMFLQGNERDNMLVIGPGGGKEVLIGLLTGIRQITGVEVNP
                     DFVDIVKDQSFFNGGIYTGFPNVRILVKEGRHFIKSTNQKYDLIVMALPSTEQLQNID
                     HFAASENYLLTVEAIQDYLKILTPEGSLIFTVHNRWELIRLIVNTLYAMEDIGISNQE
                     ALNHFIILAEDYAPTIVIKKNTYRPDDIAVINNIIEQFPPELPHITYRPYHWNQINNT
                     IENQLLRNIRDNRISLQQYIEQDEFDISPVYDD"
     gene            complement(1732..3516)
                     /locus_tag="PKX09_08310"
     CDS             complement(1732..3516)
                     /locus_tag="PKX09_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU17863.1"
                     /translation="MKSNFYLLILLISMTANIFTNCAKKTELDQKTVAVIDDHYKISF
                     AELHKYVYDEFFHRRYRDKSEAYRQALNEMVLDQLKCIDFFKRGLHNDPQLTQELQRY
                     INEELIVKYFESEYLGKYTSEEFARKTYDQMGKEVYYQQIVLNKPENASDAQLDALKN
                     QALEIKTKAEQGVDFKELIKQYAPDNASDVSILTIDWKKSMVSPHNRIIFNLNKGDVR
                     ILDEYNAYYIVKITDVKPIKLKPFDKVKDELITQLQDSYYHTSLEEYDRDKEALINED
                     SIVWNEKALDQLVKWSRIPNFYKAIYRDTLQNAISSGRNLVILTYPEGQVDLKEYLRL
                     LNEILILGDSREITKEQLKEFIVEALRSDKIVRKAKDLGLEKEVFNPYTKNPVLKYKI
                     VELYDKTVIESQIPEPTEAALWEFYQANQDSLYYQLEKINLFAMIFSDKAEADQMWAK
                     IQAGTPFEKASNRWFVKTFIKDRDGQIKSYLSKEPPYLGEAAFKLNEGEVDGVIEYTD
                     PQKGRQYAVIKCEKRTPERQLTYDDVRKSIADDFINYHRKQISQEVTQYLYDNYEVTI
                     NEKVLAKAIKSLSDEKKNSSKSSSKKKK"
     gene            complement(3746..5998)
                     /locus_tag="PKX09_08315"
     CDS             complement(3746..5998)
                     /locus_tag="PKX09_08315"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOU17864.1"
                     /translation="MKKGLLLSVVLGLFLTTAVFAQNNLMDFVSEVRGDTLVIKDYFD
                     MPDEAGSLKSAIDADSVDVPAGRVYELKAGGYYPLSTNLTTPATRAVVIVGADNRRLV
                     NNDDAVSAPPIICGFGSNTGGINFQNDLTVKNCSIIQAANRTTLGWAFFGAAASNKTI
                     RLDNCLFEHTRWVMVQSNDAEGTSVYFTDCYFVNMTGEACRRNGGVYDNVNHNTNTIW
                     AENCTHVMAQGMLYKMRNYPIGKAWFNHNTFINCSGQVFETQGYQSDMVVVNNIFVNS
                     NVQPYMEGLDADETDLDNQPMGIINVRPLPDTYEQVERKVLADRNVIFWDSRLDDMVQ
                     FCIDNQVNDTTAWHSQMITMNARTQAMFDDDETYPYLVEGAWLRKCPTFTDPQDLLTD
                     QVDALREFSVSTVDEASGALLPIWRKVNTNLDDDFLYSDWPIPVDLSYTDKDLLTAAY
                     GRFPVGDLNWFPDKKAAWAAQREAEITAIEELRNSGKIDIATNGGFEQTPAGSVTNLA
                     KGIAGWVIEVAAGIDPAPAFEIVTDPVQEGDHALAITVNALGTNAWDIQVVAEDIPVK
                     PGATYTYSIWAKASAAAKANFTVGNADYSEYMRVHEKALTTEWTEIRKDFTVSDQYTV
                     IRAPIHVNIAGNEGITIYIDNLTITEKPSAIAPKTTPEKFALRQNYPNPFNPTTTINF
                     TLPEPGNVTLKVYNMLGQEVATLINNEHRGANATHSILFDGSKLSSGVYFYTLTYGDK
                     TVSKKMVLMK"
     gene            complement(6140..9277)
                     /locus_tag="PKX09_08320"
     CDS             complement(6140..9277)
                     /locus_tag="PKX09_08320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU17865.1"
                     /translation="MKNAETFKKKETYLLKSRRTNYRIYNGMLFKVGIITLMTVQLVM
                     AAGTVKGRVFDKKSKEALPAANILVKGTNIGTAADLDGNYFLNYVPVGQQVLVVTYIG
                     YEPVEVEVIVPEDGVVIQDLMLSPVAIKGEAVVITGQAASQGAAINQQISSNTITNIV
                     SSSKIRELPDESAAAALSRLPGVSLMEGDKVAIRGVQAKQNLVLVNGVQLPSTDLEDR
                     STNLGFFSSNMLSGIEVTKVLTPDMDANAIGGVVNLRLREAPTGFQSDVFAQGSYNTQ
                     DRTYPGQNFKFWASASNRFFNDKLGVFIQGNAERSDVGNDITSAGYTRDAGTEPYGKG
                     IYQLKDFAFIDEENIITNYGGSIFLDYKLPNGKIILQNALTHTLNDNAKYRLEYDLSI
                     NEIAYALNRDKHNKELLINALQVNYNFGPVYTELGLAHSFSDKNTDIRYGDPGNEFGF
                     QNVKSDVPFVDDNGNPVYYDVDRRFFTPEDVYNIKIKEDDCENAEIYGWFVTRDEAFK
                     QHLYNGTLDFTVPVSFLNIISGDIKLGGKYTLSTRTNDVEESYRRTGAADFYIGIDST
                     WLGEQFNPASNPITFANLRNDDYESKRGKYFFKGDYPFQYALDKDLTDEFVENLIYGW
                     NHPWEFDPTVTKPINYRHKAKSLRDDFDGTEVFSAVYLMGDLNIGPRLSLIGGGRFEH
                     FNMNYDANFVYVKHSVDGDANDTTRSVDRTDDTFFPNAQLRYKFTEWGDIRAAYSKSI
                     SRPDFLAILPNVYYPKSDDALAGNPGLRPSIATNYDVYLSLYSNKIGLFSLGGFYKEI
                     DDVFFRTQVYYPHLSYYGVDFPDSAEMVNAGLNILPAALPGPSNKVTTYVNNPNPAYI
                     KGLELEWQTYFWYLPKPFNVLVLSANYTRTWSDMDYLQIETKDTTISYIDPVTGRQKS
                     RKEYRTVENIRENTRLVGQADHLVNVALGVNYKGFAGRISFRLTDNMLSYVGIRPEED
                     EFTRTVTRWDISLKQKLPIKGLSIGFEGINIFHSPTETYKVFRRSNVTPIIENTATTQ
                     YEPRKFSLYLRYNFE"
     gene            complement(9397..11286)
                     /locus_tag="PKX09_08325"
     CDS             complement(9397..11286)
                     /locus_tag="PKX09_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008101047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOU17866.1"
                     /translation="MRMRFTPTNGNSLPRTNKRFAIGSLFIYLSSFLIAPAQPSGGPY
                     GPIHQTYELPSVKGKIYYVAPDGKAESSGEALDKPTTLENAIEKVKTGDAIILRGGVY
                     RTGGLLLNQGITMQPYKDEKPILKGTYIATDWKNLGNGLWKTTWTRLFPSQPDDWWVR
                     DREGKKTPLHRFNNDMVFVDGKFLQSVGWEGEVDENSYYINYDKGEIYIGIDPTNRLV
                     EITAFNSAIVRTTGEVHSKKSDGKGPTIRGITFTQYAYRAIEIEGKYPEGLSSEADHG
                     KDVVGTTIENCTISYCSRVAGYFKGDHMTFRHCKISDTSTEGLYIIASNDVLLEKNIF
                     TRNNIENITGYFPAAVKIFNQCYRVTCNDNLVIDLPNSNGIWYDVGNVDGVFINNWVE
                     GVGRNDGYFSKDRPWPSENGFFFEISKGVTCAGNVFVNCDHGIWILNSSNAHIYNNTL
                     VNSTACFGRNERSAEGDHFGWHPSTGPDVDEREGHIFVNNLMYGDKDFERPLLFVWQP
                     ASLCERLSQPMLTQLDYNIYVRADERKYPLILWSPAANPNCQIEIKSLAEIRQLYPKF
                     SANSQEFLNYCNPLFKCPELHNFQVIPEFSAVKLGFKLPADVKKLLKSSEKVISYIGA
                     YPPLP"
     gene            complement(11364..13079)
                     /locus_tag="PKX09_08330"
     CDS             complement(11364..13079)
                     /locus_tag="PKX09_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOU17867.1"
                     /translation="MKSFYSNHNKVKSTILLSIMVLSFRLVNAQSSTIQTDVPALKEV
                     YAGDFYLGCLLSYAHIGFANDPYVPGQSDVVDPNGGYLIKYHMNSMSPGNWMKSVNIV
                     DISGSATAYNNASTQFEKDSIDVHPRVKFNGNIIAQLNWAKRQGFTFRGHTLVWHNQH
                     PGTAFFRTGYNSNGERLSKEKMTERMDNYIKEVIRIIHEGWPGLLSAMDVVNEAVKDD
                     GTDRTESEWYITFGDSSYIMKAFEFTRKWTEYYGETQMKLYYNDYNTHIPAKADGIVR
                     ICKPIYEAGYLDGIGMQDHDQYNSPTAEQWITSYEKFEPICDEISVTELDVKPSTDSA
                     TRWATQANQYAALIKCFVERSYFSGRGKIISVSKDGLNDKWAFVTNASLWDAQNQCKP
                     AFYEVVKVGINYNALDSLISITDTLSENKYTIESWELLMTSLSKAQYAMNRNYSYTVS
                     AGDSLENAKNNLDLAIRSLVVSSNNVNEYEIPIPEQFILSQNYPNPFNPATTIGYQLP
                     ISGYSTLKVYNLLGQEVATIFDGIQSAGNYSVTFDASGLTGGIYLYQLKAGNFVENKK
                     FILLK"
BASE COUNT         3892 a         2762 c         2442 g         4052 t
ORIGIN      
        1 ctatcgtcat aaaccggaga aatatcaaat tcatcctgct cgatatattg ttgtagagaa
       61 atccgattat ccctgatatt tctcaataat tgattctcaa tagtattatt gatctgattc
      121 caatgataag gtcgataggt gatatgcggc agttccggcg gaaattgttc gataatatta
      181 ttaatgacag caatatcatc cggtctatag gtatttttct tgataactat cgtcggcgca
      241 taatcctccg ccaggataat gaagtgattc agcgcctctt gattgctaat tccaatatct
      301 tccatagcat aaagcgtgtt cacgataagc cggatcaatt cccaacggtt atgcacggta
      361 aaaatcagac tgccctcggg agttaatatt ttcagataat cttgaatagc ttcgaccgtc
      421 agaagataat tctcactagc ggcaaagtga tcaatattct gcagctgttc ggttgacggc
      481 aaagccatca caattaagtc atatttttga ttggtgcttt tgatgaaatg acgaccttcc
      541 ttaactaaaa ttcgaacatt ggggaaacca gtgtaaattc caccgttaaa aaaactctgg
      601 tccttcacaa tatcaacgaa gtccgggttc acttccaccc cggtaatctg cctgattccc
      661 gtcaacaaac caattaatac ttccttacca ccgccgggac caattaccag catattatcc
      721 ctctcgttcc cctggagaaa cataaaggga atcgtggttg actgattgat taaaaggttc
      781 aacagcaaaa catccggatt ctggagatta ccattaaaac gatacatcgg agaaccagcc
      841 gcaccgtcaa taaataactg cttgaccata tcttgctggc ggtactccac cagatccgaa
      901 cgaccataca aactccaccg gctatctatg attttataat caaatggagt gagattagga
      961 tagacataat aaaaatcctt ctcggggaac tttccgatcg gcacttcccc taaaaattcc
     1021 ttatgcccaa aacagcttag gataatcatc cctaaaaagg ttagtaaata aatgccgata
     1081 gccactttct ttttgaaaaa ttcgtgggca aaaatagcgg ctgacaaaaa cataataaga
     1141 gacagaaata aaactgcatt aggcgcatta aaaatattaa aaattagaag tgctataccc
     1201 gaacccactg cggctccaat caggtcggat gcataaagct tgaaactctc atcggcgaat
     1261 attttaaaaa ttagggcata aacgaccccg gcaaagaaaa aaggcaaaat aagaaacagg
     1321 aaataaataa aaggattagt gatggagaag ctgataacgc tgattatgaa tagaagtaag
     1381 gaaatccccg taagaaaaat gaagcaggcg atcgttcgga ttacccgggg aattttagag
     1441 gaattgaatc tatagtatga aaaaacgccg cccattgcta atccgaaaat ggcaagcgat
     1501 aaaattataa aagcataatt actaacaaaa atcactgatg atattcgggt agcaataatc
     1561 tcaaaacaaa taaccgtagc cgaaacgaga aataaattaa gatacgctaa tttcttatgg
     1621 ctcataaatc aggctaaccc acttcggaat ctaaatacat accccaatta aatcaagtaa
     1681 ctgaaaaaaa atattttcac gctttatttt gtattatttt gcaaactctt ctcatttttt
     1741 cttttttgaa gaagatttag aggaattttt cttttcatca ctcagcgatt taatcgcttt
     1801 ggctaatact ttttcattaa tagttacttc gtaattatca tagagatatt gcgttacttc
     1861 ctggctaatc tgtttgcgat ggtaattgat aaagtcatca gcgatggatt tacgaacatc
     1921 atcgtaggta agttgtcttt ccggcgttct tttttcgcat ttaattaccg catattggcg
     1981 acctttttgg gggtcggtgt attcgattac accgtcaact tcgccttcat ttaatttaaa
     2041 ggcggcttcc cctaaatagg gtggttcctt gctcaaatag gattttatct gcccatcccg
     2101 atctttaata aaagttttaa caaaccagcg attactggct ttttcaaaag gtgtcccggc
     2161 ttgaattttc gcccacattt ggtcggcttc ggctttatcg gaaaaaatca tcgcaaagag
     2221 attgattttt tctagctgat aatataaaga gtcctgattg gcttgataaa attcccaaag
     2281 ggcggcttcg gtcggttcag gaatttgaga ttcaattacg gttttatcat acaattcaac
     2341 aattttatac ttcagaacag gatttttagt atagggatta aatacttctt tttccagccc
     2401 taaatccttg gcttttcgca caattttgtc actgcgcagg gcttctacaa taaattcctt
     2461 taactgttct ttagttattt cacggctatc gcctaaaatt aatatttcgt ttaaaagtcg
     2521 cagatattcc ttgaggtcca cctgaccttc cggataagtt aagatcacta aatttctacc
     2581 gctcgaaatc gcattttgta aggtatcccg ataaatagcc ttgtagaagt tgggaattct
     2641 cgaccacttg accaactgat caagtgcttt ttcattccat actatagaat cctcattgat
     2701 tagtgcttct ttgtctcgat catattcttc caagctggta tggtagtaac tgtcttgtag
     2761 ttgagtgatt aactcgtctt taactttatc aaaaggcttc agttttattg gttttacatc
     2821 ggtaatctta actatataat aagcattgta ttcgtccagt attcggacat cacccttatt
     2881 aagattaaaa ataatgcgat tatgaggact taccatactc tttttccagt ctatggtaag
     2941 gatagaaaca tccgaagcgt tatctggagc atattgctta attaattcct taaaatccac
     3001 accttgttcg gctttagttt ttatttctaa agcctggttc ttcaaagcat ccaattgagc
     3061 atcggacgcg ttttccggtt tatttaaaac tatctgctga tagtaaacct ctttgcccat
     3121 ctgatcatag gttttccgag caaactcttc actggtgtat ttacccagat attccgattc
     3181 gaaatatttg acgattaatt cttcattaat atagcgctga agttcttgcg ttaattgcgg
     3241 atcattatgc aaacctcttt taaaaaagtc aatacacttc agctgatcca ataccatttc
     3301 gtttagcgct tgcctgtagg cttcggactt atcgcgatat cttctatgaa aaaattcatc
     3361 atagacgtat ttatgcagtt cggcgaaact gattttataa tggtcgtcaa ttaccgctac
     3421 ggtcttctga tccagttcag tttttttcgc acaattggtg aaaatattag ccgtcatcga
     3481 aataagcaga atcaataagt aaaaatttga tttcatcata aacctcattt aattggaaga
     3541 ttttctggat taaaatttaa caaactgact ggtaaattca cgacaattaa cgggcgagta
     3601 gtaataatta gttccataaa aataggagtt tttatattct ttatgtcact aatgaatttg
     3661 agtaccattg tttttttatg caaaaccccg gcaaaatata ccggggtttt gcacaagtca
     3721 ttcattaatt gaataataga aatgcctact tcattaatac catctttttg gatactgttt
     3781 tgtcaccata ggtaagggta tagaaataga ctccgctgga aagtttggaa ccatcaaata
     3841 aaatactatg agttgcgtta gctccgcggt gttcattatt aatcaatgtg gcaacttcct
     3901 gacccaacat attataaact ttcagagtaa cattgcccgg ttccggaaga gtgaagttta
     3961 tagtcgtcgt cgggttaaat gggttcggat agttctgacg taaagcgaat ttttccggag
     4021 tcgttttcgg cgcaatagca gaaggttttt cggtgatggt caggttatca atgtaaatag
     4081 taatgccttc atttccggca atattgacat ggataggtgc cctgattacg gtgtactgat
     4141 cgctgacggt aaaatctttg cgaatttccg tccattctgt agttagagct ttttcatgaa
     4201 ctctcatata ttcactgtaa tcagcgttac caactgtgaa attagcctta gccgcggctg
     4261 aggctttcgc ccatattgaa taggtataag ttgcaccagg ttttaccgga atatcctcgg
     4321 caactacctg aatatcccag gcattagttc cgagggcatt gacggtaata gcgagagcat
     4381 gatcaccttc ctgtactggg tcggtaacaa tctcaaaagc cggtgccgga tcaataccag
     4441 cggctacttc aataacccag ccggcaattc ctttggctaa attggtcaca gaaccagccg
     4501 gtgtctgctc aaaaccacca ttagtggcga tgtcaatctt accgctattt cttaattctt
     4561 cgatagcagt aatttcagcc tctcgttgag ctgcccacgc ggctttttta tccgggaacc
     4621 agttcaagtc accaactgga aatcggccat aagcggctgt caagaggtct ttatcggtat
     4681 aagaaagatc aactggaatt ggccaatcgg aatagagaaa atcatcatca agattcgtat
     4741 tcacttttct ccagatgggt agaagagcac cactggcttc atctactgta ctaacactaa
     4801 attcccttaa cgcatcaacc tgatctgtta gcaaatcttg cggatcggta aaagtgggac
     4861 attttcttaa ccaggcgcct tcgaccaggt aaggataagt ttcatcatcg tcaaacatcg
     4921 cctgagttct ggcgttcatt gtgatcatct gagaatgcca ggcagttgtg tcattgactt
     4981 gattgtcaat gcagaattgg accatatcat caagcctact atcccagaaa ataacattac
     5041 gatcagctaa aacttttctc tctacctgtt cgtaagtatc aggtaatggt ctaacgttga
     5101 ttatacccat tggctgatta tccagatcag tttcgtcggc atccaaacct tccatatagg
     5161 gttggacatt actgtttaca aaaatgttat ttacgacaac catatcactc tggtaccctt
     5221 gggtctcaaa tacttgccct gagcaattaa tgaaggtatt atgattaaac caggctttgc
     5281 ctatgggata attcctcatt ttatacaaca taccttgagc cataacatga gtgcagtttt
     5341 ctgcccagat agtattggta ttatgattga cgttatcata aacaccgcca ttacgccgac
     5401 aggcttcacc agtcatattg acaaaataac aatcggtgaa atagacactg gtaccttccg
     5461 catcattaga ttgcaccata acccatctgg tatgttcaaa caaacaattg tccaatcgga
     5521 tagtcttatt cgatgccgcc gcgccaaaga acgcccaacc aagggtggtt ctattcgccg
     5581 cttgaataat actgcaattt ttcaccgtca aatcattctg gaaattaatg ccccctgtat
     5641 tggaaccaaa tccgcagatt attggtggtg cagatacagc atcatcatta tttaccagac
     5701 gtctattatc ggctccgact attaccactg ctctggtggc tggcgttgta aggttggtag
     5761 aaagcgggta atatccacca gctttcaatt cataaacgcg gccggctggt acatctacag
     5821 aatcggcatc aatagcactc tttagagaac cagcctcatc aggcatatca aaataatctt
     5881 taatgaccag agtatctcca cgtacctccg atacaaaatc catcagatta ttctgagcaa
     5941 aaacagcagt tgttaagaac agacctaaca ctaccgataa taacaaaccc tttttcataa
     6001 caaagctcct ttcaataaat ttaacattaa caaccatgaa acaaacgagc tgtagataaa
     6061 tacagctatt aggaaaatcc tctcaccatc cagcctcctt tcttttaatt ggtttattaa
     6121 aggaaaataa atgctataat tattcaaaat tatacctcag ataaaggcta aatttcctag
     6181 gttcatattg agttgtagcg gtattttcaa taatcggagt gacattactt ctccgaaaaa
     6241 ccttatacgt ttcagtggga gaatgaaaaa tgttaatgcc ttcaaatcca attgatagac
     6301 ccttaatcgg taatttctgt tttaaagata tatcccatct agttacggtt ctggtgaact
     6361 catcttcttc aggtctgatt ccaacatagg aaagcatatt atcagttaat ctaaaggaaa
     6421 tacgccctgc gaaaccttta tagttgactc ccaaggcaac attcaccaga tgatcagcct
     6481 gaccgacaag cctggtgttc tcacgtatat tttcgacagt tcgatattct ttcctactct
     6541 tttgccttcc ggttactgga tcaatataag aaatggtagt atctttggtt tcaatttgga
     6601 gataatccat atccgaccag gttcgggtat aattggcact caataccaat acattaaatg
     6661 gcttcggcaa gtaccagaag taggtctgcc attcaagttc taagccttta atataagctg
     6721 gattgggatt attaacataa gtagttactt tatttgaagg accaggtagg gcagctggta
     6781 aaatattcag cccagcgttc accatctctg ctgaatctgg aaaatctaca ccataatagc
     6841 ttaggtgtgg ataatatacc tgtgttctaa aaaatacatc gtcaatctct ttataaaatc
     6901 cacccagact aaataaacca attttattgc tatataacga taaatataca tcatagttgg
     6961 ttgctattga tggtcttagt cctggattac cagccagagc atcatcggat ttcggataat
     7021 atacatttgg taaaatagct aagaaatcag gacgcgaaat tgacttggaa taagccgccc
     7081 ggatgtcacc ccattcggta aatttataac gtagttgagc attcgggaaa aaggtatcgt
     7141 cagttcggtc aaccgatcgg gtggtatcgt tggcatcacc atcaacacta tgttttacat
     7201 aaacaaaatt agcatcataa ttcatattga agtgttcgaa tctaccacca ccaattaatg
     7261 ataagcgcgg tccaatattg agatcaccca ttagatatac ggctgaaaac acttccgtac
     7321 cgtcgaagtc atctctcagt gatttagctt tatgcctata atttataggc ttagttacag
     7381 tcggatcaaa ttcccacgga tggttccagc cataaatgag attttcaaca aattcatcag
     7441 tcaaatcttt atccagagcg tattggaaag gatagtcccc cttgaaaaaa tattttcctc
     7501 ttttactttc ataatcatca ttcctcaaat tggcaaaagt aatcggattg gatgccggat
     7561 tgaattgctc ccctagccaa gtactatcaa ttccgatata aaaatcagcg gcaccagttc
     7621 tacgataaga ttcttcaaca tcattggtcc gtgttgaaag agtatatttt cctcccagtt
     7681 taatatcacc cgagataata ttcaaaaaag agaccggaac ggtaaaatct agggtaccat
     7741 tatataaatg ttgcttaaat gcttcgtctc gagttacaaa ccacccgtaa atttcggcat
     7801 tttcacagtc atcttccttt atttttatat tatagacatc ttccggcgta aaaaaacgcc
     7861 gatcaacatc gtaatagact ggattaccat tatcatcaac gaaggggaca tcagatttga
     7921 cattctggaa accaaactca ttacccgggt cgccatatct tatatcggtg tttttatcac
     7981 tgaaggaatg cgccaaaccc agttcggtat agactggacc gaaattataa ttgacttgta
     8041 gagcattaat aagcaattct ttattatgtt tatcgcgatt tagtgcgtat gcgatttcgt
     8101 tgatactgag atcatactcc agacgatatt ttgcattatc atttagggtg tgggtaagtg
     8161 cattttgcaa tataatttta ccattgggaa gtttatagtc cagaaaaata ctgccgccat
     8221 agttagtaat gatattttct tcatcaataa aagcaaaatc ctttaattgg taaatgccct
     8281 tgccgtacgg ttcggtacca gcatctcggg tatatccggc acttgttata tcattaccga
     8341 catcagaacg ttcggcattt ccctggataa atacacccag cttatcattg aaaaagcggt
     8401 tgctggcact agcccaaaat ttaaaattct ggcctgggta agttcgatcc tgggtattat
     8461 agctaccctg ggcaaataca tcagattgaa atccggtcgg agcttctctt aatctcagat
     8521 tgactactcc accaatggcg ttggcgtcca tatccggagt gagcacttta gtgacttcga
     8581 taccggatag catattagat gagaagaaac ccaaattggt cgaacgatct tccaaatcgg
     8641 tggacggtag ctgtacacca ttaactaaaa ccaaattctg tttcgcctga acgccacgga
     8701 tagcgacttt atcgccttcc atcaatgaga ctcctggtag gcgacttaga gcggccgccg
     8761 cgctttcatc cggcagttcg cgaatttttg aacttgagac aatgttcgtg attgtattcg
     8821 aagatatctg ctggttaatt gcggcgccct ggctggcggc ttggcctgtg attactaccg
     8881 cttcaccttt aatcgcaacc ggtgagagca ttaaatcctg aataactaca ccgtcctctg
     8941 gcactataac ttcaacttca actggttcat aaccgatata ggtgactacc agtacttgtt
     9001 gacccaccgg aacatagtta agaaaataat tgccatccaa atcagccgcc gtcccaatgt
     9061 tggtgccttt gactaaaatg ttagcggctg gtaatgcttc tttgcttttt ttgtcaaaca
     9121 ccctaccctt tacagtacca gctgccatta ccagctgaac tgtcattagg gtaataattc
     9181 caactttgaa aagcatacca ttataaattc ggtaatttgt tcgcctactt ttgagtaaat
     9241 aggtttcctt tttcttaaaa gtttctgcgt ttttcataaa gctactatcc tcttcttatt
     9301 ggtttaatat taattgaatc gtttcaaaat gtcaagaaaa attttaatac acaatgtctg
     9361 atctatggaa atggtatcaa cttttttatt gaaaaattat ggtagaggtg gataagcacc
     9421 tatataacta ataaccttct ctgaactttt taataatttt ttgacatctg ccggtagctt
     9481 aaaacctaat ttaactgctg aaaattctgg tattacttga aaattgtgca attccggaca
     9541 tttaaacagg ggattgcaat aattcagaaa ttcctggcta ttggctgaaa attttgggta
     9601 tagttgccgt atttctgcta atgatttgat ttctatttga caattaggat tagcggccgg
     9661 gctccataaa atcaggggat attttcgttc atcagcccgc acgtaaatat tataatcgag
     9721 ttgagtcagc atcggctggc taagccgttc acacagcgaa gccggttgcc agacaaatag
     9781 caatggtcgt tcaaaatcct tatcaccata cattaaatta ttgacgaaga tatgcccttc
     9841 cctttcatcc acatccggtc cagtgctggg atgccagccg aaatgatcac cttcggcact
     9901 gcgttcatta cgcccgaaac aggcggtact attgactaaa gtattgttgt aaatatgggc
     9961 gttcgaacta ttaagaatcc agattccatg gtcacaattc acaaagacat tgccggcaca
    10021 ggttactcct tttgatattt caaagaagaa gccgttttcg ctcggccagg ggcgatcttt
    10081 ggaaaaatag ccgtcattac gcccgacgcc ttcgacccag ttattaatga atacgccgtc
    10141 aacattgccg acgtcatacc agataccatt tgaattcggg agatcaatca ccagattatc
    10201 attacaagtc acccggtaac attgattgaa aattttgacc gccgctggaa aataaccggt
    10261 gatattttcg atgttattgc gcgtaaaaat gttcttttcg agtagtacat cattggaagc
    10321 aatgatataa agaccttcgg tactagtgtc gctaatttta caatggcgga aggtcatatg
    10381 atcgcccttg aaatatccag ccacacgtga acaataggaa atcgtgcagt tctcaatggt
    10441 agtgccaacg acatccttgc catgatcagc ttcagaggat aaaccttccg gatatttacc
    10501 ttcgatctca attgcccgat aagcatactg agtaaaagtt ataccacgga tagtcggacc
    10561 tttaccgtcg gatttcttgc tgtgtacttc ccctgtcgtt ctgacaatgg cgctgttgaa
    10621 agctgttatt tccaccagac gattggttgg gtctatacca atgtaaattt ctcctttatc
    10681 gtaatttatg tagtagctat tctcgtcaac ttcgccttcc cagccgaccg attggagaaa
    10741 tttgccgtcc acaaaaacca tatcattatt gaaacgatgc aagggagtct ttttgccttc
    10801 gcggtcgcga acccaccaat cgtccggctg ggagggaaac agacgagtcc aggttgtttt
    10861 ccacaatccg ttccctaaat ttttccagtc ggtcgcaatg tatgttccct tcaatattgg
    10921 cttttcgtct ttataaggtt gcatcgtaat accttgattt aggagtaaac cgccggtgcg
    10981 atagacgccg ccgcgtagaa ttatggcgtc cccggttttc accttttcaa tagcgttttc
    11041 caaggtggta ggtttatcaa gtgcttctcc tgacgactcg gcttttccat caggggcaac
    11101 gtaatatatt ttccccttta cactcggtaa ctcataggtt tggtgaatcg gtccatacgg
    11161 accaccggat ggctgagccg gtgctatcag aaatgaactt aagtaaataa acaaactacc
    11221 gatggcgaat cgtttgtttg ttcgcggtaa tgaatttccg tttgtcgggg tgaatcgcat
    11281 tctcattttt aatttccttt cttcagttat caaatatcct aggataaata taccatattt
    11341 tttatgcctt ttataaatac attttatttc aacaagatga attttttatt ctccacaaaa
    11401 ttcccggctt tcaattgata taaataaatc ccgccggtca atccactggc atcaaaggtt
    11461 accgagtaat ttccggctga ttgaatgcca tcgaatatcg tggcgacctc ctgcccaagc
    11521 agattataga ctttgagagt tgaataaccg gatattggca gttggtaacc gatggttgta
    11581 gctggattga aagggttggg ataattctgg ctgaggatga attgctcggg aatcggaatt
    11641 tcatattcgt taacattatt cgaactaacc accaaacttc taatcgctaa atcaagatta
    11701 tttttagcat tttctaaact atctccggca gaaactgtat aggaataatt tctattcata
    11761 gcatattggg cttttgataa tgaagtcatt agtagttccc atgattcaat ggtgtattta
    11821 ttttcactca gcgtatcagt gatagagatg agtgaatcaa gtgcgttgta atttatcccg
    11881 acttttacga cttcatagaa ggctggtttg cattgatttt gggcatccca gagagaagca
    11941 ttggttacaa atgcccattt gtcattcaag ccgtctttgg aaacgctgat gatcttaccg
    12001 cgcccggaaa aataactgcg ttcaacaaaa catttaatta aggcggcgta ttgattagct
    12061 tgggtcgccc agcgggttgc ggaatcggtg ctgggtttga cgtccagctc ggtaactgaa
    12121 atttcatcgc aaatcggttc aaatttttca taggaagtaa tccactgttc agcggtgggt
    12181 gaattgtatt ggtcgtgatc ttgcataccg atcccatcca gatatccggc ttcataaatc
    12241 ggcttgcaaa ttctcacaat accatcagct tttgcaggaa tatgagtatt ataatcatta
    12301 tagtaaagct tcatctgagt ttcaccgtaa tattcagtcc atttacgagt gaattcaaaa
    12361 gccttcatta tataactgct atcaccaaaa gtaatgtacc attcgctctc agttcgatca
    12421 gtgccatcat ctttaacggc ttcatttacc acatccatcg ccgaaagcaa gccgggccat
    12481 ccctcatgaa ttattctgat cacctcttta atataattgt ccatgcgctc agtcattttc
    12541 tcttttgata agcgttctcc attactatta tatccggtac ggaaaaaagc agtcccagga
    12601 tgttgattat gccaaactaa agtgtgtccg cgaaaagtaa atccctgacg ttttgcccaa
    12661 ttaagctggg caataatatt gccattaaat ttcacccttg gatgaacatc tatagaatcc
    12721 ttttcaaact gagtagaagc attgttatag gctgtggcgc ttccggatat atctacaata
    12781 tttactgatt tcatccaatt tcctgggctc atcgaattca tatggtattt aattagatag
    12841 cctccatttg ggtcaacaac atcagattgc cctggcacat agggatcatt ggcaaaacct
    12901 atatgagcat aggatagtag gcagccaagg taaaaatccc cggcatagac ctctttcaaa
    12961 gccggcacat ctgtttggat agtgctagat tgagcattaa ctaatctgaa agatagaacc
    13021 atgatagaca ataaaattgt cgattttact ttattatgat tcgaataaaa tgatttcatt
    13081 tgaaaattcc ttttatgttc ttaatcctac ttcaaaagga ccagtttttt tgtttccaca
    13141 aagccgtt
//
LOCUS       DAOSMP010000053        12756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_4910_length_12756_cov_14.646563, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000053 DAOSMP010000000
VERSION     DAOSMP010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 12756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12756
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4910_length_12756_cov_14.646563"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..1462)
                     /locus_tag="PKX09_08335"
     CDS             complement(194..1462)
                     /locus_tag="PKX09_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU17868.1"
                     /translation="MLEFSPSLVVSDGHGRVFELEQYQALGISGENVVEIPPDEWIPL
                     PEGSQLMELPGRLPVGKDRRSSEVATLTENGAGKLLAVAAFVAPAYTIFYHAAWEKLP
                     RARRLSLFAYSAVGWFDGCFYVPAIRIDPDIRQDPPNFNQAEIARSAQRQLKLFPHNR
                     LVAHLVNNCALTYGCPAALNYLLNRWEMPLPTARTCNSACLGCISLQRNSGVCSAQHR
                     INFTPTVAEIVEIAVPHLDTAPNPVVSFGQGCEGEPLMNPQLLYQAIEGIRQKTARGT
                     INLNSNASRPDVIIKLIEAGLDSLRVSLNSARPQFYTHYFRPRNYTFDAVLESIRLMK
                     NANRFVSINYFTFPGFSDQPSEIKAFEKILADYNIDLIQWRNLNIDPEWYWEELNPPE
                     EDGLGIRQLIEYYQAEFPRLRHGYFNPSLK"
     gene            complement(1527..2618)
                     /locus_tag="PKX09_08340"
     CDS             complement(1527..2618)
                     /locus_tag="PKX09_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU17869.1"
                     /translation="MNKVRVGVVGTSWWADGMYLPALQSHPQAEIVAICGRNRPRATE
                     MAEKYNIPKIYTDYRVMIEQGDLDAIIIGVPDDLHYDIAIRSLEAGLNVLCDKPLALK
                     EQQAYEMWQKAEAAHVQNMVLFTYRWMPFFQYVHDLIKQGYIGQCYHAEFSFLVGHGR
                     KPDYQWRLDSKRSNGALGDLGSHLIYLARWLIGEIAQVDAQLASFAKRLGPDGEPISP
                     ANDSAVLLFEFTNGAQGVIHTSFMTYLADRDMQIYVKLYGSDGSLEVNFPSSGSDFTA
                     SIFGANNSDKKFRKLEIPASYWGDVNPAEPFGIFTRQAVGCRLFIDAILENRPVSPNF
                     FDGYKVQQVIEAAINSDRTGKRVAIDHSR"
     gene            complement(2621..3940)
                     /locus_tag="PKX09_08345"
     CDS             complement(2621..3940)
                     /locus_tag="PKX09_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17870.1"
                     /translation="MEQEKRERIYTFDFAKGVAILLIIPLHALICQIGKNDPAFFQPL
                     ITAIPENVIYLLIPAMILSMWGPIFTLITGANIAYGYLRVYSRNPDDSSAYILRRILS
                     AVMLIFVSRAAVFIFKGGLFTNGFFNIFNYKIRYYADTLDSIALTGIIVPVFILLFLD
                     KSKSIKQKNETGKAVSQKDIKRIYIGMTVITVIWFIFTPVVHALTPYVQDFASKHHLR
                     LLMLVWSKLTAGRFRFFPILGFGYAGAVIGTAIHAKSRYKNIRKYSGIFFLVTLVGFL
                     IWILLKGNPMVNIASDDIPIMMQVLNLGTMTFAMVLVLGYLDYCPPEVRRRRIERTLW
                     IRRFSIVSLTIYVMEFFLAEGVYWVFAQFWDNAVALVNEVPIFIWNFAQIMVFIIVIC
                     LIWGLIVKLWEKVDFIFSLEWFMIKAVALFQRDKHARINSHKILYGQ"
     gene            4278..5243
                     /locus_tag="PKX09_08350"
     CDS             4278..5243
                     /locus_tag="PKX09_08350"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOU17871.1"
                     /translation="MKKLNSLIGSLFITVALFYLTGELHSEPTSADISYLKIIKNGLV
                     REGPASYYKIAENLEIGMIVQAIEKHDDWYLIKTHSGRIGWIVASDVTPQVQSQDLMA
                     KTNLQNGSPTNIDQKVPFLFTENLELLATGFLWTEPDGSGKIISYLIPGLRVTKLETN
                     GIFYKIQLQNGLIGWADQSLFTIAEPKLPLPTSKIVAKNGNLRQEPSLSAPVIEKIPK
                     GKSVAIIDSTDGWYKIKTASDLTGWLHQGLLSGETGLQNGGKLSVKILLRNGNLRQSP
                     NLAGKVIQVIPAQTAITVLDSLSGWYQVHLPDETIGWINQSLFEK"
     assembly_gap    5278..5377
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5394..6506)
                     /locus_tag="PKX09_08355"
     CDS             complement(5394..6506)
                     /locus_tag="PKX09_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOU17872.1"
                     /translation="MDKVDIVIIGAGVVGLAIAHELSAKLQAEIVLLEKHPYFGAETS
                     SRNSEVIHGGMYYPTDSLKARFCVRGNRQLYEICQKYRIPYRKTGKLIVASTAEELAK
                     VEALHQQGNLNGVPNLVMLSEKQIREIEPEVRAKNALYSPETGLIDVHRLMQFYESQA
                     LSNGVLIAYQNTVTGIEKQSNGYRITVSDATGADWDILSQVVINSAGLNADKIAALVG
                     INIDENHYRLHYCKGEYFNVSNRHQGKIQHLIYPVPTDYSLGIHARLRLDGTLSLGPN
                     AIYIDEINYEVDPGHRADFYNSVKDILPYIKYEDLTPEMAGIRAKLQGHGERFRDFVI
                     REESDKGLPGFINLIGIDSPGLTSAPAIAEYVASLL"
     gene            complement(6516..7049)
                     /locus_tag="PKX09_08360"
     CDS             complement(6516..7049)
                     /locus_tag="PKX09_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17873.1"
                     /translation="MLTKIKNALRQPENRRIIAIVILLFIIINVIAWLSLRPYMKQPP
                     CYVCGRAKTRPVKTLYQYRVEVLPYMKSVDIWYCERHYQHAAEIITKLPIKNDSVASR
                     FRIAVIAGTFSLFSLLFIIILLEISFNWLFIHSGLIALTYLIGGVTSNLTVVILLIST
                     FALAIGIFYFWNRWFNK"
     gene            complement(7123..9087)
                     /locus_tag="PKX09_08365"
     CDS             complement(7123..9087)
                     /locus_tag="PKX09_08365"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOU17874.1"
                     /translation="MKRFLLTIIFLISITAFSLAQISSDWIEQEIRTKTVWLENQRAA
                     LIQLPSYANTNAYDVTYYTLDFRFDIASSILYGEVTIRGLAAESNLQRIDLDMDDNLS
                     VTSIGGDAISYTHRNNILSLQLNKILNENETFNVSITYSCLEKKNNDRGIYFQYHNST
                     PVISTLSEPYYAHTWFPCKDRLGDKADSVDINITVPSELKAVSNGTLVRIIENDDGTC
                     TYCWQERYPIAVYLISLAISDYTYWSENYFGIDGFSSMPLEFWIYPDFDTIARPFLNM
                     TADMMTHFASLWGEYPFIKEKYGQVQFSWGGGMEHQTATSLGSFSEMLICHELAHSWW
                     GNDVTCASWKHIWLNEGFARYAEALWREKDEGTIGLKNYMTDLNRPSRWRAGSLYVQD
                     TSDVNNIFNQIVYDKGAWVLHMLRGLTGYEKFFEILKRYRQEYSGGFATTADFQKKCE
                     EIYDQSLDWFFEQWVYGVGQPHYQVNWQAYRHSLNSWKLKVAISQIQTGATIFRMPLT
                     LFFSDSLNDTTFIVTDSLATQYFTFYCNFKPDSIALDPDNWVLKNATYFNFYSEPQTR
                     PEEFRLHPSYPNPFNQWVNIKLDLPYDIPGRLAIYDLTGREVAILQDGLFKAGQYKTQ
                     WQPQGSASGLYFVSFKARYIHLRQKIVYLK"
     repeat_region   9416..12755
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=9416..9451
                     /rpt_unit_seq="gtttcaatccttggtttagtggataaaggacttaga"
BASE COUNT         3678 a         2619 c         2687 g         3672 t
ORIGIN      
        1 ccattttttc cgctgggact tcgcttccca gccggagatg ttcttaatgc cgtaagtaaa
       61 ttgaacggtc agatttttgg ttctcctttt tactaagcct ctttactatt tactcaacct
      121 ctttaccaat gaccaatgac caatgacctt tctccgcctt atttttcgtg ccttggtggt
      181 ggaatagctt tgattacttc aaagagggat tgaaataacc gtgacgtagg cggggaaatt
      241 cagcttgata gtattcgatt aattgccgaa taccgaggcc gtcttcttcc ggcggattaa
      301 gttcctccca gtaccattcc gggtcaatgt tcaagttgcg ccactgaatc agatctatat
      361 tataatcagc tagtattttt tcaaaggctt tgatttccga aggctggtca ctaaaaccag
      421 gaaaagtaaa ataattgata gagacaaaac gattagcgtt tttcattaga cggattgatt
      481 ccagtacggc atcaaatgta taattcctgg gacgaaagta gtgagtgtaa aattgcggtc
      541 gagccgaatt caatgagacc cgcaaggagt ctaaaccagc ttcaattaat tttataatta
      601 catcgggacg actggcattg ctatttagat tgatagtgcc tctggcggtt ttttgccgaa
      661 tgccttcgat agcttgatag agtaattgcg gattcatcag gggctcgccc tcacaaccct
      721 gtccaaagct gaccaccgga ttgggcgcgg tgtcaagatg tggcacggca atttcaacga
      781 tttctgccac ggttggagtg aaattaatgc gatgctgagc actgcaaacg ccagaatttc
      841 gttggagaga aatacaaccc aagcaagcgg agttgcaggt gcgcgccgtt ggtagcggca
      901 tttcccagcg attcaataag taattcaaag ccgctggaca gccgtaagtt aaagcgcaat
      961 tattgacgag atgcgccact aaccggttat ggggaaaaag ttttaactga cgttgggctg
     1021 atctggcaat ttctgcctga ttgaagttcg gcggatcctg acggatgtcc gggtcaatac
     1081 gtattgccgg aacgtaaaag catccatcaa accagccaac agctgagtaa gcaaaaagtg
     1141 aaagtcgccg ggcacgtggc aatttttccc aagcggcgtg ataaaaaata gtgtaagccg
     1201 gcgccacaaa agccgcgacc gccagcaact ttccagcgcc gttttcggtc agagtcgcta
     1261 cttcactact ccgtcggtct ttgccaaccg gcagacgacc gggtaattcc attagttgac
     1321 taccttccgg gagcggaatc cattcatcgg gaggtatttc gacgacgttt tcaccggaaa
     1381 tgcccagcgc ttgatattgc tcaagttcga aaacgcgtcc gtgaccatcg gataccacta
     1441 atgaaggaga aaattcaagc aattgcttac accaccatca ttaattgatt tctttaagcg
     1501 aaaagttacg tccgttaaca ggcaatttac cgcgaatgat caatggctac tctttttccc
     1561 gtacggtcag aattaatcgc ggcttcgata acctgttgga ctttatatcc atcgaaaaaa
     1621 ttgggactga ccggccgatt ttccaatata gcatcaatga acagacgaca ccccaccgct
     1681 tgccgggtaa aaattccaaa aggttccgcc ggattcacat caccccaata cgaggctggt
     1741 atctctaatt tccggaattt tttgtcagag ttattggcgc cgaaaatgct cgccgtaaaa
     1801 tcgctgcccg aactcgggaa attaacttcc agactaccat cggaaccata taacttgacg
     1861 taaatttgca tatcgcgatc agccagataa gtcataaaac tagtgtgaat tactccttgc
     1921 gccccgttgg taaattcaaa caataaaaca gccgagtcat tagctggaga tattggctcg
     1981 ccatctggtc ctaaccgttt agcgaaggaa gccaattgcg catcaacctg ggcaatttca
     2041 ccaatcagcc agcgcgccaa atagatcaaa tgtgatccca gatcgccgag agcgccatta
     2101 gaccgcttgc tatccagtcg ccattgataa tccggcttac ggccgtgacc aactaaaaag
     2161 ctgaactcgg catggtaaca ttgtccaatg tatccttgct taattaaatc atgcacatat
     2221 tgaaaaaacg gcatccagcg gtaagtaaat aaaaccatat tttggacgtg agccgcttcc
     2281 gctttttgcc acatttcgta agcctgttgc tcctttaacg ccagcggttt atcacaaagc
     2341 acgttcaaac cggcttcgag acttcgtatt gcaatatcat agtgcaaatc gtcaggaacg
     2401 ccgatgatta ttgcatccaa atcaccctgt tcgatcatta cccggtaatc agtatatatt
     2461 tttggaatgt tatatttctc agccatttcg gtggcacgcg ggcgattccg gccacagatt
     2521 gcaacaatct ctgcctgcgg atggctttgc aaagccggta ggtacattcc gtccgcccac
     2581 cagctggttc cgacgacgcc gactctgact ttattcatag ttattgacca tatagaattt
     2641 tatgggaatt aatccgggca tgtttgtcac gctgaaaaag agcaaccgct ttaatcataa
     2701 accattccag tgaaaaaata aaatccactt tctcccagag tttgacgatc aaaccccaga
     2761 ttagacaaat gacaattata aataccataa tttgggcgaa attccagata aatattggga
     2821 cttcattcac aagggctacg gcattatccc agaattgggc aaaaacccag taaacaccct
     2881 ccgccagaaa aaattccata acgtaaatcg tcaatgacac aatactaaac cgccgaatcc
     2941 acagagtccg ttcgatccga cgtcggcgaa cttccggtgg acaataatct aaataaccca
     3001 gcaccagaac catcgcaaat gtcattgttc ccagattcaa tacttgcatc ataatcggaa
     3061 tgtcatccga agctatattg accattgggt ttcccttaag caaaatccag atcaaaaatc
     3121 ccacaagcgt tactaaaaag aatataccgg aatatttacg aatattttta tagcgacttt
     3181 tggcatgaat agccgtcccg ataaccgcac cggcatatcc aaaacccagg attgggaaga
     3241 agcgaaaccg ccccgctgtc aatttactcc agaccaacat caataatcgc aaatgatgtt
     3301 tggaggcaaa atcctgcaca tagggcgtca gagcatgcac taccggagta aatataaacc
     3361 aaatcacagt aattaccgtc ataccgatat aaatacgttt gatgtccttt tggctaacgg
     3421 ctttaccggt ttcatttttt tgctttattg acttggactt atccagaaaa agtaaaataa
     3481 ataccggtac gataattccg gtcagcgcaa tcgaatccag cgtatcggca taatagcgaa
     3541 ttttgtaatt gaaaatattg aaaaaaccat tcgtaaacaa accacctttg aaaatgaaca
     3601 cagccgctcg actaacaaaa atcagcatga cggctgaaag aattcgccgc agaatatagg
     3661 ctgaactatc atccggatta cgactatata cacgtaaata gccgtaggcg atattagcgc
     3721 cagttatcag agtaaaaatc gggccccaca ttgataatat catagctggt atcagtaggt
     3781 aaatcacatt ctcagggatg gccgttatta aaggctggaa aaaagccggg tcgttcttac
     3841 cgatttgaca tatcaaggca tgcaatggaa taatcagtag aatggctact cccttagcaa
     3901 aatcgaaggt gtatattctt tcgcgttttt cctgttccat atttaataaa atacaatcac
     3961 tacatatttt ttataatata ctaccgagcc agagatgaac caagaaaatc actagcaaat
     4021 taaacctcat agctgggtca atttactttt gggaacataa tcggctggag aataaatcag
     4081 ctgaatgaat cgccttgtct aattcgctta agttattttt gatgtaggaa agtcttaaca
     4141 actatcaaat gacataaaga tgattcccta caaataatta ctattttatc caattaggag
     4201 caattaaaaa attgcaagtc actttacaga aatcacttga ttttgtaaat tgccacgcga
     4261 ttttgagaat gggcattttg aaaaaattaa atagtcttat tggtagtctg tttataacgg
     4321 tagccttatt ttatctaacg ggtgagctcc attcggaacc cacctcggct gacatttctt
     4381 atttaaaaat aatcaaaaac ggcttggtac gcgaaggccc agcatcctac tataaaattg
     4441 ccgaaaatct ggaaatcggt atgattgttc aagccattga gaaacacgac gattggtatc
     4501 taattaaaac acactccgga cgaatcggct ggattgttgc cagcgatgta acgccccaag
     4561 ttcaatccca agacttaatg gcaaaaacta acttgcaaaa tggtagcccg acgaatattg
     4621 accagaaggt accattcctg tttacggaaa accttgaact tctcgcaact ggctttcttt
     4681 ggaccgaacc ggacggttcg gggaaaataa tcagctattt aattcctgga cttcgggtaa
     4741 caaagctcga aaccaatggt attttttata aaatacaatt acagaatggc ttaattggtt
     4801 gggcagatca gagccttttt accatcgcgg aaccgaaatt accgctaccg acttctaaaa
     4861 tcgtcgctaa aaatggcaat ttaagacaag aaccgagcct gtccgctccg gttattgaga
     4921 agattcctaa aggcaaatct gttgcgatca tagatagcac cgatggttgg tacaaaatca
     4981 aaaccgcgag cgatttaacc ggctggcttc atcagggttt attaagtggg gaaaccgggt
     5041 tgcaaaacgg tggcaaatta tccgtaaaga ttctgctacg aaacggaaat ttacgtcagt
     5101 caccaaacct ggctggcaaa gtcatccaag taataccggc tcaaactgcg ataacggttc
     5161 tggatagcct ttctggctgg tatcaggtgc atcttcccga tgaaactatc ggctggatca
     5221 atcaatcact ctttgagaag taataattca tagtaggaca ggcattcttg cctgtctnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa atggcttatt aatttaaagc
     5401 aagctggcta catattcggc gatggctggt gctgaagtca atcccggtga atcaatgcca
     5461 attaaattga taaaacccgg caatccttta tcactttcct cacgaatcac aaaatcgcga
     5521 aacctttcac catgtccctg caactttgct cgaatcccag ccatttcggg cgtaaggtct
     5581 tcgtatttaa tataaggtaa aatatctttc acgctattat aaaaatctgc tctgtgcccg
     5641 ggatctacct cgtaattaat ctcgtcaatg taaatggcat ttggtccgag tgacagcgtc
     5701 ccgtctaatc gtaggcgagc atgaatgccc aggctataat cagtcggtac tggataaatt
     5761 aggtgctgga ttttcccctg atgccggtta gaaacattga aatattcacc cttgcaataa
     5821 tgcaaacgat aatgattctc gtcaatattg ataccaacta aagccgcgat tttgtctgca
     5881 ttcagcccag ccgaattaat cacaacttga cttaaaatat cccagtctgc acctgttgca
     5941 tcggatactg taatcctgta cccattggac tgtttctcta tcccggttac agtattttgg
     6001 taagcgatca agacgccgtt tgaaagggct tgcgactcat aaaactgcat tagacggtga
     6061 acatcaatta agccggtttc tggactgtat agagcatttt tcgccctcac ttccggttct
     6121 atttcccgaa tctgtttttc gctcaacatc accagattgg gtacaccatt cagattgcct
     6181 tgctgatgga gtgcctcgac ttttgccaat tcttcagcgg tagaagcaac aatcagtttg
     6241 ccggttttgc gataggggat tcggtatttt tgacaaattt cgtagagttg acgattaccg
     6301 cgcacgcaaa accgggcttt gagactgtcc gtcggataat acattccgcc atgaatgacc
     6361 tcgctgttac gcgaagaggt ttctgctccg aaataagggt gtttttccag caacacaatt
     6421 tctgcctgaa gtttggcgga aagttcatgt gcgattgcta atccaaccac accagctccg
     6481 ataattacga tatccacctt gtccatttta gtctattatt tattgaacca acgattccaa
     6541 aaataaaaaa ttccgattgc gagtgcaaag gttgaaatta ataatattac cacagttaag
     6601 ttggaagtca cgcctcctat caagtaagta agcgcaatta atccagaatg gataaaaagc
     6661 cagttaaaac taatttccag taaaatgatt ataaatagta gcgaaaaaag tgaaaaagtt
     6721 ccggcgataa cggcgattct aaaacgacta gccaccgaat cattcttaat cggtaacttg
     6781 gtgataatct cagccgcatg ttgataatgc cgttcgcagt accaaatgtc aactgatttc
     6841 atatagggca aaacctcaac tcggtattga taaagcgttt tgaccggacg ggtctttgcc
     6901 cgtccgcaaa catagcaagg cggctgtttc atataggggc gaagtgatag ccaagctatc
     6961 acatttatga taataaatag taagataaca atggcaataa ttcgccggtt ctccggttgg
     7021 cgcaaagcat ttttaatttt agtcagcatt tatctcaaaa ttctcgttgt ttatattttg
     7081 ggctgaattt ataaaaatta attctggcgg ttaagataaa ttttacttca gatatacaat
     7141 tttttgccgg agatgaatgt acctcgcttt gaaactgacg aaataaagac cggaggcgct
     7201 accttgcggt tgccattggg ttttatattg accggctttg aataaaccat cttgtagaat
     7261 ggcgacttcc cggccggtta aatcgtaaat tgctagccgc ccggggatgt catacggcaa
     7321 atctaatttt atatttaccc attggttaaa aggattgggg taggacggat gaagccggaa
     7381 ctcctccggt cgagtctgcg gctccgagta gaaattgaaa taagtcgcat tctttaatac
     7441 ccaattgtct ggatcgaggg cgatggaatc cggtttaaaa ttgcagtaaa aagtgaaata
     7501 ttgggttgcc aaactgtcgg tgactataaa agttgtatca ttgaggctgt cactaaaaaa
     7561 caaagtcagc ggcattcgga aaattgttgc accggtctgg atttgtgaga tagcaacctt
     7621 taatttccag gaattaagag aatggcgata ggcttgccaa ttaacctgat aatgaggctg
     7681 accgactcca taaacccatt gctcgaagaa ccaatctaaa ctttggtcgt aaatttcttc
     7741 acactttttc tggaaatcgg cagtggtggc aaaaccgccg gagtattcct ggcggtatcg
     7801 cttaagaatt tcgaaaaatt tttcatatcc ggtcaagcct cttagcatat gcaataccca
     7861 ggcgccttta tcgtatacta tctgattgaa gatattgtta acgtcactgg tatcctgaac
     7921 atacaaacta ccggcgcgcc agcgagaagg gcgattcaga tccgtcatgt aattctttaa
     7981 tccaatggta ccttcatctt tttcccgcca aagtgcctct gcatagcggg caaagccttc
     8041 attaagccag atgtgcttcc agctggcgca agtaacatca ttcccccacc aggaatgcgc
     8101 taattcgtga caaatcagca tctcagaaaa cgatcccaga ctggtagcgg tttggtgctc
     8161 cataccaccg ccccaggaaa attgaacttg accgtatttt tctttgatga acggatattc
     8221 accccagagg gaagcaaagt gagtcatcat atcggcagtc atattcagaa acggacgagc
     8281 aattgtgtca aaatctggat aaatccaaaa ttccagcggc attgagctaa acccgtctat
     8341 accgaaataa ttttcactcc aataagtgta gtcggaaatt gctaatgaaa ttagataaac
     8401 ggcaatcggg tagcgttcct gccagcaata agtgcaggtg ccatcatcat tttcaattat
     8461 tctgaccaga gtgccgtttg aaacggcttt caattccgac ggtacggtaa tattaatgtc
     8521 tacggaatcg gctttatcac cgagccgatc tttgcaagga aaccaggtat gagcataata
     8581 gggttcgctc agagttgaaa tgaccggtgt tgaattgtga tattgaaaat atatccctct
     8641 gtcattattc tttttctcta agcaactata ggtaatcgag acgttgaagg tctcgttctc
     8701 gttcagaatt ttattcagtt gcaatgataa aatgttattg cgatgagtat agctgatggc
     8761 atctccgcca attgatgtta ccgataggtt gtcatccata tcgaggtcaa ttcgttgaag
     8821 gttagattca gcggctaatc cgcggatggt aacctcaccg tataaaattg agctggcaat
     8881 gtcgaaccga aaatccagag tgtaataagt tacgtcgtaa gcgtttgtat ttgcgtaact
     8941 cggtagctgg attagtgcgg ctcgctggtt ttccagccat acggttttag tacggatttc
     9001 ttgctcgatc caatcggagc taatttgagc tagagagaag gcagtgatgc ttataaggaa
     9061 aataatagtt aataaaaatc ttttcataag gcagtgaaaa gttaactatt gctgataagt
     9121 tttccagcgg tttcaatttt gcagttttgg agtgtaattt tggagtgcac cgaccgtgtc
     9181 ggtgcatgca gtgacatggt cactgcactc catatatgca ctccatagga actccatatt
     9241 cctttactct ataactacca ttttcttgtt caatattctt ttaaaatttg cagatttaat
     9301 ggttaatcgg taaatgtata gtccatagcg agacttttgt gttgccaatt aaccacatat
     9361 ctattcactc tttgccattc atgattaacc aaaatatcta ctttcctcgc cggtggtttc
     9421 aatccttggt ttagtggata aaggacttag acgtcgtata tcctcaagct atgagtgagg
     9481 aagaggagtt tcaatccttg gtttagtgga taaaggactt agaccttcac ttatttcagt
     9541 caaatcatca atcttacctc gtttcaatcc ttggtttagt ggataaagga cttagacgtg
     9601 ggaattgcgg gaagttaata gaagaatatg atggtttcaa tccttggttt agtggataaa
     9661 ggacttagac ttatctattc gccttttatt taccactatc gtttttagag tttcaatcct
     9721 tggtttagtg gataaaggac ttagacagtt cgggattgat tctttttgct tttgccccct
     9781 cggtttcaat ccttggttta gtggataaag gacttagacg atgctgaccg gacggagggg
     9841 ggattgctac cgcacagttt caatccttgg tttagtggat aaaggactta gactccggcg
     9901 ggttagcgtg tgaccacgac cgccggggac gtttcaatcc ttggtttagt ggataaagga
     9961 cttagacagt tcgggattga ttctttttgc ttttgccccc tcggtttcaa tccttggttt
    10021 agtggataaa ggacttagac gatgctgacc ggacggaggg gggattgcta ccgcacagtt
    10081 tcaatccttg gtttagtgga taaaggactt agactagttc agggttaaat cattcaccat
    10141 ttcattagtg ccgtttcaat ccttggttta gtggataaag gacttagact taagctagta
    10201 aggcggtcaa tccgggtggc tcaaagtttc aatccttggt ttagtggata aaggacttag
    10261 actgaatttt agttgaaatg ttagcggctt accaatcgtt tcaatccttg gtttagtgga
    10321 taaaggactt agaccaagct aataatgttg tcccactacc catatacgga tgtttcaatc
    10381 cttggtttag tggataaagg acttagacgt tcataagctg ggacgagcta acgtcgttga
    10441 aattaagttt caatccttgg tttagtggat aaaggactta gacgaacggt tacagtcttt
    10501 gaaggcaatg ctagctgcgt ttcaatcctt ggtttagtgg ataaaggact tagactttcc
    10561 gccgtgaacc ctaagaacgt tgtcataatt tgtttcaatc cttggtttag tggataaagg
    10621 acttagactt ctgccaactc attcacagtc agtggctgcc acttgtttca atccttggtt
    10681 tagtggataa aggacttaga cttcatacac cttcgccatt cgacctgttt tggcaggggt
    10741 ttcaatcctt ggtttagtgg ataaaggact tagacaccag acggcttttt tttatgctaa
    10801 tatcaatcaa gtttcaatcc ttggtttagt ggataaagga cttagactaa ccgttgaaat
    10861 cattgtaacc gtaaccgttg aaatgtttca atccttggtt tagtggataa aggacttaga
    10921 cgaggcaagc gtttttaggt caatatcttt aacgttaggt ttcaatcctt ggtttagtgg
    10981 ataaaggact tagactatac aattatttag gctggcaagt cgctaaacat tgtttcaatc
    11041 cttggtttag tggataaagg acttagacta agaaaaaccc gggatacaag aaccgccacc
    11101 agtcgtttca atccttggtt tagtggataa aggacttaga cgattattag gctatcagaa
    11161 ccaacaatat catctgaagg tttcaatcct tggtttagtg gataaaggac ttagacatgt
    11221 cgttcgtaat cttcgtgagt tcggatcata agtttcaatc cttggtttag tggataaagg
    11281 acttagacgg gggcttttcg tatttgttcc tcgctcagat acctacgttt caatccttgg
    11341 tttagtggat aaaggactta gacggtggta aagtcagatg tgtatattga ctttactagc
    11401 gtgtttcaat ccttggttta gtggataaag gacttagaca tatattgtat ctgtgtctaa
    11461 catagtggaa atatgtttca atccttggtt tagtggataa aggacttaga caagttcacc
    11521 aaagaattta ggttgcccaa tattttgttt caatccttgg tttagtggat aaaggactta
    11581 gacaataatc aggacggaag aaaaatgagt agaatctcag tttcaatcct tggtttagtg
    11641 gataaaggat ttagactata gccgttcata ggctatcaca attagatagg agagtttcaa
    11701 tccttggttt agtggataaa ggacttagac gtttgcgcca atttatgaaa taagcgcccg
    11761 ccaatcagtt tcaatccttg gtttagtgga taaaggactt agacaattct gtttggtgaa
    11821 tatggacgca taatttactc ctgtttcaat ccttggttta gtggataaag gacttagacc
    11881 tattaataag caaataactg ctatcgaatt attagagttt caatccttgg tttagtggat
    11941 aaaggactta gacagaagtg gagaaaggat ttcatgcaca ccaaatcagt ttcaatcctt
    12001 ggtttagtgg ataaaggact tagaccctaa agttaagtat ttccggattc ttgagtttac
    12061 gtttcaatcc ttggtttagt ggataaagga cttagacttt cccgagaaat gagtggattc
    12121 aaccgtttca tccaaggttt caatccttgg tttagtggat aaaggactta gactactgag
    12181 acgtttccgg aatgtggggg cggatcaagt ttcaatcctt ggtttagtgg ataaaggact
    12241 tagactggca caaccagaca caggattatt gggccttcaa acgtttcaat ccttggttta
    12301 gtggataaag gacttagact ggtggcggca gctggtgcag ctgaaatagc gactagtttc
    12361 aatccttggt ttagtggata aaggacttag acccagaccc actacgtatt ccttctcacc
    12421 ttcgctatgt ttcaatcctt ggtttagtgg ataaaggact tagacatata ttttcaagcc
    12481 tcgcttaccg caatactctt gtttcaatcc ttggtttagt ggataaagga cttagacctt
    12541 aatgcgccaa acattattga aatcccagcc gacagtttca atccttggtt tagtggataa
    12601 aggacttaga cgatttgtca gccaaagcga ttgacttggt tgttaagttt caatccttgg
    12661 tttagtggat aaaggactta gacaattctt tcagctctgg tttgaaatat atgtttgtag
    12721 tttcaatcct tggtttagtg gataaaggac ttagac
//
LOCUS       DAOSMP010000054        12581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_4998_length_12581_cov_12.272633, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000054 DAOSMP010000000
VERSION     DAOSMP010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 12581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12581
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4998_length_12581_cov_12.272633"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2101
                     /locus_tag="PKX09_08370"
     CDS             <1..>2101
                     /locus_tag="PKX09_08370"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOU17875.1"
                     /translation="NLPVISLSTDPYNLFDYNYGIYANGPGYTSADPHYGANFWMDWE
                     RPAHVEFYDDDKQLGFSKNCIIAIYGAWSRGFPQKSFSVKFKDGIEPLEYKLFPDLDL
                     TTYKAFVLRNSGNDFYNTHMRDAMMQTLIQDLDIDYLEYRPAITFINGRYWGIYNIRE
                     KINEHYVAYHHGVDKDNIDMLENNMEVIHGDTENYQQIINYIDTHDMSTDAAYNFIDS
                     TIDLNECLLYFAAQAYYNNRDWPGNNQKYWRARSGSKTKWRWIIYDLDFGFNLYESDG
                     HKEDHISFMFTVNNSNYAQAEWAIKLQKKLIENPTIRNRFINQIADLLNTNFKSERVV
                     NTINEMADHIADELPRHRQRFGIGGENRDKMITFAEQRPAYLRNHVRNYFKCGVDGNL
                     TIDITEGGTVKLNTLSLQAADAPFTGIYFQGNAVNLEAIPKPGYKFDGWSGAVTSSDV
                     SLSINVTISTNLVATFSAANDVVKDVVINEINYNSADDFDSGDWVEFYNNSDSSIDIC
                     NWFFKDNDDSHIFTFPSGTILGPSAYLVLGEDNEKFIACYPDVTNFVGEMDFGLSGSG
                     ELIRLFDSEGQIVDSLTYSDKSPWPTEADGQGATLELLDPNLDNTNGENWRASVGHGS
                     PGQQNTVTVGIAPNDSREIPEKFNLMQNYPNPFNHSTVISYQISEPSFVKLSVYDLNG
                     RLIETLVNEYRNPGYYAV"
     assembly_gap    2102..2201
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2202..3017
                     /locus_tag="PKX09_08375"
     CDS             <2202..3017
                     /locus_tag="PKX09_08375"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOU17876.1"
                     /translation="GNRIRDGKFSLEGKTYQLDINDGKNHLHGGKDGFYRQVWECREA
                     DSRSIQLRYISPDGEGGYPGQVEITVIYTITDDNGLKIEYSAKTDKTTILNPTSHCYF
                     NLSGFPEKSILDHELMINADYFTPVDAGLIPTGEIRLVAGTPMDFRVAKAVGKDIEAD
                     DEQILYGNGYDHNWVLNDYSGQVRLSATLYEKETGRLMEVLTDQPGLQFYSGNFLDGS
                     MTGKGGVKYNHRTALCLEAQAFPDSPNKPNFPSVVLRPGEEYKQTTIYRFSTK"
     gene            3045..4790
                     /locus_tag="PKX09_08380"
     CDS             3045..4790
                     /locus_tag="PKX09_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOU17877.1"
                     /translation="MLQTLDWIIIVGYFGIILGIAWWVMRQKQETSTDYFLASRNLGW
                     FIIGASIFASNIGSEHLVGLAGSGATDGVAMAHYELHAWCLLILGWVMIPFYMRSKVF
                     TMPEFLERRFSPRARTVLSLISLVAYVLTKIAVGIFAGGIVFSVLLPDLNFMGLDSFW
                     VGSVLVVVLTGLYTILGGLKAVAYTEALQTIVLVFGSILVTLFGLKALGGWGELREIA
                     GSEMFNLWKPLIPHGVEGTWAPIKESGRMAWYFNDNYPWIGMLLCAPIIGLWYWTTDQ
                     YIVQRALAAPDIKTARRGTIAAGYFKLLPVFIFIIPGIIAYALAVSGKIPALQSQLIG
                     PGGEVIRDNSQSAFPLLVSYVLPVGVRGIVVAGLLAALMSSLAGVFNASATLFTIDFY
                     SRFHPEASQKKLVWIGRVATAVMVLIGLIWIPVIRGGKGLYDYLQGVQSYLAPPIFVV
                     FFLGVFWKRANAKGCLATLITGFTLGLFRLAVDTPVKLFNVKYAEGSFLWIVNNIYFQ
                     YYSLLITIISILVMIFVSLGTKEPDYEKIKGLTYGTRTEEDRKELRANRSRGDVIASV
                     ILVILIVLCYIYFSG"
     gene            4824..>6514
                     /locus_tag="PKX09_08385"
     CDS             4824..>6514
                     /locus_tag="PKX09_08385"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOU17878.1"
                     /translation="MDKTKKYTIGLDFGTNSCRSLIVDISNGRELATHVFNYPSGEAG
                     IIVDSANPNLARQNPADYLLGLEVTIREALHKAAQIEPDFSPKDVIGIGVDTTGSSPM
                     PVDKEGTPLCFQSEFKDNPSAMVWLWKDHTSYAEAQKITELARQLRPQYLAKIGGIYS
                     SEWFWSKLWHLQSECPEVFRNTFSFVEICDWIPAVLTGNTDPLLIKRSICAAGHKALF
                     NEQWGGLPDEEFLKELSSEIAAMRQRLYRKAYSAEEKMGNLSPEWAEKLGLTTEVAVA
                     VGAFDAHMGAVGAGIATGILVKILGTSTCDIMVWPNSEKLNDIPGVCGIVDGSVLKGY
                     FGIEAGQSAVGDIFLWFVNNLVPDSFGKTQVEKFENLAKAAGGLKPGESGLLALDWNN
                     GNRTILVDVRLTGLLVGQTLHTQAHEIYRALIEATAYGALAIIDRIESYGVPVREIVN
                     CGGLAVKNPLLMQIYADVTGRPMKVSTSEQTPALGAAIFAAVSAGKAHGGYDGVTEAQ
                     RAMTSTGKEYLPDNRNHQTYKELYTLYKQMHDAFGTPEWSGKMFNVMKDLLNIRDR"
     assembly_gap    6515..6614
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6688..7383
                     /locus_tag="PKX09_08390"
     CDS             6688..7383
                     /locus_tag="PKX09_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HOU17879.1"
                     /translation="MLEKLKTEVLNANRSLVQYGLVILTWGNVSGIDREKNLVVIKPS
                     GIEYDKLTIDDLVVVNLDGRVVEGNRRPSSDTPTHLALYRAFDNIGGISHTHSDYATV
                     FAQAGCAIHCYGTTHADYFNGSIPITRLLTESEVNQNYEMNTGQVIIERFQDLNPLAM
                     PAVLVAGHAPFTWGKNATEAVKNSFILEKVAQLAYQSIMLNENMTELPKYILQKHYLR
                     KHGPSAYYGQNDK"
     gene            7402..8889
                     /gene="araA"
                     /locus_tag="PKX09_08395"
     CDS             7402..8889
                     /gene="araA"
                     /locus_tag="PKX09_08395"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HOU17880.1"
                     /translation="MKVFENYEVWFVTGSQHLYGEDVLRQVADHSREMAKYLSDSPNI
                     PAKVIFKSVLTEPEAILNLALEANSAKNCIGLIVWMHTFSPAKMWIAGLKNLQKPFVH
                     LHTQYNRDLPWSEIDMDFMNLNQSAHGDREFGFICSRMKINRKVIVGHWRDENVINEL
                     AIWVRAAVAWQDSRTGKIARFGDNMREVAVTEGDKVEAQIRFGYSVNGYGIGDLAQVV
                     QNIPEAEIDKLIEEYNAKYIVAREAAKGGEQYDALREAARIELGLRQFLKCGNFTAFT
                     TTFEDLYGLKQLPGLEVQRLMADGYGFGAEGDWKTAALVRAMKIMATGLTGGTSFMED
                     YTYHFNPSGMKVLGAHMLEVCESVAANKPRLEVHPLSIGGKADPPRLVFNAPTGPALN
                     VSLIDLGDRFRMILNEVESVQPDADLPKLPVARVVWIPKPNLKTAAQCWILAGGAHHT
                     GFSQALTADYLRDFAEIAGIELVEINNNTNVTEFKKELRWNEAVY"
     gene            complement(8973..10205)
                     /locus_tag="PKX09_08400"
     CDS             complement(8973..10205)
                     /locus_tag="PKX09_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17881.1"
                     /translation="MKNKQATTIIILFYLLIIANGSLAQVKNSIYSMYGIGQVIENNY
                     GINRAMGGTGIAFQSGNSINTINPASYLGVSPNSFTIEMGIYGIYNHASDGNITQTTG
                     DINISYFLADLYINNWWAISFGILPVSSVDYQINSSDQVNGELTTYEKTYSGNGGISQ
                     GYIGQSFKLTKRLTVGFNAAYYLGNLTQTEIGGGENSYTEYELENKKTINGLHFNYGL
                     QYSIEKADELYTLGLIYGSNKTLNTINDLSFTNTDTTISLEQNNQINIRIPRNFGLGI
                     SMKKGSNFRIGLDYQWKNWAQSNFNPSIFETKNSNRLSVGMEYSPNTRESKPFGQISY
                     RLGANYQNSYLAVNNVRINSFGVNWGIGFPMKRNNSLNFTIEYGEEGTLKKGLIKYSY
                     WAFYLHFSLHEFWSNQKR"
     gene            complement(10216..11523)
                     /locus_tag="PKX09_08405"
     CDS             complement(10216..11523)
                     /locus_tag="PKX09_08405"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HOU17882.1"
                     /translation="MVPRKSITYILSITCILAFYLKMCDENAENFTLGEEFIETQTDI
                     TLIDTFSVSLSTVLLDKVETSGTENMLIGNYEDKFLGKISCNSYFQIGIPTSIDIENE
                     DVYDSLYLVIYSNGYFRGDTTKPQGIYVYQLTENIVTETDEVLTNQSTFSYNPNPIGT
                     IIYNPKPTSAEDSVLIKINDNVGLDLFLKINEESDVISNNELFIDQFHGLVLKADEAY
                     TGSVVGFNVNSSDVKLVLCTTRFGEHGSEEIRNEFTMYDSTKQFNQITYDLSSTLLKP
                     LAEQRYELPTTNTHGLAFLQAGVGLIIRVDFPSLSNILMFEQGTIVKAELIVLSVDTG
                     NDDGDLNSSLLLYKSDKINRVQSSIASASKITTGESYEEEITYTFDITDYLKDELDDL
                     YFDPEESGLLITLSSTALSSTLERLIIDNKSPKTKLKIYYLTY"
     gene            complement(11587..12552)
                     /locus_tag="PKX09_08410"
     CDS             complement(11587..12552)
                     /locus_tag="PKX09_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HOU17883.1"
                     /translation="MSIGCDDSDDDDDDELIGNWFELSDFEGTPRSGAVVFCINDKAY
                     VGTGYDGTDRLNDFWKYDPFVDGWTRIADFPGSPRNGAVAFATDTKGYVGTGYDGKKK
                     LKDFWEYDPQTNSWTQIDSLIGTARYGAVAFAINNKGYIGTGYDGSALKDFYEYDPAT
                     GQWTQKLSLRGGKRQNAVAFVIDDKGYVCTGIDNASYETDLWEYDPTLDTWTRKRDIA
                     DVSDDDYDDDYSNISGIYKVSFNINGKGYIATGGYNSAGTYVWEYDPVVDLWEQKTSL
                     EASGRIEAVGFSIGNIGYITTGRNSSYFFDDLWGFNPNDEQVDND"
BASE COUNT         3589 a         2470 c         2673 g         3649 t
ORIGIN      
        1 cgaatttgcc ggttatctcg ctttccaccg atccctataa cttatttgat tacaactatg
       61 gaatttacgc aaatggtcca ggctatacgt cagccgaccc gcattacggg gcaaactttt
      121 ggatggattg ggaacgaccg gcgcatgttg aattttacga tgatgataaa caattgggct
      181 tctcgaaaaa ctgtatcatt gccatatatg gcgcctggag tagaggattc cctcaaaaat
      241 ctttttcggt aaaatttaaa gacggtatcg agccgctcga atataaactg tttcctgatt
      301 tagatctgac gacctataaa gcctttgtgc tcagaaattc aggaaacgat ttttataata
      361 ctcatatgcg ggatgcgatg atgcaaaccc tgatccagga cctcgatatt gattatctcg
      421 aatatcgtcc cgcaataacc ttcatcaatg gtcgatattg gggcatatac aatattcgcg
      481 agaaaatcaa tgaacattat gttgcttacc atcatggcgt agataaagac aatattgata
      541 tgcttgaaaa taatatggaa gtaattcacg gcgataccga aaattaccaa cagattataa
      601 attatatcga tactcatgat atgagtaccg atgcggctta taatttcatt gacagcacga
      661 ttgatctgaa cgagtgcctt ttatattttg ccgcccaagc ctattataat aatcgcgatt
      721 ggccaggcaa taatcagaaa tactggcgcg ccaggtcggg ttctaaaact aaatggcgct
      781 ggattattta cgatttggat ttcggtttta atctttatga atcggacggt cataaggaag
      841 atcatatatc ttttatgttt acggttaata atagtaatta tgctcaagcc gaatgggcaa
      901 taaaattaca aaagaaatta attgagaatc cgacgatccg gaaccgattt atcaatcaaa
      961 tcgccgattt gctcaatact aattttaaaa gcgaacgggt tgtcaatacc atcaatgaga
     1021 tggcagatca tattgccgat gaacttccca ggcaccggca acgatttgga attggtggag
     1081 aaaatcggga taagatgata acttttgccg aacagagacc ggcgtatctg cgcaatcatg
     1141 tgcggaacta tttcaagtgt ggtgtggatg ggaatctgac aattgatata actgaaggcg
     1201 gcaccgtaaa actcaacacc ttatcactcc aagccgcaga tgctcccttt accggaatct
     1261 attttcaagg aaacgcggtg aaccttgagg ctattcccaa acccggatat aaattcgatg
     1321 gctggagtgg agcagttacc agttccgatg tatcattaag catcaatgtc actatttcca
     1381 cgaacctggt tgcaactttt tcagctgcta atgatgtagt taaagacgtc gtcattaatg
     1441 aaattaatta caattctgcc gatgattttg attcgggtga ttgggtcgag ttttataata
     1501 atagtgattc ttcgattgac atatgcaatt ggtttttcaa agataatgac gacagccaca
     1561 tatttacttt tccgagcgga acgattctcg gaccctctgc ctaccttgtt ttaggcgaag
     1621 acaacgaaaa gtttattgct tgttatccgg atgtgaccaa tttcgtcggt gaaatggatt
     1681 tcggattaag cggaagcggt gaactaatcc ggttgttcga ttcagaagga caaatcgttg
     1741 attcactcac ctatagcgat aaatctcctt ggccaaccga agccgatgga cagggagcga
     1801 ctttagaact attagacccg aatttggata atactaatgg tgaaaattgg cgggcttccg
     1861 tcggacacgg ttcacctggc caacaaaata ctgtaacagt tgggattgct ccaaatgata
     1921 gtcgggaaat ccctgaaaaa ttcaatttga tgcaaaatta ccccaatccg ttcaaccact
     1981 ctacggtcat atcatatcaa atatcagagc cgtcgtttgt caaattatca gtttacgatc
     2041 tcaacggaag attaatcgaa acattggtga atgaataccg aaatcccggt tattatgcag
     2101 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggtaaccgc attcgggatg
     2221 gtaagtttag tttagaggga aagacgtatc agcttgatat taatgatggt aaaaatcatc
     2281 tacacggtgg aaaagacgga ttttatagac aggtttggga atgtcgcgag gctgattcca
     2341 ggtcaattca gctacgttac atcagtccgg atggggaagg cggatatccg ggacaagttg
     2401 aaataactgt tatttataca attacggatg ataatggact caagatcgaa tatagtgcca
     2461 aaaccgacaa gacgacgatt ttgaatccaa ccagtcattg ctatttcaat ttgagtggat
     2521 ttccggaaaa aagtatctta gatcacgaac taatgataaa tgccgactat tttacgccgg
     2581 tggatgccgg tttaattcca accggtgaaa ttcgacttgt tgcaggtacg ccgatggatt
     2641 tccgagttgc aaaagcggtt ggcaaagaca ttgaagccga tgatgaacaa attttatatg
     2701 gcaacggcta cgatcataac tgggttctga acgactatag cggtcaggtt cgactttcgg
     2761 cgactttgta cgaaaaagaa actggccgat tgatggaagt tttaaccgac caaccgggtt
     2821 tgcaatttta ttctggaaat ttcctggatg gttcaatgac cggcaaggga ggggtaaagt
     2881 acaatcaccg tactgctctt tgtctggaag cccaggcttt tccggattcg cccaataagc
     2941 ctaattttcc gtcggtggtt ttaagacccg gtgaagaata taagcagacg acaatctatc
     3001 gtttttcgac aaaatagttc aacttttaac aaggggaaat agctatgtta cagactttag
     3061 actggataat cattgtaggc tattttggaa tcattctcgg aatagcttgg tgggtaatga
     3121 gacagaagca agaaacatcc acagattact ttcttgccag ccgcaatttg ggctggttca
     3181 tcattggagc ttcaattttc gcttcgaata tcggttcgga gcatttagtc ggactagcag
     3241 gttcgggagc gaccgacggc gttgctatgg cgcattacga attacatgcc tggtgcttat
     3301 taatccttgg gtgggtgatg atacctttct atatgcgttc gaaagtattc acgatgccgg
     3361 aatttttgga aagacgtttc tcgcccagag ctcggacagt tttgtctcta atttcgttag
     3421 tggcttatgt gctgactaaa attgccgtag gaatttttgc cggtggtatt gtatttagtg
     3481 tgctattgcc ggatttgaat tttatgggat tagatagttt ctgggttggt tcggtactgg
     3541 tagtggtgtt gaccggatta tatacgatat tgggcggttt gaaagccgtt gcttataccg
     3601 aagccttaca gacgatcgtt ttggttttcg gttcaatttt ggtaaccctg ttcggtctaa
     3661 aggctctcgg cggttgggga gaattgcggg aaatcgccgg ttcggaaatg tttaacctct
     3721 ggaagccact cattccccat ggcgtcgagg gcacctgggc gccaatcaaa gaaagcggcc
     3781 ggatggcttg gtatttcaat gataattatc cctggatagg aatgctgtta tgcgctccaa
     3841 ttattggatt atggtattgg acaaccgacc aatatattgt acagagagca ttagcggctc
     3901 cagatataaa aacagcgcga cgtggcacca ttgccgccgg gtactttaaa ttattaccag
     3961 ttttcatttt cataatacct ggtataattg catacgccct ggcagtcagt ggtaaaattc
     4021 cagcccttca gagccagcta atcggaccgg gaggtgaggt tatcagggat aattctcaga
     4081 gcgcctttcc actcttagta tcttacgtct tgccggtcgg ggttcgaggt atcgtcgttg
     4141 ctggattatt agcggcgtta atgagttcct tagcaggcgt tttcaacgct tcggcgaccc
     4201 tttttacgat tgacttttac tcacgctttc atccggaagc tagtcagaag aagctggttt
     4261 ggattggccg ggtagctact gccgttatgg tattgattgg tctgatatgg attccggtaa
     4321 ttcggggtgg aaagggactt tatgattatt tacaaggtgt tcaatcttat ttagcaccgc
     4381 ctatttttgt ggtctttttc ttgggagttt tttggaaacg ggcgaatgca aaaggctgtc
     4441 tggctacctt gataaccggc ttcacccttg gtctcttccg actggcagtt gatacaccgg
     4501 taaaattgtt taatgtcaaa tatgctgaag gttctttcct gtggattgtg aataacatat
     4561 acttccaata ctacagcctt ttgataacga taatatcaat tctcgttatg atttttgtta
     4621 gtttgggcac aaaagaacct gattatgaaa agatcaaagg tttgacctat ggtaccagaa
     4681 ccgaagagga tcgcaaggaa ttacgtgcta atcggtcacg tggcgatgta atcgcttcgg
     4741 tcatattagt aattctgatt gttctgtgct atatctattt ttcgggttga tattcaagca
     4801 gttaacgtta tttactggac acaatggata agacaaaaaa atataccatc ggattagatt
     4861 ttggcactaa ctcttgccgt tcgctgattg tagatatttc taacggccgg gaattagcga
     4921 cacatgtttt taattatcct tcgggtgaag ccgggattat tgttgattcg gcaaatccta
     4981 accttgctcg ccagaacccg gctgattatc tgctgggctt agaagttaca attcgagagg
     5041 cgcttcataa agccgcccaa attgaacccg atttttcacc taaagatgta attggaatcg
     5101 gagtagatac taccggaagt agtccaatgc cggtagataa agaagggaca ccgctctgtt
     5161 ttcaatctga atttaaagat aacccttcag ctatggtctg gctctggaaa gatcatacca
     5221 gttatgccga agctcaaaaa ataactgaac ttgctcgtca attacggcca caatatttag
     5281 caaaaatcgg tggcatttac tcatcggaat ggttttggag caaactctgg catctgcaaa
     5341 gcgaatgtcc ggaagttttt cggaacactt ttagttttgt cgagatatgc gactggattc
     5401 cggctgtatt gacaggcaat accgacccat tactaattaa gagaagcatc tgcgccgccg
     5461 ggcataaagc cctgttcaac gagcagtggg gcggtttgcc tgatgaagaa tttttgaagg
     5521 aactatcttc ggaaatagcc gcaatgcgtc agcgccttta ccggaaagcc tattcggccg
     5581 aagaaaagat gggaaatcta tccccagaat gggcggaaaa attgggattg acaactgaag
     5641 tggctgttgc cgttggagct tttgatgccc atatgggagc agttggagct ggaattgcga
     5701 ccgggattct ggtgaaaatt ctgggaacca gcacttgcga tataatggtc tggccaaata
     5761 gcgagaaatt gaatgacata ccaggagtat gcgggatagt agacggttca gttctaaaag
     5821 gctatttcgg tatcgaagcc gggcaatcag cggtgggcga tatttttctg tggtttgtta
     5881 ataatctagt gcctgattcc ttcggtaaaa ctcaagtaga aaagttcgaa aatctggcga
     5941 aagccgctgg cggtctaaaa cccggcgaat caggtttgct ggcactcgac tggaataatg
     6001 gcaatcgcac tattttggtt gatgttcggt tgactggttt attggttggt caaactttgc
     6061 atactcaagc acacgaaatt tatcgagcat taatcgaagc gacggcttat ggcgctttgg
     6121 caattattga ccgaattgaa agctacggcg ttcccgtacg agaaattgtc aactgcggtg
     6181 gtttggcagt caaaaatcca ctattgatgc aaatatatgc cgatgtcacc ggccgaccga
     6241 tgaaagtttc gaccagtgaa caaactccgg cgctgggcgc agctatcttt gcggcggttt
     6301 cggctggcaa agcgcacggt ggatatgatg gtgttactga ggcccagcga gcgatgacca
     6361 gcaccggcaa ggagtattta cccgataacc gtaatcacca gacatacaaa gagttatata
     6421 ctctttataa acagatgcat gatgcttttg gtactccgga atggtcgggg aagatgttca
     6481 acgttatgaa agatttgtta aatattcgcg accgnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnaatggt cggggaagat gttcaacgtt atgaaagatt tgttaaatat
     6661 tcgcgaccga gtaaggcagg agaaattgtg ctcgaaaaac taaaaactga agttctgaat
     6721 gccaaccgat ctctggtgca gtatggattg gtgattttaa cctggggcaa tgtcagtggc
     6781 attgatcgtg agaaaaatct agtcgttatc aaaccgagtg gtatcgaata tgacaagttg
     6841 acgattgacg atctggtagt agtgaatttg gatgggagag tagttgaagg taatcgccgt
     6901 ccttcgtcgg ataccccgac acatctggct ttatatcgag cgtttgacaa cattggcgga
     6961 atcagtcata ctcatagtga ttacgctacg gtcttcgctc aagccggttg cgcaattcat
     7021 tgctatggca cgacgcacgc cgattatttc aatggttcaa ttccaatcac tcgcttatta
     7081 actgaatcgg aagttaatca gaattacgaa atgaacaccg ggcaggttat catcgagcgt
     7141 tttcaggatt tgaatccgct cgcaatgccg gccgttctag tagctgggca tgcacctttt
     7201 acctggggaa aaaatgctac cgaagcggtt aaaaatagct tcattctgga aaaggttgcc
     7261 caactggcat atcaaagcat tatgctgaat gaaaatatga cggagttacc caaatatatc
     7321 ctgcaaaagc attacttacg gaaacatgga ccatcagcct attacggtca aaatgataaa
     7381 taatcaataa aggaataaaa aatgaaagta tttgaaaatt acgaagtttg gtttgttacc
     7441 ggcagtcagc acctgtacgg agaagatgtt ttgcgccagg tagccgacca ttcccgcgaa
     7501 atggctaagt acctatctga ttcgccgaat attcctgcca aagtaatttt taaatcagtt
     7561 ttgactgaac cagaggcgat tttgaatctt gcgttggaag ccaacagcgc caaaaattgt
     7621 atcggtttga tagtctggat gcatactttt tcaccggcaa aaatgtggat cgccggattg
     7681 aaaaacctac aaaaaccatt tgttcatctg catactcagt ataaccgcga tctgccctgg
     7741 tcggaaattg atatggattt tatgaatctg aatcaatcag cccacggcga tcgcgaattc
     7801 ggcttcattt gttcccgaat gaaaatcaac cgcaaggtga ttgtcgggca ctggcgcgat
     7861 gaaaacgtca taaatgaatt ggcaatctgg gtccgcgccg cggtcgcctg gcaagatagc
     7921 cgcaccggga aaatcgcgcg ctttggcgat aatatgcgcg aggttgccgt cacagaaggc
     7981 gacaaagtcg aagctcaaat ccggttcgga tattcggtaa atggctatgg aataggcgat
     8041 ttggcacaag tagttcagaa tattcctgaa gccgaaattg ataaattgat cgaggaatat
     8101 aatgctaagt atattgtcgc cagagaagcc gctaaaggtg gcgagcaata cgacgctctg
     8161 cgagaagccg cccgtattga actgggtttg cgacaatttc tgaaatgcgg caattttacc
     8221 gcatttacga caaccttcga ggatttgtac ggtttgaagc aacttcccgg tttggaagtc
     8281 cagcgcttaa tggcggacgg ttacggtttt ggagcggaag gcgattggaa aacggccgca
     8341 ttagtgcggg cgatgaaaat tatggcaacc ggcttaaccg gtggcacctc atttatggaa
     8401 gattatactt atcatttcaa tccatcagga atgaaagtct taggcgcaca tatgctggaa
     8461 gtatgtgaat ctgtcgccgc caataaaccc cgcttagaag ttcatccact ctcaatcggt
     8521 ggcaaagctg acccgccgcg tttggtattc aatgctccga ccggtccggc gctgaatgtt
     8581 tcgctcattg atctcggtga tcgttttcgg atgattctca acgaagtcga gagtgtccaa
     8641 ccggatgccg atttaccaaa actaccggtt gcccgggtgg tctggatacc caaacctaac
     8701 ctgaagactg ccgctcaatg ctggattcta gcaggtggag ctcatcatac cggcttcagt
     8761 caagccttga ccgcagacta tctacgcgac ttcgccgaaa ttgccggaat cgaactggta
     8821 gaaatcaaca acaacactaa tgttaccgaa ttcaagaaag aactgcgttg gaacgaggcg
     8881 gtgtactgag ttacgaacat taaagggatg atgactcaaa gaatgttgta atataaaact
     8941 atcaatctgc cggattattg tttataaagc gactatcttt tctgattaga ccaaaactca
     9001 tgtaatgaga aatgtaaata gaaagcccaa taactatatt ttatcaagcc ttttttcaag
     9061 gtgccttctt ccccatattc gatagtgaaa tttaaactat tattacgctt cataggaaac
     9121 ccaatacccc aatttacacc gaaagaattt attctaacat tatttactgc aagataggaa
     9181 ttttgataat tagcgcccag ccggtatgaa atctgcccaa atggctttga ttctctcgtg
     9241 tttggagaat attccattcc taccgaaagc cgattactgt tttttgtctc aaaaatagat
     9301 gggttaaaat tactttgagc ccaatttttc cactgatagt ccaaaccgat tctaaaatta
     9361 ctcccttttt tcattgatat tcccaaccca aaatttcggg gaattcgtat gttgatttga
     9421 ttattttgtt ctaatgaaat tgtagtatca gtattggtaa aacttaaatc attaatagta
     9481 tttaatgtct tgttagagcc gtaaataagc ccgagagtat atagctcatc cgctttttcg
     9541 atcgaatact gcaatccgta attgaaatga agaccattta tagtcttttt attttctaat
     9601 tcatactcag tataactatt ttcaccgcca ccaatttctg tttgagttaa attacctaag
     9661 taataagcgg cattaaatcc cacagtcagt cgttttgtta atttaaaaga ttggccaatg
     9721 tagccttgac tgataccgcc gtttcctgaa taggtttttt catacgttgt caattcacca
     9781 ttgacttgat cacttgagtt aatttggtaa tcaacggaac taaccggaag aatgccaaaa
     9841 ctaattgccc accaattatt gatatacaaa tctgctaaaa aataactgat gttaatgtca
     9901 ccggtagttt gagtgatatt gccatcgctt gcatgattgt atattccata aattcccatc
     9961 tcgatggtaa aagaattagg tgagacaccc aaataggagg ctggatttat agtattgatc
    10021 gaattcccag attgaaatgc gatgccagtt cctcccatcg ctcgatttat gccgtaatta
    10081 ttctctatga cttgtccaat tccgtacatc gagtagatgg aatttttcac ctgagctaat
    10141 gacccatttg ctataattaa taaatagaat aatataataa tagtagtggc ttgtttattt
    10201 ttcattattt ataaatcagt aggtcaaata atatattttt aattttgttt ttggactttt
    10261 gttatcaata attaatcgct ccaaagtaga gctaagagcg gttgatgata atgtaataag
    10321 tagtccagac tcttctgggt caaaatataa atcatccagt tcgtccttta aataatcagt
    10381 aatatcaaat gtataggtaa tttcttcctc ataagattct ccggttgtaa tttttgatgc
    10441 gctagcgata ctactctgta ctctattaat tttatccgat ttataaagaa gtaaactaga
    10501 attgaggtct ccatcatcat taccagtgtc cactgataaa acaattagtt cggcttttac
    10561 tatcgtacct tgttcaaaca tcaatatatt ggacagactg ggaaaatcca ccctgatgat
    10621 tagtccaact ccggcttgta aaaatgctaa accgtgggta tttgtagttg gtaattcgta
    10681 tcgttgttcc gcgagcggtt ttaaaagggt agaagataaa tcataagtga tttgattaaa
    10741 ctgcttggta gaatcgtaca ttgtaaattc atttctgatt tcctcactcc catgttctcc
    10801 aaacctggtg gtacacaata ccaattttac atcactactg ttaacattaa aaccgaccac
    10861 tgatcctgta taggcttcat cggccttcaa taccagtccg tgaaattgat caatgaaaag
    10921 ttcattgtta ctgataacat cagattcctc attaattttt aaaaataaat ctaggccgac
    10981 attatcgttt attttgatga gaacactatc ttctgcgctg gttggtttgg gattatagat
    11041 tatagtaccg attggattag gattataact gaaagttgat tgattagtca gaacttcatc
    11101 cgtttcagtt actatatttt cagtcaattg ataaacgtat attccttgag gtttggtcgt
    11161 atcgcctcga aaatacccat tagaatatat aaccaggtat agtgaatcat aaacatcttc
    11221 attttcaata tcaatgctcg tcgggatacc gatttggaaa taactgttgc atgaaatttt
    11281 ccccaggaac ttatcttcat aattaccaat gagcatattt tctgtgccgg acgtttctac
    11341 cttatctaga aggacagtag agagactgac cgagaatgta tcaattaagg taatgtctgt
    11401 ttgggtttcg ataaattcct cacccagagt aaaattttcg gcgttttcat cgcacatctt
    11461 taagtagaaa gccaaaatgc aagtgattga taaaatatag gttattgatt ttctcggtac
    11521 catcataatt ttattgtttt aaaagtagta ttgcggcagt atatcgtttt aaatgaataa
    11581 tgatatttaa tcattatcta cttgttcatc gtttggatta aatccccata aatcatcaaa
    11641 aaaataactg gaattccttc cggtagtaat atatcctata tttccgatag aaaagccaac
    11701 agcctcgatt cttcccgaag cttccagtga agtcttttgt tcccacaaat ctactactgg
    11761 atcatattcc caaacgtatg taccggcaga gttatacccg ccagtggcaa tgtagccttt
    11821 gccgtttata ttaaacgaaa ctttataaat accagatata ttagagtaat catcatcata
    11881 gtcatcatca gaaacatcgg caatatcacg ttttctggtc caggtatcta aagtcggatc
    11941 atattcccat aaatcagttt cataagacgc attatcaata cccgtgcaaa catatccttt
    12001 atcgtctata acaaatgcca cagcattctg acgcttccca cctctaagac taagtttttg
    12061 ggtccattga ccagttgccg gatcatattc ataaaagtct tttaaagcac taccgtcata
    12121 cccggtacca atatagcctt tattattaat cgcaaaagcg acagctccat atcttgctgt
    12181 acctattaga ctatcaattt gtgtccacga gttagtctga ggatcatatt cccaaaaatc
    12241 cttcaatttt tttttgccat catacccggt gccgacatag cccttagtat cggtagcaaa
    12301 ggccacagcg ccattccgtg gactacccgg aaaatcggct atgcgcgtcc aaccatctac
    12361 gaacggatcg tatttccaaa aatcattaag cctatcggta ccatcataac cggttccgac
    12421 gtatgcctta tcattaatgc agaacactac ggcccccgag cggggagttc cttcaaaatc
    12481 agatagctca aaccaattgc caatcaattc atcatcgtcg tcatcatcac tatcatcaca
    12541 tccaatagac attaaaatga ggggaaatac tacaataccc a
//
LOCUS       DAOSMP010000055        11772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_5412_length_11772_cov_13.211744, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000055 DAOSMP010000000
VERSION     DAOSMP010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 11772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11772
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5412_length_11772_cov_13.211744"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1483)
                     /locus_tag="PKX09_08415"
     CDS             complement(<1..1483)
                     /locus_tag="PKX09_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOU17884.1"
                     /translation="MLNYVKSALKNSAIYSIGNVSTKLIGIILVPLYTQHLSLKEYGI
                     LSILEVSSQLLVTIFSANLYTAFFRWYWEKEYSDRQKSIFFTTFLSLIFISGLMVAGM
                     YGFSSRLSVLLFDNSDFNYLIRLMSIAAALQILMDLVSALMRVQEKPVLYTSVNVAKL
                     TISLILTVYFISSLHMGIDGIYKAQIISYIVYFILVSRYLIKNIRVRLEIRVLKEMIR
                     FSLPLMISSVSGILLSIADRFCLKFLGELSYVGIYSLGFKIANTVKVFIISSVNLAIS
                     PVMYRVMDDPGNKRLYSKTMTYFAFGTMICVIGISVFGQEIIKFLAQNEAYWNAYKVI
                     PIISFGLFFEMLKDTSLIGLNITKKTPVIARIIITSSIINIALNLLLIPYFKYIGSAL
                     ASLVTQIIYFLLIYHSAQKYYPIPYEMEKIGKIMLCGIVLIGFGLILSPYALWIRLTG
                     KLLLILLFPFILYLWNFYEPIEIQTIKGAWRKWRNPLNWKKNRS"
     gene            complement(1535..2080)
                     /locus_tag="PKX09_08420"
     CDS             complement(1535..2080)
                     /locus_tag="PKX09_08420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOU17885.1"
                     /translation="MTSFYNEEEIIKLGLKKYGKNVLISRKASLYSPELIQLGNNVRI
                     DDFCILSGNISIGSYVHISAYTALYGKFGICIKDFVTISGRVLVYSQTDDYSGDFMTN
                     PMVNPNLTRVTGGPVIFQKHSIVAAGCIIFPEVTIGEGAAIGAMSLVNKNLEPWSIYV
                     GIPAKYLKPRSKKIIELEHLI"
     gene            complement(2093..3178)
                     /locus_tag="PKX09_08425"
     CDS             complement(2093..3178)
                     /locus_tag="PKX09_08425"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOU17886.1"
                     /translation="MTMINVTKTYLPQKQKYLEYINKIFKSGWLTNNGELVQNLERRL
                     EDYLGVPHVILTANGTLALQIAYHLLELKGEVITTPFTFIATTSSLVWEKLIPIFADI
                     EPDTFNIDPIQIEKNINNNTSAILGVHVFGNVCNVGKIEEIANSYKLKVIYDAAHAFG
                     VKYYNKSISEFGDVSIFSFHATKIFHTIEGGAIVLKDDDMAKKARLLINFGIPGPDEI
                     ATLGINAKMNEFQAAMGLCVLDDIQDIIEKRHVIYNRYLEAFIDNNKLTLQQIAPHST
                     QNYSYFPVLFESEKILLRIKQSLNQAEIFPRRYFYPSLDTLHFIHPKQKMPISRNLSS
                     RILCLPLYEDLTEDIQDQIIKVILMYC"
     gene            complement(3175..4266)
                     /locus_tag="PKX09_08430"
     CDS             complement(3175..4266)
                     /locus_tag="PKX09_08430"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: protein motif:HMM:NF019073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0036065 - fucosylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HOU17887.1"
                     /translation="MLTVHIFTCENLYNYQFINFIHKHFDISKHFFVYRASHNKQYEY
                     PPSFQIKNILYIDSKSKYILKLIPLLLRSQIIYIHFLPVGITPIIWLIFPSLIPKTSW
                     ILWGDDLYWVETRKKGFKAAIYESIRRRVIVKLPRIIAFMEGDYRIAKSRYNTRAIYQ
                     YAVYPLPTDFEYLESIRCNDLVNEEITIQVGNSGDPSNDHIGVLEHLKRFSDRHFKIF
                     CPLSYGGTKEYINSVIQYGQDAFGEKFSPLLEVIQPKDYSKLLLKVNVAIFGSSRQQG
                     LGNILSLLFLGKKVYIRKETTPYEYFKKINVVIFPYSDLENVSFCELAYISPLIAENN
                     YKIIKNHFNEENYVRMWKEVLSIDTNKRK"
     gene            complement(4298..5503)
                     /locus_tag="PKX09_08435"
     CDS             complement(4298..5503)
                     /locus_tag="PKX09_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU17888.1"
                     /translation="MRIGMILDKEFPPDERVENEALILLEGGHEVYVLCYGYSRSQTK
                     IENYRGIVIQRIPTSKCLIKRLRVFQHPSLNLYKNFWERKVKKFVVHNHIDVLHIHDL
                     YMVPSGIKARRYIGIQVVVDLHENYPAALKAYTWANTLTGRLIIRPQDWEDIEAVYFQ
                     NVDRIIVLSQYYRIQLLRKYTFLKDDAITVYPNVPCVKGLLNYKIRRVLDKEDNFTLL
                     YFGVIARRRGIFTVFEALRILIQQGRQKIKLLLIGPIDTNEKKEFNKYLSDNTLKDQI
                     IHYRWKDISEMPSYITDSDVCLSPILKNDQHESGIANKVFQYMLFARPVIVSDCGPQK
                     DLIETTGCGLVHKSDNAIDLAMKIQYLQDNPELRKTMGEKGRKAVLEIYNTEIQGRQL
                     IELYKAINV"
     gene            complement(5500..6276)
                     /locus_tag="PKX09_08440"
     CDS             complement(5500..6276)
                     /locus_tag="PKX09_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17889.1"
                     /translation="MSIKTFDIVRKIRQKSRLGVYRELLIFAQKEGYIITSLIEWFHK
                     YCQNGQRVLILRHDVDIDTEGAAKMYAIEKSLGVHATYYFRWKTVSEALVYKMRRDGF
                     EASLHYETLATYCKKHNIKDAKRVTDTVMKTCFNLLEKEISRFKTQFGGIQTIASHGD
                     KRNRILGIPNHAILKYGNRADLDIQFEAYDPEITMLFDAYISDSSITNNFNWKYGLTP
                     QEAIRSHLTTICLLTHPEHWNFNFKKNMKTIITDICERLS"
     gene            complement(6373..8307)
                     /gene="asnB"
                     /locus_tag="PKX09_08445"
     CDS             complement(6373..8307)
                     /gene="asnB"
                     /locus_tag="PKX09_08445"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOU17890.1"
                     /translation="MCGICGIVDPNENNHRLITEMTDLLKHRGPENVGYFHNGNVSLG
                     HRRLAIIDLETGDQPIYNEDRTVVVIMNGEIYNFQEFKADLITRGHHFYTNSDVEILS
                     HGYEEYGLEFLAHLNGIFAFALYDIKNERLILARDHFGVKPLHYWFQNEVLLFSSEYK
                     AILVHPAMRRQVNYSALHCQVNLRYNQSDETLIKGIYRLPPASYLIYENGQVSIKKYY
                     QLPVNLNFSMTESEAIDGIRFHLQQAVRRQLISDVPLGVYLSGGMDSSALVAMMAAEK
                     IRPINTFTLGFNEATDEFDDAAVIARQFNTNHTTTALALEPLQYFPETIRHAEEPKIN
                     LLQGFHLSEFVSHSLKVVLGGLGGDELFVGYDIYRYLWAMRGLLQNRPGFCQKTLWDS
                     IGTLLFSLQNSTKILKWDEYRRGLQMLFSAGDLEKCYLILRNCWDYDMRFAKEIYQPE
                     FLQTTLAPTRTWFDPIFAACRDLPPLDAIAYVEFHSKMLNDYILTEDRMSMAHSLELR
                     VPFLDKDLVEFAFTIPATLKMKNGQTKYLFRKAMAPYLPPEILQKKKRGFAVNPYDQF
                     NKDLKKVAEKVLTREFIQHQGIFNYEYLQRILNYPPDPRLRWHYNFLWIVLGLAIWER
                     MFIETEKYRQPVVTPQEYYT"
     gene            complement(8397..9491)
                     /locus_tag="PKX09_08450"
     CDS             complement(8397..9491)
                     /locus_tag="PKX09_08450"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOU17891.1"
                     /translation="MIPFMDLETQYLSIRQEIDSAIFNVIHSYKFINGPEVATFEKNF
                     ATAQNLKYMLGLASGTAALHLAYEILGITSGDEVIVPAMTFIATAETVRQVGAKPVFA
                     DIDPVSFNIDCNSLEKLVTPNTKAIVVVHLHGNPCEMDRVSSFARKYNLKIIEDCAQA
                     HLAEYQNQKVGTFGDVAAFSFYPGKNLGAYGDAGALGTNNETLYEKAKLLANHGRKEK
                     YVHQTEGYNYRLDTLQAAILDVKLRHLPEWTEQRINIANTYYSLLKNLPITLPEMNPA
                     KKHVYHIFAIATDRRQKIIEALKANQIAFGIHYPLPLHLQPAYSYLHYKQGDLPYSEQ
                     LSTQFLSLPMYAELSRDDIEKICQVVASVY"
     gene            complement(9507..9971)
                     /locus_tag="PKX09_08455"
     CDS             complement(9507..9971)
                     /locus_tag="PKX09_08455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOU17892.1"
                     /translation="MAEPIIKKIGIRDIKCGTNVVIIEPCNLYECEIGDNTFIGPFVE
                     IQKGVKIGRRCKIQSHSFICELVTIGDDCFISHGAKFVNDTFKSGKPAGGNRELWKPT
                     IIGNHVAIGTNATVLPVKITDRVIIGAGAVVTKDILHPGIYVGNPARKIRDL"
     gene            complement(9974..10984)
                     /locus_tag="PKX09_08460"
     CDS             complement(9974..10984)
                     /locus_tag="PKX09_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU17893.1"
                     /translation="MKTIKFALIGCGRIAQRHAEQIHRLGELSAVCDIKESAALTLSE
                     KYNCRPYFDIDTLLKNEPDVDVVSVCTPNYLHAEHSIKSLKAGKHVLCEKPMALSVRD
                     CENMIYTAEQTNRRLFIVKQNRYNPPVVELKKVIEGNRLGKIFYVQLNCFWNRNEDYY
                     LQSDWKGRLDKDGGTLYTQFSHFIDLLVWLFGDFKNVKVLLQNYLHPNIEFEDTGAVL
                     IEFCNGAIGTINYTVNSYQKNMEGSITVFGSKGTVKIGGQYLNALEYQNIEDYSFQNV
                     TPVNKANDYGFYQGSMSNHDKVYENVVNVLNGQDTIATNAIEGLKVVRIIEDIYSQSK
                     LK"
     gene            complement(11025..11150)
                     /locus_tag="PKX09_08465"
     CDS             complement(11025..11150)
                     /locus_tag="PKX09_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17894.1"
                     /translation="MKELIKDKTNAGNMSEVAITGKTYYMPYFLSVNCEYPETSS"
     gene            complement(11225..>11772)
                     /locus_tag="PKX09_08470"
     CDS             complement(11225..>11772)
                     /locus_tag="PKX09_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17895.1"
                     /translation="GSISIEQDIQAFCKAIEVLIQNENIRENIIIQFIGNFPEFVKQK
                     LRQYSVFEMINFLPYMPADEVKQYLVSSDVLLLFIHVTPEGGVINYKMYDYLATYKPI
                     LAYGPTQGDAAKILRRANAGRMFEYDNIADSANYLLSLYEKWEKGEPAIEIDKEYVMT
                     FSRRNLTRQLAEIFDKYYRKS"
BASE COUNT         3640 a         2309 c         2079 g         3744 t
ORIGIN      
        1 tagaacggtt ctttttccaa tttaaaggat tacgccattt gcgccaggcg cctttaatgg
       61 tttgaatctc aataggctca tagaaattcc ataagtataa gataaatgga aataatagaa
      121 ttaatagcaa ttttccagtt agccgaatcc acaatgcata gggtgataag attagtccga
      181 atccaattaa tactattccg catagcatta ttttgccaat tttttccatc tcgtaaggta
      241 tgggatagta tttttgagca gagtggtata ttaaaaggaa ataaattatc tgcgtaacca
      301 gtgatgccag cgctgaaccg atatatttga aatatggtat aagtaaaaga tttagagcaa
      361 tattgataat cgaagaagtt attattattc gtgcgataac gggtgttttt ttagttatat
      421 ttaatccaat tagcgaagtg tctttcaaca tttcgaaaaa caaaccaaac gaaattatcg
      481 gaattacctt atacgcattc cagtaagctt cattttgcgc caagaatttg ataatttctt
      541 ggccaaagac ggaaattccg ataacacaaa tcatagttcc gaacgcgaaa taggtcatcg
      601 tcttggaata aagacgttta ttaccgggat cgtccatcac tctatacata acgggcgaaa
      661 tagccagatt taccgaacta atgataaaga ccttgacggt attggctatt ttaaaaccaa
      721 gcgagtagat tccaacgtaa gagagctctc ctaagaattt caaacaaaat cgatcagcaa
      781 tacttaataa tattcccgat acagaagaga tcattaaggg cagactaaaa cgaatcattt
      841 cctttagaac tcgtatttcc agacgaaccc gtatattttt aatcaaatat cttgatacta
      901 aaataaaata aacgatatag ctgataatct gggctttata tattccatct atccccatat
      961 gcaaactgct gataaaatat actgtcagaa taagactgat tgtcaatttt gcgacattga
     1021 cggaagtata taaaaccggt ttttcctgaa ctcgcataag tgctgaaact aagtccatta
     1081 gaatctgtaa agccgccgca atggacatta atcttattag ataattaaaa tctgaattat
     1141 cgaacaataa aacagataac cgactggaaa aaccgtacat cccagctacc ataagtcctg
     1201 agataaaaat tagagataaa aacgtagtaa aaaatattga cttctgacga tcgctatatt
     1261 ctttttccca gtaccaacga aagaaggctg tatataagtt cgcactgaat atcgtaacta
     1321 gcagttgcga actaacctct aggatgctta gtattccata ttcctttaga gataagtgtt
     1381 gagtatatag cggaacaaga ataattccga tcagttttgt ggaaacgtta ccgatactgt
     1441 aaatagcgga attctttaaa gccgatttga cgtaattaag cattgggaag tgttacaata
     1501 acgagtacac aattaaataa aaattaaatt aattctaaat cagatgctca agttcaatta
     1561 ttttttttga tcttggtttt aaatacttcg ccggaatacc aacatatatt gaccatggtt
     1621 caagattctt atttactaga ctcattgcac ctatagcagc accctctcca attgtaactt
     1681 cgggaaatat tatacaacct gccgctacga tcgagtgctt ttgaaatatc acagggcctc
     1741 cggttactcg cgtcaaattc ggatttacca ttggattggt cataaaatca ccgctataat
     1801 catcagtctg gctgtatact aaaacacgcc cagaaatagt aacaaaatct ttgatacaaa
     1861 taccgaattt cccatacaaa gctgtataag ctgaaatatg cacgtatgaa ccgattgata
     1921 tattccccga aagaatacaa aaatcatcta tcctaacatt attgcctaac tggataagtt
     1981 ccggtgagta gagactagct ttacggctaa ttaatacatt cttaccatac tttttcaatc
     2041 ctaattttat gatttcttcc tcattataaa atgacgtaat cataataccc cctcagcaat
     2101 acattaatat aacttttatt atctgatctt ggatatcttc agttaaatct tcatataaag
     2161 gtaaacacaa aattctggat gaaagatttc tcgaaatcgg cattttttgc tttggatgaa
     2221 taaaatgaag agtatctaaa gaaggataaa agtagcgtcg cggaaaaatt tcagcctgat
     2281 ttaaactttg ttttattcgt aaaaggattt tttcagattc gaataaaact ggaaaatagg
     2341 agtaattttg agtagagtgt ggtgctattt gttggagggt taacttatta ttatcaataa
     2401 atgcttccaa gtaacggtta taaatcacgt gtcttttttc gataatatct tggatgtcat
     2461 caagaacaca taatcccatt gcagcttgaa actcattcat tttagcatta attcctaaag
     2521 tggcgatttc atctggtccg ggaataccaa aatttattaa tagccttgcc ttttttgcca
     2581 tatcatcgtc tttaagaaca atcgctcccc cctcaatagt atgaaaaatc tttgtagcat
     2641 gaaaactaaa tatagaaacg tccccgaatt cactaatact cttattgtag tattttactc
     2701 caaaagcgtg tgccgcatca tatattactt ttagtttata agaattagct atttcttcta
     2761 tttttccaac attgcaaaca ttgccaaaaa catgaacgcc taaaatagct gatgtattat
     2821 tattaatatt tttctcgatt tgtataggat caatattaaa agtatctggt tcaatgtcag
     2881 caaaaatagg gatcagtttt tcccagacta aactactagt cgtggctatg aaagtaaaag
     2941 gcgtggttat tacttcacct tttaattcta ataagtgata agcaatttgt aaagctagcg
     3001 tcccattagc tgtcaatatt acatgaggaa cacctaagta atcttccaga cgtctttcta
     3061 gattctgtac taattcccca ttgttggtta accaaccgga tttaaaaatc ttattaatat
     3121 attctaaata cttttgcttt tggggtaaat atgttttcgt tacgttaatc atcgttattt
     3181 tctcttgtta gtatcaatac ttagaacttc tttccacatt ctaacataat tttcttcatt
     3241 gaaatggttt ttgattattt tataattatt ttcagctatt agcggcgaga tataagccag
     3301 ctcacagaag cttacatttt caagatctga atatggaaaa atgacaacat taattttttt
     3361 gaagtattcg tacggagtag tctcttttct gatatatact tttttcccaa gaaaaagtaa
     3421 agaaagtatg tttccaagtc cttgctgacg cgatgagcca aagattgcaa catttacttt
     3481 gagtaataat tttgaataat ctttcggttg gataacctct aataacggcg aaaatttttc
     3541 tccaaaggca tcttgaccat attgaataac cgagtttata tattcttttg tcccaccata
     3601 tgataaaggg caaaatattt taaaatgtcg gtctgaaaaa cgttttaggt gttcaagaac
     3661 tccaatatga tcattagatg gatcaccgga attcccaacc tggatggtaa tctcttcatt
     3721 gactagatca ttacatctta tactttctag atactcaaag tcagttggta atggataaac
     3781 agcatattga taaattgctc ttgtgttata tcttgatttg gcgatacgat aatctccttc
     3841 cataaaagca attatgcgag gcaattttac aataactcgg cgacggatag attcataaat
     3901 ggctgcctta aatccttttt ttctagtctc aacccaatat agatcatcac cccataatat
     3961 ccagctagtt ttcggaatta aagaaggaaa aattaaccat attattggag taattccaac
     4021 gggtagaaag tgtatataaa ttatctgtga ccttaataat aaaggaatta gtttaagtat
     4081 atatttactt ttcgaatcaa tgtataaaat atttttaatt tgaaatgatg gtggatattc
     4141 atattgtttg ttatgagaag cccgataaac aaaaaaatgt ttacttatat caaaatgctt
     4201 atgtatgaaa ttaataaatt ggtaattata aagattttcg caagtaaaaa tatgaacggt
     4261 cagcataaaa taaaattact aaatcattaa tatttctcta aacgttaata gccttatata
     4321 attctatcag ttgccttccc tgtatctcgg tattatagat ttccaatacg gctttgcgcc
     4381 ctttctcgcc cattgttttc cgaagttcgg gattatcctg taaatattgt attttcattg
     4441 ccaagtcaat agcattatca cttttatgta ctaatccgca accagttgtt tcgataaggt
     4501 ctttctgtgg tccacaatca ctgactataa ccggccgagc aaagagcatg tactgaaata
     4561 ctttatttgc tatcccggat tcatgctgat cattttttaa aattggtgag agacatacgt
     4621 cactgtcggt aatatatgat ggcatttcac taatatcttt ccaacgatag tggattatct
     4681 gatctttcaa tgtgttgtcg gataaatact tattgaattc ttttttttca tttgtatcaa
     4741 tcggtcctat cagcaataat ttaatttttt gtcgcccttg ttggattaga atccgcaacg
     4801 cctcaaaaac tgtgaatatt ccgcgacgac gagcaatcac cccaaaatac aggagggtaa
     4861 aattgtcttc cttatcaagt actctccgaa ttttatagtt aagtaaaccc ttaacacaag
     4921 gtacgtttgg ataaacagtg atagcatcat ctttgagaaa tgtgtatttt ctaagtaatt
     4981 gtattcgata atattgactt aatacgataa ttcgatccac attttgaaaa tataccgctt
     5041 cgatatcttc ccaatcttgt ggtctgataa ttaatcgtcc cgtcaatgta ttcgcccaag
     5101 tatatgcttt caaagctgcc ggataattct catgaagatc aactaccacc tgaataccaa
     5161 tatagcgtcg agctttaata ccagatggaa ccatatacaa atcatgtata tgtaatacat
     5221 ctatatgatt atgaactaca aattttttta ctttacgttc ccaaaagttc ttgtaaagat
     5281 tcagagatgg atgctgaaat actctcaaac gtttaattaa acattttgaa gttggaattc
     5341 gttgtatcac aatccctctg tagttttcta ttttagtttg acttctggaa taaccataac
     5401 ataaaacata aacttcatgt ccaccttcta atagaataag agcttcattt tccactcgtt
     5461 cgtccggtgg aaattcctta tccagaatca ttcctattct catgataatc gctcacaaat
     5521 gtcagttatt attgttttca tattcttttt aaaattgaaa ttccagtgct ctggatgagt
     5581 caaaagacat atggtagtta agtgtgaacg aattgcctcc tggggcgtaa gcccatattt
     5641 ccaattaaaa ttatttgtta tagaactatc cgaaatataa gcatcgaata acatagtaat
     5701 ttctggatca taggcttcaa attggatatc caggtcggca cgatttccat attttaagat
     5761 agcatggtta ggaattccca atatacgatt acgcttatca ccatgggatg cgattgtctg
     5821 aataccgcca aattgcgttt tgaaacgaga aatttctttt tcaagcaaat taaaacaggt
     5881 tttcatcacg gtatcagtaa ctcttttagc atccttaatg ttatgctttt tacaataagt
     5941 ggctaaagtc tcataatgca atgaagcttc gaaaccatct ctccgcattt tataaaccag
     6001 tgcctcgctt accgttttcc atcggaaata gtaggttgcg tgaacgccta acgacttttc
     6061 gattgcgtac attttcgccg caccctcggt atcaatatct acgtcatgtc ttagaattaa
     6121 tactctttgt ccattttggc agtacttatg aaaccactca attaaggatg tgataatata
     6181 gccttctttt tgggcgaata ttaacaattc acggtatact cccagacgcg atttttggcg
     6241 gatttttctc acaatatcaa atgtcttaat tgacattata ccctcaatat cttgagtaac
     6301 ttttattctt ttcctcaagc ggaaagcgat tatccagcac cataccgatc ctcagtctaa
     6361 ggtcagcggt tttcatgtat aatactcctg cggcgtaaca acgggctggc gatatttttc
     6421 cgtctcgata aacattctct cccaaatcgc caatcctagc acaatccaca gaaaattgta
     6481 atgccagcgc aggcgcggat caggcggata attcagaatc cgctgaaggt attcgtaatt
     6541 gaaaattccc tgatgttgga tgaattcacg cgtcagcact ttttcagcca cttttttcaa
     6601 atccttgttg aattgatcgt aggggttgac ggcgaagccc cgttttttct tctgcaaaat
     6661 ttccggtggt aaatagggtg ccattgcttt ccggaaaaga tatttcgttt gtccgttctt
     6721 catttttaga gtggctggga tcgtaaaggc aaactcgacc aaatccttat caaggaacgg
     6781 cactcgcaat tccagcgaat gcgccatcga catccgatcc tcggtcagaa tataatcatt
     6841 cagcatttta gaatgaaatt cgacataggc aatcgcatcc agcggcggaa gatcgcgaca
     6901 cgccgcgaaa atcggatcga accaggtacg tgtcggcgcc agcgtcgttt gtaaaaattc
     6961 cggctggtag atttccttcg caaatctcat gtcataatcc cagcaattgc gcagaatcag
     7021 gtaacatttc tccagatcgc cagccgaaaa cagcatttgc aagccacgtc ggtattcgtc
     7081 ccacttgaga attttagttg aattctgcaa actgaaaagc agagtgccaa ttgaatccca
     7141 aagcgttttt tggcagaacc ccggacggtt ttgcaataat cctcgcatcg cccataaata
     7201 gcgataaata tcataaccaa cgaaaagctc gtcgccccct aaaccgccga gcacgacttt
     7261 caaactatgc gaaacaaatt ccgataaatg aaaaccctgc aggagattga tcttcggctc
     7321 ctcggcgtgc ctgattgttt ccggaaaata ctgtaatggt tccaacgcca gtgctgtagt
     7381 tgtatggttc gtgttaaatt gccgggcaat taccgccgca tcatcgaatt cgtcggttgc
     7441 ctcattgaag ccgagcgtga aagtattaat tgggcggatt ttttccgccg ccatcatcgc
     7501 cactaaggcc gacgaatcca ttcctccgga aagataaaca cccagcggca catcgctgat
     7561 tagctggcgt ctcacggctt gttgtaagtg aaaacgaatg ccgtcaatgg cttcggattc
     7621 agtcatactg aaattaagat ttaccggcaa ctgataatat ttcttgatgc tcacttgccc
     7681 gttttcgtaa atcaaataac tggcgggcgg cagacgataa atccctttga tcaatgtttc
     7741 atcactttga ttgtaacgta gattgacctg gcagtgcaga gcggaataat tcacctgacg
     7801 tcgcattgcc ggatgcacca gaatggcttt atattccgaa ctaaacaaca gcacttcgtt
     7861 ctgaaaccaa tagtggaggg gtttgactcc aaaatgatca cgcgccaaaa tgagtcgttc
     7921 gtttttaata tcatataacg caaaggcaaa gataccattg agatgcgcga gaaattccag
     7981 tccatattcc tcgtaaccgt gactcaaaat ctcgacgtcc gagttggtat aaaaatggtg
     8041 accgcgggtg attaaatcag ccttgaattc ctgaaaattg taaatctcac cgttcattat
     8101 gacgactacc gtgcggtctt cattataaat cggctggtcg ccagtttcta aatcaatgat
     8161 tgccagacgt cggtgtccca ggctgacatt accattatgg aaatagccga cattttccgg
     8221 accacgatgc tttaataaat cagtcatctc ggtgatcaac cggtggttat tttcattcgg
     8281 atcaactatt ccgcaaatcc cacacatgat taataatttt gtaatagatt tatggtaaaa
     8341 ttgtcattgg attaattctg tcaatagtca aataatcggc aatgatcatt tctaaattaa
     8401 taaacactcg ctaccacctg acatattttc tcgatatcat ctcttgagag ttctgcatac
     8461 attggtaatg ataaaaattg ggtggatagc tgttctgaat acggtaaatc gccttgttta
     8521 taatgcaaat agctatacgc cggctgtaaa tgcagaggta atggataatg aatgccgaaa
     8581 gctatttgat tggcttttaa agcctcaatt attttttgac gccgatctgt ggcgatagca
     8641 aaaatgtgat agacatgttt tttggccgga ttcatttccg gtaaggtgat tggcaggttt
     8701 tttaataacg aataataagt attggcaata ttgattcgtt gctcggtcca ttctggtagg
     8761 tgacggagtt tgacatccag aatagccgcc tgtaaagtat cgagacggta gttatatcct
     8821 tcagtctggt ggacgtattt ctcttttcgc ccatgatttg ccagaagttt ggctttttca
     8881 taaagcgttt cattgttagt acccaaagct ccggcatcac cgtaagcgcc cagatttttc
     8941 ccagggtaaa aactaaaggc ggcgacatct ccgaaagtgc cgactttctg gttttggtat
     9001 tctgctagat gggcttgagc acaatcttcg atgattttca aattatattt cctagcaaaa
     9061 ctggaaaccc gatccatttc gcagggatta ccatgcagat gaaccacaac gattgctttg
     9121 gtattaggcg taaccagttt ttcgaggctg ttgcaatcaa tgttaaacga aaccggatca
     9181 atatcggcaa aaacaggctt agcacccacc tgacgcacag tttcagccgt agcaatgaaa
     9241 gtcatagcgg gaacaatcac ttcatcgccg gaagttatac caagaatttc atacgccaga
     9301 tgcagagccg cagttccact cgctaagcct agcatatatt tcaggttctg ggctgtagca
     9361 aaattctttt cgaaggtggc tacctcagga ccattaatga atttgtaact atgtataacg
     9421 ttgaaaatag ctgaatctat ttcctgtcga atactgagat attgcgtttc tagatccata
     9481 aaaggaatca tataaaacct ctttgattat aaatccctga ttttgcgggc gggattgccg
     9541 acgtaaattc ctgggtgtaa aatatctttg gttacgaccg ctccagctcc aattatgacc
     9601 ctgtcagtaa tttttaccgg taatacggtt gcattagtac cgatggcgac gtgattacca
     9661 ataatggttg gtttccataa ctcccgatta ccaccagccg gtttacctga tttaaatgtg
     9721 tcgttaacaa atttagctcc atgagaaatg aaacaatcat cgccgatcgt tactaattcg
     9781 caaataaacg aatgggactg aattttacag cgccgaccaa ttttgacacc tttctggatt
     9841 tcaacgaaag gaccaatgaa agtgttatca ccaatctcgc attcatagag attacacggc
     9901 tcaataataa ccacatttgt cccacattta atatcacgga tcccgatttt ttttataatt
     9961 ggttcagcca tatttacttc aatttacttt gcgaataaat gtcttcaatg attcgaacga
    10021 ctttcagtcc ttcaatggcg ttagtggcaa tcgtgtcttg tccgtttaat acattgacga
    10081 cattttcgta aactttatcg tgattcgaca tcgagccttg gtaaaatcca tagtcattgg
    10141 ctttattgac cggcgtaacg ttctggaaag aataatcttc gatattttga tattcaagtg
    10201 cgtttaaata ttgaccgccg attttcactg tccccttact accgaacacg gttatggaac
    10261 cttccatatt tttttggtag ctatttacag tgtaattaat agtcccgatg gctccattgc
    10321 aaaattcgat taaaacggcg ccggtatctt caaattcaat gttgggatgt agataattct
    10381 gaagaagcac ttttacattc ttaaaatcgc cgaataacca gactaataga tcaatgaaat
    10441 gactgaattg ggtataaagg gtgccaccgt ctttatcgag ccgtcctttc cagtcggatt
    10501 gcaaataata atcctcattt ctattccaga aacagtttaa ctgaacataa aaaatcttac
    10561 ctaaccgatt tccttcgatg acttttttta attccacaac tggaggatta tagcgatttt
    10621 gtttgacaat gaaaagacgt cggttggttt gttcggctgt ataaatcata ttttcacagt
    10681 cgcgcactga aagcgccata ggtttctcgc agagaacatg ttttccggct ttcagacttt
    10741 ttatagaatg ttcggcatgc agatagttgg gcgtacacac cgaaacgaca tccacatccg
    10801 gttcattttt tagaagggtg tcaatgtcaa aataaggacg gcagttatac ttctccgata
    10861 atgttaacgc cgcgctttct ttaatatcgc agacggcgct taactcgccc agacgatgaa
    10921 tttgttcggc gtggcgttgc gcaattcgac cacatcctat taacgcaaat tttattgtct
    10981 tcaattatca aatccctttt ggtttcaatg tttatgagca aaatttaact ggaggtttca
    11041 ggatattcgc aatttaccga taagaaataa ggcatataat aagttttgcc ggtaatggca
    11101 acttcagaca tattgccggc attggtttta tccttaatta actctttcaa aaagcacatc
    11161 acgcatatat tgaaaattag tgaaaattgg ctgagaatca attgaaaaga taaaatgatt
    11221 caattcagga ttttctgtaa tatttgtcaa aaatttcagc cagttgccgc gtaaggttgc
    11281 ggcgggaaaa agtcattaca tattccttat ctatttcaat agccggttca cccttttccc
    11341 atttttcata taaactcaat aaataattag cagaatccgc gatgttatca tattcaaaca
    11401 ttctaccggc attggctctg cgtaggattt tcgcggcatc gccctgagtc ggtccataag
    11461 ccaaaatcgg tttgtacgta gcgagataat catacatttt ataattaatt actccgcctt
    11521 cgggagtcac atggataaac agtaaaagca catctgaaga aacaagatat tgcttgactt
    11581 cgtcagccgg catatagggc aagaaattga tcatctcgaa aacggaatat tgccgcaatt
    11641 tctgttttac aaattcgggg aaattgccaa taaactgaat tataatattt tctctgatat
    11701 tttcattttg aatcaagact tcaatggctt tacaaaatgc ctgaatatcc tgttcaatac
    11761 tgatgcttcc ag
//
LOCUS       DAOSMP010000056        11344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_5714_length_11344_cov_20.303924, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000056 DAOSMP010000000
VERSION     DAOSMP010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 11344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11344
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5714_length_11344_cov_20.303924"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="PKX09_08475"
     CDS             complement(<1..1058)
                     /locus_tag="PKX09_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17896.1"
                     /translation="MLRRIEFKIVLLGLLAWVLLLNPVNLLAIGDKYAGEFLKFGLGV
                     REMALGGAVVSDPTAVAAHYWNPATLTDNTCFSGNFMHTEEFAGAVALDHFALALPGK
                     GEYSFGAGYLRIGVDDIADTRNALLDLGTDGLGPGDEGYLGPDADGTEGNGRLDPGER
                     LDYGKFGSFGASENAVFLSGAKILQPKLKVGGTLKMLYRSLGDDKAYGIGFDIGVLYQ
                     PAPKINLAAAFNDLTTTFMLWNDGEREIVAPSLRIGGSVHHHFKKLPLAIEPLFGADI
                     LFEGKQNTADISIGGTVVRARIGLEVIYKECIALRAGRDDLGNPQIGLGLNTSVGSLD
                     YGLGLGGSYAEFGSSHRIA"
     gene            complement(1116..3731)
                     /gene="mutS"
                     /locus_tag="PKX09_08480"
     CDS             complement(1116..3731)
                     /gene="mutS"
                     /locus_tag="PKX09_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOU17897.1"
                     /translation="MTKSTEKIDETTPVMRQYNAVKAQYPDALVLFRMGDFYETFRED
                     AKQAAKILNITLTKRANGKAAAVPLAGFPYHALESYLHKLLKAGLKVAICEQVEDPKL
                     AKGVVKRAVVEIITPGTAISDRFLEKEKNNYLVSVYINGENAGLAALDISTGEFYLSE
                     IPTARITEAVLDLHPAEIICPESIQKEIQTLFTNQVLRITPLEDWIFTFETAYQTLIE
                     HFKTDSLKGFGCQSAVHGITAAGPILHYLRQNYQTNLQHIYSIRNLATDETVLIDDFT
                     RRNLEIFQTMQSGDRKGTLMDVIDQTVSPMGVRLLTKWLIRPLRDPAAINQRLDGVEF
                     LYNNTKIRHQLREMLAHCGDLERLISRLSTNRASAREIVALKNTLKLIPGIRDLLPNQ
                     LAFSEINSLLNPLPELVELLERAIKDEDLPLSIHEGGFIKDGYSAELDEYRHIARHGK
                     EWIARLQETERQKLGIPSLKVGYNKVFGYYIEVTRTHAEKIPPEYIRKQTLVNNERFV
                     TPELKEYEEKLLNAEERINQIEFELFQDLRTKILQFIEAIQKNAGALAQIDVLAGLAE
                     LAEKNRYCRPVINDGTAIDIKAGRHPVVEHLLPPGEKFIANDLHIDNLSEQILIITGP
                     NMAGKSTYLRQIGLIVLMAQIGSFVPADSATIGVVDKIFTRVGASDNLAAGESTFLME
                     MHETANILNNVTPHSLVLLDEIGRGTSTYDGMAIAWSVTEYLHNQPNVAAKTLFATHY
                     HELTELEKILPRLKNYNVAVREYGDRVIFLRKIVPGGCDKSYGIHVAQMAGVPPEVIG
                     RAREILTNLSPEEKVLPTNREKFRHLPEKDANQLGLFGDDLKLKKMLAEVDIDNLTPL
                     EALQKLAEIKKSLGG"
     gene            complement(3902..6094)
                     /gene="feoB"
                     /locus_tag="PKX09_08485"
     CDS             complement(3902..6094)
                     /gene="feoB"
                     /locus_tag="PKX09_08485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOU17898.1"
                     /translation="MNFPPHRHRHRRGQRQISPDSSQKVIRIALAGNPNSGKTSLFNA
                     ITGQHQHIGNYPGVTVEKKYSLFHYKDDVIEIIDLPGTYSLTAYSLEEIVARDFVIKE
                     KPDVIIDVLDATNLERQLYLLLQFQELGVPLVGALNMSDECEKKGIDIDHERLSSILG
                     IPLVKTVGTRGYGIDCLLDTAIRVAKGKLRADQRHLNYGEDVENAHDQIIKVLETDPD
                     FSHKYPVHWLAIKLLENDRDAIQKVKSEHTNAETVLGESERIRRQLENHFHDDIISIL
                     AERRYAYINGAIREAVRIDRTRPAIDWTERIDRVILNRFLGIPIFLLIMFLIYQATFG
                     LGNPLAGLISNSFANLADFVNLHLPDNRFRDFLTNGLIAGVGGVLVFLPLVVLLMLGL
                     SLLEDTGYMARAAFVMDKFFHLFGLHGRSFIPLMLATGCAVPGVMAARSLANRRDRII
                     TIMVTPLMMCSAKTPVIAMLAAAFFPEKAGLIFWGIWFLSWFMALIIALIFRKTFFKG
                     DQSPFVMELPPYRWPSFRSVMIHMWQRTLAYLRKAGTIILFAAIIVWFALHNPTNPGS
                     HSTDASQFNVNNNQGSETVNQGQQLYHSYAGQIGRGLEPILKWAGFDWKIGVSLIAGL
                     AAKEVIISTLGMLYGIPEGAQEATEQSKMTTVSYQISRDPNYSPLSAFSLMIFVMLYT
                     PCLATLAVVRKELGHIKWALFQAVYTVTIAFIASVAIFQIGTFLQHIF"
     gene            complement(6091..6348)
                     /locus_tag="PKX09_08490"
     CDS             complement(6091..6348)
                     /locus_tag="PKX09_08490"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HOU17899.1"
                     /translation="MSTNSTTLVARSLAEVPAGKCFRIKKVHGHGEIRQRLIDLGFIK
                     NEVGTVIREALLKDPIEVELKGTHFSLRRSEARLIEVEVID"
     gene            complement(6351..7124)
                     /locus_tag="PKX09_08495"
     CDS             complement(6351..7124)
                     /locus_tag="PKX09_08495"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOU17900.1"
                     /translation="MNEKQSHGPTISQAQEDYLKIILELINSRRVARIKEIAQRKNVS
                     MPTVTEAMRKMAQDGLINYSAHEFIDLTSEGEQAAHQMTARHKFLRLFLEDILNIPSE
                     TADTEACLLEHHLSPITLERMILLYQFLVNCPHHDSNLIEDFRQCLSAEKLADKTSCR
                     DCFVKIAFPHTVTNPFAVHSLLANLSEGQKGTVTMLGGNTAMRHKLIEQGLVPGATVQ
                     MERTGNKSQPFVISVNGLQIKLDIEQANLIEVAISSGEI"
     gene            7419..7700
                     /locus_tag="PKX09_08500"
     CDS             7419..7700
                     /locus_tag="PKX09_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17901.1"
                     /translation="MSDNFNNSGKKGSAQPYDSKINEQCPKCSGNIVYRKSTYFGNKV
                     YYKVCNNCGWYITLPKEVWKDIISASLKPAKATPSESAEQDEQIEAIVS"
     gene            7792..8454
                     /locus_tag="PKX09_08505"
     CDS             7792..8454
                     /locus_tag="PKX09_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17902.1"
                     /translation="MVLTGSKSRDVILIGAAFKAEINPLIEKLSQASFSPTGIDFDYI
                     LTGSGLERVRCCLAEKVVCQDYLAIINIGTAGVLNDTCPLMSIFYPHRFCTLSNDHLT
                     YLTLPNDFLESSHFLSTGWKSGTLFSSREPVTSAAKRWAILQNSQADVVDMEAFAYAD
                     FCQRHKIPFACLKVITDRADAEVKQNFNANLAESAQLLAQNALIFVDFLLNKLDIKTE
                     NG"
     gene            8447..9310
                     /locus_tag="PKX09_08510"
     CDS             8447..9310
                     /locus_tag="PKX09_08510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU17903.1"
                     /translation="MDDPQKISVIIPTYNRETCLERAIVSVLSQTYRNFELIVIDDGS
                     TDNTSRIIHKYKNKIRYFSQLHSGVSTARNLGLEKSEGTWVAFLDSDDYWLPRKLERQ
                     MQCLAENPQWLVMQTDEKWIRNGVVVNPTKKHQKYSGWIFAQCLPLCIVSPSAVIVHQ
                     KVFNDVGVFDESLPVCEDYDLWLRVSLKYEIGLLPEKLIIKTGGHSDQLSRKYWGIDR
                     YRVLALEKILNAELDQQQTELVLDEIIKKLTILSKGRTKHPELPNIYADKLSDYQKKK
                     EDFLANQKNCH"
     gene            9285..10235
                     /locus_tag="PKX09_08515"
     CDS             9285..10235
                     /locus_tag="PKX09_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17904.1"
                     /translation="MQIKKIAIEQIIPSQSQERLFVEEINPDCNKLNESLFLQPVFLH
                     HTCDGFIILYGFNYISDIYKCGGKNIQAFVIPKSQSLFSALNIVADFIRRSRPLWPIE
                     IARILQLFNQQQFSNAKKSAFFKKFTEKDLIRTLEEQYLALIGLNPLLHKFLMEKKAP
                     LKTWFLTAECPPVIRDLILNLITECQPSLSTFEEISRNLLEILRREDIAPEQLTAEIN
                     QVIIEKAGEIQPSQKLEIFRSKIQERRFPQMTIHRERLNNFLQTIQLPSAVEIEFNPN
                     FEERGYRLKAEIRTPNDLRELTEFLNSVAQSDLKHLLEIL"
     gene            10255..>11344
                     /locus_tag="PKX09_08520"
     CDS             10255..>11344
                     /locus_tag="PKX09_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17905.1"
                     /translation="MNLPADHLIKKIFVTAECARDEIVEQIFTAYPKQKKIAIESEKS
                     LIETIPQEDPKKILLVTHSKGEVVKDCPGTAAPYLCCRYQVINQTLNCPLNCTYCILQ
                     YYLNQPATIIYTDYQKIIAEIEQKIASQSHRLLRFGTGELGDSLALTASQIFAIKLIG
                     YFATQKNALLELKTKTDRIGLILDQPHNGHTVISWSLNPLVIRNSEERLAPPPTRRLK
                     AAAEAARRGFLIGFHFDPIIDIPDWESQYLDLIEELYHYVDPARIAWISLGSLRFPPS
                     MKAKIIARYPYSRLPYGELIRGQDQKMRYPRPRRTEIYRSIYRKLTEIREPPFVYFCM
                     ESPTVWQDVCGFAPQSNAHLDYLFAESLW"
BASE COUNT         3178 a         2466 c         2555 g         3145 t
ORIGIN      
        1 gctatgcggt gcgaggaacc gaattcggcg tacgagccgc ccagccccag cccataatct
       61 aaactgccga cggaagtatt gagtccgaga ccaatttgag gattgccaag atcatcgcgg
      121 ccagcccgca aagctataca ttctttataa attacttcca gtccgatccg cgcccgtaca
      181 accgtaccgc cgatagatat atccgccgta ttttgtttac cctcgaacaa aatatcagca
      241 ccaaatagag gttcaattgc cagcggtaat tttttaaaat gatgatgaac cgagccgcca
      301 atccgcaaac ttggcgcgac gatttcgcgc tcaccgtcgt tccagagcat aaatgttgtc
      361 gttaagtcat tgaacgccgc cgctagattg attttaggcg ccggctgata aagtacgccg
      421 atatcaaagc caattccata ggctttatca tcgcccagcg aacgatagag catcttgaga
      481 gtgccgccaa cttttaattt aggttgcaaa attttggctc cggaaagaaa aaccgcgttt
      541 tcgctggcgc cgaacgaacc gaatttaccg tagtcaaggc gctcgccggg atcgagtcta
      601 ccattgcctt ccgtaccgtc ggcatccggt cccaggtagc cttcatcacc cggtcctaaa
      661 ccatcagttc caagatcgag taaggcgttg cgcgtatcgg caatatcatc aaccccaatc
      721 cgcaaatagc cggcgccgaa ggaatattcg cccttaccgg gtaatgccag cgcaaaatga
      781 tcgagcgcaa ccgcaccggc gaattcttcg gtatgcataa aattgcccga gaaacaagtg
      841 ttgtcagtca aggtagctgg attccagtaa tgcgccgcca cggcggtcgg atcagataca
      901 accgcacctc ctaacgccat ttcccgcaca cccaagccga atttgaggaa ttctccggca
      961 tatttatcgc cgatggctaa gagatttacc ggatttaaca ataaaaccca agccagcaga
     1021 ccgagcagga ctattttaaa ttcaattctt ctcagcataa aattgcaatt aatacggccg
     1081 taatgcgcgg aatgttccct gaattgcaat tgcattcaac cacccaagct ctttttgatt
     1141 tcagcgagtt tttgcaaagc ctccagcggc gtgaggttat caatatcaac ttctgccagc
     1201 atttttttca atttgaggtc gtcaccgaac aacccgagct ggttggcatc tttttccggc
     1261 aaatggcgga atttctcgcg attggtaggt aaaacctttt cctcaggact caaattagtt
     1321 aaaatttctc gggcgcggcc gatgacttcc ggcggaacgc cagccatttg agcgacgtga
     1381 atgccgtaac ttttatcgca accgccggga acaattttac gcaggaaaat gacccgatcg
     1441 ccgtattccc gtacggctac attatagttt ttcaggcgtg gtaagatttt ttccaattca
     1501 gtcaattcgt ggtaatgagt ggcaaataat gtcttggcgg cgacgttggg ttgattgtgt
     1561 aaatactcgg taaccgacca ggcgattgcc ataccgtcat aagtcgaggt gccgcggcca
     1621 atttcgtcca gcaataccag tgaatgcggc gtcacattat tgagaatatt ggcggtctcg
     1681 tgcatttcca tcagaaaagt ggattcaccg gcggctaaat tatcactggc accgacgcga
     1741 gtgaagattt tgtcaactac gccaatcgtg gcggaatcgg ctggcacaaa gctaccgatt
     1801 tgcgccatta aaacgattag cccaatctgg cgcaggtaag tagatttgcc agccatattt
     1861 ggtccggtga taattaaaat ttgttcgctg agattgtcaa tgtggaggtc gttggcgatg
     1921 aatttctcgc ccggcggtag cagatgctct acaactggat gacgtccggc tttgatatca
     1981 atcgctgtcc cgtcattgat gaccgggcga cagtaacgat ttttttccgc cagttctgcc
     2041 agtccggcta aaacatcaat ttgggctaac gcgccggcgt ttttttggat tgcttcgata
     2101 aactgtaaaa tcttggtgcg caaatcctgg aataactcga attcgatctg attgatgcgc
     2161 tcctcagcgt tcaacagttt ttcctcatat tctttcaatt caggagtgac aaagcgctca
     2221 ttattgacca gtgtttgttt acggatatat tccggaggga ttttttcggc gtgcgtacgg
     2281 gtgacttcaa tgtaatagcc gaagactttg ttgtagccga cttttagcga gggaataccg
     2341 agtttctggc gttcggtttc ctgcaagcga gcgatccact ctttgccgtg gcgggcgata
     2401 tgacgatatt catctaattc cgccgaatag ccgtctttaa taaatccgcc ttcgtggatg
     2461 cttagaggta aatcctcgtc cttaatggcg cgctctaaga gttcgaccaa ttccggtagt
     2521 ggattgagaa ggctgttaat ttccgaaaaa gccagttgat tcggaagaag gtcacggata
     2581 ccgggaatga gttttaaggt attttttaaa gcgacaattt cccgagcgga agcgcggttg
     2641 gtgctaaggc gcgagatcag gcgctctaag tcaccacaat gcgctaacat ttcgcgcaat
     2701 tggtggcgga ttttggtatt attgtataaa aattcgaccc cgtcgagtcg ttggtttata
     2761 gcggctggat cacgcaatgg tcgaatcaac catttggtta acaaacgcac acccatggga
     2821 ctgacagtct ggtcaatgac gtccattagc gtaccttttc tatcgccgct ttgcatagtc
     2881 tggaaaattt ccagattacg ccgggtgaaa tcgtcaatca ggactgtctc gtcggtcgcc
     2941 agattgcgaa tagaatatat atgctggaga ttggtctggt aattttggcg caaatagtgc
     3001 aaaatcgggc cggcggcggt aatgccgtgc accgcagatt gacaaccaaa acctttgagc
     3061 gaatcggttt taaagtgttc gattagagtt tgataagcag tttcgaaggt gaaaatccaa
     3121 tcctcgagcg gagtgatgcg caaaacctga ttggtgaaca gagtttggat ttctttttgg
     3181 attgattctg gacagatgat ttcagccggg tggagatcga gcacggcttc cgttattctg
     3241 gcagtcggga tttcgctcag ataaaattca ccagtggaga tgtccagagc ggcaagtccg
     3301 gcattttctc cattaatata aacggagacg aggtaattgt tcttttcttt ttcgaggaag
     3361 cgatcggaaa tagccgtgcc gggagtgatg atttcgacca cagcgcgctt gactacccct
     3421 ttagccagtt tgggatcctc gacctgctca cagatagcga ctttcaggcc ggctttcagt
     3481 aatttgtgta aatatgactc aagggcgtgg taaggaaatc ctgccagcgg aacagccgcg
     3541 gctttgccat tggcgcgctt agttagagta atattgagta ttttggcggc ttgtttagcg
     3601 tcttcgcgga aagtctcgta gaaatcaccc atgcggaaaa gcaccagcgc gtccggatat
     3661 tgagccttga cggcgttata ttgacgcata actggagtgg tttcgtcaat tttttctgta
     3721 gatttagtca tcgcatttca tttccgattg aaatatacag aaagtaacgc aatgttgaaa
     3781 acgggaaaat cagagtttat ctcactaaaa atcgccggtt tttccgaaca aagttgaata
     3841 attaagagta agctgaaaga atttttttca agacagcttg tttttaccga aaacattgat
     3901 atcaaaatat gtgttgcaga aatgtgccta tctggaaaat agcaacagat gcaatgaagg
     3961 caatcgttac tgtataaacg gcttgaaaaa gagcccattt tatgtggcct aattctttcc
     4021 tgaccaccgc caaagtcgct aggcaagggg tatagagcat tacgaaaatc attaaactga
     4081 aagccgatag aggtgaataa tttggatccc ggctaatttg ataacttacg gtggtcattt
     4141 tagattgttc agttgcttcc tgggctcctt ctgggatgcc gtaaagcatt ccgagagtgg
     4201 aaataataac ttcctttgcc gccagaccgg cgattaaact gactccgatt ttccagtcaa
     4261 atccagccca ttttagaatt ggttcgagtc cgcgaccaat ttgcccggcg taactatgat
     4321 ataattgctg accctgatta actgtttcac taccctgatt attatttaca ttaaattggc
     4381 tggcatcggt agagtgtgaa cccgggtttg tcggattatg aagtgcgaac cagacaataa
     4441 tggcggcaaa taaaataatc gtcccggctt ttcgtaggta ggctaaggtt ctctgccaca
     4501 tgtgaatcat cacgctccga aaagaaggcc agcggtaagg aggcagttcc atcacgaaag
     4561 gactttgatc gcccttaaag aaagttttgc ggaaaataag agcaatgatt agagccataa
     4621 accagctaag aaaccagatg ccccagaaaa ttagtccggc tttttccggg aaaaaagccg
     4681 ccgccagcat ggcgattacc ggggttttag cactacacat catcaatggt gttaccataa
     4741 tggtgataat tcggtcgcgt cgattagcca acgaacgagc ggccattacc ccgggaactg
     4801 cacagccggt cgctaacata agcggaataa aactccgacc gtgcaaaccg aataaatgga
     4861 aaaatttatc catcacgaaa gccgctcgag ccatataacc ggtatcttcc agcagagaaa
     4921 gtcccaacat taataataca actaatggca gaaaaaccag aacgcctccc acacctgcta
     4981 ttaaaccgtt tgtcaaaaaa tcacgaaacc ggttatctgg taaatgaagg ttaacgaaat
     5041 ctgccaggtt agcgaagcta ttcgatatca aaccggctaa gggatttccc aatccgaaag
     5101 ttgcttggta tatcaaaaac ataatcagca gaaaaatcgg gatccccaga aaccgattga
     5161 gtataacccg atcaattcgt tcagtccaat caattgccgg ccgagttcga tcaatccgca
     5221 ctgcctcgcg gatagcgcca ttgatgtagg cgtaacgtct ttcagctaag atcgagatga
     5281 tatcgtcgtg gaaatgattt tcgagttgac gtcgaatgcg ttcgctttcg cctaaaacag
     5341 tttcagcatt agtatgttcg cttttgacct tttgaatggc atcgcggtca ttttccagca
     5401 gtttgatagc cagccagtgg accgggtatt tatgagaaaa atccggatcg gtttccagca
     5461 ccttgataat ctgatcgtgg gcattttcaa catcttcgcc gtagtttaga tggcgctgat
     5521 cagcccggag cttgcctttg gcaactcgaa tagcggtatc caaaagacaa tctataccat
     5581 agccgcgggt accaactgtt ttaacgagtg gaatccccag aattgacgaa agtcgttcat
     5641 ggtcaatatc tatacccttt ttttcacatt cgtcagacat atttagagca ccaactaagg
     5701 gcacaccgag ttcctgaaat tgtaaaagca ggtacagttg tcgttccaag ttagtcgcgt
     5761 ccagcacatc aatgatgaca tccggttttt ctttaatgac gaaatcgcgc gcaacgattt
     5821 cttccaacga gtaggccgtc aaactgtagg tgccgggcag atcaataatt tcgattacat
     5881 cgtctttata gtggaaaagg ctgtattttt tctcaacggt aacccccgga taattcccga
     5941 tgtgttgatg ctggccggtt atggcattga aaagtgaagt tttacctgaa ttcggattgc
     6001 cggctaatgc aattcggatt actttctggg aactgtctgg tgaaatttgg cgctgaccgc
     6061 ggcgatggcg gtgtcggtgt gggggaaaat tcaatctatg acctcgacct cgatcaaacg
     6121 ggcttcactg cgccgtagtg aaaagtgagt gcccttgagc tcgacctcga tcggatcttt
     6181 tagcaaagct tcacggataa ccgttccaac ctcattttta atgaaaccaa ggtcaatcag
     6241 acgttgtctg atttcaccgt gaccatggac tttttttatt ctgaaacatt tgccggcggg
     6301 aacttcagct aggctccggg ctacaagagt agtcgaattt gtggacatta ttaaatttct
     6361 cctgaagata ttgcgacttc gataaggtta gcctgttcta tatcgagctt tatttgaaga
     6421 ccgttgactg aaataacaaa aggctggctc ttattaccgg ttctttccat ttgaaccgta
     6481 gcgccgggga cgagcccttg ttcaattaat ttatgtcgca tagctgtatt cccaccgagc
     6541 atcgttaccg tccctttctg tccttccgat agattggcta atagcgaatg aactgcaaaa
     6601 ggattagtaa cggtatgggg aaatgcgatt ttgacaaaac aatcacgaca actggtttta
     6661 tccgcaagct tttccgcaga cagacactgc ctaaaatctt cgatgaggtt gctatcgtga
     6721 tgaggacagt tcactaaaaa ctgatagagt aaaatcatcc tctctaaggt tattggacta
     6781 aggtgatgct cgaggagaca ggcttcggtg tcggcagttt cagatgggat gttcagtata
     6841 tcctcaagga aaagacgaag aaatttatgc cgggcggtca tctggtgagc ggcttgttca
     6901 ccctcggagg taaggtcaat aaattcatgc gcggagtaat ttatcaagcc gtcctgcgcc
     6961 attttccgca tagcttcggt cacggtcggc atactaacgt ttttccgctg ggcaatttct
     7021 ttaatgcggg cgacgcggcg ggaattaatc agctcgagga taattttgag ataatcttcc
     7081 tgcgcttggg agatggtagg tccgtgagat tgtttttcat tcatactgcc taatttaatt
     7141 aggttaacct aactacacaa tactttttgg agaaaatatt ctattggaaa aaaataaatt
     7201 gactactatt cgtttctgtg aacaaaatga attatagctc gtaaaagggg cgaatcagta
     7261 attttttgtt agtttagaaa ttaaatgctt gacaattacc attagaaata cgaatattta
     7321 gaccagtaga gaataagaat aaaaaattaa ttttttactg atttttagta gtaaatgtca
     7381 aatgtccgaa gaaaccaaat ttcaaaggtt caatcactat gagtgataat tttaacaaca
     7441 gtgggaaaaa gggaagcgcc caaccttacg acagcaagat taatgagcaa tgccctaaat
     7501 gttccggcaa catcgtctat cggaaaagca cttactttgg caataaggtt tattacaaag
     7561 tgtgtaataa ttgcggctgg tatataacct tacccaaaga agtctggaag gatattatca
     7621 gtgccagttt aaaaccggct aaggctactc cgagtgaatc tgccgaacaa gatgaacaga
     7681 ttgaggcgat tgtttcctaa tttggtctga ttccccaatt aatttttaaa atattcccaa
     7741 tattatgtgg aaaatttcca aaattgacca caaggcgtta atattgtatc aatggtccta
     7801 actggttcaa aatcgcgaga tgtaattctg ataggtgccg cttttaaagc cgagattaac
     7861 ccattaatcg aaaaactcag ccaggcttcc tttagtccaa cggggataga ttttgattat
     7921 atcctaactg gttctggctt ggaaagagta aggtgctgtt tggcggaaaa agtcgtttgt
     7981 caagattacc tagctatcat taatatcggt accgctggtg tcctcaacga tacttgtccg
     8041 ctaatgagta tcttttatcc gcatcggttt tgcacattga gcaacgacca tttgacctat
     8101 cttacactgc caaatgattt cctcgaatct agtcattttc tgtcaactgg ctggaaaagt
     8161 ggaacgctat tttcgagtag ggagccggta acttcagccg cgaagcgctg ggcaatttta
     8221 cagaattctc aggctgatgt tgtagatatg gaagccttcg cctatgccga tttttgccag
     8281 cggcataaaa ttccttttgc ctgcctgaaa gtcattacgg atcgggcaga tgccgaggtg
     8341 aagcaaaatt tcaatgccaa tcttgccgag tcagctcaat tgctggcgca aaatgcacta
     8401 atttttgttg atttcttgct gaataaactt gatattaaga ctgaaaatgg atgacccgca
     8461 gaaaatttct gtcataatcc caacttataa tcgggaaacc tgcctggaac gcgccattgt
     8521 ttcggttttg agtcagactt accgcaattt tgaattaatt gttattgacg acggtagtac
     8581 ggataatacc agccgcataa ttcataagta taaaaataaa attcgttatt tttcccaatt
     8641 gcattcagga gtgtccactg ctcgtaatct tggactggaa aaatccgaag gcacctgggt
     8701 agcgtttctc gattctgatg attattggtt gccgcgtaaa cttgagcgtc agatgcaatg
     8761 tttggcggaa aatccccagt ggttggtgat gcaaaccgac gagaaatgga ttcggaatgg
     8821 ggtcgtagtc aatccgacaa agaagcatca aaaatattcc ggttggattt ttgcccaatg
     8881 tctgccgctt tgtatcgtca gcccttccgc ggttatcgtt catcagaagg tttttaatga
     8941 tgtcggagta tttgatgaaa gtttaccggt ttgcgaagat tatgatctct ggttgcgagt
     9001 ttccttgaaa tatgaaattg gattactacc ggaaaagtta atcattaaaa ctggtggtca
     9061 ttccgaccag ctttcgcgca aatactgggg cattgatcgt tatcgagtgc tggcattgga
     9121 gaagatttta aacgccgaac tcgaccaaca acaaactgaa ttggtactcg atgaaatcat
     9181 taaaaaactt acgatcttga gcaaaggacg cactaaacat cccgagttgc ctaatattta
     9241 tgccgacaaa ctatctgatt accaaaagaa aaaggaagat tttcttgcaa atcaaaaaaa
     9301 ttgccattga acaaataatc ccgtcacaat ctcaggaaag gttgtttgtg gaggaaatta
     9361 atccggattg taataaactg aacgaatcgc ttttccttca gccggtattt ttacaccaca
     9421 cttgcgatgg tttcattatc ctttatggat ttaattatat ttccgacatt tataaatgtg
     9481 ggggaaaaaa cattcaggcg tttgttattc ctaaaagtca atcgcttttt tcagccttga
     9541 atatagtggc ggattttatt cggcgctcgc gtccgctttg gccaatcgaa atcgcgcgga
     9601 ttttacagct atttaatcaa cagcaatttt ccaatgccaa gaaatcggca ttttttaaaa
     9661 aatttaccga gaaagatttg atccgtactt tggaagaaca atatttagca ttgatcgggc
     9721 tcaatccact tttgcataag tttttaatgg aaaaaaaagc gccgctgaaa acctggtttt
     9781 taacggctga gtgcccgccg gtgattcgag atttgatttt gaatttaatt actgaatgcc
     9841 agccatcgct gagtactttt gaagaaatta gccggaattt gctggaaatt ctccgccgcg
     9901 aggacattgc tcctgaacaa ttgacagcag aaataaacca agttataata gaaaaagccg
     9961 gggaaattca accatcacaa aaacttgaga tattccgtag taaaattcag gaacgccgtt
    10021 tcccacagat gactatccat cgggagcggt taaataattt tctgcaaaca attcaattgc
    10081 catcggcagt cgagattgaa tttaacccga attttgaaga acgaggttac cgacttaagg
    10141 cggaaattcg cacgccaaac gatctgcggg aacttactga atttttgaat agtgtcgctc
    10201 aatccgatct gaagcattta ctggaaatac tgtaaatagt gaactgcaat tccgatgaac
    10261 ctgccggctg atcacttgat aaagaaaata ttcgttacgg cggaatgtgc tcgagatgaa
    10321 atagtcgagc aaatttttac tgcttatcct aaacaaaaaa agatagcaat agaatcggaa
    10381 aaatctttaa ttgaaacgat tccgcaggaa gaccccaaaa aaatattact ggtaactcat
    10441 tctaagggcg aagtagttaa ggattgtccg ggtacagccg cgccgtatct ctgctgtcgc
    10501 tatcaggtaa ttaatcaaac cttaaattgc ccgttgaatt gcacttattg tattttgcaa
    10561 tattacctga atcagccggc aacaattatt tataccgatt accaaaagat aattgccgag
    10621 attgaacaaa aaattgccag tcagtctcat cgtttattgc gcttcgggac cggcgaactc
    10681 ggcgatagtt tagctttgac cgcctcccaa atttttgcca ttaagttgat aggatacttt
    10741 gccactcaaa agaatgcttt gctcgagctt aaaaccaaaa ccgatcgaat cggtttaata
    10801 ttagatcagc cgcataatgg tcacacggtc atttcctggt cgctaaatcc attagtcata
    10861 agaaatagtg aagagcgctt agcgcctccg cctacaaggc gcctaaaagc cgccgccgaa
    10921 gccgcacggc gaggtttcct gattggtttt cattttgatc cgatcattga tattccggat
    10981 tgggaaagtc aatatctgga tttaatcgaa gaattatacc attatgttga tcccgcccgg
    11041 atcgcctgga taagtctcgg ttccttacgt tttccaccgt caatgaaagc caagataatc
    11101 gcgcgttacc cgtattcacg cctaccttat ggtgaactaa ttcgtggtca agatcaaaag
    11161 atgcgctatc ctcgtccacg gcgaaccgaa atataccgtt ccatttaccg caaactgacc
    11221 gagattcgcg agccaccatt tgtttatttt tgtatggaga gtccgactgt ctggcaagac
    11281 gtctgcggct ttgcgccgca atcgaatgct catctggatt atttatttgc ggaaagtctc
    11341 tggc
//
LOCUS       DAOSMP010000057        10732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_6133_length_10732_cov_19.474665, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000057 DAOSMP010000000
VERSION     DAOSMP010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 10732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10732
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6133_length_10732_cov_19.474665"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PKX09_08525"
     CDS             complement(<1..525)
                     /locus_tag="PKX09_08525"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOU17906.1"
                     /translation="MYLFGNLKAGFLNAWLGVLLIFLASWTILATDKKARRHLSNTSW
                     CSPREKKLVWSGTFLLFVILVFSIFVPLQLNTNWFYIGTAIFLVGYICLVISYFNYIK
                     TAGDKSKTKGFYNISRNPIYLFTSVALLGISIASASLIMLILVIIHFIISHFVILAEE
                     RHFLHIYKNTYKKYM"
     gene            complement(603..1817)
                     /locus_tag="PKX09_08530"
     CDS             complement(603..1817)
                     /locus_tag="PKX09_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOU17907.1"
                     /translation="MSLSFAENLQNALEEFQTGTQRFEEINRHLAKNLAWKKEAYEHL
                     KNLLLHMVKIGASDINLGGPRTNDKVWYRVYGNLEPSQEVPTYTNDEVAAIALSILSD
                     QQKTILFNERNIDFAFAFSTDQSQKPYRFRGDIYYERNNLAINFRHIAEKIFPIESLE
                     IPEPIIKRLNLQHEKSGLFLVTGVTGSGKSTTLDSITDMNNHNNKAHIVIIGKPIEYV
                     HKSDKSLITHREVGDDVLSFRNGIIEALRQDPNIIIVGEMRDPATISAVLEATDSGHK
                     IFSTLHTSSTVESIHRIIAEFPSDEQERIRFRLADTLKVVISQKLVPNKEGKRTLAKE
                     ILSVDYSVQAAIRNNNITEIFQMITEGKNKGMFTLQQDLYRLVRNGTITTETALNYCN
                     NKKIMQQLLGFS"
     assembly_gap    2056..2155
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2550..5408
                     /locus_tag="PKX09_08535"
     CDS             2550..5408
                     /locus_tag="PKX09_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOU17908.1"
                     /translation="MKISLRLALSLVLISGALLLADSRVQKLPNGVIIELAEEPAISS
                     RLIKIQVCDDDIIHVQATPESNFSERPSLIVEQAYANRLPESVNWTLRENEDTVILAT
                     AKLLVKISKTEGTVAFYKADGELILQERASGSKIFTAAEVMGEQTYHIQQFFDSPANE
                     AFYGLGQHQNGLMNYKGQSVDLWQYNIVVAVPFLISNRNYGILWDNNSRTKFGDIRDF
                     ESISIATLKLYDKKGKPGGLSAEYFKDDGFKKLFTTRREPRIEHEFIDVNDPFPAGFD
                     QVASVRWSGTIESAQTGIHKFRLYCCGYTKMWLNDSLVVDNWRQNWLPWTHFPEIFME
                     AGKRYSIKIEWIHQGGYIGLKYLTPSPEEYANSLSLYSEVADQIDYYFIYGNSLDEVI
                     HGYRKITGQAPLMPKWAMGLWQCRQRYQTQDEVLSVVREFRKRQIPLDNIVQDWFYWE
                     EDKWGDHEFDPARFPDPDGMVKELHEKLNAHIMISVWPKFYVGTKNYQQFLEKGWLYM
                     RNVEKNQRDWVGPGYVSTFYDPYRPEARDLYWQQINEKLFSKGFDAWWLDATEPDIHS
                     NLSPEETRLRMHPTAIGSAARYLNTYSLVHCKGIYENQRRTNPDQRVFILTRSAFAGQ
                     QRFAAATWSGDVAARWYDLKTQIPAGLNFCLSGIPYWTTDVGGFAVESRFVNATGENL
                     EEWRELMTRWFQFATFCPLFRVHGEYPYREIYHVAPEDHPAYQAMLAYDHLRYRLMPY
                     IYSLAGMVTHNDYTIMRALIMDFSQDTKVQNVGDQFMFGPALLINPVTEYKARTRSVY
                     LPAGTGWYDLKTGKYFNGGRTIIAEAPYSDIPIFVKEGSIIPYGPEIQYADEKSADPI
                     ILYVYTGRDAEFELYEDEGVNYNYEKGQFATIPLVYSEKTQTLTIGKRQGEFPGMLKE
                     RTFQIIRVSPDKMSGLGFTLEPDKIVKYDGQEITIKIK"
     gene            5426..7858
                     /gene="galB"
                     /locus_tag="PKX09_08540"
     CDS             5426..7858
                     /gene="galB"
                     /locus_tag="PKX09_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase GalB"
                     /protein_id="HOU17909.1"
                     /translation="MKGIGLKIMLVLVASLLITQCSDSRKMVRQIDNFGQSWRFNLGD
                     ISDAERPEYDDSNWRTLDLPHDWSIEGEFSEEHPATPGGGALPGGIGWYRKTFTVSEG
                     NKDKSVFIDFDGVYQNGEVWINGNSLGKRPYGYSSFRYELTPYLNYGDENNIIAVKVD
                     NSQQPNSRWYSGSGIYRNVWLVVTDKVFVDHWGTFVTTPEVSSEAAKINIKTNVRNQT
                     GLDQEVILSTIIYDADGKLIARANTSQKIPKDTVIEIEQELSVKKPKLWSDERPYLYQ
                     AVTQISGAEKVWDDYSTSFGVRYFKFDPEKGFFLNGKPVKIKGVCNHHDLGCLGAAIN
                     TRAIERQLELLKSMGCNGIRTSHNPPAPELLDLCDRMGFIILDEAFDVWRVAKMKYDY
                     HLYFDEWHARDMADLVRRDCNHPSVFIWSIGNEVMEQWDKSNPSGMEIAQELANITRK
                     LDPTRPITAGCNDQNPENPLIKSNALDLIGYNYAHEKFADFPKTYPGKCFIATETHSV
                     IATRGSYDMPSDSIRVWPTRWDIPFTGGNPDFSCSAYDNCHVPWGSTHAETWSIVKKY
                     DFISGLYIWTGFDYLGEPTPYSWPARSSYFGIIDLAGFPKDSYYFYQSEWTEQPVLHI
                     FPHWNWQKGELIDVWAYTNYDEVELFLNDQSLGKKQKSDGVFQLVWRIEYTPGTLKAV
                     AHKKGSADLTKVIKTAGEPAGILLEPDRDVIKADGKDLSFITVKVVDSEGTTVPLAEN
                     LINFEVEGPGFIAGVDNGSQISHEPFKANYRKAFHGLCLAVIQSKGMPGQITVKANSE
                     GLKPAVARIRVK"
     gene            8016..9503
                     /locus_tag="PKX09_08545"
     CDS             8016..9503
                     /locus_tag="PKX09_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOU17910.1"
                     /translation="MGILIFNRKKYPTLSIGIILLVFFRSVLGQDYIPPDSTDMRNIT
                     SIELAQEMIPGWNVGNSLEAIGGETAWGNPQITQRLIDSVKAAGFNAIRIPVAWSKFS
                     DESTFTIEESWLERVEEVVWYVLSSGMYAIINEHWDNGWIQPTYADSAYVKNRLAAMW
                     KQIAIYFRDYDDHLLFAGTNEVMKEGNWGEPTKEYYTVQNMYNQVFVNTVRATGGRNY
                     YRHLLVQGYRTDINCTVKYFVIPQDVFPNRLMVEVHYYDPYNFTLNEDDKIIQWGKYA
                     TDASRTETWANESWADGQFNKMKTNFIDKGYAVILGEYCATSRLNLGSAELNAQHAEY
                     RRYYINYVTYSMVYHGLVPFYWDNGFTGNHGSGIFNRADGTQAYPNLVKAIMDGVNLT
                     VFPVGIKDEEKPKTAREFVLNQNYPNPFNSQTKISYYLPRTGKVTVKVYDLNSCEVAT
                     LVRQELQKPGYYEVSFDASKLSSGLYFYQLSSEHFTDTRKMLLLK"
     gene            9679..>10732
                     /locus_tag="PKX09_08550"
     CDS             9679..>10732
                     /locus_tag="PKX09_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase"
                     /protein_id="HOU17911.1"
                     /translation="MRRIWRIIIILGFGIRILVGAEPPPGFFLDDFQSKKATVPPFKE
                     VTLTQSRSTVTVEIDIGDTLTKVPQYIYGHNANVYMTQMVNEPTLIGYIKQLAPQILR
                     YPGGNLSNLFFWNNQPGQLPEDVPDPLYGGVEKPYREEFWFGRNDTAITLSVDNYYKM
                     LEMTNCTGIICVNYSYARYGKGENPVATAAKYAADWVRYDNGRTKYWEIGNENFGNWQ
                     AGYKINTADNKDGQPEIITGELYGQHFKVFADSMRAAARSIGAEIKLGAGLLESAQDW
                     HTATEKTWNSGFFKQAGDAADFFVIHSYYTPWNENSSAGTILNSAIPVTQEMMTFMQE
                     TARINEVKMKPVGLTEW"
BASE COUNT         3002 a         2099 c         2459 g         3072 t
ORIGIN      
        1 catatatttt ttataagtat ttttgtaaat atgaagaaaa tggcgttcct ctgctaagat
       61 tacaaaatgg gaaatgataa aatggataat tactaaaatc agcataatga gcgaggctga
      121 ggcaatactt attccgagaa gtgcgacaga ggtaaaaaga tagatgggat ttcgggagat
      181 attataaaat cccttggtct tagatttatc accggcagtt ttgatataat taaaatagct
      241 gatcaccaaa caaatatatc ccacaagaaa tatggctgtt ccaatgtaaa accaattggt
      301 attgagttga agcggaacaa atatcgagaa tacaagaatt acaaataata agaatgttcc
      361 ggaccaaact aacttttttt cccggggact gcaccaggaa gtgttcgata gatgtctgcg
      421 tgctttcttg tcagtggcta agatagtcca actcgccagg aatattagca aaactcccag
      481 ccaagcattc aaaaagccgg ctttaagatt tccaaataaa tacatttttt accctttttt
      541 ttcttactta tggctgaatt ctttgcaaag agcatagcgc ggagaaattc atcaataact
      601 ttttatgaaa aacccagtaa ctgctgcatt atctttttgt tgttacagta gttcagggcg
      661 gtttccgtgg tgatggtacc gttcctcacc agccggtata aatcttgttg aagcgtgaac
      721 atgcctttat ttttaccttc ggtaatcatc tggaagattt cggtaatgtt attgtttcta
      781 atggcggctt gaacggaata atccacggag agaatttctt tggcgagggt tcttttacct
      841 tctttattgg gcaccaattt ttgcgaaatt accactttta atgtatcagc gaggcggaat
      901 ctaatccttt cttgttcgtc cgacgggaat tcggcaataa tccggtggat actttcgacc
      961 gtcgaactgg tgtggagggt agagaaaatc ttgtgaccgc tatcagtcgc ttccagaacc
     1021 gccgatatgg tagccggatc gcgcatttca ccgacgataa taatgttagg atcctgcctc
     1081 agcgcttcga taatgccatt gcgaaaactc aaaacgtcat cgcctacttc gcgatgagtg
     1141 atcaatgact tatcggattt atggacatat tcgatcggct taccaatgat gacaatatgg
     1201 gctttgttat tatgattatt catatcggtt atagaatcca gagtggtgct tttgcccgaa
     1261 ccggttacgc cggtaactaa gaataagccc gatttttcat gctggaggtt gagccttttg
     1321 ataattggtt cgggaatctc taaactttct atagggaata ttttttcagc aatgtgtcgg
     1381 aaattaattg ccaggttatt tctctcgtag taaatgtcgc cgcgaaaccg gtatggtttt
     1441 tggctttggt cggtagaaaa ggcaaaggca aaatcaatgt ttctttcgtt aaagagaatc
     1501 gttttctgtt ggtcggataa aatgcttaat gcgattgcgg caacctcgtc attagtgtag
     1561 gtaggtacct cctggctcgg ctctaaattt ccataaaccc ggtaccaaac cttgtcattg
     1621 gttctgggac cgccgaggtt tatatcagaa gcgccgattt ttaccatgtg aagcaataga
     1681 ttttttagat gttcataggc ttcttttttc caggcgagat tttttgcaag gtgacggttg
     1741 atttcctcaa accgctgagt tccggtctga aattcttcca atgcgttttg caggttctcg
     1801 gcaaatgaca gactcataag gttccttctt gattttttta ggtgaaaatt tctttagtgt
     1861 tcatcaggct ggttaatcgc atttctgtat attttaattt aaaattttca ttttccaatg
     1921 aagaaaatag ggatgaaaaa agttacggaa aatacagttt tttatttcgc atgactgcat
     1981 gactgcataa atacatgagt ttttaaagtt ataaagttgt gacgaattat atattcttat
     2041 aaacacttaa aagtannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntttta
     2161 aagttataaa gttgtgacga attatatatt cttataaaca cttaaaagta cttagtagca
     2221 cttattagga cttaatttct taccgccctt agcctcgccc ttgaggcaat ctttttaaat
     2281 ggtgaaaatt ataaaatgct aaaggcgcag cttgactcgc catttcggcg ttgacttgaa
     2341 accagtaact tgtttcctta gccttatttc tcgtgcctct gttgttaaag ttcctttcaa
     2401 tcatttgcca atttgcttta gttcaggcgc gaacatgatt agtaaattat tctcggaacg
     2461 attaaaattt tgccatcagc catattaatt gattatattt ggagcatgga tatacggctt
     2521 ttgaaatttt acaaataaga ggcgaacaaa tgaaaattag cttaaggctg gctctatcgc
     2581 tggtgttaat ttccggcgca cttttgcttg ccgattcccg agtacaaaaa ctgcccaatg
     2641 gtgtcattat tgagctggcg gaagaacctg caatctcgtc cagactgata aagattcagg
     2701 tctgcgacga tgacattatt cacgtccagg ctacacccga gtccaatttt tctgagcgtc
     2761 ccagtctgat agtggagcag gcttatgcca atcggttgcc tgaatcggtt aattggacat
     2821 tacgggaaaa tgaagatacc gtcattctag caactgccaa actgctggtg aaaatatcga
     2881 aaactgaagg tacggttgct ttctataaag cagacggcga actgatttta caggagagag
     2941 ctagcggtag caagattttt actgcggcgg aagtgatggg cgaacagact tatcatattc
     3001 aacagttttt cgattcgcct gccaatgaag ccttttacgg attgggacaa caccagaacg
     3061 gattgatgaa ttataaagga cagtcggttg atctctggca atataatatc gtggtagccg
     3121 tgccgtttct gatttccaac cgcaattatg gtattttgtg ggacaataat tcccgcacca
     3181 agttcggtga tattcgcgac tttgaatcaa tatcaatagc cacattaaaa ttatatgaca
     3241 agaaaggcaa accgggtggt ctatctgccg aatattttaa agatgacgga tttaaaaaac
     3301 tatttacaac tcgccgcgaa cctcgtattg aacacgagtt cattgacgtc aacgatccat
     3361 tcccagctgg attcgaccaa gtagcgagtg tgcgctggag cgggacaatc gaatccgccc
     3421 aaaccggcat tcataagttt cgactctatt gttgcggcta tactaaaatg tggctgaatg
     3481 actctttagt ggtggataat tggcgtcaga attggttgcc ctggacgcat tttccggaaa
     3541 tttttatgga agccgggaag cgctattcaa ttaaaattga atggattcat caaggtggat
     3601 atattggcct caagtatctg actcccagcc cagaggagta tgctaatagc ctttcgcttt
     3661 attcggaggt tgctgaccag attgactatt actttattta tggtaacagt ttagatgaag
     3721 tgattcacgg ctaccgcaaa attactgggc aagcgccgct gatgccaaaa tgggcgatgg
     3781 gtttgtggca atgtcgccag cgttatcaga ctcaggacga ggtgctttcg gttgtgcggg
     3841 aatttcgcaa gcgccagatt ccgctggaca atattgttca ggattggttc tattgggaag
     3901 aggataagtg gggcgaccac gaatttgacc cagctcgctt tcccgatcca gacggtatgg
     3961 tcaaagaact gcatgaaaaa ttaaatgctc acattatgat ttcggtctgg ccaaagtttt
     4021 acgtcgggac taaaaattac cagcaatttc tggaaaaggg ctggctatat atgcgtaatg
     4081 tcgaaaagaa ccagagagat tgggtcggac ccggctacgt ttccacattt tacgatcctt
     4141 accgtccgga agccagagat ttatactggc aacagatcaa tgaaaaatta ttcagtaaag
     4201 gatttgacgc ctggtggctg gatgctaccg aaccggatat tcattccaat ttgtcgccgg
     4261 aagaaacgcg cttgcggatg catccaactg ccatcggtag cgccgctcgc tatttgaata
     4321 cttattcgtt agttcattgc aagggaattt atgaaaatca gcgccgaact aatcccgatc
     4381 aacgggtttt tattctaact cgttcggctt tcgccggaca acagcggttt gccgccgcga
     4441 cctggagcgg ggatgttgcc gcccgctggt atgatttgaa aactcaaatt ccagctggtt
     4501 tgaatttttg tctatccgga attccctatt ggacaacgga tgtcggtggc tttgcggtcg
     4561 aatctcgctt tgtcaacgcc accggcgaaa atctggaaga atggcgcgag ctaatgaccc
     4621 gctggtttca gtttgccacc ttttgtccgc ttttccgggt tcatggagaa tatccctacc
     4681 gtgaaattta tcacgttgcg ccagaagatc accccgccta tcaggcaatg ttagcctacg
     4741 atcacttgcg ctatcgctta atgccctata tttattcgct ggctggtatg gtaacgcata
     4801 acgattatac gattatgcga gcgctcataa tggatttcag tcaggataca aaagttcaaa
     4861 atgtcggcga tcaatttatg ttcggtccgg ctttgctcat taatccggtg acggaatata
     4921 aagcccgcac acgctcggtc tatttaccgg ctggtactgg ttggtatgat ttgaaaaccg
     4981 ggaaatattt caacggcggt cggacgatta tagccgaggc cccttatagc gatataccga
     5041 ttttcgtgaa agaaggttca attattcctt acggaccgga aattcagtac gccgatgaaa
     5101 aatccgctga tccgattatt ttgtatgtct ataccggtcg ggatgccgag tttgaacttt
     5161 acgaggacga aggagtcaat tataattatg aaaaagggca atttgccaca attccattgg
     5221 tttattctga gaaaactcaa acattgacga ttggaaagcg tcagggtgaa tttccgggaa
     5281 tgttgaagga aagaacattt caaattatcc gagttagccc ggataaaatg agcggacttg
     5341 gctttactct cgaaccggat aaaatagtaa aatatgatgg ccaggaaata acgataaaaa
     5401 tcaaataata tttttggggg tatttatgaa agggatagga ttaaaaataa tgttggtgct
     5461 tgttgcatca ctgctcatta ctcaatgctc tgatagccgc aagatggtca gacagattga
     5521 taattttggc caaagttggc gatttaattt gggagatata agtgatgccg aaagacccga
     5581 atatgacgat tccaattggc gtactttaga tttgccccat gattggagta tcgaaggtga
     5641 atttagcgag gagcatcccg caaccccggg tggtggtgct ttaccgggtg gaattggatg
     5701 gtatcgcaag acttttactg tttccgaggg aaataaagat aagagtgttt tcattgattt
     5761 tgacggcgtt taccaaaatg gcgaggtctg gataaatggc aactccttgg gaaaaagacc
     5821 gtacggctat agttcttttc gatacgaatt gacaccctat ctcaactatg gcgacgaaaa
     5881 taatattatc gctgtaaaag ttgataattc ccagcaacct aattcgcgct ggtattcggg
     5941 atccgggatt taccgaaacg tctggttggt agttaccgat aaggtattcg tagatcattg
     6001 gggtacattt gttaccacgc cggaggttag tagcgaagcc gccaaaatca atataaaaac
     6061 caacgttaga aatcagactg gtctcgacca ggaagttatc ctgagtacca tcatctatga
     6121 tgctgatggg aaattgattg ccagagccaa tacttcccaa aagataccaa aggataccgt
     6181 gattgaaatc gaacaggaat tatcagtgaa aaaacccaag ttgtggtcgg atgagcgtcc
     6241 ttatttatac caggctgtga ctcaaattag cggagcggaa aaagtctggg atgattattc
     6301 gacttctttt ggagttcgtt atttcaaatt cgatccggaa aagggatttt ttctgaatgg
     6361 taagccagtg aaaattaaag gcgtatgtaa tcatcacgat ctcggctgtc tgggtgccgc
     6421 aattaacacg cgtgcaatcg agcggcaact ggaattatta aagtctatgg gttgtaatgg
     6481 gattcgcact tcccacaatc cgccggcgcc ggaattgctg gatttgtgtg atcggatggg
     6541 ttttatcata ttagatgaag ctttcgatgt ttggcgagta gcgaaaatga aatacgatta
     6601 tcatctttat ttcgacgaat ggcatgcgcg cgatatggcc gatttagttc gacgcgattg
     6661 caatcacccc agcgttttta tctggagtat cggaaacgag gttatggaac agtgggataa
     6721 aagtaatccc agcggcatgg aaattgccca ggaattggct aatattacca gaaagctcga
     6781 tcccacgcgt cccatcacag ctggctgtaa cgatcagaat cccgaaaatc cactcattaa
     6841 atctaatgca ctggatttga ttggctataa ttatgcgcat gaaaaatttg ccgactttcc
     6901 gaaaacctac ccaggtaagt gttttattgc caccgaaact cactccgtta tagcgacgcg
     6961 tggcagttat gatatgccgt cagatagcat tcgcgtctgg ccgacgcgct gggatattcc
     7021 ttttactggc ggcaatccgg acttttcatg ctcggcttat gataattgtc acgtgccctg
     7081 gggttcgact catgccgaga cctggtcaat cgtgaaaaag tatgatttca tttcgggatt
     7141 gtatatctgg accggttttg attatctcgg cgagccgact ccttatagct ggccagcgcg
     7201 tagttcctat tttggaatta tcgatctggc ggggttccct aaagacagtt attattttta
     7261 ccagagtgaa tggactgagc aaccggtatt acacattttt ccgcactgga actggcagaa
     7321 aggcgaatta attgacgttt gggcttatac gaattatgat gaggtcgaac ttttcctcaa
     7381 tgatcaatcg ttgggtaaaa agcaaaaaag cgacggtgtt tttcaattgg tttggcgaat
     7441 agagtacaca cccggcaccc tgaaagctgt ggcacacaaa aaaggctctg ctgatttaac
     7501 caaagtaatt aagactgctg gtgaaccggc tggcatttta ctcgagcccg atcgcgatgt
     7561 tataaaagct gatggtaaag acctgtcatt tattacagta aaagtcgttg atagcgaagg
     7621 cacgaccgta ccgcttgccg aaaatctgat taattttgag gttgaaggac ccggctttat
     7681 cgccggtgtt gataacggca gtcagatcag ccacgaaccg tttaaagcca actataggaa
     7741 agcctttcat ggattgtgtt tagcggttat tcagtctaaa ggtatgcccg ggcaaattac
     7801 tgtgaaagct aattctgaag gcttaaagcc ggctgtcgcc agaattcggg taaaatgaaa
     7861 aaaatctagt ggcgcattga ggtgctattt aaaatttgaa atgatttagt gtaaatacat
     7921 ctcgaaccgg catttcaggc gggtccattt taaaaaacct agctgaaaac gccaatctga
     7981 tattaaaagt tatttctaat gaaaagggtt tcaaaatggg gattttaata tttaatcgca
     8041 aaaaataccc aactctttca attgggatta tcctgctggt attttttagg tcggttttgg
     8101 ggcaggatta tatcccaccc gactcaaccg atatgcgcaa tattacttcc attgaattgg
     8161 ctcaagaaat gattcccggc tggaatgtcg gaaattcact ggaagccatc ggtggcgaaa
     8221 ctgcttgggg caatccgcag ataactcaga ggttgattga ctccgtgaaa gccgccggct
     8281 tcaatgccat ccggattccc gttgcctgga gcaaattttc cgacgaatct acttttacca
     8341 tcgaagaatc ctggctggag cgagtcgaag aagtggtttg gtacgttttg agtagcggga
     8401 tgtatgccat tatcaacgaa cactgggaca atggctggat tcaacctact tatgccgatt
     8461 cggcatacgt aaaaaatcgg ttagcggcga tgtggaaaca gattgccatc tatttccgcg
     8521 actatgatga tcaccttctg tttgccggca ccaacgaagt aatgaaagag ggcaattggg
     8581 gcgagcctac caaagaatat tataccgtcc agaatatgta taatcaggta ttcgtcaata
     8641 ccgttcgtgc caccggcggg cgcaattatt atcgccatct attggttcag ggttatagga
     8701 ctgatattaa ttgcactgtc aaatattttg ttattcctca agacgtgttt cccaatcgtt
     8761 tgatggtgga agtgcattat tatgacccct ataatttcac cttaaatgaa gatgataaaa
     8821 ttattcaatg gggaaagtat gctaccgatg cctcgcgaac tgagacttgg gctaacgaat
     8881 cctgggcgga cggacagttc aataaaatga aaaccaattt tattgacaaa ggttatgcgg
     8941 taattttagg tgaatattgc gcgacgtcaa ggttgaatct cggcagtgcc gaactcaatg
     9001 cccagcatgc cgaatatcgg cgctattata ttaactacgt tacttactcg atggtctatc
     9061 acggtctggt gcccttttac tgggataatg ggtttaccgg caatcatggc tcgggtattt
     9121 ttaatcgggc cgacggcaca caggcttatc cgaatttggt gaaagccatt atggacggcg
     9181 tcaatttgac ggtgtttccg gtaggcatta aagatgaaga aaaaccgaaa accgctcgtg
     9241 aatttgtgct taaccaaaat tatcccaacc ccttcaattc gcagactaaa atttcctact
     9301 atttgccgcg caccggaaag gttaccgtca aagtctatga tttgaacagt tgtgaggtag
     9361 cgactctcgt acgacaagag ttacaaaagc ctggttatta tgaagtgtca tttgacgcct
     9421 cgaaattatc cagcggctta tatttttatc agctgtccag cgaacatttc actgatacca
     9481 gaaagatgct tttactcaaa tgagtccgct ctaaataatt cgagttgtct gcaaaatttt
     9541 tgatattgga atcattttgt ttgtataatt gattcgaatg gaattgatga cgccaccata
     9601 aagggatggg accacagaaa ttgaattgat tggttaaaaa gttacacaaa accgataatt
     9661 ttataggggg aaatcgagtt gcgaagaatc tggcgtatta taattatttt aggatttgga
     9721 atccggattt tagtcggagc agaacctccg ccgggatttt ttctggatga ttttcagtca
     9781 aagaaagcca ctgtaccgcc ttttaaggaa gtcacgttaa cgcagtcccg ttcaaccgtg
     9841 acggttgaaa ttgatattgg cgatacgctg accaaggttc cgcaatatat ttacggccat
     9901 aatgccaatg tctatatgac tcagatggtc aatgaaccca ccttgattgg ttacataaag
     9961 caacttgcgc cgcaaatcct gcgctatccg ggtggaaatt tgagcaattt atttttctgg
    10021 aataatcagc ctggccaact gccggaggat gtgccggatc cgctttatgg cggtgtcgaa
    10081 aaaccatatc gcgaagagtt ctggttcggg cggaacgata ccgcaattac gctttcggtt
    10141 gataattact ataagatgct agaaatgacg aattgcaccg gaattatttg tgtgaattac
    10201 agttatgctc gatatggcaa gggtgaaaat ccggtggcta ctgcggcaaa atatgcggct
    10261 gactgggtgc gctatgataa tggtcgaacg aagtactggg aaatcggcaa cgagaatttc
    10321 ggcaattggc aggctggata taaaataaat acagccgata ataaagacgg tcagccggaa
    10381 attattaccg gcgaattata cggacagcat ttcaaagttt ttgctgattc gatgcgtgcc
    10441 gccgctcgtt cgattggcgc ggaaatcaag ttgggagccg gtttgctcga atcggcgcag
    10501 gattggcata cagcgacgga aaagacctgg aattccggct tttttaagca agccggagac
    10561 gcggccgatt tctttgtcat tcattcttat tatacaccct ggaatgaaaa ttcttcagct
    10621 ggtacaattc taaattccgc cataccagta acgcaagaga tgatgacttt tatgcaagag
    10681 accgcccgaa taaatgaagt aaaaatgaaa ccggtgggct tgaccgagtg ga
//
LOCUS       DAOSMP010000058        10698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_6161_length_10698_cov_14.310533, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000058 DAOSMP010000000
VERSION     DAOSMP010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 10698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10698
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6161_length_10698_cov_14.310533"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1605
                     /locus_tag="PKX09_08555"
     CDS             <1..>1605
                     /locus_tag="PKX09_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17912.1"
                     /translation="DEQEVNYSYIVRTISTDPEMVDISSSGQVNVNIDMYGENSESDV
                     TFSEIKGIVEPQNIKDSGEINTASDARISTAQIASGGMTFSIENGINIGTTGIPHLVL
                     TFPEILTTAGTPVTIESDIPGGQTTINYDLSNCLIQPLSESVTADSVRQYITYKSLVT
                     TSSGEIAEYSLIDSIKFDINTSEMTFSSVTGYFDQEAIVANEVLELEDKTKLETAQIS
                     NGNLILTFVNNIGVFADVHFTINEIVHRTDGTPLERIIHLPNSSQPVIETIPLDEYNI
                     QLPFTDLTTNQKIHYTSRVSIPSDREMTITFGQEINVDVLLTEVEFASVSGYIDTVQV
                     DIEPSENEVNALPEEFDGINLKNVEMAIEFNTDIGVPVELNLFISSFNDEGESVRREI
                     HQVITENPRVVIPDAEELINIKPKKIVTSGYALVYGTGQVDTAQYVSGNISISVPFEM
                     EITEDAKMEFDPELVEEDIPEEIESAILYAEVENGFEIGGKLILLAAQDTLLFDIVQP
                     YGPDTLAVINVHPDSSFREVVELGEKQ"
     assembly_gap    1606..1705
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1706..2500
                     /locus_tag="PKX09_08560"
     CDS             <1706..2500
                     /locus_tag="PKX09_08560"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOU17913.1"
                     /translation="SDTDLDLQSVVTLGIAHGWQIDNVPYLSNYVDKFSVGIAVKLLA
                     GAGYSGFDDLTANVTTYKDRVILEGDLKGEYGMGGAGLAIDVGAATVINEKMTANLAL
                     NNVVGFLNWGIGEAGKAEYSFYADIPSSDFGDIDSIMDESLRKDTTYTVSGIRTNYPA
                     YMIMGFEYRVLQNLRLFVNYRQSFSNDMASTTTPAFSFAAEFLPANWLPIRLGIGVGG
                     NDKIKWGVGSGMIFSRYRLEWGFAQTGGFFNHGKGFAFCLDQSLVF"
     gene            2504..>3094
                     /gene="xth"
                     /locus_tag="PKX09_08565"
     CDS             2504..>3094
                     /gene="xth"
                     /locus_tag="PKX09_08565"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOU17914.1"
                     /translation="MLLTLISWNVNGIRAVYKKGFLDWVKATQPDILGIQETKAEESQ
                     LPPDLAQVSGYYSYFSSAVRKGYSGVALYSRLKPVSITQKFGIEKFDTEGRLLIADYD
                     QFVLFNVYFPNGQMSEERLRFKLEFYDAFLDYVDRVNVSGRNIIIGGDVNTAHTEIDL
                     AHPAANSNISGFLPEERAWLDKFLSHGYYDTFRLFNN"
     assembly_gap    3095..3194
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3195..3429
                     /locus_tag="PKX09_08570"
     CDS             <3195..3429
                     /locus_tag="PKX09_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOU17915.1"
                     /translation="LDKFLSHGYYDTFRLFNNEPGNYTWWSPFTNARERNVGWRLDYF
                     FVNKRFVANVRAAEILADIAGSDHCPVSLQIEI"
     gene            3598..5334
                     /locus_tag="PKX09_08575"
     CDS             3598..5334
                     /locus_tag="PKX09_08575"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOU17916.1"
                     /translation="MRFKTITSSLIVCVAITTVLTAQNLIGTNWKFQPGDDLAWADPQ
                     YDDASWNSIIAGRTWEVQGYENLDGFAWYRYSVIIPSTLKEKAVKHGGFILRLGRIDD
                     SDATYFNGELIGQMGGFPPNYVGAYGLDREYIIPVELIRWDQPNVIAIRVYDAGGGGG
                     LYYSAAELNVIGLSDQLKIEIALPQKDHVIKNCSEIVLPVIMRNESNESIEGILRLSI
                     NSDFGDKIYDKELKIELKNNSAKKYSYNLKKLQPGFYLGAVSFESLGWNKLHKFAIAV
                     DPEKVISPVDAQPDFDAFWEQAKAELATVDPQFKMIRQDELCTAKRDYYLVEMRSLGN
                     ILIRGWYSVPKNPGKYPAFLQVQGYGTNMQPTNLITEDGFISFGLNIRGHGNSRDEID
                     PGFPGYLQYHLENKYEYIYRGAYMDCVRAVDFLCSRPEVDTSRIIVDGQSQGGALSFA
                     TAALDDRIRLCVPGVPFLSDFPDYFKVAIWPGNEFKDYVNNHPETTWEEVFRTLSYFD
                     IKNLAPKVKAPVFMLVGLMDETCPPHINFAAYNNLQCPKEYIIYPYSGHGLPAENYQA
                     RMNWVRQQLNLK"
     gene            5442..6800
                     /locus_tag="PKX09_08580"
     CDS             5442..6800
                     /locus_tag="PKX09_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOU17917.1"
                     /translation="MNSVQRHNRHKCKIITVFWLIVILPILIQCEQNNESDQIGGSDL
                     TFVEEHGNLTVSGNRIIDQNGDPIVLRGMSLFWSQWMGKYYNYNCIKWLRDDWKCTVV
                     RAAMGIESGGYLTNPATEKAKIVTVIEACIDLGIYVIVDWHDHNAHEHQAQAIDFFQE
                     IATGYGTYPNVIYEVFNEPEQDNWKTVLKPYLNAVVDSIRAIDPDNLIVVGTPTWSQD
                     VDVASLNPLAQNNIAYALHFYAAYPYHKQTLRNKASTALNNGIALFVTEFGTVLNTGD
                     GPIDTAETRLWMNFMESNQISWCNWSIADKNETSAALKPGASANGGWSASEISESGLL
                     IRDYIIRGNTKQPNLVRKTRLIPAGFALAQNYPNPFNSSTQISFELPVSAGVNLGVYD
                     LRGNLICTLLNKVFPAGCYQTTWETNGAIGEIAGGVYILRFLADSEIGTTSISRKLSL
                     IK"
     gene            6844..>7495
                     /locus_tag="PKX09_08585"
     CDS             6844..>7495
                     /locus_tag="PKX09_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOU17918.1"
                     /translation="MRKTYLGGFLFFLILLNLFTNNNRLFSQEFNRDMETFQPLSYAE
                     ADKLAESILAQMTLDEKIALIGGDKYFFIRALPRLNLSEIYMSDATQGIHIRQSFNEI
                     DLSNYQPEKSTAFPCPISLAATWNRELAYQYARAIGEECKAANIGILLGPGMNIYRQS
                     QCGRNFEYFGEDPYLAARMIENYVQGVQSTGTVATLKHFLANNTEYYRKKSNSVVDE"
     assembly_gap    7496..7595
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7596..9134
                     /locus_tag="PKX09_08590"
     CDS             <7596..9134
                     /locus_tag="PKX09_08590"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOU17919.1"
                     /translation="LANNTEYYRKKSNSVVDERTLHEIYVPAFKAGIEAGAKAVMTSY
                     NLLNGEWCGESAYVINYLLRNQLGFQWLVMTDWWSVYNGEKLAKSGQNLEMPFTIALE
                     NVHQLLEDNKVQVSDIDRMAQSILRTCFAMKLYGRSRTAVEDDFYEAHEKVALQTARE
                     GIVLLKNENNILPVKEQVKNILVTGDYVKKLAVGGGSAYVEGYNNRQMISELQKVFGD
                     RIKYVKNPTVKQIKSAQLVLCNIGTSDSEGWDRPFELPEDQEQKVLKCVNNNPNTVVI
                     VTSGSGIRMTGWNEKARAIIYAWYPGQIGNQALAEILIGKVNPSGKLPITIEKEFKDS
                     PGYGYIPEGESLYFDWNGEGERAHPVYDVRYKEGIFIGYRWYESQKIEPLYPFGHGLS
                     FTSFSYSDLQVTPGKFSEVDTISVTFSVKNVGKCQGAEIVQLYVSDLESSLPRPAKEL
                     KGFQKIVLEPGEIKTVTLKLSQKDFAFWSPAIKNWTTEKGKFVILIGSSSQDIRLSKE
                     IELL"
     assembly_gap    9151..9250
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9251..>10698
                     /locus_tag="PKX09_08595"
     CDS             <9251..>10698
                     /locus_tag="PKX09_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOU17920.1"
                     /translation="TFSIGRAEAIGSTILHFTDYKDRRNHFAFYSVNAPIDGFDCDKE
                     SFMGLHNGPHEPVAVFEGKTRNSVAFGWSPVAVHQIEVKLNPGESKSFNFILGYVEYP
                     KSEKFTADGKINLTKVRQMQSRFESPESVTKAMNDLREFWDGLLSVFQVQTGEEKIDR
                     IVNIWNQYQCMVTFNMSRSASYFESGIGRGMGFRDSSQDLLGFVHQIPERARERLLDI
                     AATQLPNGGAYHQYQPLSKRGNYEVGDNFNDDPLWLICGVVAYLRETGDFPILDESVP
                     FDNDPNNQASLFEHLKRSFYHVVNNLGPHKLPLIGRADWNDCLNLNIFNTDPDQSFQT
                     GLLKTDGKTAESIMIAGAFVLYGQEFIELCEFLGKMDEATEARQHIDEMKKSIDRFGW
                     DGEWFLRAYDAFGRKVGSQENEEGKIFIESQGFCCMAEIGKEDGRMQKALDSVKKYLD
                     CEFGIVLQNPAYTKYYIELGEISTYIPGYKENA"
BASE COUNT         3030 a         1950 c         2333 g         2985 t
ORIGIN      
        1 gacgagcaag aagtcaacta ttcttatata gttcgaacca tttcaaccga tcccgaaatg
       61 gttgatattt cttcatctgg tcaagtcaat gtcaacatag atatgtatgg agaaaattca
      121 gaatctgatg taactttttc ggagatcaaa ggcattgtag agccgcaaaa catcaaagat
      181 agcggtgaaa tcaataccgc ttccgatgcc cgaatcagta cggcgcaaat tgcctccggt
      241 gggatgacct tttcaatcga aaacggcatt aacattggta caactggaat tccacacctt
      301 gttcttacat ttccagagat attaactact gcgggaacac cagtaacaat cgaatcagat
      361 attccaggcg gacagactac gattaattat gacttaagta attgtctaat ccaaccattg
      421 tccgaatctg taaccgctga cagtgttcgg cagtatatca cctataaaag tctggttacg
      481 acttcctcag gagaaatagc cgaatatagt ttaattgatt caatcaaatt tgatattaac
      541 acatctgaaa tgactttttc gagtgttacc ggatattttg accaggaagc gatcgttgct
      601 aatgaggtac ttgagctgga agataagact aaattagaga cagcccagat ctcgaatggt
      661 aatctcatct tgacttttgt taataacatc ggagtgtttg ccgatgttca ttttacaatt
      721 aatgaaattg tacatcgcac agatggtacg ccattagaac gtattattca cctgccaaac
      781 agttctcagc cggtgattga gaccattccg ctggatgaat ataatattca attgccgttt
      841 acggatttaa ccaccaacca aaaaatccac tacacctcgc gagtcagtat tccttccgat
      901 cgagaaatga caatcacttt tgggcaggaa atcaatgttg acgttctact gactgaggta
      961 gaatttgcaa gtgtcagcgg gtatattgat acggttcaag tggatattga accctccgaa
     1021 aatgaagtta acgcattacc ggaagaattc gacggaatca acttaaagaa tgtcgaaatg
     1081 gcaatagagt tcaataccga tattggtgtg ccggtcgaac tcaatttatt tatttcgtcc
     1141 tttaatgatg aaggtgaatc agttcgtcgg gaaatccatc aagtcattac cgagaatccc
     1201 cgggttgtta ttcccgatgc agaagaatta atcaacatca aaccgaagaa aatcgttaca
     1261 tccggttatg ccctggtcta cggtactggt caagttgata cagctcaata tgttagcggc
     1321 aatatatcca tttctgtacc gtttgaaatg gaaataactg aagatgccaa gatggaattt
     1381 gatccggaat tggtggaaga agacattcca gaagaaattg aaagcgccat tctttatgcc
     1441 gaagtagaga atggattcga gattggcggt aaactgatct tattagccgc tcaggataca
     1501 ctattattcg atatagttca gccttatggt ccggatactc tggcagtcat taatgttcac
     1561 cccgattcat catttcgaga agttgtagaa ttaggtgaaa agcaannnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnagcga tactgatctg gatttacaat cggtagttac
     1741 tctgggaata gctcacggat ggcagattga taatgtacct tatctcagta attatgttga
     1801 taaattttct gttggtattg ccgttaaact tttagctggg gcgggttatt ccggattcga
     1861 cgatctaacc gcgaatgtta cgacctataa agaccgagtt atcctggaag gcgatctcaa
     1921 aggcgaatac ggaatgggcg gtgccggtct ggcgattgat gtcggcgcgg cgacggtcat
     1981 taatgaaaaa atgactgcca atcttgcctt gaacaatgta gtcggttttt taaattgggg
     2041 aataggagaa gccgggaaag ccgaatattc tttttatgcc gatattccta gttcggattt
     2101 tggcgatata gattcaataa tggatgaatc tttaagaaaa gacactacct acacagtttc
     2161 cggtattagg acaaattacc cggcttatat gataatggga tttgaatata gagtattgca
     2221 aaatctgcgg ctgtttgtca attatcgcca gtctttcagc aatgatatgg catccacaac
     2281 aaccccggca ttttctttcg cggctgaatt tttaccggca aactggttgc ctataagact
     2341 tggaatcgga gttggaggca acgataaaat taaatggggc gtcggtagcg gaatgatctt
     2401 tagtcgctat cgtttggaat ggggttttgc acaaacgggc ggattcttta atcacggaaa
     2461 aggttttgcc ttctgtttgg atcaatcctt ggttttctaa gatatgcttc ttaccctaat
     2521 ttcatggaat gtcaatggaa ttcgggcagt atataaaaaa ggttttttag attgggtcaa
     2581 agctactcag ccagatatat taggaattca agaaacaaaa gctgaagaat cgcaattacc
     2641 gccggattta gcccaagtta gtggctatta ttcttatttt tcatcggcag ttagaaaagg
     2701 ttatagtgga gttgctttgt attctcgctt aaagccggtt tctattactc aaaaatttgg
     2761 aatcgagaaa ttcgatacgg aaggacgtct cctcattgcc gattatgatc aatttgtttt
     2821 atttaatgtt tattttccta atgggcaaat gtcggaggag cgactgcgtt ttaaactcga
     2881 attctatgac gcttttctcg attatgttga tcgggtaaac gtcagcggtc ggaatatcat
     2941 cataggtggc gatgtcaaca cggctcatac cgaaatagac ctagctcatc ctgcggcgaa
     3001 ttccaatatt tccggttttt tgcctgaaga aagagcttgg ctcgataaat ttctcagcca
     3061 cggttattat gatacttttc gattatttaa taatnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnngctcga taaatttctc agccacggtt attatgatac ttttcgatta
     3241 tttaataatg aaccgggtaa ttatacctgg tggagtcctt tcaccaacgc tagagagcgt
     3301 aatgttggat ggcgcctcga ctattttttc gtaaacaaac gattcgttgc gaatgttcgt
     3361 gccgccgaaa ttttagccga tatagcgggc tcagatcatt gtccggtttc gctccagatt
     3421 gagatttgag atagcttttt accattctgg taacaagcgt tataaaaatt aattaagaga
     3481 gttttaaaat ttagtctcgg attttatagc ctttattaat tatatttgaa acggtttatt
     3541 tacaaaatga tgtttatatg ttttcgataa aatttaacgt ttaaaaaggg ggataaaatg
     3601 cgttttaaaa caatcacatc gtcattaatc gtctgcgttg caattacgac ggttttaacg
     3661 gctcagaatt tgatagggac aaattggaaa tttcaacccg gcgatgattt agcctgggct
     3721 gatccgcaat atgatgacgc ttcctggaac tctatcattg ccggtagaac ctgggaagtg
     3781 caaggctatg aaaatttaga tggatttgcc tggtatagat acagtgtaat tattccatca
     3841 acgctgaagg agaaagccgt taaacatggc ggatttattc tgcgcctggg gcgtattgac
     3901 gattccgatg ctacctattt caatggtgaa ttaatcgggc agatgggtgg tttcccacca
     3961 aattatgtcg gtgcttatgg tctggatcgc gaatatatta tcccggtcga actgattcgc
     4021 tgggatcagc cgaatgtcat tgcaattcga gtttatgatg caggtggcgg tggtggattg
     4081 tactactcag ccgccgaact gaatgtgatt ggtctttccg atcagctaaa aatcgaaatt
     4141 gcccttcccc aaaaggatca cgtcattaag aattgctccg aaattgtact tccggtcatt
     4201 atgcgtaatg aaagtaatga gtcaattgaa ggtattctca ggctgtcgat caatagtgat
     4261 ttcggggata aaatctatga taaagaatta aagattgagt taaaaaataa ttctgctaaa
     4321 aaatattcct acaacttaaa gaaactgcaa cctgggtttt accttggggc agtaagtttc
     4381 gaaagtttag gctggaataa attgcataaa tttgcgatag cagttgatcc ggagaaggtg
     4441 atttcgcccg tcgatgctca gccggatttt gacgcttttt gggagcaagc caaagccgaa
     4501 ctggctacgg ttgacccaca gtttaagatg attcgccagg atgaattatg caccgctaag
     4561 cgcgattatt acctggttga aatgcgttcg ctgggaaata ttttaattcg cggctggtac
     4621 tcggttccga aaaaccctgg gaaatacccg gcttttcttc aagttcaggg ttatggtacg
     4681 aatatgcagc cgactaacct gattacagaa gatggtttta tcagttttgg tttgaatatt
     4741 cgtgggcacg gtaatagtcg ggatgagatc gatcccggat ttcctggcta tttgcaatat
     4801 catctggaga ataaatatga atatatttac cgcggcgctt atatggattg tgtgcgggcg
     4861 gtagattttc tgtgttcgcg accagaggtt gatacaagcc gaatcatcgt tgatggacag
     4921 agccagggcg gcgctctttc ctttgcgacc gccgccttag atgatcgtat tcgcctttgc
     4981 gtaccgggtg tgccgttttt atcagacttt cccgactatt ttaaagttgc catttggccc
     5041 ggtaacgagt tcaaagacta tgttaacaat catcccgaaa caacttggga agaagttttc
     5101 cgaacattga gttactttga tattaaaaac ttagcgccga aagtcaaggc accggttttt
     5161 atgctagtgg gtctgatgga tgaaacctgc ccgccgcata ttaactttgc ggcttataat
     5221 aatttacaat gcccaaaaga atacatcatt tatccctatt ccggacacgg tttgccggca
     5281 gaaaattacc aggcgcggat gaattgggtt cgccagcaac tgaatctgaa gtaatcaata
     5341 aatagcacta tcgttgtcaa tataaaggta atttcccggt tgtaaataaa tagggatgat
     5401 tgatgggtga aaaactcaat cgctaaaagg aatttttaaa aatgaattca gttcagagac
     5461 ataatcgtca taagtgtaaa attatcacag tgttttggtt aatagttata ttaccaattc
     5521 ttatccaatg tgagcagaat aatgaatccg atcaaatcgg tggttccgat ttaaccttcg
     5581 tagaagaaca tggaaacctt actgtcagcg gtaaccgcat tattgatcaa aatggtgatc
     5641 cgatagtgtt gcgcggaatg agtctcttct ggagccaatg gatgggaaaa tactacaatt
     5701 acaattgtat taaatggctt cgagatgact ggaaatgcac cgttgtccgg gcggcaatgg
     5761 gtatcgaatc gggtggttat ttgaccaatc ccgcaactga aaaggctaaa attgtgaccg
     5821 tcatcgaggc ttgtattgat ttaggaattt atgtcattgt ggattggcat gatcataatg
     5881 ctcatgaaca ccaggcgcag gcgattgatt tctttcaaga aattgctact ggttacggta
     5941 cctatcccaa cgtgatttat gaagtattta acgaacctga acaggacaat tggaagaccg
     6001 tactaaagcc ttatttgaac gccgtagttg attcaataag ggcgattgat cctgacaatt
     6061 tgattgtggt cggaactccg acttggtcac aagatgtaga cgttgcctcg ctgaatccgc
     6121 tcgcgcagaa caatatcgcc tatgctttgc atttttatgc ggcttatccg tatcataaac
     6181 aaaccttacg caataaggca tcaactgcat taaataatgg gatcgctctt tttgtgacgg
     6241 aatttggtac tgttctgaat accggcgatg gtccgattga taccgccgaa actcgcttat
     6301 ggatgaattt tatggaaagt aatcaaatca gctggtgcaa ttggtcaatt gccgataaaa
     6361 atgaaacctc agccgcctta aaaccgggcg cctcggctaa cggcggctgg tcggcatccg
     6421 aaatttccga atcaggattg ttaattaggg attatatcat cagaggtaat accaaacaac
     6481 ccaatctggt tagaaaaaca cgacttattc cagccggatt tgccttagct caaaattatc
     6541 caaatccctt taattcttct acccagataa gcttcgaatt accagttagc gccggggtta
     6601 atcttggtgt ttacgattta cgcggtaatt tgatctgcac tttgctaaat aaagtgtttc
     6661 ccgccggatg ctaccaaacc acctgggaaa cgaacggtgc gattggcgaa attgccggtg
     6721 gagtatatat attaagattt ttagccgatt ccgaaattgg aacgacttca atttcccgaa
     6781 aattatcact tataaaatga gactaagaga ataaattgaa ccaaattaat tacaggagtt
     6841 ataatgcgca aaacctactt aggtggtttt ttattttttc taattttact aaacctgttc
     6901 accaacaata atcgtttatt cagtcaggaa ttcaatcgag atatggaaac gtttcagcct
     6961 ctatcctatg cagaagcaga taaattagcc gaatcgatct tggctcaaat gaccttagat
     7021 gagaaaatcg ctttgattgg cggcgataaa tacttcttta ttcgagcgct accgcgtttg
     7081 aatctttcgg aaatttatat gtcggatgcg actcaaggca tccatatccg tcagagcttt
     7141 aacgaaattg acctctcgaa ctaccaaccg gaaaaatcaa cagcctttcc ttgtccgatt
     7201 tcattagccg ccacatggaa tcgtgaattg gcttatcaat acgctcgagc gatcggtgaa
     7261 gaatgtaaag ccgccaatat tggcatttta cttggaccag ggatgaatat ttatcgtcaa
     7321 tcccaatgcg gtcgcaattt cgagtatttt ggcgaagatc cctacttagc ggcgcggatg
     7381 atagagaatt atgttcaggg agttcagagt actggaaccg tagccaccct aaaacatttc
     7441 ttagccaata ataccgaata ctatcggaag aaaagcaact cggtagttga tgaacnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttagc caataatacc gaatactatc
     7621 ggaagaaaag caactcggta gttgatgaac gaactttgca tgaaatttat gtgcccgctt
     7681 tcaaagccgg aatcgaagcc ggcgcaaagg cggttatgac ttcttataat ttgttaaatg
     7741 gtgaatggtg tggagaaagc gcttatgtca tcaattatct cttgagaaat cagcttggat
     7801 ttcaatggct ggtaatgacc gattggtggt cagtttataa cggtgagaaa ctggctaaat
     7861 ccggtcagaa tctggagatg ccgttcacaa ttgcgctgga aaatgtacat cagcttcttg
     7921 aggataataa agtgcaagta agcgacatag atcgaatggc gcaaagtatc ttgcgcacct
     7981 gttttgctat gaaattatat ggtcggtcgc gaactgcggt ggaagacgat ttctatgaag
     8041 cccatgaaaa agttgccttg caaaccgccc gagaaggaat agtgttacta aaaaatgaaa
     8101 acaacatttt gccggtaaaa gaacaggtaa aaaatatctt agttaccggc gattatgtca
     8161 aaaaattagc cgttggtggt ggttcagcgt atgttgaggg atacaataac cgtcaaatga
     8221 tttctgaact gcagaaagtt tttggtgatc ggataaaata tgtcaaaaat ccaaccgtaa
     8281 agcagatcaa atccgcccaa ttggtgctgt gcaatatagg cactagcgat agcgaaggct
     8341 gggaccgccc gtttgaattg ccggaagatc aagagcagaa agttcttaaa tgcgtcaata
     8401 ataatccgaa cacggtcgtt attgtgactt cgggaagtgg gatacgtatg actggctgga
     8461 atgagaaagc cagagcaata atctatgcct ggtatcctgg gcagattggg aatcaagcct
     8521 tggccgaaat tttaatcggt aaggttaatc cttcgggaaa attgccaatt accattgaaa
     8581 aggagttcaa agattcaccg ggttacggtt atattccgga aggcgaatcg ctttatttcg
     8641 attggaatgg ggaaggcgag agagcgcatc cggtatatga tgtgcgatat aaagagggaa
     8701 tatttatcgg ctaccggtgg tatgaatctc agaaaatcga accgctttat ccctttggac
     8761 acggactgtc gtttacatcg ttttcttata gtgatttgca agtgacaccg gggaaatttt
     8821 ccgaggttga taccatttca gtcacctttt cggttaaaaa tgtgggtaag tgccagggcg
     8881 ccgaaattgt ccaactatat gttagtgatc tcgaatcatc cttacctcgc ccagcgaagg
     8941 aattgaaagg ttttcagaaa atagtattag agccgggtga aattaaaact gtaaccttga
     9001 aattaagcca aaaggatttt gccttctgga gtcccgcaat taaaaattgg acgactgaaa
     9061 agggaaaatt tgttatattg ataggttctt cgtcacaaga tattagactt tccaaagaga
     9121 tcgaactatt ataaattcta aaagtataaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn accttctcga tcggtagagc ggaagcgatc ggtagcacaa ttttgcattt
     9301 taccgattat aaagatcgtc gcaatcattt tgcattttat tcggtcaacg ccccgattga
     9361 tggttttgac tgtgacaaag aatcctttat gggattgcat aatgggccac acgagccggt
     9421 agccgtgttc gagggcaaaa ctcgcaattc ggtggctttt ggctggtcgc cggtagccgt
     9481 ccatcaaatt gaggttaagc tcaatcctgg cgaatcgaaa agtttcaatt ttatcctcgg
     9541 ttatgtagaa taccccaaat ctgaaaagtt cactgccgac ggcaaaatca atttgaccaa
     9601 agtgcgtcag atgcaatctc gcttcgaatc gccggaaagt gtgacaaagg ctatgaatga
     9661 tttacgcgaa ttctgggatg gtttattatc ggtttttcaa gtccaaacag gcgaggaaaa
     9721 gattgaccgg attgtcaata tctggaatca atatcaatgt atggtcacgt tcaatatgtc
     9781 gcgtagcgca tcttatttcg agtccggaat tggacgagga atgggatttc gtgattccag
     9841 tcaggacttg ctcggcttcg ttcatcagat tccggagcga gcgcgggaac gtttgctcga
     9901 tattgcggca actcagttac cgaacggcgg tgcttatcat caatatcagc cactaagcaa
     9961 gcgaggcaat tatgaagtgg gcgacaattt taatgacgac ccgctctggc taatctgtgg
    10021 agttgtggct tatttgagag aaaccggcga ttttccgata ttggacgaat cggtaccatt
    10081 cgataatgat ccgaataatc aagccagcct gttcgaacat ctcaagcgtt ctttttatca
    10141 tgtggtaaat aatcttggac ctcataaact tccgctaatc gggcgcgccg actggaacga
    10201 ttgccttaat ctgaatattt ttaacactga tccggatcag tctttccaaa ccggtctatt
    10261 aaaaaccgac ggtaaaactg ctgaatcgat tatgattgcc ggggcatttg ttctatatgg
    10321 tcaggagttc attgaacttt gtgaatttct gggaaaaatg gatgaagcca ctgaagcccg
    10381 ccagcatatt gatgaaatga aaaaaagcat tgaccggttc ggctgggatg gtgaatggtt
    10441 tttgcgcgct tatgatgctt ttggaaggaa agtcggcagt caggaaaatg aggaaggcaa
    10501 aatttttatc gaatcgcagg gattctgttg tatggcagag atcggcaagg aagacggccg
    10561 gatgcaaaaa gcactcgatt ccgtaaaaaa atacctcgat tgtgaatttg gaattgtttt
    10621 gcaaaatccg gcttacacga aatattacat cgaattaggt gagatttcga cttacattcc
    10681 tggttataag gaaaacgc
//
LOCUS       DAOSMP010000059        10666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_6200_length_10666_cov_6.165960, whole genome shotgun sequence.
ACCESSION   DAOSMP010000059 DAOSMP010000000
VERSION     DAOSMP010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 10666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10666
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6200_length_10666_cov_6.165960"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..713
                     /locus_tag="PKX09_08600"
     CDS             90..713
                     /locus_tag="PKX09_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17921.1"
                     /translation="MGLFDIFKTKESNISSDKPLLPECPSEIIEREKKDILNRFIKGE
                     EASNFPSNILLKKGERLIIDIPNIILAEEKTTKVGGGYLGFSIRIMKGVSVRPGVFTG
                     SKETKVTQVDKGSFTLTNKRIIFTGDKQTRNFNLSSINSLLPTDNGIGVNRTGKTKTE
                     YYLGTQNVSIQFTIEPEEGENFKPTKIEWSLDGYNIQEIIQTLIQED"
     gene            884..3511
                     /locus_tag="PKX09_08605"
     CDS             884..3511
                     /locus_tag="PKX09_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOU17922.1"
                     /translation="MDDIKNYLISNNWNINYEYQIPEKNGEYFNIDDIGLSQSSKKYL
                     SIYKLGIYKHQKLAITKFLSGRNICITTSTSSGKSLTFYVIAMELLSKKPDSKIMAIY
                     PLKALGTEQTERWESAFRSSGLDISVGRIDGGIPVKERINIIKNNQIIIFTPDIIHAW
                     MLSNISTKAIINFLSNVEAIIVDEAHVYSGVFGSNSAFLYRRLNNICASLNKIPKYIC
                     ASATIKNPEVHMKNLIGLDFTIIDEKSDSAPKKRSDILLIDPPNNIDVLSAFSALLKY
                     ISNNTNHHFIAFVDSRKQTEYIATIATRLSDKDDSSEENLDFDELEQLKIFPYRAGYE
                     EIDRINIQNKLSSRELNGVVSTAALELGIDIPYLTLGILFGMPNSATSFYQRIGRIGR
                     NQNGVILLVNNKSILTEAIFRNPKILFKIPLTESTLYLENPRIQYIHTLCFARQSGEN
                     DIINQYLGTDTLLNIKTEFPENFIKLCNSERIGEISAEYQMMKSQAGEDPNHVFPLRD
                     VDIQFKVEYVRGPQKTMLGSLSYGQLMREAYPGAVYYYQTQSFRVTMVNIQKRLVQTR
                     RERKYTTSPTFLPSLIFPNLSGGNIYNFIKIGDLIIIESNIQVRETVIGYKERRGRTE
                     LQFVYPLNPNDTNGIYFNLPRFTRNYFTTGVILNHPSFNINNVKVPLLSEIIFESFLM
                     IVPLDRQDVNFGCDKHRASRHGLNEGSRFISIFDQTYGSLRLTSRIIENEILRNVLDK
                     SIELASNEKRYDMNLETIEAINQLRNSLNSKIEQLSINFGEFGNDKDYEKILLPNSEG
                     LDIARDNEEFYVESIFFSRDYNSLAYYGKHLSQQTKKFESAKISVPIKNIIGVPGKCE
                     YGYYNYSTGEICKELKKNA"
     gene            3697..3897
                     /locus_tag="PKX09_08610"
     CDS             3697..3897
                     /locus_tag="PKX09_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17923.1"
                     /translation="MAKIQVTNATEKTMEIRALVSTENSSKTWELRCLVREKLIDFIQ
                     QNYPDKLPRLRLETDKNTDVKN"
     gene            3973..5178
                     /locus_tag="PKX09_08615"
     CDS             3973..5178
                     /locus_tag="PKX09_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17924.1"
                     /translation="MKLPIFRKTAQISCLIIMVSVICRAQFLDDFDQKKVKGWFCLTG
                     DGDISMDLVPMDGYVRLIVDATRDKHNVWWATIKRDVTPFLDLGKLKEPDYELRVEAK
                     VRLSTAPHRINFMINTQRTTNFHEHLMEFDIPDTTGWHVVSYTTKHLDVVPGDTLYVQ
                     LSATDFGWEKYFVDIDYYRADVVNVKQAGADKGEAIPYHPPIPDVNTFALHLPVAHDC
                     VLNLDFPDVNFNDWQEVTAEGNARVLTVNGNQWAILRWDFGKYKGGKVAQAGLLEITT
                     FSVAQGGNYSAVYGDDFGMEFGKIRVIEILGGDPNWNQENVTYHNFMQGKPYPAVFNT
                     QMIYDADLSRTPEGKNFITISKPVMQRLLDGTTKGLLIRLLGAIDVSIYAVEDKTGQG
                     AKLHFNLKK"
     gene            5206..5967
                     /locus_tag="PKX09_08620"
     CDS             5206..5967
                     /locus_tag="PKX09_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17925.1"
                     /translation="MNNNIFKKELNIRRLMSYWSSPFTKSFGKLLLQSVLIIMILSVN
                     IFSAGQNPSIILIGKLGAEYSLNEDISITHNNPFIPTVSAGFEIPSLDFLHVYCNYNY
                     ARKMGHPYIISTTIHLDSNLNVESIEEEKWVDKDGEYLIKKTQVIIGLKGKLKVSDDT
                     YINIGAGISPFFRGLYDSKQSSGARATLKEQGRIIAGYVFSLELEKYLKDNLLIRCSM
                     DYLKLREEPYGIMITRSSPSFYFQVGYKLKIKVPE"
     gene            6171..10295
                     /locus_tag="PKX09_08625"
     CDS             6171..10295
                     /locus_tag="PKX09_08625"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOU17926.1"
                     /translation="MKRFKVLKMFIFLKFFLFLFWGVPNLVYSQYLNQIWQNVEFGSY
                     FGGSKIWCVDLNNDGSNEIICSKKAGFSEGADYFSVLEYIGNDYKISWTSKWYGTDTI
                     QAINIYDINQDGELEIFILLSSGNIDVYKSQTMELDTTYQTTINNARNLIISDVDNDG
                     QIEFILASDDDDDFKINIINSLSLAGEWDTTITLADDLDIKVGNVDNDVGIEIILSSG
                     IIIDGADHHIKWVYTEGFGEKIELADIGGNSVPEIIGISASNYITAFDAELQLLLWQF
                     NTFREYNSLYITDLEDDGEFEILVGNSRGGGISCYSSATKQLLWDINNPQYGVTNICT
                     GDPDRDGVKEIIWGVDQSSYGSGQLLIASSNLHQIEWQSADWNGPFSLDVFDFNYDGI
                     NEIAGASYKSNGGTGGGIALIYSGLNHIIKNATKQSVVGDAPINAIAAGNIDKFYQGE
                     LVAGVGNKIFVFDGDLAGISNQIEGLNIITSLAIADVDNDNTIEIVAGDIDGYLYIYN
                     GKKLTLEWKGMDTGDPIEKIVITNCDEDDAQEIIFLNGQSQINIYDGKTHELEWQMTD
                     FDNITSFDVADFDLDGNKEIILGHSNGMITTLTYPASEITNQVPVIDEAIYGLVVANI
                     DSSSANEILVGSSKVTVINAVDFSPIWESQPLGENVGFGNNLKVFDIDKDNYLDILVS
                     SSWGIFQFEATSMKPDHIPPQIIAYYPSPDISSIELDDEVKVIFSERIDESSLNEANV
                     FVTANDSATINTNITYIDSNNSIMIKPDDYLPMNSNIRVTLSGNIFDTSMNGLDGNKN
                     GISEGSPEDDFSWVFITGTNLDTLGPNISSVTVNSDSLWKGMDLTINVSVSDFFPYNF
                     SLVQSAECFIDTIGENGTGLPLIPLDGEFNAVDENCTITISTKDWADGEHHLFFHAQD
                     TKENWGKFHQITINIIVDMVANWPMLGQNPQHTGFNALDSIQLPLRVKWSKKLADKEM
                     NNLIVVNNFIYITSNGFFEDAILYALNIEDGSEEWVYFYGEIYLINPPAFAYGFVYAQ
                     TCNGSEGAFLNAFDARTGKLIWQAPMSAQWENYQAPTVADGKIFINGGYDGGIYCFDA
                     YTGEELWFVDLEPIINWTTAYYEGIVYTAGSSSSFKAINSTTGQILYEFGKYGIGYTP
                     AISNGVIYTVNPVSAIDLNTRKLLWSKPSKYTPAIGSGMVFSVASGKLNVYDEKTGQT
                     LWSFSGDSKLNHSPIVANGFILVSSNDNTYAINIKTQELVWHYEKGGRLTVANNRLYL
                     ATTSGEVVAFEKAPTSIEFEKSNSPLSFRLLQNYPNPFNPTTTISYELPKSTFVSLSI
                     YDIQGRLIKMLVNEWKSAGYHSVNWNAEKLGTGIYFYRLNTGEYSDVKKCIIIK"
     gene            complement(10417..>10666)
                     /locus_tag="PKX09_08630"
     CDS             complement(10417..>10666)
                     /locus_tag="PKX09_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17927.1"
                     /translation="SVGTRAYMAYDMGGKNNGLEGALASYCLGPRVGNELHYRKIRTL
                     EWLQLVPCVCIYPMIALPLEAYKGKTMTEIEIEEGLRK"
BASE COUNT         3634 a         1603 c         2126 g         3303 t
ORIGIN      
        1 aaaacaacta ggtagaacag aagatgcaat aagaaatgag gcatcgaaaa aaaatatttc
       61 attaaaacct aaagatatga ggaaataata tgggactatt tgatatattc aaaacaaagg
      121 aatcaaatat atcctcggat aaacctttat tgccagaatg tccgtctgaa ataattgaaa
      181 gagaaaaaaa agatatatta aatcgattta ttaaaggtga ggaagcaagt aactttcctt
      241 caaacatctt attaaaaaaa ggtgaacgat tgataataga tattccaaat attattcttg
      301 ctgaggaaaa aaccactaaa gttgggggtg gatatttagg attctctatc cgaattatga
      361 aaggtgtctc tgtcagacct ggtgtattta ccggttcaaa agagacaaaa gtaactcaag
      421 ttgataaagg atctttcaca ttaacaaata agcgtattat ttttacagga gacaaacaaa
      481 cgagaaactt taatctatct tcaataaact cattattacc gacggataat ggcattggag
      541 ttaatagaac aggaaaaaca aaaactgaat attatcttgg tactcaaaat gtaagcattc
      601 agtttacaat cgaaccagaa gaaggagaaa atttcaagcc tacaaaaata gaatggagtt
      661 tagatggtta taatattcag gaaattatcc agacgttaat tcaagaagat tgatatttaa
      721 gttctaacca tagtctggag gcaacgaggt gagctctggc ataattttga gatttcagac
      781 ttttcaaggg ggtattgaac tgtgaaagtg tatcgcttcg gttcacctcg tgcctcatca
      841 ttgtccgtta gacaatatac aactaacata gaggattctt acaatggacg atattaaaaa
      901 ttatttgata agtaataatt ggaatatcaa ctatgaatat caaataccag aaaagaatgg
      961 agaatatttt aatattgatg atataggatt aagtcaatca tcaaagaaat atttgagtat
     1021 atacaaattg ggaatataca agcaccaaaa actagctata acaaaatttt tatccggtag
     1081 gaatatatgc ataacaacct caacctcctc tggaaaatca ttgacctttt atgtaatagc
     1141 aatggaattg ttatctaaaa aacccgattc aaaaataatg gcgatatatc ctttaaaagc
     1201 attgggtact gaacaaaccg aacgatggga atctgctttt agatcttctg gattagatat
     1261 ttcagttgga agaattgatg gagggattcc cgtaaaagaa agaataaaca ttattaaaaa
     1321 taatcagata ataattttta ctccagatat aatacatgct tggatgctat caaatattag
     1381 tacaaaagca ataattaatt ttttatcaaa tgtagaggcc ataattgtag atgaggctca
     1441 tgtttattct ggcgtatttg gtagtaatag tgcattccta tatcgtaggt tgaataatat
     1501 ttgcgcttcg ttaaataaaa ttcctaaata tatatgtgca tcagcaacca ttaagaatcc
     1561 tgaagttcat atgaaaaatc tgattggact agatttcacg attattgatg aaaaatctga
     1621 ctcagcgcct aaaaagagaa gtgatatttt actaattgat ccacctaata atatagacgt
     1681 actttctgcc ttttcggcat tattgaagta catttcaaac aacactaatc atcattttat
     1741 tgcttttgtc gacagtagaa aacagactga atatattgcg actattgcaa caagattatc
     1801 tgataaagac gattcctcag aagaaaatct agattttgat gaattggaac agttaaaaat
     1861 atttccatac agggcaggtt acgaagagat tgatcgaatt aatatacaaa ataaactatc
     1921 tagcagagaa ctaaatggag ttgtatctac agctgctctt gagctgggta tagacatacc
     1981 atatttaaca ttaggtatat tgtttggaat gccaaattct gcaacgagtt tttatcaaag
     2041 aattggtaga atcggtagaa atcagaatgg agtaatttta ttggttaata ataaatcaat
     2101 tctaaccgaa gcaatattca gaaatcccaa aatactattc aaaatacctc tgacagaaag
     2161 tactttatat cttgagaatc cccgaataca atacatccac acgttatgtt ttgcgaggca
     2221 atccggagaa aatgatatta ttaaccaata tcttggtaca gatactttac ttaatataaa
     2281 aactgaattt cccgaaaatt ttataaaatt atgtaattca gagagaattg gtgaaatttc
     2341 cgcagagtat caaatgatga aatctcaagc aggcgaagat cccaatcacg tttttccatt
     2401 acgagatgtt gatatacagt ttaaagttga atatgtacgt ggccctcaga aaacaatgtt
     2461 aggatcacta tcatacggtc aattaatgag agaagcatat cctggtgctg tatattacta
     2521 tcagacacaa agttttagag taacaatggt taatattcag aagagattag ttcagactag
     2581 acgcgaaaga aaatatacta cttctccaac atttctacca tcattgatat ttccgaattt
     2641 atcagggggt aatatttata attttataaa gataggcgat ctaattatta ttgagtcaaa
     2701 tattcaggta agagaaacag taataggata taaagaaaga cgaggaagga ctgaacttca
     2761 gtttgtttat cctttaaatc caaatgatac taatggtata tatttcaatc taccaagatt
     2821 tactcggaat tattttacaa caggagttat tcttaatcat ccttctttta atataaataa
     2881 cgtaaaagta ccattattat ctgaaattat ttttgaatca tttttaatga tcgtcccttt
     2941 agatcgacaa gatgtaaatt ttggttgtga taagcataga gctagccgac atggattaaa
     3001 tgagggtagt aggtttattt cgattttcga tcaaacctat ggaagtttga gattaacaag
     3061 tagaatcatt gaaaatgaaa ttcttcgtaa tgttttagat aaatctattg aattagcctc
     3121 aaatgaaaaa agatatgata tgaatctcga gacaatagaa gcaattaatc aactaaggaa
     3181 tagtttaaat agtaaaattg aacaattatc aattaatttc ggtgaatttg gtaatgataa
     3241 agattatgaa aagatactat tacctaattc tgagggttta gatattgctc gagataatga
     3301 ggaattttat gttgaaagca tattttttag tagagattat aattcattag catattatgg
     3361 gaaacattta tcacaacaaa caaagaaatt tgaatcagct aaaatatctg tcccaattaa
     3421 aaatattatc ggtgtccccg gaaaatgcga atatggatat tataattaca gtactgggga
     3481 aatctgtaaa gaattgaaaa agaatgcata accactggct ggaggcaacg gcttaagctg
     3541 ttcgatgaaa gtgaagtttt acgagttttc tgaagatttg agaataaaca gaagcaaatc
     3601 acttagtcac ccagcgtggc tgagcattaa tcataattga ttcggaagaa gaatagccgt
     3661 acagttgtaa atgcttcttc actttgggac aaacgtgtgg caaaaatcca agtcactaat
     3721 gcgacggaaa agacgatgga aatacgggca ttggtaagta cggagaattc atccaaaact
     3781 tgggaattgc ggtgcctagt gcgagaaaag ttgatagatt ttatccagca aaactatcca
     3841 gataaattgc ctcgtctaag attagagacc gacaaaaata ccgatgtaaa aaattaaaca
     3901 ttcaggttat taaaagtgac tatgtaaatt atgttttagc taatgacttc ataatagaag
     3961 taaaggagaa taatgaaact accaattttt cgtaagacag cccaaatatc ctgcctgata
     4021 attatggttt ccgtaatttg tcgcgcccag tttttggacg atttcgatca gaaaaaagtc
     4081 aagggctggt tttgtttgac cggcgatggc gatattagta tggatttagt gccaatggat
     4141 ggttacgtgc gtcttatagt tgatgctacc cgcgacaaac ataatgtatg gtgggctacc
     4201 atcaaacgtg acgttacgcc ttttctcgat ttaggaaaat tgaaggagcc ggactacgaa
     4261 ctgcgggtcg aagcgaaagt gcggctaagt acggcgccgc atcgcattaa ttttatgata
     4321 aatactcagc gcacgacgaa tttccatgaa catctaatgg aattcgatat tcccgatact
     4381 accggctggc acgtagttag ttacacgacc aaacatctag acgttgtgcc cggtgatact
     4441 ctttatgtcc agctttcggc tactgatttc ggttgggaaa aatatttcgt tgatattgat
     4501 tattaccgcg ccgatgtagt taatgtcaag caagccggtg ccgataaagg cgaagcgata
     4561 ccttatcatc cgccgattcc ggatgtcaat acttttgcgc ttcatctacc ggtggctcac
     4621 gattgcgtgc tgaatcttga ttttcccgac gtcaatttta atgactggca ggaagtaacg
     4681 gcggaaggta atgcgcgggt attaaccgtg aacggcaatc aatgggcgat tttaaggtgg
     4741 gatttcggaa aatataaagg ggggaaagtc gctcaagccg gtttgctgga aattactact
     4801 ttctcggtag cgcagggcgg caattatagc gcagtttacg gtgatgattt cgggatggaa
     4861 tttggcaaaa ttcgtgtgat cgagatttta ggcggcgacc cgaactggaa tcaggagaat
     4921 gtcacttacc ataattttat gcaggggaaa ccgtatcccg cagttttcaa tactcaaatg
     4981 atttatgatg cggatttatc ccgaactccc gaaggtaaaa atttcattac gatttcaaaa
     5041 ccggtaatgc aacgtttact cgacggtact actaaaggtt tgctcattcg actgttggga
     5101 gccatagatg tgtcaatcta tgcggtggaa gataaaactg gacaaggcgc caagttgcat
     5161 tttaatctta aaaaatagtt gaatccaagg gttgcggggg aataaatgaa taataatatt
     5221 ttcaaaaaag aattaaatat cagaaggttg atgagttatt ggagttcacc ttttactaaa
     5281 tcgtttggta agctattact ccaatcggta ttgattataa tgatattatc agtgaatatt
     5341 tttagtgctg gacaaaaccc ttcgattata ttgatcggaa aattaggcgc cgaatattcc
     5401 ctaaatgaag atatttcaat aactcataat aatccgttta tccctacagt ttcggccggt
     5461 tttgaaatac cgtcgcttga ttttctgcac gtttattgta attataacta cgcccggaaa
     5521 atgggacatc cctatattat ttctacgacc atccatttag attctaactt aaatgtcgaa
     5581 tctattgagg aagaaaaatg ggttgataaa gatggagaat atttaataaa gaaaactcag
     5641 gtgattattg gattgaaggg taagctgaaa gtttccgatg acacttatat taatatcgga
     5701 gcaggtatct ctcctttttt tagagggtta tatgattcga aacagagtag cggcgcaaga
     5761 gctacactta aagagcaagg gcgaataatt gccggttatg tgtttagtct ggaattggaa
     5821 aaatacctta aggataattt attaatacgg tgttcaatgg attacttgaa attaagggaa
     5881 gaaccatacg ggataatgat aaccagaagt agccctagct tttattttca agtagggtac
     5941 aaattgaaaa taaaggtgcc cgaataatgc taaaaatgaa cgattataat tgtttgatag
     6001 atacaaactt taggattcta aataaatatt atgatgaaat aaataatcat ctgtttttac
     6061 tggcattatt ttttatctat tgattaggtt tggaaatgtt taattaaata ataaattagt
     6121 tttagaaata tttctttaaa aggtgagcgt atgaaaggag gcatatagaa gtgaaaagat
     6181 ttaaagtctt aaagatgttt atatttttaa agttcttttt attcctcttt tggggagttc
     6241 ctaatctggt atattctcaa tatttaaacc agatttggca aaatgtagaa tttgggagtt
     6301 atttcggagg tagtaaaatc tggtgcgttg acttaaataa tgacggttct aatgaaatta
     6361 tttgtagtaa aaaagccggt tttagtgagg gcgctgatta tttctcggtt ttggagtata
     6421 ttggtaatga ttataaaata tcgtggacga gtaaatggta tggcaccgac acaatccaag
     6481 cgattaatat ttatgatatt aatcaggatg gagagttaga aatatttata ttactttctt
     6541 caggaaatat tgacgtttat aaaagtcaaa cgatggaact ggatacaacc taccaaacga
     6601 caataaataa tgctcgtaat ctgatcattt cagatgttga taatgatggc caaatcgagt
     6661 tcatcttggc aagtgatgat gatgatgatt tcaagataaa tattatcaat agtctatcac
     6721 ttgctggaga atgggataca actattacgt tggctgatga tttagatatt aaggtcggta
     6781 atgttgataa cgacgtcgga atcgaaatta tcctatcgtc gggtattatt atagatggcg
     6841 ctgatcatca tattaaatgg gtttatacgg aaggatttgg agaaaagatc gaattagctg
     6901 atattggtgg gaattcagta ccagaaatca ttgggataag tgcctcaaat tatataactg
     6961 cgtttgatgc tgaattacag ttacttctct ggcaattcaa tacttttaga gagtataact
     7021 cattatacat tacagatttg gaagacgacg gtgaatttga aatattggta ggtaatagcc
     7081 gaggcggagg aattagttgc tatagttctg caaccaagca actattgtgg gacattaata
     7141 atcctcaata tggggtaacg aatatatgta caggtgatcc cgatagagat ggagtaaaag
     7201 agattatttg gggagtagat caatcttctt atggttctgg gcaattgttg atagcaagta
     7261 gtaacctgca tcaaattgaa tggcaaagtg ctgattggaa tggccctttt agtcttgatg
     7321 tttttgattt taactatgat gggattaatg agatagccgg agcaagttat aagagtaatg
     7381 gcggtacagg aggagggatt gctcttattt atagtgggtt aaatcatatc attaaaaatg
     7441 ctacgaagca gagtgtcgtt ggggacgctc cgataaatgc tattgccgcc ggtaatattg
     7501 acaaatttta tcaaggtgaa ttggtcgccg gggttggtaa caaaatattc gtttttgatg
     7561 gcgatctagc tggaatatct aaccaaattg agggattaaa tatcattacc agccttgcca
     7621 tcgccgatgt tgataatgat aatacgattg aaatagtagc aggcgatata gacggttacc
     7681 tttatatata taatggtaaa aaattgacgt tagaatggaa aggtatggat acaggtgacc
     7741 caattgagaa aattgttatt acaaactgcg atgaggatga tgctcaagaa ataatatttt
     7801 taaatggtca gagtcaaatc aatatctatg atggcaaaac acacgaatta gaatggcaga
     7861 tgactgattt cgataatatc acttctttcg atgttgctga ctttgatctt gatggaaata
     7921 aagaaataat tcttgggcat tccaatggta tgattacaac cttaacttac ccggcttccg
     7981 aaattacgaa tcaagtaccg gtaattgatg aagctattta tggactggtc gtcgctaata
     8041 ttgattcatc ttcagctaat gaaattttag tcggcagttc taaggtaacc gttatcaatg
     8101 cggttgattt ttcgccaata tgggaaagtc aaccgctagg agaaaatgta ggatttggaa
     8161 acaacctaaa agtatttgat attgataaag ataactactt agatatttta gtaagtagtt
     8221 catggggaat ttttcaattt gaagctacca gcatgaaacc agatcatatt ccgcctcaaa
     8281 ttattgccta ttatcccagt cctgatatat cgagtataga acttgatgat gaagtaaaag
     8341 ttatattctc ggaaagaatt gatgaatcaa gtttaaacga agccaatgtt tttgttactg
     8401 ctaatgattc cgctacgatc aatacgaata tcacctatat tgatagtaat aattcaatta
     8461 tgattaagcc agatgattat ctaccgatga atagtaatat tcgggtcact ttgtcaggaa
     8521 atatttttga tacctcaatg aatggtctgg atggcaataa gaatggaatt agcgaagggt
     8581 ctcccgaaga tgatttttca tgggttttca taacgggaac taatttagat actcttggtc
     8641 caaacatatc gtccgtgacc gtaaattcag attcattatg gaaaggtatg gatttaacaa
     8701 taaatgtatc agtttctgat tttttccctt acaatttctc acttgtccaa agcgcggaat
     8761 gctttataga caccatcggt gaaaatggaa ccggattacc attaatacct cttgatggtg
     8821 aatttaatgc agtagatgaa aattgtacta ttaccataag taccaaagac tgggcagatg
     8881 gtgaacatca tctatttttt catgctcagg atactaaaga aaattggggt aaatttcacc
     8941 aaattactat caatattata gtggatatgg tagccaattg gccaatgttg ggacaaaatc
     9001 ctcagcatac cggattcaac gcacttgact ccattcagct accgttaaga gttaagtggt
     9061 ctaaaaagtt agctgataag gaaatgaata atttgatcgt cgtcaataat tttatttaca
     9121 taacgagcaa cggctttttt gaagatgcaa ttctttatgc gttaaatatt gaggatggtt
     9181 ctgaagaatg ggtctatttt tatggagaaa tttatttgat taatccgcct gcttttgcct
     9241 atggttttgt atatgctcag acctgtaatg gttcagaggg tgcgtttctc aacgctttcg
     9301 atgcaaggac gggtaaatta atttggcagg cgcctatgag tgcacaatgg gaaaattatc
     9361 aggcaccgac cgttgcagac ggtaaaattt ttattaacgg tggctacgat ggtggaatat
     9421 attgttttga tgcctatacg ggtgaggaat tatggtttgt ggatctagaa ccgataatca
     9481 attggactac agcgtattac gaggggatag tctataccgc tgggtcaagt tcttccttta
     9541 aagccatcaa ttctacaaca ggtcaaattt tatacgaatt tggtaaatat ggtattggtt
     9601 atacaccggc gatcagtaat ggagttatct atactgtaaa tcctgtaagt gctatagact
     9661 taaatacgcg aaaattgtta tggtctaaac ccagtaaata taccccggct attggttctg
     9721 gaatggtttt ttcggtagca tccggaaaat taaatgtata tgatgaaaaa actgggcaaa
     9781 cactctggtc attttcggga gatagcaaat taaatcattc tcctatagtt gctaatgggt
     9841 ttatcctggt gagttctaac gataatactt atgcaatcaa tatcaaaacg caggaattag
     9901 tctggcatta tgaaaaaggt gggcgtttaa ccgtagcaaa taatcgtttg tatctcgcaa
     9961 caacatctgg agaagtcgtt gcatttgaaa aagcacctac ttccattgaa ttcgaaaaaa
    10021 gtaattcgcc actgtctttt agactattac aaaattatcc gaatccgttt aatccgacga
    10081 cgactatttc ttatgagtta ccgaaatcaa cttttgttag tttgtctatt tatgacattc
    10141 agggcagatt aataaaaatg ctcgtaaatg aatggaaaag tgccgggtat cattctgtca
    10201 actggaatgc tgaaaaatta ggaactggta tatattttta ccggcttaat actggtgaat
    10261 atagtgacgt taaaaagtgt ataattatca aatagtacca tataaacata ttcttatctt
    10321 gacagtaaaa agcgtgatat aaatttaagg attgttcttc gaaaaatgag aattattggg
    10381 caaaagattg attactaaat aatttttgaa catatgctat tttcgcaatc cttcttcgat
    10441 ttcgatttcg gtcatcgttt tgcctttgta ggcttccagt ggaagggcaa tcatcgggta
    10501 aatgcagaca cagggaacca gttgaagcca ctccaatgtc cgaatcttac gatagtgtaa
    10561 ttcattaccg actcgaggcc cgaggcaata actcgcgagg gcaccttcga gaccgttgtt
    10621 tttaccgccc atatcataag ccatataggc tcgtgttcca actgaa
//
LOCUS       DAOSMP010000060        10437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_6382_length_10437_cov_14.573204, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000060 DAOSMP010000000
VERSION     DAOSMP010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10437
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6382_length_10437_cov_14.573204"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            810..3977
                     /locus_tag="PKX09_08635"
     CDS             810..3977
                     /locus_tag="PKX09_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOU17928.1"
                     /translation="MKQILEKYLANLSDTFNRGDAREESYYKHLDDMIKQCAEIQDIK
                     NIEVTILPKKTDAGNPDFRVWDGQNHITGYIEAKDPSITDLDYIEDSEQLKRYISTFP
                     NVILTNFYEFRLYRDGERIRRVMIGRPVIAQKLKTKPPLENIEQFIELFDLFFSFSLP
                     KVQNARSLAVELAKRTRFLRDEVIAVEMAENGSKGQKQIAGFYEAFKTYLIPTLTAEQ
                     FADLYAQTITYGLFAARTRVTSERDEFNRKIAFDYIPHTIGILRDIFHFISLGDPPKS
                     LQIIVDDIVEILNVADVNKILHEYQRAGKGRDPIIHFYETFLATYDPKIRERRGVYYT
                     PEPVVGYIVRSIHSILKSHFNLADGLASEEVKLLDPAGGTLTFPAEAIRLAADEFKSK
                     YGEGGLHLWIKKHILANFHAFELMMAPYAIGHLKIGFILDELGYKMAEDERFKLYLTN
                     TLEMEEIKEITIPLLSSLSEESHFAEKVKKEQPILVILGNPPYSGISANINKWTERLL
                     KEDLDGAQSYYKVDGQPLGERNPKWLQDDYVKFLRFAQWKIQQSGYGIVGMITNHSYL
                     DNPTFRGMRQSLMKTFDEIYILDLHGNSLKKETTPAGGKDENVFDIRQGVAIALFVKN
                     KKPKESRVFHADLYGLREEKYQWLQKNDFRKNNYEKIKPTSPYYFFKKIDTEKVKQYL
                     KWEQITDIFPVNSVGIVTARDHFVISFDKRDLKNRILQLQNLAHPDEIILQAFDLKEK
                     FGWKIKDSRQEIANISDLDIYIKEILYRPFDKRYLFYHQSLIERMRFEVMQHMLEENI
                     GLVTIRRSRNPEKWNFAFVANKLIAGATAITSLDINYLFPLYLYPEQSKKDNPGLNLT
                     TMLIFEPGEKYGRKPNIAPIIFSGLAEYYPKRPSPEEIFYYVYAILYSNIYRETYVEF
                     LKIDFPRVPFTADYEVFKNLGKSGKQLADLHLLKSPALDPPIARYQGSSPNDRIEKVI
                     YQEDEQRIYINNDKYFEGIAPEVWNYYIGGYQVLHKYLKDRKGRTMDDAPHYCRIVTA
                     LSKTIEIQKQIDAIYPDVEKEIIKF"
     gene            4053..5993
                     /locus_tag="PKX09_08640"
     CDS             4053..5993
                     /locus_tag="PKX09_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008097421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HOU17929.1"
                     /translation="MNYKSVMPIWIAFLLLVSVFIADLNSANAPDIHTWEMQEIVLQA
                     EGEYENFYTDVTCWIELTGPEFAKRIYGFWDGANIFKVRFVATKPGKWYLRSGSNQPN
                     DNGLNSLSGEFMAVEWTETEKLQNPNRRGFVRSTSNGHALQYADGTPFFMIGDTWLAG
                     STWRLPFRNADSPENYEPAPGIGFEDAVKYRKIQGFNSVSIISCFPNWDADCKPSTYA
                     DENGVFVRNAWEKFGYDVAEATGIDASGGLSYWGTLTAKEMRDEYGNLPFAMSQIHKG
                     VSDFNRINPQYFQSLDKKMEFLSQEGFVPLLETVRRDVGPSWYAYFDFNETFARFVQY
                     LIARYGAYNIIFSGIHLDWIPQEFSLTAEQFNEALTYHLEKYGPMPFDQPVTTLISSS
                     TYSVFGHHQDCPWLTMHSVGNKPRDHRVADSLEIIFRLEPPYPAINFEPYYTGWDHEI
                     NMPNGERPPANSDRDNYFARAQMYGSVLSGGLSGHVHGTSAYDITTTGEPVGKRHHIW
                     EALKFESANYMKHLSSFILSEGNKYQDLRLAREDIHPNKAPNSPPTGLDGWAYMMRTP
                     DKNLAMLYFENQSVLPTLSGFKPNTMYHFKWFDPRNGEWKEAQTIQADKKGNLKVPLF
                     PDGNNPSIKDWAAKICKEEKNK"
     gene            6024..7601
                     /locus_tag="PKX09_08645"
     CDS             6024..7601
                     /locus_tag="PKX09_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HOU17930.1"
                     /translation="MKKLVIGILGIFLMQNLYAQNVENKIVIDVEAGQHVISRHIYGH
                     FAEHLGRCIYGGIWVGEDSPIPNTRGIRNDVVAALKDIAAPNVRWPGGCFADEYHWMD
                     GIGPRDQRPKMINTHWGGVTEDNSFGTHEFMDFCEQVGCEPVITGNIGSGTVREMAQW
                     VEYLTSDNVSPMTELRKKNGREKPWRVRFWGLGNENWGCGGNMTADFYADEMRRFSTY
                     CKNYSGNELYRVACGPSDENYEWMETLMKEPINRNCFQGISLHYYTVCHDWTNKGSAT
                     NFNEKDWFLTMSKTLRMDEFVTKHSAIMDKYDPEKKIGLIVDEWGNWHDVEPGTNPAF
                     LYQQNTLRDALVAATNLNIFNNHCERVKMANIAQMVNVLQAIILTNEDQIVLTPTYYV
                     YKMYKVHQDATLLPIELTCQNYELADGSIPAVNASASVDKQGIIHITLCNLDPNKNMN
                     LTCELKSKNKFSVSSGSIITAGKMNSFNDFGKPEEVNLQKFTDCKIAQNTLSVNLPAK
                     SVVMVELVSKSADKPKK"
     gene            complement(7688..8170)
                     /locus_tag="PKX09_08650"
     CDS             complement(7688..8170)
                     /locus_tag="PKX09_08650"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOU17931.1"
                     /translation="MAKSPFIITILFWSSICFSQEKTADIQIYTSEFERIEIVRLRSG
                     MDLLEGLKEAVSSRKIKNGVILGGIGAVTDYHYHVVSSKNLPPDEEYPKASVPMDLIS
                     VQGYILNGRVHAHIALSDANSVVGGHLESGTKALTFFIITIGVLPDSLKIDNLDNYKY
                     "
     gene            complement(8220..8864)
                     /locus_tag="PKX09_08655"
     CDS             complement(8220..8864)
                     /locus_tag="PKX09_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17932.1"
                     /translation="MLKIYRRRQFHLTFLVVFSLGLIVLLLNDCTTRPQTSVAQYQFT
                     FEGEVYRIRSIYAVENNERYNELIGKNFLAVDFDQDRIIDKISVGTTTLAEAQKVYDY
                     GLEMLTKENRLKEITPENHKYIEDLEDFHLELKSFYPVNAKPFNQFKIISKKQLVNPE
                     AAVAIDHDADGILDEISKGTLSIQQAQIQYDNLINIGLQKNRLVKVDNTILVKR"
     gene            9296..9478
                     /locus_tag="PKX09_08660"
                     /pseudo
     CDS             9296..9478
                     /locus_tag="PKX09_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855261.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
     gene            9583..10404
                     /locus_tag="PKX09_08665"
     CDS             9583..10404
                     /locus_tag="PKX09_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOU17933.1"
                     /translation="METNKRIFIILFLMILITGCYTYRFSEKQFFNNKQGIELDSCYV
                     MEYVFYRNTENITLEGIIYFNKDATINVVFFPGISGNLNLYKYLANTLAQKLNANLFI
                     PNYRGYGYSEGIPTMKNLISDTKDQIEYFLNYPKVNRRLPLFIMGHSFGTNIALESAK
                     MENIKGVILIAPFSTNEEVIEHLKKKIVPFYLCPFIRIEPQERLKNIDNYELLRVIDK
                     PVLILHGTDDDELPIWMSEHLVNNLNNNKIELIKISGAGHFDIFINQKHSNLNNS"
BASE COUNT         3294 a         1995 c         2138 g         3010 t
ORIGIN      
        1 agagcagcgg actcttaatc cgttggttca aggttcgagt ccttgttggg gtacacctta
       61 aaatacaaat gtttaaataa tttagaacct cctatctaat ttctattagc cttctaaaaa
      121 tcggtataat ttaacttttc aagccggata ccggattctt ccagttgaaa tagtggttag
      181 acgacaagca ggtagatatt ccggagggaa aaaagcgtta aaaagttgaa aaatagtctc
      241 ctttatccca tatagggaat agcttttcga tttctattgg tctataatac cttaaccgct
      301 gagcggtatc cataatcact actgcatgac tacgaatcgc taccgaacac aacactctac
      361 aatatcttga ccacaaagcg gcgggtgtca atctaacgac aatagaatcc aaaatagacc
      421 ggaggtttta aagtattgta gaaattattt tattgaaaaa aattatttac cctgtagagg
      481 ttaaactttc aataaaaatg ggaaaaaacc tgtaattaaa acacattgac tttaactgaa
      541 gatttaccta atattgtttt gctacgaaat atggcagtaa attgttttat attataagag
      601 gaaaaagcaa cttaaatagc aatatcaaga cctgtttatt ctcataaatt ttctaaaatt
      661 acaaacgatt attgaatgga gaaaaatcgt aaaaatctca aaacctaaca ttgaaaaatt
      721 atagcactaa tttggaaata tctggaaaat atttttactt gcaatatcgc ctaaattgtc
      781 atttaacata taaaaaacat tctggttaag tgaaacagat actagaaaaa tatttagcga
      841 atctatcaga tactttcaat cggggtgatg cccgcgagga aagttattac aaacatctcg
      901 atgacatgat taaacagtgc gcggaaattc aagacattaa aaatattgaa gtaacgattc
      961 ttcccaaaaa gactgatgcc ggaaatcctg acttccgagt ttgggacggt caaaaccaca
     1021 tcaccggtta catcgaagcc aaagaccctt cgattaccga tctggactat atcgaagatt
     1081 ccgaacaatt aaaacgctat atatccacct ttcccaatgt tatcttaact aatttttatg
     1141 aattccgact ttatcgggac ggagagcgaa tccggcgagt tatgattgga cgaccggtaa
     1201 ttgcccagaa gctaaaaacc aaaccgccgc tggaaaatat cgagcaattc attgaactct
     1261 ttgatttatt cttttccttt tctctgccca aagttcaaaa tgcgcgttcg ctggcagtcg
     1321 agttagccaa acgcacccga ttcttgcgcg atgaggtaat tgcagtcgaa atggcggaaa
     1381 atgggagcaa aggtcagaag caaatagcgg gcttttatga agcctttaaa acttacctga
     1441 taccgacttt aaccgcagaa caattcgccg atctttatgc tcaaactatc acatacgggt
     1501 tgttcgccgc tcggacaaga gttacatctg agcgggatga atttaatcgt aaaattgcct
     1561 tcgattatat tccccatact atcggaattt tgcgggatat attccacttt atctcgctcg
     1621 gagatcctcc gaaatcactc caaatcatcg tagacgatat tgtcgaaatt ctaaatgttg
     1681 ccgacgtcaa taagattttg catgaatacc agcgcgccgg aaaaggccgg gatccaatta
     1741 tccattttta tgaaactttt ctggctacct acgatcctaa aatccgtgaa cggcgcggag
     1801 tatattatac cccggaaccg gtggtcggat atatcgttcg ttcgattcat tccattttaa
     1861 aatcgcattt taatctggcg gacggtctcg ccagtgaaga agtaaaactg ctcgacccag
     1921 ccggtggtac gctcaccttt cccgccgaag ccatccggct ggcggctgat gaattcaaaa
     1981 gcaaatacgg cgaaggcggt ttacatttat ggataaagaa acatatcctc gccaattttc
     2041 acgcttttga gctgatgatg gcgccctatg ccatcggtca cttgaaaatt ggttttattc
     2101 ttgacgaact cggttacaaa atggctgagg atgaacgctt caaactttat ctgaccaaca
     2161 cgctggaaat ggaagaaata aaggaaatta caattccctt actttcttcg ctgagtgaag
     2221 aaagtcattt tgcggaaaaa gtaaagaaag aacagccgat cctagtgatt cttggtaatc
     2281 caccttatag cggcatttcc gccaatatca ataaatggac ggaacgactg ctaaaagaag
     2341 accttgacgg cgcccagagt tattacaaag tagatggtca gccgctggga gaaaggaatc
     2401 ctaaatggtt gcaggacgat tatgtcaaat ttctacgctt cgcgcaatgg aaaatccagc
     2461 aatccggtta cggcatcgtg gggatgatta ccaatcacag ttatctggac aatcctactt
     2521 ttcgcggaat gcgtcaaagt ctaatgaaaa ccttcgatga aatttacatc cttgatttgc
     2581 acggcaatag cctgaaaaaa gaaacaactc ccgccggtgg caaagacgaa aatgtgtttg
     2641 atattcgtca gggcgtggca attgcgttat ttgtgaaaaa taaaaagccg aaggaatcca
     2701 gggtatttca cgcagattta tatggactgc gtgaagaaaa gtatcaatgg ctgcaaaaaa
     2761 acgattttcg aaagaacaat tatgagaaaa ttaaaccgac aagcccttat tatttcttta
     2821 aaaagataga tacagaaaaa gtaaagcagt atttgaaatg ggaacaaatt actgatattt
     2881 ttccggtgaa tagtgtaggt attgttacgg cgcgcgatca ttttgtcatt agttttgata
     2941 aaagagattt aaaaaacaga atattacaat tacaaaatct tgctcatccg gacgaaatca
     3001 ttttacaggc tttcgatttg aaagagaaat tcggttggaa gatcaaagat tcccggcaag
     3061 aaatagcaaa catcagtgat cttgacattt atatcaaaga aatattgtat cgtccatttg
     3121 ataaacgcta cctattttac catcaatctc tgatagaacg tatgcgcttt gaagttatgc
     3181 aacacatgct ggaagagaat attgggttgg taactattcg aaggtcaaga aatccggaaa
     3241 aatggaattt tgcttttgtt gccaataaat tgattgctgg tgcgactgcc attacatctt
     3301 tggacataaa ttacctattt cctttatatc tttatccgga gcaatcgaaa aaagacaatc
     3361 ccggtctgaa tctgactacg atgctgattt ttgagccggg cgaaaaatac ggccgaaaac
     3421 ctaatattgc gcccataatt ttttcaggat tggcagagta ttatcccaaa agaccgtcgc
     3481 cggaagagat tttctattac gtttacgcaa tcttatacag caatatttac cgcgaaacct
     3541 atgtcgagtt cttgaaaatt gacttcccgc gcgtgccttt taccgctgat tatgaagtgt
     3601 ttaaaaatct aggcaaatcg ggaaaacaac tggctgacct gcatctgctt aaaagcccgg
     3661 cgctcgatcc gccgattgcc cgataccagg gcagtagccc aaacgaccga atcgagaagg
     3721 ttatttacca ggaagatgaa cagcgcattt acattaataa tgacaaatat tttgaaggca
     3781 ttgcaccgga agtctggaat tattacatcg gcggttatca ggtattacat aaatatttaa
     3841 aagaccgtaa gggaagaact atggacgatg cgccccatta ctgtcgcata gtaaccgcct
     3901 tgagtaaaac cattgaaatc cagaagcaaa tagacgcaat ctatcccgac gtagaaaagg
     3961 aaatcataaa gttttaaaca atgaatttta ttgaaattgg tcatgcgatt attcaaattt
     4021 ttcatttcgt tatgaaagga tactaagttt agatgaatta caaaagtgtg atgccgattt
     4081 ggattgcctt tctgctttta gtaagtgttt tcattgccga tctgaattca gctaatgcgc
     4141 cggatattca cacatgggaa atgcaagaaa ttgtcttgca ggctgaagga gaatatgaaa
     4201 atttctatac cgatgtaact tgctggatcg aactaaccgg gccggaattt gccaagcgga
     4261 tttacggttt ctgggatggc gctaatattt tcaaagtgcg tttcgtcgct actaagccgg
     4321 gaaaatggta cttgcgcagt ggttcaaacc agccgaatga caatggacta aacagcctca
     4381 gcggcgagtt tatggcggtc gaatggacgg aaaccgaaaa attgcagaat cccaaccgac
     4441 gcggttttgt acgctccacc tctaatggtc acgctctcca gtatgctgac ggaacgccct
     4501 tttttatgat aggtgatacc tggctggcgg gatcaacctg gcgacttcct ttccgcaacg
     4561 ccgattcacc ggaaaattac gagcctgcgc cgggaatcgg ttttgaagat gccgtgaaat
     4621 atcgcaagat acagggcttc aattccgtaa gtatcatttc gtgtttccca aactgggacg
     4681 ctgattgtaa gccgagtact tatgccgatg aaaatggagt tttcgtcaga aatgcctggg
     4741 aaaagttcgg ttatgatgtt gctgaagcaa ccggaattga cgcctccggc ggtttgagtt
     4801 attggggcac gctgactgcc aaagagatgc gcgatgaata tggtaatctg ccttttgcaa
     4861 tgtcgcaaat tcataaaggc gtatcggact tcaatcgtat caatccgcaa tattttcaaa
     4921 gtcttgataa aaaaatggaa tttttatcgc aggaaggttt tgtcccctta ttagaaactg
     4981 tgcggcggga tgtcggacca tcttggtatg cttattttga tttcaatgag acttttgcgc
     5041 gttttgtgca atacttaata gcacgttacg gcgcctataa tattattttc agcggtattc
     5101 atctcgactg gattccgcaa gaattcagtc tcacggcgga gcaatttaac gaggcattga
     5161 cttatcatct ggaaaaatat ggaccgatgc cttttgatca gccggttaca actcttatca
     5221 gcagttcgac ctattcggtg tttggtcatc atcaagattg tccgtggttg acgatgcaca
     5281 gcgtcggtaa taaaccgcgg gaccaccggg tagccgattc gttggaaata atttttcgac
     5341 ttgagccgcc gtacccggcg attaatttcg agccatatta cactggttgg gatcacgaga
     5401 ttaatatgcc gaatggtgaa cgtccgccgg ctaattccga ccgcgataat tactttgcgc
     5461 gagcgcaaat gtacggctcg gtactttcgg gtggtttgtc cggtcacgtt cacgggactt
     5521 cggcttatga cattaccaca actggcgaac cagtcggaaa acgtcatcat atttgggagg
     5581 ccttgaagtt tgaatcggca aattatatga aacacttgag ttcatttatt ttatcagaag
     5641 gaaataaata tcaggattta cggcttgccc gtgaagatat tcatcctaat aaggcaccta
     5701 acagtccgcc aacaggttta gatggctggg cttatatgat gcgaaccccg gataaaaacc
     5761 tggctatgct ttacttcgaa aatcagtcgg ttttgcccac tttgtccggc tttaaaccga
     5821 atacaatgta ccattttaag tggtttgatc cgcgcaatgg tgaatggaaa gaggctcaga
     5881 caatccaagc ggataaaaag ggaaatttga aagtgccact gtttccagac gggaataatc
     5941 cttctatcaa ggactgggcg gctaaaattt gcaaagaaga aaagaataaa taaatttata
     6001 accaaatgta aaggagtatc gttatgaaaa agttagtcat cggcattttg ggtatatttc
     6061 ttatgcagaa tttatacgcc cagaacgtcg aaaataagat tgtcattgat gtcgaggccg
     6121 gccagcatgt gattagtcgg cacatctatg gccatttcgc tgaacatctt ggtcggtgta
     6181 tttacggcgg gatttgggtt ggagaagatt caccaattcc caatactcgg ggcattcgga
     6241 atgacgtggt tgccgccctg aaagacattg ccgcgccgaa tgtgcgctgg ccaggtggtt
     6301 gtttcgccga tgaatatcac tggatggatg gcattggtcc ccgcgaccaa agaccgaaaa
     6361 tgatcaatac ccactggggc ggcgttaccg aagataatag cttcggtacg catgaattta
     6421 tggacttctg cgagcaggtc ggctgtgaac cggtaattac cggcaatata ggcagtggca
     6481 ccgtgcgtga aatggctcaa tgggtcgagt atctcacttc tgataacgtt agtcctatga
     6541 ccgaactacg aaagaagaac ggtcgcgaaa aaccctggcg cgttagattt tggggattgg
     6601 gaaatgaaaa ctggggctgt ggtggcaata tgacggcgga tttctacgcc gatgaaatgc
     6661 ggcgtttttc aacttattgt aaaaattaca gcggcaatga gttatatcga gtagcctgtg
     6721 gcccatctga cgaaaactac gaatggatgg aaaccctaat gaaggaaccg atcaatcgga
     6781 attgctttca gggaatttct ttgcattatt atacggtttg tcatgactgg accaataaag
     6841 gttctgctac caattttaac gaaaaagact ggtttctgac gatgtcgaaa acgctccgaa
     6901 tggatgagtt tgtcaccaaa cactctgcca ttatggataa atatgatccg gaaaagaaaa
     6961 tcggactcat cgttgatgaa tggggtaatt ggcacgatgt cgaacccggc actaatccgg
     7021 cttttctata tcaacaaaat acactcagag atgctctggt tgccgctact aacctgaaca
     7081 tttttaacaa ccactgcgaa cgagtgaaaa tggctaacat tgcccaaatg gtcaatgttt
     7141 tacaggcaat aattctgacg aacgaagacc aaattgtctt gacgcccact tattatgttt
     7201 ataaaatgta caaagtgcat caggatgcaa ctttattgcc gatcgaattg acctgccaga
     7261 attatgaact ggctgacggt tcaattccag cggtgaatgc ctcagcttcc gttgataaac
     7321 agggaattat tcatattacg ctatgcaatc tcgatccgaa taaaaacatg aatctgacct
     7381 gtgaattaaa gagtaaaaac aaattttcgg taagcagtgg gtcaattatc acagctggta
     7441 agatgaactc cttcaatgat tttggtaaac cagaggaagt caatctgcaa aaattcaccg
     7501 attgcaaaat agcccaaaat actctttcag ttaatctgcc agctaaatcg gtggttatgg
     7561 tcgagctggt gagtaaatca gctgataagc cgaagaaata aaagactgat tgtttatcgt
     7621 taaaattggt cggaatcaga caaaaggttg actgaatttt ccgaacgaat atttattttc
     7681 attcggatta atatttataa ttatcaagat tgtcgatctt taaactatca ggcaacaccc
     7741 cgatagtaat aatgaaaaaa gtcagagctt ttgtgcctga ttcgagatga ccgccaacga
     7801 cactgtttgc gtccgacagt gcgatatgag catgaacccg tccattcaga atataacctt
     7861 gcacggaaat tagatccatt ggcactgagg cttttggata ctcttcgtcc ggcggtagat
     7921 ttttactgct aacgacgtgg taatgataat cggtcacggc accgattccg cctaagataa
     7981 cgccattttt gatttttcga ctgctaacgg cttcctttaa tccttctaat aaatccattc
     8041 cagagcgcag gcgaactatt tcaattcgtt caaattcgga agtgtaaatt tgaatatccg
     8101 ctgttttctc ctgagaaaaa cagattgaag accagaaaag aatagtaata atgaacggtg
     8161 attttgccat tttaaaacct ttcaatatta atgagataaa taagcgatta tgacgaattt
     8221 tatcttttta ccagaatggt attatcaact ttaaccaatc tgtttttttg taaaccgatg
     8281 ttaatcaagt tatcgtattg gatttgtgct tgttggatcg aaagtgtccc ttttgaaatt
     8341 tcatccagaa tcccatcggc atcatggtca atggcgacag ctgcttccgg gttgaccaat
     8401 tgttttttgc tgattatttt aaactggttg aacggtttag cattgactgg ataaaaactt
     8461 tttaattcga ggtgaaagtc ttccaggtct tcaatatatt tatgattttc cggagttatt
     8521 tccttgaggc gattttcctt ggtcagcatt tcaaggccat aatcgtaaac tttttgtgcc
     8581 tccgctaaag tggtagtccc gacgctgatt ttatctataa tccgatcctg atcaaagtca
     8641 acagctaaaa aattcttacc aattaattcg ttatagcgct cattattttc aaccgcataa
     8701 atcgagcgaa tgcgatagac ttctccctca aaggtgaatt gatactgagc cacactggtt
     8761 tgcgggcgag tagtgcaatc attaagaagc agtacaatta aaccgagact aaagacaacc
     8821 agaaaagtca aatgaaattg cctacggcga taaatcttta acatgagttt ttctcctttt
     8881 aaaaggtttg ttcttcattt attttattat caaagactaa atcacattca aatttaaagc
     8941 aaatgttttt caaaaaaata taaatatttt acggagaaag ctacctgtaa ttttagccgg
     9001 tgaattagtt agagtgctac aaatagcccg actgatcttt catgggattt taattactcc
     9061 aactttgagt ttgtatataa cttatcgagt ttatcgctaa tcaaagccgg attagtcaaa
     9121 ggcatatcgt cagaatctac ccaatagtta aaaattttaa ccttatcgcg attgctaaaa
     9181 cttggtaaag gactcttatt agttgtcgta atgtggttgc aatttttatt gactttaaag
     9241 gatttaatta ctatgttaat tttatgttat tatgactgga gaagtgggta gagttatgtc
     9301 tcattttagt caaaattcca cttttgaaaa gaagccgaaa cttctcaatc aggtctgttt
     9361 tacccttcgt aataaacact atagccttcg aaccgaagaa gcctacatag gctggatcaa
     9421 aagattcatc ctttttcaca ataaacgaca cccgttacaa acgggtgagg aggagatcaa
     9481 tcaatcctta cgtcagcatc gcaaaaattg aggaaaactc gtgactacga gaagaacaat
     9541 atacccaggt aaccctattt caaaaaaggt ggcaaaaaat atatggagac aaataaaagg
     9601 attttcataa tactgttttt aatgatactt attacaggtt gttataccta taggttttca
     9661 gaaaaacaat tctttaataa taagcaaggt atagagttag attcatgtta tgtaatggaa
     9721 tatgtatttt atagaaatac tgaaaatata acgttagaag gtatcatata tttcaacaaa
     9781 gatgcaacta ttaatgtggt atttttcccg ggtattagtg ggaatcttaa tttatataaa
     9841 tatttagcaa atactttagc acaaaagcta aatgcaaatc tttttatacc aaattatcgc
     9901 ggttatggat atagtgaggg catacccact atgaaaaatt taataagtga tacaaaagat
     9961 cagatagaat atttcctcaa ttatccaaaa gtaaatcgca gattacccct ttttattatg
    10021 ggccattctt ttggaacaaa tattgccttg gagtcggcta aaatggaaaa tatcaaaggt
    10081 gttatattaa tagccccctt ttctacaaac gaggaagtaa tagagcattt aaagaagaaa
    10141 attgtgccct tttatttatg tccatttatt aggatagaac cacaagagcg ccttaaaaac
    10201 attgataact atgaattgct gagagtcatt gataaacctg ttctaatatt gcatggcact
    10261 gatgatgatg aactgccaat atggatgtca gaacatttag tcaacaacct caacaataat
    10321 aaaattgaat taatcaagat atctggggcg ggtcactttg acatatttat taatcaaaaa
    10381 cattctaacc tgaataattc ataaaaataa agatgtttgg ttgtttaata cattgta
//
LOCUS       DAOSMP010000061        10264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_6523_length_10264_cov_11.047703, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000061 DAOSMP010000000
VERSION     DAOSMP010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 10264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10264
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6523_length_10264_cov_11.047703"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PKX09_08670"
     CDS             complement(<1..800)
                     /locus_tag="PKX09_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOU17934.1"
                     /translation="MKLWQKKYDLNATVEKFTVGNDYLLDQELVPFDCQASRAHARML
                     NKIGILSDSETADLLSGIDQILDLHAKGKFKIRPEDEDCHTAIENFLTEKIGIAGEKI
                     HTGRSRNDQVLTALRLYSKTQLNEISSAVQTTIATITEFIQKYGEVKMPGFTHTRKAM
                     PSSVAMWAGALRDALADDLDLVACATKLIDQNPLGTGAGFGVPLALDRQMTTDELHFA
                     RIQENPIYAQNSRGKFEGFALSVMSQIMYDLNKVASDLILFTMPEFNY"
     gene            complement(804..2054)
                     /locus_tag="PKX09_08675"
     CDS             complement(804..2054)
                     /locus_tag="PKX09_08675"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOU17935.1"
                     /translation="MSKKVILAYSGGLDTSVILKWLVNKGYDVICYVANVGQREDFAA
                     VEQKAFKTGASKVYIEDLRREFVNDYIFAALRGNAIYEGRYLLGTSLARPLIAQRQVE
                     IAQMEKTHYYAHGATGKGNDQVRFEFAYYALDTEAIVISPWKDPEFLAEFKGRSDLIR
                     YAEKWNIPVKASLTKSYSEDENLMHISHEAGVLEDPSFRPPENVFSWTVSPQDAPDSE
                     TILEIEFKDGYPIKVTNLNNGVVKEDSLELFYYLNEIGSQNGVGRVDMVENRFVGIKS
                     RGVYETPGATILWTAHRDLEGIAMDKEVMHLRDMLSPKFAELIYNGFWFSPEMDFLMN
                     AFEKSQEKIDGSVTVSIYKGNVTPIGRRSPISLYDQALSSMDIAGGFNANDSLGFIRV
                     NALRLKAHKIVLKKKKPYNWRIKD"
     gene            complement(2132..2605)
                     /locus_tag="PKX09_08680"
     CDS             complement(2132..2605)
                     /locus_tag="PKX09_08680"
                     /inference="COORDINATES: protein motif:HMM:NF013483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HOU17936.1"
                     /translation="MNTKRDRHRAIRQIIASRQISNQNELKDVLEKQGFVTTQATLSR
                     DLMTLKIIKVPDAERGYIYALSENFQSSKPPIEGSSPLQTCQSIAFSGNLAVLKCLPS
                     FAPSVALLLDAYEMNEIIGTIAGDDTVLIILKEGLSHERFRSALVERCPELRNRI"
     gene            2841..5960
                     /locus_tag="PKX09_08685"
     CDS             2841..5960
                     /locus_tag="PKX09_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOU17937.1"
                     /translation="MFQSGRRVRLKLNPSRMGVTTEKTMERAGRLRQQVVFPDGCSFE
                     LISTLEEVSEEYDDPIDLLTNGRIGRVSDLRGNLTYIRLNGRLANLIYSMDTTNTDFY
                     PFQFKPVLNFIDSPTGGLLVADEVGLGKTIEAGLIWTELRSRFEARRLMVLCPAVLQE
                     KWQFELLDKFGIDTQILNSRDTLRSFKEYRSGNRLDFAIIGSMQGLRPHKGWDKDNSE
                     ANDYASLLCRFIKENEFDEPLIDLLIIDEAHYLRNPESMTSKLGNVISNASDYKVFLS
                     ATPIHIKSHDLYQLLNILDADTFYRPAVFDEILKANEPLIKARELLLNYNRKKDVTEN
                     VFSEIKELLISATKHPYLSENRQLAYLIDSFSSEMYLSEKDKRAEFAERLERINLLSH
                     VVNRTRKRDVKEFRVIREAVPERVEMTAAEAEFYYKVTNLIREYARKNLSNEGFLTVM
                     PQRQMSSSMPAALESWINRIEPELEQLDEDTGSSENYTGEIGPLTKILVEQASHLGDL
                     ETLYNDDSKYKRLKHILTNHLRKHPKDKIVLFAYFKSTLKYLERRLSLDGIKCTTLMG
                     GTSYSKYDVIEKFAKPSGPNVLLSSEVASEGVDLQFAYILINYDLPWNPMKVEQRIGR
                     IDRLGQESKKIFIWNLFYNGTIDDRIYNRLYARLKIFEHSLGNLEAVIGEKIQKLTWD
                     LLSQPLTHEEEEEMIIQTENAISNLHVTEERLENEASNLIAHGEYILHQVRAAQELQR
                     TISSRDLWIFVRDFFDQEYEGCEFIQQNANELLFDIKLSKDAKYDLDMYIQRKKLFGQ
                     TKLSNTGQQKVRCLFYNKTIRNTNPNQEIINQFHPLIRFIADKIKLLGKKYYSPVGVQ
                     IDQSNIGNFKKGIYAFAIQLWSLGGIRDIEKLNYEISCISDETNEFSPEQAEKLIVSA
                     ALEGNDWLTIEQEIEIQKAVRLVNTCIEKSENKYIKYVHNVQNENNDRADIQEKAVIS
                     HQKRQLEILNELMKKHQENNRLSLVEATKGKIEKLNSRVEWRLRTIGEQRKLISNNQD
                     VCIGLIKVT"
     gene            6001..7491
                     /locus_tag="PKX09_08690"
     CDS             6001..7491
                     /locus_tag="PKX09_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17938.1"
                     /translation="MNLLKRFFDLFKSKSESEAYALKKILIGAGEGIKPLDKSNVVEP
                     SDVCIIQKPEQVHIVKEEDIEIIIGFDFGTSCSKVILRDAQRNISYAVDFRKYGHKTN
                     TYLLPTRLFLKKENTFDLQDGDILFRDIKINLIDCPSDIIMSDLTNTDVAIAYFCCAF
                     KQIRKWFYDIKGDIYLKNNIIWQINVGIPARDYSDDKLVMLFRYVALLGWELSLDNDE
                     VKISKIHQLISKVPDINNREEGLLSIHPEDIYAIPEIIAAIVGYAKSRMRKDGMYFLM
                     DVGASTVDVSMFNLFKRDGEIKYSILWAELGRYGVLSQLKARLNSLPSEVKDRVILKL
                     NIDQCMDPLPDIFNYLSPDERRSIQDTDSIFTIELRNLIGRVVIKVKKDQNPNDSNWE
                     NGVPIFITGGGSEIKYFQNSILSKFSELSNMNIRYPNIIDLPNLDDLEISDQSLNVIK
                     RFSSAYGLSYRESDIGTIIGAGSIEAISKYEFVVDFSDRYIDKDYM"
     gene            7502..7693
                     /locus_tag="PKX09_08695"
     CDS             7502..7693
                     /locus_tag="PKX09_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17939.1"
                     /translation="MSNDILTLLLELRDLFNLLDNYCRNKQMRLMISQTSIIEWSSIA
                     PWEAVVLSDETYRIGIGKE"
     gene            8103..8318
                     /locus_tag="PKX09_08700"
     CDS             8103..8318
                     /locus_tag="PKX09_08700"
                     /inference="COORDINATES: protein motif:HMM:NF024412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine zipper domain-containing protein"
                     /protein_id="HOU17940.1"
                     /translation="MKACRRRGISRSRFYEYKKRFKIHGLEGLVDLPPIHKTHPQTTP
                     PSIVKKILDMAIRNLMWGCHRLSDQLN"
     gene            complement(8545..9093)
                     /locus_tag="PKX09_08705"
     CDS             complement(8545..9093)
                     /locus_tag="PKX09_08705"
                     /inference="COORDINATES: protein motif:HMM:NF013483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HOU17941.1"
                     /translation="MNTKRDRHRAIRQIIASRQISNQNELKDVLEKQGFVTTQATLSR
                     DLMTLKIIKVPDAERGYIYALSENFQSSKPPIEGSSPLQTCQSIAFSGNLAVLKCLPS
                     FAPSVALLLDAYEMNEIIGTIAGDDTVLIILKEGLSHERFRSALVERCPEKFIQDGTI
                     ESFYKAVGLRMANFGLSMLYIR"
     gene            9396..>10264
                     /locus_tag="PKX09_08710"
     CDS             9396..>10264
                     /locus_tag="PKX09_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOU17942.1"
                     /translation="MPKSIATYILPLAIVIISALIGLLLDRLVFSKWRKNVQKIRPPV
                     LRVVGNSMRGILIIVMGLVGLYFALSYFGIEEEVLNNLRKLLIVLIILTFTILLARIA
                     VGFIENLGRRKKMPSLTLFINLTKIVIYVIGFLIILQTLGVSITPLLTALGVGGLAVA
                     LALQDTLSNLFAGIFIIGTRLVQPDDYIMLSSGVEGFVKDINWRNTIIRTMANNENVI
                     PNSELSKTIITNYSRPDKKMSLIVPVLVGYNNDLNKVEEVLIQTAREVLNEVEGGVKD
                     SEPIVRFNRFNES"
BASE COUNT         3270 a         1776 c         2191 g         3027 t
ORIGIN      
        1 aaataattaa actccggcat ggtgaataaa atgagatcgg aggcaacttt gtttaaatca
       61 tacataattt gcgacattac actaagagca aaaccctcga atttgccgcg actgttctgg
      121 gcgtaaatcg gattttcttg aatacgggcg aaatgtaact cgtcagttgt catttggcgg
      181 tcgagagcta gcggaacacc aaatccagcg ccggtgccga gaggattttg atcaatcaat
      241 ttcgtagcac aggctaccaa atcaaggtca tcggctaggg catctcgaag ggcaccagcc
      301 cacattgcta cgctggacgg catagcttta cgcgtatgcg taaaaccggg catcttaacc
      361 tcgccatatt tttggataaa ctcggtaata gtcgcgatag tagtttgaac ggcagaggaa
      421 atctcgttta attgagtttt gctgtaaaga cgtaaggcgg ttagaacctg gtcattacga
      481 ctgcgaccgg tatggatttt ttccccggct atgccgattt tctccgtcag aaaattttcg
      541 atcgccgtgt ggcaatcttc gtcttcgggg cgaattttaa atttgccttt ggcgtgtaaa
      601 tcgaggattt gatcaattcc actgagcaaa tcggctgttt ccgagtccga gaggatgccg
      661 attttattca gcattcgagc gtgagctcgg gaagcctgac agtcgaacgg tacgagttcc
      721 tgatcgagca ggtagtcgtt gccgacggtg aatttttcga cggtggcgtt gagatcatat
      781 tttttttgcc agagtttcat aggttagtcc ttgatgcgcc agttgtaagg ttttttcttt
      841 ttgaggacaa ttttatgggc tttcagccgg agagcattga cgcgaataaa gcccagactg
      901 tcattagcat tgaaaccacc tgcgatgtcc atactcgaga gagcctgatc gtagagcgaa
      961 atcggcgaac gacgtccgat gggagtaaca ttgcctttgt atatggaaac tgtaactgag
     1021 ccgtcaatct tttcctggct tttctcgaaa gcattcatta gaaaatccat ttcaggtgaa
     1081 aaccagaagc cgttatagat taattcggca aatttaggcg aaagcatatc ccgcaggtgc
     1141 attacttcct tatccatagc aataccttcc aggtcacggt gcgccgtcca taaaatcgtc
     1201 gcgccgggag tttcatagac gccgcgcgac ttaatgccga cgaagcggtt ctcgaccata
     1261 tcaactcgcc cgacgccatt ctgactccca atttcattaa ggtaataaaa aagttccaag
     1321 gagtcttctt tgacgacgcc gttgtttaga tttgtaactt taatcgggta accgtccttg
     1381 aattctattt ccagaatcgt ctcgctatcc ggagcgtctt gtggtgaaac ggtccagctg
     1441 aagacatttt ccggcggtcg gaaagaggga tcttcaagga caccggcttc gtggctaatg
     1501 tgcatcaggt tttcatcttc actgtaagat tttgttaagg aagctttgac gggaatattc
     1561 cacttttccg catatctgat caaatcactg cgacctttga attcggcgag aaattccgga
     1621 tctttccagg gagaaataac aatcgcttcc gtgtcaaggg catagtaagc gaattcgaaa
     1681 cggacctggt cattaccctt gccggtcgca ccgtgagcgt agtaatgggt tttttccatc
     1741 tgggcgatct cgacttgccg ctgggcaatt aaaggacgcg ccagagatgt gcccaataaa
     1801 tagcgccctt cgtaaatggc attgccacgc aaagccgcga aaatatagtc attcacgaat
     1861 tcacgacgca ggtcttcgat atagactttc gaagcgccgg ttttgaaggc tttttgctca
     1921 acggcggcga aatcttcccg ctgaccgaca ttggcgacgt agcaaattac gtcgtagcct
     1981 ttattgacca gccatttgag gatcacagac gtgtcgagac ctccactgta tgctaatatt
     2041 actttttttg acattgatta attcctttca taatagttga ttctaccgga ttgatgtatt
     2101 ttgttaaggt taactttatg aaattaccgg tctaaattcg attgcgtaat tccgggcaac
     2161 gttccacgag cgccgatcga aagcgctcgt gagacaatcc ttcttttaga ataattagaa
     2221 cggtatcatc accggcaatt gtgccgatta tttcattcat ttcataggca tcgagaagca
     2281 gagcgacact gggagcaaaa gaaggcaggc atttgagcac ggcgagattg ccggaaaagg
     2341 cgattgattg gcaggtttgc aagggcgaac tgccctcgat tggcggcttt gaactttgaa
     2401 agttttcgga aagcgcataa atataccctc tttcggcgtc agggaccttg atgattttca
     2461 aagtcattaa atcacgcgaa agagttgcct gagtcgtaac aaaaccctgt ttttcgagaa
     2521 cgtcttttag ttcgttctga ttagaaattt gccgggaggc aataatttgc cggatagctc
     2581 tgtgtcggtc tctcttagta ttcatcttca aacgtataaa tatacttaaa aaacgaataa
     2641 atatacacac taaaaggaca acgcacaagt aaaatttttt cgaaacgggc atcctattta
     2701 tgattggtgc ccattatttg tctgtaaaag agtgaagagt tagagactgg ttatacttct
     2761 gctgaaatat tacttgaacc aattttacag aaaatttagg acttgactca aaagagaaaa
     2821 ataaataaat taaattaaat atgttccaat caggaagaag ggtacgatta aagttaaatc
     2881 ccagtcgaat gggtgttaca accgaaaaga ccatggaaag agccgggcgt ttgcgtcaac
     2941 aggtcgtatt tccagatgga tgttcatttg agttaatatc tactcttgaa gaagtttcag
     3001 aagaatatga tgatccaatt gatcttctaa ccaatggaag aatcggaaga gtaagtgact
     3061 taaggggaaa tctaacatat atccgactaa atggacgatt ggcgaatctg atttatagta
     3121 tggatacaac caatactgat ttttatccat tccagttcaa gccagtactt aattttattg
     3181 actctccgac cggtggttta ttagtagctg atgaagttgg tcttggaaaa actattgaag
     3241 ctggtttaat ctggacagag ttgcggagtc gttttgaagc gcgacggctt atggtgctct
     3301 gtccggcggt tttacaggaa aagtggcaat ttgaactact agacaaattc ggtattgata
     3361 cacaaatatt aaactcaaga gatacattga gatcttttaa ggaatatcga tctggtaatc
     3421 gattggattt cgccataata gggagtatgc agggattacg acctcataaa ggatgggata
     3481 aggataattc cgaggccaac gattatgcat ccctactttg tagatttatt aaagagaatg
     3541 aatttgacga accgctaatt gatttgctga ttattgacga agcacattat ctgcgaaacc
     3601 cggaaagtat gacttcaaaa ctcgggaatg taatctcaaa cgcttctgac tataaggtgt
     3661 tcttatcagc tacccctatt catataaaga gccacgattt ataccaactt ttaaacattc
     3721 tggatgcgga tactttttat agaccggctg tgtttgatga aatcctgaaa gctaacgagc
     3781 cactgattaa agcccgggaa ttgttattga actataaccg aaagaaggat gttacagaaa
     3841 atgtttttag tgaaatcaaa gaactattaa tatcggctac taaacatcct tacctaagtg
     3901 agaaccgaca actagcttat ttaatcgata gtttttcttc agaaatgtat ttgtctgaaa
     3961 aagataaacg ggcggaattt gcagaacgac ttgagagaat aaacttatta agccatgttg
     4021 ttaatcgaac cagaaaaagg gatgtcaaag aattccgtgt tattcgcgag gctgtacctg
     4081 aaagagtaga gatgaccgca gctgaagcag aattttatta caaggtaact aatctgattc
     4141 gggagtatgc caggaagaat cttagcaatg agggattttt aacagttatg cctcagcgac
     4201 agatgtctag ctctatgcca gccgcattgg aatcatggat aaatagaata gaacctgaat
     4261 tagaacaatt agatgaagat acaggaagta gtgaaaatta cactggcgaa atcggtcccc
     4321 ttaccaagat attagttgag caagccagtc atcttggtga tttagaaact ctttataatg
     4381 atgattcaaa atataaacga ttaaagcata ttctcacaaa tcatcttcgc aaacacccta
     4441 aagataaaat tgtgctattt gcctatttta aatcaacatt aaaatacctg gagcgccgct
     4501 tgtctcttga tggcattaag tgtacaacac taatgggcgg gacgtcttat agtaaatatg
     4561 atgttatcga gaaatttgca aaaccatctg gtccgaatgt tcttttatct tctgaggttg
     4621 ccagcgaagg agttgatctc cagtttgctt atatattgat taattacgat ttaccatgga
     4681 atccaatgaa agtcgaacag cgcataggac gaatagatcg actcggtcag gaatctaaga
     4741 agatatttat ctggaatctg ttttataatg gtacaatcga tgatcgaatt tataatagac
     4801 tgtacgcacg tttaaagata tttgagcatt ctttgggtaa tttggaagct gtaattggtg
     4861 aaaagattca aaagctaaca tgggatctat taagccaacc tttgactcat gaagaagaag
     4921 aggaaatgat tattcagact gaaaatgcta tttcaaatct tcatgttacc gaagaaaggt
     4981 tggaaaatga agccagcaac ttaattgctc atggtgaata tatactacat caggtcagag
     5041 cagcccaaga attacagaga acaatttcaa gtcgtgactt gtggatattc gtgcgtgatt
     5101 tctttgatca ggaatacgaa ggctgtgaat ttattcaaca gaatgctaat gaattgctgt
     5161 ttgatataaa gttatcaaaa gatgctaaat acgatctgga tatgtatatc caacgtaaaa
     5221 aattatttgg tcaaacgaaa ttgtctaata ctggtcaaca aaaggttagg tgtctctttt
     5281 ataataagac aattcgaaat acgaatccaa atcaggaaat tataaatcag tttcatccat
     5341 tgattagatt tattgccgat aagataaagc tgttaggaaa gaaatattat tcaccggtag
     5401 gtgtgcaaat agatcaatca aatatcggga atttcaaaaa aggaatatat gcctttgcaa
     5461 ttcaattatg gtctcttgga ggtattagag atattgagaa gttgaattat gaaatatcat
     5521 gtatatcaga tgagacaaat gaattcagtc ccgaacaggc tgaaaaactg atcgtttcgg
     5581 ctgctttaga gggcaatgat tggctaacca tagaacaaga gattgaaatt caaaaagcag
     5641 ttaggttagt taacacttgc atagaaaagt ctgagaataa gtatatcaaa tatgttcata
     5701 atgtccagaa tgaaaataat gatcgagcag acattcagga aaaagcagtg atttcccatc
     5761 aaaaacgcca attagaaata ttaaatgaat taatgaagaa acatcaggaa aataatcgct
     5821 tatcgcttgt cgaagctaca aaaggtaaaa tagagaaatt aaacagccgg gttgaatgga
     5881 gattaaggac tatcggagaa caaagaaaat taatttcgaa taaccaagat gtatgtatcg
     5941 gattgataaa agtgacttag tgattgaagt tgtatataat ttaattccaa aaggtgggaa
     6001 atgaatctac taaaacgctt tttcgattta tttaaatcaa aatccgaatc tgaagcatat
     6061 gcacttaaaa agatactaat aggtgctggt gaaggaataa aaccattaga taaatctaac
     6121 gttgttgaac cttctgatgt atgtattata caaaagccag agcaagttca tatcgttaaa
     6181 gaagaagata tagaaattat tatcggattt gatttcggaa cctcatgttc taaagttatc
     6241 ctgagagatg cccaaagaaa tatctcatat gctgttgatt ttagaaagta tgggcacaag
     6301 actaatactt atctattacc aacacggcta tttttaaaga aagaaaacac attcgacctt
     6361 caagatggtg atattttatt tcgagatatt aaaatcaatt taattgattg cccaagcgat
     6421 attattatga gtgatctgac taatactgac gtagctattg cctatttttg ttgcgctttt
     6481 aaacaaatta gaaaatggtt ttacgatata aaaggggata tatatttaaa aaataacatc
     6541 atttggcaaa ttaatgtagg aatacccgcc agagattaca gtgatgataa actcgtcatg
     6601 ttatttaggt acgttgcatt attaggttgg gaactatcat tagataatga tgaagtaaaa
     6661 atcagtaaaa tccatcagtt aatctccaaa gtacctgata taaataatcg tgaagaaggt
     6721 ttgctttcta ttcatcctga agacatttat gcaattcctg aaattattgc cgcaattgta
     6781 ggttatgcga aatctcgtat gagaaaagat ggaatgtact ttctaatgga tgtcggagct
     6841 tcaacggttg atgtgtccat gtttaatctt tttaaaagag atggtgagat aaagtactcc
     6901 attttatggg ctgagctggg taggtacggc gtgctatcgc aattgaaagc acgcttaaat
     6961 tccttgccaa gcgaggtaaa agacagggta atactaaagt taaatattga tcagtgtatg
     7021 gaccctcttc cagatatttt taattattta tccccagatg agaggcgttc tatacaagat
     7081 actgattcga tatttaccat agagctaaga aacctgatcg gaagagttgt tattaaagta
     7141 aaaaaggatc aaaatcccaa cgattctaat tgggaaaatg gagtgcctat atttattaca
     7201 ggaggcggta gtgaaataaa atattttcaa aattcaatac ttagtaaatt ctctgaatta
     7261 tcgaatatga atataagata tcctaacata attgatttac caaatcttga tgacctagag
     7321 attagcgatc aatctttaaa tgtaattaag cgtttctctt ctgcttatgg gttgagctac
     7381 agagaatctg atattggaac aatcattggc gcaggatcaa ttgaagcaat aagtaaatat
     7441 gaatttgttg tagatttttc tgatagatat attgataagg attatatgta aaggattgat
     7501 aatgagtaac gatattttga ccttactctt agaacttcgg gatcttttca atctattgga
     7561 taattattgc cggaataaac aaatgcgtct tatgatatca caaacatcta ttatcgaatg
     7621 gagtagtatt gctccctggg aagcagttgt tctttcagat gaaacttata gaataggaat
     7681 cggaaaagaa tgataaaaat agacaaaggc gaaatcatca tttatcaacc ggcggataat
     7741 tttccggctt tataagtgag gcttgaggga gaatctatat ggttaataca gaaacagatt
     7801 tcccaattat ttaacaccga acgaagtgtg ataatgaagc aaatcaataa tatctttaat
     7861 accgatgaac tatctaaaga tttagtatgt gcaaattttg cacatactgc cgaagatggt
     7921 aaaaccggac ttgagcgagt atatcgaaga tacttgcgaa gaaaccctga gcgtttataa
     7981 tattcccccc tccgtgtata gttaatcttc ttccgaatca tgagataaga tttaaaacta
     8041 tgctgttgga acggagcgaa tcccgataat tcgggagaag tgaagttcca acagcaggaa
     8101 aaatgaaagc ctgccgccgg cggggaatca gtcgtagtcg tttctacgag tataagaagc
     8161 gttttaagat tcatggattg gaaggcttgg tcgatctgcc accgattcat aaaactcatc
     8221 cccagacgac gccaccatca attgtgaaga aaatcttaga tatggcgatt aggaatctga
     8281 tgtggggttg tcatcgctta tcggatcaac ttaactgacc ggaatatcgg aaagttctat
     8341 ctgcaggcta ttattgatac ctactgcagt tatgctttcg gttatctgca tactggcaaa
     8401 ttaccggaac atttcgtcag tgtgatttat aacgatgttg tgcctcagta tgtattctgg
     8461 aatctaccgg ttaaaacgat attaaccgat aatggcagag aatactgtgg cacgcctaat
     8521 catcactatg agttgttttt aaagttatcg gatatagagc atagaaagac caaagttcgc
     8581 catccgcaaa ccaacggctt tgtagaacga ttcaatcgta ccgtcctgga tgaatttttc
     8641 cgggcaacgt tccacgagcg ccgatcgaaa gcgctcgtga gacaatcctt cttttagaat
     8701 aattagaacg gtatcatcac cggcaattgt gccgattatt tcattcattt cataggcatc
     8761 gagaagcaga gcgacactgg gagcaaaaga aggcaggcat ttgagcacgg cgagattgcc
     8821 ggaaaaggcg attgattggc aggtttgcaa gggcgaactg ccctcgattg gcggctttga
     8881 actttgaaag ttttcggaaa gcgcataaat ataccctctt tcggcgtcag ggaccttgat
     8941 gattttcaaa gtcattaaat cacgcgaaag agttgcctga gtcgtaacaa aaccctgttt
     9001 ttcgagaacg tcttttagtt cgttctgatt agaaatttgc cgggaggcaa taatttgccg
     9061 gatagctctg tgtcggtctc tcttagtatt catcttcaaa cgtataaata tacttaaaaa
     9121 gcgaataaat atacaatccg ccgtacatat atgcaaataa ttttttatga ttgaagcaat
     9181 aaaagtcaac tatcaagatt ttaagtgcat tttagacaat taattgtgtg taattttatt
     9241 tggtactatg atataaagta gagaaagcgt tttttaggat attattcttg aaataaagat
     9301 gcggaatcgt tattattaga ggtaattggg aatgagataa gagagtgccg cgagctgaat
     9361 caaacgtttt tattaaaaaa ttaaaaggat agaaaatgcc caagtcgatt gcaacttaca
     9421 ttttaccatt agcaatagta ataattagtg ccttaatcgg attactgctg gataggttag
     9481 tgtttagtaa atggaggaaa aacgtacaaa aaatcagacc cccggtgtta agggttgttg
     9541 gtaattcaat gcgtggcatt ttgataatcg tgatgggatt ggtgggcttg tattttgctt
     9601 taagttattt tggaattgag gaagaagttc taaataactt gcgcaagtta ctaatagtgc
     9661 tgattatttt gacgtttacc attttgctgg cacggattgc ggttggtttt atcgaaaatc
     9721 ttggtcgccg taaaaaaatg ccttcgctga ctctctttat taacctgaca aaaattgtca
     9781 tctacgtaat aggatttttg ataattctac aaacactagg tgtttcaatt acgccattgc
     9841 taacggcttt aggagtcggc ggcttggcgg tggcattggc actacaggac acgctttcta
     9901 atctttttgc cggtattttt attatcggta cgaggttagt tcagcccgat gattatatta
     9961 tgttatcgag cggggtagaa ggatttgtta aagatatcaa ctggcgcaat acgattatcc
    10021 gaactatggc gaacaacgag aatgttattc ctaattcaga gctgtccaag acaattatca
    10081 ctaattatag ccgtccagat aaaaaaatgt ctctcatagt tccggtattg gtcggctata
    10141 acaatgatct aaataaagtt gaagaagttt taatccagac tgccagagaa gttttaaacg
    10201 aagttgaggg aggtgtaaaa gatagcgaac ctatcgtgcg ctttaatcgt tttaacgaaa
    10261 gtag
//
LOCUS       DAOSMP010000062        10119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_6645_length_10119_cov_15.909678, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000062 DAOSMP010000000
VERSION     DAOSMP010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 10119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10119
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6645_length_10119_cov_15.909678"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1412
                     /locus_tag="PKX09_08715"
     CDS             <1..1412
                     /locus_tag="PKX09_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOU17943.1"
                     /translation="LNKQITQLSTAEIINQIVELTNFTALLAGLPDGTQKIANVKKLI
                     DLALEWEKSESLSPIDFIRRIRIYRDKEVREGEANLSAAKGDAVTIMTIHAAKGLDSQ
                     IIFLPLLANGINYRSNHFLFHSDIGAAVAIKADKNQPHPFHYNQLKHLERRRTLAEEK
                     RLLYVAMTRARSYLVCSATVSGKSDEPDKSLWDLSAEIFVRAHEKNLCRIVKLTKTEI
                     EQTALPSGISLVQPVELNDETLRRIRTQIQPLEFKPPIRNLTATALAEWVVAGEKIAS
                     TAEPEDDQALSPQELGTLIHKAFSWWDFQSLDSFQNMLADLLRPYRLTAPESQKISAE
                     LSDWVKQLLDSDNHLRQRLTSARNRRREVEVVGLYGEMILEGKIDLLIENDDNTLTII
                     DFKSDRIAEKPDEVLLKKYSTQLDFYAFILERSSGRKVREQALYFIRNGLLITKTITE
                     SVLDEAEQNIRKYISELAK"
     gene            complement(1480..2169)
                     /locus_tag="PKX09_08720"
     CDS             complement(1480..2169)
                     /locus_tag="PKX09_08720"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HOU17944.1"
                     /translation="MEDNRNKIELMRPAITITIIAVIMMLIASAISWQKLPNDVRVPV
                     HWNIAGKADRYGSKFQGLMLMPLFTIFLAVCMVVVPLIDPHYRHIKSSMKAYNIILSA
                     IALLMTFLHFLILMATFGQPIDIMKNVCLAIGLLFILIGNYTPKIRSNYFLGIRTPWT
                     LSSELSWHKTHCFAGRLFILLGALIGLGGLTLPGIAVFVILLVGVIGLLLSVTIYSYV
                     IWQKDPEKRKV"
     gene            complement(2150..2437)
                     /locus_tag="PKX09_08725"
     CDS             complement(2150..2437)
                     /locus_tag="PKX09_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HOU17945.1"
                     /translation="MSTDYRALSDPTRRQILKLLREKDLTAGEIAEHFTMTKPSLSKH
                     FNILRSAGLIQGEKSGVTITYHLNVSVLEEAIMALMEIFKLKGENNGRQSE"
     gene            2631..2706
                     /locus_tag="PKX09_08730"
     tRNA            2631..2706
                     /locus_tag="PKX09_08730"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2667..2669,aa:Pro,seq:tgg)
     gene            2730..2803
                     /locus_tag="PKX09_08735"
     tRNA            2730..2803
                     /locus_tag="PKX09_08735"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2764..2766,aa:Arg,seq:tct)
     gene            3158..5323
                     /locus_tag="PKX09_08740"
     CDS             3158..5323
                     /locus_tag="PKX09_08740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOU17946.1"
                     /translation="MSKLSSAEIENIISLLKEGKPLPEEYKTILFDTNLAGRPTGKEY
                     ELVYADKKREVDILSDTMAVPLQPVKTFRNGLTASQASKNGNDWSNMIIFGDNLQVLK
                     TLLQLKQEGKLKNADGTPGVRLVYIDPPFATKQEFRGSQDQKAYQDKIAGARFLEFLR
                     ERLVFLRELLSEDGSIYVHLDWKKGHYVKVLMDEIFSETNFKNEIIVKRGQKNVQAQF
                     TTIDSLASGHDTLLFFSKQSGTRFSILKSSLDEMKPGAWNNHWRGTDRPAMRYELFGI
                     LPDRGQWRWSKERTMKAVENYERYVNQYSKTMSIDEYYVHILETTGEKLNFVRLSSTR
                     KPEHYVPPTEHQILSDVWMDVTAYEARKDYPTQKSEELLSRIICGFSNPSDLVLDCFA
                     GSGTTLAVAEKLGRRWIGVDCGKLAIYTMQKRLLNIAESKDLEDSQKKKKYGKPCKPF
                     TLYNAGLYDYKMIKELPWEQYRDFALKLFQCRDERHEIARIELDGYLGADSVMVFNYQ
                     KYPDAVLDRGFIDDLHEYLGDRIGKRFFIIAPAASVQFLEDYIEKGNTKYYILRIPYS
                     IIEEIQNRGFSKIKQPASEMDVNDTVEAVGFDFIQTPMVECKYCLDNRKGQLDFGKGS
                     RDCVIRIEEFESRVISRKPLDFANLETLSMVMLDYDFDGEVFDLDEVFYAEELKKNNY
                     ELRFAEDKVKDKIMIIYIDIFGNEKREIKSLKDFKKGIS"
     gene            5325..6497
                     /locus_tag="PKX09_08745"
     CDS             5325..6497
                     /locus_tag="PKX09_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOU17947.1"
                     /translation="MEINTNTQNYSTLITDLTSLIEQGRQVAVRYVNTALVTTYWLIG
                     RRIVEFEQKGKERAEYGAALLKHLSDDLTKQFGKGWGEPNLRAIRQFYMIYGDIGKRY
                     TVCSESTKSDGTIIRHTSCSELVPSQEIPSELQTLSIETFHNLFPLSWSHYRLLMRLD
                     EPFKREFYEAECIRGNWSVRQLDRQIQSLLYERTALSRRKLAVIAKAHKNPIILKPED
                     EIKDPYILEFLGLKDEYSESQLEEALIRHLEHFLLELGVGFTFVARQKRIDLEGSHYR
                     LDLLLYHRILKCLVAIDLKIGEFGYTDAGQMNLYLNYLKDKEKFPEENDPVGLILCTD
                     KKKTVVEYALGGMSNRIFASKYKLQLPDPEVLEAEITREKQRLLEMKIAGEEIKIK"
     gene            6500..8998
                     /locus_tag="PKX09_08750"
     CDS             6500..8998
                     /locus_tag="PKX09_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOU17948.1"
                     /translation="MLEKQTFLNKDLVLRVSPSYDPRKFEPNKYEAFLDALCGNREYQ
                     KEAIRITLRYFLGGEYQSLRELAEENYHSNPKLQEKYSSFEDFSRVLQLPDQLSGSLD
                     HATATGKSYVMYGIARIMLAEGAVDQVLVLCPSNTIEAGLKEKFLSLSADKTLKDLLP
                     SDSIVANPRIINASNTIQRGDICIENIHATYINTKSAIEDSLVGKGERTLVLNDEAHH
                     LMNPSDTALKKWKEFLLDSKYGFKFIVNVSGTCYIGSEYFTDVIHRFSLKNSIEEKFV
                     KSIRYVAEDSSGSEDEKFQKIYENHIENKTVKYREVKPLTILVTKDIAACKRLTEKLT
                     TFLAEKENISKEQAAEKVLIVTSANEHKNNIPILRRVDDRDNPIEWITSVSMLSEGWD
                     VKNVFQIVPHEERAFNSKLLIAQVLGRGLRIPPVYKGEQPIVTVFNHDKWSSNIKHLV
                     DEVLEIEKRLYSYPVEKKGDYNFDLYNINYKRTEEKVEYPQENPYELLKKEYIAYSSQ
                     AEVVPEDTVYVTAINRARETVTYSITHKLYSVEEIAQDVFNRLLIFDQEAETEYSQKF
                     TKQKIAKIIRKSLAEIQDESGKVNETNRIATLKAFGVIKRKGTKSLRFKIEAQKLLKI
                     STKEIRKNSLGVGSLRHDSTVFWDDYSITLGESADIKLLKEIEEDESLPRSAIIKVEN
                     KYNFKTPLNVAFASHEPERRFIRGLTSDKVSRTINAWIKSLDVGFYSIDYSWRKGEHP
                     KQGSFNPDFFIKIGQDILVIEIKSDDDISDENRAKLRYAREHFARVNDLQKEQRYYFK
                     FLSPASFELFFQMLSNGTYQDFKSELEAKLENRD"
     gene            9240..>10119
                     /locus_tag="PKX09_08755"
     CDS             9240..>10119
                     /locus_tag="PKX09_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOU17949.1"
                     /translation="MFGKGIRRSFFGLSVLLVAMAYGDSNIVINPGFEDGKEGWFDRT
                     CAIEIVTSPVYEGRYSGKAFQRLANWQGIKQSVFGRMVDGKTYQVSGWVRLDNAPSDE
                     VAISFEQQDDSGTKYIGVARGIATDTAWTKLSGEFTLNVTGTLSVLDVYFEGPAPGVN
                     FYVDDVVVYGPEANPPVIIPPMPQSKSQVDAKIRHQIIEGFGASGAYYTMNLVNHNKK
                     SELYDLLFKELGLDIFRIRNNYDMEPNTMQETVEIIKNAKALRGDNLKILMSSWSPPA
                     YLKSNNNVVGGTLKKVA"
BASE COUNT         3041 a         2069 c         2298 g         2711 t
ORIGIN      
        1 aattaaacaa acagattacg caactatcca ccgccgaaat aattaatcaa attgtcgagc
       61 tgactaattt taccgcgctt ctggccggtt taccggacgg cactcagaaa attgccaacg
      121 taaaaaaatt gattgacttg gcgttggaat gggaaaaatc tgaaagcctc tcgccgattg
      181 attttatccg gcgtattcgt atttatcgag ataaggaagt acgcgaaggc gaagccaatc
      241 tctccgccgc caaaggtgat gcggtaacga ttatgacgat tcacgcggca aagggtcttg
      301 acagtcaaat tatctttctg cctttgctag ctaatgggat caattaccgc agtaatcact
      361 tcctttttca ttctgatatc ggtgcggcgg tcgccattaa agccgataaa aaccaacctc
      421 atccgttcca ctacaatcaa ttaaaacatc tcgaacgccg ccggacttta gccgaagaaa
      481 aacgcctgct gtacgtcgct atgactcgcg cccgttcgta tttggtctgc tcggcaactg
      541 tatccggcaa atccgacgaa ccggataaat cgctctggga tttaagtgcc gaaatttttg
      601 ttagggcgca tgaaaaaaat ctgtgccgca tcgtaaaatt aaccaaaacc gaaatcgaac
      661 agacggctct ccccagtggg atttcactcg ttcagccagt cgaattaaac gatgaaacgt
      721 tgcggagaat tcgcactcaa attcaaccgc tcgagttcaa accgccgatc cggaatttaa
      781 ctgctaccgc tctggcagaa tgggtagtcg ccggtgagaa aatcgcctcc accgccgagc
      841 cagaagacga ccaggcgctt tcaccacagg agttaggcac tttgattcac aaagccttca
      901 gctggtggga ttttcagtcg ctggattcgt ttcaaaatat gctggctgat ttactgcgtc
      961 cctaccgact aaccgcacct gagtcgcaga aaattagcgc tgaactttcc gactgggtta
     1021 agcaattgct cgattctgat aatcatttac gccagcgatt gacttccgcc agaaatcggc
     1081 gacgggaagt cgaggtggtc gggctttacg gagaaatgat cctcgagggt aaaattgatc
     1141 tgctcatcga aaatgacgac aatactctta ctattataga cttcaaatcc gaccgcattg
     1201 cagagaaacc ggacgaagtt ttattgaaaa agtactccac ccagctggat ttttacgctt
     1261 tcattctcga acgcagttct gggcggaaag ttagagaaca ggctctttat ttcattcgca
     1321 acggtttatt gattactaaa acaataaccg agtcggtatt agacgaagcc gaacagaata
     1381 ttcgcaaata tatcagcgag ttggcaaagt aagtattttt aacgactcaa gttacctcca
     1441 agaaaataaa aaggtgatct tcttgatgcg ttttgcggtt tataccttcc gcttctcggg
     1501 atctttttgc cagattacat aggaataaat cgttacagat agaagcagtc cgattactcc
     1561 aactagcaga ataacgaaaa ccgctatgcc aggcaaagta agtccgccta agccaatcag
     1621 cgcccccagt aaaataaaga gtcgccccgc aaaacagtga gtcttgtgcc aggacaattc
     1681 actggataaa gtccatggag ttcgtatgcc gaggaaataa ttactacgaa ttttaggggt
     1741 ataattaccg attagaataa ataatagtcc aatagctaaa cacacgtttt tcataatgtc
     1801 tatcggttgc ccgaacgtag ccattaatat caagaaatgc agaaatgtca ttagcaatgc
     1861 aatggctgac agaattatgt tgtatgcctt catagatgat ttgatatgcc gataatgcgg
     1921 atcaatcaat ggtactacaa ccatacaaac tgccagaaaa atcgtgaaaa gcggcatcaa
     1981 catcagtcct tgaaatttac tgccataacg atcggcttta ccggcgatat tccaatggac
     2041 aggaacgcga acatcgttag gtaatttctg ccaagagata gcggaggcaa ttaacatcat
     2101 aattactgcg ataatcgtaa tggttatggc cggtcgcata agctcgattt tattccgatt
     2161 gtcttccatt attttcccct ttgagtttaa atatttccat caatgccata atggcttctt
     2221 caagtacact gacattcaaa tggtaagtaa ttgtaacccc cgatttttca ccttgaatca
     2281 gtccggctga ccgtaaaatg ttaaagtgct ttgacagaga gggtttagtc atggtaaaat
     2341 gttcggcgat ttcaccagcg gtcaggtctt tctcgcgcag taatttcaga atttgtcgcc
     2401 gggttggatc ggatagcgcc cgatagtcag tactcatttc ctttttaaat tccctattaa
     2461 tgctaagtat taatctttat ttaggtaaga ggcaaacaat tttttcaggc ttgactttaa
     2521 tgactttaat atcctaactt tacgtgcttt tttttatcca ttgataatca agttcattat
     2581 aataacttaa agtgattgtt ttttcataat cagagataga atttggagaa cggggtgtag
     2641 cgcagtccgg tttagcgcac ctgctttggg agcagggggc cggtggttca aatccactca
     2701 ccccgacgat ctttctgatt tttactaatg ccccgatagc tcaagtggat agagcatctg
     2761 ccttctaagc aggtggttgc aggttcgagt cctgctcggg gtacgcgcat attttactta
     2821 tccattatta acgttaaata tttcaaatga tagagcattg accttctaag caggtgatta
     2881 caagttcttc cgcaggaatc ccgaaacttc gggagagtcc taattggagt gctattttat
     2941 tcaaaaggta gaattccttt cgtaactatt tctacttttt ctgttgtaat aagggaaaag
     3001 gggggcttgt aaactttttc cttgaaattc tcaagccata ttcatataat catagaagca
     3061 cagttaaacc aaggtagtga aatgctgaag ttaggattga aatgaaattg agaaaatccg
     3121 gctggaatgc acccaggata aagcgagggt aatagctatg agtaagcttt catcggcaga
     3181 aatcgagaac attatcagcc ttctcaaaga aggcaaacct ttgccggagg agtacaagac
     3241 gattctcttc gataccaacc ttgccggtcg gccgacaggg aaggaatacg aactcgttta
     3301 cgctgacaag aagcgggaag tggacattct gtccgataca atggccgtgc ctttacagcc
     3361 ggtcaagacc ttccgcaacg gcttaaccgc aagtcaggcg agcaagaatg gcaacgactg
     3421 gtcgaatatg attatattcg gtgacaacct tcaggtcttg aaaactctct tgcaattgaa
     3481 acaggaagga aaacttaaaa atgccgacgg tactccgggg gtgaggctgg tttatattga
     3541 tccgccgttt gccaccaaac aggaatttcg gggcagtcag gatcagaagg catatcagga
     3601 taaaattgcc ggagcaagat ttttggagtt tttgagggaa cggctggttt ttttgaggga
     3661 attgctgagc gaggatggga gtatttatgt gcatttggat tggaagaagg ggcattatgt
     3721 aaaggtttta atggacgaaa tatttagtga gactaacttt aagaatgaga taatagttaa
     3781 gcgcggacaa aaaaatgttc aagctcaatt tacgactata gattcattgg caagtggtca
     3841 tgatacttta ctcttttttt caaagcaatc gggaactaga ttttcaatat taaaatcatc
     3901 acttgacgag atgaagcctg gagcatggaa taatcactgg cgtggaactg atagacccgc
     3961 tatgcgatac gagctatttg gaattttacc ggatagaggc caatggcgat ggtcaaaaga
     4021 aagaacaatg aaagcagttg aaaattatga acggtatgta aatcagtatt ctaaaacgat
     4081 gtctattgat gaatattacg tacatatttt agagacaact ggagaaaaac tcaactttgt
     4141 ccgtctttca tcaactagaa aaccagaaca ctatgtaccc cctacagagc atcaaatttt
     4201 aagtgatgtt tggatggatg tcactgctta tgaagctaga aaggactatc ctacacaaaa
     4261 aagtgaagaa ttgctgagta gaataatttg cggtttttcc aaccccagcg accttgtgct
     4321 ggattgcttt gccggttcag gtactaccct ggctgtggcg gaaaaacttg gcagacgctg
     4381 gatcggggtg gattgcggga agttagccat ttacactatg cagaagcggc tcttgaacat
     4441 cgctgaaagc aaagacctgg aggattcgca gaagaagaaa aaatacggca agccttgtaa
     4501 gccttttacg ctttacaatg ccggtctcta cgattataag atgatcaagg aactcccctg
     4561 ggagcagtat cgggattttg cccttaagct ctttcagtgt cgcgatgaac ggcacgagat
     4621 tgcccgcatc gaactggacg gctacctcgg ggcggacagc gttatggtct tcaactatca
     4681 gaaatatcct gacgccgtac tggatcgggg ctttattgac gatcttcacg aatacctggg
     4741 agaccggatt ggcaagcgtt tctttatcat cgctccagcc gcctcggtgc agttcctcga
     4801 ggattatatc gaaaagggta atacgaaata ttacatcctg cgcattccct attccattat
     4861 cgaggaaatc caaaaccgcg gttttagcaa aatcaaacaa ccggcgagcg agatggatgt
     4921 caatgatacc gtggaagcag taggttttga ttttatccag acaccgatgg ttgaatgtaa
     4981 atattgcctc gataaccgga aagggcaact ggactttgga aaaggatcga gggattgtgt
     5041 tatccgaatt gaggagttcg agagccgggt catttcccgc aaaccgctgg acttcgctaa
     5101 tctggagacg ctttcgatgg ttatgctgga ttatgatttc gatggtgagg tctttgatct
     5161 ggatgaggtt ttttacgccg aagaattgaa gaaaaacaat tatgaactgc gctttgccga
     5221 agataaagtg aaggataaga ttatgattat ctacattgac atctttggaa atgagaagcg
     5281 agagataaag agcttgaagg attttaagaa aggaatttct taaaatggag atcaatacaa
     5341 atactcaaaa ctattccact ctcattaccg atcttacttc tctcatcgag cagggacgcc
     5401 aggtagcagt tcggtatgtg aatactgcct tggtaactac ttactggctc atcggccgca
     5461 gaattgtgga gtttgaacag aaagggaaag agcgggcgga atatggggcg gctttgctca
     5521 agcatctttc tgacgatcta accaaacaat ttggcaaagg ttggggcgaa cctaatttga
     5581 gagcaattcg acaattttat atgatttatg gagatattgg aaaacgctac acagtgtgta
     5641 gcgaatctac aaagtctgat ggaactatta tccgacacac atcgtgtagc gaattggttc
     5701 cttcccaaga aattccatca gaacttcaga cattgtctat agaaaccttc cataacttgt
     5761 ttcctctttc atggtctcac tatcgcctgc ttatgcgtct tgatgagcca ttcaaaaggg
     5821 aattctacga agccgaatgt attcgcggca actggtcggt caggcaattg gaccgccaga
     5881 ttcagtcctt gctctatgaa cgaacggcat tgtccagacg caagctggcc gtcatcgcca
     5941 aagctcacaa aaaccctatc attctcaaac ccgaggatga aatcaaagat ccctatattc
     6001 tggaattcct cggtttaaaa gacgagtatt ccgaatcaca attggaagag gctttaatca
     6061 ggcatctgga gcattttctt cttgaattgg gagtgggttt cacctttgtc gccaggcaaa
     6121 aaaggataga cctcgagggg agtcattatc gtctcgacct tcttctttat caccgaatat
     6181 taaaatgtct tgtggcgata gacttgaaga tcggtgagtt cggttatacc gatgcggggc
     6241 aaatgaacct ttacttgaat tatctgaaag acaaagaaaa atttcctgaa gaaaacgacc
     6301 cagtaggctt gatcctctgc accgataaaa agaaaacggt agttgagtac gccctgggtg
     6361 gaatgagcaa caggatattt gcctcgaagt ataaattgca gttgcctgat cccgaagttt
     6421 tggaagctga aataacgcgc gaaaaacaga gattgctgga aatgaaaatt gctggtgaag
     6481 aaataaagat aaaataacca tgcttgaaaa acagacattt ctaaacaaag atttagttct
     6541 tcgagtatct cccagctacg acccccgaaa gtttgagcca aataaatacg aggcttttct
     6601 ggatgccctc tgcggaaaca gggagtatca gaaagaagca atccggatta ctttgcgcta
     6661 ttttctgggc ggagaatacc aaagcctcag agagctggca gaggaaaatt atcacagtaa
     6721 tcccaaactt caggagaaat attcgtcttt cgaggatttc agccgcgtct tgcaactgcc
     6781 ggatcaactc tccggttctc tggatcatgc cactgccacc ggcaagagtt atgtaatgta
     6841 tggtattgct cgcattatgc ttgccgaggg cgctgtggat caggtgctgg ttctttgtcc
     6901 atctaatacc atcgaagctg gtttaaagga gaagtttctc agcctctccg ccgataaaac
     6961 gcttaaagac cttcttcctt ccgactccat agttgctaat ccccgcatta tcaatgcctc
     7021 caataccatt cagcgaggcg acatctgtat cgagaacatc cacgctactt atatcaatac
     7081 gaaatccgct atagaagaca gcctggtcgg taaaggtgaa cggactctgg tactgaacga
     7141 tgaagcccac catttaatga atccttcgga tacggcgctt aagaaatgga aagaattttt
     7201 actcgattcc aaatacgggt tcaagttcat tgtaaatgtt tccggcacct gctacatagg
     7261 gtctgaatat tttaccgatg tcatacaccg tttttccttg aagaattcca tcgaggaaaa
     7321 gttcgtcaaa tctatccgtt atgtggcgga ggattcatcc ggaagtgagg acgagaaatt
     7381 tcagaagatt tacgaaaatc atattgagaa caagacggtt aaataccgtg aggttaaacc
     7441 gcttaccatt ctggttacca aagacattgc ggcctgcaaa cgcctcaccg agaaactgac
     7501 tactttcctg gccgaaaaag aaaatatcag caaagagcag gcggcagaaa aggttttgat
     7561 cgtaacctca gctaacgagc ataaaaataa tattcccatc ctgcgacggg tggatgacag
     7621 ggacaatccg atcgaatgga ttacctccgt cagcatgctt tccgaaggtt gggatgttaa
     7681 aaatgtcttt cagatcgtac ctcacgagga gcgagcattt aattccaaac ttcttattgc
     7741 tcaggtttta ggacggggtc ttagaattcc gccagtctat aagggcgagc aacctattgt
     7801 tacggttttc aaccatgata aatggtcaag taatattaaa catctcgtgg atgaagtcct
     7861 ggagattgaa aaacgcttat attcctaccc ggtcgaaaag aaaggggatt ataattttga
     7921 tctttataac attaattaca agcgaaccga ggagaaagtg gaatatcctc aagaaaatcc
     7981 ttatgaactc ctgaagaaag aatatatcgc ctactcaagt caagcagaag ttgtgccgga
     8041 ggatactgta tatgtaactg ccattaatag agcaagggaa accgtaacat actccattac
     8101 gcataaattg tatagcgtcg aagaaatcgc gcaagatgtc ttcaatcgcc ttctgatttt
     8161 tgatcaagag gccgaaactg agtacagcca aaagttcact aagcaaaaga ttgcgaaaat
     8221 catcagaaaa tctcttgcgg aaatccaaga tgaatccggc aaggtaaatg agacaaaccg
     8281 gattgctacc ttaaaggcgt tcggtgtgat taaaagaaaa gggacaaaga gcctaagatt
     8341 caagatcgag gcccagaaac tactcaaaat aagcactaaa gagattagaa aaaattcctt
     8401 aggagtgggg tctcttcgcc atgatagtac ggtgttttgg gatgactact ctataactct
     8461 tggagaatcg gctgatataa agttgctgaa agagattgag gaggatgaaa gccttccaag
     8521 atcagctata atcaaagtcg agaataaata caatttcaag actccgctta atgtggcttt
     8581 tgcttctcat gagcctgagc gcaggtttat ccggggattg acatccgata aggtctctag
     8641 gacaataaat gcctggatca agtctctcga tgtcggtttt tatagtattg attactcctg
     8701 gcgcaagggc gagcatccca agcagggaag tttcaatccg gatttcttta tcaagatcgg
     8761 acaagatatt ttggtaattg aaataaagtc ggacgacgat atttccgacg aaaatagggc
     8821 taaactgcgc tatgccagag aacactttgc cagagttaac gatttacaaa aagagcagag
     8881 gtattacttc aagttccttt caccggcaag tttcgaatta tttttccaga tgttgagcaa
     8941 cggcacttac caggatttca agtccgagct tgaggcaaag ttagaaaaca gggattagtt
     9001 gattttcatc ctttgctttt gcaccctagc cgggagctta actctttacc agaatcacct
     9061 ttctattaag ataaatgaag gcttgtgatc acgagatagg ctttagcttt aacaggaatc
     9121 ccgactactc ggagattttt atccggttag ttttgattac ttaaaataag tttttaaaat
     9181 tggcaataaa ttgtagattt ctgttcatta tgaaattcta actaaggagt attagcaaaa
     9241 tgtttgggaa aggaattcgc agatcatttt tcggattgag tgttctgctg gtagcaatgg
     9301 cttatgggga tagtaacatt gtaattaatc ccggatttga agatggaaaa gagggttggt
     9361 tcgatcgcac ttgcgccata gaaattgtaa cctcgccggt atatgaaggt cgctatagcg
     9421 ggaaagcttt tcagcgcctg gctaactggc agggtatcaa gcagtcggta tttggcagga
     9481 tggttgacgg caaaacctat caagtttccg gctgggtcag attagataat gcgcccagcg
     9541 acgaagtcgc catttctttt gaacaacaag acgacagcgg aacaaaatat atcggggtcg
     9601 ctcgcgggat agccaccgat accgcctgga ctaaactttc cggtgaattt acattaaatg
     9661 tgactggtac tttgtcggtt ctggatgtgt atttcgaagg accggcgccg ggcgtcaatt
     9721 tctacgtgga tgatgtagtc gtatatggtc cggaggcgaa tccaccggta atcattccac
     9781 ccatgccgca aagcaaaagc caggtggatg ccaaaatccg ccaccaaata atagagggct
     9841 tcggcgcttc cggtgcttat tatacgatga atctggtaaa tcataataag aagtccgaat
     9901 tgtacgatct acttttcaaa gaacttggtc tggatatttt ccgcattcgc aataattatg
     9961 atatggaacc gaatacgatg caagaaaccg tcgagattat caaaaacgct aaggcactgc
    10021 ggggagacaa tttgaaaatt ttgatgtctt cgtggtctcc accagcttat ttgaagagta
    10081 ataacaatgt ggtaggtgga acgctcaaaa aggtcgccg
//
LOCUS       DAOSMP010000063         9969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_6757_length_9969_cov_16.243898, whole genome shotgun sequence.
ACCESSION   DAOSMP010000063 DAOSMP010000000
VERSION     DAOSMP010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 9969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9969
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6757_length_9969_cov_16.243898"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="PKX09_08760"
     CDS             complement(<1..158)
                     /locus_tag="PKX09_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU17950.1"
                     /translation="MTIKDIARLANCSLSTVSKALNGRQDVSEATRQRIMQIVAEYNF
                     TPSAYGKG"
     gene            complement(638..1639)
                     /locus_tag="PKX09_08765"
     CDS             complement(638..1639)
                     /locus_tag="PKX09_08765"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOU17951.1"
                     /translation="MKKYKISNDKGVSAEIISYGAIVVSLTTPNRNGQLEDIILGFDD
                     LASYEKDNTFQGAIVGRYGNRIAGGKFSLDGKVYQLNLNDGPNHLHGGPKGFFKVIWD
                     IEQLDSQSVRLTYVSPDGEKGYPGEVKISVVYTITDDDALRIDYYGTTDRPTILNPTS
                     HCYFNLSGSPKNTILDHELMLNADFFTPIDEFSIPTGEIRPVVGTPLDFRVPKPIGRD
                     IAAEDEQIKFGKGYDHNWVINGMPGQVRLAATVYESKSGRLMECLTDQAGLQFYSGNF
                     LDGSLIGKGGIRYNYRCALCLEAQAFPDSPNKPHFPSVVLRPGEEYRQTTIYRFSVR"
     gene            complement(1688..3274)
                     /locus_tag="PKX09_08770"
     CDS             complement(1688..3274)
                     /locus_tag="PKX09_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOU17952.1"
                     /translation="MFKLFAGLDVSTQSTKLVIIDLAAKKVVYVNSVNYDTDLPHYNT
                     KNGAIQGLGEGVSESDPRMWIEAVDRVFANAVAAGIDMSQIGCIAVSGQQHGLVALDA
                     QGNLSRPTSKLWNDFSTLEECEILTEKVGGLEQMIAEVGNSQRTGYTAAKIFHMVRHE
                     PEIYHKTATLFLVHNYINYYLTGGVRVMEPGDTSGMALWNPKNGQWSQKVIQAIAPDL
                     MSKLPEVKPSDQTIGYISPELVAKFGFSKDCLIDAGCGDNMYGAVGTGNVEPGIVTVS
                     LGTSGTAYTFMEEPYIDPTGEIAAFCDSTGHYLPLLCVSNLANGYNEFIKLHKMTHQV
                     FDKMLDETPPANNGKLLIPWYVGERTPDLPLAAPIYFGFGLADFTKANLARAIIEGHI
                     LNLYTGFKRMPVDVKEIRLTGGLSRSDTWCQTIADIFEAQTVPVEGEGAALGAALHAA
                     WTWSKAQNKEVSLKELVKPFVILDEKRRKQPIEANLPVYRKLSAMFESISCRIRGLKG
                     DDPFRLRAELIKLSKTKTNS"
     gene            complement(3298..4638)
                     /gene="xylA"
                     /locus_tag="PKX09_08775"
     CDS             complement(3298..4638)
                     /gene="xylA"
                     /locus_tag="PKX09_08775"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOU17953.1"
                     /translation="MSKQVLIGQREYFKGIGKIPYEGPESKNPLAFKYYDENRVVAGK
                     KMKDHFRFAIAYWHTFCNNGGDPFGAEGTVDFPWNESKDPMQAAKDKLDAAFEFFTKL
                     GTPFYCFHDRDMAPEGKNVVESEKNLKELVKIAKAKQEESGVKLLWGTANVFGHKRYT
                     NGAATNPNFYVIPHVAAQVKAAIDATIELGGENYVFWGGREGYYSLLNTNMKREIEHM
                     AMFLTKARDYGRASGFKGTFLIEPKPMEPTKHQYDFDSATVIGFLNKYGLQNDFKINI
                     ENNHATLAGHTFAHEVQTACDAGLFGSLDINQGDPFNGWDTDEFLHNLYDATELMLIL
                     LQNGGIGHGGMNFDAKNRRSSTDLEDIFIGHIYSMDTLARGLLIANDILEKSDYLKMK
                     KERYSSFDSGDGARFEKGELGLVELAKIAEEIGEPKMISGKQELYESLINQYIR"
     gene            complement(4928..6202)
                     /locus_tag="PKX09_08780"
     CDS             complement(4928..6202)
                     /locus_tag="PKX09_08780"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOU17954.1"
                     /translation="MNKRLNILFLNWQPPFLLGIVIAYFAISVRFLPAAEKVDSEKAI
                     RKIADKVLNDANFNFIDQESGKHYFSTADAPPEARLVPESRYNDWRYWNGVLNIAMLR
                     LGEVLKEPTYTEFAVRNVAFSFDNYAYFEKRYANENKWEYPFGQFFMMEELDDCGAMG
                     ASVIEVMSYDPQERYLNYIDRAATHILTRQSRLEDGTLVRTFPRKYTLWADDLYMANS
                     FLCRMGKVSERSWQTIGIDDATRQVINFHKYLFDPKTGLMYHNWYSDTGKPGVAFWGR
                     ANGWAILAQVELLDQIQADYPQRDTLLALFRRHIDGIIPYQSQTGLWHQLLDKPDSFP
                     ETSCTAMFTYAIARAINQGYLESRYKSFALKGWQGVLTRIRPDGQVEGICTGTSTSDD
                     LNDYYTRPTPLNDVHGIGTVLMAGTEIIQLLK"
     gene            complement(6271..7308)
                     /locus_tag="PKX09_08785"
     CDS             complement(6271..7308)
                     /locus_tag="PKX09_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOU17955.1"
                     /translation="MTDQQWETLKKVINGEKMEPLPIGFIADSPWLPGWYGIKILDYF
                     SNDELWLKANLKAIQEFPEVMFLPGIWSEFGMCTEPSAFGARCRFPQNEFPHAHPVIK
                     SPEEINALVIPEPETDGLLPFVLNRLKLAQPYIENAGHKIRFAVARGPLNIASFLMGT
                     TEFLMAMLTSPELVETLMEKITAFLKNWLQLQRSTFSTIDGIFLLDDIIGFIGGQEFR
                     RFGLPYFKELFNADVAVKFLHNDAQCRVSVPFLPEIGVNLFNMGFDVSLNDIKTWTNN
                     QVTLLGNIPPRDVLANGKPEDVVKSVTELLDLLEDKTRVILSCGGGLPPGVGTENLKA
                     FIETVKNYTPK"
     gene            complement(7328..9010)
                     /locus_tag="PKX09_08790"
     CDS             complement(7328..9010)
                     /locus_tag="PKX09_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOU17956.1"
                     /translation="MKKVIIIMTLIVANFISAGAFSADQKAFYYNPILAGFYPDPSIC
                     RVGNDYYLVNSTFAYFPGIPVFHSRDLVNWRLIGHVMDRAEQMNLEGLGVSEGIFAPA
                     IRFHDGLFYVTCTLVGAGGNYVVTAQNPAGPWSNPVWLPQIDGIDPSLFFDQDGKAYI
                     VYNSVAPNNRPLYEGHRTIRMYEFDYKSLRVIGPEIILVNGGTDISKKPIWIEGPHIF
                     RKDNFYYLICAEGGTSWEHSEVVFRSEQVTGPYVPYAKNPILTQRHLDPNRPHPITCT
                     GHADLVMTPAGDWWAVFLGCRPYLPEYEGFFNTGRETFMAPVKWIDGWPVIDPDHNEI
                     QYSYPLPIILEKPIKADIPYSGNFRIKDDFDSEKLDLNWVFLRTPHTMWYSLSDRRGS
                     LAIRLRPETCSGKSNPSFIGRRQQHLRGSASTAMDFVPASEFEKAGLLIFQNEKHYYF
                     LGKSIEKGEPVVQLYKTDTLLASHKIGEGRNIRLKVVADGAEYSFYYATKPEEWILLK
                     DKVDARFLSTRVAGGFVGCMYAMYATSSGQASSNVAYFDWFEYVGDDEIYHQ"
     gene            complement(9045..>9969)
                     /locus_tag="PKX09_08795"
     CDS             complement(9045..>9969)
                     /locus_tag="PKX09_08795"
                     /inference="COORDINATES: protein motif:HMM:NF036499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOU17957.1"
                     /translation="MPEVIEINVPESAEMGVAIEGSTECWPPATSEAILPEFDPHNQS
                     CHYIEIFNRGKKPFKYEVKSGNPCVLISQKKGEITTEERLWIEIDWKQAPKGKSTIPL
                     IIKGPRKSQVEVKIIIFNPVVPIAELADRFLESNGYISIEAEHFSNAVNTRGIYWQII
                     PDLGRTLSSVTPFPVTMSSLIPEGDSPRLEYQVYLFNAGEVKVKAYLAPTQNFPNGPG
                     LRYGISFDDEPVQIVNMHAGKTHRDWQNSVSDYITIMTSAHQLKSPGKHTLKFWAVDP
                     GVVLQKLVIETGEVKPSYLGPPESRLFKIAE"
BASE COUNT         2791 a         2340 c         2004 g         2834 t
ORIGIN      
        1 aagcctttac cgtaagcact gggagtaaag ttatattcag caactatttg cataattcgc
       61 tgacgagtag cctcactcac atcctggcgg ccattcaagg ctttcgatac agtcgagagt
      121 gaacaatttg cgagacgcgc aatatcctta attgtcatcg ttaaatcttc ctaagtcaca
      181 ataattaaag taattaaaaa ccggtcatag taccgcacca atcctgattt ttgtatgcta
      241 ctagcggtca ttttccccta ccccgcgata gcctttatcc cttttcaatc catcaattct
      301 cttttacgaa atcgttttct gtatcctaat ttaaatgaca tttttaaaaa tgcaagaaaa
      361 attatttaaa ttttattttc tcgtcgagaa tatctcggtt ttagatccat tacaaattcg
      421 ttgaaaaatt ttacacctaa gcggtcacga aaatattttc gatagttcat cgaaaaattc
      481 cgaaatccat aaaaatcaat tgccttgttt caggaataat tggataatct attccgactt
      541 aattataaaa aatgttgcct tttaacaatc ttaaaagcaa catttacttg ctcgtatctt
      601 gagcaaatat attagtttat tggtacttat aaattttcta tcttaccgaa aaacgataga
      661 tggtagtctg gcgatattct tcgcccggtc gcagaactac cgatggaaaa tgcggtttat
      721 taggcgaatc cggaaaagcc tgcgcttcca gacagagagc acagcggtaa ttgtaacgaa
      781 taccgccttt tccgatcagt gaaccgtcaa gaaaattacc agaatagaat tgcaaaccgg
      841 cttgatcggt caggcattcc attaatctac cacttttaga ttcataaaca gttgcggcga
      901 gacgcacctg cccaggcata ccgttaatta cccagttatg atcataaccc ttgccgaatt
      961 ttatctgctc atcttcagcg gcgatgtccc gaccaatcgg tttgggtaca cgaaaatcca
     1021 gcggagtgcc gactaccggt cggatttcac cggtaggaat agaaaattca tcaatcggcg
     1081 taaagaaatc agcattcaac attaattcgt gatctaaaat agtatttttc ggcgatccgc
     1141 ttaaattgaa ataacagtga ctggtaggat tcagaatagt cggccggtcg gttgttccat
     1201 aatagtcaat tcgtaaagca tcgtcgtcgg taatggtata aacaacagat atttttactt
     1261 ccccgggata gcccttttca ccgtccggac tgacataagt caaccgaact gattggctat
     1321 ctagctgttc gatatcccat attactttga aaaaaccttt cggaccgccg tgtagatgat
     1381 ttggtccatc atttaaattg agctggtaga cttttccatc gagactaaat ttgccgccgg
     1441 ctattcggtt gccgtagcga cctacaatag cgccttggaa agtattgtcc ttttcgtaac
     1501 tggctaaatc atcaaatccg aggataatat cttccagctg gccgttcctg ttcggagtcg
     1561 tcaaagaaac tacgatagct ccatagctga taatttctgc cgaaactcct ttatcatttg
     1621 aaatcttgta ctttttcatt ttcgctccgt gttcctaatt tttcgattag cgataaactg
     1681 ccggcgttca actattcgtc tttgttttac tgagtttgat cagttccgct ctgagacgga
     1741 aaggatcatc acctttcaac ccgcgaatcc ggcaactgat agattcaaac atcgcgctta
     1801 gtttccgata aactggtaga ttggcttcga ttggctgttt gcgccttttt tcgtccagga
     1861 tcacaaatgg cttaaccaat tctttaagac ttacctcttt attctgggct ttactccagg
     1921 tccaagccgc atgcaaagcc gcacccagcg ccgcaccttc accttcgact ggtactgtct
     1981 gggcttcgaa aatatcagca atagtctggc accaggtatc agaccgcgaa agcccaccgg
     2041 tcaggcgaat ctctttcaca tccaccggca tgcgcttaaa accggtatag aggttaagaa
     2101 tatggccctc gataatagcg cgagctaaat tagccttggt aaaatcagcc aatccaaaac
     2161 cgaagtaaat tggcgcagcc aacggcaagt ccggagtccg ttcgcctaca taccagggaa
     2221 ttagtaattt accgttattg gctggcggcg tttcatcaag cattttatca aaaacctgat
     2281 gagtcatttt atgcagttta atgaattcgt tgtacccgtt agctaaattc gatacgcata
     2341 acaaaggtaa ataatggccg gtgctatcac aaaaagcggc aatttctccc gttggatcaa
     2401 tgtaaggctc ctccataaat gtataagccg ttccgcttgt cccaagactg acggtcacaa
     2461 tcccaggttc gacattccca gttccgactg caccatacat attgtctccg caaccggcat
     2521 caattaagca gtctttcgaa aaaccaaact tcgccactaa ttcgggagaa atatatccaa
     2581 tcgtttgatc ggaaggttta acttccggta atttggacat taaatcggga gcgatagctt
     2641 ggatgacttt ctgtgaccac tgaccatttt tggggttcca aagtgccatg ccggaggtgt
     2701 cacccggttc catcacgcgg acacctccgg tcagataata attgatataa ttatgcacca
     2761 gaaagagagt agccgtttta tggtaaattt ccggttcatg gcgcaccatg tgaaaaattt
     2821 tcgccgcagt atatccagta cgctggctat tgccaacttc ggcaatcatc tgttccagcc
     2881 caccgacttt ttctgtcaga atttcacatt cttccagagt cgaaaaatcg ttccagagtt
     2941 tacttgtggg tcgtgataaa ttgccctgcg catccagagc aaccagacca tgctgttggc
     3001 cggaaaccgc aatgcaaccg atctgagaca tatcaatccc agccgcgacg gcattagcaa
     3061 aaacgcgatc aaccgcttct atccacattc gggggtcaga ttcggagaca ccttctccca
     3121 gtccctggat agcgccgttc tttgtgttat agtgcggcag gtcggtatcg taattaaccg
     3181 aattaacata aacgactttc ttggccgcga gatcaataat gacaagtttg gtactttggg
     3241 tcgaaacatc caacccggcg aataacttaa acacggtttt actcctattt atgagattca
     3301 gcgaatatac tgattgatca ggctttcgta caattcttgt ttgccgctga tcattttggg
     3361 ttcgccaatt tcttcggcga ttttcgccag ttcgacgagg cctaattcac ctttctcgaa
     3421 acgagcaccg tcgccggaat caaaactact gtagcgttct tttttcatct tgagataatc
     3481 cgatttttcc agaatatcat tggcaatcag caacccgcgc gccagagtat ccatcgagta
     3541 aatatggcca atgaaaatat cttccaaatc ggtcgaacta cgacgattct tggcatcaaa
     3601 attcataccg ccgtggccga ttccaccatt ctgcaaaaga attagcatta gttcggtagc
     3661 atcatacaaa ttatgtaaaa attcatcagt atcccagccg ttaaaaggat ccccttggtt
     3721 aatatccaga cttccgaaca ggccggcatc gcaagccgtc tggacttcgt gcgcaaaagt
     3781 atgcccagct agggtagcgt gattattctc gatattgatc ttgaagtcat tctgaagacc
     3841 atatttgttc agaaaaccga ttaccgtagc cgaatcgaaa tcgtactgat gcttggttgg
     3901 ttccataggt ttaggttcga tcaaaaatgt gcctttgaaa ccagacgccc ggccataatc
     3961 gcgagcctta gtcagaaaca tcgccatatg ctcgatttcg cgtttcatat tagtattaag
     4021 caggctgtag taaccttcgc gaccgcccca gaatacatag ttttctccac cgagttcgat
     4081 agtcgcatca atggcggctt tcacctgtgc ggcgacgtga ggaataacgt aaaaatttgg
     4141 attagtggcg gcaccgttag tatagcgttt atgcccaaat acattagccg tgccccacaa
     4201 caatttgaca ccggactctt cttgtttggc tttggcaatt ttgaccagtt ctttgaggtt
     4261 tttttcagat tcgactacat tcttcccttc cggagccata tcacgatcgt ggaagcaata
     4321 aaacggtgta cctaacttag tgaaaaattc aaaagccgca tccagtttat ctttggcggc
     4381 ttgcattgga tctttagact cattccaggg aaaatcaact gtaccttccg ctccaaatgg
     4441 gtccccacca ttgttacaga atgtatgcca gtaagcaatg gcaaagcgaa aatggtcttt
     4501 cattttcttg ccggctacaa ctctgttctc atcataatac ttgaacgcca gaggattttt
     4561 tgattcagga ccttcataag gaattttacc gattcccttg aaatattccc gttgtccaat
     4621 taagacctgt tttgacataa ttaatctcct gttttttatt tactgttctt acgaatcata
     4681 gttttattaa gtgttaaatg atttgtaatt gaaaaaatca taatattcaa ttttgatagt
     4741 tttatttgcc tatattaata tttaaagttg ggtaggttaa aattttttta tagatatctt
     4801 tacatactac aatcataatc taaaatttac ttgaaccgtt tcaatattac caataaatat
     4861 tttaaaggtc ttattcagaa tggattttta tccgggacac accaagcgct aaatctatgg
     4921 tttgggttta cttaagcaat tgtattattt cagttccagc cattaagaca gttccaatac
     4981 cgtggacatc gttgagtggc gttggacgtg tgtaataatc gtttaggtcg tcgcttgttg
     5041 acgtaccggt acaaatccct tcgacctgtc cgtcaggacg aatcctggtt aagacgcctt
     5101 gccagccttt taaagcaaac gacttgtagc gggattccag atagccttga ttaatagccc
     5161 gcgcaatagc gtaagtaaac attgccgtac aggatgtttc cggaaatgaa tccggtttat
     5221 cgagcaattg atgccacaat ccggtctggc tctgataagg gataattccg tcaatatgac
     5281 gccgaaataa tgccagtaat gtgtctctct gaggataatc ggcctggatc tgatccaaaa
     5341 gctcaacctg agccaaaatc gcccaaccgt tggcgcgtcc ccagaaagca actcccggtt
     5401 ttccggtatc cgaataccag ttatgataca ttagcccggt tttcggatcg aacaggtatt
     5461 tatggaaatt gatgacctga cgagtggcgt cgtcaattcc gatagtctgc caggaacgtt
     5521 cagacacttt acccattcga cacagaaagg aattagccat atataaatca tccgcccaga
     5581 gagtatattt acgcggaaaa gttctcacga gcgtgccgtc ttccaaccga ctctggcggg
     5641 ttaaaatatg cgttgccgct cgatcaatat aattgagata ccgttcctgc ggatcgtaac
     5701 tcatcacctc gataacgcta gcccccatcg ccccgcaatc atcgagttcc tccatcataa
     5761 aaaactgacc gaaagggtat tcccatttat tttcattagc atatcgtttt tcaaaatagg
     5821 cataattgtc aaaactaaat gcgacatttc ggacagcaaa ttcggtataa gtcggctcct
     5881 tcagcacttc gcccagcctg agcatagcga tattcaaaac gccgttccag tagcgccaat
     5941 cattatagcg actttccgga accaaccggg cttcgggcgg agcatcggca gttgaaaaat
     6001 aatgtttacc gctttcttga tcaataaaat taaagttggc atcattcagg accttatcag
     6061 ctattttcct tatggctttt tctgaatcaa ccttttcagc agctggtaaa aagcggactg
     6121 atattgcaaa atatgcaatt acaattccca aaagaaaagg cggctgccaa tttaaaaaca
     6181 aaatattaag acgtttattc attaggactc atattttaaa attagatcga tttttcatcc
     6241 taacaaccca ggaaaatcaa gcgtgaaaat ttacttaggc gtataatttt tcaccgtctc
     6301 gataaaagct ttgaggtttt ccgtccccac acccggtggc aagccacctc cacaagataa
     6361 tatcacccga gttttgtctt caagtaaatc caacaactcg gttacgctct ttacaacatc
     6421 ttccggtttc ccgttcgcca gcacatcgcg cggcgggata ttgccgagta gcgtcacctg
     6481 attattagtc caggttttga tatcattcag gctgacgtca aatcccatat taaacaagtt
     6541 tacgcctatc tccggtaaaa atgggacaga gactcggcat tgagcatcat tgtgcagaaa
     6601 tttcaccgcc acatcggcat taaataactc cttaaaataa ggcaatccaa atcggcgaaa
     6661 ttcctgtccc ccgataaaac cgataatgtc atccagcaga aaaatgccgt ctatcgtgct
     6721 gaatgtactt cgctgaagct gaagccaatt tttcagaaag gcggttattt tttccattaa
     6781 tgtttcgact aactccgggc ttgtcagcat tgccattaaa aattcagtcg tgcccatcaa
     6841 aaaactggca atgttcaatg gcccacgtgc tacggcaaac cggattttgt gaccggcatt
     6901 ttcgatatac ggctgggcta atttgagacg attcaggacg aatggcaata aaccatcggt
     6961 ttccggttcg ggtataacca gcgcattaat ctcctcgggt gatttgatga ccggatgggc
     7021 atgtggaaat tcattttgcg ggaaccgaca acgcgctcca aaagccgatg gctcggtgca
     7081 cattccgaat tctgaccaaa tccccggcaa aaacataact tccggaaatt cttgaatagc
     7141 tttcagattg gctttcaacc acaattcgtc attggaaaaa taatcgagaa ttttgatccc
     7201 ataccagcca ggcaaccagg gactgtcagc aataaatccg ataggcaatg gttccatttt
     7261 ttcgccattg attacttttt tgagtgtttc ccactgttga tctgtcattt ttaatcctca
     7321 ctaagtatta ttgatggtaa atttcgtcgt cgccgacata ctcaaaccaa tcgaaatagg
     7381 caacattgga gctggcttga cctgatgatg ttgcatacat agcatacata cagccgacaa
     7441 atccgcccgc cactcgtgtg cttagaaagc gcgcatctac cttgtctttc agcagaatcc
     7501 attcttctgg cttggtggcg tagtaaaaag aatattcggc gccatcggcg acaaccttca
     7561 accggatatt acgcccctcg ccgattttgt gtgaagccag caacgtgtcg gttttatata
     7621 attgcacaac cggttcgcct ttctctatcg atttgcctaa aaaataatag tgcttttcgt
     7681 tctgaaaaat cagcaatcca gctttttcaa attccgaagc cggaacaaaa tccatcgccg
     7741 tactcgccga cccgcgtaaa tgttgctgac gccgtccgat aaaactcgga ttgcttttgc
     7801 cggagcaagt ttctggtctt agtcgaatag ccagcgaacc tctgcggtca cttagactat
     7861 accacatggt atgcggcgtc cggagaaaga cccagttcaa gtcaagcttt tccgaatcaa
     7921 aatcatcctt gattcggaaa ttaccgctat aaggaatatc agctttgatt ggtttttcca
     7981 atataatcgg cagaggatag gaatattgaa tctcgttgtg gtcaggatca ataactggcc
     8041 agccgtcaat ccattttact ggtgccataa aagtttctcg accagtattg aaaaaacctt
     8101 catactcggg aagataaggt cggcagccaa gaaatacagc ccaccaatcg ccagcgggcg
     8161 tcatcaccaa atcggcgtga ccggtacagg taatcggatg aggccgattc ggatcaagat
     8221 gacgttgagt tagaatcgga tttttcgcat aagggacata cggtccggta acttgctcgc
     8281 tccgaaagac tacctccgaa tgttcccagg atgtacctcc ttcggcacaa atcagataat
     8341 agaaattatc cttgcgaaaa atatgcggtc cctcaatcca gatcggcttt ttagaaatat
     8401 cggtaccacc attcaccaga ataatctcag ggccaattac tctaagggat ttgtaatcga
     8461 attcatacat tcggatcgtg cgatgccctt cgtataaagg tctattgttc ggcgcaacgc
     8521 tgttataaac gatatacgct ttgccatcct gatcgaaaaa tagcgaggga tcaatgccgt
     8581 caatttgtgg aagccaaacc ggattcgacc acggtcccgc cggattttga gcagtaacga
     8641 cgtaatttcc accggcacca accagagtac aggttacata aaacaaccca tcgtgaaatc
     8701 gaatcgccgg agcgaagatt ccttcggata cacccagccc ttccagattc atctgctccg
     8761 ctcgatccat tacatgaccg atcagtctcc aattgactaa gtcgcgactg tgaaaaactg
     8821 gtattcctgg gaaataggca aaggtggaat taaccagata atagtcattt ccaacccgac
     8881 aaatgctcgg atcgggataa aagccagcca gaattggatt atagtagaac gctttttgat
     8941 cagctgaaaa agcgccggcg cttataaaat tagcaacgat tagtgtcatt ataattatca
     9001 cttttttcat ttgattcctg cactttcaga attgaaaata aactttattc tgcaatttta
     9061 aacaagcggc tttccggcgg cccgaggtaa ctgggtttta cttcaccagt ctcaattacc
     9121 aatttttgga gtaccacgcc gggatcgacc gcccagaatt ttaaggtatg tttgccgggc
     9181 gatttcaact ggtgggcgga agtcattatc gtaatatagt cactgacgga attttgccag
     9241 tcacggtggg ttttgccggc atgcatattg acgatttgaa ccggctcatc atcaaaagaa
     9301 atcccatagc gtaagcccgg accgttggga aaattctggg ttggagccag ataagcctta
     9361 actttcactt cgccagcatt aaataaataa acttggtact caagacgtgg gctatcgcct
     9421 tccggtatca gactggacat cgtcaccgga aatggagtaa ctgaggaaag cgtacgtccc
     9481 agatcgggaa taatttgcca gtaaatacca cgcgtgttga cggcattgga gaaatgctca
     9541 gcttctattg aaatataccc attgctctca agaaaacggt cggcaagctc cgctatagga
     9601 acgaccggat taaagataat tattttcacc tccacctggc ttttccgggg acctttgata
     9661 ataagcggaa ttgtgctctt ccctttgggc gcttgcttcc aatcaatttc aatccagagc
     9721 cgttcttcgg tagtaatttc tccttttttc tggctaatta gaacgcaggg attgccggat
     9781 ttaacttcgt atttaaaagg ctttttaccc cgattaaaaa tttcgatata atgacaggat
     9841 tgattgtggg gatcgaattc tggtaaaatc gcctcgcttg tcgcaggcgg ccaacattcg
     9901 gttgaacctt caatggcaac gcccatttcg gctgattccg gtacattgat ctcgattacc
     9961 tctggcatt
//
LOCUS       DAOSMP010000064         8867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_7857_length_8867_cov_14.346459, whole genome shotgun sequence.
ACCESSION   DAOSMP010000064 DAOSMP010000000
VERSION     DAOSMP010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8867
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7857_length_8867_cov_14.346459"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PKX09_08800"
     CDS             <1..534
                     /locus_tag="PKX09_08800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOU17958.1"
                     /translation="GEDTKIWHFCHIQSGAKIGRGCSIGQNVNVGNNVVIGNFVKIQN
                     NVSVYEGVTLEDYVFCGPSMVFTNVIDPRCKYPQRGAEFYRKTLVKEGASIGANATIV
                     CGNTIGRHAFIGAGAVVTKDVPDYALMVGVPARQIGWACECGEILPKFEKEIKCPRCG
                     LEYLLENGVLLAKDAKE"
     gene            561..938
                     /locus_tag="PKX09_08805"
     CDS             561..938
                     /locus_tag="PKX09_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOU17959.1"
                     /translation="MNENEVAKNFLDTAFEIHTKFGPGLFESVYEKLLAYQLPKKGFP
                     VERQKILPLLYENLKIESGFRIDLLVDDAVIVEFKSIESVLPVHKKQFLTYLRLSGRH
                     LGLLINFNEAHLKDGITRIVNNL"
     gene            1138..2166
                     /locus_tag="PKX09_08810"
     CDS             1138..2166
                     /locus_tag="PKX09_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU17960.1"
                     /translation="MYNIALIGCGRISKNHFESINQLSNDLKLVAVCDIIEERARSAG
                     EKYGVDWYTDYDKMLERDDIDIISICTPSGLHPEHGIKAAQKGIHVISEKPMAISLAG
                     ADALIKACDDNKVQLFVVKQNRLNTTSQLLKRAVDKGRFGKIYMAQTNVFWQRPQEYY
                     SQAKWRGTWEFDGGSFMNQASHYVDALLWLLGPADHVMAETATMARKIETEDTGSAII
                     KFRNGAIGTINVTMLTYPKNFEGSITILGEKGTAKLGGVAINKIEKWEFEDYDDDDRL
                     VHEANYNPPNIYGLGHLPYYKNVIAVLNGAEEANTDGRSGRKSLELIIAIYKSAQTGQ
                     KVALPLKL"
     gene            2467..3762
                     /gene="tviB"
                     /locus_tag="PKX09_08815"
     CDS             2467..3762
                     /gene="tviB"
                     /locus_tag="PKX09_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HOU17961.1"
                     /translation="MSTDRISIIGLGYVGLPLAVEFAKNFPVTGFDIKKERVEELRNG
                     HDATLEVEDDHLKSVLINQNGRTGLFLTDQLDDIRPCNYYIVTVPTPIDQYKRPDLTP
                     VIKATETVSKVLKKGDIVIYESTVYPGCTEEVCVPILEKGSGLHFNTDFFCGFSPERI
                     NPGDKIHTVTKIKKVTSGSTPEIAHKIDALYQTIITAGTFLASSIKVAEAAKVIENTQ
                     RDLNIAFVNELAKLFNMMSIDTKEVLEAAGTKWNFLPFQPGLVGGHCIGVDPYYLTFK
                     AQGIGYHPEVILAGRRINDSMGFYVAEQVVKLMIKKGHTINASQVLILGITFKENCPD
                     VRNSRVIDIITELQQYGCQVDVYDPWADSKEVKREYGIDLVAQFSQKMGNKQLPHYDA
                     IVLAVAHNEFKTLNLKPLKNNRTVIYDVKGILAEYDGRL"
     gene            4280..4715
                     /locus_tag="PKX09_08820"
                     /pseudo
     CDS             4280..4715
                     /locus_tag="PKX09_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682674.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
     gene            4821..5429
                     /locus_tag="PKX09_08825"
     CDS             4821..5429
                     /locus_tag="PKX09_08825"
                     /inference="COORDINATES: protein motif:HMM:NF027589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HOU17962.1"
                     /translation="MSKYLVITADMINSRDVSTEQIATIPEKLKQIDADFHTLIPSML
                     FAGDEIQLLFPYDQNHHPYRLLMDLNRHLAPFHFRFGFGIGAIDQPINPIIAHNKGQA
                     FLQARKALAIAKKDQLIAWLSTEEIVQYSEINACFLLLTALTRKWSDKHWRRFYLYWD
                     NHSIHKVAEIENVSPESINKLLNNAGIRLIIRSLELITELHE"
     gene            5516..5875
                     /locus_tag="PKX09_08830"
     CDS             5516..5875
                     /locus_tag="PKX09_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17963.1"
                     /translation="MLTKVLAYTSLYIVTIFVNGIIVKLLLKPFLPEDMPAGTPNAGR
                     MIGYLERALILTFLIIEQPTLIGFVLTIKAIFRYGDIQGDCNTKMKISEYFIIGTLIS
                     VLLTVLVYAIFRNAHLL"
     gene            6031..6324
                     /locus_tag="PKX09_08835"
     CDS             6031..6324
                     /locus_tag="PKX09_08835"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOU17964.1"
                     /translation="MNKSEILNELKRYLDNSRYKDRIRKIGVFGSVARGEISAGSDID
                     IFVDLSPVKAFDLIGIKQDIEKLTEKHVDIVIIRDNMNPFLKSRIEREGIIVR"
     gene            6314..6688
                     /locus_tag="PKX09_08840"
     CDS             6314..6688
                     /locus_tag="PKX09_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOU17965.1"
                     /translation="MYDKSLSIEILQQTLTACKRVTNRFKGIVTAEDFLKDERGLEKL
                     DAICMQLIAIGESLKNLDKITNMELLVLYPAIEWQKAMGLRDIISHHYFDINAEVIYD
                     VCQNEIPKLSLEISKIIEDLGN"
     gene            7156..8427
                     /locus_tag="PKX09_08845"
     CDS             7156..8427
                     /locus_tag="PKX09_08845"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU17966.1"
                     /translation="MLSKDQIKQILADQRATILNKSLGIERTLLSDIDKTIKLPQVVV
                     ITGLRRVGKSTLMRQIIKKYYNDQHFYYINFEDERLFKFKAEDFNLLYEALIELYGEQ
                     RTFFIDEIQNIEHFENFVRRFYDSGFKFFITGSSARLLSREIGTKLTGRHIDMVVRPF
                     SFCEYLSLKEVSLEEEMLYNTVERTKIKSHFQNYLVSGGMPEYLLYNEPEILSRIYED
                     IVIKDIAVRYNVANLSEMRDIYLYLITNVANKFSFNKLKTISGVGSITTIKNYLHYLS
                     ETYFLQVINKFDYSLKKQMVNDKKAYIIDNGFFNVLSTKMTQDTGWLLENLVCNVLSA
                     NYRLYYFSDKYECDFVVQKENRIVSAIQVTAEINNINEKREYNGLLNALKTFNLKEGL
                     ILTNDQESTIQIEDKTIHIKPVWKWLVEAER"
     gene            8461..8805
                     /locus_tag="PKX09_08850"
     CDS             8461..8805
                     /locus_tag="PKX09_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015155898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOU17967.1"
                     /translation="MKFERFEEIIAWQKAKEMTTQIYTLFKDNKDYGFKDQIQRSSVS
                     IMNNIAEGFERRSNIEFRQFLFIAKGSCGEVRSMLYLARSLDYLSESDFINLLNNSVE
                     ISKTLSGLIKTL"
BASE COUNT         2928 a         1625 c         1706 g         2608 t
ORIGIN      
        1 ggcgaagata ccaaaatctg gcacttctgt catattcagt ccggtgcgaa aatcggtcgc
       61 ggctgttcga ttgggcagaa tgtcaatgtc ggcaacaacg ttgtaatcgg caattttgtg
      121 aaaattcaga ataatgtctc ggtctatgaa ggtgttactc tcgaagatta tgtcttttgt
      181 ggaccttcga tggtttttac gaacgtaata gatccgcgtt gtaaatatcc ccaaagaggc
      241 gcggaatttt atcgtaaaac tctggttaag gaaggcgctt cgatcggcgc taatgctacg
      301 attgtttgtg gaaatacaat cggtcgtcat gcttttattg gagccggagc agtggtgacg
      361 aaagatgttc ccgattatgc tttaatggtc ggagtacctg cccgtcagat tggctgggct
      421 tgcgaatgcg gcgagattct accaaaattt gaaaaagaaa tcaagtgtcc acgctgtggg
      481 ttggaatact tgcttgagaa tggggtactt ctcgccaagg atgcaaagga ataaagagtt
      541 cgcaaaagtg ttcttaagta atgaatgaga atgaagttgc aaaaaatttt ctcgatacgg
      601 cttttgaaat tcacaccaaa tttggaccag gtttatttga gtcagtttat gaaaaattac
      661 ttgcttacca gctaccgaaa aaaggttttc cggttgagcg gcaaaaaata cttcccctac
      721 tttatgaaaa tcttaagatc gagtcaggtt ttaggatcga tttattagtt gatgatgctg
      781 taatagttga gtttaaatca atagaatcag tcttaccagt tcataagaag cagttcttaa
      841 cttatttgcg attgtcaggg agacatcttg gtttgttaat caattttaat gaagcccatc
      901 taaaagatgg tattactcgc atagttaata atttataaaa ctatataaaa atactttgtg
      961 tccgtttgaa actttgcatc cttggtgata aatagactat aaaggtttaa taaaatactt
     1021 tgcgttcttt gcggttaaac aaggatgaaa ccgccaagga cgccaagtga cgctaagaga
     1081 agaatgtaat aaataaaaaa ccttagcgtt ctttgcgaga aaataaacga ggtaatgatg
     1141 tacaatattg cccttattgg ttgcggtcga atttctaaaa atcactttga atccatcaat
     1201 caattatcta atgacctgaa attagtcgcg gtctgcgata ttattgagga acgtgcccgc
     1261 tctgccggtg aaaaatacgg agttgattgg tacactgatt atgataaaat gctcgaaaga
     1321 gatgatattg atattatttc aatctgtacg cccagcggtc tgcatccgga acacggcatt
     1381 aaagccgctc agaaaggcat tcatgtcatt tccgaaaaac cgatggctat ttcactggcg
     1441 ggcgctgatg ccctgattaa agcctgcgac gataacaaag ttcagttatt tgtcgtcaaa
     1501 caaaatcggc taaatactac ttcccaacta cttaaaagag cagtggacaa aggccgtttt
     1561 ggaaaaattt atatggcgca gacgaatgtt ttctggcaaa gaccgcagga atattattcg
     1621 caggcaaaat ggcgcggcac ttgggaattc gacggtggtt catttatgaa tcaagccagt
     1681 cattatgttg atgcgttact ctggctgtta ggcccagcgg atcacgttat ggctgaaacc
     1741 gctactatgg cgcgtaaaat cgagaccgaa gacaccggct cggctataat taaattccgc
     1801 aatggcgcaa ttggaacgat caacgtcacg atgctgactt atcctaagaa tttcgaaggt
     1861 tctataacaa ttctcggtga aaaaggcacc gctaaacttg gcggcgtagc catcaacaaa
     1921 atcgagaaat gggaatttga ggattatgac gacgacgatc gtttggttca cgaagccaat
     1981 tacaatccac caaatattta cggcttagga catttgcctt attataaaaa tgtgattgcc
     2041 gttttaaatg gagccgagga agccaatacc gatggacgca gtggtcgcaa gagcctcgaa
     2101 ctgattatcg cgatttataa atcagcccaa accggtcaga aagttgccct gccgctgaag
     2161 ctataagagg ttaaggtcga ggtagaggta gagggaagga aaaaacaaag gtgagtagag
     2221 aaggtgttta aaagttgcaa agatatgccg aactggcagg aggctatcga gatcgcagaa
     2281 tatggtcaca aaataaaata tttcaatgat gatatttagg atgatctcaa taaaatcatc
     2341 atttttttaa acgattcaca agaatctatt acaattagac cttatcgaaa ttctggttca
     2401 aatcccaatg tctaatttta cacaaaaact ttgccttaaa cttaacctaa ataaatcgga
     2461 gaatctatga gtaccgacag aatcagcatc attggacttg gttacgtcgg attgccgctg
     2521 gcagtcgagt ttgctaaaaa ttttcccgta accggattcg atattaagaa agaacgagtg
     2581 gaagaattac gaaatggcca cgatgccacc ctggaagttg aagacgacca cctgaagtcg
     2641 gtcttaatca accaaaacgg gcgaactggt ctgttcttaa ccgatcaact cgacgatatt
     2701 cgaccctgca actattacat tgtcaccgtg cctactccta ttgatcaata caagcgtccc
     2761 gatctgacgc cggtcatcaa agccaccgaa acggtcagta aagttctcaa aaagggtgat
     2821 attgtcattt atgaatccac ggtttatccg gggtgcactg aagaggtttg tgtgcccatt
     2881 ctcgagaaag gcagtggctt gcatttcaat accgactttt tctgcggctt ttctccagaa
     2941 cgaatcaatc ccggcgataa aattcatacc gtaacgaaaa ttaaaaaagt tacttccggt
     3001 tccacacccg aaattgctca caaaattgat gctctttacc aaactatcat cactgccgga
     3061 actttcctgg cttcatctat aaaagttgcc gaagccgcta aagtaattga aaatactcag
     3121 cgcgatctca acattgcgtt tgtcaacgaa ctcgccaagc ttttcaatat gatgagcatt
     3181 gatactaaag aagtattgga agcggctggg actaaatgga attttctgcc tttccaaccc
     3241 ggtttggttg gcgggcattg catcggcgtt gatccatatt atttaacatt taaagctcag
     3301 ggcataggct atcatccgga agtcatttta gcgggacgaa gaatcaacga ttcaatgggt
     3361 ttttatgtgg ctgaacaagt cgtcaaactg atgatcaaaa aagggcacac aattaatgcc
     3421 tcgcaggtat tgattctggg catcaccttt aaggaaaatt gccccgatgt gcgcaattct
     3481 cgagtcattg acattattac tgaacttcaa caatatgggt gtcaggttga tgtttacgat
     3541 ccttgggcgg attccaagga agtcaaacgt gaatacggaa tagatttagt cgctcaattc
     3601 agccaaaaaa tgggtaataa gcaacttccc cattatgatg ctatcgtatt ggcagttgct
     3661 cacaatgaat ttaaaacatt gaacttaaag ccattgaaaa ataatcgaac cgttatctat
     3721 gatgtcaaag gaattctggc ggaatacgac ggaaggttat agcaagatac tttttctgag
     3781 ataatcgcaa gagtaggtaa aaagtgattt agcgcacatc aatctcaact aaacattggt
     3841 tcgatcttaa aggattaaaa tttgattgac atcctaacta tgatttatta atttaaatta
     3901 tcaattagct tgtcacagga aatgggaaag aattattaaa gtatgtcact caaaatgcct
     3961 gaaacgaatg ccctgaaaag ctgttcctga caataactca ccgatgattt gaggcggctt
     4021 aatttagccg tgagaaacca gaatataact atgctccgtc gaagaatgta gttgacgggg
     4081 ctgtgccata cccggaaaaa ttgtaaaccg ccaagtacgc caagagtcgc caagaaaaat
     4141 aaacagggac ttaatgatat tagtggaatt ataaataaaa atactttgca gcctttgcgt
     4201 ctttgcgaga gaaaagagat atttctcgcc acgttcgcaa agtaaaaata atttagaatt
     4261 tagaatgtag aattagtaaa tggataaaga taacgcagtc aaagataaaa gttttcaatt
     4321 tgcacttgaa atcatttctc tttataaata tttgacgact cagaaaaatg aatttatact
     4381 atcgaaacaa atactaaggt cggggacttc tattggggca aacatcgaag aggcattatc
     4441 gtcgcaaagt aagaaggaat tcatcgcaaa acttcaccct gttaaatttt cttttattac
     4501 ttgaataaat ttataaaaat ggagaatgtt attgaaaatt atttagcagg gtaaatattt
     4561 cattgaaaga aaccaaagag acaaactatt ggttaagatt attaaaatca tccggatata
     4621 ttcaaaagga tgaattaatc gagaagacag aagaactatc aaaaatactg acatctatca
     4681 tcatttccac caaacagaat tcggagaaaa aataattctt ccgaaggatt gcctttggca
     4741 taaattctaa attctaaatt ctacattgat acaagcgttc ttagcgttct tagcggttaa
     4801 taaactatga ttaattttct atgagtaagt atcttgtcat tactgcggat atgatcaata
     4861 gtcgagatgt ttctacggaa caaattgcga caattccgga aaaattaaag caaatagacg
     4921 ctgattttca caccctgatt ccttcaatgt tatttgctgg cgatgaaatt caattgcttt
     4981 ttccttatga tcaaaaccat cacccctatc gcttactaat ggatttaaat cgccatctgg
     5041 ctccttttca ttttcgattc ggttttggaa taggcgctat tgatcaacca atcaatccaa
     5101 ttatcgctca taacaaagga caagcattct tacaagcccg aaaagcactt gccattgcca
     5161 aaaaagacca gcttatcgcc tggctatcca ctgaagaaat tgtccaatat tccgaaataa
     5221 acgcctgctt tttactcttg accgccttga ccaggaaatg gtctgataaa cattggcgtc
     5281 ggttctatct atattgggat aatcacagca ttcacaaagt cgccgaaatc gaaaatgtat
     5341 ctcctgaatc cattaataaa ctgctgaaca atgccggtat ccgattaatt attcggagtc
     5401 tggaactaat tactgagttg catgaataaa tgtcaactat attcgttgat aaataaaatt
     5461 gtcaacctct attgccaatt caagaacgat aaaatgctaa taatcaaaga ttaaaatgct
     5521 gacgaaggtt ctcgcttaca cttccttata tatcgtaact atttttgtaa atggaattat
     5581 cgtcaaatta ttacttaaac catttctacc tgaagatatg ccggccggaa cgccaaatgc
     5641 cggtcgcatg atcggttatc tcgaacgggc tttgatcctg acctttttga ttattgagca
     5701 accaacgttg ataggatttg ttcttacaat taaagccatt ttccgttatg gtgatattca
     5761 gggcgattgc aatacaaaga tgaaaatttc tgaatatttt atcataggaa ccctcatcag
     5821 cgtattattg actgttctcg tctatgctat ctttagaaat gctcatttac tttaaaacaa
     5881 aaacccctct gcgtttcttt gcctgcccat tagggcgttc tctgtcccgt ccttacggga
     5941 ctccttccgg agcggttaaa taaagatgaa accgctaagt tcgccaagtg tcgccaagcg
     6001 caagaaataa taaacaaaag ttggatatac atgaataaga gcgaaattct taatgaatta
     6061 aagagatatt tggataactc tcgatataaa gatcgcatta gaaaaatcgg tgtattcggt
     6121 tcagttgcaa gaggagaaat ttcagctgga agtgatatag acatttttgt tgacctatca
     6181 ccagtaaaag cctttgatct gattggtatc aaacaagaca ttgaaaaatt aactgaaaag
     6241 catgttgata tagttataat cagagataat atgaatcctt tcttaaagtc gagaatcgaa
     6301 agagagggga taattgtacg ataagtcatt atctatagaa atcttacagc agactttaac
     6361 agcttgcaaa agagttacta acagattcaa aggaatcgtt actgctgagg attttttaaa
     6421 agatgagaga ggtcttgaga aattagatgc tatctgtatg caattaattg caatcggaga
     6481 aagtctgaaa aatttagata aaattactaa tatggaattg ttagtactgt atcctgctat
     6541 tgaatggcaa aaggcaatgg gacttcgaga tataatttca caccattatt tcgatatcaa
     6601 cgctgaagtc atctacgatg tatgccagaa tgaaattcct aaattgtctc ttgaaataag
     6661 taaaataata gaagatttgg gaaattagtt tttgaaattc tatctctatt aaaaacaaat
     6721 atgattatct attaaatagt aaatttcata aaatttcctc ttatcacaat taatttatca
     6781 ggaacttaag aattgtaaag attatggact tcgtgatcaa aaatgctatt tggtttgata
     6841 aagaaacgac aagacaagtt ttgattcctt tctcctgcct cctttctcct gtctcctgcc
     6901 tcctgtcttt aatattcagt ccttcgcggt taaataaaga taaaaccgcc aagtgcgcca
     6961 agttcgcaaa gtaacaataa taattaataa aactttgcgt tctttgcgtc cttcgcggtt
     7021 aaagaaaaac ctaacccgcc aagcacgtca gcagtcgccg ataaccaaaa tttcgaatta
     7081 ctcaatctct ataatatctt atgacagtga acaatatctt attaaattgt tcaatgttaa
     7141 tgaagaatat taataatgct cagtaaagac caaatcaaac agattctagc tgaccaacgc
     7201 gcaactattc tcaataaatc tctgggaata gaacgtaccc tgctatccga tattgataaa
     7261 acaatcaaac ttcctcaggt tgtcgtcatt accggcctgc gccgcgttgg taagtcaacg
     7321 ctaatgcgcc agattattaa aaagtactac aacgatcagc atttttacta tatcaatttt
     7381 gaagatgaac gcttgtttaa gtttaaagct gaagatttca atcttctcta tgaagccctc
     7441 attgaattgt atggcgaaca gcggaccttc ttcattgacg aaattcaaaa cattgaacac
     7501 tttgaaaatt ttgttcgccg tttttacgac tccggcttta agtttttcat taccggctca
     7561 agcgctcgtc ttcttagccg cgaaatcggc accaaactaa ccgggcggca tattgatatg
     7621 gttgtacgtc ccttctcgtt ctgcgaatat ctatctctaa aagaggtttc tttagaggaa
     7681 gagatgctgt ataacactgt cgaacggact aaaattaaat cccattttca gaattatctt
     7741 gttagtggcg gtatgccgga atatcttctc tataacgaac ccgagattct cagccgcatt
     7801 tatgaagata tcgtgataaa agatattgct gtccgttata atgttgccaa tctatctgaa
     7861 atgcgcgata tttatcttta cctcatcacc aatgtcgcta ataaattcag tttcaataaa
     7921 ctcaaaacga tcagtggcgt gggcagtata accacaatta aaaattatct tcattatcta
     7981 tctgaaacct attttcttca ggtaattaat aagtttgatt atagtcttaa aaagcaaatg
     8041 gtaaatgata aaaaggctta catcatagac aatggtttct tcaacgttct ttccacaaaa
     8101 atgacacaag ataccggttg gttattggaa aatctggtgt gtaatgttct gagtgctaac
     8161 tatcggctgt actactttag tgataaatat gaatgcgatt tcgtcgttca aaaagagaat
     8221 agaatcgtct ccgctatcca ggttactgct gaaataaaca atataaatga aaaacgcgaa
     8281 tataacggat tattgaatgc tttgaaaacc tttaatctca aagagggact cattcttacc
     8341 aatgatcagg aatcaacaat tcaaatagag gataaaacta tccatatcaa accagtctgg
     8401 aaatggctgg tggaagccga gagatgaaag ttaaaagtta gaaagtttat aaagttaaaa
     8461 gtgaagtttg agcgattcga agaaataatt gcgtggcaaa aagcaaagga gatgacgact
     8521 caaatatata ctttattcaa agataataaa gattacggat ttaaggatca gatacagaga
     8581 tcatctgttt ctattatgaa taatattgct gaaggctttg aacgaagaag taacatcgag
     8641 tttagacaat ttcttttcat tgctaaagga tcatgcggag aagttcggtc tatgctttat
     8701 ttagcgagga gcttagacta tttatcagaa tcagacttta taaacctttt aaataattct
     8761 gttgaaatct caaaaacatt gtccggtctc ataaaaactt tatgaacttt taacttgata
     8821 aactttttac tttataaact ttcaactttc taaactttca actgctt
//
LOCUS       DAOSMP010000065         8589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_8179_length_8589_cov_22.088587, whole genome shotgun sequence.
ACCESSION   DAOSMP010000065 DAOSMP010000000
VERSION     DAOSMP010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8179_length_8589_cov_22.088587"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="PKX09_08855"
     CDS             <1..1062
                     /locus_tag="PKX09_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOU17968.1"
                     /translation="GITVFFVNNAIYGMTGGQMAPTTLIGQKTMTTPYGRNANNEGYP
                     IGMSEIIATLKAPVYVERVMIGDTKSILHARQAVRKALKLQIEKNAFCFIEVLSPCPS
                     NLKMSAIRSREWVCEVLQKQFPLGVKKDISNEFQSRDLKRPTIDRSQLHSILNIEPIT
                     VDKEKIAPYKKTNFSAEIKVAGFGGQGILSLGLVLAEMGMRHEYEVSWLPSYGPEIRG
                     GTANCSVKISNRKIGSPVVANPTILIAMNRPSLDKFENDVISDGLIIYDDSIIDRAPL
                     RQDVRVLPIPATKLADELGNSKAANMIIVGALIEYLGFMSVEVVLNSLGEVLRQKNLV
                     ELNQKALLKGVDFTRKIKS"
     gene            1271..4123
                     /locus_tag="PKX09_08860"
     CDS             1271..4123
                     /locus_tag="PKX09_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17969.1"
                     /translation="MKFIFTTDRIFLRILLIAILSVFILVNSLLAQMLGSDQPPPDIH
                     WRQIETQHYRIIFPAEIESESQRMANTLEYVYLPLRKTLKANSPKWPLVLSNRSAVAN
                     GYVTLAPRKSEWFTTPDQSAMSGNIEWYNLLATHEGRHMVQFDRFNSGFTRLAGFLFG
                     EYAILGLSFWHAPMWFWEGDAVAMETALTESGRGSQPEFDLPIRTLLINQKLYSYDKA
                     YLGSYKDYYPNYYHLGFLMVTHGRRQYGANIWDHVLTSSTRRSWNPFGFSKACRINTG
                     RKTKELYYDTMTELDSLWCKPIEGLEITSAKKVNLKPKKVWTQYRWPQYATDGSIIAV
                     INGLGDINTLVKINPDGQETEIAKIASANRFSTAGNLIAWEELTPHPRWGAAIFSDIV
                     VYDLLVGQERKLTKNGKYFSPAVAPHGQMIAAVKFTPERKCNLVLLNSNDGQEIRTLP
                     NPDNGFIKNPAWSPDGRQLVYFKQSGSSKSLAIYDLENNTEEIIIAECHSFDEFPTFY
                     GDYLLYNSAWSGIDNIYAVEIDSGRRFQVTSRKYGAYYPAVSADGSMMLFSDYDFNGL
                     DVCAMHLEPARWQALEAVERCDIRYYETLVAQEQGGNIFEAGKIPTRSYPVSDYDPYR
                     HLFNFHSWYYFFSETSANLGLFSNDILNNAAANFELNFDANEKAFSVGSEVCYAGFYP
                     ILSAGFNLGKRISYSGGLFDELTTETWNETSFSLGANLPLDYSSSVFNRKLNLGLSGA
                     YTYISDKTTVEDYEIGNGGFVPFSYEIAYQNYQTPAIRDVKPTAGYTVKANFKHTPLG
                     GDYQSQMYSFQGNYYLPGLAKHHSLMLNAKVELQNPDNYRFASLVPFAKGYDYIYNDR
                     LAGAGATYELPLAYPDWALGSLFYLKRLRGAFFGDFNVGKTSDFKQQYNSVGLDLNFD
                     FNLLQLAVELNAGVRLAYRLAEKDLHASLLIFNIEF"
     gene            4190..6406
                     /locus_tag="PKX09_08865"
     CDS             4190..6406
                     /locus_tag="PKX09_08865"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOU17970.1"
                     /translation="MKPNDNINQVMIKIGGMHCAMCVKTVADALSKVPGVRAVNVNLA
                     NEKAYLEFDNGHFSIEAAKRAIESVGYQYLGREGEQDLIEQDKTLNRELRSRKRRIII
                     GFITGVILMIPMFIKISLPFPIAYLMLALATPVFIYLSYPIFRAAGQALRNHSLNMDV
                     MYVMGIGVAFLASLLGTFEIVLTREFMFFETAIFLATFLTLGRYLETRAKGKTTAAIK
                     RLIGLQPQTVIVIRDNVESEIPLESVVINDIVVVKPGSRVPVDGVVISGESYIDESML
                     TGESLPVFKQTGAKVVGGTLNQNGILHIRAERIGRDTVLAQIIRLVEQAQGSRPPVQR
                     LADVAVSYFIPVILAIAILSFGFWYFVAEQTLLFALTAFISVLVIACPCALGLATPTA
                     VTVGIGRGAELGILIKNGTVLEVAPRVTTVVFDKTGTLTTGKPLVTDIFPVGISTAEL
                     LRYAAAVEKNSEHPLSTAIVNYAKELGIAIPESAKFAAYGGRGVTAYVDNRAVFSGTE
                     LFFQENKITISPDILAPAEKYTDEGKSIVFVAIDGQAVGVIAIADTLKDTAASAIEKL
                     RKDGLKIAMITGDNRRTAQAIAAQVGIETVMAEVLPQDKATVIRQMQTRGEVVAFVGD
                     GINDAVALAQANIGIAVGSGSDVALESGDIVLVKNDLNDVSAALQLSRKVMSRIRQNL
                     FWAFAYNAALVPVAAGALYPLFGITFRPELGGLAMALSSVTVISLSLNLRRFNPAR"
     gene            6469..7461
                     /locus_tag="PKX09_08870"
     CDS             6469..7461
                     /locus_tag="PKX09_08870"
                     /inference="COORDINATES: protein motif:HMM:NF017832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HOU17971.1"
                     /translation="MDLTQIKTIAAGFYIVDENGKKLPGTIKIPYAEKDGFIRHISQV
                     EKGLACGCSCPVCHQPVIARKGAHKRHHFAHYPDAVCNAETVLHYLAKRLLFEKLQTL
                     LKRKVALVLRWKCQLCGDQHSFNFFENVQAIVSEKTLSDCRPDLCLVDQHGAPTALIE
                     IVVAHPPDEKVLIFVSRKQIPLLIFRIRSGEEMEEMATCPTINPDLIIHCPRRRCPEC
                     GAPLFEKSLYILPAVCWRCGAGMNLAFLDIDGQVAGTEEFSAKDRQIISRNNVILRDT
                     IRPGDRKRTVSHICPHCGVVTGNAYLRYLKRHEQRLLVQNSGKYCRQCHRHFDE"
     gene            complement(7529..>8589)
                     /locus_tag="PKX09_08875"
     CDS             complement(7529..>8589)
                     /locus_tag="PKX09_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOU17972.1"
                     /translation="LDYFNNISYTTEPAGEGKINLILNVVEHTPQKLRLGVRYDDFHY
                     LVAALNFQTTSNIVHGLRLDVEWQFIGLNQIKLKTLYPSRRFQLPFYPFVNAHYREIP
                     TYVYSINGNRIASYIDRSALFGGGFGLLYKNYWNFEAELDYESINIKPDIAPDEPLRF
                     YDWNDDLYKFQLSSNIDLLDNAFMPRKGALVHCVYEKTISQIGRHTNYRRFELSADVY
                     QSIKRNTLHFSGFYGNAKMDGSTNRFIYRGGPETFVGVEYDQLVGSEMTILRLDYAYN
                     LLHNLQAKAIVNTAIGFRNIYHETIKESQNLLGYGIGLKYVSPLGPFDLVLGWGDKSV
                     FQPGEIRAVIYFRAGFLF"
BASE COUNT         2303 a         1913 c         1971 g         2402 t
ORIGIN      
        1 ggcataacgg tttttttcgt caataatgcg atttacggca tgaccggagg gcaaatggcg
       61 ccaacgacat taatcgggca aaaaactatg acgacgcctt atggaagaaa tgctaataac
      121 gagggctatc cgattggtat gtcggaaata atcgctactc tcaaagcccc tgtttacgtc
      181 gagcgcgtta tgattggtga caccaaatca attctccatg cccgtcaggc ggttcgtaaa
      241 gccctcaaac ttcagatcga gaagaatgcc ttttgcttta tcgaggtgct ttcaccctgt
      301 ccctcaaacc tcaagatgtc agccatccgt agtcgcgaat gggtatgcga agtacttcaa
      361 aaacaatttc cactcggagt gaaaaaagac atttccaatg aatttcagag tagagattta
      421 aaacgtccaa caatcgatcg ttcccagctt cattctatat taaatattga accgatcacc
      481 gtagataaag aaaagatcgc accctataaa aagactaatt tttcagcaga aatcaaagtc
      541 gccggctttg gcggtcaggg aattttaagt ctcgggcttg tcctagctga gatgggtatg
      601 cgtcatgaat acgaagtcag ctggctacca tcttatggtc cggaaatacg tggtggcacc
      661 gccaattgca gtgttaaaat ctcaaaccga aaaatcggtt cgccggtcgt cgccaaccca
      721 accatcttga ttgcaatgaa tcgtccctcg ctcgataaat tcgaaaatga cgtcatttcc
      781 gatggattaa ttatttatga cgattcgatt attgatcgtg cacctctgcg ccaggacgtc
      841 cgcgttctgc caatcccggc tacgaaattg gctgacgaac tgggcaattc caaagccgct
      901 aatatgatta tagttggcgc tttgattgaa tatttaggat ttatgtcagt tgaagttgtc
      961 ctgaattcct tgggcgaagt attgagacaa aaaaatttag tcgagctgaa tcaaaaagct
     1021 cttctaaagg gtgtggattt cacgcgcaaa attaaatcct gaaatggact aacgcgtttt
     1081 tcgtttttct gattaaagtt cactttaata acacttttgt tataagctta tactcgataa
     1141 gagaaatttc ctctggcttt ctaattggtg ataataatgt tttcaaattt ttgtaattga
     1201 attaaagctg gggcattttt tgagattgcc gattctaccc gtaaatttaa tttgataata
     1261 gtacggttta atgaaattta tatttactac agatagaatt tttctacgga ttttgttgat
     1321 tgctattcta tccgttttta ttttagtaaa tagcctattg gcgcaaatgc tcggttccga
     1381 ccaaccgccg ccagatattc actggcgtca gatagaaaca cagcattatc ggataatttt
     1441 tcccgccgaa atcgaatcgg aaagccagcg gatggctaat accctcgagt atgtttactt
     1501 gcctttacgt aaaacactga aagcgaacag tcctaaatgg ccattagtcc tttccaaccg
     1561 cagtgccgtc gctaacggtt atgtaaccct tgccccgcgt aaaagtgaat ggtttacgac
     1621 tcccgatcaa tccgctatgt ccggcaatat tgaatggtat aatctcctgg ctacccatga
     1681 agggcgccat atggtccagt ttgatcgctt caattccgga tttacgcgct tggctggatt
     1741 tttgttcggt gaatatgcga ttctggggct ctcgttctgg catgcgccga tgtggttctg
     1801 ggaaggcgat gccgtcgcta tggaaactgc tttgactgaa tctggacgcg gtagccaacc
     1861 ggaattcgat ctgccgattc ggactttgtt aatcaatcaa aaactatatt cttatgataa
     1921 agcctatctt ggctcgtaca aggattatta tccgaattat tatcatctgg gctttttaat
     1981 ggtaactcat ggacgacggc agtatggcgc aaatatctgg gatcacgtat taaccagctc
     2041 gactcggcgc tcctggaatc catttggttt ctcgaaagct tgccgaatca ataccggtcg
     2101 caaaactaaa gagctttatt atgatacaat gactgaactc gattcactct ggtgcaaacc
     2161 gatcgaagga ctggaaatca cctctgccaa aaaagttaac ttaaaaccaa aaaaggtctg
     2221 gacacaatac cgctggccgc aatatgcgac tgatggctca atcatcgccg taattaatgg
     2281 cttaggtgac atcaatactt tagtgaagat caaccctgat gggcaggaaa ctgaaatagc
     2341 caaaatcgcc agcgccaatc ggttttcgac tgccgggaat ttgattgcct gggaagaatt
     2401 gacgcctcat ccacgctggg gcgccgcgat tttttccgat atagttgtct atgatttatt
     2461 agtcggacag gagcgtaaac ttaccaagaa cggtaaatac ttttcgccag cggttgcgcc
     2521 gcatgggcag atgatcgcgg cggtaaaatt taccccggaa agaaaatgca atctggttct
     2581 gcttaactct aacgatggtc aagaaatcag gactttaccc aatccggata acggttttat
     2641 caagaaccca gcctggtcgc ccgatggaag gcaattagtc tattttaaac aatctggatc
     2701 ctccaagtca ctggcgattt acgatctgga gaacaatact gaagagatta ttatcgcgga
     2761 atgtcattcg ttcgacgaat tcccaacctt ttatggcgat tatctgcttt ataattcagc
     2821 ctggtcggga attgacaata tttatgcggt ggaaattgac agcgggcgtc gttttcaagt
     2881 gacttcacgc aaatacggtg cctattaccc ggcggtttca gccgacggct cgatgatgct
     2941 ttttagcgat tatgatttta atggtctgga tgtatgcgca atgcacctcg agccagcccg
     3001 ttggcaggcg ctggaagcgg tcgaacgatg cgatattcgc tattatgaga cgctggttgc
     3061 tcaggaacaa ggtggtaata ttttcgaagc gggaaaaatc ccaaccagat cgtatccggt
     3121 ttcggattat gatccctatc ggcatttatt taattttcat agctggtact atttcttctc
     3181 agagacctct gccaatctcg gattattttc caatgatatt ctcaataatg ccgccgccaa
     3241 ttttgaattg aatttcgatg ctaacgagaa agccttttca gtcggatcgg aagtttgcta
     3301 tgccggtttt tatcctatcc tgagcgccgg atttaacctc ggcaagagaa tcagctattc
     3361 cggtggactt tttgacgaat taaccactga gacttggaac gaaacgtcat tctcattggg
     3421 tgcaaacttg ccgctggatt attctagcag tgtatttaac cggaagttga acctcggctt
     3481 gagtggtgct tatacctaca tcagtgataa aaccacagtc gaagattacg agattggtaa
     3541 cggtggcttt gtcccttttt cctatgaaat tgcctaccag aattaccaga ctccagcgat
     3601 tcgcgatgtc aagccgaccg ccggctatac cgttaaagcc aatttcaaac atactccact
     3661 gggtggcgat tatcaaagtc agatgtattc cttccaaggg aattattact tgcccggttt
     3721 ggctaaacat catagcctga tgctcaatgc caaagtggaa ctgcaaaatc ctgacaatta
     3781 ccgctttgcc agtctggtgc cgtttgccaa aggctacgac tatatttata acgaccggtt
     3841 ggcgggtgcc ggcgctacct acgaattgcc gctcgcctat ccggactggg cactgggttc
     3901 gctgttttac ctcaagcgcc tacggggtgc ctttttcggc gatttcaacg tgggaaaaac
     3961 cagtgacttc aaacagcaat ataattcagt gggtctcgac ctaaattttg actttaatct
     4021 tttacagctc gcggtcgaat taaatgccgg cgtacggctc gcctaccgtt tggcggaaaa
     4081 agacctacac gccagcttgc tgattttcaa catcgaattc tgagcgggta actaatctct
     4141 tctccgttta tcaaagactt tgatgattaa aactgagagt taatttatta tgaaaccaaa
     4201 cgacaatata aatcaggtta tgatcaaaat cgggggaatg cactgtgcaa tgtgcgtcaa
     4261 aaccgtcgca gatgcactca gcaaagtccc cggcgtccgt gcggtaaacg tcaatctcgc
     4321 caatgagaaa gcttacttag aatttgataa tggacatttt tcgatcgaag ccgctaaaag
     4381 agcgattgaa tccgtcggtt atcaatatct cggtcgggaa ggcgagcaag atttaatcga
     4441 acaggataaa accctgaatc gggaattgcg ctcaaggaag cgacgtataa ttattggttt
     4501 cattaccggc gtaattttaa tgattccaat gttcatcaaa attagcctgc cgtttccaat
     4561 tgcctatttg atgctggcgc tagctactcc ggtcttcatc tacctgagtt atccgatttt
     4621 ccgtgcggca gggcaggcgc ttcgtaacca cagtttgaat atggatgtaa tgtatgtaat
     4681 gggtatcggg gtcgcttttc tagccagcct actgggaact ttcgaaattg ttttgacacg
     4741 cgaatttatg ttttttgaaa cggctatttt cctggcgaca tttctaacgc tcggtcgcta
     4801 tctggaaacc cgcgccaaag gcaaaacgac agccgcaatc aaacgattga tcgggcttca
     4861 accccaaacc gtcatcgtta ttcgggataa tgtcgaaagc gaaattccgc tggaatcggt
     4921 tgtaattaat gatattgtag ttgtcaagcc cggcagtcgc gtacccgtgg atggagttgt
     4981 aattagtggt gaaagctata ttgatgaatc tatgttgacc ggtgaatccc tgccggtttt
     5041 caagcagacc ggcgccaagg tcgttggtgg aacgctgaat cagaacggca ttctacacat
     5101 tcgagccgaa cgaatcgggc gtgatacggt tctggcgcaa attattcgtc tggttgaaca
     5161 agctcagggc agtcgcccgc cggttcaacg actggcggat gtggcggtca gttacttcat
     5221 tcctgtgatt ttagccattg ccatattgtc ttttgggttc tggtattttg ttgctgagca
     5281 gacattgctt ttcgcgctta cagcgtttat ttcagtgctg gtaattgctt gtccctgcgc
     5341 actcggacta gcgactccca ccgccgtaac agtcggcatc gggcgtggcg ccgaacttgg
     5401 tattttaatt aaaaatggaa ctgttctgga agtagcaccg cgagtcacca cagtcgtttt
     5461 cgataaaact ggtactctga caaccggcaa accgttggtg actgacattt ttcccgtcgg
     5521 gatttctacc gcagaattac tgcgctatgc ggctgcggtc gagaaaaatt ccgagcatcc
     5581 gctcagcacg gcgattgtga attatgccaa ggaattggga attgcaattc cagaatcagc
     5641 caaattcgcg gcttatggcg gtcgcggagt tacagcttat gttgataatc gcgcagtttt
     5701 cagcggcacc gaattatttt ttcaggaaaa taaaatcaca atttcaccgg atattttagc
     5761 gcctgctgag aaatacaccg atgaaggcaa gtcaatagtt ttcgtagcaa ttgacgggca
     5821 agcagtcggc gttatcgcta ttgccgatac attgaaagat accgccgctt ccgctatcga
     5881 aaaattacgg aaagacggtt tgaaaatcgc gatgatcacc ggcgataacc gtcgcacggc
     5941 gcaagctata gcggcgcaag tcggaatcga aaccgtgatg gcagaagttc tgccgcagga
     6001 taaagctact gttattcgtc agatgcagac aagaggcgag gtcgtggcat tcgtcggcga
     6061 tggcatcaat gatgccgtgg cgctggcgca agctaatatc ggaattgcgg tcggttccgg
     6121 tagcgatgtg gcactcgaaa gcggcgatat tgtcctggtg aaaaatgatt tgaatgatgt
     6181 cagcgcggct ctgcaactca gtcgcaaagt tatgtcgcgc attcgccaga atcttttctg
     6241 ggcttttgcc tataatgcgg ctttggtgcc ggttgcggcg ggggcgcttt atccgctgtt
     6301 tggtattact tttcgaccgg aactgggtgg actggctatg gcactcagtt ctgtgacggt
     6361 catcagtctt tcactaaacc tgcggcgttt taatccggct cgataaactc tttcatttaa
     6421 tgcgctatta ttttaatatt tagtaatgaa agtaactggt tgcgatagat ggatctcaca
     6481 cagataaaaa ccattgccgc tggattttat atagtggatg agaatggtaa aaaacttccc
     6541 ggaaccataa aaattcccta tgcggaaaaa gacggtttta taagacatat ttcccaggtt
     6601 gaaaaaggac tggcttgcgg ctgttcctgt ccggtttgcc atcagccggt tatcgcgcgc
     6661 aaaggggcac ataaacgaca tcactttgcc cattaccccg acgcagtttg taatgccgaa
     6721 actgttctgc attaccttgc caagcgccta ttgttcgaaa aacttcagac tttattgaaa
     6781 cgaaaagtgg ctctcgtttt gcgttggaaa tgccaacttt gtggtgatca gcattccttc
     6841 aattttttcg aaaatgtcca ggctatagtt agcgaaaaaa ccttgagcga ctgccgcccg
     6901 gatctttgtc tggttgacca gcacggcgcg ccgacagctt tgatcgaaat cgtcgtggcg
     6961 catccaccgg acgaaaaagt tttaattttc gttagccgga aacaaattcc gctattgatt
     7021 ttccgaattc gctccggtga agaaatggaa gaaatggcta cttgcccaac gattaatccg
     7081 gatttgatca tccattgtcc acgtaggcgc tgtccggaat gcggtgctcc gctttttgag
     7141 aaatcgctct acattctgcc agcggtttgt tggcgttgtg gagctggaat gaatttagct
     7201 tttcttgata ttgacggaca ggttgccgga acggaggaat tttcagccaa ggatcgccag
     7261 ataatcagcc gaaataacgt tatcctgcga gacactatcc gtcctggtga ccgtaaacgc
     7321 accgttagtc atatctgtcc ccattgcgga gtagtcaccg gcaacgctta tttgcgttac
     7381 ctaaaacgtc acgagcaaag gcttctagtc caaaattccg gcaaatattg ccgccaatgt
     7441 cataggcatt ttgacgaata gttttctatc tgagctattt ggcgattatt tttttacgag
     7501 attcgagaaa ctgaaatcca gttccctatc agaataagaa tccggctcta aaatagatca
     7561 ccgccctgat ttctccaggc tggaaaacac tcttgtcgcc ccaacccagc actaaatcga
     7621 atggtccaag gggcgagaca tacttcagac cgataccgta acccaataga ttctgagact
     7681 cttttatggt ttcgtggtaa atattgcgga agccaatcgc cgtattgaca attgctttag
     7741 cttgcaaatt atgcaatagg ttgtaggcat aatccaaacg cagtatagtc atttcagaac
     7801 caaccagttg atcatattcg actcctacaa aagtttctgg cccaccgcga taaataaaac
     7861 ggttggtaga accgtccatc ttagcattcc cgtaaaaacc tgaaaaatgc aaagtgttgc
     7921 gtttgatgga ctgatatacg tcggctgaaa gttcaaaacg ccgatagttg gtatgccgac
     7981 caatttggga gattgtcttt tcgtaaacgc aatgaaccaa agcgcctttg cgcggcataa
     8041 atgcattatc cagaagatca atgttagaag acaactgaaa tttatatagg tcatcattcc
     8101 aatcataaaa tcgcaacggt tcatccggtg cgatatccgg tttgatatta attgattcat
     8161 aatccagctc tgcttcgaaa ttccagtaat ttttataaag caaaccgaaa ccgccaccga
     8221 agagcgctga acggtcaata tatgatgcga ttctattgcc gttaatgcta tagacatagg
     8281 ttggaatttc gcgataatgg gcgttgacaa atggataaaa aggcagttgg aagcgccgcg
     8341 agggataaag cgttttgagc ttaatctgat tcagtccaat gaactgccat tcgacatcga
     8401 gccttaatcc atgaactatg tttgaagttg tctggaaatt cagcgccgct accaaataat
     8461 ggaagtcatc atatcgcacc ccgaggcgca gtttctgagg tgtatgctcg acgacattca
     8521 gtattagatt gatcttacct tctccggctg gttcggtagt atagctgata ttattaaaat
     8581 aatccagac
//
LOCUS       DAOSMP010000066         8468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_8320_length_8468_cov_13.717104, whole genome shotgun sequence.
ACCESSION   DAOSMP010000066 DAOSMP010000000
VERSION     DAOSMP010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8468
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8320_length_8468_cov_13.717104"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..>2760
                     /locus_tag="PKX09_08880"
     CDS             212..>2760
                     /locus_tag="PKX09_08880"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOU17973.1"
                     /translation="MRSIFKKPEINNEKPAVLRGGRFSRLLILNISTCLLITNAQISI
                     AKTVIKPGKRLHFEKINTEKGLPSNHITSIVQDSIGFMWYATRENGLCRYDGNEIVTY
                     HPEDSVATSLTCQNIKRLFIDSKGALWIIGSVGDIPHKGCIDKYDPLTNGFIHILSPN
                     DSIPSIIDIAEDRLGNFYFSVTDIRQQNNQILKAFNQNGSYHIKKISPLVFDDNSVLP
                     ANYVIHRIINKGDGKLLLGLSQYGIVEYDINTGRVNEDDFQNLNYYFNRFSFKIDDIL
                     LDCNQNLWIAAHKFLAKYSIADGSLQYYLLPLKPDGSEINPYQDPIKNYYYFTINQLY
                     EGSGGQIWVVGSQCNQNETFFRYDPETEIFWHYSTDPYDENQISVNNINCFFIDKSGS
                     AWLATQYSGVFRFSLPRPFMDLRSISESINFNNDNVNDVLEDHENNLWFGLKGKVLRY
                     NPKTKNVKIFPLKLSNQESPPPGTLHIYEDSQNTIWVGTTLGLFKLNPSSQEFIYLNH
                     VTKNQPNKQNSIRANHIPAIYEDHEGNFWLGTAAGLCRYDRQTNQRKYYSISEPTKNV
                     PNKNIIYDILEDRNNNLWLATRNGLILFDRNSGTYQLISDSTLIFMPESDEGFYRLYE
                     AQDGQIWLTSSLPYLISYNPLTKKFVYRTEKTGYIWKYPITGIIEDHNGIFWFRTTQG
                     IIKHNPILNTFTLFDRNDNVNYYYKDRSTSDIREPSYLKNNILRNYCRLRSGWIMIGG
                     KCGYTYFHPDSINKKYVPNIVITKFKNDYTEKIISNHSPNQITLDLPWNENYFTISFS
                     LLDYYIPAENKYYYILERYDKDWKKCVKTNSVSYSKVPPGQYTFRVKGENS"
     assembly_gap    2761..2860
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2861..4491
                     /locus_tag="PKX09_08885"
     CDS             <2861..4491
                     /locus_tag="PKX09_08885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU17974.1"
                     /translation="KVQERTQELAEANVELNREINIRMKAEESLKRSEEVARVLLNAP
                     IDLAMLIDPDGNILAANNSVLKHFQASENEVINHNLSEFSPLNLFERRKIFLDKCIST
                     KLPQEFEDENRGRIFHYHLYPIIGMDQEVNNIAIYIRDITDQREMENFLRNARSELEK
                     QVNQRTAELRKTNRQLLKEINTRKKIENKLRTSEEKYRDLFQNANDLIWTMDKTGKFL
                     SVNNHFKNLSGYSKKELFAINPLTLIHPEHQFRIIRCYLKVLTDGPTGTELNVMTKSG
                     EIRPIWLKMRPIMKNNQIIGIHGIGRDITEIRHAQQELRESEEQKRESMRQFILKLAH
                     EVKNPLASIKSSAQLVASMNNNHNPQVEKHMDIINRNVDTCNKVIRDLFSYAHATNYQ
                     FEEVNAAKFIKSINDFIEEKLNEYPYIKISVNYDRDLPPIYIDEFRLTQAFQNIINNA
                     FEAMTKKGRFTLNVKALKTEKQVQFLFTDTGSGIPAEDLPQIFQSFYSSKSKGFGLGL
                     ALTKEIIEFHHGKISVNSILHHGTTFKVTIPAFQ"
     gene            4526..5932
                     /locus_tag="PKX09_08890"
     CDS             4526..5932
                     /locus_tag="PKX09_08890"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOU17975.1"
                     /translation="MNSILIVEDSQDTRENIAELLTMNGYKVRTTDNGQTALRIIKTQ
                     MPDCVILDVRLPDTTGHEILKQIQNEIESGLVVIIITAYGEVSMAVNAMKMGAYDYLE
                     KPFENAVLLMVVKRGLENQQVKRELAHLRQMLGTDQDSEQVLGHSPAIKKVISQINSV
                     AQTDLTVLIQGETGTGKEVAAHLLHNKSKRAKQPFIAIDCGAIPENLIESEFFGFEKG
                     AFTGATQSRPGKFQLAHQGTLYLDEIGNLPLAQQRPFLRVIENKTFRPIGAINESKVD
                     IRLIIASNANLLQLVSENKFRKDLYYRISEYTITMPPLRERLEDIPHLTALFLSEANF
                     ELNSKITDISEEAMAKLLSYNWPGNVRQLRNVIRKAALLATDTIKLSHISFPSEFDSE
                     EPEQSFFTVYKNDINRYPNYRTAYAEFTREFEKELIKNAYEKAHGNITKAARIYGIDR
                     RNFYHKLDKYAKDFPKKQ"
     assembly_gap    6048..6147
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6954..>8468)
                     /locus_tag="PKX09_08895"
     CDS             complement(6954..>8468)
                     /locus_tag="PKX09_08895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU17976.1"
                     /translation="LTKSLGNEIRARITIIDTTGNVIADSESDPRLMKNHRQRPEIKQ
                     ALRGNVAHSQRFSTTVEENMLYVASPLVQDGQTVAVVRTSIYLSHLNDIFKQILNRII
                     KIAILIIVIALSAAFLLARYYSKPVNQLVTAARQIGQGKLETRVNIQTSDEFAELADS
                     FNAMAVEINRLLEDVARQRDELQTIVAAVPSGLLVLTADGRVAFYNREFQKITEREEL
                     QDRFYWEILRQPQFTEFIKDIQASRANRTQEIVINNKTYLCSAEAETSSAEIVLIFSD
                     ISEQKAVQQIKRDFVVNVSHELRTPLTAIKGFVETLQEETSGNEHRYLTIIARHTDRL
                     INIVNDLLTLAEIEEKNQLEYETISLSNLINDTVKIFEERLQAKNLKMVVEIADGLTT
                     IKVDPFKFQQVLINLLDNAIKYTEQGQITIRVQKKADQVQIVIQDTGCGIPPEHLPRL
                     FERFYVVDKARSRQMGGTGLGLAIARHIVLLHNGTIEVESQPGVGTTFFINIPA"
BASE COUNT         2631 a         1802 c         1554 g         2281 t
ORIGIN      
        1 ttgatggttt gatgcataat aacctctcta attttttaaa taccttttga acgttggcta
       61 ataagagttt ttaaataatt ttgctggttg gttttctgat ttttagccac tctaatttga
      121 ataaaaatca catagaaagt tacattctat ctttacatcc tcaaaattat ttttaaatta
      181 agctggttgg aagataattc ggcttaacgc gatgagaagt atttttaaaa aaccggaaat
      241 taataacgag aaaccagccg tcctaagagg cggccgcttt tcgaggttat tgattctaaa
      301 tatatctact tgtttattaa tcacaaatgc ccaaatatca attgctaaaa cggtcatcaa
      361 acccggcaaa cgcttgcatt tcgagaagat taacactgaa aaaggactac ccagtaacca
      421 tatcacctcc attgttcagg attcaattgg atttatgtgg tatgccacca gagaaaatgg
      481 attgtgccgc tacgatggga acgagattgt tacttatcat cccgaagatt cagtagccac
      541 ttctctcacc tgtcaaaata ttaagcgatt atttattgat tcaaaaggtg cgctctggat
      601 tatcggttca gtgggcgaca tccctcataa aggttgtatc gataaatacg atccgcttac
      661 caacggtttc attcatatcc tatcgccgaa cgattcgatt cccagcatta ttgatatagc
      721 cgaagatcgt ttgggtaatt tctatttctc ggtcactgat atcagacaac aaaataacca
      781 aattttgaaa gcctttaatc aaaatggttc atatcacata aaaaaaatca gtcctttagt
      841 atttgacgat aattctgtat tgcctgccaa ttatgtaatt catcgaataa taaataaagg
      901 cgatggaaaa ttactgttag gtttaagtca atacggcata gtcgaatacg atataaatac
      961 cggccgggtt aacgaagacg acttccagaa tcttaattat tacttcaacc gtttctcttt
     1021 taaaattgat gatatattac tcgattgtaa tcaaaacctg tggatagccg cccataaatt
     1081 tctggcaaaa tattccattg ccgacggctc attgcaatat tatctattgc cgctcaaacc
     1141 ggacggcagt gaaataaatc cctaccagga tccgataaaa aattactatt actttaccat
     1201 caatcaatta tatgagggga gtggcggaca aatatgggta gttggaagcc aatgcaatca
     1261 gaacgagacc ttctttagat atgatccgga aacagaaata ttctggcatt attcgaccga
     1321 cccttatgat gaaaatcaaa tatcggttaa taatattaac tgttttttca ttgataaaag
     1381 cggttcggcc tggctcgcta ctcaatatag tggtgtgttt agattttctt taccacgccc
     1441 ttttatggat ttacgttcaa tatcggaaag cattaatttt aataacgaca atgtgaacga
     1501 tgttcttgaa gatcacgaaa ataacctctg gtttggtcta aaaggaaagg ttctgcgcta
     1561 caatccgaaa actaagaatg tcaaaatttt ccccctaaag ctctccaatc aagaaagccc
     1621 accaccgggc acattacata tttatgaaga ctctcaaaat acaatctggg tgggtactac
     1681 tcttggatta ttcaaactca atccatcttc tcaagaattt atctatttaa atcacgtcac
     1741 taaaaatcag ccgaataagc aaaattcgat tagagccaac catataccgg ctatctatga
     1801 agatcacgag ggtaattttt ggctgggtac cgccgctggt ttatgccgct acgatcgcca
     1861 aaccaatcaa agaaaatatt actcaatttc tgaaccgact aagaacgtcc cgaacaaaaa
     1921 tatcatttac gatattctgg aagatcggaa caataattta tggttagcga ctcgcaacgg
     1981 actcatttta tttgaccgaa atagcggtac ttatcaatta atttctgaca gtactttgat
     2041 ttttatgccg gaaagtgacg aaggttttta ccgtttatac gaagctcaag atggtcagat
     2101 ttggttgacc tcgtcgttac catacttgat ttcatacaat ccgctgacaa agaaatttgt
     2161 ctatcgtacc gaaaagacgg gatatatctg gaaataccca attaccggca taattgaaga
     2221 tcataatggc attttctggt ttagaaccac tcaaggaatc ataaaacaca atccaatatt
     2281 gaatactttt accctcttcg atcgcaatga caatgtaaat tactattaca aagacagatc
     2341 aacctctgac atcagagaac ccagctacct caaaaataat atattaagga attactgccg
     2401 actacgttcc ggctggatta tgattggtgg taaatgtggc tatacttatt ttcatccgga
     2461 tagcattaat aaaaaatatg tccccaatat cgttattacg aaatttaaaa atgactatac
     2521 tgaaaaaata atttccaatc attctcctaa ccagatcaca ttagacctac cttggaatga
     2581 gaattacttc accatcagtt ttagtttact ggactactat atccccgctg aaaataaata
     2641 ctactacatt ttagaaagat atgataaaga ttggaaaaaa tgcgttaaaa ctaatagcgt
     2701 tagctattct aaagtcccac ccggtcaata tactttccga gtcaaaggcg aaaattcgga
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaaaagtcca ggaaagaacc
     2881 caggaactcg ccgaagccaa tgtggaatta aaccgcgaaa tcaatattcg gatgaaagcc
     2941 gaagaatcct taaaacgcag tgaagaagtc gctcgcgttc tgctcaatgc tcctattgat
     3001 ctggcaatgt taattgaccc tgacggtaat atcttagccg ctaacaattc ggttttgaaa
     3061 cattttcaag cctcggagaa cgaggtaatc aatcataatc taagtgagtt ctcacctcta
     3121 aatttattcg agcgccgaaa aatattcctc gataaatgta tcagtaccaa actaccgcag
     3181 gaattcgagg acgaaaaccg gggacgaatt tttcactatc atctttatcc tattattgga
     3241 atggaccagg aggtcaacaa cattgccatc tatatccgcg atattaccga tcagagggaa
     3301 atggaaaatt tcctgcgcaa tgctcgatct gaactcgaaa aacaagtcaa tcagcgcacc
     3361 gcggaattaa gaaaaaccaa ccgacaactc cttaaagaaa tcaatacgcg taaaaaaatc
     3421 gaaaacaaac tccgaaccag tgaagaaaaa taccgcgacc tattccaaaa cgccaatgat
     3481 ctcatctgga ctatggataa aaccggtaaa tttctatcgg ttaacaacca ctttaaaaac
     3541 ttatccggct actctaaaaa agaattgttt gccatcaatc ccttaacttt aatccatccc
     3601 gaacatcaat tccgcataat tcggtgttat ttgaaagttc ttacagatgg tccaactggt
     3661 acagaactaa atgttatgac caaatccggt gaaattcgcc ccatctggtt aaaaatgcgt
     3721 cctataatga aaaataacca gatcatcgga attcatggaa ttggtcggga tattaccgaa
     3781 atacgacacg cccagcaaga acttcgtgaa tccgaagaac aaaagcgcga aagtatgcgt
     3841 caatttatct tgaaactagc tcatgaagtc aaaaatccgc tggcaagcat caaaagttct
     3901 gctcagttag ttgccagtat gaataacaat cataatcctc aggttgaaaa acatatggat
     3961 attatcaatc gcaacgttga tacttgtaat aaagtcattc gggatctgtt ctcctatgcg
     4021 catgccacaa attaccaatt tgaagaagta aatgccgcta aattcatcaa gtccataaac
     4081 gattttatcg aagaaaaatt aaacgaatac ccatatataa aaatttccgt gaattacgac
     4141 cgagacttac cgcccatcta tattgacgaa ttccgattaa ctcaggcttt tcagaacata
     4201 atcaataatg cgttcgaagc aatgactaag aaagggcgat tcactctcaa cgtcaaagcc
     4261 ttaaaaaccg aaaaacaggt tcaatttctg tttaccgata ccggctcagg aataccagct
     4321 gaagacttgc cgcagatttt tcagtcattt tacagctcca agtccaaagg tttcggactg
     4381 ggtctggcac tcaccaagga aattatcgaa tttcaccatg gtaaaatttc cgtaaatagt
     4441 attttacacc acggcaccac ttttaaagta actattccag ccttccagta atatgtttta
     4501 agacacttaa aatcaggatg ttattatgaa ttctatatta atagtcgaag attctcaaga
     4561 cactcgcgaa aacattgccg aacttttaac tatgaacggc tataaagttc gaactactga
     4621 caatggccaa accgctcttc ggataattaa aacccaaatg ccggattgtg taatcctgga
     4681 cgtacgcctg ccggatacaa ccggacatga aattctgaaa cagatccaga atgaaatcga
     4741 aagtggtctg gtcgttatta tcatcactgc ttacggcgaa gtctcaatgg ctgtcaacgc
     4801 catgaaaatg ggagcttacg attatctcga aaaaccgttc gagaacgcgg ttttattaat
     4861 ggttgtaaaa cgcggcttgg aaaatcaaca agtcaagcga gaacttgccc acttacgcca
     4921 gatgttggga accgatcaag acagcgaaca ggttctcggc catagtccgg caattaaaaa
     4981 agttatcagc cagatcaatt ccgttgctca gaccgactta actgtcctga ttcaaggtga
     5041 aaccggaacc ggtaaagagg tcgccgccca tctattacat aataaaagca agcgcgccaa
     5101 acaaccattt atcgcgattg actgtggcgc cataccagaa aatttaatcg aaagcgaatt
     5161 tttcggcttc gagaaagggg cgttcaccgg tgccacccaa agtcgcccgg ggaagtttca
     5221 actagctcat caaggtactc tttatctcga tgaaatcggc aatctaccgc ttgcacaaca
     5281 acggccgttt ctccgtgtca tcgaaaacaa gacctttcgc ccgatcggtg ccatcaatga
     5341 aagcaaagtt gatattcgcc taatcatcgc cagcaatgcc aatctgcttc agctggtcag
     5401 tgaaaataaa ttccgtaaag atttatatta tcgaatctcg gaatatacta tcacgatgcc
     5461 tcccctccgt gaaagacttg aggacattcc tcatttaacc gcattatttt tatccgaagc
     5521 taattttgaa ttaaacagta aaatcactga tatttccgaa gaggcgatgg ctaaattact
     5581 ttcatataat tggcctggca atgtccggca actgcgtaat gttatacgta aagccgccct
     5641 gctcgctacc gatacgatca agctctctca catttcattc ccctctgaat ttgattcgga
     5701 agagccggag caaagcttct ttacggtata taaaaacgat ataaatcgct acccaaatta
     5761 tcgaacggct tacgctgaat ttacccggga atttgaaaag gaattaatta aaaatgcata
     5821 cgagaaagct catggcaata ttaccaaagc cgcccgtatt tatggcattg atcgtcgtaa
     5881 tttctatcat aaactcgaca aatacgctaa agattttccc aaaaaacagt aacccaatct
     5941 acaaatagtt cgccctactt ggctaataag aatccttgat ttaagtcggc gaattagtca
     6001 ttggtcatta gtcattggtc attagtcatt ggtcattagt cattggtnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnatt agtcattggt cattagtcat tggtcattag
     6181 tcattggtca ttagtcattg gtaaaaaggc tgaggttgag aaaagaagag ccacactgtc
     6241 actctgagcc acactgtcac tctaaaccat actgtcactc tgcattacac tgtcactctg
     6301 agcctgtcga agagggacag catcgcttac tctaagtcta acggaagcca agaggcgacg
     6361 agagcatcct tcgacaagct caggatgaca ggaatagaca agctcaggat gacaggaata
     6421 gacaagctca ggatgacagg agcagacaag ctcaggatga cagattgtat gtcactctga
     6481 gcctgtcgaa gagggacagt atcgcttacc ttaagtctca cggatactag gagacgacga
     6541 actcatcctt cgacaagctc aggatgacaa aagatcgaca agctcaggat gacaaaagat
     6601 cgacaagctc aggatgacag attgtatgtc actctgagcc acactgtcac tctaaaccat
     6661 actgtcactc tgcattacac tgtcactctg agcctgtcga agagggacag catcgcttac
     6721 tctaagtcta acggaagcca agaggcgacg agttcatcct tcgacaagct caggatgaca
     6781 gattgtatgt cactctgagc ctgtcgaaga gggacagtat cgcttacctt aagtctcacg
     6841 gatactatga ggcgacgagt tcatccttcg acaagctcag gatgacagga atagacgagc
     6901 tcaggatgac aggaaaatat tcttcatgcc cttgtggttt tgcgaaagat tatctacgct
     6961 ggtatattaa tgaaaaaggt cgtaccgaca cccggttgac tctcgacttc gatcgtacca
     7021 ttatgaagta aaacaatatg tctggcaatt gctaatccca aaccggttcc gcccatctga
     7081 cgcgagcggg ctttatccac cacgtaaaac cgctcgaaaa gccgcggtag atgttccggt
     7141 ggaataccgc aaccggtatc ctgaataaca atttggacct ggtctgcctt cttttgcact
     7201 cgaatagtga tttgtccctg ctcggtatat tttatggcat tatccagaag attaattaaa
     7261 acctgctgaa atttgaacgg atcaacttta atcgttgtca agccatctgc gatttcgact
     7321 accattttta gatttttcgc ttgcaggcgt tcttcaaaaa ttttaacagt atcgttaatc
     7381 aaattcgata aactaatagt ctcatattcc agctgatttt tttcttcaat ttccgccagc
     7441 gtcagcaagt cattgacaat attgatcaag cgatcggtat gccgcgcgat aattgtgagg
     7501 tagcgatgct cgttgccgga tgtttcttct tgaagtgtct cgacaaagcc tttgatggct
     7561 gtcaatggcg tgcgcaattc gtgcgagaca ttgaccacga agtcacgctt aatttgttga
     7621 acggctttct gttctgaaat atcagaaaat atcagaacaa tttcagcgct ggacgtctcc
     7681 gcctcagcac tgcacaaata ggttttatta tttataacta tttcctgtgt gcgattagcc
     7741 cgacttgcct gtatatcttt gataaactcg gtaaactgtg gctgacgtaa aatttcccag
     7801 tagaacctgt cctgtagttc ctcccgttca gtaatttttt gaaattcacg attataaaat
     7861 gccacccgtc catcggcagt cagtaccagt aaaccggaag gcaccgccgc aacgattgtc
     7921 tgcaattcat cacgttgtcg ggcaacatcc tccagcaagc gattaatctc aactgccatc
     7981 gcgttaaaac tatcagccag ttcggcaaat tcatcactgg tctgaatatt aacacgggtt
     8041 tcgagtttgc cctgcccaat ttgacgagcg gctgtgacca attgattgac cggtttggaa
     8101 taataacgcg ccagcagaaa agccgcactc agcgcgatta caattataag tatagcaatt
     8161 ttgataattc gattgagaat ttgcttgaaa atatcgttca aatggctcaa ataaatgctc
     8221 gtacgcacga ctgctacagt ctgtccatct tgaaccagcg gtgaagcgac gtacagcata
     8281 ttttcttcaa ccgtcgtgct aaaacgttga gaatgcgcca cattacccct caaagcctgc
     8341 ttgatttccg gtcgctggcg atggtttttc atcaggcgcg gatcagactc agaatcggca
     8401 atgacattac cggtggtatc aataattgtg atgcgagccc ggatttcatt cccaagcgac
     8461 ttggtcag
//
LOCUS       DAOSMP010000067         8282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_8588_length_8282_cov_18.925368, whole genome shotgun sequence.
ACCESSION   DAOSMP010000067 DAOSMP010000000
VERSION     DAOSMP010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8588_length_8282_cov_18.925368"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..468
                     /locus_tag="PKX09_08900"
     CDS             265..468
                     /locus_tag="PKX09_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17977.1"
                     /translation="MDIYESAITALKIPFFPLGLRFPARDVLKAVMVNWMVSFSAPLL
                     LFSQSLFSTSLLFTNDQLPMTND"
     gene            complement(506..1642)
                     /locus_tag="PKX09_08905"
     CDS             complement(506..1642)
                     /locus_tag="PKX09_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HOU17978.1"
                     /translation="MKRREFIGTLGIAAATARISQAFPFISQLKTKTTDSGMKMRYEI
                     KTLKLKHTWTISRNSSDVKDNVFVYLEKDGITGIGEAAPNIRYDETPESTIELIKKAT
                     PLIERLNPSEFTQFGFELKEIESGQTAGKCAIDMALMDWIGKSLNLPLYKFWGLDPTK
                     APLTSFTIGIDTAEIVKQKTREAEPFKILKIKVGGPNDEEIITTVRSVAPDKPIRVDA
                     NEGWKDKEIATRKIEWLAKNGIELVEQPLPSEMLEETRWIRERIDIPIIADEAVKTAS
                     DIPKLADAFDGINIKLMKAGGLQEALRMIWLAKSLGLKIMIGCMIESSVAISAATQLC
                     SLIDYADLDGNLLISNDPFSGAIFRDGRQFPADRPGLGVRKATG"
     gene            1820..3037
                     /locus_tag="PKX09_08910"
     CDS             1820..3037
                     /locus_tag="PKX09_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU17979.1"
                     /translation="MEEVRFKVYPYRWVVLIAFMVIIAVNQLCWITFAPITGRAAQFY
                     GVSDLSIGFLSMIFMIVYIVISIPASWIIDTYGIKIAVGIGAALTGVFGLIRGLLATS
                     YQYVLIAQIGLAIGQPFILNSITKVAARWFPIAERATASGLGSLAMYVGIIIGMALTP
                     YMTIRTGIEGTLLLYGVASVIGAVIFFVLVRERPPTPPCPAGQEERALVFDGLKQMLK
                     LKEFQRLMLVFFIGLGVFNGVTTWIENIVRPRGFSITQAGLTGGLMIIGGIIGAVIVP
                     MLSDRVRKRVPFIIQALVGATLGFIGVTFARSYWLLLTSAFVMGFSLLGVGPLGFQYG
                     AEISYPAPEGTSNGLLLLMGQISGVLFIFGMDSLKSPQTGSMTIPLVILIALMVLCTL
                     LSVNLKESNLLRK"
     gene            3185..3622
                     /locus_tag="PKX09_08915"
     CDS             3185..3622
                     /locus_tag="PKX09_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17980.1"
                     /translation="MKKLVLSAVVVMFLMASLSYGFGLGIMLGEPSGLSGKYWMSEKN
                     AIAGGLAWSLSHDYMHVHADYLIHNNNLINVSGTTLPFYYGLGAGIGFGDDFHLGARI
                     PVGIEYFFADRKFDVFFELVPVVGLLPDFGFGVSGAIGLRYNF"
     gene            3806..4087
                     /locus_tag="PKX09_08920"
     CDS             3806..4087
                     /locus_tag="PKX09_08920"
                     /inference="COORDINATES: protein motif:HMM:NF021390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HOU17981.1"
                     /translation="MKNGVITWRTETGEKIVTRFYSLSDNSLKFVKVVLPGGKEYTLP
                     QVVSASGVRYTDEREIVFWMKGETAFIQQRAPSGEWQILYNNCRVITEP"
     gene            complement(4244..5545)
                     /gene="thrC"
                     /locus_tag="PKX09_08925"
     CDS             complement(4244..5545)
                     /gene="thrC"
                     /locus_tag="PKX09_08925"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOU17982.1"
                     /translation="MLYYSTTDRSHQVSLKEAVLKGMPPDGGLYLPESLPVMPDKFFQ
                     DLPEMTFQSVSHEIAKALLGGDVPSDVLQKIILEALNFETPLVAISEKIKTLELFHGP
                     TLAFKDVGARFMSRLMAYFIRNDCRELTILVATSGDTGSAVANGFFQVPGINVIVLYP
                     AGKISLIQEKQIATLGENITALKVEGTFDDCQRLVKTALVDPEVQSVLTVSSANSINI
                     ARLIPQTFYYFNAIRQIPNFHSRKIAICVPSGNFGNLTAGLIAKKMGLPVIRFIAATN
                     INHVVPDYLATGKYEPRASIQTISNAMDVGNPSNFTRMLALYDYSVEKMRRDLAGCYF
                     NDDETRAAIAEVYRNSSYILDPHGAVGYLGIQNYLARHPEIDTGIFLETAHPAKFCDI
                     VEPVIGATVPIPDRLKKFLERPILSRAISSDYDEFKDLLRA"
     assembly_gap    5582..5681
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5682..6468)
                     /locus_tag="PKX09_08930"
     CDS             complement(<5682..6468)
                     /locus_tag="PKX09_08930"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOU17983.1"
                     /translation="MKSVTVFAPATVANVACGFDIFGFAVKEPGDVAKVTLRENPGVE
                     ITRITGDNGKLPRTAEKNTAGFAALRYLETIQSQQGVSIELHKKMPLSSGLGSSAASA
                     VAVLFAINTIFDNRLTKSELLSIALQSEKLACGAAHADNAAPALFGGFLLIRENNPPD
                     IVELPVPENLYCTIIHPEIEINTADARNILSPNVPLKDAVKQWANTAGLVAGLYRKDL
                     TLISRSMNDYIIEPQRAKLIPHFYELKKAALDRGALGCSISGSG"
     gene            complement(6468..>8282)
                     /gene="thrA"
                     /locus_tag="PKX09_08935"
     CDS             complement(6468..>8282)
                     /gene="thrA"
                     /locus_tag="PKX09_08935"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HOU17984.1"
                     /translation="AEEIQIWKDVNGFMSADPSKVKEAFSLTELSYEEALELSHFGAK
                     VLFPPTIQPLIDKNIPIRIMNTFNPDFPGTVIRKNPSANGGFVKGVTSIRDIALLTVQ
                     GSGMVGVSGISSRLFSALAKVNVNVILISQASSEHSICVAILRESVNIAKKAIEEEFA
                     LEIKLRLVDPVVVENDLVIIAVVGERMRRTTGLAGRLFSALGEKSINVIAIAQGSSEL
                     NISIVIDQKDEKQALNAIHNAFFAKKIKMNLFLFGVGLIGSTLIEQIKTNYSNLLENY
                     GLDIKVVGLANSRKMLIEPDGIDLNNWRNSLNECQVKNDMRVLLAEIKKLNLDNNVFI
                     DCTASADIVNYYEKILAGRTSIVTANKIGNTGPYAQYVKFHELAKSKHVHFLYETNAG
                     AALPIISTLRDMINSGDQIIRIEAILSGTISYILNNFKPGKKFSAIVREAKARGFTEP
                     DPRDDLSGIDFARKVLLLVREIGIPAELSDIQIEPLLPENCLKAPTVEAFFTELEKSN
                     DYFDKLLQQASAEGKVLRYIAIYENEKAELKLVQVEANHPFYFLSGSDNIVAFTTKRY
                     AENPLVIKGAGAGAEVTAAGVMADIFKIANAVMKSKSF"
BASE COUNT         2106 a         1684 c         1969 g         2423 t
ORIGIN      
        1 atggttagtt ttagcgctcc tctgctcctc ttttctcagt ctcttttctc aacctcttta
       61 ctatttacca atgaccaatt accaatgact aatgaccaat gactaatgac caactttcct
      121 gttctttttt cctatgccga aaaaagaacc aaaaaaggca acggctaaag aacatctcac
      181 gggggttcgc tcagtcccag cgaatggaat ttaaggcgca aaagttatca agttcatata
      241 atgttagtga tataggaaag ggtgatggat atttacgaaa gtgctattac agcgctgaaa
      301 attccatttt ttccgctggg acttcgcttc ccagcccgag atgttcttaa ggccgtaatg
      361 gtaaactgga tggttagttt tagcgctcct ctgctcctct tttctcagtc tcttttctca
      421 acctctttac tatttaccaa tgaccaatta ccaatgacca atgactaatt accaatgacc
      481 aatgaccaat gaccaatgac cattctcagc ctgttgcttt tcttacacct aaacccggtc
      541 tatccgccgg gaactggcgg ccgtccctga aaatcgcacc gctaaaggga tcgttcgaga
      601 ttagcagatt accatcgagg tcagcgtaat caatcagcga acagagttga gttgcggcgg
      661 aaatggcgac cgaactttcg atcatacaac cgatcataat tttcaagcca agtgatttag
      721 ccagccagat cattcgcagg gcttcctgaa gaccaccggc tttcatcagt ttgatgttaa
      781 tgccatcgaa agcatcggcg agtttaggga tatcagaagc agtcttgacg gcttcgtcgg
      841 cgatgatcgg aatatcaatg cgctcacgaa tccagcgtgt ttcttccagc atctcggacg
      901 gcagaggttg ttcaactagc tcgatgccat ttttggctaa ccattcaatt ttacgagtgg
      961 cgatttcctt atctttccag ccttcattgg cgtcaacccg gattggttta tccggggcga
     1021 cactgcgcac ggtggtaatt atctcctcgt cgtttggacc gccgactttg atcttgagaa
     1081 ttttaaaagg ttcggcttcg cgcgtttttt gtttgacaat ttccgccgta tcaatgccaa
     1141 tcgtaaagga ggtcaaggga gctttagttg gatcgagtcc ccagaattta taaagcggga
     1201 ggttaagaga cttgccgatc cagtccatta aagccatatc aatggcgcat ttcccggccg
     1261 tctgtccgga ctcgatttct ttcagctcga agccaaattg ggtaaattcc gagggattta
     1321 atctttcgat caaaggtgtg gcttttttga tcagctcgat agttgattct ggagtctcat
     1381 catagcgaat attcggtgcg gcttcgccga taccggtgat gccgtccttt tcaagataaa
     1441 caaaaacatt gtctttcaca tcgctggaat tgcgtgaaat tgtccaggta tgtttcaatt
     1501 tcaaagtttt gatttcgtaa cgcattttca tgccagaatc ggtggttttg gtttttaatt
     1561 gagatataaa ggggaatgcc tgtgatatac gtgcggtcgc ggcagcaatc cctaaagtac
     1621 cgataaattc tcgtcgtttc atttcgatcc tgttaaaatt taaatccgat tacgttgcaa
     1681 tttaatctct ttatataact gagcaaagtt cttttggtgt aaataattct acctaaagct
     1741 tgtgtcataa ttctaaatgt cataaaatca gtcgaattta tggcgcaatt aatttccaat
     1801 tcatcggagg atttgccaga tggaagaagt tcgatttaaa gtatatcctt atcgttgggt
     1861 agtgttaatt gcatttatgg ttattatcgc ggtgaatcag ctctgctgga tcacatttgc
     1921 gccaatcact ggtcgagccg cgcaattcta tggggtttct gacctcagta tcggtttttt
     1981 atctatgatt tttatgatcg tgtatattgt tatttcgatt ccggcttctt ggattattga
     2041 cacgtacgga attaaaatcg ccgtcggaat cggcgccgcc ttaactggag tattcggttt
     2101 gatacgcggt ttgttggcga cgagttatca atatgtacta attgctcaaa tcggactcgc
     2161 cattggccag ccattcattt tgaattccat tacgaaagtt gcggcgcgtt ggtttccgat
     2221 cgcagagcga gcaacggctt ccggtttggg ttcgctggcg atgtatgtcg ggatcatcat
     2281 cggaatggca ttaacgccgt atatgacaat tcgaaccgga attgagggca cgttattgct
     2341 atatggagtt gcctcggtta tcggggcggt tatttttttc gtgctggtta gggaacgtcc
     2401 gccgacgccg ccctgtccgg ccggtcagga ggaacgggct ttggttttcg atggcttaaa
     2461 gcaaatgctg aagctaaaag aatttcagcg actgatgttg gtatttttta tcgggctcgg
     2521 agtttttaac ggagtaacta cttggatcga aaatattgtc cgtccacgcg gtttttcgat
     2581 tactcaagcc ggcttgaccg gtggtctgat gattattggt gggataatcg gggcggtaat
     2641 cgttccgatg ctttccgatc gggtgcgcaa gagagtgccg tttatcattc aagcgctggt
     2701 gggagcaacg ctgggattta tcggtgttac ttttgcgcgg agttattggc tattattgac
     2761 ttcggctttc gttatgggat tttccctgtt gggagtaggt ccgttgggat ttcaatacgg
     2821 ggctgaaatt tcctacccag ctccggaagg aacctcgaac ggattattgt tgctaatggg
     2881 acaaatttct ggggtgctgt tcattttcgg aatggacagt ttgaaatcac cgcagaccgg
     2941 ctcgatgacc attccactcg taattttaat cgctctgatg gttttatgta ctttgctcag
     3001 cgttaatctg aaggagtcga atttgctccg gaaataaaaa aatgcatccg attcaaaaac
     3061 caactataaa aattttaatt tttattgtta ttttaaggac gaatattaat tgctattgtc
     3121 attgttgatt tttatatttt ttgcagtatt ttaaaacaga gaaaaagaaa agaaaggagt
     3181 cattatgaag aagttagttt taagtgcagt agttgtgatg tttttaatgg ctagcttaag
     3241 ttacggattc ggcttgggta ttatgctggg tgaaccttcc ggtttgagtg gcaaatactg
     3301 gatgagtgaa aagaatgcga tcgctggtgg gttagcctgg tcattgagtc acgattatat
     3361 gcatgtgcat gcggattacc ttattcataa caacaactta attaatgtta gcggtactac
     3421 tcttccattt tattatgggt tgggcgcagg tattggtttt ggcgatgatt ttcatctcgg
     3481 ggcacggatt ccagtaggga ttgaatattt ctttgctgat cggaaatttg atgtcttttt
     3541 tgaattggtt ccggtagtgg gcttattgcc tgattttggt tttggggtta gtggtgcgat
     3601 cggcttgaga tataatttct aacaggcgat tttaaaaaat tatgaaaaat agccgtgagg
     3661 ataaatttct ccggctattt tttaattgtt gagaatctga acataggctc tatttttccg
     3721 tatgaatatt aagagcaaag tactattaat ttttactatt tctttgatat gtagttgttc
     3781 gccaaatcaa gatcagttga aggttatgaa aaatggtgta attacctggc gaactgagac
     3841 cggcgagaaa atcgtgacgc gattttattc tttatcggat aatagtctga aattcgtcaa
     3901 agtggttcta ccgggtggta aagagtacac attgccacaa gttgtatctg cttccggtgt
     3961 gcgctatacc gatgaacggg aaatcgtgtt ctggatgaag ggcgagacgg cctttatcca
     4021 gcaacgcgct ccgagtggtg aatggcagat tttgtacaat aactgccggg tgataacgga
     4081 accgtaaaat ccatttttta ggttctcaag cctgacgaga ggctaagatt aaaactataa
     4141 gatacgagat ctgatagaac tggttaaaaa aaacttctaa aggtttttcg atacatattt
     4201 tattgtccac aattttattt tcaaccttct gaaggttgat tgactatgcc cgcaggagat
     4261 ccttgaattc gtcgtagtcg ctggaaatag cgcgcgacag aatcggacgc tccaggaatt
     4321 ttttcagtcg gtcggggatg ggaactgttg ctccgataac tggttcgaca atgtcgcaga
     4381 acttcgccgg gtgagctgtt tccagaaaaa ttccggtgtc aatttctggg tggcgggcga
     4441 gataattttg aataccaagg tagccgacgg cgccatgggg gtcgaggatg taactggaat
     4501 tgcgatagac ttcggcgatg gcggcgcgag tttcatcgtc attaaaataa cagccagcca
     4561 aatcgcgacg cattttctcc accgaataat catagagcgc cagcatccgg gtgaaattgc
     4621 tgggattgcc aacgtccata gcgttggaga tagtctgaat tgaggcgcgt ggttcatatt
     4681 ttccggtcgc tagataatct ggcacgacgt gattgatatt ggtagccgcg atgaagcgta
     4741 ttaccggtaa gcccattttt ttagcgatta atccggcggt caggttaccg aaattgccgc
     4801 tcggtacgca gatagcgatt ttcctagaat gaaaattggg aatctggcga attgcgttga
     4861 aataataaaa ggtttgagga atcaagcggg cgatgttgat cgaattggca gaactgacgg
     4921 taaggacact ctgcacctcc ggatccacca gagcggtttt gacaagacgc tggcagtcgt
     4981 cgaaggtgcc ttctactttt aaggcagtaa tattctcgcc gagagtcgca atctgttttt
     5041 cttgaattag gctaattttt ccggctggat aaagcacgat gacattgatg cccggcacct
     5101 ggaaaaaacc attagcaacc gcactgccgg tatcaccgga ggtggcaact aagatcgtga
     5161 gttcgcgaca gtcattacga ataaaataag ccatcaggcg cgacataaaa cgagcgccga
     5221 catctttaaa ggcgagggtt ggtccgtgaa aaagttcgag tgttttaatc ttttccgaaa
     5281 tagccaccag cggcgtttcg aaatttaaag cttcaaggat aattttttgt aggacatcag
     5341 atggaacatc gcctccaagt agagctttag caatttcatg agaaaccgac tgaaaagtca
     5401 tttccggcaa gtcttggaaa aatttgtccg gcataaccgg caatgattcc ggcagataca
     5461 gcccgccatc cggtggcatg cctttcaaca cggcttcttt cagagaaact tggtgagagc
     5521 gatcagtagt actgtaataa agcaaatttt atccttcttg agcgatgatt ttgggacctt
     5581 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngaccggagc cggaaatact
     5701 gcacccgaga gcgccgcggt ccagagccgc ctttttcagc tcataaaaat gaggaataag
     5761 tttcgcccgc tgaggttcga taatataatc attcattgat cggctaatca aggttaggtc
     5821 tttccgatag agcccggcaa ccagtccggc ggtgttagcc cattgtttta cggcgtcttt
     5881 tagaggaaca ttgggagaaa gaatattgcg ggcatcggca gtattgattt caatttctgg
     5941 atgaataatc gtgcaataga gattttccgg gaccggtaat tctacgatat caggcggatt
     6001 gttttcccga attaaaagaa aaccgccaaa gagagccggt gcggcgttgt cggcgtgggc
     6061 ggcgccgcaa gccaattttt ccgattgcag agcgatactg agcagttcgg atttcgtcag
     6121 ccggttatcg aagatagtgt taatggcaaa gagtaccgcc accgcactgg cggcgctcga
     6181 tcccagtcca ctactcaatg gcattttttt atgtaattcg atggaaacgc cctgctggga
     6241 ttgaatggtt tccagataac ggagggcggc gaatccggcg gtgttttttt cggcggttct
     6301 cggcaatttc ccgttgtcgc cggttatgcg tgtaatttcg acgccgggat tttcgcggag
     6361 agtaacctta gcaacgtcgc ccggttcctt aacggcaaaa ccgaaaatat cgaaaccgca
     6421 agcgacatta gccacagtag ctggtgcgaa aacagtgacc gacttcatca aaaactctta
     6481 cttttcataa cagcgttggc gattttaaaa atgtcagcca ttacaccggc ggcggtgact
     6541 tccgccccgg cacccgcgcc tttgataacc aacggatttt cagcgtagcg tttcgtagtg
     6601 aaagcgacga tgttgtcact accgctcaag aagtagaagg gatgattcgc ttccacctga
     6661 actaatttca attcagcttt ttcattttca taaattgcga tatagcgtaa tactttacct
     6721 tccgcagagg cttgctggag caatttatca aaatagtcat tggatttttc gagttcggta
     6781 aaaaacgctt ctaccgtcgg agctttgaga caattttccg gcaaaagcgg ttcgatttga
     6841 atgtcggaga gttcggcggg aatgccgatt tcgcggacga gcagtaaaac cttacgggcg
     6901 aaatctatcc cactaagatc gtcgcgtgga tcgggttcgg taaaacctct ggctttggct
     6961 tcgcgcacaa tagcgctaaa ctttttcccc ggtttgaaat tattgagaat atagctaatt
     7021 gtgccagaaa gaatcgcttc gattcggatg atttggtcgc cggaattgat catatcgcgc
     7081 agagtgctga taattggcag agccgcaccg gcatttgttt cgtagagaaa atggacgtgt
     7141 ttacttttcg ccagctcgtg gaattttaca tattgagcgt acggaccagt gttgccgatt
     7201 ttattggctg ttacaatgga ggtgcgaccg gctaaaattt tttcgtaata attcacgata
     7261 tcggcgctgg cggtgcaatc tatgaagaca ttgttgtcga gattgagttt ttttatttct
     7321 gccagtaaaa cccgcatgtc atttttgacc tgacactcat tgagagaatt gcgccaattg
     7381 ttgaggtcaa ttccgtccgg ctcgatcagc attttccggc tattggcaag accgacgact
     7441 ttaatatcca gcccataatt ttccagtaga ttggaataat tggttttaat ctgttcgatt
     7501 aaagttgatc cgattaaacc caccccgaag agaaataaat tcattttaat ctttttagcg
     7561 aagaaagcgt tgtgaatggc attaagggct tgtttttcgt ctttttggtc aattacgata
     7621 gaaatattca gttccgagga accttgagcg atggcaatga cgttaatgga tttttctccc
     7681 agtgcgctaa aaagacgtcc cgccaagccg gtagtgcgac gcattcgctc tccaacaacc
     7741 gcaatgatga ctaaatcatt ttctactacg accggatcaa ccaggcggag tttaatttca
     7801 agggcgaatt cttcttcgat ggcttttttt gcgatattga cagattctcg caaaatggca
     7861 acgcagatag aatgctcaga cgaggcttgg gaaatcaaaa tcacgttgac gttgactttt
     7921 gccagtgcgc tgaaaagacg agaagagatg ccactaacgc cgaccatgcc actaccttga
     7981 accgtcaaca atgcaatgtc gcggattgaa gtgacacctt tgacgaagcc gccgttagca
     8041 gagggatttt tgcgaatgac cgttccggga aaatccggat tgaaggtatt cattatacga
     8101 ataggaatat tcttatcaat tagcggctga atagtgggtg gaaatagtac tttggcgcca
     8161 aagtgactca gttccaaagc ttcttcatag ctgagttccg ttaatgaaaa agcttctttg
     8221 actttgcttg gatcagccga cataaagcca ttgacgtctt tccagatttg aatctcctcc
     8281 gc
//
LOCUS       DAOSMP010000068         8260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_8623_length_8260_cov_16.043266, whole genome shotgun sequence.
ACCESSION   DAOSMP010000068 DAOSMP010000000
VERSION     DAOSMP010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8260
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8623_length_8260_cov_16.043266"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1070
                     /locus_tag="PKX09_08940"
     CDS             126..1070
                     /locus_tag="PKX09_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOU17985.1"
                     /translation="MKAKTVSTLLLMTGLLQIATGQITDAEGKLKTKSTDEHTGWKIG
                     GVTALNLAQTSLTNWAAGGENSLALNGLFSAFANYKKGKNVWDNSLDLGYGVLKQGKE
                     ADFMKTDDKIDFLSKYGREAFTNVYYSALFNFKTQMAPGYNYPNDSVKISNFLAPAYL
                     LAAIGMDYKPNAYLSTFIAPATGRLIIVNDELLSNAGAFGVEPGKTTKAEFGGYLRAI
                     YTRNDFKTELLKNLSFTIKIDLFSDYLDHPQNIDINWETLVAFKVNQYISVNFTSHLI
                     YDDDTKISIDKDNDGVIDEFGPRVQFKEILGVGFSYKF"
     gene            1086..1511
                     /gene="mscL"
                     /locus_tag="PKX09_08945"
     CDS             1086..1511
                     /gene="mscL"
                     /locus_tag="PKX09_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014704667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOU17986.1"
                     /translation="MKVKAKPQSPKLLQEFKAFAMRGNVIDMAVGIIVGAAFGKIVTS
                     VVNDIIMPPIGLLIGGVNFTDLKIVLKHAVDGNPAVTLNYGNFLQVVFDFLIIAFAVF
                     MLVKALSAAKREEEAAPPAPPAPTKTEILLTEIRDLLKK"
     gene            1608..1862
                     /locus_tag="PKX09_08950"
     CDS             1608..1862
                     /locus_tag="PKX09_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOU17987.1"
                     /translation="MKCRKGCGACCIAISISSPLPGMPNGKPAGVRCIHLTVENYCKI
                     HDQPDYPAVCRNLKPSTEMCGESFDEAMKYLIKLEKLTSS"
     gene            2197..2643
                     /gene="tnpA"
                     /locus_tag="PKX09_08955"
     CDS             2197..2643
                     /gene="tnpA"
                     /locus_tag="PKX09_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOU17988.1"
                     /translation="MATYTQIIYQLVFSTKNRECTLTPELRPELYKYIWGILKNKKCH
                     LYRIGGVSDHVHIITHIHPTVAVAPLIKDIKIASSQYIKTITPDSKFTHWQIGYGAFT
                     YSIKDKDRLIEYVKNQGNHHRKKTFQAELVKLLHEHGIEFDEKYLL"
     assembly_gap    2961..3060
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3215..3661
                     /gene="tnpA"
                     /locus_tag="PKX09_08960"
     CDS             3215..3661
                     /gene="tnpA"
                     /locus_tag="PKX09_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOU17989.1"
                     /translation="MATYTQIIYQLVFSTKNRECTLTPELRPELYKYIWGILKNKKCH
                     LYRIGGVSDHVHIITHIHPTVAVAPLIKDIKIASSQYIKTITPDSKFTHWQIGYGAFT
                     YSIKDKDRLIEYVKNQGNHHRKKTFQAELVKLLHEHGIEFDEKYLL"
     gene            complement(4228..4716)
                     /locus_tag="PKX09_08965"
     CDS             complement(4228..4716)
                     /locus_tag="PKX09_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17990.1"
                     /translation="MKKQNYISRRSFLRRLGVLFVSIPFMLSAFSCKNQENLSESGSF
                     DQYLKNLPSITLDNASGMEGSSEVFKVAKTAVIEGLNNQGVVTKIIYTNDTINLESII
                     VKVLNSEDEYYYITEDTTANQPTGRLANGTVLLNDVKNSIKALRDFNKAELKSRYIVI
                     QK"
     gene            4865..5695
                     /locus_tag="PKX09_08970"
     CDS             4865..5695
                     /locus_tag="PKX09_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17991.1"
                     /translation="MRKNYYVIVAVILACVFAAFLFGELFAFSPIIVGFQKFEFPNTI
                     VYIQSRAQIPDWSNCDTLTIPVEKVLALKFKSKPEFFIFRDSLSYVRRSPTYSRLCAI
                     PPNRILIAPWSLKEADLGIIPLDIYLKHELAHILIFQQTGFMRAFRFPKWLSEGIAVY
                     AANQMGTGWYPSQEETYNYIRQGNFLPPRDYKTHRASQAKINVPNRSAFLYCEFACIV
                     DYLIETRSREKFQNYIESLFTESNHDEVFRRIYGIEFDYFVDEFKNHCIIQTENFSTQ
                     "
     gene            5873..>8260
                     /locus_tag="PKX09_08975"
     CDS             5873..>8260
                     /locus_tag="PKX09_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOU17992.1"
                     /translation="MSLIISLFFSLVLLCPSLFAGFEFIGNVKSQKVFPNRVEFKADN
                     ALFNIYVYDDHIIRFRYTNQKEFSSAPSYAVIAALPAQSAFTFKDEGKNFTLTTKALT
                     VRITKSPCRVAIFDSAGNLINADEEAFGVSFDNDEVRCHKKLLPGENFYGLGEKSDDL
                     CKTGKEYILWNTDFWAYDDRTDPLYISIPFFYGIRDFKAYGILFDNTCKSHFNFGASN
                     NRFYWFGAEKGEMDYYFIYGPEIKKVLTAYTNLTGRAELPPLWALGYQQCRWSYYPES
                     EVRNLAATFRRKKIPCDVIYLDIDYMDGYRVFTWNKKYFPEPAKMLADLKKDGFKVIP
                     IIDPGVKADTNYFGAREGLKQDLFVKYPDGQYYQGEVWPSWAYFPDFTKATTRQWWGD
                     KLNLLLKDGVAGFWNDMNEPTVWGTHFPDIVQFDGNGLKTDHKSARNIYALEMARATR
                     AGLRRYSPERHFILTRAGFAGIQRYSAVWTGDNVASELHLKIACRMSQNIGLSGVPFV
                     GSDVGGFVGNPSERLYIRWMQLGTFTPLFRQHSAWDTHRKEPWIFGSEVENTVRNFIN
                     LRYQLLPYFYSEFYTANQTGWPLMRPLFFNFQDDPECYRTEAQNQFVLGENLLVAPVL
                     SETDEFKRLYLPAGNWYDLNRHRFYTGKQWLIVEAPLNQIPYFLRQGGFLPMQAVQQY
                     VGEKPMTATELLIYPADESSYTLYEDDGLSYDYEKGVFILTKFTCRNSTNTCNIIIER
                     QKSDFQPQRQHYLFKIISTQPPVRVSVGDNQLLTCKSADDLTQQAEGFYYSTAEQM"
BASE COUNT         2426 a         1904 c         1588 g         2242 t
ORIGIN      
        1 accgcagagt gtgtaaagca ttttagagat tatttttagt taagtatttt ccgaaatgaa
       61 ttcctggcgt cctttgcggt taaaaataaa ttttcttaaa attgccaaca gttaaaggag
      121 gaattatgaa agcaaagact gtttcaacgt tactgttaat gactggcctt ctccagatag
      181 ctaccggtca aatcactgac gctgaaggca aactgaaaac caagtccacc gacgagcata
      241 ccggctggaa aatcggcggt gtaacagcgc taaatttagc tcaaacatcg ctaaccaact
      301 gggcggctgg tggcgaaaat tcactggcac tcaacggcct ttttagcgca tttgccaatt
      361 acaaaaaagg caaaaacgtc tgggacaatt ccctggatct cggatatggc gttcttaaac
      421 agggcaaaga ggctgatttt atgaaaaccg atgataagat tgatttttta tcgaaatatg
      481 gccgagaagc cttcactaat gtctattatt cagctctttt taatttcaag acccagatgg
      541 caccgggtta caattatccc aatgattcgg taaaaatttc caatttttta gcaccggctt
      601 accttttagc cgcaatcggt atggattaca aaccaaacgc ctatttgagt acttttatcg
      661 cacctgccac cggacgatta atcatcgtca atgatgaatt actgtccaat gccggagctt
      721 ttggagtcga gcccggaaaa actaccaagg ctgaattcgg tggttatctg cgtgcaattt
      781 atactcgcaa tgattttaaa accgaattac taaaaaattt atcttttacc attaaaattg
      841 acctcttttc ggactatctg gatcatcctc agaatattga tattaactgg gaaacgttgg
      901 ttgcttttaa ggtaaaccag tatatctcgg tcaatttcac ctctcacctg atttacgacg
      961 atgacacaaa gatctcaatt gataaggata atgatggagt aattgacgaa ttcgggccgc
     1021 gggtgcaatt caaagaaatt cttggcgttg gcttttctta caaattctaa ctatagagga
     1081 taaatatgaa agtaaaagca aaaccacaaa gcccaaaact actgcaggag tttaaagctt
     1141 tcgcaatgcg cggcaatgtt attgatatgg cagttggtat cattgtcggt gccgctttcg
     1201 gcaagatcgt cacttcggtt gttaatgaca ttattatgcc accgattggc ttattgatcg
     1261 gcggcgttaa ttttaccgat ttgaaaatcg tattgaaaca tgcggttgat gggaatccgg
     1321 cggtgacgct aaattatggc aattttctgc aggttgtatt tgatttcctc atcatcgctt
     1381 ttgcggtttt tatgctggta aaagccctca gcgccgctaa acgggaagaa gaagccgcac
     1441 caccagcgcc tcccgcccca actaaaacag aaatacttct gacggaaatc cgcgacttac
     1501 tgaaaaaata aagtctaacc cggtaccgca aaaatatact aatagaaact atttatttac
     1561 atctatctaa accatccata gcaattactt attcttagga ttattaaatg aaatgccgta
     1621 aaggatgcgg cgcttgctgt attgctattt ctatttcatc gccacttcct ggtatgccaa
     1681 acggcaaacc ggctggggta cggtgtattc acttaaccgt agagaattat tgcaagattc
     1741 acgatcagcc agattatcca gctgtttgcc gcaatttgaa accttccact gaaatgtgcg
     1801 gtgaatcttt tgacgaggct atgaaatatt taataaaatt ggaaaaattg acatcttcat
     1861 aaataactca ggattatatc ctgagtcata gaaatgcgaa acaccctcca accccgcagg
     1921 ggttgaacct gaataactca ggattatatc ctgagtccaa aaacgccaat aactacgcaa
     1981 ccccaacggg gttgaaccta agacctgaac aatccgtaac tccgtcagga gttgaatttg
     2041 cttaactcag gattatgtcc tgagtcataa aaatgcgaaa caccacacaa ccccgcaggg
     2101 gttgaacctg aataactcag gattttatcc tgagtccaaa gacctaaccc cgaaccccct
     2161 ccaaccccgc aggggttgaa caagaaggag gaaaacttgg ctacctatac ccaaatcata
     2221 tatcaattgg tttttagtac caagaaccgt gagtgcaccc ttactcctga attacgtcct
     2281 gaactttaca aatacatctg gggtatcctg aaaaataaga aatgtcacct ttatcgaatc
     2341 ggtggcgtct cagatcatgt ccatattata actcatatac atccgacggt cgccgtcgcc
     2401 ccattaataa aagatatcaa aattgctagt tcgcaatata taaaaaccat cacgcccgat
     2461 tccaaattca cgcattggca aatcggttac ggagctttca cttattccat aaaagacaaa
     2521 gaccgtttga tagaatacgt caagaatcag ggaaaccacc atcgtaagaa aacctttcag
     2581 gcagagttgg tgaaattgct acatgaacat ggaatcgaat ttgatgaaaa atatctgtta
     2641 taattcaatc ccgaccagtc gggattgatt attttgatcc cacataaatc tattcgattt
     2701 caatcccgtt gggattatga aaataacctt aaacagattc ggttcgatta tattattgtc
     2761 gggattggaa aatatacgaa aaatacgata ataaattgcg acaaacaact ccgtcaggag
     2821 ttgaacttgc ttaactcagg attatatcct gagcctaaag acctaaccct gaacaacctc
     2881 caaccccgca ggggttgaac ctgaataact caggattatg tcctgagtca taaaaatgcg
     2941 aaacaccaca caaccccgca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 taactcagga ttatgtcctg agtcataaaa atgcgaaaca ccacacaacc ccgcaggggt
     3121 tgaacctgaa taactcagga ttttatcctg agtccaaaga cctaaccccg aaccccctcc
     3181 aaccccgcag gggttgaaca agaaggagga aaacttggct acctataccc aaatcatata
     3241 tcaattggtt tttagtacca agaaccgtga gtgcaccctt actcctgaat tacgtcctga
     3301 actttacaaa tacatctggg gtatcctgaa aaataagaaa tgtcaccttt atcgaatcgg
     3361 tggcgtctca gatcatgtcc atattataac tcatatacat ccgacggtcg ccgtcgcccc
     3421 attaataaaa gatatcaaaa ttgctagttc gcaatatata aaaaccatca cgcccgattc
     3481 caaattcacg cattggcaaa tcggttacgg agctttcact tattccataa aagacaaaga
     3541 ccgtttgata gaatacgtca agaatcaggg aaaccaccat cgtaagaaaa cctttcaggc
     3601 agagttggtg aaattgctac atgaacatgg aatcgaattt gatgaaaaat atctgttata
     3661 attcaatccc gactggtcgg gattggaaaa tatacgaaaa atacgataat caattgcgac
     3721 aaacaactcc gtcaggagtt gaacctgaat aactcaggat tatatcctga gtccaaaaac
     3781 ctaaccctga acaacctcca accccgcagg ggttgaaccc gagacctgaa caatccgtaa
     3841 ctccgccagg agttgaatct gcttaactca ggattatgtc ctgagtcaaa agaagcagaa
     3901 cattacgcaa ccccgacggg gttgaataat gctatcagat catataagcg aatattatta
     3961 tcggtgattt cacctgcttt caatgcaggc gcactgacac aagaataagg agggagtagg
     4021 ttttaatctt aatattattc catataaagc gcttcaaccg gacatatggg aatacacgca
     4081 ccgcacccta cacagcaatt ctgatcagga aaccaacaat gacctttcaa agaatatctg
     4141 aagcatctat attgacaaac ttctacacac gcaccgcaac caatacactc ttcgcattta
     4201 actttcgctt tttccatagc atttagatta tttttgtatg acaatatatc ggctttttaa
     4261 ttccgcttta ttgaaatcgc gaagtgcctt gatcgaattc ttaacatcat tcaagagaac
     4321 tgtaccatta gcaagacgac cagtaggttg attagctgtt gtatcttcgg tgatgtaata
     4381 atattcatct tccgaattaa ggactttaac gattatagat tcaagattaa tggtatcatt
     4441 agtataaatg attttagtta ctactccctg gttgttcagc ccttcaatta ccgccgtctt
     4501 ggcgacttta aatacttctg aagatccctc catacctgaa gcattatcca gggtaatact
     4561 tggcagattt ttcaaatatt gatcgaaaga tccagattct gagagatttt cttgattttt
     4621 acaggaaaat gctgataaca taaagggaat actcacaaat agtactccca gacgtctaag
     4681 gaaacttcgt cttgaaatgt aattttgctt tttcatctta cctcctaatt ttatctcata
     4741 atacataata aatataataa aaaaattata aaatcaacaa aattttattt tctgctctcg
     4801 acaccccagg cgtcgagaac caaaaagtta cataagtgaa tccgggcttt taacaaaacc
     4861 taatatgcgc aaaaattact acgtgatcgt tgcggtaatt ttggcatgcg tctttgccgc
     4921 ttttttattt ggtgaactat ttgccttttc gccaattatt gttggatttc agaaattcga
     4981 gtttcccaat accattgttt atattcagag tcgggcgcag attccagact ggtcgaactg
     5041 cgacacgctc acgatacctg tcgaaaaagt gcttgcctta aagttcaaaa gcaaaccgga
     5101 gttctttatt ttccgcgata gtttaagtta tgtcagacgt tctccaactt actcccgact
     5161 ttgcgcgatt ccacctaacc gtattcttat tgcaccctgg tcgttgaaag aagccgattt
     5221 gggtataatt ccattagata tttatctgaa acatgaactc gcccacattc tcatcttcca
     5281 gcaaaccgga tttatgcggg catttcgctt cccgaaatgg ctctcagaag gcatcgcggt
     5341 ttacgccgcc aaccagatgg gcaccggctg gtatccatcc caggaggaaa cctataatta
     5401 catccgtcaa ggaaatttct taccgccgcg tgattataaa acccaccggg cgagccaagc
     5461 caaaatcaat gtccccaacc gctccgcctt tctttattgt gagtttgctt gcatcgttga
     5521 ttatttaatc gaaacgcgta gccgggaaaa attccaaaat tacatcgaga gcttatttac
     5581 ggagagtaac cacgatgagg tattccgcag aatttacgga attgaattcg actacttcgt
     5641 tgacgagttc aagaaccact gcattattca gacggaaaat ttctcaacgc agtaaatgcc
     5701 tgatgttact aattattatt atcctatact aatcctatct agtttttaaa aaaacttgct
     5761 ccagatttac tactctgcgt ttctcatatt ccatcttccc cattttctta accataacta
     5821 taacattatc cacaaactca actttaatct taacagcgaa aggtgcttaa ctatgtcact
     5881 aataatttct ttatttttct cgcttgtgct actttgtcca tcactttttg ccggttttga
     5941 atttatcggc aatgtcaaat cacagaaggt ttttcccaat cgggtcgaat tcaaagccga
     6001 caacgccctg ttcaacatct atgtttacga cgaccacatc attcgttttc gatatacgaa
     6061 tcagaaagaa ttttcatctg cgccgtcgta tgcggtaatc gccgctctgc ccgcccaatc
     6121 ggctttcacg tttaaagacg aaggtaaaaa tttcacactg accaccaaag cactgaccgt
     6181 gcgaatcacc aagtcgcctt gccgcgtcgc cattttcgat tcggctggca atttgatcaa
     6241 tgccgatgaa gaagctttcg gcgtcagctt cgacaatgac gaagtgcgtt gccacaaaaa
     6301 gctcctgccc ggtgaaaatt tttacgggct gggcgaaaag agcgacgatc tttgcaaaac
     6361 cggcaaggaa tatatcctct ggaacaccga tttttgggct tatgacgacc gcaccgaccc
     6421 gctttacatt tcaattccct tcttttatgg gattcgcgat ttcaaagcct atggcatttt
     6481 atttgataat acctgtaaat cgcacttcaa tttcggcgcc agcaataatc gcttctactg
     6541 gttcggtgcc gaaaagggcg agatggatta ttatttcatc tatggtcctg aaattaaaaa
     6601 agtcctgacg gcttatacca atctgaccgg acgggctgaa ctgccgccgc tctgggcact
     6661 cggctatcag caatgccgct ggagctatta tcctgaatcg gaggtacgca atctggcggc
     6721 gacttttcgc cgtaaaaaaa tcccctgtga tgtgatttat ctcgacattg attatatgga
     6781 cggctatcgg gtctttacct ggaacaaaaa atattttccc gaaccggcta aaatgctggc
     6841 tgatttaaaa aaggacggct tcaaagtcat cccgataatt gacccgggcg tcaaagctga
     6901 cacgaattat tttggggcgc gcgaaggcct caaacaggat ttattcgtca aatatcctga
     6961 cggtcaatac tatcagggcg aggtctggcc ctcctgggca tatttcccgg attttactaa
     7021 agccaccacg cgccaatggt ggggcgataa actcaattta ctgctcaaag atggcgtcgc
     7081 cggtttctgg aacgatatga atgagccgac cgtttggggc acgcattttc ccgacattgt
     7141 gcagttcgat ggcaacggtt taaaaaccga tcacaaaagc gcacggaata tttatgcgtt
     7201 ggaaatggca cgagcgacgc gcgcaggttt gcggcgctat tctcccgagc gtcattttat
     7261 cctgacacgg gctggatttg ccggtattca acgctactca gccgtctgga ccggcgataa
     7321 cgtcgccagc gagttgcatc tgaaaattgc ctgtcgtatg tcgcaaaata ttggcttgag
     7381 cggcgtaccg ttcgtcggta gcgacgtcgg cggcttcgtc ggcaatcctt cggaaaggct
     7441 ttatatccgc tggatgcaac tcggcacttt taccccactc ttccgccaac actcagcctg
     7501 ggatacgcac cgcaaagagc cctggatttt tggctcggaa gtcgaaaata ccgtacgcaa
     7561 tttcataaat ttgcgctatc aactgctacc ctatttttat agcgaattct atactgccaa
     7621 ccaaaccggc tggccactga tgcgcccgct gtttttcaat tttcaggacg atccggaatg
     7681 ttaccgcaca gaagcccaaa atcaattcgt tctgggtgaa aatctactgg tggcgccggt
     7741 gctttccgaa acggacgagt tcaagcggct ctatctgccg gccggtaatt ggtatgattt
     7801 gaatcggcat cgcttctaca ctggcaaaca atggctcatc gtagaggcgc cacttaatca
     7861 gattccgtat tttctgcgtc aaggcggctt cctgccgatg caagctgtcc agcagtacgt
     7921 cggcgaaaaa ccgatgaccg caaccgaatt gctaatctac cctgccgacg agagttccta
     7981 tacgctttat gaagacgacg gactttccta cgattacgaa aaaggcgtct ttattctaac
     8041 caaattcacc tgccggaatt ctactaatac ttgtaatatc atcattgagc gccagaaatc
     8101 ggactttcaa ccgcaacgcc agcattacct gttcaaaatc atctccactc agccgccagt
     8161 cagagtcagc gtcggcgata accaacttct cacctgcaaa tcggctgacg atttaacgca
     8221 acaagcggaa ggtttttatt attccaccgc cgaacagatg
//
LOCUS       DAOSMP010000069         8082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_8885_length_8082_cov_20.283668, whole genome shotgun sequence.
ACCESSION   DAOSMP010000069 DAOSMP010000000
VERSION     DAOSMP010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8082
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8885_length_8082_cov_20.283668"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="PKX09_08980"
     CDS             <1..564
                     /locus_tag="PKX09_08980"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOU17993.1"
                     /translation="AKLGWVTYSGPMVSSDLGQSLSSFSAEWLWRMITSNPYPVELTN
                     PLAQPLQAFQPGSAEGVLIGGCLSLITPLLGTAFMPDLKGVILIIEDVDEKTYRLDKQ
                     LQILRLHGVFDQIAGLIVGQFVNCFPSNPKRSFTVEDYLSNALKDTHFPVITNCAYGH
                     LKQRFTIPIGIRARISTNPPRLTLLGL"
     gene            696..911
                     /locus_tag="PKX09_08985"
     CDS             696..911
                     /locus_tag="PKX09_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU17994.1"
                     /translation="MTKPEVQINPEWCKSCEICVKFCPRNVLEMGRFTAQVVHPENCN
                     GCKICESLCPDFAIVVTVLEKEKEEAR"
     gene            908..2059
                     /locus_tag="PKX09_08990"
     CDS             908..2059
                     /locus_tag="PKX09_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOU17995.1"
                     /translation="MTRSGFKGSSSRIFMMQGNTACAEGALAAGCRFFAGYPITPSTE
                     IAEVLARRLPQIGGNFIQMEDEIAAMGAVIGASLAGIKSMTATSGPGFSLKQENIGFA
                     IMAEIPCVVVDVQRGGPSTGLPTHVSQSDVMQARWGTHGDHSIIALCPSTVRETYDLT
                     IKAFNCAERYRTPVMIMTDEIVGHLSEKVELPDPASLEIVNRQRPTVPPSDYLPYQMT
                     ASDIPPMANFGEGYRYHVTGLAHDETGSPTNDPAQIEQQILRINRKIERYRDQIVEVE
                     EFETQDMEILVIAYGATARSAKKAVSLAREAGLKAGLLKLKTLWPFADIEIEALAKSV
                     NKIIVPELNLGQIAHEVEWTVRRAKPVIRVNRINGEPITPAEILAAIKN"
     gene            2068..2883
                     /locus_tag="PKX09_08995"
     CDS             2068..2883
                     /locus_tag="PKX09_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOU17996.1"
                     /translation="MDYLYYLRTKNLPHIWCDGCGNGIVLKSLLRVINKLGWDKNDIA
                     LVSGIGCSSRTPGYVDFNTLHTTHGRALAFATGLKIANPKLHVIVVSGDGDATAIGGN
                     HFIHAARRNIDITMIIFNNMIYGMTGGQVSPTTPAGKMATTAPYGNPDPPFDISRLAA
                     AAGASYVARSTVFHYARLDKYIEQALTRKGFAVVEVLTPCPTSYGRMNKEGNGAAMLL
                     YQKENTVSLAKASGMSPEELRNKLVVGVFVDRQQPDYQSHYEAVVTKARSEIK"
     gene            2895..3455
                     /locus_tag="PKX09_09000"
     CDS             2895..3455
                     /locus_tag="PKX09_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOU17997.1"
                     /translation="MSFHYEIRLSGAGGQGLILVGKVLAEAAAIYDGLNATQSQSYGP
                     EARGGASRSEVIISDGDIDYPKAENIDLLLALTQEACDKYIADLKPDGILIVDSQMVT
                     KIPDGNFKVVALPIIQSAVERLGKFVVSNIIALGVIYKLAGIVSEDAIKNAIRARVPK
                     GTEELNLKAFQTGIELAEEWLKEHAR"
     gene            3480..6488
                     /locus_tag="PKX09_09005"
     CDS             3480..6488
                     /locus_tag="PKX09_09005"
                     /inference="COORDINATES: protein motif:HMM:NF019935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU17998.1"
                     /translation="MKSAIHYSLTDLLNRASDLGNHHRKFIHNIEMNFDNLFALINNE
                     HLTDQFHLLRFSPHPAREMKLIADLVANRETLLEMLGCYFSIQLLLLNFKAIDVLKMK
                     LSTTTNHHAVYRSFLLRTGNDFRLLTAAYMNTLLDIFLPRAQRPDFVICGVGTRVDQD
                     DIDLGIVDTGPANRAILTNAFGAMSNEMLKYACSLHFHLSEHVGTKGYTASIEDYHNL
                     LDHQIQDFVLISEMVNAVPILGNRELFDRFKNEILDRYYYSPDRDNKYHEGFLRGLLG
                     EIRDLMYKEPPENLLNPKRDGLRMLKALIFALKTWKRVEGNTSLEVLDILLVKERFNH
                     ENYYQVFQALTFLETFRFLYQLFVVQEEEIHLDSDEMQLSLQKVAYAMGYQDHSYALA
                     CTQMLTHYYDHHKIARLGTERLVEGLTEHLRQITIFYPITHFQSRAKSKAPLPDNIPL
                     YFMQTARFFQGVRFWDDLLSSLDNPNQILLKRFLADFMKLSEARRAVLMKAYVKWGVK
                     TPYTMISLFSIIADHQPKIVETQFFQEFIRDFIYNLEPTLENVSRLCQILNLAPQMMN
                     NFLSILPVELLTRLIAVVEEPYWKPEITSLRDTLLMLCRIYRSSSYYFKRFVQRAFNT
                     YAEYITSLTNQPKLNQLAEGLFRNIDNFDSLNEKITRLGDYYDFEFMRLGIALLDGAP
                     FDKINREFTNFSDNYLQMLFELCQEEVAAADQKEIPEMHDLLAIFTAGGHARGQAFDD
                     DYDLIVLLNSDSPAILDFGNKVIQKMNKYILRRSIIPQFRFADRFRRFVTTFSDLRRL
                     FEQPDENTFIDQSQLLGARLVIGSDYFLKVFQSEIITPFIFQRKAEFIETMLNEMRSR
                     HNYYNSTKIEVPEIKEGPGGLRDFENFIFILKAYFEWAEPLTETLFSRIESAITNDDS
                     NFIALVRDYQLLKHIRDLYRLSVSNDDHLDKAYLDQLVRPFCLAHGLELENGAELAEF
                     IQRIMRKNVERINILLRTLGLPEIGALPGK"
     gene            complement(6622..7422)
                     /locus_tag="PKX09_09010"
     CDS             complement(6622..7422)
                     /locus_tag="PKX09_09010"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU17999.1"
                     /translation="MSNIQEIKVRSILSQSKLPGVDFVINPYRGCQLACVYCYACFMQ
                     KFDGHSEPWGTYVDVKSNALEILTRQLAKCHNQTVYLSSVCDPYQPVEAEYQLTRRIL
                     KQLTLAQPQLTVQTKSALVIRDLDLLTQFKNCSVGMTIVTTDENLRREIEPGASPIAD
                     RIDALRRLKAAGLETYVFVGPILPYLTGWKQIVLQTREFVDYYMFDHTNPSGRIWSHI
                     KSWLIAKHPELLSSYEKILFGRSDYWWRVSRAIQDFCDKENINHQIFF"
     gene            7605..>8082
                     /locus_tag="PKX09_09015"
     CDS             7605..>8082
                     /locus_tag="PKX09_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18000.1"
                     /translation="MKTSHTRAFLCILIAFLMVTVSLTAAGNIATYNGKEIRSNRIVV
                     VNNSILKKLPAKPDQTMRLESARQAYQGRIVRNLPTEGIAEWEIKGDMRKTLDALNAI
                     DGISAFPNFVFKRENLQPKPIQKDALVPNDYFISLQWALNNDGTFDSLYTSMGGMPV"
BASE COUNT         2194 a         1762 c         1818 g         2308 t
ORIGIN      
        1 gctaaactcg gttgggtgac gtatagcggg ccaatggttt cttctgattt gggacaaagt
       61 ctcagctctt ttagtgccga atggctctgg cgtatgatta ccagtaatcc ttatccggtc
      121 gaactgacta atccattggc tcagccattg caggcgtttc aacctggttc cgccgaaggg
      181 gttttgatcg gcggatgtct ctctttaatt acgcccctgc tgggaacggc atttatgcca
      241 gatttaaaag gagtgattct gatcatcgaa gatgttgacg aaaaaaccta tcggcttgac
      301 aagcaactgc aaattttacg cttgcacggc gtttttgatc agattgccgg attgattgtt
      361 gggcagtttg tcaattgttt tccgtcgaat cctaaacgtt cgtttacagt ggaagattat
      421 cttagtaacg ccttgaaaga tacccatttc ccggtcatta ccaattgtgc ctacggacat
      481 ctcaaacagc gttttacaat tccgattgga atccgagccc gaatcagcac caatcctccg
      541 cgtctaacac ttctcggttt atgaagcaaa ttgattcagt caaccgaatt gcctatcaaa
      601 gcaccgccag tctcaagatt tctgggtaac aattcgactt aatttattaa gtttatgagg
      661 ttgtttttct caataggtaa aaaaggagaa tcgccttgac caaacctgaa gtccaaataa
      721 atccggagtg gtgcaaatcc tgtgaaattt gcgtcaaatt ttgcccccgc aatgtactcg
      781 aaatgggtcg gtttacagcc caggttgtcc acccggaaaa ttgcaacggg tgtaagattt
      841 gcgaaagtct ttgtccggat tttgccatcg tcgtcacagt tcttgaaaaa gaaaaggagg
      901 aagcgagatg accagatccg gttttaaggg ttcgagttct cgaattttta tgatgcaggg
      961 taataccgct tgtgcggaag gcgccctcgc cgctggctgt cgtttttttg ccggatatcc
     1021 gatcacgcct tctaccgaaa ttgcggaggt gctggcgcgc cgtctgccgc aaattggggg
     1081 caattttatc cagatggaag acgaaatcgc cgcgatgggg gctgtgattg gggcttctct
     1141 ggctggtata aaatctatga ccgctaccag cggcccggga ttttctttaa agcaggaaaa
     1201 tatcgggttt gcaattatgg ctgaaatacc gtgcgtggta gtggatgttc agcgcggcgg
     1261 tccaagcacc ggattgccga ctcacgtctc ccagagtgat gtcatgcaag ctcgctgggg
     1321 gacgcacggg gaccattcca ttattgcatt gtgtccttcg actgtccgtg aaacctatga
     1381 tttgacgatc aaagctttca attgtgccga acgctatcgg acgccagtaa tgataatgac
     1441 tgatgaaatt gttggacact tgagcgaaaa agtcgaatta ccggatccgg cttcgctcga
     1501 aattgtcaat cgtcaacggc cgaccgttcc gccgtcagat tatctacctt accagatgac
     1561 ggcttcggac attcccccaa tggcgaattt cggagaaggc tatcgctacc atgtcaccgg
     1621 tttggcacat gacgaaactg gctcaccaac caatgatcca gcccagatcg aacagcagat
     1681 attgcgcatt aaccgtaaaa tcgaacgtta tcgcgatcaa atcgtcgagg ttgaggagtt
     1741 tgagacgcaa gatatggaga tccttgtcat tgcctatgga gcgactgccc gctcggctaa
     1801 aaaagccgtc agcctcgctc gcgaagctgg ccttaaagcc ggtctattaa agctaaaaac
     1861 gctctggcct ttcgctgata tagaaatcga agcgctcgcc aaatccgtga ataaaatcat
     1921 tgtaccggaa ctgaatctgg gtcaaattgc tcacgaagtc gaatggacag ttcgccgcgc
     1981 taaaccagtc atccgggtca atcgcatcaa cggcgaacca attacaccag cggaaattct
     2041 ggcggcgatt aaaaattaga gggaagtatg gactatttat attatttgcg cacaaaaaat
     2101 ttgccccaca tttggtgcga cggctgtggt aacggcattg tgctgaaatc gctcctgcgg
     2161 gtaatcaata aactcggttg ggataagaat gacattgcgc tggtatcggg catcggttgt
     2221 tccagccgaa cgcccggtta tgttgatttc aatactttac atacgactca tggtcgggcg
     2281 ttggcttttg ccaccggctt gaaaatcgct aatccaaaat tacacgtaat cgtggtttcc
     2341 ggcgacggcg atgcgactgc gatcggcgga aatcatttta ttcatgccgc ccgtcgcaat
     2401 attgatatca ctatgataat tttcaataat atgatttacg gaatgactgg cggtcaggtt
     2461 tcgccgacga cacctgccgg taaaatggca acaaccgccc cttacggcaa tcccgatcca
     2521 ccttttgata tcagtcgttt ggcggcggcg gctggtgcaa gctatgtggc tcgttcaacg
     2581 gttttccatt acgctcgcct cgataaatac atcgagcaag cccttacccg gaaaggcttt
     2641 gccgtcgtgg aagttttaac gccctgcccg acttcttatg gacgaatgaa taaagaaggc
     2701 aatggcgccg ctatgttgct ttatcaaaaa gaaaataccg tgtcactcgc aaaagccagc
     2761 ggaatgagcc cagaagaatt acggaataaa ctggttgtgg gagttttcgt tgatcgtcaa
     2821 cagcctgact atcaatcgca ttacgaggca gttgtgacta aagcccgatc agaaattaaa
     2881 taaggagcaa aattatgtca tttcattatg aaataaggtt gagtggtgct ggcggtcagg
     2941 ggctgatttt ggtgggaaaa gtcttagccg aagccgccgc gatctatgac ggtttgaatg
     3001 ctactcaaag tcaatcctac ggtccggaag cacgcggtgg agccagccgc agtgaagtta
     3061 tcatctcaga cggcgatatt gattatccga aagccgagaa tattgacctg ttactggcat
     3121 tgactcagga agcctgcgat aaatatattg ccgatctgaa accggatgga attcttatcg
     3181 tagattcgca aatggtaacc aaaattcccg atggcaactt taaagttgtg gctttgccca
     3241 taattcaatc ggccgtcgag cgcctcggta aatttgtggt ttccaatatc atcgctctcg
     3301 gtgtgattta taaattagcg ggtattgttt ccgaagacgc tatcaaaaat gctatccgcg
     3361 cccgcgttcc gaaaggcact gaggaattga atttgaaagc ctttcaaacc ggcatcgagc
     3421 tggctgaaga atggcttaaa gaacatgctc gttaagccat tcaattttcg tcccgaacga
     3481 tgaaatcagc gattcattat tctttgacag accttctcaa tcgagcttcc gatctgggga
     3541 atcatcaccg gaaatttatc cacaatattg agatgaattt cgataatttg tttgcattaa
     3601 ttaataacga acacctaact gaccaatttc atttattacg tttttcgcca caccccgccc
     3661 gcgaaatgaa gctgatcgcc gatttagtag cgaatcgaga aaccttactg gaaatgctgg
     3721 gttgctactt ttctatccaa ttattattat tgaatttcaa ggcaattgac gtcttgaaga
     3781 tgaaactaag cactactact aatcaccatg ccgtttatcg gtcgtttctc ttacgtaccg
     3841 gtaacgattt ccgtttgctg accgcggcat atatgaacac cttgctcgat attttcctgc
     3901 cgcgcgctca aagacctgat ttcgtcattt gtggcgttgg tacgcgagtt gaccaggatg
     3961 acattgatct gggaattgtt gatactggac cggcaaatcg agcgatttta accaatgctt
     4021 tcggagcgat gagtaacgaa atgctcaaat atgcctgttc acttcacttt catctttccg
     4081 agcatgttgg caccaaaggc tatacggctt caattgagga ttaccacaat ctactcgacc
     4141 accaaattca ggattttgtg ctgattagtg aaatggtcaa tgcagttcca attctcggta
     4201 atcgtgaatt atttgaccga tttaagaatg aaattctcga tcgctattat tattcgcctg
     4261 accgagataa taaataccac gaaggttttt tgcgcggctt attgggtgaa attcgtgact
     4321 tgatgtataa agagccgccg gaaaatttac tcaatcccaa acgcgacggc ttgcggatgt
     4381 tgaaagctct aatttttgca ctgaagactt ggaagcgggt tgagggcaac acctcgctgg
     4441 aagttttgga cattttatta gtcaaagaac ggttcaatca tgaaaattat tatcaggttt
     4501 ttcaggcact aacttttctg gaaactttcc gctttctgta tcaattgttc gtcgtacagg
     4561 aggaagaaat tcatttagac agcgatgaaa tgcaacttag cctgcaaaaa gtagcctatg
     4621 cgatgggtta ccaagatcat agttacgctt tagcctgtac tcaaatgttg actcactatt
     4681 atgatcatca taaaatcgcc cggttaggaa ctgagcgtct agtagaaggg ctgaccgaac
     4741 atcttcgcca gattacgatc ttctacccga tcacgcattt ccaatcccgc gccaaaagca
     4801 aagcgcccct gccggataat attccacttt attttatgca gaccgcccgc ttttttcaag
     4861 gcgtacgttt ctgggatgat ttactctcca gtctcgataa tcccaaccaa attttgctga
     4921 aacgctttct ggcagatttt atgaaattat cggaagcaag acgggctgtc ctgatgaaag
     4981 cttatgtcaa gtggggtgta aaaactcctt atactatgat ttcgctgttc agcattatcg
     5041 ctgaccatca accgaaaata gtcgaaacgc agtttttcca ggaattcatt cgcgatttta
     5101 tttataatct ggaaccgact ctggaaaatg tcagccgact ttgtcaaatt ctgaatttag
     5161 ctccgcagat gatgaataac ttcctgtcaa ttttaccggt cgagctcttg acgcggttga
     5221 tagcagttgt cgaagaacct tactggaaac cagaaattac ctctctgcgc gatacactat
     5281 taatgctttg tcggatttat cgcagtagta gttattattt caagcgtttt gtacagcggg
     5341 cgttcaatac ttatgctgaa tacatcactt cgttaaccaa tcaacctaag ctcaatcaat
     5401 tggctgaagg tcttttccgc aacattgata atttcgattc gctcaatgaa aaaattacgc
     5461 ggttgggtga ttattacgat ttcgagttta tgcgtttggg aattgcttta ttagacggtg
     5521 cgcccttcga taaaattaat cgggaattca cgaatttttc ggataattat cttcagatgc
     5581 tttttgaact ctgtcaggag gaagtagcgg cggctgatca gaaagagatc ccggaaatgc
     5641 atgatctttt ggctatcttt acagctggcg ggcatgcccg cggtcaggct tttgacgatg
     5701 attatgacct aattgttttg ttgaattctg atagtccggc tattttagac ttcggtaata
     5761 aagtcataca aaaaatgaat aaatacatcc tgcgacgttc catcattccc caattccgct
     5821 ttgctgaccg tttccggcga tttgtgacca ctttcagcga tctgcgtaga ttattcgagc
     5881 aacccgatga aaatactttc attgatcaat cgcaattgct gggtgcgcgt ctggtaattg
     5941 gaagtgatta ttttttgaaa gtatttcaga gtgaaattat tactccattc attttccagc
     6001 gtaaggctga atttatcgaa acgatgttga atgaaatgag atcccgccac aattattata
     6061 atagtactaa aattgaagtt ccggaaatca aggaaggtcc aggcggcttg cgcgatttcg
     6121 agaattttat ctttattcta aaagcctact tcgaatgggc tgaacctttg accgaaacgt
     6181 tgttttcgcg gattgaatcg gcaataacca atgacgacag taacttcata gctctggtgc
     6241 gcgattatca gttactgaaa catattcgcg atttatatcg cctgtcagtt tctaacgatg
     6301 atcacctgga taaggcttac ttggatcagc tggttcgacc gttttgcctt gcccatggtc
     6361 tggaactaga aaatggcgct gaattggcgg agttcattca aaggattatg cgaaaaaacg
     6421 tagagagaat caacatttta ctgcggacat taggattgcc ggaaattggt gcattaccag
     6481 ggaaataatt cgccactttg aaaaatttga gttcagaact atttagcagt tcatagtatc
     6541 ttttttagaa cagttaacgc tgattcaatt gatcgccaga tttttgagtt tggttttcaa
     6601 tttctggtaa ggttttctac tttagaaaaa tatttgatga ttgatatttt ccttgtcgca
     6661 aaagtcttga attgcccgac tgactcgcca ccagtaatca ctgcgaccga acaagatttt
     6721 ttcgtaactg cttagcagtt cgggatgttt ggcaattaac cagcttttta tatgactcca
     6781 gattcgccca gatggattgg tatggtcgaa catataataa tcaacaaatt cgcgggtctg
     6841 aagaacaatc tgtttccagc cggtcagata aggtaaaatc ggtccgacga aaacataggt
     6901 ttctaatccg gcggctttga gtcgtcttaa ggcgtcaata cggtcagcga tgggcgatgc
     6961 gccgggctca atctcacgac gtaaattttc atcagtcgtc acgatcgtca tccccaccga
     7021 acaattttta aattgcgtca ataaatcaag gtctcgtata accagagccg atttggtctg
     7081 aacggtaagt tgcggctggg ctaatgtcaa ttgctttaga attcggcggg ttaattgata
     7141 ttcggcttcg accggttgat aaggatcgca gactgaggat agataaacgg tctggttgtg
     7201 gcatttcgcc aattgcctag tcaatatctc gagcgcattg ctttttacgt caacgtaagt
     7261 tccccatggc tcgctgtgcc cgtcgaactt ctgcataaaa caggcataac aatagacgca
     7321 ggcaagctga cagccgcgat agggattgat gacgaaatcc acccctggca atttggattg
     7381 gctgagaata ctgcgcacct taatttcttg aatattcgac ataactctta ctttactgaa
     7441 aaattaataa ataagtcaag tatggcatac tacaattaca gatatgcggt aaatcacatt
     7501 ttttaaaata ccataaaata ccatttgatt attgctattt ttccccctaa gcttacacaa
     7561 gagatatttt gagttgatta ttaatttaaa atggaggaca taacatgaaa acatctcaca
     7621 cgagagcttt tctctgcatt ttaatcgcgt ttttaatggt gactgtttct ctgacagccg
     7681 ccggtaacat tgcgacttat aacggcaaag aaattcgctc aaaccgaatc gtggtggtca
     7741 ataattcaat tttgaaaaaa ttgcccgcca aacctgatca gaccatgcgg ctcgaaagcg
     7801 cccgccaagc ttatcaggga cgtatcgtcc gaaacctgcc cacggaagga attgccgagt
     7861 gggagattaa aggcgatatg cggaaaactc tcgatgcctt gaatgccatt gacggcatca
     7921 gcgcttttcc gaattttgtt tttaaacggg agaatttgca acctaaacct attcaaaaag
     7981 atgctcttgt cccgaacgat tacttcattt ccctgcaatg ggcgctgaac aacgatggaa
     8041 catttgattc gttatatacc agtatgggag gtatgccagt tg
//
LOCUS       DAOSMP010000070         7974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_9047_length_7974_cov_20.384645, whole genome shotgun sequence.
ACCESSION   DAOSMP010000070 DAOSMP010000000
VERSION     DAOSMP010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9047_length_7974_cov_20.384645"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..1127)
                     /locus_tag="PKX09_09020"
     CDS             complement(198..1127)
                     /locus_tag="PKX09_09020"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOU18001.1"
                     /translation="MDLTPESPIPLINNYKKGVFPADCLVFGFTVLMLLIGITNYSRL
                     GKPYTWISTDCGIIIVLLMLIFKETHEERRFFYWLHFFWPIISLAFLYTQCTSWDNLI
                     FPITFDPVLIKWDLRLFGSALNKVMASSVDCLWLDEIMHFFYFSYYLVIFLTVVWMLG
                     RRQPQAYELIFSLTLMMYFHFIFFMIFPSDGPLADRNDLFDQGVVFIPLMDFIYGVSE
                     QNGGGAFPSTHVSAAVVVAIYTFKYSPKLRIPIGGLCLGIIISTVYCSYHYAIDVAAG
                     IITGTLFYFLGKYIYNHRCQPVNLALFMPQKSI"
     gene            complement(1118..2104)
                     /locus_tag="PKX09_09025"
     CDS             complement(1118..2104)
                     /locus_tag="PKX09_09025"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU18002.1"
                     /translation="MKVLVTGATGFIGQHLIRRLLAENCEVRALCRTTSLKPDEFAQS
                     VEWQIGDLTAPESLASAVSDCDLIFHLAGAIKALNDAAFTRINVNGTVNLLKALKQYG
                     KKEVRFIFVSSLSASGPSAPWSPKNESEPCYPVSAYGQSKLEAEIEVLKQKNRIWCTI
                     IRPAVVYGPGDKESLAFFKIAKSRFNPHLGFEKRYVGMIFVGDLVDLLWKAALSHRPS
                     GEIYFACDEQSDGYEWNQIIATAGKVLGYHTLPILVPQFILPMIVVPTQIIAKMTGKI
                     GSLNRDKYREMNQSHWTCTSAKAQKLLDFKPQVDLSEGFKRTAQWYREQNWI"
     gene            complement(2104..2943)
                     /locus_tag="PKX09_09030"
     CDS             complement(2104..2943)
                     /locus_tag="PKX09_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOU18003.1"
                     /translation="MLKPLAIESWPDMFFYRPLAFGLVKTIIPLPITPNMISLSAIII
                     GLSGGYCLAIGTPRSCQIAGLLFMANVILDCADGMLARYKHNGTPLGFIVDGLVDYCN
                     GAAIFIGMTVGFSRTDSQFGFSLWVLIPLSLFSWIAHSMAVDHARREFQRNALEQYPS
                     SAGGRQNFQNYREQLSRSKGHWSDKLLASLYLFYCNVQKPNAKFSRTYPADRYYRYNN
                     CVMRGWLLIDPSTHMSIAILSALLYNPLIFFWYTIVIANIIMLMMYLIQYFVNQKVGV
                     LPV"
     gene            complement(3038..3760)
                     /locus_tag="PKX09_09035"
     CDS             complement(3038..3760)
                     /locus_tag="PKX09_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18004.1"
                     /translation="MDNIFDIIILNGRPASGKSEVIDYLKKTPVEIRRRRFHIGELDE
                     IDDFPMLWTWFEEDAILEKILNKPRLHSDKDGYFFWEYLWHLLIERISMEYSKRLRDK
                     PNFHNNYTALIEFSRGSEHGGYRAAYPHLSDEILKWAAIFYIDVSFEESLRKNRQRFN
                     PAKPDSILEHGLPDAKLTRLYKDCDWEEFRGDDPEFIKIKGHKVPYVVLHNEPDITTA
                     RDENLGRVLEELLNKLWILRQR"
     gene            complement(3779..5158)
                     /locus_tag="PKX09_09040"
     CDS             complement(3779..5158)
                     /locus_tag="PKX09_09040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU18005.1"
                     /translation="MNTSETENLLAMPTFRHRRLQNWVIIGLLYSFFYMSRYNFSAIA
                     PTLQAFFGWTKSNLGVFETLMPLVYGLAVVINGPLADKIGGRKAFLIGALGVVIMNGL
                     FGFFHILVQTPAVWVDQEIVSEAVLKFGFSKSALLWTLAIIWAINGYFQSFGALSIVK
                     INAQWFHIRERGTFSAFFGVLIRFGLILAFSGTPLIVKFLPWYWAFWIPALVVALLFV
                     LTIFFVRDTPAEAGFPELDTGDGDVNLEEKVKFKEVLTKVFGSKVMWTIAIGSMMIGF
                     VRRGVVDAWWPIYFKEMQGVTGVDFAFQLTAWGIAILGIIGGFAFGISSDRVFGGRRA
                     PVIVMGFCGMIVILTLFFLADVWQWGPIAAAILLACLSFFVNGSHGMIAGAATMDFGG
                     RKAAATAVGLIDGMQYIAGAFVGALVGWVTTNWGWQAWKIWPIPFAILGALVMSSIWH
                     TTPTGKSTH"
     gene            5289..6938
                     /locus_tag="PKX09_09045"
     CDS             5289..6938
                     /locus_tag="PKX09_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOU18006.1"
                     /translation="MIEVNLGEKRTITVHPGQKIIEIVKQALPHNRYKPYIVIRNNTF
                     TSFNEIVNEPTKISLLRYFHESSRRAYESAAILILNYVVEDLYPAQRLIVMHSMCDGV
                     FCRFVNEDFCTPQIAQEISAAFIKVVEADYAIEPLLISRNDARRYFKENGQKDTEELL
                     KYSEANFVTLYQINGRRFWLLSPPAPCTGLIKVFEIRPYQKGFVLRCPTEGADRRLRP
                     FTDQARLYEIFQETEEWGRILEISTVADLNRTIVENQISDLIKIDEALHEKKIASIAD
                     QISNKGEHRFVFVAGPSSSGKTTFAKRLAIQLRVLGFKPLLLSLDNYFRDRAELAKLQ
                     GKRINYEVLDAIDLELLNDHLQRLLRGETITPPVYNFITGTKTPGTKPMQINSQTMLI
                     FEGIHGINPGLTPEVNERYKFKIYVSALTHLNFDHMNRVRTHDTRLLRRIVRDYKYRG
                     YSAAETIEMWKKVVEGENKYIFTFQGNADIMFNSSLVYEHGVLKTLAVTMLKNVPESH
                     HSYPEAQRLLDILSYFLPVDDAEIPPTSIMREFIGKSSFTY"
     gene            complement(7014..7433)
                     /locus_tag="PKX09_09050"
     CDS             complement(7014..7433)
                     /locus_tag="PKX09_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOU18007.1"
                     /translation="MALSKGSVNKVILVGRLGRDPEVRYTPNGDAVAEVSLATTESVK
                     NRDGKREDRTEWHNLVIWRNQAEFAKEWLKKGQLVYVEGRLQTRQWEDKEGQKRSKTD
                     IQVDQITMLGGSGRGREKEAAEPETPPEPSPDDEIPF"
     gene            complement(7535..>7974)
                     /locus_tag="PKX09_09055"
     CDS             complement(7535..>7974)
                     /locus_tag="PKX09_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU18008.1"
                     /translation="CISGRREIVEFMRQRSRPYLFSNSVAPAVIGATLKALEIVRENP
                     DLPRRTIENAHLFRQKIEAAGFAIIPGNTAIIPIMIYDEPLAIKIADLLLEEGIYVIG
                     FTYPVVPRGTARIRVQVSAAHTPEQIDRAVAAFTKVRKSLNKG"
BASE COUNT         2348 a         1811 c         1748 g         2067 t
ORIGIN      
        1 tatcgatggt ctgtacggtg aaagcgatcc attgcccgtc gggtgaatac tggggctccg
       61 ccagtcggtg acaatttagt aagtcatcaa tctgcatagc gcgcttagca gtgaatccta
      121 tatccaccat tatgattaga gatagccata ttaatccgac agcttttgac ttcattggta
      181 actcccttat ataggtttta gatgcttttt tggggcataa atagcgctaa attgacgggc
      241 tgacagcggt ggttatagat atatttaccg agaaaataaa aaagtgtgcc ggtgataatt
      301 ccggcagcca catctatggc ataatggtaa ctgcaatata cggtggagat tataattccg
      361 agacacaaac ccccgatcgg aatgcgcagc ttgggtgaat atttaaaagt gtaaatggca
      421 accacaaccg cggcggaaac atgcgtgctg ggaaaagcac cgccaccatt ttgttcggac
      481 actccataaa taaagtccat tagggggata aaaaccacac cctgatcaaa gagatcgtta
      541 cggtccgcca gcggtccatc gcttgggaaa atcataaaga atataaaatg gaaatacatc
      601 ataagcgtca gactgaaaat caattcataa gcctgaggct gacgccgccc aagcatccaa
      661 acaactgtta aaaagatgac taagtaatat gaaaaataaa agaaatgcat tatttcatcg
      721 agccacagac aatcgaccga tgaagccatt actttgttca aagccgaacc gaacagcctg
      781 agatcccatt ttataagtac gggatcgaaa gtaatgggga aaatgagatt atcccaggag
      841 gtacattgcg tatatagaaa tgccagggat ataatcggcc agaaaaaatg aagccagtaa
      901 aaaaatcgcc gctcttcgtg cgtttccttg aaaatcaaca ttaatagcac aataattatt
      961 ccacagtcgg tactaatcca ggtatatggc ttgccaagcc gagaataatt ggtaattccg
     1021 attaatagca ttaacacagt aaaaccgaat accaggcaat cggctggaaa tactcccttt
     1081 ttgtagttgt tgattaaggg aatgggtgat tcgggtgtta gatccaattt tgctctcggt
     1141 accattgagc tgtgcgttta aaaccttctg aaagatcaac ctgaggctta aaatcaagca
     1201 atttttgggc tttggcggat gtacaggtcc aatgggattg attcatttcg cgatatttgt
     1261 cgcgattaag actaccaatt tttccagtca tttttgcaat aatctgggtg ggaactacga
     1321 tcatcggtaa gataaattgc ggcactaaaa ttggtaatgt atgatagccc agaactttac
     1381 cagccgtagc aataatctga ttccactcat agccgtcgga ttgttcatcg caggcgaaat
     1441 atatttcgcc ggatggacgg tggctcaaag ccgccttcca taacaaatcc actagatcgc
     1501 caacaaagat cataccaacg taacgttttt cgaaaccgag atgcggatta aaacgcgatt
     1561 tagcgatttt gaagaaagcc agtgattctt tatcgccggg tccataaacc accgccggac
     1621 gaataattgt gcaccagatt ctgtttttct gctttagaac ttcgatttcg gcttcgagtt
     1681 tgctctgtcc gtaagctgaa actggataac agggctcaga ctcatttttc ggtgaccaag
     1741 gcgcagacgg cccactggcg gaaagtgaac tgacgaaaat gaaccgcacc tctttcttgc
     1801 cgtattgctt caaggctttc aaaagattga cagtcccatt gacgttgatc cgggtgaaag
     1861 cggcatcatt tagggctttg atagcaccgg caagatgaaa aattaggtca caatctgaga
     1921 cggctgaagc cagcgattct ggagcggtaa ggtcacctat ttgccactcg acactttgcg
     1981 caaattcatc cggtttgaga ctggtggtac ggcaaagagc ccgcacctcg caattttctg
     2041 ctagtaaacg tcggataaga tgctgaccaa taaagccggt tgcaccggtt acaagaactt
     2101 tcatcagacc ggcaagactc ccactttttg gtttacaaaa tactgaatca gatacatcat
     2161 taacataatg atattagcaa ttacgattgt gtaccagaaa aatatcaacg gattatataa
     2221 taatgccgac agaattgcaa tggacatatg ggtagaagga tcgatcaata accaaccgcg
     2281 cataacacaa ttgttgtagc gataatatct gtcagccggg taggtgcggc taaatttggc
     2341 attcggtttc tggacattgc aatagaatag gtaaaggcta gccagcaatt tatcagacca
     2401 gtgacccttc gagcggctta gctgttcacg ataattctga aaattttgcc gacctccggc
     2461 tgaggacgga tactgttcga gagcattgcg ctgaaattcc cgccgggcgt gatcgactgc
     2521 catcgaatga gcgatccacg aaaagagact caatggaata agcacccaga gcgagaatcc
     2581 aaattgtgaa tcagtacgac tgaaaccgac ggtcatacct atgaaaatcg cggctccgtt
     2641 gcaatagtca accagaccat caactataaa gcccagcggc gttccattgt gtttataacg
     2701 cgccaacata ccgtccgcgc aatccagaat gacattggcc ataaacagca aaccggcgat
     2761 ttggcagctc cgcggagtgc cgatcgctag acagtagccg ccgcttaaac cgatgataat
     2821 cgccgaaagc gaaatcatat tcggcgtaat cggcaagggt ataatggtct tcaccaaacc
     2881 gaaagccagc ggtcgataga aaaacatatc cggccaactt tcgatcgcga gcggtttcag
     2941 cattgaacga tattctctgt acaacttatg ccagattttc ccaggcgatt tggtcataaa
     3001 ttcattttac cgatataatc cgatataatc caggatttta tctctgtcgt agaatccaga
     3061 gtttatttaa tagttcttct aatacacgac cgagattttc gtcacgggca gtcgtgatat
     3121 ccggttcgtt atgtagaacg acataaggca ctttatgccc tttaatttta ataaattccg
     3181 ggtcatcacc gcggaattct tcccaatcgc aatctttata gagacgggtt aactttgcat
     3241 ccggcagtcc atgttctaaa atgctatccg gtttagcggg attaaagcgc tggcgatttt
     3301 tacgcagtga ttcttcgaac gatacgtcaa tgtagaaaat agccgcccat tttaaaattt
     3361 catcagataa atgcgggtaa gccgctcgat aaccgccgtg ttcactaccg cgtgaaaatt
     3421 caataagagc cgtgtagtta ttatgaaaat tgggtttgtc gcgcagacgt ttactgtatt
     3481 ccattgagat acgctcaatg agtaaatgcc aaagatattc ccagaaaaaa taaccatctt
     3541 tgtcggaatg cagtcgcggt ttattcaata ttttttccag aatggcgtct tcttcgaacc
     3601 aggtccagag catcggaaaa tcatcaattt catccaactc gccaatatga aaacggcgcc
     3661 ggcgtatttc caccggagtt tttttgagat aatcaatgac ctcggacttg ccggatgctg
     3721 gtcgtccatt aagaatgata atgtcgaaaa tattatccat aaaaatgctc cttgattatt
     3781 aatgggttga ttttccagtg ggagtagtat gccagataga agacattacc agtgcgccca
     3841 gaatggcaaa gggaattggc cagattttcc aggcttgcca gccccaattg gtagtaaccc
     3901 agccgactag cgcgccaacg aatgctcccg cgatatactg cataccgtca attaatccga
     3961 cggcggtcgc ggcggctttg cgaccaccaa agtccatcgt agccgctccg gcaatcatac
     4021 cgtgggaacc gttaacgaaa aatgacaagc aagctaacaa aatagccgcg gcaatcggtc
     4081 cccattgcca tacgtcagct aaaaagaata gggtgagaat tacaatcata ccgcagaaac
     4141 ccattacgat cacgggtgca cgccggccgc caaagacccg atcggaggaa attccaaagg
     4201 caaaaccgcc gattattccc agaatagcaa tcccccaggc ggttaattga aaagcgaaat
     4261 ctactccggt aaccccctgc atttctttga aataaattgg ccaccaggca tctaccacac
     4321 cgcgccgtac aaaacctatc atcatcgaac caatggcaat agtccacata accttactgc
     4381 cgaacacctt ggtaagcact tctttgaact tgactttttc ctcaagattg acgtcaccat
     4441 cgcccgtatc gagttccgga aagccagctt cggcgggagt gtctcgtaca aagaaaatgg
     4501 tcaggacgaa aagtagtgct actactaatg ccggaatcca aaaagcccaa taccatggca
     4561 gaaattttac gatcaatggt gtcccggaaa aagccagtat cagaccaaat cggattagca
     4621 cgccgaaaaa tgcagagaat gtgccccgct ctctgatatg gaaccattgg gcattgattt
     4681 ttacgattga taatgcgccg aaagattgaa aatagccatt aattgcccag ataatagcta
     4741 gggtccagag aagcgccgat ttggagaatc caaattttaa aacggcttcg gagacaatct
     4801 cttgatcaac ccagacggca ggagtttgga ccaaaatgtg gaaaaatcca aacagcccgt
     4861 tcataattac aacgcccagg gcgccaatca ggaaagcctt gcgaccaccg attttatcgg
     4921 cgagcgggcc attgatgacg accgccaaac cataaaccaa gggcataagt gtttcgaaaa
     4981 cccctaaatt gcttttagtc cagccgaaaa aagcttggag agtcggcgca atagctgaaa
     5041 aattataacg ggacatatag aaaaaggagt acaacaagcc gatgataacc cagttttgaa
     5101 gacgtctgtg tcgaaaagtc ggcatagcca gcaaattttc agtttccgaa gtattcattt
     5161 tgatctcccc attttatttt ttagcgagta atgaacaatc atttaccatt aacttgaaat
     5221 tattaatcta tcgtcaaaaa ccaaacttta tttttcgata ttttagtgat aatttccagt
     5281 ttatggaaat gatagaagtg aatcttgggg aaaagcgaac gattactgtc catcccggcc
     5341 aaaaaataat tgaaattgtt aaacaagctc tgccgcataa ccgatataaa ccgtatatcg
     5401 ttattcgcaa caatactttt acgtcattta acgaaattgt caacgagccg actaaaatca
     5461 gccttctgcg atacttccat gagtcatccc ggcgagctta tgaaagcgca gctatcctga
     5521 ttttaaatta tgtggtcgag gatttgtatc ctgctcagcg tctgatcgtg atgcatagta
     5581 tgtgcgacgg cgtcttttgc cgattcgtga atgaagattt ttgcacgcct caaatcgctc
     5641 aagaaatttc agcggctttt atcaaagtgg tagaagccga ttatgcgatc gaaccgcttt
     5701 taatttcccg taacgacgcc cgtcggtatt ttaaagaaaa cgggcaaaaa gatactgaag
     5761 aattgctgaa atatagtgaa gccaattttg ttacattata ccaaattaac ggtcgccgtt
     5821 tctggttact ctcaccgccg gctccctgta ccggactgat taaagtattc gaaattagac
     5881 cctaccaaaa aggattcgtc ctacgttgtc cgactgaagg agctgatcgt cgtctgcgac
     5941 ctttcacgga tcaggcgcga ctgtatgaaa tttttcagga gactgaggaa tggggacgca
     6001 ttttagaaat cagtacggta gccgatctca atcgtacgat agtcgaaaat cagatttcgg
     6061 atttaatcaa aattgatgaa gcattacacg agaaaaaaat agccagcatt gctgatcaga
     6121 tttcaaataa aggcgagcat cgttttgtat ttgtagctgg gccatcgtct tccggcaaga
     6181 ctacctttgc caagcgcctc gctattcagt tacgggttct gggattcaaa ccccttctgt
     6241 tgtcactgga caattacttc cgtgaccggg ctgaactggc caaacttcag ggcaagcgaa
     6301 ttaattatga agtgctggat gcgattgacc tcgagctctt gaacgatcat ctgcaacgac
     6361 ttttacgggg cgagacgatt accccgccgg tatataattt tattactggg accaaaactc
     6421 ccggcacaaa acccatgcaa attaattccc aaacgatgtt aatttttgaa ggaattcacg
     6481 gcattaatcc gggcttgact cctgaagtta atgaacgtta taaattcaaa atttacgtta
     6541 gtgcccttac tcacttgaat tttgatcata tgaaccgtgt tcgtactcat gacacccgac
     6601 tattacgccg gattgtacgc gattataaat atcgcgggta tagtgcggct gaaactatcg
     6661 aaatgtggaa gaaagttgtc gagggtgaga ataaatatat tttcaccttc cagggcaacg
     6721 ccgatatcat gttcaattca tcgctggtct atgaacatgg cgtcttgaaa accttggcgg
     6781 ttaccatgct gaaaaatgtg ccggaaagtc atcacagcta tccggaagca caacgcttgc
     6841 tggatatact ttcttatttt ttaccggtgg atgatgctga aattccgccg acctcgatta
     6901 tgcgggaatt catcggtaaa agttccttta cctattaatg cgttgagatc gaccggatcg
     6961 gcgccaaacg gcgtcagatt tatcctaaga ttacaatgga ttataagata atcttagaag
     7021 ggaatttcat catccggaga aggctcgggc ggcgtttccg gttcggcggc ttctttttcg
     7081 cgtccgcgtc cggaaccacc cagcatagta atttggtcta cctgaatgtc agttttacta
     7141 cgtttttgac cttctttatc ttcccattga cgggtttgga gacgaccttc gacgtagacc
     7201 agctgtccct ttttgagcca ttccttggca aattctgcct gattgcgcca aatcactaga
     7261 ttgtgccact cggtgcggtc ttcacgtttt ccgtcacggt tttttaccga ttcagttgtt
     7321 gctaaggaaa cctcggcaac tgcatcgccg ttcggggtgt agcggacttc cggatcgcgg
     7381 ccaagacgac caaccagtat gaccttgtta acacttccct ttgaaagagc catcgttaat
     7441 cctccttttt gttaaaaatt aaatcctcaa aattgggatt gcaacttccg atgctaattc
     7501 tacaaaaaac ttataacttt gtcaaacact atttttatcc cttattgaga gatttcctta
     7561 ctttcgtaaa agccgccacg gctcgatcaa tctgctcggg agtgtgagcc gccgaaacct
     7621 gaacgcgaat gcgggcggtg ccgcgcggta ccaccggata ggtgaaccca ataacataaa
     7681 tgccttcctc aagcaaaaga tcggcgattt taatggcgag aggttcatcg taaatcataa
     7741 tcgggattat ggcggtattg ccggggataa tggcgaaacc ggcggcttcg attttctggc
     7801 gaaaaaggtg tgcgttttca atcgtgcggc gtggcaagtc cggattttct cgcacaattt
     7861 ccaaagcctt taatgtggct ccgataacgg ccggcgccac ggaattcgaa aaaaggtaag
     7921 gacgcgaacg ctgacgcata aattccacaa tttcccgccg cccggaaatg caac
//
LOCUS       DAOSMP010000071         7726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_9432_length_7726_cov_16.825577, whole genome shotgun sequence.
ACCESSION   DAOSMP010000071 DAOSMP010000000
VERSION     DAOSMP010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9432_length_7726_cov_16.825577"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    77..176
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(804..1997)
                     /locus_tag="PKX09_09060"
     CDS             complement(804..1997)
                     /locus_tag="PKX09_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005545317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOU18009.1"
                     /translation="MKRYIGTVSRGIRMPIIRQGDAIEEIVVANVLQAAEAEKFSIRD
                     RDIVGITESVVARAQGNYATADAIATDLKAKFNGDCLGVVFPILSRNRYAIILKGIAR
                     SAKHIVLQFSYPCDEVGNQLMDPDLIDKNNVNPWSDNYTEAQFQRLFGQCCHPFTGID
                     YIAYYRSLVEGEKAECTIIFSNQPKTILSYTKKVLACDVHTRERTKRQLIAAGAEIVY
                     SLAEILSAPVNGSGYNPEYGLLGSNKATEEIVKLFPRNCQPVVDRVQQILKDKTGKTV
                     EVLIYGDGAFKDPVGKIWELADPVVSPAYTAGLKGTPNELKLKYLADNQFAGLTGQAL
                     EEAIAKQIRNKKENLVGSMESQGTTPRRLTDLIGSLCDLTSGSGDKGTPVVHIQGYFD
                     SFASE"
     gene            2429..3412
                     /locus_tag="PKX09_09065"
     CDS             2429..3412
                     /locus_tag="PKX09_09065"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOU18010.1"
                     /translation="MQYRELGRTGWKVSAISFGTWAIGGTWGTVRDQESLAALHRALD
                     LGVNFFDTADVYGDGHSERLLARLRHERSEPFYIATKAGRRLTPHIAGGYNRENLTGF
                     VERSLKNLETETIDLLQLHCPPTEVYYMPEVFDILNDLVKAGKIRYYGVSVEKVEEAL
                     KAIEFPGVQSVQIIFNMLRQRPADLFFKEAKRKQVGILARVPLASGLLTGKFSPSSTF
                     ESDDHRSFNRHGEAFDRGETFAGLEFNAGLQVIEKLRLLVPEGFTMTQMALRWILMFP
                     EVTCAIPGAKRPEQVAENAQAADLPPLSDAIMQKIQEIYERDVRPHVHQYW"
     gene            3697..3984
                     /locus_tag="PKX09_09070"
     CDS             3697..3984
                     /locus_tag="PKX09_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOU18011.1"
                     /translation="MAYMYILKCSDGSYYTGSTVDLERRLSQHQNGEGANYTKKRLPA
                     ELVYFEEYSSIADAFYREKQVQGWSKKKKEALINGEYEDLKHLAKKVWKKK"
     gene            complement(4263..4655)
                     /locus_tag="PKX09_09075"
     CDS             complement(4263..4655)
                     /locus_tag="PKX09_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOU18012.1"
                     /translation="MVIKQISVFIENKKGRLADVTNVLANENINIKALSLADTSDFGI
                     LRMIVDEIEKAIYSLKQAGFIVKETDVLAVEVEDKPGGLNSILQKFKEYNIDIAYMYA
                     FVESLNSNALLVFKVNDIEKALKVLNQE"
     gene            complement(4666..5967)
                     /locus_tag="PKX09_09080"
     CDS             complement(4666..5967)
                     /locus_tag="PKX09_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOU18013.1"
                     /translation="MIWNTEIETADRAKLNNIQSERLLTLIRRVYNNNDFYREKFKKA
                     RVKPSDIKSIEDIVKLPFTSKVEMINGYPYGLLSVDKKYVVEIHSSSGTTAKPVSSCY
                     TANDIELWSEVMARSLTACGVTANDTIQNAYGYGLFTGGFGFHYGARKIGANLIPVSG
                     GNTKRQLMIMQDFGTDILTCTPSYSLHIADVAKEMGIDFSQLQLRAGVFGAEPWTESM
                     RNEIQSKLSIKAYDIYGLTEIIGPGVAIECEYLNGLHIFEDVFYPEVIDPKTGELLPP
                     GSQGELVLTTLTREATPVIRYRTRDIVTVNYDKCKCGRTHVKMSKVVGRTDDMLIIRG
                     VNVFPSQIEEVLLKFEETEPHYQLIVNRKLNLDCLEVYVEMTEKLFSDEIKEIQESER
                     KIENELYNVLNLKAKVTLVQPGYIKRTDGKAQRIIDKRNFN"
     gene            6614..>7726
                     /locus_tag="PKX09_09085"
     rRNA            6614..>7726
                     /locus_tag="PKX09_09085"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         2135 a         1634 c         1620 g         2237 t
ORIGIN      
        1 tgcacttgat ttaagccgtc acccttcgac aagctcaggg tgacatttct ggtttcctgc
       61 ttctatttct ctcattnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctcc
      181 ctccttcatc cttagccttg aagaaagtct aagctcaggg tgacatttct ggtttcctgc
      241 ttctatttct ctcattctcc ctccttcatc cttagccttg aagaaagtct aagctcaggg
      301 tgacatttct gatcttttac ttctatttct tgttttctcc tctttactca gcctaaaaga
      361 aaggtcaagg cagagaaaaa aggctaagat ttgaattttt ttctcaacct caatcttttt
      421 actcagcctc tttaccaatg accaatgacc aatgcccaat taccaatgac caatgaccaa
      481 tgcccaatta ccaatgacca atgactagtg accaattacc aatgaccaat gaccaatgac
      541 taatgaccaa tgacctttct ctgccttatt tttttgtttt gggtcttgga agaataaacc
      601 gccaagttcg ccaagaaccg ctaaatgagt aaaatcccaa accccaaatt ccaaatcaca
      661 aatagctaaa ccaatttccg caaaactgtc tcctgtctcc ttttctccat ttctcagtct
      721 cttttctctt tactcagcct ctttactaat gaccaatgac caatgaccaa ttaccaatgc
      781 ccaatgccca atgccccagt tatttattcg ctggcgaagc tatcgaaata tccttgaata
      841 tgaacaaccg gtgtgccttt atcgccgctt ccggaggtca gatcgcataa cgaaccaatc
      901 agatcggtca accggcgcgg cgttgttccc tgggattcca tagagccaac cagattctct
      961 tttttattcc gaatttgttt agcaatcgct tcttctaaag cctgaccagt taggccggca
     1021 aattgattat cggcgagata tttcagcttt aattcattgg gcgtaccttt caaacctgca
     1081 gtataagccg gagagactac gggatctgct aattcccata tttttccaac cgggtctttg
     1141 aaagcaccat cgccgtagat caatacttcc actgtctttc cggttttatc ttttaagatt
     1201 tgctgaacgc gatctaccac cggttggcaa ttacggggaa ataacttcac aatttcttcg
     1261 gtggctttgt ttgaacccag cagtccgtat tccggattat agccactacc gttcacggga
     1321 gctgataaaa tttcagccag gctatagaca atttccgcac cggcggcaat taactgtcgc
     1381 ttggttcgct ctcgggtatg aacatcacaa gccagaacct ttttagtgta tgacagaatc
     1441 gttttgggct ggttggaaaa aattatcgtg cattcagcct tttctccttc taccaatgac
     1501 cgataataag caatataatc tattccagta aaaggatggc agcattgacc gaacaatcgt
     1561 tggaattgag cttcggtata attatcgctc cacggattaa cgttgttttt atcaattaag
     1621 tccggatcca tcaattgatt gccaacttca tcgcaaggat aactgaattg cagtacgata
     1681 tgtttggcac tgcgggcaat gcctttgagt ataattgcat agcggttacg actgaggatc
     1741 gggaatacaa ctcccagaca gtcgccattg aatttcgcct tcaaatcagt ggcgatggca
     1801 tctgccgtag cataattgcc ttgcgctcgc gcgacgaccg attccgtaat tcccacaata
     1861 tcccgatcgc ggatagaaaa tttctcggct tcggcggctt ggaggacgtt tgccactact
     1921 atttcttcaa tcgcatcacc ctggcggata atcggcattc gaattcctct agataccgtt
     1981 cctatataac gtttcattta atcggtctcc tcattaaatt ggcataaatt tattttttca
     2041 gtaaatgttt agaaaaggat tttccatatt gagggttggt ttatatcttt tttcgttcac
     2101 taaaaatttc gcaatttaat agtttattac tttatcaagg ctcattttta acagtgtgat
     2161 tttaggtaat ttttagttgt caaatcaata aaaaaataga aaaaaaatag tgaatccata
     2221 taaaatcgca agattacagg aatatcgaag taattttcgc ctcgtgactt ttagttcttg
     2281 ttcatcaaaa ttcacaaatt gaaccttaac gaccaaataa aatttcacta aattagcaga
     2341 tgattgatta attttaagaa aatggaaatt agtctgtaga ttgatttcag tttatttaca
     2401 aatcattcat taacatcagg agtagaaaat gcaatatcga gaattaggac gcaccggctg
     2461 gaaagtttca gccattagct ttggaacgtg ggcaattggc ggaacgtggg gcaccgtcag
     2521 ggatcaggaa tcactggcgg ctcttcaccg agcgctcgac cttggggtaa attttttcga
     2581 cacggccgat gtctatggcg atggacatag cgagcgcttg ctggcgcgcc tgcgtcacga
     2641 aagaagtgag ccgttttata ttgccacaaa agccggtcgg cgactcactc cgcatatagc
     2701 cggcggctat aatcgtgaaa atctcactgg tttcgtggaa cgcagtttga aaaatctcga
     2761 gacggaaacg attgaccttc tgcaattaca ctgtccaccg actgaagttt actatatgcc
     2821 ggaagtgttt gacatcctaa acgatctggt caaagccggc aaaattcgct actacggcgt
     2881 gagtgtggaa aaagtcgagg aggctctcaa ggcaatcgaa tttcccggcg ttcaaagtgt
     2941 gcagataatc ttcaatatgt tgcggcaaag accggccgat ttgttcttca aggaagccaa
     3001 gcgcaaacag gttgggattc tggcgcgcgt gccgctggcg tccggactgc tgactggcaa
     3061 attttcgccg agcagtacct ttgaatccga tgatcaccgc tctttcaatc gtcatggcga
     3121 agcctttgac cgcggcgaaa ctttcgccgg tttggaattc aatgccggat tgcaagttat
     3181 cgagaaactg cgcctgctgg ttccggaagg tttcactatg acgcaaatgg ctctgcgctg
     3241 gattctgatg ttcccggaag tcacctgcgc cattcccggc gccaaacgcc cggaacaagt
     3301 cgcagaaaat gctcaagcgg ctgacttacc gcctttatcc gacgctatta tgcagaaaat
     3361 ccaggaaatt tacgaacgcg atgtgcgccc ccatgtgcat caatactggt aaaaagttac
     3421 tataataaaa gcgcggaatg agtgacaagc aaaggttttt ccactagagt tatgtgatgt
     3481 aaagtaaaga tgaatggaaa aaaaatacaa gcggttgctt cggtacgccc tggcgggcta
     3541 ctcagcaacc gacgagagtt actcgtcaac cctgatgggc tactcagcaa ccgtccctat
     3601 tcggtggctg agtaaggcga aagcctgtat cgagcctggt ggctgagtag ggtgtaaacc
     3661 cgtaccgaag ccaccgtcac actaaactaa gtaactatgg catacatgta tatcctaaaa
     3721 tgttctgatg gcagctacta tactggtagt actgtggatc ttgaaagacg attatcccaa
     3781 caccaaaatg gcgaaggagc aaactatacg aaaaagagat tacctgcaga acttgtctat
     3841 tttgaggaat attccagcat agctgatgct ttttaccgag aaaaacaagt tcaaggttgg
     3901 tcaaaaaaga aaaaagaagc tcttattaat ggggaatatg aagatttgaa acacttagcc
     3961 aaaaaagttt ggaaaaagaa atgagtaaag tcgcagttga gcggcaataa ggggattggg
     4021 gcggttgctt cggtacgccc taacgggcta ctcagcaacc gttcccattc ggtggctgag
     4081 taaggtgtaa acccgtatcg agcctggtgg ctgagtaggg cgaaagcctg tatcgagcct
     4141 ggtggctgag tagggcgaaa gcctgtatcg aagccaccgc ccattttggc gatgaatacc
     4201 taaccaataa ttcgtttaat cttacatgat aattaaaaaa ataaaggatg tatctattac
     4261 gactattcct gattcaaaac tttaagagcc ttctcaatat cgtttacttt aaaaactaaa
     4321 agtgcgttac tgtttaaact ttccacaaag gcatacatat aggcgatatc tatattatac
     4381 tccttaaact tttgcagtat cgagtttaat cctcccggtt tatcctctac ttcaaccgcc
     4441 aaaacgtcgg tttcttttac gataaaacca gcttgtttta gggagtaaat cgccttttct
     4501 atttcatcca cgatcattct taatattcca aaatcactag tatcagcaag agaaagagct
     4561 tttatgttaa tgttttcatt tgctaaaaca tttgttacgt cggctagtct tcccttttta
     4621 ttttcgataa aaactgatat ttgtttaata accatttgat tactcctaat taaagtttct
     4681 tttatctatt attctttggg cttttccatc agttctttta atgtatcccg gctgtaccaa
     4741 agtgacttta gcttttaaat tgagcacgtt ataaagttcg ttctctattt tcctttcaga
     4801 ttcctgaatt tctttaatct catcggaaaa gagtttctcg gtcatttcta catatacttc
     4861 aagacaatca agatttaatt tcctatttac tataagctgg taatgtggtt cggtttcctc
     4921 aaatttaagc agtacctctt caatctgaga gggaaataca tttacgcccc taataatcaa
     4981 catatcatcg gttcggccta ctactttact cattttgaca tgagttcgtc cgcacttaca
     5041 tttatcataa tttacagtta ctatatctct ggttcggtat ctgataacag gcgtcgcttc
     5101 gcgcgtaagg gttgttaaaa ccagttcgcc ttgagagccg ggtggtaaga gctcgccggt
     5161 tttaggatcg attacctcgg gataaaatac atcctcaaaa atgtgtagtc cgttcagata
     5221 ttcacattcg attgccaccc ctggacctat tatttcggtt agtccataaa tatcataagc
     5281 ttttattgat aattttgatt ggatctcatt cctcatagat tcagtccaag gctctgctcc
     5341 gaaaactccc gcacgtaatt gaagttggga aaaatctatg cccatttcct tagccacatc
     5401 tgctatatgt agactatatg aaggggtgca ggttagtata tctgtcccga aatcttgcat
     5461 aatcattagt tgtcttttag tgttaccgcc tgaaacgggt attaagtttg ccccaatttt
     5521 ccgagcaccg taatggaatc caaaaccacc ggtgaataat ccatagccgt atgcattttg
     5581 aattgtgtca tttgcagtca cgccacaagc cgttaatgac cttgccatta cttcagacca
     5641 gagttcaata tcattggctg tgtaacaaga tgatactggt tttgcggttg ttcccgaaga
     5701 actatgtatc tctacgacgt attttttatc gacgcttaat aaaccgtagg gatacccgtt
     5761 tatcatttca actttagatg taaagggtaa ttttacaatg tcttctatgc tttttatatc
     5821 gcttggctta actctagctt ttttgaattt ttctcgataa aaatcattat tattataaac
     5881 tctacgaatt aatgttagta acctctctga ttgaatattg tttaattttg ctctatcagc
     5941 agtttcaatt tcagtattcc agatcataat aaattcccag ctttgttatt gttcacttaa
     6001 atcaaaaatt atttaatgtt agaaaattag acaagaaatt gtgataatga ttgatttttt
     6061 tatgattacc taattttatt ctccttttgg ggttcattat ccaactatta gatgttttag
     6121 cggggatatt aaggagaaaa atcgggtaag aaacaagcgg ttgcttcgat acaccctaac
     6181 gggttactca gcaaccgaat agagttactc gtcgatcctg gcgggctact ctactcagca
     6241 actgtcccta ttcggtggct gagtagggtg taaacccgta ccgatctcgg tggctgagtt
     6301 gggcgtaagc ccgtatcgaa gccaccgttt cgagctactc cgcaaatgat gatttgattt
     6361 catactcatc gaataaactt tgggtcattt ttggtggcaa ccgatgataa aaaattttaa
     6421 ttaaaatatt aaattttact tgcctaatat tttaatatgt tttatattca ccctgctaat
     6481 tgagaattga tttttaagtc cagtccgaag agggctgagg cttaaagtaa gaagctattt
     6541 gacaagagag aaagtgtgag atttgagaca ggtgtagggt aaaaaaaccc aaaaatttaa
     6601 tttgttgtat aatacaacgg agagtttgat cctggctcag gacgaacgct ggcggcgtgc
     6661 ctaacacatg caagtcaagg agaattcccg acttgtcggg atagtaaact ggcgaacggg
     6721 tgagtaacac gtaggtaatc taccctcggg atcgggataa cctcgcgaaa gtggggataa
     6781 taccgaataa agtctcggta taatttgtcg agatgaaaga tggggatctt cggacctatt
     6841 acctgaggat gagcctgcgc ctgattagct agttggtagg gtaaatgcct accaaggcaa
     6901 cgatcagtag ctggtctgag aggatgatca gccacattgg gactgagata cggcccagac
     6961 tcctacggga ggcagcagtg aggaattttg cgcaatgggc gaaagcctga cgcagcaacg
     7021 ccgcgtgact gattaagctc tgaggagtgt aaaagtctgt cgtagaggaa gaaactatcc
     7081 gataagaata ttgtcggata ctgacggtac tctatgagaa agctccggct aactccgtgc
     7141 cagcagccgc ggtaatacgg ggggagcaag cgttgtccgg aatcactggg cgtaaagagc
     7201 gcgtaggcgt cctaataagt ttctggtgaa atactcctgc tcaacgggag aattgctgga
     7261 gatactgtta ggattgagtt tagaagaggg aagcggaatt cctggtgtag cggtgaaatg
     7321 cgtagatatc aggaagaaca ccgatggcga aggcagcttc ctagtctaac actgacgctg
     7381 tggcgcgaaa gcgtggggag caaacaggat tagataccct ggtagtccac gccctaaacg
     7441 atggatactt ggcgttggga gctcgaccct tccagtgccg aagtaaacgc gttaagtatc
     7501 ccgcctgggg actacggccg caaggttgaa actcaaagga attgacgggg gcccgcacaa
     7561 gcggtggaac atgtggttta attcgatgca acgcgaagaa ccttacctgg gcttgacata
     7621 tactagaaag gtctgggaaa ccagatccct ttccgggttt actcggaaac tggtatacag
     7681 gtggtgcatg gctgtcgtca gctcgtgtcg tgagatgttg ggttaa
//
LOCUS       DAOSMP010000072         7698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_9482_length_7698_cov_21.403114, whole genome shotgun sequence.
ACCESSION   DAOSMP010000072 DAOSMP010000000
VERSION     DAOSMP010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 7698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7698
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9482_length_7698_cov_21.403114"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PKX09_09090"
     CDS             complement(<1..441)
                     /locus_tag="PKX09_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18014.1"
                     /translation="MIVPMKKVVLFVHTPERDSALKKLRKLGVVHIQHIKPPISEDTS
                     KIENAIYAVEKSLGIIEGYNTPPSSENVDLEKCQKLVSDIIALETEKQNLKSKIDEKN
                     IYLNWYNRWGIITEKDVVELKEHGIYLHLYVIDRSILKNIPEEAA"
     gene            complement(438..1049)
                     /locus_tag="PKX09_09095"
     CDS             complement(438..1049)
                     /locus_tag="PKX09_09095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOU18015.1"
                     /translation="MAKIKHTKNELKAQREALARYERFLPTLELKKNQLVTEIRHLSL
                     AIEQVEEEIRKVTEQVFAWVDVMAEEVDLKAWLKIKEIHTDEGNIAGIEIRIFRQVEF
                     EDVPYDFFTTPLWVDRALSILKEQIARRIKIKILQEQIEVLQQELRITIQRINLFEKV
                     KIPETRENIRIIQIFLGDAQTAEVVRGKIAKSKIQSKREALKV"
     gene            complement(1065..2375)
                     /locus_tag="PKX09_09100"
     CDS             complement(1065..2375)
                     /locus_tag="PKX09_09100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOU18016.1"
                     /translation="MQKVYNKILSVTGNVITVKASNVANEELAEISTQRGKSLAQVIK
                     LDGDIVSLQVFAGSQGIATNDEIRFLGHPMQVTFSDNMLGRIFNGSGVPRDKGPQLTE
                     NMIQIAGPSVNPAKRIIPRRMVRTNIPMIDVFNSLVISQKLPIFSVSGEPYNELLARI
                     ALQAEVDMIILGGIGLKYDQYMFFKNTLEEGGALSRSIFFIHTASDPIVEGLMVPDLS
                     LAVAERFALKGRDVLVLLTDMTNFADALKEIAITMEQVPSNRGYPGDLYSQLAARYEK
                     AVDFEGAGSVTILAVTTMPGDDVTHPVPDNTGYITEGQFYLRNGRIEPFGSLSRLKQQ
                     VNKSTREDHRAIMDVMIQLYAQFKETEEKRSMGFRMSDWDRKLLKYGERFENDLMDLK
                     VNIPLESALNLGWQILADCFEPQETGMKSELVQKFWPKKAEVSA"
     gene            complement(2359..4098)
                     /locus_tag="PKX09_09105"
     CDS             complement(2359..4098)
                     /locus_tag="PKX09_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOU18017.1"
                     /translation="MKKYGKITSINGNMITVACESDIIQNEVGYAVAGDENLKAEVIK
                     IQGNLAYMQVFESTKGLRVDDKVEFSGQMLSVQLGPGLLGMVYDGLQNPLHDLAAKFG
                     FFLKRGAVIEPLDSKKEWDFKPRVKSGDIVQAGISLGNVSEGIFEHQIFVPFNLRGEY
                     AVKTIVPEGKYKITETIAVITNEKGDEINLKMYFEWPVKIPITAYREKFVPTEPLVTQ
                     TRIIDTFFPISKGGTYCIPGPFGAGKTVLQQVTSRFAEADVVIIAACGERAGEVVETL
                     REFPELTDPRTGRSLMDRTIIICNTSSMPVAARDASVYTAATIGEYYRQMGLNVLLLA
                     DSTSRWAQAMRELSGRLEEIPGEEAFPAYLESRIAQFYERAGIVELLDGSRGSLTIGG
                     TVSPAGGNFEEPVTQSTLKVVGAFHGLSRDRSNARRYPAIHPLESWSKYTSFIPSNLI
                     EDARQILFKGNEVHQMMLVVGEEGTSLDDFEIYMKSEFLDAVYLQQNSFDQVDGASSP
                     ERQKYVFSKVHEVLTTKFNLTDKNDARDFFNKLRQVFIDWNYIDFKDDEFKIQEQKIN
                     QLIKEKARDAKSL"
     gene            complement(4095..4592)
                     /locus_tag="PKX09_09110"
     CDS             complement(4095..4592)
                     /locus_tag="PKX09_09110"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOU18018.1"
                     /translation="MDKYYYLIAQLPVLYFEREPALTIEQFLTEAYKWLSAEDYAQLE
                     KVDLNATAPDKNDPDIIRRIKNFELDLRLELVEWRTAQKKNQDYKIVAFPANLVREGD
                     PLTVEKNLLLWRWQFLDSLEFGHYFDLEFLIMYYLRLQILKRLFTFNKKIGREKYQQY
                     LEMEL"
     gene            complement(4585..5184)
                     /locus_tag="PKX09_09115"
     CDS             complement(4585..5184)
                     /locus_tag="PKX09_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18019.1"
                     /translation="MDVKLDSLIEKIKQEGIEEAQRASDEIRRKAEAEAQEIINKANE
                     KARQIINEGQQNAEKLRANAEAAIRQAARDTILVTKENLLKLFDRVFKKEIAKTLTPE
                     FLKEIILELVKSTSSDNQYEFVVAEKDIEKLKTLVLAATRDSLKEPVTFRVDRGIAAG
                     FRVSRKGEEVYYDLTDQSIAEVLQEFLNPALRQLLDQNG"
     gene            complement(5395..>5748)
                     /locus_tag="PKX09_09120"
     CDS             complement(5395..>5748)
                     /locus_tag="PKX09_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18020.1"
                     /translation="QIGFGTNFNWQNINFHAKALYSHTLFDFWLEGKAKLMMGIKSDP
                     LDYPYKYSLHLFDLNAEAEWRRGPLGIAYTFRQLIPTGRRMDDSPIRFSKKVPGVEYT
                     YRGGQQHQINLAYYF"
     assembly_gap    5749..5848
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5849..6675)
                     /locus_tag="PKX09_09125"
     CDS             complement(<5849..6675)
                     /locus_tag="PKX09_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18021.1"
                     /translation="MTVSLSNLAFASHWQASIYDLLAFPEIPTATFAELNSPGLPFIK
                     ARIGYDDIYRKKVDQQTTLTISEQTNTLQLNLPFRLRQTSHLIKAEAARNFSNFDNLS
                     DQTSLVSHCQIDNTYFSTLWAMAYERIHFGGGLEYNRNSAPFLINIISFPHSEDDLTN
                     KYFFDLLEPTFGEELTNTLHVNQIGYSLWTSLPLNSNYHLGLAIRATDFDFHWRIRYI
                     NTGQEAALAGQRQIDLPINGLNHYYRLALSSDQGLVRDISVTYLRNDFDYFIDNNPP"
     gene            complement(6744..>7698)
                     /locus_tag="PKX09_09130"
     CDS             complement(6744..>7698)
                     /locus_tag="PKX09_09130"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU18022.1"
                     /translation="KEYGYSQNPYADPNPYVLDANSEFSLTFLAVPQVKIATAAIEKA
                     CEGLSKIGDVLNGFYSELDQMFILDLDPNYLDFSEVAGPMDVISILEQSNPEFLTVTD
                     YGVAQFHEMGDKLEDSFNSLTIFFNNLTGLFEAMEPYQDDFGIDAPKMQGISTKSAEM
                     AGSLHRDFANPDSTIIIDGERVNVSAWFDNPPASFLVMVKNFFMGTDSTLGGMFPDRF
                     KVGIAQENQALPTEFKLYPVYPNPFNPVANITFDLPHTADVKLCIVDLQGRVIEQIVN
                     RRISAGRISYDWNASHYPSGIYFALLEVNGHRSIRKMTLLK"
BASE COUNT         2101 a         1720 c         1583 g         2194 t
ORIGIN      
        1 ggccgcttct tccggtatat ttttcaatat actacgatca attacatata aatgcaggta
       61 tatcccgtgt tcttttagtt caacaacatc tttttcggta attatgcccc agcggttata
      121 ccagtttaag tagatgtttt tctcatcaat tttcgatttc agattttgtt tttcggtttc
      181 gagagcgata atatcggaaa ccaatttctg acacttttct aaatcgacat tttctgacga
      241 cggcggtgta ttatagccct cgataatgcc cagacttttc tcgacagcat atatggcatt
      301 ctcaattttt gaggtatcct ctgaaatcgg cggcttgata tgctggatgt gaaccactcc
      361 taatttgcgt aatttcttca gagcggaatc tcgttccgga gtatgcacaa aaagcactac
      421 ttttttcatc ggaactatca tacctttaac gcctctcttt tactctgaat cttagattta
      481 gcaatcttac cgcgcactac ttccgccgtc tgcgcatcgc cgagaaaaat ctggattatg
      541 cggatatttt cccgagtttc gggaatttta actttctcga acaagttaat gcgttgaatg
      601 gtgatgcgta attcttgttg tagaacttcg atctgctcct gtaaaatttt aattttaatc
      661 cggcgtgcga tttgctcttt aaggatgctg agagcgcgat caacccacag aggcgttgta
      721 aaaaaatcat agggcacatc ttcaaattca acctggcgga aaatccggat ttcgattccg
      781 gcaatattgc cttcgtcagt gtggatttct ttgattttca accaggcttt taaatctacc
      841 tcttcagcca taacgtctac ccaggcaaac acctgttcag taactttcct gatttcttcc
      901 tcaacctgtt cgatagcaag actaagatgc cggatttcag taaccaattg atttttcttt
      961 aactcgagag tcgggagaaa gcgctcgtag cgtgctaagg cttcgcgctg agcttttaat
     1021 tcatttttgg tatgtttaat cttagccatg attatttata tgaatcaagc gctcacttct
     1081 gctttctttg gccagaattt ctgcaccaat tccgatttca ttcctgtctc ctgaggttcg
     1141 aaacaatctg ccagaatttg ccagccgagg tttagagctg attcgagcgg aatattaacc
     1201 ttcaaatcca tcaaatcgtt ctcaaaccgc tcgccgtatt tcagcaactt gcgatcccaa
     1261 tcactcatcc ggaatcccat cgagcgcttc tcctcagttt ccttgaactg agcgtaaagc
     1321 tggatcataa cgtccataat ggcgcgatga tcttcccgcg tacttttgtt gacttgttgt
     1381 tttagacgac tgagtgaacc aaaaggctcg atgcgaccgt tgcgcaagta aaattgtcct
     1441 tcagtaatat aaccggtatt gtccggaacg ggatgggtaa catcgtcgcc cggcatagtc
     1501 gtaaccgcca gaatggtcac cgaaccggcg ccttcgaaat caactgcttt ttcataacgc
     1561 gcggctaact gactataaag atcgccggga taaccacggt tggatggaac ctgttccatc
     1621 gtaatggcaa tttccttgag cgcatcggca aaattagtca tatcggtcag caaaaccagc
     1681 acatcacgtc ctttgagtgc aaagcgttct gccacggcta aactcaaatc cggcaccatt
     1741 aaaccctcta cgattggatc ggaagccgtg tgaatgaaaa atatcgagcg gcttaaggcc
     1801 ccaccttctt cgagcgtatt tttaaaaaac atatactgat catatttcag accgatgccg
     1861 cccagtataa tcatatcaac ttccgcctgt aatgcaatgc gcgccaaaag ctcgttgtat
     1921 ggttcaccgg aaaccgagaa aatcggcaat ttttgcgaaa taaccagaga attgaacaca
     1981 tcaatcattg ggatattggt tctgaccatt cgcctgggta tgatgcgctt agccgggttg
     2041 accgaaggac cagcaatctg gatcatattt tctgttaatt gtggaccttt gtcgcgcggc
     2101 acgccactgc cattaaaaat gcgaccgagc atattatccg agaaagtaac ctgcatcgga
     2161 tgccctagga aacggatttc atcgttggtg gcgatgccct gactgccagc gaatacctgg
     2221 agcgaaacaa tgtcgccatc caatttaatg acttgcgcca gagatttacc gcgctgggta
     2281 gaaatttccg ccagctcttc gttagctacg ttgctggctt ttactgttat tacgttgccg
     2341 gttacgctta aaattttatt atagactttt tgcatcgcgg gctttttcct tgatcaattg
     2401 atttattttc tgttcttgta ttttgaattc atcgtcctta aaatcaatat aattccagtc
     2461 aatgaagact tgccgcaatt tattgaaaaa atcgcgggcg tcgtttttat cagtcagatt
     2521 aaatttggtc gtcaatacct catgcacctt gctgaagaca tatttctggc gctcggggct
     2581 actggcgcca tctacctgat caaaagaatt ctgctgtagg taaactgcat ccagaaactc
     2641 agatttcata taaatttcaa aatcatctaa ggaagtgcct tcctcaccga ctaccagcat
     2701 catctggtgc acttcattac ccttgaaaag tatttggcgc gcatcctcga ttaaattcga
     2761 gggaatgaaa ctggtgtatt tcgaccagct ttctagggga tgaatagccg ggtaacggcg
     2821 ggcattggaa cgatcgcggg aaagcccgtg aaaagcgccg acaactttca aggttgattg
     2881 agttaccggc tcttcgaaat taccgcctgc cgggctgacg gtacctccga tggttaaact
     2941 accacgacta ccatcgagca actcgacaat tccagcgcgt tcgtaaaact gggcaatgcg
     3001 tgattcaaga taagccggga aggcttcttc tcccggaatt tcttccagac gaccggatag
     3061 ttcccgcatt gcctgcgccc aacgtgaagt ggaatccgct agtagtaaaa cattcaatcc
     3121 catctggcga taatattcac caatcgttgc ggccgtataa accgaggcgt cgcgggcggc
     3181 aaccggcatt gaactggtat tgcaaataat tatggtgcga tccattaggg agcgaccggt
     3241 gcgcggatcg gtcagttcag gaaattcgcg cagagtttca actacttcac cagcacgttc
     3301 accgcaagcg gcgatgatta cgacatcggc ttcagcaaag cgactggtca cctgttgaag
     3361 taccgtctta ccagcaccga aaggtcccgg aatacaatag gtaccgcctt tggaaatcgg
     3421 gaagaaagta tcaatgattc tggtctgcgt caccagcggt tcggtcggta caaatttctc
     3481 acgataggct gttattggaa tcttgactgg ccattcgaaa tacattttca aattgatttc
     3541 gtcacctttt tcattggtaa ttacggcaat agtctcggtt attttatact tgccctccgg
     3601 cacaatcgtt ttgaccgcat attcgccacg caggttgaaa ggcacaaaaa tttgatgctc
     3661 aaaaataccc tccgagacat tgccgaggct gataccagcc tggacaatat cgccggactt
     3721 gacccgcggt ttaaaatccc attccttctt tgaatccagc ggttcgataa cagctccgcg
     3781 ctttaggaaa aagccgaatt tagccgccaa gtcgtgcaaa ggattctgga gtccgtcata
     3841 aaccataccg agcaatccgg gaccgagctg caccgaaagc atttgtccac tgaattcgac
     3901 tttgtcgtcg actctcagac ctttggtgct ttcgaagacc tgcatataag ccagattgcc
     3961 ttgaatcttg atcacctctg ctttgagatt ctcatcgcca gccacagcgt aaccgacttc
     4021 attctggata atgtcgctct cacaagcgac ggtgatcata ttgccattaa tgctagtaat
     4081 ttttccgtat tttttcataa ttccatttct aaatattgtt gatatttttc acgtccgatt
     4141 tttttattga aagtaaatag tctttttaaa atctggagtc gtagataata cataatgaga
     4201 aattccagat cgaaataatg accgaattcc agcgaatcta aaaattgcca gcgccatagt
     4261 aagagattct tttcaacggt taagggatcg ccctctcgca ctaaattagc aggaaacgct
     4321 acaattttat aatcctgatt cttcttctgg gctgtccgcc attcaactag ctcgaggcgt
     4381 aaatctaatt caaaattctt gattcgacga ataatatcgg gatcgttttt atccggcgct
     4441 gtggcattca aatccacctt ttccaattgc gcataatcct cagccgaaag ccatttgtag
     4501 gcttcggtta aaaattgttc gatggtcagc gccggttctc gctcaaaata taaaacaggc
     4561 aactgagcga ttaaatagta atatttatcc attctgatct aataattggc gcagagccgg
     4621 attgagaaat tcctgtaaaa cttcagcgat gctttgatcg gttagatcat aataaacctc
     4681 ttcacctttg cgcgaaaccc ggaagccggc ggcaatacca cggtcaactc ggaaagtaac
     4741 cggctccttg agactatcgc gagtcgccgc caataccaaa gtcttcaatt tctcaatatc
     4801 tttttcggct accacgaatt catactggtt gtcggaagat gtagatttta ccaattccaa
     4861 aataatttct tttaaaaatt ccggcgtcag tgttttggct atttctttct taaaaacccg
     4921 atcgaaaagt ttcaggagat tttctttagt tacgaggatt gtatcacggg cggcttggcg
     4981 aattgcggct tccgcgttag cgcgcaactt ttcggcattc tgttgacctt cattgataat
     5041 ctggcgggct ttttcattag ccttattgat aatttcttga gcttcggctt cggctttccg
     5101 gcggatttca tcggaagcgc gctgcgcttc ttcgatgcct tcctgtttta tcttctctat
     5161 taaactgtcc agtttaacgt ccataaaaat ctcctatagt tcggcttctt attcattaga
     5221 aaataacttg tgaatttaac ttctgtgaat tattaagtca agcaaggttg aagatttttt
     5281 ggataacatc aagacagaat atccaccatt tgaagataaa taggattaat cccaaacgat
     5341 ttctaaaaac ccttacttcg taagcaagaa atataattcc tgctttttgg atgattaaaa
     5401 ataataagcg agattgatct ggtgctgttg gccaccacgg taagtgtatt ccacgccggg
     5461 cactttttta gaaaaacgga tcgggctatc atccattctg cgaccggtcg gtattagttg
     5521 ccgaaaagta taggcaattc cgagcgggcc tcgacgccat tctgcttcgg catttaaatc
     5581 gaataaatgg agactatatt tgtaaggata atccagcgga tccgatttaa tacccatcat
     5641 caatttggct ttcccttcta accagaaatc gaataaagta tgagaatata aagccttggc
     5701 atgaaagtta atattctgcc agttgaaatt tgtcccaaaa ccaatctgnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt cgggggattg ttatctatga aataatcgaa
     5881 gtcattgcgt aaataagtca cagaaatatc tcttaccaaa ccctggtctg aacttaaagc
     5941 aagtcggtaa taatgattta atccattaat tggaaggtca atttgccgtt gaccggctaa
     6001 agccgcctct tgaccggtgt tgatataacg aatacgccag tggaaatcaa aatcagttgc
     6061 ccgaattgcc aaaccgagat gataattgct attcagagga agtgaagtcc atagtgaata
     6121 accgatttga ttaacgtgca aggtgttagt caattcctct ccaaaagtcg gttccagcaa
     6181 gtcgaaaaag tatttattag taaggtcatc ttcggaatga ggaaacgaga taatattaat
     6241 taaaaagggg gctgagttcc ggttatattc caagccgcca ccgaagtgaa tacgttcgta
     6301 agccattgcc cataaagtcg aaaaataagt attatcaatt tgacaatgac tgaccagact
     6361 ggtctgatcg ctgaggttat caaaattgga aaaatttctt gccgcctcgg ctttgattag
     6421 atgagatgtt tgccttagac gaaaaggcaa attgagttgc aaggtattcg tctgttcgga
     6481 gatggtcaaa gtcgtctgtt gatcaacctt tttgcgataa atatcatcat aaccgattcg
     6541 ggctttaata aaaggcaaac ccggtgaatt caattcggca aatgttgcag ttggaatttc
     6601 agggaatgcc agtaaatcgt aaatcgaagc ctgccaatgg ctggcaaatg ccaaattgga
     6661 cagactgaca gtcaaaaaaa aagtggggaa aaggtagtgg cttttcccca caaaaaactg
     6721 atggatcagt cgcaactctg gtcctattta agcagagtca ttttgcggat gctccggtga
     6781 ccattgactt ccagcaaggc aaaataaatc ccggatggat aatgggaagc gttccagtcg
     6841 taggaaatcc gacccgccga tatcctgcga ttgacgatct gttcgattac tcgtccctgc
     6901 agatcaacaa tgcacagttt gacatcagcg gtatgcggca aatcaaatgt gatattcgcc
     6961 actggattaa aggggttagg ataaactgga taaagcttga attcagtcgg taaagcttga
     7021 ttttcctgcg cgatgccgac cttaaaccgg tcaggaaaca ttccgcccag agtgctgtcg
     7081 gtgcccataa agaaattttt caccataacc aggaaacttg ccggcggatt atcgaaccag
     7141 gctgaaacat tgactcgctc gccgtcaatg ataatagtgg aatccgggtt cgcaaaatca
     7201 cgatgaagac tcccagccat ttcagccgat ttagtcgaaa tcccctgcat tttcggagca
     7261 tctatcccaa agtcgtcctg atatggttcc atcgcttcga aaaggccagt tagattatta
     7321 aagaaaattg tcaaactatt gaatgaatcc tcgagcttgt cacccatttc atgaaattga
     7381 gcgacgccat aatcagtaac ggtcaaaaat tcgggattag attgttccaa tatgctgatt
     7441 acatccatcg gtcctgcaac ctcggaaaaa tccaggtagt tcggatcgag atccaaaata
     7501 aacatctgat cgagttcgct ataaaaaccg tttaaaacat caccgatttt tgacaaacct
     7561 tcgcaagcct tttcaatcgc ggcggtggca atttttacct gtggaacggc aagaaaagtg
     7621 agtgaaaatt cactgttggc atctaaaaca taaggatttg gatctgcgta tggattctga
     7681 ctatatccgt actctttc
//
LOCUS       DAOSMP010000073         7086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_10645_length_7086_cov_21.001991, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000073 DAOSMP010000000
VERSION     DAOSMP010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10645_length_7086_cov_21.001991"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PKX09_09135"
     CDS             complement(<1..1064)
                     /locus_tag="PKX09_09135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU18023.1"
                     /translation="MIIIHLITFISIVIYLVSLIIFRWGLNHPRQQRTADLPTISIVI
                     AVKNEAVNLPRLIEQLTGQNYPDGKLEIIIVDNDSTDETWAILQNEAARISNLRIFTT
                     RTEGTPYRHKKAALAVGIREARGEVILTTDADCSMGKDWARTMASYFTDDVGIVVGFS
                     AIRYLNDWFSRLQALDYLQLMAAAQGSTNLGLAWACSGSNWAFRKVLFENAGGYMLVR
                     DRVGGDDSLFMQVMQKRTRTKVVFAADKAAWVETQAADSLATFLRQRIRWASEANYMH
                     HFNCLFFVVILATFLANLMPIVYFVLAITGVVTFVPFLIVIAIKLIGEAMLAFKAVRV
                     YQRKDLMGVFIPWFIIQPFY"
     gene            complement(1083..1982)
                     /locus_tag="PKX09_09140"
     CDS             complement(1083..1982)
                     /locus_tag="PKX09_09140"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOU18024.1"
                     /translation="MINNHQYFEPELKPHPPIIVPKIKTAIGLLIMGLICATLVSLMI
                     MIFVPTSNLTKTTQLLLLIAELFLPVPIFFWAWRRHTNLGELLRINRVSSPSIIAAMI
                     VSVSLTVIIDELDRLIQIVLPLPESFASIGEIMLITDWQSALLIIGVVVFAAPLVEEM
                     MFRGFFQRILEYRQGDITKSVLLSAMVFALVHFNPWWVVQIYLMGLFLGYLAWRANSI
                     WPSFVLHAVNNGWSVWWSHQPAFAKFYEWHGHVHPVLIIASLVCFYLGMRFFIKVTPV
                     VEKRQDIVLIENLSDYYENEPPE"
     gene            complement(1994..2539)
                     /locus_tag="PKX09_09145"
     CDS             complement(1994..2539)
                     /locus_tag="PKX09_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOU18025.1"
                     /translation="MAFLGYVLAIIFLSVTPASPQVTVDTLAIGTVAPSFYASVLDGS
                     DFFLSHKVGAKARPNEKAPVVLVFFTTSCIPCRQEIPFLDSLRIEYPQIAFYLVNVGE
                     ESQIVKEYIQRMNYKMPVLLDRYGQVAKRYQANITPTLVGINIIGQIAFLKRGFQVSD
                     KELIQTNIKKLTDANLFIEKN"
     gene            complement(2551..3318)
                     /locus_tag="PKX09_09150"
     CDS             complement(2551..3318)
                     /locus_tag="PKX09_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOU18026.1"
                     /translation="MILTRNERALYRSLKQKKERERTGLFVIDGIKPVQEALIAAFPL
                     ERILISESIDEAARLSLKSLADKQGIAIIVVTQSEIEQISALRNPEGVLAIAKIPSEV
                     DLLATLDYPALYLWQINDPGNLGTIMRTALWFDVKTILLSPDSVDVYSPKVVRGAMGA
                     LFRLTVRTGVDLSRIEMILGRDNAQMWAADMSGTIEKPEITNDRFVIAFGSESHGLPP
                     EILQRATGLIRINKYGYGESLNVAVAVGIILNNIRQK"
     gene            complement(3321..5396)
                     /locus_tag="PKX09_09155"
     CDS             complement(3321..5396)
                     /locus_tag="PKX09_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOU18027.1"
                     /translation="MSAKVKSLTKRISSFFFSLRMQIANNPLNEYIRLLFFAFLIGVL
                     AGLVTLAFYKLIHLISYFLYDYLPARYNNLPKWTMALFPAVGGLIVGLMIKLWPRQPR
                     QRGVVEVIKASFIGRGVIRVTTIIFNFIATAIFLGTGGSLGPEAPVAFLGAGSGSLIG
                     QAFRVSENQRRVFLAAGTAGAIAAVFNAPIGGVFFAIEIVLFNNFQTLTFSALIISAI
                     TADYISRVFLGDQLLLTLPKVEMALSLNILYFAVLGIITGLFSLAFIRFSGKMHNFFD
                     AQKGRFAPLLRLTGLMLILGIVGLFFPQIIGIGYDAIQEIVNNNYTWQILAIVFVLKF
                     LFSGLLSAAGGFGGLFAPSLLMGAALGAFLAKGGNQVLGLSLDVAGFAIAGMGGMLAA
                     VNSIPVTALLIIVEITGNYHLVLPLMLTVIMSYIIVHYVLKETVYSIELRQRGIQLFD
                     GKELNVLKNVKIDQIMSYEFHKVYSTMDVKTLVKVIAEGAANIYWVENEQHKIIGFIS
                     WSDLKPLITDYQNLNTLVLVSDVMNPNVVSVKTDDTLDYVMKLFGRYNVDELPVIDPA
                     TGTLIGTVNRSRVIQLYNQEIFKRETAGDLATHIRYLDQTRFVEIDEDFSILEMNAPE
                     SMVNSSLKALDLRNHYHAQIILIKRLDNKGRRIVITPRADTIILPNDILILAGPTKKL
                     YELKERYYL"
     gene            complement(5723..6901)
                     /locus_tag="PKX09_09160"
     CDS             complement(5723..6901)
                     /locus_tag="PKX09_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOU18028.1"
                     /translation="MFKEKIRQAIEILKEQGVDMWMTFVRETETLPDPCMDLIVGTNC
                     TWQSAFIITAKGQTIAIVGSLDKERIKQTGLYDDIRIYVDDFGAAFLNALNEINPNRI
                     AINTSINDYMADGLTHGLYELLMGYTKNTPYRERFISSEKIVAALRGRKSPTEIQRIK
                     RAIEITEKIYAEISPQIKSGRTEKEIADIILKMVAELGVQTAWESNSCPAVFTGPLSA
                     GAHAEPTERVIQPGHVLNIDFGVKKDGYCSDIQRTWYILRNNEKTAPEPVQKGFAVLL
                     ESVRRAVDAIRPGKMGYQIDQIARDYITANGYPEFPHGLGHQVGRSTHDGAGMLGPRW
                     ERYGDTVYLPLEIGQVFTIEPRLPIEGFGVATVEEMIVVTADGCEYLSHPQNQLFYSR
                     "
BASE COUNT         2128 a         1577 c         1452 g         1929 t
ORIGIN      
        1 atataaaatg gttggatgat aaaccagggt atgaaaacac ccatcaagtc ttttctttga
       61 taaacccgga ccgctttaaa agctaacatt gcttcgccga ttaattttat tgcaattaca
      121 attaagaacg gaacgaaggt aactactccc gttattgcaa ggacaaaata gacaatcggc
      181 atcagattag ccaaaaacgt tgctagaatt acgacgaaaa ataggcaatt gaaatggtgc
      241 atgtaattag cttcgcttgc ccagcgaatg cgttgacgta gaaaggtcgc aaggctatca
      301 gcggcttggg tttccaccca ggcggcttta tccgcggcga atactacttt cgtgcgggtg
      361 cgtttctgca taacctgcat aaagagtgag tcgtcgccgc caactcgatc gcggaccagc
      421 atatagccgc cagcattctc aaatagtact ttacgaaaag cccagtttga accactacaa
      481 gcccaagcca agccgagatt ggtcgagcct tgggcggcgg ccatcaattg cagataatcc
      541 aaagcctgaa ggcgcgaaaa ccagtcattg agataccgaa tagccgaaaa tccgacgacg
      601 attccgacat catcagtaaa ataactcgcc atcgttcgcg cccagtcttt tcccatggaa
      661 cagtctgcat ccgtagtcag gattacctcg ccgcgggctt cgcgaattcc taccgctaat
      721 gcggcttttt tatgacgata cggtgttcct tcggtcctgg tagtaaagat ccgcaggtta
      781 ctaattctag cggcttcgtt ttgtaaaatt gcccaagtct catcagttga gtcattatca
      841 acgatgatta tttcaagttt tccatctgga taattctgtc cggtcaattg ttcgatcagc
      901 cggggcaggt tgactgcttc atttttgacc gcgatgacaa tgctgattgt gggtaaatca
      961 gcggttctct gttggcgtgg gtgatttagt ccccaacgaa aaatgatgag gctgactaaa
     1021 tagattacaa tgcttataaa tgtaatcagg tgaattatta tcattgcaac ataatttgga
     1081 tattattcag gaggttcgtt ttcatagtaa tcgcttaggt tttcgatcaa gactatatcc
     1141 tgacgttttt ctacaaccgg cgtaaccttg ataaaaaacc gcattcctaa atagaaacat
     1201 actaaactgg cgataattag gactggatgg acatgaccat gccattcgta aaacttagcg
     1261 aatgccggtt ggtggctcca ccaaaccgac cagccgttat taacggcatg taaaacgaac
     1321 gaaggccaga tcgagtttgc ccgccaggct aaatacccca aaaaaagtcc cattaggtaa
     1381 atctggacaa cccaccaggg attaaaatga accagagcga ataccatcgc tgagagtaag
     1441 actgatttgg ttatatcacc ctgacgatat tctaaaatgc gttggaagaa accacggaac
     1501 atcatctcct cgactaaagg agcggcaaat acaaccacac cgataatcag cagagccgat
     1561 tgccagtcgg ttatgagcat aatctcacca attgacgcaa aactctccgg taatggcaaa
     1621 acgatctgga tgagtcggtc caattcatct ataatgactg tcaaactaac cgaaacaatc
     1681 atcgccgcga ttatagaagg tgaagaaact cgattgattc ttagcagttc acccaaatta
     1741 gtgtgcctcc gccaagccca aaagaaaatc gggactggta aaaacaattc cgcaatcaat
     1801 aataacaact gagtagtttt tgtcagatta ctcgtcggca caaaaatcat tatcattaag
     1861 gaaaccagtg tcgcgcatat tagccccata attaatagtc caattgctgt tttaattttg
     1921 gggacgatta ttggtggatg gggtttcaat tccggctcaa aatactggtg attatttatc
     1981 attaatttgc gtcctaattt ttctcaataa acaaattggc atcggttagt tttttgatat
     2041 tggtttgaat tagctctttg tcactgactt gaaaaccgcg cttgagaaag gcgatttgtc
     2101 caatgatatt aatgcctacc agagttggag tgatattagc ctggtagcgc tttgccacct
     2161 gtccataacg atcgagcaga accggcattt tatagttcat tctttgtata tattctttaa
     2221 caatctggga ttcttcaccg acattgacca gataaaaggc aatttgtgga tattcgatcc
     2281 ttaagctgtc gaggaaaggt atttcctgcc gacaggggat acaggaagtt gtaaaaaaga
     2341 caagaactac cggtgctttt tcattcggac gagctttagc gccgacttta tggctaagaa
     2401 aaaaatccga accgtcgaga acactagcat agaagctcgg cgctaccgta ccgattgcta
     2461 aggtatcgac ggtgacctgg ggtgaggctg gggttaccga taagaatata attgctaaaa
     2521 catagccaag gaaagccaag ttcgatcctc ttacttttgc cgaatattat ttaaaatgat
     2581 tccaactgcc acggcaacat tcaaagattc gccataaccg tatttattaa tgcgaattaa
     2641 tcccgtggcg cgctgtaata tttcaggcgg taagccgtgc gactcacttc cgaaggcaat
     2701 aacaaaacgg tcatttgtta tttcaggttt ttcgatggtg ccggacatat ctgccgccca
     2761 catttgggca ttgtcccgcc ctaaaatcat ctcgatccga gacaggtcaa ctcccgttct
     2821 cacagtcagt cggaaaagtg ctcccatagc gccgcgaacg actttaggac tataaacgtc
     2881 aaccgaatcg ggcgaaagca ggatagtttt tacatcgaac caaagtgccg tacgcatgat
     2941 tgtgccaaga ttgcccgggt cattaatctg ccagagatag agcgccggat aatcgagagt
     3001 cgcgagcaaa tctacttcgc tcggaatttt ggcaatggcg agaactcctt ccggattacg
     3061 gagcgcagaa atctgctcga tttccgattg agtaactaca ataatcgcga ttccctgttt
     3121 atcagccagg gatttcaggc ttagccgtgc ggcttcgtca atgctttcgg aaattaaaat
     3181 gcgctcaagg ggaaatgcgg caatcaaggc ttcttggacc ggtttgattc catctatcac
     3241 aaatagtcca gtacgctccc gctccttttt ttgctttaaa gaacggtaaa gtgctctctc
     3301 gttacgcgtc aggatcattt tcaaagataa tagcgctctt ttaattcgta taattttttg
     3361 gttgggccag ctagtatcaa gatatcgttt ggcaggataa tcgtgtcggc gcggggtgtg
     3421 atgacgattc ttcggccttt attatccaac cttttaatta aaataatctg agcatgataa
     3481 tggttacgca gatcgagtgc ttttagactg gaattaacca tagattctgg agcattcatt
     3541 tccaaaattg aaaaatcctc atcaatttct acgaagcgag tttgatcgag atagcgaata
     3601 tgagttgcca gatcgccggc cgtttcgcgt ttgaaaattt cttgattata caattggatg
     3661 acgcgactac ggttaaccgt tccgattaac gttccagtgg cgggatcaat caccggcaat
     3721 tcatcaacat tataacgccc gaacagtttc attacataat cgagagtatc gtcggtttta
     3781 accgatacca cattaggatt catcacatcc gagacgagta ccaaagtatt caaattttga
     3841 taatcggtaa tgagcggttt taaatccgac catgagataa agccgataat cttatgctgt
     3901 tcattttcta cccaatagat attggctgcg ccttccgcaa tgactttcac cagagttttt
     3961 acatccatag ttgaatagac tttatggaat tcatacgaca taatctgatc aatctttaca
     4021 ttttttaaga cattcagctc cttgccgtca aataattgaa tgcctctttg tctcaattcg
     4081 atcgaataga cggtctcttt tagcacataa tgaacaatta tataactcat aatgacggtg
     4141 agcatcaagg gtagaacgag atgataattg ccggttattt caacgatgat taataatgct
     4201 gtcaccggga tgctgttgac ggcggcgagc ataccaccca ttcccgcaat ggcgaaaccg
     4261 gctacgtcca gcgaaagacc caatacctga ttgccgccct tagccaggaa tgcgccaagc
     4321 gccgcaccca taaggagtga aggggcaaat agaccgccaa aaccacccgc ggctgacaac
     4381 aatcctgaaa ataaaaattt aagtacgaat acaattgcta gaatctgcca agtataatta
     4441 ttattaacaa tttcttgaat ggcatcatag ccaatgccaa taatttgggg gaagaaaagc
     4501 ccgacaatgc ccaatattag cattaaaccg gtaagcctga gtaacggtgc aaaacgacct
     4561 ttctgagcat cgaaaaaatt gtgcatttta cccgagaaac gaataaaagc caatgaaaat
     4621 aaaccagtga taatacctaa aaccgcaaaa tataatatat tgagggataa agccatttca
     4681 acttttggca gagtcaaaag cagttgatca cccagaaaaa ccctcgagat gtagtcggct
     4741 gtaatggctg agattattaa agccgagaag gtaagcgtct ggaaattatt aaataaaaca
     4801 atttctatgg caaaaaaaac tccaccgatg ggagcattaa aaacagcggc tatcgcacca
     4861 gctgtacccg cggctaagaa aactcgcctt tgattttccg acactcgaaa agcttgaccg
     4921 attaatgaac cactaccagc tcccagaaat gcgaccggcg cttccggccc cagacttcca
     4981 cctgttccga gaaaaatcgc cgtcgcaata aaattaaaaa tgatagtagt aactcggatt
     5041 acaccccgtc cgatgaaact ggctttgatt acttctacta ctccgcgctg acgcggttga
     5101 cgcggccata gtttaatcat taagccaaca atcagccctc cgaccgccgg aaaaagagcc
     5161 atcgtccatt tcggcaaatt attatacctt gcaggtaaat aatcgtatag gaaatagctt
     5221 atcaagtgga ttagtttgta aaaagctaga gtcaccaatc cagccaaaac cccaatcaga
     5281 aaggcaaaaa acagcagacg gatatattcg ttaagtggat tattagcaat ctgcatccgc
     5341 agggagaaga aaaagctgga tatccgcttg gttaaagatt taaccttagc actcacacta
     5401 aaactccgct atgtttatca atatcaagta attatatttt ttataaaatg tcatttgtta
     5461 tatttatctg ccagttatca aagacacttc gcgaatctat cataaaataa agtcaaaaat
     5521 caaggcaaaa ttagtctgcc ggtggctatc aaacaagtcc ggtgttgatt taaccgttat
     5581 catttccccg cttagttcaa aatcaaatct tccaaaatta aatcttaaaa tgtctccaaa
     5641 gaattcccgc cgaaaactcg ggatggtcga agttttcatt tcgttataaa aggagaagaa
     5701 aaagtaagtt atagtatagc actcagcggg agtaaaataa ctggttttgc ggatgactca
     5761 aatactcgca cccgtcagca gtaaccacga tcatttcctc gaccgttgcc actccgaaac
     5821 cttctatcgg caaacgcggt tcgatcgtaa aaacttgacc aatttccagc ggtaaataaa
     5881 ccgtatcgcc atagcgttcc cagcgtggtc ccaacatccc ggcaccgtca tgagttgatc
     5941 gacctacctg atgaccaagg ccatgtggaa actcgggata gccgttggca gttatataat
     6001 cgcgggcaat ctgatcgatc tgatagccca ttttccccgg tcggatggca tccacagctc
     6061 gacggacaga ctccagaagt accgcaaagc ctttttgaac aggttcaggc gcggtttttt
     6121 cattatttct taaaatgtac caagtgcgct ggatatctga acagtagcca tcttttttca
     6181 caccgaaatc aatgttgagc acatgaccag gctgaatgac tcgctcggtt ggttcggcat
     6241 gcgccccagc cgaaagcggt ccggtaaata ccgccggaca ggaattcgat tcccaggcag
     6301 tttgtacccc aagttcagct accattttca aaataatatc cgcaatctcc ttttcggttc
     6361 ggcctgattt aatttgcgga ctgatttccg catatatttt ttcagtaatt tctatcgctc
     6421 tctttattcg ctgaatttca gtgggcgatt ttcgcccgcg tagcgccgca acaatttttt
     6481 cggaagaaat aaaacgctct cgataaggtg tgtttttagt atagcccatc agtaattcat
     6541 acaggccatg cgttaagcca tctgccatat aatcgttgat agaggtattg atagcgattc
     6601 gattaggatt aatttcattc agtgcgttca gaaaagccgc gccgaaatcg tcaacgtaaa
     6661 tccgaatatc atcatataat ccagtctgtt taatgcgttc cttatccaga ctaccaacaa
     6721 ttgcgatggt ttgtcctttg gctgtaatga tgaaagccga ttgccaggta caattagttc
     6781 caacgatcaa atccatacag gggtcaggta gagtttcagt ttcgcggaca aacgtcatcc
     6841 acatatcaac accctgttct ttgaggattt cgattgcctg tctgattttt tctttaaaca
     6901 ttatttctcc aatcgaattt ttgtctcgct tggcaactta aataaaatcg gagatttttt
     6961 ccaaaattaa ttgaaacata ttgagtaccg gattatggta cgtcaaccgc ttaatattaa
     7021 tttaatttat ttaacctgaa taattcataa aaataaagat gtttggttgt ttaatacatt
     7081 gtaatg
//
LOCUS       DAOSMP010000074         7063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_10690_length_7063_cov_14.628853, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000074 DAOSMP010000000
VERSION     DAOSMP010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10690_length_7063_cov_14.628853"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(700..1542)
                     /locus_tag="PKX09_09165"
     CDS             complement(700..1542)
                     /locus_tag="PKX09_09165"
                     /inference="COORDINATES: protein motif:HMM:NF024954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU18029.1"
                     /translation="MIEINYYETYWEMKNCYDTRFKIVRYAQNNNVSMAAVQFGTTRK
                     TVRKWLSRYLKEGTVGLIDRNRRPNQSPYKIDAQEEERILAIRRRQLYLGPIRIKAEH
                     GIHSSPATIHRIIKNAGLIRPRKHKTQVKRDLRIVKAQIKVFEKIQIDLKELCDIPHY
                     YPYLWQNYPKYQITAREVRAGLIYIGYAYEKTTTNVAIFTYLLGCHLLSCGVDLNQTV
                     WQSDNGSEFIGPWNQKHKLTLYQLIVQQMNPESIQIPVGRKTYNRDVEAAHRLIEDEF
                     YDMV"
     gene            1784..2857
                     /locus_tag="PKX09_09170"
     CDS             1784..2857
                     /locus_tag="PKX09_09170"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU18030.1"
                     /translation="MKIRLATNEDNQSLLALARESPMESHLIINVDRAPDYFLLANLQ
                     GDDARVFVAEQDGFIVGAIGCCLREVLLTGQPTRIGYIGGIKVAAAARKGLTSFRLMN
                     AVANYLRAVPVEMAIVITMTDNEAMAPILAGRVGMPPFHPINRLVINYIFPLFRPQVT
                     GGYQIRSFRESDLDHLTRLFTDFFRNYSLTFEWTPKRLSALLNQPNFSSENILIATKN
                     GRPVAALTWWDQSSFKRTIVEKYDGWLKTLAGLSKPLKIFPQAGEPLIELQIRHIVYE
                     KEHTSAARDLIRYLINEKKRKYRLFRIGFQQNSPLAALLSGLPHLKIHLNCYIASDKK
                     DDETRLLISGLRQGQIWEDLSLH"
     gene            2854..3795
                     /locus_tag="PKX09_09175"
     CDS             2854..3795
                     /locus_tag="PKX09_09175"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOU18031.1"
                     /translation="MKKKKSISIICNLQACGGKARRRWLDFLKIATQAGFSIDFHITE
                     YHGHATVLARERLAKGADRIIVFGGDGTLNEALNGIIVKDRPVNPDLRLVYLTAGSSC
                     DVAKMFPNPQTLLQRIADGKDYSVDVGKLECLNARAEKVVRYFLANSSIGVISRSIEK
                     FNQKRPTMNLLKRINIDLAAVSAGIKTIIQFENIDSRISYENQPFTIQKIKNITVFKS
                     PYFGGGMNYGVPTSYNDHFLHVATIAAMDAVHTFRMIPLLYSGTILKTPQSEYCRVQN
                     LSFDAPGQHIPIEADGEIVGFPPCHYAILPELIHLVV"
     gene            complement(3917..3989)
                     /locus_tag="PKX09_09180"
     tRNA            complement(3917..3989)
                     /locus_tag="PKX09_09180"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3954..3956),aa:Thr,seq:cgt)
     gene            4272..4568
                     /locus_tag="PKX09_09185"
     CDS             4272..4568
                     /locus_tag="PKX09_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18032.1"
                     /translation="MKTDRNEGWEQSVMTEIECVTIARKLQKSRRDWLSRFIVTCFYL
                     IILTFKISFTQPITVEGSVITTNLLPVKYASVTFINQNDTTQKYSAITDTCGYY"
     gene            4608..4895
                     /locus_tag="PKX09_09190"
     CDS             4608..4895
                     /locus_tag="PKX09_09190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU18033.1"
                     /translation="MPSNIELAQNYPNPFNPLTTIAYDLPQESQVMLTIYDILGREVI
                     RLVDERQPAGIQKVIWDGRDSYGQLVPSGIYLYSLKTTSGFNATKKMVFMR"
     gene            5174..6925
                     /locus_tag="PKX09_09195"
     CDS             5174..6925
                     /locus_tag="PKX09_09195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU18034.1"
                     /translation="MLLFKNSESIRDLLRNWIFRYAFILLFSTTPILLAQTFNIQGKI
                     SVKDQTPVRYASVTFINQNDTTQKYSAITDDSGYYRLTVKTGIDHLDYNLPSNIELAQ
                     NYPNPFSASTSIQYKLNIKSDVEITIYDLLGREVRKFISDNKSPGVYSVIWDGKNELG
                     QKVSTGVYIYKFQAGNEIRVGKMVFGIYDQFGAFSSFNAARFNTYFFQKKIDEAVSTV
                     SYNVVINNKAETKPRISSTQFNDCHFASDTIINFQVELFIMQFAFCYNKEWDICLNNN
                     KGTGYYNITDKVSGVDYNPTWSPDGRYIAFDHLKNYLYLYDTFNDTCIEFISSSIYSA
                     TVPYWTPDSKKLIYKYHIISYPEECHIINIDGTNDRKIDYFPDYFLSDSYTFIYEKNG
                     KIYKSNIDNSYNELVVDLNNQGEQVGTHLIQDFNPRDEVFLFCCRDSNGINMIKSCNL
                     KTKEIQVVLLGNEEYCYPRAKWSPDFLKICFMEGKEGSVDSTYDQYLSILENGISHRL
                     LRIGINDKPGGTFGFSWEDPKFSPDGKYIAYGTLFIDGDYYVILHRDLYTIEAATGEI
                     KFIDSCTYYNWNPLILH"
BASE COUNT         2163 a         1324 c         1419 g         2157 t
ORIGIN      
        1 ttaagacatt tctctttttc acaattaata caaaaaaacg agaatagaaa tacaagaata
       61 taaaaatgta aattttgatt catattaaaa cttaagaata cggataaaaa gtaatgttag
      121 tgactctatg tttaggaata aatgccattt aatccttaac acaataatag caaagaaaga
      181 tttccatata caaaaataaa caagaactgt gtctttgagc tgaagcacat atcagtcctc
      241 ggccatcgtt catagacaag tgatacatcg tcgtaccaat ttatataagc agtctaaatg
      301 aaacacttca aacaatgcca aaaaacaagc cactcactta tggattaatc aaacggagag
      361 aatgatgaat ccaacaatca taccaaaaca aagaacaggc taatgttagg gtattctttt
      421 tataaagtca atgctttttt gtttttttct gtctgatcta gggacatggt atccactttt
      481 tgcaacagcg ttgagatgat aatcggtaat gacaggcgga aaaataccga tgtggttagg
      541 attgatatta ggtggattca ggtcgtttag aatttcgatg ggtgttttca tattttaata
      601 gcggaattta cgtttatagt tgaaataaac actatatgta aaagctttac agagcagttc
      661 acggggatta tgataatctt ccatagttcg aaggactcac tataccatat catagaactc
      721 gtcttcaatt aggcggtgag cggcttcgac atctctgtta taagttttac gaccgaccgg
      781 gatttggata gattctggat tcatttgctg aacaattagt tggtagagag ttagtttatg
      841 tttctggttc caggggccaa taaactctga gccgttatca ctctgccaga ctgtttgatt
      901 taagtcaaca ccacaactaa gcaaatgaca gcccagcagg taggtaaaga tggctacatt
      961 ggtagttgtt ttctcgtaag catatcctat ataaattagc ccagcgcgaa cctcccgggc
     1021 tgtgatctgg tatttggggt aattttgcca tagataggga taatagtgcg gaatgtcgca
     1081 tagttctttc aggtcaatct gaatcttctc aaagactttt atttgggcct tcacgattct
     1141 taagtcacgt ttgacctggg ttttgtgctt acggggacgg atcaagccag cgttttttat
     1201 gatccgatga atggtagctg ggctggagtg aatgccatgc tcggctttaa tcctgatcgg
     1261 acccagataa agttgacgac gacggattgc cagaatgcgt tcttcttcct gagcgtcgat
     1321 cttataagga ctctggtttg ggcgccgatt acgatctatc aggccgacgg ttccttcttt
     1381 cagatagcgg ctcaaccatt ttctaaccgt cttgcgggtg gtaccaaatt gaacggctgc
     1441 catgctcacg ttgttattct gagcataacg gactatctta aagcgagtgt cataacaatt
     1501 tttcatttcc caataggttt cataatagtt aatttcaatc atgggctttc tctctatttg
     1561 atcgatgtga tattttagac tcgctaaaat acgcgagaaa gccctttaaa aaaagctctc
     1621 aaaggcttgt ttcgctacgc tcaacaagcc tttgaatttc aagtaactgt tctacactaa
     1681 tcctgtttat ctgtagaagg tggatacaat gtcctgatac tttgcaaccc accgcgattt
     1741 tatgctttcc aaataagaaa aatttttgtt aattcaagcc tgaatgaaga tacgcctagc
     1801 aactaatgaa gacaatcaaa gcttgctcgc gcttgcgcgc gagtcaccga tggaaagtca
     1861 cctaatcatc aatgttgacc gagcgccgga ctattttcta ctggcaaatt tacaaggcga
     1921 cgacgcccga gtatttgtag ctgaacagga tggatttatc gtaggtgcga tcggttgttg
     1981 tttgcgcgag gttttgctta ccggacaacc gactcgcatt ggttatatcg gcggaataaa
     2041 agttgccgcc gccgctcgta aaggcttgac cagcttccgc ttgatgaatg ccgtagccaa
     2101 ttatttacgg gcggttcccg tcgaaatggc tatagtaatt acgatgactg acaatgaggc
     2161 gatggcaccc attttagctg gtcgggtcgg tatgccgcct tttcacccaa ttaatcggtt
     2221 ggtaatcaat tacatttttc cactttttcg accgcaagtt actggtggct accaaattcg
     2281 atcgttccgt gaaagcgacc tcgatcatct gacacgattg ttcactgatt tttttcgaaa
     2341 ttactctttg acattcgaat ggacaccgaa acgcctttcc gctttattga accagccgaa
     2401 ttttagttcg gaaaacatac tgattgccac aaaaaatggt cgcccggttg cggcactgac
     2461 ttggtgggat caatccagtt tcaagagaac aattgtcgaa aaatatgatg gctggctaaa
     2521 aacgctagct ggcttatcga aacctttaaa aatatttccg caggcgggtg aaccgttaat
     2581 tgaactacag atccggcata tcgtttacga gaaagaacat acctcagccg cacgcgattt
     2641 aatccgatat ttaattaatg aaaaaaagcg taagtatcgc ctctttcgga tcggttttca
     2701 acaaaattca cctctcgcgg ctttgttatc cggattaccg catctgaaaa ttcatctgaa
     2761 ttgttacatc gcttcggata aaaaagacga cgaaacccgc ttgctaattt ccggtctgcg
     2821 tcaaggtcaa atctgggagg atctgtcatt acattgaaaa agaaaaaaag catttccatc
     2881 atttgtaacc tacaagcctg cggtgggaaa gcccgtcggc gctggctgga tttcttgaaa
     2941 atcgcaaccc aggctgggtt cagtatagat tttcatatca cggaatatca cgggcatgcg
     3001 acggttttag cccgcgaacg tcttgccaaa ggagccgacc ggatcatcgt tttcggtggc
     3061 gacggtactt tgaacgaagc tctcaacggc ataattgtaa aagaccgtcc ggtgaatccc
     3121 gacttgcgtt tggtttatct cacggctggc agtagttgcg atgtggctaa aatgtttccc
     3181 aatccccaaa cacttctaca acggatcgcc gatggcaagg attattcggt tgatgtgggc
     3241 aaacttgaat gtctgaacgc acgggctgaa aaggttgtgc gctatttttt ggctaattct
     3301 agcattggag taataagtcg ttccattgaa aaattcaacc agaaacgccc aacgatgaat
     3361 ttgctgaaga gaattaatat tgatctggcg gcggtcagtg ccgggattaa gaccattatc
     3421 caattcgaaa atatagacag ccgaatctcc tatgaaaatc agccctttac catccaaaaa
     3481 atcaaaaaca tcactgtttt caaatcgccc tatttcggcg gcggtatgaa ctacggtgtg
     3541 ccaactagtt ataatgatca tttcttacac gttgctacga ttgccgcaat ggatgccgtt
     3601 catacttttc gaatgattcc tttactttac tctggaacca tcttgaaaac tccgcaatct
     3661 gagtattgtc gggtacaaaa cctgagtttt gacgctcccg ggcagcatat tcccattgaa
     3721 gccgatggtg aaattgtcgg ctttccgccc tgccattatg ccattctgcc agaattaatc
     3781 catctggttg tgtaaacttc attattgaat tgaactaata tttaatgtct gaggtacaac
     3841 tgttggcggt ggtgcgggga agactatcca aaaatagcca actgaaaaaa ttttcaaact
     3901 gtaagaaaat attttgagcc acccaacggg atcgaaccgt tgacctgacg attacgaatc
     3961 gtctgctcta ccagctgagc tagggtggca aaaagcgacc aaaatttata atatttattc
     4021 ggttcttaca aaacaagcag tacgtacggg gagcattgag ttgttttatg aataaggact
     4081 tgacggataa cttattgctt attaatttag ttttagaatt atgaagtgtt taaaatgaca
     4141 gggagtcata tataagggaa ttaaatttca agacttttta caggcgttta ggaaacgttt
     4201 atctaagagg cactcccatt agatagtccg aatattgtag ggatgcgcta ggttatattg
     4261 gaaagtaagc catgaaaacc gacagaaatg aaggttggga acagagcgtt atgaccgaaa
     4321 tcgaatgcgt gactatcgcg cgaaaactgc aaaaatcacg aagagactgg ctatctcggt
     4381 ttatagtaac ttgtttctat ttaattattt taacctttaa aatctccttt acgcaaccta
     4441 taactgtcga gggctcagta ataactacca atctcttgcc tgttaaatat gcttcagtga
     4501 ctttcattaa tcagaacgac actacccaaa aatattctgc catcaccgat acctgtggtt
     4561 attattgatt gaccgtgccg tccgggatcg atcattttga ttacaagttg ccttctaata
     4621 ttgaattggc tcagaattac cccaatccct ttaatccttt gactacgatt gcttatgact
     4681 tgccgcaaga atcacaagtg atgttgacaa tttatgatat tctgggacgt gaagtaataa
     4741 gattggttga tgagagacag ccagctggaa tacaaaaagt catctgggat ggcagagata
     4801 gctatggtca attagtgccc agtgggatat atctctattc cctgaaaaca acttctggat
     4861 tcaatgcgac taaaaagatg gtctttatgc gataatacca gagccctccc gagggtttct
     4921 gtacttcggg aggatttaat tattcagtca gccaatcagt tgcctggttt acctcttacg
     4981 agaaaacaac tgttttttaa gcgaacaact atccatgact aaagaatatt aataatattt
     5041 gacaaatggc tttttacctg ataaattact ttcgaaacaa tgaaatgttt agagaatatg
     5101 atgattggat aagcaatgag gagattaaat ttcagggtta tttgaagtca taaaggaaat
     5161 gctcggcgtc aatatgttac tttttaaaaa ttcagaatct attagagatt tactaagaaa
     5221 ttggatattt cgatatgcgt ttattttact attttcaaca acacctatcc tcttagctca
     5281 gactttcaat attcaaggaa aaatctctgt aaaagaccaa acaccggtca gatacgcttc
     5341 ggtaactttt attaatcaga acgacactac tcaaaaatat tctgccatca ccgatgactc
     5401 cggatactac cgattgacag tgaagaccgg tattgatcat ttagattata atctgccttc
     5461 caacattgaa ttagctcaga attaccccaa tcctttttca gcatcaactt ctatccagta
     5521 taaactcaat atcaaatccg atgtagaaat tactatctac gacttactgg gaagggaagt
     5581 ccggaagttt atctcagata acaaatcacc cggagtatat agtgttatat gggatggtaa
     5641 gaatgaactt ggccaaaagg tatctaccgg agtttatatc tacaaatttc aggcaggcaa
     5701 tgaaattagg gtaggtaaaa tggtttttgg tatatatgac caatttggtg cattttcatc
     5761 atttaatgcc gcacgtttta atacttattt cttccaaaag aagattgatg aagcagtttc
     5821 aacagtatcc tataatgttg tgataaataa taaagctgag acaaagccta gaatctcttc
     5881 tacccaattt aatgattgtc attttgcatc ggatactatc atcaattttc aggtcgaatt
     5941 atttatcatg caatttgcgt tctgttataa taaagaatgg gatatttgcc tgaataataa
     6001 taaaggtaca ggttactata acatcacaga taaagtgagt ggcgtcgatt ataatcccac
     6061 atggtcacct gatggtaggt atattgcttt tgatcatttg aaaaattatc tatacctcta
     6121 cgataccttt aatgatacat gtatagaatt tatttcttca agcatttatt ctgcaactgt
     6181 tccttattgg actcctgaca gtaagaaatt aatctataaa tatcatatta tctcttatcc
     6241 tgaagaatgt catataatta atattgatgg cactaatgac cgtaaaattg actatttccc
     6301 ggattatttc ctatcggaca gttatacttt tatatatgaa aaaaatggca agatatataa
     6361 aagtaatatc gataattcat ataatgaact ggttgttgat ttaaataatc agggagaaca
     6421 ggtaggaact catttgattc aggattttaa tcccagagat gaggtttttt tattttgttg
     6481 tagggattct aatggaatta atatgattaa atcttgtaac ctgaaaacaa aggaaattca
     6541 ggtggtttta ttggggaatg aagaatattg ttatccgagg gcaaagtggt caccggattt
     6601 cttaaaaatc tgtttcatgg aaggaaaaga gggatctgta gattcaactt atgatcaata
     6661 tttatctatt ttggagaacg gaatatctca ccggctttta aggattggta ttaatgataa
     6721 acctggaggt acattcggtt ttagctggga agatccgaag ttttcaccag atggtaaata
     6781 cattgcgtac ggaacactat ttatagatgg tgactattat gtaatcttac acagggattt
     6841 gtataccatc gaagccgcta ccggtgaaat caaatttatc gatagttgta catactataa
     6901 ctggaaccct ttaatattac attgataaaa tatagacaga acaaattata cttatgtatt
     6961 ctctataaac ccgttgtgtg gaattaagcg agggcatgtt taatatgatt taggggttta
     7021 ttattgatag aattcaggaa ctgtagcaaa gtgaaggcag tca
//
LOCUS       DAOSMP010000075         7031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_10755_length_7031_cov_16.326978, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000075 DAOSMP010000000
VERSION     DAOSMP010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10755_length_7031_cov_16.326978"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PKX09_09200"
     CDS             <1..189
                     /locus_tag="PKX09_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18035.1"
                     /translation="SQQKINLRSFVETIRCEGTNLQIVTKVINGRTAKIKEILQTLNI
                     DGTNCRIVRQKTYLTSVE"
     gene            209..1762
                     /locus_tag="PKX09_09205"
     CDS             209..1762
                     /locus_tag="PKX09_09205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOU18036.1"
                     /translation="MEKTILISETAKETRVAILENGELVEFFVEKIDKNRLVGSIFKG
                     KVENVIEGIQAAFVDIGYESNAFLPFSEIEDSSSLAYIENISRDEEEDEQDIEDDLKP
                     TKKIHLRRGDINLQKDQEILVQVIKEPFAKKGPRVTTNISLPGRFLVLVPHAEYIGIS
                     KKILNPNEKRRLRRIAKSIKPPEFGLIIRTVAAGKDVKSLRADLDSLMDKWKQLEEKV
                     KKSKAPACVYTDMELSSTIIRDLLTPDVDKIIVDTKDLYRNILSYIKSTSPEFVNKLE
                     YYHSKNPLFEQYNIEKEFEKSLNKKVWLKSGGFIVIEHTEAMTTIDVNSGKFIGKNDQ
                     EVNSLKINLQAAKEIARQLRLRDVGGLIAIDFIDMEMEENKRRVVQELKRELQKDRAK
                     VSVANISDYGILEMTRQRTRLNLLQSVSEECPTCHGSGRITSKGSMVTKLESWFQRFK
                     LKSNERRLDLHVNPDFAHYLKDTTNKILWKIQWRNVLRIKLVEDSNVNIDEFRVYLRK
                     SGRDVTDEY"
     gene            complement(1959..3539)
                     /locus_tag="PKX09_09210"
     CDS             complement(1959..3539)
                     /locus_tag="PKX09_09210"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HOU18037.1"
                     /translation="MIKSKNFIARAFSFLLVITILLTNLQAQKCQKYYSDNGDGTFTN
                     PVIPADFPDPDVIRVGDTYYMVTTTMFIFPGVTILKSKDLVNWEYCCNAVPRFDFSPC
                     YNLDGCNRYGHGQWATSMRYHNGTFYLLFITLDEGGFLCSATKAEGPWEIKQLPKGFY
                     DPGLFFDDDGRIYVAYGYGKIYLTELDQNFAPISQDSLIYTGDLRSGLEGTHVYKIND
                     YYYLYCTYGGVDGIQVTLRSQNIYGPYEQKVAIRDTMPGVTYGVHQGALIQTQAGEWW
                     TMLFVDSGPFGRFPSLQPVTWVDGWPIVGVDGKGVVTYRKPDVGKQHPIKTLPTSDEF
                     KDKILGMQWGWNHNPDDSRWSLTQRPGYLRLVTGKVVASLPEARNMLTQRIFACYDQT
                     VPTIGITKMEVGKMKDGDVAGLAVFQNPYAFIAVKQENGIRKLIMVNNGETIAETPMN
                     GEIVFFRTIASNRTKKACFEYSYDNIEFIQLGNELLMEFSLKIFTGNKFGLFNYATKE
                     SGGYVDFDWFRTAMGLSN"
     gene            complement(3582..5672)
                     /locus_tag="PKX09_09215"
     CDS             complement(3582..5672)
                     /locus_tag="PKX09_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase"
                     /protein_id="HOU18038.1"
                     /translation="MKNISCKIEVTIGLLILLLVPFSLNAENGHDLWLRGKSIAQINV
                     ICSEKSPTLQIAKQELQQCWQGESNATISLVIKNDKAIKGDGFRMSDNKIQAKTELGI
                     LYGVYELLRRQQTGKPICEEVCNPSYERRLLNHWDNLDGTIERGYAGHSIFWRKGKDA
                     FEVTETDKQLWQEYARANASIGINGTVLNNVNASPSVLTIDYLKRVKAIADILRPYGI
                     KTYLSVNFASPAVLGKLKTSDPLDPEVIKWWKVKVQEIYSLIPDFGGFLVKANSEGQP
                     GPQDFGRTHADGANMIADALRPYDGIVMWRAFVYNPSSEDRAKQAYLEFMPFDGRFCD
                     NVIIQVKNGPVDFQPREPFSPLFGGMKKTSVMPELQITQEYLGQSVHLVFLATMWEEF
                     LQSDTYQAGKGSTIARCTDGSIFPQKHTAIAGVANIGLDTNWCGHLFAQSNWYAFGRL
                     AWNNSLTSEQIADEWVKLTFGQSAEKKDEVSSITFADWNINFLQPVKQMMINSREAAV
                     NYMMPLGLHHIFAPDHHYGPGPWWAPEGVRKDWTPPYYHQADSLGIGFNRTGTGSDAV
                     SQYHEPLQSIFSNPSTCPDIYLLWFHHLPWNYVMKSGRTLWNELCYHYDEGVNQVCEF
                     QKTWDKVQPYIDTERFIHVQNKLREQCVNAQVWKDACLLYFQQFNGLPIPYDIERPIH
                     NLEELMKSDMQKYP"
     gene            complement(6142..>7031)
                     /locus_tag="PKX09_09220"
     CDS             complement(6142..>7031)
                     /locus_tag="PKX09_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOU18039.1"
                     /translation="PAQINDIKAVIRFIRANAAKYQIDTSFIGITGFSSGGHLASLAG
                     TSGGVKQYTVGSVTFDIEGNVGQYTNYSSSVNAVVDWFGPIAISVMDSCKRSKDANSP
                     EAILIGGPIDENLDKCALANPITYIDTNDPPFLILHGDADPLVPLCQSQLFFNALQKA
                     KVPGQFVIVPGGQHGPGLFDEKYYKMMTDFFLREANITAVTHEVHNSPLNVSVSQNYP
                     NPFNPVTAITFVLPAKLFVTLVIYDSLGREVSKLVYEELPAGTYTREWNAEGLSSGTY
                     FARLQAGSYIETKKIILVK"
BASE COUNT         2169 a         1468 c         1313 g         2081 t
ORIGIN      
        1 agtcagcaaa agatcaacct gcgttcgttc gttgaaacca tccggtgcga aggtactaac
       61 cttcagatag tcactaaagt aatcaacgga cgcacagcaa agattaaaga aattttacag
      121 acgttaaata ttgatggaac taactgccgg atcgttcggc aaaaaaccta tcttacgtct
      181 gtcgaataaa aaatttagga ataagtaaat ggaaaaaaca attttaatca gcgaaacggc
      241 taaagaaacc cgggtcgcaa ttttagaaaa cggcgaactg gtcgaatttt ttgttgagaa
      301 aatagataaa aatcgcctag ttggctcaat ttttaagggc aaagtagaaa acgtaatcga
      361 agggatccaa gccgcattcg ttgatatcgg ctatgaatcc aacgcattcc taccgttttc
      421 cgagatcgag gactcgtctt cgctggcata tattgaaaac atcagccgag acgaagaaga
      481 ggatgagcaa gatatcgaag atgatttgaa accgacgaag aaaattcacc tgcgcagggg
      541 cgacatcaac ttacaaaaag atcaagaaat tctggtacaa gtcatcaagg aaccttttgc
      601 taaaaaaggg ccgcgcgtta cgaccaatat ttctctacca gggcgtttcc tggtactggt
      661 tccccatgcc gaatatatcg gcatttcaaa aaaaatcctt aatcccaatg aaaaacgtcg
      721 cctacgacgg attgccaagt ctattaaacc acctgaattc ggcttaataa ttcgcacggt
      781 tgccgcgggc aaggacgtta agtctttacg cgctgacctc gattcattga tggataaatg
      841 gaagcaactt gaagaaaaag taaaaaaaag caaagcacca gcttgcgttt ataccgatat
      901 ggaattatct tcgacgataa ttcgcgatct actgactccg gatgttgata aaatcatcgt
      961 agatactaaa gacctgtatc gcaatatctt gtcttatatc aagagcacct ctcccgaatt
     1021 cgtcaataaa ttggaatatt accactctaa gaatcctctt ttcgagcagt ataatattga
     1081 aaaggagttc gaaaaatcct taaataaaaa ggtctggcta aaaagcggtg gttttattgt
     1141 cattgaacac actgaagcga tgacgactat tgacgtcaac agcggcaaat ttatcggtaa
     1201 aaatgaccaa gaagtcaact cgcttaaaat caatttgcag gcggcaaaag aaattgcccg
     1261 acaattgcgt ctgcgcgacg tcggtggttt aatcgctatt gactttattg atatggagat
     1321 ggaagaaaat aaacggcgag tagttcagga attaaagcgc gaacttcaga aagaccgggc
     1381 taaggtatca gtcgccaata tttccgatta tggcattctc gaaatgacgc gccaacgtac
     1441 ccgcttgaat ctgctccaat ccgttagtga agaatgtccc acttgccatg gttctggtcg
     1501 aattacttcc aaaggttcta tggttaccaa gctcgaaagc tggtttcagc gcttcaaact
     1561 caagtctaat gaaagacgct tggatttaca cgtaaatccc gattttgcgc attatttgaa
     1621 agatactacc aataaaatcc tctggaaaat tcaatggcga aatgtacttc gtataaaatt
     1681 agtcgaggat agcaatgtca acattgatga atttcgggtt tatttgcgaa aatcgggacg
     1741 cgatgtgaca gacgagtatt gaaaaaataa ccacaattcg cgattacccg taatcgctta
     1801 gataatgtta tgtcattaca agggatattg gcgacttgct tgcgcccgca gagcagaata
     1861 gtacaggcag gcaataaact ccaagctaag acgaggttaa tgttgagaaa atataagtgc
     1921 tcgggattca gcccggcaac aaatgacttg ccttacgatt aattggacaa tcccatagcg
     1981 gttctgaacc aatcaaaatc aacatatccg ccagattcct tggttgcata attaaaaagt
     2041 ccgaacttat tgccagtaaa tatcttaaga ctgaattcca ttaataattc gttaccaagc
     2101 tggataaatt ctatattatc ataactgtat tcaaagcatg cttttttagt cctatttgat
     2161 gcgatagtgc ggaaaaatac tatctcgccg ttcattggag tttcggcaat agtttcgccg
     2221 ttgtttacca taatcaattt cctaatacca ttttcctgtt tgaccgcaat aaaagcatag
     2281 ggattctgaa aaacggctag ccccgcaaca tctccatctt tcatttttcc aacttccatt
     2341 ttagtaatgc cgatggttgg tacagtttga tcatagcaag caaatatacg ttgagtaagc
     2401 atattccgag cttcaggcaa actagcaacc actttgccag ttactaatct caaataaccc
     2461 ggcctctgcg ttagtgacca cctgctgtcg tctggattgt gattccagcc ccattgcatc
     2521 cccaatattt tatccttgaa ttcgtcagat gttggcaacg tttttatcgg atgctgtttg
     2581 ccaacatcag gttttctata ggtaacaacg cctttcccat caactccaac tataggccaa
     2641 ccatcaaccc aggttacagg ctgtaaagat ggaaaacggc caaagggacc gctatccaca
     2701 aaaagcatag tccaccattc gccagcttgg gtttgaataa gtgctccctg atgaactcca
     2761 tacgttactc cgggcattgt atctctaatt gccacctttt gttcgtaagg tccgtaaata
     2821 ttctgcgaac gcaatgttac ttgaatgcca tcaacaccgc cataggtaca atacaaatag
     2881 taataatcat tgattttata tacatgagta ccttcaagtc cacttcgtaa gtctccggtg
     2941 taaatcaaag aatcttggct aataggcgca aaattttgat cgagctccgt tagataaatt
     3001 ttcccatagc catacgctac ataaattctt ccatcgtcat cgaaaaataa tcctgggtca
     3061 taaaatcctt tcggcaattg ttttatttcc cacggacctt cagcttttgt tgccgaacaa
     3121 agaaacccgc cttcatcgag tgtaataaaa agtagataga aagtcccatt atgatatcgc
     3181 atacttgttg cccattgacc atgaccgtac ctgttacatc catcgagatt gtaacaagga
     3241 ctgaaatcga aacgaggaac ggcattacaa caatattccc aatttacgag gtcttttgat
     3301 tttaaaatgg ttacccccgg aaaaataaac attgtagtcg taaccatata ataggtatcg
     3361 cctactcgaa tcacatcagg atcgggaaaa tcggcaggga taactggatt agtaaaagtt
     3421 ccatccccat tatcactgta atatttttgg cacttttggg cttgtaaatt cgtcaataat
     3481 atcgttatta caagcaaaaa actaaaagca cgagcgatga aatttttaga ttttatcatt
     3541 aataattctt ccgttgagtt ttaaatttcc ttattgctga tttagggata cttctgcata
     3601 tcacttttca tcaattcttc aagattatga atgggacgtt caatgtcata aggtattggc
     3661 agtccattaa attgctggaa ataaagcaag catgcatctt tccatacctg agcgtttacg
     3721 cactgttcac gaagcttatt ctgaacatga ataaatcgtt ctgtatctat gtaaggttga
     3781 actttatccc acgttttttg aaattcgcat acctgattca ctccttcgtc gtaatggtag
     3841 catagttcat tccatagggt gcgcccactt ttcattacat aattccatgg taaatgatga
     3901 aaccacaaca aataaatgtc aggacaggtg cttggattag aaaatataga ttggagcggt
     3961 tcgtgatact gacttacggc atcactgcca gttcctgttc gattgaagcc gatgcctagg
     4021 gaatcagctt gatgatagta gggtggggtc caatcttttc ttactccctc aggtgcccac
     4081 catggacctg gaccataatg atgatcagga gcaaaaatat gatgtaatcc tagaggcatc
     4141 atataattga cggcggcttc acggctgtta atcatcattt gttttacagg ttgcaaaaaa
     4201 tttatgttcc aatcggcaaa cgttattgaa ctaacttcat cctttttctc agcactttgc
     4261 ccaaaagtca gttttaccca ttcatcagcg atttgttcac ttgtcaggga attgttccat
     4321 gctaaacgac caaaagcgta ccagttagac tgtgcaaaaa gatgaccaca ccagttagta
     4381 tctaatccta tatttgcaac tccagcgatt gctgtatgtt tttgtggaaa gatgcttcca
     4441 tcggtgcaac gagcaatggt acttcctttg cctgcttgat aagtatcact ctgtaaaaat
     4501 tcctcccaca tcgttgcaag aaataccaga tgaactgatt gcccaagata ctcctgagtg
     4561 atttgaagtt ctggcatgac agaagttttt ttcattccac caaaaagtgg actgaatggt
     4621 tctcgaggtt gaaaatcaac cggtccattt tttacctgaa taataacatt gtcgcaaaac
     4681 cggccatcga atggcataaa ttccagatac gcttgtttcg ctctatcttc acttgaagga
     4741 ttatacacaa aggcacgcca catgacaatt ccatcataag gacgaagtgc atcggcaatc
     4801 atattggctc catccgcatg cgtacgacca aaatcttgtg gccctggttg accttcacta
     4861 tttgctttaa ccaaaaaacc tccaaaatct gggatcagac tataaatttc ttgaactttg
     4921 actttccacc attttataac ttcgggatcc aatggatctg aagtttttaa ttttccaagt
     4981 acagccgggg aagcaaaatt gacggaaaga tatgtcttga ttccatacgg tcgaagaata
     5041 tcggcgatag ctttgactct tttaagataa tctattgtta aaactgaagg cgaagcattt
     5101 acgttgttga gaacagttcc gtttattcct atggatgcat tggctcgtgc atattcctgc
     5161 cataattgct tatcggtttc agtgacttca aaagcatctt ttcctttcct ccagaatatg
     5221 gaatgcccag cataaccacg ttcaatagta ccatcaagat tgtcccaatg atttaatagc
     5281 cgtcgttcgt atgagggatt gcaaacttct tcacatattg gtttacctgt ttgctgacga
     5341 cgcaataatt cataaactcc atataaaatt cctaactcag ttttggcttg aattttatta
     5401 tcactcattc taaaaccatc gcctttaata gctttatcat ttttaattac gagtgatatt
     5461 gtagcatttg attctccttg ccagcattgc tgtaattcct gttttgcaat ttgaagtgtc
     5521 ggtgattttt cggaacatat tacatttatc tgagctatgc ttttgccgcg taaccagagg
     5581 tcgtgcccat tttctgcatt gagagaaaaa gggaccaaca atagaattaa taagccaatt
     5641 gttacttcga ttttgcagga tatatttttc atttgagatt ccttttgtta gttattgctc
     5701 aatattgggg aataaaaatt ttggtcgggt accaatatct tagttctgct catctttttc
     5761 cccacaccaa aaaatgactc gctcgaagga tccgaaaaac gaaaaccatc catcgttaaa
     5821 tagtaataat ggaatcccgg cggtaatggt ttagttgtca ctgtccagac tccttcttct
     5881 ccttttacca tatcatatac gtttaagaga tcaagctgaa ctttttgagt atttggagca
     5941 tcgactcgaa agatagctct caattgtata tctactctcg gaaattcagc tccaggaata
     6001 tttgttgaag ctggttttga attcctatac aaataattgt tctgtgcaag acaagcacaa
     6061 gtcattacaa taagcaatac taaaggtact attcttttgt aaattttttt acctcatcat
     6121 aaaaatatat ttgaatgttc ctcacttcac taaaataatc ttcttcgttt cgatatacga
     6181 tccggcttgc aaacgagcaa aataagttcc gctcgaaagt ccttctgcgt tccattctcg
     6241 tgtatatgtt ccggcgggta actcttcgta aacaagcttc gacacttccc gacctagaga
     6301 atcataaatg actagggtta caaataattt tgcaggcaaa acaaatgtta tagctgtgac
     6361 tggattaaat ggattgggat aattttgaga aactgaaacg tttagtggac tattatgaac
     6421 ctcatgagtg accgcagtta tattagcttc cctcaagaaa aaatccgtca tcattttata
     6481 atatttctca tcaaataagc ctggtccatg ctgaccacca ggcactataa caaattgacc
     6541 tggcacctta gctttttgca gcgcattaaa gaacaattga ctttggcaga gaggaactaa
     6601 tggatctgca tcaccatgaa ggataagaaa aggtggatcg tttgtgtcaa tgtaggtgat
     6661 aggattcgca agtgcacatt tatcaagatt ctcatcaatt ggaccaccaa ttaaaatagc
     6721 ttcaggggaa ttggcatctt tacttctttt acaggaatcc attactgaga ttgcaatagg
     6781 tccaaaccaa tccacaacag cattgaccga actgctatag tttgtgtact gtcctacatt
     6841 accttcaatg tcaaaagtta ccgagccaac ggtatattgc ttaactcctc cagatgtgcc
     6901 agccaaagaa gccagatgtc caccagatga aaatcccgta atgccaataa acgaagtgtc
     6961 aatttgatat tttgccgcat tagctcttat aaatcttata acagctttaa tatcatttat
     7021 ttgtgcaggg a
//
LOCUS       DAOSMP010000076         6703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_11473_length_6703_cov_13.764892, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000076 DAOSMP010000000
VERSION     DAOSMP010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11473_length_6703_cov_13.764892"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PKX09_09225"
     CDS             complement(<1..424)
                     /locus_tag="PKX09_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU18040.1"
                     /translation="MNNILQKSAIFYDVFERKPGTDKLTTTYCPGCGHGNLHKLIAEA
                     LVDFGVQDRTIFISPVGCSVFAYYYFHTGNIQVAHGRAPAAATGLKRAHPEAIVISYQ
                     GDGDLAAIGTAEIIHAANRGEGITVFFVNNAIYGMTGGQ"
     gene            complement(421..1473)
                     /locus_tag="PKX09_09230"
     CDS             complement(421..1473)
                     /locus_tag="PKX09_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOU18041.1"
                     /translation="MAKQFLKGNEAVVKGAVLAGCQAYYGYPITPASEIAHAAALYLP
                     VSGGTFLQAESEIAAINMVYGAAAAGQRVMTASSGPGISLKQEGISYCAGSELPCVIV
                     DVMRGGPGLGNIAPEQGDYNQVVKGGGHGNYKCIVLAPNSVQEMCDFGMLAFDLADKY
                     RNPVYILSDGFVGQMMEPVEFPEPVKQFPEKSWAIKGTPETSDNLITSIELDPDDLER
                     HNLKLQQKYAIMEEQEVRFEDYHLSDAEIVLVGFGIISRILQTIVDNLRDVGVKVGLL
                     RPITLFPFPKKKISQLTEQAKFFLVVEMSNGQLVDDVRLAVAGRKPVYLYNRLGGNVP
                     SEKEINDELLKLMRSV"
     gene            complement(1635..1874)
                     /locus_tag="PKX09_09235"
                     /pseudo
     CDS             complement(1635..1874)
                     /locus_tag="PKX09_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
     gene            complement(1893..3077)
                     /locus_tag="PKX09_09240"
     CDS             complement(1893..3077)
                     /locus_tag="PKX09_09240"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOU18042.1"
                     /translation="MPISNYIAQSMTRSSWIRKMFETGIALKKQYGADKIYDFSLGNP
                     DLPTPEIFNRVLVEESARTGAFVHGYMQNKGYPETCAAVANLLERESGLPFTEDQVVM
                     TCGAAGAINIILKSLLDPGDEVIVVAPFFSEYEFYVDNHRGQLVISEANAELLPDPID
                     IAAKISPRTRVLLLNSPNNPTGRVYGEDIFREIGKLLTEKSAEFGRPIYLLMDEPYKR
                     IIFDGLQYRSPFRYYRHTILASSFSKDLSLAGERIGYLAISPLIEDYPALINAAAFCN
                     RVLGFINAPALMQRVIRRVLDAQVAIELYQKRRDMIYDALVKIGYEIIKPEGTFYLFP
                     KCPIDDDVKFVFALQDRLIITTPGIAFYRPGYFRISYAVPEAVIQATIPIFKQVFNEM
                     KP"
     gene            complement(3085..4548)
                     /gene="gltA"
                     /locus_tag="PKX09_09245"
     CDS             complement(3085..4548)
                     /gene="gltA"
                     /locus_tag="PKX09_09245"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOU18043.1"
                     /translation="MSSSNLSKKERLAIPRQQMPEQPPEERIHNFNEVNLGFDAETAV
                     REAQRCLDCPRPTCIDGCPVSIKIPDFIRLIVQRDFSGAARKIKEDNLLPAVCGRVCL
                     QEEQCEKTCVIRGKFGPVSIGRLERFVADYERQNGQPQVSLSTPRNGKKVAIVGSGPA
                     GLACASDLIKWGYDVTVFEALHEFGGVLIYGIPEFRLPKAILKYEIDNLRKLGVRFEA
                     NFIVGKTMTVDELFQVAGFDAVFLGVGAGLPWFMNIPGENLMGVYSANEFLTRVNLMR
                     AYEFPEADTPIKMGKQVVVVGGGNTAMDAVRTARRLGAEIATIVYRRSEVEMPARLEE
                     IKHAKEEGVIFRTLTNPIEFLPDENGWVRAARCLQMELGEPDSDGRRRPIPIPNSEFE
                     IPTDLVIMAIGNGSNPLISRTTPDLQCNKWGNIIVMGQGGRTSKRGVFAGGDIVTGGA
                     TVILAMGAGRAAARSIDEYLKTGIWVTEPDSAKENKT"
     gene            complement(4563..5447)
                     /locus_tag="PKX09_09250"
     CDS             complement(4563..5447)
                     /locus_tag="PKX09_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOU18044.1"
                     /translation="MNKIIKAEFIAPDIKRFIISAPLIARKAQPGQFVILRVWEKGER
                     IPITIVDSDLEQGTITLIIQGVGKTTRQMNLLKSGDYVHDLVGPLGRATHIENYGLAI
                     CIGGGVGTAEALPIARALKNAGNTVIAIIGARTKDLVICESEMRAFCDEVFVTTDDGS
                     YGQPGLVTDVLKTIIAGKTQPNFVLAVGPLPMMRAVSEVTRPHAIKTMVSLNSIMIDG
                     TGMCGGCRASVGGKTVFVCVDGPEFDGHQVNFEELAHRQKQFAAQEKISADILNTEPD
                     KVLHQCRLSKVISENSKF"
     gene            complement(5542..5901)
                     /locus_tag="PKX09_09255"
     CDS             complement(5542..5901)
                     /locus_tag="PKX09_09255"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOU18045.1"
                     /translation="MTNDNFKWEDLDKDGLTYLMENRKEVLRNYQKMINSLGNHLDEK
                     TRHLIMLAIQINSPSPEAIKIILPKAIRAGATRDEIIDTLILTAPVVGLSTVLKMISY
                     TLKEFEILSDVFLNPNP"
     gene            complement(5919..>6703)
                     /locus_tag="PKX09_09260"
     CDS             complement(5919..>6703)
                     /locus_tag="PKX09_09260"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOU18046.1"
                     /translation="LRRSAARLLMAEVPEDLFMRGVIETVKANLDYVPPYGTGASLYI
                     RPFQIGIGDNLGVKPAPEYLFSVFVCPVGPYFKSGFQTIKVKVEEYYDRVARHGVGQA
                     KAGGNYSASLLPLKIAHDEGYNELVYLDPVEHKYFEEISSSNIFFVFKDGSLATPKSE
                     SILESITRLSLVRVAREEFGLNMEERRISIDEIEHFAEAGACGTAAVITPIGSLGHRG
                     KLYKFYADGKEAGPITTKLYKYLTAYQVGDVPDRFGWLEEIK"
BASE COUNT         1792 a         1640 c         1483 g         1788 t
ORIGIN      
        1 tttgccctcc ggtcatgccg taaatcgcat tattgacgaa aaaaaccgtt atgccttcgc
       61 ctcggttagc ggcgtgaatt atttcggcgg tgccgattgc agccaaatca ccatcaccct
      121 gataggaaat tacaatggct tcgggatggg cgcgcttaag gccagtagcc gcggctggag
      181 cacgtccgtg ggctacctga atattgccgg tatgaaaata gtaataggca aaaacactgc
      241 agccgaccgg actgataaaa attgtgcgat cctgaacacc gaaatcaacc agtgcttcgg
      301 caatcaattt gtgtagattt ccatgtccac aaccgggaca gtaagtagtg gtaagtttat
      361 cggtaccggg cttacgctca aacacatcat aaaaaatggc tgatttctgc aatatgttat
      421 tcataccgac ctcatcagtt ttagcaattc gtcgttaatt tccttttcgg aaggcacatt
      481 gccgcccagc cgattataaa ggtacaccgg tttacgacca gcgaccgcta aacgaacatc
      541 atcaaccaac tggccattac tcatctcaac gactaagaag aatttggcct gttcagtgag
      601 ttggctgatt ttctttttag gaaagggaaa aagagtgatc ggtcttagta aaccgacctt
      661 gactccgaca tcacgtaaat tatcaacgat agtttgcaga atgcgcgata taattccgaa
      721 tcccaccaga acgatttccg catcgctgag atgataatct tcaaaacgaa cctcctgctc
      781 ttccataatg gcatattttt gctgaagttt caaattatgg cgctccaaat cgtccggatc
      841 gagttcaatc gaggtgatta gattatcgga agtctctgga gtacctttga tcgcccagga
      901 tttttcggga aactgcttta ccggctcggg aaactcgacc ggctccatca tttgaccgac
      961 aaaaccgtct gagagaatat aaaccggatt gcggtattta tcagccagat caaacgccag
     1021 catgccgaaa tcacacattt cctgaacgga gttaggcgcc agcacgatgc atttatagtt
     1081 tccgtgtccg ccacctttga caacctggtt atagtcaccc tgttcaggag cgatattacc
     1141 cagccccgga ccaccacgca taacatctac gatgacacag ggcaattccg aaccagcgca
     1201 ataggaaatg ccttcctgtt tcagactgat gccaggaccg gaagaagcag tcataacgcg
     1261 ctgaccggcg gccgccgcac cgtaaaccat attaatcgcg gctatttcac tttcggcctg
     1321 caggaatgtg ccgcctgaca ccggtagata taaagccgcc gcatgggcaa tttcactagc
     1381 gggcgtgatg ggatagccgt aataagcttg acaaccggct agcaccgctc ctttcaccac
     1441 ggcttcgttt ccttttaaaa attgtttagc catagtttaa tcctccgcta tttaatggtt
     1501 atgacctttt caggtgacac cgggcgcaga catcccgcgc agagttttac cttttcattg
     1561 ccctctctgg aaaaaacttt ctgttcgcta ccgcactccg gacagaaagc cttctcagtc
     1621 actaaatccc agcgtttgaa aactgtaatg gcacccagtt cgggacaatt atagaagcag
     1681 attccacagc cggtacagcc ttctccctta taagcggaag gatgataacc gtgagtatta
     1741 aatcggtcag tcaaatacaa aacctttggt ggacaggacg cgatacataa ttcacaaccc
     1801 ttacattctt cagagttgat gataacgtgt ccccgcgtga ccgttttttg gggtactgat
     1861 tgggttttat tcatatctgc tccattgttt aattatggtt tcatttcatt gaaaacctgt
     1921 ttgaatatag gaattgtggc ttgaatcacg gcttcgggta cagcataaga aatccgaaaa
     1981 tatcccggtc ggtagaaagc aatccccggt gttgtaatta tcagccgatc ctgcaaagcg
     2041 aaaacgaatt tgacatcgtc atcaattggg cacttaggaa agaggtaaaa agtgccttcc
     2101 ggtttgataa tttcataccc gattttcact aaagcgtcat aaatcatatc acgccgtttc
     2161 tggtataatt cgatagcgac ttgagcatcc agtactcgtc gaataactct ctgcatcaac
     2221 gctggcgcgt taataaagcc cagcacccga ttgcagaacg ccgccgcatt gattaaagcc
     2281 ggataatcct caattaatgg gctgatagct agataaccga tgcgttctcc ggctagcgaa
     2341 aggtctttgg aaaatgagga tgccaggatt gtatgccgat aatagcgaaa tggagaacga
     2401 tattggagtc cgtcaaaaat gattctctta taaggctcgt ccatcaataa ataaatcggg
     2461 cgaccaaact cggcactttt ttcggtcagc aatttcccaa tctcacgaaa aatgtcctcg
     2521 ccgtaaacac gtccggtggg attgttggga gaattcagca gtagaacacg agtacgcggc
     2581 gagattttcg cggcaatgtc aatcggatcg ggtaatagtt ccgcatttgc ttcagaaatg
     2641 actaattgcc cgcggtgatt atctacgtaa aattcatatt ccgaaaagaa aggggcgact
     2701 acaatgactt cgtcgccggg atcaagtaga gatttgagga taatgttaat tgctccagcc
     2761 gcaccgcagg tcataacgac ctgatcttcg gtaaacggca aaccactttc tcgctcgagc
     2821 aaattcgcca ccgccgcaca ggtctcagga tagcccttat tttgcatata gccatgcaca
     2881 aaagccccag tacgagctga ttcttcgacc agaacgcgat taaaaatctc cggggtgggc
     2941 aaatccggat tgccaagaga gaaatcgtaa attttatccg caccatattg ctttttcaaa
     3001 gcaattccgg tctcgaacat tttccgaatc caggacgagc gggtcataga ttgagctatg
     3061 taattggaaa tcggcatatc acctctaagt tttattttct ttggctgaat ccggctccgt
     3121 gacccagatt ccggttttga ggtattcgtc aattgaacgc gcggcggctc gaccggcgcc
     3181 catagccaga atcacagttg caccacctgt aacgatgtcg ccgccggcga agacccctcg
     3241 tttggaagtg cgccctccct ggcccattac gataatatta ccccatttat tgcattgcag
     3301 atcaggtgtt gtacgtgaga tgagcggatt tgaaccattc ccaatagcca taatgaccag
     3361 atcagttggg atttcaaatt cactattggg aatggggata ggacgtcgtc tcccatccga
     3421 atctggttcg ccgagttcca tttgcagaca gcgggcggct cttacccagc cattttcgtc
     3481 aggcagaaat tcaatcggat tggtcaaagt tcgaaaaatg acgccttctt ctttggcatg
     3541 tttaatctcc tccaggcggg ctggcatttc tacctccgag cggcggtaaa caatcgtggc
     3601 tatctcagct cctagacgtc tggcggttcg aacggcatcc atagcggtgt tcccgccgcc
     3661 gacaactacc acctgtttgc ccatttttat cggggtgtca gcttcgggaa attcataagc
     3721 gcgcatcaaa tttacgcgcg ttaaaaattc attggcggaa tagactccca tcaaattctc
     3781 accgggaata ttcataaacc agggtaaacc cgcaccgaca cccaggaaaa ccgcatcaaa
     3841 tccagcgact tgaaaaagtt catctaccgt catagttttc cctacgataa aattagcttc
     3901 aaagcgtacc cccaattttc gcagattgtc aatttcgtat ttgagaatgg ctttcggcag
     3961 acgaaattca ggaataccgt agatcaatac accaccgaat tcgtgcagag cttcgaatac
     4021 ggtgacgtcg tagccccatt tgataagatc actggcacaa gccagaccag ccggtccgga
     4081 gccgacaatg gcgacttttt tcccattgcg cggcgtacta agagaaactt gaggttgacc
     4141 gttctgccgt tcataatcgg cgacgaagcg ctccagtctg ccaatcgaga ccggaccgaa
     4201 tttgccgcga atgacgcagg ttttttcgca ttgttcctcc tgcaggcaga cgcgtccaca
     4261 gaccgcgggc agaaggttat cctctttgat tttgcgcgcc gctccactaa aatctcgctg
     4321 aacaatcaac cggataaaat cgggaatttt gatgctaacc gggcatccgt caatacaagt
     4381 cggacgggga caatcaagac aacgctgagc ctcccgtacg gcagtctcag cgtcgaagcc
     4441 gagattaact tcattgaaat tatggatacg ttcttcgggt ggttgttccg gcatttgctg
     4501 acggggaatt gctaatcgtt cttttttgct gagatttgac gatgacactg actttttcct
     4561 tttcagaact tactattttc gctaatgact tttgataatc ggcattgatg aaggacttta
     4621 tccggttcgg tatttaaaat atcggcggag attttttcct gggcggcaaa ttgtttctga
     4681 cggtgagcaa gttcttcaaa attaacctga tgaccgtcaa attccggacc atccacacag
     4741 acaaaaacag tcttgccgcc gactgacgcc cggcaaccac cacacattcc ggtaccgtca
     4801 atcattatac tattgagtga taccattgtc ttgatggcgt ggggacgggt tacttccgat
     4861 acggctcgca tcattggaag cggcccgacc gccagcacaa aattcggttg agttttacca
     4921 gctataatgg tttttaaaac atccgtaacc aaaccgggct ggccataact gccgtcgtca
     4981 gttgtgacaa atacttcgtc acaaaaagca cgcatttcag attcacaaat gaccaagtct
     5041 tttgttcggg caccaataat cgcaataacg gtattaccgg catttttcaa ggctctggca
     5101 attggcagtg cttccgctgt accaactcca ccaccaatgc aaatcgccaa gccataattt
     5161 tcgatatgag tagctcgccc gagcggtcca acaagatcgt gaacgtaatc gccgctcttc
     5221 aataagttca tctggcgggt agttttaccg actccttgaa taatcaaggt aatggtaccc
     5281 tgttcaaggt cgctgtcaac aattgtgatt ggaattctct cgcccttttc ccaaactcgt
     5341 aaaattacga attgaccagg ttgggctttg cgggcaatca gcggcgccga aatgataaat
     5401 cttttaatgt ccggtgcgat gaactctgct tttattattt tattcattgt cctctttttt
     5461 taattgattt tatcgatcgt tatatgagtg taattaacta cttaggtaat tagtttgcta
     5521 ctcagaaata ttcttcaaga tttatggatt gggattaaga aaaacgtcgg acaggatttc
     5581 aaactctttc aaagtatatg aaatcatttt taagacggta gatagaccga caaccggcgc
     5641 ggtcagaatc agagtatcaa ttatttcatc gcgggtagcg ccggctcgaa tcgcttttgg
     5701 caaaatgatt ttaatggctt ctggcgacgg gctgttgatc tggatcgcta gcattatcag
     5761 atgacgggtt ttttcatcga ggtgatttcc cagtgaatta atcattttct ggtaatttcg
     5821 taaaacttcc ttacgatttt ccatcaaata agtcagtcca tctttatcaa gatcttccca
     5881 tttgaaatta tcgtttgtca ttcatatacc ctgcttattt atttaatttc ttccagccag
     5941 ccgaatctat cggggacgtc gccaacttga taggctgtta gatatttata cagtttagtc
     6001 gtgattggac cggcttcttt gccgtcggca taaaatttat agagcttgcc gcgatgcccg
     6061 agactgccga ttggtgtgat gaccgccgcc gttccacagg cacctgcctc ggcgaagtgt
     6121 tcaatttcat caatactgat gcggcgctcc tccatattca ggccaaattc ttcgcgtgct
     6181 accctaacta atgacagacg ggtaatactt tccagaattg attccgactt gggggttgcc
     6241 agactaccat ctttaaaaac gaagaaaata tttgacgaac tgatttcttc gaaatattta
     6301 tgttcgacgg gatcgagata aacaagctca ttatagcctt catcatgagc aatttttagt
     6361 ggcaaaagcg aagccgaata attaccaccg gctttagctt gaccgacacc gtgccgggct
     6421 acccgatcat agtattcttc gactttcacc ttaatggtct ggaatccgga tttgaaatag
     6481 ggaccgaccg ggcaaacgaa aaccgaaaag agatattcag gcgccggttt gaccccgaga
     6541 ttatcgccaa tgccgatttg aaagggacga atatacaaag atgcgccagt gccatatggc
     6601 gggacataat ccaggttagc cttgaccgtt tcgataacgc ccctcataaa taaatcttcc
     6661 ggcacctcag ccatcagtag gcgggcggct gaccgccgga gac
//
LOCUS       DAOSMP010000077         6590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_11767_length_6590_cov_15.887223, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000077 DAOSMP010000000
VERSION     DAOSMP010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11767_length_6590_cov_15.887223"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /gene="amrB"
                     /locus_tag="PKX09_09265"
     CDS             complement(<1..713)
                     /gene="amrB"
                     /locus_tag="PKX09_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOU18047.1"
                     /translation="MKKGVIIMTLILSMFTDISPAQERPAYNAGAFYPANPAEITSLI
                     TAYLKNAGAKTLPQKPFGIIVPHAGYIYSGPVAAYAYREVKDQEYDAVIVLAPCHVEY
                     FPFAAIYPGDAYLTPLGKVAVDKKLAAQCVTADRLVKLATEGHLSQRFGRAEHSLEVQ
                     LPFLQIVLPNTPIVPIVIGTHDWRVLESLGNRLGDLLVQKNILIVASSDFSHYHSYSE
                     CQKIDQRAIESLKQLDAVK"
     gene            complement(716..1843)
                     /gene="amrS"
                     /locus_tag="PKX09_09270"
     CDS             complement(716..1843)
                     /gene="amrS"
                     /locus_tag="PKX09_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOU18048.1"
                     /translation="MAITRRQFCLKLGSGVAASVFVPRIIKSGWTKPATDSKPYLRAA
                     RYYTPLADRKVRCLLCPRTCLVADGKRGYCRVRENQKGSYKTLVYGRLCSINNDPIEK
                     KPLFHFLPGTMALSVATAGCNLKCKFCQNWNISQVAPEDIPFEYYSPERLVNLAQQIQ
                     SPTIAFTYTEPTISTEYILDTAKLARSKGIHSVMISNGYINSEPLSDLCQVLSAIKID
                     FKAFSDDFYRNVTDGTLQPVLDTMIQIKKSNVWLEIVNLVIPTQNDAPAQFRQMGQWI
                     SRNLGSDIPVHFTRFHPLYLMENLPPTPLRTLESAYEIARECGLRFVYVGNVPGHAAE
                     NTYCPHCNRKIIERIGYQIRTFAIKNGKCQYCQTNIPGFWS"
     assembly_gap    1928..2027
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2028..3389)
                     /locus_tag="PKX09_09275"
     CDS             complement(<2028..3389)
                     /locus_tag="PKX09_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU18049.1"
                     /translation="MKTKSLTWRKISQVFFFLLFTLTLLQATYPFKPWLPPELFLWFD
                     PLAAITTQIAGRFFDIVFLLAAIILISPLIFGRAFCGWICPMGTLIDVSDHILKPKKR
                     RDFRHLRFLKLAILTAVIVLAIFGVQYAWLLDPLPVAWRSVGVIGLAFFFLLSDSILS
                     GLISIGIFPDALLSFQDKIAEFLFPVASPNFGGLVAPLIIFLIILGLSRLSRRFWCRY
                     LCPLGALLGLVARISPLRRIVDADKCSACGICRHKCKMDAIEPDFVTTTKSECILCLN
                     CKYDCATEAISFGWRAPTQMMNKVDLSRRAFIGTSLTALAGVGFLKINKVNPRHSDRL
                     IRPPGALNEDDFLERCIRCGECVRICSTSGKCLQFSVLESGLEGFMTPLAEYRRGYCE
                     YNCNLCGQICPTKAIQPLNVSAKQKAKMGQAIFLKDRCIPYRLNENCIVCEEHCPIPD
                     KAIK"
     gene            complement(3386..4285)
                     /locus_tag="PKX09_09280"
     CDS             complement(3386..4285)
                     /locus_tag="PKX09_09280"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOU18050.1"
                     /translation="MDRRKFIKTSAFLTGGMVLRGAQILGKETTIPDLVMVKDGQPAE
                     MARKTFELLGGVSRFVTKGDYVLLKPNLSWDRAPEYAATTNPELVAEIVRLCFQAGAK
                     KVVILDNTCADARRSYDACQIPVYARQAGADVRFVRENQFVETPIPNGTNLKSWPIHQ
                     EVFNADKLISIPILKHHGIPGITSGFKNMMGLLGGQRGRIHRPFSENIVDINRVIKPN
                     LTIIDAIRVLRRNGPQGGNLNDVEQMNILLAGTDRVLVDAWAAKIFGLNPLTVEYLKL
                     AHSAGLGEIDTEQFKPVVYSFKA"
     gene            4771..>6590
                     /locus_tag="PKX09_09285"
     CDS             4771..>6590
                     /locus_tag="PKX09_09285"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOU18051.1"
                     /translation="MKISVRFIKMTRGRGCKAFFIAGLVLCLMTQSLWAKGTIRGRVF
                     DKNTKDPLSYANIMIKGTTIGTAADADGMYSITNAPDGPQTIVISYIGYVTLEVDVNI
                     PDGGTVRRDFGLEPEVLQGKEVIVTAQALGQMQAINQQLASDKIASVVSEARIQELPD
                     FNAAQALSRLPGVSTLESSGEANKVVIRGLAPQYNAVAVEGIKLASTGSTQIGVSALG
                     NTAGSISNDRSVDLSMISPYMIKSISVFKSLTPDMNANSIGGSVNMELREAPSQLHYD
                     LMWQSGYTAKTNNYGNYRAVGSISQRFFQDNLGAYLLINAEKYDRDADNMSANYRVTS
                     SVIDTSIGYRPVSVNSVTLNRHVETRKRYGGNLILDYKLPAGSIKSVNMYTRFVSDYQ
                     DYNTVLNYFDKILNFNYRGGINTTDLSVNSLELKYDFRYIQMDLKAANTHSRNDLPES
                     PYITFKTGQAMMGSVPINTVPDSLTKLWVYPGHDKVYLDYLNLFSSLYTENNDNFSSD
                     FKVPFSIGKSLLGYFKFGGQYYNQKNTNDQKTPYAEIRKSGEFQAAMVEDLESQFGIP
                     VDPSTAKFAASNFTGSSKLMQPFLDNQFGEIYWACKPDILV"
BASE COUNT         1838 a         1379 c         1502 g         1771 t
ORIGIN      
        1 aatttcacgg cgtctaactg cttgagggat tcaatcgcgc gctgatcaat tttctggcac
       61 tcgctgtacg aatgataatg cgaaaaatca ctggatgcca caatcaggat gtttttctgc
      121 acaagcaggt cgcccagtcg attgccgagt gattcgagca ctcgccagtc atgagttcca
      181 attacaatcg gtacgatcgg agtattgggt aaaacgattt gcagaaaagg cagttggact
      241 tccagactgt gttcggcgcg tccgaatctc tgacttaaat gtccttcagt cgctaatttg
      301 accagcctat cggcagttac gcactgagcg gcgagtttct tatctacggc aacttttcct
      361 aacggagtta ggtaagcatc gccgggataa atagcggcaa atgggaagta ctccacgtgg
      421 cagggcgcca aaacgattac ggcgtcatat tcctggtcct tgacttcgcg ataggcatag
      481 gccgccaccg gtccgctata gatataaccg gcatgcggaa caattatgcc gaacggtttt
      541 tgcggtaggg tttttgctcc ggcatttttc aggtaagccg ttattagaga ggtaatttca
      601 gccggattag ccgggtaaaa tgcaccagcg ttatatgccg gtcgttcctg agcaggggaa
      661 atgtcggtaa acattgataa aattagtgtc ataataatta ctcctttttt cattgtcaac
      721 tccaaaatcc ggggatattt gtttgacaat attggcattt gccattctta atcgcaaaag
      781 tgcgaatttg ataaccgatt cgttcgatga ttttgcgatt gcaatgcggg caataggtat
      841 tttctgcggc atgtcccgga acattgccga cgtaaacgaa tcgcaagccg cattcgcggg
      901 caatctcata agcggactcc agcgtgcgca agggcgttgg cggtagattt tccattaaat
      961 ataagggatg aaagcgagtg aagtgcactg gtatgtctga gccgaggttt cgcgaaatcc
     1021 actgacccat ctgccgaaac tgagcgggcg catcgttctg ggttggaatt accaggttga
     1081 caatctctaa ccagacatta ctctttttaa tctgaatcat cgtatccagc accggttgaa
     1141 gagtaccgtc agtaacatta cgataaaagt cgtcggaaaa tgctttgaaa tcaattttaa
     1201 tggctgagag cacctggcat aaatcgctaa gcggttccga gttgatgtag ccgttagaaa
     1261 tcataacgga atggatgcct ttagaccgcg ctaatttagc agtatccaag atatattcgg
     1321 ttgaaatcgt cggctcggtg taagtgaaag cgatggtagg agattggatt tgctgagcca
     1381 gattgaccag tctctcgggt gaataatatt caaacggaat gtcttcaggt gctacttgcg
     1441 aaatattcca gttctggcag aatttacatt tcaaattaca accggcggtg gcgacggaca
     1501 gagccatagt ccctggcagg aaatggaaga gcggcttctt ttcgatcgga tcgttgttaa
     1561 tcgagcaaag tcgcccgtaa accagagttt tataactgcc tttttggttt tcgcgaaccc
     1621 ggcaataacc acgtttacca tcggcaacca aacaggtgcg cgggcagagt aaacagcgga
     1681 cttttcggtc ggcaagagga gtgtaatagc gagctgcgcg gaggtaaggt ttggaatcgg
     1741 tcgctggttt agtccagccg cttttgatga tacggggaac gaaaacggaa gcggctaccc
     1801 cacttccaag tttcaagcaa aattgacggc gtgtgattgc cataacgcta catcagtttt
     1861 tttatcaatc gaacggtcac atcaactaca taataatttt ttttcaaatt acgcatcgca
     1921 ttgactcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttt gatggctttg
     2041 tcgggaatcg gacaatgttc ctcgcaaacg atgcaatttt cattcaatcg gtaaggaatg
     2101 caacggtctt tcagaaaaat tgcctgcccc attttggctt tttgctttgc cgaaacgttt
     2161 aacggctgga tggctttggt tggacaaatt tgtccgcaga gattgcaatt atattcacaa
     2221 tagccgcgcc ggtattcggc taatggtgtc ataaaacctt ccaatccact ttctaatacg
     2281 gaaaattgca aacattttcc agaagtagag caaatgcgaa cgcattcgcc gcaacggata
     2341 cagcgttcca gaaaatcatc ctcgttcaaa gcgcccgggg gacgaattaa gcggtcagaa
     2401 tgacgcgggt taactttatt aatttttaaa aagccgacgc cagccagagc agtcaaactt
     2461 gtaccgatga atgcgcgccg cgaaaggtca actttattca tcatctgggt aggtgcccgc
     2521 cagccgaagc tgatcgcctc ggtcgcacag tcatatttgc aattcaggca gagaatacat
     2581 tcggatttag tagtcgtcac aaaatcgggt tcaatggcgt ccattttaca cttatgtcgg
     2641 caaatgccac aagcgctaca tttgtcggca tcaacaatcc gacggagagg gctgattcgc
     2701 gccaccagac ccagcaaagc tcctaaagga cagaggtagc ggcaccaaaa gcgccgactc
     2761 aaacggctta agcccagtat tataagaaaa ataatcaacg gggcaaccaa tccgccgaaa
     2821 tttggactcg ctaccgggaa gagaaattcg gctattttgt cctgaaaact tagcagagca
     2881 tcgggaaaaa ttccaatact gattaaacca cttaagatcg agtcacttaa caagaagaaa
     2941 aaagccagac cgattacacc aacagagcgc caggcgaccg gcaacgggtc taatagccag
     3001 gcatattgca cgccgaaaat cgccaataca atcacggcgg tgagaattgc caatttcaaa
     3061 aagcgcagat ggcgaaaatc gcgccttttt ttcggcttca ggatatgatc actgacatct
     3121 ataagagttc ccattgggca aatccagccg cagaaagcac gtccgaaaat caatggactg
     3181 attaagataa ttgccgctaa tagaaacaca atatcgaaga agcgaccggc tatttgagta
     3241 gtaattgccg ccagtgggtc aaaccagaga aacagctccg gcggcaacca gggtttgaaa
     3301 ggataagttg cctgaagcag ggttagcgta aataacagga agaaaaagac ctgcgatatt
     3361 ttgcgccacg taagcgattt ggttttcatg ctttgaagga atagacgacc ggtttaaatt
     3421 gttcagtatc aatctcaccc aaaccagctg aatgtgccag cttcagatat tctaccgtaa
     3481 gtggattaag tccgaatatt ttcgccgccc aggcatccac caagacccga tccgttccag
     3541 ccagcaaaat gttcatttgt tcaacatcgt tcagattacc gccttgcggt ccattccgtc
     3601 gtagaacacg gatagcatca atgatggtta aattaggttt gattacccgg ttaatatcaa
     3661 cgatattttc cgaaaatggt cggtgaattc gcccccgctg accgccgagt aagcccatca
     3721 tattcttaaa accggaggta attccgggga ttccatggtg tttcagaatg gggatgctga
     3781 tcagtttatc ggcattaaaa acttcttgat gaattggcca gctttttaaa ttggttccat
     3841 tcggaatcgg ggtctcgaca aattgattct cgcgcacgaa gcgtacgtcg gcacctgcct
     3901 gccgagcata taccgggatt tggcaggcat cgtaactgcg tcgtgcatcg gcacaggtat
     3961 tatcgagaat tacgactttt ttggcaccag cttggaaaca tagacggacg atttcagcta
     4021 ccagctcggg attagttgta gcggcatact ccggagcgcg atcccagctt aagttgggtt
     4081 tcaatagaac gtagtcgcct ttggtaacga agcgggaaac accgccgaga agttcgaagg
     4141 ttttccgtgc catttcagcc ggttgaccgt ccttaaccat cactaaatcg ggaattgttg
     4201 tttctttacc gagaatctga gcacctctca gaaccattcc tccggtcagg aaggcgctgg
     4261 ttttgataaa ttttcttcta tccattatta attacctcta tttaatctaa aataatattt
     4321 atctcgcttg ctttcaagaa aaaaaaatta aaagcatttt catataaact tgtattcatg
     4381 catttctttc aatacaggat gatattcccc agccattgta acggcttaca gaagatcctg
     4441 tttgcaaagc gtttctatta tttatatcgg aaaatcagat actaaaaaat taaaaaattt
     4501 taggtgagag ttaatttttc tcttgacttc ttaaaacgtt tcaattaata ttgaacagat
     4561 gcgaattatg gattacgaga ttcgaaatca aaccagttca aagtcagaaa gcccggttct
     4621 caactttggt tcaaaagcgt aatttgacaa agaacgcagt tgcctttcat aaattatatc
     4681 tttaaaattg agaaggtaat tgataagaca tctaatagat atgtgggact atgaaaaaaa
     4741 tttacgaaac agtaaaaagg atagagtctt atgaagattt ctgtgcgttt tattaagatg
     4801 acccgaggtc gtgggtgtaa agcatttttt atcgccggct tagttctgtg cctgatgacc
     4861 caatcgctgt gggctaaggg tacaatccga gggagagtgt ttgacaaaaa taccaaagat
     4921 ccgctatcgt atgccaacat aatgattaaa ggaacaacca taggaacggc ggcggatgcc
     4981 gatggtatgt attctattac gaatgctccg gatgggccgc agacgattgt gatttcttat
     5041 attggttatg taacactcga agttgatgta aatattccag acggtggcac ggtgcggaga
     5101 gatttcggtt tggaaccgga agttctacaa ggtaaagaag ttattgtaac cgctcaggct
     5161 ttggggcaaa tgcaagccat taaccagcaa ttagcctctg ataaaattgc cagcgttgta
     5221 tccgaagctc gtattcagga attgccggac tttaatgccg ctcaagctct tagtcgcttg
     5281 ccgggcgtct cgactctgga gagttcaggc gaagccaata aagtggtcat ccggggcttg
     5341 gcaccgcaat ataacgccgt ggcagttgaa ggcataaaac tggcttcaac cggtagcact
     5401 cagataggag tttccgctct gggcaatact gctggaagta tttcgaatga tcgcagtgtt
     5461 gatctctcga tgatctcgcc ttatatgatt aaatccattt cggtttttaa gtcgcttact
     5521 ccggatatga atgccaactc cattggtggg tcggtaaata tggaattgag ggaagcgcct
     5581 tcacaactgc attatgattt gatgtggcaa tccggctaca ccgccaaaac aaacaattac
     5641 ggcaattacc gggcggtcgg ctccattagc cagcgctttt tccaggataa tctgggagca
     5701 tatctcctca ttaacgccga gaaatatgac cgtgacgccg acaatatgag cgccaactat
     5761 cgggttacca gtagtgtcat tgacacttct atcggttacc gaccggttag tgttaatagt
     5821 gtaaccctta accgacatgt tgaaacccgc aagcgttatg gtggtaattt aattttagat
     5881 tataaattgc ctgccggttc gattaagtcc gtgaatatgt atacccgctt tgtctccgac
     5941 tatcaggatt ataataccgt tttaaattat ttcgataaaa ttctcaattt caattataga
     6001 ggtggaataa atacgactga tttgtcggta aattcgctgg aattgaaata tgattttcga
     6061 tatattcaaa tggatttaaa agcggccaat actcattcaa ggaatgattt gccggaatct
     6121 ccctatataa ctttcaaaac cggccaggcg atgatgggta gtgtcccgat taatactgtt
     6181 ccggatagtt tgaccaaatt atgggtttat cctgggcatg ataaagttta tttagattac
     6241 ttgaatttgt tcagttcttt atatacggag aataatgata atttctcttc cgatttcaaa
     6301 gttcctttta gtattgggaa gtctctttta ggttatttta aattcggcgg tcagtattat
     6361 aatcagaaga atactaatga tcagaaaaca ccttatgccg aaatcaggaa aagtggtgag
     6421 tttcaggcgg cgatggtcga agatttggag agtcagtttg gtattcctgt tgatcccagt
     6481 accgccaaat ttgcggcttc caattttacc ggcagttcca aattgatgca accttttctg
     6541 gacaatcaat ttggagaaat ctactgggct tgtaagccgg atattctggt
//
LOCUS       DAOSMP010000078         6201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_12737_length_6201_cov_9.138301, whole genome shotgun sequence.
ACCESSION   DAOSMP010000078 DAOSMP010000000
VERSION     DAOSMP010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12737_length_6201_cov_9.138301"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PKX09_09290"
     CDS             complement(<1..560)
                     /locus_tag="PKX09_09290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOU18052.1"
                     /translation="MKFSILTLTYNHAPFIAQCIESVLNQTYSNWELLILDDGSTDQT
                     GSVINRYLADKRIRYYYQENQGSINMASNYNKLLSLATGEIITILDGDDFCEPELLAN
                     HYESFQEPAVVLSFCQVIVKEKGWTWIHPKIQIKPGELDIFNNNPIGNAYNLLLKQCF
                     ITTQGASVRLQALKEIGGFVSIPQLHT"
     gene            complement(591..1310)
                     /locus_tag="PKX09_09295"
     CDS             complement(591..1310)
                     /locus_tag="PKX09_09295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU18053.1"
                     /translation="MPDNEIIKIHRSKTDSSYFFNTNADLIALIPPNPGRILDIGCAS
                     GKLGEEIIKAKSPQEYIGIEIVPEIAEIANIRLSKVLIGNAESILPTIDSESFDWVIM
                     ADVLEHTINPWSVIADVSRILKPHGTVLISIPNVRNLGVLFDLVYKGRWDYTSYGIMD
                     QGHLRFFTRSTIKELLTKNKFKIKSCNSNSRNRWKKWRGKTIARILSIVIGKPSDYEE
                     FITVQWIILARKINSLGNNET"
     gene            complement(1303..2028)
                     /locus_tag="PKX09_09300"
     CDS             complement(1303..2028)
                     /locus_tag="PKX09_09300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU18054.1"
                     /translation="MAIPEIDWGAWIHWYRRLAVKLTFHLSTDIWSQSNFTKNELYKR
                     DRKLKSRIIYLGISPSNFEPIEIPVKNPKLVLTVGLIDSQRRYFVKGFDHWLELVKRN
                     PHYHFCWIGFAKQMRSNIKFLPPNCQLESPLKVTEITPFYQEASIYCQPSRIETFGKS
                     LLEAMLFGCIPVISSETALPEVAGMAGVVVSPDDWGNIDLYNIQKNYQLSPKQISEIA
                     IFNFALSKREEQIMQRIKEILNA"
     gene            complement(2310..3302)
                     /locus_tag="PKX09_09305"
     CDS             complement(2310..3302)
                     /locus_tag="PKX09_09305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU18055.1"
                     /translation="MPNWNNATDTISCLDSLLKAIDKLTISILVVDNGSEYDDINQIR
                     VFLTQKGLRFKEYKRGDSSLQPISRGNHLNINILKNQENLGYASAINTGLKLLGVDNF
                     KWLLVLNNDTEVKPDSLINLLRIAEKENQIKIWAPVITYYYNPDLIWDIGGSLTFWGS
                     RTFPGRKKARSSYPYQGFTERTFVTGCALLINSEIIKTEGFLTEKFFFGEEDYWFARL
                     MQKKQHRMAVCWEAEIKHKVSKSIKKVSPQSIVPMVYIHNLNRLIDMKMWLNSTFYPI
                     WKFLYLPYATFTLWWKNWIPLRQLIKFNKLLIKNSRYKEVVTREDFLKAKELFQ"
     gene            complement(3360..5300)
                     /gene="asnB"
                     /locus_tag="PKX09_09310"
     CDS             complement(3360..5300)
                     /gene="asnB"
                     /locus_tag="PKX09_09310"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOU18056.1"
                     /translation="MCGICGIVDPNENNHRLITEMTDLLKHRGPEKAGYFHDGNVSLG
                     HRRLAIIDLETGDQPIYNEDRTVVVIMNGEIYNFQEFKADLITRGHHFYTNSDVEILS
                     HGYEEYGLEFLAHLNGIFAFALYDIKNERLILARDHFGVKPLHYWFQNEVLLFSSEYK
                     AILVHPAMRRQVNYSALHCQVNLRYNQSDETLIKGIYRLPPASYLIYENGQVSIKKYY
                     QLPVNLNFSMTESEAIDGIRFHLQQAVRRQLISDVPLGVYLSGGMDSSALVAMMAAEK
                     IRPINTFTLGFNEATDEFDDAAVIARQFNTNHTTTALALEPLQYFPETIRHAEEPKIN
                     LLQGFHLSEFVSHSLKVVLGGLGGDELFIGYDIYRYLWAMRGLLQNRPGFCQKTLWNS
                     ISSLLFSLQNSTKILKWDEYRRGLQMLFSAGDLERCYLILRNCWDYDKRFRKEIYQPS
                     FWQMPLTPTPAYFDPIFEACQDLPPLDAIAYVEFHSKMLNDYILTEDRMSMAHSLELR
                     VPFLDKDLVEFAFTIPASLKMKNGQTKYLFRKAMAPYLPPEILQKKKWGFAVNPYDQF
                     NKDLKKVAEKVLTREFIQHQGIFNYEYLQQILNYPPDPRLRWHYNFLWIVLGLAIWER
                     MFIETENFKKAKSEINSYYKIN"
     gene            complement(5394..>6201)
                     /locus_tag="PKX09_09315"
     CDS             complement(5394..>6201)
                     /locus_tag="PKX09_09315"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOU18057.1"
                     /translation="AKPVFADIDPVSFNIDCNDLEKLITPKTKAIVVVHLHGNPCEMD
                     RIVSFARKYNLKIIEDCAQAHLSEFQGKKVGTFGDVAAFSFYPGKNLGAYGDAGALGT
                     NDETLYEKAKLLANHGRKEKYIHQTEGYNYRLDTLQAAILDVKLRHLPEWTEQRINIA
                     NTYHSLLKNLPITLPEMNPAKKHVYHIFAIATDRRQKIIEALKANQIAFGIHYPLPLH
                     LQPAYSYLHYKPGDLPNSELLSTQFLSLPMYAELSRDNIEKICQVIASVY"
BASE COUNT         1854 a         1266 c         1145 g         1936 t
ORIGIN      
        1 gtatgtaact gcgggataga aacaaagcca cctatttctt tcaaagcctg cagtcgtacc
       61 gaagcaccct gtgtagtaat aaaacattgt tttagcagta aattataagc attaccgata
      121 ggattattgt taaagatatc caattcacca ggtttaattt gaattttagg atgaatccag
      181 gtccaacctt tctctttaac tattacctgg caaaaggata aaactaccgc gggctcctga
      241 aaagattcat aatggtttgc caataattct ggttcgcaaa agtcatcacc atccaaaatt
      301 gtaattattt cgccggtcgc taggctaagc aatttattat aatttgacgc catattaata
      361 gagccttgat tttcttgata ataatatctt atccttttat ctgctaaata tcgattaatc
      421 acacttccgg tctgatcagt agatccatca tccagaatta atagttccca gttagaatat
      481 gtttggttta atacagattc gatacattgg gcaataaagg gtgcgtgatt ataggtaaga
      541 gttaaaatag aaaatttcat agaataaagt atagtctcta aaaatattta ttaggtttca
      601 ttgttaccaa gactatttat ttttcgggct aatattatcc attgcactgt aataaattct
      661 tcataatctg acggtttacc aataacaatt gaaagaattc gggcaattgt tttaccacgc
      721 cactttttcc aacggttgcg cgaattcgaa ttgcacgatt taattttaaa tttattttta
      781 gtcaataatt ctttaatagt cgagcgggta aaaaatcgca ggtgaccttg atccattatg
      841 ccatatgacg tataatccca acgccctttg taaactaggt caaataatac tcctaaattt
      901 ctaacgtttg gtatgcttat caaaactgta ccatgaggct ttaaaattcg ggaaacatct
      961 gctattactg accatggatt aatagtatgt tctaaaacat ctgccattat tacccaatca
     1021 aatgattcgg aatctatagt tggcagaata gattctgcat taccgatcag tactttagaa
     1081 agccgaatat tggcaatttc cgcaatttct gggactattt cgatgccgat atattcttga
     1141 ggtgatttcg ccttaattat ttcttcccct aattttccag atgcgcaccc tatatccaaa
     1201 attcgccctg gattgggtgg gattaaggct atcaagtcgg catttgtatt aaaaaagtag
     1261 cttgaatcag tctttgacct atgaattttg ataatctcgt tatcaggcat ttaagatctc
     1321 cttaattctt tgcattattt gctcttctct ttttgatagt gcaaaattaa atatagcgat
     1381 ttcactaatt tgtttgggcg atagctgata atttttctga atattatata aatctatatt
     1441 tccccaatca tcgggagata ctacaacccc agccataccg gcaacttccg gcaaggctgt
     1501 ttctgagcta attaccggaa tacatccaaa caacatcgct tctagtaatg acttaccgaa
     1561 agtttctatc ctcgaaggtt ggcaatagat acttgcctct tgataaaacg gggttatctc
     1621 cgtaacctta agtggtgact ctaattggca gtttggtggc agaaatttaa tattcgaacg
     1681 catttgttta gcaaaaccaa tccagcaaaa gtgataatgt gggtttctct tcactagttc
     1741 cagccaatgg tcaaatcctt tgacaaaata tcgcctttgg ctatcaatca atccaaccgt
     1801 caaaactaat ttcgggtttt ttaccggtat ttctataggc tcaaaattag atgggctaat
     1861 acctaaatat ataatccttg atttaagttt cctatcacgt ttgtaaagct cattttttgt
     1921 aaaattactt tggctccaaa tatcagtaga tagatggaaa gtcagtttga cagctaaccg
     1981 ccgataccag tgaatccatg ccccccaatc aatttctggt atagccaaaa catcaaaacc
     2041 acccgcaatg gtgataaagg gttttttaaa taaccgtgcg agaatagctg gtaatgccga
     2101 atgataatca gcgaaccaag taactattaa ttgatttcta aaagtctcat agacgcttct
     2161 gataataaac cttacggttt tacctatatc ttttccgaag ctattagaat aaattgcctt
     2221 aacctgtcca attttagaga atatatcgat atcatcctgt acgaagggac taggaattgg
     2281 agcaatgatt agtattttta attttcttac tattgaaata actccttcgc ttttaagaaa
     2341 tcttctcttg taactacttc tttatatcgt gaatttttta ttaataattt attaaactta
     2401 attaactgtc tcaaaggaat ccaatttttc caccacagtg taaaagtagc gtagggaagg
     2461 tagagaaatt tccaaatagg ataaaaagta gaattaagcc acattttcat atctattaaa
     2521 cggttcaaat tgtggatata aaccattgga acaatagact ggggagagac ttttttaatt
     2581 gatttagaaa ccttatgctt aatttctgct tcccaacaca ccgccatgcg atgctgtttt
     2641 ttctgcatca atctcgcaaa ccagtaatcc tcttcgccaa agaaaaattt ttctgtcaaa
     2701 aatccctcgg ttttaattat ttctgaattt atcaataaag cacagcccgt tacaaatgtc
     2761 ctttctgtaa aaccctgata tggataactt gaacgtgcct ttttcctgcc tggaaaagta
     2821 cgactccccc agaaagttaa ggaaccacca atgtcccaaa ttaaatctgg gttataataa
     2881 taggttataa caggagccca aatcttaatc tggttttcct tctctgctat acggagcaag
     2941 ttaattaaag aatccggctt aacctcagta tcattattta agactaataa ccatttaaaa
     3001 ttatctaccc caaggagttt cagtccggta ttaatggctg acgcatagcc tagattttct
     3061 tgatttttaa gaatattaat gtttaaatgg tttcccctag atattggctg taaactagaa
     3121 tcccctcgct tatattcttt aaatcttagt cctttctgag tgagaaagac cctaatttga
     3181 ttaatatcat cgtattcgga accgttatcc acaaccaaaa tagagattgt caatttgtct
     3241 attgctttta acagtgagtc aagacagctt atcgtatcag tagcattatt ccagtttgga
     3301 attattattg ctaaatcgaa ttggttctgg acggtagtaa ttattcagct ccgatctaat
     3361 taatttatct tgtaataaga atttatttca gatttagcct ttttgaaatt ttccgtctcg
     3421 ataaacatcc tctcccagat tgccagccct aagacgatcc acaggaaatt ataatgccag
     3481 cgcaggcgcg ggtcaggcgg gtaattcaga atctgctgaa ggtattcgta attgaaaatt
     3541 ccctgatgtt ggatgaattc acgcgtcagc actttttcag ccactttttt caaatccttg
     3601 ttgaattgat catagggatt gacggcaaaa ccccattttt tcttctgcaa aatttccggt
     3661 ggtaaatagg gcgccatcgc tttccggaaa agatatttcg tttgtccgtt cttcattttt
     3721 aaactggctg gaatcgtaaa ggcaaactcg accagatcct tatcaaggaa cggcactcgc
     3781 aattccagcg aatgcgccat tgacatccga tcctcagtca gaatataatc attcagcatt
     3841 ttggaatgaa attcgacata ggcaatcgca tccagcggcg gtaaatcttg acacgcctcg
     3901 aaaatcggat cgaaataagc aggcgtcggc gtcaagggca tctgccagaa ggacggctgg
     3961 taaatttcct tcctaaatct tttatcgtaa tcccagcaat tgcgcagaat caggtaacat
     4021 ctttccagat cgccagccga aaacagcatt tgcaagcccc gtcggtattc gtcccacttg
     4081 agaatcttag ttgaattctg caaactgaaa agcagagagc taattgaatt ccaaagcgtt
     4141 ttttggcaga accccggacg gttttgcaat aaccctcgca tcgcccataa atagcgataa
     4201 atatcataac caatgaaaag ttcgtcgccc cctaaaccac cgagcacgac tttcaaacta
     4261 tgcgaaacaa attccgataa atgaaaaccc tgcaggagat tgatcttcgg ctcctcggcg
     4321 tgcctgattg tttccggaaa atactgtaat ggttccaacg ccagtgctgt agttgtatgg
     4381 ttcgtgttaa attgccgggc aattaccgcc gcatcatcga attcgtcggt tgcctcattg
     4441 aagccgagcg tgaaagtatt aattgggcgg attttttccg ccgccatcat cgccactaag
     4501 gccgacgaat ccattcctcc ggaaagataa acacccagcg gcacatcgct gattagctgg
     4561 cgtctcacgg cttgttgtaa gtgaaaacga atgccgtcaa tggcttcgga ttcagtcata
     4621 ctgaaattaa gatttaccgg caactgataa tatttcttga tgctcacttg cccgttttcg
     4681 taaatcaaat aactggcggg cggcagacga taaatccctt tgatcaatgt ttcatcactt
     4741 tgattgtaac gtagattgac ctggcagtgc agagcggaat aattcacctg acgtcgcatt
     4801 gccggatgca ccagaatggc tttatattcc gaactaaaca acagcacttc gttctgaaac
     4861 caatagtgga ggggtttgac tccaaaatga tcacgcgcca aaatgagtcg ttcgttttta
     4921 atatcatata acgcaaaggc aaagatacca ttgagatgcg cgagaaattc cagtccatat
     4981 tcctcgtaac cgtgactcaa aatctcgacg tccgagttgg tataaaaatg gtgaccgcgg
     5041 gtgattaaat cagccttgaa ttcctgaaaa ttgtaaatct caccgttcat tatgacgact
     5101 accgtgcggt cttcattata aatcggctgg tcgccagttt ctaaatcaat gattgctaga
     5161 cgtcggtgtc cgagactgac attaccgtcg tggaagtacc cggctttttc cggaccgcga
     5221 tgctttaata aatcagtcat ctcggtaatc agccgatggt tattttcatt cggatcaact
     5281 attccgcaaa tgccgcacat gatttataat tttgctaaaa gttcatcgta aaattgttat
     5341 catattccgt ccggcaataa ggaaatagcc gagcgaaaac aattctttca aaattaataa
     5401 acactcgcga tcacctggca tattttctcg atattatccc ttgacagttc agcatacatt
     5461 ggcaaagata gaaactgggt ggatagcagt tcggaatttg gtaaatcgcc tggtttataa
     5521 tgcaaataac tataagccgg ttgtaaatgc agcggtaatg gataatgaat accgaaagct
     5581 atttgattgg cttttaaagc ctcaattatt ttttggcgcc gatcagtagc gatggcaaat
     5641 atatgataga catgtttttt ggcggggttc atttccggta aggtgattgg taggtttttt
     5701 aataacgaat ggtaagtatt ggcaatgttg attcgttgct cagtccattc tggcaggtgg
     5761 cggagtttga catccagaat agccgcctgc aaagtatcga gtcggtagtt atatccctcg
     5821 gtctggtgga tatatttttc tttgcgtcca tgattagcca ggagcttcgc cttttcgtaa
     5881 agagtttcat cgttagtacc caatgctccg gcatcaccat aagcacctaa attttttccg
     5941 gggtaaaagc taaaagcggc tacatcgcca aaagtaccga cttttttacc ttgaaattct
     6001 gacagatggg cttgagcgca atcttcgatg attttcaaat tatatttcct ggcaaagctg
     6061 acaattcggt ccatttcgca gggattaccg tgcagatgaa ccacaacaat ggctttagtc
     6121 ttaggcgtta tcagcttttc tagatcgttg caatcaatgt taaacgaaac cggatcaata
     6181 tcggcaaaaa caggcttagc a
//
LOCUS       DAOSMP010000079         5955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_13484_length_5955_cov_14.461864, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000079 DAOSMP010000000
VERSION     DAOSMP010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13484_length_5955_cov_14.461864"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1184
                     /locus_tag="PKX09_09320"
     CDS             156..1184
                     /locus_tag="PKX09_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU18058.1"
                     /translation="MTRITIEEVAKQANVSKGTVSAVINGKNSVNPATREHILKVMKD
                     LNFRPRGMARNLKNGDQVRCIGIIIKDLNYPFYTSIAAGAREYANSKGYSVIVASSEN
                     DHECEKKFTHLFSIKDIKGAIIAPIVEGEAEIDHLFKLKRINYPFVLLEDVKGIQANV
                     VAIDNLSAIKTAVKYLIDNGHTKIVHFAGPPQSSHTQERIEGFRHAFSESPLIFNKNL
                     IISIGSRHEEAYGNTMNYFKNRSREDYPTAIVCFNDQQALAVMTALKELNIRIPEDIS
                     IIGNDDIMYAKIYPVPLTTIRAPQREIGYKAAEILIRNIESSTAIPNEKIVLGTDLVI
                     RESSRVLN"
     gene            complement(1367..>3345)
                     /locus_tag="PKX09_09325"
     CDS             complement(1367..>3345)
                     /locus_tag="PKX09_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOU18059.1"
                     /translation="AIRPANLTTTWTAYTKDFTITDNETVIRAPIHFSYSANVDNEIY
                     IDNFRIVYLDEIKKPVVVEAESGVVGSDFSILEEDSITYVTIQTNLAAYNPGSSARVI
                     SYEVTFPDTGSYDLFVRLRTGADTYNDDSFFYGKGFGEKDPSVDSVWVCINGLAAAGF
                     SGPNEIVRDAGGLGSGVWKWVNLSRNAYQGSAESLVTFIIDDPDSLTQTFQIGAREDG
                     LDIDKIAFGKSYLYYTVGNLDNREPGSTKLPGEVWEGPPLAHKQPKFVGNIYSSSQIS
                     NFEAYWNQVTPENAGKWGSVEGTRDVMNWSGLDAAYKLAKDNGFPFHFHVLVWGAQQP
                     SWINDLTPEEQLEEIREWFEAVAERYPDIDFLEVVNEPLPGHNPPDGTSGRANYKEAL
                     GGNGETGWDWVIKAFEMAREIFPEGTRLMINDYNIISSSSSTSQYLNLIRLLQERNLI
                     DIIGEQGHAFTTTAAVATMKKNLDSLASTGLPIQITELDIDGPSDAVQLQSYQRIFPT
                     LYEHPGVEGITLWGWRRGLWRDEQGAYLINQDGSERPALVWLRTYLDTLNLNVMVKED
                     IINVLDEFSLGQNYPNPFNPTTTINYHLPTTSHLTIKMYNLLGKEVATLVDDVKQAGS
                     HSVTFDGSGLAGGVYLYQLKTANYTDTKKCLFLK"
     assembly_gap    3346..3445
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3490..>5955)
                     /locus_tag="PKX09_09330"
     CDS             complement(3490..>5955)
                     /locus_tag="PKX09_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOU18060.1"
                     /translation="SEGHQGGLEIIQHENRVATNGEIRINATPGQWQPTAKLGEGLQK
                     RGVSPQTLAIESRKVDRKKNEISIPCSFPDESLNRKGFNPIIYPDLHTKYTIRVRAKG
                     QSIFVTVDFDEPIPPDWVGKIGFNLELFPGNLYGKHYYMDGKSGIFQRQPEGEAVLND
                     AGEAEAPALAEGQVLVVAPESDLFRMKIESKGSKLVLLDGSLQHNNGWFVVRSLVPSG
                     VTKNAIEWVITPNVIPNYKYEPVIHISQVGYHPNQKKIAYIELDKRETNIQNVTLYKI
                     TDNGEYKVITDRKPKEWGQYLRYKYALLDFSDIKESGIYVVKYGNKVSNSFRIDPAIY
                     KHNVWQPTLDYFLPVQMCHMRVNQKYRVWHGACHMDDALMMPLNIRHFDGYNNEGMTS
                     TLTQYKPLEHVPGLNVGGWHDAGDYDLRVESQAQAVLTLALAYEEFGIEWDNTFVDQE
                     NHHVEIHQPDGKPDILQQVEHGVLTILAGYRQFGRLYRGIICPTLRQYVLLGDGSTMT
                     DGKVFDDPEAKAKAANIDELWYKKVANRYSKIFDPELNAHEIQVYIPDLDDRLVFTDE
                     KADLQLAGATGLAAAARVLKGYNDELAAECLTVAEALYEANKNQSSVSEFMRDNARVN
                     ALCELIITTNSQKYKDELCSMKDEIVKKFQLSATAIARVMPYIDCEEFKTVIYTAAKE
                     LKPELDKIMNETPFGCPTRTPEYLGYSAYFLHKAWPDVFSPDHLYSVVNFLLGCRPGS
                     TTNSLVSGVGANSPTIAYGANRADWSYIPGGTFWAAVNLVRPDFPEDKDWPYLWQERE
                     YIIGGACTYMFIVMAANHLLTGN"
BASE COUNT         1620 a         1346 c         1165 g         1724 t
ORIGIN      
        1 ttttttggtg attggaatcg gtcaggtatg gtattacggc tattttttct ttacaactat
       61 aaacgaagtg tcagtattgg cggcaacgaa ttttttctta aaccaaaaaa tttggactca
      121 cttatggctc gtatgattat gaaccggagt tttctatgac gagaattaca attgaagaag
      181 ttgctaagca ggctaacgtt tctaaaggaa cggtatctgc tgtaataaat ggtaagaatt
      241 ctgtcaatcc cgctacgcga gaacatattc ttaaggtaat gaaagacctt aactttcgtc
      301 cccgaggtat ggctcggaat ctgaaaaacg gtgatcaagt tcgctgtatc ggtataatca
      361 ttaaagactt gaattaccca ttttacactt cgattgcggc gggcgccaga gaatatgcta
      421 atagtaaagg gtattcggtg attgtcgcta gttcggaaaa tgaccacgaa tgcgagaaaa
      481 aatttacgca cttgttttct atcaaagaca ttaaaggcgc gataatagcg ccaatcgtcg
      541 aaggtgaagc ggaaattgat catcttttca aattaaaaag aatcaactat ccctttgttc
      601 tacttgaaga tgtcaaaggt attcaggcta atgtggttgc cattgacaac ctatccgcca
      661 ttaaaacggc agtcaaatat ttaattgata atgggcacac caagattgtc cattttgcgg
      721 gaccgccaca gtcctcacac actcaggagc ggattgaggg ctttcgtcat gcttttagtg
      781 aaagcccgct tattttcaat aaaaatttga tcatatccat aggttcgcgt catgaagaag
      841 cctatggtaa tactatgaat tattttaaga accgaagccg ggaagattat ccaaccgcaa
      901 tagtttgttt caatgatcag caagctctgg cggttatgac tgctcttaag gaattgaata
      961 tcagaattcc ggaagatatt tccataatcg gcaatgatga tattatgtat gctaaaatct
     1021 atccggtgcc tctgacaacg attcgggcac ctcagcgcga aatcggttat aaagccgccg
     1081 aaatcctgat tcgcaatatc gagtcctcaa ccgctatccc caatgagaaa attgttctgg
     1141 gaaccgatct ggttatccgc gaatcatccc gggtgctaaa ctaagaatct tattttacta
     1201 ctgcccaaag caagcagttc taatcaattt tccctaccag ttaccaactt tgttttcatt
     1261 tataacaacc ggtctgatct gcgttttaac agaccgaccg gttgattata ataatccacc
     1321 tgatttatct ggtcaggtga actgtttgag aacgactatt atttttctac ttcaagaaca
     1381 ggcatttttt tgtgtcggta tagttagccg tttttaactg atacagatag actccgccgg
     1441 ctaatccact cccatcaaac gtgaccgaat gactgccggc ttgtttgaca tcatcaacca
     1501 aagtggcaac ttcttttccg agcagattat acattttgat agtcaagtga ctagtagtag
     1561 gtaaatgata attgatggtg gttgtcggat tgaagggatt gggataattt tgacctaagc
     1621 taaattcatc gagaacgtta ataatgtctt ctttgaccat aacgttgagg tttaaagtat
     1681 ccagataggt tcttaaccat accaaagccg gtctttccga accgtcctga ttaatcaggt
     1741 aagctccctg ttcgtctctc caaagacccc gtcgccagcc ccacaaggta attccttcta
     1801 ctccagggtg ctcataaaga gtcgggaaaa ttctttgata actttgtaat tggaccgcat
     1861 cagaaggtcc atcaatatct aattcagtaa tttgaattgg taatccggta gaagcaagag
     1921 aatcgaggtt ttttttcatt gtggctacag cggcagttgt agtaaatgca tgcccttgtt
     1981 cgccaattat atcgattaaa tttctctctt ggagcaatct aataagatta agatattggg
     2041 atgtactgct tgagctactg attatattat aatcatttat catcagtcta gtcccttctg
     2101 ggaaaatctc acgtgccatt tcaaatgcct taatcaccca atcccaaccg gtttcaccgt
     2161 taccgcccaa agcctcctta tagttagctc gtccactagt gccatccggc ggattatgac
     2221 caggcaatgg ttcattgacc acctccagaa aatcaatatc gggatatctt tcggcaacgg
     2281 cttcgaacca ttctctaatt tcttcaagtt gctcttcagg tgttaaatcg tttatccagc
     2341 taggttgctg agcaccccaa acgagcacgt gaaaatgaaa cgggaaacca ttatccttgg
     2401 caagtttata ggcagcatct aatccgctcc agttcataac gtcacgagtg ccctccacac
     2461 taccccattt gccggcattt tctggagtaa cttggttcca gtaagcttcg aaattactta
     2521 tctgcgaaga actgtaaata tttccaacaa attttggttg tttatgagcc agcggtggac
     2581 cttcccatac ttcacctggc agttttgttg aacctggttc tctattatca aggtttccta
     2641 ccgtataata tagatatgac ttaccaaagg caatcttgtc aatatccaat ccatcttcgc
     2701 gtgcgccaat ttgaaaagtt tgggttaaac tatctggatc gtcaataata aaagtaacaa
     2761 ggctctcagc ggaaccctga tatgcatttc gtgaaagatt aacccatttc caaaccccag
     2821 aaccaagacc gcctgcgtca cgaacaattt cattcggtcc cgaaaatccg gcagccgcaa
     2881 gtccatttat acaaacccaa acagaatcaa ctgagggatc tttttctcca aatcctttgc
     2941 catagaagaa actgtcatca ttatatgtat ctgctcccgt tcgtaaacgg acaaaaaggt
     3001 catagctccc ggtatctggg aaagttactt cgtaggatat tacccgggca ctactgccag
     3061 gattgtaagc tgctaaattc gtttgaatag ttacataagt aatcgaatcc tcctctaata
     3121 tactgaaatc acttcccaca actccagatt cggcttcgac aactactggc tttttgatct
     3181 catcaagata tactatccga aaattatcaa tataaatctc gttatccaca ttcgccgaat
     3241 agctgaaatg aatcggcgct ctgatgacgg tttcattatc cgttatcgta aaatcttttg
     3301 tataggctgt ccaggtagtg gtaagatttg ccgggcggat agccgnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnttttt tcgttattct tatcctacat ttttaaatat
     3481 aacatttaat caattacctg tcaatagatg atttgccgcc atcacaataa acatataggt
     3541 acaggcacca ccaatgatat attcgcgttc ctgccataga tacggccaat ctttatcctc
     3601 ggggaaatct gggcgaacca gattaacagc cgcccaaaat gtaccaccgg gaatatagct
     3661 ccaatcggcg cgattcgctc cataggcgat tgtcggtgaa tttgcgccga ctccagaaac
     3721 cagactattg gtagtgctac ccggtcggca tccgagtaag aaattgacta ccgagtataa
     3781 atgatccggt gagaacacat ccggccaggc tttatgcaga aaataggcgg aatatcccag
     3841 atattctggg gtgcgggttg ggcaaccaaa cggagtttca ttcattattt tgtccaattc
     3901 cggctttaat tctttagccg ccgtatagat aaccgtttta aattcttcac aatcaatata
     3961 aggcataact cgtgcaattg ccgtagcgct tagttgaaat ttttttacaa tttcatcctt
     4021 catactgcac aattcgtcct tgtatttctg ggaattggtc gttatgatca gttcacataa
     4081 agcgttcacg cgggcgttat ctctcataaa ttcactaacg ctgctctgat tcttgttggc
     4141 ttcatacagc gcttcggcga cggtgaggca ttctgccgcc agttcatcgt tatacccttt
     4201 caagactcga gcggcggccg ctaaaccggt tgctcccgcg agttgtaaat ccgccttttc
     4261 atcggtaaaa accagacggt catctaaatc gggaatataa acttgaattt cgtgggcatt
     4321 caattccgga tcgaaaattt tagagtagcg attagctact tttttatacc acaattcgtc
     4381 aatattggcg gctttggctt tggcttccgg atcgtcgaaa actttgccgt ccgtcatcgt
     4441 ggacccgtca ccaagcaaga cgtactggcg taaagtagga cagatgatcc cgcgataaag
     4501 gcgcccaaac tgccgatagc ccgccagaat cgtcagcaca ccatgctcta cctgttgcag
     4561 gatatcaggc ttgccatccg gctgatgaat ctcgacgtga tgattttcct gatcaacaaa
     4621 agtattatcc cattcgattc cgaactcttc ataagccagc gccagggtca gaaccgcctg
     4681 agcttgcgat tccactctga gatcgtaatc accggcatca tgccaaccgc cgacgttcaa
     4741 acccggcaca tgctctaaag gcttatattg cgtgagagtt gaggtcattc cttcattatt
     4801 ataaccatcg aagtgtctaa tattcagcgg catcatcagg gcatcatcca tgtgacaagc
     4861 gccatgccag acccggtatt tttggttcac gcgcatatgg cacatttgaa ctggtaaaaa
     4921 ataatccagc gttggttgcc agacattgtg cttgtaaatg gcgggatcaa tccggaatga
     4981 gttactgacc ttattgccat atttcaccac atagatacca gattccttga tatcggaaaa
     5041 atccagtaat gcatatttat atcggagata ttgtccccat tcctttggtt tccggtcggt
     5101 tatcacctta tattcaccat tgtcggtaat tttatacaat gtcacatttt gaatattcgt
     5161 ttcgcgttta tccaattcga tgtaggcgat cttcttctga ttaggatgat aaccaacctg
     5221 agaaatatga ataaccggtt catatttata gttgggaatg acattagggg ttattaccca
     5281 ttcaattgca tttttagtaa cgccgcttgg aaccagcgaa cgcaccacaa accagccgtt
     5341 attatgttgc aaactaccat caagcaaaac gagtttactc cccttacttt cgattttcat
     5401 acgaaagaga tcgctttccg gcgcaacgac caacacctga ccttcagcta aagctggcgc
     5461 ttcggcttca ccggcatcgt taagcaccgc ttcgccttcc ggctggcgtt gaaaaatacc
     5521 ggatttcccg tccatataat agtgtttacc gtacaaattg cccggaaata attccagatt
     5581 gaatccaatt ttccccaccc agtctggcgg gatcggttcg tcaaagtcaa ccgttacgaa
     5641 aattgactga cctttcgctc ttactctaat agtatatttg gtgtgaagat caggataaat
     5701 gataggattg aatcctttcc gattcaaaga ttcatccgga aaactgcaag ggatgctgat
     5761 ttcattcttt ttccggtcaa ccttacgact ttcaatcgcc agagtctggg gactgacgcc
     5821 tcgtttttgt aagccttcac ccaatttagc ggtcggttgc cattgcccgg gtgtggcgtt
     5881 tatccgaatt tctccgttag tcgccacgcg attttcatgc tgaataattt ccagaccgcc
     5941 ctgatgtcct tcgga
//
LOCUS       DAOSMP010000080         5911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_13618_length_5911_cov_22.000171, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000080 DAOSMP010000000
VERSION     DAOSMP010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13618_length_5911_cov_22.000171"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2021
                     /locus_tag="PKX09_09335"
     CDS             <1..2021
                     /locus_tag="PKX09_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU18061.1"
                     /translation="SGTIEHKSDGATTTSLNVSGAFLANEPTAASVISPDSVTGKTAT
                     LSFSGGDGARRVLTIRPGTEIAWLPEDGKPVRGVSSIFTEAIDQGDGTRIVYDGEDTT
                     VTVTGLTGNTTYTAAIFEYNVATGNTYNYLTTQYGIFTFTTAIVPELSVSPIVLNFGR
                     VLISHKLIRSYTLTGNYLTANNSTRLIAPPGFDLSLAPDTGFVPLLPIASTTLSFDTT
                     VYVRFTPTEPHTYSGFITHTLGNDTVTLAVSGRGVYSLVPTAEPVGFATLNGGASGGQ
                     GGDSVIVTTAEHFYELMHARENKSTAPLIVHISGTLSGYSSKIAIKRTANISIVGLDS
                     DAGLNGFGIKIAECNNIIVRNLTFADCHVDEKDALEIDSCQNVWVDHCTFSDSPDSDP
                     SGSNHDGLLDIKNGSRNITVSYNYFTNHRQTCLLGHTESQPTDTVMTVTYYRNWFDGT
                     YSRHPRIRFARAHIVNNLYTDIGSYGVGVTCNAQVLVEANHFENTPTPILISQVNDPN
                     RTLSGDPAGYVKALDNLTINSGAIVENLSNYHFDPNDYYSYDVAPINMVKTLVMENAG
                     AGRLDTNQLVTRKPDIPSPNRFQLLPNYPNPFNAVTTIGFQIQVAGKVSLKAYDLIGR
                     EVATIFAGEKPAGYYRFTFDANDLPSGIYLYQLKSGNFRQTRKMTILK"
     gene            2075..>3687
                     /locus_tag="PKX09_09340"
     CDS             2075..>3687
                     /locus_tag="PKX09_09340"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HOU18062.1"
                     /translation="MKKMKRRTFLKSMLLCELILLASFGLLASNLWASGNEVHRYLSD
                     LPFEMPEMALPEFPDRVFNVTDFGAIGNGLTMNTTAINNAIKACSQSGGGTVIIPRGL
                     WLTGPLELADNVNLHLQNGALVIFSPNRSDYPIINLPGKGFTTQPLIYGEKLENIAIT
                     GDGVFDGCGENWRPVKKFKMTASQWQNLLKSGGVVNRSGDIWWPSVEAMQGEHYRDSL
                     LASKPKKQLTAEDFLPARDFLRPELVLLNNCKRVLVDGPTFKNSPKWAFHTQRCENII
                     IRNVKINNEWWAQNGDGIDISSCKNVLIYKCTVTAGDDAICLKSSKTKGQTEPSLQNV
                     VVRDCIVYHGHGGFVIGSNTDGGMQNIFVDNCIFIGTDIGLRFKSARDRGGLVENVII
                     RNIYMKDIVNEAILFNTYYENIGREEKTYPVTEKTPIFTNFRLDSIFCFSAQQAIGVV
                     GLPEMPISQIVIRNSILSADVGVTAENARDFILENVNIIPKTEPILHLTQCANFHLDT
                     IGFPATISTFMTLSGKNTQAIHIKNTALSS"
     assembly_gap    3688..3787
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3925..4884
                     /locus_tag="PKX09_09345"
     CDS             3925..4884
                     /locus_tag="PKX09_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOU18063.1"
                     /translation="MPKILITGALGQIGSELVPALCQRYGSRNIIASDIRPASDQTIL
                     SAATFETLDVTNFNQIAEIVNKYQITTIYHLAALLSAVAEAHPQKAWHINMNGLYNML
                     EVARQYHCAVFMPSSIAAFGTSTPRYMTPQDTIQRPDTIYGVTKVAGELLCDYYFKRF
                     GVDTRGVRYPGIISSKTPPGGGTTDYAVHIFYAAIKQKRYTCFLKPETYLDMMYIPDA
                     IKAAINLMEADPARLQHRNAFNVTAMSIDPEMLAAEIRKHIPDFTMGYAIDEVRQRIA
                     DSWPSHIDDSAARAEWDWRPDYNLETMTTDMLKQLSVKLKNTK"
     gene            4912..5286
                     /locus_tag="PKX09_09350"
     CDS             4912..5286
                     /locus_tag="PKX09_09350"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOU18064.1"
                     /translation="MIYGIGIDLIEVERIARQITGENHFREKIFSPKEIAYCEDKPNQ
                     AEHYAARFAAKEALFKALGTGWRGGLNFAEVEILNDDLGKPAFNFLGKTSQIIEELNL
                     THIHLSLSHLAQIAVAVVILEK"
     gene            5618..>5911
                     /locus_tag="PKX09_09355"
     CDS             5618..>5911
                     /locus_tag="PKX09_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HOU18065.1"
                     /translation="MSNNKLDIKSLESWLWEAACKIRGPVDAPKYKDYILPLIFLKRL
                     SDVFEDELERLAQNYGSKETVEELLAQDHKLVRFYLPKNARWEVIAKHTTQIGE"
BASE COUNT         1655 a         1374 c         1247 g         1535 t
ORIGIN      
        1 atagcggaac aatcgaacat aaaagcgacg gcgctacaac cacctcccta aacgtatcgg
       61 gagcctttct ggcaaatgaa ccgaccgcag cttcggtaat atcacctgac tcggtaaccg
      121 gtaaaacggc tactttatct ttcagcggcg gtgatggagc gcgacgcgtg ctcacaattc
      181 gccccggaac tgagatcgcc tggttaccgg aagatggcaa gccggtcagg ggcgtttcgt
      241 caatttttac tgaggcaatt gatcaaggcg atggcaccag aatagtttat gacggcgagg
      301 atactaccgt tactgttacc ggcttgaccg gcaatacgac ttataccgcg gcgattttcg
      361 aatacaacgt cgcaaccgga aatacttata actacctcac aactcaatat ggcatattta
      421 cctttacaac ggcaatcgta cctgaactta gcgtatcacc gattgtgttg aatttcggcc
      481 gtgtgcttat ttcccataaa ttgataagaa gctatacgct cacgggtaat tatttgaccg
      541 caaataattc gacccgcctg attgccccac ctggttttga tttatcatta gcgcccgata
      601 cgggatttgt gccattattg ccaatcgctt ccacaacact atcctttgat acaaccgtgt
      661 acgttcgttt tacgccaaca gaaccgcata cttattccgg ctttattacc cataccttag
      721 gcaatgatac cgtaactctt gccgtatcag gtcggggagt ctattctctg gtgccgactg
      781 cggagccggt cggattcgcc accttgaacg gcggagcttc cggtgggcaa ggcggcgaca
      841 gcgtcatcgt tactacagct gagcactttt acgaattgat gcacgcgcgc gaaaataaaa
      901 gtacggcacc tctaattgtc cacatttccg gaacgctcag cggctattct tcaaaaattg
      961 ccatcaagcg cactgctaat atctccatag tcggattgga ttctgacgcc gggctgaatg
     1021 gcttcggcat aaaaatcgcc gagtgcaata acatcattgt gcgcaatttg acctttgctg
     1081 attgtcacgt tgatgaaaaa gatgcgctcg agattgacag ttgtcagaac gtatgggttg
     1141 atcactgtac ttttagcgat agtccggata gcgatccgtc cggcagtaat catgacggct
     1201 tactagatat taaaaacggt agccgcaata ttaccgtatc ttataattat tttaccaatc
     1261 atcgccaaac atgcctactg ggacacacgg agagtcagcc tactgacacc gttatgactg
     1321 tgacctatta tcgtaactgg ttcgacggca cttattcgcg tcatccacgg atccgctttg
     1381 ccagagctca tatcgtaaat aatctctaca ccgacatcgg gagctatggg gttggggtaa
     1441 cttgtaacgc tcaagtttta gtcgaggcaa atcactttga aaatacgccc acgccgatat
     1501 taatttctca ggtaaacgac ccgaatagaa ctctcagtgg cgatccagcc ggttatgtga
     1561 aggcgctcga caatttgacg ataaattccg gggcaatcgt cgaaaatctc tccaactacc
     1621 atttcgatcc caacgattat tatagctacg atgtggcacc gataaatatg gtaaaaactc
     1681 tcgttatgga aaatgccggg gctggtcgtc tcgacactaa tcaattagta acacgtaaac
     1741 ctgacattcc atcgcccaat aggtttcagc ttttgccgaa ctaccctaat ccgtttaacg
     1801 cagttaccac catcggcttt cagattcagg ttgctggcaa ggtttccttg aaagcctacg
     1861 atctaatcgg gcgggaggtg gcgacaattt tcgcaggcga gaaaccagct ggatattacc
     1921 ggttcacctt cgacgctaac gatttgccga gcgggattta tctataccaa ctcaagtccg
     1981 gtaatttccg ccagactcgc aaaatgacca ttctaaaata gtatttcgct taatctatat
     2041 gaaaaacaca atgatcaatt atcgaggaaa caatatgaaa aagatgaagc gtaggacatt
     2101 tctcaaatca atgttacttt gcgaattgat tttgctggca tctttcggtt tgctagcaag
     2161 taatctgtgg gcatccggta atgaagttca ccgttacttg agcgaccttc catttgaaat
     2221 gccggaaatg gcgttgccgg aatttcccga tcgggttttc aacgttaccg atttcggcgc
     2281 gattgggaac ggcttgacga tgaataccac agcaattaac aacgctatta aggcttgttc
     2341 gcaatccggt ggcggaacgg taatcatccc aagaggactt tggttaaccg gtccattaga
     2401 gctagctgat aatgtgaacc tgcatttgca gaatggggca ttggtaattt ttagtcccaa
     2461 tcgtagcgac tacccgatta tcaatctgcc gggcaaaggt tttactactc aaccgctgat
     2521 atatggagaa aaattggaaa atatagccat taccggcgac ggcgtctttg acggttgcgg
     2581 tgaaaattgg cgtccggtta aaaaattcaa gatgaccgct tcacaatggc agaacttgtt
     2641 gaaatcgggc ggggtagtca accgctccgg cgacatctgg tggccctcag tagaggcaat
     2701 gcaaggcgaa cattatcgtg attctctcct tgcatctaaa ccgaaaaaac aattaactgc
     2761 ggaagatttc ttgcccgcgc gtgattttct gcgccccgaa ctggtactat taaataattg
     2821 caaaagagtt ctggttgatg gcccaacttt caaaaattca cccaaatggg cattccatac
     2881 ccaacgttgt gaaaacatta ttattcggaa cgtcaaaatt aataacgagt ggtgggctca
     2941 gaacggcgac ggcattgata ttagctcctg caaaaatgtc ttaatctata agtgcaccgt
     3001 cacagccggt gacgacgcta tttgcctgaa atcgagtaaa accaaaggac aaaccgaacc
     3061 cagcctccag aacgtcgttg tgcgcgattg catcgtttat cacggacacg gtggatttgt
     3121 catcggtagt aataccgatg gtggaatgca gaatatcttc gttgacaact gtattttcat
     3181 tggcaccgat atcggactgc gctttaaaag cgcccgcgat cggggcggct tagtagaaaa
     3241 tgtcattatc cgtaatattt atatgaaaga catcgtcaac gaggcaattt tattcaacac
     3301 ttactacgaa aacatcgggc gcgaggaaaa aacctatcca gttaccgaaa aaaccccgat
     3361 ttttaccaat tttcgcctcg acagcatctt ttgttttagc gcccagcaag ccatcggcgt
     3421 ggtgggatta ccggaaatgc ccatcagcca gattgtaatc cgcaattcta ttctatcggc
     3481 agacgtcggc gttacagccg aaaatgcgcg cgatttcatc ctcgaaaatg taaatatcat
     3541 tcctaaaacc gagccgattc tgcacttaac tcaatgcgcc aattttcacc ttgacacaat
     3601 cggattcccc gcaacgattt ctacctttat gaccctatcc ggtaaaaata cgcaagccat
     3661 tcacattaaa aatacggcac tatcttcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnttt ttaagaaatt atacccgtga ctattgatgt gtatttaagc gacttaatta
     3841 attgctaaat aaaaagtgga ttgagggatt tttagacggc tcgccgcaat tcaggcgagc
     3901 cttctttttc aaatggaggt gccaatgcca aaaattttaa ttaccggcgc gctggggcaa
     3961 atcggctcgg aattggttcc agctctctgc cagcgatatg gctcccgcaa tattatcgcg
     4021 tctgacatcc ggccggcaag tgaccaaact attcttagcg ccgcgacctt tgaaacgctt
     4081 gatgttacga attttaatca aattgccgag atcgttaata aatatcaaat aacgaccatt
     4141 tatcatctgg cggctctatt gtcggctgta gccgaagccc atcctcaaaa agcctggcac
     4201 atcaatatga acggacttta caatatgttg gaagttgccc gacaatatca ttgcgccgtt
     4261 tttatgccaa gtagcatcgc cgcattcggc acatcaacac cgcgttatat gactcctcaa
     4321 gacactattc aacgtcccga cacaatctac ggagttacta aagttgccgg ggaattactt
     4381 tgcgattact actttaaacg ttttggcgtg gacacgcgtg gcgtccgtta tcccgggatt
     4441 atctcttcta aaactccacc cggaggggga acgaccgact acgctgtgca tattttttat
     4501 gcggcaatta aacagaaacg ctatacctgt ttcttgaaac cagaaactta tctggatatg
     4561 atgtatatcc cggacgctat caaagccgct attaatttaa tggaagccga cccagccaga
     4621 ttgcaacatc gcaatgcttt caatgttact gctatgagca ttgatccgga aatgctcgcc
     4681 gccgaaatcc gcaaacatat tccggatttt acgatgggct atgccattga tgaggttcga
     4741 cagagaatcg cagactcctg gccaagccat attgatgact ccgccgctcg cgccgagtgg
     4801 gattggcgtc cggactataa cctcgagacg atgaccactg atatgttaaa gcagctttcg
     4861 gttaaattaa agaatactaa ataattcaat ttttcatttt acattccaat tatgatttat
     4921 ggaatcggaa tagacctgat cgaagtcgaa cgcatcgctc ggcagattac tggcgaaaat
     4981 cacttccgcg agaaaatttt ctcccccaaa gaaatcgctt attgcgagga caagcccaat
     5041 caggccgaac actatgccgc ccgttttgcc gccaaggaag ctcttttcaa agctctcggt
     5101 accggctggc gcggcgggct gaacttcgct gaagtagaaa tacttaacga cgatctcggc
     5161 aaaccagcat tcaactttct gggaaaaacc agccaaatca ttgaagaatt aaacctgacc
     5221 cacatccatc tttcgctgag ccatctggca caaatcgccg tagcggtcgt cattctcgaa
     5281 aaatagcatc ggtttataaa aagtttcctt cttcggtaac cgtattaatt tttgagaaaa
     5341 ttattaatgt tcaggtcatt acgcctagca gaaaaaattc atcgagatgg cgattcttaa
     5401 taggtaaaaa tttaaataat gggccaacaa tctataaaaa gttgcttaac tggttttatt
     5461 tcagttagcc gattaaattt tgtcattaaa aaagtgaaac cgttaatcga aattatgtct
     5521 gctatgaact ctcatcgagc aactccgttc tatttgctaa aaatagaaaa caaaatgaaa
     5581 gccttagaag atttctattt aatacaagga ctttactatg tcaaacaata aactcgatat
     5641 caaatctctg gaaagctggc tctgggaagc cgcgtgtaag attcgcggac cagttgacgc
     5701 accaaagtat aaagattaca ttttaccgct aatcttcttg aagagactct cggatgtatt
     5761 cgaagatgag ctcgagcgac ttgcgcaaaa ttatggctcg aaagaaacag tcgaagagtt
     5821 gctcgctcag gatcacaaac tcgtgcgttt ttatttgccc aaaaacgctc gatgggaagt
     5881 gattgcaaaa catacaacgc aaattggcga a
//
LOCUS       DAOSMP010000081         5882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_13705_length_5882_cov_21.602025, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000081 DAOSMP010000000
VERSION     DAOSMP010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13705_length_5882_cov_21.602025"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /gene="cysS"
                     /locus_tag="PKX09_09360"
     CDS             complement(<1..962)
                     /gene="cysS"
                     /locus_tag="PKX09_09360"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOU18066.1"
                     /translation="MSIYFYNTLSRQKEEFRPIEEGVVRLYTCGPTVYNFAHIGNYRA
                     FVFEDLLRRFLKFKGFRVIQVMNITDVDDKIITAANREGVSIREFTEPFRQAFMEDLT
                     TLGIEPAEYYPRATEHINEMVALIETLLKKGVAYRSEDGSIYFKVSEFPRYGQLANLN
                     VSEMRRGNRVANDSYEKEEIRDFALWKAWKPEDGAVFWETSLGKGRPGWHIECSAMSM
                     KYLGRHFDIHTGGVDNIFPHHENEIAQSEAATGEKFVNYWIHCEFLLVNDEKMSKSIG
                     NIIYLRDLVKKGYNPTAIRLTLLGTHYRQKLNFSFEKLEESGKI"
     gene            complement(964..1560)
                     /locus_tag="PKX09_09365"
     CDS             complement(964..1560)
                     /locus_tag="PKX09_09365"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HOU18067.1"
                     /translation="MSDKKNVDPELIKSNSNSLDTKGKHWYAVYTRPRHEKKVNGLLQ
                     EQQIESYLPLVSTKRQWGNRWAQVEEPLIRSYVFVHIHLNQTLYVLETYGVVRFVMFA
                     RELAVVPDFQIEALKKSLESGFVLSPINYMQVGQLVEVTEGPLKGVIGKIQQIGKNER
                     FVLTLDAVQAAYTIQIDPQWLRPLSPEKKKIFTLPLGF"
     gene            complement(1557..2546)
                     /locus_tag="PKX09_09370"
     CDS             complement(1557..2546)
                     /locus_tag="PKX09_09370"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOU18068.1"
                     /translation="MKIAVLMGGVSSEREVSLSSGRNIVAALRENGHEVLALDTVLPI
                     EQLSQDIQPTGEHLQKGEENLICLLSEATVRAVDFVFNALHGGSGENGAVQSLLEIMG
                     YKYNGSDHEGCAIAMDKIVSKMIFEGIGIRTPEWISFDLKVGYDPVAIQEEILRKIEP
                     PLVIKPAHEGSTVGLSIVKEVAGIQPAIATAFKYNRALLVERYIPGREITIAILGEMA
                     LPLVEIRPKHGIYDYECKYTSGMSEYIVPALVDEKIVKQIQEQALVAFKALRCSGYGR
                     FDLRLTDEGNAFFLEFNTLPGMTATSLVPKAAKAIGMNFNALLEKIIEIGRDR"
     gene            complement(2546..3106)
                     /locus_tag="PKX09_09375"
     CDS             complement(2546..3106)
                     /locus_tag="PKX09_09375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOU18069.1"
                     /translation="MNRAVFLDRDGTLNYDSRDYIKNVAEFQLFPNTVAALKKLYSAG
                     FKLVIITNQACVAKGLTTVAAVEEIHQLLREKLSEAGIELAGIYYCPHDPEAGCDCRK
                     PRIGNILLAARELEISISDSYFVGDSHRDIEAGRAAGCRTILVTTGVRQASYADIAEW
                     QVQPDFVVPDLLTAAEMILSLERKMN"
     gene            3484..4254
                     /locus_tag="PKX09_09380"
     CDS             3484..4254
                     /locus_tag="PKX09_09380"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOU18070.1"
                     /translation="MTKKPFYISIICCLLIKTGLNGVELALKLPPPNPIAQPLQYLQY
                     YPPHYWNGLQKTLDWKSNAPLFVAGAILIPMSFLGDQPLQENVAKHNFLPENISRIGY
                     IYGHRWGNYAAAVAVGINGVIQHQPWNKTFSQIELLATSTITTALVTEAIKLATHRER
                     PNEENYKSFPSGHSSNSFALAAGLDGIYGYKIGIPAYLMAIFVASTRINDNKHYLSDV
                     VAGAFIGTWIGKSFSRQYQLEWQISATGSPAISITIPF"
     gene            4266..5159
                     /locus_tag="PKX09_09385"
     CDS             4266..5159
                     /locus_tag="PKX09_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOU18071.1"
                     /translation="MSFLSAVQQARFLILDGALGTELARRGIVIELPLWSANAILHNP
                     QAIVEIHSDYIRAGADIITTATFRTDSLTFARAGLNKKQAAEATFRAVELAREAIADV
                     HSTRQIWIAGSIAPLADCYEPESAPDFNMALRIHRQRAQWLAEAGVDFALLETMNNLN
                     EARAASIAALETGLPVFTSFILAENGVLLSGEDIWLVMSEIKKLGVAGLGINCTHHSL
                     ISNFLENYGRKLDLPLILYANAGIYSRKNGWCEDPNFTPQNYARIAANWLKSGAKIIG
                     GCCGTTPDYIKALVDLRKSPV"
BASE COUNT         1651 a         1372 c         1190 g         1669 t
ORIGIN      
        1 atgatcttgc cggactcttc gagtttctcg aaactgaagt ttaatttctg ccgatagtga
       61 gtacctaata aggtcagccg aatagcagtc ggattatatc cttttttgac caaatcccgc
      121 aggtaaataa tattgccgat tgactttgac attttttcat catttactaa taaaaattcg
      181 caatgtatcc aatagttaac aaatttctcg ccggtggcgg cttcgctctg ggcaatttca
      241 ttttcatgat gaggaaaaat attatcaacg ccgccggtat gaatatcgaa atgccgtcca
      301 agatatttca tactcatcgc ggaacattcg atatgccagc cgggtcgtcc cttaccgagc
      361 gacgtttccc agaaaactgc accgtcttcc ggtttccagg ctttccagag tgcaaaatca
      421 cgaatttctt ctttctcgta ggaatcattg gctactcgat tgccacgccg catttccgaa
      481 acattcagat tggctaactg cccataacgg ggaaattcgg aaactttgaa ataaatcgaa
      541 ccatcctcag agcgataagc gacacccttt ttcaacaagg tttcaatcaa tgccaccatc
      601 tcattgatat gttcggtagc gcgcggataa tattcagctg gttcgattcc caacgtcgtt
      661 aggtcttcca taaacgcctg ccgaaagggc tcggtaaatt cgcgaatgga aacgccctcg
      721 cgattcgccg ctgtgataat tttatcatca acatcggtaa tattcattac ttgaataaca
      781 cggaaacctt tgaatttcag gaatcgtcgc aataaatctt cgaaaacgaa agcccggtaa
      841 ttaccgatat gagcaaaatt atagaccgtt ggcccacagg tataaaggcg aaccacacct
      901 tcctctatcg gacggaattc ttccttttgg cgactcaggg tattataaaa ataaatgctc
      961 atattaaaat ccgagtggta gcgtgaaaat tttcttcttc tcaggactta acggacgaag
     1021 ccactgggga tcaatctgaa ttgtataagc ggcttgcacc gcgtcgagcg tcagaacaaa
     1081 tcgctcattt ttgccgattt gctgaatctt tccgattacg ccttttaatg gtccctcggt
     1141 aacttcaacc aattgtccaa cctgcatata attaatcggc gacagaacaa aaccgctttc
     1201 caggctcttt ttcaaagctt caatctgaaa atcgggcacg accgccagct ctcttgcaaa
     1261 cattacaaag cgcaccacac cgtaggtttc cagaacataa agcgtctgat tgagatgaat
     1321 gtggacaaag acgtagctac gaatcaatgg ctcttctacc tgagcccagc ggttgcccca
     1381 ttgtcgcttc gtggacacta aaggtaaata gctctcaatc tgttgctctt gaagaagtcc
     1441 attgactttt ttttcatgtc gcggtcgggt atagactgca taccaatgtt tacctttggt
     1501 atccaaacta ttggaattgg atttaatcaa ctccgggtca acattttttt tatcgctcat
     1561 ctatccctac cgatttctat aattttttca agtagagcat tgaaattcat accgatagct
     1621 ttggcggctt tcggtactaa actggtcgcc gtcataccgg gcagggtatt aaattccagg
     1681 aaaaaggcat tgccttcatc agtcagacgc aaatcaaaac ggccgtatcc agaacaacgt
     1741 aaagctttga aagccactaa cgcctgttct tgaatctgct taacaatttt ttcgtccacc
     1801 aaagccggca caatatactc actcataccg gaagtgtatt tacattcata atcataaatg
     1861 ccatgcttgg gacgaatttc aaccagcggc aaagccattt caccgagaat cgctatggta
     1921 atttcgcgcc caggaatata gcgttcgacc aacaaagccc ggttgtattt aaaggcggtg
     1981 gcgatagccg gttgaatccc ggcaacctct ttcacaatgg agaggccgac ggttgagcct
     2041 tcatgggcgg gcttgatgac cagcggcggt tctatttttc gtaatatttc ttcttgaatc
     2101 gcgaccggat cgtacccgac ttttagatcg aaagaaatcc attccggcgt tctaatccct
     2161 atgccctcga agatcatctt cgaaacaatt ttatccatag caatcgcaca accttcatgg
     2221 tcactgccgt tatatttgta ccccataatt tcgagtaggc tttgaactgc cccgttttcg
     2281 ccactgccgc catgcagggc attaaaaaca aaatccacgg cgcgaacggt cgcctcgctt
     2341 agcaagcaaa tgaggttttc ttcgcctttt tgcaaatgct cgccggttgg ttgaatgtcc
     2401 tgacttaatt gctcaatcgg taaaactgta tcgagcgcca gcacctcatg cccattttcc
     2461 cgtaaagccg ccacgatatt gcggccactg gagagcgata cttctcgctc ggacgaaact
     2521 ccacccatca ggacggcgat tttcattaat tcattttcct ttctaaactt aaaatcatct
     2581 cagccgcagt cagtaagtct ggaaccacaa aatccggttg gacttgccat tccgcaatat
     2641 cggcataact ggcttgacga acaccagttg tgactaaaat agttcgacaa ccggcggcgc
     2701 gtcccgcctc gatatctcga tgtgaatccc ccacaaagta cgaatctgat atactaatct
     2761 caagttcacg ggcggcaaga agaatattac cgatacgcgg tttgcgacaa tcacaaccgg
     2821 cttccggatc atgtggacaa taataaatac cagccaactc aatgccggct tcgcttaatt
     2881 tttcccgcaa tagttggtga atttcctcga cagcggcaac cgtcgtcaaa cctttagcaa
     2941 cgcaagcctg gttggttatt atgactaatt tgaatccagc tgaatataat ttctttaaag
     3001 ccgcgaccgt atttggaaaa agctgaaatt cagctacatt cttgatataa tcgcggctat
     3061 cgtaattcag ggtgccgtcc cgatctaaaa acactgctct attcacttta ataaaattac
     3121 ctgtcctaaa tttagcgccg atgctgtgga taagtttgtc aacttaaata acataaaata
     3181 aactattatc tgactttggc atacttggct gtcctcgcct tctacatcct caaacatcgc
     3241 tattgacact caccttcatt tctccataaa aatccagaca taaattacaa tccggaggcg
     3301 taaaataata gggcagggca taaaaaaacc tgcagaaggt tttatttatg atttctttct
     3361 gttcgcggtt ctccgaacca gccttcagaa gattggcctc tattattttt tgatatgttt
     3421 tgagttgtat taaaacttaa attacttccg aattgtctgt actttagaaa aaatatgaaa
     3481 aaaatgacca aaaaaccatt ttatataagt atcatctgtt gccttttaat taaaaccgga
     3541 ttgaatggcg tcgaacttgc gctgaaattg ccaccgccta atccgattgc gcaacccctg
     3601 caatacttac aatattatcc acctcattac tggaatggtt tgcagaaaac cctcgattgg
     3661 aaatccaatg cgccgctttt tgtagcgggc gccatcttaa taccgatgtc ctttttaggc
     3721 gatcagccac tgcaggaaaa tgtcgccaaa cataattttt tacctgagaa tatttcacgg
     3781 atcggctata tttatggaca tcgttggggc aattacgccg ccgctgtggc tgtgggaatc
     3841 aacggcgtca tccagcatca gccctggaat aaaacctttt cccaaatcga attgttagcc
     3901 accagcacca taaccactgc tctggttaca gaagcgatta aattagcaac gcatcgggaa
     3961 cgtcctaatg aagaaaatta taaatcattt ccctcaggac attcctccaa ttcctttgca
     4021 ttggcggctg gtctggacgg catttacggt tataagattg gtatcccggc ttatctaatg
     4081 gcaattttcg tagcgtccac ccgcattaac gataataaac attatctttc cgatgtagtt
     4141 gccggtgcgt ttatcggcac ctggatcgga aaaagtttta gtcggcagta tcagctcgag
     4201 tggcaaattt cagctacagg atcaccggca atttctatta ctataccatt ctgaagggga
     4261 attttatgtc ttttctatct gctgttcaac aagcgagatt tttaatactg gatggagctc
     4321 tcggtacgga attggcacga cgcggtatcg tcattgaatt acctctctgg tcagccaatg
     4381 ccattctaca taatcctcaa gcgattgttg aaattcattc agattatatc cgagccggag
     4441 cggatattat cacgactgcc actttccgca ccgattcgct gacctttgct cgagccggac
     4501 tgaataaaaa acaagccgcg gaagccacat ttcgggcggt tgaactggct cgcgaagcca
     4561 ttgctgatgt tcactcgaca cgtcaaatct ggatcgccgg ctcgatagcg cctctggctg
     4621 attgctatga gccggaatcc gcacccgatt tcaatatggc acttcgaatc catcgccaga
     4681 gagcccaatg gttggcggaa gccggggttg atttcgctct cctggaaaca atgaataatc
     4741 tcaacgaagc cagagcggct tctatcgccg cccttgaaac cggactgccg gtttttacca
     4801 gcttcatcct cgcggaaaat ggtgttttat tgagcggaga agatatttgg ttagtgatga
     4861 gtgaaatcaa aaaactcgga gtcgccggac tgggaattaa ttgcactcac catagtctaa
     4921 tctcgaattt tcttgaaaat tacggacgga aacttgactt gccgttaatt ttatacgcca
     4981 atgctggaat ctattctcgt aaaaatggtt ggtgcgaaga tcccaatttt acaccgcaaa
     5041 attacgcccg gatcgcggca aattggctaa aatcaggtgc taaaatcatt ggcggttgct
     5101 gtggaacaac tcctgactat atcaaggctt tggttgattt aagaaaaagt ccagtgtgat
     5161 ggggaatcac gctggactgg agggtagtga ggtttggagt tttcaaaaaa ttctaactta
     5221 atttaaccca aaagccgagc tggatttatg ccacgcgtca caattttccc gggcattaat
     5281 actattttca agaccttcgc caatttttta gaatgtttaa cttctacgaa gtaaggattg
     5341 ctgataattg tatttttcta taaaacttcc actacctcag ccaatttctg ccacgccgca
     5401 ggcggggtta aattggtttc gacgcaatat ttagagcgag tggttaatag cttagtactc
     5461 accgcgtagc ggtcaaagta ttgtagaata taaaaatcag agaagttatt cctcggagag
     5521 gatcaagaag aaactatttg ccgcaggaaa tctttatttg actaagatta gttccagcgt
     5581 tatgcgcttg gctaatattt caacaagatt tcatcgtaat gttgtttgcc aggttatcag
     5641 aacttcttcc taattttata gaaagttaaa cctctccgag gtagcaattt ctggcaatta
     5701 tatttttcta caaaacttga agccctacgg gctagttgga aatctatggt tttcagataa
     5761 acaattaccg cggagcggtc aaggtattgc agaatagaaa agtcagagaa attattcctc
     5821 ggagaggata gaggagaaac tatcatttcc gaatgatttt catagattat caaatgacaa
     5881 aa
//
LOCUS       DAOSMP010000082         5606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_14598_length_5606_cov_5.665826, whole genome shotgun sequence.
ACCESSION   DAOSMP010000082 DAOSMP010000000
VERSION     DAOSMP010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14598_length_5606_cov_5.665826"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(308..790)
                     /locus_tag="PKX09_09390"
     CDS             complement(308..790)
                     /locus_tag="PKX09_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18072.1"
                     /translation="MKKQLLPIIIVCLSLTGCANNGKIETVVTPTANELIEFEVPEPI
                     PQPISGKGSVTGQILTDNSQELVGLIVYLGELIQLPENTYGAYLDTKSAPSSLISIEN
                     GKFYFKDVEPGIYSLIIYEVMMGGKVYQDTNGSAIPIEVNEGEITNVGKIKFFFRGNE
                     "
     gene            complement(886..2367)
                     /locus_tag="PKX09_09395"
     CDS             complement(886..2367)
                     /locus_tag="PKX09_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18073.1"
                     /translation="MKKFFVYAMIGLIFIIGLNPTIAYALPAGDWASGIKIQNLDPAN
                     AANLAVDLYDEEGTLEISITTTANGNPLIAPIGGSIELYMPAYVSVPSGQYSAVVSSS
                     TAVGAVVTSTNYESGMADSYASMEPSTAISIPYVYHNHNHYHTEITLLNTTNNEATVV
                     VNFKEPSSSVAFGDSGSHEKSINITIAGNGSITIDTSTAQFNDLGWFIGAASLTSTQP
                     LTAVANQYRVVGSGDTKGNLMISSRGLSSADSGTQLVVPSLYKQFIGASGTWQSGITI
                     QNPNDSTASVQVVFTADPDMPAWTGTKSLTINANDSVELYLPGVILDSEDPIPDMFKG
                     SAIITSDVSIVANVQHTNYSGANGYGVGMGYTAFSQGGKKLSLPSLYNWPSGAGVWIS
                     GIKVQNLTSTSVVFKATFVPDPDSVSQFTGTASNIALSGYGATELYFGAAFLDGGEAI
                     PSGWKGSAIIEVTTGDSDIAATVIHTNYGRHVANMYTAIVIPE"
     gene            complement(2483..3091)
                     /locus_tag="PKX09_09400"
     CDS             complement(2483..3091)
                     /locus_tag="PKX09_09400"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU18074.1"
                     /translation="MKSKNSVITGNYFNKYKSKNLLYSILVDHYRFALQSLIQDLPID
                     TCLEIGSGEGYIIDYILSTKPGIQIFGSDIGFSVVSLAKTDRQFVKWCVSDGKNIPIT
                     ENSFDIVFACEVLEHIDKPIDVLKEINRVSKKYVIISVPFEPFWRIMNIMRLKYLSSL
                     GNTPGHINHWSINGITKLVEVYFQPIDVRISFPWIFIKAQKR"
     gene            complement(3233..5137)
                     /locus_tag="PKX09_09405"
     CDS             complement(3233..5137)
                     /locus_tag="PKX09_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18075.1"
                     /translation="MDNPITIVDISFISWLRLLFGLILFLGPGKLLLSISPFRKEFDR
                     SIAFIVTFGYSVAIWAILLSISYLFKINIPTHIAIFFYLLCWLIYIIIDKPHKSYSLN
                     LNKVSFYRISLWIFIFLTFMIRIWIVRFEVAGLGSDSYHHTLFAQMIRDNGMIPMNMG
                     SNYPVITFTYHFGFHAIVAFLGWISGLPTRLLVLILGYILLAICSLAVGFSTERITGS
                     KIAGFAATICVACFFIFPSYMLMWGRYTQLTGLTLISIFLIPFWSWINNDYSKSGIIE
                     LGILAAGTGLTHYRMVAFLVIGVLVIVVFQLLGKETSNWKKGIKGGILLFFTSLSFLV
                     PWFIHLWQSFQVGYPVEASLPASAFFSIDRLGQEALNYPLNLAALILVGISILLGILH
                     KDKIIIAITLWCLIALLPSRNIQLLDTVSVLISLGIPLAILVGVAISKFCDFITSSKF
                     SPFIKNIITPVLVGALGISGIIGTLTYPTTLDSYLKASDLKAFEFINEDIPMDAKFMI
                     NIYRFNFSDILMVGSDGGYWIPLLTGRETVVPPMVFTNERVSDPIFVENLRMLGKLNG
                     DLTSPESLDMLSYQGITHVYIGERGGPINPVDLLSSPYFKLVYESNSVYIFEYNQKGA
                     FPRSLLRKIE"
BASE COUNT         1870 a         1080 c          947 g         1709 t
ORIGIN      
        1 gcggcgtaag catttggctg attgagtaaa aaatgagttt aggaacagag tggaggactg
       61 ggtagagtta atgagcatct gtagaatgca ctcagcatta attcagggta tcatagaatt
      121 gaattgagag aaaacgaata cttaatcact aggagtttat gatcgtctgc tatttcggca
      181 tatcttgtgg aattattttt aatattacat gttaattgta aaaaccacca tttcgaataa
      241 aagtaatctc caagtattaa aaatagggta aacagtaatt attataagat taataataat
      301 aatataatta ctcgttccct ctaaagaaaa atttaatctt gcccacgtta gtaatttcac
      361 cttcattgac ttcaattggt attgcgcttc catttgtatc ctggtaaact tttcccccca
      421 tcattacttc ataaatgatt aatgaataaa ttcctggctc gacatcctta aaatagaatt
      481 tgccattttc tattgaaatt agtgaagatg gtgcagattt ggtatcaaga tacgctccat
      541 atgtattttc gggaagttga attaactcgc ccagataaac aattaatcct accaattctt
      601 gtgaattatc tgtaagaatt tgtccagtaa cagaaccttt tcctgatatt ggttgaggta
      661 ttggctcggg tacttcaaat tctattaatt cattggcagt gggagtaact actgtctcta
      721 tcttaccatt attggcacaa cctgttaaac taaggcaaac aataattatt ggtaataatt
      781 gttttttcat agaatgatta tatcttgaat agagttagaa tattaattaa gtaacacgcc
      841 gggaaacata ctcgacgtgt tacttattct gagttatcag ttttattatt cgggaatcac
      901 gattgctgta tacatatttg caacatggcg tccataatta gtatggataa ctgttgcagc
      961 aatatctgaa tctccggttg taacttcgat tatagccgaa cccttccagc ctgagggaat
     1021 ggcttcacct ccatctagga atgcagcacc aaaatataac tcggttgcac cataacctga
     1081 aagggcaatg tttgaagcag ttccagtaaa ttggctcaca gaatctgggt ccggaacgaa
     1141 agtagctttg aatacaacag aagtagatgt taaattctgt accttgatcc cagaaatcca
     1201 tacccctgcc cctgagggcc agttatataa ggatggcaag cttaatttct ttccgccctg
     1261 gctaaatgcg gtgtaaccca ttccaacgcc ataaccattt gcacccgagt aatttgtatg
     1321 ttgaacatta gccactatag agacatctga tgtaattatc gctgacccct taaacatatc
     1381 aggaatagga tcttcggaat caaggattac acctggtaag tataattcaa cactatcatt
     1441 agcattaata gttaagcttt ttgttccagt ccatgctggc atatctggat ctgcagtaaa
     1501 aactacctgc acactcgccg tcgagtcgtt aggattttgt atcgtaatac cagattgcca
     1561 agttccacta gctccgatga attgcttata taatgatgga actactaatt gagtgccact
     1621 atctgcacta gacaaacccc ttgagctaat cattaggtta cccttcgtat caccagaacc
     1681 aacaacccta tactgattag ctactgcagt taagggttga gttgaggtaa gcgatgctgc
     1741 gccaataaac caacctaagt cattgaactg agcagtcgaa gtatctattg tgatggaccc
     1801 attaccggcg atagtaatgt taattgattt ttcatgagaa cccgaatcac caaaggctac
     1861 tgaagagctc ggttctttga aatttactac aacggtcgcc tcattatttg ttgtgttcag
     1921 aagagtgatt tcggtgtggt agtgattatg attatgataa acatagggaa tagaaatagc
     1981 tgttgatggt tccatcgacg cataactatc agccataccg gattcatagt ttgtgcttgt
     2041 aaccacagcg ccaacggcag tagaagatga aacgacggct gagtattggc cacttggaac
     2101 acttacgtac gcaggcatgt acaactcaat tgatccacca attggtgcaa ttaatgggtt
     2161 gccatttgca gtcgttgtta tggaaatctc taatgttccc tcttcatcat ataaatctac
     2221 cgcaagatta gccgcattgg cgggatctaa attttggatc ttaatcccag atgcccaatc
     2281 ccctgctggt aaggcatagg caatcgtagg attaagacca attataaata ttaatccaat
     2341 cattgcataa acaaaaaatt ttttcatttt atcctccgtt caatttttta taagtatact
     2401 tgtctcatgc caaaaaacaa taatacttat agattatttt tcgactaaga agaattttac
     2461 aggtatttcc attcttaatc ggttatcttt tttgagcttt aataaaaatc cacggaaaac
     2521 taatccgtac gtcaataggc tgaaaataaa cttctacaag cttagttatc ccatttattg
     2581 accaatgatt aatatgaccg ggagtattgc ctagtgaact taaatacttt aatctcatta
     2641 tattcataat acgccaaaat ggttcaaatg gtacgcttat aatgacatat tttttgctca
     2701 ctctattaat ttcttttagg acatcaattg gtttatcaat atgctctaat acttcacatg
     2761 cgaacactat atcaaatgaa ttttccgtga ttggtatatt cttcccatcc gatacgcacc
     2821 attttacaaa ttgtctatcg gttttagcta aagaaaccac cgaaaagcca atatcgctac
     2881 caaatatttg aattccgggt tttgttgaca aaatataatc aataatatat ccttcgccac
     2941 ttccaatttc taagcaggta tctattggta ggtcctggat tagagattga agggcaaatc
     3001 tataatgatc aactaaaata ctatacaaca aatttttcga tttgtattta ttaaagtagt
     3061 ttccagtaat aacactattt ttacttttca atttaattct ccgaaattag gcaagtaaaa
     3121 attagctttt ctatctgatg tcactataag tgaccggcgt tgagtaagat ttaataataa
     3181 taataatata atttaagttg ttcgtcatca gcatatcact tatttatcta tcttactcta
     3241 tttttcgcag caagctgcgg ggaaatgccc ccttttgatt atattcgaaa atgtatacag
     3301 agttactttc atataccaat ttaaaatacg gtgaacttaa taaatccact ggattgattg
     3361 gcccaccccg ttcccctata taaacatggg ttatcccttg atagctcaac atatccaggc
     3421 tttctggtga ggtcaagtca ccatttaact ttccaagcat tctcaaattt tcgacaaata
     3481 taggatctga gaccctctca ttggtaaaaa ccattggagg aacaaccgtt tctcttcctg
     3541 taagcaatgg aatccagtaa ccaccatcac ttccaaccat cagaatatcg gaaaaattaa
     3601 aacgataaat attaatcata aactttgcgt ccattggtat gtcttcattt ataaattcga
     3661 atgctttgag gtctgacgct tttaaataac tatccaatgt tgtagggtaa gttaatgtgc
     3721 caataattcc gctaattccc aatgctccca ctagaactgg tgtgataata tttttgataa
     3781 atggagagaa tttacttgaa gtaataaaat cacaaaactt acttattgca acaccaacaa
     3841 gaattgctag tggaattcct agcgatatca aaacactaac tgtatctaga agttggatat
     3901 ttcttgaggg taataaggcg attaagcacc atagggtaat cgcgatgata attttatctt
     3961 tgtggagaat tcctaagagg atagaaattc caaccaagat taaagcagct aaatttaatg
     4021 gataatttaa agcttcctga ccaagcctgt ctattgaaaa aaaagcagat gccggaagag
     4081 atgcttctac aggataacca acttgaaacg attgccacag atgaatgaac catggcacca
     4141 aaaacgataa ggaggtaaaa aataataaaa taccaccttt aatacctttc ttccaattgg
     4201 atgtttcctt gcctaataat tggaaaacaa caataacaag cacgccaatg actaaaaatg
     4261 ccaccatccg ataatgggtt aatcctgtcc cggcggcaag aattcctagt tcaatgattc
     4321 ctgatttact ataatcatta tttatccagg accaaaatgg aattaaaaat attgaaatta
     4381 atgtcaaacc agttaattga gtatatcgcc cccacattag catataggaa ggaaaaataa
     4441 agaagcaggc aacgcagatt gtagctgcaa aaccggcaat tttagaacca gttattcttt
     4501 cagttgaaaa cccaacagcc agactacaga tcgctaataa aatgtaacct aaaattaaaa
     4561 ccaataatct ggtcggaagc ccactaatcc atcctaaaaa agctacaatt gcatgaaaac
     4621 cgaagtgata tgtgaaggtg attactggat agtttgaccc catattcatc ggaatcattc
     4681 cattatctct aatcatctgc gcaaataaag tatgatggta gctatcactt cccagacccg
     4741 caacctcaaa acgaacaatc cagattcgaa tcatgaaagt gaggaaaatg aagatccaaa
     4801 gactaattcg atagaatgaa accttattta aatttagaga ataagactta tgtggtttat
     4861 ctataattat ataaataagc caacataaca aataaaagaa aatagcgata tgagtaggaa
     4921 tattaatttt aaataaatag cttatactta ataaaattgc ccatatcgct acagagtatc
     4981 caaaggtaac tataaatgct attgagcgat cgaactcttt ccggaatggt gatatcgata
     5041 gcaacagttt tccaggaccc aaaaatagga ttaaaccgaa tagaagtctt aaccaagaaa
     5101 taaaagatat atctacgatt gtaatcgggt tatccataat tccaatcttt tctttcccct
     5161 ttacccacaa aatttggaag cctatgttta gttgggctat cgtctaaaac agcgaataaa
     5221 tatgcccaca gccctgaccc tggacgcttt attacaaaaa gcgccacact ggggtgaatt
     5281 aatacagtaa agtaattccg ccaggaagga ataaaaagcc gaattcggct attgctttct
     5341 cgttcttgat ctgctaattt tttgtacctc ttattctgca cacaaataat tttatctcat
     5401 catccgttgc actagatcac tgaaaccaaa tttaccctca aataattcca gagcataatc
     5461 ggtggaagaa gtgttgagct ggtttataat cgacaccacc acactaatcg cgaaactact
     5521 caatttttac ttccattaaa acaacacatt agaaagatcg gcgcggtatt ggcgcacgtc
     5581 cggatgatgc tcacctagga cctcga
//
LOCUS       DAOSMP010000083         5559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_14752_length_5559_cov_16.344840, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000083 DAOSMP010000000
VERSION     DAOSMP010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14752_length_5559_cov_16.344840"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PKX09_09410"
     CDS             complement(<1..316)
                     /locus_tag="PKX09_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOU18076.1"
                     /translation="MDRIVQHPILTIPPARKLVKFTFNDTILEGFEGEPVSSALMAQG
                     YREFSIHRKGDTPQGLFCANGQCSHCTLVIDGLAQKSCVTPLKGGMQIKTLRHIPEIP
                     ADN"
     gene            923..>1300
                     /locus_tag="PKX09_09415"
     CDS             923..>1300
                     /locus_tag="PKX09_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOU18077.1"
                     /translation="MIKNIHRIIGLLCLIIWIDCEGSDYRALIETGEFKKAEEMIRRE
                     LFSNQSLSVSERRTLEFELERMRRIRLDFNKSQAEVIEYIKQYVPDVTPTDLVRWESE
                     KSLEYLVIDGEKMYFKDAAPNLFR"
     assembly_gap    1301..1400
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1401..2165
                     /locus_tag="PKX09_09420"
     CDS             <1401..2165
                     /locus_tag="PKX09_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOU18078.1"
                     /translation="NTNQTLQRTIYFEKAAVKDSTTHFSVEYTYEIYGSYTPVRAEKV
                     IPVEPNPDLNPFLSEEPPHIVFTDEIRQLSQRIVGSETNPYRIAQKLFEWIDVNIVWA
                     SAREYSTIYNIPQYVINNRHGDCGMMVLTFITLCRLNGIPARWQSGWEFKPPQDSMHD
                     WGEIYLAPYGWVPMDVTYGRRATADESFRWFYLSGMDSYRLIFNDGMAADFYPLKIYP
                     RSETIDSQRGEVEWEGGNLYFNQWNWEHDWEIISEP"
     gene            2187..4346
                     /locus_tag="PKX09_09425"
     CDS             2187..4346
                     /locus_tag="PKX09_09425"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU18079.1"
                     /translation="MKINTKKILIGLGIALIITLLSGLLYHNGLIQRWENQVNDILYR
                     DFQVEQHPSEAVIIAIDQNSLDYFQKNFQILWPWPRDIYAMATNYLNSCGAKVIVYDI
                     IFSTPDIDRLNVQAEYADSAFARAMLSSGKVILAMQMEDSTHLSVEKLPTQFNRQIVY
                     NVPQRIVYKYPNVTAPIERFQKAMAYPGAVNFFTDNDGVCRRVPLFFAKDHQIVPYMA
                     LAAVMIYDDISSIDYDSMRHTIFAGKRRVPLNNSGFFDIYWYGPGGAGNTFQYVSFAQ
                     LLNSYLQVQNGQKPDVDPAIFQDKAVFIGATAAGLLDLKTTPFSSVEPYPGVEIYATI
                     FSNIIRGDYISNLPFIIWLGFSLILIWLLAFLWQKLKIWQSAGISLIILFIPLIMSVM
                     IFQRLKMFMPVVTTEIAFVLSITVVLLVNYLTEGREKKMIKKVFNRYLHPTVVEVLTK
                     HPERFEMGGKEIVATVMFSDLQNFTGIAELFTPPEIVGFLNQYFTKVEQIIFKNDGML
                     DKYTGDGIMAIFGAPIPTNEHARMACNAALDFKNLSTFTIEVKNRTIPLITRLGINSG
                     NFVVGNIGSANRMDYTAIGDTVNLAARLEGVNKIYGTQNIISETTYSLVKENFICREL
                     DLIRVKGREKPLAIYNIICIKDDLSSEIEKMLKLYNEALRLYRECEFQTAERAFLELS
                     RQFPDDSVGKVFAKRCVQLINEPQLIDEDGVFNITVK"
     gene            4415..>5559
                     /locus_tag="PKX09_09430"
     CDS             4415..>5559
                     /locus_tag="PKX09_09430"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOU18080.1"
                     /translation="MKRFILIILVVSVLIVTVFAEESVVIKRENTHFRQGPGSYYPLI
                     GMLQKGVQVAAVGTQSGWLKVQWAGQTGWISENAVIASDEPTGSILTGSISGNGSMMI
                     SRASASGAVKGFAQKFVNFHDGDPAFIEQYDVELFTPAEFQRFRQETFRGRDPEKIRK
                     RYKRIKSENTDHSISLQDEKIGLAAASRIAASGLVANQSQLKYINLVGNIVLENTDMY
                     DYPFKFYILKDSHPSAYAIPNGMIFVTSGLLELLQDEAELAVILAHEISHVVQKHGAE
                     EIAKRKTMIVADEGFNELDQEIVTEDATADELDLIALNCYETATKRRQIEYEIEADQL
                     GMIYAYRSGYDPSALWRVLTRIEANTSPDFWNPESNWSYDAVFDRAER"
BASE COUNT         1510 a         1106 c         1246 g         1597 t
ORIGIN      
        1 ggttatcggc tggaatttcc ggaatatggc gcagggtttt aatctgcata ccgcctttta
       61 acggcgtcac acaggatttt tgcgccaaac cgtcaatgac taaagtgcaa tgcgaacatt
      121 gaccattggc acagaagagt ccctgcggcg tgtcaccttt gcggtgaatg gaaaattccc
      181 gatagccctg cgccatcagc gccgaagaaa ccggttcgcc ttcaaagcct tccagaatag
      241 tatcattgaa agtaaatttt actagttttc gagcgggtgg aatcgtcaaa atgggatgct
      301 gaactattct gtccattttc gcctttgtat ttaacaggat ttgtaatcat aaaataattg
      361 gtcaaattta gatatacggt ggcttaatga aaatatttaa tgaggaaata ttattaaaaa
      421 atttacgaat aaatttacgg gctattctaa acctgtttcc tgaaaactgt aactagtacc
      481 atttcgataa acttcctctt cgacaggctc agagtgacat ttctcctgtc atcctgagct
      541 tgtcgaagga tgagcttgtc cattcttgtc atcctgagct tgtctgttcc tgtcatcctg
      601 agcttgtctg ttcctgtcat cctgagcttg tcgaaggatg agcttgtcga aggatgacac
      661 aaacgaaggg cttagggtaa acgatcctgt ccctcttcga caggctcaga gtgacattat
      721 ggctcagagt gacatttctg gtttcctatc tcctttcgct cttttttctc agcctcagcc
      781 tttttaccaa tgaccaatga ccattctcag ccactcttta tttttccgtg ttcccgccct
      841 gataggtaaa aatatttctg gacagagaag tttctatgtt ctaattttca gctagtttta
      901 aaaattgcag agaattgaaa gaatgatcaa aaatatccac cgaataatcg gcttgctttg
      961 cctaataatt tggatagatt gcgaaggaag cgactatcgg gcattaatcg aaaccggcga
     1021 gtttaaaaaa gccgaggaaa tgattcgccg ggaattattt agtaatcagt ctctcagtgt
     1081 tagtgaaaga cgaacgctgg aatttgaact agaaagaatg agacgcattc gcctggattt
     1141 taataagagt caggctgaag ttatcgaata catcaaacag tacgttcctg atgtgacgcc
     1201 cactgatttg gttcgttggg aatcggaaaa gtcgttggaa tacctcgtga ttgacggcga
     1261 aaaaatgtat ttcaaggatg ccgcgccgaa tcttttccga nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn aataccaatc aaacccttca acggacgatt tacttcgaga
     1441 aagcggcagt caaggattcg actacccatt tttcagtcga atacacttac gaaatttatg
     1501 gttcatatac tccagtaaga gccgagaaag taattccggt agaaccgaat cccgacctga
     1561 atccattcct gagtgaggag ccgccgcata tcgtatttac ggatgaaatc cggcaacttt
     1621 cccagagaat cgtcggttcg gagaccaatc cttatcgtat tgctcagaaa ttatttgagt
     1681 ggattgacgt caatatagtt tgggcttcgg cgcgcgaata ctcgacgatt tataatattc
     1741 ctcaatacgt tataaataat cgccatggcg attgcggtat gatggtgttg acttttatca
     1801 ctctttgccg cctgaatgga attccggcgc gctggcagtc gggttgggaa ttcaaacctc
     1861 ctcaggatag tatgcacgat tggggcgaaa tttaccttgc gccttacggt tgggtgccga
     1921 tggatgtaac ttatggtcgg cgcgccacgg ccgatgaatc tttccgatgg ttttatttga
     1981 gcgggatgga cagctaccgc ctgattttta atgacggaat ggcggctgat ttttatccac
     2041 tgaagattta tccgcgcagt gaaaccattg actctcagcg gggagaagtc gaatgggaag
     2101 gcggcaattt atacttcaat caatggaatt gggaacatga ttgggagatc atttctgaac
     2161 cgtaaatttt ctaatgaaaa ccagcggtga aaattaatac taaaaaaata ctaattgggc
     2221 tgggaattgc actaataata acgttattaa gcggtttgct ttatcataac ggtttgattc
     2281 agcgctggga aaatcaagta aatgatattt tatatcgcga ttttcaggtt gaacaacatc
     2341 cgtcggaggc ggtaattatt gccattgacc aaaatagtct cgattatttt cagaaaaatt
     2401 tccagattct ttggccttgg ccccgcgata tttacgctat ggcgactaac tatttgaatt
     2461 cctgcggcgc aaaagtcatt gtctatgata ttattttttc cacgcctgat attgaccggc
     2521 tgaatgttca agctgaatat gctgattcag cttttgcgcg ggcaatgctg tccagcggca
     2581 aggtcattct ggcaatgcaa atggaagatt cgacgcatct tagtgttgaa aaactgccga
     2641 cccaatttaa tcgccaaatc gtttacaatg tcccccagcg aattgtttac aaatatccga
     2701 atgttacggc tccgattgag cgatttcaaa aagcgatggc gtatcccggc gcggtcaatt
     2761 ttttcaccga taatgatgga gtctgtcgcc gggttccgct tttctttgct aaggatcatc
     2821 aaatcgtgcc ctatatggcg ctcgcggctg tgatgattta cgatgacatt tcctctattg
     2881 attatgattc gatgaggcat actatcttcg ccggaaaacg gcgtgttcct cttaataata
     2941 gtggtttttt cgatatttat tggtatggac caggcggagc aggaaatact tttcaatatg
     3001 tatcttttgc ccagttgttg aattcctatt tgcaagtgca aaatggtcaa aagccagacg
     3061 ttgatccggc gattttccag gacaaagcgg tttttatcgg cgctactgcg gcgggactac
     3121 tcgatctgaa gaccacacca ttttcgtcgg tcgagccata tccgggagtc gagatttatg
     3181 ctactatttt ctcgaatatt atccggggcg attatatttc caatttacca tttataatct
     3241 ggctgggatt ttctctaatt ctaatctggc tgttggcgtt tctttggcaa aagttgaaaa
     3301 tctggcagtc agctgggatt tcgttgataa ttttatttat tccgctgata atgagtgtaa
     3361 tgattttcca gcgcttgaaa atgtttatgc cggttgtaac caccgaaatt gcttttgtgc
     3421 tgagtattac ggtagttcta ttagtgaatt atttgaccga agggcgcgaa aagaaaatga
     3481 ttaagaaagt tttcaatcgc tatttgcatc ccaccgtagt tgaggttttg actaagcatc
     3541 cggaacgttt tgagatgggc ggcaaggaaa tcgtcgctac ggtaatgttc tctgatttgc
     3601 agaattttac tggaattgca gaactgttta ctccacctga aattgtcggc tttttgaacc
     3661 aatattttac caaagtcgaa caaataattt tcaagaacga cggaatgctc gataaataca
     3721 ctggtgacgg aattatggca attttcgggg caccgattcc gactaacgaa cacgcccgga
     3781 tggcttgtaa tgcggcgctg gattttaaaa atttgtcaac ttttacaatt gaagtcaaga
     3841 accggactat cccattaatc acgcgattgg ggattaatag cggtaatttt gtcgtcggca
     3901 atatcggttc ggcgaaccgt atggattata ctgctatcgg cgatacggtt aatctcgcgg
     3961 cgcgtctgga aggcgtcaac aaaatctacg gcacccaaaa tattatttcc gagacgacct
     4021 attccttggt aaaggaaaat tttatctgcc gtgaacttga tttaattcgg gtgaaaggcc
     4081 gtgaaaagcc gctggcaatt tataacatca tctgtattaa agacgattta agttctgaaa
     4141 tagagaagat gctcaaattg tataatgaag ccttacgact ttatcgggaa tgcgaattcc
     4201 agaccgccga acgggcattt ttagaattga gccgtcaatt tccagatgat tcggtcggta
     4261 aagttttcgc caaacgctgt gttcaattaa ttaacgaacc gcagttgatt gacgaagacg
     4321 gtgtatttaa tattaccgtc aagtgaaaca agttaaatta ttgagcaaaa tgaaatagaa
     4381 tttcgaaagt ataaattaaa gcgagaaggg caatatgaaa cgttttattc ttataatttt
     4441 agtagtgtcg gtgttaattg ttaccgtttt tgctgaagaa tccgtggtaa tcaaacgtga
     4501 gaatactcat ttccgacaag gccccggttc ctattatcca ctgattggga tgttgcagaa
     4561 aggcgtccaa gttgctgctg taggaactca atcgggctgg ttgaaagttc agtgggctgg
     4621 gcaaaccggc tggatttccg aaaatgcggt tattgcgagt gatgaaccaa ccggtagcat
     4681 tttgaccggt tcgattagtg gaaatggttc aatgatgatt tcgagagctt ccgccagtgg
     4741 agcggtcaaa ggtttcgccc agaagttcgt caattttcac gacggcgatc cggcttttat
     4801 cgaacaatat gatgttgaat tgtttactcc ggctgaattc cagcgtttta gacaagagac
     4861 ttttcgcggt cgcgacccgg agaaaatccg caaacgctat aagagaatca aatcggaaaa
     4921 caccgatcac tcaatctcat tgcaagatga aaaaattgga ttggcggccg cttccagaat
     4981 tgcagcaagc ggattggttg ccaatcaatc gcaattgaaa tatatcaatc tggtaggcaa
     5041 tatcgttctg gaaaataccg atatgtacga ttatcccttt aaattttata ttctaaaaga
     5101 cagccatccg tcagcctatg ccattcccaa cggtatgatt tttgtcactt ccggtttgct
     5161 cgagctgtta caggatgaag ccgagctggc ggtcatactg gctcatgaaa ttagtcacgt
     5221 tgttcaaaaa catggcgctg aggaaattgc caaacgcaaa acgatgattg tagctgacga
     5281 gggatttaat gaattagacc aagagattgt caccgaagac gctaccgccg atgaactcga
     5341 tctaattgcc ctgaattgct atgagacggc aacaaaacgc cgccaaattg aatatgagat
     5401 cgaagccgat caactcggaa tgatctacgc ctatcgttcc ggttatgatc cgtctgcgct
     5461 gtggcgggtg ctgacgcgca tcgaagccaa cacatcaccg gatttctgga atccagaatc
     5521 caactggagc tatgacgcgg tctttgatcg ggctgagcg
//
LOCUS       DAOSMP010000084         5548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_14783_length_5548_cov_10.887311, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000084 DAOSMP010000000
VERSION     DAOSMP010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14783_length_5548_cov_10.887311"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PKX09_09435"
     CDS             <1..950
                     /locus_tag="PKX09_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU18081.1"
                     /translation="MQIVAEYNFTPSAYGKGLKKQKTENIGVIFCRETRPLSANPFYS
                     RVLEGIEAELVINNYNLVLHLLPEVYRGEYPKMLKEHTVDGIILAGVLDQDFVDLVKE
                     SGIPIVFVNPNFEIEGEDQIFIDDEHGAFQAIQYVISKGHQRIGFVSGDLNRISFKKR
                     YDGYKKALAYHRIEYDDEIVRTGGLENGYDQVTALMKLKNKPTVIFCTNDLNAIFGYK
                     AIEDLGYSIPQDISIIGFDDIDLAKISNPPLTTVRVYKEEMGSIAVRILLEKINKTEH
                     KPVTTLLPVRIIERDSVRDLNNPKSTVRHIEPKQDVELV"
     gene            947..3886
                     /locus_tag="PKX09_09440"
     CDS             947..3886
                     /locus_tag="PKX09_09440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU18082.1"
                     /translation="MKRYLKPAFSALVVMLFINLLNAGVIKGRVIDSKTSDPLIGANL
                     ILAETAGIGAASDYKGYYRIDNVPAGEYTLKVTFIGYQPVEIKISLKADETIVRDIEM
                     VYGGALKGEEVVVTAQAKGQMSAINQQLASNVITNIVDESRIQELPDVNAAESIGRLP
                     GVSIQRSGGEANKVEVRGLNPKYSLITVNGVELPATGSEDRSVNLSLISSNMLDGIVL
                     KKTNTPDMDADVLGGTIDLRLKEAPEGFKFNLSAQGGYNQLQNYYGNYNFTASVSNRF
                     LDNRLGIVVNANTDNYDRSADKLRDNWRNYNKMLATGELIMIEEIVNRKRTGASLLLD
                     YNLPKGKITANGFYNQLKSDGLIRNNQVNTLELGYSTNRHLYKVEEEKSTTNVFTSAL
                     SINQDFRWLKFDASISRSGTITDSPDRRVTEFTQEQSALLPGYPNFVDPIELVPFFNV
                     DTIITNMSAMYSYGKKIVENTTSAKLNIELPFRIGSLLNGSFKTGGKLRWIDRSNDEN
                     QHGYSGLQYGSGQQNPVFLYVDRVYPEWGIMKNVEEYQLLNVLPFLKDYSRPDFLNGD
                     FPLGLTFDEKKLRKMSDALIAAPDSLNLWVPYSVGTFGYDYDGFERYQAFYFMGELNI
                     GNYLTVIPGVRYEGDYSKYHGQRYMVNQSGQTVEQPPSEFVRLTKERENEFWLPMVNI
                     VAKPTNWLNIRLARTETIARPDFLRYAPISYISSDRKSVNAANYSLKPSKSTNYDVGI
                     SLFNNEIGLLSATAFHKNIEDLIFYSTLNYRPGVEIDSALNIPASWLKSNPQINAYRN
                     NPDPAKYYGYEIEWQTHFWYLPSVFRGIVLNINYTHIYSEMKLVYDSLVTKQVGTRRV
                     YSLVPAYIKTRMPDQPAHVFNITIGYDLKGFSARLSYLFQTDKLTGIGYDGVIPTTRL
                     SSYTSDYGRWDLTLQQKLFQNVQLFVNLNNLNNRYDQSCIGADLTHPSYIEYYGFTMD
                     LGIRVNL"
     gene            4084..>5548
                     /locus_tag="PKX09_09445"
     CDS             4084..>5548
                     /locus_tag="PKX09_09445"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOU18083.1"
                     /translation="MKSKNLVVWFTMLAVVLVMSAGQILAQTEDTLVVNWDDGAGNIV
                     QDALYNAVMGDTAADGSRANLNRVYKLKAGGYYWNNKTISNDFPLRLVGEVPTGDEHP
                     AVIQMVTDAESGSTPGKMISCASDLTLKNLYIIGSDELGSSGYYQPIEVNAVAKRFII
                     DNCVFERSNFAIVAFAGGKDNDLFITNCVYRNLQEDVPTQQWCGRGLSVWTDIDTVVI
                     ENNTFFNINMCAIQIENGAANYLRFNHNTLVNIGRAFTSTTNVWWREAYFTNLLFVNT
                     WWHGEGYCDYSIQYQPGRDPRAYTTGQFPVLDMPSRYGTNKGRRILFSNTAAYLDPFF
                     TTHYGDTIRTQPYVNAITDSFFTTFSPDNGGQMVIKDTTWLSAKPTFSSDPNTPAQLE
                     AMWNHITASRGYQYYGTGIQAKPYYYGLVTEGTDTLWAEPAWPLPENFTYTDDLTDLS
                     TDGLPLGDLRWFPAAKTTFEANKDSYVNAIEWMPGERI"
BASE COUNT         1710 a          986 c         1227 g         1625 t
ORIGIN      
        1 ttatgcaaat agttgctgaa tataacttta ctcccagtgc ttacggtaaa ggcttaaaga
       61 aacagaaaac cgagaacatt ggcgtgatat tctgtcggga aacgcgcccg ctatcagcaa
      121 atccatttta ttcacgtgta ctggaaggca tcgaagctga actggtcatt aataactata
      181 atttagtgtt gcatttactg ccagaagtat atcgcggtga atacccgaaa atgctcaaag
      241 agcataccgt ggatgggata atactggcag gggtacttga tcaggatttt gtggatctgg
      301 ttaaagaatc cggaattccc attgttttcg ttaatcccaa tttcgaaatc gaaggagaag
      361 atcaaatttt catagatgat gagcatggtg cttttcaagc gattcaatat gtcataagta
      421 aagggcatca gagaattggt tttgtttccg gtgatttgaa tagaattagt tttaagaaac
      481 gatacgatgg atataaaaag gcattggcct accatagaat tgaatatgat gatgaaattg
      541 tccggacagg aggtttggaa aacggatatg atcaagttac tgctttgatg aaattgaaaa
      601 ataaaccgac ggtaattttc tgcacaaatg accttaatgc tatatttggc tataaagcga
      661 ttgaagactt agggtatagt attccacagg atataagtat aattggattt gatgatattg
      721 atctggcaaa aatatcaaat ccgccattga caactgtgag agtttataaa gaagagatgg
      781 gatcaatcgc ggttagaata ttattggaga aaattaacaa aaccgagcat aagcctgtta
      841 ctactctttt accggtaaga ataattgagc gtgattcggt aagagatctt aacaatccta
      901 aaagtaccgt tagacatata gaacccaagc aagatgtgga gttagtatga agagatattt
      961 aaagcccgca tttagtgcat tagtagtaat gttgtttatt aaccttttga acgcaggagt
     1021 aataaaaggg agggtaatag attcaaaaac cagcgatccg ctcattggtg cgaatctgat
     1081 tttagctgaa acagccggta ttggagccgc ttccgattat aaggggtatt atagaataga
     1141 taatgtaccg gccggcgaat ataccttgaa ggtgactttt attggttatc agccggtgga
     1201 aataaaaatc agcctgaaag cagacgaaac catcgttcgt gatatcgaaa tggtctatgg
     1261 cggggcgcta aaaggagaag aagttgtcgt caccgcgcaa gccaaaggtc agatgtcggc
     1321 tatcaaccaa cagttagcct cgaatgtaat taccaatatt gttgatgaga gccgtatcca
     1381 ggaattaccg gatgttaatg cggctgagtc aatcgggcgt ttacccggtg tttccattca
     1441 gcgttccggt ggtgaagcca ataaagttga agttcgaggt cttaatccca aatatagttt
     1501 gattacggtc aacggagtcg aacttccagc caccggttcc gaggaccgaa gtgtcaacct
     1561 ttcactaatt tcgtccaata tgctggatgg gatcgttttg aaaaaaacca atactccaga
     1621 tatggatgct gatgtattag gcggcacgat tgatttaaga ctaaaagaag cgccggaagg
     1681 ttttaagttt aacttgtcag ctcaaggtgg atacaatcaa cttcagaatt attacggcaa
     1741 ttataatttt accgccagtg tcagcaatag attcctagat aatcgtctcg gaatagtagt
     1801 aaacgcaaat acggataact atgaccgtag cgccgacaaa ctgcgagata attggcggaa
     1861 ttataataaa atgcttgcta ctggtgaatt aatcatgatt gaagaaatcg tgaatcggaa
     1921 acggaccggg gcgagcttat tattagacta taatctacca aaagggaaaa ttaccgctaa
     1981 tggtttttat aatcagctta aatccgatgg cttgattcgt aacaatcagg ttaatacctt
     2041 ggagcttggt tatagtacca atcgccattt atacaaagtt gaagaagaaa aaagtactac
     2101 caacgttttt acgagtgcgc ttagtattaa tcaggacttt agatggttga aattcgatgc
     2161 cagtataagc cggtcgggta ccattaccga cagtccggat cgccgggtaa ccgaatttac
     2221 acaggaacaa agtgcattat tacctggcta tcctaacttt gtcgatccaa tcgaacttgt
     2281 cccgtttttc aatgtcgata caatcattac caatatgtcg gctatgtaca gttatggaaa
     2341 aaagattgtt gaaaatacaa catccgccaa attgaatatt gaattgccgt ttcgtatagg
     2401 ctctctttta aacggtagtt ttaaaacggg tggaaagtta cgctggattg atcgcagtaa
     2461 tgatgaaaat caacacggat atagtggatt acaatacggg tcaggccaac aaaatccggt
     2521 atttttatat gttgatcgag tctatccaga atggggcatt atgaaaaacg ttgaggaata
     2581 tcagttgcta aatgtgttgc catttttgaa agattactct cgtcctgatt tcttgaatgg
     2641 tgattttcca cttggattga cttttgatga aaaaaagttg cgtaagatgt ccgatgcttt
     2701 gatagcggct ccggatagtc tgaatttatg ggtgccctac tcagtcggga cattcggata
     2761 tgattatgat ggttttgaac gctatcaggc tttctatttt atgggcgagt taaatattgg
     2821 taactattta accgtgattc ctggtgtacg ttatgaaggg gattattcaa agtaccatgg
     2881 tcaacgctat atggtgaacc aatccggtca gacagtagaa cagccgcctt ctgaatttgt
     2941 taggcttact aaagaaagag agaatgaatt ttggttgccg atggtaaata ttgttgcaaa
     3001 gccaaccaat tggttaaata ttagattggc acggacggaa accattgccc gcccggattt
     3061 cctaagatat gcgccgattt cgtacatttc ttcggataga aaatcggtca acgcggccaa
     3121 ttattctttg aaaccatcca aatcgacgaa ttatgatgtt ggcatttctt tatttaataa
     3181 tgagattggt ttactttcag cgactgcttt tcataaaaat atcgaggacc ttatttttta
     3241 ttccacatta aattaccgtc cgggagtcga gatcgattcg gcgttaaata tacctgcgag
     3301 ttggctaaaa tcgaatccgc aaattaatgc ttatagaaat aatcctgatc ctgccaaata
     3361 ttacggttat gaaatagaat ggcagactca cttctggtac ttaccgtcgg tatttagggg
     3421 aatcgtttta aatattaatt atacccacat atactcagaa atgaaattag tatatgatag
     3481 tttagtaacc aaacaagttg gaacaaggcg agtctattcg ttggtgccag cttatattaa
     3541 gactcgtatg cctgatcaac cggcccatgt ttttaatatc acgatcggat atgatttaaa
     3601 aggattctca gctcgtctct cttatttatt ccagactgat aaattaactg gtattggata
     3661 tgacggcgta attcccacga cgagattaag tagttatact agtgattatg ggcgctggga
     3721 tttaaccttg cagcagaaac tctttcaaaa tgttcaattg ttcgtcaatt taaataattt
     3781 aaataatagg tatgaccaaa gttgtattgg agcagatttg actcatccga gctatatcga
     3841 gtattacgga tttactatgg atttaggtat tagagtcaat ctttagagga atatttaatg
     3901 ttgattttaa taaatagggg gaaatcaaat gatgttagca attcaaatcc acccgcctta
     3961 agagcgttat ttaaactaaa accattaaaa aatgaaagga gaaagtgcca aaaaatgatt
     4021 ttaagagaaa aattgctatt aaattgccaa accaagaaaa ttaatcaaaa ggaggattat
     4081 ttaatgaagt ccaaaaactt agtagtttgg tttactatgc tggcagtcgt gttggttatg
     4141 tcggctggcc agattttagc gcagactgaa gatacgctgg ttgtaaattg ggatgacggc
     4201 gccggcaata ttgtccagga tgctctttac aatgccgtta tgggagatac ggcggctgat
     4261 ggtagccgtg ccaatctgaa tcgggtctat aagctgaaag ccggtggtta ttactggaat
     4321 aataaaacca ttagtaatga cttccctctg cgtttagtag gagaagtacc cacaggtgac
     4381 gaacatccgg ctgtaattca gatggtcaca gatgcagaga gtggaagtac accaggtaaa
     4441 atgatttcct gtgccagtga tcttacactg aagaatcttt atattattgg ttctgatgag
     4501 ctgggctctt cagggtatta tcaaccaatt gaagttaacg ctgttgcgaa aaggttcatt
     4561 attgacaatt gtgtgttcga acgttcaaat tttgcgattg ttgctttcgc aggcggcaag
     4621 gataatgacc tctttattac caattgcgtt taccgcaatt tacaggaaga tgtgccaact
     4681 caacagtggt gcggtcgcgg actctcagta tggaccgata ttgatacagt agtcattgaa
     4741 aataatacct ttttcaacat taacatgtgc gctatccaga ttgagaatgg tgccgcaaat
     4801 tatctgcgct tcaatcataa taccttggtt aatattggtc gtgccttcac ttcaactacc
     4861 aatgtatggt ggagagaagc ttacttcacg aatttgctgt ttgtcaatac ctggtggcat
     4921 ggtgaaggat attgtgatta tagtatacaa tatcaaccag gtcgtgaccc aagagcatat
     4981 accacgggtc aattccctgt tcttgatatg ccgtcaagat atggaacaaa caaaggtcgc
     5041 cgtattctat tctccaatac cgccgcctat ctggatcctt ttttcacgac tcattatggg
     5101 gatacgatca ggactcagcc ttatgtgaat gctatcacgg attcgttttt tacaacattc
     5161 agtccggaca atggcggtca gatggttatt aaagatacga cttggcttag tgcaaaacca
     5221 acattcagca gtgatcctaa tactccggct caattagagg cgatgtggaa tcacatcaca
     5281 gcttcgagag gttatcagta ttacggaacc ggaattcagg ccaaacctta ctattacgga
     5341 ttagtaactg agggcacaga tactctctgg gcagaacctg cctggcctct gcctgagaac
     5401 ttcacctata ccgatgattt gactgattta agtaccgatg gtctgccgct gggtgatttg
     5461 cgctggttcc cagccgctaa aacgaccttt gaggcgaata aagattccta cgtaaatgct
     5521 atcgaatgga tgccgggtga aagaatca
//
LOCUS       DAOSMP010000085         5407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_15244_length_5407_cov_17.476644, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000085 DAOSMP010000000
VERSION     DAOSMP010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15244_length_5407_cov_17.476644"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PKX09_09450"
     CDS             complement(<1..401)
                     /locus_tag="PKX09_09450"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOU18084.1"
                     /translation="MVNESSIASAGLVPIRQIAEKIGIPPEAMDLYGDYKAKIKLSYF
                     NQLPPNAKNKLILVSAMTPTPAGEGKTTVAISLAMGLNRIGKSAIVTLREPSLGPLFG
                     MKGGATGAGKAKVLPVEEINIHFTGDIHAVTA"
     gene            complement(445..996)
                     /locus_tag="PKX09_09455"
     CDS             complement(445..996)
                     /locus_tag="PKX09_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU18085.1"
                     /translation="MLRDILDRRRATRSLAPVEITQAMIKDLAESARLMPSCFNKQPW
                     RFIFVYDKEQLTRLHSALNKGNEWAKKASLIVAVFTKKDLDCVIKDREYYLFDTGMAT
                     AALILRATEMGLVAHPIAGYDSDKVKEALNIPAEYQVIALINIGKQADEIDPILSDYQ
                     KLAEKERPARLPLENFVYLNKFS"
     gene            1097..2239
                     /locus_tag="PKX09_09460"
     CDS             1097..2239
                     /locus_tag="PKX09_09460"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU18086.1"
                     /translation="MIDKPKILTLLIVYFSLISLFSKPGNADEWQEQKIQQALFEMYN
                     LNNVVAHQHFKEVLNSQPFHPLAPMAELATDWLKNQEELGYQEGNRILASRIDSVLTI
                     YHDKLKANPQNGELEFYYGCTYGLKARLSLGEKDYLGVLINGYNAVRYIKAAERHQPD
                     FIELQIPFGVFNYYVGISRGYMQIASWIMNTSGSKEEGLAQMRYAAHNARYSCYEARS
                     ILAMVTLYFEGDYKSTLYYAEMMASDFPNNPYYNYLAGEALIQMGRFAEAEKQIKRIK
                     QLLPKLKPKTQKEYWSKLHQLQGSLALKKGDLATAEKELRAVIDNYDQEMDVQLGFAL
                     LRLGNVYDLTGRRELAVQCYQRAYGLKNRSQACQLAKLYLKTPYTQ"
     gene            2566..2730
                     /locus_tag="PKX09_09465"
     CDS             2566..2730
                     /locus_tag="PKX09_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18087.1"
                     /translation="MTALKIPFFPLGLRFPAGDVLKAVMVNWMVSFSAPLLLFSQSLF
                     STFLLFINYQ"
     gene            complement(3026..>5407)
                     /locus_tag="PKX09_09470"
     CDS             complement(3026..>5407)
                     /locus_tag="PKX09_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOU18088.1"
                     /translation="TAKLWEVATGKEIRTFRGHNDWVYSVAFSPDGKYIATGSNDKTA
                     KLWEVATGKQIRTFRGHNDWVYSVAFSSDGKYLATGSRDCTAKVLGFHIEIILGIISN
                     HNVILDSLSSEHEKKLAELFAPRGEFETTQQYQQRLKNDEQQKAMLTNLYQQKRQFYD
                     QQIQSEIAQSRQKVTLPNSGTLGIYNPDKQVFPFEYKNQTYLMNVPLNQAPDFKNRFE
                     TFIIEGERQLNAQGDWEYFNYALVDPQTGARYLFGEQKGIPVIAIAQKPTFPPRLQAN
                     LSFTEPSGEGFLDAGEKGTVMVEISNSGQGDAFNIIAELKSMSASSGIYFEIMKSIAQ
                     IKPNEKKSLIFTLEASDNLPTGTLNFEVKFSEANGFEPEPINFSLETHALRPPEIVIA
                     DFAVRDQSGNGKIEQTEPATLTLRLHNRGTGTSHASKAVVQFGANVFPFADSPTEFSL
                     GNIPPGGIKDVEFTFYTNNRVQDKIPINLRIDDARPQFQKTLALDLQVNVPQKSLEQL
                     TFKGTEKVSVATETPSLSVDIEQNIPQVQISKPDAIAVVIGNRDYTRVKNVEFAIRDA
                     EYVKDYLVKMLGYKEGNILFIKNASKADFEACFGTKDNPRGRLADLVKSGKSEVFIYY
                     SGHGAPGLKTHEGYFVPVDCDPQRVELTGYPLDVFYNNLAQIPAQNFTVVIDACFSGT
                     NIYENISPIMIEISAPEAVLPNGIVFTSAGPQQVSAWYNEKFHSLFTYFFLKGLHNYN
                     ADLNKDRQITYRELYQYLNDQNEGVPYWARRLHGVEQTPVFFGTRQDEVLIKY"
BASE COUNT         1485 a         1203 c         1173 g         1546 t
ORIGIN      
        1 gcagttacgg catgtatatc acccgtaaaa tgaatattga tctcttcgac tggaagtact
       61 ttggctttgc cggcgccggt cgcgccgccc ttcataccaa aaagtggtcc caaggatggt
      121 tccctcagtg taacaatcgc acttttacca atgcggttca atcccattgc cagactgatt
      181 gctacggttg tcttgccttc accagccggg gttggcgtca tagccgagac cagaattaat
      241 ttatttttag cgttgggagg gagctgattg aagtaactca gcttgatttt ggctttgtaa
      301 tcaccatata aatccattgc ttcgggaggg attccaattt tttccgcaat ttggcgaatt
      361 gggacaagac ctgctgaagc gatcgaagat tcattgacca taacgcgcct ttattagtta
      421 attaatttaa tcaacggcta tatgttaact aaatttattt aaataaacaa aattttcgag
      481 aggcaagcgc gccggtcgtt ctttttcggc taatttttga taatcgctta aaattggatc
      541 aatttcatca gcctgtttac cgatatttat gagagcaatt acctggtatt cagcgggaat
      601 attcaaagcc tccttgactt tatctgaatc gtaaccggca atgggatggg cgaccaatcc
      661 catttcggtt gcgcgcagga tgagagccgc agtcgccatt ccagtgtcga atagataata
      721 ttctcgatct ttgataacgc agtcgaggtc ttttttagtg aaaaccgcca ctatcagcga
      781 agccttttta gcccattcat tgcctttgtt aagagctgaa tgcaggcggg tcaactgctc
      841 tttatcataa acaaaaatga atcgccaagg ctgtttatta aaacaagacg gcattaaccg
      901 ggcactttcc gctaaatcct ttatcatcgc ctgggtgatt tcaactggcg ctagtgagcg
      961 cgtggcgcgg cgtctatcta aaatgtctct cagcataaca aacctcccta ttttaatctt
     1021 ttgtattttc acttgttttg cacgcctttc aatggttacc tttagagaag ataatgaaat
     1081 aaattgttac atagcaatga tcgataaacc caaaatttta acgcttttaa ttgtttattt
     1141 ttctttaatc agtttattct caaaaccggg taatgccgac gaatggcagg aacaaaaaat
     1201 ccaacaagcc ttatttgaga tgtataacct gaataacgtc gttgcgcacc agcattttaa
     1261 agaagtatta aatagtcaac cgtttcatcc gttggcgccg atggcagaac tggcaactga
     1321 ttggttgaag aatcaggaag aattgggcta tcaggagggc aatcgaatcc tggcttcgcg
     1381 gattgactcc gttctgacaa tttatcatga taaattaaaa gctaacccgc agaacggcga
     1441 gctggaattt tattatggtt gtacttacgg tttgaaagcc cggttgtcgc tcggtgaaaa
     1501 ggattacctg ggtgtcttga ttaacggcta caatgcggta cgctatatca aggcggcgga
     1561 acgtcatcaa ccggatttca ttgagctcca aatacctttt ggcgtgttca attattatgt
     1621 gggtatttcg cgaggctata tgcaaatcgc ctcctggatt atgaatacgt cgggaagtaa
     1681 agaggaggga ttggcgcaga tgcgttatgc cgctcataat gcgcgttaca gttgttacga
     1741 agcgcgcagt attctggcaa tggtaacttt atatttcgaa ggtgactaca agtccacact
     1801 atactatgcg gagatgatgg cgtccgactt tcccaataat ccgtattata attacctagc
     1861 tggtgaggcg ctgattcaaa tggggcgctt cgccgaggct gaaaaacaga ttaagcgaat
     1921 caaacaatta ttgccgaaat taaagcccaa gacgcaaaag gagtattggt caaaattgca
     1981 ccaattgcag ggcagtctgg cgcttaagaa aggtgatctg gcgacagctg agaaggaact
     2041 tcgggcagtg attgataatt atgaccagga aatggatgtc cagttgggat ttgcgcttct
     2101 tagattgggc aatgtctatg acttgaccgg tcggcgggaa ttggcggttc aatgctacca
     2161 gcgagcgtat ggattgaaga accgctcgca agcctgccaa ttagccaagc tttatttgaa
     2221 aacaccctat actcaataaa taggctgagg ctgagaaaag aggctgaggc tgagaaaaga
     2281 ggctgagact gagaaagaac ttaaatctca atctcttttc tcaacctttt tactaattac
     2341 taattaccaa tgaccaatga ccaatgacca atgaccaatg actaatgaca aactttcctg
     2401 ttcttttttc ctttgccgaa aaaagaacca aaaaaggcaa cggctaaaga acatctcacg
     2461 ggggttcgct cagtcccagc gaatggaatt taaggcgcaa aagttatcaa gttcatataa
     2521 tgttagtgac atagtctaaa agtgatagat aattacgaaa attctatgac agcgctgaaa
     2581 attccatttt ttccgctggg acttcgcttc ccagccggag atgttcttaa ggccgtaatg
     2641 gtaaactgga tggttagttt tagcgctcct ctgctcctct tttctcagtc tcttttctca
     2701 acctttttac tatttatcaa ttaccaatga ccaatgacta atgaccaatg accattctta
     2761 accttatttt tcttgttttg gtcttaagag aataaaccgc caagttcgcc aagaaccgct
     2821 aaatgagtaa aatcccaaac tccaaattcc aaatcacaaa tagccaaacc aatttccgcg
     2881 aaactgtctc ctgccttcct tctctatctc cctttctcag cctctatttt cttattctct
     2941 ttactcagtc tctttactaa ttaccaatga ccagtgacta atgaccaatg accaatgacc
     3001 aatgaccaat gacagcctgt tttctctaat acttgatcag cacttcatcc tgccgggtac
     3061 caaaaaacac cggcgtctgc tctactccgt gcaggcgtcg cgcccaataa gggacgcctt
     3121 cattctggtc gttgagatat tgatacaatt cccgataggt aatttgccgg tctttattca
     3181 aatcggcatt ataattatgt aagcctttta gaaaaaagta agtgaacagg ctatggaatt
     3241 tttcattgta ccaggcggaa acctgttgcg gtccagcact ggtaaataca attccattcg
     3301 gcaagaccgc ctccggcgct gagatttcaa tcattatcgg actgatattc tcgtagatat
     3361 ttgtaccgga gaaacaagcg tcaatgacaa cggtaaaatt ctgggcagga atttgcgcca
     3421 gattattata aaaaacatcc agcggatagc cagtcagttc tacccgctga gggtcacaat
     3481 ccactggtac aaaatatcct tcgtgcgttt ttaagcccgg cgcaccgtgc ccggaatagt
     3541 aaatgaaaac ctcgcttttg ccgctcttta ccaaatctgc cagcctccct cgcggattat
     3601 ctttagtacc aaagcaagcc tcaaaatcag ccttactcgc atttttgata aacaagatat
     3661 tgccttcttt atagccgagc attttgacca aataatcctt gacatattca gcatcacgga
     3721 tagcaaactc gacattcttc actcgcgtat aatcacgatt gccaatcacc acggcaatgg
     3781 catccggctt agatatttgc acttgcggaa tattctgctc aatgtcaacc gaaaggctcg
     3841 gcgtttctgt agcaacggaa accttttccg tgcctttgaa ggtcaattgc tctaaacttt
     3901 tctgaggaac attgacctgc agatcaagtg ccaacgtttt ctgaaattga ggtcgggcgt
     3961 catcaatcct caaattaatg ggaatcttat cctgaacgcg gttattggta tagaaagtaa
     4021 attcaacatc tttgataccg cctggtggaa tattaccgag tgaaaattcg gttggtgaat
     4081 cagcgaaggg gaagacatta gcgccaaatt gcacgacggc tttgctggca tgactggtgc
     4141 cggtgccgcg attgtgcaga cgtagcgtca gagtagcagg ttcagtttgt tcgattttgc
     4201 cgttaccgga ttggtcgcgc acggcgaaat cagcgatgac aatctccggc ggtcgcaagg
     4261 catgagtttc gagtgaaaaa ttaatcggct ctggctcaaa gccattggct tcgctgaatt
     4321 tcacctcaaa attgagagtg ccggtgggga gattatcact ggcttcaagg gtgaaaatca
     4381 ggcttttctt ttcattcggc ttgatctggg cgatactttt cataatttca aaataaatac
     4441 cgctactcgc agacatactc ttcaattcgg caatgatatt gaaagcatca ccctgaccgc
     4501 tattgctgat ttcgaccata accgtgccct tttcgccggc atctaaaaaa ccctcgccgg
     4561 aaggttcagt gaaactcaga ttggcttgca ggcgtggcgg gaaagtcggc ttctgggcaa
     4621 tggcgataac gggaatgcct ttctgctcgc cgaacagata gcgcgccccg gtttggggat
     4681 caacgagagc ataattgaaa tactcccaat ctccttgcgc attcagttgt ctttcgcctt
     4741 caattataaa agtttcaaac cgatttttga aatcaggcgc ctgatttaat ggcacattca
     4801 tcaaataagt ttgattttta tattcaaaag ggaatacttg cttatcagga ttatagattc
     4861 ccaatgttcc ggagtttggt aaggtaactt tctggcgaga ttgagcgatt tctgactgga
     4921 tttgctggtc atagaactgc ctcttctgct gataaaggtt tgtcagcatg gctttttgct
     4981 gttcatcatt tttcaggcgt tgctgatact gctgggtggt ttcgaattca ccccgggggg
     5041 caaataactc tgccagtttt ttttcgtgct cactacttaa agagtcaagg ataacattgt
     5101 gattagatat aatgccgaga ataatctcta tatggaatcc caggacttta gcggtacagt
     5161 ccctagatcc ggtcgccaga tacttaccgt cagacgaaaa ggcaacagaa taaacccagt
     5221 cattatgtcc gcggaaggtg cggatttgct tgccggtagc cacctcccag agtttagcgg
     5281 tcttatcatt tgacccagtc gcaatgtact taccgtcagg tgaaaaggca acggaataga
     5341 cccagtcatt atgtccgcga aaggtacgga tctccttgcc ggttgccacc tcccagagtt
     5401 tagcggt
//
LOCUS       DAOSMP010000086         5305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_15629_length_5305_cov_8.061143, whole genome shotgun sequence.
ACCESSION   DAOSMP010000086 DAOSMP010000000
VERSION     DAOSMP010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15629_length_5305_cov_8.061143"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..1201
                     /locus_tag="PKX09_09475"
     CDS             47..1201
                     /locus_tag="PKX09_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18089.1"
                     /translation="MRKSAHFILIIVLTFISVLFFACPDDPEPEPTTCDYPPGNRTFT
                     WRVDTVAYWPSFLGGVHAFADDDAYVMGYIGEGQAPWRIFVGKHWNGTKWDNNIYGTD
                     AEVGHVANDVTGDDHFMVSVGNWSWTPPKPAIGEFDNLAKKWKAYQFETEGELRSVWT
                     DGKGFFIAVGDNGMVYTKDGYKAEWVYSKAPTEFHFTNIVGISKHEMYAISQLPTITG
                     VIYQQYWKCYNNQWIKLYDGQDTTGTPVKLYGTDNSMYDLSVSRCSYNDSLKLYLIGW
                     ESFLLESEGQSLNYSITNLSQMGLPLRSMGRTAVRINLYSPNDYWIFGTRYNFYHWNG
                     TDFQKIVMPGLPDDDAHFGDQHRMIKTSTGKIFLPTEVSSQVYVVVQGTP"
     gene            1282..2469
                     /locus_tag="PKX09_09480"
     CDS             1282..2469
                     /locus_tag="PKX09_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18090.1"
                     /translation="MRKSAHFILIIVLAFISILFLACPDDPEPQPTNCEYPPGNRTFT
                     WRVDTVAWFGTSTVAGVWAFSDNDAYVMGGIIDGKPPYSGRFGRHWNGETWEDDINGT
                     WGYNDVTEEWGDISIGPLGDVTGDDHFMVAVGYWGLNQDIYAGIAEFDNRIKKWKSYQ
                     LQATGELRSVWTDGKGFFIAAGDNGMVYTKDGYKAGWIYSKAPTEFSLFNVRGVSKNE
                     LYFRGDYAAPGIHYTQIWRLYGGAWVKLLDNQDTTNTPIKIPEAGYDIYDIAVSRCSI
                     TDSLILYIIGDESFIFESFGLNLEFTKTNLTYLGLPLKQNGRTGTDINLFTPNDVWIF
                     GTRYNFYHWNGTDFQKIVMPGLPDDDAHFGNQRKMIKTSTGKIFLPTEVSSQVYVVVQ
                     GTP"
     gene            2503..>5305
                     /locus_tag="PKX09_09485"
     CDS             2503..>5305
                     /locus_tag="PKX09_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18091.1"
                     /translation="MKTKTWKKMISSVFIVLICLQIGFADDVRLKIPESTVNNILAAI
                     VESKYLSYGTSCNIDISFLSLNVAVVTYYSFKPQDISLDISTGNNFSLTIDFKIKANF
                     DTPIFDFLLKASNLSVTINGTIDRVEQGSGYKIRLTPLSLYYNNNDWVNIVLHDQLSG
                     LIAKLPEISTSSSQPLLPNIVASYFTSGTPTLTTTDTSIVLGLTMVAGLRNIKVQNEV
                     NQLNTLGNVQTVVNGSVTGTYPSPKTFEWNTGNVKQVQTPNELIASTNGQNKYKNWYK
                     DDATLPETDIATRRIEITVASSDATYTAKFLQAQRVQLSNVLEGSLTGGTVTYESTTG
                     SMYDSYEYCYNANPVPHSISTNVPSGTLNSDWVFYHWSDGNTSQTRNIVVDSDKNLQA
                     SWKGIHRSNAATAFSNNSQRKFVRTQDGWLHQVYESMGRVWLEHSSNNGSTWFLGNNG
                     LPLDDGEGKCPSIDWGHYYYSSSSQGTYVHEHIIVVAYQQKFGNYYKIRYAVFKKDNN
                     VYVNYTPSGSYATLYTESSDQYSVDANPNIAISGCSSGIYDFIISFERKSGSMAGINW
                     FYGKMAYIGIYPTYNNYVSPVWITGTNASSINASVHLNKDIVNPRESFDIVYQQGTQY
                     IKDVILSCSYNGVGNWTTYQFGPFTISQGTDRKSYKPSLVQKTNRDISVSWIRDRTAD
                     GGSPYYINAVNWDSENSSVYRIYGSWDVRSVSSNVRDDNTAVYTAWSEAQYIGSSASK
                     NCVARGSSSITLNTHGKYLQLCNGPLSGGSTNMRVSAYDISSSPYFFETSNAIGTSLP
                     KSTVSDINYGRGIALDNGNIGFFYSIKKLSAGKQAIHFIEIPTKPNIIRSVENASIKP
                     LMLDSLNAYLLSEPFSVTEDMEITFVEDGGFTKLLPSTGNVDTLKAVEKGRHIKCKLE
                     LIDDDTQKLVGTIRVTQFNL"
BASE COUNT         1596 a          994 c         1190 g         1525 t
ORIGIN      
        1 ttatttcttt cgctctgtcg cgatatatat ctaatggagc tttaatatga gaaaaagcgc
       61 tcattttata ttgatcatag ttctgacttt catctcggta ttattttttg cctgtccgga
      121 tgatcccgaa cctgagccga cgacctgtga ttatccgccg ggaaaccgca ctttcacctg
      181 gcgggtggat acggtggcgt attggccgtc ttttctgggt ggagtgcatg ccttcgctga
      241 cgatgacgcc tatgtaatgg gatacattgg cgaaggtcag gctccctggc gcatatttgt
      301 tggtaaacac tggaacggaa ctaagtggga taataatatc tatggaacag atgccgaagt
      361 tggtcatgta gctaatgatg taactggcga tgatcatttt atggtatccg taggtaattg
      421 gtcctggaca cctcccaaac ctgcaatcgg agaatttgac aacctggcaa agaaatggaa
      481 agcatatcaa ttcgagacag aaggtgagct acgcagtgtc tggacggacg gcaagggctt
      541 cttcattgcc gtcggagata atggtatggt ctataccaag gatggctata aagcggaatg
      601 ggtgtattcc aaagcgccga cggagtttca ctttacaaat atagttggta tctctaaaca
      661 tgaaatgtac gcaatttctc agcttcccac tatcacagga gtaatctacc agcaatactg
      721 gaagtgttat aataatcaat ggataaaact ttacgatggg caagacacta ccggtacgcc
      781 agtaaagtta tatggtacag acaatagtat gtatgattta tccgtatcgc gttgttctta
      841 taacgattcc ttgaaattgt atttaatcgg atgggagtca ttccttttag aatctgaggg
      901 ccaatccctg aattattcca taacgaatct ttcgcaaatg ggattaccct taaggagcat
      961 gggtagaact gcggtacgga tcaatctcta ttcaccgaat gattactgga tattcggcac
     1021 aaggtataat ttttatcatt ggaatgggac tgatttccag aagatcgtaa tgccgggttt
     1081 gccggatgac gatgctcatt ttggcgacca gcaccggatg atcaagactt ctactggaaa
     1141 gatatttctt ccgacggaag tcagttcgca ggtgtacgtt gtggtacagg gtacaccata
     1201 attatcaatc aattaaaaac catgaaggag tggatatgaa aatgaaaacc tggaagaaga
     1261 tttaaccttg tgtggtgttt aatgagaaaa agcgctcatt ttatattgat catagttctg
     1321 gctttcatct ctatactatt tcttgcctgt ccggacgatc ctgaacctca gcctacaaac
     1381 tgcgaatacc cgcccggcaa ccgtactttt acctggcggg tggatacagt tgcctggttt
     1441 ggtacatcta cggttgctgg ggtatgggcc ttttcggata atgatgcgta tgtaatgggc
     1501 ggtatcattg atggaaagcc accctattcc ggaaggtttg gaagacattg gaatggagaa
     1561 acctgggaag atgacattaa tggaacctgg ggttataacg atgtcactga agagtggggt
     1621 gatatttcaa taggcccgct tggtgatgtc accggcgatg accactttat ggttgcagtc
     1681 ggatactggg gtttaaacca ggatatttat gccggaatag ccgaattcga taaccgcata
     1741 aagaaatgga aaagttatca actacaggcg accggcgaac tgcgcagtgt ctggacggac
     1801 ggcaaaggat tctttatcgc ggcgggtgat aacggcatgg tctataccaa ggatggctat
     1861 aaagcaggat ggatatattc caaagcgcca acggaattta gtctgtttaa tgtccgggga
     1921 gtatcaaaaa acgaactgta tttcagaggt gattatgcag ccccgggaat ccattataca
     1981 cagatatgga ggctttatgg tggcgcttgg gtaaaattat tggacaatca ggatacaacc
     2041 aacacaccaa taaaaattcc agaagccggg tatgacatat acgatatagc agttagccgg
     2101 tgctctatca cagattcgtt gatactctac attattggag atgaatcgtt tatttttgaa
     2161 tcatttggat taaatctcga gttcacgaaa acgaacctta cttatttggg tctgccatta
     2221 aagcaaaatg gacgtaccgg aacagatatt aatcttttca cgcccaatga tgtgtggata
     2281 ttcggtacgc gttataattt ttatcactgg aatggaactg attttcagaa gattgtaatg
     2341 ccgggtttgc cggatgatga tgctcatttt ggcaaccaac gcaaaatgat caagacttct
     2401 actggaaaaa tattccttcc gacggaagtc agctcgcagg tgtacgttgt ggtacagggt
     2461 acaccatgat tattaatcaa ttaaacttat gaaggagtga atatgaaaac gaaaacctgg
     2521 aagaagatga tttcttctgt ttttattgta ttgatctgct tacagatcgg ttttgcggat
     2581 gatgtgcgtc tgaaaatacc ggaatcgaca gtcaataata ttttagcggc aatagttgaa
     2641 tcaaaatact tatcctatgg gacgtcctgc aatatcgata tttcattttt aagtcttaat
     2701 gttgctgttg tcacatatta cagtttcaaa ccacaggata tttcacttga tatctcaacc
     2761 ggtaacaatt ttagcttgac gatagatttt aaaataaaag ccaattttga cactccaatt
     2821 tttgattttc tattaaaagc ttctaatcta tcagtaacaa tcaatggaac aattgaccgg
     2881 gttgagcaag gatctggcta taaaattcga ttaacaccat taagccttta ctataacaac
     2941 aatgattggg tgaatattgt acttcacgac caactctcgg gactgatagc taaattaccg
     3001 gaaatcagca ccagttcaag ccaaccctta ttacccaata ttgtagcttc gtatttcacg
     3061 tcaggaacgc caactttaac gacaaccgat actagtatcg ttcttggatt gaccatggtt
     3121 gccgggctaa ggaatattaa agtacaaaat gaagttaatc aactcaacac gctcggaaat
     3181 gtgcaaacgg ttgtcaatgg atctgtcacc ggaacatatc catcaccaaa gacattcgaa
     3241 tggaataccg gtaacgtcaa gcaggtgcag acgccaaacg aactgattgc ctcgactaat
     3301 ggacagaata agtataagaa ttggtataag gatgatgcga cactgccaga gacggacatt
     3361 gctactagac gtattgaaat aacggttgct tcgagcgatg cgacttatac ggctaaattt
     3421 ctgcaagctc agcgtgtcca attatccaat gttctggagg gcagtctgac aggtggaact
     3481 gtgacttatg aaagtacaac aggttcaatg tatgatagtt atgaatactg ttataatgct
     3541 aatcctgttc cacattctat tagcacaaat gtacctagtg gtaccctcaa ctctgattgg
     3601 gtattttacc attggagtga cggtaatacc agtcaaacca gaaatattgt agtcgatagc
     3661 gataaaaacc tgcaggcgag ttggaagggc attcaccgct ccaatgccgc aaccgctttt
     3721 tccaacaaca gtcagcgtaa gtttgtccgt acccaggatg gctggctaca ccaagtctat
     3781 gaatcaatgg ggcgtgtctg gttagagcac agtagtaata atggctcaac atggtttctg
     3841 ggaaacaatg ggttgccttt agatgatgga gaaggcaaat gtccttcgat cgattggggc
     3901 cactattatt atagtagcag ttcacaggga acctacgtac atgaacatat tatcgttgtt
     3961 gcatatcagc agaaattcgg taactattac aaaattcggt atgccgtttt taaaaaagat
     4021 aataatgtgt atgtaaatta tactcccagt ggatcatatg caaccctgta tacagaatct
     4081 tcagaccagt attccgttga cgcaaacccg aatattgcaa taagtggatg ttcatccggt
     4141 atatatgatt ttattatcag ttttgaaaga aagagcggtt cgatggctgg tatcaactgg
     4201 ttctatggca agatggctta cattggtatt tatccgactt ataataatta tgttagccca
     4261 gtatggatta caggaaccaa tgccagttcg ataaatgcca gcgttcatct gaacaaggat
     4321 attgtgaatc cgagagagag ttttgacatc gtttaccagc aaggtactca atatataaag
     4381 gatgttatct tgagttgttc ctataatggt gttgggaatt ggacaacata ccagtttgga
     4441 ccatttacca tttcacaagg tacagacaga aaaagctata aaccctcatt ggtgcaaaaa
     4501 accaataggg atataagtgt tagctggatt cgcgatcgaa cggctgatgg cggttctccg
     4561 tattatatca atgccgtcaa ttgggattcg gaaaacagca gtgtttatag gatttacggt
     4621 tcttgggatg ttcgttcggt ctcttcaaat gtgcgtgatg ataacaccgc ggtttatacc
     4681 gcctggtcag aagctcaata tatcggcagt agtgcatcaa agaattgtgt ggccaggggt
     4741 tcaagttcga tcaccctaaa tactcatggc aagtatctgc aattatgtaa cggaccgctt
     4801 tcgggtggga gtacaaatat gcgggtatca gcctacgata tctccagttc tccttatttt
     4861 tttgaaacgt caaatgcaat cggtacatca ttacctaaat caactgtttc tgacatcaat
     4921 tatggaagag gtattgcact tgataatgga aatataggat ttttctactc gatcaaaaaa
     4981 ttgtcagctg gtaaacaagc aattcatttt attgaaattc ccacaaaacc gaatataatc
     5041 agatcagtgg aaaatgcgtc aataaaacct ttaatgcttg atagtttaaa tgcttatctg
     5101 ctaagtgaac ctttttcagt tacggaagac atggagataa cattcgttga ggatggtggt
     5161 tttactaagt tactcccatc cacaggtaat gttgatacat tgaaagcagt tgaaaaaggg
     5221 agacatatca aatgcaaatt agagctgatt gatgacgata cacagaaatt agtaggtact
     5281 ataagggtaa cacagttcaa tttag
//
LOCUS       DAOSMP010000087         4939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_17193_length_4939_cov_11.888411, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000087 DAOSMP010000000
VERSION     DAOSMP010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17193_length_4939_cov_11.888411"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PKX09_09490"
     CDS             complement(<1..236)
                     /locus_tag="PKX09_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18092.1"
                     /translation="MKTEEREQKERIQNHICRFQTGDFMTNAESLFTTLGYASDRRVK
                     IDKDYLCNKGLNQEKALFSDWREAGLLYQVTGEDL"
     gene            complement(248..3598)
                     /locus_tag="PKX09_09495"
     CDS             complement(248..3598)
                     /locus_tag="PKX09_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOU18093.1"
                     /translation="MVIDSSIRDNRNRGSVADFLLENLKANSKVNIVSAYFTIYAYET
                     LKNSLDGIEKLNFLFGEPKFVKALDPSKTDKKTFKIEDDGITLSNRMRQSQVAKECAE
                     WIQKKVDIRSIKQTNLMHGKLYHIENNGVEVGLMGSSNFTQRGLGVATSSNIELNMVV
                     DSDRDRKDLKNWFFEIWNDETLVEDVKKEVLQYLEQLYLDQPPEFLYFKTLYHIFKEF
                     LEDQTKGGLLAEQHLLTETEIWKILFDFQKDAVKGAINKINKHRGCIIADSVGLGKTF
                     EALAIIKYFELHNERVLVLCPKKLKDNWTVYQAHNASSLNPFLKDRFGYSILCHTDLS
                     RANGFSGDIDLANFQWGVFDLVVIDESHNFRNNIKGKRDEYGNVVRRSRYERLMQDIL
                     KSGVHTKVLMLSATPVNNTLSDLRNQISFITEDKDNAFAASESMGIPSIKETLRVAQM
                     KFTEWAAKRVKEQRTSRDLLNELDSAFFRLLDELTIARSRKHIHRYYQGEIDKIGGFP
                     KRSKPESIFSEIDLSGRFLSYDKVNDEIEKYALSLFNPSKYILSEWVSHYEEKARTSG
                     FTQFTQRDRESFLIGMMKINFMKRLESSIESFEITIGRTIEKIEDLEKKIDQFQQLKN
                     KPVEFDNSETEIPTDEDEELKDAFEVGKKVTFKLEHLDLDRWLTDLKKDKDQLSVLYN
                     SAVLVSPNKDAKLKDLKSLIEKKVNHPTKNSQGKVNKKVLIFTAFADTAKYLYNEIKN
                     WAENDLNVHIALVTGGSGGCTTTFAPKGFTGRTDYAWILANFSPISKKRNQMKGMPQE
                     GEIDILIATDCISEGQNLQDCDYLINYDIHWNPVRIIQRFGRIDRIGSLNPEVSLVNF
                     WPTKDLEKYIKLKYRVEARMALVDITATAEDNILSTDEIKDLIKDELKYRDKQLMRLK
                     DEILDLEDFDESVNLSEFTLDDFRIELANYIEANKKLLEAAPLGLYSVVPHVIERTTL
                     FDQKIAEIIQPGVIFCLMQKQNAIENQKVNPLQPYFLVYIRDDKVVRFTFTHPKQILE
                     IFRLLCVGQTKPFEDLCRQFDSETQNGNDMQKYTELMKTAVKSIVHTFEKRATGQLFD
                     SRGAVLPPQEAFLNEETEFELITWLVIK"
     gene            complement(3701..4132)
                     /locus_tag="PKX09_09500"
     CDS             complement(3701..4132)
                     /locus_tag="PKX09_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18094.1"
                     /translation="MSQSVDELLQDAGVRLATARKYAKELGTAGISAEYLTQFEGQIG
                     TLRTENTAYGEAKNRKSELSESQGVQVLASQDVIRKLRAAAQSAFYGDKVTQKEFGIG
                     KDVPATVKALSTELPLLKEVAGRYGFFTLSSSTCRLRLFKW"
BASE COUNT         1232 a         1141 c         1026 g         1540 t
ORIGIN      
        1 aggtcttcgc ctgtgacctg atagagcaaa ccggcttccc gccagtcgct aaataaagcc
       61 ttctcctgat ttaagccttt attgcagaga taatctttgt caatttttac gcgccggtcg
      121 ctggcatatc ccagggtcgt aaatagactt tcggcattgg tcataaaatc gccggtctga
      181 aatcgacata tatggttttg aattcgctct ttttgttccc gttcttccgt tttcaattta
      241 tttcccttta tttaattacc aaccaggtga tcaattcaaa ttcggtctct tcattaagga
      301 aagcctcctg tggtggtaaa acagctccac gcgaatcgaa aagttggccg gttgcgcgtt
      361 tctcaaaggt atgcacgatc gacttgaccg ccgttttcat cagctcagta tatttctgca
      421 tatcgttgcc gttttgcgtt tcggagtcga attggcggca caaatcctcg aatggtttag
      481 tttgacccac gcataataat cggaagattt ccaatatttg tttggggtga gtaaacgtaa
      541 aacgtacaac cttatcatcc cggatataga cgaggaaata aggttgcagc ggattgacct
      601 tttggttttc aatggcgttt tgcttttgca tcaggcaaaa gataacgcct ggctgaatga
      661 tctcagcgat tttctgatca aataatgtag ttctttcgat aacatgcggg accaccgaat
      721 ataagcctaa gggcgctgcc tccaacagtt tcttgttggc ttcgatgtaa tttgccagtt
      781 caatccgaaa atcatccaaa gtaaattcgc tgaggttgac cgattcgtca aaatcttcga
      841 ggtcgaggat ttcgtccttg agtctcatta actgtttatc gcgatatttc agttcgtctt
      901 tgatcaaatc cttgatttca tcggtgctca aaatattatc ttcggcggtt gccgtaatat
      961 caactaacgc catgcgggct tcgacacggt atttgagttt gatatacttc tcaaggtctt
     1021 tggtcggcca gaaattgacc aaagagactt caggattaag tgaaccgatc cggtcaattc
     1081 ttccgaatcg ttgaataatc cgcaccggat tccagtgaat atcgtaattg atcagataat
     1141 cgcaatcctg taagttttgt ccttcggaaa tacaatcggt cgcaatcagg atgtcaattt
     1201 cgccttcctg tggcatacct ttcatctggt tgcgtttctt ggaaatcggt gagaagttcg
     1261 ccagaatcca ggcataatcg gttctaccgg taaatccttt cggtgcgaat gtcgtcgtac
     1321 agccgccgga accaccggtc actaaagcaa tatgcacgtt gagatcattt tccgcccaat
     1381 tcttgatttc gttgtatagg tatttggcgg tgtcagcaaa agcggtaaat atgagtactt
     1441 tcttgttcac ttttccctga ctgtttttcg tcggatggtt gactttcttc tcaattaggg
     1501 atttaagatc ttttagtttg gcatctttgt tcggtgatac gaggacggca gagttgtaga
     1561 gaacgctcaa ctgatcttta tcttttttca ggtcggtcag ccatctgtcc aaatctaaat
     1621 gttcgagttt gaacgtgact tttttaccaa cttcaaaagc atctttcaat tcttcatctt
     1681 catcggttgg tatttccgtt tcggagttat caaattccac cggcttattt tttaattgct
     1741 gaaattggtc gattttcttt tccaggtctt cgatcttttc gatggtacga ccaatggtga
     1801 tttcgaacga ttctatcgaa ctctctaacc gtttcatgaa attgattttc atcatcccga
     1861 ttaaaaaact ttcacgatcg cgctgggtga attgggtaaa tccggacgtt cgggcttttt
     1921 cctcataatg agacacccat tcggacaaaa tgtatttcga tggattgaat agagacaggg
     1981 catatttctc tatttcatca ttgaccttgt cgtatgataa aaacctgcct gataagtcta
     2041 tctcagaaaa gatggattcc ggtttagagc gttttgggaa accaccgatt ttgtcgatct
     2101 ctccctggta gtaacgatgg atatgtttac gggagcgagc aatcgtaagt tcatccaata
     2161 atcggaaaaa cgcggaatct agttcattta gcaaatcgcg ggaggttctt tgttccttta
     2221 cacgttttgc cgcccattcc gtgaatttca tctgtgcgac tcttagagtc tccttgatgc
     2281 tgggaatccc catagactcc gacgcggcaa aggcattatc tttatcttcg gtaatgaatg
     2341 aaatctgatt ccggagatcg ctcagcgtgt tgttgacggg cgttgcggaa agcatcagca
     2401 cttttgtatg gacaccggat ttaagaatat cctgcatcaa tctttcatag cgactacgcc
     2461 gaacgacatt cccgtattcg tctcgtttgc ctttgatgtt gtttctgaaa ttatgcgatt
     2521 cgtcaatgac gacgagatca aaaacacccc attggaaatt cgccagatcg atgtcgccgg
     2581 aaaatccgtt cgctctgctt aaatcggtat ggcagagaat ggaatagcca aaacggtctt
     2641 tgagaaacgg attaagagaa ctcgcattgt gcgcctgata aaccgtccaa ttatccttta
     2701 gtttcttcgg acaaagcacc agtacccgct cattatgcaa ctcaaaatac ttgataattg
     2761 ccagcgcttc gaacgttttt ccgagtccaa cgctatctgc gataatacaa ccgcggtgct
     2821 tattgatctt gttaattgcg cctttgacgg cgtctttctg aaaatcgaag agaatcttcc
     2881 agatttcggt ctcggttagg agatgctgtt cggccagcaa tccgccttta gtttggtctt
     2941 caaggaattc cttgaatata tggtagagcg ttttaaagta gagaaattcg ggcggttggt
     3001 ctaagtaaag ttgttcaagg tactgtagta cttctttttt aacatcttcg actagagttt
     3061 catcattcca aatctcaaaa aaccagtttt ttaaatcctt ccggtctctg tcactatcga
     3121 cgaccatatt taactcgatg tttgaggaag tggcaacacc taaccctctt tgggtgaaat
     3181 ttgaacttcc catcagcccc acttccacac cgttattttc gatgtggtac agtttaccgt
     3241 gcatcagatt ggtctgttta atcgagcgga tgtcaacttt tttctgaatc cattcagcgc
     3301 actctttagc aacctgactt tgacgcatcc ggttcgaaag agtgattcca tcatcttcaa
     3361 ttttgaatgt ctttttatcg gttttactcg gatccagtgc cttgacgaat tttggttcgc
     3421 cgaatagaaa gttcagtttt tcgattccat cgagagaatt tttcaatgtc tcataggcat
     3481 agatcgtaaa atatgccgag acgatattca ccttagaatt cgcttttaaa ttttccagaa
     3541 gaaaatccgc tacgctacca cgattgcgat tgtcacgaat ggaagagtct ataaccattc
     3601 aaaatcccct taggtttttt atgatttttc aaagattgcc gagaatgatc acagtacgca
     3661 cttattgtaa cttattttcc aacattaaac aagcagattt tcaccacttg aacaggcgca
     3721 ggcggcaggt agaagacgaa agagtaaaaa aaccataccg cccggcgacc tccttaagca
     3781 ggggcagctc ggtcgaaagg gctttgaccg tagccgggac gtccttgccg attccgaact
     3841 ctttctgcgt gaccttgtcg ccgtagaaag ccgactgggc cgccgcccgc aacttacgga
     3901 tgacgtcctg cgaagccagg acctggacac cctggctttc ggacaattcc gatttccggt
     3961 tcttggcctc gccataggcc gtattttccg ttcggagggt tccgatttgt ccttcaaact
     4021 gggtcagata ttccgcactg attccggcgg ttcccaattc cttcgcatac ttccgggccg
     4081 tggccaatct gaccccggcg tcctgcaata attcatccac gctttgagac ataagtcctc
     4141 ctttgtttta ccctcccgcc ggttatttcc ggccgggaga atgtttcaac ttcttagaat
     4201 atttttttgt cttgttcatt tgtcaacttg catccgttga tagtttttta ccaccttaaa
     4261 gcgtttgatt tccacccgta agcaaaataa atctatccgc agacaattta ttaccacccg
     4321 cgactaattt ggaaccatcc cagagcaatt tataaccacc ctttagccgt ttggaaccgc
     4381 ctgcgggcaa aatatttaca cctttttgtt attgggaaac atctgtgagt aatttggcgc
     4441 aaccctacac taatttattt ccgccctttt gtcgttgggc gcaaactttt tgcaatttgg
     4501 aaacgccctg gagtcgtttg taaccaccta agaacaattt atcccggcct taccccaatt
     4561 tatttccgcc cgcggccaaa tattttccgg atgaatgcaa aacagagccg cccgggcgaa
     4621 aaaccctgaa gccgttgacc ggattataaa cgctccgcta aaattgtccg ccggaaaaaa
     4681 cggttgcgaa aagaccctcg cttgttaaaa agcaaaagac tcaaaatacg caccggactt
     4741 tcatatttat aatgttacgt tatcccgacc ggccttaatt tattcacagg cgcggggtat
     4801 gcaataaaat atttgtcggt ttcggccgca tttgaatttt ccgggtcgtt tatgggcgcc
     4861 gcttgaaaat tccccccgtt tcctgtttgc aattaacctc cagttgttct atatttgccg
     4921 gttaattatg atagacatc
//
LOCUS       DAOSMP010000088         4668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_18413_length_4668_cov_18.890960, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000088 DAOSMP010000000
VERSION     DAOSMP010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18413_length_4668_cov_18.890960"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1432)
                     /locus_tag="PKX09_09505"
     CDS             complement(<1..1432)
                     /locus_tag="PKX09_09505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOU18095.1"
                     /translation="MPASYLTEPRDFHSISLWQQVTEKQWHDPIWQRKNAIREVDELC
                     QVIRLNDYQKGEIAHTIAKLRAEGKEPMRITPYYASLIAEDPFHPNLLPSEEAENRLD
                     PIFWEAVPTPAHLLFPHTGSEGAMAEENRSYGSAYQRYPNRVALFVAENTSCASYCTH
                     CQRLKSLDCTTTISRQDIDKGLFYIDWNPNIDEVLVTGGDALMISPERLEYVLTRLSK
                     IDHLRVIRIATRVPVTLPMAITEDILSLIDRATSNSGKYVYFMTHLNHYHEITPEFKS
                     AIDRIHRHGYSIRNQTVLLKHVNDNLGSLAETSRRMFWAGVEPYYLLQCHRERGLVHF
                     ITPIQIGKIFVKHLQGWISGMARPIYAANIEGGGGKIILMPSGHDTMQKGFNLEEYIS
                     ESYATIYTWDNKIIDDYEALGRATQAEYDAAVKTMDKFIGRKGAFRPSLIIVDKNGNH
                     ITTTNKSPLPSFSNRDKVKIFNYWVDS"
     gene            1798..2787
                     /locus_tag="PKX09_09510"
     CDS             1798..2787
                     /locus_tag="PKX09_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU18096.1"
                     /translation="MEQLNDVELLTALTTAHKRLNKEIGRVIVGQQEIINQIVISLLS
                     RGHCLLIGVPGLAKTLMIKTLAQALDLQFRRIQFTPDLMPSDITGTEVIEEDKLTGAR
                     AFRFFKGPIFGNIILADEINRTPPKTQSALLEAMQEHRVTAAGETHQLEEPFFVLATQ
                     NPIEQEGTYPLPEAQLDRFMFSLKVEYPTFQEELEIVKSTTVDADSTPEIVIHRAEIL
                     QFQHLVRRVPVAPNVIEYAVKLVTATRPTNSSAPDFIQKWVEWGAGPRASQYLILGAK
                     ANAVLEGRPTPSIQDVIKMAKPVLRHRIIPNFNAEAEGINTDHIIQKLLETIG"
     gene            2808..3713
                     /locus_tag="PKX09_09515"
     CDS             2808..3713
                     /locus_tag="PKX09_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOU18097.1"
                     /translation="MAEERLDKRRYLEPAVIARLDNMALRARLVVEGFIIGLHRSPYH
                     GFSIEFAEHRPYMPGDEIKHIDWKLYGKTDRFYIKEFEEETNLKSYIILDKSGSMGYQ
                     STAVSKLAYGSYLAAALGYLLLKQQDAVSLTLFDTEVTTYLPPVAKKSHLNLVLGALE
                     KVTAGSETSIAPLLHSAAEKIKKRGLIILISDLLDDPSSVINALKHFRHRRHEVIVFQ
                     VLDPQEIEFGFNRQTKFVDLENRDVLLTEPGQVRLAYRQAINNFIEDYKIQCRANNID
                     HVLLTTDRPLDLALTEYLNKRARIG"
     gene            complement(3769..>4668)
                     /locus_tag="PKX09_09520"
     CDS             complement(3769..>4668)
                     /locus_tag="PKX09_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU18098.1"
                     /translation="IQKYPDNAQAYLLLGSAIYRPTKRYDEAKQMFEKAKQIDPTKAK
                     EADDNIRGMWAELHTEGANLFNSALKAFLPAEKDSLLNLAAQQFIRALEFKSDEIPTY
                     NGLVKCFYILNDSANVEKYGKMMLDKNLFDEDVVNYYFQVAWDSTRQEEIFAQLDKII
                     AEHPDNIELKILKIQFLSDVGRDEEALDIINKLIENDPANLDLVYIAAQLLTKLGRFD
                     EAKYQFQKVIANDPTNLELLIRVTEAVFRDKDWVEAEYYARKIIELDSNNVFGYEVLW
                     KSLYNQGNIEEAEKYRMIQKSLE"
BASE COUNT         1315 a          982 c         1006 g         1365 t
ORIGIN      
        1 cagaatctac ccaataatta aaaattttaa ccttatcgcg attgctgaaa cttggtaaag
       61 gactcttatt agttgtcgta atgtggttgc catttttatc aacaataatc aaagatggtc
      121 ggaaagctcc tttccgcccg atgaatttat ccattgtttt gaccgccgca tcatattcag
      181 cctgagtagc gcgtccgagt gcttcgtaat cgtcaatgat cttattatcc caggtataga
      241 tcgttgcata gctttctgag atatactcct cgaggttaaa acctttttgc atggtatcgt
      301 gtccagaagg catcagaatt atttttcctc ctccaccctc gatattggcg gcgtaaatcg
      361 gccgagccat tccgcttatc caaccctgaa gatgtttcac aaaaattttg ccaatttgaa
      421 ttggagtaat aaaatgcact aaacctcgtt ctcgatgaca ttgaagtaga tagtaaggtt
      481 ctactccagc ccagaacatt cgtcgcgagg tttcggcgag tgacccgagg ttatcattga
      541 catgtttcaa caaaacagtt tgattgcgaa tcgaatagcc atgccggtga atgcggtcaa
      601 tcgccgattt gaattccgga gtaatttcat gataatgatt aaggtgagtc ataaagtaaa
      661 cgtattttcc ggagttagaa gttgctcgat ctataagaga taaaatatcc tcagtaatag
      721 ccattggcaa agttaccgga acgcgagtag cgatccttat aacccgtaaa tgatcaattt
      781 ttgataaacg ggttagaaca tattcgaggc gttcgggact aatcatcaaa gcatcgccac
      841 cggtcactaa aacttcatca atattaggat tccaatcaat ataaaatagt cccttgtcaa
      901 tatcttgccg actgatagta gtagtacagt cgagtgattt tagtctctga caatgggtgc
      961 agtaggaagc gcagctggta ttttcggcaa cgaagagcgc aactctatta ggatagcgtt
     1021 gataggcaga cccataactg cgattttctt cagccatcgc gccttcactt ccggtgtgag
     1081 gaaataacag atgagccgga gtaggaacgg cttcccagaa aataggatcc agccgatttt
     1141 cggcttcttc gctaggtaat agattggggt ggaaaggatc ttcggcaatt agggacgcat
     1201 aataaggtgt aatgcgcatc ggttctttgc cttccgcacg taatttggcg atagtatggg
     1261 caatttcacc tttttgataa tcgtttaacc gaatcacctg acatagttcg tcaacttcac
     1321 ggatggcatt tttcctctgc cagatgggat catgccattg cttttcagta acctgttgcc
     1381 agagtgaaat tgaatgaaaa tcgcgtggct cggttaaata agatgcgggc atcgttaatt
     1441 tctcctaagt tttatgtagc ttaaattatt ataaaatgtc attattcttt tggaatctaa
     1501 aaaacagtcg ttatttatgc aaatatacca tttgctaata aaaatcgaga gcagtaccga
     1561 tgaagtaggg tattctcgag gctaaaataa gtagccggtt agaaattatt atgccggggt
     1621 catctacgtt tatataaatt tatattgggt taaaattcta atgtaccggg ctgacactca
     1681 gggagaattt atcaaattgg ttatttatca tctggcaggc tccgacaatt attggcaaat
     1741 tgagcggaaa gtcttagatt ctcaacgctt tcaactttat aatcggaatg aaaatatatg
     1801 gaacagctta acgatgtcga attgctgacg gctttaacta cagctcataa gcggcttaat
     1861 aaagaaattg ggcgagtgat agtcggccag caggaaatta ttaaccaaat tgtgatttct
     1921 ctcctttcgc ggggacattg tcttttgatt ggcgtgccgg gattagccaa gacgctaatg
     1981 attaaaacct tagcgcaggc gcttgatctg caattcaggc gtattcaatt tacgcctgat
     2041 ttaatgccgt ccgacattac cggcaccgaa gttatcgaag aagataaact gaccggcgct
     2101 cgcgcttttc gatttttcaa agggccgatt tttggcaata ttattctcgc tgacgaaatc
     2161 aaccgcactc cacctaaaac ccaatctgct ttgctggaag cgatgcagga acatcgcgtt
     2221 accgccgccg gtgaaacgca ccaattggag gagccgtttt ttgtattagc gacccagaat
     2281 ccgattgagc aagagggcac ctacccgttg ccggaagcgc agcttgatcg ttttatgttt
     2341 agtttaaaag ttgaataccc gacctttcaa gaagaactgg agattgtcaa gtcaaccacc
     2401 gttgatgccg actccacacc ggaaattgtc attcatcgag ccgaaattct gcaatttcag
     2461 catcttgtta gacgagtacc ggtggctccg aacgttattg aatatgcggt gaagctggta
     2521 actgctacgc gacctactaa ttccagcgcg cctgatttta ttcagaaatg ggtagaatgg
     2581 ggagcaggac cgcgtgccag tcagtatttg attctcggcg ctaaagcgaa tgccgttctg
     2641 gaaggtcgtc caactccgtc aattcaggac gtaattaaga tggcgaagcc ggtgctgagg
     2701 catcgtataa ttccgaactt taatgccgaa gccgagggaa ttaataccga tcacattatt
     2761 caaaagttac tcgagactat cgggtaatct ggggaatgtt tttataaatg gcagaagaaa
     2821 gactggataa acggcgttat cttgaaccgg cagtcattgc tcggttggat aatatggctt
     2881 tgcgcgccag actggtagtg gaaggcttta taatcggcct ccatcggagt ccttatcatg
     2941 gcttttcgat cgaatttgcc gaacaccgac cctatatgcc gggcgatgaa attaaacata
     3001 tagactggaa gctttacggt aaaaccgatc gcttttatat aaaagaattc gaagaagaaa
     3061 ccaatcttaa gtcctatatt attctcgata aaagcggttc gatgggctac caatcaaccg
     3121 ccgtttccaa gttggcttac ggtagctatt tagccgccgc gctgggatat ttattactta
     3181 aacaacaaga tgcggtcagc ctgactcttt ttgataccga agttactacc tacttaccac
     3241 ccgtcgctaa aaagagtcac ttgaatttag tactcggagc tttggagaag gtcaccgccg
     3301 gttcggaaac ttccatagcc ccgctcctgc attccgccgc tgaaaagatt aagaaacgcg
     3361 ggctgatcat tcttatttca gatttgttgg acgatccttc aagcgtaatc aatgccctga
     3421 agcattttcg ccatcgccgt cacgaggtaa tcgtgtttca agtgcttgac cctcaagaaa
     3481 tcgagtttgg tttcaatcgc caaactaaat tcgttgatct ggaaaatcgt gacgtgttat
     3541 tgactgaacc cggtcaggtg agattagcat atcggcaggc gatcaataat tttattgagg
     3601 attataaaat ccaatgccgg gcgaataata ttgatcatgt cttattaact accgaccgcc
     3661 ctctggattt agccttgacc gaatatttaa ataaacgcgc ccgaattggt tagaaaacaa
     3721 aaaagccgga tcttcagtcc ggctttttca aaaaataaat cagcaggctt attccagcga
     3781 tttttgtatc atacgatact tctcagcctc ttcgatatta ccttgattat ataacgattt
     3841 ccagagaact tcatacccga atacattatt tgagtctagt tcgataattt tacgagcgta
     3901 atattccgct tccacccagt ccttatctct aaaaaccgcc tcggttacgc ggattagtaa
     3961 ttccaggtta gtgggatcgt ttgcgataac cttttgaaat tgatatttag cttcatcgaa
     4021 ccggccgagc ttagtcagta attgagcggc gatgtaaacc aaatcgagat tggcgggatc
     4081 attttcgatt aatttattaa ttatatcaag ggcttcttca tcgcgtccca cgtcggatag
     4141 gaactggatt ttaaggattt ttaattcaat attgtccggg tgttcagcga taattttatc
     4201 taactgagcg aaaatttcct cttgacgggt gctatcccag gcaacttgaa aataataatt
     4261 gactacatcc tcatcgaaaa gatttttatc cagcatcatt ttgccatatt tctcaacatt
     4321 agcagagtca ttcagaatat aaaaacattt taccaagcca ttataagttg gaatttcatc
     4381 ggacttgaat tccaatgcac gaatgaactg ctgagcggca agattcaaga gcgagtcttt
     4441 ttcagccggt agaaaagctt ttaaagcgct gttgaacaag ttggcgcctt cggtgtgtaa
     4501 ctcagcccac atccctctaa tattgtcatc agcctctttt gccttggtag gatcgatttg
     4561 cttggctttt tcgaacatct gtttggcttc atcatagcgt ttagtcgggc gataaattgc
     4621 gctacccaat aagagatagg cctgggcatt atccggatat ttctggat
//
LOCUS       DAOSMP010000089         4646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_18533_length_4646_cov_14.151383, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000089 DAOSMP010000000
VERSION     DAOSMP010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18533_length_4646_cov_14.151383"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..615
                     /locus_tag="PKX09_09525"
     CDS             250..615
                     /locus_tag="PKX09_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU18099.1"
                     /translation="MAKVLVVDDDPDFVSAVTIVLESAGHKVSKAYSRQEGMQVFAKE
                     KPDIVVLDVMMQEPDDGFVMAQDLKAKARVPILMLTNVGQVTGLDFDKDSEMVPVDAF
                     ESKPIKPSVLLERINQLLQ"
     gene            630..>1152
                     /gene="nuoE"
                     /locus_tag="PKX09_09530"
     CDS             630..>1152
                     /gene="nuoE"
                     /locus_tag="PKX09_09530"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOU18100.1"
                     /translation="MQVLDRVKINQEISDLKKTYGDDRSALLPILQELQEKYGYLPDL
                     VMQETAHALGIHPVEVESVASFYSFFHRKEKQGKYVLRLCQTISCDLSGKAKVARQLE
                     NELGIKFGETTKDGMFTLEYTNCLGMCDQGPALLINDRLFARVTPEKVPQIIADCRRD
                     FIKSAFPKVVPSDV"
     assembly_gap    1153..1252
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1253..2067
                     /locus_tag="PKX09_09535"
     CDS             <1253..2067
                     /locus_tag="PKX09_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOU18101.1"
                     /translation="LEKRRQNNLLGENILGKEGFNFDIEIRMGSGAYVCGEETALIES
                     LEGYRGEPRNRPPFPVDTGFQNHPTIVNNVETFLDAALICVKGAKWFNEHGTEKSSGT
                     KLFSVSGDCEKPGIYELPFGITIEQLLKEVKGENAKAVQIGGASGRCVARKDFNRTIA
                     FEDISSGGSIIVLGPQVDMLEVAINFMEFFVEESCGQCTPCREGNVKLLEGLKLLEEG
                     RCSASYLNELMRLAETIQLASKCGLGQSSPNAFVSIIENFKSEILGRVPEKA"
     gene            2079..4010
                     /locus_tag="PKX09_09540"
     CDS             2079..4010
                     /locus_tag="PKX09_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOU18102.1"
                     /translation="MEDNEKVMTESTVRAPKSTTPSTIKAPEDISAIGATIKVTIDGK
                     EIRVPMGTTILNAARMLGINIPTLCHHNDLCISGVCRICVVEVEGMRTLQASCAYPIS
                     QPIVVHTHTRKVRQARRHVVDLLLARHYGECYSCVRNNNCELQQLAHEYGVDFYRFGH
                     VDKPTYGRDNSSYAIVRDMDKCILCRRCVRTCIDLQEVGVYEAFERGNQTRIGTFMDN
                     PMAELICINCGQCINRCPTGALHANDPSDAIWAAIDDPTKHVVIQTAPAPRSAIGECF
                     GLPAGTPLTFEMNTAMRYCGFDKVFDTNFSADLTIIEEGTELILRLYKALVKKEPVSL
                     PLFTSCSPGWVKYIEHFYPEMLSHLSSAKSPQQMFGAVIKTYYAELNNIDPADIVTVA
                     LMPCTAKKFECNRPEMNSSGFKDIDYGLTTRELAGMFQEAGIDLPNMPKSDFDEPFGT
                     RTGSGVIFGATGGVMESAIRTVYELITGDRIDNLFERARIMPVRGFEGIRYAELPIAK
                     VGPVPDLLKHLIPNWDWLRGATLKVAVAHGTANAKKVLDNIKSGGKLSECHFIEFMAC
                     PGGCLGGGGMPIPTNEEIRAARAQAIYAEDEASEVRKSYENPAVVRLYNEFLVDGPCG
                     HKSHKLLHTYYTPRGKKLV"
     gene            4138..>4646
                     /locus_tag="PKX09_09545"
     CDS             4138..>4646
                     /locus_tag="PKX09_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOU18103.1"
                     /translation="MPNRFIYLDHNATTPLHPEVKNIITDMFDVFGNPSSLHTVGRIA
                     RQKIEQAREIVANFIGAAPDEIIFVGSGSEANNSVLNSLSCPSQVCRLERNLRDEIIT
                     TSIEHPCVLETSKCLRERGTTVHYLPVDITGKVKLDVLKELLSEKTALVSVMYANNEI
                     GTIQDIKAIA"
BASE COUNT         1267 a         1002 c         1029 g         1248 t
ORIGIN      
        1 cacgtgaaaa attaaataat tttacctaac tttgggcgct tttttggcaa gatagaaaat
       61 tagaaaaacg atacagaata gctaatatta tcaaatatcg agagtcgtaa aaaccgtatg
      121 aaaatctaca gccgaaccgc tccaatcctg agggagattt gtattacaac ctaattcatc
      181 cattagtttg tccattttgc ggatattatc tgatcaactt gatttattat ataaacggag
      241 gaattattta tggcaaaagt tttggtcgtt gatgacgatc cggatttcgt atcggctgta
      301 acaattgtcc tggaaagtgc cggacataaa gtatctaaag cttattcccg ccaagaagga
      361 atgcaagtat tcgctaaaga aaagccggat atagtcgttc tggatgttat gatgcaggaa
      421 ccggatgacg gttttgtaat ggcgcaggat ttgaaagcca aagcccgtgt tccgattttg
      481 atgctgacca acgttggaca ggttaccggt ctggacttcg ataaagattc cgaaatggtg
      541 ccggtagatg catttgaatc aaaaccgata aaaccatctg ttctgttaga aagaattaat
      601 caattattgc aataaaaaag gaaaaaccaa tgcaagtatt ggatcgcgtt aaaatcaatc
      661 aggagatttc cgacctgaaa aaaacctatg gcgatgaccg ctctgccctg ctaccgattc
      721 tacaggaatt acaggaaaaa tacggttatt tgccggattt agtaatgcaa gaaacggcgc
      781 atgctttggg cattcatcct gtcgaagttg aaagcgttgc cagcttttat agttttttcc
      841 atcgcaaaga aaaacaggga aaatacgttc tacgcctctg ccagacgatt tcctgcgatt
      901 taagtggaaa agccaaagtt gcgcgccaac tggagaatga actcggtatc aaattcggtg
      961 aaaccaccaa agatgggatg tttactcttg aatatactaa ctgcctcgga atgtgtgatc
     1021 agggaccggc tttgctaatc aacgatcgtt tatttgccag agtaacacct gaaaaagtgc
     1081 cacaaatcat tgccgactgt cggcgtgatt ttatcaaatc cgcctttccg aaagtagtac
     1141 catccgatgt cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntactcgag
     1261 aaacgccgtc agaataatct actcggtgaa aacatcctcg gcaaggaagg tttcaatttc
     1321 gacatcgaga ttagaatggg ttccggcgct tatgtctgtg gcgaagaaac cgccctgatc
     1381 gaatcgctgg aaggctaccg cggtgaaccg cgaaaccgcc cgccttttcc ggttgatacc
     1441 gggtttcaaa atcatccaac tattgttaat aacgtcgaga cttttctgga tgcggctctg
     1501 atctgtgtca aaggcgctaa gtggttcaat gaacatggca ccgaaaaatc cagtggcacc
     1561 aaacttttca gtgtttccgg cgactgcgaa aaacccggca tttacgaatt accatttggt
     1621 attaccatcg aacaattgtt aaaagaagtc aaaggcgaaa atgcaaaggc ggtgcaaatt
     1681 ggcggcgctt ccggtcgttg tgtagcccgt aaggatttca accggactat tgcctttgag
     1741 gatatttcgt ccggcggttc gattattgtt ttgggaccgc aggtggatat gctcgaggtc
     1801 gccatcaatt ttatggaatt cttcgtagaa gaatcctgtg gccaatgtac tccctgccgt
     1861 gaaggcaatg tcaaactgct cgagggttta aaactgctcg aagagggacg ctgttcagct
     1921 tcctatttaa atgaactgat gcgtctggcg gaaacaattc aactagcttc caaatgcggt
     1981 ttgggtcaat ccagtccgaa tgcctttgtc tcaattattg aaaattttaa atcagaaatt
     2041 ttaggccgtg tgcctgaaaa agcttaaaga ggatatttat ggaagataac gaaaaagtaa
     2101 tgactgagtc cacggttcgc gcacccaaaa gtactacgcc atccaccatt aaagctcccg
     2161 aagatatcag cgcgattggt gcgactatca aggtgacaat tgatggaaaa gaaattcgcg
     2221 ttccgatggg caccacgatt ttgaacgccg cccgcatgct cggcatcaat attccgactc
     2281 tctgtcatca taacgacttg tgtatcagcg gtgtttgccg tatttgcgtg gtggaagtcg
     2341 aaggtatgcg cactcttcag gcgtcctgtg cttatccaat cagccaaccg attgtagtcc
     2401 atactcacac ccgcaaagtt cgccaggcac gacgtcatgt tgttgacctt ttgcttgcgc
     2461 gtcattatgg cgaatgttat tcctgcgtcc gcaacaataa ctgcgaatta caacaattag
     2521 cccatgaata tggtgttgat ttctatcgtt ttggccatgt tgataaaccg acttatggtc
     2581 gcgataactc cagctatgca attgtgcgtg atatggataa atgtattctc tgtcggcgtt
     2641 gcgtccgcac ctgtattgac cttcaggaag ttggagttta cgaagccttt gaacgtggta
     2701 accaaacccg cattggcacc tttatggaca atcctatggc ggaactgatt tgcatcaatt
     2761 gcggacaatg tatcaaccgt tgtccgaccg gtgctttgca tgccaatgac ccaagtgatg
     2821 cgatctgggc tgctattgat gatccgacta aacatgtcgt gattcagacc gctccggcgc
     2881 cgcgttccgc cattggcgag tgttttggtt taccggctgg cactcccctg acttttgaaa
     2941 tgaataccgc tatgcgttac tgcggtttcg ataaagtttt cgatactaac ttttccgccg
     3001 atttaaccat tattgaagaa gggaccgaac taattttgcg gctttacaag gctttagtaa
     3061 agaaagaacc cgtgtctctg ccgctgttta cttcctgttc gcctggatgg gtaaaatata
     3121 tcgaacattt ttacccggaa atgttatcgc acctgtcatc agccaagagt ccccaacaga
     3181 tgttcggcgc cgtcatcaaa acctattatg ctgagcttaa taacatcgat ccggccgata
     3241 ttgttaccgt tgcgctgatg ccctgtactg ccaagaaatt cgaatgcaat cgtcctgaga
     3301 tgaattcatc cggttttaaa gatattgatt acggcttgac gacgcgcgaa ttggccggga
     3361 tgttccagga agccggaatt gatttaccta atatgcctaa atcagatttc gacgaaccgt
     3421 tcggaactcg caccggctcg ggcgtcattt tcggtgcaac cggtggcgtt atggaatcag
     3481 ctattcggac tgtctatgaa ttaatcaccg gtgatcgtat tgataaccta ttcgaacggg
     3541 ctcgcataat gcccgtgcgc ggtttcgaag gcatccgtta cgctgagctt cccattgcta
     3601 aagtcggtcc ggtgccggac ttactaaagc atttgattcc taattgggat tggctgagag
     3661 gtgcaactct gaaagtagcg gtagcacatg gaactgctaa cgccaagaag gtgcttgata
     3721 atattaaatc cggcggcaaa ttaagtgaat gccacttcat cgaatttatg gcttgcccgg
     3781 gtggttgtct gggtggcggt ggaatgccga ttccgaccaa tgaagaaatt cgagccgctc
     3841 gagcgcaggc tatctatgct gaggatgaag ccagcgaggt tcgcaaatcc tatgaaaatc
     3901 cagccgtggt caggctatac aatgaatttc tcgttgatgg accctgtggt cataaaagtc
     3961 ataaactctt acatacttac tatacgccac gcggtaaaaa attagtgtaa caatcatcct
     4021 cctctttatc tctaaaaagg gcaatctttt agtagattgc ccttttttaa taaatgaggt
     4081 cgtagtaatt ttgaaactga gtaattgaaa aaaagaattc atttcattga ggataaaatg
     4141 ccgaataggt ttatttattt agatcacaat gctaccactc cattacaccc ggaagttaaa
     4201 aatattatta ccgatatgtt cgatgtattt ggcaacccct ccagtctcca caccgtcggt
     4261 cggatcgctc gccaaaaaat cgaacaagcc cgtgaaattg tcgcaaattt catcggcgcc
     4321 gcgcccgatg aaattatctt cgtcggaagc ggtagtgaag ccaacaattc tgtccttaat
     4381 agtctctcct gcccttcgca ggtttgtcgc ctcgaacgca acctgcgcga tgaaatcatc
     4441 acaacctcta tcgaacaccc ttgcgttctg gaaacttcca aatgtttacg agaacgcggt
     4501 acgactgttc attatttgcc agtggatatt accggaaaag ttaaactcga tgtacttaag
     4561 gaattactgt cagaaaaaac cgcattggtt tcggttatgt acgccaataa tgaaatcggt
     4621 actattcaag atataaaagc tattgc
//
LOCUS       DAOSMP010000090         4636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_18588_length_4636_cov_20.933202, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000090 DAOSMP010000000
VERSION     DAOSMP010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18588_length_4636_cov_20.933202"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PKX09_09550"
     CDS             <1..534
                     /locus_tag="PKX09_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU18104.1"
                     /translation="TESEIENLGFIIQKRLPESGDWLLVADYTTCEALAGHGSTSEAH
                     EYSYTDAAVIPGATYLYRLGDVDYKGKVTWHKEVEVKVEAENAQVPLVFGLKPAYPNP
                     FNPSVTIPYGLAEDGQMSLKVYNLRGELVEVLISTYALKGTYSYNWSPQNLSAGIYLV
                     RLQSGNHTSMQKIVFVK"
     gene            697..1722
                     /gene="hypE"
                     /locus_tag="PKX09_09555"
     CDS             697..1722
                     /gene="hypE"
                     /locus_tag="PKX09_09555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOU18105.1"
                     /translation="MASDFSDKQKITSAHGAGGKYSHDLIKQVFLKHFDNPPLRELLD
                     SAILPERHGRLVFTTDSHVVQPLFFPGGDIGKLAVSGTVNDLAVCGAKPLWLSCGMII
                     EEGLEIETLEKIVISMQKTAQSAGVQIVTGDTKVVGHGEADGLFINTAGIGYLPENRN
                     IAKSLITPGDKIILNGFIGDHAAAIIKARHQFQIDLDIISDCAPLNNLTEVLYENITG
                     IRIMRDPTRGGLATTLNEFLDGNRFGMRISEEFVPIRNVVQGLCEPLGFDPFYLANEG
                     KVVVVVAEKSAEKALQTMRSHPLGRNAALIGEVIAQPAGHVLLKTRIGTERILDMLTG
                     EMLPRIC"
     gene            1899..2276
                     /locus_tag="PKX09_09560"
     CDS             1899..2276
                     /locus_tag="PKX09_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOU18106.1"
                     /translation="MDENDISRIIVDCAYHIHRELGPGLLESVYEKVLMYELEKKGLN
                     IDNQVAIPVRYHEISLELGFRADLIIENKIIIELKSVEEILPVHKKQLLTYLKLTGLK
                     LGLLINFNSNLIKDGITRIVNNL"
     gene            2450..2827
                     /locus_tag="PKX09_09565"
     CDS             2450..2827
                     /locus_tag="PKX09_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOU18107.1"
                     /translation="MDENDISRIIVDCAYHIHRELGPGLLESVYEKVLMYELEKKGLN
                     IDNQVAIPVRYHEISLELGFRADLIIENKIIIELKSVEEILPVHKKQLLTYLKLTGLK
                     LGLLINFNSNLIKDGITRIVNNL"
     gene            3017..3370
                     /locus_tag="PKX09_09570"
     CDS             3017..3370
                     /locus_tag="PKX09_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOU18108.1"
                     /translation="MHELKIAQNIIAIAADELARRQISTAVRVIHFKAGQMHAIIPET
                     LMFNFDTIKSEIPQFAHAVLSIEIIPLRIKCHQCQNISELDEPVFECRHCHGTDLEII
                     NGQEMFVDNIEMEDG"
     gene            3486..4142
                     /gene="hypB"
                     /locus_tag="PKX09_09575"
     CDS             3486..4142
                     /gene="hypB"
                     /locus_tag="PKX09_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOU18109.1"
                     /translation="MEIKIVRHVLEANDKLAAEIRQRTANSKTLLVNLMSSPGSGKTT
                     LLEKLIPMLHAKAYKIGVIEGDITTTMDAERLQPLNIPIVQINTEQFGGDCHLGAEVI
                     LPALDSLDLAKLDFVFIENVGNLVCPAEFDTGADVNVVVLSVTEGEDKPLKYPLMFRV
                     CHLALISKTDLLPYLNLNLDLLHQNMVKINPQLTIFPISAQRGDGLADLRDWLIEHKR
                     "
     gene            4214..4588
                     /locus_tag="PKX09_09580"
     CDS             4214..4588
                     /locus_tag="PKX09_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU18110.1"
                     /translation="MLKKILVIDDDVDLIEVLRLMLEKNGYLVIDAQNGTRGYQLVEK
                     ERPDLIILDVMMATWDEGFEVVQKIRNNPKLAATPIIMLTAVSQQMGIRYAKDKETLP
                     VEEFIEKPVMPGTLLNIIKKYI"
BASE COUNT         1387 a         1021 c          965 g         1263 t
ORIGIN      
        1 accgagtccg aaatcgaaaa tctcggattt attatccaaa aacggttacc ggaatccggt
       61 gactggttat tagttgcgga ttacacaact tgcgaggcgc tggctgggca cggcagtact
      121 tctgaagcac atgagtattc ctacaccgac gcggcggtca tcccgggtgc aacctatctt
      181 tatcgcctcg gcgatgtgga ttacaaagga aaagtaacct ggcataaaga ggtcgaggtt
      241 aaagtcgagg ctgaaaatgc gcaagttcca ctagtatttg gattgaagcc ggcctatccg
      301 aacccgttca acccgagtgt cactatacct tacggcttgg ccgaagacgg acagatgtcg
      361 ctgaaggttt acaacctgcg cggtgaatta gtcgaagtac ttataagcac ttatgctctt
      421 aaaggcactt actcttacaa ctggagtccg cagaacctga gcgctgggat ttatctggtg
      481 cgcttacaat ccggcaatca tacatcaatg cagaaaatag tcttcgtgaa ataataaacc
      541 gcagagtgtg ccaagatttt aaaatctttt aagttttctt aagtactttt aagaaattaa
      601 aatatactcg agatttaccg tcgtaaacat gtattcatca tttccgcttt tttcattttg
      661 acttagaatc tcgcgtaatt atcttatgcg cgtcgaatgg caagtgattt ttccgataag
      721 cagaaaatta ccagcgctca cggcgccggc ggtaaatatt ctcacgacct gatcaaacaa
      781 gtattcctga aacatttcga caaccctcct ttacgcgaat tgctggactc ggcgattcta
      841 ccggaaaggc acggacgatt ggtcttcaca accgattctc acgtagttca accacttttt
      901 ttccccggcg gcgacatcgg taaactcgcc gtttcgggaa cggtaaatga tttggcagtc
      961 tgcggagcca agccactctg gctttcctgt ggaatgataa tcgaagaagg tctcgagatt
     1021 gaaacgctgg aaaaaatcgt gatttcaatg caaaaaaccg ctcaatcagc tggtgtacag
     1081 attgttaccg gcgatactaa agtcgtggga cacggcgaag ccgacgggct ttttatcaac
     1141 acagccggaa tcggctattt gccggaaaat cgcaatatcg ctaaatcgct gatcactccc
     1201 ggcgataaga tcatcctgaa tggctttatc ggcgatcacg ccgccgcaat cataaaagcc
     1261 cgccaccagt ttcagattga ccttgatatt atcagtgatt gcgcgccgct gaataacttg
     1321 actgaggttt tatacgaaaa tatcacgggc atccggatta tgcgcgatcc aacacgcggc
     1381 ggtctggcga ctactctgaa tgaatttttg gacggtaacc gcttcgggat gagaatcagc
     1441 gaggaatttg tcccgattcg caatgtcgtg cagggtttat gcgaaccgct gggtttcgat
     1501 ccattttatc ttgccaacga aggaaaagtc gtagtcgtag tggcagaaaa atcagctgaa
     1561 aaggctttgc aaacgatgcg ttcgcatcca ctcggccgga atgccgccct aatcggtgaa
     1621 gttatcgctc aaccggctgg acacgttcta cttaaaaccc gtattggcac tgagcgaatt
     1681 ctcgatatgc tgaccggcga aatgttgcca cgaatatgtt agattgtaac tctcgcaaag
     1741 tcgcagagac gcaaagaatc tatttcgttt tattttggtg tctttataaa tgagtatttc
     1801 tctgtattta ataaacagta aaaaactttg cgccttagcg tatttgcgag agattattct
     1861 ctgcgtcctg gcgagagaaa ttttaagagg attttaaaat ggacgaaaat gatatttcca
     1921 ggatcattgt agattgcgcc tatcatatcc atcgcgaact cgggcctggt cttttagaat
     1981 cggtttatga aaaagtattg atgtatgaac tcgaaaagaa aggccttaac atagacaatc
     2041 aggtcgcgat tcccgtcaga taccatgaaa tttcgttaga gttaggattc agagccgatc
     2101 ttattattga aaataaaatc attatcgaat taaaatctgt cgaagaaatc ctccccgttc
     2161 ataaaaaaca actcctgact tatttgaaac taactgggtt gaagctcggt cttctcataa
     2221 atttcaattc taacttaatc aaagatggaa ttacacggat tgtcaataat ctttaaagat
     2281 atctacttcc ctcacagaga cgcaaagaat ctattttgtt ttattttggt gtctttataa
     2341 atgagtattt ctttatattt aataaagagt aaaaaacttt gcgccttagc gtatttgcga
     2401 gagattattc tctgcgtcct ggcgagagaa attttaagag gattttaaaa tggacgaaaa
     2461 tgatatttcc aggatcattg tagattgcgc ctatcatatc catcgcgaac tcgggcctgg
     2521 tcttttagaa tcggtttatg aaaaagtatt gatgtatgaa ctcgaaaaga aaggccttaa
     2581 catagacaat caggtcgcga ttcccgtcag ataccatgaa atttcgttag agttaggatt
     2641 cagagccgat cttattattg aaaataaaat cattatcgaa ttaaaatctg tcgaagaaat
     2701 cctccccgtt cataaaaaac aactcctgac ttatttgaaa ctaactgggt tgaagctcgg
     2761 tcttctcata aatttcaatt ctaacttaat caaagatgga attacacgga ttgtcaataa
     2821 tctttaaaga tatctacttc cctcacagag acgcaaagaa tctattttgt tttattttgg
     2881 tgtctttata aatgagtatt tctttatatt taataaacag taaaaaactt tgcaccttag
     2941 cgtctttgcg agagattatt ctctgcgtcc tggcatcttt gcgagagatt attcctaacg
     3001 gttttacgag aaagcaatgc atgaactaaa aatcgcccaa aatataatcg ctatcgccgc
     3061 cgatgaactg gctcgacgtc agatttccac ggctgtcagg gtcatccact tcaaagccgg
     3121 tcagatgcac gccattattc ccgaaacact gatgttcaat ttcgacacca taaaatctga
     3181 aattccgcaa ttcgcacacg ccgttctgtc tatcgaaata attccgttac ggatcaaatg
     3241 ccatcaatgt caaaacattt ccgaactcga cgaaccggta ttcgagtgcc ggcattgtca
     3301 cggaaccgat ctggaaataa tcaacggcca agaaatgttc gttgacaata tcgagatgga
     3361 agatggatag cgatagaagt aattatgaca cagaggaaca cggagatttt aataattata
     3421 aaaattactc catgaacctc agtgaatcct ctgtgcgcct ccgtgtaacc caaaaggatc
     3481 aaactatgga aatcaagatt gtgcgccacg ttctcgaagc caatgacaaa ttagccgctg
     3541 aaatccgtca gcgcaccgca aattcaaaaa cattactcgt caatttaatg agttcacccg
     3601 gctccggcaa aaccactcta ctggaaaaat tgataccgat gctccatgca aaagcctaca
     3661 aaatcggcgt gatcgaaggc gacatcacca ccacgatgga cgccgaacgc ctccagcctc
     3721 tgaacattcc cattgtccag atcaataccg aacaatttgg cggcgactgt catctcggag
     3781 cggaagtaat tttaccggca ctcgacagtc tcgacctggc taaactcgat ttcgttttca
     3841 tcgagaatgt cggcaacctg gtttgcccgg cggaattcga taccggcgcc gacgtcaatg
     3901 ttgttgttct gagtgtgacc gaaggtgagg acaagccact caaatatcca ctaatgtttc
     3961 gggtctgtca tctggcattg attagtaaaa ccgacctact cccctacctc aatctgaatc
     4021 tggatttact gcaccaaaat atggtgaaaa tcaaccccca actcaccatt tttccgattt
     4081 cagctcagcg cggagacggc cttgccgatc tgcgagattg gttaattgaa cacaaacgct
     4141 gaatttcaga tgttgcagaa taaggtcaag cattttaact tcgaacgctt attaatacaa
     4201 aatgaggaaa gcaatgctta agaaaatatt agttatagac gacgatgttg acctgataga
     4261 agtcctgcgg ttgatgttgg agaaaaatgg ctatctcgta atagatgccc aaaacggcac
     4321 ccgcggctat caattagtcg aaaaagaacg ccccgatctc atcattctcg acgttatgat
     4381 ggcaacctgg gacgagggat ttgaagttgt ccaaaaaatc cgcaataatc ccaaattagc
     4441 cgctactcct attattatgc taactgccgt tagccagcaa atgggcattc gatatgccaa
     4501 agataaggaa accctgccgg ttgaggaatt catcgagaaa cccgttatgc cgggtaccct
     4561 gctgaacata attaaaaagt atatctaacc tgcttaattc ataaatccat ataaagttgt
     4621 tgataaaatc cggttt
//
LOCUS       DAOSMP010000091         4559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_19041_length_4559_cov_20.215586, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000091 DAOSMP010000000
VERSION     DAOSMP010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19041_length_4559_cov_20.215586"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1318
                     /locus_tag="PKX09_09585"
     CDS             137..1318
                     /locus_tag="PKX09_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU18111.1"
                     /translation="MDLFKKCYAYKDAQIARENGYYPYFIPFSGGDDTVMIYKGQPKI
                     MIGSNNYLGLTHHPKVVEAAQKALNEYGTGRTGSRFLNGTLDIHEEFERELAEFTNKE
                     AALIFSTGYLVNMGVISSLLHKKDYAIIDRLNHASIVDGCRFSGANTVRYRHNDMSDL
                     AKVLKNVPDNSGKLVIVDGIFSMEGDIANLPEIVQIAKASGARIMVDDAHSLGVLGPN
                     GNGTAAHFGLSDQVDLIMGTFSKSFGAVGGFVSGEFEVIDYIKHIARSMIFTASLPPS
                     VVASTRAALQIIKSEPERRQRLIDIGNYMRREFKRLGFNTSTSETPVIPIVIGDDMKT
                     FTFWKMLFEAGVYTNAVVSPAVPSNSSRLRTSYIATHTQEQLDFVLDKFAQIGKQLAI
                     I"
     gene            1387..3090
                     /locus_tag="PKX09_09590"
     CDS             1387..3090
                     /locus_tag="PKX09_09590"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOU18112.1"
                     /translation="MSVLKLMITFTRQIKWASLIAICAVLIGCAAQGGPGGGPVDRTG
                     PVLLSSFPQDGATNVDLNSDLILNFSEPIDARITDGILQVTPNLSTAPQVKVKHRKVI
                     VKLNEPLQPDRTYIFNFGRNLKDYQNNATAQEIKIAFATGDSIDQGMIAGVVTDIPTN
                     TKTEVWFYKKNTVFPDSLWLAEPDYIISVDKDGNYQATNLPVGEYRALAVSGERPRPK
                     FVSENDLLALPQTEPLIIKNRHDYLENINFRLSKRYLKPFRLINAKPIDGYLELNFSR
                     PLMDTRLMPANFVFADEKVIVKHAWISEEQPTRIMLLADSLTPQSEHQISVSGIRDVE
                     ADTLPIDGRTAQFIWTTCSDSLKPKIISTNPPANATNVELSTAIQINLSEPVYQDTLA
                     QNIALFYYDTVNVAIHSSWIDANSLIIEPEKPLISASNYSLQINCRNWCDYAGNFFQD
                     SLFILKFSTIDQNLFGSISGKIISRDGGNLKNLIIDAQLTGSDKFFRQTRPDSTGFYE
                     FIDLLPGKYKFSIWEDRNCNGKYDFGRLAPYLPAEPYRTYQDEINVRSRWETAEVNWS
                     Y"
     gene            3241..4308
                     /locus_tag="PKX09_09595"
     CDS             3241..4308
                     /locus_tag="PKX09_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOU18113.1"
                     /translation="MYSVIMAGGIGKRFWPRSRRERPKQLLDVIGETTMLQMTYERLK
                     SVSDEKKIFIITAQNMKESILKTLKGFPEKNIICEPYGKNTAPAIGLATALIVNKDPE
                     AVIGVFPADHYISNIDEFKKSIGIGFRFARDHTALVTFGIKPTRPATGYGYIQIDKSV
                     KVIEDSVYKVKTFAEKPNLSTAQRFLESGEFLWNSGMFIWKGINILNAIKNFIPELFA
                     CLQSIAKSIDTPEYESTLKRHWAMIQSISIDYGILEKAKNVYVVQGNFDWSDVGSWDA
                     VYDLKTKDKNGNVLIGNVATLDTHGCYFYSRKNLLAAVGVSDLIIVQSKDSILVVNRG
                     DSERVKDLVEMLAREGFADHL"
     gene            4314..>4559
                     /locus_tag="PKX09_09600"
     CDS             4314..>4559
                     /locus_tag="PKX09_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18114.1"
                     /translation="MPKKLSNKRLQELRTECEHLLERLGYIVIYGLGNFKEGACLVQS
                     EKKIVINQYTPLDLQVDFLLKILKSQDLSGVFILPALR"
BASE COUNT         1306 a          968 c          978 g         1307 t
ORIGIN      
        1 gaatcacaag ctttaaatta taccaggtga gcgccacact atttgttatg cttccctcag
       61 ttatttatca atttgcaaat caatatatcc cactttaaat ttgcaacgca tattgaaatt
      121 tattacggtg tgaacaatgg atttatttaa aaagtgctac gcttataaag atgctcagat
      181 tgcgcgtgaa aatggatatt atccctattt tataccattt tcgggtggtg acgacacggt
      241 gatgatttat aaaggtcagc ccaaaataat gattggttct aataattact taggactgac
      301 tcatcatcca aaagtcgtcg aagccgctca gaaggcactc aacgaatatg gcaccggccg
      361 caccggtagt cggtttttga acggaactct ggacattcat gaggaattcg agcgggaact
      421 ggctgagttc accaacaaag aagccgctct gatattttca accggttatc tggttaatat
      481 gggagttatt tcaagtctcc tgcacaaaaa agattatgct atcattgatc ggcttaatca
      541 tgcaagcatc gtggatggtt gccgtttctc cggggctaat accgtccgtt atcgccataa
      601 cgatatgagc gatctggcta aagtcttgaa aaacgtgcca gacaatagcg gaaaattagt
      661 aattgtggac ggcatctttt caatggaagg cgatatcgcc aacttgcccg aaattgtaca
      721 gattgctaaa gcttccgggg ctagaattat ggttgatgac gcccactctt tgggtgttct
      781 gggacctaat ggtaatggca ctgcggctca ctttggtttg agtgatcagg ttgatttgat
      841 tatgggcact ttctctaagt cattcggcgc tgtcggcggc tttgtgtccg gcgagtttga
      901 agttatagat tacatcaaac acatcgcccg gagtatgatt tttaccgcca gtctgccgcc
      961 gagtgtggtg gcatctaccc gggccgctct ccagatcatc aaatctgaac ccgaacgccg
     1021 tcagcgatta attgatattg gtaattatat gcgccgggag ttcaagcgtt tgggatttaa
     1081 tacctcgact agcgagaccc cggtgattcc gattgttatt ggtgacgata tgaaaacttt
     1141 cacattctgg aaaatgttat tcgaagccgg tgtttatacc aatgccgttg tcagtccggc
     1201 agttcccagc aactcttcgc gccttcggac cagctatatc gccactcata cccaggaaca
     1261 gctggatttt gtgttggaca aatttgccca aataggtaaa caattagcca ttatttagtg
     1321 acaattaaac cggttaattc gaaccggaac gattccctac atttctgttc taataaattt
     1381 agtataatgt cagttctcaa attaatgata acctttacac gccagataaa atgggcatct
     1441 ctgatagcga tttgtgcggt tcttatcgga tgtgcggctc agggcggtcc tggtggggga
     1501 ccagtcgatc gcaccggtcc ggtgcttttg tcaagttttc cacaagacgg cgctaccaac
     1561 gttgatctta actctgatct catcttgaat ttttctgagc caattgatgc acgtataact
     1621 gatgggattt tgcaagtaac accgaacctc tcaactgcgc cccaggtaaa agtcaaacac
     1681 cgtaaggtga tcgtcaaact aaacgagccg ttacaaccag atcgcaccta catcttcaat
     1741 tttgggcgca atctgaaaga ttatcagaac aatgccaccg ctcaggaaat caaaattgcc
     1801 tttgccaccg gcgactcgat tgaccaagga atgatagctg gagtcgtgac tgacattcca
     1861 actaatacca aaaccgaggt ttggttctat aaaaaaaata cggtttttcc cgattccctc
     1921 tggcttgctg agccggatta tattatttcg gtggataaag atggcaatta tcaggcgact
     1981 aatttaccgg tcggcgaata tcgcgctctg gcggtttccg gtgaaaggcc gcgtcctaaa
     2041 ttcgtctccg aaaacgatct gctggcactg ccccaaactg agccgcttat tattaaaaac
     2101 cggcacgatt atcttgaaaa tatcaacttt cgcttatcaa aaaggtattt gaaaccgttc
     2161 cggctgataa acgctaaacc gattgatggt tatttggaat tgaacttctc tcgccctttg
     2221 atggatactc gattgatgcc agccaatttt gtttttgcgg atgaaaaggt tatagtaaaa
     2281 catgcctgga taagtgagga gcaaccgacc cggattatgt tgttggctga cagtcttacc
     2341 cctcaaagcg aacaccagat tagcgtatcg ggaatcagag atgtagaagc tgatactctt
     2401 cccattgacg gccgcactgc ccaatttatt tggacgactt gctcggattc cctaaagccg
     2461 aaaattatct cgacaaaccc accagccaac gccaccaatg ttgaattgtc aacggcaata
     2521 caaatcaacc tgagtgaacc ggtttatcaa gatactttag cgcagaacat tgcccttttt
     2581 tactacgata cggtaaacgt tgcaatccat tcatcctgga ttgacgccaa ttctttgatc
     2641 attgaaccgg agaagcctct aatatcggct tccaattatt cactacagat caattgccga
     2701 aattggtgcg actatgccgg taattttttt caggattctt tattcatctt gaaattcagt
     2761 acaatagatc aaaatttgtt cggcagtatt tcgggtaaaa ttatttccag agatggcggt
     2821 aatctcaaaa atctgattat tgatgctcaa ctcaccggct ctgataaatt tttccgtcag
     2881 actcggcctg acagtaccgg attttacgaa ttcatagatt tactacctgg aaaatataaa
     2941 ttttccatct gggaagaccg caattgcaat ggaaaatacg acttcgggcg cttagcaccc
     3001 tatctcccgg cggaaccgta tcgaacatat caagacgaaa ttaatgtacg ttcccgctgg
     3061 gaaacagccg aagttaattg gagttactaa tgactgcggt gaattaccag aaaagattaa
     3121 aggctgataa tatcctgtta tattctttag cagactcact tttattttat aaaaattcag
     3181 agtggatttc cttgcgcagt cttttgtgaa attttttagt ttaaatagga gtattcaaaa
     3241 atgtattccg taattatggc aggtggaatt ggtaagcgtt tctggcctcg cagtcgccgc
     3301 gaacgtccga agcaattatt agatgtcatt ggtgaaacca ctatgcttca gatgacttat
     3361 gaacgattga aaagtgtttc agatgagaag aaaatcttta tcattactgc ccaaaatatg
     3421 aaagagagca ttttaaaaac cttaaaaggc tttccggaga aaaacataat ttgtgaacct
     3481 tatggtaaaa atacggcgcc agctattggt ctggctacag ctttaatcgt taataaagac
     3541 ccggaagcgg ttatcggcgt ttttccggct gatcattata tcagcaatat cgacgagttt
     3601 aaaaaatcaa tcggcatcgg ttttcgattc gccagggatc ataccgctct tgtaaccttt
     3661 ggaattaaac cgactcgtcc ggcaactggc tacggttata tccagattga caaaagcgtc
     3721 aaagtcatcg aggattcagt ttataaggtg aaaacctttg ccgaaaagcc caatctatct
     3781 accgcccaaa gatttctgga aagcggcgaa tttctctgga acagcggtat gtttatctgg
     3841 aaaggcatca atattttaaa tgccataaaa aacttcatcc ccgaattgtt tgcttgccta
     3901 caatccatcg ctaaatctat cgacacgccc gaatatgaat ccactctgaa acgccattgg
     3961 gcgatgattc agtcaatttc aattgattat ggaattctgg aaaaagccaa gaacgtctac
     4021 gttgttcagg gaaatttcga ctggagcgat gtcggttcat gggacgctgt gtatgactta
     4081 aaaaccaagg acaagaacgg taatgttttg attggcaacg tagctactct ggatacccat
     4141 ggttgttatt tctattcgcg gaaaaatttg ttagcggccg tcggagtgag cgatttaatc
     4201 atcgtgcaat ctaaggattc cattctggtc gtaaatcgcg gtgactcaga gagagtcaaa
     4261 gacttagtcg aaatgctggc gcgggaaggc tttgctgatc acctgtaaat aatatgccta
     4321 agaaattatc gaataaacgt ctccaagaat tacgaaccga atgtgaacat ttactcgaac
     4381 gcctcggtta tatcgttatt tacggactgg gaaactttaa ggaaggcgct tgtctggtgc
     4441 agagtgaaaa gaaaattgta atcaaccaat acactccgtt ggatttgcag gttgactttt
     4501 tactaaaaat actaaaaagt caggatttat ccggcgtttt cattttaccg gctttgcgc
//
LOCUS       DAOSMP010000092         4547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_19112_length_4547_cov_17.292075, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000092 DAOSMP010000000
VERSION     DAOSMP010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19112_length_4547_cov_17.292075"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..980)
                     /locus_tag="PKX09_09605"
     CDS             complement(6..980)
                     /locus_tag="PKX09_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18115.1"
                     /translation="MTITQKIILTISLAFVTLSLINCAKEVIKVIDDRDSPVILTEPD
                     VEIAANRAMIFWTTDEPCSVSVKYFVIGNADTLTASGREYRQNHQVLLSNLLPNTIYR
                     FFTLSYDVSGNFTKTKLDSFTTTLDTTYFYSHGWDLYAAGDYTDACEAFQQYLTYYPN
                     DLAGLTALGWSQLQDSLIDSSIISFNSALNLDINYPDALAGLTVALYHNFHLYAMEIP
                     AVRLLATDSLYVFSHDERYDHRVIRLMLADFYNMDGRFENTQQIIDLLYPANGLNPDD
                     ANTWKIDDGEEILTFDTYESALTSLIEYLKWIWWEPVGLPKVTITCFS"
     gene            1086..1310
                     /locus_tag="PKX09_09610"
     CDS             1086..1310
                     /locus_tag="PKX09_09610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOU18116.1"
                     /translation="MASAKETEDKNISRRNFLKKLAATAVFTVPTIQSFSLLQTQPDG
                     GWWWWTGHHHHHAPGPGEPTPPLPPPPPGL"
     gene            complement(1326..2249)
                     /locus_tag="PKX09_09615"
     CDS             complement(1326..2249)
                     /locus_tag="PKX09_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOU18117.1"
                     /translation="MRKRVILNFFVCGLITAGFLSRCEKIIYTYDDQKPQITTPLTIS
                     VTDSSAEIYWETDEACHIKIKYGLTTDYGLEYEDPENCEIHLATLRGLEPHALYHLQV
                     YCWDFAANGPVKSPDTTFCTLANESSYLREGWQFFREKNYSAAKTAFDSAHQINPFLA
                     EIQAAYGWLQIRTNDLDSARQNLTAAYNLDHYLPITLAGLAVISMIDDVPLAAINYVK
                     AVLSLDQQWEYRYNPEISWKSLLLIRAQAYFQTNQMNLAQADLDSLWPQNGLNPDLPS
                     SWVVNKQSYDTYESAFGAALNYLIANPEKMG"
     gene            complement(2259..>4547)
                     /locus_tag="PKX09_09620"
     CDS             complement(2259..>4547)
                     /locus_tag="PKX09_09620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU18118.1"
                     /translation="WSFDDQDSPCLDTGDPNDDYSREPEDNGDRINIGCYGNTPQASK
                     KKSACPMTAVYKNFPNNKWVMIGVPIAPNEGNPPGDPLAIFGDDFGGQMPDGTNWTCI
                     HWVTEDSVMEYYEYGDGTIYQPPTPYPGLGYFVWQNTGAPVDVDVRGCPVDHCLLDVA
                     QAPYVDWESEYGVALGFNQFANPYYFTIDWSNTEIFKYASADKTGTPVKYTLSEAAAN
                     GWISQYAYTWDYNLSQYNIVVPANTHVDSISVWQGFYFTQIDSVSYLRLNIPNRRVLE
                     KPSPISGNLQSFGEKYTYRSSSVSPEWDWFLKIGVVSKELKLQDTENGIGTAAAAKDG
                     FDGLDALEMLGTDVAGNYLQFKFVIDRAPEIAYDLHAPFETSSTWKIQVETPAANKQK
                     TAEIVWSQIRLVPQNLKFSLLANDNSTVLVEDLRTATSYKFKIKDELTTFYLKVDKVN
                     DSVAPTFSFLFISNPLAPNDLTFFVIPSEPLESITATVNEASVNLTPVASPPNIYYGK
                     YYISSSGSVNIAVSGWDASGNSGSGSATLQALLAKSDATEKITLSDGGIVIELPAGSL
                     SEKTAVYATRCAMDLSLLDENTPIGEPLYIGPDNVKFNQKAVLHVSDLAESENVGLYR
                     LTDGAWEFVGQYQNSGVPLNSTGTYGFFSHKADQSQPDNTPLVFAINNCYPNPFNNTT
                     VIRYTVETYGKVRLTVYNLLGQTVRNLVRENQYAGAYLVTWDGRNNNGKQVASGIYYL
                     KMEVLNGDQVLFQSSKKLMLVK"
BASE COUNT         1235 a         1029 c         1071 g         1212 t
ORIGIN      
        1 gaattttagc taaagcaagt tattgtgact ttgggtagtc cgaccggttc ccaccaaatc
       61 catttcaaat attcgatcag gctggtcaaa gccgattcat aggtatcaaa agtaagaatc
      121 tcctcgccat cgtcgatttt ccaggtattt gcatcatccg ggtttaatcc attagccgga
      181 tataataaat caataatctg ctgagtattt tcgaaacgcc cgtccatatt atagaaatca
      241 gccagcatca aacgaatcac gcgatgatca tagcgttcgt catgactaaa aacatagagc
      301 gaatcggttg ccagtagcct cacagctgga atctccatcg cgtagaggtg aaaattatgg
      361 tagagggcaa cggtcagtcc tgccagcgcg tcggggtagt taatgtccag attcagagcc
      421 gaattaaaag agataatact gctatcaatt aagctgtctt gcagttgact ccagcccagc
      481 gccgttaatc cggcgaggtc attcgggtaa taggtcaaat attgctggaa agcctcgcag
      541 gcgtcggtat agtcaccagc cgcatacaaa tcccagccgt gagaataaaa ataggtcgtg
      601 tctagggttg tagtaaaact atccagctta gttttggtga aattgccgga aacatcatag
      661 ctcagcgtaa aaaatcggta gattgtattc ggtaacaaat tactcaagag aacttgatga
      721 ttctgccggt attcccgacc ggaggctgtc agagtatcag catttccaat tacaaaatat
      781 ttaaccgaaa ctgaacacgg ttcgtcggtc gtccaaaaga tcatcgcgcg attagccgcg
      841 atttccacat cgggttcggt cagaattacc ggagagtccc gatcgtctat gactttgatg
      901 acttcttttg cgcaattaat cagactaagg gtgacaaacg ccagtgagat tgttaagatt
      961 attttttgag taatggtcat cacaagactc cgtgaattct gccgattgaa ttattctcta
     1021 ttgattatag caattaatca ctttatatta tgattagttt tttcaatatt aacaaggtac
     1081 ttaatatggc aagtgcaaag gaaactgaag ataaaaatat cagtcggcgc aatttcttga
     1141 aaaaactggc ggctacggcg gtttttacgg ttccgaccat tcaatctttc tcgcttttac
     1201 agactcaacc ggatggaggc tggtggtggt ggaccggtca tcatcatcat catgcccccg
     1261 gtcctggtga accgactccg cctttgcccc caccgccccc gggtttataa attcttcacc
     1321 gaacattatc ccattttctc cggattagct atcaggtaat ttaaagccgc tccaaaggcg
     1381 ctttcataag tatcataaga ttgtttattg accacccagc tggacggcaa gtcgggattc
     1441 aatccgtttt gcggccagag ggaatcgaga tcagcttgag cgaggttcat ttgattcgtc
     1501 tgaaagtaag cctgcgcccg aatcaagagc aggcttttcc agctgatttc cgggttataa
     1561 cgatattccc actgctgatc aaggcttaac accgctttta cataattaat cgcggcaagc
     1621 ggcacatcgt caatcatact gattactgcc aatcccgcca gagtaatcgg caaatagtgg
     1681 tcgagattat aggcggcggt caaattctgg cgggcggaat ccaaatcatt cgttctgatt
     1741 tgtagccatc cataagccgc ttgaatttca gctaaaaatg gatttatctg gtgggcggaa
     1801 tcaaaagcag tcttagcggc ggagtaattt ttctctctga aaaactgcca gccttcgcgc
     1861 agatagctgg actcattagc cagagtgcaa aaagtggtat cgggcgattt taccgggcca
     1921 ttggcggcga aatcccagca ataaacctgc aaatgataga gagcatgcgg ttccaagccc
     1981 cttagagtag ctaggtgaat ttcgcaattt tccgggtctt cgtattcgag accataatcg
     2041 gtcgtcaagc catattttat tttaatatgg caggcttcat cggtttccca gtaaatctca
     2101 gcggaactgt cagttaccga aatggtcagc ggcgtggtga tttgcggttt ctggtcatcg
     2161 taggtataga taattttctc gcatcgggat aaaaatccgg cagtgattaa gccacagacg
     2221 aaaaaattta ggatgacgcg tttcctcatt ttcctacctt acttaaccaa catcaatttt
     2281 ttactgcttt ggaacaagac ctgatcgccg ttgaggactt ccattttcaa ataataaatg
     2341 ccggaagcaa cctgtttgcc gttattatta cgaccgtccc aggtgactaa ataagctccc
     2401 gcatattgat tttcccgtac cagattccga acggtttgac ccagcagatt gtaaacagtc
     2461 aggcgcacct tgccgtaagt ctcgacggta taacgaatca cagtcgtatt attaaaggga
     2521 ttgggatagc aattgttaat ggcgaaaacc agcggagtat tatccggctg ggattggtca
     2581 gctttatgcg agaaaaagcc ataagtgccg gtagaattga gcggcacgcc ggaattctgg
     2641 tattgtccca cgaattccca ggcgccgtcc gtcagtcggt aaagtcccac gttttcggac
     2701 tcagcgaggt cgctcacgtg cagaactgct ttttggttga atttaacatt gtctgggccg
     2761 atataaagcg gttcaccgat aggagtattt tcgtctaata ggcttaagtc cattgcacag
     2821 cgcgtcgcat agaccgcggt tttttcggat aatgaaccgg cgggcagttc tattacgata
     2881 ccgccatccg atagcgtaat tttttcagta gcgtccgatt tggcaagcag ggcttgcagc
     2941 gttgccgagc cgctccccga atttccggaa gcatcccagc cgctaacggc aatgtttact
     3001 gagccgctgg aggagatata gtatttgccg tagtaaatat tgggcggcga agccacggga
     3061 gtcaaattta ccgacgcttc attaacggta gccgtgatcg attccagcgg ttcggaaggt
     3121 attacgaaaa aggttaaatc atttggcgcc agcggattgc tgataaaaag aaaggaaaag
     3181 gtcggggcaa cgctgtcatt tactttgtca accttgagat aaaaggtagt gagttcgtcc
     3241 ttgattttaa atttatagga agttgctgtt cttaaatcct cgaccaaaac ggtgctgttg
     3301 tcgttagcta atagtgaaaa tttcaggttt tggggcacca ggcgaatctg cgaccagacg
     3361 atctcagccg ttttctgctt gttagcggct ggagtttcca cctgaatttt ccaggtgctg
     3421 gatgtctcga acggagcatg caagtcatag gcgatttccg gggcacggtc tatgacgaat
     3481 ttaaattgaa gataattgcc agcaacgtcg gtcccgagca tttcgagagc atccagacca
     3541 tcgaaaccat ctttagccgc ggcggcagtg ccgataccat tttccgtatc ctgtaatttc
     3601 aattctttgg aaacgacacc gattttgaga aaccagtccc attcgggcga gacgctcgaa
     3661 ctccgatagg tgtacttttc accgaaggat tgcagattgc cggaaatcgg agatggtttt
     3721 tccagaacgc ggcgattggg aatattgagt cgcagatagc tgaccgaatc tatttgcgta
     3781 aaataaaagc cctgccaaac cgaaatacta tcaacatgag tattggcagg caccacgata
     3841 ttgtattggg agagattgta atcccaggta taggcgtact gactgatcca gccgttagct
     3901 gcggcttcag agagagtgta ttttaccggg gtgccggttt tatcagcgga ggcatatttg
     3961 aaaatctcgg tattggacca atcaatcgtg aaataatagg ggttagcaaa ttgattaaaa
     4021 ccgagcgcaa ctccatattc agactcccaa tcaacataag gcgcttgggc aacgtctaag
     4081 agacaatgat caaccgggca acctctgacg tccacatcaa ccggagcgcc ggtattctgc
     4141 cagacgaaat agccgagccc gggataagga gtaggcggtt gataaatcgt gccatcacca
     4201 tattcataat actccataac ggagtcttcc gttacccagt gaatgcaagt ccaattggtg
     4261 ccatccggca tttgaccacc gaaatcatcg ccgaaaatgg cgaggggatc cccaggtgga
     4321 ttaccttcat taggcgcaat cggaactcca atcataaccc atttattatt aggaaagttt
     4381 ttataaaccg ccgtcatcgg gcaggcactt tttttcttgg atgcttgggg agtattgcca
     4441 taacaaccga tattaatgcg gtcgccatta tcctccggct ctcgactata atcatcgtta
     4501 ggatcaccgg tatccagaca gggtgaatcc tgatcgtcaa atgacca
//
LOCUS       DAOSMP010000093         4507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_19328_length_4507_cov_11.158580, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000093 DAOSMP010000000
VERSION     DAOSMP010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19328_length_4507_cov_11.158580"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PKX09_09625"
     CDS             complement(<1..900)
                     /locus_tag="PKX09_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOU18119.1"
                     /translation="MHIVDYFIICIYLIGILIVGLWFQRKASQGINSYFLGNRSLPWW
                     ALGASGMASNLDISGTMIIVALVYALGARGLYIEFRGGVTLIMAFLMIFMGKWNRRSK
                     VMTLAEWMEFRFGKGTEGRLARMITALTSILVTIAMVTYFAVGGGKFLDKFLGIPNFL
                     GLSSQFWAAAVLIAIAMIYTVASGLYGVVWTDVFQGSLIFLMILYVVIQVFHFDIPSE
                     FSVVMPLKDAAGGGFQFFPTTYQEWTDVLPKFKLSIDPSSVYSMYNLFGIAVLFYLLK
                     VVIEGCGGTGNYMIQRYYAAKDDR"
     gene            complement(975..3026)
                     /locus_tag="PKX09_09630"
     CDS             complement(975..3026)
                     /locus_tag="PKX09_09630"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU18120.1"
                     /translation="MKKATVLMLMLTLIAVSGLADVTVNYIVNTSTLQGVTDSTHQVQ
                     ICGAEVGPEGVDKWYNLFLTWGDESPLAANIGGDYWKLTIAYPDSMIGWRMAYKVRYK
                     ANEDDAFTWENVTGGGNRMWVLPATDTTLAVAYVNNDFNPPFAPSDSVDILVRVNMEN
                     YEDSFSLLSVVGHWPFCDPAWTPGALPLTQEGTSDYWSRTIRIPQAVMDTVDRVQPLG
                     AEHPGVLMYRFAIGNDWNNSENLNGKYWPGNENRIIALHPGLEDTTLAWKYWNDNPPA
                     GFIAEDTVDITFTADLTNAIANKGFAVGDTILVRMGYFNSLSSTMDANLLRISGTYLY
                     STTFTDVPVVLNEPLYYQYYLIKDAVEQREVYFNFDYEGEDVTRAERRAYVLTGDAEI
                     NDIEDSETDPRRMPKFRNNQKLSQAVTVTIECDLRPAYYQVWAGSTLDDIQGTIDITP
                     AMLEANPDTIAKLGLCINGPMSNHGEGTWQTWGGTLASDATRRMYDDGTNGDETAGDT
                     IYTIVYNFTTDKTIGQEFKFGIGGGDNESSYGLNHIENIDDTNPTFTLEVQFGSINPN
                     FYSAWDFNARAPKVGIKNQNATIAKEFSLSQNFPNPFNPTTEIRFTIQEKSDVHLTIY
                     NLLGKVVNKVTYNNLDIGNYSYVWNSKDLNGNSVASGIYFYELRAGSKYHAIKKMVLM
                     K"
     gene            complement(3049..4062)
                     /locus_tag="PKX09_09635"
     CDS             complement(3049..4062)
                     /locus_tag="PKX09_09635"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOU18121.1"
                     /translation="MNRKLPTIISTVLISTIFLNAQNKEGTAAGQFLQIPVGGKAIAM
                     GGAFTALADDASAIYYNPGAISRISKNKLYVSQTNWLVGGKHQFIAAQVMATSNDIIG
                     IGVDYLDYGEREKITTVAEPDGTGEYWEASDLAFSLGYARNLTDRFSIGGSVKFIQQN
                     IWHVSATGFAADLGLLFKTQFNGLQIGASMRNFGSELQLTGRDLITQVDLDEEASGNN
                     ETIVAYLKTDKWPIPLTFTIGVAMPVINSNQFVLTLAGDVVRPTDNSQTLNCGAEAII
                     MKMISLRAGYQALFRENKQQGLTAGIGLKLPIQYADFYFDYSYQEFGFFGDLSTMSIS
                     IGF"
     gene            complement(4073..>4507)
                     /locus_tag="PKX09_09640"
     CDS             complement(4073..>4507)
                     /locus_tag="PKX09_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18122.1"
                     /translation="RFLKGDILHIETEIPFRNGDIYEFTTEIPNVDNEVAKSSLDDIQ
                     VVPNPYVVANTMESPLPPAITSGRGERRIEFRKLPNDAKVHIFTSAGAHVITLNHNGN
                     IHNGTLAWNVKSKENLDVAFGVYYYIIESSVGKKSGKLAVIK"
BASE COUNT         1353 a         1046 c          843 g         1265 t
ORIGIN      
        1 acgatcatct ttagcggcgt agtaacgttg aatcatatag ttgccggtcc ctccacaacc
       61 ctcgataact accttaagca aataaaacag tacagcgatt ccgaataaat tgtacatact
      121 atagacactg ctgggatcga tcgataattt gaatttgggg agaacgtcgg tccactcctg
      181 ataagttgtg gggaaaaact ggaatccacc tcctgcagca tccttcaaag gcataaccac
      241 agaaaattca gacggtatat caaaatgaaa tacctggata actacgtata aaatcattaa
      301 aaatatcagc gaaccttgaa atacatctgt ccacaccact ccatataatc cggaagcaac
      361 cgtataaatc atcgcaatgg cgatcagaac cgccgccgcc caaaattgcg acgataaacc
      421 caaaaaattt ggaataccta aaaatttgtc taaaaatttt cctccaccca cagcaaaata
      481 agttaccatc gcaatcgtga ccagaatcga ggtcaaagcc gtaatcatcc gcgctaagcg
      541 cccctctgta cctttcccaa aacggaattc catccattct gctaaagtca tcacttttga
      601 acgccggttc catttaccca taaaaatcat taaaaatgcc ataatcaagg taacaccccc
      661 ccgaaattcg atatataaac ctcgggctcc taaggcatag accagggcaa caataatcat
      721 agttccgctt atatcaagat tggatgccat gccggatgct cctaaagccc accaaggcaa
      781 tgaccgatta cccagaaaat atgaattaat accctgagag gctttacgct ggaaccataa
      841 acccactatg aggattccta tcagataaat acagataata aaataatcta ctatatgcat
      901 ctggcgatct ccttaatgaa caatcaaaga aaaagaggaa ggacggaaaa tccgtccttc
      961 ctcttaagag caacttattt catcaaaacc atctttttga tagcatgata tttactacca
     1021 gcccgtagtt catagaaata aataccggag gcaacactgt tgccgtttag gtctttacta
     1081 ttccagacgt agctgtaatt gccgatgtcc aggttattat aagtaacctt attgacaacc
     1141 ttaccgagca ggttgtatat cgtcaaatga acatcgcttt tttcctgaat tgtaaaacga
     1201 atttctgtag ttggattgaa tggatttgga aaattctgag agagggagaa ttccttggca
     1261 atagtggcat tttgattttt gattccgacc ttaggtgctc tggcgttaaa atcccaagcc
     1321 gaatagaaat tcggattgat cgagccaaac tgaacctcca gcgtaaaagt cggattcgtg
     1381 tcatcaatat tttctatatg gttaagacca taactgcttt cattatctcc gccgccaatc
     1441 ccgaatttga attcctgacc gatggttttg tcagtggtaa aattgtatac aatcgtataa
     1501 atggtatcac cagcagtctc gtcaccatta gtcccatcgt catacattct gcgagtagcg
     1561 tcactggcta acgttccacc ccaagtctgc caggtacctt ctccgtgatt ggacatcgga
     1621 ccattgatac ataaacccaa ttttgcaatt gtatccgggt tggcttccaa cattgccgga
     1681 gtaatatcaa ttgtgccctg aatatcatcg agagtcgaac cagcccagac ttgataatag
     1741 gctggacgca ggtcgcattc aattgttacc gtgacagctt gactaagttt ctggttattc
     1801 ctgaattttg gcatgcgacg tggatcggtt tcgctatcct cgatgtcgtt gatttcagca
     1861 tcgccggtta ggacgtatgc acgtctttca gctcgcgtaa cgtcttcgcc ttcataatca
     1921 aaattgaaat atacttcacg ttgttctacg gcatctttta taagataata ctggtaataa
     1981 agcggttcgt taagaactac tggaacatct gtaaaagtgg tcgaatatag gtaggttccg
     2041 gaaattcgaa gcagattagc atccattgtt gaagacagac tgttaaaata acccatccgg
     2101 actaaaatgg tatcgccaac tgcaaaacct ttattggcaa tggcattagt taaatcggcg
     2161 gtaaaagtaa tatcaacggt atcttccgct ataaatccgg ccggtggatt gtcattccaa
     2221 tatttccaag ccaaggtcgt atcttccaag ccaggatgca gagcaatgat ccggttttca
     2281 ttacctggcc agtatttacc attcaagttt tctgaattgt tccaatcatt accgatcgca
     2341 aaacgataca ttagtacccc gggatgctct gcaccgagag gttgtactcg atcaacggta
     2401 tccatcacag cctgtggaat ccggatcgtt ctgctccagt aatcggaggt gccttcttga
     2461 gttaaaggca gtgcgccagg tgtccaggct ggatcacaga aaggccaatg gccaacaacc
     2521 gacaacagtg agaaactgtc ttcgtaattc tccatattta cccgaactaa aatgtcaaca
     2581 gaatcggatg gcgcaaatgg cggattgaaa tcattgttaa cataagcaac cgctaaagtc
     2641 gtgtcagtag ccggtaaaac ccacatacgg ttgccaccac cagttacatt ctcccacgta
     2701 aaggcatcat cctcattagc cttgtaacga accttataag ccatccgcca gccaatcatc
     2761 gaatccggat aagcaatcgt caatttccag taatctcctc caatattggc ggctagtggt
     2821 gattcatcac cccaggtcag aaaaaggttg taccacttat caacaccttc tggcccgact
     2881 tccgcaccac aaatctggac ctgatgggtc gaatcggtga ccccttgcag agttgatgta
     2941 ttgacaatat aattaactgt cacatcggct aatcccgaga cagcaattag agtcaacatc
     3001 aacatcaaaa cagtagcttt tttcatagct tactcctttc ttttgttgtt aaaaaccaat
     3061 tgatatagac attgttgaaa gatccccaaa aaatccaaat tcttgataag aataatcaaa
     3121 ataaaaatcg gcatattgga ttggcaattt caagccaata ccagcggtca atccttgctg
     3181 tttattttcg cgaaataaag cttgataacc ggcacgaaga ctaatcattt tcattattat
     3241 tgcttcagct ccacagttaa gtgtctggct attatccgta ggacgtacga catcacctgc
     3301 cagtgtcaaa acaaattgat tactattgat caccggcatc gcgactccta tagtaaacgt
     3361 taaaggaatt ggccatttat cagttttaag ataagcgacg attgtctcgt tattgccaga
     3421 ggcttcttcg tcaagatcaa cctgagtaat taaatcgcgt cctgttaatt gtaactcgct
     3481 accaaaattc cgcatgctgg caccaatctg gagtccgttg aattgagttt taaataataa
     3541 tcccagatca gcggcaaatc ccgttgcgct gacatgccag atattttgtt gaatgaattt
     3601 aacactgcct ccgattgaaa agcggtcggt taagttgcga gcatagccca agctaaaagc
     3661 taaatcactt gcttcccaat attcgccggt tccatccggt tccgctaccg tagttatttt
     3721 ttctcgctca ccgtaatcta agtaatcaac gccaattcca atgatatcat tgctggttgc
     3781 cattacctga gccgctatga attgatgctt ccctcccact aaccaattag tttgagaaac
     3841 atacaattta tttttactaa ttcgggaaat tgctcccgga ttataataaa ttgcactcgc
     3901 atcatcggcc agagctgtaa atgcgccacc cattgcaatt gccttcccac cgactggaat
     3961 ttgcaaaaat tgaccggcgg cagtcccttc tttattctga gcatttaaaa atatagtgct
     4021 aatcagtact gtcgaaatta tagtaggtaa ttttctattc atgttctcac cgctatttta
     4081 ttactgctaa tttgccggat tttttaccga ccgatgattc tatgatatag taataaaccc
     4141 caaatgctac atcgagattc tctttcgatt taacattcca ggctaaagta ccattatgaa
     4201 tattgccgtt gtgatttagg gtaattacat gcgccccggc tgatgtaaag atatgtactt
     4261 tggcatcgtt aggcaatttg cgaaattcaa tccgcctttc cccacggcca ctggtaatgg
     4321 caggcggtaa tggcgattcc atcgtattgg caaccacata aggattaggg acaacctgta
     4381 tatcatctaa gctcgatttg gctacttcat tatctacatt aggaatttca gtagtgaatt
     4441 cataaatatc accattacgg aaggggattt ccgtttcaat atggagaatg tcacctttta
     4501 gaaaccg
//
LOCUS       DAOSMP010000094         4495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_19392_length_4495_cov_11.131532, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000094 DAOSMP010000000
VERSION     DAOSMP010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19392_length_4495_cov_11.131532"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /gene="ribB"
                     /locus_tag="PKX09_09645"
     CDS             complement(<1..767)
                     /gene="ribB"
                     /locus_tag="PKX09_09645"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HOU18123.1"
                     /translation="MKFNSIDAAVEDLKKGRFVIVVDDENRENEGDFIMAAEKVTPES
                     VNFMAKYARGLLCAGLTEERAKELNLELMVQDNTALHNTRFTVSVDLKEGTTTGISAN
                     DRALTINALANPNTKPEDLGRPGHIFPIIARPGGVLRRAGHTEASVDLMRLAGLTPVS
                     VMCEIMDENGEMARGEALEKIAQTHNVKIITVQSLIEYRRRTERFIERVSEADLPTKF
                     GVFRIIIYVDRLTDKEHVALVMGDIKSDEPVIVRVHS"
     gene            complement(795..1436)
                     /locus_tag="PKX09_09650"
     CDS             complement(795..1436)
                     /locus_tag="PKX09_09650"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF009566.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOU18124.1"
                     /translation="MFTGIIEEIGEIRAISENVAGRRFQIAANRILNGINIGDSIAVD
                     GVCLTVEKVGKDYIEVQAVTETLVRTTLAKVPRGAKVNLERAMAASGRFGGHFVQGHI
                     DSVAPIVAFQHIGKAGTMRIAVPDSIRHYIVVKGSLAVNGISLTVAGIENEIIEIALI
                     PVTLTETNLGEKKIGDEVNLEVDILAKYIEKILERFEKEKLTFEKIKQWGFNQ"
     gene            complement(1436..2563)
                     /gene="ribD"
                     /locus_tag="PKX09_09655"
     CDS             complement(1436..2563)
                     /gene="ribD"
                     /locus_tag="PKX09_09655"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOU18125.1"
                     /translation="MEEDEKSRAQSGFFMNKDEKWLKRALELAEKGRGFTSPNPIVGA
                     VIVSGEKVVGEGFHSKFGAPHAEIKALEQAGESARGAKLYVNLEPCCFYGKTPLCTAT
                     IIKSGISEVYIGTIDPNPNVNGAGVKSLEQAGLKVQVGILEKEARYANRGFFAYIQKK
                     RPWVTLKMALTLDGFIADATGKSRWITGVEARKFVHEQRRLHDAVMVGMGTVYQDDPE
                     LLPDNRSGYIPYRVVLDEALNLPPRMKLVTDGFQRRTIIVTACQEKAEKIRQFESSGV
                     KIIQSSKDEFGWLDLPKTLAKLAEFGVTSIFCEGGSQVAGSLLNQRLVDELQIIIAPK
                     ILGEGLRAFSGFMQSLDQALMVEWEKVERLGADVLLQGKLV"
     regulatory      complement(2548..2671)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(2726..3964)
                     /locus_tag="PKX09_09660"
     CDS             complement(2726..3964)
                     /locus_tag="PKX09_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HOU18126.1"
                     /translation="MKFKAQLVEYFKSNQADVRSQIIAMVTEMVRQKTINVVTEKLPE
                     FPFLKMRGEEYRVAEIVQRELDQAGIPYEIFARAEGRPNVISRLGQNKSGQRLLMPAH
                     MDVVPAGEGWLTDPFEVVEKEGKLYGRGTLDNKGPLASILIAAQVLKKLGIDQQLTGE
                     LMVAALSDEEAEDPDGINYGIGYLVEENLINPTMAIVPDIGGNMLEIDVAEKGRTVIK
                     ITAIGKQAHGSTPEKGINAIFMMAKLVDELERLKFKYEVHPVLGHPTLNLGEIHGGVA
                     SNIVPGSCYIYLDIRSVPGQTPEILLEQIQECIAKVPNGNFKVEIISTNQPHAISPDV
                     ELVRKIQSNAKDFLNIEPQAIGQGGGTYAKTLCLNGIAAVGWGPGDDNAFHVANEYVE
                     IKQLVDFALMTCLLAVDLLV"
     gene            complement(4080..>4495)
                     /locus_tag="PKX09_09665"
     CDS             complement(4080..>4495)
                     /locus_tag="PKX09_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU18127.1"
                     /translation="YALGGAIGQAVGLVLSKYGMSDYNAFGATQIRVITGMIGFAVIF
                     LILKRFPLLTKVVRNRNAMKYTTIGAFIGPFLGVSLSLYAVQHTITGIASTIMAIVPV
                     LIIPLEVLIKKEKITFREVAGAIIAVSGVALYFIE"
BASE COUNT         1227 a         1158 c          844 g         1266 t
ORIGIN      
        1 tcagaatgca cccgaacaat caccggttca tccgatttta tatctcccat aaccaaagcc
       61 acatgttctt tatcggtaag tcggtcaaca taaataatga ttcgaaagac gccaaattta
      121 gtcggcaaat cggcttcact cacccgctcg ataaagcgtt ctgttcttcg gcggtattca
      181 atcaaactct gaacggtaat aattttaacg ttatgagtct gggcaatttt ctccaatgct
      241 tcaccgcgcg ccatttcgcc attttcgtcc ataatttcgc acatcacact gacgggtgtc
      301 aatccagcca atcgcatcaa atccactgag gcttctgtat gaccggcacg ccgcagaact
      361 ccgcccgggc gtgcaataat cgggaaaata tgtcccgggc gccctaaatc ttccggtttg
      421 gtgttcggat tagcgagagc attaatcgtc aaagcgcgat cattagccga aattccggtc
      481 gtcgtgcctt cctttaaatc cacactaacc gtaaagcgag tattatgcag agcggtatta
      541 tcctgcacca tcaattctaa attgagttct ttcgctcgct cttccgtcaa acctgcacac
      601 agcagaccgc gagcatattt agccataaaa ttgaccgatt ccggcgtcac tttttcagcc
      661 gccataataa aatcaccttc attttcgcga ttttcatcat ccacaacgat tacaaaccgc
      721 ccctttttta gatcttcgac ggcggcatct attgagttaa atttcattaa atcctcttaa
      781 tatcttaaaa tttactattg attaaatccc cattgtttaa ttttttcaaa agtcagcttt
      841 tctttttcaa atcgctccag aattttttca atatacttag ctaatatatc aacctcaagg
      901 ttaacttcgt cgccaatttt tttctcgccg agattagttt cggtaagcgt taccggaatc
      961 aaggctattt caattatctc gttctcaatt ccagccaccg tcaaactaat gccattaacc
     1021 gccaaagagc ccttcacgac gatataatgc cgaatcgaat ccggtacggc gattcgcata
     1081 gtacccgcct tgcctatatg ttggaaagcg acgatcggcg caacgctatc tatgtggccc
     1141 tgaacaaaat gtcccccaaa acgtccactt gccgccatgg cgcgttccag attaactttt
     1201 gcaccacggg gcaccttagc caacgtcgtc cgcacaagtg tctcagtgac cgcctggact
     1261 tcaatataat ctttaccaac tttttcaact gtcaaacaaa cgccatctac agcgatgctg
     1321 tcgccgatat tgataccgtt caaaattcga ttcgctgcaa tctggaaacg acgtccagcg
     1381 acattttcgg agatggctcg aatttcacca atttcttcga taattccagt aaacattaga
     1441 ctaatttccc ttgtagaagc acatccgctc caagacgctc gactttttcc cattcaacca
     1501 tcaaagcttg atccaatgat tgcatgaaac cagaaaatgc ccgcaaacct tcaccgagaa
     1561 tttttggcgc tataattatt tgtaattcat ccactaagcg ttgattcaac agcgatcccg
     1621 ccacctgact accgccttcg caaaaaatgg aagtaacacc aaactcagcc agtttagcaa
     1681 gagttttcgg caaatccaac cagccaaatt catctttcga actctgaata attttcacac
     1741 cggatgattc aaactggcga attttttccg ctttttcttg acaagcagta acgattatcg
     1801 ttcgccgttg aaaaccgtcg gttactaatt tcatccgcgg tggcaaattc aaagcttcgt
     1861 ccagcacaac tcgataagga atatatccag aacgattatc aggcaatagc tctggatcgt
     1921 cttggtaaac tgtacccatt ccaaccatca cggcatcgtg tagtcgccgc tgttcgtgaa
     1981 caaacttccg cgcttctacc ccggtaatcc agcggctttt accggtagca tcggctataa
     2041 aaccatccaa agtcagtgcc attttcagcg taacccaggg acgttttttc tgaatataag
     2101 caaaaaagcc gcgattagca tagcgcgctt ctttctctaa aataccgact tggactttca
     2161 gaccggcttg ttcgagactc ttaactccag cgccattaac attgggattg ggatcgattg
     2221 taccgatata aacttcacta attccggatt taataatagt cgcggtacag agcggggtct
     2281 ttccatagaa acagcacggt tcgagattaa cgtataattt cgcaccacgc gcggattcac
     2341 cggcttgctc taatgctttg atttcggcat gaggagcacc gaacttactg tggaaacctt
     2401 caccgacaac tttttcaccg gagacaatca ccgctcctac aatcggatta ggactggtaa
     2461 atccgcgtcc tttttccgcc agttccagag ctcgttttag ccatttctca tctttattca
     2521 taaaaaaacc cgattgcgct cgggattttt catcttcttc catccggact tttaccgtcg
     2581 gtgtctgagt ttcacagact ctgtcccgcc ttagcggaac tcgcggactt tcaccgccgg
     2641 tcaggattta caccctgccc cgaagacgcc cacaaattta taatacaatc ggcgaagtta
     2701 caagactgta aaaatgtttt cgagttcaaa ccagcaaatc taccgccagc agacaggtca
     2761 tcagcgcaaa atctactagc tgtttaattt ccacatattc attggcaacg tgaaacgcat
     2821 tatcgtcacc cggtccccag ccaaccgccg caataccgtt cagacaaagc gttttggcat
     2881 aggtaccgcc gccctgacca atggcttgcg gttcaatatt gagaaaatct ttggcgttgg
     2941 attggatttt tcggaccaat tcgacatccg ggctaatggc gtgaggttga ttagtagaaa
     3001 taatttcaac tttaaaatta ccgttaggaa ctttggctat gcactcctga atctgctcta
     3061 ataaaatctc cggcgtctga cccggcacgg atcgtatatc gaggtaaata tagcaagaac
     3121 cgggcacaat attagaagcc actccgccgt gaatttcacc taaattgaga gtcgggtgac
     3181 caagcactgg atgaacttca tatttgaact tcaaacgttc aagttcatca actaattttg
     3241 ccatcataaa aatagcattg atgccttttt ccggcgtaga gccgtgcgcc tgcttgccga
     3301 tagcggtgat cttaatgacc gtgcgacctt tttcagcgac atcaatttcc agcatattgc
     3361 cgccgatatc cgggacaatc gccatcgtcg gattaatcaa attctcttcc acaagatagc
     3421 cgataccata attgatcccg tctgggtctt ctgcttcttc atcagaaagt gccgcaacca
     3481 ttaattcgcc agttaattgc tgatcaattc cgagtttttt caaaacttgc gccgcaatca
     3541 ggattgatgc tagcgggcct ttattatcaa gcgtacccct gccatagagt ttgccttctt
     3601 tctcaacaac ttcaaatggg tcggttagcc aaccttcccc cgctggaacg acgtccatgt
     3661 gagccggcat caggagacgc tgtccgcttt tattctggcc taaacgcgat ataacattcg
     3721 gacggccttc cgctcgagca aaaatttcgt agggaattcc agcttgatcc agttcccgtt
     3781 gaacgatctc agctacgcgg tattcttcac cacgcatttt caggaatggg aattcaggca
     3841 atttttcggt tacgacatta atcgtttttt gtcgtaccat ctctgtgacc atagcgatga
     3901 tttgggatcg aacgtccgct tgattggatt tgaaatattc gactaattgt gctttgaact
     3961 tcataaagcc tcctgataat ttaaatgcga cattcttgtg agaaattact tgatttgcaa
     4021 atacttatca acagttaaaa atttaaaaat cctgacctat tcttaaaacg accataaatt
     4081 cactcaatga agtagagcgc tactccacta acggcgatga tcgctcctgc cacttctcta
     4141 aatgttattt tttctttctt tattaaaact tctaaaggaa ttatcaaaac cggcacaatg
     4201 gccataatcg tggaagcgat tccggttata gtatgttgaa cagcatagag cgagagcgag
     4261 acccccagaa atggtccgat aaatgctccg atagttgtgt atttcatagc gtttcgattg
     4321 cgaactactt tggtcaaaag aggaaagcgt ttcaagatta aaaatattac tgcaaaaccg
     4381 atcataccag taatgacccg aatctgagtt gctccaaagg cattgtaatc actcataccg
     4441 tatttgctaa gaaccaagcc gacagcctgt ccgattgcgc ctcctaaagc gtata
//
LOCUS       DAOSMP010000095         4492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_19410_length_4492_cov_12.391481, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000095 DAOSMP010000000
VERSION     DAOSMP010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19410_length_4492_cov_12.391481"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /gene="ribB"
                     /locus_tag="PKX09_09670"
     CDS             complement(<1..767)
                     /gene="ribB"
                     /locus_tag="PKX09_09670"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HOU18128.1"
                     /translation="MKFDSIDAAVEDIKKGRFVIVVDDENRENEGDFIMAAEKVTPES
                     VNFMAKYARGLLCVGLTEERAKELNLELMVQDNTALHNTRFTVSVDLKEGTTTGISAN
                     DRALTINALANPNTKPEDLGRPGHIFPIIARPGGVLRRAGHTEASVDLMRLAGLTPVS
                     VMCEIMDENGEMARGEALEKIAQTHNVKIITVQSLIEYRRRTERFIERVSEADLPTKF
                     GVFRIIIYVDRLTDKEHVALVMGDIKSDEPVIVRVHS"
     gene            complement(792..1433)
                     /locus_tag="PKX09_09675"
     CDS             complement(792..1433)
                     /locus_tag="PKX09_09675"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF009566.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOU18129.1"
                     /translation="MFTGIIEEMGEIRAVSENVAGRRFQIAANRILNGINIGDSIAVD
                     GVCLTVEKVGKDYIEVQAVTETLARTTLAKVPRGAKVNLERAMAASGRFGGHFVQGHI
                     DSVAPIVALQHIGKAGTMRIAVPDSIRHYIVEKGSLAVNGVSLTVAGIENEVIEIALI
                     PVTLTETNLGEKKIGDEVNLEVDILAKYIEKILERFEKETLTFEKIKQWGFNQ"
     gene            complement(1433..2560)
                     /gene="ribD"
                     /locus_tag="PKX09_09680"
     CDS             complement(1433..2560)
                     /gene="ribD"
                     /locus_tag="PKX09_09680"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOU18130.1"
                     /translation="MREDEKSRAQSGFFMNQNEKWLKRALELAEKGRGFTSPNPVVGA
                     VIVSGEKVVGEGFHSEFGAPHAEIKALEQAGESARGARLYVNLEPCCFYGKTPPCTEA
                     IIKSGIREVYIGTIDPNPKVNGAGVKSLEQASLKVQVGILEREARYANRGFFTFIQKK
                     RPWITLKMALTLDGFIADATGKSRWITGVEARKFVHEQRRLHDAVMVGMGTVYQDDPE
                     LLPDNRSGYIPYRVVLDEALNLPPRMKLVTDGFQRRTIIVTACQEKAEKIRQFESSGV
                     KIIQSSKDEFGWLDLPKTLAKLAEFGVTSIFCEGGSQVAGSLLNQRLVDELQVFIAPK
                     ILGEGLRAFSGFMQSLDQALMVEWEKVERLGADVLLQGKLV"
     regulatory      complement(2545..2668)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(2723..3961)
                     /locus_tag="PKX09_09685"
     CDS             complement(2723..3961)
                     /locus_tag="PKX09_09685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HOU18131.1"
                     /translation="MKLKAQLVEYFKSNQADIRSQIIALVTEMVRQKTINVVSDKLPE
                     FPFLKMRGEEYRIAEIVQRELDKAGIPYEIFARAEGRPNVISRLGQNKSGQRLLMPAH
                     MDVVPAGEGWLTDPFEVVEKEGKLYGRGTLDNKGPLASILIAAQVLKKLGIDQQLTGE
                     LMVAALSDEEAEDPDGINYGIGYLVEENLINPTMAIVPDIGGNMLEIDVAEKGRTVIK
                     ITAIGKQAHGSTPEKGINAIFMMAKLVDELERLKFKYEVHPVLGNPTLNLGEIHGGVA
                     SNIVPGSCYIYLDIRSVPGQTEEILLEQIQECIAKVPNGNFKVEIISTNQPHAISPDV
                     ELVRKIQSNAKDFLNIEPQAIGQGGGTYAKTLCLNGIAAVGWGPGDDNAFHVANEYVE
                     IKQLVDFALMTCLLAVDLLV"
     gene            complement(4077..>4492)
                     /locus_tag="PKX09_09690"
     CDS             complement(4077..>4492)
                     /locus_tag="PKX09_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU18132.1"
                     /translation="YALGGAIGQAVGLVLSKYGMSDYNAFGATQIRVITGMVGFAVIF
                     LILKRFPLLTKVVHNRDAMKYTTIGAFIGPFLGVSLSLYAVQRTITGIASTIMAIVPV
                     LIIPLEVLIKKEKITFREVAGAIIAVGGVALYFIE"
BASE COUNT         1236 a         1176 c          836 g         1244 t
ORIGIN      
        1 tcagaatgca cccgaacaat caccggttca tccgatttta tatctcccat aaccagagcc
       61 acatgctctt tatcggtaag ccggtcaaca taaataatga ttcgaaaaac accaaattta
      121 gtcggcaaat cggcttcact cacccgctcg ataaagcgtt ccgttcttcg tcggtattca
      181 atcaaactct gaacggtaat aattttaacg ttatgagtct gggcgatttt ctccaatgct
      241 tcaccacgcg ccatttcgcc attttcgtcc ataatttcgc acatcacact gacgggagtc
      301 aatccagcca atcgcatcaa atccactgag gcttctgtat gaccagcacg tcgcaggact
      361 ccacccggtc gcgcaataat cgggaaaata tgtcccggac gccctaaatc ttccggtttg
      421 gtgttcggat tagcgagagc attaatcgtc aaagcgcgat cattagccga aattccggtc
      481 gtcgtgcctt cctttaaatc cacactaacc gtaaagcgag tattatgcag agcggtatta
      541 tcctggacca tcaattctaa attaagttct ttcgctcgct cctccgtcaa acctacacac
      601 agcagaccgc gcgcatattt tgccataaaa ttgaccgatt ctggcgttac tttttcagcc
      661 gccatgataa aatcgccttc gttttcgcga ttttcatcat caacaacgat tacaaagcgt
      721 cccttcttta tatcttcgac cgccgcatct attgaatcaa atttcataat atcctcttaa
      781 catctgggaa tttattgatt aaatccccat tgtttaattt tttcaaaagt tagcgtttct
      841 ttctcaaatc gctccagaat tttttcaata tacttagcta atatatcaac ctcaaggtta
      901 acttcgtcgc caattttttt ctcgccgaga ttagtttcgg taagcgttac cggaatcaag
      961 gctatttcaa ttacctcatt ctcaattcca gccaccgtca aactaacacc attaaccgcc
     1021 aaagagccct tctcgacgat ataatgccga atcgaatccg gtacggcgat ccgcatagta
     1081 cccgccttgc ctatatgttg taaagcgaca atcggcgcaa cgctatctat gtgcccctgc
     1141 acaaaatgtc ctccaaaacg tccacttgcc gccatagcgc gttccagatt aacttttgca
     1201 ccacggggca ccttagccaa cgtcgtccgc gcaagtgtct cagtgaccgc ctggacttca
     1261 atataatctt taccaacttt ttcaactgtc aaacaaacac catcaacggc gatgctgtca
     1321 ccgatattaa tgccgtttaa aattcgattc gctgcaatct ggaaacggcg tcctgcgaca
     1381 ttctcggaga cggctcgaat ttcacccatt tcttcgataa ttccagtaaa cattagacta
     1441 atttcccttg taatagcaca tcggctccaa gacgctcgac tttttcccat tcaaccatca
     1501 aagcctgatc cagtgattgc ataaatccgg aaaacgcccg caaaccttca ccaagaattt
     1561 tcggtgcaat aaatacttgt aattcatcca ctaggcgttg attcaacagc gatcccgcca
     1621 cctgactacc gccttcgcaa aaaatggaag taacaccaaa ctcagccagt ttagcaagag
     1681 ttttcggcaa atccaaccag ccaaattcat ctttcgaact ctgaataatt ttcacaccgg
     1741 atgattcaaa ctggcgaatt ttttccgctt tttcttgaca agcagtaacg attatcgttc
     1801 gccgttgaaa accgtcggtt actaatttca tccgcggtgg caaattcaaa gcttcgtcca
     1861 gcacaactcg ataaggaata tatccagaac gattatcagg caatagctct ggatcgtctt
     1921 ggtaaactgt acccattcca accatcacgg catcgtgtag tcgccgctgt tcgtgaacaa
     1981 acttccgcgc ttctaccccg gtaatccagc ggcttttacc ggtagcatcg gctataaaac
     2041 catccaaagt cagcgccatt ttcagcgtaa tccaaggacg ttttttttga ataaaggtaa
     2101 aaaagccgcg attggcatag cgagcttccc tttccaaaat accgacttgg acttttagac
     2161 tggcttgttc gagactctta actccggcgc cgttaacttt gggattggga tcaatagtac
     2221 cgatataaac ttcacgaatt ccggatttaa taatagcctc ggtacagggc ggggtctttc
     2281 catagaaaca acacggttcg agattaacgt ataatctcgc accacgcgcg gattcaccgg
     2341 cttgctctaa cgctttaatt tcggcatgag gagcaccgaa ttcactgtgg aaaccttcac
     2401 cgacaacttt ttcgccagag acaatcacgg ctcctactac cggattagga ctggtaaacc
     2461 cacgcccttt ttccgccagt tccagagctc gttttagcca tttctcattt tgattcataa
     2521 aaaaacccga ttgcgctcgg gatttttcat cttctctcat ccggactttt accgtcggtg
     2581 tctgagtttc acagactctg tcccgcctca acggaactcg cggactttca ccgccggtca
     2641 ggatttacac cctgccccga agacgcccac aaatttataa tacaatcggc gaagttacaa
     2701 gactgtaaaa atgttttcga gttcaaacca gcaaatctac cgccagcaga caggtcatca
     2761 gcgcaaaatc tactagctgt ttaatttcca catattcatt ggcaacgtga aacgcattat
     2821 cgtcacccgg tccccagcca accgccgcaa taccgttcag acaaagcgtt ttggcatagg
     2881 taccgccgcc ctgaccaatg gcttgcggtt caatattgag aaaatctttg gcgttggatt
     2941 ggatttttcg gaccaattcg acatccgggc taatggcgtg aggttgatta gtagaaataa
     3001 tttcaacttt aaaattaccg ttaggaactt tggctatgca ctcctgaatc tgctctaata
     3061 aaatttcctc cgtctgaccc ggcacagaac gtatatcgag gtaaatatag caagaaccgg
     3121 gcacaatatt ggaagccact ccgccgtgaa tttcacctaa attgagagtt ggattaccaa
     3181 gcaccggatg aacttcatat ttgaacttca aacgttcaag ttcatcaact aatttcgcca
     3241 tcataaaaat agcattgatg cccttttccg gcgtagagcc gtgcgcctgc ttgccgatgg
     3301 cggtgatctt aatgaccgtg cgaccttttt cagcgacatc aatttccagc atattgccgc
     3361 cgatatccgg gacaatcgcc atcgtcggat taatcaaatt ctcttccaca agatagccga
     3421 taccataatt gatcccgtct gggtcttctg cttcttcatc agaaagtgcc gcaaccatta
     3481 attcgccagt taattgctga tcaattccga gttttttcaa aacttgcgcc gcaatcagga
     3541 ttgatgctag cgggccttta ttatcaagcg tacccctgcc atagagtttg ccttctttct
     3601 caacaacttc aaatgggtcg gttagccaac cttcccccgc tggaacgacg tccatgtgag
     3661 ccggcatcag gagacgctgt ccgcttttat tctggcctaa acgcgatata acattcggac
     3721 ggccttccgc tcgagcaaaa atttcgtagg gaatcccagc tttatccaat tcccgttgaa
     3781 cgatttcagc tattcggtac tcttcaccac gcattttcag gaatgggaat tcaggtaatt
     3841 tatccgaaac aacatttata gttttttggc gtaccatttc cgtgaccaga gcgatgattt
     3901 gggatcgaat gtccgcttga ttggatttga aatattcgac taattgcgct ttgagcttca
     3961 taaagcctcc tgataattta aatgcgacat tcttgtgaga aattagttga tttgcaaata
     4021 cttatcaaca ggtaaaaatt taaaaatccc gacatattct taaaacgacc ataaatttac
     4081 tcaatgaagt agagggctac cccaccgacg gcgataatcg ctcctgccac ttctctaaat
     4141 gttatttttt ctttctttat taaaacttct aaaggaatta tcaaaaccgg cacaatagcc
     4201 ataatcgtgg aagcgattcc ggttatagta cgttgaacgg catagagcga gagtgagact
     4261 cctagaaatg gcccgataaa tgctccgata gttgtgtact tcatagcgtc tcgattatga
     4321 actactttgg tcaaaagcgg aaagcgcttc aagattaaaa aaattactgc aaaaccgacc
     4381 ataccagtaa tgacccgaat ctgggtcgcc ccaaaagcgt tgtaatcact cataccatat
     4441 ttgctaagaa ccaagccgac agcctgtccg attgcgcctc ctaaagcgta ta
//
LOCUS       DAOSMP010000096         4358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_20173_length_4358_cov_21.297234, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000096 DAOSMP010000000
VERSION     DAOSMP010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20173_length_4358_cov_21.297234"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PKX09_09695"
     CDS             <1..702
                     /locus_tag="PKX09_09695"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOU18133.1"
                     /translation="YVHFARVYFDFFPLYEASDTQFDAIVECPDLNVLHNALARHRGV
                     VLVSFHFGNWEVCSDWFRRHDYQVAAVAKKMKNHLVDQMIFDARSQSGRNKEQIFYKS
                     KANSPRIWKYLRDENILYLIADQDARRDGIFVKFFDQWSSSHRGPALFALRQKAPLIA
                     ASCLMNTKGKYVIRLKELNTDVPPENKSDPVAWLTQQIAIYFEEQIRKCPEQYYWFHR
                     RWKTKPPPSFIEQNA"
     gene            695..1330
                     /locus_tag="PKX09_09700"
     CDS             695..1330
                     /locus_tag="PKX09_09700"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOU18134.1"
                     /translation="MPEIISLAKAADIDCQKVLAILQSGGIIAYPTDTIYGLGVDAFQ
                     PQAVQKLLQLKGRTAGKPISVLYADVPSLLSDFKHLNDYQRRVVKTLLPGKITLILPL
                     SSISIFPVEISADDSVGVRVISLEPMNRILRQYPHPITTTSVNPANAPPAHSAAEIIS
                     YFGDQISLILKYEIAADSLPSTLVKVGKSELEIIRIGAVTMAEIEEKMKQL"
     gene            1327..3171
                     /locus_tag="PKX09_09705"
     CDS             1327..3171
                     /locus_tag="PKX09_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOU18135.1"
                     /translation="MNIYRRVLKLVKPYWAVLTVSIITSLIYVALNSTSIWLTASFTK
                     VLFPATSDTETSQVIPVVPEKGKGSLNDTLKNYTDRLILRDTPLESLRALCLIIFLTF
                     LLKNVFLYIKNVSVGFVQLRMINDVRNKLYERLHDLSLSYFNRKRSGEITSIIMNDVN
                     AMRNSFTVSFDKLLVEPINILVFMVLLFIISWQLALLAVVILPVSGFIISKIGKSIRR
                     KSLRSSRQIAGIMAILDETLQGMRVVKAFAMEKFEIERFRKESRKYFNLVFRRKKLRE
                     FSSPINEVFGVFIGVLLLWFGGSLVLTGKGIDAEDFVRFAVMLFAIMNPIKSLTNVSM
                     EMQEGLASAQRVFSLLDEKPDVVEKPNAIEIKGFNDKIVYDNVSFKYETSRTEVLSGV
                     NLEIKKGEIVAIVGHSGAGKSTLVDLLPRFYDPTGGRVLIDGHDIRDLTFASLRSLMG
                     IVTQHTILFNDTIFNNIAYGMQDFDPEKVRAAAEAANALEFIEEFPEKFETMIGDKGL
                     RLSGGQRQRLAIARALLKNPPILILDEATSSLDSESEQKVQAAIENLMKNRTVLVIAH
                     RLSTIHNADKIVVLEHGRIVEIGTHNGLLSREDSIYRTFYETQFAKNE"
     gene            3183..>4358
                     /gene="selA"
                     /locus_tag="PKX09_09710"
     CDS             3183..>4358
                     /gene="selA"
                     /locus_tag="PKX09_09710"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOU18136.1"
                     /translation="MAGRENYPNDLKRLPSVQKVLDRLAQSESPIKPAILTTLVRQEL
                     NELRAAIRTGNFISPKSDDLIEVVAEKVAGRINALTKGYLQKVINATGIILHTGLGRA
                     PFGNEILPKMTELLSGYVNLEFDLDSGERGERLNLTNEKICLLTGAESSVVVNNNAAA
                     VWLALNSLADGREVIVSRGELIEIGGSFRLPDIMAKSGARLVEVGTTNRTYLQDYQKA
                     RTRKTSAILLAHSSNYRIEGFTTKPTASEITTWAHSQNLPVIFDLGSGALFSMENVEL
                     PYEPVVAEVVKQGFDLITFSGDKLLGGPQAGIIVGREKYIRCLRKNPLMRILRCDKTT
                     FFLLDYTLSQYLLDKPFPEIETYRLLTTKPAQLKAQAIQILNAIEPEIIKTLNLTVRE
                     "
BASE COUNT         1188 a          974 c          977 g         1219 t
ORIGIN      
        1 tatgttcatt ttgcgcgggt gtatttcgat ttctttccgc tttatgaagc ctccgacact
       61 caatttgatg ctatagtaga atgtcccgac ctaaatgttt tacataatgc cttagcccgt
      121 caccgcggtg tggtgctggt gtcttttcat tttggtaact gggaagtctg cagtgattgg
      181 tttaggcgcc acgactatca ggttgccgct gtagctaaaa agatgaaaaa tcatcttgtt
      241 gaccagatga tttttgatgc cagaagtcag agcggcagga ataaagagca aatattttac
      301 aaatcgaagg cgaattcacc ccgtatctgg aaatacctac gcgatgaaaa tattctctat
      361 ttgatagctg atcaagatgc ccgccgtgat gggatattcg ttaaattttt cgatcaatgg
      421 tcatccagtc accgcggtcc agccttattt gctttgcgcc aaaaagcgcc gctaatcgcc
      481 gcaagttgtt tgatgaatac taaaggcaaa tacgtaatca ggttgaaaga attgaataca
      541 gatgtcccgc ccgagaataa gagcgatccg gtcgcctggt tgactcagca aatcgcaatc
      601 tattttgaag aacaaattcg caagtgtccc gagcaatact actggtttca ccgtcgctgg
      661 aaaactaaac cacccccatc ttttatcgaa caaaatgcct gaaataattt cacttgccaa
      721 agcggctgat attgactgcc aaaaagtact tgctattttg caaagcggcg gcatcatcgc
      781 ttatccaacc gatacaattt acggtctcgg tgtggatgcc ttccaaccgc aagcagttca
      841 aaaattacta caattgaaag gtcgcaccgc tggaaaaccg attagtgtgc tatatgccga
      901 tgttccgtcg ctattgagcg attttaagca cttgaatgac tatcagagga gagtcgtcaa
      961 gaccttattg ccggggaaaa ttaccctgat tttgcctctc agctcgatta gtattttccc
     1021 tgttgagatt tccgctgacg actctgtcgg cgtccgggtc attagtctcg aaccgatgaa
     1081 tcgcatttta cgacaatatc cgcacccaat aacgaccacc agcgtgaatc cggctaacgc
     1141 gccgccggct cattccgccg ccgaaataat cagttacttt ggtgaccaaa tttcattaat
     1201 tttaaagtat gaaattgcag cagattcact accatcaacg ttggttaaag tcggtaaaag
     1261 tgaattggaa ataattcgga tcggtgccgt taccatggct gaaatagaag agaaaatgaa
     1321 acaattatga acatttatcg gcgtgtttta aaattagtta agccttattg ggcggtactg
     1381 acagtcagta taattacctc tttgatttat gtcgctctca acagtacctc aatctggctg
     1441 acggcttctt tcaccaaggt gctttttccg gcaacatctg ataccgaaac cagccaggtt
     1501 atacccgtcg ttccggaaaa aggcaaagga agtctcaacg ataccctgaa aaattatacc
     1561 gaccgattga ttctgagaga tacgccactt gaatctttgc gagctctttg cctgataatt
     1621 ttcttaactt ttctattgaa aaacgttttt ctatatatca agaatgtctc ggtcggtttc
     1681 gtccaactgc ggatgattaa tgatgtccgc aataaacttt acgagcgttt gcatgacctt
     1741 tcactgtcat attttaaccg caaacgaagc ggcgaaatta cctcaataat aatgaacgat
     1801 gtcaacgcta tgcgtaattc ctttacggta agttttgata aactactggt cgagccgatt
     1861 aatattctgg tttttatggt attgcttttc attatttcct ggcaattggc attactggcg
     1921 gtggtcattt tgccggttag tggttttatt atttccaaaa tcggcaagag tattcgacgc
     1981 aaatcactgc gatcttcccg tcaaattgcc ggcattatgg cgattctgga tgagactctc
     2041 caaggtatgc gcgtggtgaa agcttttgct atggaaaaat ttgagattga gcgttttcgt
     2101 aaagaaagcc gtaaatactt taaccttgtt tttcgacgca agaaactacg tgaattctct
     2161 tcgcctatta atgaggtttt tggcgtattt attggcgttc ttttgctctg gttcggtggt
     2221 agtctggttt tgactgggaa aggtattgat gccgaagatt ttgtccggtt cgccgttatg
     2281 ctgtttgcca ttatgaatcc aatcaaaagt ctgaccaacg tttcgatgga aatgcaagaa
     2341 ggcttggcat cagctcaacg ggttttttcc ttgcttgacg aaaaaccgga cgtagtagaa
     2401 aaaccgaatg cgatcgagat taaaggcttt aacgacaaaa tagtttatga caatgtctct
     2461 tttaaatatg aaacttcgcg gactgaggtt ctcagtggcg taaatctgga aatcaagaag
     2521 ggcgagatcg tcgccatcgt tggccatagt ggcgccggta aatccactct ggttgatctt
     2581 ctgccgcgct tttatgatcc cacaggtggc cgcgttctga ttgatggcca tgatattcgc
     2641 gacttgactt ttgccagcct gcgttcatta atgggaatcg ttacccagca tacgattttg
     2701 tttaacgata caatttttaa caacatcgct tacggtatgc aagatttcga tccggaaaaa
     2761 gtgcgtgccg cggcggaagc cgccaacgcc ttggaattta tcgaagaatt tcccgaaaaa
     2821 tttgaaacta tgatcggtga taaaggctta cgactttccg gcggccagcg ccagcgcttg
     2881 gcaattgctc gagctctact gaaaaatcca ccaatcttga ttctcgacga agccacttca
     2941 tccctcgatt ctgaatccga gcaaaaagtt caagccgcaa tcgaaaattt gatgaaaaac
     3001 cgtaccgttc tggtaatcgc ccatcggctt tctaccattc ataatgccga taaaatcgtt
     3061 gttttagagc acggacgaat tgtcgaaatc ggcacacata acggactatt gagtcgcgaa
     3121 gatagcattt accgcacttt ctatgaaacc caatttgcaa aaaatgaata agggcgaaac
     3181 ttttggcggg tagagaaaac tatccgaacg atctaaaacg gttgccgtca gtccaaaaag
     3241 tccttgaccg tctggcacaa tcggagtcac caattaaacc tgctatcctg acaacactcg
     3301 tccgccagga gctcaatgaa ctgcgtgcgg ctatccgtac tggaaatttc atttccccca
     3361 agagtgacga cctgatagaa gtcgttgccg aaaaagttgc cggtcggatt aatgcgctga
     3421 ctaagggtta tttacaaaag gtaatcaatg ccaccggaat catcctgcat actggactgg
     3481 gacgcgcgcc tttcgggaat gaaattttac caaaaatgac tgaattactt tccggatacg
     3541 taaatctgga attcgatctg gattccggcg aacgcggcga acggcttaat cttaccaatg
     3601 aaaaaatttg cttgctgact ggcgccgagt cgtcggtggt agtcaataac aacgccgcgg
     3661 ctgtctggct ggctttgaat tcgctggcgg atggtcggga agtcatcgta tcgcgtggcg
     3721 aattaatcga gatcggcggt agttttcgtt tgccggatat aatggcgaaa agtggggctc
     3781 ggctggttga ggttggcacg accaatcgca cttatctcca ggattaccaa aaagcacgca
     3841 cgcggaaaac cagcgcgatt ttgctggcgc attcctccaa ttatcgcatc gaaggcttta
     3901 ccaccaagcc gacggcatcg gaaatcacca cttgggcgca ttctcaaaac ctgccggtca
     3961 ttttcgacct cggtagtggt gcgctttttt ctatggaaaa tgtagaattg ccctacgagc
     4021 cggtcgtagc cgaggtagtc aaacaaggat tcgatctgat tacttttagc ggcgataaat
     4081 tgctgggcgg accacaagcg gggataatcg tcggtcgcga aaaatatatc aggtgcttac
     4141 gtaaaaatcc gctgatgcgc atcttacgtt gtgataagac gacatttttc ctgctcgatt
     4201 atactcttag ccaatatttg cttgacaaac cgtttccgga aattgagact tatcgtttgc
     4261 taacaactaa gcccgctcag ctaaaagccc aagctatcca gatcctaaat gctattgagc
     4321 cggaaattat taaaacgctc aacctaacgg tgcgtgaa
//
LOCUS       DAOSMP010000097         4354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_20192_length_4354_cov_19.383112, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000097 DAOSMP010000000
VERSION     DAOSMP010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20192_length_4354_cov_19.383112"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1421
                     /locus_tag="PKX09_09715"
     CDS             141..1421
                     /locus_tag="PKX09_09715"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOU18137.1"
                     /translation="MHAYSFQLYSLALLATIIIATVTAIVLWPRRKFKGVLWMILLEI
                     AAAEWAFGILFESAATTHRLKELWSAIAYIGTCSVPLFFFLFTVEYYRGRLQISKIAL
                     TFLITIPIFIIILAFTNPLHNLLWPNITINPATNIAIYSHGIVWWITYVYEYALIIIG
                     MYFLLKMAFESGSFYTPHNLTIIIGALIPIIGNLIYVFGPNPVPGLDWAPVGFALSGI
                     VITWAILRLQLFKLRPIAYSFLVDKMLDGMIILDTNNVIVDINPASANIFHKRPKQMI
                     GQKINRFLPQSIDLKNLLLAENPSQIEIALGKIQPDRIFELRLSILRDNYSQIIGKSL
                     LLRDITEKKKNENEREKLIRDLQNAMSQIKTLSGLLPICAHCKKIRDDKGYWHAVEEY
                     IHEHSDADFSHSICPDCLEKYYPEIAKKMKSSQS"
     gene            1498..1845
                     /locus_tag="PKX09_09720"
     CDS             1498..1845
                     /locus_tag="PKX09_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU18138.1"
                     /translation="MKAEGKNSEQDQYRFRILKTADLGNYQTGVIVSRTLIDRPAGNV
                     TFFAFDEGQRLSEHTAPFDALVNILDGEAEITIAGQSFSVKADEMIIMPANQPHAVKA
                     VKRFKMLLTMIKG"
     gene            complement(1968..3158)
                     /locus_tag="PKX09_09725"
     CDS             complement(1968..3158)
                     /locus_tag="PKX09_09725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU18139.1"
                     /translation="MSVKVLIKKDREKSLLRHHPWLFSGAIERIEGEPQLGETVELRA
                     QDGRFLGFGAWSPKSQIAVRTWSFNPDDEINADFFRRQLSLAIKHRESLFEPNKTDAV
                     RLVNAESDGLPGLIVDRYNDYLVVQFLSAGAEFWRETIVTVLAEIVPVTGIYERSDVE
                     VREKEGLELRRGLLQGNEPPELIEICENFRRFLVDIRNGHKTGFYLDQRDNRQKITEF
                     SAEREVLNCFAYTGGFAVAALSAGAIKVTNIETSADALELLNRNIALNGFAPARVENL
                     TDDVFQVLRKFRDQGRSFDLIILDPPKFAESKSQIERASRGYKDINLLAFKLLRPGGI
                     LFTFSCSGQISPELFQKIVADSALDAGKSAQILVWLNQSADHPTALNFPEGHYLKGLV
                     CVVE"
     gene            complement(3168..>4354)
                     /locus_tag="PKX09_09730"
     CDS             complement(3168..>4354)
                     /locus_tag="PKX09_09730"
                     /inference="COORDINATES: protein motif:HMM:NF020671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HOU18140.1"
                     /translation="RGQRAALNLVGVSKEQIERGMIVATLGAFVKTNLLTVYIESLPS
                     APPIEYNDQLRLHLGTGEYIVRIRLIGRDRLFPGKQGVAQIVLDQPISAGFRDHFILR
                     SYSPLVTIGGGIVLENSPEPLRKKDEPIAAELERLVCAGNEEWLKWLIFRQGEKLITI
                     EKLAQVLTVTVAEISPLLAKLVKENVIREREGKIIAAERLAECAEKITATLAEFHRQN
                     PLLPGLEKATLFGNLHFDEMLGDWLTEDLTQAGKIRVIGDKLAIAGFAPQLSERQTTR
                     QEQILAEITKSALTPPTLAELAEKLQTDLAEMRQLINLLVSQQEVVLIDRIYPFSAAA
                     VAEAETKLIEFLRGKSGATVSELKEVLGISRKFALPLLNYFDQKGMTARSGDKRRLGS
                     ID"
BASE COUNT         1224 a         1008 c          880 g         1242 t
ORIGIN      
        1 gaaaacatct ttacattctg catctaaatg taaatgttag ctcttgatta tacgcaaatt
       61 aatgaactta gatctcaatt ccctttgtaa tatgggatag attatttagg tttatctgat
      121 atttttttgg agctattaat atgcatgctt attcctttca attatatagt cttgccttgt
      181 tggcaaccat tatcattgcg acagttactg ctatagtact ctggccgcgt cgcaaattca
      241 aaggtgtact atggatgatc ttacttgaaa tcgccgcagc ggagtgggca ttcggtattt
      301 tattcgaatc agccgccact actcaccgat tgaaagaatt atggtcggca attgcctata
      361 ttggtacttg ttctgttcca ctcttctttt ttctcttcac ggttgaatat taccgaggac
      421 gattacagat ttctaaaatc gcactaacgt ttctcataac cattccaatt ttcatcatta
      481 ttttggcatt cactaatcct ttgcacaatt tgctctggcc caatattacc atcaacccgg
      541 caactaatat agcgatctat tctcatggaa tcgtgtggtg gattacttac gtttatgaat
      601 acgctcttat cattattgga atgtatttcc tactaaagat ggcgtttgaa tccggctctt
      661 tctatacgcc tcataatctg acgataatta taggtgcttt aataccaata atcggcaacc
      721 tgatatatgt tttcggtccg aatcctgtgc caggattaga ttgggctcca gttggattcg
      781 ctttatcggg aattgtaatc acctgggcaa ttctgagatt acaattattt aaacttagac
      841 caatcgctta ttcattttta gtagataaaa tgctcgatgg aatgattatt ctggacacta
      901 ataatgtaat cgtagatata aatccagcgt ctgccaatat ctttcataaa cgacccaagc
      961 aaatgattgg ccaaaaaata aatcgttttc tccctcaatc aattgatcta aagaatttgt
     1021 tacttgccga aaatcctagc cagatcgaaa tagccctggg taaaatccaa ccggatcgca
     1081 tatttgaact acggctctct attttgcgag ataactattc ccagataatt ggtaaaagtc
     1141 tccttttacg cgacatcacc gaaaaaaaga aaaacgaaaa tgagcgtgaa aaattaatcc
     1201 gtgacctaca aaatgctatg agccaaatta aaactctcag cggtcttttg ccaatctgtg
     1261 cgcattgtaa aaaaatacgc gacgataaag gttattggca cgccgttgag gaatacattc
     1321 atgaacattc cgatgctgat ttttcccata gtatttgtcc ggattgtctc gaaaaatatt
     1381 atcctgaaat tgcgaaaaaa atgaaatcaa gccagagtta aatatccagc taacgtctca
     1441 ttaaattacc ttaaaaatta attatcaata ctgctaataa ttgaaaggat aacgaaaatg
     1501 aaagccgaag ggaaaaattc agaacaagat caatatcggt ttcgaatatt aaaaacggcc
     1561 gatctgggta attatcagac cggagtaatc gttagccgga ccttgattga cagaccggct
     1621 ggaaacgtga ctttcttcgc cttcgatgaa ggtcagcgac tgagcgaaca cactgcaccg
     1681 ttcgatgcac tggtgaacat tctcgacggc gaagccgaga tcacgattgc cggtcaatca
     1741 ttcagcgtca aagccgacga aatgattatt atgcccgcca atcaaccgca cgccgtaaaa
     1801 gccgtcaaac ggtttaagat gttacttacg atgattaaag gataatatcc actatttata
     1861 atctgcgaat ctatcgcgat tattatccaa atcagaataa aaataatttc cctcattaac
     1921 tggtggtgtt atttttttcc caatattacg acggagattt acatttatca ttcaacaacg
     1981 cagaccaatc ccttgagata atgaccttcg gggaagttta aagccgtcgg gtgatcggcc
     2041 gactgattga gccagaccaa gatttgggct gattttccag catcaagggc agaatcagct
     2101 acgattttct gaaataattc cggcgatatc tggcctgaac aagagaaggt aaaaagtata
     2161 ccgcccggcc gtaaaagctt aaacgccagc aggttgatgt ccttatagcc gcgactggca
     2221 cgttcaattt gacttttcga ctcggcaaat ttgggaggat cgaggataat taaatcgaat
     2281 gaccgtcctt gatcacgaaa ctttcgcaga acttgaaaaa catcatcggt cagattttcg
     2341 actcgggccg gagcgaaacc attcagggct atgttgcggt tcagtaattc cagcgcatcg
     2401 gcggaagttt cgatgtttgt tactttgatg gcgccggctg acaatgccgc aaccgcgaat
     2461 ccgccggtat aagcgaaaca gtttagtact tcgcgttcgg ctgaaaattc ggtaattttt
     2521 tgccgattat cgcgctgatc gagataaaaa ccggtcttgt gaccattgcg aatatcaact
     2581 aagaaacgtc ggaaattctc acaaatttcg attaattctg gtggttcgtt tccttgtaat
     2641 aaaccccgac gaagttctaa accttctttt tcgcgcactt ccacatccga gcgctcataa
     2701 attccggtaa ccgggacgat ttcggccagc accgtgacga tcgtctcacg ccagaattca
     2761 gctccggctg aaaggaattg aactaccaga taatcgttat aacgatcaac gatcagtcct
     2821 ggtaaaccgt cggattcggc attgaccaag cggactgcgt cagttttgtt cggctcaaaa
     2881 agcgactcgc ggtgtttgat cgctaaactc aattgacgtc ggaaaaagtc agcgttgatt
     2941 tcatcatccg gattgaaact ccaggtgcgc acagcgattt gagactttgg cgaccaggca
     3001 ccgaacccca ggaaacgacc gtcctgcgcc cgtaactcga cggtctcacc caactgcggt
     3061 tcaccttcaa ttctttcaat cgcacctgaa aagagccacg gatggtgacg caggagcgat
     3121 ttttcacgat ctttctttat taataccttt acactcatac tatttcctta gtcaatagac
     3181 cccagcctgc gtttatctcc gctccgcgcc gtcatccctt tttggtcgaa gtaattcaac
     3241 agtggcaagg caaatttgcg cgatataccg agcacttcct tcaactcgct gacggtagcg
     3301 ccgctttttc cacgtaggaa ttctatcaac tttgtttccg cctcggcgac tgccgccgcg
     3361 ctgaacggat atattctgtc aattaaaact acttcttgtt gactgactaa gaggtttatc
     3421 aattgacgca tttcggcgag atcggtctgt aatttctcag ccagttcggc taaagtaggc
     3481 ggcgtcaaag ctgattttgt gatttcagcc agaatttgtt cctgccgagt agtttgccgc
     3541 tcacttaatt gcggagcaaa accggcaata gctaatttat cgccgatgac cctgattttg
     3601 cccgcctgag tcaaatcctc agtcaaccaa tcgcccagca tttcatcgaa atgcaaattg
     3661 ccgaataacg tggctttttc cagaccggga agaaggggat tctgtcggtg aaattcagct
     3721 aaagttgcgg taattttttc agcgcattca gccaatcgtt cggcggcgat aattttgccc
     3781 tcgcgctctc gaatgacatt ttccttaacc aatttcgcca atagcggtga aatttcagcc
     3841 acagtaacgg tcagaacttg cgccagtttt tcgattgtaa tcagtttttc accctggcgg
     3901 aatattaacc acttcaacca ttcctcattt ccggcgcaaa ccaatcgttc aagttctgcg
     3961 gcaatcggtt catccttttt gcgcagaggt tctggagaat tttccagcac aattccaccg
     4021 ccaatcgtta ccaaaggtga atacgagcgg agaataaaat gatcacgaaa tccagcgcta
     4081 atcggctgat cgagaactat ttgcgcgact ccttgctttc ccgggaagag gcgatcgcga
     4141 ccaatcaatc tgatccgcac aatatactca ccggtgccga gatgcaatcg caactgatca
     4201 ttgtattcaa tcggcggcgc tgacggcaga gattctatat aaaccgtcag caaattcgtt
     4261 ttcacgaaag caccgagcgt tgcgacaatc attccacgtt cgatttgttc tttacttact
     4321 ccaaccagat taagggccgc acgttgaccg cggg
//
LOCUS       DAOSMP010000098         4342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_20269_length_4342_cov_18.394915, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000098 DAOSMP010000000
VERSION     DAOSMP010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20269_length_4342_cov_18.394915"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..1969)
                     /gene="aspS"
                     /locus_tag="PKX09_09735"
     CDS             complement(197..1969)
                     /gene="aspS"
                     /locus_tag="PKX09_09735"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOU18141.1"
                     /translation="MLKRTLTCGDPRVEHVEQIVILNGWVNRRRDHGGLIFVDLRDRY
                     GLTQVVFDPQSGEEIMTLARKLGLEDVIAVRGKIRRRPENAVNREMATGAVELLAIEL
                     EILNEARPQPFLVTDRKTGLEDLRLKYRYLDLRTSELQRNIAIRHKTYQSVRNFYYEH
                     NFYEIETPFLTRSTPEGARDFLVPSRLCQGRFYALPQSPQQYKQILMVSGFDRYFQIV
                     RCFRDEDLRADRQPEFTQIDVEMSFVEEEDVLQSTEEMLIRVFREVAGINLESPFPRI
                     PYDEAMGQYGSDKPDLRFGYEIHTLDKIVERGEFEVFNKALASGGTVAGICFPQGASI
                     TRKLIDELTEQVKKMGVQGLAFTRVTANGLEGGIARFLTPVAREIIAEFDANDGDIIF
                     FSADSREKTYSALGVLRLEIAKRLQLIPANVFKPVWVTRFPLLEWSAEENRLVAMHHP
                     FTAAVPEEAELLATNPAQVHARAYDIVINGHEIGGGSIRNHTVEMQKRMFDVLQINPE
                     TAQEKFGFLLEALSYGAPPHGGIALGFDRLVMVLAGAENLREVIAFPKTTSGASLMDG
                     SPAPVEEQQLKELGLKLIKGKSSF"
     gene            complement(2294..2707)
                     /locus_tag="PKX09_09740"
     CDS             complement(2294..2707)
                     /locus_tag="PKX09_09740"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOU18142.1"
                     /translation="MSAIIKKKESSINTIIGEKTIITGSLRLEGDIIIYGKISGDIET
                     DGTITIFPGAEIKGNLQSANLNIGGHVEGDVIATGKVTLGDRSFLRGDIKAAQIVIED
                     GAVFEGHCEMHFPYPLEAQSPNAAKPAADDANLPQ"
     gene            complement(2709..3518)
                     /locus_tag="PKX09_09745"
     CDS             complement(2709..3518)
                     /locus_tag="PKX09_09745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU18143.1"
                     /translation="MKRKYTLHLLSESRAKPIIWTFSHTTARLIIIGSLAFLALLIVT
                     ILITLPKAIKYRQVTLENAQLVQDRLKVSQILSDYNEIRQMDQYIRSVLGTDLVLTPS
                     DSVSGEAVNYLKNDPDQNRLVQISFLENIPIYPPVDGYITRGFIDDKVMTSENHYGVD
                     VAAAEGTPIKAAAAGVVVFSNWTYDLGYTVILYHSDGYFSIYGHNQRNSVVAHQYVKR
                     GEVIGYLGNTGKSQGPHLHFEIWREGVPINPQELIYSYKKADISLKYEGEY"
     gene            complement(3515..>4342)
                     /locus_tag="PKX09_09750"
     CDS             complement(3515..>4342)
                     /locus_tag="PKX09_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOU18144.1"
                     /translation="PEFPEGIEQVYTMAEIEVAQIKFNPHQPRQEFGAQAMAELIMSI
                     REKGVIQPITVRQVDGGFELIAGERRLRACQELGKTTIPAYIMPVATEAEMLELALIE
                     NVQREDLNPVDEARAYQILASTFNLSHEEIARKVGKERPTITNSLRLLNLPDAILADL
                     RSGELSAGHARPLLSIINPQQQINLWQKIKREGLSVREVEKLAKKFQNIPLKEKPLPP
                     VRTPTTKAIENRLIHILGTKVKIKGDENKGQITIEFYSREDLARLLEIFKIIEEKTL"
BASE COUNT         1153 a          990 c          981 g         1218 t
ORIGIN      
        1 actccagaaa ggtttttatt agcatttgaa aaaaatgaat tgatgattga tttacgaatg
       61 catttgaggc catccggagg tgttagaaat catgggactg gattccggat ttcagagaaa
      121 aatcttgttg atttgtatga aaagaaaaaa agattgatct agattttttt acaaaaacat
      181 cgctaaaaaa gtatatttaa aaagaggatt tacccttaat cagttttaag ccgagttctt
      241 tcaactgttg ttcttctacc ggggctgggc taccatccat cagggaagcg ccactggtag
      301 ttttcggaaa agcaatcact tcccgcaaat tttcggcacc tgccaatacc ataaccaggc
      361 gatcaaaacc cagagcaatt ccgccgtgcg gcggcgcgcc gtagctcagt gcctccagta
      421 aaaaaccgaa tttttcctgc gctgtttcgg gattgatctg caagacatcg aacatccgct
      481 tctgcatttc aacagtatga ttgcggatac taccgccgcc gatttcgtgg ccattgatca
      541 caatgtcgta tgcccgtgcg tgtacttgcg ccggattagt cgctaataat tcggcttctt
      601 ccggcacagc ggcagtgaag ggatgatgca tggcgaccag acgattctct tctgcgctcc
      661 attccagtaa ggggaagcgt gtcacccaaa ccggcttgaa aacattcgcc ggaatcaatt
      721 gcaagcgttt ggcaatttcc agccgcagga cgccgagcgc cgaataggtt ttttcccgag
      781 aatcggcact gaaaaaaatt atatcaccgt cattagcatc gaactcagct ataatttcgc
      841 gggcgaccgg cgtcaagaaa cgagcaattc caccttctaa accgtttgcc gttactctgg
      901 taaaagccag tccttgaact cccattttct taacctgctc ggttaattca tcaattagtt
      961 tgcgagtaat cgaagcccct tgagggaaac aaattccggc aactgttcct cccgaagcca
     1021 atgctttgtt aaaaacttcg aattcaccgc gctcgacaat tttatccaaa gtatgaattt
     1081 cataaccgaa acgcaggtcc ggtttatcgc tcccgtattg ccccatagcc tcatcatagg
     1141 gaatgcgggg gaaaggcgat tcaagattaa ttcctgccac ttcgcggaaa acgcggatta
     1201 gcatttcttc ggttgactgt aatacgtcct cttcctccac aaatgacatc tcgacgtcaa
     1261 tctgagtaaa ttccggttgg cggtcggcgc gcaaatcctc atcccggaaa cagcggacaa
     1321 tctgaaaata gcgatcgaag ccagacacca ttagaatctg cttatattgc tgaggcgact
     1381 gcggtagagc atagaaccga ccttgacata ggcgactggg cacgaggaaa tcacgcgctc
     1441 cttcgggagt actacgcgtc aaaaaaggag tttctatctc atagaaatta tgttcataat
     1501 aaaaatttcg cacactttga taagtcttat gccggatggc aatattgcgc tggagctcgc
     1561 tcgtccgtaa atcgagatag cgatatttca agcgcaaatc ttccagccct gttttgcgat
     1621 cggttaccag gaaaggctga gggcgtgctt catttaaaat ttccagctcg atagccagta
     1681 gttcgacggc acccgtagcc atttcgcgat tgacggcatt ttccggacgg cgtctaatct
     1741 tacctcgcac ggcaattaca tcttccaaac cgagtttacg agccaaagtc attatttctt
     1801 cacccgattg gggatcaaat accacttgcg ttagaccata acggtcgcgt aaatcgacaa
     1861 agattaatcc gccatgatcg cgccgacggt tgacccagcc attgagaatc acgatttgtt
     1921 caacgtgttc gacgcggggg tcgccgcagg tcagagtacg ttttaacatt gcttatccat
     1981 tatttaaata atctttcaaa cgggtaaaat taatgattcc atatagtttt aaccagcaga
     2041 aaaatgattt cgactaaacc ggtagctctt aatagtaatc accgaaaaaa tatccagaac
     2101 gtaatcatca gaaaaagctg gttgatgatc atcggataat cccagtcgtc ccacctgata
     2161 ggcattaaat tggcattgat cagaccggaa aatttcatag agtaatttgt ggttcaataa
     2221 ttaacgtttt taatagggaa aaatatacag ttgtcccaaa atagcaaatt ggggtcaatt
     2281 aatgcggtaa tttttattgc ggcagattcg cgtcatccgc tgccggcttt gccgcatttg
     2341 gcgattgggc ttccagggga taagggaaat gcatttcaca gtgtccttcg aaaaccgcgc
     2401 cgtcttcgat cacgatctga gcggctttga tatcgccgcg taaaaaagag cggtcgccga
     2461 gggttacttt tccggtagct atgacatccc cttccacatg cccgccgata ttaaggttag
     2521 cgctttgaag gttgcctttg atttcagctc cggggaaaat tgtgatggtg ccatcggttt
     2581 cgatatcacc gctaattttc ccgtagatga ttatatcgcc ttccagccgt aagctcccgg
     2641 taatgatggt tttctccccg ataatagtat tgatggaaga ttcttttttc ttgattatgg
     2701 cagacatatt aatattctcc ttcatactta agggaaatat cggctttttt ataggaataa
     2761 atcaattcct ggggattgat cggaacacct tcgcgccaga tttcaaaatg caggtgtggt
     2821 ccctgacttt ttccggtgtt gccaagatag ccgattacct cgccgcgttt cacatattga
     2881 tgagcgacta cactattacg ctgattatgg ccatagatgc taaaatatcc atcggaatgg
     2941 tagaggatga cggtatagcc gagatcataa gtccagttag aaaaaaccac taccccggcg
     3001 gcggcggctt tgatgggagt tccttcggcg gcggcaacat ctacaccata gtgattctcg
     3061 ctggtcatta ctttatcatc aatgaacccg cgcgtaatat aaccatccac tggcgggtaa
     3121 atcggaatgt tttctaaaaa actgatctga actaaccggt tttgatcggg atcattcttt
     3181 aggtaattga cggcttcacc ggagacagaa tcgctgggag tcaggacgag atcggtgccc
     3241 aataccgaac gaatatattg gtccatctgg cgaatctcat tgtaatcact cagtatttga
     3301 gatactttga gacgatcctg aaccagttgg gcgttttcaa gagtaacctg ccggtatttg
     3361 attgctttgg gtaaggtaat cagaatagtg actatcaata gagctagaaa agctagcgaa
     3421 ccgataataa tcaaccgggc agtcgtgtga ctaaaagtcc agataatagg ttttgcgcgg
     3481 ctttccgaaa gtaagtgtaa ggtatatttt ctcttcataa tgttttttcc tcgatgattt
     3541 tgaatatttc cagcagacgt gccaggtctt cgcgagaata aaattcaatc gtgatctggc
     3601 ctttgttttc gtctccctta atttttacct tggtaccgag aatgtgaatc aatctattct
     3661 caatggcttt ggtagtaggg gttctgactg gtggcaatgg cttttctttg agagggatat
     3721 tttgaaattt cttagctagt ttttcgactt cccgaacgct caaaccttct cgctttatct
     3781 tttgccagag attaatttgt tgttgtggat ttataatgga gagaagcggt cgggcatggc
     3841 cggcgctgag ttcgccagac cttaaatcag ccagaatggc atccggtagg ttaagtaagc
     3901 gtaaggaatt agtgatcgtc ggtcgttctt tgccaacttt tcgagcgatt tcttcgtggg
     3961 atagattgaa tgtgctggct aaaatttggt aagcgcgggc ttcatcaacc ggattaaggt
     4021 cttcacgctg gacattttca attaaagcca attccagcat ttcagcctca gtggctaccg
     4081 gcataatata agccggtatg gtcgtcttac cgagttcttg acaagctcgc agacgacgct
     4141 caccggcgat taactcgaag ccgccatcga cctggcgcac ggtgatcggc tgaatcactc
     4201 ctttttcccg aattgacatg atcagttcag ccatcgcctg agccccaaat tcctggcgcg
     4261 gctggtgtgg attgaacttg atttgagcca cttcgatttc agccatcgta taaacctgtt
     4321 ctattccttc cggaaattca gg
//
LOCUS       DAOSMP010000099         4255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_20821_length_4255_cov_19.915238, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000099 DAOSMP010000000
VERSION     DAOSMP010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20821_length_4255_cov_19.915238"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..1701
                     /locus_tag="PKX09_09755"
     CDS             310..1701
                     /locus_tag="PKX09_09755"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU18145.1"
                     /translation="MPSLTFLGATGTVTGSRFLLKTAHKQYLIDCGLFQGLKELRLRN
                     WEPFPISPEDIDAVILTHAHIDHTGYLPRLVHYGFKGTIYATTATTDLCQILLPDSAH
                     LQEEDAEYANKKHFSKHSPALPLYTTEDALQALELFTPIAYGSRLQLDDGVSLTFRDA
                     GHILGSSFIDLRLSIDGEQRRLLFSGDLGRPNQPILRDPHTVFGADYLIVESTYGDRL
                     HGNEDPKEVMARIINESIRRGGVLLIPSFAVGRTQELIYTIRELEEANRIPHLPIYMD
                     SPLAIKATKIFSKNKQDFDLKSKTLELKGKNVLETAKLHFAQTQEESKAINKIKSNAI
                     IISASGMATGGRILHHLFNRLPDPKNTVLFIGYQAVGTRGRTILDGAESVKIHGQYVP
                     IRAHIESTSCFSAHADYHEILAWLSNFNSGPRKVFIVHGEPEPARSLSDKINQHFGWS
                     TELPEYLGQYQIS"
     gene            1734..3068
                     /locus_tag="PKX09_09760"
     CDS             1734..3068
                     /locus_tag="PKX09_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOU18146.1"
                     /translation="MDLFEANPKNTNLLPPLAERMRPRRLEDFKGQEEIIAQGKILRK
                     ALESRKIFSMILWGPPGSGKTTLAKIIANYVDADFYQISAVSAGVKDVRSIIDKAAYN
                     RSRNRSTLLFIDEIHRFNKAQQDALLHAIEDGSLILIGATTENPSFEVIAPLISRCRV
                     LKLEGLSDENLASIINTAIAKDIILLKYKIKFEPGAQDFLIKNVNGDARKLLNAIEIC
                     QQIAAPEEGAEFTITIDLIKEALQQRRQLYDKTGDYHYDTISAFIKSVRGSDPDAAVY
                     WMALMLEGGEDPLFIARRLIILASEDIGNADPSALPLATAGFQAVHAIGMPEAAIVLA
                     QVTTYLASTFKSNASYMALENARQALKEGSAPSVPLHLRNAPTKLMKAQGYHKGYRYP
                     HSYPDHFVKENYFPGDGLAPTFYQPTSIGREKFLRERLEQLWPERYRGTKNS"
     gene            3334..3591
                     /locus_tag="PKX09_09765"
     CDS             3334..3591
                     /locus_tag="PKX09_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOU18147.1"
                     /translation="MRRKIVRVGASLCVLLPKEVVRAMGWDFEDEINLILDEENEVVI
                     LRNLKNQPIEKKTMMEHFDQFLENYGKELGTLVADHDPQIQ"
     gene            3606..3848
                     /locus_tag="PKX09_09770"
     CDS             3606..3848
                     /locus_tag="PKX09_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18148.1"
                     /translation="MKRKIIRVGSSKGVLLPREVTKAMDWGFGSEIELSVDEEKHEVY
                     LKSIKVSVPQDYEIDHLRQIEEVMEDYADLLEGIDD"
     gene            3841..4239
                     /locus_tag="PKX09_09775"
     CDS             3841..4239
                     /locus_tag="PKX09_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18149.1"
                     /translation="MTEEQIRQIKLLSYANWLVTEKFGAEKGIRDIKALTAAVTLPGN
                     GTLSCVLGERLSNRKDLYAQLGWFVYYMIEGEPFNSKNRTTVLLILLWVLKYYRLEFN
                     AEELANYIKTLDPLKHGNSDISLWFSTHCR"
BASE COUNT         1261 a          838 c          941 g         1215 t
ORIGIN      
        1 tctcagccag ttgttggtag ttcgaagaaa gtctaaaaag ttttactaaa ataaattgat
       61 aatgtattag ggaaattaac tctctgagag cgcctttagt gaaaagggct ctgataatat
      121 tttacagaag gttttacttt taccgggaga tgtgtttaat aaaagaaagc cgggcttgaa
      181 tacttctgat taaataacca gctacatatt ggaaacctcc tgagagttca atatattctt
      241 tttgatgaac gattattcga ttaatcgctt gaaaaattta aaagttggta gcaacttcag
      301 ggggacaaga tgccaagcct aacatttctc ggcgcgaccg gtacggtaac cggttcacgt
      361 tttttgttaa aaactgccca taagcaatat ctaattgatt gcggtttatt tcaaggttta
      421 aaagaattac gtttacggaa ctgggaaccg tttcctattt cgccggaaga tattgatgct
      481 gtgatcctta cgcatgctca tattgaccat accggttatt tgccacggtt ggtacattat
      541 ggttttaagg ggacaatata tgccacaacg gctacaactg acctttgcca aatattgtta
      601 cctgattcgg ctcatttgca agaagaagat gcggaatatg ccaataaaaa acatttttcc
      661 aaacacagtc cagccttgcc actttatact acggaagatg ctctgcaagc cctggaatta
      721 tttacgccga ttgcttacgg tagtcgttta caattggatg acggagtttc tctaaccttt
      781 cgagatgctg gtcatattct ggggtcttct tttattgatc tgcgtttgtc tattgatggt
      841 gagcaacggc gtttgttatt ttccggtgat ttaggacgac caaatcaacc tattttacgc
      901 gatccgcata cggtttttgg tgccgattat ctaattgtcg agtcaactta cggcgatcgc
      961 ttgcacggta atgaagaccc gaaagaagta atggcgcgca taatcaacga aagtatccga
     1021 cgcggcggag tgttgctgat accatctttt gccgtaggtc ggactcagga attaatttac
     1081 acaatccgtg aattggaaga agctaatcga attccacatt taccaatcta tatggacagc
     1141 cccttagcca tcaaggctac gaaaatattt tctaaaaaca agcaggattt cgatctgaaa
     1201 tctaaaactt tagaattaaa gggcaagaat gtattagaaa cggctaaatt gcactttgcg
     1261 caaacccagg aagagtccaa agccataaat aaaataaaaa gcaatgccat catcatttct
     1321 gccagcggta tggctaccgg cggcagaatc ctgcaccatc ttttcaatcg cctacccgat
     1381 ccgaaaaata ccgttctatt tattggctat caggcagttg gcacgcgtgg tcgcacgatc
     1441 ctcgatggcg ccgaaagtgt caagattcac gggcaatatg tcccgattcg ggcgcatatt
     1501 gaaagtacat cctgtttttc cgctcatgct gattaccacg agattttagc ctggctatcg
     1561 aattttaatt ctggaccacg aaaagttttt attgtccatg gcgaaccgga acctgcccgt
     1621 agtttgagtg acaaaatcaa ccagcatttc ggctggtcaa ccgaattgcc ggaatacctg
     1681 ggtcaatatc agattagcta aaatgtggtc tatttaggca aggaaaaata tcaatggatt
     1741 tgttcgaagc gaatccgaaa aatacaaatt tgttaccgcc cttagcggag cgaatgcgtc
     1801 cccgacgcct tgaggatttt aaaggtcaag aggaaatcat cgcgcagggc aaaattttgc
     1861 ggaaagcttt agaatcccgg aaaatttttt caatgattct atggggtcct ccgggcagtg
     1921 gaaaaactac tttagccaaa attattgcta attatgtgga tgccgatttt taccagatca
     1981 gtgccgtttc agccggagtg aaggatgtgc ggtctattat tgataaagcg gcttataatc
     2041 gcagtcgcaa tcgctcgacc ttattgttta ttgatgaaat tcaccgcttc aacaaagctc
     2101 aacaggatgc tctcctccat gccatcgaag atggatcgtt aattctaatc ggagccacga
     2161 ccgaaaaccc ctcttttgaa gtgattgcgc cgctcatctc gcggtgccgg gtattgaagc
     2221 tggagggatt atctgatgaa aatctggcaa gtattatcaa tacggctatt gctaaagaca
     2281 ttattctatt aaagtataaa attaaatttg agccgggtgc tcaggatttt ttgataaaaa
     2341 atgtcaatgg cgatgctcgc aaattgttga atgcgatcga aatttgccag cagatagcgg
     2401 cgcccgagga aggcgctgaa tttactatca ctattgattt aattaaagaa gcactccaac
     2461 aaagacgaca gttatacgat aaaactggtg attatcacta tgatactatt tcggctttca
     2521 tcaaaagcgt gcgcggcagt gatccggatg ccgccgttta ctggatggct ttgatgttag
     2581 aaggtggtga ggacccgcta ttcatcgctc gccgtttgat catactggca agtgaagata
     2641 taggcaatgc cgatccgtcg gctttgccgt tagcgaccgc tggttttcag gctgtgcatg
     2701 ctatcggtat gcccgaagcc gctattgttc ttgcccaggt cacgacctat ctggcatcca
     2761 cgttcaaaag taacgcttcc tacatggcat tagaaaatgc ccgccaggca ctcaaggaag
     2821 gcagtgcgcc cagcgttccc ctgcatttgc gcaatgcgcc aaccaaatta atgaaagccc
     2881 aaggttatca taagggatat cgttatcccc atagttatcc agaccatttt gtgaaagaga
     2941 attatttccc cggtgatgga ctggcgccga cgttttatca accgaccagt atcggtcgtg
     3001 aaaaattcct acgcgagcgc cttgagcagc tctggcctga gcgttaccga gggacgaaaa
     3061 attcctgaaa taattttatt tatctataca atttttcgct tgggattatt aaataattat
     3121 ctgtgaatat cagatatttg gatctaaaaa taataattta tatctctgat gaacataaca
     3181 accattggga tatttatttt gacgttaaat aggctattta ataccagatg aagtatacca
     3241 ataagtatat ttatattata aaaaaagctt gattatttct aatagttaat ttattttgag
     3301 ttgaaattta caaaacgcta taggggcgat gtaatgagaa gaaaaatagt tagagttggt
     3361 gccagcctat gtgtgctatt acccaaagag gtagttagag ctatgggatg ggatttcgaa
     3421 gatgaaatca atttgatcct agacgaggaa aatgaagtgg taattctccg caacctgaaa
     3481 aatcagccaa ttgagaaaaa aacgatgatg gagcatttcg accagtttct cgagaattac
     3541 ggaaaggagc tgggaactct ggttgccgat cacgaccctc aaatccaata attaggagaa
     3601 aaaagatgaa aaggaaaata attcgggtgg gttccagtaa aggagtctta ctacctcgcg
     3661 aggtgactaa ggcgatggat tggggttttg gttccgaaat cgagcttagt gttgatgaag
     3721 agaagcacga ggtttattta aagagtataa aagtcagcgt accgcaagat tatgagatag
     3781 accacctgcg ccagatcgaa gaagtaatgg aagattatgc tgatttgttg gagggtatcg
     3841 atgactgaag aacaaatcag gcaaattaag ttgttatctt atgctaactg gttagtaacc
     3901 gagaaatttg gcgctgaaaa aggcatccga gacattaagg ctctaactgc ggctgtaacc
     3961 ttaccgggga atggaaccct ttcctgcgtt ctgggcgaac gtctatccaa tcgcaaggat
     4021 ttgtatgctc aactcggatg gtttgtttat tatatgattg aaggtgaacc gtttaatagc
     4081 aagaatcgca caacggtgct tctaatttta ttatgggtct taaaatatta ccgcctcgaa
     4141 ttcaatgccg aagaactggc taactatatt aaaacgctcg atcctctgaa gcatggtaat
     4201 tccgacattt cactctggtt ctcgactcat tgtcgttaaa acacttactt ttttc
//
LOCUS       DAOSMP010000100         4152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_21540_length_4152_cov_5.086649, whole genome shotgun sequence.
ACCESSION   DAOSMP010000100 DAOSMP010000000
VERSION     DAOSMP010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21540_length_4152_cov_5.086649"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..609)
                     /locus_tag="PKX09_09780"
     CDS             complement(214..609)
                     /locus_tag="PKX09_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOU18150.1"
                     /translation="MTEAYKYSDITGKIIGCAMEVHKFLGNGFQEVIYQRALAIELER
                     VGLNFQREIEQPIYYKDVNHPIGERRVDFIVENCVLVELKAVIQLEEVHWAQILNYLK
                     AYRFEVGLLMNFGAKSLEWKRFVSSGKNV"
     gene            complement(719..>4152)
                     /locus_tag="PKX09_09785"
     CDS             complement(719..>4152)
                     /locus_tag="PKX09_09785"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HOU18151.1"
                     /translation="FSDWREAGLLYQVTGEDLSNQGFLFEIKFDIDRMQSYLFYAIEL
                     EPKIYQRGKLAQITREVNKLFPMPVFILFKYGNRLTFSIIDRRQHKRDESKDVLEKVT
                     LIKDISIANPHRAHIEILYDLSYPELLTNFRFSNFEGLHKAWQKTLDIDVLNNKFYKE
                     LYHWYLWASKMVKFPQIRAIEDMIDDKIHQSESLIRLLTRLLFCWFMKEKGLIQDELF
                     RLSYLQTILKNFNGINSEDTIYYKAILQNLFFATLNKPVKDRELLRKEFPNPHYGDQL
                     VYRYEELFNPDVDILKLFSEIPFLNGGLFDCLDQRKDENNPVEIRLDGFSSKPLKQPT
                     VPDKLFFGEYKGIDLSNEYDDKKKSNVTIHGLIDIFNKYKFTIEENTPVEEEIALDPE
                     LLGKVFENLLASYNPETKISARKQTGSFFTPRAIVNYMVDESLVAYLDQQMKEHLSEL
                     KEMDSLNEVIREVLSYTEKSHPFSDKEITVLIQALDNIKILDPACGSGAFLMGMVHKM
                     LHLLRKIDPENKLWFEKVIAKFPVIMQNEMRKKLEPENWDYIRKLGLIQECIYGIDIQ
                     PIAIQIAKLRFFISLLVDQKEKPGMENRGLEPLPNLDFKLVCANTLISPPESEAIKVG
                     LFSAAKDDFSVQFERLTEQYFSAYLPGEKKKPKDDIANLINRKCQEKIGEIEKKTESD
                     DEKTRQMLKERHKNVIGQKNREIALWNSYKNLFKHEAVGFFEAKYFFPKVTGGFDIVI
                     ANPPYVRADSPDLKAQRDAIMQSGRYETLYEKWDLFIPFIEQGLKLLSRSGFLSYITS
                     NSLLTSKFAFRLLEYIQHNYDAQFIDYFTDDTKVFEAGVVPVVFGLSRQAVSQSVRKT
                     IHHGKFNDVAQKEIIPLIDFKSLGRDAFRLSSNSFKPTIQTILLGDICYLSYGLRPNS
                     DERYWQGEFTRDDLVSETKDATHPVPYVEGKNIGHYRIDKVLYLEWGTDRVPKKLVRP
                     TFPELYDRPKILCGIMTGGTFDNAHIICNHSIVVLVRFAELKNIDNKSISGSLKKFNS
                     IPRAELEAISTRFDLKYILAILNSKFANRFLNSIRRHRLENYFYPDDFRKLPIADISV
                     KNQKPFIEIVDRILAAKRANPQADTFALEREIDQLVYELYGLTEEEIAVVEGKNV"
BASE COUNT         1180 a          898 c          761 g         1313 t
ORIGIN      
        1 aatctgcgca atctgcttaa tcagcgattc agacattttt cccctccacc accgcaatct
       61 cctcctccgt caattcgtac attgtcagca tcccagatta cactgattaa tggatttcac
      121 agattcactt ccccggtttc attctctata atcagtgcaa tcagcgtaat ctgcttaatc
      181 agcgattcag actttttctg cttaatcagc ggttcagaca tttttccccg aactcacgaa
      241 ccgtttccat tccagactct ttgctccaaa attcatcagc aagcccactt caaaccgata
      301 tgctttcaga taattcaata tctgtgccca atgcacttct tccaattgta taaccgcctt
      361 caattcaacc aaaacgcaat tttccacgat aaaatctacc cgtcgttccc caataggatg
      421 attgacatcc ttgtagtata taggctgttc tatctcacgc tgaaaattta atcctacccg
      481 ctccaattca atagctaacg ccctttggta tatcacttcc tgaaatccat tcccaagaaa
      541 tttatgcact tccatcgcgc aaccgataat cttgccggta atatctgaat atttgtacgc
      601 ttcagtcata acattgtcag catcacagat taaactgatt taaggattcc acagatttct
      661 tttccctgta ttgttctcta gtatcagcgt catcagcgta atccgcttaa tcagcggttc
      721 agacattttt cccttccacc accgctatct cctcctccgt caatccgtac aactcataca
      781 ctaactggtc aatctcccgc tccaacgcga acgtatccgc ctgcggattg gcacgcttgg
      841 cggcaagaat acgatctaca atttcaataa atggtttttg gtttttaact gaaatgtcag
      901 caatcgggag cttacgaaaa tcatccgggt aaaagtaatt ttctaggcga tgacgtctaa
      961 tactatttag gaaccggttt gcgaatttag aatttaggat agcgagaata tattttagat
     1021 caaaacgagt tgatattgct tcaagttctg cgcgaggtat tgaattaaat ttcttgagtg
     1081 agcctgagat acttttatta tctatgtttt tcaattcagc aaaacggacc aacacaacaa
     1141 tactgtgatt acaaatgata tgagcattat cgaaagtacc tcctgtcatt attccacata
     1201 agattttcgg acggtcataa agctcaggga aagtaggacg caccaacttt ttaggaacgc
     1261 ggtctgtgcc ccactcaaga tataaaacct tatcaatccg ataatgacca atattttttc
     1321 cctcaacata aggcacagga tgagttgcgt cttttgtttc tgaaaccaaa tcgtctcgtg
     1381 taaattcgcc ttgccaatat cgttcatcgc tattaggcct taatccgtaa cttaaatagc
     1441 aaatatcgcc caataaaata gtttgaattg ttggcttaaa tgagttagag gatagcctaa
     1501 atgcatctct gccaagcgac ttgaaatcta tgagcgggat tatttctttt tgggctacat
     1561 cattaaattt accatgatga atagtttttc ggacagattg agatactgcc tgtctggaca
     1621 atccaaacac gacgggcaca acacccgcct cgaaaacttt agtatcatca gtaaagtaat
     1681 caataaattg ggcgtcgtag ttatgttgaa tgtattccaa gaggcgaaag gcaaacttgg
     1741 aagttaataa agaattggaa gtgatataac tgagaaagcc cgatctgctt aggagtttta
     1801 gcccttgttc gataaacggg ataaataaat cccatttttc atatagtgtt tcatatctcc
     1861 cgctttgcat aattgcatca cgctgggctt ttaaatctgg actatcagcg cgcacataag
     1921 gcggattggc aatcacaata tcaaaaccac cggttacttt tgggaagaaa tatttcgctt
     1981 caaaaaagcc gactgcttca tgtttaaaca ggtttttata actattccac aaggcgattt
     2041 cacgattttt ttgtcctatg acattcttat gtctttcttt aagcatctgc cgggtttttt
     2101 catcatcgct ctcggttttt ttctcaatct ctccgatttt ttcctgacat tttcggttaa
     2161 taaggttggc tatatcatcc tttggtttct ttttttctcc aggtaagtat gccgaaaaat
     2221 actgttcggt aagacgttca aattgcaccg aaaaatcatc tttagctgcg gagaataatc
     2281 ccaccttaat tgcttcggat tccggcggag aaatcaaggt attggcgcag acgagcttga
     2341 agtcaagatt tgggagtggc tccagtcccc ggttttccat tccgggtttt tctttctgat
     2401 cgaccaatag tgaaataaag aagcgtaatt ttgctatctg tatggctatg ggctgtatgt
     2461 cgattccgta tatgcactcc tggataagac caagtttgcg aatataatcc cagttctccg
     2521 gttccagctt tttccgcatt tcgttttgca taataaccgg gaattttgcg atgacctttt
     2581 caaaccagag tttattctcg ggatcgatct tacgtagaag atgtaacatt ttatgaacca
     2641 ttcccatcag gaaggcgccg gagccgcagg ctggatcgag aattttgata ttgtcgagcg
     2701 cttgaatcag aaccgtgatt tccttgtcgc taaaaggatg gcttttttcg gtataggaaa
     2761 gcacttcgcg aataacttca tttaaagaat ccatctcttt tagttcggaa agatgctctt
     2821 tcatttgttg gtcgaggtaa gcaacaagcg attcatccac catatagttc acaatcgcgc
     2881 ggggtgtaaa aaatgatccg gtctgtttgc gcgctgatat ttttgtttcg ggattgtaac
     2941 tggcaagcag gttttcaaaa actttgccta agagttccgg gtcaagagcg atttcttctt
     3001 cgaccggcgt attttcttca atggtaaact tatacttgtt gaatatatca atcaatccat
     3061 ggatagtaac attcgatttc tttttatcgt catattcatt gcttaagtca atacctttat
     3121 attctccaaa aaacaattta tcgggaactg tcggttgttt tagcggtttg cttgagaatc
     3181 catccaacct gatttcaacc ggattgtttt catctttgcg ttgatcgaga caatcaaata
     3241 aaccaccatt caggaacggt atctcagaaa aaagttttag aatatcgaca tccggattaa
     3301 ataactcctc gtatcggtaa accaattggt cgccgtagtg gggattggga aattctttac
     3361 gaagtaattc acgatcttta accggtttgt tcaaagtagc aaaaaacaaa ttttgaagta
     3421 ttgccttata ataaatcgta tcttcggaat taattccatt gaaattcttt aatattgtct
     3481 gaagatatga aagcctgaaa agttcatcct ggatcagacc cttctctttc ataaaccagc
     3541 aaaacaaaag gcgggtaagc aatcggataa gcgactcact ctgatggatt ttatcatcaa
     3601 tcatatcctc tattgcccta atttgaggaa atttgaccat ctttgatgcc cataaatacc
     3661 agtgatatag ttctttgtag aacttattat tgagcacatc aatatcgagt gttttttgcc
     3721 aggctttgtg cagtccttca aaattactaa acctgaaatt cgtcagtaat tccgggtaag
     3781 atagatcgta gagaatctcg atatgcgccc ggtgcggatt ggcgatgctg atatctttaa
     3841 tcagagttac tttttccaga acatccttgg attcgtcgcg cttatgttgt cggcggtcaa
     3901 taatcgagaa agtcagacga tttccatact taaacagaat gaaaacgggc atcggaaaga
     3961 gtttattcac ttcacgggta atttgtgcca atttcccacg ctgataaatt ttaggctcaa
     4021 gttctattgc ataaaacaga taggattgca tccgatcaat atcaaacttg atctcgaata
     4081 aaaatccctg attagacagg tcttcgcctg tgacctgata gagcaaaccg gcttcccgcc
     4141 agtcgctaaa ta
//
LOCUS       DAOSMP010000101         4005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_22626_length_4005_cov_8.627089, whole genome shotgun sequence.
ACCESSION   DAOSMP010000101 DAOSMP010000000
VERSION     DAOSMP010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22626_length_4005_cov_8.627089"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PKX09_09790"
     CDS             <1..320
                     /locus_tag="PKX09_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU18152.1"
                     /translation="NAEIFTDEKNGILSDFLLMPNYPNPFNSNTIIPFYLKNTNLVQL
                     TIYNLKGELIAKLTDDYFTSGYHSITWNGRDQSGRLSPAGVYFAEIRVRGQVHVRKMM
                     YLK"
     gene            complement(357..1340)
                     /locus_tag="PKX09_09795"
     CDS             complement(357..1340)
                     /locus_tag="PKX09_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU18153.1"
                     /translation="MKVLFIGGTGIISSACSQLAIERGIELYLLNRGQCFRPIPKGAH
                     HLLADIRVRESVKAAIGKMNFDSVVEWLAYTPDQIESDLEVFRGCTGQYVFISSASAY
                     QKPPQKLPITEETPLENPFWEYSRNKIACEKRLQEAFQEEQFPITIVRPSHTYDCTSL
                     PMEGRYTVVNRMRQGKKVIVHGDGSSLWVLTHHRDFARGFLGLLGNPKTIGESFHITS
                     DELLTWNQIFKIIAEAAGTQAQIVHVPSEVIARYDAEWGDSLLGDKMHSVIFDNSKIK
                     RFVPDFKATIPFKQGAKEIIEWYDADPTRKIIDANIDLLIDKLIRRFELIR"
     gene            complement(1352..3445)
                     /locus_tag="PKX09_09800"
     CDS             complement(1352..3445)
                     /locus_tag="PKX09_09800"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOU18154.1"
                     /translation="MKKILRRFFVICPKTGKVRGIKPLRGWSKLLFPITGLLALIWVL
                     IRVIPKPTRATYPCMRVAMPLASGFIVYLIGVIASAAAFMKAKKMLAKSRYLLATGFV
                     VLGLLASLLIFHNSRETAYAKYATIKKEANLPMGMAKGIFPGRVVWVHNPDATNENCV
                     PDSTVSSREGKYTTYRTGLTWFLPQNNNQAVVDSMVSSAIQSLTGETSDSAAWVAIFK
                     YHNTTRGKGAVDYSPGEKIFIKINATSGWGGNYSTTDLSCAKNQNYGISETSPAVVMT
                     VLRHLVNVVGVKQRDIYIGDPMKHIYKHLYDYWHSEFDSVHYLDHDANYSSLGREQAV
                     ASQTEVIFYSDSGKVLRENVWDASRPGEGPVYNDKVYAILEDAEYLINIPMLKGHKRA
                     GVTMFAKNHFGSHTRADAGHLHNGLVAPREMEFEVTRPGYGLYRVQVDIMSHSILGDK
                     NLFYLMDALWATDYELDKPLKWQMAPFNDDFTSSIFASFDPVAIESVGYDFLRSEFTA
                     ERVPAAGTYVQMDGVDDYLHQAADSANWPEGIVYDPDNDGVLFASLGVHEHWNNATEM
                     QYSRNLDPESGTGIELVTITRSVSDAIEAEPANLISALSLYPNYPNPFNATTTIRYAL
                     KAPAHVELKVYNLMGQLVSVLENNYKTAGEYVAVWNGETITGQKAPSGVYVYQLNMAV
                     GKQLAVASRKMLLLK"
     gene            complement(3522..>4005)
                     /locus_tag="PKX09_09805"
     CDS             complement(3522..>4005)
                     /locus_tag="PKX09_09805"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N,N'-diacetylchitobiose phosphorylase"
                     /protein_id="HOU18155.1"
                     /translation="FSHTQGWAIIAETMLGHGDRAYQYLRAYLPAAYNTKAEIRQIEP
                     YVYCQFTNSRYSPREGASRLPWLTGAAAWSYFAATQYILGIQPDYFGLRIDPCLPSNW
                     QEIRIARRFRNKMFDILIKNPNGAQKGICKLTVNNQDLAGNLIPLDIMQNENKIVVEM
                     "
BASE COUNT         1128 a          940 c          822 g         1115 t
ORIGIN      
        1 caaatgccga aatatttact gatgaaaaaa atggtatttt atctgatttt ttactaatgc
       61 caaattaccc taatccattc aatagcaaca cgattatccc tttttacctt aagaacacaa
      121 atctcgtcca gcttacgatt tataatctca aaggcgagct aatcgccaaa ctgaccgacg
      181 attatttcac ctctggttat cattcgatta cttggaatgg acgcgaccaa tccgggcgat
      241 tatcacccgc tggtgtctat ttcgcagaaa ttagggtaag aggacaagtt catgttcgta
      301 aaatgatgta cttgaaatag attttgattt attattaaat caggcatgtc agtattctat
      361 cttatcaatt caaaccggcg tattaactta tcaatcaata gatcgatatt agcatcgata
      421 atttttcggg ttggatcggc atcataccat tctataattt cctttgcgcc ttgcttaaaa
      481 ggaatggttg ccttgaagtc cggcacaaag cgtttgatct tggaattatc gaaaatgaca
      541 ctgtgcattt tatcgcccag caggctatcg ccccattcgg catcatatct ggcaatgacc
      601 tccgacggca catgaacaat ttgcgcctgc gttccagccg cttcggcaat tatcttaaaa
      661 atttgattcc aggtcagcaa ttcatcggag gtaatatgaa aactctcgcc gatggttttt
      721 ggatttccca ataaaccgag aaatcctctg gcaaaatcgc ggtggtgtgt caaaacccag
      781 agcgaactac catcgccgtg aacgatgact ttcttcccct gacgcatacg attgacaacg
      841 gtgtaccttc cttccatcgg taaacttgtg caatcgtaag tgtgtgaagg tctgacaatt
      901 gtaattggga attgctcttc ttgaaaagcc tcttgcaggc gcttttcgca ggcaatttta
      961 ttacgtgaat attcccaaaa aggattttcg agtggagttt cttccgtgat tggcaatttc
     1021 tggggcggct tctgataagc cgaagcggaa ctgataaaaa catattgccc ggtgcatcct
     1081 ctaaatactt ccaaatcgga ttcgatttga tccggcgtat aggctaacca ctccacaact
     1141 gaatcgaaat tcatcttacc aatggcggct tttaccgatt ctctaacgcg aatatcagcc
     1201 agaagatgat gcgcaccttt agggattggc cgaaaacatt ggccacgatt gagtaaatat
     1261 agctcgattc cccgttctat tgccaactga gaacaggcag aactgataat gccagttccg
     1321 ccgataaaca aaactttcat agtaattaac tctatttaag taacagcatt ttacgcgatg
     1381 ctacggctaa ttgcttacct acggccatat tcagctgata gacgtaaaca cccgaaggtg
     1441 ctttttgacc ggtaatcgtt tctccattcc agactgccac atactccccg gcagttttat
     1501 aattattctc cagaacggaa accaattgcc ccatcaaatt atatactttt aattcaacat
     1561 gcgccggcgc ttttagagca tagcggatag tagtggtagc attgaaggga ttcggataat
     1621 tcggataaag gctcaaagcg ctgatgagat tagccggttc agcctcgatc gcatccgaaa
     1681 cgctccgcgt aatggtcact aattcaattc cggtaccgga ttcaggatcg agattacggg
     1741 agtattgcat ttcagtggca ttattccaat gttcgtgaac gcctagactg gcaaataaaa
     1801 ctccgtcgtt gtccgggtca tagacgatgc cttccggcca attcgcagaa tcagccgctt
     1861 gatgcaagta atcatccacg ccgtccatct ggacataagt gccagcggca ggaacccgct
     1921 cagcggtgaa ttccgaacgc agaaaatcat atccgacaga ctcgatcgcc actggatcaa
     1981 aagaggcaaa aatagaggag gtaaagtcat cgttgaacgg cgccatctgc catttgagcg
     2041 gtttatcgag ctcgtaatcg gttgcccata aagcatccat taaatagaat agattcttgt
     2101 cacccagaat cgaatgactc ataatgtcaa cctgaacgcg atacagacca tatcccggac
     2161 gggtaacttc aaattccatt tcgcgcggtg ctaccaatcc attatgtaaa tgaccggcat
     2221 cagctctggt atgtgaaccg aaatgattct tggcaaacat cgtcacgcca gcacgtttat
     2281 gccctttcag cataggaatg ttgatcaaat actccgcgtc ttcaagtata gcatacactt
     2341 tatcgttata aaccgggccc tctcccggac ggctggcatc ccagacattc tctcgtaaaa
     2401 cttttccact atctgagtag aatattactt cagtctgact ggctaccgcc tgttcacgcc
     2461 ctaaactgga atagttagca tcgtgatcca gataatgaac actgtcaaat tcgctatgcc
     2521 agtaatcgta aagatgttta tagatgtgtt tcatcggatc accgatataa atatcacgct
     2581 gtttaactcc gacgacgttt accagatggc gtaaaaccgt cattactacc gccggcgaag
     2641 tttcggaaat tccgtagttc tggtttttgg cacaggacaa atcagtagta ctgtagttac
     2701 caccccagcc actggtggca tttattttga tgaaaatttt ctcacctggc gaataatcaa
     2761 ctgctccttt tccccgagtt gtattgtgat atttgaatat cgccacccag gcggcactat
     2821 cgctggtctc gccggtcaaa gactggattg ccgaagaaac catactgtca actaccgcct
     2881 gattattatt ctggggcaaa aaccaggtta gtccggtacg ataagtagta tatttacctt
     2941 cccgcgaact aacggtcgaa tccggcacgc aattttcatt ggtggcatcg ggattatgca
     3001 cccagacaac ccggccaggg aaaatgcctt tagccattcc catcggtaaa tttgcttcct
     3061 ttttaatggt tgcatatttg gcgtaggctg tttctcggga attatgaaag atgagcaagg
     3121 aagccaataa tcccagaaca acgaatccag ttgctaggag gtagcgcgat ttggctaaca
     3181 tttttttggc tttcataaaa gcggccgcag aagcgattac cccaattaaa taaacgatga
     3241 atcccgaagc cagcggcata gccacgcgca tacagggata agtcgcacga gtcggcttgg
     3301 gaatcacgcg aattaatacc cagattaatg ccagcaaacc tgttatcgga aataataact
     3361 ttgaccaacc acgaagtggt ttaattcctc tcactttgcc ggttttcgga caaattacaa
     3421 agaatcgtcg caaaattttc ttcatcaatt tctctctttc agctttttaa tgattgtcaa
     3481 ataatttctt tctctatttg ttcagtaagg taaaattatt atcacatttc tacaacaatt
     3541 ttattctcgt tctgcataat atccagcgga attagatttc cagccaaatc ttgattattt
     3601 actgtcagtt tgcaaatgcc tttttgagcg ccgttcgggt tttttattaa aatatcgaac
     3661 attttattcc gaaaacgcct cgcaatccgg atctcttgcc agtttgaagg gaggcacgga
     3721 tcaattcgca gaccgaaata atccggctga ataccgagaa tatactgagt agcggcaaaa
     3781 tatgaccagg cggcggcacc ggtgagccac ggcagacgcg aagcaccctc gcgcggactg
     3841 tagcgactat tcgtaaactg acaataaaca taaggttcaa tttgtcggat ttcggccttg
     3901 gtgttataag ccgccggtag ataagcccgt aaatactggt aagctcgatc gccgtgtccc
     3961 aacatagttt cagcgattat cgcccaacct tgagtatgcg agaaa
//
LOCUS       DAOSMP010000102         3987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_22757_length_3987_cov_13.208800, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000102 DAOSMP010000000
VERSION     DAOSMP010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22757_length_3987_cov_13.208800"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="PKX09_09810"
     CDS             <1..1188
                     /locus_tag="PKX09_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU18156.1"
                     /translation="ANKDSYVNAIEWMPGERIIIKPIWEEQAEAGTVSGTAAIEPFTG
                     EAWYTLQGGTSVEWTFNSTYAGHIDMKMKARADGQNIGFDFILNGKNIVDAARKWGQF
                     VVWTGDGDPVTFWTGKSTSEFYEASYAFADMHSFGSLDEIGVVAGENTLKLQYSWNPI
                     SFQWIELYEAGTTNLIARLEPSAAVNNGATPGGIGDWVPQGFNSVALGTNGTVTFNLP
                     DLDAGRYQVRVFYQNAGAAQTGKVLVDGTEATTFSFASQPAGTGLDVITGLFDLTAGT
                     HTLAISGSGVKVDWIQLLNESRISGIKKAPVPDGFALSQNYPNPFNPTTMIRFELGKP
                     SNVKLTVYNLLGQRVATLVNGHMSQGAHIIQFDASHLASGIYFYAIQAGEFKEVKRML
                     LLK"
     gene            1289..2392
                     /locus_tag="PKX09_09815"
     CDS             1289..2392
                     /locus_tag="PKX09_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOU18157.1"
                     /translation="MNRREFNKMLLCAVGSLALPKIDLFGSKLEQAPPLHFPRYRGFN
                     LLAKFSNWGPRSKFEEEDFEIMAELGFDFARIPMSYWCWSSKDDWYRVDEDVLKDIDE
                     VIEFGKLYNIHINLNLHRIPGYCINGRDQEPMDLYEDTPERMQKALDAAIFHWKMFAK
                     RYKGIPNSRLSFDLINEPPHTKEETRHVEVVTALVNGIREEDPHRLIVADGKDVGRTP
                     IFGIADLGVVQSTRGYDPMSVSHYKANWVPKESYQTFKKPTWPLKGDDGTIWDKAMLK
                     SQLIDPWMPLVAKGVPVHVGEWGCYNQTPHDVALRWMRDLLSLWQEVGWGYALWNLKG
                     DFGILDSKRKDVKYEQYKGHDLDRKMLELLKEF"
     gene            2609..>3987
                     /locus_tag="PKX09_09820"
     CDS             2609..>3987
                     /locus_tag="PKX09_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18158.1"
                     /translation="MSPLYKIMLLFIVSLAIAQPSRAKQNYKNFKLAIYCPARDVQRM
                     ADLNWLEKNFNLLDKYLDIDKVYLETYRGQTLVDKETISKAKNFFKERGIQTSGGITY
                     VWNDDQQFLSFCITNPDQRKRMQEVAEYTASMFDEIMLDDFWFLNTKTESDIQAKGDQ
                     SWTDFRLEQAAEASQKLIINPAKKVNPKVKIIIKYPNWYDHFQHCGFNLEKQPAQFDL
                     IYTGTETRDPVYTHQHLQAYESYAIMRYFENIAPGRNGGGWVDPFNRRTLDRYAEQIN
                     MTLFAKARELTLFCFGALLEAIPQEDGSFRPLSPVAPVAGYNLDKIDCFLGKLGQPFG
                     IKTYKPYHSSGEDFLPNYIGMLGIPMEITPDFPVNDEVIFLTEQASFDPKIIEKIKNQ
                     LIKGKDVVITSGLYRALQGKGIEDIVELEYTDKKALVHRFYDWQNVFIAENDILIPQI
                     RYATNDSWEL"
BASE COUNT         1153 a          774 c          946 g         1114 t
ORIGIN      
        1 gcgaataaag attcctacgt aaatgctatc gaatggatgc cgggtgaaag aatcataata
       61 aaacctatat gggaagaaca agccgaagcc gggactgtaa gtggaactgc tgccattgaa
      121 ccttttaccg gtgaggcgtg gtacacctta caaggtggca cgagcgtcga atggaccttc
      181 aattctactt atgccggtca tattgatatg aagatgaaag ccagagccga tgggcaaaac
      241 attggttttg actttattct caatggcaaa aatatcgttg atgccgctcg caagtggggt
      301 caatttgtgg tctggactgg agacggtgat ccggtgactt tttggaccgg taaaagcacc
      361 agtgaatttt atgaagcatc ttacgctttt gctgatatgc atagttttgg cagtcttgac
      421 gagatcggcg tagtagccgg tgaaaacacc ctgaagctac aatattcctg gaatccaatt
      481 tcctttcagt ggattgagct atatgaagcc gggactacaa acttgattgc aagattagaa
      541 ccttcagccg cggttaataa tggtgctact cccggtggta ttggcgattg ggtacctcag
      601 ggctttaact cggtcgcctt aggaacaaat ggaacggtaa cttttaatct gcctgattta
      661 gacgctggtc gctatcaggt gcgggtgttc tatcagaatg ctggagcagc ccaaaccggt
      721 aaagtactgg ttgatggtac ggaagcgacg accttttcat ttgccagtca accggctggt
      781 actgggttag atgttataac tggtcttttt gatttgacag ctggtaccca taccttggct
      841 atttccggta gtggtgtaaa agttgactgg atacaattac ttaatgaatc cagaatttcg
      901 ggtattaaga aagcgcctgt tccggatgga tttgcccttt cgcagaacta tccgaatccg
      961 ttcaacccga ctactatgat tcgctttgag ttgggcaaac cttctaatgt caaattgacg
     1021 gtctataacc tgttaggtca aagagtcgct acattggtca atggtcatat gagccagggt
     1081 gctcatatta ttcagtttga tgccagtcat ttggcgtcgg gcatttattt ctatgcgatt
     1141 caggccggtg aatttaaaga agtaaaacgg atgttgctcc ttaaatagga ctgcgaccga
     1201 ttagtttgaa tggatagagt agccggcagg atgaaaaata tctgccggct ctcttttttc
     1261 agatgcagtt agtgaattag gaaagtttat gaatagacgt gaatttaata aaatgttgct
     1321 ttgcgcagtc ggttcgctgg cattaccgaa gattgacctc ttcggttcta aactcgaaca
     1381 agcaccgcca ctgcattttc cacgctatcg aggatttaac cttctggcta aattcagtaa
     1441 ctggggaccc agaagtaaat ttgaggaaga agatttcgag atcatggcgg aattgggatt
     1501 cgatttcgcc cgaattccga tgtcctattg gtgctggtct tctaaagatg actggtaccg
     1561 cgttgatgaa gatgttttaa aagatataga cgaagtaatc gagttcggga aactgtacaa
     1621 tattcacatc aatctgaatt tgcatcgcat tccggggtat tgcattaatg gacgcgatca
     1681 ggaaccgatg gacctctacg aagatacgcc cgaaagaatg caaaaggctc tggatgcggc
     1741 tatttttcac tggaaaatgt ttgctaagcg ctataaaggc atcccaaatt ctcggcttag
     1801 ttttgattta attaatgaac caccgcatac caaagaagaa acgcgccatg tagaagtcgt
     1861 tacggctttg gttaatggga ttcgcgagga agacccgcat cggttgatcg tggctgatgg
     1921 aaaagacgtt ggtcgcacgc cgatctttgg cattgctgat ttgggagttg ttcaaagtac
     1981 gcgcggctat gatccaatga gcgtcagtca ttataaagcg aattgggtac cgaaagaatc
     2041 ctatcagact ttcaagaaac caacctggcc gctcaaaggc gatgatggca ccatctggga
     2101 taaagcaatg ctgaaatctc agttaattga tccctggatg ccattagtag caaagggagt
     2161 tccagttcac gttggagagt ggggctgtta taatcaaacg ccacatgatg tagctctgcg
     2221 ctggatgcgt gatttgttat cactgtggca agaagtcggc tgggggtacg ctctttggaa
     2281 tctaaaaggt gattttggga ttctcgatag taaacgcaaa gatgtgaaat acgagcaata
     2341 taaaggacat gatctcgatc gcaagatgct ggaactctta aaggaatttt aagtgcgcta
     2401 tggaggatgt aaaattccaa taattaaaaa cttgagaatc cggaatgata taaaaactcg
     2461 atccatcata ttgcttataa ttatcagaaa taaatgtgga attagtccta cctgccgaat
     2521 gtattctggg tatttttcat aaaaatggtc ttagcagatc aaggaatggt ttagaagaaa
     2581 tagtcaaatc taaaaggaga atcaaaagat gagtccgcta tataagatta tgttactttt
     2641 tatcgtatcg ttagcaatcg cgcaaccatc acgagctaag caaaactaca agaatttcaa
     2701 attggcgatc tactgtccgg cacgcgatgt gcaaagaatg gctgatttaa actggctgga
     2761 aaagaacttc aaccttctcg ataaatacct tgatattgat aaagtttacc ttgagactta
     2821 tcgcggtcag acgttggttg ataaagagac tatttcaaaa gccaaaaatt ttttcaaaga
     2881 acgcggtatc cagacttctg gtggaattac ctatgtctgg aacgacgatc agcaattttt
     2941 atcgttttgt attactaatc cggatcagcg taaaagaatg caagaggttg ccgaatatac
     3001 cgccagtatg tttgacgaaa ttatgctgga tgatttctgg tttctaaaca ctaaaacgga
     3061 gtctgatatt caagccaaag gtgaccaaag ctggacggat ttccgtttgg agcaagcggc
     3121 ggaagcctct caaaaactga ttattaatcc ggcgaagaag gtcaatccta aggtaaaaat
     3181 aatcatcaag tatccgaact ggtatgatca ttttcaacac tgtggattta atctggagaa
     3241 acagccggct caatttgatc tgatctatac cggcacggaa accagagacc cggtttatac
     3301 tcatcagcat ttacaagcct atgagagtta tgccattatg cgctattttg aaaatattgc
     3361 gcccggacgg aatggtggcg gctgggtgga cccttttaat cgtcgcacac tcgatcgtta
     3421 tgcggaacaa atcaacatga cattgtttgc caaagcccga gaattaaccc ttttctgctt
     3481 tggagcgttg ctggaagcaa ttcctcagga agatggctcg ttcagaccct tgagtccggt
     3541 tgcgccagtc gccggatata atctggataa aattgactgt tttctaggga aattaggtca
     3601 gccgttcggc attaaaacct ataagcctta ccattcctcc ggtgaagatt tccttcccaa
     3661 ttacattggt atgctgggaa ttccgatgga aattactcct gattttccgg tgaatgatga
     3721 ggtgattttc ctgaccgaac aggctagctt cgaccctaag attattgaaa aaatcaagaa
     3781 tcaactgatt aaaggtaaag acgtagtcat cacttccgga ctttatcgtg cgctccaggg
     3841 gaaaggtatc gaggatatcg tcgaattaga gtacactgat aagaaagcac tcgtgcatag
     3901 attttacgat tggcagaatg tcttcattgc cgaaaatgat attctgattc ctcaaattcg
     3961 ctatgctacc aatgattcct gggaact
//
LOCUS       DAOSMP010000103         3986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_22766_length_3986_cov_15.304248, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000103 DAOSMP010000000
VERSION     DAOSMP010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22766_length_3986_cov_15.304248"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="PKX09_09825"
     CDS             complement(<1..877)
                     /locus_tag="PKX09_09825"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOU18159.1"
                     /translation="MTIFWLVIYNAIVMPIAYSGLMILSHFNSKIKEGLEGRQTLWLN
                     LQKFREEFPKRTVYLVHSASLGEFEQAKPVIRGLKTLRPDILLVASFTSPSGFKNAER
                     MPEVDLFTYLPIDTFLQTRRFLQKLRPTKIIFVTYELWPNLIFHANRYKIKTYLISAR
                     IRPDSSKLKPIIRHFFADLYRSLDYIYAISEEHKQSLLKLIGSVKITVLNLGDTRYDQ
                     VIERSKLLANKMVTRVFKEGFVFIGGSIWPQDARPLLPALYQCLKNFPDFKIILTPHE
                     PNDYALEMLEDEFQTH"
     gene            complement(874..1917)
                     /locus_tag="PKX09_09830"
     CDS             complement(874..1917)
                     /locus_tag="PKX09_09830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOU18160.1"
                     /translation="MTTTTNPNSILIMRLSSMGDIVVLTPIFRSLREKYPLARIDFLT
                     KMEFAEIVANNPFLSNIITYDSGTKLAGWRDLCRQLKAQEYDLLIDMHNNIRSHILSH
                     YLRGTSLLRYHKPYLKRFLLFYFWLNFFSHDFRLLKEYYKVLQPLGINYPPTAKPEIF
                     LEPEAVTKAQGILAKYEISEPFIIILPIATWPNKLFPLEKYINVARRINTDLQMPVLW
                     LGGKDDIHLQRLDYLVPGKSVRLAGETTLSESLALISLSWAVIGNDTGLTYAAEALGK
                     PVVLILGPTARETGAGCYAPESIVLEAQLFCRPCSQKGDRHCYRRQRQLCFNQITDAQ
                     VYGALVKIIGAES"
     gene            complement(1914..2894)
                     /locus_tag="PKX09_09835"
     CDS             complement(1914..2894)
                     /locus_tag="PKX09_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOU18161.1"
                     /translation="MPPKDLNNIFAPISTELDLFQKEFRQALYSEVELINQIGSYILS
                     GSGKYIRPALVLLSANLCGKSNEHSIIAASVVEILHTATLVHDDIIDDSEFRRGRPSV
                     KAIWSNKIAVLIGDFLFSRALSNMLRLQDFTVLELLSQTSELLSSGELLQLEKSLNNN
                     MDEEIYYQVIWAKTASLFATACQAGALTVAANEEQTKALFDYGRCLGLAFQIEDDLFD
                     CTGTSIHTGKPVERDIKQNLVTLPLIHALKTLAPEDRHRIRQQLKNRPSPEQVTAITE
                     LIINTNGINYAENKCRELIQQAVVALAIFPDSQYKSALINLANYTLTRNN"
     gene            complement(2901..3674)
                     /gene="tatC"
                     /locus_tag="PKX09_09840"
     CDS             complement(2901..3674)
                     /gene="tatC"
                     /locus_tag="PKX09_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOU18162.1"
                     /translation="MAQINSTNAEKEMGFLDHLEELRWRIIKILIALVLGTVVAFLFS
                     DQAIDILMRPARQVGSEMTVQVLKIQGLLMLKFKVAFLLGLVITLPIVAYQFWAFISP
                     GLLPNEKRWGPGLIIGVTVFFLLGAIFAFLVLIPIALKFLIGIGVAGVERNISIEYYT
                     KFVMQLILATGLIFQMPVLSFILTKIGLITPQFLRKSWRYAIIILMVIAAIITPPDPV
                     SMIFMTLPLLFLYEISIWISRIASRPKIQNETDQIELAG"
BASE COUNT         1201 a          794 c          887 g         1104 t
ORIGIN      
        1 tatgcgtctg aaattcatcc tccagcattt ccaaagcata gtcattgggt tcatgtggtg
       61 ttaggataat tttaaaatca ggaaaattct tcagacattg gtagagagct ggtagaaggg
      121 gacgggcatc ctgaggccaa atactgccac caatgaaaac gaacccttct ttaaaaactc
      181 gcgtaaccat tttattagcg agtaatttgg agcgctcaat aacctgatca tagcgagtgt
      241 caccgaggtt cagcacagta atcttgactg agccgattag cttcagcaac gattgtttat
      301 gttcttccga aatagcgtaa atataatcca gcgaacggta gaggtcagcg aaaaaatgcc
      361 gaatgatcgg ctttagtttg gaactatctg gacgaattcg tgctgaaatc agataagttt
      421 ttattttata tcggttagca tggaaaatga gattcggcca caattcgtaa gtcacaaaaa
      481 taattttagt cggtcgtaat ttctgtaaaa agcggcgggt ttgtaagaaa gtatcaattg
      541 gaaggtaagt aaataaatca acttctggca ttcgttcggc atttttgaat ccactgggcg
      601 aagtgaaact ggctaccaac aaaatatccg gacgcagggt tttcaagccg cggatcaccg
      661 gtttggcttg ctcgaattca cctaaggacg ccgagtggac taaatagacc gtacgtttag
      721 gaaattcttc ccggaatttc tgtaagttta gccagagagt ctgacgtcct tccagtcctt
      781 ctttaatttt ggaattaaaa tgtgatagaa tcatcaatcc agaataagca atgggcatca
      841 caatagcatt atagattacc agccagaata tggtcaagac tcggctccta ttatttttac
      901 cagggcaccg taaacctgcg catcggtgat ttgattaaaa cataattgcc gttggcggcg
      961 atagcaatgc cggtcacctt tttgagagca aggacgacaa aatagttgag cttccaggac
     1021 aatactttct ggcgcataac agccggcgcc ggtttcccgc gcagtcggtc ccagaatcaa
     1081 taccaccggt tttcctaacg cctccgccgc ataggtcaag ccggtatcat taccgattac
     1141 cgcccagctg agactaatca gcgccagaga ttcgcttaac gtagtttcgc ctgctaaacg
     1201 aacactctta ccaggaacta aataatcaag gcgctgcagg tgaatatcat cttttccacc
     1261 aagccagagt accggcatct gtaaatcggt gttgattcgg cgcgccacat tgatatattt
     1321 ttcgagagga aatagtttat ttggccaagt cgcaatgggc aaaatgatga tgaacggctc
     1381 ggaaatttca tatttagcaa gaataccctg cgctttggta actgcttccg gctcgagaaa
     1441 aatttccggc ttggcggtcg gcggatagtt aatcccaaga ggttgcaaaa ctttatagta
     1501 ttctttcaaa agacggaaat cgtgcgagaa aaaattcagc cagaagtaga aaagcagaaa
     1561 acgtttcagg taaggtttat gataccgcag aagagaagta ccccgtaaat aatgagaaag
     1621 aatatgactg cggatattat tgtgcatatc aatcaacaaa tcgtattctt gggctttcaa
     1681 ttggcggcat aaatctcgcc aaccagccaa ttttgtcccc gaatcgtaag tgatgatatt
     1741 tgatagaaaa ggattattgg cgactatttc agcgaattcc atcttggtca gaaaatcaat
     1801 gcgggctaaa ggatatttct cccttaaact acggaaaatt ggagtgagaa caacgatgtc
     1861 acccatcgaa ctcaagcgca tgatcagaat agaattggga ttagtagtcg ttgtcaatta
     1921 tttctcgtaa gcgtgtagtt agccagatta attagggcag atttatattg agagtcgggg
     1981 aaaatagcaa gtgccaccac ggcttgctgg atcaactccc ggcatttatt ttcagcataa
     2041 ttaatgccat tggtgttgat gatgagctcc gttattgcgg tcacctgttc gggagaaggg
     2101 cgatttttca actgctggcg aattcggtgg cggtcttcgg gcgctaaggt tttcaaagcg
     2161 tggattagcg gcagagtaac taagttttgt ttgatgtctc gctctaccgg tttgccggta
     2221 tgaatagatg tgccggtgca atcgaaaaga tcgtcttcga tctggaatgc caatccgaga
     2281 cagcgaccat aatcgaagag agccttggtc tgttcttcat tggcggcaac cgtgagagcg
     2341 cccgcctgac aagcggtggc aaaaagtgaa gcagtcttag cccagatcac ttgatagtat
     2401 atttcctcat ccatattgtt gttcaggctt ttttccaact gtaataattc accggaactg
     2461 agcaattcgg aagtttgaga aagtaattcg agaactgtaa aatcttgtaa ccttaacata
     2521 ttgcttaaag ctcggctaaa aaggaagtct ccgattaata cagcaatttt attcgaccag
     2581 atggctttga cagaaggccg cccgcgacgg aattccgaat cgtctataat atcgtcatga
     2641 accagagtgg cggtatgcag aatctcgacg acactggcgg cgatgatcga atgttcgttc
     2701 gatttaccac agagattggc gctcagcaga accaatgccg gccgaatata tttgccgctt
     2761 cctgataata tataactgcc gatctggttg attaattcca cttcggaata taaagcttgt
     2821 ctgaattctt tttggaacag atcgagctca gtggaaattg gtgcaaatat gttatttaaa
     2881 tctttaggcg gcattatgaa ctatccagcc aattctattt gatcggtctc attttgaatt
     2941 ttcgggcgac tggcgatgcg cgaaatccag atgctgattt cgtatagaaa taataaaggg
     3001 agggtcataa aaatcatact aactggatcg ggtggagtaa taatagcggc aataaccatt
     3061 aaaataatga tggcataacg ccacgatttg cgcagaaatt gcggcgtaat tagcccgatt
     3121 ttggtaagga tgaaagacaa aactggcatc tggaaaatca atccagttgc gagtatcaac
     3181 tgcatgacga atttcgtgta atattcgatc gaaatgttgc gttcaacacc agctacacca
     3241 atcccaatca gaaatttgag agctatcgga attagaacaa ggaaggcaaa aattgcgcct
     3301 aatagaaaga atacggttac tccgattatt aatcctggac cccaccgttt ttcatttggc
     3361 agaagacctg gcgagataaa tgcccagaat tgataggcaa ctatcggtag agtaattacc
     3421 aaaccgagta gaaaggcaac cttgaatttc agcatcaata aaccctgaat tttgagaacc
     3481 tggacagtca tctcggagcc aacttgccgc gccggacgca ttaaaatgtc aattgcttga
     3541 tcagaaaata ggaaggcaac cacggtgccc agcaccagcg caatcaaaat tttaatgatc
     3601 cgccaacgca gttcttcgag gtgatccaga aatcccattt ctttttcggc attggtagaa
     3661 ttgatttgcg ccattgttat tggtaaactt aataaatact cataattttt ttcaaatgcg
     3721 gggtaaatat aaatatccta cacgtgaaag tcataaatta ttacttagcc ttatataata
     3781 gagaatgtct atcttatcag taaaattagc agggttcgag agatttgtat ttatttatta
     3841 tcaaattatg ggcgaagtat ctaattgtta tttttataaa taaaatattt ttctttgaat
     3901 taaccgatta aaattacgca gtcgtcacgc gcttaaggag gtcgtgcttc agctgaattg
     3961 tttaacgaat ccgtcgtagt tgttta
//
LOCUS       DAOSMP010000104         3955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_22983_length_3955_cov_16.099487, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000104 DAOSMP010000000
VERSION     DAOSMP010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22983_length_3955_cov_16.099487"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /locus_tag="PKX09_09845"
     CDS             complement(<1..1249)
                     /locus_tag="PKX09_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOU18163.1"
                     /translation="MKLKKYSLGIGDRFGHQAEAQLTAIIKAAEKDVNITPVWNKSFR
                     EHNIIGSQPADVRYLAEKAVQALNWSKGYFVDADHINLKTVPHFIESSDFFTLDVADA
                     IGVPPNENDIREFLKFAEKYTRPFGIPGIPQKIAVDHKSIEKIARKYLAAVLAAADTY
                     HYIQDNCQHDYVIELSVDETDKPQSPLELFFILSMAAWQELPLATIAPKFSGRFNKGV
                     DYVGDPAKFAVEFEQDVAVVKFAIKEFSLPDGLKLSVHSGSDKFSLYPHINSIITKTN
                     AGLHLKTAGTTWLEELIGLATAGGEGLEIAREIYCRAYERYEELVKPYATVVDIDRTK
                     LPVPSVVNGWTSREYVSALRHNQSEKAYNPHLRQLLHVGYKIAAEMGERYLSDLNKYA
                     KFIAPNVTENLYQRHIQPLFGVPV"
     gene            complement(1351..>2354)
                     /locus_tag="PKX09_09850"
     CDS             complement(1351..>2354)
                     /locus_tag="PKX09_09850"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4861 domain-containing protein"
                     /protein_id="HOU18164.1"
                     /translation="NAEKTEILFVTDFAPSEKKTFTIHYAKEGTITHNYPKRTQAELS
                     HKVGGRFVNRIYEGGQFQNVKYLRVPPEHTDHSLFIRYEGPGWESDKVGYRFYLDWRN
                     AIDVFGKKVPDMVLQEIGQDGFESYHSMCPWGMDVLKVGESLGLGSLGMWYEGKAERI
                     AVTDSVICEILQDGILESRIRTRYYGWQIGGQKYDLVSELSIAAGSRLTRHDIQINDE
                     PPNLCTGIVKMENVSLLSSTDQNRDWMYFATYGKQSLANDKLGLAVLFRRQDLIQITD
                     DENSYVVILKPTVGKLTYYFLAAWEQEPDGIKTESQFADYLNETVAMLDNDLIITIK"
     assembly_gap    2355..2454
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2455..3580)
                     /locus_tag="PKX09_09855"
     CDS             complement(<2455..3580)
                     /locus_tag="PKX09_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOU18165.1"
                     /translation="MRKIVVMTFLLICLVQIGTASEDFDNLMIVDQSGAGQFTSITAA
                     LAALPDMAYQRVVIFIRNGIYEEKIRLDQNYVTLRGENRDSTIIRFCQKRTDWEANKD
                     YIGPAVVNIYADDIILENLTVENTQPEIEPHAFAIYGKGTRTILRNCNLLGKGGDTVS
                     LWNYQQGLYYHSNCYFRGAVDMVCPRGWCFIRDSQFFEVKTSATIWHDGHYDPDQKFV
                     IVNSTFDGVPDFQLGRHHYDAQFFLINCTFSENMADQPIYLKTYDDVSRNNPYLWGER
                     KYFFNCQKAGKAFDWYRNNLATAPGKPQPEEITPAWTFADRWDPESSAPVTVTAYRID
                     GRTVELTFSESVTVGGNPIFMSQTGFKFSIMPQRFTDNCRL"
     gene            complement(3650..>3955)
                     /locus_tag="PKX09_09860"
     CDS             complement(3650..>3955)
                     /locus_tag="PKX09_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOU18166.1"
                     /translation="NCGAVLPKRDAVDERLVAEVRDGVVHFGKNGIIDSQKEVGGWPA
                     LNSTTPVNDIDQDGMADDWELANGLNPNDPNDRNGDFDGDGYTNLEEYLNSLDKLFY"
BASE COUNT         1081 a          942 c          773 g         1059 t
ORIGIN      
        1 ccacgggcac cccaaacaac ggctgaatat gtctttggta gagattttcg gtcacgttcg
       61 gggcaataaa tttagcatat ttattgagat ccgaaaggta gcgctcgccc atctcagccg
      121 caatcttata gccgacgtgc aacagctggc gcagatgcgg attataggct ttttcactct
      181 gattatgacg taaagccgat acatactcgc gagaagtcca gccattgacg accgaaggca
      241 ccggtaattt agtccgatca atgtccacaa cagttgcata gggcttaact aattcctcat
      301 aacgttcata tgcacggcaa tagatttcac gcgctatttc caagccttcg ccgccagccg
      361 tagctaaacc aatcagttcc tccagccagg ttgtcccggc agtcttgaga tgcagtccgg
      421 cattagtttt tgtaataatg ctattaatat gtggatagag cgagaactta tcactgccag
      481 agtgaacgct aagtttcagc ccatccggca aagaaaattc cttgatcgca aattttacca
      541 cggcgacatc ctgctcgaat tcaaccgcaa attttgccgg gtcgccgacg tagtcaacgc
      601 ctttattgaa acgtcccgaa aatttcggcg caattgtagc cagcggtagc tcctgccaag
      661 ccgccatact cagaatgaaa aataattcca acgggctttg tggcttgtca gtttcatcaa
      721 cagaaagttc aattacataa tcgtgttgac aattatcctg aatgtagtga taagtatcag
      781 ccgcggctaa tactgccgct aagtatttgc gggcgatctt ttcgatagat ttatgatcaa
      841 ctgcgatttt ctgcggaatg cctggaatac caaaaggtcg cgtgtatttt tcagcaaatt
      901 ttaaaaattc ccgaatatcg ttttcattgg gcggaacacc gatggcatca gccacatcca
      961 gcgtaaaaaa atcagaggat tcgataaaat ggggcacagt tttgagattg atatgatcgg
     1021 catcaacaaa ataccccttc gaccagttca aagcctggac tgctttctcc gccaaataac
     1081 gcacatctgc cggttgactg ccgattatat tatgttcgcg aaaagactta ttccagactg
     1141 gcgtgatatt gacgtccttt tcggcggctt tgattatcgc ggttaattgg gcttcagctt
     1201 gatgcccgaa acgatcccct attcccagtg aatatttttt caatttcatt ttgactattt
     1261 ccttttagca attaaaaaaa ccgatctgta tttacgaatt cttacgaatt taaaaaataa
     1321 aaattccggc aattaatgtt attcgatgtt ctattttatc gtaataatta aatcattatc
     1381 cagcattgcc accgtttcat tcagataatc tgcgaactgg ctttcggttt taatgccgtc
     1441 cggttcctgt tcccaggcgg ctaagaaata atacgtcaat ttccccaccg tcggtttcaa
     1501 aatcacaaca tagctatttt catcatcggt tatctggatc aaatcttggc gacggaaaag
     1561 caccgccagc cctaatttat cattcgccag actctgttta ccataggtgg caaagtacat
     1621 ccagtcacga ttttgatcag tcgaactaag tagcgacacg ttttccattt tcacgatgcc
     1681 ggtacacaga ttgggcggct cgtcgttaat ttggatatcg tgacgggtca agcgactacc
     1741 ggcggcaatc gagagttcgg acaccaaatc atatttttgg cctccgattt gccagccata
     1801 ataacgggta cggatgcgcg attcaagtat gccatcctgt aaaatttcgc aaattacact
     1861 gtcagtgacg gcaatccgct cggctttacc ttcataccac atacccaacg aaccaagacc
     1921 gagcgattcg ccgactttca gcacatccat tccccaggga cacatactat gatacgattc
     1981 aaagccgtcc tggccgattt cctgtagaac catatcgggg actttcttgc caaagacgtc
     2041 tatggcattg cgccagtcga gataaaaacg atagccgact ttatcggatt cccatcccgg
     2101 tccttcatag cgaatgaaca aggaatgatc ggtatgttca ggcggtaccc gcagatattt
     2161 tacattctga aattgtccac cttcataaat ccgattaaca aaacgtccac cgactttatg
     2221 agataattcc gcctgcgttc gcttgggata attgtgagta atcgttcctt ccttagcata
     2281 atgaattgtg aaggttttct tctcggaagg agcaaaatcg gtaacgaata aaatttcggt
     2341 tttctccgca tttannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcaagc
     2461 ggcaattgtc ggtaaaacgc tgaggcataa tgctaaattt aaagccggtc tgactcataa
     2521 aaatcggatt gccacctacc gtcacacttt cactaaaagt taattcaact gttcgaccat
     2581 caatgcgata agctgtaacc gtaacaggcg cgcttgattc cggatcccag cgatcagcaa
     2641 aagtccaggc tggtgttatt tcttccggtt gcggttttcc aggggctgtc gcgagattat
     2701 tacgatacca atcgaaagct ttgcccgctt tctggcaatt aaagaaatat ttacgttcgc
     2761 cccacaaata aggattgttg cgactgacat cgtcataagt tttcagatag atcggctgat
     2821 ccgccatatt ttctgaaaaa gtacaattga ttaggaaaaa ttgagcgtcg taatgatggc
     2881 gtcccagctg aaaatctggc acgccgtcaa aagtggaatt tactataacg aatttctgat
     2941 ccggatcgta atgaccatca tgccagatcg tcgccgaggt ctttacctcg aaaaattgcg
     3001 aatcgcggat aaaacaccac ccgcgcgggc aaaccatatc aaccgccccg cgaaaataac
     3061 aattagaatg gtaatacaaa ccctgctgat aattccagag cgaaacggta tcgccgcctt
     3121 ttcccagcag gttgcagttc cgtaaaattg tgcgagttcc ttttccataa atagcgaagg
     3181 catgcggttc aatctccggc tgagtatttt ccaccgtcaa attttccaaa ataatgtcgt
     3241 cagcatagat attgacgact gccggtccga tataatcctt gttggcttcc caatcagtgc
     3301 gtttctgaca gaaacggata atggtgctgt cgcgattttc accgcgaaga gtgacataat
     3361 tctggtctaa tcgaattttt tcctcgtaaa taccatttcg aataaaaata accacccgtt
     3421 gataagccat atccggtaaa gccgccagcg ccgccgtgat cgaggtaaac tgtcccgcac
     3481 cactttgatc aacaatcatc agattatcga agtcttcaga ggcagtccca atttgaacca
     3541 gacaaatcag cagaaacgtc atgacgacaa tttttctcat aaaagacctt cagttataac
     3601 atcgctacca atttcctcaa tcactacatt tatctttttc agaagcattt taataaaata
     3661 atttatccag actatttaaa tattcttcaa gattagtgta accgtcaccg tcaaaatcgc
     3721 cgttacggtc gttcgggtcg ttgggattaa gaccatttgc cagttcccaa tcgtctgcca
     3781 taccgtcctg atctatatca ttgaccggtg tagtagaatt caaggccggc cagccgccaa
     3841 cttccttctg agagtcaata ataccatttt tgccgaaatg aacgacccca tcgcgaacct
     3901 ccgccaccag acgttcatcc accgcatcgc gtttgggcaa aacagcaccg cagtt
//
LOCUS       DAOSMP010000105         3933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_23139_length_3933_cov_15.981692, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000105 DAOSMP010000000
VERSION     DAOSMP010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23139_length_3933_cov_15.981692"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PKX09_09865"
     CDS             <1..603
                     /locus_tag="PKX09_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOU18167.1"
                     /translation="LCALSHQYQTALVFGMAEKSGDQIFNSAVVLDKGEIRAVYRKVH
                     LFYEEKKLFSAGTETPPVIEIQGVKIGVMICFDWFFPEMARTLALSGAQIIAHPANLV
                     LPYCQAAMLTRSLENRVFTATANRIGSERCGTKRLTFTGKSQITSPRGELLGQLKTGR
                     PGILARDIDPTEALDKSLTPLNDIFEDRRPEVYQLGKSSR"
     gene            600..881
                     /locus_tag="PKX09_09870"
     CDS             600..881
                     /locus_tag="PKX09_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18168.1"
                     /translation="MKKSLANPEELSLENQRMLWLRVYTDRVIRQLQTMSLDEPEAVS
                     LIEQVKQEILRRFPDKERAYRLIYERRFKRILQRRGLFLPLTMDNGEIN"
     gene            902..1462
                     /locus_tag="PKX09_09875"
     CDS             902..1462
                     /locus_tag="PKX09_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOU18169.1"
                     /translation="MNYPDAYELLCRYTKTESLRKHALAVSAAMRHYARIFNEDEEYW
                     AITGLLHDFDYEMYPDEQNHPYRGNEILASEGYPEEMRTAIMGHASYTGVKRESLLAK
                     TLFAVDELSGFIIAVALVRPSKSIFEVEPRSVQKKFKDKAFAAKVNREEIKQGITELG
                     VDETEHIQNVIDALKSVAAELGLAGS"
     gene            1593..>3933
                     /locus_tag="PKX09_09880"
     CDS             1593..>3933
                     /locus_tag="PKX09_09880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOU18170.1"
                     /translation="MRIILTQVEKPGRYVGNELNAIRKDHTRTAATIALAFPDLYDVG
                     MSYYGFQILYHILNRETDIAAERVYVPWPDMSRQMRSRQIKLFSLETHTSLDQFDVIG
                     FTLPHELTYANILTMLDLAQIPPKAINRKPSDPFIIGGGSGAYNPEPLAPFFDLFVIG
                     DAEEIVVPLVRLLAEGRRRGTPRRVILEEALHRFTGIYIPTFYEVENGRTVPVTSFAP
                     GVIRANKIRTLQNDSYPQAPLMPLIEIAQDRLVIELMRGCTEGCRFCQAGIIYRPVRE
                     RQPADIIRQIDQSLPQTGYDEVSLLSLSTSDYSRITPLLTYLEPALESQKIGLSYPSL
                     RLDSFGETVARLGTATRRSGLTFAPEAGSERLRRVINKQITEDNLLKAVELACRYGWR
                     LLKLYFMLGLPTETEADLEAIISLVQKVLAVSGKRLELNITLSTFIPKPMTPFQWVAM
                     DEPLAIQRKLDYLKPRLRTLKNIKVMARDPRASELEGALARGDRRLAHVIETAWQAGA
                     GFDGWQEHFDPVKWDEAFRLNNLDKRVFTGELETEKALPWEHIDCGVSREYLFDEYRK
                     VGQESNTSDCRYGCNQCGVCFSGGSEMILAEEWLPPQTNPLIIKTDSEIIPIRYRLHY
                     RKFGNAVFTSHRDILRIMPGILRRAGLEPVFTQGFNPRPKLSAGYPLPFGYASEDEVL
                     DIYLKNAIENSAERLNRALPDGFEVVKGTEVSLQLPSVFAATTGFQYLVQINNCSDRE
                     LLRKIEEFLTEREIEFQRETSNSQQKINLRSFVETIRCEG"
BASE COUNT         1073 a          882 c          969 g         1009 t
ORIGIN      
        1 ctctgcgcat taagccacca atatcagact gcgctggttt tcggaatggc tgaaaaaagc
       61 ggcgatcaaa ttttcaatag cgccgtggtg ctcgacaaag gcgaaattcg ggcggtttat
      121 cggaaagtgc acctttttta tgaagaaaaa aaattgtttt ctgcagggac agaaacgcct
      181 ccggttattg aaattcaagg cgttaaaatt ggtgtgatga tttgttttga ctggtttttc
      241 cctgaaatgg cacgcactct ggctctgtcg ggcgctcaaa ttattgctca tccagccaat
      301 ctggtgttgc cctattgcca ggcggcgatg ctaactcgta gtctggaaaa tcgcgttttt
      361 accgcgactg ctaaccggat cggcagtgaa aggtgtggca caaaaaggct aacttttacc
      421 ggaaaaagcc agataaccag tccgcgtggg gaactgttgg gacaactaaa aactggtcgc
      481 cccggcattt tagcgcgcga cattgatccg actgaggcat tggacaaatc attgacgcca
      541 ctcaatgata ttttcgagga tcggcgtccc gaagtttatc aactgggaaa atcgtcgcga
      601 tgaagaaatc tttagcaaat ccggaagaat tgagtctaga aaatcaacga atgctgtggc
      661 tgagagtata tactgaccgg gtaatacgcc agcttcaaac tatgtcgtta gatgaaccgg
      721 aggcagtaag cctaatcgag caagtcaaac aagaaatcct tcggcgtttt ccagataaag
      781 agagagccta ccgtctgatt tacgagcgtc gtttcaaaag aatattgcaa aggcgcggat
      841 tgtttttacc actaacgatg gataacggag aaataaacta agtttgttca ggagaaaaaa
      901 aatgaactac ccagatgcct atgaattgct ctgtcgctac accaagaccg agagtttgcg
      961 caaacatgcc ctagccgtct cagccgctat gcgccattac gccagaatat ttaacgaaga
     1021 tgaagaatac tgggcaatta ccggcttatt acacgatttc gattatgaga tgtatcctga
     1081 tgaacaaaat cacccttatc gcggtaatga aattttagct agtgaaggtt acccggaaga
     1141 aatgcgaacg gcaatcatgg gacatgcttc ttacaccggt gtcaaacgtg aatcgctttt
     1201 ggcaaaaacc ttattcgcag tggatgaatt gagcgggttc attatcgccg tggctctggt
     1261 gcgtccttct aagagcatat tcgaggtaga gccgcgttcg gtgcagaaaa aattcaaaga
     1321 caaagctttc gccgccaaag tcaatcgcga ggaaattaaa cagggcatca ctgaactcgg
     1381 cgtggacgaa accgaacaca ttcaaaatgt cattgacgct ttgaaaagtg tcgccgctga
     1441 attggggtta gctggaagtt gagatatgaa attcttagct gactcgatag cactaaaatt
     1501 tatcggtagt tgaagttcta ttgtttatct gccattcaga attgttatta ttgtattgaa
     1561 aattgaaaac cattactaat tcgaacgacc tgatgagaat tattctaacg caggtcgaaa
     1621 aacccggccg ctatgtcggc aatgaactga atgctattcg caaagatcac acccgcactg
     1681 cggcgacaat tgcgctggca tttcccgatc tctatgacgt tggaatgtcg tattacggct
     1741 tccagatcct atatcatatt ttaaataggg aaaccgatat agccgcggaa cgagtttatg
     1801 tgccctggcc ggatatgagt cggcagatgc gatctcggca gatcaaactt ttcagcctcg
     1861 agactcacac cagtctcgat caatttgatg taatcggatt cactttacct catgaattga
     1921 cttatgccaa tattctgaca atgctcgatc tagctcaaat tcccccgaaa gctattaaca
     1981 gaaagccgtc cgatccattc atcatcggcg gcggtagtgg agcctacaat ccggaaccgc
     2041 tggcgccctt ttttgaccta tttgtaattg gtgatgcgga ggaaattgtg gtgccgttgg
     2101 tgcgtctgct ggcagaaggc cggcgccgag ggacacctcg tcgggtgata ttggaagaag
     2161 ctctccaccg ttttacagga atctacatac cgacgtttta cgaagttgag aatggacgaa
     2221 ctgttccggt gacatccttt gcaccgggag ttatccgggc gaataaaatt cggactttgc
     2281 agaatgactc ttacccacaa gctccattaa tgccattgat tgaaatcgct caggatcggt
     2341 tagtaatcga attgatgcgg ggatgcactg agggttgtag gttttgccaa gccggaatta
     2401 tttatcgtcc ggtgcgcgag cgccaaccgg cggacattat ccggcaaatt gaccaatctt
     2461 tgcctcaaac cggttacgac gaagtatcac ttctgtcgct atccacctcc gattatagtc
     2521 gcattacgcc gctcttaact tatttggagc cagccttgga atctcagaaa atcgggcttt
     2581 cctatccatc acttcgactc gacagtttcg gggaaaccgt tgccagattg gggacagcta
     2641 ctcgccgctc gggattaacc tttgcgccgg aagccggttc ggaacgatta cgtcgcgtaa
     2701 tcaacaaaca gataactgaa gataatttac taaaagcggt ggaattggct tgccggtatg
     2761 gttggcgctt gctgaaactc tattttatgc ttggtctgcc aactgaaact gaggctgatc
     2821 tggaggcgat aatttctctc gttcagaagg ttctggcagt tagcggtaaa cgtctggaat
     2881 tgaatatcac gctttcgact tttattccta agccgatgac gccattccaa tgggtggcga
     2941 tggatgaacc actcgcaatt cagcgtaagc ttgattattt aaaaccacgt ttacgcaccc
     3001 tgaaaaatat caaggtaatg gcgcgcgatc cgcgggcctc agaattggaa ggcgcattgg
     3061 cgcgtggcga tcgccgcctc gcccatgtca ttgaaacagc ctggcaggca ggagctggat
     3121 ttgacggctg gcaggaacat tttgatccgg taaaatggga cgaagctttc cgccttaata
     3181 acctcgataa gcgagtgttt accggagaat tggaaacgga aaaagcattg ccatgggaac
     3241 atattgattg cggggtaagt cgtgaatatt tattcgatga ataccggaaa gtcggacagg
     3301 agagcaacac ttcagactgc cgctatggtt gcaatcaatg cggcgtttgt tttagcggcg
     3361 gctcggaaat gattcttgcc gaagaatggc tgccgcctca gaccaatccg cttattataa
     3421 aaaccgattc ggaaataatt ccgatacgat accgcttaca ttatcgtaaa tttggtaatg
     3481 ctgtattcac ttcccatcgg gacattttgc gtataatgcc gggtattctg cgacgcgccg
     3541 gactggagcc ggtttttacc cagggtttta atccgcgccc gaagctgtca gccggttatc
     3601 cgctcccgtt tggctacgcc tcggaggatg aggtactgga tatttatttg aaaaatgcta
     3661 tcgaaaatag cgcagaacga ctgaaccgag ccctacctga cggattcgag gtagtcaaag
     3721 gcacggaagt ttctttgcaa ttaccgtcgg tatttgcggc taccaccggt tttcagtatt
     3781 tagtccagat caataactgt agtgatcgcg agctattacg gaaaatagag gaattcctga
     3841 cggaacggga aatcgagttc cagcgcgaaa caagcaacag tcagcaaaag atcaacctgc
     3901 gttcgttcgt tgaaaccatc cggtgcgaag gta
//
LOCUS       DAOSMP010000106         3866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_23641_length_3866_cov_14.561795, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000106 DAOSMP010000000
VERSION     DAOSMP010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23641_length_3866_cov_14.561795"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PKX09_09885"
     CDS             <1..782
                     /locus_tag="PKX09_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU18171.1"
                     /translation="DELFQVAGFDAVFLGVGAGLPWFMNIPGENLMGVYSANEFLTRV
                     NLMRAYEFPEADTPIKIGKQVVVVGGGNTAMDAVRTARRLGAEKATIVYRRSEVEMPA
                     RLEEIKHAKEEGVIFRTLTNPIEFLPDENGWVRAARCLQMELGEPDSDGRRRPIPIPN
                     SEFEIPTDLVIMAIGNGSNPLISRTTPDLQCNKWGNIITSGEGGRTSKRGVFAGGDIV
                     TGGATVILAMGAGRAAAHSIDEYLKTGIWATEPDSANQNKT"
     gene            790..1974
                     /locus_tag="PKX09_09890"
     CDS             790..1974
                     /locus_tag="PKX09_09890"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOU18172.1"
                     /translation="MPISNYIAQSMTRSSWIRKMFETGIALKKQYGADKIYDFSLGNP
                     DLPTPEIFNRVLVEESARTGTYVHGYMQNKGYPETCAAVANLLKRESGLPFTEDQVVM
                     TCGAAGAINIILKSLLDPGDEVIVIAPFFSEYEFYVDNHRGQLVISEANAELLPDPAD
                     IAAKITPRTRVLLLNSPNNPTGRVYGEDIFREIGKLLTEKSAEFGRPIYLLMDEPYKR
                     IIFDGLQYRSPFRYYRHTILASSFSKDLSLAGERIGYLAISPLIEDYPALINAAAFCN
                     RVLGFINAPALMQRVIRRVLDAQVAIELYQKRRDMIYDALVKIGYEIIKPEGTFYLFP
                     KCPIDDDVKFVFALQDRLIITTPGIAFYRPGYFRISYAVPEAVIQATIPIFKQVFNEM
                     KP"
     gene            1993..2232
                     /locus_tag="PKX09_09895"
                     /pseudo
     CDS             1993..2232
                     /locus_tag="PKX09_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
     gene            2394..3446
                     /locus_tag="PKX09_09900"
     CDS             2394..3446
                     /locus_tag="PKX09_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOU18173.1"
                     /translation="MAKQFLKGNEAVVKGAVLAGCQAYYGYPITPASEIAHAAALYLP
                     VSGGTFLQAESEIAAINMVYGAAAAGQRVMTASSGPGISLKQEGISYCAGSELPCVIV
                     DVMRGGPGLGNIAPEQGDYNQVVKGGGHGNYKCIVLAPNSVQEMCDFGMLAFDLADKY
                     RNPVYILSDGFVGQMMEPVEFPEPVKQFPEKSWAIKGTPETSDNLITSIELDPDDLER
                     HNLKLQQKYAIIEEQEVRFEDYSLSDAEIVLVGFGIISRILQTIVDNLRDVGVKVGLL
                     RPITLFPFPKKKISQLAEQAKFFLVVEMSNGQLVDDVRLAVAGRKPVYLYNRLGGNVP
                     SEKEINDELLKLMRSV"
     gene            3443..>3866
                     /locus_tag="PKX09_09905"
     CDS             3443..>3866
                     /locus_tag="PKX09_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU18174.1"
                     /translation="MNNILQKSAIFYDVFERKPGTDKLTTTYCPGCGHGNLHKLIAEA
                     LVDFGVQDRTIFISPVGCSVFAYYYFHTGNVQVAHGRAPAAATGLKRAHPEAIVISYQ
                     GDGDLAAIGTAEIIHAANRGEGITVFFVNNAIYGMTGGQ"
BASE COUNT          995 a          872 c          959 g         1040 t
ORIGIN      
        1 tagatgaact ttttcaagtc gctggatttg atgcggtttt cctgggtgtc ggtgcaggtt
       61 taccctggtt tatgaatatt cccggtgaga atctaatggg agtctattcc gccaatgaat
      121 ttctaacgcg ggtgaatttg atgcgcgctt atgaatttcc tgaagctgac accccgataa
      181 aaataggcaa acaggtggta gttgtcggcg gcgggaacac cgctatggat gccgtccgta
      241 ccgccagacg tctcggagct gagaaagcca cgattgttta ccgccgctcg gaggtagaaa
      301 tgccagcccg cctggaagag attaaacatg ccaaagaaga aggcgtcatt tttcgaactt
      361 tgaccaatcc gattgaattt ctgcctgacg aaaatggctg ggtaagagcc gcccgctgtc
      421 tgcaaatgga actcggcgaa cccgattcgg atgggcgacg acgtcctatt cccattccca
      481 atagtgaatt tgaaatcccg actgatctgg tcattatggc aattgggaat ggttcaaatc
      541 cgctcatctc acgtaccaca cccgatttgc aatgcaataa atggggcaat atcattacca
      601 gcggcgaggg cggacgaact tccaagcgcg gcgttttcgc cggcggcgac atcgttacag
      661 gtggcgcaac cgttattctg gctatgggcg ccggccgagc cgctgcgcat tcaattgacg
      721 aatacctcaa aactggaatc tgggctacgg agccggattc agccaatcaa aataaaactt
      781 agaggtgata tgccgatttc caattatata gctcaatcta tgacccgctc atcgtggatt
      841 cggaaaatgt tcgagaccgg aattgctttg aaaaagcagt atggtgcgga taaaatttac
      901 gatttctctc tcggcaaccc ggatttgcct actccggaga tttttaatcg cgttctggtc
      961 gaagaatcag ctcgtactgg gacttatgtg catggctata tgcaaaataa gggttatccc
     1021 gagacctgtg cggcggtggc gaatttgctc aagcgagaaa gtggtttgcc gtttaccgaa
     1081 gatcaggtcg ttatgacctg cggtgcggct ggagcaatta acatcatcct caaatctctg
     1141 cttgatcccg gcgacgaagt catcgtaatt gcccctttct tttcggaata tgaattttat
     1201 gtagataatc accgcgggca attggtcatt tccgaggcaa atgcggaact attacccgat
     1261 ccggctgaca ttgccgcgaa aattacgccg cgtactcggg ttctactgct gaattctccc
     1321 aacaatccca ccggacgtgt ttacggcgaa gacatttttc gtgagattgg gaaattgctg
     1381 accgaaaaaa gtgccgagtt tggtcgcccg atttatttat tgatggacga gccttataag
     1441 agaatcattt ttgacggact ccaatatcgt tctccatttc gctattatcg gcatacaatc
     1501 ctggcatcct cattttccaa agacctttcg ctagccggag aacgcatcgg ttatctagct
     1561 atcagcccat taattgagga ttatccggct ttaatcaatg cggcggcgtt ctgcaatcgg
     1621 gtgctgggct ttattaacgc gccagcgttg atgcagagag ttattcgacg agtactggat
     1681 gctcaagtcg ctatcgaatt ataccagaaa cggcgtgata tgatttatga cgctttagtg
     1741 aaaatcgggt atgaaattat caaaccggaa ggcacttttt acctctttcc taagtgccca
     1801 attgatgacg atgtcaaatt cgttttcgct ttgcaggatc ggctgataat tacaacaccg
     1861 gggattgctt tctaccgacc gggatatttt cggatttctt atgctgtacc cgaagccgtg
     1921 attcaagcca caattcctat attcaaacag gttttcaatg aaatgaaacc ataattaaac
     1981 aatggagcag atatgaataa aacccaatca gtaccccaaa aaacggtcac gcggggacac
     2041 gttatcatca actctgaaga atgtaagggt tgtgaattat gtatcgcgtc ctgtccacca
     2101 aaggttttgt atttgactga ccgatttaat actcacggtt atcatccttc cgcttataag
     2161 ggagaaggct gtaccggctg tggaatctgc ttctataatt gtcccgaact gggtgccatt
     2221 acagttttca aacgctggga tttagtgact gagaaggctt tctgtccgga gtgcggtagc
     2281 gaacagaaag ttttttccag agagggcaat gaaaaggtaa aactctgcgc gggatgtctg
     2341 cgcccggtgt cacctgaaaa ggtcataacc attaaatagc ggaggattaa actatggcta
     2401 aacaattttt aaaaggaaac gaagccgtgg tgaaaggagc agtgctagct ggttgtcaag
     2461 cttattacgg ctatcccatc acgccagcta gtgaaattgc ccacgcggcg gctttatatc
     2521 taccggtgtc aggtggcaca ttcctgcaag ccgaaagtga aattgccgcg attaatatgg
     2581 tttacggtgc ggcggccgct ggtcagcgcg ttatgactgc ttcctccggt cctggcatta
     2641 gcctgaaaca ggaaggcatt tcctattgtg ccggttcgga attgccctgt gtcatcgtag
     2701 atgttatgcg agggggcccc gggctgggta atatcgctcc tgaacagggt gactataacc
     2761 aggttgtcaa aggtggcgga cacggaaact ataaatgcat cgtgctggcg cctaactccg
     2821 ttcaggaaat gtgtgatttc ggcatgctgg cgtttgatct ggctgataaa taccgcaatc
     2881 cggtttatat tctctcagac ggttttgtcg gtcaaatgat ggaaccggtc gagtttcccg
     2941 aaccggtaaa gcagtttccc gaaaaatcct gggcgatcaa aggtactcct gagacttccg
     3001 ataatctaat cacttcaatt gaactcgatc cggacgattt ggagcgccat aatttgaagc
     3061 tccagcaaaa atatgccatt atagaagagc aagaagttcg ttttgaagat tatagtctca
     3121 gcgatgcgga aattgttctg gtgggattcg gaattatatc gcgcattctg caaactatcg
     3181 ttgacaattt acgagatgta ggagtcaagg tcggtttact aaggccgatc accctttttc
     3241 cctttcctaa aaagaaaatc agccaacttg ccgaacaggc caaattcttc ttagtcgttg
     3301 aaatgagtaa tggccagttg gttgatgatg ttcgtttagc ggtcgctggt cgtaaaccgg
     3361 tgtaccttta taatcggctg ggcggcaatg tgccttccga aaaggaaatt aacgacgaat
     3421 tgctaaaact gatgaggtcg gtatgaataa catattgcag aaatcagcca ttttttatga
     3481 tgtgtttgag cgcaagcccg gtaccgacaa acttaccact acttactgtc ccggttgtgg
     3541 acatggaaat ctgcacaaat tgattgccga agcgctggtt gatttcggcg ttcaggatcg
     3601 cacaattttt atcagtccgg tcggttgcag tgtttttgct tattattatt ttcataccgg
     3661 taatgttcaa gttgcccacg gacgtgctcc agccgccgct actggcctca agcgcgctca
     3721 ccccgaagcc atcgtaattt cctatcaggg tgatggtgat ttagccgcaa ttggcaccgc
     3781 cgaaataatt cacgccgcta accgaggcga gggcataacg gtttttttcg tcaataatgc
     3841 gatttacggc atgaccggag ggcaaa
//
LOCUS       DAOSMP010000107         3714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_24865_length_3714_cov_12.647445, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000107 DAOSMP010000000
VERSION     DAOSMP010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24865_length_3714_cov_12.647445"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..1047
                     /locus_tag="PKX09_09910"
     CDS             271..1047
                     /locus_tag="PKX09_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18175.1"
                     /translation="MKESKIFSILVFAIAFMLTLTTNSFAQEEMPPIEEEVVEETVEE
                     EVVTEEPDVFMPSWIAKAYLNFGPIEPWGDPGSGALFNTGMRVQGGTQLNIGNWLQLP
                     MILQPLTAEVMVGYGMWNIKDDYEGSVYEEGKTNVISVLALGRYDLTDLIMKAIGVEY
                     PALGIFAVAGLQYNYQSWDFPNWSREFDPASSFGINLGVGVKYNLQSLVGKPVEIDLR
                     FTQGVFVMGDVKDQNGDPFYPDADYQHTENGLLLGIAYPF"
     gene            complement(1151..2179)
                     /locus_tag="PKX09_09915"
     CDS             complement(1151..2179)
                     /locus_tag="PKX09_09915"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOU18176.1"
                     /translation="MQRLISILRREREKAHLTQNDLALRMNCSRDTIARIETGRQVIN
                     LDQLLGYIRICQINPNVILKAFEPEKPAEFAFMLRMHNVHYITDQTRQQFADWYSACE
                     KSRTNEHIIIRELPDLKYDESQDPRHSGEHHAIVTRANWNLGSVPIQDPVSLISDLGI
                     FIQGADLGNNDLFALTGKRNDSNQYGMIINTNSAITIERQRFSIFHELAHLIAHRDDF
                     SIDYNETGKGRSKDPREIYADAFAGAFLVPQDELERFIQIANARSPLNLGSWILEAKR
                     YFGVSYQTILLRLKSIGYFKNDRQFGIIFGKLRKIYGSNEPNPITTPLSFINTPDWPD
                     TIVFKLSS"
     gene            2439..2810
                     /locus_tag="PKX09_09920"
     CDS             2439..2810
                     /locus_tag="PKX09_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18177.1"
                     /translation="MAKLILPRPALLIAQLQMMSAALAKYAETYVPVSPSKDELDACV
                     TELTSALQNQVQAAGIAEKATKDLYAVRDRSVDLLRRMRDALYAHFGKYDPRLVEFGL
                     DTMKARKPKNGNGSDETGNLQ"
     gene            complement(3083..3316)
                     /locus_tag="PKX09_09925"
     CDS             complement(3083..3316)
                     /locus_tag="PKX09_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18178.1"
                     /translation="MAKHFSSQTKLPNDLAKHFNRLTKWPNDLAKHFSSQTNWPNDLA
                     KHFTGQTKWPNDLAKHFTGQTKWPNGLSKLISE"
BASE COUNT         1130 a          696 c          763 g         1125 t
ORIGIN      
        1 atttaccgaa taaagaaata tcttattaat cctgtataaa aattcctcat taattcccta
       61 agaagaaccg tgatataaat caccatttta ctcttattca tataatatca attacttgta
      121 ttccttttat ggtaaatata ggccggataa aattatttta ttcttaaagg ctaattaaaa
      181 aattctattg acatattaaa aaactatata tatattaacc ataagttaaa atggctatga
      241 gaaaatcaat atttaataaa ggaggttatt gtgaaggaaa gtaaaatctt ttcaatctta
      301 gtttttgcta ttgcttttat gctcacttta acaaccaatt ctttcgcaca ggaggagatg
      361 cctcccattg aggaagaggt tgttgaggaa acagtcgaag aagaagtagt tactgaagaa
      421 ccagacgttt tcatgcctag ctggatagcc aaagcttatt tgaattttgg tccaatcgaa
      481 ccctggggcg atccaggtag tggagcatta tttaacacgg gtatgcgggt ccagggtggc
      541 actcagctaa acattggcaa ctggttacaa ttaccaatga ttctccaacc gttgactgcc
      601 gaggttatgg tcggctatgg aatgtggaat attaaagacg attatgaagg ctctgtatat
      661 gaagaaggta agaccaatgt catctcggtt ctggcacttg gccgctatga tctaaccgat
      721 ttaatcatga aagccattgg ggttgagtat ccagcgctgg gtatttttgc tgtggctggt
      781 ctgcagtaca attatcaatc ctgggatttc ccgaattgga gtagagaatt tgacccggca
      841 tcttccttcg gtataaatct aggagtcggg gtaaagtata acctgcaaag cctggtgggt
      901 aagccagtcg aaattgattt acgtttcacc caaggcgttt ttgtgatggg cgatgttaaa
      961 gaccagaacg gcgatccgtt ctatcctgat gctgattacc agcataccga aaacggatta
     1021 ctgcttggta ttgcttaccc attctaaggt cttttagaaa atatcagtgt taataaaaaa
     1081 agtcccgatt ttcgggactt tttctttttt taagccaatt aaaacttaat ctcaatagca
     1141 cattactgtg ctagctggat aatttaaaga cgatagtatc tggccaatca ggcgtattga
     1201 taaaactcag cggtgtggta attggattag gttcattaga accgtaaatt tttcgcaatt
     1261 tgccaaatat tatcccgaat tgacggtcgt ttttaaaata tccaatagat tttagtcgca
     1321 gtaatatggt ttgataactg actccaaaat aacgtttggc ttccagaatc cagctaccaa
     1381 gattaagggg gcttctggca ttagcaattt gaataaaacg ctccaattca tcttgaggaa
     1441 ctaaaaacgc accggcaaag gcatcagcgt aaatttctct gggatcttta gaccttcctt
     1501 taccagtttc attataatca atggaaaaat catcgcggtg ggctatcaga tgggctaact
     1561 catgaaaaat ggaaaatctc tggcgttcga tggtaatggc actattagta ttgataatca
     1621 tcccatactg gttagaatca ttcctcttac cggtcaaagc gaaaagatcg ttattgccta
     1681 aatcggcacc ctggataaaa attccaagat cactaatcag actaacgggg tcttgaatag
     1741 gaacgctacc taaattccaa tttgcgcggg tgacaattgc atggtgttcg ccactgtgac
     1801 gagggtcctg gctttcatcg tatttaagat caggtaattc tcgtataatg atatgttcat
     1861 tagtccgact tttttcacaa gccgagtacc aatcggcaaa ctgttgtctg gtttggtcag
     1921 taatataatg gacattatgc atacgcaaca taaaggcaaa ttcagccggt ttttcaggct
     1981 caaaagcctt taaaataaca ttcggattga tctggcaaat acgaatatat cccaacaact
     2041 ggtcgagatt aatgacctgt cgtccggtct cgatgcgagc gattgtatcc cgagaacaat
     2101 tcatacgtag cgccaaatca ttttgcgtca ggtgcgcttt ttcccgctcc cgacgcagga
     2161 tgctaattag acgttgcatt ttttttactc ctttttcatt gctgacttaa tttaaagcaa
     2221 tacttgatga tatgcaagac cattgtgcga taaacacaca tatattttat ttttacttgc
     2281 aaataacgca attaatagat atattaaggg tagtcgaacg atatttgctt tttgacatta
     2341 tcgaaaaaat aaaaacaagc ccaattgtac tttaacccaa agtttctggt ttttctggct
     2401 gtttttaaca ggaacaaatt aggaaggagg tgagaaccat ggctaaattg attctaccaa
     2461 ggcctgcgct gctcattgcg cagttacaga tgatgtcggc ggcattggca aaatatgccg
     2521 agacttacgt tccagtaagt ccgtcgaaag acgaactcga cgcttgcgtt acggaattaa
     2581 ccagtgcact tcaaaatcag gtacaagcgg ctggtattgc tgaaaaagca actaaagact
     2641 tatatgccgt acgcgatcgg tcagttgatc ttttgcgtcg tatgcgagat gcgctttatg
     2701 cccatttcgg taaatatgac ccacgattgg ttgagttcgg gctcgatact atgaaggcac
     2761 gtaagccgaa aaatggaaat ggttcagatg aaactggtaa tctccagtaa caactgaacc
     2821 attcctcgtc tctgaaacct cccggaactc cagttctggg aggttttgct ttttatacta
     2881 aataactaaa atatcaaaaa tcaattctat ctgaaatata ttggttattt aatctttagt
     2941 caataggaga aaataatact attctcggct ctatatcaaa atatcataac catagtaatg
     3001 cgtaattaag ttaagtaccc tataatttac attatgataa atagccctag cgttgtcaat
     3061 tttaaccaat tggaaaaaca ttttactcac ttatcaattt tgataaacca tttggccatt
     3121 ttgtttgacc ggtaaaatgt ttggctaaat cgtttggcca ttttgtttga ccggtaaaat
     3181 gtttggctaa atcatttggc caatttgttt gactggaaaa atgtttggcc aaatcgtttg
     3241 gccactttgt taaccggtta aaatgtttgg ccaaatcgtt tggcaacttt gtttgactgg
     3301 aaaaatgttt ggccaaatga cttaaatgtt ttattaagtg gattttaaag aataaaaaac
     3361 gaaagatagg attattccgg taggaaattc cgccaaagcg gaatcaatga aagattgaga
     3421 aggcattatc ggctaataat aaagactggg atagggattg cttctcccgc tacggcggga
     3481 tcgttccggc aggaaatccc aaccgtgcgg aatcaatgat acgttgagga tggattattg
     3541 gctaataata aagacaataa agactgggat tgggattgcc acgtcgctac gctcctcgca
     3601 atgacacatt ggggatgggg attgccctcc cgctacggcg ggatcgcaat gacagcttga
     3661 gggattgccc tcccgctacg gcgggatcgg aatgacattc tccccatata ttac
//
LOCUS       DAOSMP010000108         3649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_25450_length_3649_cov_15.366722, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000108 DAOSMP010000000
VERSION     DAOSMP010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25450_length_3649_cov_15.366722"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1889)
                     /locus_tag="PKX09_09930"
     CDS             complement(<1..1889)
                     /locus_tag="PKX09_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU18179.1"
                     /translation="MINFTNQKKDLCLPIIVKQKNLVTFAYKPPKPVREIFVMGNFND
                     WNRSSLPMSDEDSDGIFTRDVWLDDGLYEYQFVVDQNEIYDPANPEKTDNGFGYFNSV
                     LRVTAPLADYAANLYILPTRQNDTITLAVDCLGDTSDIRYFVLLDNHLCPPKSYHHKN
                     KQLQIYLNSLENAIGMHILRATAQYKNQPGNVITIYLKNGKPLDNNTFIWNDACLYAI
                     MTDRFKNGNSGNDRPVVHPHLASQANFQGGDFDGIRRTIESGYFDDLGINTLWISPVN
                     KTTDSAYQEWPEPHNYFSGYHGYWPTDARQTDSRFGSLQELKKLVEVAHRHNIKVLLD
                     FVSNHVHIEHPYFREHREWFCQLELPDGSLNIRRWDEYRLTTWFDTFLPSFDYEGSHE
                     ALQTMTDNAVWWLKQTDADGFRHDATKHVPHAFWQTLTSKINKRINPHRNLDIFQIGE
                     CFGSNELIKSYVNNGMLESQFNFNQFFTARRVFVEPTGDFRDLELAIKKALEVYGYNH
                     LMGNLMDSHDQVRIMAFLDGDLTLSDDGTMRAWQEPPITVDNPLTYRKELVFFGYLLT
                     VPGIPVIDYGDEFGLTGANDPDNRRMMRFGEELSDLEREQLQQVSNLIQLRNMHPALR
                     RGDYLP"
     assembly_gap    1945..2044
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2045..>3649)
                     /gene="pulA"
                     /locus_tag="PKX09_09935"
     CDS             complement(<2045..>3649)
                     /gene="pulA"
                     /locus_tag="PKX09_09935"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HOU18180.1"
                     /translation="EPKKEVLDPYTTCATTHDGRALIFEENTQIAVAPKFLLKDAIIY
                     EMHVRDFTIAENSGVKNKGKYLGFTEEGTTIPGTSISTGLDHLTELGINTIQLLPIQD
                     FEHDNQVNNYFWGYMTVNFNSPDGWYTTNENDESRVREVKQMVSALHQRGIKVVMDVV
                     YNHTAETNPQIRYNFNGIVPSFYYRQREDGSYWNGSGCGNEVRSENPMVRRFIIESLK
                     YWVKTYDIDGFRFDLMGLIGIETMRQIVKELTALKPDIFLYGEPWTVGETPIKPILKG
                     AQREEGFAVFNDNFRDALKGPWHNTDPGYVQTGKNIEAVKIGIRGSIDDFAADPIETI
                     NYVSCHDGRTLWDQLVASTGSNKMYTDTQLKAMDKLAAFIIFTSQGIPFMQGGEEFLR
                     TKFGSNNSYNQPDKINKIRWDYKQENHDIFAYYQGLIRIRRDHPMFRMETAEEVRRNL
                     AFFEELGLSVPPNCIAYRLQRGTSGDIWREVIILINPNSRPETFKIPKAKWILAVDHT
                     YADPEIISPISGPLVTVKPISAMLIYR"
BASE COUNT          945 a          740 c          845 g         1019 t
ORIGIN      
        1 ggtaaataat cgccgcgtcg taaagccgga tgcatattcc tcaattggat taaattgctt
       61 acctgctgta attgttctcg ttccagatca gataattctt caccaaaacg catcatacgg
      121 cgattgtctg gatcgttagc gccagtcaag ccgaattcat ctccgtaatc aataaccgga
      181 attccgggta cagtcagtag atatccaaag aagactaatt ctttccggta agtaagcgga
      241 ttatctactg ttattggcgg ttcttgccag gcgcgcatag ttccgtcatc agacaaggtc
      301 aggtccccat ccaaaaaagc cattatgcgc acctgatcgt gactatccat tagattgccc
      361 ataaggtggt tgtatccata tacttccagt gcctttttaa tagccaattc caaatcgcga
      421 aagtcgccgg tcggttctac gaaaacccga cgagcggtga aaaattgatt aaaattaaat
      481 tgcgattcga gcattccatt attaacataa gatttaatta attcattact gccgaaacat
      541 tcaccgattt gaaaaatatc cagattccgg tgaggattga tccgcttatt aattttggat
      601 gtcagagttt gccagaaagc atggggcaca tgtttagtag catcgtgtcg aaaaccgtcc
      661 gcgtcagttt gctttagcca ccagacagcg ttgtcagtca tagtttgaag agcctcgtgc
      721 gagccttcat aatcaaaaga cggtagaaaa gtatcgaacc aggttgtcag gcgatattca
      781 tcccagcgcc ggatattcag agatccgtcc ggcaactcca attggcaaaa ccactcacga
      841 tgttctctga aatagggatg ttcgatgtgg acatggttgg agacaaaatc aagtaaaact
      901 ttgatattat gacgatgagc cacctcgact aattttttta attcttggag ggagccaaaa
      961 cgtgagtcag tctgtctggc atcagttggc cagtaaccat gataaccgga aaagtaatta
     1021 tggggctcag gccattcctg ataagcacta tcagtggttt tattgaccgg cgaaatccag
     1081 agagtattga tgccgaggtc gtcgaaataa ccggattcga tggtccggcg gatgccgtca
     1141 aaatcgccgc cctggaaatt ggcttgtgat gctaagtggg gatgaactac cggacggtcg
     1201 tttcctgagt tgccgttttt aaagcggtcg gtcattatgg cgtaaagaca agcatcgttc
     1261 caaatgaagg tattattatc gagtggttta ccatttttca aatagatagt gatcacattg
     1321 ccgggttgat ttttgtattg agccgttgcc cgcaaaatat gcatcccgat cgcattttca
     1381 agtgaattta aatagatctg cagttgttta tttttatgat gatagctttt tggtggacaa
     1441 agatgattat ccagcaagac aaaataacgg atatcgctgg tatcaccgag gcagtcaaca
     1501 gctaatgtaa tggtatcgtt ctgtcgtgtc ggcaaaatat agagattggc ggcataatca
     1561 gccagcggag ccgtaacgcg taaaaccgaa ttgaaatagc caaatccatt atcggtcttt
     1621 tccggattgg cgggatcata aatctcgttc tggtcaacaa cgaactggta ttcgtataaa
     1681 ccatcgtcga gccaaacatc ccgtgtaaaa attccgtcac tgtcttcatc agacattggt
     1741 aatgagctcc gattccaatc attaaaatta cccattacga atatttcccg aaccggtttg
     1801 ggaggcttat aggcaaaggt gaccagattc ttttgtttga caatgatcgg cagacaaaga
     1861 tcttttttct ggttggtaaa attaatcata gtcaatccgg aaaatgagtt gttcggagtc
     1921 agaataatta tattggaatc gggtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnncggtaa atcagcattg ccgaaatggg tttgacggta actaagggtc cactaatcgg
     2101 ggaaataatt tccggatcgg cataagtatg atctacggcc agaatccatt tagcttttgg
     2161 gattttaaag gtttccggac ggctgttcgg attgattagg ataataactt cgcgccagat
     2221 gtcgccgctg gtaccgcgct ggagacgata ggctatgcaa ttgggtggta cgctgagtcc
     2281 gagttcttca aaaaatgcca aattgcggcg cacttcttcg gcggtttcca ttcggaacat
     2341 tgggtggtct ctgcggatac ggattagtcc ctgataatag gcgaaaatgt catgattttc
     2401 ctgtttgtaa tcccagcgaa tcttattgat tttgtctggc tggttgtagc tattattgga
     2461 accgaatttg gtgcgcagga attcctcacc gccttgcatg aaaggaatac cctgcgaggt
     2521 gaaaatgata aaagcggcta gcttatccat tgcttttagt tgcgtgtcgg tatacatttt
     2581 attgctgccg gtggaggcaa ctaattgatc ccagagtgtg cggccgtcgt ggcaggaaac
     2641 ataattaatg gtttcgatcg gatcagcagc aaaatcgtcg atactacccc gaatgccgat
     2701 tttaacggct tcgatatttt tgccagtttg aacgtatccc ggatcagtat tatgccacgg
     2761 tcctttgaga gcgtcacgga agttgtcatt aaatacggcg aagccctctt cgcgttgagc
     2821 gcctttcaaa atcggtttga tgggcgtttc accgacagtc cagggttcgc cgtagagaaa
     2881 aatgtcgggc ttaagggcgg ttagttcttt aacgatttgg cgcatagttt ctatgccaat
     2941 taatcccatt aaatcgaagc gaaatccgtc aatgtcataa gtcttgaccc agtatttgag
     3001 tgattcgatg ataaagcgac gtaccatcgg attttcactg cgcacttcat tgccgcatcc
     3061 ggaaccgttc caataagagc cgtcctctct ctggcgatag taaaagctcg gcacaatccc
     3121 attgaaatta taacggattt gcggattggt ttcggcggta tgattgtaaa ctacatccat
     3181 aacgaccttg atgccgcgtt gatgaagggc agaaaccatc tgtttgacct cgcgtacccg
     3241 gctctcgtca ttttcgttgg tggtgtacca accgtccggt gaattgaaat tgaccgtcat
     3301 atagccccag aagtaattgt taacctggtt gtcatgttcg aaatcttgaa tgggtagtag
     3361 ctggatggta ttgattccca gttcagttag atggtccaga ccggtgctga ttgaagtgcc
     3421 gggaatggtt gtgccttctt cggtgaaacc gaggtatttg cctttgttct ttacaccgga
     3481 attttcggcg atagtaaagt cgcggacgtg catttcatag ataattgcat cttttaggag
     3541 gaatttaggt gcaaccgcta tctgagtatt ttcctcgaaa atcaaagccc gtccatcgtg
     3601 agtagtagcg caggtggtat aagggtcgag aacctctttt ttcggttca
//
LOCUS       DAOSMP010000109         3645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_25488_length_3645_cov_18.034262, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000109 DAOSMP010000000
VERSION     DAOSMP010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25488_length_3645_cov_18.034262"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="PKX09_09940"
     CDS             complement(<1..1016)
                     /locus_tag="PKX09_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOU18181.1"
                     /translation="MSRQRKLLELKDKIELARMGGGAEAIEKQHQRGKLTARERLQLL
                     LDPNSFEEIDAFRTHSCTDFGMAEKKFLGDGVVTGYGTINGRLVFVYSFDFTVFGGSL
                     SKVVAEKIVKIMEMATKVGAPLIGINDSGGARIQEGVDSLAGYTEIFVRNVLYSGVIP
                     QISAIVGPCAGGAVYSPALTDFIFMVRKSAYMFLTGPKIVKTVTHEDVTADELGGADV
                     HASKSGITHFTYDDEQSLFAGMRELLSYLPQNNMEEPPAIAPTDPVDRIDEDLNYIVP
                     ENPNKPYDMKEILAKVLDDGQFLEVQALYATNMLIGFGRLNGKVVGIVANQPRSLAGV
                     IDTNA"
     gene            complement(1107..1556)
                     /locus_tag="PKX09_09945"
     CDS             complement(1107..1556)
                     /locus_tag="PKX09_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOU18182.1"
                     /translation="MDNKIHEELYKDKELTSTIIGCAYKVYKNLGFGFLESVYEKALI
                     IEMTKAGLKSESQKAIDVYYDDQIVGHFIADLFVEGKIIVELKSVATLTKSHEVQLVN
                     YLTATRINIGLLINFGEEKVEIKRKIRSLKQSNQSCKSCQKKKSQSC"
     gene            complement(1611..2015)
                     /gene="mce"
                     /locus_tag="PKX09_09950"
     CDS             complement(1611..2015)
                     /gene="mce"
                     /locus_tag="PKX09_09950"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOU18183.1"
                     /translation="MIKKLNHIGIAVNSIAEHIPFYRDILQLKFVGIEEIADQKVKTA
                     IFQVGETRIELLEATAPDSPIAQFLEKRGEGLHHLAYQSDNIERELQFLKEHNIALID
                     EKPRDGAHHTRIAFLHPKSTGKVLTEICEEKR"
     gene            complement(2020..2961)
                     /gene="meaB"
                     /locus_tag="PKX09_09955"
     CDS             complement(2020..2961)
                     /gene="meaB"
                     /locus_tag="PKX09_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOU18184.1"
                     /translation="MAEKILKGDQRSIGRAISIVENDGRDREQLIDEIFPHTGRALVW
                     GITGAPGSGKSTLLDGLIANLRAQNRTVAVVAVDPSSPFTGGALLGDRLRMQRHATDL
                     GVFIRSMASRGHLGGIATATADTIKILDAAGYDLIVIETIGVGQAEIEVVELADIVML
                     VLVPGAGDEIQAMKAGVMEIGDIFIINKKDLDGALKLKAEVEYVLHLKSNSNESAINP
                     VLLTSATHNEGISETLQTIDNYLARMQADGRLAIRRQQRIENEVRKIVNTKIHQMAEK
                     HLQIDQKIKQWAQQIGKQKQAPYALINSQINQLLKDS"
     gene            complement(2967..3371)
                     /locus_tag="PKX09_09960"
     CDS             complement(2967..3371)
                     /locus_tag="PKX09_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOU18185.1"
                     /translation="MAKKIRILIAKPGLDGHDRGAKYIARALKDAGYEVIYTGIRQTP
                     EAIVKTAIQEDVDFVGLSLLSGAHNELFPRVVELLGENQADDIIVFGGGVIPESDIPG
                     LKEKGLAAIFTPGTPIKAVIDFIESCRSKIEN"
     gene            complement(3381..>3645)
                     /locus_tag="PKX09_09965"
     CDS             complement(3381..>3645)
                     /locus_tag="PKX09_09965"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOU18186.1"
                     /translation="DEETPKNLLKVDSELQRIQIQKTNAVKKQRNNQAVKEKLELLRE
                     TARRTEQNLMPPILAAVKEYATLGEICGILRQEFGEYQESIVL"
BASE COUNT          903 a          816 c          801 g         1125 t
ORIGIN      
        1 gcgttagtgt caatgacgcc cgccagcgat cggggttggt tggcgacaat gccgacgact
       61 ttgccgttta aacgtccgaa cccgattagc atattggtgg cgtacaaagc ctgaacttcg
      121 aggaattgtc cgtcatccag aactttggcg agaatttcct tcatatcgta aggcttattg
      181 ggattttcgg gcacgatata attcaagtcc tcatcaatgc gatcaaccgg atcagtcggt
      241 gcaatggcgg gcggctcttc catattgttt tgcggtaaat agctcagcag ttcgcgcata
      301 ccggcgaaca gactttgttc atcgtcatag gtaaagtgag taatgccgct tttgctggcg
      361 tgaacatcgg caccacccag ttcatcggcg gtgacatcct cgtgagtaac agttttgacg
      421 attttcgggc cagtcaggaa catataggct gactttctaa ccataaaaat aaaatcggtc
      481 agcgccggtg aataaactgc tccgccggcg caaggtccga cgatggcgct aatctgggga
      541 atgacgcccg agtaaagcac attgcgcaca aaaatttcgg tataaccggc cagtgaatcc
      601 accccttcct gaattcgcgc gcctccggag tcattgatgc cgattaacgg agcaccgact
      661 ttagtcgcca tttccataat tttgacaatt ttttcagcta cgacttttga tagtgaacca
      721 ccgaagaccg taaaatcaaa agaataaacg aataccagac gaccattgat agtaccataa
      781 ccggtgacga ccccatcgcc gagaaatttc ttttcagcca taccgaagtc ggtgcaggaa
      841 tgagtgcgaa aggcatctat ttcttcaaaa ctattcggat cgagtagtaa ttgcaggcgc
      901 tcccgggccg ttaacttgcc gcgctgatgc tgtttttcga tggcttccgc accgccgccc
      961 attctggcaa gttcaatttt atcttttaat tcaagcagtt tacgttgacg actcatttat
     1021 gctccttgta tattttattg acaagattaa cattgttttt attttagaca agattaacaa
     1081 gattgatttt ttttctttag acaagattaa caagattgac tttttttctt ttgacaagat
     1141 ttacatgatt gatttgattg ttttaatgat cttattttgc gtttgatttc gactttctcc
     1201 tctccgaaat ttattaatag tccgatattt attctggtag ctgttaagta attcactaat
     1261 tgaacttcgt gacttttcgt caaagtcgct acagatttta attcaacgat tatttttcct
     1321 tctacaaaaa gatcggcaat aaaatgtcca acaatttggt catcataata gacatcaatt
     1381 gctttctgag attcggattt caaacccgct ttggtcattt caattatcag agccttttca
     1441 tagacggact ctaaaaagcc gaatcccagg tttttataga ccttgtaagc acaaccgatt
     1501 atggtggatg tcaattcttt atccttgtat aactcttcgt gtattttatt atccatctta
     1561 gttctaaata ttaataaaca acatgtaaat cttgttaatc ttgtctaaga ctacctcttt
     1621 tcctcacaga tttcagtcaa gaccttgcca gtggatttcg ggtgcaggaa agcgatgcga
     1681 gtatgatgtg cgccgtctct cggcttttcg tcaatcaagg caatattatg ttcttttaaa
     1741 aattgtagtt ctcgttcgat gttgtcgctt tggtaagcca gatgatgcaa accttcgcct
     1801 cttttctcga gaaattgggc gattggacta tcgggcgcgg tagcttcgag taattcgatc
     1861 cgggtttcac cgacctgaaa gatggcggtt ttgactttct gatcggcaat ttcttcaatg
     1921 ccgacgaact ttaattgcaa aatatcccga taaaacggaa tgtgttcagc gattgaattg
     1981 accgcgatgc cgatgtggtt gagttttttt atcatagaat taggagtcct ttaataattg
     2041 attaatttga gaattgatca aggcataagg cgcttgcttt tgttttccga tttgctgagc
     2101 ccattgcttg attttttggt caatttgcag atgtttttca gccatttggt ggattttggt
     2161 gttgacgatt tttctgactt cgttttcgat ccgctgttga cggcgaattg ccagccgacc
     2221 gtcagcctgc attcgggcaa ggtaattgtc aatagtttgg agtgtttccg atatgccttc
     2281 attatgagtt gccgaagtta atagaaccgg atttattgcg gattcattac tatttgattt
     2341 gaggtggagg acatactcga cttcggcttt gagtttgaga gcgccgtcga ggtctttctt
     2401 attgataatg aaaatatcac cgatttccat cactccggct ttcatcgcct gaatttcatc
     2461 acccgctccc ggtaccagaa cgagcataac gatatcagcc agttcgacga cttcgatttc
     2521 agcttgcccg acaccaatgg tctcaatcac gattaaatca tagccggcgg catccaaaat
     2581 tttaatagtg tcagccgtgg cagtggcaat gccacctaaa tgtccgcgcg aagccatcga
     2641 gcggatgaaa acgcctaaat cggtggcatg tctttgcatg cgtaaacgat caccgagaag
     2701 cgctccgcca gtaaacggtg aactgggatc aaccgcgact accgccaccg tccggttttg
     2761 agcccgcaaa ttggcgataa gaccatccag caaagtgctt tttccactgc cgggtgcgcc
     2821 ggtgattccc cagactaatg cccggccagt gtgcgggaaa atttcgtcaa ttaattgttc
     2881 gcggtccctg ccgtcatttt ctacaataga aatggcacga ccgatgctcc gctggtcgcc
     2941 tttcaaaatt ttttcagcca attcgctcaa ttttcgattt tgctccggca ggattcaata
     3001 aaatctatga cagctttaat tggcgtcccg ggtgtaaaaa tggcggctaa tcctttttcc
     3061 tttaaaccgg gaatatcact ttctgggatg acgccaccgc cgaaaacaat gatgtcgtca
     3121 gcctgatttt cacctaaaag ttcgacaact cgggggaaaa gctcattgtg agccccggat
     3181 aacaggctga gcccgacgaa gtcaacatct tcctgaatag cagttttgac aatggcttct
     3241 ggtgtctgac gaattccggt gtagattacc tcatatccgg catctttcag agcgcgggcg
     3301 atgtacttgg cgccgcggtc gtgtccatcc agcccgggtt tggcaattaa tattctgatc
     3361 tttttagcca tattgaatcc ttataaaacg attgattcct ggtattcgcc gaattcctgg
     3421 cgcagaatgc cacagatttc gcccagtgtg gcatattcct taacggcggc gagaatggga
     3481 ggcataagat tctgctccgt tcggcgcgca gtttctcgta aaagttcaag tttttcctta
     3541 actgcttgat tgttgcgttg ttttttgaca gcgttagttt tttggatttg gatgcgctga
     3601 agttcggaat caacttttaa taaatttttc ggtgtttctt cgtcg
//
LOCUS       DAOSMP010000110         3605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_25883_length_3605_cov_20.066761, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000110 DAOSMP010000000
VERSION     DAOSMP010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25883_length_3605_cov_20.066761"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PKX09_09970"
     CDS             complement(<1..534)
                     /locus_tag="PKX09_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU18187.1"
                     /translation="MNLLDNLTWLGHSSFRIAGKKIIFIDPFQVKVGTPADLILITHD
                     HYDHFDKNSISNIKKNDTIIVAPASVCRQISGDTRPVRPGAQMNFDSVTIKAVPAYNI
                     GKKFHPRENQFVGYIIHADNIIYYHAGDTDLIPEMQDIRADVALLPIGGTYTMDVIEA
                     AQATKIIHPQVAVPMHWG"
     gene            complement(553..1332)
                     /locus_tag="PKX09_09975"
     CDS             complement(553..1332)
                     /locus_tag="PKX09_09975"
                     /inference="COORDINATES: protein motif:HMM:NF019003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HOU18188.1"
                     /translation="MRKTDFSPLPERKQLLYEQMARSYRTQERQKNTPWAPFEKRLIE
                     SKIAVISIAGAYLNGQKPFTNRETESDYQCREIPLNFNREDLRFFATDWEPSEALADY
                     NVVLPIDRLVLLQKEGMIGKIHETIISIAGFTKSIKMFGKFLTKTYDTLKKAEVDGVL
                     ILPCSAQTSAAACQVANYLEKKELPTVILTTFYEQALALAPPRCAFINFPFGRPFGKA
                     NHVTLHTAILRDTLRLFEKAKTPGEVICLSYIWSFGTVPNW"
     gene            complement(1383..2111)
                     /locus_tag="PKX09_09980"
     CDS             complement(1383..2111)
                     /locus_tag="PKX09_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOU18189.1"
                     /translation="MQNLIYVGRWLVGLVYFLLLGITLRFGLIFWKPAEIYVLVQQIC
                     RAALRTIGIRIKVSGLDRFDHQQSYLIICNHESLLDAFICPAYIPMYFTTIELADHFK
                     WPLWGWMIRNWGNIPLQHGQLKSSLTSLDQAATLLKNGTSILIFPEGSRTTDGKLLSF
                     KKGAFYLAYRAKADILPLAINGLWRAKTRGDWRLRSANVSLNFGEPIRYSTYQHLSPD
                     ELRNLCFETIQQLKSPVASNVLSS"
     gene            2302..>3605
                     /locus_tag="PKX09_09985"
     CDS             2302..>3605
                     /locus_tag="PKX09_09985"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOU18190.1"
                     /translation="MKIRNIIAVLGSLFTILGLFLILPLLISLFQHDAATPAFLFSIA
                     VATGVGLLMQIRKPRNFNIQRWEAMVIVVIGWIGASVIGALPFYFSGYFDGFINALFE
                     SVSGFTTTGASILNDIESLPISLLVWRTLTHWIGGMGIIILALVVLPAINISGRNLYS
                     FESSSLANEQITPKIKDTARLLWTVYFILTVSETVLLLILGLPFFDAALTAMATIATG
                     GFSPKNASIAAYNNVYVEIVVIIFMILSGINFTLYLRMFQSRKNWRDYSETVYIYFAI
                     MAVASLIIVINLRSHTYNSWSEAIRYGVFQAVSINTTTGFVTTDWDIWQPVAHIVLLI
                     LMLVGACPGSTCGAIKNSRIVLLIKSIHRELIQIIHPRVVTTIHLNKRPIDEKTIQNA
                     IVYIGMYLCFWLVASMIVALGGVAPESALIAVATTLGGVGPG"
BASE COUNT          966 a          756 c          841 g         1042 t
ORIGIN      
        1 accccagtgc atcggtacgg cgacctgcgg gtggattatt ttggtggctt gggcggcttc
       61 gatgacatcc atcgtataag tcccaccaat cggcagaaga gctacatcgg cgcgaatatc
      121 ttgcatctcg ggaatcaagt cggtatctcc tgcatgataa taaataatgt tgtcggcgtg
      181 tattatgtaa ccgacgaact gattttcgcg ggggtgaaat tttttaccaa tgttataggc
      241 ggggacagct tttatagtaa ccgaatcgaa gttcatctgc gcacctggtc ggaccggtcg
      301 ggtgtcgccg ctaatttgtc ggcaaacact ggcaggcgca acaattatgg tgtcgttttt
      361 cttaatattc gaaattgaat ttttatcaaa atgatcgtag tgatcgtgcg taatgaggat
      421 aaggtcagcc ggtgtcccga ctttaacctg aaagggatca ataaagatta tcttttttcc
      481 ggcgatccgg aaagatgaat gtcctagcca agttagattg tccagtaaat tcataaatca
      541 tctccaaaag tattaccaat tcggcactgt tccgaatgac cagatataac ttagacagat
      601 cacttcgcct ggagttttgg ctttttcaaa aagacggagg gtgtctcgca gaatcgccgt
      661 atgcaaggta acatgattgg ctttgccaaa cgggcgacca aaaggaaaat tgataaaagc
      721 acagcgggga ggcgccaagg ctaaagcctg ttcgtagaag gtggtcagta taactgtcgg
      781 cagttctttt ttttccaaat aattagcaac ctggcaagcg gcggcagatg tctgagctga
      841 acaaggcaga attaagacgc catcgacctc ggcttttttc agggtatcgt aagttttggt
      901 caggaatttc ccgaacattt ttatactctt ggtaaaaccg gcgatactga tgattgtctc
      961 atgtattttg cctatcatac cctctttttg taaaagcacg agacggtcaa tcggcaagac
     1021 gacattataa tcagccagag cttcgctggg ttcccaatca gtggcgaaaa aacgtaggtc
     1081 ttcgcgatta aaattgagag gaatctcgcg gcattggtag tcgctctcag tctcgcgatt
     1141 agtaaatggc ttctggccgt tgagataagc gccagcaatc gaaattacgg caattttact
     1201 ttcgattaat cgtttctcaa aaggggccca cggcgtattt ttctgccgtt cttgagttcg
     1261 ataactacgc gccatctgtt cgtaaagcag ttgtttacgc tcaggtaatg gggaaaagtc
     1321 tgtttttctc atagtggtaa tataagaaat tttatgttaa tctcaagtcg tatttaagac
     1381 ttttaagacg ataaaacatt cgatgctacc ggactcttca attgctggat tgtttcaaaa
     1441 cagagattgc gcagttcatc gggcgaaagg tgttggtagg tagagtagcg aatcggctca
     1501 ccgaaattca ggctgacatt ggcggaacgt aagcgccaat cgccacgagt tttagcccgc
     1561 cagagcccgt taatagccag cggcaaaatg tctgccttag cgcgatatgc cagataaaaa
     1621 gcaccttttt taaaggaaag caatttacca tcggtagtgc ggctaccttc gggaaaaatc
     1681 agaatcgagg tgccattctt aagcaaggta gcggcttggt ctaaacttgt tagggaggat
     1741 ttgagctgtc cgtgttggag cgggatattc ccccaattac ggatcatcca gccccagagt
     1801 ggccacttga aatggtcggc taactcgata gtagtaaaat acatcgggat ataagccggg
     1861 cagatgaaag catccaggag actctcatga ttacaaataa tcaagtaaga ttgctgatga
     1921 tcaaagcgat ccaaaccact gacttttatg cggatgccga tcgttcgtag agcggcgcgg
     1981 cagatctgct gtaccagaac atagatttcg gcaggtttcc aaaaaataag cccgaaccgg
     2041 agagttattc ctaacaacag aaagtaaacc aacccgacca gccagcgccc gacgtaaatc
     2101 agattttgca ttttcgaatg ataatatcct cttgattcat agaccaaatc tggtaatttt
     2161 caatactttt tacaacgtaa tttttatcta ttcgcttatc aaataaatta ggaaatttgc
     2221 aatctaatca taaaagaggt aaaatattgg gcattatgat ctctatgttg aaatgctcat
     2281 aattagggag cagttacagt tatgaaaatt cgcaacatta tcgcggttct gggtagtctt
     2341 tttaccatat taggattgtt cctgattttg ccgttgctga ttagcctatt ccaacatgac
     2401 gcggccactc cggcatttct tttcagtatt gcagtcgcta cgggcgtcgg attattaatg
     2461 cagattcgca agccccgcaa ttttaatatt cagcgctggg aggcaatggt aatcgtggtt
     2521 atcggatgga tcggggcatc tgtgatcggc gcattgccgt tttatttcag cggatatttc
     2581 gatggtttta taaacgctct gtttgagtcg gtttcgggat tcaccaccac cggcgcttcg
     2641 attctaaatg atattgaaag cctaccgatt agtctgcttg tctggcgcac tctgacgcac
     2701 tggatcggtg gtatgggaat cataatcctg gcgttagtag tgcttccagc tattaatatt
     2761 agcggacgca atttgtacag cttcgaatcc tcttcgcttg ccaatgaaca aatcacgcct
     2821 aaaatcaaag ataccgcgcg tttgttatgg acggtgtatt ttatcctgac agtctccgaa
     2881 accgttcttt tattaatttt aggtctgccc tttttcgatg cggcattaac ggctatggcg
     2941 actatcgcca cgggcgggtt ttcacccaaa aacgccagca tcgccgccta taataatgta
     3001 tatgttgaaa tagtcgttat tatttttatg atactctccg gcataaattt cactctctac
     3061 ctacgaatgt tccaatcacg aaagaactgg cgggattact cagaaacagt ctatatctat
     3121 tttgcgatta tggcggtcgc atctttgatc atagtgatta atttgcgttc gcatacttac
     3181 aattcctggt cagaagcaat tcgctatgga gtatttcagg cagtttcaat taatacgacg
     3241 actggattcg ttacaactga ttgggatatc tggcaaccgg tggctcatat tgttttgtta
     3301 attttaatgt tggtcggggc ttgtccggga tccacgtgcg gagctataaa aaattcgcga
     3361 atcgttctgc ttatcaaaag catccatcgc gagctcattc agataatcca tccgcgggta
     3421 gtcacgacta ttcatttgaa taaacgccca attgatgaaa aaacaatcca gaatgcgatt
     3481 gtttatattg gaatgtatct gtgtttttgg ttggtcgcga gtatgatcgt ggctttaggc
     3541 ggagttgccc cggaatcagc gctcattgcc gtggctacaa ccctgggagg cgttggacca
     3601 ggcat
//
LOCUS       DAOSMP010000111         3487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_27051_length_3487_cov_6.643065, whole genome shotgun sequence.
ACCESSION   DAOSMP010000111 DAOSMP010000000
VERSION     DAOSMP010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27051_length_3487_cov_6.643065"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PKX09_09990"
     CDS             <1..487
                     /locus_tag="PKX09_09990"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N,N'-diacetylchitobiose phosphorylase"
                     /protein_id="HOU18191.1"
                     /translation="FSHTQGWAIIAETMLGHGNRAYQYLRAYLPAAYNTRAEIRQIEP
                     YVYCQFTNSRYSPRPGASRLPWLTGAAAWSYYTTTQYILGIQPDYFGLRIDPCLPSNW
                     KEVRIKRRFRNKMFNILIKNPNGAQKGIRKLIINNQELTGNLIPLDIIQNENKVVVEM
                     Q"
     gene            560..1600
                     /locus_tag="PKX09_09995"
     CDS             560..1600
                     /locus_tag="PKX09_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18192.1"
                     /translation="MKKILQRFFLICPKTGKVRGIKPLSGWSKLLFPITGLLALIWVV
                     VRVLPKPTRATYPCMRVAMPLASGFIVYLIGVIASATAFMKAKKMLAKSRYILATGFV
                     ILGLLASLLIFHNSRETAYATYATIEHESNIPIGTARGIFPGRVVWVYNPDATNENCV
                     PDSTVSSWWGGSSYTTYRAGRAWFLPQNNNQAVVDSMVSSAIQSLTGETSDSAAWVAI
                     FKYHNTTREKGAVNYSPGEKIFIKINATSGWGGNYSTTDLSCAKNSSYGISETSPAVV
                     MTVLRHLVNIVGVKQGDIYIGDPMKHIYKHLYDYWHSEFDSVHYLDHDANYANLGREQ
                     GADRHNDQDLLF"
     gene            1554..2660
                     /locus_tag="PKX09_10000"
     CDS             1554..2660
                     /locus_tag="PKX09_10000"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOU18193.1"
                     /translation="MNRAQIGTTTKIYYSDEGTVLRENVWDANRPGEGPVYDDNVYAI
                     LEDAEYLINIPMLKGHRRGGITMFAKNHFGSHTRPDAGHLHNGLVAPREMELGVTRPG
                     YRQYRVQVDIMSHSILGDKNLLYLMDALWATDYELDKPLKWQMAPFNNDFTSSIFASF
                     DPVAIESVGYDFLRSEFTAERVPSAGTYVQMDGVDDYLHQAADSANWPEGIVYDPDND
                     GILFASLGVHEHWNNATEMQYSRNLDPESGIGIELVTITRSVSNAIEAEPANLISAFS
                     LYPNYPNPFNATTTIRYALKAPAHVELKVYNLMGQLVSVLENNYKTAGEYVAVWNGET
                     ITGQKATSGVYVYQLNMAVGRQVAVESRKMLLVK"
     gene            2673..>3487
                     /locus_tag="PKX09_10005"
     CDS             2673..>3487
                     /locus_tag="PKX09_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015235670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU18194.1"
                     /translation="MKVLFIGGTGIISSACSQMALERGIDLYLLNRGQCFRSIPKGAH
                     HLQADIRERESVKAAIGKMNFDSVVEWLAYTPDQIESDLESFKGRTEQYVFISSASAY
                     QKPPEKLPITEETPLDNPYWEYSRNKIACEKRLLKAFQEEQFPITIVRPSHTYDCTSL
                     PMEGRYTVVNRMRQGKKVIVHGDGSSLWVLTHHRDFARGFLGLLGNPKTIGESFHITS
                     DELLTWNQIFEIIAEAAGTPAQIVHVPSEVIARYDTEWGDSLLGDKMHSVIFD"
BASE COUNT          977 a          720 c          809 g          981 t
ORIGIN      
        1 tttctcgcat actcaaggtt gggcgataat cgctgaaact atgttgggac acggcaatcg
       61 agcttaccag tatttacggg cttatctacc ggcggcttat aataccagag ctgaaatccg
      121 gcaaattgaa ccttatgttt attgtcagtt tacgaatagt cgctatagtc cacgcccggg
      181 ggcttcacgt ttgccgtggc tcaccggagc cgccgcctgg tcgtattata ctaccactca
      241 gtatattctc ggcattcagc cggattattt cggtttgcgg attgatccat gcctcccttc
      301 aaactggaaa gaagtgagga ttaaaaggcg ttttcggaat aaaatgttta atattttaat
      361 aaaaaacccg aacggtgctc aaaaaggcat tcgaaaacta atcataaaca atcaagaatt
      421 aaccggaaat ctaataccgc tggatataat tcagaacgaa aataaagttg ttgtagaaat
      481 gcaataaaat ttttatttta atgataaatg cagaatgaaa ttatttgata atcactaaca
      541 atctgaaaga gagaaactga tgaagaagat cctgcaacga ttctttttaa tttgtccgaa
      601 aaccggcaaa gtcagaggaa ttaaaccgct tagtggttgg tcgaagttat tatttccaat
      661 aactggtttg ttagcattaa tctgggtagt agttcgtgtg cttcccaaac cgactcgtgc
      721 aacttatcct tgtatgcgcg tcgctatgcc gctcgcttcg ggatttatcg tttatttaat
      781 cggggtaatc gcttcggcta cagcttttat gaaagccaaa aaaatgttag ccaaatcgcg
      841 ctacatccta gcgactggat tcgttattct gggattattg gcttccttgc tcatctttca
      901 taattcccga gagacagctt acgctactta tgcaactatt gaacacgaat ccaatatacc
      961 gatcggaacg gctagaggta ttttccccgg tcgggttgtc tgggtttata atcctgatgc
     1021 taccaatgaa aattgcgtac cggattcaac tgtgagttcg tggtggggtg gtagtagtta
     1081 tactacatat cgtgccgggc gggcgtggtt tttgccccag aataataatc aagcagtggt
     1141 tgacagtatg gtttcttcag caatccagtc tttaaccggc gagaccagcg atagtgccgc
     1201 ctgggttgcg atattcaaat atcacaatac aactcgggaa aaaggcgcag ttaattattc
     1261 acctggtgag aaaattttca tcaagataaa tgccaccagc ggctggggcg gtaattacag
     1321 cacgaccgat ttatcatgcg caaaaaactc tagttacgga atttccgaga cttcaccggc
     1381 ggtagtgatg acggttttac gccatttggt aaatatagtc ggagttaaac agggtgatat
     1441 ttatatcggt gatccgatga aacacatcta taaacatcta tacgattact ggcatagcga
     1501 atttgacagt gttcattatc tggatcacga tgccaattat gctaatttag gtcgtgaaca
     1561 gggcgcagat cggcacaacg accaagattt attattctga cgaaggaacg gttctacgag
     1621 aaaatgtctg ggatgccaac cggccgggag aaggtcccgt ttatgacgat aatgtttacg
     1681 ctattctcga agatgcagaa tatttgatta atatacctat gttaaaaggt caccggcgcg
     1741 gcggaataac catgttcgcc aagaaccact tcggatcgca taccagaccc gatgccggac
     1801 atctgcacaa tggattggta gcgccgcgtg aaatggaact gggagttacc cgtccaggat
     1861 accgtcaata tcgggtgcaa gttgacatta tgagtcactc gattttgggc gataagaact
     1921 tgttatattt aatggatgcc ttatgggcaa ctgactatga acttgataaa ccgctcaagt
     1981 ggcagatggc gccgttcaat aatgacttta cctcgtctat ttttgcttct tttgatcctg
     2041 tggcgattga gtctgtcggt tatgattttc tgcgttcaga attcactgcc gagcgagttc
     2101 cttccgctgg cacttatgtg cagatggatg gcgtggatga ttacttgcat caagcggctg
     2161 attccgcaaa ttggccagaa ggtatcgttt atgacccgga taatgatgga attttgtttg
     2221 ccagcctggg tgttcatgaa cactggaata atgccactga gatgcaatac tcacgtaatc
     2281 tcgatcccga atccggtatc ggaattgaat tagtgaccat tacgcggagc gtttcgaatg
     2341 cgatcgaggc tgaaccggct aatctcatca gcgctttcag cctttatccg aattatccga
     2401 atcccttcaa tgctaccact actatccgct atgctctaaa agcgccggcg catgttgaat
     2461 taaaagtata taatttgatg gggcaattgg tttccgttct ggagaataat tataaaactg
     2521 ccggtgagta tgtggcagtc tggaatggag aaacgattac cggtcaaaaa gctacctcgg
     2581 gtgtttatgt ctatcagcta aatatggcag taggtaggca agtagccgta gaatcacgta
     2641 aaatgctgtt agtcaaatag agatagttat ttatgaaggt tttatttatc ggcggaactg
     2701 gtattatcag ttccgcctgt tcacagatgg cgctggaacg cggtatcgat ctatatctgc
     2761 tcaatcgcgg ccaatgtttt cggtcaatcc ctaaaggtgc gcatcatctt caggctgata
     2821 ttcgcgagag agaatcagtc aaagccgcaa tcggtaagat gaatttcgat tcagttgtgg
     2881 agtggctagc ctatacaccg gatcaaatcg aatccgattt agaatcattt aagggacgca
     2941 ccgaacaata tgtatttatc agttctgctt ctgcttatca aaaaccgcct gagaaattgc
     3001 caattacgga agaaactccg cttgataatc cgtactggga atattcacgt aataaaattg
     3061 cctgtgaaaa gcgtttgcta aaggcttttc aagaagagca gtttccaatt acaattgtca
     3121 gaccatcaca cacttacgat tgcacaagtt tgccgatgga aggcagatac accgtcgtca
     3181 atcgcatgcg tcaggggaag aaagtcatcg tccatggcga tggcagttcg ctctgggttc
     3241 tgacgcatca tcgcgatttt gccagaggat ttctcggttt attaggaaat ccaaaaacaa
     3301 tcggcgaaag ttttcatatt acctccgatg aattgctgac ttggaatcaa atttttgaga
     3361 taattgccga agcggctggc acgccagcgc agattgttca cgtaccgtcg gaagtcattg
     3421 ccagatatga caccgaatgg ggcgatagcc tgctgggcga taaaatgcac agtgtcattt
     3481 tcgataa
//
LOCUS       DAOSMP010000112         3482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_27100_length_3482_cov_14.722790, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000112 DAOSMP010000000
VERSION     DAOSMP010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27100_length_3482_cov_14.722790"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="PKX09_10010"
     CDS             <1..1317
                     /locus_tag="PKX09_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HOU18195.1"
                     /translation="PLDFHLIFPEWYEPDLRAMVRRDRNCPSVIIWSIGNEVGEQYTD
                     EAGAALAKHLCEIVKEEDPKRPATSAMNWAKPYMPFPAVLDLISLNYQGEGIRQEPEF
                     EGTDRIRTAPQYDAFHEKFPDKVILSSETASTLSSRGIYLFPVTEKNSDFVRDGRGGD
                     SKIRQVTAYELHAVDFGASPDKVFASLARHPFVAGEFVWTGWDYLGEPTPYYDARSSY
                     SGIIDLAGFKKDRFYLYQSQWRPDLPMAHILPHWNWPERAGLVTPVHVFTSGDEAELF
                     LNGKSLGRKKKGQFEYRLRWDDVKYQPGELKVVAYKNGEKWAEDIVRTTGEPHRLEAI
                     ADRSEIQADGKDLSFITIRVVDRNGLSVPQATNLITFKIEGCGEIVATDNGDPTDFTP
                     FPSHERKAFSGLALVIVRSKPGQSGTITVTAESLGLKKARAFITSK"
     gene            1396..>3482
                     /locus_tag="PKX09_10015"
     CDS             1396..>3482
                     /locus_tag="PKX09_10015"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOU18196.1"
                     /translation="MSFIFGQSPSFQTYINPVIPGDHPDATLTRIGNDFYTTGSSFNV
                     TPVIYHSTDLVHWQAIAQPVSAAWSNYGDQAGGGCWGGQMVYYNGEYWHFFSRQNTMY
                     YVKASAPEGPWSTPVKINNPSTLPYGLGYDNSIFIDDDHKWYLVVKNGQPNNGIVELG
                     NDGQPTGVVYNLNWLNPGPDYPYSWAEGPVMWKYNGYYYYSFARDLGGGQKVMRSRTL
                     TADQSAWEMLGDFFNENDPQKGSSLFTSPNHASAAVMLDDSTSWVIHPLYAKGEWKGQ
                     GRQGLLNQVHYDANGKPVADYPVNKPFTAPKLPSSGIPWMVPHSDFFTSSELHPEWSF
                     LGYTPAVKYSLTDRPGWLRLSPKNKALANTVIKNDGEHNYSLITRLDFNAQSTTDQAG
                     LWIMRGDQTMEVKLVSTLSTEGLKVITYSFKSTIYETENTIGDTLWLKLVRINHKISG
                     YFSGDGFDWIEIGSPIDISEIDSYSDFSTFTGTRQGLYVKGMSDAWFDFYIYRDAYTP
                     IMAECPANQFGTALVWNKYPPNSLGKIHNDDWALYAGVEFGNTEYAKNCDSLKITASC
                     LIAGSAVEVYLDSIDVNTKIAVCAIDTTGSWTNFRTFTTDLLKPISGNHDVYLKFVGP
                     GSGELFRLQSFQFITNSKPTAINSRPENNLPRSFYLGQNYPNPFNPVTRIKYSVPKAG
                     YLTIKVYDVMGREV"
BASE COUNT          942 a          772 c          819 g          949 t
ORIGIN      
        1 ccgttagact ttcatcttat ttttccagaa tggtatgagc cggatttgcg cgccatggtg
       61 cggcgtgatc gcaattgtcc gtcagtgata atctggagta tcggcaatga agtcggtgaa
      121 caatatactg atgaggcggg cgctgcactg gcaaaacatt tatgtgagat tgtcaaggaa
      181 gaagatccta agcgtccggc gacaagcgcg atgaactggg cgaaaccgta tatgccgttt
      241 ccggcggtac ttgatctcat tagcctgaat taccagggcg aaggaattcg tcaggagccg
      301 gaattcgaag gcactgaccg tattcgcacg gcaccccaat atgatgcttt ccacgagaaa
      361 tttccagata aagtcattct cagtagcgaa acggcttcga ctctcagtag tcggggcatt
      421 tacctttttc ctgttacaga aaagaatagc gatttcgtcc gggatgggag aggcggcgat
      481 tcgaaaattc ggcaagtaac agcttacgaa ttacacgccg tggactttgg cgcctcaccg
      541 gataaagtat ttgcttcgct ggctcggcat ccctttgtag cgggcgaatt tgtctggact
      601 ggctgggatt atctcggtga gcccacgcct tattacgacg cccgcagttc ttattcggga
      661 atcattgatt tggctggatt taaaaaagat cgcttctatc tctatcaatc ccaatggcgt
      721 cccgatttac cgatggcaca tatcctaccg cattggaatt ggccggaacg agccggtctg
      781 gttacgccgg tgcatgtttt tacttccggc gatgaagcgg aattatttct aaatggcaaa
      841 tcgctaggca ggaagaaaaa agggcagttt gagtatcgtc tccgctggga tgatgtgaaa
      901 taccaacccg gcgagcttaa agtcgtcgcc tataaaaacg gtgaaaaatg ggcggaggat
      961 attgtccgaa cgactggtga accgcataga cttgaggcaa ttgccgatcg cagtgaaatt
     1021 caagccgacg gaaaagattt gtcatttatt accattcggg tagttgatcg aaacgggctt
     1081 agcgttccgc aggcaactaa tttaattacc tttaaaattg aaggatgcgg cgagattgtc
     1141 gccaccgata acggtgatcc aactgatttc acaccattcc cttcgcatga aagaaaagct
     1201 ttcagtggtt tagctctagt gatcgttcgc tctaaaccag gccagtctgg taccataacg
     1261 gttaccgccg aatcgttagg attgaaaaaa gccagggcat ttatcacgag taaataatta
     1321 gataatcaaa gaactaaatt aaatgtgagg acttcaatgc gatttttacc taaaatatta
     1381 atttgtttaa ttccaatgag ttttattttt ggtcagtcgc cgtctttcca gacctatata
     1441 aatccggtaa taccaggtga ccatcccgat gctactttga cccgcatcgg caatgatttt
     1501 tataccaccg gttcgtcatt taatgtaact ccggttattt accattctac cgatctggtt
     1561 cattggcaag ccattgccca gccggtgagc gccgcctggt cgaattatgg cgatcaagcc
     1621 ggtggcggtt gctggggcgg ccaaatggtt tattataacg gtgaatactg gcatttcttt
     1681 tcgcggcaga atacgatgta ttatgtcaaa gcatccgcgc cggaaggtcc ctggagtacg
     1741 ccggtcaaaa tcaataatcc atctaccctc ccgtatggtt tgggatacga taattccatt
     1801 tttattgatg acgaccataa atggtattta gtagtgaaaa atggacaacc caataacggc
     1861 atcgttgaac tcggcaatga cggacaaccg actggcgtcg tctataatct caattggtta
     1921 aatccaggtc cggactatcc ttacagttgg gcggaaggtc cggtgatgtg gaaatataac
     1981 ggctactatt actattcatt tgcccgtgac cttggcggag gtcaaaaagt tatgcgcagt
     2041 cgcactctga ctgccgatca gtccgcctgg gaaatgttgg gcgatttttt caatgaaaac
     2101 gatccccaaa agggctcctc acttttcacc agtcccaatc atgcttcagc agctgttatg
     2161 ctcgatgaca gtacttcgtg ggttatccat ccactctatg ccaagggaga atggaaaggt
     2221 cagggacggc agggattgtt aaatcaagtt cattatgatg cgaacggtaa accagtggct
     2281 gattatccgg ttaataagcc gtttactgcg ccgaaattgc cgagtagcgg tattccctgg
     2341 atggtgcctc actcggattt ctttacctcg tctgagttgc atccggaatg gtcatttttg
     2401 ggttatacac cagccgttaa atattctctc accgatcgcc ccggttggtt gcgtttatcg
     2461 cccaaaaata aagcgctagc taacacggta atcaaaaacg acggcgaaca taattattca
     2521 cttattacac gactggattt taatgctcaa tcaactaccg atcaagccgg tctctggatt
     2581 atgagaggtg atcaaacgat ggaagtcaaa cttgttagca ccttatcgac tgaaggactc
     2641 aaggtgataa cctatagctt caaatctact atatacgaga ctgaaaacac catcggcgat
     2701 actctctggt taaaattggt tcgtataaat cataaaatca gcggatattt tagcggcgat
     2761 ggattcgatt ggatcgaaat aggctcccct atagatattt cggaaataga ttcctactcc
     2821 gatttctcga cctttaccgg tacgcgccaa ggcctgtacg tgaaagggat gagcgatgcc
     2881 tggttcgatt tttatattta tcgcgatgct tatacgccga taatggctga atgcccagcg
     2941 aatcagttcg gcactgcgtt agtatggaat aaatacccgc caaattcact gggcaaaatc
     3001 cacaacgatg attgggcatt gtacgccggg gttgagttcg gcaatactga atatgcgaaa
     3061 aattgtgatt cactaaaaat aaccgcttca tgccttattg ccggaagcgc ggtggaagtt
     3121 taccttgatt cgatagatgt aaatactaaa attgcggttt gcgccattga tactaccggc
     3181 tcatggacga attttcggac ttttaccaca gatttattga aaccgatatc tggaaatcat
     3241 gacgtatatt taaaattcgt tggtcctgga agtggggaat tatttcgact ccaatcgttc
     3301 caatttatta ccaattccaa acccaccgct atcaatagtc gcccggaaaa taatctaccg
     3361 cgaagctttt atcttggaca gaattaccct aatcccttca atccggtaac tcgaataaag
     3421 tattcggtac cgaaagccgg ttacctgacc atcaaggttt atgatgtgat ggggcgagag
     3481 gt
//
LOCUS       DAOSMP010000113         3453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_27397_length_3453_cov_13.898764, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000113 DAOSMP010000000
VERSION     DAOSMP010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27397_length_3453_cov_13.898764"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PKX09_10020"
     CDS             complement(<1..421)
                     /locus_tag="PKX09_10020"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOU18197.1"
                     /translation="MSYLLPEPLKTGGTIGVIAPSSPAHRKTTAAGLQYLKDRGYKVK
                     TAPNLNRGKFFLAGSDIERLKHLEQFLCDPEIEALFCVRGGYGLIRIIDQLDYQRLVK
                     IKPKVLVGYSDITILQMALAAKLGWVTYSGPMVSSDLG"
     gene            606..2222
                     /locus_tag="PKX09_10025"
     CDS             606..2222
                     /locus_tag="PKX09_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU18198.1"
                     /translation="MKLSPKTIVNSLIITCFLFLNEAGFTQDKEMIDKVSGRMEYDFQ
                     QMTEENALGDVNTLRQFIADSLKFIYDERYQQELDSLESNYITTIKALETECTRLNNL
                     NKALTDSLEKSVASKPKSVILTKELDPALEARYFQYGKILRLEESKRKAGFLKMSAVI
                     ENIAPFQIEELNRYYDQYFPIARSDSALDFIIQINIREKDWSNAGRNIIKFLYLFPNS
                     ALYDEIKTVRAGIFQTEKYYQSYRTFLTDLLNKIPQLPLADQRYYQFVESLKDFPDPT
                     IRTTFIPEVHKFLELYPNSRYATQANFWIAEALLANNQPHSAYLTLQKIMILYPNNEL
                     YCRALHTSGVIQQEQFGEYKNAIQTFTDLIKRFPQDTLAENAHYRIAKISDENLNDWE
                     LAVEQYQIYADKYPQSPNAIPALMRKAVIQSTQMNLIEEAVRTYKSIDERYPSTIGAF
                     NALTAAGNLYTTKERYDQAIEIYMSIFQKYPQSENAVAALEKVYDIYQTKIKDNEKSI
                     EILNLIINNYPDTKASARATKQLKKFEKAQ"
     gene            2252..>3453
                     /locus_tag="PKX09_10030"
     CDS             2252..>3453
                     /locus_tag="PKX09_10030"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOU18199.1"
                     /translation="MANRLTRNINRYRFIFNGFVSVITLSLIVTSLAAQTITRPKIGL
                     ALSGGGAMGFGHIGTLKLLDSLNIPVDYIAGTSMGGIIAAFYAVGYSGAEIEEIARKV
                     DWADLFNDAPSRQALPYFQKQDADKYQFELGIRDFRPVDKGGVIAGQKITLYMTRLVL
                     PFLTVSDFDSLIIPFRCIAVDLTTGSEVVIDHGSLPIAMRATMAIPSIFTPVVWGDSL
                     LVDGGLLNNLPVNTARKMGADIVIASTVRSPFKTKEELRTTLDILSQTSNILRDNKLE
                     AEAKNADLLINCQLEKLGAADFSTAKVGKIIDAGEAAAHAKLDELLELKKKYNLSRYG
                     PENINDLNLPSGYRVARLTIKGCQTIPVDVVRSTIGIKVGELYSPEILTAGVERLQSF
                     DNYVRVRP"
BASE COUNT         1029 a          729 c          718 g          977 t
ORIGIN      
        1 gtcccaaatc agaagaaacc attggcccgc tatacgtcac ccaaccgagt ttagcggcca
       61 gagccatttg caaaatggta atatcggaat agccaaccag tactttcggt tttattttca
      121 ctaaacgctg ataatcgagc tgatcaatta tccgaattaa tccgtagcca ccgcgcacac
      181 aaaagagggc ctctatttcc ggatcacaaa gaaattgctc gagatgtttc aggcgctcga
      241 tatccgaacc agccaggaaa aatttaccac gatttagatt cggtgcagtc ttaactttat
      301 aaccacggtc tttgagatac tgtaaaccag ccgcagtagt tttacgatgc gccgggctgg
      361 agggtgcgat gacgccgatt gttcctccgg tttttagcgg ttcaggcagt aaatatgaca
      421 caattctctt ccactatttc ttcataaagt tgcatattta aaacgaaaag cgaaagcatt
      481 tttaactttt ggttggtaaa agtgaaaata ttagtattgt gaattttgca accattagat
      541 ttttcagcca gattttccta attttgtgcg agtaatgttg agatttatgt taatatgagg
      601 ctaaaatgaa gttatcacca aaaactattg tcaattcatt aattatcact tgttttttat
      661 ttctaaatga agccggattt acccaggaca aagaaatgat tgataaagtc agcggtcgga
      721 tggaatatga ttttcaacaa atgactgaag aaaatgcgct gggggatgtt aacactctcc
      781 ggcaatttat tgccgattct ctcaagttta tctatgatga aagataccag caggaacttg
      841 attctctgga atcaaactac attactacga ttaaagccct ggagaccgaa tgtactcgcc
      901 tgaataattt gaataaagct ttaaccgata gtcttgagaa atccgtggct tcaaaaccga
      961 aatctgtaat tttaaccaaa gaactcgatc ccgctcttga agccaggtac tttcaatatg
     1021 gaaaaattct caggctggag gaatccaagc gtaaagccgg ttttttgaaa atgagcgccg
     1081 tgatcgagaa tatcgcgcct ttccaaattg aagaattgaa cagatattat gatcaatatt
     1141 ttccaatcgc gcggagtgac tcagctctcg attttatcat ccagattaat atacgcgaaa
     1201 aagattggag caatgccggt cgcaatatca tcaagttcct ttatcttttc cccaattcag
     1261 cgctctatga tgagattaaa accgttagag ctggtatttt ccagacggag aaatattacc
     1321 aatcttatag aacatttcta accgacctgc tgaataagat tcctcaatta ccgctcgccg
     1381 accaacgtta ttatcaattt gttgaatcgc tcaaggattt ccctgatccg acaattcgaa
     1441 ccacctttat accagaagtc cataaatttc tggaactata tccgaattcc agatatgcca
     1501 cgcaagccaa tttttggata gcagaagcac ttttagctaa caaccagcca cacagcgcct
     1561 atctgacatt acagaaaata atgatactct atcctaataa tgaactatac tgtcgcgctc
     1621 tacataccag cggggttatt caacaggaac agttcggtga atataaaaat gcgattcaga
     1681 ctttcactga tttgattaaa cgctttccgc aagacaccct ggctgaaaat gcccattatc
     1741 gcatcgctaa gattagcgac gagaatttaa atgattggga actagcggtc gaacagtatc
     1801 agatttatgc cgataaatat ccccaatcgc cgaacgccat tccggcttta atgcggaaag
     1861 ccgtgattca atctacccag atgaacttaa ttgaagaagc cgtcagaact tataagtcca
     1921 ttgatgaacg ctatccttct acgattgggg catttaacgc cctgacggct gcgggtaatc
     1981 tttatacgac caaggagcgc tatgatcagg ctattgaaat ttatatgagc atttttcaga
     2041 aatatcccca atcggaaaat gccgtcgcag ctctcgaaaa agtctatgac atttatcaaa
     2101 cgaaaatcaa agacaacgag aaaagcatcg aaattttaaa tcttatcata aacaactatc
     2161 cggataccaa ggcctcagct agagcaacta agcagttgaa gaagttcgaa aaagcccaat
     2221 agtctgcaat aaataaacta tgaactttcc aatggcgaat cgccttaccc gaaatattaa
     2281 tagataccga ttcattttca atggatttgt ttctgttatt actctatctt tgattgttac
     2341 cagtttagcg gctcaaacaa ttactcgtcc taaaatcgga ttggctctaa gtggcggtgg
     2401 ggcgatgggc tttgggcata tcggcaccct gaaattgctg gattcgctga acattccggt
     2461 tgattatatc gctggcacca gtatgggcgg aattatcgct gccttttacg ctgtcggtta
     2521 ttccggcgcg gaaatcgaag aaattgctcg taaggttgat tgggcagatt tgttcaacga
     2581 tgcgccgtcc cgacaggctt tgccctattt ccagaaacag gatgccgaca aatatcaatt
     2641 tgaattaggt attcgtgatt ttcggccggt agataaaggg ggcgtgatcg ccggccaaaa
     2701 aattacgctc tatatgactc gcctggttct gccatttttg actgtcagtg attttgacag
     2761 tctgataatt ccatttcggt gcattgcagt tgatttgacc actggatcgg aagtggttat
     2821 agatcatggt tctctgccga ttgcaatgcg agcaacgatg gcaattccca gcatttttac
     2881 tccagttgtt tggggcgact cacttctggt tgacggcgga ttgctgaata atctgccggt
     2941 caacactgcc cgtaaaatgg gtgctgacat cgtaatcgct tcgacggtta gaagtccttt
     3001 taaaactaag gaagagctac gaacgaccct ggacattctg tctcaaacat ccaatatcct
     3061 gcgcgataat aaacttgaag ctgaagccaa aaacgccgat ttgctgatta actgtcagct
     3121 ggaaaagtta ggtgcggctg atttctcgac agctaaggta ggcaaaatta ttgatgccgg
     3181 cgaagccgcg gcgcacgcca agctcgatga attattggaa ttgaagaaga aatataacct
     3241 atcaagatat ggccctgaga atattaatga tttgaattta ccaagtggtt accgagtcgc
     3301 tcgccttacg ataaagggtt gtcaaaccat tccggtggat gtcgtccgtt cgactattgg
     3361 tattaaagta ggcgaattgt attctccgga aattcttacg gcgggtgttg agcgtttgca
     3421 atcttttgat aattatgtcc gggtaagacc gat
//
LOCUS       DAOSMP010000114         3441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_27549_length_3441_cov_3.438275, whole genome shotgun sequence.
ACCESSION   DAOSMP010000114 DAOSMP010000000
VERSION     DAOSMP010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27549_length_3441_cov_3.438275"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PKX09_10035"
     CDS             complement(<1..1101)
                     /locus_tag="PKX09_10035"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOU18200.1"
                     /translation="MKKIIRIIIPAFLLATCLVSLLWSGTTGKITGIITDKKTGEPII
                     GANVIVMGTSLGAATDQNGQYTILYVPPGVYNVNISVIGYAKTTLSDVEVHIDQTTRL
                     DVNLEAEAVVGETVVVVAERKIIKRDVATSSVAVSANEIESLPASNVDDVISMQAGIR
                     GNMAIRGSESEDILFLVDGITVRDPRNNEPVTKIPLSAVKEISVERGGFNAEYGQVQA
                     GLVNVVTREGDKSNYSGNFQIKYSPPAPKYFRVDGIPDVHDPDSYWMRPYLDDAVCWT
                     GTTQGEPFTDLNGNGKWDTGEDFEDLNSDGSHTYWDQYMRDQYPSFVGWNEISRQLLT
                     DNDPTNDLTPLGAQRVFLYETRKKQVNDMPDYD"
     gene            complement(1447..>2060)
                     /locus_tag="PKX09_10040"
     CDS             complement(1447..>2060)
                     /locus_tag="PKX09_10040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU18201.1"
                     /translation="PVFTCGSGWPGNTGDDVSATPITAGPVWIVAMVKMSGGTGSDRT
                     YMWIDPDPSVEPDTNDAIVKRNSSMPEGFDHIALEFGGDGADVRLVFDEITIASSFAD
                     LTKTTGISRKETVSFEYSLSQNYPNPFNPTTNITYSLKQTGEVRLSVFDILGREVAVL
                     VDDVQNAGQHTITFSGTDLSTGIYLYRLQIGKEVITKKMVLVK"
     assembly_gap    2061..2160
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2161..2687)
                     /locus_tag="PKX09_10045"
     CDS             complement(<2161..2687)
                     /locus_tag="PKX09_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18202.1"
                     /translation="MRMRTVVISVVLIMALSSIAMPQSLVRESFSYPATTLAGLGTAS
                     NGFSGSWVTDEADGIEGLVAVSGTRFAYADLNYEIPHDTLHLQVVKSNAWADHNRYKR
                     PLDGAWPNEAGKKYWVSYLLDIKEPLPVGDTYFMVKLYKGAGELVAIGKGGGGPTPPV
                     FTCGSGWPGNTGDDVS"
     gene            complement(3054..>3441)
                     /locus_tag="PKX09_10050"
     CDS             complement(3054..>3441)
                     /locus_tag="PKX09_10050"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOU18203.1"
                     /translation="RERFSHMVYMNPKEINVSSPDEQFLQRTVEAVEQYLSDSGFSVD
                     DLSYHVGLSRAQLYRKMQALINQTPSEFIRNYRLQRAMQLLEKGHSPSQVCYQIGFKD
                     PSYFTKCFRKQFGRTPQQIRPESELS"
BASE COUNT          978 a          810 c          601 g          952 t
ORIGIN      
        1 atcgtaatca ggcatatcat ttacttgctt tttacgggtt tcatacaaaa acacccgttg
       61 agctcccaga ggtgttagat catttgtggg gtcattatca gtcaatagct ggcgagaaat
      121 ttcgttccag ccgacaaacg acggatactg atcacgcata tattgatccc agtaggtatg
      181 acttccatca ctgtttaaat cttcaaaatc ttctcctgta tcccatttcc cattgccgtt
      241 taaatcggta aaaggttcac cctgagtagt tcccgtccag cagacagcat catcgagata
      301 gggacgcatc caataggaat cgggatcgtg gacgtccggt atgccgtcaa cccgaaaata
      361 tttaggcgcc ggcggagaat actttatctg aaaattacca ctataattgc ttttgtcccc
      421 ttctctagta acaacattga ccaatccggc ttgaacctgc ccatactcgg cattaaaacc
      481 tccccgctcg accgaaatct ccttcaccgc actcaatgga attttggtta ctggttcatt
      541 attacgcgga tcgcggaccg taattccgtc taccagaaac agaatatcct cagattcact
      601 accacgaatt gccatattgc ctctaatacc cgcttgcata ctaatgacat cgtccacgtt
      661 tgatgctggt aaactttcaa tttcattagc tgaaacggct acactactgg tagccacatc
      721 tcttttgatt attttacgtt cagccacgac tactaccgtc tcaccgacaa ccgcttctgc
      781 ctccaagttc acatccagcc tagtagtctg gtcaatatgt acctcgacgt cactaagcgt
      841 cgttttcgca tatccgatta ccgaaatatt gacgttataa acacccggtg gcacatacaa
      901 tatcgtgtat tgcccgtttt ggtcagtagc cgctccgaga gaagtcccca tcacgatgac
      961 attggctcca atgatgggtt ctccggtctt tttatcggta ataataccgg taattttccc
     1021 ggtcgttcct gaccaaagca agctcaccag acaagtggct aacaaaaagg caggaattat
     1081 tatcctaata atttttttca ttctcaattc ctcctttcag agagaaaacg gaaaaatcct
     1141 gatatggatt tagcaaaatt tgaatataaa tcacacattt tattctttta aaaatcccca
     1201 taaatatttt taacctcgcg atattttcgg tcgcttcaga ttaattattt cttatgctcg
     1261 attaatattc atctcgttaa ttgaatcgag taaatagccg tattgtaaaa gaacaacttc
     1321 cttgcattta attgtaacag gataagggaa ggcgctttgc aacgccttcc cctattgtca
     1381 cccctcattg atcaaaaatg tgaaaaaact aaacaaattt cacattcgcc gaattgaaaa
     1441 aaaccgctat tttaccaaaa ccattttctt agtgattact tctttgccaa tctgcagtct
     1501 atacaggtaa atgcccgtgg ataagtcagt accggaaaaa gtaatcgtgt gttgcccggc
     1561 attctggaca tcatcaacca gcaccgctac ttcccttccc aaaatatcaa atactgacag
     1621 gcggacctcg ccagtctgtt tgagagaata agtaatattg gtcgtcggat tgaacggatt
     1681 cggatagttc tgagacaaag aatattcaaa tgatactgtc tctttacgac ttattccagt
     1741 tgtcttagtt aggtcggcaa aagaggaagc aattgtgatt tcatcaaaaa ctaacctgac
     1801 atcagctcca tcgccaccaa attccaatgc aatatgatca aatccttccg gcatcgaaga
     1861 attacgtttc actatggcat cattcgtatc cggttctacg ctcggatccg ggtctatcca
     1921 catataagtc cgatcagaac cagtgccacc tgacatcttc accatcgcca ctatccatac
     1981 cggcccagcc gtaattggag tcgcagatac atcgtcaccc gtattacccg gccaaccact
     2041 accgcaggtg aacacaggag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 gatacatcgt cacccgtatt acccggccaa ccactaccgc aggtgaacac aggaggtgtc
     2221 ggtccgccgc cgcctttacc aatcgctacc aactcaccag cacctttata caacttgacc
     2281 ataaaatagg tgtcgccaac cggtagaggc tccttaatgt ccaacagata actgacccaa
     2341 tatttcttgc cggcttcgtt cggccaagct ccatccaatg gcctcttgta acgattatga
     2401 tcagcccagg cattactctt aactacctgc agatgaagcg tatcatgtgg aatctcatag
     2461 ttaagatcag cataagcaaa tctcgtccca ctaaccgcaa ctaaaccttc aatgccatca
     2521 gcctcgtcag tcacccatga tccactaaat ccattactcg cagttcccag ccccgccaga
     2581 gtagtcgctg gataactaaa ggattcacgt actagtgact gaggcattgc aattgagctt
     2641 agcgccataa tcaaaacgac actaataaca acagttctca ttcgcataat ttctctcctt
     2701 tgtttgtttt tttaatgcaa ttaaacgtta gaaacaacat tcttagtgat ttgtagaacc
     2761 cacacctcct ttcgtctttt ttaattatca tgaaaaaacc tcactcttag attgagtgtt
     2821 tatacaatat cacaaaaaaa acgatgatga atatgataca aataacctca atcaaaatcc
     2881 atttccaacg attacgattt tcggcgcttt caactgggtc tggtttgttt aatttctgct
     2941 tcatttgaaa ctaaagtaaa gtgtttaaaa agaaaaatca tctcaaaatc tggtaattag
     3001 gttgcatacc gattttaaag gtgaaatttg attttaaata aagaatggaa gggctaagaa
     3061 agctcggatt cgggacggat ttgctggggt gttcgtccaa attgcttgcg aaagcattta
     3121 gtgaaatagg aagggtcctt gaaaccaatt tggtaacaaa cttgagatgg cgaatgccct
     3181 ttttccagta attgcatagc tctttgcaag cggtaatttc ggataaattc gctgggagtc
     3241 tggttaatca aagcttgcat tttacgataa agctgggcgc gactcaagcc aacgtgatag
     3301 ctgagatcat caacagaaaa accgctatcc gataaatatt gttcaacggc ttcaaccgtt
     3361 ctctgcagaa attgttcatc aggtgaactg acgttaattt ctttcgggtt catataaacc
     3421 atatgactaa agcgttctcg g
//
LOCUS       DAOSMP010000115         3401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_27953_length_3401_cov_13.263598, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000115 DAOSMP010000000
VERSION     DAOSMP010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27953_length_3401_cov_13.263598"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..1317
                     /locus_tag="PKX09_10055"
     CDS             388..1317
                     /locus_tag="PKX09_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18204.1"
                     /translation="MRQVVDFGFAWNWEYDWDFANLLEADCRSEGLNFLQVTPEKIES
                     VLTEIGQGGIYLQALWDRAADTDSRFIELINWAKNNNIYQINPHEKAVRAWNKATMHL
                     EFITAGVNTPYTIILEPYNVRPVLPEIDLSVLGGRFIIKPAHGGGGLGVILDATSLER
                     VLTARRENPDDFYLLQAVIEPLQVNGRLAWFRVIYGFGKAYFCWWDVKTHIYTPFSNN
                     DKESLPMPLLNEIIEKIADVTGLDVFSSEIALTAEGKFVVVDYINDPVDLRLQSRAAD
                     GVPDEIVRAISTDCVATLKKAVRVNVTYEKLEN"
     gene            1544..2092
                     /gene="pyrR"
                     /locus_tag="PKX09_10060"
     CDS             1544..2092
                     /gene="pyrR"
                     /locus_tag="PKX09_10060"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOU18205.1"
                     /translation="MEFRVKARLADEQGVNRTITRLAHEIIENCKGTENVGIIGIRTR
                     GDYLAKRLAHKIEEIENVKLPVGVLDVVMYRDDFRMKTKLPRVEVTDIPFNVDGKILI
                     LVDDVIYTGRTIRAALDALMDFGRPAAIQLAVLVDRGHRELPIKPDYVGKNVPTSIGE
                     EVRVMIKEIDGKDEILLVEEQK"
     gene            2089..3009
                     /locus_tag="PKX09_10065"
     CDS             2089..3009
                     /locus_tag="PKX09_10065"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOU18206.1"
                     /translation="MNLSIRHLLGLEGVPRDDISTILDTAFSFREVLERPIKRVPTLQ
                     GKTIVNLFFENSTRTRISFELAEKRLSADTVNFSSSGSSLAKGESLKDTVRNLYAMKT
                     DAVVMRHGVPGSAKLLTQFIDAVVINAGDGTHEHPTQALLDMMSLQEKFGSIDGLNVA
                     IIGDIRHSRVALSNIYGLLTMGANVALCGPPTFMPVGIESLGVKVIYDLDEALAFADA
                     INILRIQRERQGKGMIPSLREYRAVFGITRERLDKLHKTITLLHPGPINRGVEIDSDV
                     ADSEHSLILHQVLNGVAVRMAVLFLLLGGK"
     gene            3032..>3401
                     /locus_tag="PKX09_10070"
     CDS             3032..>3401
                     /locus_tag="PKX09_10070"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU18207.1"
                     /translation="MQIKAINEELLLQNAVIVDPFKDNIYEADILIEKGKIKNIEQKI
                     DPKNVKICEDLKGSYVAPGFIDLHTHLREPGNEDEETIATGVRAALAGGFTKICCMPN
                     TDPPIDTQEAVRFIYRQAENL"
BASE COUNT          997 a          674 c          793 g          937 t
ORIGIN      
        1 tccgacattt cactctggtt ctcgactcat tgtcgttaaa acacttactt ttttcggtaa
       61 aaaaatcccg ctcaaataat cttattttca gtaatgagca tattattaat ttttttataa
      121 aaatcacgag ggatgaaacc gttcagaaac caatagtttg gaaaattggg ttcataaagc
      181 tgcaaaagtt agttttctat tcctcggttc attatcggat aggtaaaatt tcatcgagta
      241 ataacttttg atagctcgtt taaaaatctt aacttttgtt aaatgattag tgataataat
      301 ggttttattt aaaatattat cttttgtgca atttgatgaa ttatcctgat attgacctgc
      361 gtttaataac cgaaaagtgg attttgtgtg cggcaggtag ttgatttcgg ctttgcctgg
      421 aactgggaat atgattggga ttttgccaat ttattagaag ctgattgccg gtcggaagga
      481 ttaaattttc ttcaagtgac tccagaaaag atcgaatcgg tactgactga aatagggcaa
      541 ggtggaattt atttacaagc attatgggat agggcggctg acaccgatag ccgatttata
      601 gaactgataa actgggctaa gaataacaat atttaccaga tcaatcctca cgaaaaagcc
      661 gtcagagcct ggaacaaagc gacaatgcac ctggaattta tcacagcagg agtcaataca
      721 ccttatacga ttattttgga accttataat gtccggccag tattgcccga gattgacttg
      781 agtgtcctag gcggtcgttt tataatcaaa ccggcgcacg gcggcggcgg cttgggtgta
      841 attctggacg cgaccagtct ggagcgcgta ttgactgctc gtcgagaaaa tccggatgat
      901 ttttatcttt tacaggctgt aatcgaaccg ttacaggtca acgggcggct ggcttggttt
      961 cgggtcattt atggcttcgg aaaggcttat ttttgctggt gggatgtcaa gacgcatatt
     1021 tatacccctt tttcgaataa tgataaagag agcctaccca tgccattact aaacgaaata
     1081 atcgagaaaa ttgccgatgt gacgggactg gatgttttct cctccgaaat tgctctgacg
     1141 gcagaaggta aatttgtggt ggtagattat attaacgatc cagttgattt gcgtttgcaa
     1201 tccagagcgg cagatggtgt cccggacgaa atcgtccgag cgatttcaac ggattgcgtt
     1261 gccactctaa aaaaagctgt tcgggtaaat gtaacttatg aaaaattgga aaattagaga
     1321 attagataag ttgatattta attcactcca gagaaatcta actgtttatc tatgggttat
     1381 agatttttcg attacgaaaa atactttggc tttattatga ctatcatcta aattaatgta
     1441 attaaaaaag gagcctttaa aaccaaaccg tgagttggga aaggcaaaag agaaaccaga
     1501 aatccaccca gagcggtggt tttttatttc aggagtatca actatggaat ttagagttaa
     1561 agcccgtctg gcggacgagc agggagtaaa tcgcacgatc acgcgattgg ctcatgaaat
     1621 tatcgagaat tgcaaaggaa ccgaaaatgt cggaatcatt ggtattcgca cgcgtggtga
     1681 ttatttagcc aaacgtttag cccataaaat cgaagagatc gaaaatgtca aactaccagt
     1741 cggtgtcctt gatgtcgtta tgtatcggga tgattttcga atgaagacca aattaccgcg
     1801 cgtcgaggtc accgatattc ctttcaatgt ggacggcaaa atcctaatcc tggtagatga
     1861 tgtaatttat actggcagaa cgattcgcgc cgcactcgat gccctgatgg atttcgggcg
     1921 accggcggcg atccaattgg cagtgctggt ggaccgtggt caccgcgaac taccgatcaa
     1981 acccgattat gtagggaaaa acgtaccaac ctcaattggt gaagaagttc gcgtaatgat
     2041 taaagaaatc gacggtaagg atgaaatcct gttagtggag gaacagaaat gaatttgagt
     2101 attaggcatt tactcggact ggaaggtgtg ccgcgggatg acatcagcac tattctggat
     2161 acggcttttt cattcaggga agttctggaa cgtccaatta agcgtgttcc gactcttcag
     2221 ggtaagacca ttgtcaatct tttcttcgag aattccacca ggacgcgcat atcatttgaa
     2281 ctggccgaga aacgtctttc agccgatacg gtcaattttt catccagcgg aagtagcctc
     2341 gccaagggcg aaagccttaa agataccgtc cgtaacctct atgctatgaa aacagatgcc
     2401 gtcgtaatgc gacacggcgt gcccggctca gccaaactac tgacgcaatt catagatgcc
     2461 gttgtaatca atgccggtga cggaactcat gaacatccga cccaagccct tttggatatg
     2521 atgtcgttgc aggaaaaatt cggttcgatc gacggcttga atgtcgcaat cattggtgat
     2581 attcgccaca gccgagtagc gctctctaat atttacggat tgttaactat gggcgccaat
     2641 gtcgcgctct gcggaccacc gacgtttatg ccggttggga tcgaatcgct gggcgtaaaa
     2701 gtaatctatg atctggatga agctttagcc tttgccgatg ctatcaacat cctgcgcatt
     2761 cagcgggaac gacaaggtaa aggtatgatc ccatcactac gcgaatatcg agcggttttt
     2821 ggcatcacgc gcgaacgcct ggataaattg cataaaacga ttacgctact gcatcccggc
     2881 ccgattaatc gcggagtgga aattgactct gacgttgccg atagcgagca ctcactcatt
     2941 cttcatcagg tgctgaacgg cgtcgccgtg cgtatggcag tactttttct attattgggt
     3001 ggcaaataaa tggaattatt taggagtaaa aatgcaaatt aaagccatta acgaagaatt
     3061 acttttacag aatgcggtga tcgttgatcc attcaaagac aacatttatg aggcagacat
     3121 tctgattgaa aaaggtaaaa taaaaaatat tgagcaaaag atcgacccga aaaatgtaaa
     3181 aatatgtgaa gacctcaagg gatcatacgt tgcgccggga tttattgacc tgcatactca
     3241 tctacgcgag cccggcaatg aagatgagga gacaattgca accggagtca gagccgcgct
     3301 ggctggcgga ttcactaaaa tctgttgtat gccgaacacc gatccaccca ttgatactca
     3361 agaagccgtg cgttttatct accggcaagc cgaaaacctt t
//
LOCUS       DAOSMP010000116         3318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_28831_length_3318_cov_12.025437, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000116 DAOSMP010000000
VERSION     DAOSMP010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28831_length_3318_cov_12.025437"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1585)
                     /locus_tag="PKX09_10075"
     CDS             complement(<1..1585)
                     /locus_tag="PKX09_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOU18208.1"
                     /translation="MPNNLILMVTLPIVIAVLNTFLPAILKKVLTFLGLGYLLYLCYD
                     LFTGAAEAVYLFGQPILGMDKMALFALIFIQVMSFIILVFSLKGVDKGIERGFFILYP
                     MTVGISNGIVLSVNTFGLLIFWGLSGLMLYLFGLLGQTSSAPQTAKKTFIIVGGSDVF
                     LILGLILIWFLGAGNSWSLWNTQIVMSGGLAYVAFFSILIAAFAKAGGFPFHTWLPDF
                     SQDAPLESVAFLPASLDKLVGIYLLARLSVSLFVVNLNIQMILISLGALTVICAVMMA
                     LIQHNGRRLLGYHAVSQVGYMIIGVGSGSVIAFIGGLFHMVNHTIYKSTLFLTLNSIE
                     KQTGTANLDELGGLGRNMPLTFIAALTGALAISGIPPLNGFYSKWLIYQGLIEKMQSL
                     TTGYQIWLLICLILAVFGSALTLASFMKFIHAIFLGKRPKKWKDIKEAPFNQWLSVGV
                     LALLCVVFGLFAKPLPIGKFIAPIISELGLPMPVVLGSYEPVQLFLLFGTAFLLGLVI
                     YLLVKNVRFDENYLGGMPSL"
     gene            complement(1575..2762)
                     /locus_tag="PKX09_10080"
     CDS             complement(1575..2762)
                     /locus_tag="PKX09_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOU18209.1"
                     /translation="MIENKIPLGPFHPLLEEAEYFELKVDGETVVDIDMEIGWMHRGH
                     EFISESKTFTQSIYLVERICGICSTSHPLACVHAIEDIDNIEIPLRARYIRTLIGELE
                     RIHSHLLWLGLAGHFIGYDTLWMWAWKYREPILEMFEAITGNRNHYGMMRVGGVARDV
                     DSTIIPKLLKILDNVDRKMDMLAKAAHDDPVLKSRLKGVGVLTKADAIAYCAVGPVAR
                     ASGVAIDVRKDEPSDAYDLVEFDEIVRSNGDVYDKLVLRILEILESSKIVRQCLEKLK
                     VVEGPILNNVKEIMPGEGIGRYEAPRGEVFHYIRSDGSNRPVRHKVRAPSYVNIPTYT
                     ASCKGIPVADALITLAAVDPCYCCTERALRVIDPLNRPCEIDLLKLSRAKTEQIRRES
                     YAE"
     gene            complement(2759..3190)
                     /locus_tag="PKX09_10085"
     CDS             complement(2759..3190)
                     /locus_tag="PKX09_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOU18210.1"
                     /translation="MDEYKRFLELMGDKVTQVIEKPRRLYFVVSNENLKEIARYLFVT
                     LGCRLSTATAQETYHGLEILYHFSHDASGCYYCPRVVLTDKKNPKVNTITDIVTGALW
                     IEREIFDLWGIEFVGHPRMEQLLTLNSPENPDKPLRFGRSL"
     gene            complement(3183..3296)
                     /locus_tag="PKX09_10090"
     CDS             complement(3183..3296)
                     /locus_tag="PKX09_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18211.1"
                     /translation="MPSREAVIPIDMYIPGCPPKPEAMIAAVAKLIGVLNG"
BASE COUNT         1010 a          873 c          652 g          783 t
ORIGIN      
        1 ccagagacgg catcccgcca agataatttt cgtcaaaccg aacgtttttg accagtagat
       61 aaataaccaa acccaacaga aatgccgtac caaacagcag aaacaactgc accggttcat
      121 atgagccaag aactaccggc atcggtaaac caagttcgga tattatcggc gcaataaatt
      181 tcccgatcgg taaaggctta gcgaataatc cgaaaacaac acataacaag gctaatacac
      241 cgacggataa ccattgattg aaaggcgcct ctttaatatc cttccatttc ttgggacgtt
      301 tcccgagaaa aatagcatga atgaatttca taaagctggc taaggtcaaa gcacttccga
      361 aaaccgccag tatcaggcaa atcaagagcc aaatttgata accggttgtc aaactctgca
      421 ttttttcgat taatccctgg tagatcagcc atttcgagta aaaaccgttt agcggcggaa
      481 ttcccgaaat cgccagcgcc ccggtcaaag ccgcgatgaa cgttagcggc atattgcgcc
      541 ccaaacctcc caattcatcc agattagcgg ttccggtctg cttctctatc gaatttagcg
      601 tcaggaaaag tgttgatttg taaatcgtat ggttaaccat atgaaataaa ccgccgataa
      661 aagcaatcac actcccactc ccaactccaa taatcatata accgacctga ctgacggcat
      721 ggtaaccgag taagcgccga ccattatgtt gaatcaacgc catcattacg gcacaaatta
      781 ctgtgagagc gcccagcgat attaaaatca tctgaatgtt gagattaact acaaataatg
      841 aaactgacag tcgcgcaagc aggtaaattc caaccaattt atccagcgaa gccggtaaaa
      901 aagccacact ttccagcggt gcatcctggc taaaatccgg caaccaggta tgaaacggaa
      961 atccaccggc tttggcaaaa gccgcgatca gaatactaaa gaaggcgacg taagccagac
     1021 ctccgctcat aactatctgc gtattccata aactccaaga attacccgct cccagaaacc
     1081 agattagaat caatccgaga atcaggaaaa catcacttcc gccgactata ataaaagttt
     1141 tcttggcggt ttgcggcgcg ctggaagtct gtcccaatag accaaacaaa tacaacatca
     1201 aaccagaaag tccccagaaa atcagcaagc caaaagtatt gacactcaga acaattccat
     1261 tactaatgcc aactgtcatc ggatacagaa taaaaaatcc gcgttcgatg cccttatcaa
     1321 cgcccttcag tgaaaaaacc aaaataataa aactcatcac ttgaataaaa atcagggcaa
     1381 aaagcgccat tttatccatt cccagaatcg gttgaccgaa aaggtaaacc gcttccgccg
     1441 ctccggtaaa taaatcgtaa cacagatata gcaaatagcc cagcccgaga aaggtcaaca
     1501 ctttcttcaa aatcgctggg agaaatgtat tcaacaccgc gataacgatc ggcaatgtca
     1561 ccatcaaaat caaattattc ggcataactt tccctccgga tttgttcggt tttggcgcgt
     1621 gaaagcttca gcaagtcaat ctcacaaggc cggttcaagg gatcaatcac tctcagcgcc
     1681 cgctccgtac agcagtaaca gggatccacg gcggctaaag taattaacgc atcggcaacc
     1741 ggaatcccct tacacgaagc cgtataagtc ggaatattca cataactggg ggcgcgcact
     1801 ttgtgtctga ccggtcggtt actgccatct gatcgaatat agtgaaaaac ttcaccgcgc
     1861 ggtgcttcgt agcgtccaat tccttcgcca ggcattattt ccttgacatt attcagaatc
     1921 ggtccttcta ctacctttaa tttttccaga cattgacgaa caattttact agattccaga
     1981 atttccagaa tacgtagcac cagtttatcg taaacgtcac cattcgacct tacaatttcg
     2041 tcaaattcaa ccagatcata cgcatcactc ggctcgtctt tacgaacatc aatcgccacc
     2101 ccggatgcgc gcgctaccgg cccaacagca cagtaggcaa tggcatcggc tttagtaagc
     2161 acaccgacgc ctttcaaacg cgacttcaac accggatcgt catgcgcggc tttggcgagc
     2221 atatccatct tgcggtcaac attgtccaga attttcagta atttcggaat aatagtagaa
     2281 tcaacatcgc gggcaacgcc acccacgcgc atcataccgt aatgattgcg gttgccggta
     2341 atggcttcaa acatttccag aattggctcg cggtatttcc aagcccacat ccaaagcgta
     2401 tcatatccga taaaatgccc cgctaacccc aaccacaata aatggctatg aatccgttcc
     2461 agttcaccaa ttaaggtgcg gatatagcgc gctcgcagtg gaatttcgat attatcaata
     2521 tcttcgatgg catgcacgca agccagcgga tgcgaagtcg aacagatacc gcaaattctc
     2581 tcaactaaat aaattgactg cgtaaaggtc ttgctttccg agataaattc atgtccgcga
     2641 tgcatccagc cgatttccat atcaatatca accaccgtct caccgtccac tttcagttcg
     2701 aagtactcgg cttcttccaa tagcggatga aatggaccaa gtggaatctt gttttcgatc
     2761 ataacgacct cccgaagcgc aacggtttat ccggattttc cggactattc aacgtcagga
     2821 gttgttccat acgcggatga ccgacgaatt cgatgcccca caggtcgaaa atctcgcgct
     2881 cgatccacag tgcaccggtc acgatatcgg tgatagtatt aacctttgga ttcttcttgt
     2941 cggttaaaac cacccgtggg caataatagc aaccactggc gtcgtgcgaa aaatggtaga
     3001 ggatttcgag accatgatag gtttcttgag cggtggcagt tgacagccga cagccgagcg
     3061 taacgaacaa ataccgggca atttctttga ggttttcatt cgacaccaca aaatagagcc
     3121 ggcgcggttt ttcgattacc tgcgtaacct tatcgcccat taattccaaa aatcgtttat
     3181 actcatccat ttaaaactcc tatcagtttc gctaccgcgg caatcatcgc ttccggcttc
     3241 ggcggacagc ccggaatgta catgtcaatc ggtatgaccg cttctcgtga cggcacaata
     3301 ttgtagctat ctttgaaa
//
LOCUS       DAOSMP010000117         3085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_31688_length_3085_cov_19.584488, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000117 DAOSMP010000000
VERSION     DAOSMP010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31688_length_3085_cov_19.584488"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PKX09_10095"
     CDS             <1..609
                     /locus_tag="PKX09_10095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU18212.1"
                     /translation="NDPPTAFELVSPENDWAGDFSACRFRWSMSSDPDREDTLAYDFS
                     LSRDSSFAILDTMINAVADTSITLDTLVSGEYYWKVCAHSNRDTVWASGSNINPWSFT
                     IVTVSINQLKPVLTEYRLYNNYPNPFNPTTTLRYELPKDSRVTLLVFDINGREIARLV
                     NETQPAGCYSLIWNAGEIGSGIYFYRIQAGEFYQVKKMVLMK"
     gene            complement(696..2675)
                     /locus_tag="PKX09_10100"
     CDS             complement(696..2675)
                     /locus_tag="PKX09_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOU18213.1"
                     /translation="MESKDSTVTKLPENAYRPLKPGEEYIPVIPDAKIVPEVTPYSLG
                     WGLFYAVLFSMAAAYLGLKIGQVFEAAIPIAILAAGTSVLLKRKNALQENVIIQSIGS
                     SSGVIVAGAIFTIPGLYILGLDASYLQIFLASLLGGFLGILFLIPFRKYFVKDMHGEF
                     PFPEATATTEVLIAGEGGGAQAKVLVSAALVGGLYDFCVAGLGFWKEVVSTSVFRWGA
                     LLADKLKWVLKINIGAAVTGLGYIVGLRYSAIIASGSFLAWWVIVPIVSYVAGLGAQT
                     PDAIFFGYVRPIGIGAIAAAGIIGILKSSKIIGGAFKLAGKELFGGSKGGTVEETSRT
                     QKDIPMKWVTVFLVLTLISIFFFFLFGVRITLKQAIVGLLIVAVISFLFTTVAARAIA
                     IVGTNPVSGMTLMTLLLSSFILVKVGLTGDHGMLAALLIGGVVCTALSMAGGFITDLK
                     IGYWLGTTPIKQESYKFLGSLFSAASVGLVILLLNKTFGFGGDSPLQAPQASAMAAVI
                     KPLMTGEQAPWVLYLAGVFLAIILEIIRVPSLAFALGIYLPIQLNTPLLFGGLIAYLV
                     SKSTKNEALAKARSDRGTLIASGFIAGGAIMGVVSAFIIFIGQNIFGDGWTLAHGIGT
                     SQWAESAGGEILGFVMFALLLVYLYWDSLRVKDKS"
     gene            complement(2737..>3085)
                     /locus_tag="PKX09_10105"
     CDS             complement(2737..>3085)
                     /locus_tag="PKX09_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18214.1"
                     /translation="FAINLQLFYICRLIMIDIEKTDKIMSSKTDKVDFSKNLSRHEKE
                     MGIQISEMEKYKWICSKDQGRDVGKMAYFEWTQKYGHKVRDWLESLSDEEIDRLFAEL
                     SDQVKNYIEEKSH"
BASE COUNT          913 a          753 c          656 g          763 t
ORIGIN      
        1 aatgatccac caaccgcatt tgagttggtc agtccggaga acgactgggc gggagacttt
       61 tctgcttgca gattccgctg gagtatgagt agcgatccgg atcgtgagga tacgttagct
      121 tatgattttt ctttgagccg tgacagtagt tttgcgattt tggatactat gatcaatgca
      181 gtggctgata cctcgataac tcttgatact ctagtttcgg gagaatacta ttggaaagtt
      241 tgtgctcata gtaatcgcga tacggtttgg gcttctggta gcaatattaa tccatggtct
      301 tttacaatcg tgaccgtttc aattaatcag ctaaaacctg tcctcacaga atatcggcta
      361 tataacaatt atcccaaccc tttcaatccg accacaacct tgcgctatga gttgccgaaa
      421 gattcgcggg taactttatt agtttttgat atcaacggtc gtgagattgc ccgactggtg
      481 aatgaaactc aaccagccgg atgctattct ctgatctgga atgccggtga gattggctcc
      541 ggaatttatt tctatcggat tcaagccggt gaattttacc aagtgaagaa aatggtttta
      601 atgaaatagc aataatctta ataataaaca aaatagcccg actaaacatc gggctatttt
      661 ttttactcgt tgactaaaaa attagtttgt aatgctcaag atttgtcttt gactcgcaag
      721 gaatcccaat agagataaac taacagcagg gcgaacatca cgaatccgag aatctcgccc
      781 ccagccgact ccgcccattg ggaagtaccg ataccgtgcg ccagcgtcca gccgtcgccg
      841 aaaatatttt gcccaataaa aatgataaag gctgaaacaa ctcccataat agcgccacca
      901 gcaatgaatc cggaagcgat gagagtaccg cggtcactac gtgccttggc gagggcttca
      961 ttcttcgtgc ttttagaaac taaataggca atcaagccgc cgaacagcag gggtgtatta
     1021 agctgaatcg gcaaataaat tccgagagcg aaagctaacg acggcacacg aataatttcc
     1081 aggataattg ccaaaaatac tccggctaaa taaagaaccc aaggtgcttg ctccccggtc
     1141 atcaatggtt taataacggc cgccatcgcg gaagcctgtg gggcttgaag cggcgaatcg
     1201 ccaccaaaac caaaagtttt attcagtaat aaaattacca atccgaccga agccgccgaa
     1261 aaaagcgaac cgaggaattt ataactctcc tgcttgattg gcgtagtacc gagccagtaa
     1321 ccaatcttca agtcagtgat aaagccaccc gccatagata aagccgtgca cactactccg
     1381 ccgatcaaga gcgccgccag catgccatga tcgccggtca gaccgacttt tactaaaata
     1441 aatgatgaca gcaaaagcgt cataagagtc ataccggaaa ccgggttagt accgactatg
     1501 gcaatcgctc gggcggcaac cgtagtgaat agaaatgaaa tcactgccac gattagtaaa
     1561 ccgacgatcg cctgtttcag cgtaatccgg acaccgaata aaaagaagaa aaagatggag
     1621 atcaatgtca gaaccaaaaa aaccgtgacc catttcatcg ggatgtcttt ctgggtgcgg
     1681 gaagtttctt caacagtacc gcctttagag ccgccgaata gttctttacc ggctaatttg
     1741 aaagcgccgc cgatgatttt agaagatttt agaatgccga taataccggc ggccgcgatt
     1801 gccccaattc caatcggccg cacataaccg aagaaaatcg catccggagt ttgggcgcca
     1861 agtccggcga cataactcac tatcggcacg attacccacc aggctaaaaa tgaaccgctg
     1921 gcgatgatcg ctgaatagcg taaacctaca atatacccca gaccggttac cgccgcaccg
     1981 atattaatct ttaaaaccca ttttaattta tcagccagca aagcccccca gcgaaaaacc
     2041 gaggtcgaga ccacttcttt ccaaaaacct aatccagcta cgcaaaagtc atatagtcca
     2101 ccgacaagcg ccgcggatac cagcactttt gcctgagcgc caccgccctc tccggcaatc
     2161 agcacttccg tcgtcgccgt cgcttccggg aagggaaatt cgccgtgcat atccttgacg
     2221 aaatatttac ggaaaggaat cagaaataga attcccagaa agccacccaa aagagacgct
     2281 aagaaaatct gaagatagct ggcatctaag ccgagtatat aaagccccgg aattgtgaaa
     2341 atcgctcccg ccacgattac ccctgaactg gaaccgatgg actgaataat aacgttttcc
     2401 tgcagggcat ttttacgctt taaaagaacc gacgtcccag ccgccagaat ggcaatcgga
     2461 atagccgctt cgaatacctg accgattttc agtcccaaat atgccgccgc catcgaaaat
     2521 aacacagcgt aaaaaagtcc ccacccgagc gagtagggag taacttccgg gacgattttt
     2581 gcatcgggaa ttaccggtat atattcttca ccaggcttga gaggacgata agcattttcc
     2641 ggtaatttgg taacagtcga atcctttgac tccattattc ccccgctatt tttatggtta
     2701 agtgtatgtg gattgcgatg cgatcataaa gatcggctaa tgtgattttt cctcaatata
     2761 attcttgacc tggtcggata actcggcgaa aagccgatca atctcttcat ctgataaaga
     2821 ctccaaccag tcgcggactt tgtgaccgta tttttgagtc cattcgaaat aagccatttt
     2881 accgacgtca cgtccctgat ccttactaca tatccatttg tacttttcca tctcggaaat
     2941 ctggatgccc atttccttct catgacgtga gagatttttg ctgaaatcta ccttatcggt
     3001 tttagaggac ataattttat ctgttttttc gatgtctatc ataattaacc ggcaaatata
     3061 gaacaactgg aggttaattg caaac
//
LOCUS       DAOSMP010000118         3053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_32115_length_3053_cov_13.880587, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000118 DAOSMP010000000
VERSION     DAOSMP010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32115_length_3053_cov_13.880587"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PKX09_10110"
     CDS             complement(<1..469)
                     /locus_tag="PKX09_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HOU18215.1"
                     /translation="MAEQEAKKYPLLENINSPADLRKLSVDELVSLSTELREYIVETI
                     TNTGGHLASSLGAVDLTIALHYVFDTPVDKLVWDVGHQAYGHKILTGRREAIKTIRQY
                     GGISGFCKMSESEYDCFGAGHASTSISAAVGMAVARDLNHENYRIAAIIGDGAF"
     gene            complement(469..1038)
                     /gene="pdxT"
                     /locus_tag="PKX09_10115"
     CDS             complement(469..1038)
                     /gene="pdxT"
                     /locus_tag="PKX09_10115"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HOU18216.1"
                     /translation="MNNKAKRVGILGLQGAFQKHLEIINSLGAEAIVIRYKEQIEKCD
                     GLIIPGGESTTMSRLIDEMDLRESLEKFPGMIFGTCAGAILLSTNGTDPLVKSLGRSS
                     VKVKRNAYGRQVESFVENVDLTFSDKPFKGIFIRAPKLDHLDSSVEILGHLNGDVVLA
                     RSGNNLMATFHPELTDDFRIHQYFLNHLQ"
     gene            complement(1072..1953)
                     /gene="pdxS"
                     /locus_tag="PKX09_10120"
     CDS             complement(1072..1953)
                     /gene="pdxS"
                     /locus_tag="PKX09_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOU18217.1"
                     /translation="MDKGSFEVKVGLAEMLKGGVIMDVTTPEQAKIAEEAGAVAVMAL
                     ERIPADIRAAGGIARMSNPKVIKAIQDTVSIPVMAKCRIGHFAEAQVLEALGIDFIDE
                     SEVLTPADEENHIWKHDFKVPFVCGCRDLGEALRRIGEGAALIRTKGEAGSGNIVEAV
                     RHMRRVQSEIRKLTTLSDDELMAEAKRLGAPFELVRQIAKTGKLPVPNFAAGGIATPA
                     DASLMMQLGAESVFVGSGIFKSEDPAARARAIVLATTYYNNPEKIAEVSAGLGEAMRG
                     LDLAEIPENERLAKRGW"
     gene            complement(1997..2836)
                     /gene="ispH"
                     /locus_tag="PKX09_10125"
     CDS             complement(1997..2836)
                     /gene="ispH"
                     /locus_tag="PKX09_10125"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOU18218.1"
                     /translation="MALKVNISATVGFCAGVQNAVKKALTAAQKFGRVAMLGDIVHNE
                     KVIRELTDLGVEVVQNLDQIEPTVPVLFRSHGTPRSIWEEAHQRGLRIIDATCPLVRK
                     IHNEVQMLAKEGRQVIIIGDKNHEEVSGIASQINDPIIVSSPEEAERLLKMKKIGVVA
                     QSTQMIENVSAIVAILAAKTDDLRFMNTICRPTRQRQEQIRELALANDVMLVVGSRNS
                     ANTRRLAEIAKKLNTNTYHIENAEQINPDWFINKTSVGVATGASTPVEVVEEVVKKLT
                     NFD"
     gene            complement(2836..>3053)
                     /locus_tag="PKX09_10130"
     CDS             complement(2836..>3053)
                     /locus_tag="PKX09_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOU18219.1"
                     /translation="FVNVTPLEPEWEGFVTISIANLAPVPVKIYANEGVAQVIFLESD
                     EACRISYADKKGKYQAQRNITNAKIKE"
BASE COUNT          786 a          778 c          616 g          873 t
ORIGIN      
        1 taaatgctcc atctcctatt atagccgcaa ttcgatagtt ttcatgattc aaatcacgag
       61 caaccgccat tccaaccgcc gcggaaatcg aggtgctagc gtgtccggcc ccgaaacagt
      121 catattcgct ttcggacatt ttacagaagc cgctaatacc accatattgg cgaatagttt
      181 taatggcttc tcgacgacca gtcaatattt tatgtccgta tgcctgatgc ccaacatccc
      241 agactaattt gtcaaccggc gtatcgaaaa cataatggag agcaatggtc aaatcaacag
      301 cccccagact cgacgctaaa tgtccaccgg tattagtaat agtttctaca atatattcac
      361 gcagttcggt acttaaactt accagttcgt caactgataa ttttctcaaa tcagccgggg
      421 aattgatatt ctccagtaag ggatattttt tcgcttcttg ttccgccatt attgtaagtg
      481 attgagaaaa tattgatgaa tacgaaaatc gtcggtcaat tccggatgaa aagttgccat
      541 aagattattg cctgaccggg cgagaaccac atcgccattt aaatggccaa gaatttcaac
      601 agaagaatcg agatgatcga gtttcggagc gcgaatgaaa atgcctttga agggtttgtc
      661 ggaaaacgta agatcaacat tttcaacaaa tgattctacc tgccgtccgt aggcatttcg
      721 cttaacttta accgaactac gtcctaatga tttgaccaat ggatccgtgc cgtttgttga
      781 tagcaaaatt gctccggcgc aggtaccaaa aatcatcccg ggaaatttct caagactttc
      841 gcgcaaatcc atttcgtcaa ttagccttga catcgtggtt gattcgccac cgggaattat
      901 caaaccgtca catttttcga tttgttcttt atagcgaata acgatggctt cggctccgag
      961 ggaattgata atttcgaggt gtttctgaaa agcgccctga agtcccaata tgccaactct
     1021 tttggcttta ttattcataa gatagtgaga ttcaatatac tatttctatt tttaccaacc
     1081 gcgttttgcg agtcgttcat tttccggaat ctcggcgaga tcaagcccac gcatagcttc
     1141 tcccaatccg gccgaaactt cagcgatttt ttcgggatta ttataataag ttgtcgctaa
     1201 aacgatagct cgagcccggg cggctgggtc ttcggatttg aaaattcccg aaccaacgaa
     1261 cacactctcc gctcccaatt gcatcattaa agaagcatca gccggggtag cgattcctcc
     1321 cgccgcaaaa ttcggcaccg gtaattttcc ggttttagca atctgcctaa ccaattcaaa
     1381 gggagcgccc agacgtttgg cttccgccat taattcgtca tccgacaaag tcgtcagttt
     1441 acgaatttcg gattgcaccc gacgcatatg acgcaccgcc tcgacgatat tgccactacc
     1501 ggcttcgcct ttggtgcgaa tcaaagccgc tccttcgcca atgcggcgca atgcttcacc
     1561 tagatcgcga caaccgcaaa cgaacggcac cttgaaatcg tgcttccaaa tgtggttttc
     1621 ttcgtcagcc ggggttaaaa cttcactctc atcaataaaa tcaatcccca gcgcttccag
     1681 tacctgcgct tccgcaaaat ggccaatgcg acatttcgcc atcaccggga ttgagactgt
     1741 atcctgaatc gctttaatta ctttgggatt cgacatacgg gcaattcccc ctgccgctcg
     1801 aatatcggct ggaatccttt cgagtgccat cacagcgacg gctcccgcct cttcggcaat
     1861 cttggcttgc tcgggcgttg ttacatccat tatcacgccg ccttttagca tttcagccaa
     1921 gccaactttt acttcaaaag aacctttatc cattttgaat aattactcct tttcatacga
     1981 ttaaatttaa gacatttcag tcaaaattgg ttaatttttt aaccacctct tccaccactt
     2041 caacaggcgt cgaggcccca gtcgccacgc cgactgatgt cttattaata aaccagtccg
     2101 gattgatttg ctcggcattt tcaatgtggt aagtattggt attcaacttt ttggcaattt
     2161 ctgccagcct ccgtgtattg gcactatttc ttgagcccac aaccagcata acgtcattgg
     2221 ctaaagcgag ttcccgaatt tgttcctgac gttgacgagt tggacggcag atagtattca
     2281 taaagcgcag gtcgtcggtt ttagccgcca gaatcgcgac gattgccgat acattttcaa
     2341 tcatctgagt ggattgcgct acaaccccaa tttttttcat ctttagtaaa cgttcggcct
     2401 cctccggact cgaaacgatg ataggatcgt tgatttgact ggcaatgccg ctgacttctt
     2461 cgtgattttt atctccgata attatcacct gacgcccttc tttggctaac atttggactt
     2521 cgttgtgaat tttacgaact aaaggacagg tcgcatcaat aattctcaag cctcgttgat
     2581 gcgcttcctc ccagatggat cgaggcgtac cgtgcgagcg aaataaaact ggtacggttg
     2641 gttcgatctg gtcaaggttt tgtactactt cgacacccag atcggttaat tcacgaatca
     2701 ctttttcatt gtgaactatg tcaccaagca tcgccacccg accaaatttt tgagccgcgg
     2761 ttagcgcctt ctttactgcg ttttgtactc cggcacaaaa accgaccgta gccgagatat
     2821 tgacttttaa agccattatt ccttgatctt cgcgttggta atgtttcgct gggcttgata
     2881 cttccccttt ttatcggcgt acgaaatccg acaagcctcg tcgctttcca aaaaaataac
     2941 ctgcgcgaca ccttcgttgg catagatttt caccggtacc ggtgcgagat tggcaatgga
     3001 aatggtaaca aaaccctccc attccggctc aagcggggtt acattaacga aaa
//
LOCUS       DAOSMP010000119         3049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_32172_length_3049_cov_17.361055, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000119 DAOSMP010000000
VERSION     DAOSMP010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32172_length_3049_cov_17.361055"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PKX09_10135"
     CDS             complement(<1..539)
                     /locus_tag="PKX09_10135"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU18220.1"
                     /translation="MSNAYLGELAALLVAICWSFSSLSFEAAGKRIGSLQVNFIRLVL
                     AFIFLTLFNGIFRHQFWPRSFSAHAWFWLSLSGLVGFVFGDYFLFNAYVIVGARVSML
                     VMSLVPLITAVIGWLFLGEVMNLREILAMLVTICGVAIVIVGRNTENGKLKFSQPATG
                     ILYALGGAIGQAVGLVLSK"
     gene            complement(616..2898)
                     /locus_tag="PKX09_10140"
     CDS             complement(616..2898)
                     /locus_tag="PKX09_10140"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOU18221.1"
                     /translation="MNLNYLFLIAPIGSIIALVTAWIFYKLMLKNDPGNDQMQKIALA
                     VREGAFAYLKQQYKVVALFFLIIFVLFLVMSFGLNVQNKWVPFSFLTGGFFSALAGYF
                     GMSTATLASNRTAQGARKSLNAGLQIAFRSGAVMGLVVVGLGLLDISMWFAILYWLVP
                     SLGFDQMSLAAITTTMLTFGMGASLQALFARVGGGIFTKAADVGADLVGKVEAGIPED
                     DPRNPATIADNVGDNVGDVAGMGADLYESYCGSILATAALGVAAFQGQPHQKILAALL
                     APMIIAGVGNIFSVIGAFAVRTEEKADQRTLLKALSRGVNLSVVLIAIFTAILMYFLK
                     LPMGIVASVITGLLVGWVIGKITEYYTSQSYKPTRWIAEQSKTGPATVIIAGLSTGML
                     STGLPVLTAGIGIILSYAFAGGFGADPNHINLGLYGISFAAVGMLSTLGITLATDAYG
                     PIADNAGGNAEMSGLPKEVRQRTDALDSLGNTTAATGKGFAIGSAALTAMALIAAYIE
                     EIKAQFSHIINNPAILQNLLSEVNIQFDPGASLKTLQELVANNLHLPQWIEYYNVTLM
                     NPRVLVGMFLGSMVTFVFAALAMKAVGRAAGNMVEEVRRQFREKPGIMEGKDEPDYAS
                     CVAISTKGAQREMILPSLLVITVPVLTGLILGVAGVLGVLAGGLTTGFVLAVMLNNSG
                     GAWDNAKKYIEEGNLGGKGSDAHKAAVVGDTVGDPCKDTAGPCLNIVIKLMSTVSIVT
                     VGLAVKFGPVIGTWLGIGPR"
BASE COUNT          925 a          799 c          613 g          712 t
ORIGIN      
        1 tatttgctaa gaaccaagcc gacagcctgt ccgattgcgc ctcctaaagc gtatagaatt
       61 ccggtcgccg gttgcgaaaa ttttaatttg ccgttttcag tattccgccc gacaattaca
      121 attgccaccc cgcaaatcgt cacgagcatt gccagtattt cccttagatt catcacttct
      181 ccgagaaaca accagccgat cacagccgtt atcagcggta ctaatgacat taccagcatt
      241 gaaacgcgtg ctccgacaat tacgtaagca ttgaataaaa aataatcgcc aaatacgaag
      301 ccaaccaggc cggacaaaga aagccagaac caggcatggg cgctgaacga tcgcggccaa
      361 aactgatgcc taaaaatacc attgaacagc gtcaggaaaa taaatgccag tactaagcgt
      421 atgaaattaa cctgtaggga accgatccgc ttaccagccg cctcgaatga aagtgaagaa
      481 aaagaccagc atatagcaac cagcagagcg gctaattctc ccaaataggc attactcatc
      541 gcgttcggca tcaaatttaa aaataaaaaa atcggcgcaa tcttcggaga ctacgccgat
      601 ttttaaaatg ttcaattatc tgggaccaat tcccaaccag gttccgataa ccggtccgaa
      661 cttgactgct aatccgacgg tcacgatcga aactgttgac atcaatttaa ttacgatatt
      721 gagacatgga ccggcagtat ccttgcaagg atcgccgacc gtatcgccca ctactgcggc
      781 tttatgtgca tcggaacctt tgccgcctaa attaccttcc tcgatatatt tcttggcgtt
      841 atcccaggca ccgcccgaat tgttcagcat aaccgccagc acaaaaccag tcgtcaaacc
      901 accggctaat acacccaata cgccggcaac tcccaaaatt agaccggtca aaaccggtac
      961 agtaatcact aacagtgatg gtaaaatcat ttcgcgctga gcgcctttgg tgctgattgc
     1021 tacgcaactg gcgtaatcgg gttcgtcttt gccttccata attcccggct tttcacggaa
     1081 ttgacggcgc acttcctcga ccatattacc agcggcacga ccaaccgctt tcatcgctaa
     1141 agccgcaaaa acgaaggtca ccattgagcc gagaaacatt cctaccagaa cgcgcggatt
     1201 cattaaagtt acattataat attcaatcca ttgcggtagg tgcagattgt tcgccaccaa
     1261 ctcctgcaag gtcttcagtg aagcgccggg atcgaactga atatttactt cactcagcaa
     1321 attctgtaag atcgccggat tattgataat gtgggaaaat tgagctttaa tctcttcaat
     1381 ataagcggca atcaaagcca tagccgtcaa agcggctgag ccaatggcaa agcccttgcc
     1441 ggtcgcggca gtagtattac ccagcgaatc cagagcgtcc gtacgctggc gcacttcttt
     1501 cggcaatccg ctcatttcgg cattgccacc ggcattatcg gcaatcggtc cgtaagcgtc
     1561 ggtcgccaag gtaattccca atgtagagag catcccaact gccgcaaagc tgattccata
     1621 aagcccaaga tttatgtgat tcggatcagc accaaatcca cccgcaaaag cataactcaa
     1681 gataattccg ataccggcgg tcagaaccgg taatcccgtc gaaagcatac cggtcgaaag
     1741 cccggcaata atgactgtcg ccggaccggt tttggactgt tcagcgatcc agcgcgtcgg
     1801 tttataactt tgcgaagtat agtactcagt tatttttcca attacccagc caactaataa
     1861 tcccgttata acggaagcca cgatgcccat cggcaatttc agaaagtaca ttaaaattgc
     1921 agtaaatatc gcaattaaaa cgacacttag attgactccg cgggaaagcg ctttcaataa
     1981 tgttcgctga tcggcttttt cttcggtgcg gacggcaaag gcgccgatca cagaaaaaat
     2041 gttgccaact ccagcaatta tcattggggc taaaagtgcc gccaaaattt tttgatgcgg
     2101 ctgtccctga aaagcggcta ctccgagcgc cgctgtggct aaaatcgaac cgcaataaga
     2161 ttcataaaga tcggcgccca ttcccgcgac atctccgacg ttgtcgccga cgttgtcggc
     2221 aatcgtggct ggattgcggg ggtcatcttc cggaattccg gcttcaactt tgccaaccaa
     2281 atctgctccg acgtcagcgg ctttagtaaa aataccaccg cctactcgag caaacaacgc
     2341 ctgtaaagaa gcgcccatcc caaatgtcaa catcgtggtc gtaatcgccg ccagactcat
     2401 ctggtcgaat cccagacttg gtaccaacca gtatagaatc gcaaaccaca tcgagatatc
     2461 taacaatccc aaacccacaa ctactaagcc cattacggca ccggaacgaa aggcaatttg
     2521 aagtccggcg ttcagtgatt tacgagcacc ttgggcagta cgattggatg ccagtgttgc
     2581 cgtactcatt ccgaaatatc cagctaaagc cgaaaagaaa ccacccgtca agaatgaaaa
     2641 tggcacccat ttattctgaa cattaagtcc gaatgacata accagaaata acacaaaaat
     2701 aatgagaaag aataatgcca ccactttata ttgctgttta aggtaggcaa aagcgccctc
     2761 gcggactgct aaggcaattt tctgcatttg gtcattaccc ggatcgtttt tcaacatcag
     2821 tttataaaaa atccaggcgg taaccagggc aatgatcgaa ccaattggtg caattaaaaa
     2881 taaatagttt agattcattc ttaacaaacc tcctttaagt tgtgactaaa atcgtgcgga
     2941 cccaatccga ccccaaatta cttttactga aaattatcat cattaaaata actcggaaat
     3001 atataactat ttcagctaat tctcaaaaaa ttatctgaaa aataaacca
//
LOCUS       DAOSMP010000120         3029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_32457_length_3029_cov_13.225622, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000120 DAOSMP010000000
VERSION     DAOSMP010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32457_length_3029_cov_13.225622"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PKX09_10145"
     CDS             complement(<1..346)
                     /locus_tag="PKX09_10145"
                     /inference="COORDINATES: protein motif:HMM:NF026853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvaI/BcnI family restriction endonuclease"
                     /protein_id="HOU18222.1"
                     /translation="MEKYGYKDDRGRQALYNIVNKKTPNSQGFYLISDPERHLIILKN
                     IYEDVNIAEWSTYVIAGKFMTKLDRLLLIIADNKIENGKEYFHFNEAYLLENPTPERF
                     LLAFEKNELMIDL"
     gene            complement(229..633)
                     /locus_tag="PKX09_10150"
     CDS             complement(229..633)
                     /locus_tag="PKX09_10150"
                     /inference="COORDINATES: protein motif:HMM:NF026853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvaI/BcnI family restriction endonuclease"
                     /protein_id="HOU18223.1"
                     /translation="MQLKELIKKLEALEQKGYLETSRQGPTGIGHLLEKEMGLFENNI
                     PIPDIGGRVELKATRRNVNSLITLFTFNQKNSPMLASGYEIRGFGLSNKTILSKNMDT
                     KMIEVDKHYITLLIKKRRIPRDFTSFQILKGT"
     gene            complement(635..1963)
                     /locus_tag="PKX09_10155"
     CDS             complement(635..1963)
                     /locus_tag="PKX09_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOU18224.1"
                     /translation="MENINKNKQVVKIVNEPDLEYQIKIYSRNAKIVDDSWDFRNSNT
                     KDFTHCFHSYPAMMIPQVARRIIDNLGKKSKILFDPYCGTGTSLVEANLKGIDAIGTD
                     LNPLARLIATAKTTKIDIKILDLFLQDFKDYIFSIDFDIGKIYSEIIPDINNIDYWFS
                     KVTQQKLSLILKYIDNISEFSIKNFFKVAFSETVRESSYVKPGEFKLVRSKNYLDKSE
                     PDVFRMMLSKLLRNRIGLIEFIECCKNNAKTSVYDFNTINEIPNAILPTEIVDIVVTS
                     PPYGDSRTTVAYGQYSRLANEWLGFKDANKIDRILMGGEKKKHIPVFKSGILNDVIDT
                     IQKEDKERALDVISFYEDYEKSIKNVSATIKKGGFACYVVGNRTVKGLTIPNDEITAQ
                     LFEENNFSHIETIVRNIPNKRMPLKNSPSNVVGRTASTMKNEYIVIMAKN"
     gene            complement(2308..>3029)
                     /locus_tag="PKX09_10160"
     CDS             complement(2308..>3029)
                     /locus_tag="PKX09_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOU18225.1"
                     /translation="TCGKCKVMTSQERVNIMVKGLGDMCRTMDVFIIGGGFSTSFSDI
                     VPAVVVSVIGKLVTEKPLTKPAKNVGDKIIIIGVTGNDGNDTLFRAGLVEEMRPAIAL
                     FKEEKTAMEAALAAFATGKINACSDLGAAGIGAAICESARYGRRGAKVDLSQVPVSVK
                     DITPQEILICETQARYVVQVAPESVDEVLKAIRTKTEHVAVIGEITDTQQEVFEYKGE
                     IIATIPNQPSPETLAILKSGK"
BASE COUNT          886 a          588 c          486 g         1069 t
ORIGIN      
        1 gtaaatcaat catcaattca tttttttcaa atgctaataa aaacctttct ggagttggat
       61 tttccagcag ataagcctca ttaaagtgga agtattcttt tccattttct attttattgt
      121 cggcaattat taaaagtaat ctatcaagct tggtcatgaa tttaccagca attacatagg
      181 tgctccactc cgcaatattc acatcttcat agatgttttt taagatgatc aagtgccttt
      241 caggatctga aatgaggtaa aatccctggg aattcggcgt ttttttatta acaatgttat
      301 atagtgcttg tctacctcta tcatctttgt atccatattt ttcgataata ttgttttgtt
      361 tgacaagcca aaccccctta tttcgtaccc cgacgcaagc atcggggaat tcttttgatt
      421 aaaagtaaaa agtgttatta gcgagtttac attccgtcta gtagctttta gttcaactct
      481 acccccaata tctggaatag gaatattgtt ttcaaataat cccatttctt tttctagtag
      541 atgcccaata ccagttggtc cttgtcttga agtttcaaga tatcctttct gctcaagggc
      601 ttctaatttt tttattagtt cttttaactg catatcaatt ttttgccata ataactatat
      661 attcattttt cattgttgaa gcggtcctgc caacgacatt agaagggcta ttttttaaag
      721 gcatcctttt attaggtata ttcctaacaa tagtttcaat atgactgaaa ttattttctt
      781 caaataactg agctgttatt tcatcgttag gaatagtaag acctttaact gttcggttgc
      841 ccacaacata acaagcaaaa ccaccctttt ttatggtagc tgaaacattt ttaattgatt
      901 tctcataatc ttcataaaag gaaattacat caagagctct ttctttatct tctttctgaa
      961 ttgtatctat tacatcgttt aatattccac ttttaaatac gggaatatgc ttcttcttct
     1021 ccccacccat taaaattcta tctattttat ttgcatcttt aaaaccaagc cattcatttg
     1081 ctaacctaga gtattgccca taggcaactg tagtgcgtga atcgccgtag ggaggagatg
     1141 tcacaacaat atctacaatc tctgttggaa gaatagcatt tggaatttca tttattgtat
     1201 tgaaatcata taccgacgtc ttagcgttat ttttacaaca ctctataaat tcgataagtc
     1261 caattctatt tcttaataat tttgaaagca tcattctaaa aacgtcaggt tcagatttat
     1321 ctaaataatt tttgctcctg actaatttga actctcccgg ttttacgtat gaagactcgc
     1381 gaactgtttc agagaaagca actttaaaaa agtttttaat tgaaaattcg ctaatattat
     1441 caatgtattt tagtatcaat gatagttttt gctgagtaac ttttgagaac caataatcta
     1501 tattgtttat atctggaata atttccgaat atattttgcc aatatcaaag tctatagaaa
     1561 aaatgtaatc cttaaaatcc tgaagaaata agtcaagtat tttgatatcg atctttgtag
     1621 ttttagccgt ggcgattaac ctcgccaaag gattcaaatc agtaccgata gcatctatac
     1681 cttttagatt agcttcaact aaagacgtcc ctgtcccaca ataaggatcg aataagattt
     1741 ttgatttctt acccaaatta tctataatcc ttctagcaac ttggggaatc atcattgctg
     1801 gatatgagtg aaaacaatgc gtaaaatctt tggtatttga atttctaaaa tcccaactat
     1861 catccacaat ctttgcattt cgagaatata tttttatttg gtattctaaa tcaggttcat
     1921 ttacaatttt tactacctgt ttatttttat taatattttc cattgtatta gagctagtta
     1981 gacctcatat ttattacaaa ctacctattg cataaaattt tgatcatttg ctaaaaatta
     2041 agtcatcaaa ggtaatttgt caacgacaat taaagatcaa gctatttaat aatttttagg
     2101 atatttaggg tatcttgtgg ggggatctga ttttgttttc tgtaattacc acatagccat
     2161 gtctaatatt cagagatttt tgtacctttt aagataaact cataatccaa atatttatat
     2221 tgacattccg agatgaaaat tcaagattgc cactctcacc ttcttaaagc cggtaactgt
     2281 ttatctaaat ttcttatcgg gttttattta tttgccactt ttcaaaattg ctaaagtttc
     2341 gggtgagggt tgattgggaa tcgtggcaat aatctcacct ttgtattcga aaacttcctg
     2401 ctgggtatcg gtgatttctc cgattacggc gacgtgctcg gttttggtac gaatggcttt
     2461 tagcacttca tctactgatt ccggcgcaac ctgaacgaca tagcgtgcct gggtctcgca
     2521 aattaatatt tcctgcggag taatatcttt aacggaaacc ggcacctggg acaaatcaac
     2581 tttagcaccg cgccggccgt aacgggctga ttcgcaaatt gccgcaccaa tgccggcggc
     2641 acccagatca gaacaggcat taatcttgcc agtcgcaaaa gccgccaagg cggcttccat
     2701 cgcggttttt tcttctttaa atagggcaat agccgggcgc atttcttcta ccaatcccgc
     2761 ccggaatagg gtgtcgttgc cgtcattgcc ggtcacgcca ataatgataa ttttatcacc
     2821 gacatttttg gctggtttgg taagtggttt ttcggtaacc agtttgccga taaccgagac
     2881 gacgacagcc ggtacgatat cggagaaaga agtagagaaa ccgccgccaa tgataaaaac
     2941 atccatcgtt cggcacatat cccccaaacc ttttaccata atattcactc tttcctgact
     3001 ggtcataacc ttacatttgc cacaggtgc
//
LOCUS       DAOSMP010000121         2994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_32939_length_2994_cov_9.317455, whole genome shotgun sequence.
ACCESSION   DAOSMP010000121 DAOSMP010000000
VERSION     DAOSMP010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32939_length_2994_cov_9.317455"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            429..2603
                     /locus_tag="PKX09_10165"
     CDS             429..2603
                     /locus_tag="PKX09_10165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU18226.1"
                     /translation="MKIVHLLTLMILSLNFAYADWNQTSGPCGLKIQSFYVKDDRLFA
                     ITDQSIYLSRDKGLSWSRIYEEPTTNLNISAFTAVGQHLFARVFSTIIHSDNNGESWV
                     EYPNDISGYPISNFNSYDTVLFATTYEAGIFRSIDCGSSWEPINNGLEDLTINYFTIK
                     DSLLFARNGDGIFISTDLGNSWSLFPTPSRNLGSLNITGNNIYMFNSYRIIHSVDWGN
                     TWTSLENMPDMDYNVMRAAGSYIFLGAADDYISFIGFCYSPDYGENWIIPDNNGLTNR
                     YISAMAIIDSNIIVGTDGGGVYISSDCGMNWIESNVGFKGTSPVCVLSLAAIDNNIIA
                     GTWGGGIFLSRDKGANWSEINVGLREKNILSLAIVKNRIFAATAHCGIYCSENGGATW
                     YAVNNGIPNTGKSSFQISFAVRDTILFTGIWETGIYRTNDYGQNWVSVGDGLSQEVIN
                     SIYCLVADSQSIYIGGYGIYYSNDDGQNWKAMNDGLDLENRSLIVTINIRDTTFFAGD
                     ISYGVFRSTKNNPKWIPVNNGLPMSSFGFIYRFIKNDITCMASIGQYLLVGLLDRGIF
                     LTEDNGNNWIEVSEELSNPPVYSMLINDSDVFVGLYGNVGQYTEDNNNGGILCCPIED
                     LVKKLAICKEQLPSGFLLYQNYPNPFNPITNISFSIPIESQVTLKIFDIIGKEVAVLV
                     AGLFPAGKYTTQWNSNGLPSGIYLYQLQTGNFSQTNKLLLLK"
BASE COUNT          937 a          437 c          573 g         1047 t
ORIGIN      
        1 ctatcttgcc aaaaaagcgc ccaaagttag gtaaaattat ttaatttttc acgtgaaatt
       61 ttgaggattt attaaaaata ttgcaaagat aaattgcatt atcctggccg ccaagcggtg
      121 gaaccaattc caaagaccat ttactttgaa atagaatgta gatcttagag tattttagaa
      181 attatctcta tcataaaaat actacttcgt tgaggttaaa taatccaaag atattaaggt
      241 cagcccggct gtaattgttt gcctttttat ttgaattata ctatcttaca aatgatttta
      301 tattgtgagc attgattttt tcatagggag ttaatgaaaa aggaactcaa gagttataat
      361 agtaagattt atggtaattg gtttcttatt tattttaaat ttaatcaata taataggggg
      421 gaataattat gaaaatcgtt catctcctta ctcttatgat tttatcatta aattttgcat
      481 atgccgattg gaatcaaact agtgggccct gtggcctaaa aattcagtcc ttttatgtta
      541 aagatgatcg tctttttgct attaccgatc aaagtattta tttatctagg gataaaggct
      601 taagttggtc aagaatttat gaggagccga ccactaactt gaatattagt gcttttacag
      661 cagtaggtca acacttgttc gcgcgtgtct tttctaccat aattcattca gacaacaacg
      721 gtgaaagttg ggtggaatat cctaatgata tatctggtta tccgatatcg aattttaatt
      781 cttatgatac cgttctattc gccacaacgt acgaagcagg gatttttaga tcaatcgatt
      841 gtggcagctc ttgggaacct attaataatg gtttagaaga tttaacgatt aattatttta
      901 ctataaaaga ctcattactc ttcgcaagaa atggagatgg aatttttata tcaaccgatc
      961 taggaaattc atggtcttta tttccaacac ccagtcggaa cttaggtagt cttaacatta
     1021 ctggtaataa tatatatatg tttaattcct atcgaataat tcattctgtt gactggggaa
     1081 acacatggac tagtttggaa aatatgcctg atatggatta taatgttatg agagcagctg
     1141 gttcgtatat ttttcttggg gcagccgatg attatattag ttttatagga ttttgttatt
     1201 ctccggatta tggtgagaat tggataattc ctgataataa tggactaact aatagatata
     1261 tatcagcaat ggcaataatt gactcaaaca taatagtagg gacagatggt ggaggtgtgt
     1321 atatatcttc tgattgcgga atgaactgga ttgaatctaa cgttggtttt aaaggtactt
     1381 ctccagtctg tgttttaagt cttgccgcga ttgataataa tattatcgct ggaacgtggg
     1441 gtggaggtat ttttctttcc cgcgataagg gggccaattg gagtgaaatt aatgttggtc
     1501 ttagagaaaa aaatatttta tctctagcta tagttaagaa tagaatattt gcggcgaccg
     1561 cacattgtgg catatattgt tctgaaaacg gtggtgctac ctggtacgcg gttaataatg
     1621 gcataccaaa tactgggaaa agtagttttc aaatttcctt tgctgttcgg gacacgattt
     1681 tatttacagg aatttgggaa actggtattt atcgtactaa tgattatggc caaaactggg
     1741 tatcagttgg cgatggtttg tcacaagagg taattaactc aatctattgt cttgtcgccg
     1801 atagtcaaag catatatatc ggcgggtatg gtatctatta ttccaatgat gatgggcaga
     1861 attggaaggc aatgaatgat ggtttagact tggaaaatcg aagcttaatt gtgactataa
     1921 atattagaga tactactttt tttgcgggag atatttcata tggggtattt cgctcaacca
     1981 aaaacaatcc taaatggatt ccggttaaca atggtttacc gatgagtagt ttcggattta
     2041 tttatagatt tattaaaaat gatataacct gcatggctag tattggacag tatcttttag
     2101 ttgggctgct cgatcgtggc atttttctga cagaggataa cggtaataat tggattgaag
     2161 tcagcgaaga attatcaaat ccacctgttt attcaatgct tatcaatgac tcagacgtat
     2221 tcgtagggct ttacgggaat gttggccaat ataccgaaga taataataat ggtggaatat
     2281 tgtgttgccc aatcgaagat ttggttaaaa aattagctat ttgtaaggaa caattgccct
     2341 caggtttttt attatatcaa aattatccga atccgtttaa tccaataacc aatatttcat
     2401 tttctattcc aatcgaatct caagtaactt tgaaaatatt tgatattatt ggtaaagagg
     2461 tggcagtttt ggtggctggg ttattcccgg cggggaaata tacgacgcaa tggaattcca
     2521 atggtttacc aagtggtatt tatctttatc aacttcaaac cggaaatttt tcacaaacta
     2581 ataaattatt attattaaag taaatattga taaaattagg tgctttattt ttattcatat
     2641 tttatatttt aaatcgagta aatactaaaa gcgggtaata taagttcaat tgtctgccga
     2701 ttgatttaac tttttgttac tcaggacatc ccctaatttt tcttagtata atctgctaac
     2761 tcattgataa tccttgtttt attcctatac aagtggccgc acatttttct caaactgaga
     2821 gtagataaat actgagactt ggcttaggac tacacccaga aaaatacctg tctaaaaaat
     2881 tggaggaagt tctattttct tacttccttg atagattaga taaaaatctt tacattcctt
     2941 tccgtgaatg aattaattgc cataatagat gacgaaccgg acattctaca gctg
//
LOCUS       DAOSMP010000122         2890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_34491_length_2890_cov_19.919577, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000122 DAOSMP010000000
VERSION     DAOSMP010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34491_length_2890_cov_19.919577"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="PKX09_10170"
     CDS             complement(<1..1493)
                     /locus_tag="PKX09_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HOU18227.1"
                     /translation="MKPKINFTLGRFLIFLTAAALTIIIYAAHNKPRVHLIGDSTMAD
                     KPLDDNPERGWGQLFPLYFTQEIQIINYARNGRSTKSFIDEGLWANVYNNLKPGDFVF
                     IQFGHNDAKFSDTTRYAPARTTYRANLIRFIAESRDKGAYPILITPVNRRKFNEAGEF
                     VDQHGDYPVVVRELAAEYDAPLIDLHARSWELFSHLGAEKSAELFLRVPAGVYKHYPD
                     GKSDNTHFNRYGAIRICELVIEEMKAIDLPLVKYLKDGPELSDMGVGKVVGLDYFYNH
                     EFKNGNQYHYTWEDRENSGFYELGNLFENCGAFIAEVCAEPTPEELARLSIYIIVDPD
                     TPQETTVPNYISEKAIKTITNWVESGGVLVLLGNDAGNAEFEHFNQLAQNFGIHFNEV
                     SLNRVTNKNYEMGKFDNLPDHPLFENITKIYMKEVASLTLTGNAEPILISDGNVIIAS
                     SKYGQGLVLAIGDPWLYNEYIDNRILPADFANYSAALNFCKWLLNQS"
     gene            complement(1499..>2890)
                     /locus_tag="PKX09_10175"
     CDS             complement(1499..>2890)
                     /locus_tag="PKX09_10175"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HOU18228.1"
                     /translation="WQIIGYAVPHLPDSIYDLPQPGRGIWAPALRYHNGEYWVYYGDP
                     DLGIFMVKAQNPAGPWEPPVLVKQACGWIDPCPFWDDDGQAYLIHAWAKSRVGFNSIL
                     TLHKLSSDGRQILDEGVTVFDGHSTHPTIEGPKFYKRNGYYYIFAPAGGVTNGWQTGL
                     RSKNILGPYEDKIVLEQGTTAINGPHQGAWIETNQGESWFIHFQDKGAYGRILHLQPV
                     EWLNDWPMIGIDLDGNGIGEPVQCFRRPDVDNPISAFQMQTSDEFESNSLGLQWQWRA
                     NFKSEWFTLSARSGYLRLFTVNPAPVCHNLGDLPNILTQKFPAEAFTVTTKLSFHPDS
                     NDDQAGLIIIGNDYAALKVTHRGARYQLTYAECTEIEKGKSEIISETVGLNKPEVFLK
                     ANVGEGAVCTFSYSTDGEKYLPLGRKFQAVKGKWVGSQIGIFATTTPGKISSGYADFD
                     FFRISTMDKIELK"
BASE COUNT          748 a          693 c          674 g          775 t
ORIGIN      
        1 cttgactgat ttaataacca tttacaaaag ttcaaggcgg ctgaataatt cgcgaaatca
       61 gcgggcagta tgcgattgtc aatgtactcg ttatatagcc aagggtcacc gatagccaat
      121 accaatccct gaccgtactt cgaactggcg atgattacat ttccgtcaga aattagaatt
      181 ggctcggcat tccccgttaa tgtcaatgac gcgacttctt tcatataaat tttggtaata
      241 ttctcaaata acggatgatc cggcagattg tcgaatttgc ccatttcgta attcttattg
      301 gtaacccggt tgaggctgac ttcattgaag tgaatcccga aattctgtgc cagctggtta
      361 aaatgttcga actcggcatt gccagcgtca ttgccgagaa ggactaatac gccgccggat
      421 tccacccaat tcgtgatggt cttgattgct ttttcggaaa tataattcgg cacggtggtt
      481 tcttgcggcg tatccggatc aacgatgatg taaatgctaa gtcgagccag ttcctctggc
      541 gtcggttcag cgcatacttc ggcaataaat gccccgcaat tttcgaacaa attacccagt
      601 tcgtaaaatc cggagttctc gcgatcttcc caggtgtaat ggtactgatt gccgtttttg
      661 aattcgtgat tgtaaaagta gtccagaccg acaaccttcc cgacgcccat atccgataac
      721 tccggtccat ctttgagata ctttaccagc ggcagatcaa tggctttcat ttcctcgata
      781 actaattcgc aaatccggat tgcgccgtag cgattgaaat gcgtgttgtc ggattttcca
      841 tcgggataat gtttatagac tcccgctggg acacgcagaa ataattccgc agatttttct
      901 gcacccagat ggctaaatag ttcccaactg cgggcgtgta agtcaatcag aggcgcgtca
      961 tattcagcgg ctaattcgcg gacaacgacc ggataatcac cgtgttgatc aacgaattcg
     1021 cccgcttcat tgaatttgcg gcgattgact ggagtaatca atatcggata ggcgcctttg
     1081 tcgcgtgatt ccgcgatgaa tcggatcaga ttagcgcggt aggttgtccg agcgggagcg
     1141 tagcgggtcg tatcgctgaa cttggcgtca ttatgaccga attggataaa aacaaaatcg
     1201 cctggcttta ggttattata aacattagcc cataatccct cgtcaataaa actcttcgta
     1261 ctgcgaccgt tacgggcgta attgataatc tgaatctcct gcgtaaaata cagcggaaaa
     1321 agctgtcccc agccgcgttc gggattatcg tccagcggtt tgtctgccat ggtcgaatcg
     1381 ccgatcaggt gaaccctagg cttattatgc gcggcatata taatgatcgt cagagcggcg
     1441 gcagttagaa aaattaaaaa cctgcccaat gtaaagttta tctttggctt cataatgttc
     1501 atttcagttc gattttgtcc attgtcgaga tgcgaaagaa gtcaaaatcg gcatagccac
     1561 tggaaatttt ccccggcgta gtcgtagcaa aaataccaat ttgagagccg acccatttgc
     1621 ctttgacggc ttggaatttt cggccgagcg gtaaatattt ttcgccatca gtactgtagc
     1681 taaatgtgca aaccgcacct tcgccgacgt tagcttttag gaatacttcc ggcttattga
     1741 ggccaacggt ttcgctaatt atttccgatt tgcctttctc gatttcggta cattcggcgt
     1801 aagtcagctg gtatctcgca ccgcggtgag ttactttgag tgcggcgtag tcgttgccga
     1861 taataatcag cccagcctga tcgtcgttac tatcaggatg gaaagataac ttcgtggtaa
     1921 ccgtgaacgc ttccgcgggg aatttttgag ttagaatatt gggtaaatcg ccaagattat
     1981 ggcaaacagg cgccggattc acggtaaata atcgcagata accggatcga gccgagagtg
     2041 taaaccattc cgatttaaaa ttcgcgcgcc actgccattg aagcccgaga ctattactct
     2101 cgaattcatc gctggtttgc atctggaacg cagagattgg gttgtcaaca tccggtcggc
     2161 ggaagcattg aaccggttca ccaatcccat tgccgtccag atcaataccg atcatcggcc
     2221 aatcatttag ccattcaaca ggctgaagat gcaagatgcg gccgtaagcc cctttatcct
     2281 gaaaatgaat gaaccaggat tcgccttggt tcgtttcgat ccaagcccct tgatgcggcc
     2341 cgttgatggc tgtagtgccc tgctcgagaa cgattttgtc ttcgtaggga cccaagatat
     2401 tttttgaacg caagccagtc tgccagccgt tcgttacgcc accggctggc gcaaaaatat
     2461 agtaatatcc attacgctta taaaatttag gaccttcgat cgttgggtga gtggaatgtc
     2521 cgtcgaaaac ggtcacgcct tcgtccagaa tctggcgacc gtcggagctc aatttatgca
     2581 aagtcaagat actgttaaat cctacccgac tttttgccca ggcatggatt aaataagcct
     2641 gaccatcatc gtcccaaaaa ggacaaggat caatccaacc acaagcttgt ttgaccagaa
     2701 ctggcggctc ccacgggcca gccggatttt gcgctttgac cataaaaata cctagatcgg
     2761 gatcaccgta atagacccag tactcgccgt tatggtaacg aagagccggc gcccagatgc
     2821 cccttccagg ctgaggtaaa tcatagattg aatcgggcaa atgcgggaca gcatatccaa
     2881 tgatttgcca
//
LOCUS       DAOSMP010000123         2868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_34835_length_2868_cov_14.028084, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000123 DAOSMP010000000
VERSION     DAOSMP010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34835_length_2868_cov_14.028084"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1423)
                     /locus_tag="PKX09_10180"
     CDS             complement(14..1423)
                     /locus_tag="PKX09_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HOU18229.1"
                     /translation="MKKLSPVHRHLRLNLTIIILLGMICTWGIAQTPESGWNQVPAIL
                     EKIVPPEFNARDFNITDYGAIGDGQFDCTQAFAKAIDACHKAGGGRVVVPSGKFLTGA
                     IHLKSNVNLHITGEGNILFSRDIHQYLPVVFTRFEGVECLNYSPFIYAYEQENLAVTG
                     SGTIDGQADNETWWNWVMNKKFGWKEGMPNQEKDRDLLMEMADKNVPVKERIFGEGHY
                     LRPNFIQFYKCKNILIEGVTLKRSPMWEIHPVLCENVTVKKVTVISHGPNNDGCDPES
                     CRNVLIKDCYFDTGDDCIAIKSGRNGDGRRLKAPSENIIIQDCRMKDGHGGVVIGSEM
                     SGNVRNVFAEDCIMDSPNLDRALRIKTNSLRGGIVENIYMRNVTVGVVSDAVIRIYFY
                     YAEGDVGQYTPIVRNIYVENVTSQASDYALFLQGYERSPITNVQLSNCRFNGVKKPNI
                     IKNVEGLKMKDVYINGELQ"
     gene            complement(1462..2691)
                     /locus_tag="PKX09_10185"
     CDS             complement(1462..2691)
                     /locus_tag="PKX09_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOU18230.1"
                     /translation="MKAKDNHKKRFYLSGLILIAFIPLIGRAALNQEIKPTSAWSVRM
                     AESVIQRHPQPWLLESPEKPKWEYTYGLVMKSILDVWEKTGEQKYFQYVESYYEYFID
                     DSGRILTYRLEEYNLDRINAGKPLFRLYAETGKVKYQKALMNLRQQMATHPRTKEGGF
                     WHKKIYPHQMWLDGLYMAGPFLAQYAVVFNEPALFDDVIKQFILIEEKTRDPQTGLLY
                     HGWDESRQQAWANPETGCSPNFWGRAMGWYAMALVDVLDFLPADHHGRPEIIAILKRL
                     ADAVVKVQDPATSVWYQVLDKGGQPGNYLESSASCMFVYSLAKGVRLGYLDPVYLDAV
                     KKGYTGILNQFIEVDSTGLVNINQACAVAGLGGKPYRDGSYDYYVSAKTARNDPKAVG
                     PFILASLEMEMLDEKWK"
BASE COUNT          789 a          693 c          559 g          827 t
ORIGIN      
        1 ctggcgattg atttcattgc agttcgccat tgatatagac gtccttcatc tttaagcctt
       61 ccacattttt aataatgttg ggctttttta ctccgttaaa acgacaatta ctaagttgca
      121 cgttagttat aggcgacctt tcataacctt gtaaaaataa agcataatcg cttgcctggc
      181 tggttacatt ttcgacgtaa atgttacgga ctatcggcgt atactgacca acgtctcctt
      241 cggcatagta gaagtaaatt cgaataactg cgtcactgac aaccccgaca gtgacgttgc
      301 gcatgtagat attctcgacg attccacccc gcagggaatt ggttttaatc cgcaaagctc
      361 gatccaggtt aggactatcc ataatacaat cttccgcaaa aacattccgg acgttgcccg
      421 acatttcact gccgattact acaccaccat gcccgtcttt cattcgacag tcttgaataa
      481 tgatattttc gctcggggct ttgagacgtc gtccgtcgcc attgcgtccg gattttatgg
      541 cgatacaatc atcgccagta tcgaaataac agtctttgat caagacattg cggcaacttt
      601 cggggtcaca gccatcgtta ttaggaccgt ggctgatgac ggtaactttt ttgacggtta
      661 cgttttcaca cagcaccgga tgaatttccc acattggtga tcgcttaagc gtaacgcctt
      721 caatcagaat atttttacac ttataaaact ggataaaatt cggccgtaaa taatggcctt
      781 caccgaagat acgttctttt accggtacat ttttatctgc catttccatc aataaatcac
      841 ggtctttttc ctgattcggc attccttctt tccagccgaa tttcttgttc atcacccaat
      901 tccaccaggt ttcattatca gcctgaccat caatggtacc cgatccggtg acagccagat
      961 tctcttgttc ataggcataa ataaaaggcg aataattcag acattccacc ccttcaaatc
     1021 gggtaaatac gaccggcaaa tattgatgta tgtctcggct gaatagaata tttccttcac
     1081 cggtaatatg cagattgaca ttacttttca gatgaatagc acccgtcaga aatttaccgg
     1141 acggtacgac gacgcgtccg cctccggctt tatgacaggc atcaattgcc ttagcgaaag
     1201 cctgagtgca atcgaattga ccatcaccga tagcgccgta atcagtgatg ttgaaatctc
     1261 tggcattgaa ttcgggggga acgatttttt ccagaattgc tggtacttga ttccaacccg
     1321 attcgggagt ttgagcaatt ccccaggtac aaatcattcc cagcagaatg ataattgtca
     1381 gattcagacg tagatggcga tgaacaggcg ataatttttt cattttgtac tagttacact
     1441 ttcatttatt tattcaaata attatttcca cttttcatcc agcatttcca tttccagact
     1501 tgccagaata aatggcccga cggctttggg atcgttgcgc gcagttttgg cgctcacata
     1561 ataatcgtaa gaaccgtcac ggtaaggttt accgcccagc ccagccactg cgcaagcttg
     1621 gttgatattg actagtccgg tggagtccac ttcgataaac tgattcaaaa tgccggtata
     1681 tcctttctta acagcatcaa gataaaccgg atcgagatag ccgagccgaa cgcctttggc
     1741 taacgaatag acaaacatac aggacgccga ggattccaaa taattacctg gctggccacc
     1801 tttatccaga acttgatacc aaacgctagt tgccggatct tgaactttaa cgacagcatc
     1861 cgctaatcgc tttaaaatag cgatgatttc tggtcgccca tgatgatcag cgggtaaaaa
     1921 atcgagcaca tcaaccagcg ccatcgcgta ccaacccatc gcgcgtcccc aaaaattggg
     1981 agaacaaccg gtttctggat tagcccaggc ttgctgacga ctctcgtccc agccatggta
     2041 gagcaaaccg gtttgtggat cgcgcgtttt ttcttcgatt aaaataaatt gcttgatgac
     2101 atcatcaaat aatgccggct cgttaaagac caccgcatat tgagctaaaa aaggtcctgc
     2161 catatataaa ccatccagcc acatttgatg cggatagatt ttcttgtgcc agaaaccacc
     2221 ttctttagtg cgcggatgag tcgccatttg ttgccgcaaa ttcatcagtg ccttttggta
     2281 tttgaccttg ccggtttcgg catagagtcg aaaaaggggt ttaccagcat tgatccggtc
     2341 aaggttatac tcttccagac gataagttaa aatgcggccg ctatcgtcaa taaaatattc
     2401 ataataggac tcgacatact ggaaatattt ttgttctccg gttttttccc aaacatccag
     2461 aatagatttc ataaccagtc cgtaagtata ttcccattta ggtttctccg gactttcgag
     2521 tagccagggt tgcggatgtc gctggataac cgattccgcc atccgcaccg accaggctga
     2581 ggttggttta atttcttgat tcaatgccgc tcttccaatc agcgggataa aggcgatcag
     2641 aatcaagccc gacaaataaa atcttttctt atggttatct ttagccttca tatttactca
     2701 attggtaaaa atatgggttg ggaggacatt acatcctccc aacccattac ccatttcaga
     2761 ttaatattct aatagaaatc acttcaacaa catcatttta cgaacattaa cctgaccagc
     2821 acattccagt ttataaaaat acataccacc ggctaaatga ctggcatc
//
LOCUS       DAOSMP010000124         2819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_35629_length_2819_cov_12.909913, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000124 DAOSMP010000000
VERSION     DAOSMP010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35629_length_2819_cov_12.909913"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="PKX09_10190"
     CDS             complement(<1..1180)
                     /locus_tag="PKX09_10190"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOU18231.1"
                     /translation="MNSDKEMLLKELEFALKNEFLDVWYPAALDTVYGGFLSDFTFDW
                     RPEGPQNKMIVTQSRQVWTASQAAMFYQDDQYRKIAEHGFNFLRDKMWDKTYGGFYQL
                     RDRQGNLIPYGDGLHKVAYGNAFAIYALATYYEAFGDTSALDLARQTFAWLEKYNHDP
                     QSKGYVDATNLDGSWIMWHPTGKTNRDITETVLKDYNSSIHLLEAFTQLYKVWPDSLV
                     RERLLEMLTLVRDTFVNKKGYLNMYFTRDWQPLSVYDFSAEPQEADYFFDHVSFGHDV
                     ETAYLLLEAEHILGKKSYSVTLPVARALVDHALENGWDQDNGGLYYQGYYFDGSDTIT
                     IFDDTKEWWVQAEALNAFLLMAKLFPQDQKYYDAFRKQWEYIQRYLIDHQHGGWYSKG
                     L"
     gene            complement(1306..2328)
                     /locus_tag="PKX09_10195"
     CDS             complement(1306..2328)
                     /locus_tag="PKX09_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HOU18232.1"
                     /translation="MMRIPYIFGITVLLIFMVSCQSGSKAVDAFEQNEKLGRGVNILG
                     YDPIWDSFEKARFKEHHFKLIREAGFNTVRINLHPFRHMDPTTFELREHWWNVLDWAV
                     ENALANGLMIILDMHEYYAMADDPVARKDMWLTFWKQVAERYQDSSDDVLFELLNEPN
                     GKLNSELWNDFLLEGLAVVRNSNPHRTVIIGPAQYNQINQLDSLILPEKDRNIIVTIH
                     YYSPMDFTHQGASWAGRTDSLGVQWLGTAEEKQAILDDFAKAQNWSKKHNRPLFLGEF
                     GVYDKADMESRTKYLSFVTRTVENLGWSWAYWQFDSDFILYDIDNEHWIEPVLKALIP
                     KTTDVN"
     gene            complement(2365..>2819)
                     /locus_tag="PKX09_10200"
     CDS             complement(2365..>2819)
                     /locus_tag="PKX09_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18233.1"
                     /translation="NDILIPQIRYATNDSWELITALDSGNGYPIFHQASYANGKLYVL
                     TIPDNFSELYELPREVLTIIKKVMMKNFFVYTDSPSSVSLMVYDNNTFIIYSFKDHNT
                     DVNIVLDNKFSKVTDLLANRELTGYKENNKLVIKTAIGPHSYRVFRAE"
BASE COUNT          829 a          642 c          543 g          805 t
ORIGIN      
        1 ccagaccttt tgaataccag ccgccgtgct ggtgatcaat aaggtagcgt tggatatatt
       61 cccactgttt ccggaaggca tcgtagtatt tctgatcttg cggaaaaagt ttagccatca
      121 ataaaaatgc attcaaggct tcggcttgaa cccaccattc cttagtatca tcaaaaatcg
      181 taatcgtatc cgaaccgtcg aagtagtagc cctgataata caatccgcca ttgtcttgat
      241 cccagccgtt ctcgagagca tggtcaacca gcgctcgggc aactggcaaa gttaccgaat
      301 aggatttttt accgagaata tgttccgctt ccaaaagcaa ataggcagtc tcgacgtcat
      361 gaccgaaaga aacatgatca aaaaaatagt cggcttcctg cggctcggca gaaaagtcat
      421 acaccgatag tggttgccag tctctcgtaa aatacatatt cagataacct tttttattga
      481 caaaagtgtc cctgaccaga gtcagcattt ccagtaggcg ttcgcgcacg agactatccg
      541 gccatacttt gtagagctga gtaaatgcct ccagcaggtg aattgaagaa ttatagtcct
      601 tcaaaaccgt ctcggttata tcgcgattgg ttttaccagt cggatgccac attatccagg
      661 aaccgtccag attggtggca tcaacgtagc ctttggactg gggatcatga ttatattttt
      721 cgagccaggc aaaagtttgg cgcgccagat caagagcaga agtatcgcca aaagcttcat
      781 aataagtagc cagcgcatag attgcgaaag cattgccata agccacttta tgaagaccat
      841 ctccataggg aattaaattc ccctgacgat cccgcaattg ataaaagccg ccataggtct
      901 tgtcccacat tttatctctt agaaaattaa atccatgttc ggcaattttg cgatattgat
      961 catcttgata aaacattgcc gcctgcgaag ccgtccagac ttgccgactc tgagttacaa
     1021 tcatcttatt ctgcggaccc tcaggccgcc agtcaaacgt aaagtcactc agaaaaccac
     1081 cataaactgt atcgagagcc gctggatacc agacatctaa aaattcattt tttagagcaa
     1141 attcaagttc ttttaataac atttctttat ctgaattcat ttgtgtagca catcctatca
     1201 agattattat aattatgcag taaattatta agtgacgata tttattcaag ttatcatcca
     1261 cccttacttt ataattctcc aaatgattat tgccattcca atacctcaat taacatcggt
     1321 agttttgggt atcaaggctt tcaaaaccgg ctcaatccaa tgttcattat caatatcata
     1381 aaggataaaa tcactgtcaa actgccaata tgcccaactc catcctaaat tttccaccgt
     1441 cctggtcaca aacgaaagat atttagttcg agactccata tcggctttat catagacacc
     1501 aaactcgccg agaaacaagg ggcggttgtg tttttttgac cagttttgag ctttggcgaa
     1561 atcgtccaga atggcttgct tctcctcggc tgtgcccaac cattggacac ccagcgagtc
     1621 agttcgacca gcccaggacg caccttgatg agtaaagtcc atcggcgaat aataatgaat
     1681 agttacgatg atattacgat ccttttccgg caatattaaa ctatctaact gattgatttg
     1741 gttgtattgt gctggaccaa tgatgaccgt ccgatgggga ttggaattgc gaaccaccgc
     1801 taaaccttcc aataggaaat cattccacaa ttcactgtta agtttgccat tgggttcatt
     1861 taacaactca aaaagcacat catccgaact gtcttggtaa cgttcggcaa cctgcttcca
     1921 gaatgtcaac cacatatcct tacgagcgac tggatcgtca gccatagcat aatattcgtg
     1981 catatcgaga ataatcatta agccattagc cagcgcattt tccaccgccc aatccagaac
     2041 attccaccaa tgttctcgta attcaaaggt cgtcggatcc atatgccgaa aaggatgcaa
     2101 attaattcgg acggtgttga agcccgcctc ccgaatgagt ttaaaatggt gctccttgaa
     2161 acgcgccttc tcgaacgaat cccagatcgg atcgtagcct aagatattta ctcctctgcc
     2221 aagcttttca ttttgttcaa aggcatccac cgcctttgat ccggattggc aggaaaccat
     2281 aaaaattagt aaaacggtaa tgccaaaaat gtacggtatt ctcatcataa cattctccta
     2341 ttgattaatt tttattgata atcattattc agctctgaac acccgataag aatgcggccc
     2401 gattgcagtc ttgatcacta atttgttgtt ttctttatag ccggttaatt ctcgattggc
     2461 aagaagatca gtgactttac tgaatttatt atccaggaca atatttacat ccgtattatg
     2521 gtctttgaag gaataaatga taaaggtatt gttgtcatag accatcaggc tcacactgct
     2581 gggactatcg gtgtaaacga agaaattttt catcattacc tttttgatga ttgtcaaaac
     2641 ttcgcgaggt aattcgtaca attcgctgaa gttatccgga atagtcagca cataaagttt
     2701 gccgttagcg taactggctt gatggaagat tggatagcca ttgccgctgt cgagcgcggt
     2761 aatcagttcc caggaatcat tggtagcata gcgaatttga ggaatcagaa tatcatttt
//
LOCUS       DAOSMP010000125         2819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_35630_length_2819_cov_11.044501, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000125 DAOSMP010000000
VERSION     DAOSMP010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35630_length_2819_cov_11.044501"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PKX09_10205"
     CDS             <1..536
                     /locus_tag="PKX09_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOU18234.1"
                     /translation="ITSTQKCIGLPPGMAICSVSQRALEHARTVPFRGAYLDLVELYD
                     YLIKKDFQYPSTPSLSHMFALDLQLDYILNVEGLEKRYARHREMAEITRAWAKENFAV
                     FPQPGYESVTLTTITNTRGISIADLNKRLGEAGYQISNGYGELKEKTFRIAHMAERTV
                     DELRTLLNLIDTLIAKM"
     gene            560..1459
                     /locus_tag="PKX09_10210"
     CDS             560..1459
                     /locus_tag="PKX09_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOU18235.1"
                     /translation="MKILIADGLDQKAQNILKEAGLELVVKHYEADDLIKVIQEFDGI
                     IVRSATKVPQVVIDAGKNLKFIARAGTGIDNIDHQYARAKGITVLNAPGANSHSVAEL
                     VFAHLLALARFITPATISLREGRWEKKTFGNGIELAGKTIGIIGFGRIGKIVAHLAIA
                     WGMHVLVYDVATINTEPTVKVVDFETLLKESDFITLHTPKIDQPIINEKEIAMMKHGV
                     YIINCARGNAIDEKALLAGLNSGQVGGAGLDVFAAEPPQNMELLHHARVSCTPHIGAS
                     TLEAQERVGAEIAHRIVEYIGTL"
     gene            1468..2718
                     /locus_tag="PKX09_10215"
     CDS             1468..2718
                     /locus_tag="PKX09_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOU18236.1"
                     /translation="MVKIKPFRGIRPVKELADKIAAPPYDIINFEEARSLAADNPYSF
                     LHVSRAEIDLPPETDPYDEKVYQKARENLEAMLAKGWFFQDATEMLYIYRQIMDKHSQ
                     YGLITTANVNDYLKGKIKIHELTRQDKEMDRTKHLEYTNVNTGPVFLTYPAKPVIDTI
                     IHQIVTESQPEYDFTAVDGIQHTFWLVNDRETILKLVNHFSQLPHCYIADGHHRAKSA
                     VSVAKMRRERNPHHTGNEEYNWFLAAFFPHNQLRTLEYNRTVKDLNNLTEEEFLTKVG
                     EKFIIKPAECAICAKPSAPNHFAMYLNKHWYTLIAKPGTFDATDPVASLDVAILQNNL
                     LKPLLGIDDPRTDKRLDFVGGIRGLEELEKRVDSGEMAVAFALYPTSIEQLMTIADAG
                     KIMPPKSTWFEPKLRSGLVLHSLD"
BASE COUNT          807 a          674 c          611 g          727 t
ORIGIN      
        1 gcattacctc gacgcagaaa tgtattggtc tgccgcccgg gatggcaatc tgttcagtca
       61 gtcagcgagc gctggaacac gcgcgtaccg taccgtttcg cggggcctat ctcgatcttg
      121 tcgagctcta tgattatttg atcaaaaaag attttcaata tccgtctaca ccttctctat
      181 cgcacatgtt cgctctggat ttacagttgg attatattct gaatgtcgag ggtctggaaa
      241 agcgttatgc ccgtcatcgg gaaatggctg aaattacgcg cgcctgggct aaggaaaact
      301 ttgccgtctt cccgcaacct ggctatgaat cagtaacctt gacgactatt actaataccc
      361 gtggtatttc aatagccgat ttgaataaac ggcttggcga agctggttac cagatttcca
      421 atggctacgg cgaactaaaa gaaaagacct tccgcatcgc ccatatggct gaacgcaccg
      481 ttgatgaatt aaggacttta ttgaacctca ttgacacgtt aatcgctaag atgtaaaatt
      541 cttggccaga aaggaaagta tgaaaatatt aattgccgac ggtctggatc aaaaagccca
      601 aaatattcta aaagaagccg gactggaact ggttgtcaaa cattacgaag ccgacgatct
      661 gatcaaagtt attcaagaat ttgatggtat aattgtgcgc tcagctacca aagtacccca
      721 agttgtgatt gacgctggta aaaaccttaa attcattgcc cgggccggca caggtataga
      781 caatattgat catcaatatg ctcgagctaa aggaatcacc gttctgaatg cacccggtgc
      841 taactcgcat tcagtagcgg aattagtctt tgcccacctg cttgctctgg cacgtttcat
      901 cactcccgcc acgatttcct tacgcgaagg tcgctgggaa aagaaaactt tcggtaatgg
      961 cattgaatta gccggtaaga caatcgggat aattggcttc ggacgcatcg gtaaaatcgt
     1021 agctcactta gcaattgcct ggggaatgca cgttcttgta tatgacgtcg caacaatcaa
     1081 taccgaacct accgtaaagg tagtcgattt tgaaacatta ctaaaggaat cggattttat
     1141 tactctccat actcccaaaa tcgatcaacc gatcataaac gaaaaagaga tcgcgatgat
     1201 gaagcacggc gtctatatta ttaattgtgc ccgcggcaat gctattgatg aaaaggcact
     1261 gttagcaggt ttgaattctg gtcaagtcgg cggcgccggt ctggacgttt ttgccgcaga
     1321 accaccgcaa aacatggaat tacttcacca tgcgcgtgtc tcctgcaccc cccatattgg
     1381 cgcgagcact ctagaagccc aggaacgcgt cggcgccgaa atcgctcatc gaatcgtgga
     1441 gtacatcgga accctgtagg aatttatatg gtaaaaatca aaccattccg tggcatccgg
     1501 ccggttaaag aattagcgga caaaatcgcc gctccgcctt atgatatcat aaattttgaa
     1561 gaagcccgaa gtcttgccgc cgacaatcca tattctttcc tgcatgtcag cagagcggag
     1621 atagatttac caccggaaac cgatccctat gatgaaaaag tctatcagaa agctcgcgaa
     1681 aatttagagg caatgcttgc caaaggctgg ttctttcagg atgcgaccga aatgctatat
     1741 atctatcgcc aaataatgga taaacatagc cagtatggat tgattaccac cgctaacgtc
     1801 aatgactatc ttaagggaaa aatcaaaatc cacgaactga ctcgccagga taaggaaatg
     1861 gatcggacca agcatctcga atatactaat gtcaataccg gtccggtatt tttgacttat
     1921 ccggctaaac cggtcattga caccattatc caccaaattg taactgaaag ccagccggaa
     1981 tacgatttta ctgctgtgga tggaatccag catacattct ggctggtcaa cgaccgcgaa
     2041 accatcctca agttagtcaa tcatttcagc cagctcccgc attgctatat tgctgacggt
     2101 catcatcgcg ccaaatcagc cgtttcagtt gcgaagatgc ggcgcgaacg caatccgcac
     2161 cataccggca atgaggaata taactggttt ttagctgcct ttttcccaca taatcagctt
     2221 cgtactctcg aatataatcg cactgtaaag gatttaaata atttgactga agaggaattt
     2281 ctcacgaaag tcggagagaa attcattatc aaaccagccg aatgtgccat ctgcgccaag
     2341 ccttccgcac ccaaccactt cgctatgtat ctaaacaagc attggtacac tcttattgca
     2401 aaaccaggaa ctttcgatgc cactgatccg gttgccagct tagacgtggc gattctgcaa
     2461 aataatctgc tcaagccgct gctcggaatt gacgatccac gcaccgataa acgccttgat
     2521 tttgtcggtg gaatccgcgg cttggaagaa ctcgaaaaac gcgttgattc tggtgaaatg
     2581 gcggtggcat ttgcccttta tccgacttcg atcgaacaat tgatgacgat tgccgatgct
     2641 gggaaaatta tgccgcctaa atccacttgg ttcgaaccca aattaagaag cgggctagtg
     2701 ctccattcgc tggattaaaa tttttattcc tattttgtcc gttctattac tcgccgatta
     2761 aagagcaaac atgccatcat ttgcatttcg taatcagtgc tgtaaattcg accgctatt
//
LOCUS       DAOSMP010000126         2788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_36147_length_2788_cov_9.697768, whole genome shotgun sequence.
ACCESSION   DAOSMP010000126 DAOSMP010000000
VERSION     DAOSMP010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36147_length_2788_cov_9.697768"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PKX09_10220"
     CDS             <1..454
                     /locus_tag="PKX09_10220"
                     /inference="COORDINATES: protein motif:HMM:NF028498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HOU18237.1"
                     /translation="MHSCMEAGFSAYVWWYIVRYYGPISDGEKESSGGGRKGEVTKKG
                     YVMSQYSRFIRPGYHRIKCNSMPQKSVLATAYKDSASSKMVLVVLNISSSSTLQQTFT
                     IPNGNVASFTPFTTTSTKNCQQADKIPVINNRITVTLEPSSITTFVSD"
     gene            567..>2788
                     /locus_tag="PKX09_10225"
     CDS             567..>2788
                     /locus_tag="PKX09_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18238.1"
                     /translation="MRKWISLTLLLMSFLWVNVLAQEMPLAYEVEHTGADCPIPYLPS
                     FEELPIVVSLPDPFEWSDGRGRMANYSDWRYRRAEIKAEIEHYEIGEKPVRPDNITAS
                     YSNDTLRVNVTVNGKTLKLISRIFLPSGEGPFPAVIGMNSPSGSIPSNILLERNIALI
                     QYSHDQVTKYGAQSNSDPYYQLYPHLNADNTGQYSAWAWGVSRLIDGLELVQDVLPID
                     LRHLAVTGCSYAGKMALFSGAFDERIALTISIESGGGGYTTWRFSETLAGVETLGATD
                     HNWFKESMFQFAFSNVSRLPHDHHELMAMVAPRALLVTGNPDYVWLADESGYVGSVAA
                     KKVWEALGIPDRFGYSIVAGHSHCAIPNSQLPDVLAFIDKFLLGQDDVNTNVATSPYT
                     TNLEPWITWTVPTLGNGISFFGKTTLIYPANLQTGVDTTSVTFKWNKLEEAETYFFQL
                     STDPTFRTTFVSEATTDTTLTVTDLAGGQRYYWRVQAKNAEDSLGPWSDYWNFISYIP
                     LPSAPQLVSANLTDPGRADYMTFKWRKDKYAAQYRIQVSRLATFSSLAVPAATTSDTV
                     ITLSGFYEGQKLYWRVQAENIAGSSPWSEPSIFTIILPPSDLIIQSFEQNKVTLKWKD
                     NSKVEDGYVIERKQGEASSFSVLDSLGKNVVQYVDTTIEAGQIYIYRVQAYKGSAGSE
                     YSNEASLNLSGIKEEAIPREYSLSQNYPNPFNPTTAISYHLPKAGYLTIKVYDVMGRE
                     V"
BASE COUNT          789 a          578 c          634 g          787 t
ORIGIN      
        1 aatgcatagt tgtatggagg ctggattcag cgcttatgtc tggtggtata ttgttcgata
       61 ttatggaccc attagcgatg gtgaaaaaga aagtagcgga ggcggtcgca aaggtgaggt
      121 taccaaaaaa ggctatgtga tgtcgcaata ttcgcgcttt attcgtccgg gatatcatcg
      181 gatcaaatgt aattctatgc ctcagaaatc ggtattggcg acggcttata aagatagtgc
      241 ctcgtccaaa atggtgttag tggtgctgaa tatcagttcg tcttcgactc ttcagcaaac
      301 tttcacgatt ccgaacggaa acgtggcgtc atttacgcca tttacgacta ccagtacgaa
      361 gaactgccaa caagccgata aaattccggt aattaacaat aggattacgg ttacgctgga
      421 accttcaagt ataacgacat ttgtatcgga ttaatgggtt atttgtaaat ttaatctgta
      481 aatgtaattt tacaaaaatg agagtgataa ttgatatttc gagtcgttta attaattaat
      541 tttttactta gaaaggagca tcaaaagtga gaaaatggat ctcattaacg ttgttattaa
      601 tgtcatttct ttgggttaat gttttagcac aggaaatgcc gttggcgtat gaggttgaac
      661 acactggggc ggactgccca ataccctatt tgccttcgtt cgaagaattg ccgattgttg
      721 tttctctacc tgatccattt gagtggtccg atgggagagg ccgaatggcc aattattccg
      781 attggcgata tcgaagggca gaaatcaaag ctgagattga acattatgaa attggtgaaa
      841 aaccagttcg tcccgataat atcacagcta gctattcaaa cgacaccctc agggttaatg
      901 taactgtgaa cggaaaaaca cttaaactta tctccagaat cttcttacca tctggtgaag
      961 gtccttttcc ggctgtaatt ggtatgaata gtcccagcgg tagtatccca tctaatatat
     1021 tacttgagcg caacattgcg ctaatacaat atagccatga tcaagtcact aaatatggtg
     1081 cacaaagtaa ttccgatcct tattatcagc tctatcctca tctcaatgcc gataataccg
     1141 ggcaatacag cgcctgggct tggggagtta gccgattaat agacggcttg gaattggttc
     1201 aggatgtact tcccatagac ctcagacacc tagcggtaac cggttgttct tatgccggta
     1261 aaatggcgct tttctcaggg gcatttgatg agcggattgc gcttaccatc tctatcgaat
     1321 ccggcggagg tggttacact acctggcgtt tttcagagac tttagcagga gtagaaacac
     1381 tgggcgctac ggatcataat tggttcaaag aaagtatgtt ccaattcgct tttagcaatg
     1441 tatcgcgttt acctcatgac caccatgagt taatggctat ggtggcacca cgcgccttat
     1501 tagtgactgg aaaccctgat tatgtgtggc tggcagacga atccggctat gtcggaagtg
     1561 tcgccgcaaa aaaagtatgg gaagccttag gaattcctga tcggtttggt tattccattg
     1621 ttgctggtca ttcccactgt gcgattccta atagccagct accggatgta ttagcattta
     1681 tagataaatt tctcctcgga caggatgatg tcaatactaa tgtagctaca agtccctaca
     1741 ccactaacct tgaaccctgg attacctgga cggtaccgac cctcggtaac gggatttcct
     1801 tctttggaaa gacgactttg atttatcccg ccaatttgca aacgggtgtg gatacgacga
     1861 gtgttacctt taaatggaac aagctggaag aagctgaaac ctattttttc caattatcca
     1921 ctgacccaac cttcagaact acttttgtaa gcgaagcgac gacggacaca actctaacgg
     1981 ttactgattt ggcaggcggt caaagatact attggcgggt tcaggctaaa aatgctgagg
     2041 attctttggg accctggagt gattattgga atttcatctc atacattccc ttaccttcag
     2101 cgccccaatt agtctccgct aacttaaccg acccaggtcg agcggactat atgacattca
     2161 agtggaggaa agataaatat gcggctcaat accgcattca ggtatcaagg ttagctacct
     2221 tcagttccct ggcggttcca gccgcaacca cctctgacac agtaattaca ttatcaggct
     2281 tctatgaagg ccaaaaactt tactggcggg tgcaggcaga gaatatagcg ggttctagtc
     2341 cctggagcga accttctatt tttacgatta ttttgccgcc gtcggatctt attatacaga
     2401 gttttgaaca aaataaagtt acccttaaat ggaaggataa ctcgaaagtt gaagacggct
     2461 atgtaatcga acggaaacaa ggtgaggcaa gttctttcag cgtccttgat tcactcggga
     2521 aaaatgtggt gcaatatgtt gatacaacta tcgaagcggg ccaaatctat atttatagag
     2581 tccaagccta taaaggttcg gcgggatcgg aatacagcaa tgaggcatca ttaaatttat
     2641 ccggtattaa agaagaggct attccgagag agtattcgct cagccagaat tatcccaacc
     2701 ctttcaatcc gactactgcc attagttacc acttaccgaa agccggttac ctgaccatca
     2761 aggtttatga tgtgatgggg cgagaggt
//
LOCUS       DAOSMP010000127         2777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_36329_length_2777_cov_21.748714, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000127 DAOSMP010000000
VERSION     DAOSMP010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36329_length_2777_cov_21.748714"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /locus_tag="PKX09_10230"
     CDS             <1..1614
                     /locus_tag="PKX09_10230"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU18239.1"
                     /translation="GSDVDFFQFILPFPFIIGLFGLFVHFNEDKHKAVAVLALFLFTG
                     LMIVLYLNQDDPQPRERDYSYVGSFFAFSIWIGIGAAALLERISQLKNKKLSRPLTYI
                     TAGLLLLALPINVLVANYHEHDRSGNYVASDYAYNILQSCGPNGILFTNGDNDTFPLW
                     YMQEVEHLRRDVKVVNLSLLNTSWYIKQLRNIEPKINIGNISDATIDNIGPIPWKKQK
                     VRISPPPESLLTSDKYPEFRQFGYNPKEFPPMEWEVEPTLKYGNQGYLRVQDIMVLQI
                     LEANRWERPLYFASTVSPDNKLGMEDFMSMEGLTFRIYPQKVKRIDADKMRHNLFNVF
                     RYRNLNNPKVYYDDNIKRLMFNYQTAFIQLAIEELYSGNKERMLATLDTMKYLIPEEI
                     FPIEYDDIYLQIGLLYYDGGRRGEPGQPSELEKRLETLLNKPNVNYRNKLKYASIYAQ
                     VLGDYPKAIQILEELNAQKPNDPEISGYLVKVYEEAQQLDKAVRVLDNWLALYPNDSG
                     AAEMRNLYLSKMTPAQKAQYDSLTKVYHR"
     gene            1626..2666
                     /locus_tag="PKX09_10235"
     CDS             1626..2666
                     /locus_tag="PKX09_10235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018034440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU18240.1"
                     /translation="MSSTPEIAVIIPHYNGVAILRDCLNSLFSSTGLPLDVILVDNAS
                     TDDSVKMVRSEFPAVRILPQSQNCGFAGGCNAGIRATEAPFVLILNNDTIHQAGWLEL
                     LLEKIKSQPELVAVQPKILSWQNREQFDYSGAAGGELDIFGFPFARGRIFETIEMDEG
                     QYDYLPNRIFWASGTAFLARRELLIEAGLFDETFFAHMEEIDLQWRLQLMGYSIGVEP
                     RAVIWHRSGSTLGAEAPLKKYLNHRNSLLMFLANYKPLVTAYLLPIRILLDWMALTFS
                     LLKLDLGRAGAILKAHGWIISHPAVTFKRRRQVKALRRVTDFQIMEKLYRGSVALSYY
                     LLKKKSYLDLVN"
BASE COUNT          757 a          617 c          603 g          800 t
ORIGIN      
        1 ggaagcgatg tagatttctt tcaatttatc ttgccatttc cgtttattat cgggcttttt
       61 ggtctctttg tccatttcaa tgaggataag cacaaagccg tggcagtatt ggcgcttttc
      121 ctatttacgg gactgatgat agttttatat cttaatcagg atgatcccca gccgcgggag
      181 cgagactatt cttacgtcgg gtcgttcttc gcattttcga tctggattgg aattggagcc
      241 gcggctttgc ttgagcgaat ttcccaactg aagaacaaaa aattaagtcg cccacttacc
      301 tatataacag ccggtttatt attattagcc ttgccgatca atgttttagt agccaattat
      361 cacgaacatg atcgaagcgg taattacgtt gcctcggatt acgcttacaa tatcctgcaa
      421 tcctgtgggc ctaatggtat tctttttact aacggtgaca acgatacttt ccctctctgg
      481 tatatgcagg aggtcgaaca tctacgtcgc gacgtcaaag tcgtcaatct tagcctgttg
      541 aacacctctt ggtacataaa acaattacgc aacatcgaac caaaaatcaa tatcggaaat
      601 atttccgatg caaccattga taatatcggt cctattcctt ggaaaaaaca gaaagtgaga
      661 atcagcccgc caccggaaag tttacttacc tcagataaat atcctgaatt tcgccagttc
      721 ggctacaatc ctaaggaatt tccaccgatg gagtgggaag tcgaaccgac tctgaaatat
      781 ggtaatcagg gttatctgcg cgtacaagat attatggttt tacagattct ggaagctaat
      841 cgctgggaac gaccgctcta ctttgcctct acggtttcac cagataataa gctcggtatg
      901 gaagatttta tgtcaatgga gggattgaca ttccgcattt atccccaaaa agtaaaacgg
      961 atcgacgccg ataaaatgcg ccataaccta tttaatgttt ttcgctaccg caatttgaat
     1021 aatcccaagg tttattacga tgataacatc aagcgcttaa tgttcaacta ccagacggct
     1081 tttatccaac ttgctatcga agaactttat agcggcaata aagaacgaat gttggcgacc
     1141 ctcgacacta tgaaatatct aataccagag gaaattttcc ccattgaata cgatgacatt
     1201 tatctccaga ttgggttgct ttattatgat ggcggccgcc gtggtgaacc gggacaaccc
     1261 agcgaactcg aaaaacgcct ggaaacccta ttgaacaaac ccaatgtgaa ttaccgcaat
     1321 aaattgaaat atgctagcat ttatgcccag gtgctgggag attatcctaa ggctattcaa
     1381 atcctggaag aattgaatgc tcagaaacca aacgatccgg aaatctccgg ctatttggtg
     1441 aaagtctatg aagaggctca acaattagat aaggcggtga gagtactgga taactggctg
     1501 gcactttatc ctaacgatag cggtgccgcc gaaatgcgca atctttatct gagtaagatg
     1561 actccggctc aaaaggcgca atacgattcg ctgaccaaag tctatcaccg ctaaaccaat
     1621 ccggtatgtc gtccactccg gaaatcgcgg tcattattcc tcactataac ggtgtggcga
     1681 ttttgcgaga ttgtctgaac tcgttgtttt cctcaaccgg cttacctctc gatgtcatac
     1741 tggttgataa cgcctccacc gacgacagcg ttaaaatggt tagaagtgaa ttcccagccg
     1801 tccgtatcct tccacaatct caaaattgcg gttttgcggg cggctgtaat gccggtattc
     1861 gcgctacgga agcgcctttc gttctaatcc taaataacga taccattcat caagccgggt
     1921 ggttggaatt attactggaa aaaatcaaat cacagcccga attagtcgcc gttcagccta
     1981 aaattctttc ctggcaaaac cgtgaacaat ttgattactc cggagcggcg ggaggcgagc
     2041 tggatatttt cggctttccg tttgcccgcg gtcgaatttt cgagactatt gaaatggacg
     2101 aagggcaata tgattattta cctaatcgaa ttttctgggc ttccgggacg gcttttttag
     2161 ccagacggga attattaatc gaagcgggac tttttgatga aactttcttc gcgcatatgg
     2221 aagaaattga tctccagtgg cgactgcaac ttatgggcta ctctattggc gtcgagcctc
     2281 gcgctgtcat ctggcatcgt agtggttcta ctctgggagc ggaagcgccg ctaaaaaagt
     2341 atctaaacca tcgtaacagt ctgttgatgt tcctggcgaa ttataagccg ttggtgacgg
     2401 cctatctttt accgattcgt attctgctgg attggatggc gctgacattt tccctattga
     2461 aattagattt gggtcgcgcc ggtgccattc tgaaagctca cggctggatt atcagccacc
     2521 cggcggtaac ttttaaacgc cgccgccagg taaaggctct ccgtcgggtt accgatttcc
     2581 agataatgga aaaattatac cgtggttcag ttgccttgag ctattattta ttgaagaaaa
     2641 agtcttattt ggacttggta aattaaaaat taatccgaat aaataatttt ttcgtaaact
     2701 gccttattat ccggatcaag cgccatcagt ttgtgagctt gttcgatagt tccaaattcc
     2761 ttgatgatcg tcattat
//
LOCUS       DAOSMP010000128         2728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_37218_length_2728_cov_13.864572, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000128 DAOSMP010000000
VERSION     DAOSMP010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37218_length_2728_cov_13.864572"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="PKX09_10240"
     CDS             <1..1298
                     /locus_tag="PKX09_10240"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOU18241.1"
                     /translation="NDRLPADGKAFTGIDVLALDIDSLEVADGDTVRAVADYGKWNRP
                     AAATSNGLATFYLQTDSLPGIATLKFSIGEAHTYLTVSFWDTNTTLIAGLCRNAKNSL
                     PIENVLVTLMPDSVQQLTNADGIYYFPQTKPGYYTLNFLKNGYFSQNLEVAVNDGKSN
                     IDTLKMHPIFNGVLHNFVLVLDPGFGGQESVVKISPNLNSAQANLQLAQILKTLFEQA
                     GAEVYLTRDRDSTLSRDQRIKLSNQFPEGGYYLRLNLDCNHQAGRMFKGCHYVGNEEG
                     KRLLQSMANRLTRDGFCDQIQIAGSAEPELVRTNRRALTLDLDLKPQDSLLISNEASL
                     SRLAWGIFLGFVEELGGENLPKGALTINIRASHKPLADAEIFIGSSLRQLSNQTGAIS
                     YEYLEPGKYYLSISHPIYGKREEIFIIQNSSELLINLSE"
     gene            complement(1411..2445)
                     /locus_tag="PKX09_10245"
     CDS             complement(1411..2445)
                     /locus_tag="PKX09_10245"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOU18242.1"
                     /translation="MKNKYFVIGLMILLIVCSHSVFASAPKLGTAGAPELLIPMGAKN
                     VALSGSNIASIAGTDAMYWNPAGMADLKTGEATFTQMSYFADMKVSYLAAGANAGKIG
                     AFGISLQILNIGDIPVTTVNMPEGTGEILSPDYLTLSATYAKRFTDRILFGTNVKLIS
                     EKVGTMSASAVGFDLGLQYISHIGLAFGVTMKNIGSNIEFDGTAIEFDSSVPFANPNA
                     TSRKTKLDMASHELPASMNLGLSYSYKLAELGKINLCGVYSNYSYSYDEMSAGAEFAF
                     RDMLFARAGYLIPLYPSDWEYDETSEYGMSLGFGINLKMAGTNLMFDYAFRPMATFDA
                     NQYFSITIGF"
     gene            complement(2483..>2728)
                     /locus_tag="PKX09_10250"
     CDS             complement(2483..>2728)
                     /locus_tag="PKX09_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18243.1"
                     /translation="WVRFTFLPEKCTIKIFDLSGHLVRELEKNDATTPFMDWDLKNHY
                     GLPVASSVYIYHVEVPGVGEKVGKLAVFTPNERLDTY"
BASE COUNT          818 a          620 c          548 g          742 t
ORIGIN      
        1 ggaatgaccg tctgcctgcc gatggaaaag cttttactgg aattgatgtc ttagccctgg
       61 atatagacag tctcgaggta gccgatggcg atacggtccg agccgtcgcc gactacggaa
      121 aatggaaccg ccccgccgcc gcaacttcca atggcttggc gactttttat cttcagacag
      181 attctttacc ggggattgcg actttgaaat tttcaattgg tgaagcgcat acatacttga
      241 cagtctcttt ttgggatact aataccaccc taattgccgg tttatgccgg aacgcaaaaa
      301 atagcctacc aatcgaaaat gttttagtca ctcttatgcc ggattcggtt caacaattga
      361 caaacgctga cgggatttac tattttccac agaccaaacc cggatattac actttaaatt
      421 tcttaaaaaa tggctacttc agtcagaatt tagaagtagc agttaatgat ggaaaaagta
      481 acatagatac cctaaaaatg catccgattt tcaatggtgt cctgcataat ttcgttttgg
      541 tgctcgatcc gggctttggg ggacaggaat ccgtagtaaa aataagcccg aacttgaatt
      601 ccgctcaggc taatttgcaa ttagctcaaa tcctgaaaac actgtttgaa caagccggcg
      661 ctgaggttta tctgacccgc gatcgtgatt ccacactaag ccgtgaccag cgcattaaac
      721 tcagcaacca atttccggaa ggcggctatt acctgaggtt gaatctggat tgcaaccacc
      781 aagctggcag aatgttcaag ggctgccatt atgtcggcaa cgaagaggga aaacgccttt
      841 tgcaatcaat ggcaaatcgt ctgacccgcg atggtttctg tgaccaaatt caaatcgccg
      901 gttcagctga accggaattg gtcagaacca atcggcgggc tttgacactc gatctggatt
      961 taaaaccgca ggacagccta ctaatcagca acgaagccag cctttcgcgg ctcgcctggg
     1021 gaatttttct ggggtttgta gaagaattag gtggagaaaa tttaccgaag ggagctttaa
     1081 caataaatat cagagccagt cataaaccac tcgctgatgc tgaaatcttt atcggttcgt
     1141 cactaagaca attaagcaat cagaccggtg caatttccta tgaatacctt gaaccaggta
     1201 aatattactt atcaatcagt catccaattt atggcaaacg cgaggaaatc ttcattatac
     1261 aaaatagcag tgaattgtta atcaatttgt cggaatagat tgggataata tttactctct
     1321 aaaaggattt tcacactttc gaaaggttcc ataataaaaa aacggcagtc atttctgact
     1381 gccgtttttc atagttttta cctgtttttc ttaaaaaccg atagtgatag agaagtattg
     1441 attggcatca aaggtcgcca ttggccgaaa agcataatca aacatcaggt ttgtaccagc
     1501 catcttcagg tttataccga atccgagaga catcccatat tccgaagtct catcatactc
     1561 ccaatcgctt ggataaagtg gaattagata accagcgcga gcaaacaaca tatccctaaa
     1621 cgcgaactca gccccagccg acatttcatc gtaggaataa ctgtagttac tataaactcc
     1681 gcataaatta attttaccta attcagctaa tttgtaagaa taagaaagac cgagattcat
     1741 acttgccgga agttcatgag acgccatatc cagtttggtt ttacgggaag tcgcattagg
     1801 attagcaaat ggaacacttg agtcaaattc tattgcagta ccatcgaatt cgatattact
     1861 gccaatattc ttcatcgtaa ctccgaaagc caatcctata tgagaaatat attggagtcc
     1921 caaatcgaaa ccgactgctg aggcactcat cgtaccaacc ttttcgctga tcagttttac
     1981 attagtaccg aaaaggattc gatcagtgaa acgtttagcg taagttgcac tcaaagtcaa
     2041 ataatcggga cttaatattt ccccagtacc ttcaggcata ttaacagttg tgacgggaat
     2101 gtctccaata tttagaattt gcagactaat cccaaaagcg ccaatctttc ccgcattggc
     2161 tccagccgcc aggtagctga ccttcatatc ggcgaagtaa ctcatttggg taaaagtcgc
     2221 ttcaccggtt tttagatctg ccataccggc gggattccag tacatagcat ccgttccagc
     2281 aatgcttgca atattcgatc cgctcaaagc aacgttcttg gcacccattg gaataagaag
     2341 ctccggggca ccggcagtac ccagtttagg agcactggcg aagacgctgt gagaacagac
     2401 gatgagcaat atcattagcc ctattacgaa atatttgttt ttcatattat ttctccttta
     2461 aagtccatta ttcattcaca gcttaatagg tgtccagtcg ttcgttagga gtaaagaccg
     2521 ctaacttacc gactttttcg ccaacacctg gtacctcgac atgatagata taaacactac
     2581 tcgcaaccgg aagcccataa tggtttttca aatcccaatc cataaatgga gtagttgcat
     2641 cgtttttctc caattcacgt accaaatgtc ccgacagatc gaatatctta attgtacatt
     2701 tctccggaag gaaggtaaaa cgtaccca
//
LOCUS       DAOSMP010000129         2667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_38318_length_2667_cov_18.714395, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000129 DAOSMP010000000
VERSION     DAOSMP010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38318_length_2667_cov_18.714395"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PKX09_10255"
     CDS             complement(<1..328)
                     /locus_tag="PKX09_10255"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOU18244.1"
                     /translation="MKALVTGATGFIGQHLIRRLLAENCEVRALCRSTSIKPDEFAQR
                     VEWQIGDITAPESLASAVSDCDLVFHLAGAIKAQDDATFTRINVNGTVNLLKALKQYG
                     KKEVRFI"
     gene            complement(328..1167)
                     /locus_tag="PKX09_10260"
     CDS             complement(328..1167)
                     /locus_tag="PKX09_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOU18245.1"
                     /translation="MLKPLAIESWPDMFFYRPLAFGLVKTIIPLPITPNMISLSAIII
                     GLSGGYCLAIGTPRSCQIAGLLFMANVILDCADGMLARYKHNGTPLGFIVDGLVDYCN
                     GAAIFIGMTVGFSRTDSQFGFSLWVLIPLSLFSWIAHSMAVDHARREFQRNALEQYPS
                     SAGGRQNFQNYREQLSRSKGHWSDKLLASLYLFYCNVQKPNAKFSRTYPADRYYRYNN
                     CVMRGWLLIDPSTHMSIAILSALLYNPLIFFWYTIVIANIIMLMMYLIQYFVNQKVGV
                     LPV"
     gene            complement(1262..1984)
                     /locus_tag="PKX09_10265"
     CDS             complement(1262..1984)
                     /locus_tag="PKX09_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18246.1"
                     /translation="MDNIFDIIILNGRPASGKSEVIDYLKKTPVEIRRRRFHIGELDE
                     IDDFPMLWTWFEEDAILEKILNKPRLHSDKDGYFFWEYLWHLLIERISMEYSKRLRDK
                     PNFHNNYTALIEFSRGSEHGGYRAAYPHLSDEILKWAAIFYIDVSFEESLRKNRQRFN
                     PAKPDSILEHGLPDAKLTRLYKDCDWEEFRGNDPEFIKIKGLKVPYVVLHNEPDITTA
                     RDENLGRVLEELLNKLWILRQK"
     gene            complement(2003..>2667)
                     /locus_tag="PKX09_10270"
     CDS             complement(2003..>2667)
                     /locus_tag="PKX09_10270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU18247.1"
                     /translation="DGDVNLEEKVKFKEVLTKVFGSKVMWTIAIGSMMIGFVRRGVVD
                     AWWPIYFKEMQGVTGVDFAFQLTAWGIAILGIIGGFAFGISSDRVFGGRRAPVIVIGF
                     CGMIVILTLFFLADVWQWGPIAAAILLACLSFFVNGSHGMIAGAATMDFGGRKAAATA
                     VGLIDGMQYIAGAFVGALVGWVTTNWGWQAWKIWPIPFAILGALVMSSIWHTTPTGKS
                     TH"
BASE COUNT          786 a          641 c          581 g          659 t
ORIGIN      
        1 aaatgaaccg cacctctttc ttgccgtatt gcttcaaggc tttcaaaaga ttgacggtgc
       61 cgttgacgtt aatccgggtg aaagtggcat catcttgggc tttgatagca ccggcaagat
      121 gaaagactaa gtcacaatct gagacggctg aagccagcga ttccggagca gtaatgtcac
      181 ctatttgcca ctcgaccctt tgcgcgaatt catccggctt gatactggtg gagcggcaaa
      241 gagcgcgcac ctcgcaattt tctgctagta aacgtcggat aagatgctga ccaataaatc
      301 cggttgcgcc agtgacgaga gctttcatca gaccggcaag actcctactt tttggtttac
      361 aaaatactga atcagataca tcattaacat aatgatatta gcaattacga ttgtgtacca
      421 gaaaaatatc aacggattat ataataatgc cgacagaatt gcaatggaca tatgggtaga
      481 aggatcgatc aataaccaac cgcgcataac acaattgttg tagcgataat atctgtcagc
      541 cgggtaggtg cggctaaatt tggcattcgg tttctggaca ttgcaataga ataggtaaag
      601 gctagccagc aatttatcag accagtgacc cttcgagcgg cttagctgtt cacgataatt
      661 ctgaaaattt tgccgacctc cggctgagga cggatactgt tcgagagcat tgcgctgaaa
      721 ttcccgccgg gcgtgatcga ctgccatcga atgagcgatc cacgaaaaga gactcaatgg
      781 aataagcacc cagagcgaga atccaaattg tgaatcagta cgactgaaac cgacggtcat
      841 acctatgaaa atcgcggctc cgttgcaata gtcaaccaga ccatcaacta taaagcccag
      901 cggcgttcca ttgtgtttat aacgcgccaa cataccgtcc gcgcaatcca gaatgacatt
      961 ggccataaac agcaaaccgg cgatttggca gctccgcgga gtgccgatcg ctagacagta
     1021 gccgccgctt aaaccgatga taatcgccga aagcgaaatc atattcggcg taatcggcaa
     1081 gggtataatg gtcttcacca aaccgaaagc cagcggtcga tagaaaaaca tatccggcca
     1141 actttcgatc gcgagcggtt tcagcattga acgatattct ctgtacaact tatgccagat
     1201 tttcccaggc gatttggtca taaattcatt ttaccgatat aatccgatat aatccaggat
     1261 tttatttctg tcgtagaatc cagagtttat ttaatagttc ttctaatacc cgaccgagat
     1321 tttcgtcgcg ggcagtcgtg atatctggtt cgttatgtaa aacgacataa ggcactttaa
     1381 gtcccttaat tttaataaat tcagggtcat taccgcggaa ttcttcccaa tcgcaatctt
     1441 tatagagacg ggttaacttc gcatccggca gtccatgttc caaaatgcta tccggcttag
     1501 cgggattaaa gcgctggcga tttttacgca gtgattcttc gaacgatacg tcaatgtaga
     1561 aaatagccgc ccattttaaa atttcatcag ataaatgcgg gtaagccgct cgataaccgc
     1621 cgtgttcact accgcgtgaa aattcaataa gagccgtgta gttattatga aaattgggtt
     1681 tgtcgcgcag acgtttactg tattccattg agatacgctc aatgagtaaa tgccaaagat
     1741 attcccagaa aaaataacca tctttgtcgg aatgcagtcg cggtttattc aatatttttt
     1801 ccagaatggc gtcttcttcg aaccaggtcc agagcatcgg aaaatcatca atttcatcca
     1861 actcgccaat atgaaaacgg cgccggcgta tttccaccgg agtttttttg agataatcaa
     1921 tgacctcgga cttgccggat gctggtcgtc cattaagaat gataatgtcg aaaatattat
     1981 ccataaaaat gctccttgat tattaatggg ttgattttcc agtgggagta gtatgccaga
     2041 tagaagacat taccagtgcg cccagaatgg caaagggaat tggccagatt ttccaggctt
     2101 gccagcccca attggtagta acccagccga ctagcgcgcc aacgaatgct cccgcgatat
     2161 actgcatacc gtcaattaat ccgacggcgg tcgcggcggc tttgcgacca ccaaagtcca
     2221 tcgtagccgc tccggcaatc ataccgtggg aaccgttaac gaaaaatgac aagcaagcta
     2281 acaaaatagc cgcggcaatc ggtccccatt gccatacgtc agctaaaaag aatagggtga
     2341 gaattacaat cataccgcag aaaccaatta cgatcacggg cgcacgccgg ccgccaaaga
     2401 cccgatcgga ggaaataccg aaggcaaaac caccgattat tcccagaata gcaatacccc
     2461 aggcggttaa ttgaaaagcg aaatctactc cggtaacacc ctgcatttct ttgaaataaa
     2521 ttggccacca ggcatctacc acgccgcgcc gtacaaaacc tatcatcatt gaaccaatgg
     2581 caatggtcca cataacctta ctgccgaaca ctttggtaag cacttctttg aacttgactt
     2641 tttcctcaag attgacgtca ccatcgc
//
LOCUS       DAOSMP010000130         2612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_39339_length_2612_cov_16.592882, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000130 DAOSMP010000000
VERSION     DAOSMP010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39339_length_2612_cov_16.592882"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="PKX09_10275"
     CDS             complement(<1..1529)
                     /locus_tag="PKX09_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOU18248.1"
                     /translation="MLSKNKLQFAQAFSYVQMRSRLAVFLLMLMLPLIIFANPPTWQP
                     ITGTQYSMIAMVNIRLYDHVFEGGGNNIAGAFGSGGESDCRGIGSWIPPNPPAHNGFW
                     YFTIVGNKNGEEITFKIYDERTDAVYRCHPTLTFIDNSTVGDPDLFELSVQESAISGK
                     IKLLTTTSPAGDIVNVEISAGDVGIHPDAEGDYHLAISPGIYEVTSSLDGYTTVILKD
                     IVVEQNQVTEKIDMTLIDWAPISGNHYNMVVVAKAKINGKIIGGGNNNQIAAFGSGGD
                     EDCRGIGSWIEPSPSYDGYWYFTIRGNEIGEEIGFKIYLEELNAIETIIQTISFEDNA
                     TIGTPNEPLELRNFAKQEFSLQKDWNWVSFNIHPDNSQIDSVFSSLTSSIFQIKSQNK
                     SATFYRAQNKWMGDLTQIDDGKTYLIKMDQAVEKFSVVGTPINVSTPIELIEGWNWIA
                     YYPQNPMAVNSALQSIMPSAFQVKSQTQSATYFDPPSSWVGDLSLMEPNQGYKLKVNS
                     PS"
     gene            complement(1647..>2612)
                     /locus_tag="PKX09_10280"
     CDS             complement(1647..>2612)
                     /locus_tag="PKX09_10280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU18249.1"
                     /translation="DAFNSFTFQGQLINPVIHPIPLSQNWNWIGYLPQNESPLDTALA
                     SIIGSDTIIVKNQSQSAIFIGNTWIGDLTKMAPGIGYKLKITTDDSVTYLTYPFVSGA
                     LSKPLTQKELASNLAGWKVMTGNKSNMIALVEPEINAEKITDSERYTIGVFDSRGNCR
                     SVGKKEENCWYFTIVGNEDGESLNFKIWDAKKECELVTEQKIQFVSDALVGAPSEPLV
                     VKFSGSEAPDVSLTLPTELTLEQNYPNPFNPVTTISYTIPEPGIVNLKIYDLKGNLVK
                     TLVDKFQDPGNHVVEWNAGDLSSGIYFYKIQYGGQSLIKRCLLIK"
BASE COUNT          744 a          580 c          483 g          805 t
ORIGIN      
        1 gaaggactgt ttacttttaa tttataccct tgatttggct ccatcagact gaggtcgcct
       61 acccaagaac tgggtggatc gaagtacgtc gccgattgag tttggctttt tacttgaaaa
      121 gcactgggca taatactctg caaagccgaa tttactgcca taggattttg tggataatag
      181 gcaatccaat tccatccttc gattagttcg attggagtgg atacatttat aggtgtacct
      241 actaccgaaa atttttcaac tgcctgatcc atcttgataa gataggtttt cccgtcatca
      301 atttgggtca aatcacccat ccatttattc tgcgctcgat aaaaggtcgc ggatttattt
      361 tgactcttta tttgaaaaat gctactggtt aaactggaaa aaactgaatc tatctgagaa
      421 ttatcaggat gaatattaaa tgatacccaa ttccaatctt tttgtagaga aaattcttgc
      481 ttggcgaaat ttctcaattc gagcggttca ttaggagtgc cgatggtcgc attatcctcg
      541 aaagaaatag tctggataat tgtttcgatt gcattgagtt cttccaagta aattttaaaa
      601 ccaatctctt cgcctatttc attcccccga atagtaaaat accaatagcc atcgtaggaa
      661 ggtgaaggct cgatccaact ccctatccct cgacaatctt catcacctcc cgaaccaaaa
      721 gccgctattt gattattatt cccgccaccg ataattttgc cgttgatttt tgctttcgct
      781 acaaccacca tattgtaatg attgccgcta attggcgccc aatcaatcag cgtcatatca
      841 attttttcgg taacctggtt ttgctcaaca acaatatcct ttaatataac cgtggtatag
      901 ccgtccaggc tggaggtaac ttcatatatc ccgggagaaa tagctagatg ataatcgccc
      961 tcagcatcgg gatgaatccc aacgtcgccc gccgatattt caacatttac gatatcacct
     1021 gccggtgatg tagttgtcag taacttgatt ttccctgaga tggcactttc ttgaaccgag
     1081 agctcgaata agtccggatc accaactgtt gaattatcaa taaatgtcaa ggtcggatga
     1141 catctataaa ccgcatcggt tctttcatca tatattttaa aagttatctc ttcgccattt
     1201 ttgttaccga caatcgtaaa ataccaaaaa ccgttatgcg cgggaggatt aggcggtatc
     1261 caggaaccta ttcctctaca gtcagattcg ccgccggaac caaatgcccc ggcaatgtta
     1321 tttccaccgc cttcaaaaac atggtcataa agcctaatat tcaccatcgc aatcatagaa
     1381 tattgcgtgc cagtaatcgg ttgccaggtc ggcggattgg caaaaatgat cagtgggagc
     1441 attagcatta ataggaaaac ggcgagtctg ctcctcatct gaacatagct aaaagcttgc
     1501 gcaaactgca atttattttt acttaacata ttcttgtcct cctaaatccg tgtaaacggt
     1561 atttttagga tttattaaac attcaaggac agaattaatc caataatcac aaatccctac
     1621 ttccttattg taaagattct tagatcctat ttaattaata aacatctctt gattagggac
     1681 tgaccaccgt actggatttt gtagaagtat attcctgaac ttaaatctcc ggcattccat
     1741 tccacaacgt gattgcccgg atcttgaaat ttatcaacga gggtttttac cagattacct
     1801 tttaagtcat atattttcaa attgacaata cccggctcgg gtatcgtgta gctaattgtg
     1861 gtaaccggat taaagggatt cgggtaattt tgctcgagcg ttaattccgt aggcagggtt
     1921 agagaaacgt ctggcgcttc agagccacta aatttgacaa ctaacggttc actcggcgcg
     1981 ccaactaaag catcggaaac gaattggatt ttttgttctg taaccaattc acattctttt
     2041 ttcgcatccc aaatcttaaa attaagcgat tctccgtcct cattgccgac aatcgtaaaa
     2101 taccagcagt tttcctcttt cttcccgaca gaccgacaat taccgcggct atcaaaaacc
     2161 ccaatcgtat atctctcgga atcagttatt ttttcagcgt taatctccgg ctctaccagc
     2221 gcaatcatat tgctcttatt gcccgtcatt actttccaac cggctaaatt agatgcaagt
     2281 tctttttgag tcaacggctt agatagggca ccggagacaa aaggataagt tagataggtt
     2341 acactatcat ccgttgttat ttttaattta taaccaattc cgggcgccat tttggtaagg
     2401 tcaccgatcc atgtattgcc gataaaaata gccgattgac tctggttttt aacgattatg
     2461 gtatcgctac caattatact cgccagcgcg gtatcaagcg gtgattcatt ctgcgggaga
     2521 tatcctatcc agttccaatt ttggcttaga ggaatcggat gaattaccgg attaataagt
     2581 tgtccctgaa acgtaaaaga attaaatgca tc
//
LOCUS       DAOSMP010000131         2583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_39943_length_2583_cov_2.959652, whole genome shotgun sequence.
ACCESSION   DAOSMP010000131 DAOSMP010000000
VERSION     DAOSMP010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39943_length_2583_cov_2.959652"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PKX09_10285"
     CDS             complement(<1..414)
                     /locus_tag="PKX09_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18250.1"
                     /translation="MFKTKIKNHPLFLISAVTVVLMFSNTTFAQDQRWIIVGETQCFF
                     MDYGAETERELNFLTWPTQYGDNQHTLRMNGLWLGARNFFDPVENKEKSVKVIGSGPR
                     LPANHYTMIFPQSIKLIGRHSHPVITVDNQTGTNNL"
     gene            complement(439..>2583)
                     /locus_tag="PKX09_10290"
     CDS             complement(439..>2583)
                     /locus_tag="PKX09_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU18251.1"
                     /translation="PDYDIDAGFGGPVPLVSEKLGNLRFYTSYRRHRDVLLWAQATPD
                     YVDYDWTMQLTSDISETMKLRISSLLGSISTIAENWNYAYYPHWPNEIANGTGGLLMF
                     NMFSDWVYSKTDIGHRSFAIKLTHTLSSKTYYEVSLENFQRDYHTRPIGLRDTTKTTE
                     VVPGYWVDEYPFGYWPYEVVGITPEIKDGIQASLARDYSNASATSFKADLSSQVNFHN
                     LIKTGVEFVYNDLDLDYGFIQMQTKGQAYASRVQMQNFPIRAAFYIQDKLETDGFTMN
                     AGLRLDYSDARVEWWDIFSDPFNPYFISSKYKEDLEFDMVKSKPQWQLSPRLGISHPI
                     TENSKLFFNYGHFKQMPQYETLFRVQRSSTGSLRAIGDPNLTLAKTISYELGYDHLLF
                     NSVLVQLAAFYKDISDQQNETTYESINNDTYILITSNGYEDIRGFELTLRKSQGRWFT
                     GLINYTYQASSRGYFGRREIYQDPAHQQRYDEETSNLYQERPVPTPYARANLSFYTPD
                     DFGPVVLNHHILGGFMLNLLLTWEQGGWTTYNPKNASGVINNVQYLDYYGGTLRAGKT
                     VTSKRFRIQFLVDVNNLFNRMTLWDTGDQKYRESLHLPKSDAYGNIPGDDKLGDYRKP
                     GVEWQPMKYQEIIQGTTPPALNTNTIPIYYEGSTGTYWEIVDNQWIEVSKSKIDQINA
                     DKAYIFNPGASTWWFLNPRTVVFGVRLSFDLD"
BASE COUNT          747 a          544 c          561 g          731 t
ORIGIN      
        1 aagattatta gtgccagttt gattatccac agtaatcact gggtgactat gtcgcccaat
       61 aagtttgata ctctgcggaa aaatcatagt gtaatgattc gccgggagtc ttggaccaga
      121 tccgattact ttcactgatt tttccttgtt ttcgactgga tcgaagaaat tgcgagctcc
      181 cagccacaat ccattcatcc ggagagtgtg ctggttgtca ccatattgag taggccaggt
      241 tagaaaattt aattcgcgtt cggtttctgc gccatagtcc ataaaaaagc actgcgtttc
      301 accgacgata atccagcgct ggtcttgagc aaaagtcgtg ttagaaaaca ttaaaacgac
      361 tgtcaccgca ctaattagaa ataaggggtg attttttatt ttcgttttaa acattggtat
      421 tcctcatttc ttttataatt aatccaaatc aaaagaaagc cggacgccaa aaaccactgt
      481 ccgcggattc agaaaccacc aggttgaggc cccggggtta aaaatatagg ctttatcggc
      541 attgatctgg tcaattttgg atttggaaac ctctatccat tgattatcaa ctatttccca
      601 atatgttcca gtgctaccct cgtaataaat tggaatggta ttcgtattaa gagcaggtgg
      661 agttgttcct tgaatgattt cctggtattt catcggttgc cactcaactc ccggtttacg
      721 gtaatcaccg agtttatcat ctccaggaat attaccatag gcatcactct tgggcagatg
      781 caatgattca cgatacttct ggtcgccggt atcccataat gtcatacgat taaatagatt
      841 gttaacatca accaaaaatt ggatcctaaa tcgtttactg gtaacggtct tgccagcccg
      901 tagagttcca ccataataat ctaaatactg gacgttattg ataacacccg atgcattttt
      961 aggattatag gtagtccagc cgccttgttc ccaagtcaat aaaaggttaa gcataaaacc
     1021 acccaggata tggtggttga gaacaaccgg cccgaaatca tccggagtat agaaactcag
     1081 gttggcacgc gcatagggag ttggcaccgg tcgttcttga tataaattac tggtctcctc
     1141 gtcataacgt tgttgatgag ccggatcctg ataaatttcg cgccgtccaa aatagccgcg
     1201 agacgaagcc tgataagtgt aatttatcag accagtgaac cagcgcccct gacttttacg
     1261 gagtgtcaat tcaaaaccgc gaatatcctc gtaaccgtta ctagtaatta aaatataggt
     1321 atcgttatta atagattcgt aagttgtctc attttgctga tcgctgatat ctttataaaa
     1381 cgcagccagt tgaacgagaa ccgaattgaa aagcaggtgg tcgtaaccaa gttcataaga
     1441 aatagtcttg gctagggtca gattgggatc gccgattgcc cggagactgc cagttgaact
     1501 gcgttgtacc cgaaacaggg tttcgtattg gggcatttgc ttaaaatgcc cgtagttgaa
     1561 aaacaatttg gaattttcgg ttatgggatg tgaaataccc agccgtggac ttaattgcca
     1621 ctgcggtttg gattttacca tatcaaattc gaggtcttct ttatatttgg atgaaatgaa
     1681 atagggatta aatgggtcag aaaaaatatc ccaccactcg acacgcgcgt cgctatagtc
     1741 caaccttaat ccggcattca ttgtaaaacc atcagtttcc agtttatcct ggatataaaa
     1801 ggcggctcgg attgggaaat tttgcatttg cacccggctg gcgtaggctt gacctttagt
     1861 ttgcatctga ataaaaccat aatcgaggtc aaggtcgtta tacacaaatt ccacgcccgt
     1921 tttaatcaaa ttatggaaat taacctggct ggaaagatcg gctttgaaag atgtagcact
     1981 ggcattcgaa taatcacgag ccagtgaagc ctgaatgccg tctttaatct cgggagtaat
     2041 tccgactact tcataaggcc agtaaccgaa agggtactca tcgacccagt atcctggcac
     2101 tacttcggtg gttttggtcg tatcccgtaa tccaattggg cgggtatgat agtcacgctg
     2161 aaaattttcc agagaaacct cataataggt tttagaactt aaggtatgag ttaattttat
     2221 tgcgaaggaa cgatgaccga tatcagtttt agaataaacc cagtcactga acatattaaa
     2281 cattagtaag ccgccggtgc cgttggcaat ttcattcggc cagtgagggt agtaggcata
     2341 attccagttt tcagcaatag tagagatgct ccccagcagg cttgaaatgc gtaatttcat
     2401 cgtttccgaa atatccgaag ttagttgcat agtccagtca taatcaacat aatcgggagt
     2461 agcctgtgcc catagtaaaa catcacggtg acgacgatac gaagtataaa atcggagatt
     2521 gccgagcttc tcggaaacca gtggcaccgg gccaccgaat ccggcatcaa tatcgtaatc
     2581 agg
//
LOCUS       DAOSMP010000132         2520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_41263_length_2520_cov_18.490872, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000132 DAOSMP010000000
VERSION     DAOSMP010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41263_length_2520_cov_18.490872"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PKX09_10295"
     CDS             complement(<1..196)
                     /locus_tag="PKX09_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18252.1"
                     /translation="MEELKILLAESNAETAKRISTIVTNLGYQVIETVSNGTSAVKKA
                     IELKPDLVLISSSLKGKLNGV"
     gene            complement(568..2352)
                     /locus_tag="PKX09_10300"
     CDS             complement(568..2352)
                     /locus_tag="PKX09_10300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOU18253.1"
                     /translation="MKELKILLAESNAETAKSISTIVTNLGYQVIETVSNGPSAVKKA
                     IELKPDLVLISSSLKGKLNGVDTFTQIRQNLDIPIVFIIGQSEKLDLEKLKQVDANGY
                     LFEPFTPEQVRCIIELALSNHEVEQNLVERQALLSTTLKSIGDAVITTDSNGLITFLN
                     PVAEKLTGWSLSEAVGKPLLKVFNIINESTGKKPPNPVKKILEKGLVIELANHTVLIS
                     RNGQKIPIDDSGAPIKDEYGNILGAVLVFRDITEQRKAKKDMEYEKSLLDALMNNIPD
                     SIYFKDRQCRLLRINRGMMKSLNMTQESDYYLKTDIDLFGEEFGRETVKADLHLIETG
                     EPIVGMIESRKLDDGSLNWTSTTKAPIWDANDNIIGLVGVTRTINDIKRAEEALQYEK
                     SLMDALMNNIPDSIYFKDRQCRLLKISRHMMKSLNMTQESDYYLKTDIDLFGEEFGRA
                     TVESDLQLMETGKPIIGLVEGRKLPDGQINWTSTTKIPLRDQDGKIIGLVGISREINE
                     IKQAEEQREKIIAELQEALDKIKTLKGLIPICASCKKIRDDQGYWNNVESYIKSHAEV
                     EFTHGICPDCMKKLYPNYCKDDENKNET"
BASE COUNT          653 a          487 c          495 g          885 t
ORIGIN      
        1 caactccatt tagtttgcct ttaagcgatg agctaatgag caccaggtca ggctttagtt
       61 cgatagcctt tttgacggcg gaagttccat tggatacggt ttcgatcacc tgataaccta
      121 aattagtgac gatggtgctg atccttttag ctgtttcagc attactctcg gctaacaata
      181 tttttagttc ttccattatc cttccctcat taatttggtt gatatttttc tgtattttag
      241 ataattatta gcttgctttt cgaaaatctc cacaatcata ttttacaaaa ataatcttaa
      301 actatcaatg acacaaattt ttaagtaccc tccaatattt ttagggttta tttcctaatt
      361 tattggtaat tcttgtaata taataccaaa caaatggcta cgtatatttt taagcaattg
      421 agttctgctc ttcttattga aatttggggt ttagacacta actaaaatct gagacttgag
      481 taaagactgc tttataaaaa gtcacatagc taaaaaatta ggagaaatcc tggtttataa
      541 gttagcctaa tttagcctaa ttatgtctca agtttcgttt ttattttcat cgtctttaca
      601 gtaattggga tacaattttt tcatacaatc tgggcaaatg ccgtgggtaa attccacttc
      661 ggcatgggat ttaatgtagg attcgacatt attccagtaa ccctggtcgt ctctaatttt
      721 cttacaggag gcgcagatcg ggattaagcc tttcaaggtt ttgattttgt cgagtgcttc
      781 ctgcaactcg gcaatgattt tttcgcgttg ttcttcggcc tgcttgattt cgttgatttc
      841 gcggctgatt ccgacgagtc cgataatttt accatcttga tcacgtagag ggattttggt
      901 agtggaggtc cagttaatct gtccatctgg caattttcta ccttctacca aaccaataat
      961 tggtttgcct gtttccataa gttggaggtc ggattcgact gttgctcggc caaattcttc
     1021 gccaaaaagg tcaatgtcag tcttgagata atagtcactt tcctgagtca tattcaggga
     1081 tttcatcatg tggcgactga ttttgagtaa gcggcattgt cggtctttga agtagatact
     1141 gtccgggatg ttattcatta aggcgtccat caacgacttt tcatactgca atgcttcttc
     1201 agcccgttta atatcattga tcgtccgagt tacgccaacc agcccaatga tattatcatt
     1261 ggcatcccaa ataggagctt tagtagttga agtccaattc aaactgccgt cgtctaattt
     1321 acggctttct atcataccaa caattggttc gccggtttct ataagatgaa gatccgcttt
     1381 aactgtctct cgaccaaatt cttcgccaaa aaggtcaatg tcagtcttga gataatagtc
     1441 actttcctga gtcatattca gggatttcat cataccgcga ttaattctga ggaggcggca
     1501 ttgtcggtct ttgaaataaa tgctgtctgg gatattattc atcagggcat ctaacagcga
     1561 cttttcgtac tccatatctt ttttagcttt gcgctgttcg gttatatcgc gaaaaaccag
     1621 aactgctcct aagatattac catattcgtc tttgatgggt gcgccgctgt catcaatcgg
     1681 tattttctgt ccattgcgcg agattaatac cgtatgatta gctaattcta taactaaacc
     1741 tttttctaag atttttttga ccggattggg tggttttttc ccggtggatt cattgatgat
     1801 attgaatact tttaggagtg gttttccgac ggcttctgac aatgaccagc cggtcaattt
     1861 ctcggcgact ggattgagaa aggtgattaa gccgtttgaa tcggtggtaa tgacggcgtc
     1921 gccgatactt ttgagcgttg tggaaaggag cgcctgtctt tcaactaaat tttgttcaac
     1981 ttcgtgattg gagagagcca gttcgataat gcaacgaacc tgttcggggg taaacggttc
     2041 gaaaagataa ccgttagcat ctacttgttt taatttttcg aggtctaatt tttcagattg
     2101 tccgattata aagacaatcg ggatatcaag attttggcga atctgggtga aagtatcaac
     2161 tccatttagt ttgcctttaa gcgatgagct aatgagcacc aggtcaggct tcagttcgat
     2221 agcctttttg acggcggaag gtccattgga tacagtttcg atcacctgat aacctaaatt
     2281 agtgacgatg gtgctaatac ttttagctgt ttcagcatta ctctcggcta acaatatttt
     2341 tagttctttc attgtccccc tcttggttta gatgatgcat cactgcattt taggtaactt
     2401 tttaatttac ttttcaatta tccccacaat cgtattttac aaagattatt ttaaactacc
     2461 aatgtcacaa attaaagacg gctggtgatt taccaattac gtggattagc gaattaatct
//
LOCUS       DAOSMP010000133         2511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_41454_length_2511_cov_7.682818, whole genome shotgun sequence.
ACCESSION   DAOSMP010000133 DAOSMP010000000
VERSION     DAOSMP010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41454_length_2511_cov_7.682818"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="PKX09_10305"
     CDS             complement(<1..986)
                     /locus_tag="PKX09_10305"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOU18254.1"
                     /translation="MINGIISIIVLSLTTTSLPAQTITRPKIGLALSGGGAMGFGHIG
                     TLKLLDSLSIPVDYIAGTSMGGIIAALYAVGYTGAEIEEIARNVDWADLFNDAPSRQA
                     LPYFQKQEADKYQFELGIRDLRPVDKGGVIAGQKITLYMTRLVQPFLTVSDFDSLIIP
                     YRCVAVDLTTGSEVVIDHGSLPIAMRATMAIPSIFTPVVWGDSLLVDGGLLNNLPVNT
                     VRKMGADIVIASTVRSPFKTRDELRTTLDILSQTSNILRDNKLEAEAKNADLLINCQL
                     EKLGAADFSTAKVCKIIEAGEAAAYAKLDELLNLKKKYNLSRYGPENINDLNS"
     gene            complement(1058..>2511)
                     /locus_tag="PKX09_10310"
     CDS             complement(1058..>2511)
                     /locus_tag="PKX09_10310"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU18255.1"
                     /translation="NTLRQFIADSLKFVYGERYQQELDSLESIYMTTIKTLETECTRL
                     NNLNKILIDSLEKARSARPKSVIETKDLDPTFESRYFQYGKILRLEEPRHKTGFLKMP
                     TVIENIAPFQIQELNEYYDRYFPIARSDSVLDFIIQINIREKDWGNAGCNIIKFLYLF
                     PNSPLGEEIKTVRAGIFRTEKYYQPYSAFLTDLLNKIPQLPLADQRYYHYVELLKGFP
                     DQAIRNNFIPEAYKFLELYPNSKYAAQANLWIAEALLANNQPHSAYLTLQKIMILYPS
                     DELYCRALHTSGVIQQEQFNEYKNAVQTFTDLIKRFPQDTLAENAHYRIAKISDEYLN
                     DWERAVEQYQIYADKYPQSPNAIPALMRKAKIQSTQMNLIEEAVRTYKSIDERYPSTT
                     GAFNALTEAGNLYMTKGRYDQAIEIYMSIFQKYPQSENAVAALEKVYNIYQTKIKDNE
                     KSIEILNLIINNYPDTKASARATKQLRKLEKAR"
BASE COUNT          691 a          521 c          544 g          755 t
ORIGIN      
        1 gaattcaaat cattaatatt ctcaggacca tatcttgaca agttatattt tttcttcaaa
       61 tttaataatt catcgagctt ggcatacgcc gcggcttcgc ctgcctcaat aattttgcat
      121 accttggcag tcgaaaaatc agccgctcct aacttttcta actgacagtt gattagtaaa
      181 tcggcatttt tagcttcggc ttcaagctta ttatcacgca aaatattgga tgtttgggac
      241 aagatatcca aggtggtccg tagttcatcc ctagttttaa aggggctcct aacggttgaa
      301 gcaattacga tgtcagcacc catttttctg acagtattga ctggcagatt attgagcaat
      361 ccgccatcaa ccagaagcga atcgccccac acaaccgggg taaaaatact aggaatcgcc
      421 atcgtcgccc gcatcgcaat cggcagagaa ccatgatcta taaccacttc cgatccagtg
      481 gtcaaatcaa ctgcaacaca ccgatatggg ataatcaaac tatcaaagtc gctgacggtt
      541 aaaaatggct gaactaagcg ggtcatatag agcgtaattt tttggccagc gatcactccc
      601 cctttatcta ccggccgcaa atcgcgaata cctaactcaa attgatactt atcggcttcc
      661 tgcttctgaa aataaggtaa agcctgtcgg gacggtgcat cattgaacaa atccgcccaa
      721 tcaacatttc gagcgatttc ttcgatttct gcaccagtat aaccgacggc gtataaggcg
      781 gcgataattc ctcccatact ggttccggcg atataatcaa ccggaatgct caatgaatcg
      841 agcaatttta gggtgcctat atgtccaaaa cccatcgcgc cgccaccgct cagagccagt
      901 ccgattttgg ggcgagttat cgtttgagcc ggtaaactgg tggtagttag agaaagaaca
      961 ataatggata taattccatt tatcataaat cggtgtctgt taaaattgcg gttagagcaa
     1021 cttgccatcg gaaaattcac acgcagtcta tagggaacta tcgggctttc tcgagcttcc
     1081 tcaattgctt agtcgctctg gcagaagctt tggtatccgg ataattgttt ataattaagt
     1141 ttaatatttc gatgcttttc tcattatctt tgattttcgt ttgataaata ttgtaaactt
     1201 tttcgagagc cgcgacggcg ttttccgatt gcgggtattt ctgaaaaata ctcatataaa
     1261 tttcaatagc ctgatcgtag cgtcccttgg tcatataaag attgcccgcc tccgtcagag
     1321 cattgaatgc gccggtcgtg gaaggatagc gttcatcaat ggatttatag gtccgcacgg
     1381 cttcttcaat taaattcatc tgggtagatt gaatcttggc tttccgcatt aaagccggaa
     1441 tagcgttcgg ggattgggga tatttatcgg cataaatctg atattgttcg acggctcgtt
     1501 cccaatcatt taaatactcg tcgctgattt tagcgatacg ataatgggca ttctcagcca
     1561 gggtatcttg cggaaagcgt ttaattaaat cagtaaaagt ttgaacggcg tttttatatt
     1621 cgttgaactg ttcctgctga ataactccgc tggtatgcag agcgcggcaa tataattcat
     1681 cacttgggta aagtatcatt attttctgta atgtcagata ggcactgtgc ggctggttgt
     1741 tagctaaaag agcttcggct atccacaaat tggcttgcgc cgcgtatttg gaatttggat
     1801 atagctccag aaatttatag gcttccggta tgaagttgtt tcggatagcc tgatcaggaa
     1861 agcccttgag taattccaca taatgataat agcgctggtc tgcgagcggt aattggggaa
     1921 tcttattaag caggtcggtt aaaaaagcgc tataaggttg gtaatatttc tccgtccgaa
     1981 agatgccagc ccgaacggtt ttaatttcct caccgagcgg cgaattgggg aaaagataaa
     2041 gaaacttgat gatattgcat ccggcattac cccaatcttt ttcgcgaata ttgatttgga
     2101 tgataaaatc gagaactgaa tcactccgag caataggaaa atatctatcg taatattcat
     2161 tcaattcttg gatttgaaaa ggtgctatgt tttcgatcac ggtcggcatt tttaaaaaac
     2221 cagttttatg tctgggctct tccagcctga gaatttttcc gtattgaaag tatcgggatt
     2281 caaaagtggg atcgaggtct ttggtttcga tgacagattt cggtctggca gatcgggctt
     2341 tctcaaggct gtcgattaag attttattca aattattcag gcgagtgcat tcagtctcca
     2401 gagttttaat cgtggtcata tagattgatt ccagagagtc aagttcttgc tggtaccttt
     2461 ctccatagac aaacttgagt gaatcagcaa taaactgccg aagcgtgtta a
//
LOCUS       DAOSMP010000134         2468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_42459_length_2468_cov_6.291753, whole genome shotgun sequence.
ACCESSION   DAOSMP010000134 DAOSMP010000000
VERSION     DAOSMP010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42459_length_2468_cov_6.291753"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PKX09_10315"
     CDS             <1..365
                     /locus_tag="PKX09_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18256.1"
                     /translation="RNIDFPDTCMQAVYATACNDVYLVGEGMLYYDDRKINDWPLPGE
                     VPRNLLQSIRGNGPNDLFLAGHLGTVIHYNGNTWRYYEDLFEANTLYAISVKNDCIVA
                     VGLGNQQGIIYHGRREKK"
     gene            375..2450
                     /locus_tag="PKX09_10320"
     CDS             375..2450
                     /locus_tag="PKX09_10320"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOU18257.1"
                     /translation="MKIKSIIFFIVISCASRLFCVTVEYTFRAINLPDGWYDVISFKA
                     EKVSTVFDCNHEPTNLYNGAIVSTPLTYNQDHIYGCFDWFNDDDGHPKLAYGLYKISV
                     QYAGKSAYFYFDLRDCYYSACGIDPPTPFTSYPLDMYFHYDFDDDKFYKASGEIVPIP
                     ISNGSTVGIWLNQVGGDPPGPCTDCFNPKVYLKNQYASNINFGRLVIDETKYINSGEF
                     VNLPINTSHIVRSEQDKYIVDNDSTQQHHWNEDETNISFKRNISINNWTMYEESNFID
                     LIPCILKTNSLTGGICTIPIEFRDPWLVESNYTRPNTYRTYGSPFYPGNGYGVFPNQK
                     KTPENPNAYVYSIKVPSYFDDVDLISVFSHWSVNPSDGVELDVDSPETDVVFKTSGVV
                     VTANFYQYRRSAFQGSIPIDLTLNCNINVVGDVNITEAGNVVVQPSTVFKIAQNKIIR
                     VYGDFTASGSGIFFKPVDPNYTIKNYWKGIYTYSTGKTVLNNVRVEGANYGLYIYYGR
                     GYVYNCTFYNNNYGLRILYPNSSFNIYQCTFTNNNTAAYSSSANVLVANSAFSNNTYG
                     LKTYSSTGNILSNVFSNNSQGIAIYSGSLDFSTSTKNQSYINNTIQNNSQYGIYIGSS
                     AYPTLGEYYVDSKGYTHGGFNLFSRGTGAYDVYSLYASSIDARMNWWASNANRYGNLS
                     FTPKTGEVG"
BASE COUNT          746 a          442 c          496 g          784 t
ORIGIN      
        1 tcaggaatat agattttccg gatacctgta tgcaggcggt ttatgcgaca gcctgtaatg
       61 acgtttatct ggtcggcgaa ggcatgctct attatgatga caggaagatt aacgattggc
      121 cgttaccggg cgaggttccg cgaaatcttc tacagtctat tcgtggtaat ggtcctaatg
      181 acctctttct ggccggacac ctgggtacgg ttattcatta caatgggaat acgtggagat
      241 attacgagga cctttttgag gcaaatactc tttatgcaat ttcagtaaaa aatgattgta
      301 tcgtggctgt gggacttgga aaccaacaag gaattattta tcatggtcga agagaaaaaa
      361 agtaggagga tattatgaag attaagagta ttattttttt catagtgatt tcatgtgctt
      421 ctcgcttgtt ttgtgtaact gtagaatata catttagagc tattaacctt ccggatggat
      481 ggtatgacgt catcagcttt aaagctgaga aagtttcaac tgtcttcgat tgtaaccacg
      541 aacctaccaa tctatacaat ggtgctatag tatctacacc attaacctat aatcaggatc
      601 atatatatgg gtgctttgat tggtttaacg atgatgatgg ccatcccaag ttagcttatg
      661 gattgtataa aattagtgtt caatacgcag gtaaaagtgc ttatttctat tttgatcttc
      721 gggattgtta ctattcagca tgtggaattg atcctccgac accatttacc tcttatcctt
      781 tggatatgta ctttcattat gattttgacg acgacaagtt ttacaaggca agtggggaaa
      841 tagtacctat acctatatcc aatggttcaa cagttggaat ttggttaaat caagttgggg
      901 gcgatccacc gggaccatgt acggactgtt ttaatccaaa agtatatctg aaaaatcaat
      961 atgcatcaaa tattaatttt ggacgtttgg tgatagatga aactaagtat attaattctg
     1021 gcgaatttgt gaatttacca attaatacga gtcatattgt tagatcagaa caagataagt
     1081 atatcgttga taatgattct actcaacaac atcattggaa cgaagatgag actaatatat
     1141 cttttaagag aaatatttct ataaacaatt ggactatgta tgaagaatca aattttattg
     1201 atcttattcc ctgcatactc aagaccaatt ctttaaccgg tggcatttgc acgatcccta
     1261 tcgaatttag agatccctgg ctggtcgaat caaactacac ccgacccaac acttatcgta
     1321 cttatggttc gccgttctac ccgggaaatg gatatggagt tttcccaaac cagaaaaaaa
     1381 cgccagagaa tccaaatgct tatgtttatt ctattaaggt tccatcttac tttgatgatg
     1441 ttgatctgat ttcagttttc agtcattggt cggtaaatcc ttctgatggc gtcgagttag
     1501 atgtggattc acccgaaact gatgtagtat tcaagacttc cggggtagtc gttacggcta
     1561 atttctatca gtatcggcgc tccgcctttc aaggcagtat tccgattgac ctgaccttaa
     1621 attgtaatat taatgtagtc ggtgatgtaa atatcacaga agccggtaat gtagtcgtgc
     1681 aaccatcaac tgtttttaaa atagcgcaga ataagataat tagagtatac ggtgacttca
     1741 cggccagtgg aagtggaatt ttttttaaac ccgtcgatcc caattacacg atcaagaact
     1801 actggaaagg catctacaca tacagtaccg gcaaaaccgt cttgaataac gtccgcgtcg
     1861 agggtgctaa ttatggattg tacatttatt atggtagggg ctatgtgtac aattgcactt
     1921 tctacaataa taattatggt ctgcggattt tataccctaa cagttccttt aatatatatc
     1981 aatgtacttt taccaataac aatactgcgg cttattctag tagcgccaat gttctcgttg
     2041 ccaacagtgc ttttagcaat aacacttatg gcctgaaaac ttatagtagt acaggaaata
     2101 tattaagcaa tgtattttcc aataattcac aaggcatcgc aatatattct ggtagtttag
     2161 atttttccac ctcgactaaa aaccaaagtt atatcaataa tacaatccag aataacagcc
     2221 agtacggtat atatatcggt agtagcgcct atcccacact gggtgaatac tatgttgatt
     2281 cgaaaggtta cactcatggc ggattcaatt tattttcacg aggtaccgga gcttatgatg
     2341 tctatagtct gtatgcatca tctattgatg ccaggatgaa ttggtgggcg tccaacgcta
     2401 atcgatatgg taatttgagc tttaccccaa aaactggaga ggtaggataa gtgataaaaa
     2461 tgtgtaac
//
LOCUS       DAOSMP010000135         2449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_42914_length_2449_cov_11.809106, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000135 DAOSMP010000000
VERSION     DAOSMP010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42914_length_2449_cov_11.809106"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PKX09_10325"
     CDS             complement(<1..839)
                     /locus_tag="PKX09_10325"
                     /inference="COORDINATES: protein motif:HMM:NF014065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HOU18258.1"
                     /translation="MNFYNNPKITDPKGFTGAAMHAGFKRKRNDLCIIKSAVPAKCSA
                     KFTTNAVKAAPLIVAMENLPKSGNRLQALIINSGNANAGTGQQGLNDVHETLNLLSAH
                     LDIPEELILASSTGVIGHCLDMEKMRNGITHLVPHLSVEGGAACAEAILTTDTIEKSF
                     GLKFRVNGAEIQLCGITKGSGMIQPNMATTLAFVTTDADIDHDFLEKTFTTTVAKTYN
                     MITVDGHTSTNDTALIMANGLAGNQTITAGSPAGEIFENALLMLMTEMAKSIVRDGEG
                     ATK"
     gene            complement(977..2011)
                     /gene="argC"
                     /locus_tag="PKX09_10330"
     CDS             complement(977..2011)
                     /gene="argC"
                     /locus_tag="PKX09_10330"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOU18259.1"
                     /translation="MKNNAKIRVSVVGATGYAGFELVKILLRHPYVTINRLAVIAEEV
                     GLCFSDLFPALKGLCDLTCTSIDVQEIKRESDVIFFCLPHTVSMTYVPDFMGNEKLLI
                     DLSADYRLNSAELYQKTYGVAHTDSGHLPDFVYGLPEFNRAKIKSAKTIANPGCFPTG
                     IELALKPVLQAGLIETTAIFVDSKTGISGAGRTPKPAYHFPECNENLTAYKVASHQHE
                     PEIEQELTKIAGSPVDVIFVPHLVPLTRGIFSTIYTKLRQPNSQAELLTLYQNDYQAE
                     PFVRILSNNQIPQLKNVANSNFCDIGLKVIGDHLILLSAIDNLIKGASGQAVQNMNIM
                     LGYPETTGLL"
     gene            complement(2001..>2449)
                     /locus_tag="PKX09_10335"
     CDS             complement(2001..>2449)
                     /locus_tag="PKX09_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOU18260.1"
                     /translation="NKVASDLILFTMPEFNYFTLPDEFLTGSSIMPHKKNPDVLELLR
                     AKYHEVISYEMQVRNTGVNLISGYHRDLQLTKEATIRAFKTTLTSVQITTVLFENLSV
                     NRKNCRKAMTAELFATDKVYQLVKSGVPFREAYRQISQEYEGKNEK"
BASE COUNT          679 a          508 c          559 g          703 t
ORIGIN      
        1 aacttggtgg cgccttcgcc gtctcggaca atggattttg ccatttcggt catcagcatt
       61 aggagagcat tttcgaaaat ttcaccagct ggcgaaccgg cggtaattgt ttgattaccc
      121 gctaaaccgt tcgccataat cagagcggta tcattggtgc tggtgtggcc atcaacggta
      181 atcatattgt aagttttggc gacagtcgtg gtgaaagttt tttccagaaa atcgtggtca
      241 atatcggcat cagtggtgac aaaagccagc gtggtcgcca tattgggctg aatcatacct
      301 gaccctttgg taatgccgca gagctggatt tctgcgccat tgactctgaa ttttaaaccg
      361 aacgattttt cgatggtatc ggttgtcaat attgcttcgg cgcaggcggc tccgccttcc
      421 acactaagat gcggtacgag gtgggtaata ccattacgca ttttttccat atcgagacaa
      481 tgtccgatta caccagtact ggaagccaga attagttctt ccggaatatc aagatgggcg
      541 gaaagtaagt ttagagtctc gtgcacatcg ttcaaaccct gctgaccggt accggcatta
      601 gcattaccgg aattgataat caaagcctga aggcgattgc cggatttcgg taaattttcc
      661 atagcgacga tgagcggcgc ggctttgacg gcattggtgg tgaatttggc tgaacattta
      721 gccggtacag cgcttttgat tatacacagg tcattacgtt tgcgcttgaa gccggcatgc
      781 attgccgcac cagtaaaacc tttggggtcg gtgattttag gattattgta aaaattcata
      841 gcgaatccat tcattaaaaa ttgaaattga tttcattaat aaaaacctgt ccgtttcttt
      901 gtcttgatcc aaagaaatga actaaagaaa aatcaaggct attaaaaatc ggctgaatta
      961 tattcatttc taatccctat aataatccag ttgtttcagg ataaccgagc atgatgttca
     1021 tattctggac ggcctgtccg gaggcacctt tgattaaatt gtcaatcgcg cttaacagaa
     1081 tcaagtgatc gccgataact tttaaaccga tatcgcaaaa attgctattg gcaacgtttt
     1141 ttaattgggg aatctgatta tttgataaaa ttcgcacgaa aggttccgct tgatagtcat
     1201 tttgatataa agtcaataat tcggcttgag aatttggctg gcgtaattta gtgtaaattg
     1261 tgctaaaaat accacgtgtc aaaggaacaa gatgcggcac gaaaattaca tcaaccggcg
     1321 agccggctat tttggtcaat tcctgctcaa tttccggctc atgttgatgc gaggcgactt
     1381 tataggcggt caggttctca ttacattccg ggaaatggta agccggtttg ggagtacgtc
     1441 ccgcgcccga aatcccggtt ttgctatcaa cgaaaatcgc cgtggtttcg attaatccgg
     1501 cttgcaaaac cggcttcaaa gccaattcta ttccggttgg aaaacacccg ggattggcga
     1561 tggtcttagc tgatttaatt ttagcgcggt taaattccgg cagaccatag acaaaatccg
     1621 gcaaatgccc gctatcggta tgggcaacgc cataagtttt ttggtaaagt tcggccgaat
     1681 taaggcgata atcggcacta agatcgatta ataatttttc gtttcccatg aaatccggga
     1741 cgtaagtcat actaaccgta tgcggcagac aaaagaaaat tacgtcactc tcgcgtttga
     1801 tttcctgaac gtcaatcgat gtgcaagtca gatcgcaaag tcctttcagg gccggaaaaa
     1861 ggtccgaaaa acataaacca acctcttcag caattactgc caaacggttg attgttacgt
     1921 acgggtgacg caatagaatt tttaccagtt cgaaaccggc gtagccggta gcaccgacta
     1981 cagatacgcg aattttggca ttatttttca ttttttccct cgtattcctg actgatttga
     2041 cggtaagctt cgcggaaagg cacgccgctt tttactaatt gataaacctt atcggtagca
     2101 aataattcgg cggtcatagc tttccgacag tttttacggt taaccgataa attttcaaat
     2161 aaaacagtag tgatttgaac cgacgttaga gtcgttttaa atgctcggat ggtagcttcc
     2221 ttagtcaatt gcaggtcgcg atgatagccg gaaatcaggt tgacgccggt attacgaacc
     2281 tgcatttcat aagagataac ttcgtgatat ttagcccgca ataattcaag cacgtccgga
     2341 tttttcttat gcggcataat ggatgagccg gttagaaatt catccggcag agtaaaataa
     2401 ttaaactccg gcatggtgaa taaaatgaga tcggaggcaa ctttgttta
//
LOCUS       DAOSMP010000136         2449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_42915_length_2449_cov_10.571846, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000136 DAOSMP010000000
VERSION     DAOSMP010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42915_length_2449_cov_10.571846"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PKX09_10340"
     CDS             complement(<1..839)
                     /locus_tag="PKX09_10340"
                     /inference="COORDINATES: protein motif:HMM:NF014065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HOU18261.1"
                     /translation="MNFYNNPKITDPKGFTGAAMHAGFKRKRNDLCIIKSAVPAKCSA
                     KFTTNAVKAAPLIVAMENLPKSGNRLQALIINSGNANAGTGQQGLNDVHETLNLLSAH
                     LDIPEELILASSTGVIGHCLDMEKMRNGITHLVPHLSVEGGAACAEAILTTDTVEKSF
                     GVKFRVNGAEIKLCGITKGSGMIQPNMATTLAFVTTDADIDHNFLEKTFTTTVAKTYN
                     MITVDGHTSTNDTALIMANGLAGNQTITAGSPAGEIFENALLTLMTEMAKSIVRDGEG
                     ATK"
     gene            complement(977..2011)
                     /gene="argC"
                     /locus_tag="PKX09_10345"
     CDS             complement(977..2011)
                     /gene="argC"
                     /locus_tag="PKX09_10345"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOU18262.1"
                     /translation="MKNNAKIRVSVVGATGYAGFELVKILLHHPYITISRLAVIAEEV
                     GLCFSDLFPALKGLCDLTCTSIDVQEIKRESDVIFFCLPHTVSMTYVPDFMGNEKLLI
                     DLSADYRLNSAELYQKTYGIAHTDSGHLPDFVYGLPEFNRTKIKSAKTIANPGCFPTG
                     IELALKPILQAGLIETTAIFVDSKTGVSGAGRAPKPAYHFPECNENLTAYKVASHQHE
                     PEIEQELSKIAGAAVDVIFVPHLVPLTRGIFSTIYTKLRRPISQAELLTLYQNDYQAE
                     PFVRVLSNNQIPQLKNVANSNFCDIGLKVIGDHLILLSAIDNLIKGASGQAVQNMNIM
                     LGYPETTGLL"
     gene            complement(1995..>2449)
                     /locus_tag="PKX09_10350"
     CDS             complement(1995..>2449)
                     /locus_tag="PKX09_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOU18263.1"
                     /translation="NKVASDLILFTMPEFNYFTLPDEFLTGSSIMPHKKNPDVLELLR
                     AKYNEVFTYEMQVRNTGVNLISGYHRDLQLTKEAIIRAFKTTLTSVQITTVLFKNLSV
                     NHKNCRKAMTAELFATDKVYLLVKSGVPFREAYRQISQEYEDKNEKQC"
BASE COUNT          669 a          509 c          570 g          701 t
ORIGIN      
        1 aacttggtgg cgccttcgcc gtctcggaca atggattttg ccatttcggt catcaacgtt
       61 aggagagcat tttcgaaaat ttcaccagct ggcgaaccgg cggtaattgt ctggttgccc
      121 gctaaaccgt tcgccataat cagggcagta tcgttggtgc tggtgtggcc atcaacggta
      181 atcatattgt aagtttttgc gacagtcgtg gtgaaagtct tttccagaaa attgtggtca
      241 atatcggcat cagtggtgac aaaagccagt gtggtcgcca tattaggctg aatcataccg
      301 gaacctttgg taatgccgca aagcttgatt tccgcgccat tgactctgaa ttttacaccg
      361 aacgattttt cgacggtatc ggtcgtcaat atcgcttcgg cgcaggcggc tccgccttcc
      421 acactaagat gcggtacgag gtgggtaata ccattacgca ttttttccat atcgagacaa
      481 tgtccgatta caccagtact ggaagccaga attagttctt ccggaatatc aagatgggcg
      541 gaaagtaagt ttagagtctc gtgcacatcg ttcaaaccct gctgaccggt accggcatta
      601 gcattaccgg aattgataat caaagcctga aggcgattgc cggatttcgg taaattttcc
      661 atagcgacga tgagcggcgc ggctttgacg gcattggtgg tgaatttggc tgaacattta
      721 gccggtacag cgcttttgat tatacacagg tcattacgtt tgcgcttgaa gccggcatgc
      781 attgccgcac cagtaaaacc tttggggtcg gtgattttag gattattgta aaaattcata
      841 gcgaatccat tcattaaaaa ttgaaattga tttcattaat aaaaacctgt ccgtttcttt
      901 gtcttgatcc aaagaaatga actaaagaaa aatcaaggct attaaaaatc ggctgaatta
      961 tattcatttc taatccctat aataatccag ttgtttcagg ataaccgagc atgatgttca
     1021 tattctggac ggcctgtccg gaggcacctt tgattaaatt gtcaatcgcg cttagcagaa
     1081 tcaagtgatc gccgataact tttaaaccga tatcgcaaaa attgctatta gcaacgtttt
     1141 ttaattgggg gatttggtta tttgatagaa cgcgcacgaa aggttccgct tgatagtcat
     1201 tttgatataa ggttaataat tcggcttgag aaattggccg ccgcaatttg gtgtaaatcg
     1261 tgctaaaaat accccgcgtc aaaggaacaa gatgcggcac gaaaatcaca tcaaccgccg
     1321 cgcccgcaat tttgctcaat tcctgttcga tttccggttc atgttgatgc gatgcgactt
     1381 tataggcggt caggttctca ttacattccg ggaagtgata agccggtttg ggagcgcgtc
     1441 cggcgccgga gacaccggtt ttgctatcca cgaaaatcgc cgtagtttcg attaatccgg
     1501 cttgcaaaat tggcttcaaa gccagttcta ttccagttgg aaaacatccg ggattagcga
     1561 tggttttagc tgatttaatt ttagtgcggt taaattccgg cagaccatag acaaaatccg
     1621 gcaaatgccc gctatcggtg tgggcgatgc cataagtttt ttggtaaagt tcggccgaat
     1681 taaggcgata atcggcacta agatcgatta ataatttttc gtttcccatg aaatccggga
     1741 cgtaagtcat actaaccgta tgcggcagac aaaagaaaat tacgtcactc tcgcgtttga
     1801 tttcctgaac gtcaatcgaa gtgcaggtca gatcgcaaag tcctttcaat gccggaaaaa
     1861 ggtccgaaaa acacaaaccg acttcttcgg cgatgacagc cagacggctg atcgttatgt
     1921 acggatggtg tagcagaatt tttaccagtt cgaaaccggc gtagccggta gcaccgacta
     1981 cagatacacg aattttagca ttgtttttca tttttatcct catattcctg actgatttga
     2041 cggtaagctt cgcggaaggg cacaccgctt tttactaata gataaacctt atcggtagca
     2101 aataattcgg cggtcatagc tttccgacag tttttatggt taaccgataa atttttaaat
     2161 aaaacagtag tgatttgaac cgacgttagc gtcgttttaa aagctcggat gatggcttct
     2221 ttggtcaatt gcaggtcgcg atgatagccg gaaatcaggt tgacgccggt attgcgaacc
     2281 tgcatttcat aagtgaaaac ctcgttatat ttagcccgta ataattcaag tacatccgga
     2341 tttttcttat gcggcataat ggatgagccg gttagaaatt catccggaag ggtgaaataa
     2401 ttaaactccg gcatggtgaa taaaatgaga tcggaggcaa ctttgttta
//
LOCUS       DAOSMP010000137         2432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_43280_length_2432_cov_3.800168, whole genome shotgun sequence.
ACCESSION   DAOSMP010000137 DAOSMP010000000
VERSION     DAOSMP010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43280_length_2432_cov_3.800168"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2432)
                     /locus_tag="PKX09_10355"
     CDS             complement(<1..>2432)
                     /locus_tag="PKX09_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18264.1"
                     /translation="FSAPGWNGVANNGILDKDRIVGWHIIIQTENTAIGDFVSGTTLF
                     DQLQLETGEIVGEFSVTFQADLTDFIANGWFNPANPADSIVVNGDFNGWDSHQKMLPS
                     ESNPNIYLFTTVVTGNPGDIVGWKFRIFPDSKWTNNGWESIENRELILRENNIVLESI
                     KPIIYYRGNPITQDVTVTFTVDMSNFDGSFSAVSLQGNALPLNWSPGSLPMDDSDKDG
                     IYTVAVFFPSGTGKTIEYKYTALTDTAWVWESFVGNRSFEIDDSSPDQILAVDIFNGY
                     GTALNASVYLNGQKARLRVTDSAPINPAANPSAYVITGKAITVETWVFPLNLPENGNC
                     AVLVSRPYWNTDPWYAYELRINNLGPTDEPRFDFVVSDGNPGGTHNGVISSNPITIGE
                     WTHVVGTYDGSNLHLYVNGELVGEASFTANIGQGDAGFYLGGTWSNYFDGVIDEVRLW
                     NVCRSQADIQNTMNQQLVGDEAGLVGYWPLDEEIEVNGSYPVCADITTNHNDLLMQNG
                     ARIISASAPLVEPEIAPEFQIQELYGIANINFSYQILCSGWHKPTFTLVNGPQGLILN
                     NGWVYWTPTTAGHYSLVLEARNSAGVTQGIYHIWVDSILVSTDTHNNNNVLLNVYNNG
                     FIGNTLPDLDGNGFQYNGTNGLFIGSLIIAQSANQVAGKIYNNEFGTLEPVHSVTSML
                     TGFDQAFESRFNDTRKLNPIGVEVVQRTHSKSTNPDQDYVLVEYEIYNKSGRQLTDLY
                     IGLAMDWDVGNDATHNFSGYDAEKKLSYAFLNNETPYFGITALTGDVTGYKTWLANSG
                     EDENSDEFLFQA"
BASE COUNT          715 a          551 c          473 g          693 t
ORIGIN      
        1 ttgcctgaaa taggaattca tcggaatttt catcctctcc tgaattagcc agccatgttt
       61 tataaccggt aacatccccg gttagagcag ttattccaaa ataaggggtt tcattattta
      121 aaaaagcata gctaagtttt ttctcggcat catacccgct aaaattgtgg gtggcgtcgt
      181 ttccaacatc ccaatccatt gctagaccaa tatacaaatc cgttaattgc ctccccgact
      241 tgttgtaaat ttcatattcc actaaaacgt aatcctgatc aggattggta gatttggaat
      301 gagttctttg gacaacttcg acaccgatag gatttaattt ccgtgtatca ttaaaacgac
      361 tttcaaaagc ctgatcgaag cctgtcaaca tacttgttac agaatggacc ggttccagag
      421 ttccaaattc gttattgtaa attttccccg caacctgatt cgcactctga gcaataataa
      481 gactacctat gaatagcccg tttgtgccat tatattgaaa gccgttgccg tcaaggtcgg
      541 gtagagtatt gccgataaat ccgttattgt aaacgtttag taatacatta ttattgttgt
      601 gagtatctgt acttactagt atcgaatcta cccagatatg gtagatccct tgagttacac
      661 cggctgaatt tctggcttcc agaactaacg agtagtgtcc ggcagtagta ggtgtccaat
      721 acacccatcc attgtttaga attaagccct gaggaccgtt gacaagcgta aaggttggct
      781 tatgccatcc ggaacagagt atttgatatg agaaatttat attcgctatc ccgtagagtt
      841 cttgaatttg gaactcagga gcaatttctg gttctactaa tggagccgaa gccgatataa
      901 tccgagctcc attctgcatt agaagatcgt tatgattagt agtaatatcg gcacagactg
      961 gatatgaacc atttacttca atctcttcat caagaggcca atagccaact agcccggctt
     1021 catcacccac gagctgctgg ttcattgtat tctgaatatc tgcttgacta cgacagacat
     1081 tccagagtct tacttcatct ataacaccgt caaaataatt actccaggtg ccaccgagat
     1141 aaaatcctgc atctccttgt ccgatattgg cagtaaaaga tgcttcgccg acaagttcac
     1201 cgttaacata gaggtgtaga ttactgccgt cataagttcc aactacatgg gtccactcgc
     1261 cgatcgtaat cggattcgaa ctgatgaccc cattatgagt accgccagga ttgccatcgc
     1321 taacaacgaa atcgaagcgc ggctcatcgg tcggaccgag attgttgatc cggagttcat
     1381 aagcgtacca ggggtctgta ttccaataag gacgcgaaac cagcacagca caattaccat
     1441 tttcaggtaa attcagcggg aagacccagg tttcgaccgt tatggctttt ccggtaatta
     1501 cataggcgct gggattggct gccggattga ttggagcact gtcggtcaca cgtagtctgg
     1561 ctttctgacc atttaaataa accgatgcgt tgagagcagt accatatcca ttaaaaatat
     1621 caacggctag tatttggtcg ggtgaactat catcaatctc aaaactgcga ttgccgacaa
     1681 aactttccca gacccaagct gtatcggtaa gcgctgtata tttatactct atagttttgc
     1741 cagtcccact tggaaaaaat acagccactg tataaatacc atctttatcg gaatcatcca
     1801 ttggtaacga gccgggcgac caattcaatg gtaaagcatt gccttgcaga gagactgcac
     1861 taaaactacc gtcaaagtta gacatatcaa ctgtaaatgt aacggttaca tcctgcgtaa
     1921 tgggattacc acgatagtaa atgatcggtt taattgattc caagacaata ttattttctc
     1981 ttaatattaa ttctcggttt tcaatacttt cccaaccatt attagtccat ttagaatcag
     2041 ggaaaattct gaatttccaa ccgacgatat cgccgggatt accagttaca acggttgtaa
     2101 agaggtaaat attcggattg ctttcgctgg gcaacatttt ctgatgagaa tcccagccat
     2161 tgaaatcgcc attaaccaca atagaatcag ctggatttgc gggattaaac cagccattag
     2221 caataaagtc ggttaagtca gcctggaagg ttaccgaaaa ctcaccgaca atctccccag
     2281 tttctaactg caactggtca aaaagagtcg tcccactaac aaaatcacct atagcggtat
     2341 tttcagtctg aataataata tgccagccaa cgattcgatc tttatccaaa ataccattgt
     2401 tagctactcc attccagccc ggcgcactaa aa
//
LOCUS       DAOSMP010000138         2411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_43805_length_2411_cov_3.333192, whole genome shotgun sequence.
ACCESSION   DAOSMP010000138 DAOSMP010000000
VERSION     DAOSMP010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43805_length_2411_cov_3.333192"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PKX09_10360"
     CDS             complement(<1..494)
                     /locus_tag="PKX09_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3308 domain-containing protein"
                     /protein_id="HOU18265.1"
                     /translation="MKTIFKSIIIGIVVGCLTIPVELSAKNEKLAQAGFEFLSVVSDA
                     RAAAMADAMTSLQIGSSALFFNPAGMANIEQLIDLTASNNQWITDIQHNTFSLAFSPT
                     HGRYGVIGLSYQNVNYGDFYGTRVNMRVDAGYDDTGVFQLSAHALGLGYAKRLTEWFS
                     VGGQ"
     gene            complement(508..>2411)
                     /locus_tag="PKX09_10365"
     CDS             complement(508..>2411)
                     /locus_tag="PKX09_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18266.1"
                     /translation="RHSHPVVAVDNQTGTNNLLYDQVDDYDSNLPCDRMVVAEFNTSM
                     GVSVTRKVYGFTQPDHDNYFIQEYVFKNTGIYNENGDVHHQDLQDFWVYLCWRPAFAG
                     VTSSGWTDQPAWGAFASEWGSSTLIHTFNVNSPEGMRGFYSYYGPTNDSNHPLTPAED
                     WGCPDHLETGVLGSAKYLGGVVLFASNPNNHMIDDPSQPATDAYVGSDGVPMETNVSQ
                     YDISFMQQRYNIMIEGHLPASQDENFFNSVGHNGYVEEWTTGNTYRNTGTKGGSSQGW
                     GFGPYYLADGDSIRIVFAIGVTGLSWEKCREVGTNWYAYYKSLTNKPDLYMPDGSPAS
                     ATNPWGYAAHNAYKRAWCETGRDSILQVLSNAIDNYNAGYIIPQPPPPPESFTVTSGG
                     DRIRLTWSDNAVNSPNFNGYVIYRSEGNVKHYKTVYTKIFECDKASCVHEFDDVTAAR
                     GNNYYYYIQSKSDGQENNGKPLYSSLFYTLTSVAAYLRRPAGNFLEHVRVVPNPYDIR
                     ARMWQFGEDFQYDRIAFYELPPICKVKVFTERGDLIWEKYHDNGSGDELWDSMTSSRQ
                     IIVSGIYILYVEVPQDIYAEQDIVAARDYYNPKTGEKEISKGEVIFRQGDKMFNKGDS
                     VFRKFVVIR"
BASE COUNT          671 a          594 c          485 g          661 t
ORIGIN      
        1 atttgaccgc ccacactgaa ccactccgtc aagcgcttcg cataacccag tcctagggcg
       61 tgggcactca gctgaaagac gccggtgtca tcatagccag cgtcaactct catatttacc
      121 cgcgtgccat aaaaatcccc ataattcaca ttctggtaac ttaagcctat cactccatat
      181 cgtccatgag tcggactaaa cgccagacta aaagtattat gttgaatatc cgttatccac
      241 tgattattac tggcagttag gtcaattaat tgctcgatat tagccatacc tgccggatta
      301 aagaatagag cgcttgaacc gatctgaaga cttgtcattg catccgccat cgccgccgct
      361 cgagcatcgg ataccacact gagaaattca aaaccagctt gagccaattt ttcgtttttg
      421 gcagataatt cgacaggtat tgtcaaacat ccaactacga ttccaataat tatagatttg
      481 aatattgttt tcatattccg cccctcctta cctaatcacc acgaatttgc gaaataccga
      541 atcgcctttg ttgaacatct tgtcaccttg ccgaaaaatc acctcgccct tgctaatttc
      601 tttttccccg gttttcggat tgtagtaatc acgggcggct acgatatcct gttcggcata
      661 gatgtcctgc ggtacttcca cgtaaaggat atatatacca ctgacaataa tctgacggga
      721 agaagtcatt gaatcccata attcgtcgcc ggaaccgtta tcgtgatatt tttcccagat
      781 taggtcgccc ctttcagtaa aaacctttac cttgcagata ggcggtaatt catagaaagc
      841 aattcgatca tactggaaat cctcgccaaa ttgccacatt cgagcgcgaa tgtcgtaggg
      901 attcggcaca acacgtacgt gttccaaaaa gtttccagct gggcgacgca gataagccgc
      961 tacgctggtc agtgtataaa agagactgct gtatagcggt ttgccattgt tttcttgacc
     1021 gtcactctta gattggatgt agtaatagta attattaccg cgagcggcgg tgacatcgtc
     1081 gaactcatgt acgcaacttg ctttatcgca ttcaaaaatt tttgtataga cggttttata
     1141 atgtttaaca ttgccttccg agcgatagat aacatatcca ttaaaattag gcgaatttac
     1201 ggcattgtca ctccaggtca agcgaatccg atctccacca gaagtaaccg taaatgattc
     1261 gggtggggga ggcggttgcg gaataatgta tcccgcattg taattatcta tagcattact
     1321 taggacttgc agaatcgaat cgcggccggt ctcgcaccaa gctcgtttat aggcgttatg
     1381 ggccgcatag ccccagggat tggtagctga tgctggagaa ccgtcgggca tatacaaatc
     1441 cggtttgtta gttagacttt tataataggc ataccagtta gttccgactt ctcgacattt
     1501 ttcccagctt aatccggtca ctccgatggc aaatacgatt cggatgctat cgccatcagc
     1561 caaataataa gggccaaatc cccatccttg actgctaccg cctttggtac cggtgtttcg
     1621 gtaagtattc ccagtagtcc attcctccac ataaccattg tggccaacag aattgaaaaa
     1681 gttttcatcc tggctagcag gcaaatgccc ttcgatcata atgttatacc gttgttgcat
     1741 aaaactgata tcatattgac tcacattagt ctccattgga acgccatcgc tgccaacata
     1801 ggcatccgta gctggctggc tggggtcatc aatcatatga ttattcggat tcgatgcaaa
     1861 tagaacgaca cctcctaaat acttagccga accaagcaca ccggtctcta aatgatcagg
     1921 acacccccaa tcctcagccg gagttagtgg atgattagaa tcattagtgg gaccataata
     1981 actataaaat cctcgcatcc cttcaggaga attgacgttg aatgtatgaa tgagagtact
     2041 gctaccccat tccgaggcaa aagcgcccca agccggttga tcagtccagc cggatgaagt
     2101 cactccggca aatgccggcc gccaacataa ataaacccag aaatcttgca gatcctgatg
     2161 atgtacgtcg ccattttcgt tatatatgcc cgtattttta aatacatatt cctgaataaa
     2221 ataattatcg tgatccggct gagtgaaacc ataaactttt ctggttaccg atacacccat
     2281 cgaagtattg aactcggcaa ctaccatccg gtcacatggc aggttactat catagtcatc
     2341 aacctgatca tagagaagat tattagtgcc tgtttgatta tccacagcaa ccacagggtg
     2401 actatgtcgc c
//
LOCUS       DAOSMP010000139         2387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_44422_length_2387_cov_12.708405, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000139 DAOSMP010000000
VERSION     DAOSMP010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44422_length_2387_cov_12.708405"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PKX09_10370"
     CDS             <1..689
                     /locus_tag="PKX09_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18267.1"
                     /translation="NGGSSLIMLDPRPAPGLSDYMKGWKIDVGDNLIIDASGFGRLFG
                     ASPDIPLVTDYTDHPIVKDMKNVMTFFPMARTVSSLELDDEDNITVAELLRTGQQSFA
                     VANIEEVYRTGRVSRGPNDLQGPLSVACAATIKTTSGQRNGRLVVVGDSDFASNAYFD
                     NQANGDLFMNMISWLIADEDLISIRPKNPEISTVDMTPGQTKIVMWLTVIILPAIAFG
                     VAIMVYVRRK"
     gene            693..2063
                     /locus_tag="PKX09_10375"
     CDS             693..2063
                     /locus_tag="PKX09_10375"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOU18268.1"
                     /translation="MKKGRSTLILFGILVVLFAAIYFFEIKGGEKRAKKSKLANQLFQ
                     AEKDSVKQLILSGDYGDITCEKKGMEWIITTPVLTQGDAQSIESNLDLILGANIERKI
                     ADTTDDLSLFGLLEPRGKVQVITQNGKHTIVLIGDENPTGDLIFVKYPDDNSVYTTNK
                     ALWTNVNKKLYDLRDKKIMHFNMDEVRKINITSRAKGKVSLEKVAGKWQIVDPVNLPA
                     NDNEVKSFLTRLSTGKAKEFIDEEPDDLKKYGLTNPAVMITLELGESSARTSFVVGDS
                     AKIDGGGFYAKEETRRPVFSIEKWTVDGITKSAFDLQDKEILGFDSQKCERIVWQIAN
                     QEYIATKIDSVNWMFVSPETLQVDENLMRRWLEALRDFSVDELESYQPKSLSVYGLDK
                     PTLRLAFYDNDSKLGELLIGKEISEKYYVKTSNYPHVYRIRKNTFEQIYKKPEDLMIK
                     PVSTES"
     gene            complement(2141..>2387)
                     /locus_tag="PKX09_10380"
     CDS             complement(2141..>2387)
                     /locus_tag="PKX09_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18269.1"
                     /translation="RPKIKNPFRNLHINFRPKKSKKTRDREQAPISYETEETMREELD
                     HILDKISTEGYDHLSEQERSTLYLLSKYFAEKDRRKS"
BASE COUNT          694 a          463 c          558 g          672 t
ORIGIN      
        1 aaaacggcgg tagtagcctg ataatgctgg atccgcgtcc tgcacccggt ttgagcgatt
       61 atatgaaagg gtggaagatt gatgttggcg ataatttaat tattgatgcc agtggcttcg
      121 gtcgcctttt tggcgccagc ccggatatac cactcgttac cgattatacc gatcatccca
      181 tcgttaagga tatgaaaaat gtaatgacct tcttcccaat ggccagaaca gttagttcgc
      241 tggaattgga tgatgaagat aacataacgg tagcggaatt actccggacc ggtcagcaga
      301 gttttgcggt tgccaatatc gaggaagtgt atcgtaccgg ccgcgtttcc agaggaccga
      361 atgatctgca gggtcctctt tcagtcgcct gcgcggcgac aattaagacc acttccggtc
      421 agcgtaacgg tcggctagtg gtggtggggg attccgattt tgcctcgaat gcctatttcg
      481 ataatcaagc taatggcgat ttatttatga atatgatcag ctggcttatt gccgacgaag
      541 acctgatttc gatccgtccc aaaaatccgg aaattagtac ggttgatatg actcccgggc
      601 agaccaaaat agttatgtgg ctgacggtta taattttacc ggcaattgct tttggcgtgg
      661 cgataatggt ttatgtcagg aggaaataag ttatgaagaa aggccgatca accctgattt
      721 tatttggcat tctggtcgtc ttatttgctg ctatttattt ttttgaaatt aaaggcggtg
      781 aaaagcgcgc aaagaaaagc aaactggcca atcaactatt ccaggccgag aaagacagcg
      841 tcaaacaatt gattctttcc ggagactacg gagatattac ttgtgagaaa aaagggatgg
      901 aatggatcat caccacaccg gttttgactc agggcgatgc ccagagtatc gaatctaatc
      961 tcgatttaat tctaggtgct aatatcgaac gcaaaatcgc cgacacaact gacgatttaa
     1021 gcctgttcgg tttattagaa ccgcgcggaa aagtgcaggt tattacccaa aacggcaaac
     1081 ataccatagt tttaatcggc gacgaaaatc cgaccggcga cttgattttt gtcaaatatc
     1141 cggatgacaa tagcgtttat actaccaata aagcactctg gactaacgtt aataaaaagc
     1201 tatacgattt gcgtgataag aagattatgc acttcaatat ggatgaagta cgcaaaatca
     1261 atatcacttc ccgggcaaaa ggaaaagtca gtctggaaaa agtcgcggga aaatggcaaa
     1321 tcgttgaccc agtaaatcta ccggcaaatg ataatgaagt caaatcattt ttaactcgcc
     1381 tttcaaccgg caaagcgaag gagtttattg acgaagaacc ggatgatctc aaaaaatatg
     1441 gattgaccaa tccagccgtt atgattactc ttgaactggg tgaaagctcg gcgcgtacca
     1501 gttttgtagt cggcgatagt gcaaaaattg acggtggcgg attttacgct aaggaagaaa
     1561 cccgccgacc ggtttttagt atcgaaaaat ggacagtaga cggtattact aaaagtgctt
     1621 tcgatttgca ggataaagaa atcctcgggt tcgatagtca gaaatgtgag cggatcgtct
     1681 ggcagattgc taatcaggaa tacattgcta caaaaattga ttcagtcaat tggatgtttg
     1741 tatcaccgga gactctacaa gttgatgaaa acttgatgcg ccgctggctg gaagctttgc
     1801 gggattttag cgttgacgag ctggaatctt atcaacctaa atcgctatcc gtgtatgggc
     1861 tggataagcc cactttaagg ctggctttct atgataatga ttccaaactg ggtgaacttt
     1921 taatcggcaa agagatttcc gagaagtatt atgtaaaaac cagtaattat ccgcacgttt
     1981 atcgaatcag gaaaaacacc ttcgaacaga tttataaaaa gccagaagat ttgatgatta
     2041 aacccgtttc tacggaaagt taatatcaat caatttacga attcccttcc tttgcgggac
     2101 tactttagga gaattcatcc gcatttttta gagtaaccat ttaactttta cgtcggtctt
     2161 tttcggcaaa atatttactt agtaggtata aagtcgagcg ctcctgctcg gaaaggtgat
     2221 cgtagccttc agtgctgatt ttatctaaaa tgtgatcaag ttcctcacgc atagtctctt
     2281 cggtttcata agagataggt gcttgttctc tgtctctggt cttttttgat tttttcggcc
     2341 ggaaattaat atgcagattg cggaagggat ttttgatttt ggggcgt
//
LOCUS       DAOSMP010000140         2365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_45015_length_2365_cov_24.574026, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000140 DAOSMP010000000
VERSION     DAOSMP010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45015_length_2365_cov_24.574026"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1457)
                     /locus_tag="PKX09_10385"
     CDS             complement(96..1457)
                     /locus_tag="PKX09_10385"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU18270.1"
                     /translation="MDKKENLPIVIYGGPLFDGINTVYEDGAVYICNGIVEKVGNESE
                     VFPQVTNTEQVEIYDTQGKVIFPGLINLHHHFYAALAKGLSPVGPVTNFQEYLENLWW
                     RFDRALDPEAVQLAVLLSLLDCIKSGVTTIFDHHSSPSHVKGSLDNIAAVVERSGLRA
                     CLCYEISDRNGRKTFHDSLQENLAFIESHRDQRRLQGNLGLHVNFTLSEKSMAEIAQY
                     YDPEIGINIHCAEDKIDREYCRELGYDGPVSRLKHFGLLGKKSILAHGVHLDQAELTE
                     VARSGSIIVHNPESNADNSVGRLNLPPADDVSIGLGTDGMTSAMLATCRAGYLIQRQA
                     GVADTLILNYLPQCLFKTNSTFASQLIGFQLGQIVAGAAADITVFDYIPVTPFNSDNI
                     FRHIIYGMPSARADMVMVDGQQIYTDGAFLTLDEELIREEAQKASQRVWKQFAETNVL
                     HFK"
     gene            complement(1688..>2365)
                     /locus_tag="PKX09_10390"
     CDS             complement(1688..>2365)
                     /locus_tag="PKX09_10390"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOU18271.1"
                     /translation="QNFSTDLTAACRDKPDWIIIAVTDDAIEEIATQISQCLPDFKAV
                     KVFHTSGFLSSEALSSLKRKGAATGSLHPVVSVPDITTGIRLLGQCVYTCEGVIKKSL
                     AEIVRSIGGQPYLLNRQQKETIHLSAVFLNNYVVALINAIKILNSDSNLEPSKAQAIL
                     QPITEQAVLSGWEKSATDALTGPLMRGDYQTIEKHLALLDNYPELKKLYQDFIDLALQ
                     VVAHPKL"
BASE COUNT          685 a          532 c          479 g          669 t
ORIGIN      
        1 actgcttctt ttttacgcag atttacgacg ataatcataa aacttttagt catagatcct
       61 cctgatactt tagcaatata ttgttgaact tattatcatt taaaatgtaa cacattggtt
      121 tcagcgaatt gtttccaaac tctttgactg gctttctggg cttcttcacg aattaattct
      181 tcatcgagcg ttaaaaatgc tccgtcggtg tagatttgtt gaccatccac cataaccata
      241 tccgcgcgag cgctgggcat tccataaatg atatgacgga aaatattgtc cgagttaaac
      301 ggcgtaaccg gtatataatc gaaaaccgta atatccgccg ccgcacctgc tacaatttga
      361 cctaattgga agccgatcaa ttgactggca aacgtggaat tggttttgaa taaacattgc
      421 ggtaaataat tgaggattag agtgtcggca actccagctt ggcgttggat taaataaccg
      481 gcgcgacagg tcgccagcat cgcggaagtc atcccatccg tacccagtcc aatcgaaacg
      541 tcatctgccg ggggtaaatt caatcgcccg acggaattat cggcattcga ttcgggatta
      601 tgtacaataa tactaccact ccgagcaact tcggtcagtt ctgcttgatc gaggtgaacg
      661 ccgtgcgcca gaatggattt tttacccagt aatccaaaat gttttagccg actgaccggt
      721 ccatcataac ctaattcacg acaatattcc cgatctattt tatcctctgc acaatgaata
      781 ttaataccaa tttccgggtc gtagtattga gcaatttcag ccattgattt ttctgataaa
      841 gtaaaattga catgcaagcc gagatttccc tgtaatcgtc gctgatcgcg atgagattcg
      901 ataaaggcaa ggttttcctg taagctgtca tggaaagttt tccggccgtt gcggtcagaa
      961 atttcatagc aaagacaagc ccgcaagcca ctccgctcga cgactgcggc gatgttatcg
     1021 agactgcctt tgacgtgcga cggcgatgag tggtgatcaa aaatcgtagt taccccggat
     1081 ttaatgcagt ccaacagaga caaaagcacc gctaattgaa ctgcttcggg atcaagcgcc
     1141 cggtcgaaac gccaccacaa gttctccaaa tattcttgaa aatttgttac cggccctact
     1201 ggagaaagcc ctttagccag cgccgcataa aaatgatgat gcagattaat taatcccgga
     1261 aaaattactt tgccctgagt atcgtaaatt tcgacctgtt cggtattagt aacttgggga
     1321 aacacctcag attcattgcc aactttctcg actatcccat tgcaaatata cacagctccg
     1381 tcttcataaa ccgtgttgat accatcaaat agtggtccac cataaataac aattggaaga
     1441 ttttcttttt tatccataaa cgcctctgct aactgtgttc aataaattag aataatgtgc
     1501 caccattagt atttaaagct aacttaccgt ttaagactgg ctggaaattc aatttgtact
     1561 ttaaaccttc taatataatc acgatataaa atagtagaat ctatcgaaca ttcaaaaaat
     1621 taattctctt caagagccgg aatcgggatt attcgaaatc ccattaaaat caatttaaac
     1681 aaaatattta caatttcggg tgagctacta cctgaagcgc cagatcaatg aaatcctgat
     1741 ataatttctt tagttccggg taattatcca gtaaagcaag atgtttttcg atcgtttggt
     1801 agtcgccacg cataagaggt ccagtcaggg catcggtagc gcttttttcc caaccgctga
     1861 ggacagcttg ctcggtaatc ggttgtagaa tcgcctgagc ttttgacggc tcaagattac
     1921 tgtcactgtt cagtatttta atggcattaa ttaaagcgac tacataatta ttcaagaaaa
     1981 cggctgatag gtggatggtc tctttttgct gacgattaag caggtaaggc tgtccaccga
     2041 ttgacctaac gatttctgct aaggatttct taattacgcc ttcgcaagta taaacgcact
     2101 gaccaagcag gcgaatgccc gttgttatgt ctggcacact gacaaccggg tgcaaagaac
     2161 cagttgcggc gcccttgcgt ttcagactgc ttaacgcctc cgaacttaga aaaccggaag
     2221 tgtgaaagac cttgaccgcc ttaaaatccg gaaggcattg acttatttga gtcgcaatct
     2281 cctcgatagc gtcgtcggta acagcgatga taatccagtc cggcttatca cggcaagcgg
     2341 ctgtcaaatc ggttgaaaaa ttctg
//
LOCUS       DAOSMP010000141         2357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_45206_length_2357_cov_14.872719, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000141 DAOSMP010000000
VERSION     DAOSMP010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45206_length_2357_cov_14.872719"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..224)
                     /locus_tag="PKX09_10395"
     tRNA            complement(151..224)
                     /locus_tag="PKX09_10395"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(188..190),aa:Arg,seq:tct)
     gene            complement(248..323)
                     /locus_tag="PKX09_10400"
     tRNA            complement(248..323)
                     /locus_tag="PKX09_10400"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(285..287),aa:Pro,seq:tgg)
     gene            505..799
                     /locus_tag="PKX09_10405"
                     /pseudo
     CDS             505..799
                     /locus_tag="PKX09_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950358.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(946..>2357)
                     /locus_tag="PKX09_10410"
     CDS             complement(946..>2357)
                     /locus_tag="PKX09_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOU18272.1"
                     /translation="LNKQITQLSTAEIINQIVEMTNFTALLAGLPDGTQKIANVKKLI
                     ELALEWEKAESLSPIDFIRRIQIYRDKEVREGEANLSAAKGDAVTIMTIHAAKGLDSQ
                     IVFLPLLADKINYRSDRFLFHSDIGAAVAIKAVKNQSHPFHYNQLKHLERRRTLAEEK
                     RLLYVAMTRARSYLVCSATVSGKSDEPDKSLWDLSAEIFVRAHEKNLCRIVKLTKTEI
                     EQTALPSGISLVQPVELNDETLRRIRTQIQPLEFKPPIRNLTATALAEWVVAGEKIAS
                     TAEPEDDQALSPQELGTLIHKAFSWWDFQSLDSFQNMLADLLRPYRLTAPESQKISAE
                     LSDWVKQLLDSDNHLRQRLTSARNRRREVEVVGLYGEMILEGKIDLLIENDDNTLTII
                     DFKSDRIAEKPDEVLLKKYSTQLDFYAFILERCSKRKVREQALYFIRNGLLITKTISE
                     SIVNDAEKNIRKYITESAI"
BASE COUNT          660 a          486 c          522 g          689 t
ORIGIN      
        1 tgcgatcgaa gtttgattta agattgtgca ttagtttatc tccttgttta ttattgaatt
       61 acaatacaat aatttaagga tttttaagct aatgcacatc ttatttttta ggtttttatt
      121 tcacacaacg ggttaagtaa aatatgcgcg taccccgagc aggactcgaa cctgcaacca
      181 cctgcttaga aggcagatgc tctatccact tgagctatcg gggcattagt aaaaatcaga
      241 aagatcgtcg gggtgagtgg atttgaacca ccggccccct gctcccaaag caggtgcgct
      301 aaaccggact gcgctacacc ccgttctcca aattctatct ctgattatga aaaaacaatc
      361 actttaagtt attataatga acttgattat caatggataa aaaaaagcac gtaaagttag
      421 gatattaaag tcattaaagt caagcctgaa aaaattgttg cctttaataa aataatcaca
      481 tttaatattg aggtatagaa ggaagtgaat actgtctatc gggcgttatc cgatccaacc
      541 cgacgacaaa ttctgaaatt attgcgcaag aaaaacttga ccgccggtga aatcgccgga
      601 cattttacca caacgaaaac ttctctgtcg aagcacttta gcattttaca gtcatttggg
      661 ccctagaccg attcaaggcg aaaaattggg cgtcacaatt ccttaccatt tgaaagtcag
      721 tgtacttgaa aaagccaata tggcattgat ggaaatattt aaactaaagg ggaaagataa
      781 tggaagacaa tcggagtaaa atcgagcttc tgcgacctcg acgcagttct gttcatttct
      841 gcacttatgc aaaaatttga attagtaaaa tagcagactg caaagggcgc gaagaagatc
      901 actattttct ttttcttgga gggaatttgg gtatttatgg caagcttata ttgccgattc
      961 agtaatatat ttacgaatat ttttttcggc gtcgttcact attgattcgg atattgtttt
     1021 agtaatcaat aaaccattac gtatgaagta aagagcttgt tctctaactt tccgcttgga
     1081 acaacgttcg agtatgaaag cataaaaatc cagctgggtg gagtactttt tcaatagaac
     1141 ttcgtccggt ttctctgcaa tgcggtcgga tttgaagtct ataatagtaa gagtattgtc
     1201 gtcattttcg atgagcagat caattttacc ctcgaggatc atttctccgt aaagcccgac
     1261 cacctcgact tcccgtcgcc gatttctggc ggaagtcaat cgctggcgta aatgattatc
     1321 agaatcgagc aattgcttaa cccagtcgga aagttcagcg ctaattttct gcgactcagg
     1381 tgcggttagt cggtagggac gcagtaaatc agccagcata ttttgaaacg aatccagcga
     1441 ctgaaaatcc caccagctga aggctttgtg aatcaaagtg cctaactcct gtggtgaaag
     1501 cgcctggtcg tcttctggct cggcggtgga ggcgattttc tcaccggcga ctacccattc
     1561 tgccagagcg gtagcagtta aattccggat cggcggtttg aactcgagcg gttgaatttg
     1621 agtgcgaatt ctccgcaacg tttcatcgtt taattcgact ggctgaacga gtgaaatccc
     1681 actggggaga gccgtctgtt cgatttcggt tttggttaat tttacgatgc ggcacagatt
     1741 tttttcatgc gccctaacaa aaatttcggc acttaaatcc cagagcgatt tatccggttc
     1801 gtcggatttg ccggatacag ttgccgagca gaccaaatac gaacgggcgc gagtcatagc
     1861 gacgtacagc aggcgttttt cttcggctaa agtccggcgg cgttcgagat gttttaattg
     1921 attgtagtgg aacggatgag attggttttt aacggcttta atggcgaccg ccgcaccgat
     1981 atcagaatga aaaaggaagc gatcgctccg gtaattgatt ttatcagcca atagaggcag
     2041 aaaaacgatt tgactgtcaa gaccctttgc cgcgtgaatc gtcataatcg ttaccgcgtc
     2101 acctttggcg gcggatagat tggcttcgcc ttcgcgcact tccttatctc gataaatttg
     2161 aatacgccgg ataaaatcaa ttggcgagag gctttcagct ttttcccatt ccagcgccaa
     2221 ttcaatcaat ttttttacgt tggcaatttt ctgggtgccg tccggtaacc cagccagaag
     2281 cgcggtaaaa ttagtcatct ccacaatttg attaattatt tcggcggtgg atagttgcgt
     2341 aatctgtttg tttaatt
//
LOCUS       DAOSMP010000142         2357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_45207_length_2357_cov_11.669852, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000142 DAOSMP010000000
VERSION     DAOSMP010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45207_length_2357_cov_11.669852"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..296)
                     /locus_tag="PKX09_10415"
                     /pseudo
     CDS             complement(87..296)
                     /locus_tag="PKX09_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011841288.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(664..1830)
                     /locus_tag="PKX09_10420"
     CDS             complement(664..1830)
                     /locus_tag="PKX09_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOU18273.1"
                     /translation="MDNFLKILQTTGFANIQIGNIIMIFIGLIAIYFAITKKYEPLLL
                     LPIGFGIIVGNIPSIPSIAIGVFDTVENGAASNSVFSMIYYLVTSGIFPPLIFLGIGA
                     LTDFSSLISNPKSLLLGAAAQLGIFVTFIGALILGFSAPEAASIGIIGGADGPTSIFL
                     ASQLAPHLLGPIAIAGYSYIALVPLIQPPVMKLLITEKEARIRMKPARKVSRKEKILF
                     PIIVTILAALIVPGSIPLVGMLMLGNLLKESGVTDRLAKTAGTAILDTCTILLSFTVG
                     ASTQAARYVDGVNVGFLTPRALSIFFLGFFSFIIATSGGLIFAKIMNFFLKEKINPLI
                     GAAGVSAVPDSARVVHMFAQRYDRDNHLLFHAMGPNVAGVIGSAIAAGVFLSIF"
     gene            complement(1834..2310)
                     /locus_tag="PKX09_10425"
     CDS             complement(1834..2310)
                     /locus_tag="PKX09_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOU18274.1"
                     /translation="MKEKKVELEINGQPYTVIINEISAYHASVTVNDRTYEVGLKDLG
                     FDQVADIKPKLINVPESAVPTAKPAGMTPPTQLHRPKSVIDSSAVVAPLPGLIQNILV
                     KVGDTVRAGQHVLTMEAMKMENEIQTNRDGIIKDIKVRIGDSVNEGDTLIILNSGE"
BASE COUNT          660 a          495 c          561 g          641 t
ORIGIN      
        1 gtgaccgaaa atctctacca aagacatatt cagccgttgt ttggggtgcc cgtggattaa
       61 attgtgaggg caaaaaatgt gtatgattaa ccttctggcg aaacattttt caaatattca
      121 ttaatgcgat caattgggcg tttgcccatg tttcggtaac cccgttggag tctttgttca
      181 ttgtattctc tcagccatat atccagatct ttttgtaaag cttcgactga ttcgtacatt
      241 ttagctcggg aagctggacg gaaaaattca tttaggacgg tacgattgaa tcgttctgtc
      301 ttggttcata aggaaatgga attttcgctc ctttcaaata aggataaatt tttacgattt
      361 cctgcccgcc ctcctatgtc gggagcaggc gggtcggcga cttgatctaa cttttttttg
      421 agtgggattt acttccaaaa gaatgaaggc tgggtcatgg gtgaaagggc aatatattat
      481 tcatccgatg gcggtgattc atggcaggaa caatatcggg tagacggagg cgaaaagctc
      541 gtaagcctct cctttgtaaa taattccaac ggatgggtat taagtgaaaa tggcaatgtt
      601 tatcggtatg gagaatagcg acgcaatttt ttaattattg gcttgtagtt gcgataatta
      661 ttactaaaat attgacagaa aaactccagc cgcgatagcc gagccgatca caccggcgac
      721 gtttgggccc atggcgtgga agagcaggtg attgtctcta tcataacgct gagcaaacat
      781 atgcacaacc cgcgctgaat cgggaactgc cgaaacgccc gccgcaccaa tcagcggatt
      841 gattttttct ttcaggaaaa agttcattat cttggcaaat atcaaaccgc ccgaagttgc
      901 gataataaaa gagaaaaatc cgaggaaaaa gatactcaat gcgcgggggg ttagaaaccc
      961 gacattgaca ccgtcaacgt aacgcgcggc ttgcgtgctg gcgcctaccg taaatgacag
     1021 taaaatagta caggtatcca gaatagctgt tccggcagtt ttcgccagcc ggtcagtgac
     1081 gccgctctct ttcagcaagt tgcccagcat caacataccc actaacggga tagaccccgg
     1141 tacgatcaga gcggctaaaa ttgtgacgat aatagggaag aggattttct ctttacggga
     1201 aactttgcga gccggtttca tacgaatgcg cgcctctttt tcggttatca atagtttcat
     1261 aaccggtggc tgaattaatg gaacgagtgc gatatacgaa taaccggcga ttgcgattgg
     1321 tcccagtaaa tggggcgcga gttgggaggc gagaaagatc gaagttggtc catctgctcc
     1381 tccaatgatg ccgatcgagg cggcttccgg cgcagaaaaa ccgagaatca acgcgccgat
     1441 aaaagtaacg aaaataccta actgagcggc ggctcccaat agaagtgatt tcggattaga
     1501 aattaatgat gaaaaatcgg ttaatgctcc gatgcccaga aaaatcagcg gtggaaatat
     1561 tcccgaagtc actaaataat aaatcatact gaaaacgctg ttactcgcag cgccattctc
     1621 aactgtgtcg aagaccccaa ttgcaataga tggtatggaa ggaatattgc ccacaattat
     1681 accgaaccca atcggcaaaa gcaggagcgg ttcgtacttt ttcgttatcg caaagtaaat
     1741 ggcaatcagc ccgataaaaa tcataataat attgccgatc tgaatattag caaagccagt
     1801 cgtttgtaaa attttcagga aattatccat ctatcactcc cctgaattga gaattatgag
     1861 ggtatcgcct tcgttgactg aatcgccgat tcggacttta atgtctttga taattccgtc
     1921 acgattagtc tggatttcgt tttccatttt catggcttcc attgtcagaa cgtgctgacc
     1981 ggcgcggacg gtatccccga ctttaacgag aatattttga attagacccg gcaagggcgc
     2041 taccacagcg gagctgtcga tcaccgattt aggccgatgc agttgagtag gcggagtcat
     2101 acccgccggt ttagcggttg gtacggctga ttcgggaaca ttaattaatt tgggtttaat
     2161 gtcggcaact tgatcgaagc ccaggtcttt taagccaact tcgtaagtgc gatcattgac
     2221 ggttacggaa gcgtggtagg cagaaatttc attgatgatg acagtgtaag gttgaccgtt
     2281 tatttctaat tctacttttt tctctttcat actatttcct caagaattga ctggcgattg
     2341 agccgatttt ccaattg
//
LOCUS       DAOSMP010000143         2333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_45817_length_2333_cov_16.843284, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000143 DAOSMP010000000
VERSION     DAOSMP010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45817_length_2333_cov_16.843284"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PKX09_10430"
     CDS             <1..792
                     /locus_tag="PKX09_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18275.1"
                     /translation="RVVSYFVGTAFLNAYEITGKSLYLNYAIGVKDFLVQAPKILFEN
                     DRMKCLSYVPDESINWVVMDVSALCGAFCARLAHFTNDKSLIEEARKLISYVVDKQTD
                     YGAWFYTHPPTANRLKMHDNYHTGYILDAILDYLDFSGDDSFLGNYHRGLQYYLENLF
                     LADGTPKWMNNKIYPIDVHGSAQGIITFLKAGRFNRQNEEFAYRIAEWAINNMQNKQE
                     GFFYYQKGRFFKKPFTIMHWSNGWMAIAMSEIIRKYYAENRATTN"
     gene            764..1528
                     /locus_tag="PKX09_10435"
     CDS             764..1528
                     /locus_tag="PKX09_10435"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOU18276.1"
                     /translation="MPKIEQQPTKKDVEEFWNRNVCQTEFLKTGERGTEEFYQEAERV
                     RYKYHFYLPELFDWIAKEKPNGSLLEVGCSMGTDLLQLARRGMRVTGIDLTEEGINLA
                     RKRFELYQIPAELKVDDAENLSFPDNTFDVVYSFGVLHHTPDTQKAIDEVWRVLVPGG
                     LAVIMLYNRKSLNYYIHRLINQPFDGNRKDRCPIERTYTKAQIFSMFQDFKKVKIEVE
                     YLLTTGYGIVWDLIPRIVHRTLGHLWGWHLVIKAYK"
     gene            1528..>2333
                     /locus_tag="PKX09_10440"
     CDS             1528..>2333
                     /locus_tag="PKX09_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18277.1"
                     /translation="MKKVLFISYYWPPAGGATTTRILKFYKYLPEFGWEPVILTVEDG
                     DFPFIDPQTQKEVRPDTKIYKAKNISLHRLFSKVAKNSKEMFVPFAFTEKNSKGIQGK
                     LSRWVKYNAIPDTRFLWKRFAIKKGLQIIRENDIKLIFSSSPPQTNHCIATELSRKTG
                     IPCVGDMRDPWTDVYWLQTNSQRWKWIHNLDQKIEKRTLNSMAAITTIGPSLVNLFQK
                     KVNQPVYLIYNGFEPLDIDLPPRKNAKWTITYAGSISIEQDIQAFCKAIEV"
BASE COUNT          730 a          429 c          499 g          675 t
ORIGIN      
        1 cgcgtggtgt cgtacttcgt cgggacggct ttcctaaatg cttatgaaat taccggaaag
       61 agtttgtatt taaattatgc gataggagta aaagattttt tagtccaagc tccaaagatc
      121 ttatttgaaa atgatagaat gaaatgctta agctatgtgc cagacgaaag cattaattgg
      181 gttgtaatgg atgtgagtgc tttatgtgga gcattttgtg cccggttagc ccattttacc
      241 aatgataaat ccttgatcga agaagcccgc aaattgatca gctacgtcgt tgataagcag
      301 accgactatg gcgcctggtt ttatactcac ccaccgactg ctaatcgttt aaagatgcac
      361 gacaattacc ataccggata tatattggat gcaattctgg attacttaga ttttagtgga
      421 gatgattcgt ttcttggaaa ttatcatcgc ggtctgcaat attatttaga gaatttgttc
      481 ttagccgatg gaaccccaaa atggatgaat aacaaaattt atcccattga cgtccacggg
      541 tcggctcagg ggatcattac ttttctaaaa gctggccggt ttaatcgcca aaatgaagaa
      601 tttgcttacc gaatcgccga gtgggctatc aataatatgc aaaataagca agaaggtttt
      661 ttttattacc aaaaaggaag attttttaaa aaaccattta ctattatgca ttggagtaat
      721 ggctggatgg caatagcaat gagtgaaata atcaggaaat attatgccga aaatagagca
      781 acaaccaact aaaaaagacg ttgaggaatt ctggaatcgg aatgtttgtc agactgaatt
      841 tctcaagact ggtgagcgcg ggacggaaga attttatcag gaagccgaac gtgttcgata
      901 taaatatcac ttctatctac ctgaattatt cgattggatc gctaaagaga aaccaaatgg
      961 ttcactcctt gaagtcggct gtagtatggg aactgattta ttgcaacttg ccaggcgcgg
     1021 aatgcgagta accgggattg acctgacgga agaaggaatt aatttagccc gtaaacgctt
     1081 tgaactatat cagataccag ctgagctaaa agtagatgat gcggaaaatc tctcctttcc
     1141 ggataatact tttgatgttg tctattcttt tggagtttta catcatactc cggataccca
     1201 aaaagccatt gatgaggtct ggcgggtatt ggttcccggt ggtttggcgg tgattatgct
     1261 gtataatcgt aaatcgctca attattacat tcatcgtctg attaatcagc cttttgacgg
     1321 taatcgtaaa gatcgctgtc cgattgaaag aacttatacc aaagctcaaa tattctcaat
     1381 gtttcaagat tttaaaaagg taaagatcga ggttgaatac ttgttaacca ccggatacgg
     1441 aatcgtttgg gatttgattc cccgaatagt ccatcggact cttgggcatt tatggggttg
     1501 gcatctggta ataaaagcat ataaataatg aaaaaagttc tgttcatttc atattactgg
     1561 ccaccggctg ggggtgcgac gacgacaaga atcctcaagt tttacaagta tctacctgaa
     1621 ttcggttggg agccagtcat tttaactgtc gaggatggcg atttcccatt tattgatcct
     1681 cagacacaga aagaagtcag accggacaca aaaatctata aggctaagaa tatctcttta
     1741 catcggcttt tctcaaaagt cgcgaagaat tccaaagaaa tgttcgttcc atttgctttt
     1801 acagagaaaa acagtaaagg tatacaagga aagttatcac gctgggtaaa atacaacgcg
     1861 attcccgata cgcggttctt atggaaaaga ttcgcgatta aaaaaggttt acagatcatt
     1921 agagaaaatg atattaagct aatcttttct tcatcgccac ctcagacgaa tcactgcatt
     1981 gctacggaat tatcacgaaa aacaggtatc ccgtgcgtcg gcgatatgcg agacccgtgg
     2041 acggatgtct attggttgca gaccaattcc caacgctgga aatggattca caatctggat
     2101 caaaagattg aaaaacggac actcaattca atggccgcta ttaccacaat cggtccatcg
     2161 ttagtcaatt tatttcaaaa gaaagttaat caaccggttt acctgatcta taatggtttt
     2221 gaacctcttg atattgactt gcccccgcga aaaaatgcaa aatggactat cacttatgct
     2281 ggaagcatca gtattgaaca ggatattcag gcattttgta aagccattga agt
//
LOCUS       DAOSMP010000144         2284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_47174_length_2284_cov_19.489009, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000144 DAOSMP010000000
VERSION     DAOSMP010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47174_length_2284_cov_19.489009"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PKX09_10445"
     CDS             <1..425
                     /locus_tag="PKX09_10445"
                     /inference="COORDINATES: protein motif:HMM:NF014065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HOU18278.1"
                     /translation="MTEMAKSIVRDGEGATKFVTVTINGANSLEDAKKMAFGIANSML
                     VKAALFGCDPNWGRILSAAGMVFAHFDPAVADLRINGVYLYRQGEPLNLNKTEIQRMM
                     TPVELEIVLDCNLGKDSAVVYTTDLSYDYIKINAQYHT"
     gene            434..1327
                     /gene="argB"
                     /locus_tag="PKX09_10450"
     CDS             434..1327
                     /gene="argB"
                     /locus_tag="PKX09_10450"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOU18279.1"
                     /translation="MQKIIDKAAVLIEALPFIKAFHGQKIVIKFGGSIMDSENAMKSV
                     LQDIVFMKYVGWLPVVIHGGGKAISEAMNNRGVKPQFVNGLRVTDRKTMEIVENVLAH
                     KINAQIVNLLNMMDGEAVGVSGLDNGLIRVDKMFTENNGQSVNLGFVGQVRYINANFL
                     NRLIESGKIPVIAPLGLGDDGESYNINADLVAGEIAASIGARKLVYLTDTPGILRTPG
                     DEKSIISTINRQEAEKLIAAGIIEGGMIPKVNSCLDALRFGVNKGHIIDGRIKHSLLL
                     EIFTKEGIGTEILKDGSERIR"
     gene            1305..>2284
                     /locus_tag="PKX09_10455"
     CDS             1305..>2284
                     /locus_tag="PKX09_10455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HOU18280.1"
                     /translation="MDRKEFDNYVLPTYKRFPITLVKGRGMQVWDDEGNEYLDFFPGW
                     GSGNIGHCHPKVVAALHEQLDKIMLVPNVYYNEKAGELAKLIIQDSFDGQVFFGNSGA
                     EANEGALKLVRKRNPNKKTIITLNNSFHGRTLATVTATGQSEYQKGFDPLLGGFVYCD
                     INDYAMLASLMNENVAAIMLEVVQGEGGITPVTPKFIQTARELCDRFDAMLIIDEVQT
                     GIGRTGKMFAYQYFDVQPDVMTLAKSLGGGMPIGAFAVARQYCNILQPGSHGSTFGGN
                     PLACAAGVAVFQAIHEENLLENAVEMGTYFRQKLEELKSQAPVIREIRGLG"
BASE COUNT          634 a          522 c          557 g          571 t
ORIGIN      
        1 tgatgaccga aatggcaaaa tccattgtcc gagacggcga aggcgccacc aagttcgtga
       61 ccgttactat caatggcgcc aatagtttag aagatgccaa aaaaatggca ttcggcattg
      121 ctaattccat gttggtcaaa gccgcgcttt ttggatgcga cccgaattgg ggcagaattc
      181 tctccgcggc gggaatggta tttgcccatt ttgatccggc tgtcgctgat ttgcgcatca
      241 atggagttta cctctaccgg cagggtgaac cgcttaattt gaataaaacc gaaattcaac
      301 gtatgatgac accggtcgaa ctggagattg tactcgactg caacctcgga aaagatagcg
      361 ccgtagttta cactaccgac ctttcctatg actatatcaa aattaacgct caatatcata
      421 cttaggagct gttatgcaga aaattattga caaagccgcc gttttgatag aagctctgcc
      481 tttcatcaaa gcctttcacg gccaaaaaat cgtgatcaaa ttcggtggca gtataatgga
      541 cagtgaaaat gctatgaaat ctgttttgca ggacattgtc tttatgaaat acgtcggctg
      601 gttgccggtg gtcatccacg gcggcggcaa agccattagc gaggcaatga ataaccgtgg
      661 agttaaacca caattcgtta atggactgcg cgtaaccgac cgcaaaacta tggaaatcgt
      721 tgaaaatgtt ctcgcccaca aaatcaatgc tcaaattgtt aacttgctga atatgatgga
      781 cggcgaggcg gttggagttt ccggactcga taacggatta atacgcgttg ataaaatgtt
      841 caccgaaaac aatgggcaat cggtaaacct cggttttgtt ggtcaggttc gctacattaa
      901 tgctaatttt cttaatcggc ttatagaaag cggaaaaatt cctgtaattg caccactcgg
      961 tttgggtgat gatggcgaaa gctacaatat caatgccgac cttgttgctg gcgaaatcgc
     1021 ggcttcgatt ggggctcgca agctagttta tttaactgat acccctggca ttttgcggac
     1081 acccggcgac gagaaaagta tcattagtac cattaatcgc caggaggctg aaaaacttat
     1141 cgccgcgggc atcatcgagg gcggtatgat tcccaaagtc aactcctgtc tcgatgcttt
     1201 gaggtttggg gtcaacaaag gtcacattat agacgggcgc attaagcact cgctcttact
     1261 cgaaattttc actaaagaag gaataggaac ggagatatta aaagatggat cggaaagaat
     1321 tcgataatta cgttttacct acttataagc gtttcccgat taccctggta aaaggacgcg
     1381 gtatgcaggt ctgggatgat gaaggcaacg agtacctcga ctttttcccc ggttggggta
     1441 gtggcaatat cggacattgc catcccaaag tcgtggcggc tctgcatgaa caattagaca
     1501 aaatcatgct cgtaccgaat gtctattata acgagaaagc cggcgaactt gccaaactaa
     1561 tcattcagga cagctttgat ggacaagttt tcttcggcaa tagcggcgcg gaagccaatg
     1621 aaggagcact gaagctggtg cgcaaacgca accccaataa gaaaacgatt attacgctga
     1681 ataactcctt tcatggacgc acgctggcta cagttaccgc aaccggtcag agcgaatacc
     1741 agaaaggctt tgatcccctg ctcggcggtt ttgtctattg cgatatcaat gattacgcta
     1801 tgctcgcaag cttaatgaac gaaaacgtcg cggctattat gctcgaagtc gtccagggcg
     1861 aaggcggcat tacacctgtc acaccaaaat ttattcagac cgcgcgcgaa ctgtgcgacc
     1921 ggtttgacgc tatgctaatc atagatgaag ttcagaccgg catcgggcgc accggtaaaa
     1981 tgtttgccta ccaatatttc gatgtccaac cggacgttat gactctcgcc aaatcactcg
     2041 gcggcggtat gccgatcggc gcttttgccg tagcacgcca atattgcaat attttacaac
     2101 ccggcagtca cggctcgact ttcggtggta atccactggc ttgcgccgct ggtgtggcgg
     2161 tttttcaagc tattcacgag gaaaatctac tcgagaatgc cgttgaaatg ggcacctatt
     2221 tccggcaaaa attggaagaa ctgaaaagcc aagcgccggt aattcgtgaa atccgcgggc
     2281 tcgg
//
LOCUS       DAOSMP010000145         2277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_47353_length_2277_cov_14.252025, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000145 DAOSMP010000000
VERSION     DAOSMP010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47353_length_2277_cov_14.252025"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PKX09_10460"
     CDS             <1..990
                     /locus_tag="PKX09_10460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU18281.1"
                     /translation="FSGGNLGGWDLIPGDMEGDVEISGTAAPTGWVALRGGFDRYELN
                     ETYALKISGQIELVGGGFQDAGSLRYGIFYSDSAGTTWQDSTLDSNWVWKGTDNYHSG
                     YLITPPSGSNVATWSNGSGTWGSVTNGKWWDISAPSAHVLGNIAQDPVGGVAGAGIYD
                     FTISISKGTSGNLLVFTLSKKDGTYYYAHSTTTPVASVTDEINSIGFAINNSTTTALK
                     LYKVKVDRGDHFNGLEEAIAAQLPKAYALKQNYPNPFNPTTNIRFDLPKDSDVNLVVY
                     DLMGREVAKLVNGPMNAGYYTINFNAANLPSGVYIYRLKAGDFVSTKKLMLLK"
     gene            1166..>2277
                     /locus_tag="PKX09_10465"
     CDS             1166..>2277
                     /locus_tag="PKX09_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HOU18282.1"
                     /translation="MSGDLIPAQNLRSDNNTQPRQRISLNDGWKFYKYESTTNADDLI
                     YDVRPEISDYNEVKPADAQPTEGEKLKTIRTVLKPWVLSTGNDFIKDSTKHHHRPEGN
                     PGCVFPFVQKDYDDSQWEIVNLPHDWAIKGPFFDGSNPVVGGGMGRLPSPGVVWYRKK
                     LDIPATDAGKSIFLDVDGAMSYAMVWLNGYLVGGWPYGYSSWRLDLTPFIIPGAENQL
                     AIRLDNPNHSSRWYPGGGIYRNVWLVKTNPIHIAQWGTYITAENISATCATLNLEITI
                     DNDSRNDANVKAITEVFVLNENGDKIGKPVTYFKPMEIIIFTGTRTSEKNSIMIKNPK
                     LWGPPPTQTPNCYVAVTTLWQDDKPIDQYETRFGIRS"
BASE COUNT          635 a          463 c          535 g          644 t
ORIGIN      
        1 ttcagtggag gtaatcttgg tggctgggac cttattcctg gagacatgga aggcgatgtt
       61 gaaatctcag gaactgccgc accaactgga tgggttgcct tacgcggtgg ttttgatcgg
      121 tatgagctga atgaaactta tgctctgaaa atatcaggac aaattgaatt agtaggcggt
      181 ggatttcagg atgcaggtag tttgcgctat ggaatattct atagtgacag tgctggtaca
      241 acctggcaag actcaacctt ggattccaac tgggtctgga aaggaacgga taattatcac
      301 tccggttatc taatcacacc tccaagtggt tctaatgtag cgacctggtc gaatggttca
      361 ggaacctggg gttctgtgac aaatggtaag tggtgggata ttagtgctcc cagtgctcat
      421 gttttgggta atatagcaca agatccggtt ggcggagttg cgggcgccgg tatctatgat
      481 tttaccatct cgatttcaaa aggaacaagc ggtaatctgc ttgtatttac tttatctaaa
      541 aaagatggga cctattatta tgcccatagc acgaccactc cagtagcatc tgtaacggat
      601 gagattaata gtattggttt cgctatcaac aattcgacta ctacggctct aaagttatat
      661 aaagttaaag ttgatagagg tgatcatttc aatgggctag aagaagcaat tgcagcccaa
      721 ttgcccaagg cttacgcttt gaagcagaac tatccgaacc cgtttaaccc gacgactaat
      781 attcggtttg atttaccgaa agacagtgat gtcaatttgg tcgtctatga tttaatgggt
      841 cgtgaagtag ccaagctggt caatggtcct atgaatgccg gctattatac gattaatttc
      901 aacgcggcga atctgccatc cggagtttat atctatagac tcaaggctgg tgattttgtc
      961 agcaccaaga aacttatgct tctgaaatga gctgttagtt ctttggtttt ttgaatgtgg
     1021 aatcagcctg cccgtctcag cgggcaggct gattttgtta gtggagtttt tgattgatta
     1081 aaacctattg ctattgataa tgataagtaa cgctaaattt ttatttaaaa ccgtaatctt
     1141 aagtataata gtatgcataa ttatattgag tggtgatcta attcctgccc aaaatttaag
     1201 gtcagacaat aacactcagc cgcgccagcg catttctcta aatgatggtt ggaaatttta
     1261 taagtatgaa tctaccacaa atgccgacga cctaatctac gatgtgcgac cagaaattag
     1321 cgattataat gaggtcaaac cggctgatgc ccaaccgacc gaaggcgaaa agttaaagac
     1381 aatccgaacg gtgttgaaac cctgggtttt atcaacaggc aatgatttta ttaaggattc
     1441 tacgaaacat caccatcgcc ctgaaggtaa tccgggctgt gtttttcctt tcgttcagaa
     1501 agattacgat gatagtcagt gggagattgt gaatttgccg cacgactggg cgattaaggg
     1561 accgtttttt gatggctcga atccggtagt cggcggaggt atgggacgac ttcctagccc
     1621 cggagtagtc tggtatcgga aaaaattgga cattccagct accgacgctg gcaaatccat
     1681 atttctcgat gtggacggcg caatgtccta cgctatggtc tggttgaacg gttatcttgt
     1741 cggcggctgg ccttacggat attcttcctg gcgtctcgac ctgacgcctt ttatcatacc
     1801 gggtgctgag aatcaactag cgattcgtct tgacaatccc aatcattcgt cgcgctggta
     1861 ccccggcggt ggaatctacc gcaatgtctg gctggtcaaa accaatccga tccatatcgc
     1921 ccaatggggt acttatatta cggctgaaaa tatttcggcg acctgcgcga cgctcaacct
     1981 ggaaattaca atagacaatg attcccggaa cgacgcaaat gtaaaagcga ttaccgaagt
     2041 ttttgtctta aatgaaaatg gtgataaaat cggtaagcca gtcacctatt tcaaaccaat
     2101 ggaaattata attttcaccg gtactcgaac cagtgaaaaa aattcaatta tgatcaaaaa
     2161 tccaaaacta tggggaccgc cgcctactca gacacctaat tgctacgttg cggttactac
     2221 tctatggcag gatgacaaac cgattgatca gtacgaaacg cgttttggta ttcgttc
//
LOCUS       DAOSMP010000146         2276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_47390_length_2276_cov_17.110311, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000146 DAOSMP010000000
VERSION     DAOSMP010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47390_length_2276_cov_17.110311"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..832)
                     /locus_tag="PKX09_10470"
     CDS             complement(44..832)
                     /locus_tag="PKX09_10470"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HOU18283.1"
                     /translation="MKELVSLAESAARAAGAILRSYFQQEYTITHKGLDDPVTNADTE
                     ADAFLKEKLLSACPDYGWLSEESADSAERLTKSRVWIVDPLDGTKEFIAGRPEFMVSI
                     GLAENGQPVLGVLYNPINDELFSAYKGQGAFLNGSRISCTQTKTLSEARLIVSRSELI
                     ENLWQPYRHFFKTLQPCGSVAYKLALVASGKADIHISLKPKNEWDICAAHCLLAEAGA
                     VLIGRDGMPIFYNLKYPIIPTGVIAGNKKLVVAGQKITQLKVCN"
     gene            complement(854..2131)
                     /locus_tag="PKX09_10475"
     CDS             complement(854..2131)
                     /locus_tag="PKX09_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L-dopachrome tautomerase-related protein"
                     /protein_id="HOU18284.1"
                     /translation="MFKKGLILLILASAVFAQSNPPAIRSDSVGIYFNMSIQAYSEQQ
                     YDRYLEYTQKLIQVFPDDFTLQYNLASAYALNKDTVNAIKILNSLVDKGLGLVAEDDP
                     DFADLKNTPEFRALVKKIEKVKKPVKSSKKAFTVAEKDLIPRGITYDSQTKNLYISSL
                     YKSKIVQIDRNGKISDFVPERQDGLGPVTAIQADPQRRILWALSSFGIPNEKTPRELA
                     GTASVYKYNLNDGKLLKKYNLPRPNRHFLTDLTVDRAGNLYIADSGQRTIFRLDAQKD
                     TLEKFLELYKYPQLASLTITPDDKHLFVTYGGGILNIDVATGKITPLTSPDHILLVGC
                     DGLNFYKNSLIAIQTFLNRIVRFELSPDFSSVTDYKIIESNNPDFNIPSGGAIDGKYF
                     FYVANSQINSLDRSGNVMTPEQLKDILIFKAKL"
BASE COUNT          608 a          489 c          531 g          648 t
ORIGIN      
        1 gatgtctatc ataattaacc ggcaaatata gaacaactgg aggttaattg caaaccttaa
       61 gctgggtaat tttttgtcct gccacaacga gctttttatt cccggcaatt accccagttg
      121 ggataatcgg gtacttaaga ttgtagaata ttggcattcc atcgcgaccg atcaaaacgg
      181 caccggcttc agccagtagg caatgggcgg cgcagatgtc ccattcattt ttcggtttca
      241 ggctgatatg gatatcggct ttcccgctgg cgaccagtgc caatttataa gcgacactac
      301 cacacggttg cagggttttg aaaaaatgac gatagggttg ccagagattt tcaattagct
      361 cggagcggct gacgatcaaa cgcgcctcac tgagagtttt ggtctgagta caagaaatcc
      421 gactaccgtt caggaaagcg ccttgtcctt tatatgccga gaaaagttcg tcgttaattg
      481 gattatataa aactcccaaa accggttgcc cattttcagc taatccgatt gaaaccataa
      541 attccggtcg cccggcaatg aattcctttg tgccgtccag cggatcaacg atccaaactc
      601 gcgatttcgt caagcgttcg gcggaatcag cgctttcctc agacaaccag ccgtaatccg
      661 gacaagcgct aagtaatttt tctttcagga aggcatcggc ttcagtatca gcatttgtga
      721 ccggatcgtc aagacctttg tgggtaattg tatattcctg ctgaaagtaa ctccgcagta
      781 tagcaccggc ggctcgggcg gctgattcag ccaaactgac aagttccttc atagcgaata
      841 aaggatctgc cagctacagt ttggctttga aaatcaggat gtccttcaac tgctcgggtg
      901 tcatcacgtt gcccgaacgg tcgaggctat tgatttggct gttagcaacg tagaaaaaat
      961 acttgccgtc aattgctcct ccggatggaa tgttgaaatc gggattattg gattcgatga
     1021 ttttgtaatc ggtgacactg ctaaaatccg gacttaattc gaaacgcacg atgcgattca
     1081 agaaggtttg aatggctatt aaggagttct tataaaaatt caagccgtca caacctacca
     1141 gcaaaatatg gtccggactg gttagcggtg tgattttgcc ggtggcgaca tcaatgttca
     1201 gaataccgcc gccatacgtt acgaatagat gtttatcgtc aggcgtaatg gtcaagctag
     1261 ccaattgcgg atatttatac aattcgagga atttttcgag agtatctttt tgggcatcga
     1321 gacggaaaat agtccgctga ccggaatcgg caatataaag attgccagcc cggtctactg
     1381 tcaaatcagt cagaaaatgc cgattaggcc tgggcaaatt gtatttctta aggagtttgc
     1441 cgtcatttag gttgtatttg taaacgctgg cggttccagc caattcccgg ggcgtttttt
     1501 cattggggat tccaaatgaa cttagcgccc acaaaatccg acgctgggga tcagcctgaa
     1561 tggcggttac cggaccaagt ccatcctgcc gttcaggaac aaaatcgcta attttaccat
     1621 tgcggtcaat ttggacaatt ttcgatttat aaagactgct tatatatagg tttttagttt
     1681 gtgagtcgta ggtgatgccg cgcggaatga ggtctttttc ggcaacggta aaggcttttt
     1741 tactcgattt taccggcttt ttaacctttt caattttctt gaccagagcg cggaattccg
     1801 gtgtattttt caaatcggca aaatcgggat cgtcttctgc taccaaccct aagcctttat
     1861 ctactaatga attcaggatt ttgatagcat tgacagtgtc cttatttaaa gcataggcac
     1921 tggcgagatt atactgtagg gtaaaatcgt ctggaaaaac ctgaataagt ttctgagtat
     1981 attcaagata gcgatcatat tgctgttcac tgtaagcttg aatactcata ttgaagtaaa
     2041 tcccaacgct atccgatcga atcgcgggcg gattggattg ggcaaaaact gcggaagcca
     2101 ggattaacaa gattaaacct tttttaaaca taattaacta ctttccttta tttaaaataa
     2161 ctacttggca attggcaagt tgggcaaatt tttcggcatc agtccgacta ccggagacgg
     2221 taccccagtg catcggtacg gcgacctgcg ggtggattat tttggtggct tgggcg
//
LOCUS       DAOSMP010000147         2243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_48319_length_2243_cov_10.946527, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000147 DAOSMP010000000
VERSION     DAOSMP010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48319_length_2243_cov_10.946527"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1548
                     /locus_tag="PKX09_10480"
     CDS             190..1548
                     /locus_tag="PKX09_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOU18285.1"
                     /translation="MNSVQRHNRHKCKIITVFWLIVILPVLIQCEPNNKSDQIGGSDL
                     TFVEEHGNLTVSGNRIIDQNGDPIVLRGMSLFWSQWMGKYYNYNCIKWLRDDWKCTVV
                     RAAMGIESGGYLTNPATEKAKIVTVIEACIDLGIYVIVDWHDHNAHEHQAQAIDFFQE
                     IATGYGTYPNVIYEVFNEPEQDNWKTVLKPYLNAVVDSIRAIDPDNLIVVGTPNWSQD
                     VDIASQNPLTQNNIAYALHFYAAYQYHKQTLRNKASTALKNGIALFVTEFGTVLNTGD
                     GPIDTAETRLWMNFMESNQISWCNWSIADKNETSAALKPGASANGGWSASEISESGLL
                     IRDYIIRGNTKQPNLVRKTRLIPAGFALAQNYPNPFNSSTQISFELPVSAGVNLGVYD
                     LRGNLICTLLNKVFPAGCYQTTWETNGAIGEIAGGVYILRFLADSEIGTTSISRKLSL
                     IK"
     gene            1592..>2243
                     /locus_tag="PKX09_10485"
     CDS             1592..>2243
                     /locus_tag="PKX09_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOU18286.1"
                     /translation="MRKTYLGGFLFFLILLNLFTNNNRLFSQEFNRDMETFQPLSYAE
                     ADKLAESILAQMTLDEKIALIGGDKYFFIRALPRLNLSEIYMSDATQGIHIRQSFNEI
                     DLSDYQPEKSTAFPCPISLAATWNRELAYQYARAIGEECKAANIGILLGPGMNIYRQS
                     QCGRNFEYFGEDPYLTARIIENYVQGVQSTGTVATLKHFLANNTEYYRKKSNSVVDE"
BASE COUNT          683 a          452 c          474 g          634 t
ORIGIN      
        1 tccctattcc ggacacggtt tgccggcaga aaattaccag gcgcggatga attggattcg
       61 ccagcaactg aatcggaaat aatcaataaa tagcactatc gttgtcaata taaaggtaat
      121 tttccggttg tatataaata gggatgattg atggatgcaa aactcaatcg ctaaatggaa
      181 tttttataaa tgaattcagt tcagagacat aatcgtcata agtgtaaaat tattacagtg
      241 ttttggttaa tagttatatt accagttctt atccaatgtg agccgaataa taaatccgat
      301 caaatcggtg gttccgattt aaccttcgta gaagagcatg gaaatcttac ggtcagcggt
      361 aaccgcatta ttgatcaaaa tggtgatccg atagtgttgc gcggaatgag tctcttctgg
      421 agccaatgga tgggaaaata ctacaattac aattgtatta aatggcttcg agatgactgg
      481 aaatgcaccg ttgtccgggc ggcaatgggt atcgaatcgg gtggttattt gaccaatccc
      541 gcaactgaaa aggctaaaat tgtgaccgtc atcgaggctt gtattgattt aggaatttat
      601 gtcattgtgg attggcatga tcataatgct catgaacacc aggcgcaggc gattgatttc
      661 tttcaagaaa ttgctactgg ttacggtacc tatcccaacg tgatttatga agtatttaac
      721 gaacctgaac aggacaattg gaagaccgta ctaaagcctt atttgaacgc cgtagttgat
      781 tcaataaggg cgattgatcc cgacaattta atcgtggtcg gaacgccaaa ctggtcgcag
      841 gatgtagata ttgcctcgca gaatccgctc acacagaaca atattgccta tgctttgcat
      901 ttttatgcgg cttaccagta tcataaacaa accttacgca ataaggcatc aactgcatta
      961 aaaaatggga tcgcgctttt tgtgacagaa tttggtactg ttctgaatac cggcgacggt
     1021 ccaattgata ccgccgaaac ccgcctctgg atgaatttta tggagagtaa tcaaatcagc
     1081 tggtgcaatt ggtcaattgc cgataaaaat gaaacctcag ccgccttaaa accgggcgcc
     1141 tcggctaacg gcggctggtc ggcatccgaa atttccgaat caggattgtt aattagggat
     1201 tatatcatca gaggtaatac caaacaaccc aatctggtta gaaaaacacg acttattcca
     1261 gccggatttg ccttagctca aaattatcca aatcccttta attcttctac ccagataagc
     1321 ttcgaattac cagttagcgc cggggttaat cttggtgttt acgatttacg cggtaatttg
     1381 atctgcactt tgctaaataa agtgtttccc gccggatgct accaaaccac ctgggaaacg
     1441 aacggtgcga ttggcgaaat tgccggtgga gtatatatat taagattttt agccgattcc
     1501 gaaattggaa cgacttcaat ttcccgaaaa ttatcactta taaaatgaga ctaagagaat
     1561 aaattgaacc aaattaatta caggagttat aatgcgcaaa acctacttag gtggtttttt
     1621 attttttcta attttactaa acctgttcac caacaataat cgtttattca gtcaggaatt
     1681 caatcgagat atggaaacgt ttcagcctct atcctatgca gaagcagata aattagccga
     1741 atcgatcttg gctcaaatga ccttagatga gaaaatcgct ttgattggcg gcgataaata
     1801 cttctttatt cgagcgctac cgcgtttgaa tctttcggaa atttatatgt cggatgcgac
     1861 tcaaggcatc catatccgtc agagctttaa tgaaattgac ctctcggatt accagccgga
     1921 aaaatcaaca gcctttccct gtccaatttc attagccgcc acctggaacc gtgagttagc
     1981 ttatcaatac gcccgagcga tcggtgagga atgtaaagcc gccaatattg gtattttact
     2041 cggaccgggg atgaacattt atcgccaatc ccaatgtggt cgcaattttg agtattttgg
     2101 cgaagatccc tacttaacag ctcggataat agagaattat gttcagggag tccagagtac
     2161 cggaaccgta gccaccctaa aacatttttt agccaataat accgaatact atcggaagaa
     2221 aagcaactcg gtagttgatg aac
//
LOCUS       DAOSMP010000148         2225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_48841_length_2225_cov_9.967281, whole genome shotgun sequence.
ACCESSION   DAOSMP010000148 DAOSMP010000000
VERSION     DAOSMP010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48841_length_2225_cov_9.967281"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2108)
                     /locus_tag="PKX09_10490"
     CDS             complement(<1..2108)
                     /locus_tag="PKX09_10490"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOU18287.1"
                     /translation="MTYTSIKLTWLDNDGDYPAEQFLVVLETASSFAPVDGQVYSDIG
                     ENKWVKYVNHGVQTVTFTGLDDGVTYYAKIWPVSNTSVGGEYYDYKTDGIVPELSQAT
                     QVYPSLPFVENFDYEIGELLTNHGWVAHSGSGSQTIDVTSGLVFDGYLSSGIGGAANL
                     DNTGEDVHSVFDPVTSGTVYASFIIQTASPNAAGYFFHLGQSTIGTTWLGRVWVNATG
                     DGVAISSGTAPSNYVAITPGKPTLVVVKYDLATKVSSLYVFDTFPTAEPAEPNYTFTE
                     TSTFDNVGSVALRQYNASQRVIVDGIRVDTTWAGAVAPAPLDLVSTPTFDPPAGNYYT
                     AQNVTISTTTEGATIYYTTDGSDPTESSTVYTNPIPVSSTTTIKAKAYKTGYTPSSVA
                     TAVYSFPIEVANIAALRASPVGSTWYKLIGESILTFKSSTRNAKYIQDTTGAILIDDY
                     SGIITTSYNLYDGITGIIGSLQDYNGMLQFVPVTDPGPATSTGNIVVPVEVTLAELNT
                     DYQGKLVKVKNVVISATGNFEVSKTYTISDITGSGVIRTQYADLDYLGKPIPTTLQNL
                     TGVVLQYQTTTQLIPRSLTDFEDASLTQYRSKISGNWTDAGTWQKLVGSEWLDATNAP
                     EGFSDYITIKASDTVTVNDTLRIGSGATVKVDGYLKNIASIISNGTFVFSNGSIYEHA
                     IDKDAIPIATWEEGATCLITG"
BASE COUNT          637 a          502 c          461 g          625 t
ORIGIN      
        1 ccggttatca aacaggtcgc cccttcttcc caggttgcaa taggaatggc atctttatca
       61 atggcatgtt catagatact tccattggaa aaaacaaatg tgccgttact gatgatactt
      121 gcgatattct ttaaataacc atcaactttt acagttgcac cagaacctat tcgaagagta
      181 tcatttacag taacagtatc actagcttta atagtaatat aatcggaaaa gccttccggc
      241 gcgttggtgg cgtctaacca ttcagatcca accaatttct gccaggtgcc ggcgtcggtc
      301 cagttgccac taattttgct acggtactgc gtcaacgatg cgtcttcgaa atcggtgaga
      361 ctgcgtggga ttagctgagt cgttgtctga tattgcagaa ctactcccgt caaattctgg
      421 agagtagtag gaattggttt acctaaataa tccaaatcgg catattgggt acgtataaca
      481 cccgatccag ttatatcgga aatcgtataa gttttactta cttcaaaatt accagttgct
      541 gatattacta cattttttac cttaaccaat ttaccttgat agtcggtatt cagttctgcg
      601 agagtaacct cgactggtac aacaatgttc cctgtactgg tggctgggcc gggatcggtc
      661 actggcacga attgtaacat tccattataa tcctgcaaag aaccgataat tccagtgatt
      721 ccatcatata agttatatga agtagtgatt atgccagaat agtcgtctat taaaatagca
      781 ccagtagtat cctgaatata tttagcattc cgggtactag atttaaaagt caaaatggat
      841 tctccaatga gtttatacca ggttgaccca actggtgaag ctcttaaagc cgcaatattt
      901 gccacttcga ttggaaagga ataaacggct gtggcaaccg aacttggtgt atacccggtt
      961 ttatatgcct tagcttttat ggtcgtagta ctactaaccg gaatcgggtt cgtataaact
     1021 gtactacttt cagttggatc actgccatca gtagtataat aaatagtcgc tccttcagta
     1081 gtagttgaaa tagttacgtt ttgagcggta taataattac cggctggtgg atcaaaagtc
     1141 ggtgtgctga ccagatcgag cggtgcggga gcaactgccc ctgcccaggt cgtatccacc
     1201 cgaatgccat caacaataac ccgctgagaa gcattatatt gtcgcagggc aacactgccg
     1261 acattgtcaa aagtactggt ttcagtaaag gtataattgg gttcagccgg ttctgcggtt
     1321 gggaatgtat caaaaacgta cagactgctt actttcgtag ccaaatcata tttcacaact
     1381 acaagtgtcg gtttacccgg tgtgatagca acataattag atggcgcagt accactactg
     1441 atcgctacac catcaccagt ggcgttcacc catactcttc ctaaccatgt cgtaccaatt
     1501 gttgactgac ctaagtggaa gaaatacccc gccgcattcg ggcttgcggt ttggataata
     1561 aatgaggcat agactgttcc cgaagtaacc ggatcaaaaa cgctgtgaac atcttctcca
     1621 gtattatcaa gattagccgc tccgccaata ccagaactta ggtagccgtc aaataccaat
     1681 ccgctggtca catcaatagt ttgacttcca ctaccactat gagcaaccca accgtgatta
     1741 gtgaggagct cacctatttc ataatcaaaa ttttctacga acggtaggct cgggtagact
     1801 tgagtcgctt gagaaagctc gggaactatt ccatctgttt tgtagtcata gtattcacca
     1861 ccaacactag tattggaaac cggccagatt ttagcgtaat aagtaacacc gtcatccaga
     1921 ccagtgaaag tcacggtttg gacaccgtga ttaacatatt tcacccattt attttcacca
     1981 atatcagaat atacttgtcc atctaccggg gcaaaactac tggctgtttc cagaacgacc
     2041 agaaattgtt cggccggata atcaccatcg ttatccaacc aggtcagctt gatactggta
     2101 taggtcactt cggttgccgt aaaagaggcg acatggttgg aaggctccgc catcaaagtc
     2161 ttggcgctac tactaacacc cgtggagtaa ttcaggcttc catctacgga aaagaattta
     2221 taata
//
LOCUS       DAOSMP010000149         2189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_49972_length_2189_cov_14.942362, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000149 DAOSMP010000000
VERSION     DAOSMP010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49972_length_2189_cov_14.942362"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..881
                     /gene="speB"
                     /locus_tag="PKX09_10495"
     CDS             6..881
                     /gene="speB"
                     /locus_tag="PKX09_10495"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOU18288.1"
                     /translation="MPTPFSPGAFGALEPDYCRYEQSRIAILPIPYDGTATWQKGAAK
                     GPEALLAASANMELYDIETDSEIYRQGITTLPFLKCPKKPEDLVQKVNEKTRQLLADG
                     KFVVGIGGEHSISIGLVQAHAKHFSNLSVLQLDAHSDLRNEYERSKYNHACVMARIQE
                     ICPIMQVGIRSMDISELERLDKARVIFAHEFCNSPEFFAKITANLTNNVYLTIDLDVF
                     DPAVFPSTGTPEPGGLGWYDVINVIAHIIQKKHIIGMDVNELLPNPSDIAPDFLAAKL
                     IYRTLSMIYTKESME"
     gene            878..1897
                     /locus_tag="PKX09_10500"
     CDS             878..1897
                     /locus_tag="PKX09_10500"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HOU18289.1"
                     /translation="MKKNDYLQKVVEHIDIKSFDARPILKAYSKMAFQARNLAVAADI
                     YDRMLKDEKCKVILTLAGSLFSAGLKNVVADMVENNMVDAIVSTGALIVDQDFFEALG
                     YKHYIGTPFSNDNELRDLAIDRIYDTYIDEDELRVCDLTIAEIANSLPPRPFSSREFI
                     EEMGKYLDKVGIKTEKSVVYSAYKKGVPIFVPAFSDCSAGFGLIHHQWNRKGNMVTID
                     SARDFLELTKIKIAAKETGILMIGGGVPKNFTQDVVVATDVLGIEKPMHKYAVQLTVA
                     DERDGGLSGSTLKEASSWGKVDTKYEQMVFGEATVMMPLLVSYAYHQGNWKKRQEARF
                     SNLFV"
     gene            complement(2008..>2189)
                     /locus_tag="PKX09_10505"
     CDS             complement(2008..>2189)
                     /locus_tag="PKX09_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18290.1"
                     /translation="VNTILIFWNPTVLVLNNQPWKVVDVVGAILAGVFIIVFIIVGIK
                     DAIKLDRLDRAKWEK"
BASE COUNT          631 a          480 c          476 g          602 t
ORIGIN      
        1 aaactatgcc gactcccttt tcacctggag catttggtgc gctcgagccg gattattgcc
       61 gatacgagca atcccggata gccattctac caattcctta cgacggaaca gccacttggc
      121 aaaaaggggc cgctaaaggt cctgaagcat tattggccgc ctcggctaat atggaacttt
      181 acgatattga gaccgattcg gagatttatc ggcaaggcat cactacgcta cccttcctca
      241 aatgccccaa aaagccggag gatttagtcc aaaaagtcaa cgaaaaaacc aggcagcttc
      301 tcgccgatgg aaaatttgta gtcggtatcg gcggtgaaca ctcgatttcg atcggactgg
      361 tacaggctca cgccaagcac ttctctaact tgtcagtctt gcagctggat gcccactccg
      421 atttgcggaa cgaatatgag cgtagtaaat acaatcacgc ttgcgtaatg gcacgtattc
      481 aagaaatctg cccaatcatg caggtcggca ttcgtagtat ggacattagc gaattagaac
      541 gattagataa agcgcgggtg atttttgcgc atgaattctg caattcgccg gaatttttcg
      601 ctaaaatcac cgccaattta accaacaatg tttatctgac tatcgatctg gatgttttcg
      661 atccggcggt tttcccctct accggtactc ctgaaccggg tggactgggc tggtacgatg
      721 ttatcaacgt cattgcgcat attattcaaa aaaagcacat catcggtatg gacgttaatg
      781 agcttttgcc taacccatct gacatagccc ctgattttct agcggcaaaa ctcatttatc
      841 gaacattaag tatgatttat acaaaggagt caatggaatg aaaaaaaacg actatctgca
      901 aaaggtggtt gaacatattg atattaaatc attcgacgcg cgaccgattc tgaaagctta
      961 tagtaaaatg gcttttcagg cgcgcaattt agctgtcgcc gccgatattt atgaccgaat
     1021 gctgaaagac gaaaaatgta aagtcatcct tactctagcc gggtcactat tcagtgccgg
     1081 attgaaaaac gtcgtagctg atatggtcga aaataatatg gtagatgcta tcgtctcaac
     1141 tggggctttg atcgttgatc aggatttttt tgaagcgctt ggttataaac attatattgg
     1201 aacccctttt agtaatgata acgaattgcg cgatctggcg attgaccgca tatatgacac
     1261 ttatatcgat gaagatgaac tgcgcgtctg cgatctgacc atcgccgaaa tcgctaattc
     1321 tctgccgccg cggccttttt cttcgcgcga gtttatcgag gaaatgggaa agtatctcga
     1381 taaagttggt atcaaaaccg aaaaatcggt cgtctattca gcttataaaa aaggtgttcc
     1441 gatcttcgtc cctgcgtttt cggattgttc ggctggtttc gggctaattc accatcaatg
     1501 gaatcgcaaa ggcaatatgg taacgattga ttccgctcgt gatttcctcg aactgactaa
     1561 aatcaaaatc gccgctaaag aaaccggcat tctaatgatt ggcggtggtg ttcccaaaaa
     1621 ttttactcag gacgttgttg tagccacaga cgtgctcgga atcgaaaaac caatgcataa
     1681 atatgctgtt cagttaaccg tggctgacga acgcgatggt gggctatctg gatcaaccct
     1741 caaagaagcc agttcctggg gcaaagtcga cactaagtac gaacaaatgg ttttcggcga
     1801 ggctacggtg atgatgccgc tcttggtcag ttatgcttat catcagggta attggaaaaa
     1861 acgccaggaa gcccgcttca gtaatctttt tgtttagtaa tcttaaccca aacagtcgtt
     1921 atactgaaat tatagtaagg gagtaaaagg ttaattggtc atctttcccc taccaaaaat
     1981 aatagattaa ttacactagt tgcttgatta tttttcccat ttagcacgat cgaggcggtc
     2041 taatttaatt gcgtctttga tgccaacaat tataaaaact ataatgaaaa ccccagccag
     2101 aatcgcaccg acaacgtcaa ctactttcca cggttgatta tttaacacca gcacggtagg
     2161 attccagaaa attagaattg tatttacta
//
LOCUS       DAOSMP010000150         2178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_50318_length_2178_cov_17.579840, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000150 DAOSMP010000000
VERSION     DAOSMP010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50318_length_2178_cov_17.579840"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..1582
                     /gene="gdhA"
                     /locus_tag="PKX09_10510"
     CDS             239..1582
                     /gene="gdhA"
                     /locus_tag="PKX09_10510"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOU18291.1"
                     /translation="MRYVSSVIESLQKRYPWEKEFLQAATEVLESLDEFVEINPKYKK
                     AKILERIVEPERVIIFRVPWVDDRGEVQVNVGYRVQFNSAIGPYKGGLRFHPAVTLSM
                     MKFLAFEQTFKNALTTLPMGGGKGGSDFNPTGKSENEIMRFCQSFMTELHRHIGPYLD
                     VPAGDVGVGGREIGYLFGQYKRIVNRFEAATLTGKGLAFGGSMLRPEATGYGAVYFVV
                     EMLKTKGETIKGKIVGVSGFGNVAWGVAKKATELGAKVISLSGPDGCIIDPDGVGTEE
                     KWNYMLEMRASGRDVVKDYADKFSVKFYPKARPWDIVPMDIAMPCAFQNEVSLENVQN
                     LIKNGCQILAEVSNMGCTPEAFKLANAKMMYAPGKAVNAGGVAVSGLEMSQNSERLSW
                     SAEEVDEKLKWIMTNIHSQCLAAAAEVGAPGNYAKGANIAGFRRVAEAMLEQGIV"
     gene            1799..>2178
                     /locus_tag="PKX09_10515"
     CDS             1799..>2178
                     /locus_tag="PKX09_10515"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOU18292.1"
                     /translation="MCGRKTLTKGKMEIIAELSIKEWDSSLDIQPNYNIAPTSIMPIL
                     VYENGRIVRAMRWGLIPEWAKDATSVPIMINARCESLTVKPSFRGLLDSQRCIVITDG
                     YYEWQKTESRKQPFYIHHPEKRLLT"
BASE COUNT          625 a          388 c          526 g          639 t
ORIGIN      
        1 ttactctaaa ttcttcggtc tattttgatg ccttattggc aaactatcat ttatatcaaa
       61 gagttacgag gttattccaa atctctttca attactataa atttttataa gttttattaa
      121 aatctttatt aatttttact cgtatgattt ccatattttt atcattcatt aaacaacatt
      181 cataaagatg ccccaaaatc aatcggtaaa catttatatt aaacaagatg gaggttaaat
      241 gagatatgtc agttcagtaa ttgaaagtct ccagaagcgt tacccctggg aaaaggaatt
      301 cctgcaagcg gctacggaag ttctggaatc gctggatgag tttgtcgaaa ttaatccaaa
      361 gtataaaaag gcaaaaatac tggaacggat tgtggagcca gaacgagtaa tcattttccg
      421 ggttccctgg gttgatgacc gtggtgaggt gcaagtcaac gttggttatc gagttcaatt
      481 caacagcgcg atcggtccct ataaaggtgg cttacgtttt catccggcag taacattaag
      541 tatgatgaaa tttttagcct ttgagcaaac ttttaaaaat gctttaacga ctttgccaat
      601 gggtggtggt aagggtggct cagatttcaa tccgaccggt aaatccgaga acgaaattat
      661 gaggttttgt cagagcttta tgacggaatt gcatcgtcat attggtccgt accttgatgt
      721 ccctgccggt gatgtaggag ttggtggtcg cgaaattggt tatctttttg gacaatataa
      781 gcggatagtg aatcgctttg aggcggctac tctgaccggc aagggtttgg cttttggtgg
      841 tagtatgctc cggccggaag ctaccggcta tggtgccgtg tatttcgttg tcgaaatgct
      901 gaaaaccaaa ggcgaaacaa ttaagggaaa aatcgtgggt gtttcaggtt ttgggaatgt
      961 ggcttgggga gttgctaaaa aagctactga attgggagcg aaagtaattt cattatctgg
     1021 tccggacggc tgtatcattg accccgacgg agttggaact gaggaaaaat ggaattatat
     1081 gcttgaaatg cgcgccagcg gtcgagatgt tgttaaagat tatgcggata aattcagcgt
     1141 taaattctat ccgaaagccc gtccctggga tattgttccg atggacattg caatgccttg
     1201 tgcttttcaa aatgaagtta gccttgaaaa tgttcagaat ttgatcaaga atggatgcca
     1261 gattctagct gaagtttcga atatgggttg tactccggaa gccttcaaat tagccaatgc
     1321 gaaaatgatg tatgctcccg gaaaagctgt caatgccggt ggggtggcgg tttccggtct
     1381 ggaaatgagc cagaattcgg aacgtttgtc ttggagtgcg gaagaagttg acgagaagct
     1441 gaaatggatt atgacaaata ttcattcgca atgtcttgcc gcagccgcgg aagtcggagc
     1501 tcccggtaat tacgccaaag gcgctaacat tgccggcttc agaagagtcg ccgaagctat
     1561 gcttgagcaa ggtattgttt agtttgaatt aaaatcgcgt attctatttt aataagcacg
     1621 gggttctgca ctataaaaaa tcttttatct tttgtcattt cccgacctga aatggtcaat
     1681 agtgggaaag gagatctttg attttacctc tcaagaaatg ttggttttta ccgagtaccc
     1741 agtttatatt aaaagatggt attagtcagg aattgatcaa ggtaaaaggc aggcttttat
     1801 gtgcggacga aagacactga ccaaaggcaa aatggaaatc atcgcggagc tttccatcaa
     1861 agagtgggat agttcactcg acattcagcc gaattataac attgcgccaa ccagcattat
     1921 gccgatttta gtatatgaga atggacgaat cgtgcgggcg atgcgctggg gtctaattcc
     1981 cgaatgggca aaagatgcta cctcagtgcc aattatgatc aatgcccgtt gtgaatcgct
     2041 aaccgttaag ccttctttta ggggattgct tgattctcag cgctgtatag taattaccga
     2101 cggctattat gaatggcaga aaactgaaag tcgtaaacaa ccattctaca ttcatcatcc
     2161 ggaaaagaga ttgctgac
//
LOCUS       DAOSMP010000151         2160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_50919_length_2160_cov_25.756770, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000151 DAOSMP010000000
VERSION     DAOSMP010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50919_length_2160_cov_25.756770"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(403..>2160)
                     /gene="dnaG"
                     /locus_tag="PKX09_10520"
     CDS             complement(403..>2160)
                     /gene="dnaG"
                     /locus_tag="PKX09_10520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOU18293.1"
                     /translation="DILEIVSDFVQLKQRGRNYFGLCPFHEEKTPSFSVNPSLGIFHC
                     FGCGKGGNAVTFIMEYEKIDYVEALKRLAERYSITIDWEGIEDSQKGEVALIYELHEI
                     AAEFYQKQLMTERGRSALEYIEKRGFDRETIEQFRLGYAPDEWESLFRQIDLNRFTPA
                     ILEKSGLFVRREGNKIYDRFRNRLMFPIINLAGRVVAFGGRALDPNEEAKYLNSPETP
                     IYFKSGIFYGLNFSKDAIQKAGDAIIVEGYTDFLRVYTSGFPNVVAGSGTALTLHHAR
                     LIHRFTKKVTLSYDGDEAGQKATERAGFLLMREGLDVRVIRLPESDDPDSYLRDHKPE
                     EFRELYDSANDFMTYYIDSHSDELNSPAAKTQFVERIAEELIEIKNPVILDLVVGEVA
                     ERINIKEEHIRAQIRYIARRRTNQNRGERSTESSKSLRLRTADEKAEYELLKMILTRH
                     QNLQTMIKNFLTAADFRHPVLHPIAEKLLSDLSSGKLAEADELFNTEWDEEQRLYLSR
                     LILEADPLQYVENMDDLRKLTSDCIAVLLTRDIDAEIAAVREKIKAAEKKGEDSTSLV
                     IQLSNLRQKKRALEDKIKA"
BASE COUNT          566 a          492 c          487 g          615 t
ORIGIN      
        1 atgactaaaa tgttttaaaa agtgactttt ttaaatggat caaccgtttg aaaacaatct
       61 caagccgttc tggctagtca tcaaataatt gggaatgatt aacgaaatga ttttgatatt
      121 tgccatagga gaaagtttaa ccccttcagg gtaaataata tttggatgaa ataatttcta
      181 caaaacttaa agccctacgg gctagttggg aatctatggt tattagataa acaatcaccg
      241 cagagccaaa accaatttag ctccgcctgc ggcgggtcgg aaattggttt cgttgcaaat
      301 tatgtcagcg cacaccgcgg agcgatcaaa gtattgtaga atataaaagt cagagaagtt
      361 attcctcgga gaggatcaag actttttcgc cctcagcaag gttcaggctt taatcttgtc
      421 ctcgagggcg cgctttttct ggcgtaggtt tgagagttga atcaccagtg aagtgctatc
      481 ttcacctttt ttctcagcgg ctttgatttt ctcccgcacg gcggcgattt cagcgtcaat
      541 atcccgcgtc agtaaaactg cgatgcaatc gctggttagt ttgcggagat catccatatt
      601 ctctacatat tggagcggat cagcctccag aatcaaacgt gataaataaa ggcgttgctc
      661 ttcatcccat tcggtattga aaagttcatc ggcttcggca agttttcccg agctcaggtc
      721 gctcagtaat ttttcagcga ttggatgcaa tacggggtgg cggaaatcgg cggccgtgag
      781 aaaattttta atcattgttt gcaaattttg atgtcgggtc aaaatcattt tcaggagttc
      841 gtattcggct ttttcatcag cagtgcgcag acgcaaactt ttcgagcttt cagtgcttct
      901 ctcgccccga ttttgattgg tgcgtcgccg ggcgatatag cgaatctgag cccggatatg
      961 ttcttctttg atattaatcc gctccgcgac ttcgccgaca accagatcca gaatgaccgg
     1021 attcttaatt tcgatcagct cttcggcaat gcgttccacg aactgagtct tggcggcggg
     1081 agaattgagt tcatcgctat ggctatcaat ataataagtc ataaaatcgt tagccgaatc
     1141 ataaagctcg cgaaattctt cgggtttatg atcgcgtaag taagaatccg ggtcgtcgct
     1201 ttccggcaga cgaataacac ggacgtccaa tccttcgcgc attagcaaaa atccggcgcg
     1261 ttcggtggct ttttggccgg cttcatcgcc gtcgtaactg agtgttactt tttttgtaaa
     1321 ccggtgaatc agacgggcgt gatgtaaagt caatgccgtt ccggaaccgg ctacaacgtt
     1381 ggggaagcca ctggtataaa ctcgtaaaaa atcggtgtag ccttcaacga taatcgcgtc
     1441 accggctttc tggatggcgt ccttggagaa atttaacccg taaaaaatgc cgctcttgaa
     1501 ataaatcggt gtttccggcg aattcaaata cttggcttcc tcattcggat caagcgctcg
     1561 cccgccaaag gcaacgactc gtccggctaa gtttattatc gggaacataa gccggttacg
     1621 gaagcggtcg tatattttgt ttccttctct gcgaacaaaa agaccggatt tttcgagaat
     1681 cgccggtgta aaacggttca aatcaatctg ccggaaaaga ctctcccatt catccggcgc
     1741 atagccgagc cgaaactgtt cgattgtttc gcgatcgaaa ccgcgtttct caatatattc
     1801 caatgccgaa cgaccacgtt ctgtcattaa ttgtttctga taaaattctg cggcaatctc
     1861 gtgtaattcg taaattaagg cgacctcgcc tttctgcgaa tcttcgatgc cttcccaatc
     1921 aatggtgata gaatagcgct cggctaatct tttcaaagct tcgacgtagt caattttttc
     1981 gtattccatt ataaaagtca ccgcgttgcc gcccttaccg caaccgaaac agtggaaaat
     2041 tcccaggctg ggattgacac tgaatgacgg agttttttct tcatgaaaag gacacagtcc
     2101 gaagtaattg cgcccccgct gtttcaattg cacaaaatct gaaacgatct cgagtatatc
//
LOCUS       DAOSMP010000152         2157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_51033_length_2157_cov_15.027117, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000152 DAOSMP010000000
VERSION     DAOSMP010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51033_length_2157_cov_15.027117"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..>2157
                     /locus_tag="PKX09_10525"
     CDS             196..>2157
                     /locus_tag="PKX09_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N'-diacetylchitobiose phosphorylase"
                     /protein_id="HOU18294.1"
                     /translation="MQFGHFDDKNKEYVIDRPDTPRSWSNYLGSTEYGAIITNNAGGY
                     SFFQSAAQGRFMRLRFNAIPMDQPGRYFYIHDLDKHDYWSASWQPVGKPLDKFRTVCR
                     HGTAYTIIESEYDNILTETTYFVPLNRHFECWLLKIHNRDNVTRRLRLFTFIEYTNNW
                     HVWQDTFNLQYTQFILRMNVVGNIIDHGINVNLTDSSGDLWHHDGNKHTFIGVVGAEV
                     SGFDTDREIFLGGYRSYHNPIVVEKGECLNSIAVGDNGVGVLQIDLELKPGESRELAV
                     LMGIGRAEVEGRKVLSEFQNLDNIHIEFEKLKEHWHRRVQGFLVQTPDSEFDSMTNVW
                     SPFNCLITYAWSRAASLIYSGERDGLGYRDTVQDLLGVLPLIPEEAGERLELMLTGQV
                     STGGAMSVVKPFDHHPGREIPPKEEEYRSDDCMWLFNTVPAYVKETGDLAFFDKVLPF
                     ADQGEATVLYHLRRAIEFNLERTGTHGLPCGLYADWNDCLELGQRGETIFVAFQLRYA
                     LKTYIEICELLERLSEKKWAVEQLQKLDENLRKYAWDGQWFLRAYRYDGLKYGSHTDE
                     EGSIWLNPQSWAVISGFADKDEAETAMQAVYERLATEYGLMILAPPYIKTDHRIVKAP
                     LFNAGMKENGSIFSHTQGWAIIAETMLGHG"
BASE COUNT          547 a          430 c          564 g          616 t
ORIGIN      
        1 agtcttgcag ttcaagcgcc ggagaccatt ttctggattc gggcgatgct ggcttgcatt
       61 cccattgtgg gattaatttt agtgattttt ttcataacca gggtaaacct cactcggcag
      121 aaatgcgcag agatccggtc agctcttgag gcgcgacgcg gtacggttta atttaaaata
      181 gtggagtaac taaatatgca gtttggtcat tttgacgata agaataaaga atatgtaatt
      241 gatcggccgg atactccgcg aagttggagc aattatcttg gttcgaccga gtacggtgcg
      301 ataattacta acaatgccgg tggctacagt ttttttcagt cggcggcgca agggcgattc
      361 atgcgtctgc gtttcaacgc gattccgatg gatcaaccgg gacgttattt ttatatccac
      421 gaccttgaca agcatgatta ttggtcggct tcctggcaac cggttggcaa accacttgac
      481 aaatttagaa ccgtctgtcg gcacgggacg gcttatacca ttatcgagtc ggaatatgat
      541 aatattttga cggagacgac ttattttgtg cctttgaacc ggcattttga atgttggtta
      601 ttgaaaattc acaaccgtga taatgttaca agacgtctgc gtctctttac tttcattgaa
      661 tacaccaata attggcacgt atggcaggat acttttaatt tgcaatatac tcagttcatt
      721 cttaggatga atgtagtcgg aaatatcatc gaccacggga ttaacgttaa tctaacggac
      781 agttctggtg atctctggca tcacgatggc aataaacata cttttatagg agtcgtgggc
      841 gcagaagtca gcggtttcga taccgaccgg gaaatattcc tcggtggcta tcgcagttac
      901 cataatccca ttgtggtgga aaagggcgaa tgtcttaatt ccattgccgt tggtgataac
      961 ggcgtaggcg ttttgcagat tgatcttgaa ctcaagccgg gtgaatcgcg cgaacttgcg
     1021 gttctgatgg gtattggaag ggcagaggtc gagggtcgga aggttttgag cgagtttcag
     1081 aaccttgata atattcatat tgaatttgaa aaactaaagg aacactggca cagacgggtg
     1141 cagggatttt tagtccaaac tcccgattct gaatttgaca gtatgaccaa tgtctggagt
     1201 ccatttaatt gtttgattac ttacgcctgg tcgcgagccg cgagcttgat ttatagtggt
     1261 gaacgcgacg ggctcggcta tcgtgataca gtgcaggatt tactgggggt tttgcctttg
     1321 attcctgaag aagccggtga acgtctcgag ttaatgttga ctggtcaggt ttccaccggt
     1381 ggcgctatgt cagtagtgaa gccgttcgat catcatcctg gccgggaaat tccgcccaag
     1441 gaggaagaat accgttcgga tgactgtatg tggttattca acactgttcc ggcttatgtt
     1501 aaagaaaccg gcgatttagc ttttttcgat aaggttttac cgtttgccga tcagggcgaa
     1561 gccacagtgc tctatcattt acgacgagcg attgaattta atttggaacg aaccggtact
     1621 cacggtttgc cctgtggact ttatgccgac tggaatgatt gtctggaact cggtcaacgt
     1681 ggcgagacga ttttcgtcgc ttttcaattg cgttatgccc taaagactta tattgaaatt
     1741 tgtgaactgc tcgagcgact cagtgagaaa aagtgggcag tggagcaatt acaaaaattg
     1801 gatgaaaatt tgcgcaaata tgcctgggac ggacagtggt ttctccgggc ttaccgctat
     1861 gatggtctga aatatggttc gcatactgat gaagagggtt cgatctggct caatccacag
     1921 tcatgggctg taatcagcgg gtttgccgat aaagatgagg cggagacggc gatgcaagct
     1981 gtttatgagc gcttggcaac cgaatatggt ttgatgattt tagccccgcc gtatatcaaa
     2041 accgaccatc gcatcgtcaa agcgccgctt ttcaatgccg gtatgaaaga aaacggctcg
     2101 attttctcgc atactcaagg ttgggcgata atcgctgaaa ctatgttggg acacggc
//
LOCUS       DAOSMP010000153         2145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_51407_length_2145_cov_13.774641, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000153 DAOSMP010000000
VERSION     DAOSMP010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51407_length_2145_cov_13.774641"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="PKX09_10530"
     CDS             <1..1308
                     /locus_tag="PKX09_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18295.1"
                     /translation="GAKFCWYSILHASGQPYDVPANDRDDFTQPKVTYIGTATKIPYN
                     DASDEYGNKNYWAIRGGYSKYWPMIGDTIPGTFHGGNSDENGSSDYSDFPAGTKSSPN
                     SKMSCSFGPYTFAPGQKIHIVYASGYSGLDLPTAQEVGKKWLNGTLEDPPNLPDSHTG
                     YLPSNFVFPIDATEMDLRKDRWVSTGVDSVMKSARRAKWNFDNGYKIPQAPPPPTYLE
                     ITGLGTGVEIKWRDPDAEAMPNFAGYRIMRRLSAMDTTFYQVIYDSGPDDKATDHLYI
                     DRDVLIGAQYYYYLQAKARIADDDPLADTTTRGKIIYSSRVLVPNINWINPPHFSQTD
                     LTKIRIVPNPYNINDPLLAAQGWTDQRGIQFFNLPGKVTIKIFTENGDLVQVLKHDSP
                     VEAGYYFWDMITLSQQVISSGVYIVVFQKPNGETAFQKFLVVR"
     gene            1305..>2145
                     /locus_tag="PKX09_10535"
     CDS             1305..>2145
                     /locus_tag="PKX09_10535"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOU18296.1"
                     /translation="MKAGGIIIKKLIYLTITCILILSLSSPINAQVGLKKVAQSTMNF
                     LQVGVAPRASAMGEAYCAFGTGAESIFFNPAGIVQSTHRFELQTYNTRWIADINYLAG
                     SFIWNLRQYGTLGLSMVSVDYGTIYGTSLLSAADKDLYPAGYIDLGNVDNVGSYAFGL
                     TYGRAISTQFLIGGSVRLVGQNLGQSQMADGLKNNDAGKLAFDAGVKYLTDFKSFRFA
                     MSIRNFSSNIKREEMDEQLPLLFTMGVAMDLLDVIAPTFSQNNSLNLAVDFQHPNNYS
                     ERIN"
BASE COUNT          555 a          504 c          504 g          582 t
ORIGIN      
        1 ggggcaaagt tttgctggta ttcgatcctg cacgcttccg gccaaccgta tgatgttccg
       61 gccaacgacc gcgatgattt tacccaaccg aaggtaactt atatcggcac cgcaactaaa
      121 attccttata acgacgccag tgatgaatac ggcaataaga attactgggc aattcgaggt
      181 ggttattcca aatattggcc aatgatcggg gatacaattc ccggaacctt tcacggtggc
      241 aacagcgatg aaaatggtag ttccgattat tccgactttc cggctggtac gaagagcagt
      301 cccaattcaa aaatgtcctg cagtttcggg ccttatactt ttgcgcccgg gcaaaaaatt
      361 catatagttt atgccagcgg ttactctggc ttggatttac caaccgctca ggaagttggc
      421 aaaaagtggc ttaatggcac tctcgaagac ccgcccaatc taccggattc tcacaccggc
      481 tatttaccat ccaattttgt ttttccaata gatgcgaccg aaatggattt gcgcaaagac
      541 cgctgggtta gtaccggcgt tgattcagtt atgaaaagcg cccgacgtgc taaatggaac
      601 tttgataacg gctacaaaat acctcaggct ccgccgccgc caacttatct cgaaatcacc
      661 ggtttgggca ccggggtcga aataaaatgg cgcgacccgg atgccgaagc tatgccgaac
      721 tttgccggct accggattat gcgccgcctg agcgcaatgg atactacctt ctatcaggtg
      781 atttatgatt ccggtcctga tgataaagcc accgaccacc tgtatattga tcgcgatgtt
      841 ttaatcggcg cgcagtatta ttactatctg caggctaaag cccgaattgc cgacgatgat
      901 cctttggcag atacgacgac tcgtggtaaa attatttaca gttcgcgggt gctggtaccg
      961 aatattaatt ggattaatcc ccctcacttt tcgcaaaccg atttgactaa aatccgtatc
     1021 gtccccaatc cttacaacat caacgatcct ttacttgccg cccagggctg gaccgaccag
     1081 cgcggcattc aattctttaa tctaccaggc aaggttacga ttaaaatttt taccgaaaat
     1141 ggcgacctgg tccaggttct caaacatgat agcccggtgg aagccggata ctatttctgg
     1201 gatatgatta ctctcagtca acaggttatc agtagtggtg tttatatcgt tgtgtttcag
     1261 aaaccgaacg gcgaaacggc ttttcagaaa tttctggtgg tacgatgaag gcgggaggaa
     1321 ttatcattaa aaaactgatt tatttgacta ttacttgtat tttgatccta agcctttcct
     1381 cgccaatcaa tgctcaagtc gggctgaaga aagtagcgca gagtacgatg aactttttac
     1441 aggtgggtgt cgcaccgcgt gccagcgcta tgggcgaagc ctattgcgct ttcggaaccg
     1501 gcgcggaaag catttttttc aatccggccg gcattgtcca atccacccat cggttcgaat
     1561 tgcaaactta caatacgcgc tggatcgccg acatcaatta cctggctggt agcttcatct
     1621 ggaatttacg ccagtacgga acgctgggat tgagtatggt ttcggtggat tacggaacga
     1681 tttatggcac tagccttttg tcagcggcgg acaaagacct ctatcctgcc ggttatattg
     1741 atttgggcaa tgttgataat gtcggctcct atgcgtttgg tctaacttat ggtcgcgcga
     1801 tcagcaccca gtttttaatc ggcggcagtg tacgtctcgt cggtcagaat ttaggtcaaa
     1861 gtcaaatggc ggacggcttg aaaaataatg atgcgggtaa actggcattc gacgccgggg
     1921 taaaatacct gaccgatttc aagagctttc ggttcgctat gtccattcgc aatttctcat
     1981 ccaatatcaa aagagaagaa atggatgaac aattaccgtt gctctttacg atgggggtag
     2041 caatggattt gctggatgtc attgcgccga cattctcgca aaataattct ctcaatttag
     2101 cggttgattt tcagcatccg aataactatt ccgaacggat taatt
//
LOCUS       DAOSMP010000154         2130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_51887_length_2130_cov_12.991807, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000154 DAOSMP010000000
VERSION     DAOSMP010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51887_length_2130_cov_12.991807"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /gene="trpS"
                     /locus_tag="PKX09_10540"
     CDS             complement(<1..700)
                     /gene="trpS"
                     /locus_tag="PKX09_10540"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOU18297.1"
                     /translation="MENRKVKGRILSGMRPTGKMHLGNYVGALENWVKLQDEYENYHL
                     IADYHALTTNLDTSELFSNTIEMVYDWLAVGIDPQRSPIFRQSQVKEHCELFLIFAML
                     ITTARLERNPTLKEQVRDLNIGTLSFGHLGYPVLQAADILIYKANLVPVGEDQVPHVE
                     ITREIARKFNQTWGKVFPIPEAKLTKFARLPGLDGNKMSKSVGNTILLADSDEEINSK
                     IKKAITDPQKIRRGD"
     gene            complement(708..1232)
                     /locus_tag="PKX09_10545"
     CDS             complement(708..1232)
                     /locus_tag="PKX09_10545"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOU18298.1"
                     /translation="MESKTNIINRNVLVLNQTYEPLHICDVKRAIVLILEDKASLVKA
                     FDHQMLKTVRQNFEIPSVIRIHKYIKVQHWEAVLNKANIFRRDNFTCQYCGARNVPLT
                     IDHIIPKVHGGQDTWTNLITACITCNNKKGNRNLIESGMVLRTKPIKPHKINTLQRFA
                     ESPIDEWRPYLFLE"
     gene            complement(1438..1902)
                     /locus_tag="PKX09_10550"
     CDS             complement(1438..1902)
                     /locus_tag="PKX09_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18299.1"
                     /translation="MGKRLKDVDLNNTNLFHQPRIVDIQNAHCPNGCDLMAENIKIKG
                     LKAIEVKIRWKNKEGKLYLDPEFGSYENISEVEVPKGEIVEFICPHCGISLRREQELC
                     RNCSAPTFAMELPGEGQIIGCLRNGCFEHTLKIESFAAMQLRIEEKFIRMIM"
BASE COUNT          600 a          415 c          413 g          702 t
ORIGIN      
        1 gatcgccgcg tcttattttc tggggatcag tgatagcttt tttaattttg gaattaattt
       61 cttcatccga atccgctaag aggatcgtat tgccgacgga tttactcatt ttattgccgt
      121 ctaaaccggg aaggcgggcg aatttggtta gtttggcttc cggtataggg aaaactttac
      181 cccaggtctg attgaatttc cgagcaattt cccgcgtaat ttccacgtgc ggcacttgat
      241 cttcgccaac cggtacaagg ttagctttat atatcaaaat atcggcggct tggagcacag
      301 ggtaaccgag atgtccgaac gacaaagtgc caatgtttaa atcacgcact tgttctttaa
      361 gggtagggtt acgttcaagg cgggctgtgg taattaacat tgcgaatatt agaaatagtt
      421 cgcagtgttc tttgacctga ctctgacgaa aaatcgggct gcgttgagga tctattccga
      481 ctgccagcca atcgtaaacc atctcaatcg tgttcgagaa caattcactt gtatccagat
      541 tagtcgtgag cgcatgataa tcggcgatga gatgatagtt ttcgtattcg tcttgtaatt
      601 ttacccagtt ttcgagagct ccgacgtaat tacctaaatg cattttaccg gtcgggcgca
      661 ttccgcttaa gattctgcct tttactttgc gattttccat tttgtcctta ttctaaaaat
      721 aaatatggcc gccactcgtc aattggtgat tcggcaaatc gttgtaatgt attaattttg
      781 tgaggcttga tgggtttagt tcttaatacc atccctgatt caattagatt acggtttcct
      841 tttttgttat tgcaagtgat acaggcggta ataagattgg tccaggtatc ttgaccgccg
      901 tgaactttgg gaataatgtg gtctatagtt agtggtacat tgcgagctcc acaatactgg
      961 caggtaaaat tatcgcgccg gaaaatattg gctttattaa gaaccgcttc ccagtgttga
     1021 actttgatgt atttgtgaat cctgataacg gaagggatct caaaattttg ccgaacagtc
     1081 tttaacattt gatgatcgaa tgccttgacc aggctggctt tatcctccag aataagcacg
     1141 atcgcccgtt tgacatcgca gatatgcaac ggttcatagg tctggtttaa aaccaggacg
     1201 ttccgattaa taatatttgt tttagattcc atgttcacaa cgcgttgata ttattgccta
     1261 acagccagaa cagctctgcg atcatattaa acattaaaaa atggctcaac taaacgacat
     1321 caaattttta tctgactaaa acttaattta aaaagcggta aaatttatac cgcaagttgt
     1381 attttcacaa caaaaaataa caagatatag tggcttaaaa atattcatca ttcgtagcta
     1441 cattatcatt ctaataaatt tttcttctat tcgcaattgc attgcggcaa agctttcaat
     1501 tttcaaagta tgctcgaaac aaccgttacg caaacagccg attatttgtc cttcaccagg
     1561 taattccatc gcaaaagtcg gagcagaaca attgcgacag agctcttgtt cacgtcgcaa
     1621 agatatccca caatggggac agatgaactc gactatctcg ccttttggta cttcgacttc
     1681 tgaaatgttt tcgtaactgc cgaattctgg atcaagatac aacttacctt ctttattttt
     1741 ccaacgaatt ttaacttcaa tggctttcaa gcccttaatt ttgatatttt ctgccattaa
     1801 atcacagcca ttgggacagt gtgcattctg aatatcaact attcggggtt gatgaaaaag
     1861 gtttgtattg tttagatcaa catcctttag tcgttttccc attgttaatc ctattctaaa
     1921 tctcaaataa tcttggcaaa atttacaaat attttactta atgcctacaa ccggtttaga
     1981 actaattaat atttttttgc caagctcata actatgaatt acgcaaatag ttttttgttc
     2041 tttaaatgaa tgtaagtcgg tatttataga taattaaaaa caggctaaaa agcctgttct
     2101 acgttttggt gatgtggagc tggtcgggat
//
LOCUS       DAOSMP010000155         2114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_52430_length_2114_cov_13.310345, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000155 DAOSMP010000000
VERSION     DAOSMP010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52430_length_2114_cov_13.310345"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..>2114
                     /locus_tag="PKX09_10555"
     CDS             112..>2114
                     /locus_tag="PKX09_10555"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HOU18300.1"
                     /translation="MQKRKRVSTFSIVVLTLGLFTFPALTGAKGATYYVSSNGNDDLD
                     GTSWETAYKTIQQAINNSLDYDTIIVGSSGGHGDSVYTENVNVNKRLIIKSESGYATT
                     TVVAADTSDHVFEITASDVTIGGEDCGFSIYNATGEEKAAIFLDSTVTGCTIQGNRCG
                     WDNEHKNYCGILLYSSNTNTISNNTSSSSTIGICLLISFNNTISGNTLRSNNFGISLA
                     ANSNNNTISGNTCNSNSEYGLSLNLSKYNIIYLNNLSNNSDGNTSVSDSPTNTWNSPT
                     PIFYNYTGGSYHKNYLGNYYGDYTGPDDDGDGIGNSSYTEENGTTDNFPLKATSDHYS
                     LQAWWLSGDSKMYRADMTKSPGVVAITGGSSQIWSAEEATLANISFSGTDGWTGQVIF
                     TTPIADKDSFKIEIGYSTNGSDFNTGGPNATIIGDGSDSVFTYQTNASAFTIPEGNYL
                     ALKITNNSSNSYSVQTGGASSYCSAPNQSQDYSLPVELASFNATAGNGNITLKWITES
                     EIENLGFNIYRSTSRDGEFVMLNAKLIAGARSSSERHAYSYIDRDVKSGLTYWYKLED
                     VDYNGKTEFHGPISAVLANTTPTEFGLHKNYPNPFNPVTTISYDLAKDVYVELAVYNM
                     LGGKVITLVNGNQPAGSYQLEWDGRDSRGLIVSSGMYLLRINAG"
BASE COUNT          643 a          470 c          448 g          553 t
ORIGIN      
        1 ctggatctaa aaaagcttga tattttcatt taaataatta aaattgctat gtgaatttgc
       61 aacttaaacc aatgggcatt ttcagttcat ataaaatttg aggggcgaaa aatgcaaaaa
      121 agaaaaagag tgtccacgtt ttcaatcgta gttttaactc tgggcctgtt cacctttcca
      181 gcgctaaccg gtgctaaggg agctacttat tatgttagtt caaacggtaa cgatgacttg
      241 gatggtacca gctgggaaac cgcttataaa accatccaac aagcaataaa taattcccta
      301 gattacgata ctattatcgt cggcagtagt ggtgggcatg gcgacagcgt ttataccgaa
      361 aacgtgaatg tgaacaaaag actaatcatc aaatcggaga gtggctatgc taccaccacc
      421 gttgtagccg cagatacaag cgatcatgta tttgagatta ctgctagcga tgtgaccatc
      481 ggcggcgaag actgcggctt ttccatctat aatgcgaccg gcgaggaaaa agccgcgata
      541 ttcctcgaca gtaccgtaac tggttgcaca attcagggca accgctgtgg ctgggataat
      601 gagcataaga actactgtgg catattgctg tatagttcaa ataccaatac catctcaaat
      661 aataccagtt cttcatcaac tataggtatt tgcctgctta tatcgtttaa taatactatc
      721 tcaggtaata cccttagatc aaacaacttt ggtatcagcc tggcggcaaa ttctaataac
      781 aacaccatct caggtaatac ctgtaattca aatagtgaat atggtttatc cttgaatttg
      841 tcgaaatata atattattta cctgaacaat ttatccaata attctgatgg aaatactagt
      901 gtttccgata gccccaccaa cacctggaac tcgcccaccc caatattcta taactacacc
      961 ggcggctctt atcataaaaa ttaccttggt aattattatg gcgattacac tggacctgat
     1021 gacgatggcg atggaattgg aaatagttct tacacggaag aaaatggaac tacggataac
     1081 ttccccttga aggcaacctc tgaccactat tccctccaag cctggtggct cagcggcgac
     1141 agcaaaatgt atcgagccga tatgactaaa tcgccaggcg ttgttgctat aaccggcggt
     1201 agttcgcaaa tctggagcgc tgaggaagcc acccttgcca atattagctt ttcaggaaca
     1261 gacggatgga ccggtcaggt gattttcaca actccaatag cagataaaga ttctttcaaa
     1321 atcgaaatag gctattccac caatggaagc gattttaaca ccggcggtcc caatgctacc
     1381 ataatcggag acggcagtga ttccgttttt acttatcaga ccaatgccag cgcctttacc
     1441 attcccgaag gaaactactt agcgctcaag attacaaaca attcaagtaa cagttacagt
     1501 gtgcaaactg gtggtgcctc gagctactgt tccgcaccga atcaaagtca ggattattcc
     1561 ctgccggtcg aactggcttc cttcaacgct actgccggaa atggaaatat taccctcaag
     1621 tggattaccg aaagtgaaat cgaaaacctg ggcttcaata tctaccgtag taccagtcgg
     1681 gatggtgaat ttgtaatgtt gaatgctaaa ttgatcgccg gagccagaag ttcatcggaa
     1741 aggcacgcgt atagttacat tgatcgggat gttaaaagtg ggctaaccta ctggtataag
     1801 ctggaggacg tggactataa tggcaagacc gaattccacg gaccaatctc cgcggtgctg
     1861 gcaaatacta ccccgaccga atttggcttg cacaaaaact atcccaatcc gttcaatccg
     1921 gtgacaacca tttcctatga cttagccaaa gatgtttacg ttgaattagc agtttataac
     1981 atgctgggag gaaaggttat tacactggtc aatggcaatc agccagctgg ttcatatcaa
     2041 ctcgaatggg atggcaggga cagccgcggc ttaatcgtat cttccggaat gtacttactt
     2101 agaatcaacg ccgg
//
LOCUS       DAOSMP010000156         2093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_53128_length_2093_cov_10.357213, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000156 DAOSMP010000000
VERSION     DAOSMP010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53128_length_2093_cov_10.357213"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /locus_tag="PKX09_10560"
     CDS             complement(<1..1231)
                     /locus_tag="PKX09_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18301.1"
                     /translation="MQKFKIIIIAGVAFLFSFCDLKIPSESDFPTWSVNLNVPLLNET
                     VTVNDLLEDSLIVKLPYGTSGDSIFAYEDQIPIEEVRVGNRLNIDDITQSIQQGVDEV
                     NIAASDKKYSSGFDEVGVEPVSEQNSNLIGDIHLADTDVESTAPILFSEIVDMSGIAD
                     GASKTIAINTPFPTTKRQIVFNNFNNANFIGGFLEINIRNDLVVELGAPVYVRLLDGS
                     NNPIVGTDGDSAKAVWTEGLADGQSSKKTINLINKTLPGTIIVEVSGVICGSASDNVT
                     KNPTTLNSSFVVEVQAKDISVSSAEAIVPEQIIDTTGTIEFAEDEPNKVETAVIKDGR
                     LKISIQNNLPVSANLELTIPSLKTASGGSDFYQVIPMAANQTVIKEYLLTGHELEMDI
                     DEQEVNYSYIVRTISTDP"
     gene            complement(1369..>2093)
                     /locus_tag="PKX09_10565"
     CDS             complement(1369..>2093)
                     /locus_tag="PKX09_10565"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOU18302.1"
                     /translation="AIYSTFLQRAYDNIIHDVALQNLPVVFVLDRGGIVGEDGPTHHG
                     AFDLSYLCTVPNMIVTAPRNGTELRRLMFTALEQNDAPFAIRYPKDSYVEFDDQAPAV
                     PYQIGKWEIIHNGQDVAILSVGVMTNNAIEAACLLKSKNIFPTIVHARFVKPLDTQML
                     SEIASKHRIIITIEENSIVGGFGEQVARYFAEQNLPQKISILAIPDHFIEQGAREILL
                     RDLALDAVGIANKVEQIYHKLE"
BASE COUNT          610 a          458 c          397 g          628 t
ORIGIN      
        1 cgggatcggt tgaaatggtt cgaactatat aagaatagtt gacttcttgc tcgtcgatat
       61 ccatttctaa ctcatggcca gttagcaaat attccttaat tacagtctgg ttagccgcca
      121 ttgggataac ttgataaaag tcactgccac cagaagcagt ttttaaagaa gggatggtga
      181 gttcgagatt agcgctgacc ggcaaattat tctgaataga tattttcagg cgaccatctt
      241 taatcaccgc tgtttcaact ttattgggct cgtcttccgc aaactcaatt gtgccagtag
      301 tgtcaatgat ctgttctgga acaatcgcct ccgccgaaga tacagatatg tccttagctt
      361 gaacttccac cacgaatgaa gaattcaagg tcgtgggatt tttcgtaaca ttatccgagg
      421 ctgaaccaca aataactccc gaaacctcca cgatgattgt cccgggcaaa gttttattaa
      481 ttagatttat tgttttttta ctggattgcc cgtctgccaa accctcagtc cagacggctt
      541 tagccgaatc tccatccgtt ccgacaatcg gattattaga gccatccagt aagcggacat
      601 ataccggtgc cccgagttct actaccagat cgttgcggat attaatttcg aggaatcctc
      661 caataaaatt agcattatta aaattattaa aaacaatttg tctttttgtc gtcggaaaag
      721 gtgtatttat ggcgatggtc ttgctggctc cgtccgctat accgctcata tccactatct
      781 ccgaaaataa aatcggagcc gtgctttcga catcggtatc agccagatga atgtcaccga
      841 ttaggttgct attctgttca gaaaccggct caactccgac ttcatcaaaa cccgaagagt
      901 attttttatc acttgcggca atattgactt catctacacc ctgctgaatg gattgggtta
      961 tatcatcaat atttaaccta ttaccgaccc gaacttcttc aattgggatt tgatcttcat
     1021 aggcaaaaat tgaatcacca gaggtaccat aaggaagctt gactatcagc gaatcttcta
     1081 ataaatcatt gaccgtaaca gtctcattta atagcggaac gttcaaattt accgaccaag
     1141 ttggaaaatc cgattcgctg gggattttaa gatcacaaaa tgaaaataaa aacgctactc
     1201 cggcgatgat gatgatttta aacttttgca tgattaccct cattaaaact caaatataat
     1261 aacttatttt ataacaatta ataatagaat tatgtgacat aagtctcact taaacgttcc
     1321 taatgtaaat aaacaactta cctcttcgtt ttttacaatg ttatctaact attcaagttt
     1381 atgataaatt tgctcaactt tattcgctat cccgacggca tcaagcgcta agtctctcag
     1441 taaaatttca cgcgctcctt gttcaataaa atggtcggga attgccagaa tgctaatctt
     1501 ttgcggtagg ttttgttcgg caaaatatct ggcaacctgt tccccgaatc caccaacaat
     1561 gctgttctct tcaatcgtga taataatgcg atgcttactg gcaatctccg acaacatttg
     1621 agtatcaagt ggtttcacga aacgagcgtg gacaatagtc ggaaatatat tttttgattt
     1681 aagtaggcag gccgcctcta tcgcattatt agtcattacg ccgaccgaca agatggcgac
     1741 atcttgacca ttatgaatta tctcccactt tccaatttgg tagggcacgg ctggcgcttg
     1801 atcatcaaat tcgacataag aatcttttgg atagcgaatg gcaaatggcg catcattctg
     1861 ctcaagagct gtgaacatta atctgcgcaa ttcagtgccg ttccgtggcg cagttacgat
     1921 catattgggc actgtgcata aatagctcaa gtcaaatgca ccatgatggg tcggaccgtc
     1981 ttcgccgacg attcctcctc gatccaggac aaatactacc ggtaaatttt gtaaagccac
     2041 atcatggata atattgtcgt aagcgcgttg taaaaaggta gaatagattg cca
//
LOCUS       DAOSMP010000157         2049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_54687_length_2049_cov_17.005015, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000157 DAOSMP010000000
VERSION     DAOSMP010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54687_length_2049_cov_17.005015"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..370
                     /locus_tag="PKX09_10570"
     CDS             8..370
                     /locus_tag="PKX09_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOU18303.1"
                     /translation="MYKCEICGNIVEVLHEGAGELVCCGKPMKLIVENTTDAAREKHV
                     PVIEKIADGYKVSVGSVLHPMIDVHYIEWIELDADGQVFRKYLKPGDTPVAIFNVSAE
                     NVTAREYCNIHGLWKGTL"
     gene            370..858
                     /locus_tag="PKX09_10575"
     CDS             370..858
                     /locus_tag="PKX09_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOU18304.1"
                     /translation="MLSPKLLTAFNDQIKHELESAYLYAAMNAWFKSENFDGMAHWME
                     VQTKEEINHARKFFDHIFERGDVVELQPINILGKKWASPLDAFKAAYEHEQFVTSKIN
                     DLMTMAIADKDYAAQEFLNWFVAEQVEEEDSTLKIVQLLKRIGDHGQALIMVDRELGK
                     RE"
     gene            875..1357
                     /locus_tag="PKX09_10580"
     CDS             875..1357
                     /locus_tag="PKX09_10580"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOU18305.1"
                     /translation="MNFNIDEVFEIAEQIEVNGSRFYRQAAELSQLAEHKNMLLKLAE
                     MEDQHQKTFAGMREKIKQTKLNLSYDPNQEAALFLQAFASGYVFDPTADSSALIKDQK
                     SMADILHLAIGFEKDSIVFYLGIEKILLNEDDIVHVRAIIKEEMGHITLLARKLDELN
                     "
     gene            1506..1994
                     /locus_tag="PKX09_10585"
     CDS             1506..1994
                     /locus_tag="PKX09_10585"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HOU18306.1"
                     /translation="MAIRIFVTGGTFDKEYNELTGQLYFKDTHLPEMLILGRSRVEVE
                     IRTLMMIDSLEMTDDDRLLIAQHCQKAAEDRIVITHGTDTMVETARLIAQNVSGKTIV
                     LTGAMIPYKFGSSDGLFNLGSALAFVQTLSPGVYIAMNGRFFSWNNVRKNKKTGEFEE
                     IS"
BASE COUNT          631 a          407 c          460 g          551 t
ORIGIN      
        1 atctcaaatt tacaaatgtg aaatctgcgg caatatagtc gaggttttac acgaaggcgc
       61 gggcgaattg gtttgctgtg gcaaaccgat gaaacttatc gtcgaaaata cgactgacgc
      121 cgcccgagaa aaacatgtcc cggtaattga aaaaatcgcc gacggctaca aggtcagcgt
      181 cggtagcgtt ctccacccga tgattgacgt tcattatatt gagtggattg agttggatgc
      241 cgacggtcag gtttttcgga aatatctgaa gccgggcgat acaccggtgg ctatttttaa
      301 cgtatcggct gaaaatgtaa ccgcacgtga gtattgcaat attcacggct tatggaaagg
      361 aacattataa tgcttagtcc caaattatta accgctttca acgatcaaat caaacacgaa
      421 ctggaatccg cctatcttta tgccgcaatg aatgcctggt tcaaatccga aaacttcgac
      481 ggaatggcgc attggatgga agtccagacc aaggaggaaa taaaccatgc caggaaattt
      541 ttcgaccata ttttcgagcg tggtgatgtc gtcgaactcc agccaataaa tattttgggt
      601 aagaaatggg cttcgccgct cgatgctttc aaagccgctt atgagcacga acaatttgtt
      661 accagcaaaa tcaatgatct aatgacgatg gctattgccg ataaagatta tgccgcacag
      721 gaatttttaa attggttcgt cgctgaacag gtcgaagaag aagattcaac tttgaaaata
      781 gttcaacttt taaagagaat cggcgatcac ggtcaggctt tgattatggt tgatcgagaa
      841 cttggtaagc gcgaataaca ggaaagagcc aattatgaat tttaacatag acgaagtttt
      901 cgaaattgcc gagcagatag aagtcaatgg cagccgtttt tatcgtcaag ccgctgaact
      961 cagccagttg gctgaacata aaaatatgtt attaaagttg gctgagatgg aagatcagca
     1021 ccaaaaaacc ttcgccggca tgcgtgaaaa aataaaacaa accaagctaa atttgtctta
     1081 tgatcccaat caagaagccg ctctcttttt acaagccttt gcttctggat acgtgtttga
     1141 tccaacggct gattcatctg cgttaatcaa agatcaaaaa tcaatggcgg atattttgca
     1201 cttagccatt ggatttgaaa aggattcaat cgttttctat ctcggcatcg aaaaaatcct
     1261 gctcaacgaa gacgatatcg ttcacgtacg tgctattatt aaagaagaga tgggacatat
     1321 taccctgctt gcccgaaaat tagacgaact gaattaagct aaacggcaat ttgttttgat
     1381 ttaataccct tacaggtatg attacttgtg agggtatttt tttgtataat atccttgatg
     1441 taattaatgt aggctgttta aattgacgat aatgattaac actaaaataa ttaaaggaat
     1501 aaaaaatggc aatccgtatt tttgtaacgg gcgggacttt tgacaaagaa tataatgaac
     1561 tcaccggaca actctatttc aaagacacgc acttaccgga aatgctaatc ctcgggcgct
     1621 cgcgggtaga agtcgaaatc cgcaccttga tgatgattga tagtctggaa atgaccgatg
     1681 atgaccggct attgattgcg caacattgtc agaaagccgc tgaagatcgg attgtcatta
     1741 ctcatggcac ggatacaatg gtagaaaccg cccgtctcat tgcccaaaat gtcagcggca
     1801 aaaccatcgt cttgaccgga gcgatgattc cctataaatt cggtagttcg gacggactct
     1861 tcaatctcgg cagtgcttta gcttttgttc agacactttc tcccggcgtt tacattgcga
     1921 tgaatggtcg cttttttagt tggaataacg ttcgtaagaa taaaaagact ggcgaattcg
     1981 aggaaatatc ctgattgttg agcgagagac gggacttgaa cccgcgatcc cgaacagtcg
     2041 ggagcaacg
//
LOCUS       DAOSMP010000158         2029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_55423_length_2029_cov_11.823708, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000158 DAOSMP010000000
VERSION     DAOSMP010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55423_length_2029_cov_11.823708"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PKX09_10590"
     CDS             complement(<1..796)
                     /locus_tag="PKX09_10590"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOU18307.1"
                     /translation="MKKCKPIQLAMVLLLFLSQIFAQFVDVKVEISVDRMPELERSDL
                     KILEMAIPAYYENYEWIENTYGIQIPLKINIYPQSVNTTGSERIFTAQLFIVNESGDM
                     RFFEKNFKFVYNTNDPIVHSEMIQTLTGALDFYAWMMIASEMDTYEPLGGNAYYEKAR
                     DVATRGQMSERPAGWKDRLQELDEILRLRNYRLMKYHFWAAMDYADQGNREAVAPEID
                     KALEAIEAIFAENARERYSHIFLDAHARDIMTIIKEFGTIEQAHKLM"
     gene            984..>2029
                     /locus_tag="PKX09_10595"
     CDS             984..>2029
                     /locus_tag="PKX09_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18308.1"
                     /translation="MSSSRNKLTWAVFLLTVGWLIPLWAQTRLTGGKRPSWIDHPAPG
                     YYIGISRRWAEEADSRADALNNAKRQIIESLGGVIESEFIDRIIETDGTVESVDAFTD
                     SRIKVVAKNIINVKPEKVYTEQWQEGKGRQKKILYQTYMAVYFDENAHRAFMKQLVEE
                     TVHLGEIQLSSAITLARQGRIFLAIDQIKAATQHLQPLTEITGIAPTDLIKIKTLNGQ
                     MLDWINLLQNGIRIEGLGDKQTTKWGAGLPEPLQVSVFWQESGKRYPISGLPVEFKLL
                     AGKAAFNPYSQTDASGCAFCSVKEIRTAGKVEFEAQVTFPEGYQMAQTSTRFNLLPDN
                     KVIVMVSETNFYHPV"
BASE COUNT          556 a          455 c          467 g          551 t
ORIGIN      
        1 ccatcagttt gtgagcttgt tcgatagttc caaattcctt gatgatcgtc attatatcgc
       61 gggcatgggc atcgagaaaa atatgcgaat atcgttcgcg ggcattttcg gcaaatatcg
      121 cctcgatggc ttccaaggct ttgtcaattt cgggggcaac cgcctctcta tttccttgat
      181 cggcatagtc cattgccgcc cagaaatgat atttcattaa tcggtaattt cgcaggcgga
      241 gaatctcgtc caattcttgc aggcggtctt tccaaccggc gggtcgttcg gacatttgcc
      301 cgcgggttgc tacgtcccgc gctttctcat aataggcatt gccgcccagc ggttcgtagg
      361 tatccatttc gctggcaatc atcatccaag cataaaagtc cagcgcaccg gtcagggttt
      421 gaatcatttc ggaatggact atcggatcat tagtattata gacaaacttg aaatttttct
      481 caaaaaaccg catatcgcct gattcattga caatgaacag ctgggcagtg aaaatccttt
      541 cggaaccggt cgtattgaca ctttggggat agatattgat ttttagtgga atttgaatgc
      601 cataagtgtt ttcgatccat tcgtagtttt cgtaataggc agggattgcc atttcgagaa
      661 ttttcaggtc cgagcgttcc aattccggca tccgatctac cgatatttcg actttgacgt
      721 ccacaaattg agcaaatatt tgacttagaa aaagtaacaa taccatcgcc agctggatag
      781 gtttgcattt tttcattaga atttcccctt gtaattgttt ttaatcacgt ttggtattta
      841 tttcactcat tattctgcgc cattaatata atactttagc ggcaaagtta aaactgccag
      901 tttccgtgcc agataagttc ggtttgaaat tataaaactt tattggtaga tttaattaac
      961 agagctgaaa aaaggaaagc gagatgtcga gtagcagaaa caaattaacc tgggcagtct
     1021 ttctactgac ggtaggttgg ctaattccgc tttgggctca aacgcgatta accgggggaa
     1081 agcgtcccag ctggattgac cacccggcgc ctgggtatta catcggcatt tcgcgtcgtt
     1141 gggcagaaga agctgacagc cgcgccgatg ctttgaacaa cgctaaacgt caaataatcg
     1201 aatccctcgg cggcgtcatt gaaagtgaat ttattgatcg catcatcgaa accgacggta
     1261 cagtggaatc agtggacgct tttaccgatt cacgcatcaa agtcgtagcc aaaaatatta
     1321 taaacgttaa gccggagaag gtctatacgg aacagtggca ggagggcaaa ggtcgtcaaa
     1381 agaaaattct atatcagacc tacatggcgg tttacttcga cgaaaatgct caccgcgctt
     1441 ttatgaagca attagtggag gaaaccgttc atctgggcga aatccaactg agttcagcaa
     1501 tcaccttagc ccggcaaggt cgtattttcc tggcaattga tcagataaag gcggctactc
     1561 aacatttaca accgctgacc gagattaccg gaatagcgcc gactgatctg ataaaaataa
     1621 aaaccttaaa cggacaaatg ttggactgga ttaatctctt gcaaaacggg atccgcattg
     1681 aaggtctggg tgataaacaa actaccaaat ggggcgccgg attacctgaa ccgttgcagg
     1741 tgagcgtatt ttggcaggaa agtggcaagc gctacccgat ttccggactg ccagtcgagt
     1801 ttaaattgct ggcgggcaaa gccgcattta atccctacag tcaaaccgat gccagcggct
     1861 gtgccttttg ttcggtcaaa gaaatccgta ccgctggcaa agtcgaattc gaggctcagg
     1921 ttacttttcc cgaaggctat cagatggcgc aaaccagtac ccgctttaac cttttaccgg
     1981 acaacaaggt aatcgtaatg gtctctgaaa ccaattttta ccacccggt
//
LOCUS       DAOSMP010000159         2024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_55605_length_2024_cov_12.409345, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000159 DAOSMP010000000
VERSION     DAOSMP010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55605_length_2024_cov_12.409345"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PKX09_10600"
     CDS             <1..344
                     /locus_tag="PKX09_10600"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOU18309.1"
                     /translation="DVYNFGQMKRDFTYIDDIVAGLRSAIDKPFPYEIFNLGNHHSEN
                     LMDFIHLIEEYSGRKAQINFQPIQPGDVPETYADISTATEKLGFRPRTDIREGIKLFL
                     DWYFEYYHIKK"
     gene            350..1333
                     /gene="rfaE1"
                     /locus_tag="PKX09_10605"
     CDS             350..1333
                     /gene="rfaE1"
                     /locus_tag="PKX09_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HOU18310.1"
                     /translation="MLRTIPADLKNLDKIFKQIQNLHILVVGDLILDQYIWGKVERIS
                     PEAPVPIITVSRKENRLGGAGNVAANLSALECPVTLAGVCASDQAGRDLQTLCHQKKI
                     NSIIATLPERRTSCKARIIAQQQQLLRIDEETIEPVAKKETEQLLKNITALANQFDAV
                     ILSDYAKGLVTEDFIQSILEIFKDKPVLVDPKGFNYQKYRGATALKPNFREFCMAVRH
                     PELTLAKIEPLARQLIDELNLKGLIVTLGEQGVFVLDTDQKAALIPTKAREVFDVSGA
                     GDTFIAVFTAVLMVTNDWKIAAVVANLASGIAVGKIGTATVTAAEILAEDY"
     gene            complement(1534..>2024)
                     /locus_tag="PKX09_10610"
     CDS             complement(1534..>2024)
                     /locus_tag="PKX09_10610"
                     /inference="COORDINATES: protein motif:HMM:NF020752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18311.1"
                     /translation="RQKLNFSFEKLEESGKIITRLRDFKKSLEGEFPTGNVGTKEIVE
                     KCETDFETALDDDLNISNALAAVFKLLHEINLYRQENPLTQADVELIRQSLKRIDSVL
                     NILPEPLDMLSEEERKLIDARNQARRERNWAEADRLRNLLLERGIILEDTPKGTTWKR
                     KI"
BASE COUNT          567 a          466 c          428 g          563 t
ORIGIN      
        1 ttgacgtcta taattttggt cagatgaagc gcgattttac ttatattgat gatatcgtcg
       61 ctggtttgag gtcggcaatt gacaaaccct ttccgtatga aatttttaat ctcggaaatc
      121 atcacagtga aaacttgatg gatttcattc atttaatcga agaatattcg gggagaaaag
      181 ctcagataaa ttttcagccg attcaacccg gcgatgtacc ggaaacctat gccgacatct
      241 ccaccgcaac ggaaaaactc ggcttcagac cgcgcacgga catccgggaa ggtatcaaat
      301 tattcctcga ttggtatttt gaatattatc atattaaaaa gtaaaaaaca tgcttagaac
      361 aattcctgcc gatttgaaaa atttggataa aattttcaag caaatccaaa acctgcatat
      421 tctagtggtt ggcgacctga ttctcgacca atatatttgg ggtaaggtcg aacgcatttc
      481 accggaagca cccgtgccta ttataactgt cagccgtaaa gagaatcgcc tcggcggagc
      541 cggcaacgtc gccgccaact tgagcgcttt ggaatgtccg gtaactttag ctggtgtgtg
      601 cgcttccgat caagctgggc gggatttaca gactttatgt caccagaaaa aaattaactc
      661 aattatcgcc acattgcccg aacggcgtac atcttgtaaa gcgcgaatca tcgcgcaaca
      721 gcaacaattg ctccgcattg acgaagaaac catcgaaccg gtagccaaga aagagaccga
      781 gcaacttctc aaaaatatta cggctttagc caatcaattc gatgccgtaa ttctttccga
      841 ttacgccaaa ggtctggtca cggaagactt tatccaatcc attctggaaa tattcaagga
      901 taaaccagta ctggtcgatc ccaagggttt caactatcaa aaatatcgtg gcgcgaccgc
      961 cctcaagccc aatttccgtg aattttgtat ggcggtgcgt catcccgaat tgaccctcgc
     1021 taaaatcgaa ccgcttgctc gacaattgat tgatgaactt aacctgaagg gtttgatcgt
     1081 tactctgggc gaacaagggg ttttcgtact cgatactgac cagaaagccg ctctaattcc
     1141 cactaaggcg cgcgaagtct tcgatgtcag cggtgccggt gatactttta tcgccgtttt
     1201 caccgccgtt ctaatggtta ctaatgattg gaaaatagca gctgtagtag ctaatttagc
     1261 gtccggcatt gcagtcggaa aaatcggtac ggctaccgtc acagccgctg aaattctcgc
     1321 tgaagattat tagctattca ccactaaggc acaaagaaca ctaagaaata ttcggtattt
     1381 ttaaaattga ttccattagt ttttcttccg gcggaacatt tacggcataa tacgggagaa
     1441 aaatttccca aacttttata accaacaaga aatcaacccg acgctcggga tttagagttt
     1501 agtttctttt gaatttattg gccttttgaa aactcagatt ttgcgtttcc aggtcgtacc
     1561 cttgggcgtg tcttcgagaa taatgcctcg ctcgaggaga aggtttcgca agcgatcggc
     1621 ttccgcccaa ttacgttccc gccgggcttg atttctggca tcaattaatt tacgctcttc
     1681 ttcagaaagc atgtcaagcg gttcgggcag aatgttcaga acggaatcaa ttcgttttag
     1741 agattgccga attaattcta catccgcctg ggtcagcgga ttttcctgac gatataaatt
     1801 gatttcgtgt aataacttaa aaacagccgc cagggcattg gagatattca gatcatcgtc
     1861 gagcgctgtc tcaaagtcag tctcacattt ctcgacgatt tccttagtgc caacatttcc
     1921 cgtcggaaac tcaccttcca gtgatttctt aaaatcgcgc aagcgcgtaa tgatcttgcc
     1981 ggactcttcg agtttctcga aactgaagtt taatttctgc cgat
//
LOCUS       DAOSMP010000160         2021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_55764_length_2021_cov_6.971007, whole genome shotgun sequence.
ACCESSION   DAOSMP010000160 DAOSMP010000000
VERSION     DAOSMP010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55764_length_2021_cov_6.971007"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2002)
                     /locus_tag="PKX09_10615"
     CDS             complement(<1..2002)
                     /locus_tag="PKX09_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18312.1"
                     /translation="MDVYNYMSGFWRDGSPITEGGVGTDPSKPPTKFMFSGDPETGTG
                     WLDSNPADRRFFMTTGPFTMEPWVDSDGDGIAEFGEPGVQEIVAGVIVARGSSNSNSV
                     TTLKEVDNLAQLAYELNFILAKAPAAPEVTVSELPNEVVLTWSGNSEYNDDGSLYDSP
                     DPIVAQAFGDTVIIDNTEKVITDSTYNFYGYTVYQYSDASGADPVKVESWDIGKNADP
                     MEYTKQRFIRLLVNKHPKVGNVNDPLINGKEYYFGVVAEGYLEFGAPQIFSSSPTIVK
                     VTPQITAGVRYSASYNDTIEVTHTTLNNATPSDGSTIAWVVDPSRVTGADYSVTFNPD
                     LTWNLIKAPNDTVVANNANQTGNDAYNVVDGLMVKVVGPDPGINLSIPGPFGDMPGYN
                     GFGITGGQRWVSWPTNWGMETFAGAFGNGFTFFGSDLGPADYVDVEIRFAGIGPSGWP
                     QLDSSAAALMAVNKAAYPDRWQKAVVYWRPNYEVQPTLGDVPFTVWDMESNPPRQLKV
                     AFVEDPRPAYDGAANLIWDMGWGDDAKFGVNGGREYVFILNDTYDEQYTDYLNGTLDG
                     TYNNVMYAGGWGARSETRPFLQDAFEFQIYASNINTVNDVFSFKAPDAANVEKAYLKE
                     DLKKINVFPNPYYGYHSGEMNIFERWVRFTFLPEKCTIKIFDL"
BASE COUNT          584 a          505 c          380 g          552 t
ORIGIN      
        1 acagatcgaa tatcttaatt gtacatttct ccggaaggaa ggtaaaacgt acccaacgtt
       61 caaagatatt catttcaccg ctatgatagc cgtaataggg attcggaaag acgttgattt
      121 tctttaaatc ttccttcaga taggcttttt caacattagc cgcatccgga gccttaaaag
      181 aaaatacatc atttacggta ttaatgttgc tagcatagat ttgaaattcg aaagcatctt
      241 gtaagaaagg acgtgtctca gaccttgctc cccatccgcc ggcatacatt acattgttgt
      301 atgttccatc aagagtacca ttcaaataat ccgtatattg ttcgtcatag gtatcattca
      361 gtataaatac gtattcacgt ccaccattga caccaaattt agcatcatca ccccaaccca
      421 tatcccagat taggtttgcc gcaccatcat atgcaggacg agggtcctca acaaaggcta
      481 cttttaactg tcgaggtgga ttactttcca tatcccaaac cgtaaaagga acatcaccta
      541 aggttggttg cacttcataa ttaggtctcc aatagactac ggctttctgc catctatcgg
      601 gataggcggc tttattgact gccattaacg ccgcggcact cgaatccagc tgaggccagc
      661 ctgacggtcc aatcccggcg aagcgaatct caacatcaac atagtctgcc ggtccgagat
      721 cacttccgaa gaaggtgaat ccgtttccaa aagcgcctgc gaatgtctcc attccccagt
      781 tagtcggcca gctaacccat ctttgacctc cagttatccc aaaaccattg taacccggca
      841 tatcaccaaa cgggccagga atacttaagt taatcccagg atcgggacca actactttca
      901 ccattaaacc atcaaccaca ttataagcat catttccagt ctgatttgca ttatttgcca
      961 ccaccgtatc attcggtgct ttaatgaggt tccaggttaa atcaggatta aaggtaacag
     1021 aataatcggc tccagttact cgagaaggat cgaccaccca ggcaattgta gaaccatcac
     1081 tgggggtcgc attgtttaga gtagtatggg ttacttcgat agtatcatta taagaagcgc
     1141 tatatctgac tcccgccgta atttggggag taaccttgac aatggtaggc gaactcgaaa
     1201 aaatctgagg cgcaccaaat tcaagatagc cttctgctac aacaccaaaa taatattcct
     1261 ttccattaat aagtgggtca ttcacattgc cgactttggg atgcttgttt actaatagcc
     1321 ggataaacct ctgtttcgtg tattccatcg gatcggcatt cttgccaata tcccaggatt
     1381 ccacttttac cgggtcagca cctgatgcat cagaatattg atatacagta taaccataga
     1441 aattataagt cgaatcggtt attactttct cagtattatc aataatgaca gtgtcgccaa
     1501 atgcttgcgc tactattgga tccggtgaat cgtataaact tccatcatca ttatattcag
     1561 aatttccact ccaggttaaa acgacttcat tgggaagttc actgacagtc acttccggcg
     1621 cagctggtgc tttagccaat atgaaattca attcataagc caattgggct aaattatcca
     1681 cttcttttag agttgttaca ctattcgaat tagaagaacc gcgcgcgaca atgactccag
     1741 ctacgatttc ctgcacaccg ggttcgccga attcggctat gccatcacca tcggagtcaa
     1801 cccacggctc catcgtgaac ggtccggtcg tcataaagaa acgcctatca gcaggattag
     1861 aatccaacca accggttcca gtctccggat caccggaaaa catgaacttt gttggcggtt
     1921 tacttggatc tgtaccaacc ccaccttcgg taatcggcga accgtctctc cagaaaccac
     1981 tcatataatt ataaacatcc attccagaac tgggattacc c
//
LOCUS       DAOSMP010000161         1997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_56642_length_1997_cov_14.253347, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000161 DAOSMP010000000
VERSION     DAOSMP010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56642_length_1997_cov_14.253347"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1465
                     /locus_tag="PKX09_10620"
     CDS             <1..1465
                     /locus_tag="PKX09_10620"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOU18313.1"
                     /translation="HLYVIDRSILKNIPEEAAVEILKEEKNQLLIAFIARSEDVRLDL
                     KEEQIPPEDFDTVTQELQNFTSRLAEIEQQLTELAKKAGLIRAYQQELEKHLEFARVR
                     SGMQDAEEITYLQGYCPSDDVSQLMQAASDEGWGILSEDPDESDNPPTLLKMNKIARL
                     IQPVLDFLGTVPGYREYDISQYFLIFFALFVAMIIGDAGYGLIFFLVAIFAHYKLVKQ
                     GKSLPLFLKLLYLLSICTMGWGTITGNWFGSIQIAGLPLLKVLTIPQMATFPELFPDL
                     TVNPQDQVMFICFIIGVTQLGLANIMNFINNLPHLKALGNLGWFGVTVGAFLLVLNLV
                     LGSPLPSFTVPLIGCSVVAIVLFMRQESGTSFLKGITRGLGDGFATFLNVISSFSNII
                     SYIRLFAVGMATVAIATSFNQIAASLLKGFTLPAGMLILIIGHGLNIVMSILSVIVHG
                     IRLNVLEFSSQLGMEWSGYKYEPFREKIKNLTEREIQ"
     gene            1462..1884
                     /locus_tag="PKX09_10625"
     CDS             1462..1884
                     /locus_tag="PKX09_10625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455682.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOU18314.1"
                     /translation="MNIGLIGPAAALGLAALGSVLGCGIAAQTAVGGWKKCFANNKPA
                     PFMLVAFAGAPLTQTIYGFLLMLFINGSDKEPLVQLCAGVFGGLAIGFSAVLQGKVAA
                     RAADALAETGKGTANYFMVIGIVETIAVFVLVFMLLNL"
BASE COUNT          513 a          369 c          491 g          624 t
ORIGIN      
        1 gcatttatat gtaattgatc gtagtatatt gaaaaatata ccggaagaag cggccgtcga
       61 aatattaaaa gaggaaaaaa atcaactact aatagctttt attgcccgat ctgaggatgt
      121 acgtcttgat ctcaaagagg agcaaatccc accggaagat tttgacactg tcacacagga
      181 attacagaat tttacttccc gattagccga aatcgaacag caacttactg agctggctaa
      241 aaaggctggt ttaattcgcg cctatcagca ggaattggaa aagcatctgg aattcgcccg
      301 cgtccgatcg ggaatgcagg atgcagaaga gatcacctat ttgcaaggat attgcccctc
      361 ggatgatgtg tcccaattga tgcaggcggc tagtgacgaa gggtggggta ttctaagtga
      421 agatccggat gagtccgata atccgcccac tttattaaaa atgaataaaa ttgctcggct
      481 aatccaaccg gttttagatt tcctcggcac tgtgcccggt tatcgggaat atgatattag
      541 ccagtatttt ttaattttct ttgcgctatt cgtagcaatg attatcggcg atgccggtta
      601 tggtttgatt tttttcctgg tagctatatt tgcccattat aaactggtaa agcaaggtaa
      661 gtctttgccg ctctttttaa agttgctcta cctgcttagt atatgtacta tgggatgggg
      721 cactataacc ggcaactggt ttggatctat tcagattgcc gggttgccat tattaaaggt
      781 tttgacgata cctcagatgg caacttttcc ggaattattt cccgacctga cggtcaatcc
      841 ccaggatcag gttatgttta tttgttttat cattggtgta acgcaactgg ggctggctaa
      901 tattatgaat tttatcaata acttgccgca tttaaaagct ctgggtaatc tgggatggtt
      961 tggagtaacg gtaggtgcct ttctgttggt attaaattta gttttgggat cgccgttgcc
     1021 ttcatttacg gtgcctctga ttggctgtag tgtagtggca atagttttat ttatgcgcca
     1081 ggagagtggt accagcttct tgaaaggaat cacgcgggga ttaggggacg gatttgcgac
     1141 ttttttgaac gttatcagtt cgttttcaaa tattatttcc tatatacggc tcttcgctgt
     1201 gggtatggcg accgtggcaa ttgctaccag ttttaaccag attgccgcat cgttattgaa
     1261 aggttttact ttaccggctg gaatgttgat attaattatc ggacatggac tgaacatcgt
     1321 tatgagtatt ctatcagtaa ttgtgcatgg tattcggtta aatgtcctcg aattttccag
     1381 ccaattaggt atggaatggt ctggctataa atatgagcca ttcagagaaa aaataaaaaa
     1441 tttaacagaa agggaaatac aatgaatatt ggattgatcg gtccggcagc cgctttagga
     1501 ttagccgctc ttggctcggt tctgggatgc ggtatagccg cccaaactgc cgtcggaggc
     1561 tggaagaaat gcttcgccaa caacaagccg gcgcctttta tgctggttgc ttttgccggt
     1621 gcgccactaa cgcagacaat ttatgggttt cttttgatgc tttttatcaa tggaagtgat
     1681 aaagagccat tggttcagct ctgtgcgggc gtttttgggg gtttggcgat tggtttttcg
     1741 gcagttcttc agggtaaagt tgccgcccgc gccgcggatg cactggctga aaccggcaaa
     1801 ggaacggcta attatttcat ggtaattggt atcgtagaga ctattgccgt gttcgtttta
     1861 gttttcatgt tgctgaactt ataaatcggc gcttagaatc agcgactgag gactgcgctt
     1921 tagtcggctg gtgattatag agtctcagcc agttgttggt agttcgaaga aagtctaaaa
     1981 agttttacta aaataaa
//
LOCUS       DAOSMP010000162         1988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_56980_length_1988_cov_14.540610, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000162 DAOSMP010000000
VERSION     DAOSMP010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56980_length_1988_cov_14.540610"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            567..1700
                     /locus_tag="PKX09_10630"
     CDS             567..1700
                     /locus_tag="PKX09_10630"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOU18315.1"
                     /translation="MKVPLLDLKAQLTTIRSEMLNAITAVLDSTQYIMGPPVADLEEK
                     IADYCGVQFAVGVSSGTDALLVALMALDVKPGDLVITTPYSFFATAGVIARLGARPVF
                     IDIDPRTFNLDPNQLHDWFIKNSRQIKKVKAIIPVHLYGQCADLDPILEIAGKYDIPV
                     IEDAAQAIGASYPSKNGIKKAGSMGKMGCFSFFPTKNLGGIGDGGMVITNDSALAEKI
                     TKLRNHGSHQKYYHSLIGGNFRLDTIQAAALLVKLPHLESWHLARQKHAEYYDKAFND
                     SMIKAPYVAYQRSYHIYNQYVILVPDKRDELRQYLADNEIGTEVYYPVPFHLQECFKY
                     LGYKNGDFPHSEYTAAHSLALPIYPELMEDMQAYVVEKVLKFY"
     gene            1883..>1988
                     /locus_tag="PKX09_10635"
     CDS             1883..>1988
                     /locus_tag="PKX09_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019116824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOU18316.1"
                     /translation="MKYFVHKSSYIDDNVEIGEDTKIWHFCHIQSGAKI"
BASE COUNT          590 a          374 c          419 g          605 t
ORIGIN      
        1 ggatttctac tcttctccga atttgaagcc gacctggcgt aactacttgc ggctacgata
       61 caaggcggta tgtgaaattt tcagatagta gatcggtgga aatttcttaa aattttaccg
      121 aagatttaat tgcatgtaac aaattttaac gatctatatc aattaattcg ttggggactc
      181 aattttttat attggaattg atgctatttt ttttgaaatt actttgtttg agtgatattg
      241 agaaatacag ggaatattgc taactgaaaa tgacatggaa ttgacataaa atcctttcaa
      301 tttattgatt ttggaagttg gtaatcaccg gaagcatata gcgaaatccg tgcttttgtg
      361 agagaagtca cgacagaagt ttagtaatta gctcaaaata tcctgaaatt cgatggattt
      421 atttcttggc ctcgtcgctg aatgtagttg acggggcgga gcctttggaa aggatcggta
      481 actcaggttc ggcgccaaat tctaaccgct agtaaataaa agactaatat ctcattgaaa
      541 ttaaatactc tatctgggaa gtaactatga aagtgccctt actcgattta aaagcccaat
      601 taactaccat acggtctgaa atgctgaatg ccattacggc ggtattggat tcaacccaat
      661 acattatggg tccaccggtg gctgacctcg aggagaagat tgccgattac tgtggtgtgc
      721 aatttgccgt tggcgttagt tccggaactg acgctttgct agttgcttta atggctttgg
      781 atgttaaacc cggcgatctg gtcattacga cgccatattc tttttttgcg acggctggag
      841 ttatcgctcg tttgggagcc cgcccagttt ttatagatat tgatcctcgt actttcaatc
      901 tcgacccaaa tcaactgcac gattggttta tcaaaaattc aaggcaaatt aaaaaggtca
      961 aagccattat tccagttcac ctctatggtc agtgtgccga tctcgatccg attttagaaa
     1021 ttgcgggcaa gtacgatatt ccggtgatcg aggacgccgc ccaggctatt ggagccagtt
     1081 atccctcgaa aaatggcatt aaaaaagccg gttcgatggg taaaatgggt tgcttttcct
     1141 tttttccaac taaaaatctg ggtggaatcg gcgacggcgg aatggttatt accaatgatt
     1201 cagccttagc tgagaaaata accaaattac gtaatcatgg ctcccatcag aaatattacc
     1261 actcactaat tggtggcaat tttaggttgg acacgattca agccgcggcg ttgttggtga
     1321 aattaccgca cctcgaaagc tggcatctcg ctcgccaaaa gcatgccgaa tattatgata
     1381 aagcttttaa cgactctatg ataaaagcgc cttatgtcgc ttatcaaaga tcctaccaca
     1441 tttataatca gtacgtcatt ttggtgccgg ataaaaggga cgaactgcgt caatatttag
     1501 ctgataatga aatcggcact gaagtctatt accccgtacc gtttcatttg caggaatgtt
     1561 ttaagtacct tggttataag aatggtgatt ttccccatag cgaatatact gccgcccatt
     1621 ccttagctct gccaatctac ccggagttga tggaagatat gcaggcttat gtggtggaga
     1681 aagttctcaa gttctattaa gccctttaaa agttaaaata gaaatattcg acaagtttag
     1741 taaagtccaa aatagagaaa gcatctgaaa attcagtaaa agaatagcgg attaaattag
     1801 gaagtagatt agcacgaaaa attattgaat tttccggtta atactattca gtttttatct
     1861 tctttaccat taaaacgtga gtatgaagta tttcgttcac aaatccagtt acattgacga
     1921 taatgtcgag attggcgaag ataccaaaat ctggcacttc tgtcatattc agtccggtgc
     1981 gaaaatcg
//
LOCUS       DAOSMP010000163         1963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_58047_length_1963_cov_5.920335, whole genome shotgun sequence.
ACCESSION   DAOSMP010000163 DAOSMP010000000
VERSION     DAOSMP010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58047_length_1963_cov_5.920335"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..>1963)
                     /locus_tag="PKX09_10640"
     CDS             complement(140..>1963)
                     /locus_tag="PKX09_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOU18317.1"
                     /translation="TDISTTFAISGGTVTDDGGAPVTARGVCWNTSGTPDISDTKTND
                     GESSGAFVSLITGLEPRTTYYLRAYAINNAGIGYGEEVTFKTLDSLEVPTVVTAKVTN
                     IMVKTATCGGNVTAWGGDTVRVRGVCWNTTGNPTITDSKTENGSGLGSFQSILYPLTE
                     NKTYYVRAYATNSKGTGYGAVDSFTTQTPAPDVLKIVAADGSGDYPTVQAALNEVPDY
                     YTGRYIIFVKKGVYHEKLFLDRYKMNVILIGEDRDSTILTYDDYAGKAGGTSNSYSVA
                     IDADDFVAMNITFQNTVKNDGTFSDQQAVALRVNGDRQAYYNCNLLGYQDTYYLWGAC
                     GNRVTGRVYMHKCYIEGSVDFIFGRNIAVFDSCHINVNRNAGTITAASTEAESQFGLV
                     FRDCIITADEIGFNSLPIGNIYLGRPWAAAPRTVFIKCEEPEVLNPGGWLEWNVVPAL
                     YAEYQCTGPGSNTSSRIAISRQLTDEEAQEYTLENIFAKTSYPTIAYDWLPIKPNVNI
                     PGTAIKPDANVPLTFNLGQNYPNPFNPTTTIRYELAQPGKISLTVHNLLGQKVLTVVD
                     TYQPAGYYTVTLDGRQLSSGLYFYRIETEHFRQTRKMMILK"
BASE COUNT          500 a          462 c          473 g          528 t
ORIGIN      
        1 tattgtactc caatttttcc gaataatgca ccgattaata aactggctat tttggggtag
       61 tttaataata aatcggcgct gggaatctaa tcggaacatc aaattatttc agtcatatag
      121 ttatagaatc aatcctttat tacttcaaaa tcatcatttt acgagtctgg cgaaaatgtt
      181 ccgtctcgat ccggtagaaa tataacccgc tggacaattg tcttccatcc agagttaccg
      241 tataatatcc ggctggctgg taagtatcaa cgactgtcag gactttttga cccagcaaat
      301 tatgcactgt caagctaatc ttgcccggtt gggcaagttc atagcggata gtggtcgtcg
      361 gattgaaggg gttcgggtaa ttttggccaa gattaaaagt taacggaaca ttagcatcgg
      421 gtttgatagc agtgcccgga atattgacat tcggtttaat cggtagccaa tcataggcga
      481 tcgtgggata ggaagtcttg gcaaagatgt tttccagagt atattcttga gcttcctcgt
      541 cggtcaattg ccgggaaatg gcgatgcggc tggaggtatt cgagcccgga ccggtacatt
      601 gatattcggc gtagagcgcc ggaacaacat tccattccag ccagccaccc ggattcagga
      661 cttccggttc ttcacattta ataaaaaccg ttcgtggggc cgccgcccag gggcgaccga
      721 gatagatgtt cccgatcggc aggctattaa acccgatttc gtcggcggta atgatacaat
      781 ctctgaaaac caagccgaat tgcgattccg cttccgtact ggcggcggtt attgtgccgg
      841 cgttgcgatt gacattaatg tggcaagaat cgaagacggc aatgttgcgt ccaaagataa
      901 aatccaccga gccttcgata tagcatttgt gcatataaac gcgaccggta acgcgattac
      961 cacaggcgcc ccagagataa taagtatcct ggtaacctaa caaattacaa ttataataag
     1021 cctgccggtc gccattgact ctcagagcca ctgcttgttg atcgctaaac gtgccgtcgt
     1081 tttttactgt attctggaaa gtgatattca tcgccacaaa atcgtcggca tcaattgcga
     1141 cactataaga attcgacgtt ccgccggctt taccggcata atcgtcgtaa gtcaaaatgg
     1201 tgctgtcgcg gtcttcacct atgagaatga cgttcatttt gtagcgatcc aggaacaatt
     1261 tttcgtgata aacgcctttt ttaacaaaga taatgtagcg accggtgtaa taatcgggta
     1321 cctcattaag agccgcctgc acggttgggt aatccccact gccgtccgcg gctacgattt
     1381 tcagcacatc cggagctgga gtttgcgttg tgaaagaatc cactgctcca tagccagtgc
     1441 ctttactatt agtggcataa gcgcgtacat aataagtttt gttttcagtc agcgggtaaa
     1501 gaatgctctg aaaagaaccc agcccgctac cgttctcggt tttgctgtcg gtaatagtag
     1561 gattgccggt tgtattccag caaacgccgc ggacgcgtac cgtatcgcca ccccaggcag
     1621 ttacgtttcc gccacaggtg gcagttttta ccattatatt ggttacttta gccgtcacaa
     1681 ccgtgggtac ttccagtgaa tccagtgtct taaaagtaac ttcttcgcca taacctatac
     1741 cggcgttatt gatggcatac gctcggaggt aataagtcgt gcggggttcc aatccggtga
     1801 tgaggctgac aaaagcgcca ctgctttcac catcattagt tttggtgtcg gaaatgtccg
     1861 gcgtgccgct cgtattccag caaacgccgc gggcggtcac cggcgcgccg ccgtcatcgg
     1921 tgacagtacc gccactgata gcaaaggttg tcgaaatatc agt
//
LOCUS       DAOSMP010000164         1960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_58182_length_1960_cov_9.124934, whole genome shotgun sequence.
ACCESSION   DAOSMP010000164 DAOSMP010000000
VERSION     DAOSMP010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58182_length_1960_cov_9.124934"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="PKX09_10645"
     CDS             <1..1064
                     /locus_tag="PKX09_10645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU18318.1"
                     /translation="NTYKTGFSIPANGGNLSTGAYDVYSLYASSIDARMNWWASNANR
                     YGNFLIAPTADDGGLLKTVSQYSIENGQDQIIFQETMSQASQLISDSLFNEAIAVYRM
                     IISDYPHCLESAFALNGIVYSYYCLQHPEKILSELESLLLTNKGNLVEIFIKDFISME
                     YIKNERFIEASNLLDELLIDYEKYEMDEHAAYTLLNKTYLQQYLKNNLSIGKKGETTA
                     QFNSVECLALLFSKYPNSIAASLAKEEFVNDFIIDVPLPEKAEVLLADHRIYPNPFNP
                     KTTISFDLPEQTVVEIIVYDLMGREVWKSAKTNYYAGTYSVIWNGVNHSGQPVGTGIY
                     LVRLNSNKYTATQKVLLMK"
     gene            1079..1510
                     /locus_tag="PKX09_10650"
     CDS             1079..1510
                     /locus_tag="PKX09_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOU18319.1"
                     /translation="MDEYTQFMELMGDKVTQVIEKPRRLYFVVSNENIQEIARYLFVT
                     LGCRLSTATAQETYHGLEILYHFSHDASGCYYCPRVVLTDKKNPKVNTITDIVTGALW
                     IEREIFDLWGIEFVGHPRMEQLLTLNSPENPDKPLRFGRSL"
     gene            1507..>1960
                     /locus_tag="PKX09_10655"
     CDS             1507..>1960
                     /locus_tag="PKX09_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOU18320.1"
                     /translation="MIENKIPLGPFHPLLEEAEYFELKVDGETVVDIDMEIGWMHRGH
                     EFISESKTFTQSIYLVERICGICSTSHPLACVHAIEDIDNIEIPLRARYIRTLIGELE
                     RIHSHLLWLGLAGHFIGFDTLWMWAWKYREPILEMFEAITGNRNHYGMM"
BASE COUNT          583 a          380 c          429 g          568 t
ORIGIN      
        1 aaaacactta taaaacaggc ttttcgattc cagctaatgg gggaaaccta agtaccggag
       61 cttatgatgt ctatagtctg tatgcatcat ctattgatgc caggatgaat tggtgggcgt
      121 ccaacgctaa tcgatatggt aattttctaa ttgcacctac ggcagatgat ggcggcttac
      181 ttaaaacggt atctcaatat tctatagaaa acggacaaga tcagatcata tttcaggaaa
      241 ctatgtctca agcgagccaa ttgatttcag atagtctatt taacgaagca atagccgtct
      301 atagaatgat aatttcggat tacccacatt gcttagagtc agcatttgct ttaaatggca
      361 tcgtttatag ttattattgt ctgcaacatc ccgaaaaaat tctatctgag ttggaatcat
      421 tgcttcttac taataaaggt aacttggttg aaatatttat aaaagatttt atctcaatgg
      481 aatatattaa aaacgaacga tttattgagg cgtcaaattt acttgatgaa ttgttgattg
      541 attatgaaaa atacgaaatg gatgaacatg ccgcatatac acttttgaat aaaacttact
      601 tacaacagta cctaaaaaat aatttgtcta tagggaaaaa aggagaaacg acggcccaat
      661 ttaactcagt agaatgtctg gcattactat tttcaaaata tccaaattct atagcagctt
      721 cattggctaa agaggagttt gttaatgatt ttattattga tgtaccttta ccagaaaaag
      781 cggaggtatt attagccgat catcggatct atcctaatcc ctttaatcct aaaacgacta
      841 tttcattcga tcttcccgag caaactgtag ttgagattat tgtttacgac ctgatggggc
      901 gcgaggtctg gaaatcggcg aagactaatt attatgccgg aacctattcg gttatctgga
      961 atggcgtcaa tcattccggg caaccggtcg gaaccgggat atatctcgtc cggctgaatt
     1021 ccaacaaata taccgcaacg caaaaagtgt tactgatgaa atgaaaatgg agatattaat
     1081 ggatgaatac acgcaattta tggaattaat gggcgacaag gttacgcagg taatcgaaaa
     1141 accgcgccgg ctctattttg tggtatccaa tgaaaatata caggaaattg cccggtattt
     1201 gttcgttacg cttggctgtc ggctctcgac tgctactgct caagaaacct atcacggtct
     1261 cgaaatcctg tatcattttt cgcacgacgc cagcggttgc tattattgcc cacgggtggt
     1321 tttaaccgac aagaagaatc caaaggttaa tactatcacc gatatcgtga ccggtgcact
     1381 gtggatcgag cgcgagattt tcgacctgtg gggcatcgaa ttcgtcggtc atccgcgtat
     1441 ggaacaactc ctgacgttga atagtccgga aaatccggat aaaccgttgc gcttcgggag
     1501 gtcgttatga tcgaaaacaa gattccactt ggtccatttc atccgctatt ggaagaagcc
     1561 gagtacttcg aactgaaagt ggacggtgag acggtggttg atattgatat ggaaatcggc
     1621 tggatgcacc gcggacatga gttcatcagc gaatccaaga cttttaccca atctatttat
     1681 ctagttgaaa gaatttgtgg catctgctca acttcgcatc cgcttgcctg cgtacatgct
     1741 atcgaggaca ttgataatat cgaaattcca ctgcgagcgc gctatatccg caccttaatt
     1801 ggcgaactgg aacggattca tagccattta ttgtggttgg ggctggcagg gcattttatc
     1861 ggatttgata cgctttggat gtgggcttgg aaataccgtg agccgattct ggaaatgttt
     1921 gaagccatta ccggcaaccg caatcattac ggtatgatgc
//
LOCUS       DAOSMP010000165         1939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_59053_length_1939_cov_19.360934, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000165 DAOSMP010000000
VERSION     DAOSMP010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59053_length_1939_cov_19.360934"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /gene="glgC"
                     /locus_tag="PKX09_10660"
     CDS             complement(<1..1045)
                     /gene="glgC"
                     /locus_tag="PKX09_10660"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOU18321.1"
                     /translation="MKIIDVLAIILGGGKGTRLYPLTKYRAKPAVPFGGKFRIIDVPI
                     SNCLNSGINKIYILTQFNTHSLHRHIYSTYSLGPFSNGFVDILAAQQTMENTDWYQGT
                     ADAVRKNLPFINEIKAAYTMILSGDHLYRMDYRLFLEHHIKNKAEITVAVKPVSRPDA
                     GHFGILQTDEKCRIVQFVEKPNSELLDKLKSPGLPDETPFLASMGIYIFATETLHKIL
                     GQNPSDDFGKNIIPASIPTLNVCAYQFDDYWRDIGTIESFFTANLELARFQPPFSLYD
                     EHAPIYTHARSLPASRLENCSLTQTLLADGCHIGESKISESVVGIRTIIQKGTTIEKC
                     VIMGADYFEQVQPE"
     gene            complement(1042..1647)
                     /locus_tag="PKX09_10665"
     CDS             complement(1042..1647)
                     /locus_tag="PKX09_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18322.1"
                     /translation="MRLTKNQQNALRVARLISVILHPLVIAPLTYALIIAHTSEINRT
                     QKLLYLGLVLIAIIFIPLISVLYLKKRGETTSLDVPERDKRISPFLVCIAGYFIVWIL
                     LKVSGAPRPISLLMWCYGFNSIIATIITKYWKISVHGMALGGPIAALGFVISPTFYWG
                     ILIAPLIVYSRVKLKAHTIAQVITGFLLGFLLTLIQFEIFL"
BASE COUNT          628 a          372 c          410 g          529 t
ORIGIN      
        1 cttctggctg aacctgttcg aagtaatcag ctcccataat gacgcatttc tcaatggtgg
       61 tacctttctg gataatcgtt ctaataccga caacagattc gctaatcttg ctctcaccga
      121 tatgacagcc gtctgccagc agagtttgag tcagtgaaca attctctaat cgggaagccg
      181 gtaaggagcg ggcatgagta taaataggag cgtgttcatc gtataaagaa aaaggcggtt
      241 ggaatctagc aagttcaaga ttggcggtaa aaaacgattc aatggtacca atatccctcc
      301 aatagtcatc aaattgatag gcacagacat taagcgtagg aattgaagcg ggtatgatat
      361 ttttaccgaa atcgtcgctc ggattttgtc cgagaatttt atgaagggtt tccgtggcaa
      421 aaatatagat gcccatcgaa gctagaaagg gtgtctcgtc tggtaagccg ggagatttta
      481 acttatcaag aagctccgaa ttgggttttt ccacaaattg aactatacga catttttcat
      541 cggtctgcaa aatgccgaaa tgtccggcat ctggtcgtga aaccggcttg accgctaccg
      601 taatttccgc cttattcttg atatgatgtt ccagaaacaa acggtaatcc atacggtaga
      661 ggtggtcacc agaaagaatc atagtatagg cggctttgat ttcattgatg aacggaaggt
      721 ttttccgaac agcatcagca gttccctgat accagtcagt gttttccata gtctgttgag
      781 ccgccaaaat atcaacaaaa ccattactaa agggacctaa gctataagtg ctgtaaatat
      841 gacgatgaag cgaatgagta ttgaattggg taagaatgta gattttattg attccgctat
      901 tgaggcaatt gctaatcggt acatcaataa tgcggaattt gccaccgaaa ggaacagccg
      961 gcttggctcg atatttggtt aaaggataaa gccgtgtgcc tttgcccccg ccgagaataa
     1021 ttgccaaaac gtcaatgatt ttcataaaaa aatctcgaat tggattaaag ttaataaaaa
     1081 tcctaataga aaacccgtaa ttacttgagc gatagtgtga gccttcaact tcactcgcga
     1141 atagacaatc aatggagcga tcaaaatacc ccagtaaaaa gtcggtgaaa ttacaaagcc
     1201 taaagccgct atgggaccgc ccaatgccat accatgcaca cttattttcc agtatttggt
     1261 gataatagtg gcaataattg aattaaatcc atagcaccac attagtaaag aaatagggcg
     1321 cggtgcccca gacactttta acaaaatcca tacaataaaa taacctgcga tacagactaa
     1381 aaagggacta atccgtttat ctctttcagg aacgtccagg ctggtcgtct cccctctttt
     1441 ttttaggtat aagacagaaa tgagtgggat aaaaatgata gcgataagca ctaaccctaa
     1501 atatagcaat ttttgagttc gattgatttc ggaagtatgc gcaataatca aagcataggt
     1561 cagcggcgct atgacgagtg gatgcagaat taccgagatt aaacgggcta ctcgaagtgc
     1621 attttgttga tttttagtca atcgcataat gactatagtc tgggatagtt gtgaatcaat
     1681 aatctattat atttcattcc gtaaaattat acgttctttt tatatcaatt tcaacaaata
     1741 aatcacgaga aaaaacataa agtttgtaaa agcgctcaat taattgacta tttgattatt
     1801 actattgaaa gcgtggtgtc ctaaccaccc tgcccgacta atggtcgcat gaccaatagg
     1861 cgggtagacg atagcgcctt taaaaagaaa taaaggctaa agaaaagact tggaacgatt
     1921 ggtagcgcta cggagaatt
//
LOCUS       DAOSMP010000166         1920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_59904_length_1920_cov_15.130295, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000166 DAOSMP010000000
VERSION     DAOSMP010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59904_length_1920_cov_15.130295"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="PKX09_10670"
     CDS             complement(<1..1052)
                     /locus_tag="PKX09_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HOU18323.1"
                     /translation="MFFSTQILIAQVVYSNPPHPTERDSIIIYFDAAKGDGGLKDFTG
                     DVYAHTGVITNLSATAKDWRYVITPWPGEGGTANTSKNKLERISTNLYKLTIGFPRTF
                     YISHKNGATIPDGEKILKLAFVFRNADGSKSGRDVGGADIFQNLFEVGLTVIVSEPVI
                     DLSLSDPRRSPVFTNHGDTVDIKIITVALNTKISAIKLFVNHELINQVTDTVMSFDFV
                     AEEYNYGMNNLMAIGTDTSGFSDTASFAIMINPPLTSISRPEGIVDGINVLNNNSITF
                     SLQAPFKDFVYLIGDFNDWLVDTSYFMHCEDNGGTDVHWWLTIDDLDSQTEYAFQYFV
                     DGEIRLADPYSEKILDP"
     gene            complement(1099..>1920)
                     /locus_tag="PKX09_10675"
     CDS             complement(1099..>1920)
                     /locus_tag="PKX09_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU18324.1"
                     /translation="LEYWVKEFDIDGYRLDVAELVPDDFWKIAAHRLQKIKPDILMLA
                     EGDHPRLYLNGFHLTYGWNTRRAFYQIIKNDYPAEFLAQTLRKEYYRYPKNALKMRFT
                     ENHDEARTTALFTAEQTLVLTFLIFTLPGVPMIYAGQEIGASEKPSLFEKDTINWRQF
                     DKSLNELITKMNELRRNISALSSDTYQVINTSDTESIFAFSRYNAQEVVLIVTNLKSK
                     SQMATIDLAPISAKIPNTEPELLVGTGAFLLENNTLYLPCYGYDYYLFRFETKND"
BASE COUNT          572 a          407 c          384 g          557 t
ORIGIN      
        1 ggatccagaa ttttttcgga atagggatcc gccaggcgga tttcaccatc aacaaaatat
       61 tggaaagcat attcggtctg tgaatcgaga tcgtcaatgg tcagccacca gtgcacgtcg
      121 gttccgccgt tgtcttcgca atgcataaaa tagctcgtgt caaccagcca atcattaaag
      181 tcgccaatca gatagacgaa atccttaaaa ggcgcttgta gtgaaaaggt aatgctgttg
      241 ttatttagca cattgatacc gtcaacaata ccttcgggtc gagaaataga cgtcagcggt
      301 ggatttatca taatcgcaaa actggcggta tcgctgaatc cactcgtgtc agtaccaatc
      361 gccattagat tattcattcc ataattatat tcctctgcca caaagtcaaa actcatcaca
      421 gtgtcagtga cctgattgat taactcatga ttaacaaata attttatagc actaatctta
      481 gtattgagag ctaccgtgat aatttttata tcaaccgtat ctccatgatt cgtaaaaacg
      541 ggtgagcgac gcggatcgga taggcttaaa tcaataacgg gttcactgac aattaccgtc
      601 aatcctactt caaataagtt ttggaaaatg tcggctccac caacatccct cccactctta
      661 ctgccatcag cattgcggaa gacgaaggct aatttgagaa ttttttcgcc atcgggaata
      721 gttgcgccat ttttatgaga aatataaaaa gtacgcggga agccgatagt cagtttgtat
      781 aagttagttg aaattctctc gagtttattt ttcgaagtat tagctgtacc accttcccca
      841 ggccagggag tgatgacata gcgccagtct ttggcggtcg cgcttaaatt agtaataacg
      901 ccggtatgcg cataaacatc gcctgtgaaa tctttcaaac cgccgtcgcc tttagcggca
      961 tcgaagtaaa ttatgatcga atcccgttcg gtcggatgag gcggattaga gtaaaccacc
     1021 tgagcgatta gaatttgggt actaaaaaac atcaggcata gcgcgcctac tttgtataaa
     1081 attttgttaa ggtggttatt aatcattttt ggtctcaaac cggaataaat aataatcata
     1141 tccgtaacac ggcaaatata atgtattatt ttccagcaaa aatgctccag ttccgaccaa
     1201 taattccggt tcagtattgg gaattttcgc tgaaatcggc gccaaatcaa tcgtcgccat
     1261 ctgagattta cttttcaaat tggtaacaat aagtacaact tcttgagcat tatatcgaga
     1321 aaatgcgaag atgctctccg tgtcggaagt atttataacc tggtatgtgt ccgatgatag
     1381 agcagaaatg ttgcgccgca gttcattcat tttcgtgatt aattcattta aacttttgtc
     1441 aaactgacgc cagttgatag tatctttctc aaataaagat ggtttttcac tagcgccgat
     1501 ttcctgcccg gcataaatca tcgggacgcc cggtaaggta aatatcagga aggtcaagac
     1561 cagagtttgt tctgccgtaa ataaagccgt tgttcgcgct tcatcatgat tttcggtaaa
     1621 acgcatttta agtgcattct tagggtaacg ataatactct ttcctcagag tttgagctaa
     1681 aaattcagcc ggataatcat tcttaataat ctgataaaaa gcccgccttg tattccaacc
     1741 ataggtcaaa tgaaaaccat taagatacaa acgaggatga tcgccttccg ccagcattaa
     1801 aatatccggt ttgatttttt ggaggcggtg cgcggctatt ttccagaaat catccggtac
     1861 cagttcagcg acatcgagcc ggtatccatc tatatcaaac tctttgaccc aatattctaa
//
LOCUS       DAOSMP010000167         1911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_60280_length_1911_cov_11.192349, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000167 DAOSMP010000000
VERSION     DAOSMP010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60280_length_1911_cov_11.192349"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /gene="ltaE"
                     /locus_tag="PKX09_10680"
     CDS             <1..1010
                     /gene="ltaE"
                     /locus_tag="PKX09_10680"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HOU18325.1"
                     /translation="TVTKPSPEMRAAMASAEVGDDVYGEDPTVNRLQERVAALLGKEA
                     ALFVPSGTMSNQIAIKCLTQPGDEIICEYESHIYTYEASGPAFNSLVMPRPVKGHYGV
                     MAIEDVARAIRPDNIQTGQTSLIEVENTHNRAGGTIYPLAEIEKLAEIANEHRIPMHL
                     DGARLMNAAVSTGIAPAEYAKYFDSVSLCLSKGLGAPIGSVLSGSRNFIAKALRVRKI
                     FGGAMRQVGILAAAGLYALDHNVQRLKIDHENAWLLAQGLSEIEGLYIDKQQVQTNMV
                     IIFARSPRWTTEKIVQQLLEKGIGMNAVDQERIRAVTHLDVSRDQILETIEIFKKIME
                     A"
     gene            1014..>1911
                     /gene="trkA"
                     /locus_tag="PKX09_10685"
     CDS             1014..>1911
                     /gene="trkA"
                     /locus_tag="PKX09_10685"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOU18326.1"
                     /translation="MKIIIIGAGEVGYYLAKRLISEDHNVTIIEMDSERHRRAAETLD
                     AIVIHNSGSSPRVLENAGVKSTDILLAVTGSDDTNILSCIIAKKLGVARCLARVHSEE
                     FSFSKSVITGQDIGIDLLIHPERTAAEATIRLVEQSSASQLVTFEDDRLQIISLLVKE
                     DSPVVNLTLKEVATQHDQFSFLCLAIHREGKTIIPRGKNVYRSGDTAYFIAKEEDVPR
                     LTALLGYPKTEQQHIMILGAGRLGRMVAAVLSREMNVKLIEQDRDIAEEVAVNLPDTL
                     VLHGDGTDIDFLSSERIEEMDCF"
BASE COUNT          542 a          352 c          487 g          530 t
ORIGIN      
        1 atacagttac caaaccttct cctgaaatgc gggcggcgat ggcgagcgcc gaagttggtg
       61 acgatgttta tggcgaagac ccgacggtaa atcgtctgca ggagagggta gcggctcttc
      121 tgggcaagga agccgcttta tttgtcccga gtgggacaat gtcgaaccag attgcgatta
      181 aatgtcttac tcaaccgggc gatgaaatta tctgcgagta tgaaagtcac atttacacct
      241 atgaagctag cggtccagca ttcaattcgt tggtaatgcc gcgtccggtg aagggacatt
      301 atggagttat ggcaatcgag gatgttgcca gagctatacg accagacaat attcagactg
      361 ggcagacgag tttaatcgag gtcgaaaata ctcataatcg tgctggtggt acgatttatc
      421 cattggcaga aattgagaaa ctggctgaaa ttgccaatga acatcgtatt ccgatgcact
      481 tagacggtgc gcgcctgatg aatgcggcgg tgagcaccgg cattgcccct gccgaatatg
      541 ctaaatattt cgattcggtg agtctatgtt tgtcaaaagg tctgggcgcg ccgatcggat
      601 cggttttaag tggttcgcgg aactttattg ctaaagccct gcgagttagg aaaatttttg
      661 gtggagcaat gcgacaagtt gggattttgg ccgcggctgg tttatatgcc ctcgatcata
      721 atgttcagcg cctgaaaatt gaccacgaga atgcctggct attggcacaa ggactgtcag
      781 aaatcgaagg actgtatatc gataaacaac aagtgcaaac caatatggtg ataatttttg
      841 cccggagtcc gcgctggacg actgaaaaaa ttgttcagca attattagaa aaaggtattg
      901 ggatgaatgc cgttgatcag gaaagaattc gcgctgtaac tcatctcgat gttagtcgtg
      961 atcaaatact tgaaacaatt gaaatattta agaaaattat ggaagcttaa gcaatgaaaa
     1021 taattataat tggcgccggt gaagttggtt attatttagc caagcgtctt attagcgaag
     1081 atcataacgt aactataatc gaaatggatt cggaacgtca tcgtcgagcg gcagaaacac
     1141 ttgatgccat tgtcattcac aatagtggtt ccagtccgcg agttttggag aatgctggag
     1201 taaaatctac cgatattctt ttagcggtta cgggaagtga cgacaccaat attctatcct
     1261 gtataatagc caaaaagttg ggagtagccc gttgtttagc gcgtgttcat agtgaagaat
     1321 tcagtttttc aaagtcagtt attactggtc aagatattgg tattgattta ttaattcatc
     1381 cggaacggac ggcggccgaa gctactatcc ggctggtgga gcagtcctcg gcatctcaat
     1441 tggtaacttt tgaagatgac cgtttgcaga ttatttcatt gttagtaaaa gaagatagtc
     1501 cggtagttaa cttaactttg aaagaagttg ctactcagca tgaccagttt tcatttttat
     1561 gtttggcgat tcatcgggaa ggtaagacca tcataccgcg cggaaaaaat gtttacagaa
     1621 gtggtgatac ggcttacttc attgccaaag aagaagatgt tccgcgttta acggcactgc
     1681 tcggttatcc caagaccgaa caacagcata ttatgattct gggcgctggt cggctcggga
     1741 gaatggtcgc agctgtttta tcgcgtgaaa tgaatgtcaa gctgattgag caagatcgtg
     1801 atatagctga agaagtagcc gttaatttac ctgatacatt agtcttgcat ggtgatggta
     1861 ccgacattga ttttcttagt tcagaaagaa tagaagaaat ggattgtttt g
//
LOCUS       DAOSMP010000168         1911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_60283_length_1911_cov_9.872845, whole genome shotgun sequence.
ACCESSION   DAOSMP010000168 DAOSMP010000000
VERSION     DAOSMP010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60283_length_1911_cov_9.872845"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /gene="ltaE"
                     /locus_tag="PKX09_10690"
     CDS             <1..1010
                     /gene="ltaE"
                     /locus_tag="PKX09_10690"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HOU18327.1"
                     /translation="TVTKPSPEMRAAMASAEVGDDVYGEDPTINRLQERVAALLGKEA
                     ALFVPSGTMSNQIAIKCLTQPGDEIICEYESHIYTYEASGPAFNSLVMPRPVKGYYGV
                     MAIEDVVKAIRPDNIHSGPTGLIEVENTHNRAGGTIYPLVEIEKLAEIANEHRIPMHL
                     DGARLMNAAVSTGIAPAEYAKYFDSVSLCLSKGLGAPIGSVLSGSRSFIAKALRVRKI
                     FGGAMRQVGILASAGLYALDHNVQRLKIDHENAWLLAQGLSEIEGLYIDKQQVQTNMV
                     IIFARSPRWTTEKIVQQLLEKGIGMNAVDQERIRAVTHLDVSRDQILETIEIFKKIME
                     A"
     gene            1014..>1911
                     /gene="trkA"
                     /locus_tag="PKX09_10695"
     CDS             1014..>1911
                     /gene="trkA"
                     /locus_tag="PKX09_10695"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOU18328.1"
                     /translation="MKIIIIGAGEVGYYLAKRLISEDHNVTIIEMDSERHRRAAETLD
                     AIVIHDSGSSPRVLENAGVKSTDILLAVTGSDDTNILACIIAKKMGVARCLARVHSEE
                     FSFSKSAITGQDIGIDLLIHPERTAAEATIRLVEQSSASELVTFEDDRLQIISLIVKE
                     DSPVVNLTLKEVATQHDQFSFLCLAIHREGKTIIPRGKNVYRSGDTAYFIAQEEDIPR
                     LTALLGYPKTEQQHIMILGAGRLGRMVAAVLSREMNVKLIEQDRDIAEEVAVNLPDTL
                     VLHGDGTDIDFLSSERIEEMDCF"
BASE COUNT          536 a          356 c          491 g          528 t
ORIGIN      
        1 atacagttac caaaccttct cctgaaatgc gggcggcgat ggcgagcgcc gaagtcggcg
       61 acgatgttta tggcgaagac ccgacgataa atcgtctgca ggaaagggta gcggctcttc
      121 tgggcaagga agccgcatta tttgttccga gcgggacaat gtcgaaccag attgcgatta
      181 aatgtctcac tcagcccggt gatgaaatta tctgcgagta tgaaagtcat atatacacct
      241 atgaagccag cggtccagca ttcaattcgt tggtaatgcc gcgcccggtg aagggttact
      301 acggagttat ggcgatcgag gatgtagtca aggctattcg acctgataat attcattctg
      361 gtccgacggg tttaatcgag gttgaaaata ctcataatcg tgccggtggt acgatttatc
      421 cattggtgga aattgagaaa ctggctgaaa ttgccaatga acatcgtatt ccaatgcact
      481 tagacggtgc gcgcctgatg aatgcggcgg tgagcacagg cattgcccct gccgaatatg
      541 ctaaatattt cgattcggta agtctatgtt tatcaaaagg tctgggcgcg ccgatcggat
      601 cggttttaag tggttcacgg agctttattg ccaaagcact gcgagttagg aaaattttcg
      661 gtggagcaat gcgacaagtt gggattttgg cttcggctgg tttatatgcc ctcgaccata
      721 atgtccagcg cctgaaaatt gaccacgaga atgcctggct attggcacaa ggactgtcag
      781 aaatcgaagg actgtatatc gataaacaac aagtgcaaac caatatggtg ataatttttg
      841 cccggagtcc gcgctggacg actgaaaaaa ttgttcagca attattagaa aaaggtattg
      901 ggatgaatgc cgttgatcag gaaagaattc gcgctgtaac tcatctcgat gttagtcgtg
      961 atcaaatact tgaaacaatt gaaatattta agaaaattat ggaagcttaa gcaatgaaaa
     1021 taattataat tggcgccggt gaagttggtt attatttagc caagcgtctt attagcgaag
     1081 atcataacgt aactataatc gaaatggatt cggaacgtca tcgtcgagcg gcagaaacac
     1141 ttgatgccat tgtcattcac gatagtggtt ccagtccgcg agttttggag aatgccgggg
     1201 taaaatctac cgatattctt ttagcggtta cgggaagtga cgataccaat attctggctt
     1261 gtattatcgc aaaaaaaatg ggagtagctc gctgtttggc gcgtgttcat agtgaagaat
     1321 tcagtttttc aaagtcggct attaccggtc aggatatcgg tattgactta ttaattcatc
     1381 cggaacggac ggcggccgag gctactatcc ggctggtaga gcagtcttcg gcatctgaat
     1441 tggtgacttt tgaagatgat cgtttgcaga ttatttcatt gatagtaaaa gaagatagtc
     1501 cggtagttaa cttaactttg aaagaagttg ccactcagca tgaccagttt tcgtttttat
     1561 gtttggcgat tcatcgggaa ggtaagacca tcattccgcg cgggaaaaat gtttacagaa
     1621 gtggtgatac ggcttacttt attgctcaag aagaagatat tccgcgttta acggcactgc
     1681 tcggttatcc gaagaccgaa caacagcata ttatgattct gggtgctggt cggcttggga
     1741 gaatggtcgc agctgtttta tcgcgtgaaa tgaatgtcaa actgattgag caagatcgcg
     1801 acatagctga agaagtagcc gttaatttac ctgatacatt agtcttgcat ggtgacggta
     1861 ccgacattga ttttcttagt tcagaaagaa tagaagaaat ggattgtttt g
//
LOCUS       DAOSMP010000169         1872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_62101_length_1872_cov_14.835993, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000169 DAOSMP010000000
VERSION     DAOSMP010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62101_length_1872_cov_14.835993"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PKX09_10700"
     CDS             <1..344
                     /locus_tag="PKX09_10700"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOU18329.1"
                     /translation="DVYNFGQMKRDFTYIDDIVAGLRSAIDKPFPYEIFNLGNHRSEN
                     LMDFIHLIEEYSGQKAQINFQPIQPGDVPETYADISTATEKLGFVPRTQIREGIKLFL
                     DWYFEYYHIKK"
     gene            350..1333
                     /gene="rfaE1"
                     /locus_tag="PKX09_10705"
     CDS             350..1333
                     /gene="rfaE1"
                     /locus_tag="PKX09_10705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HOU18330.1"
                     /translation="MLETLSTDLNNLDKIFQEIQNLHILVVGDLILDQYIWGKVERIS
                     PEAPVPIITVSRKENRLGGAGNVAANLSALECPVTLAGVCASDQAGRDLQTLCHQKKI
                     NSIIATLPERRTSCKARIIAQQQQLLRIDEETIEPVAKKETEQLLKNITALANQFDAV
                     ILSDYAKGLVTEDFIQSILEIFKDKPVLVDPKGFNYQKYRGATALKPNFREFCMAVRH
                     PELTLAKIEPLARQLIDELNLKGLIVTLGEQGVFVLDTDQKAALIPTKAREVFDVSGA
                     GDTFIAVFTAVLMVTNDWKIAAVVANLASGIAVGKIGTATITTAEILAEDY"
     gene            complement(1382..>1872)
                     /locus_tag="PKX09_10710"
     CDS             complement(1382..>1872)
                     /locus_tag="PKX09_10710"
                     /inference="COORDINATES: protein motif:HMM:NF020752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOU18331.1"
                     /translation="RQKLNFSFEKLEESGKIITRLRDFKKSLEGEFPTGNASAMEIVE
                     KGEADFGSALDDDLNISNALAAVFKLLHEINLYRQKTPLTQADIELIRQSLERIDSVL
                     NILSEPHEVLSEEERKLIDARNQARRERNWAEADRLRNLLLERGIILEDTPKGTTWKR
                     KI"
BASE COUNT          506 a          440 c          409 g          517 t
ORIGIN      
        1 ttgacgtcta taattttggt cagatgaagc gcgattttac ttatattgat gatattgtcg
       61 ctggattgag gtcggcaatt gataaacctt ttccatacga aatttttaat ctcggcaatc
      121 atcgcagtga aaacttgatg gatttcattc atttaatcga agaatactcg gggcaaaaag
      181 ctcagataaa ttttcagcca attcaacccg gcgatgtacc ggaaacctat gccgacatct
      241 ccaccgctac ggaaaaactc ggcttcgtgc cgcgcacgca gatccgagaa ggcatcaaat
      301 tattcctcga ttggtatttc gaatattatc atattaaaaa gtagtaaata tgcttgaaac
      361 actatctacc gatttgaaca acctggataa aattttccag gaaatccaaa acctgcatat
      421 tctggtggtt ggcgacctga ttctcgacca atatatctgg ggtaaggtcg aacgcatttc
      481 accggaagcg cccgtgccta ttataactgt cagccgtaaa gaaaatcgcc tcggcggagc
      541 cggcaacgtc gccgccaact tgagcgcttt ggaatgtccg gtaactttag ctggtgtgtg
      601 cgcttccgat caagctgggc gggatttaca gactttatgt caccagaaaa aaattaactc
      661 aattatcgcc acattgcccg aacggcgtac atcttgtaaa gcgcgaatca tcgcgcaaca
      721 gcaacaattg ctccgcattg acgaagaaac catcgaaccg gtagccaaga aagagaccga
      781 gcaacttctc aaaaatatta cggctttagc caatcaattc gatgccgtaa ttctttccga
      841 ttacgccaaa ggtctggtca cggaagactt tatccaatcc attctggaaa tattcaagga
      901 taaaccagta ctggtcgatc ccaagggttt caactatcaa aaatatcgtg gcgcgaccgc
      961 cctcaagccc aatttccgtg aattttgtat ggcggtgcgt catcccgaat tgaccctcgc
     1021 taaaatcgaa ccgcttgctc gacaattgat tgatgaactt aacctgaagg gtttgatcgt
     1081 tactctgggc gaacaagggg ttttcgtact cgatactgac cagaaagccg ctctaattcc
     1141 cactaaggcg cgcgaagtct tcgatgtcag cggtgccggt gatactttta tcgccgtttt
     1201 caccgccgtt ctaatggtta ctaatgattg gaaaatagca gctgtagtag ctaatttagc
     1261 atctggtatt gccgtcggga aaatcggcac ggctaccatc acaaccgctg aaattctcgc
     1321 tgaagattat tagctattca ccactaaggt gtgtagataa atctattgat tttttgaaga
     1381 gtcagatttt gcgtttccag gtggtgcctt tgggagtgtc ttcgagaata atgcctcgtt
     1441 caaggagaag gtttcgcaag cgatcggctt ccgcccaatt acgttcccgc cgggcttgat
     1501 ttctggcatc aattaacttc cgctcttcct cagaaagtac ctcgtgcggt tcagacagga
     1561 tgttcagaac cgaatcaatt cgttccagag attgtcggat taattctata tctgcctgag
     1621 ttagcggagt tttttggcga tataaattga tttcgtgcaa taacttaaaa acagccgcaa
     1681 gggcattgga gatattcaga tcatcatcga gcgccgaccc gaagtcggct tcacctttct
     1741 cgacgatttc catagcgctg gcatttcccg tcgggaattc accttccagc gatttcttaa
     1801 aatcgcgcaa gcgcgttatg atcttgccgg actcttcgag tttctcgaaa ctgaagttta
     1861 atttctgccg at
//
LOCUS       DAOSMP010000170         1845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_63360_length_1845_cov_20.074302, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000170 DAOSMP010000000
VERSION     DAOSMP010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63360_length_1845_cov_20.074302"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PKX09_10715"
     CDS             complement(<1..299)
                     /locus_tag="PKX09_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HOU18332.1"
                     /translation="MDPKTIEWSKLEFKYSYTPFRFQARWRDGHWDDGQLVTDKKISI
                     EEGATCLHYGQEVFEGMKAQRTRDGRVMLFRPRDNAARLRRSAARLLMAEVPEDL"
     gene            696..1772
                     /locus_tag="PKX09_10720"
     CDS             696..1772
                     /locus_tag="PKX09_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOU18333.1"
                     /translation="MYKKLFIPGPVDVRPEVLQAMATPMIGHRSKDASALQQRISEKL
                     QKLFFTQNPILLSTSSGTGLMEGSIRSCTAQRAMVFSVGAFGDRWAALAKANNVPCDL
                     VKAEPGYPTLPEIVDQYLSTGKYDVFTITHNETSTGLMNPVAEIAEVKRKYPDVYWLV
                     DAVSSMGGIKIPVDELGIDVCITSTQKCIGLPPGMAICSVSQRALEHARTVPFRGAYL
                     DLIELYDYLIKKDFQYPSTPSLSHMFALDLQLDYILNVEGLEKRYARHREMAEITRAW
                     AKENFAVFPQPGYESVTLTTITNTRGISISDLNKRLGEAGYQISNGYGELKEKTFRIA
                     HMAERTVDELKTLLNLIDTITAKM"
BASE COUNT          492 a          430 c          391 g          532 t
ORIGIN      
        1 aataaatctt ccggcacctc agccatcagt aggcgggcgg ctgaccgccg gagacgcgcg
       61 gcgttatccc gcgggcgaaa aagcataacc cgtccatcac gggtccgctg agccttcata
      121 ccttcaaaaa cttcctgccc atagtgcaaa caggtagcac cttcctcaat gctgattttt
      181 ttatcggtta ctaattggcc atcatcccaa tgaccatctc tccaacgcgc ctgaaaacga
      241 aagggcgtgt aggaatattt gaactcaagt ttactccatt cgattgtttt cgggtccatc
      301 attttcctct tttttaatcc attttatcgg caaacctgtt cggcttgctt tagttaattt
      361 aaccttcaaa aaacacttat ccttaatttt tttatcaaat gtaatgccac cagtcggtat
      421 gaatttgtta atctaatggg tagaggctga tttcatttga tttgggaaat taatagcatc
      481 tcctattaca gccaatatat tttcctaaaa ctggcaacaa ccagccggcg atttattagt
      541 tttaggaatg aatatttacg gagattttcc gatttctacg tatattcacc agagattttt
      601 atgaagtatt aaaatttggt tatgaatttg cgaacctact agctcaatgc aaagcagttt
      661 taaagttttg aactaaatca ccgtaggagg tcgtaatgta taaaaaatta tttattccag
      721 gcccagttga tgtccggcct gaagttttac aagctatggc gacgccgatg atcggacatc
      781 gttccaaaga cgcttccgct ctgcaacagc gtatctcgga aaaattacag aagttgttct
      841 tcactcagaa tcctatttta ctttcgacct cgtctggcac cgggctgatg gaaggctcaa
      901 ttcgttcctg caccgcccaa cgggcgatgg tcttttccgt cggtgctttc ggtgatcgct
      961 gggcggctct ggcaaaagcc aataacgttc cctgcgatct ggttaaagcg gaaccggggt
     1021 atcccacttt gccggaaata gttgatcaat atctctcgac cggtaaatac gatgttttca
     1081 cgattactca caacgaaacc tcgaccggtt tgatgaatcc ggtagctgaa atcgccgaag
     1141 tcaaacgcaa atatcccgat gtttactggt tagtggatgc ggtttcttct atgggtggca
     1201 ttaaaattcc ggtagatgaa ctcggtattg acgtttgcat tacctcgacg cagaaatgta
     1261 ttggtctgcc gcccgggatg gcaatctgtt cggttagtca gcgagcgttg gaacacgcgc
     1321 gtaccgtacc gtttcgcggc gcctatctcg atcttatcga gctctatgat tatttgatca
     1381 aaaaagattt tcaatatcca tccacacctt ctctgtcgca catgttcgct ctggatttac
     1441 agttggacta tattttgaat gtcgagggtc tggaaaagcg ttatgcccgt catcgagaaa
     1501 tggctgaaat tacgcgcgcc tgggctaagg aaaactttgc cgtctttccg caacccggct
     1561 acgaatcagt taccttgacc accattacca atacccgtgg tatttcaata tccgatttga
     1621 acaagcggct tggtgaagcc ggttaccaga tttccaatgg ctacggcgaa ctgaaagaaa
     1681 agaccttccg catcgcccat atggctgaac gcaccgttga tgagttaaag actttattga
     1741 acctcattga cacgataacc gctaagatgt aaaattcttg gccagaaaga aaagtatgaa
     1801 aatattaatt gccgacggtc tggatcaaaa agcccaaaat attct
//
LOCUS       DAOSMP010000171         1843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_63457_length_1843_cov_17.845078, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000171 DAOSMP010000000
VERSION     DAOSMP010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63457_length_1843_cov_17.845078"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="PKX09_10725"
     CDS             <1..993
                     /locus_tag="PKX09_10725"
                     /inference="COORDINATES: protein motif:HMM:NF028498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HOU18334.1"
                     /translation="AYLKSNNNVVGGTLKKVAGKFVYEGFADWWYESLMAYANAGATI
                     DYISIQNELNYEAPWESCVFAPSESDDTTLAAHNVAYETVWQKLNAKMGKTMPKMLTP
                     ETSGLGDSEVYIKNLIDLSHVYGYAHHLYDCSGCGADPDRFIPRMISYREMVKKYGAK
                     PIFQTEFEDDPGAWPDALNTALLLHNSLTVEQVSAYLYWDLFWAPGSGLISLNDFDSY
                     TIKPTYYAFKQYSAFIDANWQRVEVTNDNTGLRISAYVSPDNKKMTIVILNITQDMDI
                     NLKLAIKNFASAKGAIYRTSETENCVYIGEFNKRKPLVLPANSITTLSLSTEEK"
     gene            1182..>1843
                     /locus_tag="PKX09_10730"
     CDS             1182..>1843
                     /locus_tag="PKX09_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOU18335.1"
                     /translation="MGFLSFVSAEPTKWVGTWSTAPQLVETNNNPPSPGLSNNTLRQI
                     VRVSIGGDTLRLRFSNEFSTKPVTMNSVHIAISTGGGTIDTATDKSLFFNGSPNVTME
                     AGKAVTSDPFQFTLPPRADIAITIYFGATSPDVTGHPGSRTTSYLLTGNAVTRADCAG
                     AVTTDHWYVINTIDVKAPDSAAAVVILGNSITDGRGSGTNKQNRWPDELARRFQENPA
                     TR"
BASE COUNT          510 a          444 c          406 g          483 t
ORIGIN      
        1 gcttatttga agagtaataa caatgtggta ggtggaacgc tcaaaaaggt cgccggaaaa
       61 ttcgtttacg aaggatttgc cgactggtgg tatgaaagtc tgatggctta tgccaatgct
      121 ggggcgacca tagattatat cagcattcag aatgaattga attacgaagc gccctgggaa
      181 tcgtgcgtat ttgctcccag cgaatctgac gataccacgc ttgccgctca taatgtagcc
      241 tacgaaaccg tctggcaaaa gttgaacgct aaaatgggca aaaccatgcc gaaaatgctt
      301 acgcctgaaa cttctggcct gggagattct gaagtatata tcaaaaattt aatagatttg
      361 tcgcatgtct atggctatgc tcatcatctt tacgattgta gtggatgtgg ggcagatccc
      421 gatcgcttca ttccgcggat gattagttat cgggaaatgg tcaagaaata tggcgccaaa
      481 cccatttttc agactgaatt cgaggacgac cccggtgcct ggccggatgc cctcaatacc
      541 gcgctattgt tgcataattc cctgacagta gagcaagttt ctgcctatct gtactgggat
      601 ttattctggg cgcccggttc cggtctgatt agtctgaacg atttcgattc ctacacaatc
      661 aaacccactt attacgcctt taaacagtat tcagctttca ttgacgctaa ttggcagaga
      721 gtcgaagtca ccaatgataa taccggcctt agaatttccg cgtatgtcag tccggataat
      781 aaaaaaatga cgatcgtgat tctcaatata actcaggata tggacattaa tcttaaactt
      841 gcgatcaaaa attttgccag tgccaaaggc gcaatctatc gcaccagtga aaccgagaac
      901 tgcgtctata tcggtgaatt caataaaaga aagcccttag tattgccagc caactctatc
      961 acgaccctct cgctttctac cgaggaaaag taactgagat tacctgccaa agagcgggta
     1021 tctctaaaat gtcgtttcac gggtgaaaaa tttgattttc ccaatatgaa aggagaaaat
     1081 gctatatgaa atcggtaaga cacactatcg ctaatggcgg acttaatcgc cagcatcttc
     1141 aatatcaaac ttctgccctt gtattggtaa ttcttttaat tatgggattt ctttcctttg
     1201 tttctgctga accgaccaaa tgggtgggca cctggagcac tgccccacaa ttggtggaaa
     1261 caaacaataa tccgccaagc cccggcttga gtaataatac cctgcgccaa attgtccgcg
     1321 tttccatcgg tggtgacacc ctgcggctac gcttttccaa tgaattcagc accaaaccgg
     1381 tcacgatgaa ttcagttcat atcgctattt caactggtgg tggtacgatt gacaccgcca
     1441 cggacaaatc gctatttttc aacggctctc cgaatgttac catggaagca ggtaaagccg
     1501 ttacctcaga tcccttccaa ttcaccttgc cacccagggc ggacattgcc ataactattt
     1561 attttggcgc aacttctccg gacgtaaccg gtcaccccgg ctcgagaact acatcatacc
     1621 tactcaccgg gaatgcagtt accagagcag attgcgccgg tgcagtcaca actgatcact
     1681 ggtatgttat taataccatt gatgtaaaag cgccggactc agccgcggcg gtggttattt
     1741 tgggtaattc catcacagat gggcgcggct ccggcacaaa caaacaaaac cgctggccag
     1801 atgaactggc gcgtcgcttt caggaaaatc cggctacccg ttt
//
LOCUS       DAOSMP010000172         1826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_64287_length_1826_cov_17.708639, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000172 DAOSMP010000000
VERSION     DAOSMP010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64287_length_1826_cov_17.708639"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /gene="hypD"
                     /locus_tag="PKX09_10735"
     CDS             complement(<1..1031)
                     /gene="hypD"
                     /locus_tag="PKX09_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOU18336.1"
                     /translation="MIKYIDEYRNPELIHKAVERIRAITPNKIVNLMEVCGGHTITIF
                     KFGLKKLLPENIHLISGPGCPVCVTDSEFIDRAIEIAKFSNVIITTFGDMVRVPGSYG
                     SLQSARAGGADVRTCYSPLEAVEIAERNPAKEVVFLGIGFETTAPNIASAILNANSRH
                     ISNFSVMSGHKTMPKAMQSLLDSGEIRLDGFICPGNVTAITGPEIYQFIADEYKIPCV
                     VAGFEPLDMLEAIFMLVKQIVECRAEVENQYRRTVKPGGNRQAQRIMAEVFEPGDTIW
                     RGMGNIPRSGLQIRSKYRQFDADYKFQVKLPPTKSVSGCICGDIMRGVRIPPECRLFR
                     KICTPDNPQG"
     gene            complement(1028..1264)
                     /locus_tag="PKX09_10740"
     CDS             complement(1028..1264)
                     /locus_tag="PKX09_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOU18337.1"
                     /translation="MCLAVPMRIIEIDGTRAKAEVGGVLYQANLDFLSDVKVGDYIIV
                     HAGFAIEKLDEIVALENLAAWQEVATDVKTKYRK"
     gene            complement(1264..>1826)
                     /locus_tag="PKX09_10745"
     CDS             complement(1264..>1826)
                     /locus_tag="PKX09_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOU18338.1"
                     /translation="PPQQIGIVNDMLKSGFNAPLTTSCGRLFDGVAALAGLRNRVNYE
                     GQAAVEFEQCIASENYRSAYDFEIETGTDSFVIDWRVMIEQVIEDVKLQTGIELIAVK
                     FHNGLANILLQAALKSREMTDFNTVALSGGVFMNIYLLTRLSQLLNEHNFKVCTHHLV
                     PCNDGGIALGQAVIANAIIQNQLEKS"
BASE COUNT          495 a          445 c          380 g          506 t
ORIGIN      
        1 ccttgtggat tatccggagt acagattttc ctaaataacc gacattccgg tggaattctg
       61 acgccacgca taatatctcc acaaatacaa cctgaaacac ttttagtggg aggcaatttt
      121 acctgaaatt tataatcggc atcaaattga cggtatttgg atcgtatttg aagaccgctc
      181 ctaggaatat tgcccatccc gcgccagatt gtatcgccgg gttcaaaaac ttctgccatt
      241 attctctggg cttggcggtt accacccggt ttgactgtgc ggcgatattg attctcgact
      301 tccgcccgac attctacaat ttgtttaacc agcataaaaa tcgcctcaag catatcgagc
      361 ggctcaaatc cagctacaac acatgggatt ttatattcgt cagctatgaa ttggtaaatt
      421 tcggggccag ttatagcagt aacatttcct gggcaaataa aaccatccaa ccttatttcg
      481 ccggaatcca gcagagattg cattgctttc ggcatagttt tatggcctga catcaccgaa
      541 aaatttgaga tatgccgact attagcattt aaaatcgccg aggcaatatt cggtgcagtc
      601 gtctcaaaac cgattccaag gaaaacgacc tctttcgccg gattccgctc agcgatttca
      661 accgcctcga gcggcgaata gcaggtacgc acatcggcac ccccagctcg ggctgactgc
      721 aaactaccgt aacttccagg aactcgcacc atatcaccga aagttgtaat tatgacgttc
      781 gagaatttag caatctcgat cgcccgatca ataaattcac tatcggtcac acagactgga
      841 cagcctggcc cgctaatcag atggatattc tccggtagca attttttcaa tccgaattta
      901 aaaatggtaa ttgtatgacc gccacagact tccattagat taacaatttt attcggtgta
      961 attgctcgaa tgcgttcaac agctttgtga attaattccg gattgcggta ttcgtcaatg
     1021 tatttaatca ttttcggtat ttggtcttga cgtcggtagc aacttcctgc caggcggcga
     1081 gattctcgag cgccacgatt tcatccaatt tctcaatggc aaaaccggcg tgtacgatga
     1141 tataatcacc gactttaacg tcactcaaaa agtcgaggtt agcttggtat aggacgcctc
     1201 cgacttcggc tttggcgcgg gttccgtcaa tttcaataat tctcatcggc acagccagac
     1261 acattatgac ttctccaatt gattttgaat gattgcgttg gcaataaccg cttgcccgag
     1321 agcaattcca ccgtcgttgc aaggtacaag atgatgggtg cagactttga aattgtgctc
     1381 gttaagcaat tgcgatagac gcgtcagcaa gtaaatattc ataaaaacgc ctccgcttag
     1441 cgccaccgta ttaaagtcgg tcatttctcg ggatttcaaa gcggcttgca gaagaatatt
     1501 tgccaatccg ttatgaaatt tgacagcgat tagctcgatt ccagtttgaa gtttgacatc
     1561 ctcaatgact tgttcaatca taacgcgcca atcaatgaca aagctatcag ttccggtttc
     1621 gatttcaaag tcataagccg agcgataatt ttcagatgcg atgcattgtt caaattccac
     1681 cgccgcttgg ccttcataat ttacccgatt acgtaaacct gccagtgccg ctaccccatc
     1741 gaaaaggcga ccgcaggaag tcgtcagtgg cgcattgaaa ccggatttca gcatgtcatt
     1801 cacgatcccg atttgttgtg gcggaa
//
LOCUS       DAOSMP010000173         1818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_64670_length_1818_cov_18.319342, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000173 DAOSMP010000000
VERSION     DAOSMP010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64670_length_1818_cov_18.319342"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PKX09_10750"
     CDS             <1..561
                     /locus_tag="PKX09_10750"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOU18339.1"
                     /translation="GQYKEYCRQTGKSMPEQEAGTSDSYPIAFVTWYEANEFCQWVGG
                     YLPTEAQWEYAARSRGLNLKYPNGNTIDHSFANYSGTGQTDKWKRVAPVAKFPPNSLG
                     LYDMAGNLYEWCQDYYRSDYYKNSSQINPTGPASGMFKVIRGGSWYHGKEAMRTTSRY
                     RYMPVARLSFLGFRVAWDPDAVRMAE"
     gene            608..>1818
                     /locus_tag="PKX09_10755"
     CDS             608..>1818
                     /locus_tag="PKX09_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18340.1"
                     /translation="MRRDYLKFAVMPAVMGAVAMIAQVAYLRLTAQSFYGNELTMCIA
                     LGHWLIWTGLGSLVGARLVRRNLSEKWLTAIVLSYGMVLIVFAGLLFLNRRIAGIPAG
                     TVVGLGTIFAWTFILFSLPAWLNGLFFPLIVNWVKVKADLSPVHTVYAGEVIGSALGS
                     LLFAGLILSGVSTIDCLLVIVWVMMILAIGLFIYSERMRIFLTIGTSLIVILASVGFR
                     GPITNWKWAPFRALLSRESPHLALTTVRYHDAISVYGDSQPLWTFGDREKAEEWTHFA
                     LLNHPDPRSVLVIGIGSIEICREISRHPSVEWITILQPDRILHQMVTQFGSIDALDTN
                     VQVIFADPALYLGKTDERFDVVLMNIPLPVNAQWNRFYTREFFEIVHSRLSPGGLLML
                     NLPGDEEFLPPA"
BASE COUNT          420 a          368 c          474 g          556 t
ORIGIN      
        1 ggtcaataca aagagtattg tcgtcaaaca ggtaaatcta tgccggaaca ggaagcggga
       61 actagtgatt cttacccgat tgcatttgtt acctggtatg aagccaacga attttgtcag
      121 tgggttgggg gttatttgcc tactgaggcg caatgggagt atgccgctcg ttcgaggggt
      181 ttgaacttga aatatccgaa tggcaatact attgatcatt cctttgctaa ttattccgga
      241 accggtcaaa ctgacaaatg gaaaagagtt gcgccggtgg caaaatttcc accgaatagc
      301 ctcggtcttt atgatatggc tggcaatctt tatgaatggt gtcaagatta ttaccgcagt
      361 gattactaca aaaattccag ccaaataaat cccacggggc cagcttccgg tatgttcaaa
      421 gttatccggg gaggaagttg gtatcatggc aaagaagcaa tgcgaacgac cagtcgttat
      481 cgctatatgc cggttgctcg gctaagtttt ctcggattca gagttgcctg ggacccggat
      541 gcggtccgaa tggcggaata gtttttatta ataaaattta ccttaagatt ttcgcatggg
      601 catttttatg aggcgagatt atttaaaatt tgctgtaatg cccgctgtga tgggcgcggt
      661 tgctatgatt gcgcaggttg catatttgcg cctgacggct cagtcatttt acggcaatga
      721 actgacgatg tgcattgcac tcggtcactg gttaatctgg actgggctgg gtagtttggt
      781 aggtgcgcga ttagtgcggc gcaatttatc ggaaaaatgg ttgaccgcaa tcgttctaag
      841 ctacggaatg gttctgattg tttttgctgg actgttattt ttaaatcgcc ggattgccgg
      901 gattcccgcc ggcacagtag tcggcttggg aacgattttc gcctggactt ttatcctttt
      961 cagtctgcca gcctggttga atggattgtt ttttccgcta atcgtgaatt gggtcaaagt
     1021 taaagcggat ctttctccgg ttcatacagt ctatgctggt gaagtaatcg gctcggcttt
     1081 ggggagtttg ctttttgccg gtttgattct atccggggtg agtactatag attgcctgtt
     1141 ggtaattgtg tgggtaatga tgattttagc gatcggactc tttatttact ccgaacgaat
     1201 gcgaatcttt ttaaccatcg ggacttcgct gatagttatt ttggcttcag ttggtttccg
     1261 agggccaatt actaactgga aatgggcgcc gtttagggct ttgctcagcc gcgaatcgcc
     1321 gcatcttgca ttgactaccg tacgttacca tgacgccatc agcgtctatg gcgatagtca
     1381 gccgctctgg actttcggtg accgcgagaa agccgaggag tggacgcatt ttgctttgct
     1441 gaatcatcct gatccgcgat cggttttagt cattggaatc ggaagcattg aaatctgtcg
     1501 cgaaattagt cggcatccgt cggtcgagtg gattacaatc cttcagcctg accggattct
     1561 gcatcaaatg gttactcaat tcggatcaat tgatgcgtta gacaccaatg ttcaggtcat
     1621 tttcgctgat ccagctttat atttagggaa aactgacgaa cgattcgatg tggttttaat
     1681 gaatattcct ctgccagtca atgcccagtg gaatcgcttt tatactcggg aatttttcga
     1741 aatcgtgcat agccgattaa gtccgggcgg gcttttgatg ctaaatttac ccggcgatga
     1801 ggaatttttg ccaccggc
//
LOCUS       DAOSMP010000174         1815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_64831_length_1815_cov_14.126136, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000174 DAOSMP010000000
VERSION     DAOSMP010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64831_length_1815_cov_14.126136"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PKX09_10760"
     CDS             complement(<1..560)
                     /locus_tag="PKX09_10760"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOU18341.1"
                     /translation="MANQRKRKVENGLVEGRLSRSIWKLAAPMMIGGALLDLFSIVDL
                     FFVGRLGHIEVAALSIAGTVMAIMMMLVQGIGVGTTALISHFTGEKNYEKSDEVLGQT
                     LILGLIGSAIMLVVSFFLIEPLLKLFGATGEVLTYAADYLRINFNYSIIIFLFSGVNS
                     ALQGSGDVRTPLNALIIGNVLNIILDP"
     gene            820..1575
                     /locus_tag="PKX09_10765"
     CDS             820..1575
                     /locus_tag="PKX09_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU18342.1"
                     /translation="MTKTILITGTTSGFGKSIAERFAAEGWNLILIGRRSDRLMAISA
                     KLGEKVTVLPITLDIRQRQAVMETLTNLPEKFKAVDVLVNNAGLAAGLEPAQSADLDD
                     WDAMVDTNIKGLMYVTRAILPGMVTRNAGHIINIGSVAGSYPYEGGNVYGATKAFVAQ
                     FSRNLRTDVSGTAIRVTNIKPGNAETEFSLIRFKGNVAKAQAVYQGYQPLTAEDIAEM
                     VFWVSHLPAHVNVNELEVMPVAQSWAGYKIHRQ"
     gene            1634..>1815
                     /locus_tag="PKX09_10770"
     CDS             1634..>1815
                     /locus_tag="PKX09_10770"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the isomerization of isopentenyl
                     pyrophosphate to dimethylallyl diphosphate; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOU18343.1"
                     /translation="MIHKITERKKDHLRIICEENVQYEKVTTGFEQFTFIHNALPEID
                     LREVDCTCEFLGYQLQ"
BASE COUNT          557 a          377 c          340 g          541 t
ORIGIN      
        1 ggatcaagaa taatattaag aacatttcct attatcaggg catttaaagg cgttctgaca
       61 tctccggaac cctgtaatgc actattcaca ccgctaaaaa gaaaaataat tatagagtag
      121 ttaaaattta ttcgcagata atcagcggca taggttaata cttcacccgt ggcaccaaaa
      181 agtttcaaaa ggggttcaat tagaaaaaac gagacaacta gcattatagc cgaaccgatt
      241 aatcctaaaa taagcgtctg ccccagaact tcatccgact tctcataatt cttttcaccg
      301 gtaaaatgcg aaattaaagc tgttgtccct acacctatac cctgaactaa catcatcatg
      361 attgccataa ctgtcccggc aatagagaga gcagctactt ctatatgtcc caacctgcca
      421 acaaaaaata aatctactat cgagaaaagg tcaagcaaag ccccgccaat catcatcgga
      481 gccgctagtt tccagatcga acgcgaaagt cggccttcta ctaaaccatt ttcaaccttc
      541 cgcttgcgtt gatttgccat attattcctt agtgtatcgt aagagaatgg agtaagtcaa
      601 atatttatac atttttgtag tgtttctgac ttatattttt attttttata attctaaaca
      661 tcttcaaact ttggatttta attttgccag ctgagatttc caatagtttt tatgtacatt
      721 tttataattt aaatttaatt tgtattcgct tctcgatcgc attttccagt tgcattttta
      781 aattgttact ctgaaatttt cgcctatgaa aggagaaata tgactaaaac aattttaatc
      841 acaggaacca cttccggatt tggaaaaagt atcgccgagc gtttcgccgc agaaggatgg
      901 aatttgattc tgataggtcg tcgctccgat cgccttatgg ctatttcagc caaactcggc
      961 gaaaaagtaa ctgtattacc aattactctc gatattcgtc agcgtcaggc agttatggaa
     1021 actctcacca atttaccgga aaaatttaag gcagtggatg ttctggttaa caatgccggt
     1081 ttagccgccg gtcttgaacc tgctcaatcg gccgatcttg atgactggga tgcgatggta
     1141 gataccaata tcaaaggctt aatgtatgtg actcgggcga ttttgcccgg tatggttacg
     1201 aggaatgccg ggcatattat taatattggc tcggtagccg gctcctatcc ttatgaaggc
     1261 ggtaatgtct atggcgccac taaagctttc gtagctcagt ttagtcgtaa tctccgaaca
     1321 gatgtcagcg gaacggcgat tcgagttact aatatcaagc ccgggaatgc cgaaaccgaa
     1381 ttttcactga tccggttcaa aggtaacgtt gccaaagctc aagcagtcta tcaaggctat
     1441 cagcctttga ccgctgaaga tattgctgaa atggttttct gggtctcaca ccttccagca
     1501 catgtcaatg tcaacgaatt agaagttatg ccagttgctc aatcctgggc tggttacaaa
     1561 attcaccggc aataatattt atactgataa acataatttt gaattataat gacgattaaa
     1621 tagatttgcc gcaatgattc ataaaataac cgagcgtaaa aaagatcatc tccggattat
     1681 ttgcgaagag aatgttcagt atgaaaaagt aacaaccggt tttgaacaat ttacctttat
     1741 tcataacgct ttaccagaaa tagacctccg tgaggttgat tgtacctgcg aatttctcgg
     1801 atatcaactc caaat
//
LOCUS       DAOSMP010000175         1811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_65007_length_1811_cov_19.115034, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000175 DAOSMP010000000
VERSION     DAOSMP010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65007_length_1811_cov_19.115034"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..597
                     /locus_tag="PKX09_10775"
     CDS             43..597
                     /locus_tag="PKX09_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOU18344.1"
                     /translation="MLETFERIQLVSGDITREKCDAIVNAANRSLLGGGGVDGAIHRA
                     AGPRLLEECRQIREQLLPDGLPTGEAILTAGYDLPARFVIYTVGPVWNGGKHGEDELL
                     AACYRNVLRVAAEKKFDTIAIPAISTGAYRFPLERAAEIALKVIVQHLANNEFPRTVR
                     CVLIGDNYRIYERLLNQMHSSTVR"
     gene            694..>1811
                     /gene="pgi"
                     /locus_tag="PKX09_10780"
     CDS             694..>1811
                     /gene="pgi"
                     /locus_tag="PKX09_10780"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOU18345.1"
                     /translation="MLPSVNPTSTSAWQKLLAHYQKIKKTSIRNLFKDDPERFQRFSI
                     NLVLDETSRDEILLDYSKNLITPETFQLLLKLARECGVAEAIELMFTGAAINVTENRA
                     VLHTALRNLDDNPIYVDGQNVMPPIKAVQEQMREFSAKVINGEWRGYDGQPIKDVVNI
                     GIGGSDLGPAMVTEALKPYHIPHLKLHFVSNVDGTDICETLKCLNPATTLFIIASKTF
                     TTQETITNAQTARNWFLTTVGEPDFIKSHFVAVSTNRAKVIEFGIDPENMFVFWDWVG
                     GRYSLWSAIGLSITLAVGFDNFRELLAGAYAMDNHFRQTPFEKNIPVILALLGIWYNN
                     FFGAETLAILPYDQYLRRLPAYLQQADMESNGKRVDRNG"
BASE COUNT          487 a          381 c          444 g          499 t
ORIGIN      
        1 aagaattttt cagatgaaaa tcaattaaga tcagtgacga aaatattaga gacattcgaa
       61 cgaatccaac ttgtctccgg tgatattact cgggaaaaat gcgacgctat cgtcaatgcc
      121 gccaatcgtt cgcttttggg tggcggggga gtggacggtg ctattcatcg tgcggctggt
      181 ccgcgccttt tggaagagtg tcggcaaatt agggagcaat tattaccgga cggcttacca
      241 actggtgaag cgattttgac ggcaggctac gacttgccgg ctcgttttgt gatttacact
      301 gtcggcccgg tttggaacgg tggcaaacac ggtgaggatg aactcttagc cgcctgttat
      361 cgaaacgttt tgcgtgttgc ggccgagaaa aagttcgata ccatcgccat tccagctatt
      421 agtaccggtg cctaccgatt tccattagaa agggcggctg aaatcgcgct aaaggtcatc
      481 gtacagcacc tagcgaataa cgaatttccg cgaaccgtgc gctgtgttct catcggcgat
      541 aactaccgga tttacgaacg attgctgaat caaatgcatt cgtcaactgt aagataattc
      601 cacgagtaca attgttcaat ttttggggtg aattattgga ttcgggaatt tttttttgta
      661 atctttggcg tttaatgaca ggagaatata aaaatgttac cttcagtcaa tccgacttcc
      721 acttccgctt ggcaaaagct gttagcccat tatcagaaaa taaaaaaaac atcaataaga
      781 aatttattca aagatgaccc ggagcggttt cagcgttttt cgatcaattt agttctcgat
      841 gaaacgagtc gagatgaaat tctgctcgat tatagtaaaa atctaattac acctgaaact
      901 tttcaacttt tactaaaact ggcgcgagaa tgcggcgtcg ccgaggcgat cgaattgatg
      961 ttcaccggcg cggcgattaa tgtcaccgaa aatcgcgcgg ttctccacac ggcattgcga
     1021 aatctcgatg ataatccgat ttacgtcgat ggtcagaatg taatgccgcc aataaaggct
     1081 gtccaggaac aaatgcgtga attcagtgcc aaagttatca atggcgaatg gcgtggttac
     1141 gatggtcagc cgatcaaaga cgtggttaat attggaattg gcggcagtga tctgggaccg
     1201 gcgatggtga ccgaagccct aaaaccttat cacattccgc atctaaaatt gcattttgtt
     1261 tccaatgtag atggcaccga catctgtgaa actttgaaat gtctaaaccc ggcaactacg
     1321 ctttttatca ttgcctccaa gacttttacg acgcaggaaa cgataactaa tgcccagacg
     1381 gcgcgcaatt ggtttctaac cacggtcggt gagcctgatt tcatcaaatc gcactttgta
     1441 gcggtttcga ctaatcgggc aaaagtaatc gaatttggaa ttgacccgga aaatatgttc
     1501 gtgttctggg attgggtcgg cggtcgctat tcgctctggt cagcaatcgg tttatcaatt
     1561 acgctggcgg tcgggtttga taattttagg gaattgctgg ctggcgccta tgctatggat
     1621 aaccattttc gccagacgcc ttttgaaaaa aatattccgg tgattctggc attgctggga
     1681 atttggtata acaatttttt cggggcagaa acgctggcta ttttgcctta tgatcaatat
     1741 ttaaggcgcc tgccggctta tctccaacag gcggatatgg agagtaacgg caagcgagtt
     1801 gatcgcaacg g
//
LOCUS       DAOSMP010000176         1810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_65055_length_1810_cov_8.392023, whole genome shotgun sequence.
ACCESSION   DAOSMP010000176 DAOSMP010000000
VERSION     DAOSMP010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65055_length_1810_cov_8.392023"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1810
                     /locus_tag="PKX09_10785"
     CDS             <1..>1810
                     /locus_tag="PKX09_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18346.1"
                     /translation="DAIPIATWEEGATCLITGIAGTSAPTGLNQEFYHFTWNCPSQSG
                     NLNFNANLTTIKGDFNVLNTNGKQLRLGSKDLTVNISGDVNIGPNALLTSSGSAGAYT
                     ITVNIGGDINISEGMLYLENAGELVANWNLAGDFNMFNGTLGRQGSGTGNLNFLSGSH
                     TFRRSGGTITSNNGGPIFNVKTEAILDLGTSRIEQGPFNLDSAATLICGNPGGLDSSL
                     TTPRVITLSPYANYIFNSTSEQVTGQLLPAVVNNLTFENSSGDTLSHNVTVNGLLTVN
                     SGYLEISGDTITLGPSAMLVETGGPVKGDGIITTTRTLNNISAENVAGLGAVITTTKD
                     MDSTIVTRGHSINSLNGAPSIRRYYDILPKNNTGLNATLVFKYDESELGDLSETDQLA
                     LFSRSEEASFWSERGGILDTANNQIALAGIDKFSKWTIGMHVNSAPLPFTMLKPTEGY
                     EVTNSDIVQDSLIEVKWSRAVDPDNEALTYKLFILNTDKDSVVISVETPDTSILIDAP
                     TYKDNGNYQAYVLAYDTWEAFSSSDTVDFTINLTPEGIKGDLVPKVLALHQNYPNPFN
                     PITTIKYDLPEEVHVKLVIYDIMGREIRTLVNERQQA"
BASE COUNT          521 a          362 c          405 g          522 t
ORIGIN      
        1 aagatgccat tcctattgca acctgggaag aaggggcgac ctgtttgata accggtatag
       61 ccggtacctc agcgcctaca ggacttaacc aggagttcta tcatttcacc tggaattgcc
      121 catctcagag cggaaatctc aattttaatg ctaatctgac caccattaaa ggcgatttca
      181 atgttctaaa taccaacggt aaacagttac gattgggtag taaagatctg actgttaata
      241 tcagcggtga tgtgaatatc ggaccaaatg cattgctaac cagctctggt tcagctggtg
      301 catacacgat tacggtaaac attggtggtg atattaatat ttccgaagga atgttatact
      361 tagaaaatgc cggggaatta gtcgccaact ggaatctggc gggtgatttc aatatgttta
      421 atggaacatt aggtcggcaa ggctcaggaa ccggtaattt aaactttcta agcggttcac
      481 acactttcag gcgctcaggt ggaaccatta ctagtaataa cggtggccct atttttaatg
      541 tcaaaacaga ggctatcctg gatttaggca ctagccgtat cgaacaggga ccttttaatt
      601 tagattctgc ggcgacttta atttgtggta atcctggtgg acttgacagc agtttaacga
      661 cacccagagt gattactctt tctccgtatg ctaattatat tttcaacagt acaagcgaac
      721 aggttaccgg ccagctatta ccggcggttg tcaataacct cacctttgag aattccagtg
      781 gtgatacctt atcgcataat gtcaccgtca acggtttact gactgtgaat agtggttatt
      841 tagaaattag tggcgatact attacgcttg gaccatccgc tatgcttgta gaaaccggcg
      901 gtccagttaa aggtgatggt attataacta cgactcggac tttgaataat attagtgctg
      961 aaaatgtcgc tggcttgggt gccgtaataa ccactacgaa agatatggat tcaactatcg
     1021 taacccgtgg ccattcaatc aattctctta atggcgcacc cagtattagg cgctattatg
     1081 atattctgcc gaagaataat accggactca atgcgacact ggtttttaaa tatgacgaat
     1141 cagaattagg tgatttgtct gagactgatc aactggcgct ttttagtcgt tctgaggagg
     1201 catctttctg gagtgaaaga ggtggtatat tggatactgc caataatcaa atagctttag
     1261 caggcattga taaattctca aaatggacta ttggtatgca cgtaaattca gcgcctttac
     1321 cctttactat gctaaagcct actgaaggct acgaagttac taatagcgat attgttcagg
     1381 attcactgat tgaggtaaaa tggtcgagag ccgttgaccc ggataatgag gcactgactt
     1441 ataaattatt tatcttgaat acggataaag actcggtggt catctcggtt gaaacaccgg
     1501 atacatccat tctgattgat gcgccgactt acaaggataa tggtaattat caggcgtatg
     1561 ttctggctta tgatacctgg gaggctttca gcagttccga tacggttgat tttaccatta
     1621 atcttactcc ggaaggaatc aaaggtgatt tggttccaaa ggttcttgcc ctgcaccaga
     1681 actacccgaa tccgtttaac ccgattacca cgataaaata cgacctacct gaagaagtac
     1741 acgtcaaatt ggtcatttat gatattatgg gacgggaaat tcgcactctg gtcaatgaga
     1801 gacagcaagc
//
LOCUS       DAOSMP010000177         1782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_66497_length_1782_cov_16.363057, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000177 DAOSMP010000000
VERSION     DAOSMP010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66497_length_1782_cov_16.363057"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..263
                     /locus_tag="PKX09_10790"
     CDS             3..263
                     /locus_tag="PKX09_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18347.1"
                     /translation="MTKSFMIIVVNLRKKEAVNVQKILTEWGCLIRTRLGLHEGVLDQ
                     CSDVGSIILELVGERDKHIELARKLNLLSGVKAQYIEIPVEM"
     gene            289..1266
                     /gene="pfkA"
                     /locus_tag="PKX09_10795"
     CDS             289..1266
                     /gene="pfkA"
                     /locus_tag="PKX09_10795"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOU18348.1"
                     /translation="MGVLKRIAILTSGGDAPGMNACVRAVVRTAISDGIDVFGVYGGF
                     EGLINGDVIPLNRRSVSNMIQKGGTFLKTSRSEAFRHKSGRKRAAEHLAEWKIDGLVV
                     VGGDGSFRGAHDLYEESGIQVVGIPATIDNDIYGTDITIGYDTAVNTALDSIDKIRDT
                     AASHDRLFIVEVMGRSAGFIALEVGICGGAEEILVPETKVTLENLGNLIKTWAIAGKT
                     SSLIVVAEGDELGNAHEIAAKLYQQYHTICHVCVLGHIQRGGSPTATDRLLASRLGYH
                     AVKAIEDGKSDVMVGWINNSVTFIPLPESWEKKKPLDEELIKIYRILST"
BASE COUNT          531 a          319 c          428 g          504 t
ORIGIN      
        1 ctatgactaa aagttttatg attatcgtcg taaatctgcg taaaaaagaa gcagttaatg
       61 tccagaaaat tttgaccgaa tggggttgct tgatccgaac ccgacttggt ctgcacgaag
      121 gtgtattgga tcaatgttcg gatgtcggtt cgattattct ggagcttgtc ggcgaaagag
      181 ataaacacat cgaattagcc cgcaaactca acctgctatc tggtgtcaaa gcccagtata
      241 tcgagattcc cgttgagatg tgagttttaa atttagaaag gtaaatatat gggcgtttta
      301 aaaagaattg cgattctcac cagtggaggt gatgcgccgg ggatgaatgc ctgcgtaaga
      361 gccgtagtac gaacagccat ttctgatggt attgatgttt tcggagtata tggtggattc
      421 gaaggtttga ttaacggcga tgttattccg ttgaaccgac gttcggtaag caatatgatt
      481 caaaaaggcg gcactttcct caaaacttct cgtagtgagg cttttcggca taaatcgggt
      541 cgcaaacgcg ccgctgaaca cttggctgaa tggaaaattg acggtctggt agtagtcggt
      601 ggagatggca gtttccgagg tgcccacgat ctttatgaag aaagcggaat ccaggttgta
      661 ggtataccag ctacaattga caatgatatt tatggtactg atataactat cggctatgac
      721 acggccgtaa acacggctct cgattcaatt gataaaattc gggatacagc cgcatcgcac
      781 gaccgactct tcattgtcga agttatggga cggagcgccg gatttattgc cttagaagtc
      841 gggatttgtg gtggagcaga ggagattcta gtgccagaaa ccaaggtgac tctggagaat
      901 cttggtaatt tgataaaaac ctgggcgatc gccgggaaaa ccagcagtct aatcgtagta
      961 gcggaaggtg atgaactcgg taatgcccat gagattgccg ccaaattata ccagcagtat
     1021 cacacgattt gtcatgtttg tgtgctcgga catattcagc gtggcggtag ccctaccgca
     1081 accgaccgtt tattagccag tcggttaggc tatcatgcag ttaaggcaat tgaggatggc
     1141 aagtccgatg ttatggtcgg ctggataaat aactccgtga catttattcc tttaccggaa
     1201 agctgggaga agaaaaaacc actcgatgaa gagctaataa agatttatcg catcctatca
     1261 acttgattac acaaattcca agcttttaaa cagtatcctg cttaatgcta gcgctattta
     1321 ttgggaatag acgggctggc gaggttggtt attacctcac gggtattgtt tcaaaattac
     1381 gaatagaaat aatctattac catcgtccca gtcgagggat ttcaccgtca aaatgactga
     1441 aaaattgacc tgtatgccac aagtgtgata gttcgaacta atcactttag gatcggactg
     1501 aatttaaagc gttgtaaata tattggaata caatcggcag gcgttgataa aaatatggta
     1561 ggaattggat tcgaggaaaa gttaattgcg cttaatttga gaagtaaata ttgaatcaat
     1621 tccggaatga aacatccaaa atcgagatga aacatagcta atctaaccag tatctactta
     1681 ttttcgggct ttggaaaata atctcgtttt tttttagcaa tgttttaaat tagcctgaat
     1741 aattcataaa aataaagatg tttggttgtt taatacattg ta
//
LOCUS       DAOSMP010000178         1762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_67550_length_1762_cov_15.493263, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000178 DAOSMP010000000
VERSION     DAOSMP010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67550_length_1762_cov_15.493263"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="PKX09_10800"
     CDS             <1..1585
                     /locus_tag="PKX09_10800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU18349.1"
                     /translation="PVYTGNPYLAMNIYVTDAKIDGVTLGVGDEIGIFDGDICVGADV
                     VTGTIAPYLPLVAATDDPTTTEKDGFTPGHTISYRLWDKDQSLEINSVQPTYTQGEGF
                     FSSQGTAVVSLVGSYSISQTIGLDLGWNIVSFCVIPENPDMLNVFQNLVNNNSLIKIQ
                     NETGQAVEKLGAIGWVNNIGNLTATEGYYVKVASNQTLTIEGNGIPLPLGIPLTSGWN
                     IMSYPASSAQNALDVIQPLIDDGKLQKIQDEVGNAIENLPTIGWVNNIGNFEPGDGYY
                     IKVNSTTSVTVDQPTGSPLAKSQMATLAVSPTHYLPITAMNPYLPMNVYVMSATVEGE
                     PLKTGAEIAIYHQDRCITAAALPYPLNTENAYLPLIIGADDPLSEEIEGFNENDPISF
                     KIWDGNEELLVSVKVIKYGDNPGILKFNAHGTMVVELEAQRIPKEYSLHQNYPNPFNP
                     TTTLRYELPKPGKVEIRIFDLQGRLVNQLVSEYQEVGKYSVVWNGCDNHGIPVPSGIY
                     FYQISTGSYSKTRKAILMK"
BASE COUNT          517 a          391 c          380 g          474 t
ORIGIN      
        1 cccggtttac actggcaatc cctatctggc gatgaatatc tatgttacag atgctaaaat
       61 agacggggtt actttaggcg ttggtgatga aattggtatt ttcgacggtg atatttgtgt
      121 gggagccgat gtagtgaccg gcacaatagc cccatacctg ccgctggttg ccgctactga
      181 tgatccaact acaactgaaa aggatggttt tactccggga catacgatca gttaccgact
      241 ctgggacaaa gaccaaagcc ttgaaatcaa tagcgtccag ccgacttata ctcaaggtga
      301 agggtttttc tcgtctcagg gaacggcagt ggttagtctg gttggaagtt atagtatctc
      361 ccaaacgatt ggacttgact taggatggaa cattgtgtct ttttgtgtca tccctgaaaa
      421 tcccgatatg cttaatgttt ttcaaaatct tgtcaataat aatagcttga taaaaatcca
      481 gaatgaaacc ggtcaagcag ttgaaaaatt aggagcaatc ggctgggtca ataatatcgg
      541 caatcttacc gctacggaag gttattacgt taaagtagcc tcaaatcaaa cattgacaat
      601 tgaaggcaat ggtatcccac taccactcgg aatacctctt acttcaggtt ggaatattat
      661 gagctaccca gcttcatcag cccagaatgc cttggatgtt attcaaccat taatagatga
      721 cggcaaatta cagaaaatcc aggatgaagt cggcaatgcc attgagaacc taccgactat
      781 tggctgggtc aataacatcg gtaattttga accgggcgat ggttattaca tcaaagtcaa
      841 cagcacgacc tctgttacgg tggatcaacc gaccggttcc cctctggcta aatcccagat
      901 ggctacctta gctgtgtcac ctactcacta tctgccaata actgcgatga atccttatct
      961 gccgatgaat gtttatgtaa tgtcggctac cgtagaaggc gagcccctga aaaccggtgc
     1021 cgagatcgcc atttaccatc aggatcggtg tattacggcc gccgccttac cctatcctct
     1081 gaataccgaa aacgcctatc taccgctaat tatcggtgca gatgatcctc tcagtgaaga
     1141 aattgaagga tttaacgaaa acgatcctat ctccttcaaa atctgggatg ggaatgagga
     1201 actgttagta tcagttaaag tcataaaata tggcgataat ccgggaattc tcaaattcaa
     1261 cgctcacgga acaatggtcg ttgaattgga agcacagcgc attccgaaag aatacagttt
     1321 acaccagaat tatcccaatc ctttcaatcc gactacaact cttcgctatg aattacccaa
     1381 accgggaaaa gtagaaatcc gtattttcga tttgcaggga agattggtca atcaactggt
     1441 atccgaatac caggaggttg gtaaatattc cgtggtctgg aatggctgtg acaaccatgg
     1501 cattccggtg ccgagcggta tttatttcta tcagatcagt actggctcat atagtaaaac
     1561 ccgtaaagcc attctgatga aataagccag cctgaggtta tcatgaacaa aataagaaaa
     1621 tgcctgctaa ttttacttct ctgtaatatt caatttatgg tagcggcgcc ggaaaccaat
     1681 ccgacgcact tccagccagt ctatatcggc aatccctatc tggcgatgaa tatttacctg
     1741 accgttgtaa atattgatgg cg
//
LOCUS       DAOSMP010000179         1758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_67749_length_1758_cov_16.587199, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000179 DAOSMP010000000
VERSION     DAOSMP010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67749_length_1758_cov_16.587199"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PKX09_10805"
     CDS             complement(<1..512)
                     /locus_tag="PKX09_10805"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOU18350.1"
                     /translation="MIQNHEKHFPKKFLGNIITLIAGLVILVAMVAFGFFSQPREFEN
                     GDIVLDCLSQNVEILTGVDRIAHISAANDADLLRTIGYLQATRQLPNLDRILRLATGQ
                     MSAAHGKKFIELDIAAHQLDFVTLAEQTLTELSPEAQTLFEAYCAGINAFIATQRHSV
                     AQQFRLAGYEP"
     gene            618..911
                     /locus_tag="PKX09_10810"
     CDS             618..911
                     /locus_tag="PKX09_10810"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18351.1"
                     /translation="MSELIKKILLASVGAVSLTKEKAENLLEDLVKRGEIAREDKTSL
                     LNEILKSIEQSQQETRKFIQKEMEKALKALDIPTRSEFEELKKQVKSRQARKD"
     gene            920..>1758
                     /locus_tag="PKX09_10815"
     CDS             920..>1758
                     /locus_tag="PKX09_10815"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOU18352.1"
                     /translation="MRRFSVSRTWRHLKRYNQIISVLIKYGFSDVVDAARKDLIARFG
                     EKFVPRGRRNQFSSLSHAERIRRALEELGPTFVKIGQVLSLRPDIIPAEIAAELAKLQ
                     DEVSPLPYDVIANVIQTELGSEPDTVFQDIQSEPLAAASLAQVHKGVLISGEPVAIKV
                     QRPEAESLINTDIEILYDLASILQNFFLENFAIDILAFIREFDRSIHQELDFVLEGRN
                     LRRFRKFFAGDEAIFIPNYFAEYSTSKLLVMELVEGIKATEIEQLEAAGIDLQVIAQR
                     GVEA"
BASE COUNT          503 a          348 c          416 g          491 t
ORIGIN      
        1 ggttcataac cggccaggcg gaattgttgg gcaactgaat ggcgctgagt ggcgatgaaa
       61 gcatttattc ctgcgcaata agcctcaaaa agtgtctgtg cttcgggcga aagttctgtc
      121 agtgtctgtt cggctaaggt aacaaaatca agttggtggg cggcaatgtc caattcgata
      181 aattttttac catgtgcggc agacatctgg ccagtggcta agcgcaatat acgatccaga
      241 tttggcaatt gtcgggtggc ttggagatag ccaatagttc gcagaagatc agcatcatta
      301 gcggcactaa tatgggcaat gcgatccact ccggtgagaa tttccacatt ttgagaaagg
      361 caatctaaga cgatgtcacc attttcaaat tctctcggtt gactgaaaaa tccgaaagcg
      421 accatcgcga ccagaatgac caatccggcg attaaggtta tgatattgcc cagaaatttt
      481 ttaggaaaat gtttctcgtg attctgtatc attctacggt aaatataatt tattttaagg
      541 ggcgtgacaa gaagatgtaa aattgctttt acgcttgaag ttgattaaaa taatgttgta
      601 aaaaaggaga atgcattatg agtgagctga ttaaaaaaat tttgctggca agtgttggtg
      661 ctgtttcctt aaccaaggag aaagctgaaa acctgttgga agacctggtt aaacgcggtg
      721 aaattgctcg tgaagacaaa accagcctgc tgaatgaaat cttgaagtct attgagcaaa
      781 gtcaacagga aactcgcaaa tttattcaaa aagaaatgga gaaagccctg aaagctcttg
      841 acattccaac tcgttcggaa tttgaagagc tcaaaaaaca ggttaaatcc cgtcaggcgc
      901 gcaaagatta aatcccctga tgaggcgttt ttccgtttcc cgcacttggc gtcatctcaa
      961 acgctataat cagatcattt cggttttgat caagtacggt ttttctgatg tagtggatgc
     1021 cgcccgtaaa gatctgattg cacgtttcgg tgaaaaattt gtcccgcgcg gtcgccggaa
     1081 ccaattcagt agcctgtccc atgcagagcg aatccgtcga gcgttggaag aactcggtcc
     1141 aacttttgtc aagattgggc aggttttaag tctgcgtcca gatattattc cggctgaaat
     1201 tgccgccgaa cttgctaaac ttcaggacga agtttcaccg ctcccttatg atgttattgc
     1261 taatgtaatt cagactgaat taggaagcga acccgatacc gtttttcagg atattcaatc
     1321 agaaccgtta gcagccgcat ccctggctca ggttcataaa ggcgttctaa taagcggtga
     1381 accggttgct attaaagttc agcgacctga ggctgagtcg ctcatcaata ccgatattga
     1441 aattttatac gatttggcat ccattctaca aaactttttt ctggaaaact tcgctattga
     1501 tattttggct ttcatacgcg aatttgatcg ttcgattcac caggaactgg attttgtact
     1561 ggaaggtcga aatctgaggc gttttcggaa attttttgcc ggtgatgagg ctatctttat
     1621 tccaaattac ttcgcggagt atagtacatc gaagctatta gtgatggaac tggtagaagg
     1681 gattaaggct acagaaatcg aacaactgga agcggctggt atagatcttc aagtaatcgc
     1741 ccagcgcggc gtcgaagc
//
LOCUS       DAOSMP010000180         1749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_68271_length_1749_cov_13.345336, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000180 DAOSMP010000000
VERSION     DAOSMP010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68271_length_1749_cov_13.345336"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(425..718)
                     /locus_tag="PKX09_10820"
     CDS             complement(425..718)
                     /locus_tag="PKX09_10820"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU18353.1"
                     /translation="MGLDNSLKVPDRYALRSNYPNPFNPTTTIAYQLSETAFVRLAVY
                     NMNGQLVENLIAKEQSAGYYDTVWNATNYSSGIYLYKFQAGNFQQVGKCLLLK"
     gene            complement(1214..1749)
                     /locus_tag="PKX09_10825"
                     /pseudo
     CDS             complement(1214..1749)
                     /locus_tag="PKX09_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
BASE COUNT          489 a          405 c          363 g          492 t
ORIGIN      
        1 agcctgctgg ggaaattttg aggttaagcc tgtagttgag aaaatagatt acggggtacg
       61 ggagtgaaaa acttgttttt agctattata acgtaaagat tcacaaccct tttctatttt
      121 cctttacgtc tcttagcgaa cttagcggtt taatcttatg ccacaaagac ttgagaaaaa
      181 ggtcaagcca gagaaaagag aagcaaaaga gaaagggtat ggcttggctt aagttgccgt
      241 ccggcgattt gggttaatcc gaacttttaa agcgctttat ctcgaaagaa aacatcacgt
      301 atccaaaccc ttccccattc agttttaagt tgcctgacca tcggtaattt ttgaggaaag
      361 gtaaaaccaa atattctaca gccccaacaa tttgagatcg taaacagtca ggtattttga
      421 ggacttattt caataataaa catttcccga cctgttgaaa attgcctgcc tgaaatttat
      481 acaagtatat tcccgaacta taattcgtgg cgttccagac agtatcgtaa tacccggccg
      541 attgttcttt agcaatcagg ttttcgacta attgaccatt catattatat acagcaagtc
      601 tgacaaaggc tgtctcggac aattgataag cgatcgtcgt ggtgggatta aaaggattgg
      661 gataatttga tctgagagca tagcgatcgg gcactttgag tgaattatcc agtcccattc
      721 cggcaggact acaaatgttt tcggcctcgt ctcccatacc aatcggcgcg taggcggaat
      781 ttgaaagaca aattttatcc agctttgctc cgtcttcgcg gtaagtgatc gtaagtttat
      841 gctcgccttt cgtcagagtg taagaactta aattcttcca ttcccacccg gtcgtcacta
      901 atccgttaaa catttgaaaa ttaccaccat ccatttttac ccaataagaa tcatcatcat
      961 aggaggggca gtttaatctc gcatacagat aataacttcc ggcactatca accgaaaaag
     1021 gaatatgaat ggcgtttgtg tcaccggagg gcgcgctatc gagactttgc attccggctt
     1081 tgacggtcac atatttacca ttggaagcct gagagtcggt caatatatcc caattttctc
     1141 ctacgatagc acattcgggc tcgaaataaa ttctcatact ttcatcggta aaatccaagg
     1201 gttctctgcc tatgaacaaa tttagatcca ccgcttcggc tatcaaccga tgcccgattt
     1261 cattggggtg gaggtgatct ccagaatccg cttccggtaa tagacttaaa gtatctgccg
     1321 gattgcgcag tgcccgatct aaatctatga ctccatcgaa gaaaccgctg gttcttatcc
     1381 aatcattgac aatttttcgc tcggcttcat gagctgtaga ataataaaat gagcccttca
     1441 tgggtaataa cgttgctccg tacacaaaga tacctctgga atgcgcattt actatcatct
     1501 gcttgtaagc gtcaattagt ttgttgccca cgccgggcgc cccgttgcca atgtcattta
     1561 taccctcgaa aatgatcagc cacctcactc cgctttgatt gagtacatcg cgttcaaagc
     1621 gactaagcgc ggtcggtccc aagcctccac tgagcacgca attaccgcca attccctggt
     1681 tcaaaaccgc tacgaaacgg gtagccggat tttcctgaaa gcgacgcgcc agttcatctg
     1741 gccagcggt
//
LOCUS       DAOSMP010000181         1738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_68930_length_1738_cov_11.396316, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000181 DAOSMP010000000
VERSION     DAOSMP010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68930_length_1738_cov_11.396316"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="PKX09_10830"
     CDS             <1..1271
                     /locus_tag="PKX09_10830"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOU18354.1"
                     /translation="LDNQFGEIYWACKPDILVEMANYLNNNPEWRGKATGGADVTGGW
                     YNGMFQNLANSYRYREEYYAGYVMSEINFRNLMIVGGARYEKTESKYTAYNMYDMRNP
                     DAQECDTVYSSPKSEFWLPMVQAKYSPFEWFDIRYAYTQTLARPDYHQMSPKINYDNT
                     RRSVRAGNPDLVPAHAYNHDLNLTFHGNKLGLLSIGGFYKTIKDFVYSTAYILHKTSK
                     SSDIKTINDFQIMGIKPEEGATLYTYINSRYLAYVKGLEFDWQTRFWYLPWGLDGIVL
                     GVNYTMIESEATYPLREEVTDYSVRPPITTTRDSTRTGRLLYQPNDVVNAYIGYEYKG
                     FSARLSFLFQGNSVSYVGTFPEQDGYTRDYFRIDASVRQTLPWLGSEIYLDVNNLNNE
                     VNSSAQMSIDGFTNVKHYGMTANLGIRFRL"
     gene            1345..>1738
                     /locus_tag="PKX09_10835"
     CDS             1345..>1738
                     /locus_tag="PKX09_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18355.1"
                     /translation="MKRMLVFLLCAIFLTVGLNAQEKTVVEVNGIYSGGSEGSLNDAV
                     NTVLNNEGTLSNTVFKLKSNDWYVMTGVIEVPVGQVLELVGEDIGATQETSPPQMLWT
                     ASSSVNKTFMIAVYGDLIMKNIWVRFVDV"
BASE COUNT          524 a          303 c          388 g          523 t
ORIGIN      
        1 ttctggacaa tcaatttgga gaaatctact gggcttgtaa gccggatatt ctggtcgaaa
       61 tggctaacta tttgaataat aatccagaat ggagaggaaa agctactggt ggagccgacg
      121 taaccggagg ctggtataat ggaatgttcc agaatctagc taattcatat agatataggg
      181 aagagtatta tgccggttat gtaatgtcgg agattaattt caggaattta atgatagtcg
      241 gcggagcccg gtacgaaaaa accgagtcta aatataccgc ttacaatatg tacgatatgc
      301 gcaaccctga tgctcaggaa tgcgatacgg tctattcctc tccaaaaagc gaattctggt
      361 tgccaatggt tcaagctaaa tactcacctt tcgagtggtt cgatattcgt tatgcctaca
      421 ctcaaaccct tgctcggccg gattatcacc agatgtcgcc taaaataaac tatgataata
      481 ccagaaggag tgtgcgagcc ggtaatcccg atttagtacc ggcgcatgct tataatcacg
      541 atttgaactt gacctttcac ggcaataagt tgggtcttct ttcaatcggc ggtttttata
      601 aaaccataaa ggattttgtt tatagtacgg cttatatcct ccataagact tccaaatcaa
      661 gcgacattaa gacgattaat gattttcaaa ttatggggat taaaccggag gaaggagcta
      721 ctttatacac ttatattaat agtcgctatc tggcttatgt aaaaggtctc gaatttgact
      781 ggcaaaccag attctggtat ctaccatggg gtttggatgg aatagttctg ggtgttaact
      841 atacgatgat cgaatcagaa gccacttatc ctttgagaga ggaagtgact gattattccg
      901 ttagaccacc gataactacg actcgtgatt ccactcgtac cgggcgtttg ctctatcagc
      961 cgaatgatgt ggttaacgcc tacatcggtt atgaatataa aggcttttca gcccggttat
     1021 cgttcttgtt ccagggtaat tccgttagtt atgtaggtac ttttccggag caagacggat
     1081 acaccagaga ttatttccgg atagatgctt ccgttcggca gactctaccg tggcttggtt
     1141 cggaaattta tttggatgtc aataatctca ataatgaagt taattcatca gctcagatgt
     1201 ctattgatgg ctttactaat gtaaaacatt atggtatgac ggctaatctt gggatcagat
     1261 tcagattata agacaattat gaaaggaata tttttaaaaa taatgccaaa atactcgaaa
     1321 caaaaaattc aggaggtcaa aaacatgaaa agaatgttag tatttttact ttgtgcaatt
     1381 tttctgactg ttggactaaa tgcacaagag aagactgttg tagaagtaaa cggtatttat
     1441 agtggtggtt ctgaaggttc tctgaacgat gccgttaaca ctgttcttaa taatgaagga
     1501 acattatcta atactgtttt taaactaaaa tcgaatgact ggtacgttat gactggtgtt
     1561 attgaagtgc cggtagggca ggttctggaa cttgtcggcg aggatattgg agcaactcag
     1621 gaaacatcac cacctcaaat gctctggacg gcgagtagca gcgtaaacaa gacttttatg
     1681 attgcagtat atggtgattt gattatgaaa aatatctggg tcagatttgt agatgttg
//
LOCUS       DAOSMP010000182         1738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_68931_length_1738_cov_9.941771, whole genome shotgun sequence.
ACCESSION   DAOSMP010000182 DAOSMP010000000
VERSION     DAOSMP010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68931_length_1738_cov_9.941771"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="PKX09_10840"
     CDS             <1..1271
                     /locus_tag="PKX09_10840"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOU18356.1"
                     /translation="LDNQFGEIYWACKPDILVEMADYLNNNPQWRGKATGGADQTGGW
                     YNGMFQNLANSYRYREEYYAGYVMSEIDFRNLMIVGGARYEKTESKYTAYNMYDMRNP
                     DAQNCDTVYSYPKSEFWLPMVQAKYSPFEWFDIRYAYTQTLARPDYHQMSPKINYDNT
                     RRSVRAGNPDLVPAHAYNHDLNLTFHGNKLGLLSIGGFYKTIKDFVYSTAYILHKTSK
                     SSDIKTINDFQIMGIKPEEGATLYTYINSRYLAYVKGLEFDWQTRFWYLPWGLDGIVL
                     GVNYTMIESEATYPLREEVTDYTVRPPITTTRDSTRTGRLLYQPNDVVNAYIGYEYKG
                     FSARLSFLFQGNSVSYVGTFPEQDGYTRDYFRIDASVRQTLPWLGSEIYLDVNNLNNE
                     VNSSAQMSIDGFTNVKHYGMTANLGIRFRL"
     gene            1345..>1738
                     /locus_tag="PKX09_10845"
     CDS             1345..>1738
                     /locus_tag="PKX09_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18357.1"
                     /translation="MKRMLVFLLCAIFLTVGLNAQEKAVVEVNGIYGGGLEGSLNDAV
                     KTVLNTEGTLSNTVFKLKSNDWYVMTGVIEVPVGQVLELVGEDIGATQETSPPQMLWT
                     ASTSVNKTFMIAVYGDLIMKNIWVRFVDV"
BASE COUNT          523 a          306 c          390 g          519 t
ORIGIN      
        1 ttctggacaa tcaatttgga gaaatctact gggcttgtaa gccggatatt ctggtggaga
       61 tggcagacta tttgaataat aatccccaat ggagaggaaa agctaccggt ggagccgatc
      121 agaccggagg ttggtataat ggaatgttcc agaatttagc taattcatat agatataggg
      181 aagaatatta tgccggttat gtaatgtcgg agattgactt caggaattta atgatagttg
      241 gcggagcccg gtacgaaaaa accgagtcta aatataccgc ctacaatatg tacgatatgc
      301 gcaaccctga tgctcagaat tgcgatacag tctattctta tccaaaaagc gaattctggt
      361 tgccaatggt tcaagctaaa tactcacctt tcgagtggtt cgatattcgt tatgcctaca
      421 ctcaaaccct tgctcggccg gattatcacc agatgtcgcc taaaataaac tatgataata
      481 ccagaaggag tgtgcgagcc ggtaatcccg atttagtacc ggcgcatgct tataatcacg
      541 atttgaactt gacctttcac ggcaataagt tgggtcttct ttcaatcggc ggtttttata
      601 aaaccataaa ggattttgtt tatagtacgg cttatatcct ccataagact tccaaatcaa
      661 gcgacattaa gacgattaat gattttcaaa ttatggggat taaaccggag gaaggagcta
      721 ctttatacac ttatattaat agtcgctatc tggcttatgt aaaaggtctc gaatttgact
      781 ggcaaaccag attctggtat ctaccatggg gtttggatgg aatagttctg ggtgttaact
      841 atacgatgat cgaatcagaa gccacttatc ctttgagaga ggaagtgact gattataccg
      901 ttaggccgcc gataactacg actcgtgatt ccactcgtac cggacgtttg ctctatcagc
      961 cgaatgacgt ggttaacgcc tacatcggtt atgaatataa aggcttttca gcccggttat
     1021 cattcttgtt ccagggtaat tccgttagtt acgtaggtac ttttccggag caagatggat
     1081 acaccagaga ttatttccgg atagatgctt ccgttcgaca gactctacca tggcttggtt
     1141 cggaaattta tttggatgtc aataatctca ataatgaggt taattcatcg gctcagatgt
     1201 ccattgatgg ctttactaat gtaaaacatt atggtatgac ggctaatctt gggatcagat
     1261 tcagattata agacaattat gaaaggaata tttttaaaaa taatgccaaa atactcgaaa
     1321 caaaaaattc aggaggtcaa aaacatgaaa agaatgttag tatttttact ttgtgcaatt
     1381 tttctgactg taggactaaa tgcacaagag aaggctgttg tagaagtaaa cggtatttat
     1441 ggtggtggtt tagaaggttc tctgaacgat gccgttaaaa ctgttcttaa tactgaagga
     1501 acattatcca atactgtttt taaattaaaa tcgaatgact ggtacgttat gactggtgtt
     1561 attgaagtgc cggtcggaca ggttctggaa cttgtcggcg aggatattgg agcaactcag
     1621 gaaacatcac cgcctcagat gctctggacg gcgagtacca gcgtaaacaa gacttttatg
     1681 atcgcagtat atggtgattt gattatgaaa aatatctggg tcagatttgt agatgttg
//
LOCUS       DAOSMP010000183         1719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_69970_length_1719_cov_18.429087, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000183 DAOSMP010000000
VERSION     DAOSMP010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69970_length_1719_cov_18.429087"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1712
                     /locus_tag="PKX09_10850"
     CDS             141..1712
                     /locus_tag="PKX09_10850"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HOU18358.1"
                     /translation="MIKKRILILLLAIMTLVTMALPQYYISPYEKVYDDLLYLQAGGC
                     LRDLDLNHTPISTIQIAQSLKLQLKSDSYTTAGATQRLLIDRIAVAYAPADTDLVLNL
                     KRKLYDLLKIESGQSYLHYGVKINLDYQNQPQSKLYPQLRTFGSLSVTKNLSITNVMT
                     LDPYATDSPDSPTGNLDYIGKEWRGLSGYTEQAYLMWSAKVFRLTAGRSYIVFGPGRK
                     SSLLFSSAARPLDNLKFDIFLKHLSFQGITAKLDKMDDNERYLSAHRLSFYLGNWAIG
                     LTEAMLYTGINQNLEFAYLNPFLLYHGEQLNGPDLNGNTLGTLELSYTGRNWIAYSEF
                     LVDDIQFDKEEPNDLEPNEVGCILGMDIADPFKIPGVYLGMEYTAITNRTYKTLTPSE
                     WFLHRNVPIGYELGSDLDRFNVFVRKYHKNLQFALEFDHIRQGEGDLNKPWDSPWDTL
                     TVEQGYSEKFPTGKVQTTSDVGLEVTWVPDYGKYIYARLTYETIKNIDHGNKNAQNLI
                     FSVGAYLDLHGKIGF"
BASE COUNT          482 a          348 c          372 g          517 t
ORIGIN      
        1 ctaaattccg tgcagttggt gtctttcgta tttaataaat tcagcttgcg aagctgtgct
       61 tgattttcct ctttggtctt tcattttcag gcattaatat atgtcgtgta tccgataaat
      121 ttgtggaagg cagaaaaaag atgataaaaa aaagaatttt aatcttattg ctggctataa
      181 tgactttagt gactatggca ttgccacaat attatatttc gccttatgag aaggtttatg
      241 atgatctact ctatttacaa gccggtgggt gcttacgaga tctcgatttg aatcatactc
      301 cgatttcgac cattcaaatt gcgcaatcgc taaaacttca gttgaaatcg gatagttata
      361 ccacagccgg agcgactcaa cgtttgttga ttgataggat tgctgtggcg tatgctcccg
      421 ccgatactga ccttgtttta aatttaaaac gcaagctcta tgacctatta aagattgaaa
      481 gcggtcaatc atatttacac tatggagtca aaattaacct cgactaccag aatcaacctc
      541 aatccaaact ttatccccag cttcgtactt ttggttcgct atcggtgacc aaaaatctca
      601 gtatcaccaa tgttatgacg ctcgatccct acgccactga ctcgcctgac agtccgaccg
      661 gaaatctgga ttatatcgga aaggaatggc gcggtctgtc cggttataca gagcaggctt
      721 atttgatgtg gagtgctaaa gtattccgtc tgaccgccgg acgcagttat atagtttttg
      781 gtccgggacg caagagctca ctgctatttt cttcggcggc gcgcccatta gataatctaa
      841 aattcgatat ttttttgaaa cacctcagtt ttcagggaat cacagccaag ctcgataaga
      901 tggatgataa cgagcgttac ctttcagcgc accgcttgag tttctatctg ggaaattggg
      961 cgatcggctt gacggaagcg atgctatata ccggtattaa ccagaacctt gaattcgctt
     1021 acctgaatcc ttttctactt tatcacggtg aacaattaaa tggtcctgac ctgaatggca
     1081 atacactcgg gacactggaa ctcagttata ccggacggaa ctggattgct tatagtgaat
     1141 ttttagttga tgacattcaa ttcgataaag aagaacctaa tgatcttgaa ccgaatgaag
     1201 tcggttgtat tctggggatg gatattgctg atccgtttaa aattccgggc gtatatctcg
     1261 gaatggaata cacagccatt accaatcgca cctataaaac acttacgcca agcgagtggt
     1321 ttttacaccg taacgtgcca atcggttacg agctgggtag tgacttagat cgttttaatg
     1381 tgttcgttcg caagtatcat aagaacctgc aatttgcatt agagttcgac catattcggc
     1441 agggtgaagg tgatttgaat aaaccctggg attcgccttg ggataccctg actgtcgaac
     1501 aaggttactc ggagaaattt ccaaccggta aagttcaaac gactagtgac gtagggctgg
     1561 aggtaacctg ggtgccggat tacggtaaat atatttatgc acgtctaacc tacgaaacga
     1621 ttaaaaatat agatcatggt aataaaaacg cgcagaattt gattttttcc gttggagcct
     1681 acctggattt acatggtaag attggttttt aatttcccg
//
LOCUS       DAOSMP010000184         1715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_70177_length_1715_cov_14.262048, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000184 DAOSMP010000000
VERSION     DAOSMP010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70177_length_1715_cov_14.262048"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PKX09_10855"
     CDS             complement(<1..728)
                     /locus_tag="PKX09_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18359.1"
                     /translation="MRRLLILLAVPLIIQAQSIVGEWSSYTSIISHRELTQVGDYIYI
                     ASPGGLVEFNKTSSKFKVYSPNDGLSFTDINCLGLDELGDIWLGMSSPNGEVNIWSPV
                     TRQCKKVINAAELREELTLISAITFTADKAYVAYQKNITWGIMYFKIDKNEYTYIDFC
                     ENFPAEFFKINKLVAINDTLWVATDAALFFADLQENFPKGPNNWEVVDLIGQGNITSI
                     IEYDEEVICSCGNYIYGIKGTSAVV"
     gene            complement(732..1211)
                     /gene="ribE"
                     /locus_tag="PKX09_10860"
     CDS             complement(732..1211)
                     /gene="ribE"
                     /locus_tag="PKX09_10860"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOU18360.1"
                     /translation="MKLHEGVLSAEKMKVGIVASRFNDMITKELLAGALDCLKRHGVD
                     ENDIHVYWVPGALEIPRIARELADSGQFDGVVCLGAVIRGATPHFDIVANEASKGIAR
                     LSMESDVPVIFGVLTTENIEQALERAGTKAGNKGWDAALNMIEMANLIRSIRVHSQK"
     gene            complement(1207..>1715)
                     /gene="ribA"
                     /locus_tag="PKX09_10865"
     CDS             complement(1207..>1715)
                     /gene="ribA"
                     /locus_tag="PKX09_10865"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HOU18361.1"
                     /translation="VMGDIKSDEPVIVRVHSECLTGDVFSSERCDCGDQLHKALEIIA
                     QNKRGVLLYLRQEGRGIGLRHKISAYKLQDEGMDTVEANIKLGFLPDLRDYGMGAQIL
                     ADLGVHKMRLLTNNPKKIVGLQGFNLEVVERIPIEIPPNPQNEKYLKTKRDKMGHLIL
                     NNKKVPND"
BASE COUNT          482 a          439 c          311 g          483 t
ORIGIN      
        1 acaaccgctg aagtaccctt aatcccgtaa atataatttc cgcaggagca aatcacttcc
       61 tcgtcatatt cgattatact ggtaatgttc ccctgcccga ttaaatccac cacttcccaa
      121 ttattaggtc ctttaggaaa attctcctgc aaatcggcaa aaaacaaagc cgcatcggta
      181 gctacccata gcgtatcgtt aattgcaact aacttgttaa ttttaaaaaa ctctgccgga
      241 aaattttcgc aaaaatcaat gtaggtgtac tcgttcttat caattttaaa atacattatt
      301 ccccaggtga tatttttttg gtaagccaca taggctttgt ccgcggtgaa ggtgatcgct
      361 gaaataagag tcaattcttc gcgtaactcg gcggcattaa ttactttctt acactggcgc
      421 gtaactggac tccagatatt gacctcgcca tttggtgaac tcatccccaa ccagatatcg
      481 ccaagttcat cgagtcccag acaattaata tcggtaaatg ataaaccgtc attcgggcta
      541 tatactttga atttcgaact ggttttattg aactcgacta atccaccggg actagcaata
      601 tagatataat caccaacctg ggtcaattct ctatggctaa taatcgaagt gtaactactc
      661 cattccccca ctatgctctg agcttgaata atcagcggca cagctaaaag tattagcaat
      721 cgtcgcatag tttacttctg actatggaca cgaattgaac gaatcaaatt cgccatctca
      781 atcatattga gagcggcatc ccaacctttg ttaccggctt tcgtgccagc acgttcgaga
      841 gcttgctcga tattttccgt cgttaggaca ccgaaaatca ccggcacgtc tgattccatt
      901 gatagtcgag cgattccttt ggaagcctca ttggcaacga tatcaaaatg aggtgtcgcg
      961 ccacgaataa cggctcctag acaaacaaca ccgtcaaact ggccagaatc agctaattcg
     1021 cgagcaatgc gcggaatttc aagcgcgccc ggcacccagt aaacgtggat atcattttca
     1081 tcaacgccat gccgtttcaa acagtccagc gcaccggcaa gcaattcttt agtaatcata
     1141 tcgttaaacc gactggcaac gatcccgact ttcattttct cggcggaaag cacgccttcg
     1201 tgtagtttaa tcatttggca cctttttatt atttaagatt aaatgtccca ttttgtcccg
     1261 tttagttttg agatattttt cattttgggg atttggtgga atctcgatcg gaatgcgctc
     1321 cactacttcg agattaaatc cctgcaagcc aacaattttc tttggattat tggtcagcag
     1381 tcgcatctta tgcactccta gatcagccag aatttgggca cccatgccgt aatcacgcaa
     1441 atccggcaga aaacccaatt tgatattagc ctcgactgta tccatacctt catcctgcag
     1501 tttataagcc gaaattttat gacgtaaacc gatcccgcgt ccctcctgcc gtaaataaag
     1561 caagacaccg cgtttgttct gcgcaattat ctccagcgct ttgtgcaact gatcaccgca
     1621 atcacaccgc tctgagctaa aaacatcgcc agtcaggcat tcagaatgca cccgaacaat
     1681 caccggttca tccgatttta tatctcccat aacca
//
LOCUS       DAOSMP010000185         1701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_70985_length_1701_cov_12.985419, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000185 DAOSMP010000000
VERSION     DAOSMP010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70985_length_1701_cov_12.985419"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1701
                     /locus_tag="PKX09_10870"
     CDS             <1..>1701
                     /locus_tag="PKX09_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOU18362.1"
                     /translation="ILDCPKTMPALNNTKDSIVIRDHSAHLIDGLEYSSAWGYRQGVS
                     LERKNPFVSSNQASNWSLSTDLDKATPGRRNSIDLRDYDLALDSVKIKPGSSPPSHNS
                     PVTAQIYLHNNGKKLADAFKINIAITSSVLGTISDTTITISSPLQSGEIQTVAVTFNA
                     IPGGLHQISAEILWSADETLTNNIANCALSVGYPTGVLVINEIMFVPLAGQCEWFEIY
                     NRSEIALDLNGWSFRDAMGKRLALADSGIVIKVGEYAVVAARSDFTLDYPEFQELLIV
                     PTAFPTLNNTTDSLFLYDATDNIIDIICYRDSWGGGTGVSLERLDPAQPALNAANWGG
                     SLVTATPGRRNSIDLRDYDLALDSVKVKPGKSPFQHNSPVTAQIYLRNNGKKSVDAFK
                     INIAIINSFLGTVSDTTITISSALNSGESQMVAVTFRAIPGGLHKIAAEILWSADETL
                     TNNITNCILSVGYPTGLLVINEIMFVPLAGQCEWFEIYNRSAITLDLNGWKFRDAMGK
                     RLALADSGKTIEAGEYAVVAAHSDFTLDYPDYQELLIVPTAFPTLNNTTDSLFLYDAT
                     G"
BASE COUNT          438 a          398 c          387 g          478 t
ORIGIN      
        1 tatcctggat tgtcctaaga ctatgccggc gctgaataac accaaagatt ctattgtaat
       61 ccgagaccac agcgctcatc taattgatgg attagaatat tcgagcgcct ggggttatcg
      121 tcagggcgtc tcacttgaga gaaaaaatcc atttgtgagt agcaatcaag ccagtaactg
      181 gtcgctctca acagaccttg ataaagctac tcccggtcgg agaaattcaa ttgacttacg
      241 ggattatgat cttgcccttg attccgttaa aataaaaccg ggcagttcac cgccgagcca
      301 caattcacca gttaccgcac aaatttacct tcataataac ggtaaaaaac ttgccgacgc
      361 atttaaaatc aatatcgcga taactagttc ggttttaggg acgatttccg atacaactat
      421 cacgatttct tctcccttgc agtcgggtga aatccaaaca gttgccgtta ctttcaatgc
      481 tattccggga ggcttgcacc aaatttcggc cgagattctg tggtcggcag atgagacact
      541 gactaataat attgccaatt gcgcactgtc ggttggctat ccgactggcg ttttagtcat
      601 taatgaaatt atgtttgtcc cacttgctgg tcaatgcgag tggttcgaaa tctataaccg
      661 ttcagaaata gcacttgatc tgaatggctg gagttttcgc gatgcaatgg gtaagcgcct
      721 ggctttggct gattcgggca tagtgattaa agtcggcgaa tatgccgtcg ttgcggcgcg
      781 gtctgatttt accctggact atccggaatt tcaggaactg ctgatagtgc cgaccgcttt
      841 tcctactctg aataacacga cggattcgct atttctctat gatgctaccg ataatattat
      901 tgatattatt tgttatcggg acagctgggg cggcggaaca ggcgtctcgc tggaacgact
      961 tgatccagcg caaccagctt tgaatgccgc taattggggc ggttcgctag tgacagctac
     1021 gcccggtcgg agaaattcaa tagacttgcg ggattatgat ctcgcactcg attcggttaa
     1081 agtaaaacct ggcaaatctc cgtttcaaca caattcaccg gtcaccgctc aaatttacct
     1141 gcgcaataac ggtaaaaaat ctgtcgacgc atttaaaatc aatatcgcca taattaattc
     1201 gttcttgggg acagtttccg atacaactat cacaatttct tctgctttga actcaggaga
     1261 aagccagatg gtcgccgtta ctttccgcgc tattccgggt ggcttgcaca aaattgcggc
     1321 cgaaattctg tggtcggcag atgagacgtt gactaataat atcaccaatt gcatactgtc
     1381 ggttggctat ccgactggcc ttttagtcat taatgaaatt atgtttgtcc cacttgccgg
     1441 ccaatgcgaa tggttcgaaa tctataaccg ttcagcaatc acgctcgacc taaatggctg
     1501 gaaatttcgc gatgcaatgg gtaaacgcct ggctttggct gattcgggga aaactattga
     1561 agctggcgaa tatgcggtcg tggcggcgca ttccgacttt accctggact atccggacta
     1621 tcaggaactg ctgattgtgc cgaccgcttt tcctactctg aataacacaa ctgattcact
     1681 tttcctctat gatgctaccg g
//
LOCUS       DAOSMP010000186         1667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_72989_length_1667_cov_9.814516, whole genome shotgun sequence.
ACCESSION   DAOSMP010000186 DAOSMP010000000
VERSION     DAOSMP010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72989_length_1667_cov_9.814516"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PKX09_10875"
     CDS             complement(<1..202)
                     /locus_tag="PKX09_10875"
                     /inference="COORDINATES: protein motif:HMM:NF026853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvaI/BcnI family restriction endonuclease"
                     /protein_id="HOU18363.1"
                     /translation="MNIAEWSTYVIAGKFMTKFDRLVLIIADNKIKNGKEYFHFNEAY
                     LLENPTPERFLLAFEKNELMIDL"
     gene            complement(946..>1667)
                     /locus_tag="PKX09_10880"
     CDS             complement(946..>1667)
                     /locus_tag="PKX09_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOU18364.1"
                     /translation="TCGKCKVMTSQERVNIMVKGLGDMCRTMDVFIIGGGFSTSFSDI
                     VPAVVVSVIGKLVTEKPLTKPAKNVGDKIIIIGVTGNDGNDTLFRAGLVEEMRPAIAL
                     FKEEKTAMEAALAAFATGKINACSDLGAAGIGAAICESARYGRRGAKVDLSQVPVSVK
                     DITPQEILICETQARYVVQVAPESVDEVLKAIRTKTEHVAVIGEITDTQQEVFEYKGE
                     IIATIPNQPSPDTLAILKSGK"
BASE COUNT          473 a          347 c          273 g          574 t
ORIGIN      
        1 gtaaatcaat catcaattca tttttttcaa atgctaataa aaacctttct ggagtaggat
       61 tttccaatag ataagcctca ttaaagtgga aatattcttt tccattcttt attttattgt
      121 ctgcaattat taaaaccagc ctatcaaact tagtcatgaa tttacctgca attacatagg
      181 tgctccactc agcaatattc acctcttcat agatgttttt aagatgatca agtgcctttc
      241 aggatctgaa atgaggtaaa atccctgtgt atttggtgcc tttttattaa ctcttttaat
      301 tgcatatcaa ttctttgaca taataactat atattcattt ttcattgttg aggctgtcct
      361 gccgacgata ttagaagggc tatttttaaa gggcattctt ttattaggta tatttctaaa
      421 atcccagcta tcatccacaa tctttgcatt tcgagaatat atctttactt gatagtctaa
      481 atcgggttca tttacgattt ttactacctg tttattttta tcaacatttc ccattatatt
      541 aaagctagtt agaccttata attttatatt tctcataatt atggattgtg tgcaaccatt
      601 cattataatt gagctctttt accacctttt tctatttata caaacaattt taatcattta
      661 ttaaaaatta agtcatcaaa agtaatttgt cgtaatttgt caatgctaat tatagatcaa
      721 gctatttaat aattttctgc ctacttagag tatcttctaa tgaattttat tttgtaatca
      781 tacttatcac ctaaccaagt ttaagattca agataaattg tatctttcga cctggctcat
      841 attcagatat tgactttaac agtccaagat gataatccag aatttccgac tttatcttta
      901 taaagccggt tactctattt atctaaattt ctaactaatc tttatttact tgccgctttt
      961 caaaatcgct aaagtgtcag gcgacggttg attgggaatc gtggcaataa tctcaccttt
     1021 gtattcgaaa acttcctgct gggtatcggt gatttctccg attacggcga cgtgctcggt
     1081 tttggtacga atggctttta gcacttcatc tactgattcc ggcgcaacct gaacgacata
     1141 gcgtgcctgg gtctcgcaaa ttaatatttc ctgcggagta atatctttaa cggaaaccgg
     1201 cacctgggac aaatcaactt tagcaccgcg ccggccgtaa cgggctgatt cgcaaattgc
     1261 cgcaccaatg ccggcggcac ccagatcaga acaggcatta atcttgccag tcgcaaaagc
     1321 cgccaaggcg gcttccatcg cggttttttc ttctttaaat agggcaatgg cagggcgcat
     1381 ttcttctacc aatcccgcac ggaaaagggt gtcgttgccg tcattgccgg tcacgccgat
     1441 aatgataatt ttatcaccga catttttggc cggtttggta agtggttttt cggtaaccag
     1501 tttgccgata accgaaacaa cgacagccgg tacgatatcg gagaaagaag tagaaaaacc
     1561 gccgccaatg ataaaaacat ccatcgttcg gcacatatct cccaaacctt tgaccataat
     1621 attcactctt tcctgactgg tcataacctt acatttgcca caggtgc
//
LOCUS       DAOSMP010000187         1663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_73244_length_1663_cov_10.228234, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000187 DAOSMP010000000
VERSION     DAOSMP010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73244_length_1663_cov_10.228234"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PKX09_10885"
     CDS             complement(<1..685)
                     /locus_tag="PKX09_10885"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOU18365.1"
                     /translation="MKIFRPYIFFLLFLVNCHNLLADQLVIQSPRPKTGTSISVLYFD
                     NCAYFSVKDFAQALGLRTYNNPETGKIVLYFSGKSVKVTAHSAFIMLDTRILQMSQPA
                     ILVNRDEIFVPLYSFLDLMKRYVIPKLQYYLDKRSPIYELVELPGATPTPSTPMVTTF
                     GTKPGIIYDIQFEERGNGLTMKISTNATYESSDFSSFFRGDEWFYLTILGATCDSAAL
                     SRRDPIPSVA"
     gene            complement(714..>1663)
                     /locus_tag="PKX09_10890"
     CDS             complement(714..>1663)
                     /locus_tag="PKX09_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18366.1"
                     /translation="LGEWDQGSAGILVYNANYSTGLFALNGHRKVSGISFLHNYEISD
                     WTISGEWAVQETGKKGIIQNLHLNRDRVSFCAGWRYIEPDFFAPLGNPFRKFSSLSGN
                     ETGLYSALKIKLPARWRFTGYVDFFRELKNSEPGVAIRNGRESLCGFSRQARNNDLIE
                     AWYKVNRYYHTSLPQQPRDYQLKLHLRHSLSASLSGEIRTTFRWNDSIPFTKGSQALS
                     VVGKVSISKNTRVTAGNTHYFTGSSDLNIYFYEPGLPFRFNMTLLTGTGQHYFLIISQ
                     RIGSNCELAGAVYGRNNASIPTNDNNFDFSGDFQLTFDL"
BASE COUNT          515 a          342 c          374 g          432 t
ORIGIN      
        1 ttgccactga aggaatcgga tcccgacgtg aaagcgcggc tgaatcgcag gtagcaccga
       61 gaatagtaag atagaaccac tcatcaccgc ggaaaaaaga agaaaagtct gaactttcgt
      121 aagtagcatt ggttgaaatt ttcatcgtca agccgttgcc gcgctcttca aactggatgt
      181 cgtaaatgat acccggcttt gtcccaaaag ttgttaccat aggcgttgat ggagtaggag
      241 ttgctcccgg caattctacc agctcataaa tcggtgagcg tttgtcgaga taatattgaa
      301 gtttagggat gacataccgc ttcatcaagt caagaaatga gtagagtgga acaaaaatct
      361 cgtcacggtt gaccaaaata gccggctgag acatttgcag tattcgagta tcaagcataa
      421 tgaaagcgct atgagccgtg accttgaccg atttaccgga aaaatagaga actatcttgc
      481 ctgtttcggg attgttataa gttcttaatc cgagcgcctg agcgaagtct tttacggaaa
      541 aataagcaca attatcaaag tagagaaccg atatactcgt acctgttttc ggacgaggag
      601 attgaatgac aagctgatcc gctaataggt tatgacaatt aaccaaaaat aatagaaaaa
      661 atatataagg tctaaatatt ttcacaatcg tcgtgaaatt acacacttct aggtcataag
      721 tcaaaggtca attgaaaatc acctgaaaaa tcaaaattgt tgtcattagt aggaatagaa
      781 gcgttgttcc gtccatagac tgcgccggct aattcacaat tcgaaccgat tcgctgactg
      841 attatcagaa aatagtgctg accagtgccg gttaatagag tcatattaaa gcggaaaggc
      901 aagccaggtt cataaaaata aatatttagg tcggacgaac cggtaaaata gtgggtattc
      961 ccggcggtca cacgggtatt tttagaaatc gagacttttc ccacaacgct aagtgcctgg
     1021 ctacctttag tgaaaggaat tgaatcgttc catctgaaag ttgttctgat ttcaccggaa
     1081 agcgaagcgc tcaaggaatg cctgaggtgt aatttaagct gataatcacg cggttgctgt
     1141 ggcagactgg tgtgatagta gcgattaact ttataccaag cttcaattaa gtcgttatta
     1201 cgagcttgac ggctgaatcc gcagagggat tcccgcccat tccggattgc tacaccaggc
     1261 tcggaatttt ttaattcacg aaagaaatca acatagccag tgaaccgcca gcgagccggt
     1321 agtttgattt tcagagcgga atataaaccg gtttcattgc cggaaagact ggagaacttg
     1381 cgaaaaggat tgccgagcgg agcgaaaaag tccggttcga tatagcgcca gcctgcacaa
     1441 aaacttacac gatctctatt tagatgaaga ttttgaatta tgcctttttt accggtttcc
     1501 tggactgccc actcaccgct tatcgtccag tcgcttattt cgtaattatg taagaatgat
     1561 atcccgctaa ctttgcgatg tccgtttaag gcaaataaac cagtcgagta attagcatta
     1621 tagaccaaaa ttccagccga gccttgatcc cactcgccca gca
//
LOCUS       DAOSMP010000188         1662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_73315_length_1662_cov_14.295582, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000188 DAOSMP010000000
VERSION     DAOSMP010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73315_length_1662_cov_14.295582"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /locus_tag="PKX09_10895"
     CDS             complement(<1..1464)
                     /locus_tag="PKX09_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOU18367.1"
                     /translation="MPTSDKLKAERRKWEETTLKKVISRNPERKTEFTTGSWLPVERT
                     ALPEITSDDEYLEKLGFPGEFPFTRGVQPTMYRGRLWTMRQYAGFGTAEESNRRYKFL
                     LNQGQTGLSIAFDLPTQIGYDSDDPHSEGEVGKVGVAIDTLRDMEILFDGIPLDKVST
                     SMTINAPAAILLAMYLAVAEKQGVAFDQLNGTIQNDILKEYIARGTYIFPPKPSLRLI
                     TNIFEFCARSVPKWNTISISGYHIREAGATAVQEVAFTLADGVEYVKTALNAGMDVDS
                     FADRLSFFFNAHNDLLEEVAKFRAARRIWAKIMRDKFHAQNSKSLMLRFHTQTAGSTL
                     TAQQPDNNIVRVAIQTLAAVLGGTQSLHTNSRDEALALPSEDSVRIALRTQQIVAYES
                     GVTNSVDPLGGSYLIENLTNQIESEVWQYLDQIEAVGGMIPAIEKGFIQKEIQDAAYR
                     YQKEIESNERIIIGVNKFTIDEETPKNLLKVDSELQRI"
BASE COUNT          431 a          346 c          426 g          459 t
ORIGIN      
        1 gatgcgctga agttcggaat caacttttaa taaatttttc ggtgtttctt cgtcgatggt
       61 gaatttattg acgccgatga tgatgcgctc gttgctttcg atttctttct ggtagcgata
      121 agccgcatcc tggatttctt tctggataaa gcccttttca atagctggaa tcatcccgcc
      181 aacggcttca atttgatcga gatattgcca gacttcgctt tcgatctgat tggtaagatt
      241 ctcaatcaaa tacgagccgc ccagcggatc aactgagtta gttacgccgg attcgtaggc
      301 gacaatttgt tgagtgcgta gagcaatgcg tacggaatct tcagacggta aagccagagc
      361 ctcatcgcgg gaattggtat gcagactctg agtgccgcca agcactgccg ccaatgtttg
      421 gattgcaacc cggacgatat tattgtccgg ctgttgggcg gtgagagtac taccggcagt
      481 ttgagtatga aagcgcaaca ttagagactt cgagttttgg gcgtggaatt tatcgcgcat
      541 aattttagcc cagatgcgcc gtgccgcgcg gaatttggcg acttcttcta ataagtcatt
      601 atgagcatta aagaaaaagg agagccggtc ggcaaaacta tcaacgtcca ttccggcgtt
      661 caaagcggtt ttgacgtatt caacgccatc agccagagtg aaagccactt cctggacggc
      721 agtcgcgccg gcttcgcgaa tatgatagcc ggaaatgctg atcgtattcc atttcgggac
      781 tgaacgagcg caaaattcaa aaatgttagt aattaaacgt agagacggct tgggcggaaa
      841 aatataggtg ccgcgggcta tgtattcttt cagaatgtcg ttctggattg tgccattgag
      901 ctggtcgaag gcgacacctt gtttttcggc tactgctaag tacattgcca gtaagattgc
      961 ggcgggagcg ttaattgtca tcgaagtgga aactttatct aaaggtatgc cgtcgaaaag
     1021 gatctccata tcgcgcagag tatcaatcgc tacgccgact ttgccaactt cgccttcgct
     1081 atgcggatcg tcggaatcat agccaatttg agtcggcaaa tcgaaggcga ttgataagcc
     1141 ggtttgtccc tgattaagca gaaatttata acgccgatta gattcttcgg cagttccgaa
     1201 tccggcgtac tgtcgcattg tccagagacg accgcgatac atcgtaggct gaacgccgcg
     1261 cgtgaagggg aactcaccgg gaaagccgag tttttccaga tattcatcgt cggaagtaat
     1321 ttccggcaga gccgtgcgtt cgaccggtaa ccatgagccg gtggtgaatt ccgtttttct
     1381 ttcaggattg cgagagataa ctttttttag agtagtctcc tcccactttc ggcgctctgc
     1441 ttttaattta tcggaggttg gcatagaatg ctcctgatta attattattt acactggtta
     1501 tggtatagag aagaaatgat tgtattgtga gcgacattaa ggttacatct cgctgtcaaa
     1561 ttgacttaaa aaattaaaga ggaatcagga gaaaaacaag atggctggca agattggaag
     1621 ttgggtaaat tatttgggct attaaagcga gaaattaaaa gc
//
LOCUS       DAOSMP010000189         1603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_77047_length_1603_cov_8.698320, whole genome shotgun sequence.
ACCESSION   DAOSMP010000189 DAOSMP010000000
VERSION     DAOSMP010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77047_length_1603_cov_8.698320"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1603
                     /locus_tag="PKX09_10900"
     CDS             <1..>1603
                     /locus_tag="PKX09_10900"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOU18368.1"
                     /translation="KNGTGKSYFGEGCIATDPLFKDYNNNDFTLNYNSPAIDAGNPDL
                     DADGTSWTDDVDDQDPDGTRLDMGAYYFVRTGIQGTITINGGSGKVEDAVIKATLTTA
                     PSTSSTTKPDKDGKYFLQLSNGSYNITIELAGTLAGYSSEPSVRNVVISDKLEKNIDF
                     TLNAPPPGSIKGNVTLEGIESVTSVTITAINISTGNSNSSHPNNYGYYTIYDLPAGTY
                     RVTAALTGYETAVDTISVTSGAPTTVDFSLKYVVVEGTISGKVSLKVDGGLLTNVKIT
                     VSDDEKTYHFTDKVNTAGDYEVTVNQGVYDVTASLAGYAPVTINNVQVVANNVTPNID
                     MRLINWSKITGTEFNMILFTTVSLEGDFFTKTKSDQLAAFGSGGINDCRGIATWKEDN
                     HPYWDNSWDIAGYWYLTIVSNNNTDDTISFKIFDTETDSVYDCNEKIIFTDCQIKDAI
                     LTIDGGAKEQKFDLVKEWNWVSFNLSPREASIDSVFKDLTEVSKIYQVKTQGSSATYT
                     NGSLVGDLTTVNNSDGYLIKMTDAF"
BASE COUNT          503 a          284 c          356 g          460 t
ORIGIN      
        1 ttaaaaatgg taccggaaaa agttactttg gtgaaggatg tattgctaca gacccgcttt
       61 ttaaagatta caataacaat gattttactc ttaattacaa ttccccagct attgatgctg
      121 gcaatcctga cctcgatgct gatggcacct cctggacgga tgatgtagat gatcaggacc
      181 cagacgggac caggttagat atgggagcat attatttcgt tcgcaccggg attcagggaa
      241 cgattactat taatggtggc agcgggaagg tagaagatgc ggttataaaa gcaactctga
      301 ctacggctcc ttcgacttca tctaccacca agcctgataa agacggcaaa tattttctgc
      361 agttatctaa tggaagttat aatataacta ttgaattagc gggaacttta gcaggttatt
      421 cttctgaacc gtcggtaagg aatgttgtga tttcagataa attggagaaa aatattgact
      481 ttaccttgaa tgcaccacct cccggttcaa ttaaggggaa tgtaactctg gaaggtattg
      541 aaagtgtaac ttcagttact ataactgcca taaatatcag taccgggaat tcaaattcaa
      601 gccatccaaa taattatggc tattatacca tatatgattt accggccggt acctatagag
      661 tcacagccgc gttaaccgga tacgaaaccg ccgtagatac aatcagtgta acctcaggtg
      721 ctcccacgac ggttgatttc agtctgaaat atgtagtcgt tgaaggcaca atcagcggga
      781 aggtttccct taaagtagac ggtggcttat taacgaatgt aaaaataacg gtttcagacg
      841 atgaaaaaac ttatcatttt acggataagg ttaataccgc gggtgactat gaggttactg
      901 taaatcaggg cgtttacgat gtcacggctt ccctggcagg ctatgctccg gtgacgatca
      961 ataatgttca ggtggtagcc aataatgtca ctccgaatat tgatatgcgg ctgattaact
     1021 ggagtaaaat taccggaact gaattcaata tgatcctatt tacgacagta tctttggaag
     1081 gtgatttttt taccaaaacc aaaagtgacc aattagccgc cttcgggagc ggcggcatta
     1141 atgactgtcg gggaatagca acttggaaag aagataatca tccttattgg gataactcct
     1201 gggatattgc aggctattgg tatctgacga ttgttagtaa taataacaca gatgatacga
     1261 ttagctttaa gatttttgat accgagaccg atagcgttta tgattgcaat gaaaagatta
     1321 tttttacaga ttgccagatt aaagatgcca ttttgactat tgatggaggc gctaaggagc
     1381 agaaattcga tttagttaaa gagtggaatt gggtatcttt taatttatca ccaagagaag
     1441 catccatcga ttcggtattt aaagacctta ccgaggtatc taaaatttat caggtaaaaa
     1501 ctcaaggtag ttcagcaaca tatacgaatg ggagtttggt tggtgattta accactgtca
     1561 acaatagtga cggctatttg ataaaaatga ccgatgcatt taa
//
LOCUS       DAOSMP010000190         1569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_79425_length_1569_cov_11.044254, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000190 DAOSMP010000000
VERSION     DAOSMP010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79425_length_1569_cov_11.044254"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1352)
                     /locus_tag="PKX09_10905"
     CDS             complement(102..1352)
                     /locus_tag="PKX09_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOU18369.1"
                     /translation="MVKIKPFRGIRPVKELADKIAAPPYDVISYEEARSLAADNPYSF
                     LHVSRAEIDLPPETDPYDDKVYQKARENLDAMLAKGWLFQDATEMLYIYRQIMDKHSQ
                     YGLITTANVNDYLKGKIKIHELTRQDKELDRTKHLEHTKVNTGPVFLTYQAKPVIDIM
                     IHHIVTDSQPEYDFTAMDGIQHTFWLVNDRETILKLVNHFSQLSHCYIADGHHRAKSA
                     ALVAKMRRERNPHHTGNEEYNWFLAAFFPHNQLRTLEYNRTVKDLNNLTEEEFHTKVG
                     EKFIIKPAECAICAKPSAPNQFAMYLNKRWYILIARPGTFDATDPVASLDVAILQNNL
                     LKPVLGIDDPRTDKRLDFSGGSRGMAELEKRVDSGEMAVAFALYPVSIEQLMAIADAG
                     KIMPPKSTWFEPKLRSGLVLHSLD"
     gene            complement(1361..>1569)
                     /locus_tag="PKX09_10910"
     CDS             complement(1361..>1569)
                     /locus_tag="PKX09_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU18370.1"
                     /translation="KALLAGLNSGQVGGAGLDVFAAEPPQNLELLHHARVSSTPHIGA
                     STLEAQERVGAEIAHRIVEYIGTL"
BASE COUNT          398 a          332 c          392 g          447 t
ORIGIN      
        1 aatagcggtc gaatttacag cactgattac gaaatgcaaa tgatggcatg tttgcgcttt
       61 aatcggcgag tgatagaacg ggcaaaatag gaataaaaat tttaatccag cgaatggagc
      121 actaatccgc ttcttaattt gggttcgaac caggtagatt tcggcggcat aattttccca
      181 gcatcggcaa tcgccataag ttgttcaatt gagactgggt aaagagcaaa tgccacagcc
      241 atttcaccgg aatcaacgcg tttttcgagt tctgccatgc cgcggcttcc gccgctaaaa
      301 tcgaggcgtt tatcggtacg cggatcgtca attccaagca ccggcttgag cagattattt
      361 tgcaaaatcg ccacgtctaa gctggcaacc ggatcagtgg catcgaaagt tccgggtctt
      421 gcaatgagga tataccatcg cttgtttagg tacatagcga actggttggg tgcggaaggc
      481 ttggcgcaga tggcacattc ggctggtttg attatgaatt tctctccgac tttcgtatga
      541 aattcctctt cagtcaaatt atttaaatcc tttacagtgc gattatattc gagagtgcga
      601 agctggttat gcgggaaaaa ggcggctaaa aaccagttat attcttcatt gccagtgtgg
      661 tgcggattac gctcgcgccg catcttcgcg actaaagcgg ctgatttggc gcgatgatga
      721 ccatcagcaa tatagcaatg ggagagttga ctgaaatggt tgactagttt gaggatggtt
      781 tcgcggtcgt tgaccagcca aaatgtatgc tggataccgt ccatagcagt aaaatcgtat
      841 tctggctggc tatcagttac aatatggtgg atcatgatgt caatgaccgg tttagcctgg
      901 taagtcaaaa ataccggacc ggtattgacc ttagtatgtt cgagatgctt ggttcgatcc
      961 agttccttat cttggcgagt caattcgtgg attttgattt ttcccttaag atagtcattg
     1021 acgttagcgg tggtaatcaa tccatactgg ctatgtttat ccattatctg gcgatagata
     1081 tatagcattt cggtcgcatc ctgaaagagc cagcctttgg caagcattgc atctaaattt
     1141 tcgcgagctt tctgatagac tttgtcatca tagggatcgg tttccggtgg taaatctatt
     1201 tccgccctgc tgacatgcag aaaagaatat ggattgtcgg cggcaagact tcgggcttct
     1261 tcataactta tgacatcata aggaggggcg gcgattttgt ccgctaattc tttaactgga
     1321 cggatgccac gaaaaggttt gatttttacc atataaattc ctacagagtt ccgatatact
     1381 ccacgattcg gtgagcaatt tcggcgccga cgcgttcttg agcttccaga gtacttgcgc
     1441 caatatgggg ggtgctggag acacgcgcat ggtgaagtaa ttccaggttt tgcggtggtt
     1501 ctgcggcaaa aacatccaga ccggcgccgc cgacttgacc agaattcaaa cctgctaaca
     1561 gtgcctttt
//
LOCUS       DAOSMP010000191         1560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_80059_length_1560_cov_16.413953, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000191 DAOSMP010000000
VERSION     DAOSMP010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80059_length_1560_cov_16.413953"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PKX09_10915"
     CDS             <1..548
                     /locus_tag="PKX09_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOU18371.1"
                     /translation="YIELGEISTYIPGYKENAGIFCHNNPWIMIAETKLGNGEQAFEY
                     WRKICPSFLEDISDLHRTEPYVYAQMIAGKDAYKPGEAKNSWLTGTAAWNFLAVSQYI
                     LGIRPQFDGLLIDPCIPSDWKSYTVRRLFRGKNLNIEVRNPAGVQKGVRRITLNGEAL
                     SNNLIPCSKLKNTNEVIVDMG"
     gene            651..>1560
                     /locus_tag="PKX09_10920"
     CDS             651..>1560
                     /locus_tag="PKX09_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HOU18372.1"
                     /translation="MAKYLTLREKIAYGFGDLASVLYWQTFMLYFTYFYTDVFLIPAG
                     VAATMFLVSRVWDAVNDPLMGIIADRTNTRWGKFRPYLLWFCVPLAVAGVLTFSVPNF
                     GMTGKIVYAYVTFILIMMLYTAINIPYTALLGVISPDSDERTSVSSIKFLFAFTAGVI
                     VSATLLPMVKALGKGNEARGWQLSFVIYGIAAIIFFLIAFKGTRERVQPPKSQKSSIK
                     KDLYELVTNSPWLILLFTTITFILFVAIRSSVTVHYFKYIVGTREVSLPFFKNKTWDF
                     ETLTSVFNTVGQISSFIGVLLVSWFAK"
BASE COUNT          415 a          278 c          368 g          499 t
ORIGIN      
        1 attacatcga attaggtgag atttcgactt acattcctgg ttataaggaa aacgccggaa
       61 tattttgcca taacaatccc tggattatga tcgctgaaac taaattaggt aacggtgagc
      121 aggcatttga atattggcgt aaaatctgtc cgtcgtttct cgaggatatc agcgatttac
      181 atcgtactga gccgtatgtc tatgctcaga tgattgccgg taaggatgcc tataaaccgg
      241 gggaagctaa gaattcctgg ctgaccggca cggcggcttg gaattttctg gcggtctcgc
      301 aatatattct gggaattcga ccacagtttg acggtttgct gattgatccc tgcataccat
      361 ccgactggaa aagctatacg gtgcgccgtc tattccgggg gaaaaattta aatattgaag
      421 taagaaatcc tgccggagtg cagaaaggtg tgcggcggat aactttaaac ggtgaagcat
      481 tgagtaataa tctaattcct tgttctaagc tgaaaaatac caatgaagta atcgtagata
      541 tgggttaaaa atttaaagat aaaactgtct gtttaggggg ataatagact ctttataatg
      601 gagtgaaatt aagatattac ggattaaaat tttaaaaaaa ggagaggaca atggctaagt
      661 atcttacctt acgggaaaaa atcgcatatg gttttggtga tctggcgtcg gtgctttact
      721 ggcagacttt tatgctatat ttcacttatt tttacacgga tgttttcttg attccagccg
      781 gagtagcggc tacgatgttt cttgtcagtc gggtctggga tgccgttaac gatccgttga
      841 tgggaattat agctgatcgc accaacaccc gctgggggaa attcagacca tatttattgt
      901 ggttttgtgt accgcttgcg gtggctggtg ttctgacatt cagcgtgcct aactttggga
      961 tgaccggtaa aatcgtttat gcttatgtga cttttatcct gattatgatg ctttatacgg
     1021 cgatcaatat tccatacacc gcattgctgg gagtaatttc gccagattcg gacgaacgca
     1081 catccgtttc atcgatcaaa tttttgtttg cgtttacggc tggggttatt gtgtcagcga
     1141 cgcttctgcc gatggtgaaa gctctcggta aaggcaacga agcgcgtggt tggcagttat
     1201 catttgtgat ttatggaatt gcggctatta tctttttcct gattgctttc aaaggcacgc
     1261 gcgagcgtgt tcagccgcca aagtcgcaaa aatcctcaat aaaaaaggat ttatatgaat
     1321 tagtaacaaa ttcgccctgg ttgatattgc tttttacgac tataacattt attctattcg
     1381 ttgccattag aagcagtgtg acagttcatt attttaaata tatcgtcgga acgcgtgagg
     1441 tttctttgcc gttttttaaa aataaaacct gggatttcga aacacttact tcggttttta
     1501 atactgttgg tcagatttcg tcatttattg gggttttgtt agttagctgg tttgccaaaa
//
LOCUS       DAOSMP010000192         1560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_80060_length_1560_cov_13.904983, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000192 DAOSMP010000000
VERSION     DAOSMP010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80060_length_1560_cov_13.904983"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PKX09_10925"
     CDS             complement(<1..910)
                     /locus_tag="PKX09_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HOU18373.1"
                     /translation="MAKYLTLREKIAYGFGDLASVLYWQTFMLYFTYFYTDVFLIPAG
                     VAATMFLVSRVWDAVNDPLMGIIADRTNTRWGKFRPYLLWFCVPLAVAGVLTFSVPNF
                     GMTGKIVYAYVTFILIMMLYTAINIPYTALLGVISPDSDERTSVSSIKFLFAFTAGVI
                     VSATLLPMVKAFGKGNEARGWQLSFVIYGIAAIIFFLIAFKGTRERVQPPKSQKSSIK
                     KDLYELVTNSPWLILLFTTITFVLFVAVKSSVTVHYFKYIVGTREISLPFFQNKTWDF
                     ETLTSVFNTVGQISSFIGVLLVSWFAK"
     gene            complement(1013..>1560)
                     /locus_tag="PKX09_10930"
     CDS             complement(1013..>1560)
                     /locus_tag="PKX09_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOU18374.1"
                     /translation="YIELGEISTYIPGYKENAGIFCHNNPWIMIAETKLGHGEQAFEY
                     WRKICPSFLEEISDLHRTEPYVYAQMIAGKDAYKPGEAKNSWLTGTAAWNFLAISQYI
                     LGIRPQFDGLLIDPCIPSDWKSYTVRRLFRGKNLNIEVRNPAGVQKGVRRITLNGEAL
                     SNNLIPCSKLKNTNEVIVDMG"
BASE COUNT          496 a          362 c          287 g          415 t
ORIGIN      
        1 ttttggcaaa ccagctaact aacaaaaccc caataaatga cgaaatctga ccaacggtat
       61 taaaaaccga agtaagtgtt tcgaaatccc aggttttatt ttggaaaaac ggcaaggaaa
      121 tctcacgcgt cccgacgata tatttaaaat aatgaaccgt cacactactt ttgacagcaa
      181 cgaagagaac aaaagttatg gttgtaaaaa gcaaaatcaa ccagggcgaa tttgtcacta
      241 attcatataa atcctttttt attgaggatt tttgcgactt gggtggctga acacgctcgc
      301 gcgtgccttt gaaagcaatt aggaaaaaaa taatagccgc aattccataa atcacaaacg
      361 ataactgcca accacgcgct tcgttgcctt taccgaaggc tttcaccatc ggcagaagcg
      421 tcgctgacac aataactccg gcggtaaacg caaacaaaaa tttgatagat gaaacggatg
      481 tgcgttcgtc cgaatccggt gaaattactc ccagcaatgc ggtgtatgga atattgatcg
      541 ccgtataaag catcataatc aggataaaag tcacataagc ataaacgatt ttaccggtca
      601 tcccaaagtt aggcacgctg aatgtcagaa caccagccac cgcaagcggt acacaaaacc
      661 acaataaata tggtctgaat ttcccccagc gggtgttggt gcgatcagct ataattccca
      721 tcaacggatc gttaacggca tcccagaccc gactgacaag aaacatcgta gccgctactc
      781 cggctggaat caagaaaaca tccgtgtaaa aataagtgaa atatagcata aaagtctgcc
      841 agtaaagcac cgacgccaga tcaccaaaac catatgcgat tttttcccgt aaggtaagat
      901 acttagccat tgtcctctcc tttttttaaa attttaatcc gtaatatctt aatttcactc
      961 cattataaag agtctattat ccccctaaac agacagtttt atctttaaat ttttaaccca
     1021 tatctacgat tacttcattg gtatttttca gcttagaaca aggaattaga ttattactca
     1081 atgcttcacc gtttaaagtt atccgccgca cacctttctg cactccggca ggatttctta
     1141 cttcaatatt taaatttttc ccccggaata gacggcgcac cgtatagctt ttccagtcgg
     1201 atggtatgca gggatcaatc agcaaaccgt caaactgtgg tcgaattccc agaatatatt
     1261 gcgagatcgc cagaaaattc catgccgccg tgccggttag ccaggaattc ttagcttcgc
     1321 ccggtttata ggcatcctta ccggcgatca tctgagcata gacatacggc tcagtacgat
     1381 gtaaatcgct gatctcctcg agaaacgatg gacagatttt gcgccaatat tcaaatgctt
     1441 gctcaccgtg acctaattta gtttcagcaa tcattatcca gggattgtta tggcaaaata
     1501 ttccagcgtt ttccttataa ccaggaatgt aagtcgaaat ctcacctaat tcgatgtaat
//
LOCUS       DAOSMP010000193         1549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_80884_length_1549_cov_10.124498, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000193 DAOSMP010000000
VERSION     DAOSMP010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80884_length_1549_cov_10.124498"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PKX09_10935"
     CDS             <1..640
                     /locus_tag="PKX09_10935"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOU18375.1"
                     /translation="RVRYKYHFYLPELFDWIAEEKPNGSLLEVGCSMGTDLLQLARRG
                     MRVTGIDLTEEGINLARKRFELYQMPAELKVDDAENLSFPDNTFDVVYSFGVLHHTPD
                     TQKAIDEVWRVLVPGGLAVIMLYNRKSLNYYIHRLINQPFDGNRKDRCPIERTYTKAQ
                     IFAMFKNFKVVKIETEYLLTTGYGIVWDLIPKAVHRALGHLWGWHLVIKAYK"
     gene            640..>1549
                     /locus_tag="PKX09_10940"
     CDS             640..>1549
                     /locus_tag="PKX09_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18376.1"
                     /translation="MKKVLFISYYWPPAGGATTTRILKFYKYLPEFGWEPIVLTVENG
                     DFPFIDSQTLKEVRPDTKIYKAKNISLHRLFAKMAKNSKEMFVPFAFTEKNNTGLKGK
                     LSRWVKYNAIPDTRFLWKKFAIKKGLQIIRENDIKLIFSSSPPQTNHCIATELSRKTG
                     IPCVGDMRDPWTDVYWLQTNSQRWKWIHNLDQKIEKRTLNSMAAITTIGPSLVNLFQK
                     KVNKPVYLIHNGFEPLDIDFAPRKNAKWTITYAGSISIEQDIQAFCKAIEVLIQNESI
                     KQNITIQFIGNFPEFVKQKLRQYSVFE"
BASE COUNT          494 a          305 c          311 g          439 t
ORIGIN      
        1 acgtgttcga tataaatatc acttctatct acctgaatta ttcgattgga tcgctgaaga
       61 gaaaccaaat ggttcactcc tcgaggtcgg atgcagtatg ggaacggatt tattgcaact
      121 tgccaggcgc ggaatgcgag taaccgggat tgacttgacg gaagaaggaa ttaatttagc
      181 ccgtaaacgc tttgaactat atcagatgcc agctgagctg aaagtagatg atgcggagaa
      241 tctctccttt ccggataata ccttcgatgt tgtctattct ttcggggttt tacatcatac
      301 tcccgatacc caaaaagcca ttgatgaggt ctggcgggta ttagttcccg gtggtttggc
      361 ggtgattatg ctgtataatc gtaaatcgct caattattac attcatcgtc tgattaatca
      421 gccttttgac ggtaatcgta aagatcgctg tccgattgaa agaacctata ccaaggctca
      481 aatattcgca atgtttaaaa attttaaagt tgtgaaaatc gagactgaat atttattaac
      541 caccggatat ggaattgttt gggatttgat tcccaaagca gtccatcgcg cacttggcca
      601 tctctggggc tggcatctgg taataaaagc atataaataa tgaaaaaggt tctgttcatt
      661 tcttattact ggccaccagc aggaggcgcg accaccacaa gaatcctcaa gttttacaag
      721 tatctacccg aattcggctg ggaaccgatc gttttgactg ttgagaacgg cgattttcct
      781 tttattgact ctcaaacatt gaaagaagtc agaccagata caaaaatcta taaggctaag
      841 aatatctcgt tacatagact atttgccaaa atggcgaaaa attccaaaga aatgttcgtt
      901 ccatttgctt ttacagagaa aaacaataca ggcctaaaag gaaagctatc acgctgggta
      961 aaatacaacg cgattcccga tacgcggttc ttatggaaaa aattcgcaat taaaaaaggt
     1021 ttacagatca ttagagaaaa tgatattaag ctaatctttt cttcatcgcc acctcagacg
     1081 aatcactgca ttgctacgga attatcacga aaaacaggta tcccgtgcgt cggcgatatg
     1141 cgagacccgt ggacggatgt ctattggttg cagaccaatt cccaacgctg gaaatggatt
     1201 cataatctgg atcaaaagat tgaaaaacgg actctcaatt caatggccgc tattaccaca
     1261 atcggtccat cgttagttaa tttatttcaa aagaaagtta ataaaccggt ttacctgatt
     1321 cataatggtt ttgaaccact tgatattgac tttgccccgc gaaaaaatgc aaaatggact
     1381 atcacttatg ccggaagcat cagtattgaa caggatattc aggcattttg taaagccatt
     1441 gaagtattga ttcaaaatga aagtatcaaa caaaatatta caattcagtt tattggcaat
     1501 ttccccgaat tcgtaaaaca gaaactgcgg caatattccg ttttcgaaa
//
LOCUS       DAOSMP010000194         1515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_83462_length_1515_cov_13.674658, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000194 DAOSMP010000000
VERSION     DAOSMP010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83462_length_1515_cov_13.674658"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..1315
                     /locus_tag="PKX09_10945"
     CDS             386..1315
                     /locus_tag="PKX09_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18377.1"
                     /translation="MRQVVDFGFAWNWEYDWDFANLLEADCRSEGLNFLQVTPEKIES
                     VLTEIGQGGIYLQALWDRAADTDSRFIELINWAKNNNIYQINPHEKAVRAWNKATMHL
                     EFITAGVNTPYTIILEPYNVRPVLPEIDLSVLGGRFIIKPAHGGGGLGVILDATSLER
                     VLTARRENPDDFYLLQAVIEPLQVNGRLAWFRVIYGFGKAYFCWWDVKTHIYTPFSNN
                     DKESLPMPLLNEIIEKIAGVTGLDVFSSEIALTAEGKFVVVDYINDPVDLRLQSRAAD
                     GVPDEIVRAISSDCVATVKKAARVNVTYEKLEN"
BASE COUNT          455 a          262 c          335 g          463 t
ORIGIN      
        1 tccgacattt cactctggtt ctcgactcat tgtcgttaaa acacttactt ttttctgcta
       61 aaaattccgc tcaaataatc ttgtcctctg taatgggtat ataattaatt tttttataaa
      121 aatcacgatg gatgataccg ttaagaaacc aatagcggta aaaaattggg ctcataaagc
      181 tggaagagtt agttttctat tccttggttc attaccggat agtaaaattt tatcgagtaa
      241 tacttttgac ggctcgttta aaaaccttaa cttttgttaa atgattagtg ataataatgg
      301 ttttatttaa aatattatcc tttgtgcaac ttgatgaata atcctaatat cgtcctgcat
      361 ttgataaccg agaagtgaat tttagatgcg gcaggtagtt gatttcggct ttgcctggaa
      421 ctgggaatat gattgggatt ttgccaattt attagaagct gattgccggt cggaaggatt
      481 aaattttctt caagtgactc cagaaaagat cgaatcggta ctgactgaaa tagggcaagg
      541 tggaatttat ttacaagcat tatgggatag ggcggctgac accgatagcc gatttataga
      601 actgataaac tgggctaaga ataacaatat ttaccagatc aatcctcacg aaaaagccgt
      661 cagagcctgg aacaaagcga caatgcacct ggaatttatc acagcaggag tcaatacacc
      721 ttatacgatt attttggaac cttataatgt ccggccagta ttgcccgaga ttgacttgag
      781 tgtcctaggc ggtcgtttta taatcaaacc ggcgcacggc ggcggcggct tgggtgtaat
      841 tctggacgcg accagtctgg agcgcgtatt gactgctcgt cgagaaaatc cggatgattt
      901 ttatctttta caggctgtaa tcgaaccgtt acaggtcaac gggcggctgg cttggtttcg
      961 ggtcatttat ggcttcggaa aggcttattt ttgctggtgg gatgtcaaga cgcatattta
     1021 tacccctttt tcgaataatg ataaagagag cctgccaatg ccattactaa acgaaataat
     1081 cgagaaaatt gccggtgtga cgggactgga tgttttttct tctgaaattg ctctgacggc
     1141 agaaggtaaa tttgtggtag tggattatat taacgatccg gttgatttgc gtttgcaatc
     1201 cagagcggca gatggcgtcc ccgatgaaat cgtccgagcg atttcatcgg attgcgttgc
     1261 cactgtaaaa aaagctgctc gggtaaatgt aacttatgaa aaattggaaa attagagaat
     1321 tggataagtt gatatttaat tcaatccaga aaattctaac tgcttattta tgggttacca
     1381 atttttaatt acgagaaata ttttggcttt attatgacta tcatctaaat taacgtaatt
     1441 aaaaaaagga gcctttaaag ccaaaccgtg agttgggaaa ggcaaaagag aaaccagaaa
     1501 tccacccaga gcggt
//
LOCUS       DAOSMP010000195         1506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_84190_length_1506_cov_7.847002, whole genome shotgun sequence.
ACCESSION   DAOSMP010000195 DAOSMP010000000
VERSION     DAOSMP010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84190_length_1506_cov_7.847002"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1506
                     /locus_tag="PKX09_10950"
     CDS             <1..>1506
                     /locus_tag="PKX09_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOU18378.1"
                     /translation="ISVGSTDYADNKSDFSNYGQATVDIGAPGTDIASCYPYNSYVYM
                     SGTSMAAPHVTGVIALAKSVFPEDDAHDLIARTLATADPVTNLRGLWNSEGRLNAHLA
                     VDPTVIGGFKRSVPTAAYIQKNFTDDYGVATVGFVNGKDIDVSVTSATINGDQASCFS
                     LPTMTFPITVKAGGAFGIPVMFSPNAGPTASYTATLTITAGLDGVIIPLVGIEQGYAE
                     MAIEPEFTDLGVVHYGDTLTSTFTITNTGEADLNWHLFQYLISNNVEPEYNLTALNNF
                     KPQPALTNKTPKNKTEVIQKDFEKIAPVIAELNRPKITLDFNQEVENGYNENILWADS
                     LNDSTATMEQWTLMAFGSDEGANETWRLVDISDGGPQDFVFLAGDFGYGYKNNTIAVA
                     ATPLFDFTPTADGKGAAYLAFDYAAQIEQGYDYFYVNVIVNGERWGTILQTEWNLWTG
                     GTTYRAYADISELVGQKNVEFWFILNTDFSNAYGFGALFDNVEIGVMDLPY"
BASE COUNT          403 a          329 c          350 g          424 t
ORIGIN      
        1 ttatcagtgt cggttccact gattatgctg acaataaatc ggatttttcg aattacggtc
       61 aagccacagt tgacatcggc gcaccaggta ctgatattgc gtcttgttat ccttataata
      121 gttatgtgta tatgagcggc acatctatgg cggcgccgca tgtcactggc gtaatcgctc
      181 tagcaaagag cgttttcccg gaagatgatg ctcatgatct tatcgctcgc acgctggcta
      241 ctgctgatcc ggtgaccaat cttagaggtt tatggaattc cgaaggacga ctcaatgctc
      301 acctggcggt agatcctact gtaataggtg ggtttaagag aagtgttccc acagcagcat
      361 atatccagaa aaatttcact gatgattatg gcgttgcgac ggttggcttt gtgaacggca
      421 aggacattga tgtttcggta acatcggcaa ctatcaacgg ggatcaagcc agttgcttca
      481 gtttaccgac aatgactttc ccgattacag tgaaagctgg cggagctttt ggcatcccgg
      541 ttatgtttag tccgaatgct ggaccgaccg cctcatatac cgctactttg actattacgg
      601 ccgggttgga cggcgttatc attccattag ttggaatcga acaaggttat gccgaaatgg
      661 caatcgaacc ggagtttacc gatctgggag tagtccatta tggtgatact ctcacttcta
      721 ctttcactat cacgaatact ggtgaagctg acctgaactg gcatctcttc cagtatttaa
      781 tcagcaataa cgtagaaccg gaatataatc taacggctct taataatttc aaaccccagc
      841 cagcgctgac taacaagacg cctaaaaaca agaccgaagt cattcagaaa gattttgaga
      901 aaatcgctcc ggtaatagct gaattgaatc gtcccaaaat cacgctcgat ttcaatcaag
      961 aagtcgaaaa tggctacaac gaaaatatcc tctgggcgga tagtttgaat gactctaccg
     1021 ccacaatgga acaatggact ttgatggcat tcggctctga cgagggcgct aatgaaactt
     1081 ggcggctggt agatatatcg gatggtgggc ctcaagactt tgtatttctt gctggtgatt
     1141 ttggttatgg ctataaaaac aacacgattg ccgttgccgc taccccgctg ttcgatttta
     1201 ctccaactgc tgatggtaaa ggagcggctt atctggcttt cgattacgcc gctcaaatag
     1261 aacaaggcta tgactatttc tatgtcaacg tcatcgtaaa tggtgaacgc tggggcacca
     1321 ttctccaaac cgaatggaac ctatggactg gtggcactac ctatcgagcc tatgccgata
     1381 tttccgaact agttggacag aagaatgttg aattctggtt tattctcaat actgacttta
     1441 gtaatgcata tggtttcggt gctctattcg ataatgttga aatcggagta atggatttgc
     1501 catatt
//
LOCUS       DAOSMP010000196         1483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_86015_length_1483_cov_8.267507, whole genome shotgun sequence.
ACCESSION   DAOSMP010000196 DAOSMP010000000
VERSION     DAOSMP010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86015_length_1483_cov_8.267507"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1440)
                     /locus_tag="PKX09_10955"
     CDS             complement(<1..1440)
                     /locus_tag="PKX09_10955"
                     /inference="COORDINATES: protein motif:HMM:NF014149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU18379.1"
                     /translation="MRINGNVTSSTMPVQNAVVTFENAFDPSCRFSTLTDSAGFYRLE
                     VTTSAITPPYKIPTKFDLGQNYPNPFSATTVIPYSLKQDSRVFLTIYDLLGREVRKIE
                     AGVQPVGSYQVLWDGCNNHGQKVATGLYFYRLEAGGESRIRKLIFDGSGLHQSVTTMF
                     QSIQVPKFELAKSMNLSGQNFTVKLDNCDSTFPVIIPKQVENVYLANDTTINFTVSIV
                     PIATVYPDSLKQYIRGFGAANILPWRPDMTDAEIEKAFGTGDGQIGFSMLRLRLPSEE
                     TTASFQTQVATAKKAYDRGVLVFASPWSPPARMKSNNSTIDGYLLESHYQDYVDHLNS
                     FIDYMADNGVDLYAVSVQNEPDVDVSYESCDWSPEQMRKFMAENAGTIKTRVIAPESY
                     QFRREMSDPILNDSAACANLDIVGGHIYGGGLGAYPLAEQKGKEIWMTEHLTGEKSHS
                     NDWTWAFDVAKEMHSCMEAGFSAYVWWYIV"
BASE COUNT          430 a          349 c          290 g          414 t
ORIGIN      
        1 aacaatatac caccagacat aagcgctgaa tccagcctcc atacaactat gcatttcttt
       61 agccacgtca aatgcccagg tccaatcatt ggagtgactt ttttctccgg ttaaatgctc
      121 ggtcatccaa atttccttgc ctttttgttc tgccagcgga taagccccta aaccaccgcc
      181 gtaaatatgc ccgccgacaa tgtccaggtt ggcgcacgcc gctgagtcat tcaatatcgg
      241 atcagacatc tctctcctga actggtagga ttccggcgca atgactctgg ttttaatagt
      301 tccagcattt tccgccataa atttccgcat ctgttccgga ctccagtcac aagattcgta
      361 agatacatca acatccggtt cgttctgaac agagaccgcg tataaatcaa caccattatc
      421 agccatataa tcaataaaag aatttaaatg atcgacataa tcctgataat gagattccag
      481 aaggtaaccg tcaatagtgc tgttattact tttcattcta gctggtggtg accagggtga
      541 agcaaaaacc aaaactcccc ggtcataagc ttttttcgcg gtcgcaactt gcgtttgaaa
      601 agacgcggtg gtttcttctg aaggcagtct caatcttaac atcgagaaac caatttgccc
      661 gtcgcctgtg ccaaaagcct tttcgatttc agcatcagtc atatccggac gccagggtaa
      721 aatattagcc gccccaaaac ctcgaatata ctgcttcaga ctatccggat aaacagtagc
      781 aattgggaca atggacacgg taaaattgat agtcgtgtca tttgcgagat acacattttc
      841 aacctgtttt ggtataatta caggaaaggt gctgtcacaa ttatcaagtt ttaccgtaaa
      901 attctgaccc gatagattca tactttttgc cagttcaaat ttcggcacct gaattgattg
      961 gaacattgtg gtcacggatt ggtgtaatcc acttccatcg aaaatcagtt tgcgaattct
     1021 ggattcgccg ccggcttcca gccggtaaaa atataatccc gtcgctactt tctgaccatg
     1081 attattacaa ccgtcccata gaacctggta actccctact ggctgaactc cagcctcaat
     1141 tttacgtact tctcgcccaa gtagatcata gatagtcaaa aaaactctgg aatcttgctt
     1201 cagagaataa ggaatcacgg tcgtagctga aaacggattg ggataattct gtcccagatc
     1261 aaatttggta gggattttat aaggtggtgt tatcgcgctc gttgtcactt ccaaccgata
     1321 aaaacccgct gaatcggtta aagtcgaaaa tcggcaggaa ggatcaaaag cattttcaaa
     1381 agttactact gcattttgaa ccggcatagt cgaactggtt acattaccgt tgatgcgcaa
     1441 tgcttgagga tataactgtg aaaataacat tatcgagttt aga
//
LOCUS       DAOSMP010000197         1482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_86110_length_1482_cov_15.159075, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000197 DAOSMP010000000
VERSION     DAOSMP010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86110_length_1482_cov_15.159075"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PKX09_10960"
     CDS             complement(<1..842)
                     /locus_tag="PKX09_10960"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOU18380.1"
                     /translation="MTIPISKRAQALKPSPTMSVTALASKMAAEGISIINFGVGEPDF
                     NTPENIKQAAHQALDANFTHYTVSAGILELRQAICDKLKRDNNLDYSPDEIIVAPGGK
                     AAIITILAAICDPDDEIIIPAPYWVSYPSQVEMLDGKPVFIATDESTNFKITADQLES
                     VIKNLSQPKALILNSPNNPTGAVYSPTELAAIGAVCLKHNLAVISDEIYEKLVYGSAR
                     HYSIVQVLPEIRSITIVINGFSKAYAMTGWRVGYAAGPREVIKRATAIQEHTASCVTS
                     ITQKA"
     gene            complement(859..>1482)
                     /locus_tag="PKX09_10965"
     CDS             complement(859..>1482)
                     /locus_tag="PKX09_10965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOU18381.1"
                     /translation="FKWNQNKITTIITTKSTYSCRWVINAAGAYARRIGEMLELDLPV
                     FPESHEGGITEPVSRFFAPMIVDVRPDSESSNYYFYQNSEGQVVFCITPEPKIAGTDC
                     DSTSNFLPLVTKRMLSLYPRLRHLRVRRTWRGLYPATPDGSPIVGFTHQFCNLFLAIG
                     MCGQGFMIGPGLGAIIAEILTDNTHKYDEILDQWNLYRNFTGQEILK"
BASE COUNT          384 a          308 c          384 g          406 t
ORIGIN      
        1 gctttttggg tgattgaagt aacgcaggag gcggtatgct cttgaatggc ggttgcccgc
       61 ttgatgacct cacgtggtcc ggcggcataa cctacgcgcc agccagtcat cgcataggct
      121 ttactgaagc cgtttatgac aattgtaatg gatcggattt caggcaaaac ctggacgatt
      181 gagtaatgcc gagcagagcc gtaaactaat ttttcgtaaa tctcgtcgga aatcactgcc
      241 aaattgtgtt ttagacaaac tgcgccgata gcggctaatt cggttggcga ataaacggcg
      301 ccggttggat tgttcggtga attaaggatt agtgctttcg gttgcgaaag gttcttaata
      361 accgattcca gctggtcggc ggtaattttg aaattggttg attcgtcggt ggcgatgaaa
      421 accggcttgc catccaacat ctcgacttgg gaaggataac taacccaata aggggcggga
      481 atgataattt catcgtcggg gtcacaaatt gcggcgagga tggtgattat ggcggctttt
      541 ccgccgggtg ctacgatgat ttcgtcgggt gaatagtcca gattattatc acgtttgagt
      601 ttatcacaaa ttgcctggcg taattccaga atgccggctg agacggtata atgagtgaag
      661 ttggcatcca gcgcctgatg agcggcttgt ttgatatttt ccggagtatt aaaatctggt
      721 tcgccgaccc caaagttgat tatggaaatg ccttcagccg ccatttttga agccaaagcg
      781 gttacagaca tagttggtga cggttttaag gcttgggctc gttttgaaat aggaatagtc
      841 atagaagctc ctcttatttt attttaaaat ttcctggcca gtaaaattac gatagagatt
      901 ccactggtcg agaatctcat catatttatg agtattgtcc gttagtattt cggctattat
      961 tgcaccgaga ccgggaccaa tcataaagcc ctgtccacac attccaatcg ccaaaaataa
     1021 attgcagaat tgatgagtga atccgacgat cggagaaccg tccggcgtgg caggataaag
     1081 tccacgccag gtgcggcgca cgcgcagatg cctcaggcgt gggtaaagac taagcatcct
     1141 tttggtaact aaaggtagaa agttcgaagt actgtcacag tcagttccgg cgattttagg
     1201 ttcgggtgtg atgcaaaaga ccacctggcc ttcgctattt tggtaaaaat aataattgct
     1261 tgattcgctg tccggtcgga catctacgat catcggtgcg aaaaagcggc taaccggttc
     1321 ggtaataccg ccttcgtggc tctccgggaa aaccggcaaa tcgagttcca gcatttcgcc
     1381 aatgcgacga gcataggctc cagcggcatt gattacccaa cgacatgaat aggtggattt
     1441 agtagtaata atggtcgtaa ttttattttg attccactta aa
//
LOCUS       DAOSMP010000198         1478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_86458_length_1478_cov_7.513703, whole genome shotgun sequence.
ACCESSION   DAOSMP010000198 DAOSMP010000000
VERSION     DAOSMP010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86458_length_1478_cov_7.513703"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="PKX09_10970"
     CDS             complement(<1..908)
                     /locus_tag="PKX09_10970"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOU18382.1"
                     /translation="MKKGRSTLILFGIFVVLFAAVYFFEIKGGEKRAKTSKLASQLFQ
                     TEKDNVKQLILSGDYGEITCEKNGAEWIITAPVLAQGDAQSIESTLDLILGASIERKT
                     ADSTDDLSWFGLLEPRGKVQVVTQSGKHTIVLIGDENPTGDLIFVKYPDDNAIYTTNK
                     SLWTYANKKLYDLRDKKIMHFNLDEVRKINITSRAKGKVSLEKVAGKWQIVDPVKLPA
                     NDSEVKSLLTRLSTGRAKEFIAEEPDDLKKYGLTNPSVMITLELGESLARTSFLVGDT
                     ATNDGGGFYAKEETRRPVFTVEKWTV"
     gene            complement(912..>1478)
                     /locus_tag="PKX09_10975"
     CDS             complement(912..>1478)
                     /locus_tag="PKX09_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18383.1"
                     /translation="RLFGAGPDIPLVTDYTDHPIVKDMKNVMTFFPMARTVRPLESDN
                     KENIIVEELIRTGQQSFAVANIEEVYRTGRVSRSPNDLQGPLSIACAATIENPSGQRE
                     GRLVVVGDSDFASNAYFDNQANGDLFMNMISWLIADEDLIAIRPKNPEISTVNMTPAQ
                     TKTVMWLTVIILPAIAFGVAIMVYVRRK"
BASE COUNT          382 a          355 c          306 g          435 t
ORIGIN      
        1 tccacagtcc atttttcaac cgtaaagacc ggtcgccggg tttcttcttt agcgtaaaaa
       61 ccgccaccat cattggttgc ggtatcacca acaagaaaac tggtacgcgc caggctttcg
      121 cccagttcaa gagtaatcat aacggatgga ttggtcaatc catatttttt aagatcatct
      181 ggttcttcgg caataaactc cttcgctcta ccggttgata ggcgagttag caaggatttg
      241 acttcgctat catttgccgg tagttttact gggtcaacga tttgccattt tcccgcgact
      301 ttttccaggc tgacttttcc ttttgcccgg gaagtgatat tgattttgcg cacttcatct
      361 aaattgaaat gcattatctt tttatcacgc aagtcgtata gttttttatt agcataagtc
      421 cagagtgatt tattggtagt ataaatcgca ttgtcatccg gatatttgac aaaaatcaag
      481 tcgccggttg gattctcgtc accgatcaat actatggtat gtttaccact ttgagtaacg
      541 acctgcacct ttccgcgcgg ttctaataaa ccaaaccagc ttaaatcgtc ggttgagtcg
      601 gcagtctttc gttcgatact tgcccctagg attaaatcga gggtggattc gatactctgg
      661 gcgtcgccct gggctaacac cggtgcggtg ataatccact ccgctccgtt tttctcgcaa
      721 gtaatttctc cataatctcc agagagaatc aattgtttga cgttgtcctt ttcggtctgg
      781 aagagttggc tggctaattt gcttgtcttt gcccgcttct cgccgccttt aatttcaaaa
      841 aaataaacag ccgcaaacaa gacaacaaaa atcccgaata aaatcagagt tgatcggcct
      901 tttttcatgg tttatttcct cctgacataa accataatcg ccaccccgaa agcaattgct
      961 ggcaaaatta taacggtcag ccacataacc gttttggtct gggcgggagt catattaacc
     1021 gtactaattt ccggattttt aggacgaatc gcgatcaggt cttcatcggc aataagccag
     1081 ctgatcatat tcataaataa atcaccatta gcttgattat cgaaataggc attcgaggca
     1141 aaatcggaat caccgaccac taccagccga ccttcacgct gaccggaagg gttctcaatt
     1201 gtagccgcac aggcgattga aagtggaccc tgcagatcat tcgggcttct ggaaacgcga
     1261 ccagtgcggt aaacctcctc gatattggca accgcaaaac tctgctgacc cgttcggatt
     1321 aattcctcga ctattatatt ctctttgtta tcagactcca gcggtctaac tgttctagcc
     1381 attgggaaga aggtcattac atttttcata tcctttacaa tcggatgatc ggtatagtca
     1441 gtaacgagcg gaatatctgg cccggcaccg aaaaggcg
//
LOCUS       DAOSMP010000199         1470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_87141_length_1470_cov_17.792226, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000199 DAOSMP010000000
VERSION     DAOSMP010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87141_length_1470_cov_17.792226"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /locus_tag="PKX09_10980"
     CDS             complement(<1..1397)
                     /locus_tag="PKX09_10980"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HOU18384.1"
                     /translation="MKFHQLTRNLFLVAIIMVGLTSVSGATDLNIADLRQNDESGTPV
                     YLDQVVTTTGIVTVANEFGATGPVYIQNEGAGIALYGAGYVAARLMPGYVVNVTSKVG
                     FYSGLTELLWIDTVTVVNILDKTEIPEPTIVSISEILNQQWDGYEALEGSLLKLENVS
                     FQMIGNFAGNTNYTITDNIGYSMDIRIGNDVDLVGSPIPTVPVTIVGVLSQFDSSSPF
                     SEGYQLLPRSSSDITGGQGGGTTVLSYSFDKTTSDPLVSTWATGSGQINLSDNTSIIK
                     EGIAALEVGWQIVHEYEWGGNGTINYAKNAPVGEYLDISGADYLTLWVYNAVPASQSE
                     RTLFEFHLLEAGGDTVYDGTCNSAEHWLYRISGVFDQRPGWNQIRMPLIDRGAEHPND
                     QGFSAPGWNGVANNGILDKDRIVGWQIIIQTNNTSMGDIVTGTTLFDQLQLETGGGSN
                     LTSQDVTVTFSVDMS"
BASE COUNT          412 a          364 c          284 g          410 t
ORIGIN      
        1 ctcgacatat ccactgaaaa agtaactgta acatcttgag aggtaagatt actaccaccc
       61 ccggtttcta actgcaactg gtcaaaaaga gtcgtcccag taacaatatc acccatagaa
      121 gtgttgttag tttgaatgat tatttgccag ccaacgattc tatctttatc caaaatacca
      181 ttgttagcta ctccattcca gcccggcgca ctaaaaccct gatcattagg atgttcagca
      241 ccacggtcaa ttagcggcat ccggatctgg ttccagccgg gtctttgatc aaatacacct
      301 gatatccggt atagccaatg ctccgcgcta ttgcaagtcc catcatagac ggtatcgcca
      361 ccggcttcca gaagatgaaa ttcgaataaa gttctctcgc tctgtgaagc tggcaccgcg
      421 ttatataccc ataatgtcag ataatcagcg cctgaaatat caagatattc accaaccgga
      481 gcgttttttg cataattaat tgtgccattt ccgccccatt cgtattcatg gacaatctgc
      541 cacccaactt caagcgccgc tattccttct ttaattatgg aggtgttatc tgataagtta
      601 atttgacctg atccggttgc ccaggttgaa accagtggat cactggtggt tttgtcaaag
      661 gaataactta aaacggtcgt gccaccacct tgtccgcctg taatatcact ggatgaccgc
      721 ggcaatagct gatagccttc actaaaaggt gaactagaat caaattggct taaaacaccc
      781 acaatcgtta ctgggacagt aggaatcggt gatcctacta gatccacatc gttgccaatc
      841 cggatgtcca tagaatagcc aatattgtca gtgattgtat agttcgtatt tccggcaaaa
      901 tttcctatca tctgaaacga tacattttcc agcttcagca gtgatccttc tagcgcttca
      961 tagccgtccc attgttgatt tagaatctca ctaattgaaa cgatggtcgg ttcgggaatt
     1021 tcggttttat ccagaatatt tactacagta accgtatcaa tccatagcag ttcagtaagt
     1081 ccgctataga atccgacttt gctcgttaca ttcacgacat aaccgggcat caatctggcg
     1141 gctacgtacc ccgctccgta tagagcaata ccggcgccct cattctggat atataccggt
     1201 cccgtagcgc caaattcatt agctaccgta acgattcccg tagttgtgac aacctggtca
     1261 agatagaccg gcgtgccaga ctcatcgttc tggcggaggt cggcaatgtt tagatcggtt
     1321 gctccactaa cactagttaa gccaaccata ataattgcca ccagaaaaag atttcgagtt
     1381 aattgatgaa acttcatttg gtctctctcc tcatataaaa ttaaatgtta ataaactgcg
     1441 gttggctaat gactgccgca aatatccaaa
//
LOCUS       DAOSMP010000200         1466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_87497_length_1466_cov_12.990078, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000200 DAOSMP010000000
VERSION     DAOSMP010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87497_length_1466_cov_12.990078"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PKX09_10985"
     CDS             complement(<1..913)
                     /locus_tag="PKX09_10985"
                     /inference="COORDINATES: protein motif:HMM:NF015659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase-associated domain-containing
                     protein"
                     /protein_id="HOU18385.1"
                     /translation="MKNYLNNLKTGLIIFGLIASMITSIPKGANEMQTLTVHYHRYDG
                     AYQNWTLWTWSENFSTEIKATGQDDYGLIFNLDLSIYPNPKTIGLLPKYGSWERKDDP
                     DRYWKPNSHHEVWLLQGDETVYNSRPDISPKIRRAFLDSPTTVTLVFSNAITKEMSNT
                     LTATIKLKNGQEIKPGKISLFPEHSHSSEIVRLMDLPPIEIDHLPATVSMSNFADCPV
                     WLRYIRDDEKFITEEPLGAFYSPDSTRFAIFAPGAKTVVLHIYDQPQGGKPTKYKLTK
                     IQSSLWSVTIKGDLKGKYYVYSVAGPDP"
     gene            1139..>1466
                     /locus_tag="PKX09_10990"
     CDS             1139..>1466
                     /locus_tag="PKX09_10990"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU18386.1"
                     /translation="MEKQIRKLTILFLCFLIWAVGLFSDAPEPVPKTSDAAQNVVRIG
                     VLKLPAVLVKGGTFEMGSDDESSPEDTRPAHTVTLTDFYISVTEVTVGQYKEYCRQTG
                     KSMPEQE"
BASE COUNT          417 a          265 c          340 g          444 t
ORIGIN      
        1 ccggatcggg accggcgacg ctataaacat aatatttccc tttcagatca cctttaattg
       61 ttacagacca gaggctgctc tggattttgg tcaatttata tttggtaggc ttgccacctt
      121 gcggttgatc ataaatatgt aataccaccg tcttagctcc gggagcaaaa attgcaaacc
      181 tggtagaatc aggtgaataa aaagcaccga gcggctcctc ggttatgaac ttttcatcat
      241 ctctaatata cctgagccag acaggacaat ccgcaaaatt ggacattgaa acggttgccg
      301 gcagatggtc aatttcaatc gggggcaaat ccataagccg cacaatttct gaggaatggg
      361 aatgttccgg gaataggcta attttaccgg gtttgatttc ttgaccgttt tttagtttga
      421 tggtagccgt gagggtattt gacatttctt tggtaattgc attggagaag acgagcgtaa
      481 ctgtggtagg actatcgaga aaagcccggc ggattttggg gcttatatca ggacgagagt
      541 tatagactgt ttcatcaccc tgtaagagcc agacttcgtg atgggaattc ggtttccagt
      601 aacgatctgg atcatctttt ctttcccaac tgccatactt tggtaaaaga ccgatagttt
      661 tgggattggg ataaatactc agatcgaggt taaaaatcag accgtagtca tcctgtccgg
      721 tcgctttgat ctcagtgctg aagttttcag accaggtcca gagcgtccaa ttctggtaag
      781 ctccatcata gcggtgataa tgaacagtga gggtttgcat ttcgttagcg cctttcggta
      841 tagaggtgat catactggca atcaaaccaa atatgatcaa tccggttttt aaattgttta
      901 ggtagttctt cattgaacga ttccttttta ggaatgatta acatatccaa tattaacaaa
      961 ctttgttttt aaataacata aaaaattgcc atttctaaat aattctgacg atatgtggaa
     1021 ttttatcgtg tttttaaagc tcatttaagt cgtcaaaagc gagtacgaga taaaaagtcc
     1081 ttgatattcc gaagttcttt ttatagttta attatagatt taagggatgt aataggaaat
     1141 ggaaaagcaa atacgaaagt taacaattct ttttttgtgc tttctaattt gggctgttgg
     1201 gctttttagt gatgctcctg aaccggttcc aaagacgagt gacgcggccc agaatgtggt
     1261 aagaattgga gttttaaagc taccggcggt gctggtcaag ggtgggactt ttgaaatggg
     1321 ctctgatgac gaaagtagtc ccgaggatac aagaccggct cataccgtca cactgactga
     1381 tttttacatt agtgtaacgg aagtgactgt cggtcaatac aaagagtatt gtcgtcaaac
     1441 aggtaaatct atgccggaac aggaag
//
LOCUS       DAOSMP010000201         1465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_87588_length_1465_cov_14.041844, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000201 DAOSMP010000000
VERSION     DAOSMP010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87588_length_1465_cov_14.041844"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PKX09_10995"
     CDS             complement(<1..913)
                     /locus_tag="PKX09_10995"
                     /inference="COORDINATES: protein motif:HMM:NF015659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase-associated domain-containing
                     protein"
                     /protein_id="HOU18387.1"
                     /translation="MKNYLNNLKTGLIIFGLIASMITSIPKGANEMQTLTVHYHRYDG
                     AYQNWTLWTWSENFSTEIKATGQDDYGLIFNLDLSIYPNPKTIGLLPKYGSWERKDDP
                     DRYWKPNSHHEVWLLQGDETVYNSRPDISPKIRRAFLDSPTTITLVFSNAITKEMSTS
                     LAPTIKLKDGQTIQPGRICLIPENSHSSEIVRLLDLPAIEIDNLPATVSIPNFADCPV
                     WLRYIRDDEKFITQEPLGAFYSPDSTKFAIFAPGATAVVLHIYDQPQGGKPTKYKLTK
                     IQNSLWSVTVKGDLKGKYYVYSVAGPDP"
     gene            1138..>1465
                     /locus_tag="PKX09_11000"
     CDS             1138..>1465
                     /locus_tag="PKX09_11000"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU18388.1"
                     /translation="MKKQTRKLTILFLCFLIWAVGLFSDAPEPVPKTSDVAQNVVRIG
                     VLKLPAVLVKGGTFEMGSDDESSPEDTRPAHTVTLTDFYISVTEVTVGQYKEYCRQTG
                     KSMPEQE"
BASE COUNT          417 a          266 c          345 g          437 t
ORIGIN      
        1 ccggatcggg accggcgacg ctataaacat aatatttccc tttcagatca cctttcaccg
       61 ttacagacca gaggctgttc tgaatcttgg tcaatttata tttggtaggc ttgccacctt
      121 gcggttgatc ataaatatgg agcactactg ccgtagcacc gggcgcgaaa attgcaaact
      181 tggtagaatc gggtgaataa aaagcgccaa gtggctcctg ggttatgaac ttttcatcat
      241 ctcgaatata cctgagccag acaggacaat ccgcgaaatt gggtattgaa acggttgccg
      301 gcaagttatc aatttcaatc gcaggcaaat ccaataatct tactatttcc gaggaatggg
      361 agttttcggg aatcagacaa attcggccag gctggattgt ttgaccatct ttcaatttga
      421 tggttggggc gagggaagtt gacatttctt tggtaattgc attggagaag acgagcgtaa
      481 tggtggtagg actatcgaga aaagcccggc ggattttggg gcttatatca ggacgagagt
      541 tatagactgt ttcatcaccc tgtaagagcc agacttcgtg atgggaattc ggtttccagt
      601 aacgatctgg atcatctttt ctttcccaac tgccatactt tggtaaaaga ccgatagttt
      661 tgggattggg ataaatactc agatcgaggt taaaaatcag accgtagtca tcctgtccgg
      721 tcgctttgat ctcagtgctg aagttttcag accaggtcca gagcgtccaa ttctggtaag
      781 ctccatcata gcggtgataa tgaacagtga gggtttgcat ttcgttagcg cctttcggta
      841 tagaggtgat catactggca atcaaaccaa atatgatcaa tccggttttt aaattgttta
      901 ggtagttctt cattgaacga ttccttttta ggaatgatta acatatccaa tattaacaaa
      961 ctttgttttt aaataacata aaaaattgcc atttctaaat aattctgacg atatgtggaa
     1021 ttttatcgtg tttttaaagc tcatttaagt cgtcaaaagc gagtacgaga taaaaagtcc
     1081 ttgatattcc gaagttcttt ttatagttta attatagatt tagggatgta ataggaaatg
     1141 aaaaagcaaa cgcgaaagtt aacaattctt tttctgtgct ttctaatttg ggctgttggg
     1201 cttttcagtg atgctcctga accggttcca aagacgagtg acgtggctca gaatgtggta
     1261 agaattggag ttttaaagct accagcggtg ctggtcaagg gtgggacttt cgaaatgggc
     1321 tctgatgatg aaagtagtcc cgaggataca agaccggctc ataccgtcac gctgaccgat
     1381 ttttatatta gtgtaacaga agtgactgtt ggtcaataca aagagtattg tcgtcaaaca
     1441 ggtaaatcta tgccggaaca ggaag
//
LOCUS       DAOSMP010000202         1439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_89918_length_1439_cov_15.894509, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000202 DAOSMP010000000
VERSION     DAOSMP010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89918_length_1439_cov_15.894509"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..1124
                     /locus_tag="PKX09_11005"
     CDS             78..1124
                     /locus_tag="PKX09_11005"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOU18389.1"
                     /translation="MIIEKAALFDFLTRCQKENLGDFKQVIAPRRSDKSGDVLFFDNW
                     REGAEFVIDSYRTVDPLRMLFYMARERILPAMTSNEKWLIVGAKACDLQALEVLDKAL
                     INADFVDPLYENRRKNVTIISADCNEIAPTCHCNLVNGRPYATKSFDLNLTRLDTKYY
                     HLEVGSGRGQELLDLMQKNAKISRNLEDDLNTRDENRKAIEKRLILQNKQYERSQQYE
                     KLKAADLTKWVDEAFTCCGCGGCTNICPTCYCLILNDETSSEIFTKDRSTDTCQLHGY
                     ARVAGGATPRPKMFERFRNRYLCKFSYVQSNFGMIGCTGCGRCIEVCAGKIEFREVLQ
                     RIMSLGIVTEASNV"
     gene            1117..>1439
                     /locus_tag="PKX09_11010"
     CDS             1117..>1439
                     /locus_tag="PKX09_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOU18390.1"
                     /translation="MSKNPYETTAAVIYEIIEESPTIRTLRLRPEKPMPFRTGQFMEV
                     TVPGIGEGPFTPSSSHYIADTLDFTIMKTGFVTEYIHRTKVGDRLGLRGPYGKGYPLT
                     EFKGK"
BASE COUNT          401 a          282 c          361 g          395 t
ORIGIN      
        1 aaatggcagg gggtaaagcc tgaatcagtt tttaaaaaat actttatgga ttgagatttt
       61 cagatggagg tctattaatg ataattgaaa aagccgcttt attcgacttc ttaacgcgtt
      121 gtcagaagga aaatctgggt gatttcaaac aagtgattgc accgcgtcgt tccgataaat
      181 ccggtgatgt tctatttttc gataattggc gggaaggggc agaatttgtg attgacagtt
      241 atcggacggt tgatcccttg cgaatgcttt tctatatggc gcgggagcga attttaccgg
      301 ctatgacgag taatgagaaa tggttgattg tcggcgccaa agcctgtgac ctgcaagctc
      361 tggaagtatt ggataaagcc ctaataaatg ccgattttgt cgatccgtta tatgagaacc
      421 ggcgtaaaaa tgtaacaatc attagtgccg attgtaatga aatcgcgccg acctgccact
      481 gcaatttagt caatggtcga ccctatgcta ctaaaagttt tgacctaaat ctcaccagac
      541 tcgatacgaa gtattatcac ctggaagtcg gttcagggag gggacaggaa ttactcgatt
      601 taatgcagaa aaacgcgaaa attagccgga atttagaaga tgatttaaat acgcgcgacg
      661 agaaccgtaa agccatcgaa aaacgtttga tattacaaaa taagcagtat gaacgtagtc
      721 agcagtatga gaagcttaaa gccgccgatt taactaaatg ggttgacgaa gcttttacct
      781 gttgtggctg tggtggctgt accaatattt gtccaacctg ttactgcctg attctaaacg
      841 acgaaaccag cagtgagatc tttaccaaag atcgcagtac cgatacctgt cagttacacg
      901 gttatgcgcg ggtggcaggg ggcgcgacgc ctcgaccgaa gatgttcgag cgttttcgca
      961 atcgctattt atgcaaattt agttatgtgc aaagcaactt cgggatgatt ggttgcaccg
     1021 gttgtggacg ttgcattgaa gtttgcgccg gaaaaatcga atttcgtgag gtattacaac
     1081 ggatcatgtc cctcggaatt gtcacggagg cgtccaatgt ctaagaatcc ctatgaaacg
     1141 actgcggcgg ttatttatga aattatcgaa gagtctccga ctattcgcac tttgcgctta
     1201 cggccggaaa aaccgatgcc ttttcgcacc ggtcaattta tggaggtgac tgtgccgggt
     1261 attggcgagg gaccttttac gccttcatct tcgcattata ttgccgatac cctggatttt
     1321 acgattatga aaaccggatt cgtaacggaa tatattcatc gaaccaaagt cggtgatcgt
     1381 ctgggtttgc gtgggcctta tggcaagggt tatcccctga cggaattcaa aggcaagga
//
LOCUS       DAOSMP010000203         1434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_90379_length_1434_cov_8.732415, whole genome shotgun sequence.
ACCESSION   DAOSMP010000203 DAOSMP010000000
VERSION     DAOSMP010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90379_length_1434_cov_8.732415"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1434
                     /locus_tag="PKX09_11015"
     CDS             <1..>1434
                     /locus_tag="PKX09_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18391.1"
                     /translation="DGVTAEVGDEIGIFDGDICVGAKVITGPIGQYLALVAATDDPTT
                     SEKDGFTPGNPISYRLWDYSTQSEITNVSTVYTQGDAVFSSQGTAVLELHGSTEQPPS
                     PHFQVVYVGNPYLAMNIYLTAVTIDGAELGVGDEIGIFDDNICVGAKTLTGPIGQYLA
                     MVAATDDPTTPEKDGFTPGNPISYRLWDYSTQSEITNINTVYTMGEAIFSSQGTAVLE
                     LHGSSSAPTIQIIAPVADLTVPEDTSDFILTDLDNVFSQPPPGKTVTFSVASDTNAIG
                     VTLNESHVVWVSLAKNWNGVGRIYLRATMDEVTVTDTVQLTVTPVNDPPTAFILLSPA
                     QNATISQADITNFRWTRSYDLDRIGAIQYTLIIGRDNAFSVLDTTIYTGSDTSAAVTR
                     FSGGRHYWKVAAYNTRKDTVWGSGSNLTPWSFEISPVEIKVINPVADLEVAEDASDFQ
                     LADLDNVFSQPPPGKTVTFSVASDTNA"
BASE COUNT          362 a          340 c          345 g          387 t
ORIGIN      
        1 ttgatggcgt aacggcggaa gtcggcgatg aaattggtat attcgatggc gatatatgtg
       61 tcggcgcaaa ggttatcacc ggtccgattg gccaatatct agcattagtt gcggctactg
      121 acgacccaac cacatcggaa aaagacggct ttacgccggg aaatcccatc agctaccgct
      181 tgtgggatta ttcgactcag agtgaaatca ccaatgtaag tactgtttat acccaaggag
      241 atgcggtctt cagttctcag ggaaccgctg ttctggaact tcatggttct accgagcaac
      301 ctccatcacc ccatttccag gtggtttacg ttggtaatcc ctacctggcg atgaatattt
      361 atctcactgc ggttactatt gatggagccg agttgggagt cggcgatgaa atcggtatat
      421 tcgatgataa tatttgtgtc ggcgccaaga ctctcaccgg accgattggc caatatctag
      481 ctatggttgc ggctactgat gatcccacta caccggagaa agacggcttt acccctggca
      541 atcccatcag ctaccgcttg tgggattatt cgacacagag cgaaatcact aatattaata
      601 ccgtttatac gatgggagaa gcgattttca gttctcaggg aaccgctgtt ctggaactcc
      661 atggcagtag ttcagcacca acgattcaaa ttatcgcgcc ggtggctgac cttactgttc
      721 ccgaagatac ttcggatttt atattgactg atctagataa tgttttcagc cagccgccgc
      781 caggtaaaac cgttaccttt tctgttgcca gcgataccaa tgccattggc gttacgttga
      841 atgagtctca tgtcgtttgg gttagtctgg cgaaaaattg gaatggcgtt ggtcgcattt
      901 atctccgcgc tacaatggac gaggtaactg tcaccgatac cgtgcaactg accgttaccc
      961 cggtcaatga cccaccgacc gcattcattt tgctaagtcc ggcacagaat gcaacgattt
     1021 cacaagcgga tattaccaat tttcgctgga cacgcagtta tgatcttgat agaattggag
     1081 ccattcaata tacgctaata attggacgtg ataatgcttt ttcggtgcta gataccacta
     1141 tttatacggg aagtgatacc agtgcggcgg ttacccgatt ttccggtgga aggcattact
     1201 ggaaagtagc ggcttacaac actcgtaaag atacggtctg gggctccgga agcaacctaa
     1261 ctccctggtc ttttgaaatc agtccggttg agataaaagt cataaacccg gtagccgatt
     1321 tggaagttgc tgaagacgct tcggattttc agctggctga cctagataat gttttcagtc
     1381 agccgccgcc aggtaaaacc gttacctttt ctgttgccag cgataccaat gcca
//
LOCUS       DAOSMP010000204         1404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_93237_length_1404_cov_12.097109, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000204 DAOSMP010000000
VERSION     DAOSMP010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93237_length_1404_cov_12.097109"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PKX09_11020"
     CDS             complement(<1..727)
                     /locus_tag="PKX09_11020"
                     /inference="COORDINATES: protein motif:HMM:NF036499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18392.1"
                     /translation="MSKITKSLRNGILGILWVASLISSAYTQEAKFQQQSDYGLVCME
                     AENYTEIENPIEESYWELVTEPEGYSGIGAMQAMPIDQSFTRHKTLDDAMDSAPFMSY
                     TVNFITTGTHYVWLRACHIDGYDDSVWFGLDYLIYGEYPIQFRVSTDPEFDEQQYSNV
                     WHWISYLMADGDPKATIEVESPGVHTFNLYMREQAFKVDKIVLTTDADYIPNADAEEG
                     PEETLTTGTGLKLANSSVAYEFKL"
     gene            1208..>1404
                     /locus_tag="PKX09_11025"
     CDS             1208..>1404
                     /locus_tag="PKX09_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18393.1"
                     /translation="MIKINFRKISRHKSGDIDSRKRNNSTEWLADKIYYTIIKRTLFR
                     NILLGIVVFPLILMSIGCDDS"
BASE COUNT          438 a          238 c          242 g          486 t
ORIGIN      
        1 ctagtttaaa ttcataggca accgaggaat ttgctaattt taggcctgtt ccagtagtca
       61 gagtttcttc cggaccttct tctgcatcgg cgttaggaat gtaatcggcg tctgtagtaa
      121 gtacaatttt atctacttta aacgcttgtt cacgcatata aagattaaag gtatgtacgc
      181 caggtgactc aacttcaata gttgctttcg gatcgccatc agccattaaa tagctgatcc
      241 agtgccacac attggaatat tgttgctcat cgaactccgg gtcagttgat acccgaaact
      301 ggattggata ttctccatat attaaatagt caaggccaaa ccagacggaa tcatcataac
      361 catcgatgtg acaggcgcgt aaccacacat agtgggtgcc agttgtaatg aagttgactg
      421 tgtaactcat aaatggagcg ctatccatag catcatctaa agtcttatgc cgagtgaaag
      481 attgatcaat tggcatagct tgcattgctc caataccaga atagccttca ggctcagtaa
      541 ccagctccca atacgattct tcgatagggt tctcaatttc ggtataattc tctgcttcca
      601 tacatactag accataatca ctctgttgtt gaaatttggc ttcttgggta taagcggagc
      661 taattaaact ggctacccac aaaataccca atattccatt tctcaaagat tttgtaattt
      721 ttgacattgt aattctcctt cttataaatg tttaaaaatt ggcgttgctt aattaacctt
      781 atagcatctt tttttctgtc acctccttac ggacttgttt ttctttatca aacaaacctc
      841 gactcatacg ttaattcttt ctaattctca cctctggtaa ttatttattt tcatctgatt
      901 ttattttaaa atataaattg aaaaataaaa tagattattt gggttttgat ttgtataatt
      961 tgacacaaaa ctcgatttaa tcagcatagt cgaattttaa ttatctgact taatttgagg
     1021 ttattcagaa tactcgtgat atggtaataa ataattgata ttattaagga aggtaaagta
     1081 attcttgtgg tgatttcgta ccatgatcta ccatttgata aaatagggaa taaatagatc
     1141 tcatttattg tctaatagta gttttattaa atcagaattt aaatgcaaat accaaggaga
     1201 agttattatg attaaaatca attttagaaa aattagtaga cataagtccg gtgatattga
     1261 cagtagaaag cgaaacaatt cgacggagtg gttagcggat aaaatttatt ataccattat
     1321 taaaaggacg ttgtttagaa atattttatt gggtattgta gtatttcccc tcattttaat
     1381 gtctattgga tgtgatgata gtga
//
LOCUS       DAOSMP010000205         1401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_93515_length_1401_cov_9.384101, whole genome shotgun sequence.
ACCESSION   DAOSMP010000205 DAOSMP010000000
VERSION     DAOSMP010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93515_length_1401_cov_9.384101"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PKX09_11030"
     CDS             complement(<1..601)
                     /locus_tag="PKX09_11030"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HOU18394.1"
                     /translation="MKILKGIRISPGVAIGSAYYVETGKVAIPHTYIDQSEVEAELEK
                     FHRATQRATEELNQVRDMVMKHLDEEHARLIDAQIMTLADVDLVQKITDMVRQEHRNV
                     LWAYDAAMEWYENALLNTMNQFQQERLIDLQDIKKRMFRHLSTEEQLDLPQIPERAIY
                     VGERISPSELIQLHNQKILGIITRIGGMDSHSGILARAFG"
     gene            complement(606..872)
                     /locus_tag="PKX09_11035"
     CDS             complement(606..872)
                     /locus_tag="PKX09_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOU18395.1"
                     /translation="MVRQTVKILNRHGLHTRPATVLVTLCSRFKSDIFLIYKNVRVNA
                     KSILGVLVLAVEPNAEVTIEVDGPDEQEALAAIVELVANKFNTE"
     gene            complement(874..>1401)
                     /locus_tag="PKX09_11040"
     CDS             complement(874..>1401)
                     /locus_tag="PKX09_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOU18396.1"
                     /translation="QAFDLAYEKYDEIDLTDYLNMIARKTGRLLGLACQLAAIIADSS
                     PKVVNNLYLFGLELGQAFQIQDDLLELTADQAEMGKSLGSDLTAGKKTFPIIMALNEL
                     PAGQRADLLNFLKANAGNRRAIVQQLAKRGSIQQTEAMIARLFKNARERLHNMPDQLQ
                     SDLINLIELIAGRQS"
BASE COUNT          363 a          324 c          302 g          412 t
ORIGIN      
        1 ttccaaatgc tcgggctaga attcccgaat gtgaatccat cccaccgata cgagtaatga
       61 ttcccagtat tttctgatta tgtaattgaa ttagttcaga tggtgaaatc cgctcaccga
      121 catatattgc tctttcgggt atctgaggga ggtcaagttg ttcctccgtc gaaagatgac
      181 gaaacatacg ttttttgata tcctggagat caatcagacg ctcttgctga aattggttca
      241 tagtatttaa caaagcgttc tcataccact ccattgcggc atcataagcc cagagcacat
      301 tacgatgctc ctgccgcacc atatcggtga ttttttggac taagtctaca tcagctaatg
      361 tcattatttg agcgtcaatc agccgggcgt gttcttcatc gaggtgcttc attaccatat
      421 cgcgcacctg atttaattcc tccgttgcac gttgagtagc tcggtggaat ttttccagct
      481 cggcttcgac ttcagactga tctatataag tatggggaat cgcaactttt ccggtttcaa
      541 cgtagtatgc cgagccaata gcaacgcctg gggagattcg gatgccttta agaatcttca
      601 cttttttatt ccgtattgaa tttattagct accagttcaa ctatagcggc taaggcttcc
      661 tgttcgtcag gaccatcaac ctcgatagtg acctcggcgt tcggctcgac cgctaaaacc
      721 agtactccga gaatgctctt agcattgacg cgcacatttt tatagattag aaatatatcg
      781 gatttgaaac gggaacaaag ggtaaccaga acggtagccg gtcgagtatg cagaccatgc
      841 cgatttagaa ttttaacggt ttgtctgacc atcttaggat tgacgtcccg ctattaattc
      901 tattaaatta atcaagtcgc tttgtaattg atcaggcata ttgtgcagtc tttcacgggc
      961 atttttaaaa agccgggcga tcatagcttc cgtctgttga attgaaccgc gtttggccaa
     1021 ttgttgaacg atcgcccgcc gattaccggc attggctttg aggaaattaa gtaaatcagc
     1081 gcgctgaccg gctggcaatt cattcagcgc cataataata ggaaaagttt ttttccctgc
     1141 ggtgagatcg ctcccgagac ttttacccat ttcagcttgg tcagctgtca gctccaataa
     1201 atcgtcctga atctgaaagg cttgtcccaa ttccaagcca aaaagataga gattatttac
     1261 caccttcgga ctggaatcgg cgatgatcgc cgccagctga caggctaatc ccagaagtcg
     1321 gccggttttg cgagcaatca tattcagata atcagtgaga tcaatctcat catatttttc
     1381 ataagccagg tcgaatgcct g
//
LOCUS       DAOSMP010000206         1397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_93930_length_1397_cov_12.184799, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000206 DAOSMP010000000
VERSION     DAOSMP010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93930_length_1397_cov_12.184799"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PKX09_11045"
     CDS             <1..377
                     /locus_tag="PKX09_11045"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HOU18397.1"
                     /translation="ESRKQPFYIHHPEKRLLTMAGLYSRWVASDSKSYLTYTVITTPA
                     NSQLQFIHQRMPTILTPEEIDTWINCRQFDRQKALTLLQPSRMPLIAYPVSTFVNSVK
                     NNSPQCIQPINLTVEQTNLLND"
     gene            370..>1397
                     /locus_tag="PKX09_11050"
     CDS             370..>1397
                     /locus_tag="PKX09_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HOU18398.1"
                     /translation="MISHKIIINGIVQGVGFRPFVYNLARQYQLTGWVRNNSHGVEIV
                     AQGEPIQLARFAEIIQNSPPPRCRIERFEVTQFITAEKYLEFKIGESQNEQMKSVQIS
                     PDLDVCPDCRREMFDSSNRRYLYPFINCTNCGPRFTIIQDVPYDRQLTTMHEFKMCPE
                     CQCEYDDPSNRRFHAQPNACPVCGPRLQLLDNASRELLTGGSADANRAVFEKVAELLR
                     DGRIIAVKGIGGFHLACDARNEAAVTSLRQRKFREDKPFAVMFPSLPDIAKYCKYSRE
                     EARLLNSVPHPIVLLQKLPGRDVASAVAPGNHYLGCFLPYSPLHYLLMYFFPYPLVLT
                     SGNVSDEP"
BASE COUNT          355 a          325 c          318 g          399 t
ORIGIN      
        1 ctgaaagtcg taaacaacca ttctacattc atcatccgga aaagagattg ctgacgatgg
       61 ctgggcttta tagtcgttgg gttgcgtcgg atagcaagtc ataccttact tataccgtca
      121 tcaccactcc ggctaattcc caattgcaat ttattcacca acgaatgcct acgattctga
      181 cgccggaaga aattgacacc tggattaatt gccgccaatt tgatcggcaa aaagcgctga
      241 cgcttctcca gccgtcgcga atgccgctaa tcgcttatcc ggtttcgact ttcgtcaatt
      301 ccgtgaaaaa taattctccc caatgtattc agccgattaa tctaactgtc gagcagacca
      361 acctattaaa tgattagtca taaaataatc attaatggaa ttgtacaagg agtcggtttc
      421 cgaccatttg tctataatct tgctaggcaa tatcagctca ccggttgggt gcgaaataat
      481 tcgcacggcg tggaaattgt tgctcaggga gaaccaattc aactagctcg ttttgcagag
      541 ataattcaaa attctccgcc gccgcgctgt cgtatcgagc gcttcgaggt aacgcaattt
      601 ataacggctg agaaatattt ggaatttaaa attggtgaaa gccaaaatga gcaaatgaaa
      661 agtgtccaga tttctcctga tctggatgtt tgtccggatt gccgccgcga aatgttcgat
      721 tcgtccaacc ggcgttatct ttatcccttt attaattgca cgaattgtgg gccgcgtttt
      781 acgattattc aggatgtgcc atacgatcgc caactgacga cgatgcacga attcaaaatg
      841 tgtccggaat gtcagtgtga atatgacgat ccgtccaatc ggcgttttca tgctcagcca
      901 aatgcctgtc cagtctgcgg gccgcgtctg caattgcttg ataatgccag ccgcgaatta
      961 cttaccggcg gcagtgcaga tgctaaccgt gctgtttttg aaaaagttgc tgaactgttg
     1021 cgcgatggtc ggattatcgc ggtcaaaggt atcggcggtt ttcacttagc ctgcgatgcc
     1081 cgcaatgaag cggctgtcac gtctctacgt caacgaaaat tccgggagga taaaccgttt
     1141 gccgttatgt ttccaagtct tccggatatt gctaaatatt gtaaatacag tagggaggaa
     1201 gcgcggctat tgaatagtgt gcctcacccg attgttttac tgcaaaaatt gcctggcagg
     1261 gatgttgctt cggctgtggc acctggcaat cattatctcg gctgttttct accgtattcg
     1321 ccattgcact atttacttat gtattttttc ccgtacccgt tggtgcttac cagtggcaat
     1381 gtcagcgacg aaccaat
//
LOCUS       DAOSMP010000207         1385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_95189_length_1385_cov_15.353383, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000207 DAOSMP010000000
VERSION     DAOSMP010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95189_length_1385_cov_15.353383"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1358)
                     /locus_tag="PKX09_11055"
     CDS             complement(18..1358)
                     /locus_tag="PKX09_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOU18399.1"
                     /translation="MQKEYRYRCLAPNGKTVQGVVSATSKTKARQMVQKVADKHHLKI
                     VTLQPRRNFIYIVKSPEGKKIKGKQAAFSKEEVSAALAKMGYQNARIQPVLFDIKMKP
                     PFNSILMFIQLSSFLLNEQLSYDKILRMLADEETNPTLKETLRKIESELKKGREGTEV
                     FNQFADVFGRFPAYMLGLATKSGNMAEVYEATAKFMQRTADYKKSVRQATMTPMLTVL
                     VTIATVIYFVVSIFPATAEMFIKFGMELPPMTAATMKFSDFFGANWLWMFLAVVIPIV
                     GVFLWWRTPNGQVWRDKMLIKLPVIGPLLHKSSIEIFFRVFSAIYSGAESNIETLRAS
                     AEACKNAYIEKNIKEITIPKMLKGMALVPALEEAKVFNEATLSRLRSGSEVGNVLQSA
                     NMIALFYEKETTYKMQNLINSIQGFIGMFIALVVVLLTLVSAEVAFVSPPTSGF"
BASE COUNT          358 a          379 c          270 g          378 t
ORIGIN      
        1 aatgaccaat gaccacctta gaaccccgac gtcggtggcg agacaaatgc cacttcggcg
       61 gataccagcg tcagcaacac taccaccagc gcgataaaca tcccgataaa tccttgaatc
      121 gagttgatta aattctgcat cttgtaggtg gtttcttttt cataaaacaa agcgatcata
      181 ttagccgatt gaagcacatt tccgacttcc gaacccgacc ttaatctact taaggtcgcc
      241 tcattgaaaa cctttgcttc ctccagcgcc ggcaccagcg ccattccttt gagcattttg
      301 ggaatcgtaa tttccttgat attcttctcg atgtaggcgt tcttacaggc ttcggccgac
      361 gctcgtaaag tttcaatatt actctcggcg ccggaataga tcgccgagaa aacgcggaaa
      421 aatatctcga tactcgattt atgcaaaagc ggtccaatca ctggcaattt aatcaacatc
      481 ttatcccgcc agacttgccc gttcggcgtg cgccaccata aaaacactcc cacgatcggg
      541 atcacaactg ccagaaacat ccagagccaa ttagccccga aaaaatcact gaatttcatc
      601 gtcgccgcgg tcatcggtgg caattccatc ccgaatttga taaacatttc tgccgtggca
      661 gggaaaatgg aaaccacaaa ataaatcacc gtggcaattg tcaccagcac tgtcaacatt
      721 ggggtcatcg tcgcctgccg tacgctcttc ttataatcgg cggttctctg cataaatttc
      781 gccgtcgcct catacacttc cgccatatta ccgctcttgg tcgccagtcc gagcatataa
      841 gccggaaacc gtccaaaaac atcggcaaat tgattgaaca cttccgtgcc ttccctccct
      901 ttcttcaatt cgctctcgat cttccgtaga gtttccttca aagtcggatt ggtttcctca
      961 tccgccagca tccgcagaat cttatcgtaa gaaagttgtt cgttcaacaa aaaggaagaa
     1021 agctgaatga acatcaatat actattgaac ggcggtttca tcttaatgtc aaacagcacc
     1081 ggctgaatgc gggcattctg gtaacccatt ttcgccaggg cggcggatac ttcttcctta
     1141 ctaaatgccg cctgcttgcc tttgatcttc ttgccttccg ggctttttac tatatagata
     1201 aaattacgcc gtggttgaag cgttacaatt ttaaggtgat gtttatccgc taccttttga
     1261 accatctgcc gcgctttggt cttactagta gcggagacaa cgccctgaac ggtcttcccg
     1321 ttgggcgcta aacaacgata gcgatattct ttctgcataa aactcctttt ttatccttcg
     1381 acttt
//
LOCUS       DAOSMP010000208         1369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_96845_length_1369_cov_7.529680, whole genome shotgun sequence.
ACCESSION   DAOSMP010000208 DAOSMP010000000
VERSION     DAOSMP010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96845_length_1369_cov_7.529680"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PKX09_11060"
     CDS             <1..182
                     /locus_tag="PKX09_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18400.1"
                     /translation="VNTILIFWNPTVLVLNNQPWKIVDVIGAILAGVFVIVFVIVGIK
                     DAIRLDRLDRAEWEK"
     gene            complement(293..1312)
                     /locus_tag="PKX09_11065"
     CDS             complement(293..1312)
                     /locus_tag="PKX09_11065"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015168656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HOU18401.1"
                     /translation="MKKNDYLQKMVEHIDIKSFDARPILKAYGKMAFQARNLAVAADI
                     YDRMLKDEKCKVILTLAGSLFSAGLKEVVADMVENNMVDAIVSTGALIVDQDFFEALG
                     YKHYIGTPFSNDNELRELAIDRIYDTYIDEDELRVCDLTIAEIADSLPPRPFSSREFI
                     WEMGKYLDKVGIKTGKSVIYSAYQKGIPIFVPAFSDCSAGFGLIHHQWNRKGNMVTID
                     SARDFLELTKIKIAAAETGILMIGGGVPKNFTQDVVVATDVLGIEKPMHKYAVQLTVA
                     DERDGGLSGSTLKEASSWGKVDTKYEQMVFGEATVTMPLLVSYAYHQGNWKKRSEARF
                     SNLFV"
BASE COUNT          371 a          290 c          294 g          414 t
ORIGIN      
        1 tagtaaatac aattctaatt ttctggaatc ctaccgtgct ggtgttaaat aatcagccgt
       61 ggaagatagt tgatgttatt ggtgcgattc tcgctggggt ttttgttata gtttttgtaa
      121 ttgttggcat caaagacgca attagattag accgcctcga tcgtgccgaa tgggaaaaat
      181 aatcaagcaa ctagtgtaat taatctatta tttttggtcg gagaaagatg accaattgac
      241 cttttactcc cttactataa atccagtata acgactgttg gggttaagat tactaaacaa
      301 aaagattact gaagcgggct tccgagcgtt ttttccaatt accctgatga taagcataac
      361 tgaccagaag tggcattgtc accgtagcct cgccgaaaac catttgctcg tacttagtgt
      421 cgactttgcc ccatgaactg gcttctttga gggttgatcc agatagccca ccatcgcgtt
      481 cgtcagccac ggttaactga acagcatatt tatgcattgg tttttcgatt ccgagcacgt
      541 ctgtggctac aacaacgtcc tgagtaaaat ttttgggaac accaccgcca atcatcagaa
      601 tgcccgtttc tgcggcggcg attttgattt ttgtcagttc gaggaaatcg cgagcggaat
      661 caattgttac catattgcct ttgcgattcc attggtggtg aatcagtccg aaaccagccg
      721 aacaatccga aaaagccggt acaaaaattg gaatgccttt ctgataggct gaataaatga
      781 cggatttgcc ggttttgata ccaactttat cgaggtactt tcccatttcc cagatgaatt
      841 cgcgcgatga aaatggccgt ggtggcagag agtcggcgat ttcggcgatg gtcaaatcgc
      901 agacgcgcag ttcatcttca tcaatataag tgtcataaat gcggtcaata gccagttcac
      961 gtaattcgtt atcgttgctg aagggggttc cgatataatg tttataacca agtgcttcga
     1021 agaaatcctg atcgacgatc aaagctccag ttgaaacgat ggcatcaacc atattatttt
     1081 cgaccatatc agccacgacc tctttcaatc cggcgctgaa taatgacccg gcgagagtca
     1141 ggatgacttt acatttttcg tcttttagca ttcggtcata aatatcagcc gcgacggcta
     1201 aattgcgtgc ctgaaaagcc attttaccat aggctttcag aatcggccgc gcgtcgaatg
     1261 atttaatatc aatatgttca accatctttt gcagatagtc gttttttttc attgcattga
     1321 ctcctttgta taaatcatac ttaatgttcg ataaatgagt tttgccgct
//
LOCUS       DAOSMP010000209         1365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_97275_length_1365_cov_16.023664, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000209 DAOSMP010000000
VERSION     DAOSMP010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97275_length_1365_cov_16.023664"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PKX09_11070"
     CDS             complement(<1..326)
                     /locus_tag="PKX09_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOU18402.1"
                     /translation="MKKLYRSRKHRIIGGVCGGIAEYFEIDPVLVRLIWVILVIFGGM
                     GILAYIIAWIIIPNRDESNSTVEQPDQPSEPQSSTEVATNTSNKNLFWGIVLIVIGLL
                     IIANQF"
     gene            complement(346..1080)
                     /locus_tag="PKX09_11075"
     CDS             complement(346..1080)
                     /locus_tag="PKX09_11075"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HOU18403.1"
                     /translation="MQQIINHPWNVSIKEARAIQLQLAKEVILQQFTGNIARIWAVDV
                     SYGRFDRVGYGVLGWFVTADDSSGGQFDCQMIKIFTTISSVAFPYVPGYLSFREIPVL
                     LPLFQELPEKPDLVLVDGAGIAHPRGIGLAAHLGVIFNVPTIGCAKSRLFGNYTEPAN
                     ESGAFSQLTNDDNIIGFVLRTKKNTRPLFISPGHLTDLQSSLEIIKRFCGKYRIPEPL
                     RRVDFISKELRRNPDQFQSNIIYCNQ"
     gene            complement(1081..>1365)
                     /locus_tag="PKX09_11080"
     CDS             complement(1081..>1365)
                     /locus_tag="PKX09_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18404.1"
                     /translation="VIKPAPSFVQLAWNKLALFQATRYDDFIYTTQFHPEYTKEIMDW
                     YLKKNLALLTRQHISNPLHFATADEILKRNKSLPKSKLIIQNFLNLINSK"
BASE COUNT          404 a          288 c          275 g          398 t
ORIGIN      
        1 cagaattgat tggcaatgat tagcagacct ataacaatca gcacgattcc ccagaaaagg
       61 ttcttattcg acgtgttagt tgctacttca gttgatgact gtggttcgga aggttgatcc
      121 ggctgttcga ctgtcgagtt cgattcatct cgatttggaa taataatcca ggcaatgata
      181 taagccaata tgcccattcc gccaaaaata accagaatta cccagattag tcgtactaaa
      241 accgggtcaa tttcaaaata ctcagcaatg ccaccacaaa ctccgccaat aattcggtgc
      301 ttgcgactgc gataaagttt tttcattttc tacctcacct ctaatttatt gattacagta
      361 aattatattg ctttggaatt gatcgggatt ccgacgtaat tcttttgaaa tgaaatctac
      421 cctccgaagc ggttcgggaa tccgatattt cccacaaaat cgtttgataa tttcgagcga
      481 tgattggaga tcggtcaggt gaccgggtga aataaaaagc ggtctagtat tctttttcgt
      541 tcgcaggaca aatccgataa tgttatcatc attagtcaat tgagaaaatg ccccgctttc
      601 gttagccggt tcggtataat taccaaatag ccgcgacttg gcacaaccga ttgtaggcac
      661 gttgaaaatt actcccagat gggcggctaa tccgattccg cgtgggtgag caattccagc
      721 cccatctacc aacaccaaat caggcttttc cggcaattcc tgaaacagag gtaaaagcac
      781 cggaatttcg cgaaaactca gatatccggg aacatacgga aaagcgactg aactaatcgt
      841 cgtaaaaatc ttgatcattt ggcaatcaaa ttgcccgcca gaagaatcat cagcagtgac
      901 aaaccagccc aagaccccat atccgactct atcaaagcgt ccataagaga catcaactgc
      961 ccatattctg gctatattac cggtgaattg ttggagaatc acctccttag ctagttgtaa
     1021 ctgaatagct ctggcttctt tgattgatac gttccaggga tgatttataa tttgttgcat
     1081 ttatttactg ttaatcaaat taagaaaatt ctggataatc agtttggact ttggtaatga
     1141 tttgttccgc ttgagaattt catcagctgt tgcgaaatgg aggggattcg aaatatgttg
     1201 tcgtgttaat agcgcaaggt tctttttcaa ataccaatcc attatttcct tagtatattc
     1261 cggatgaaat tgggtagtat aaataaaatc gtcatagcgc gtcgcctgaa atagcgccaa
     1321 tttattccag gctaattgga caaaactcgg ggctggtttt ataac
//
LOCUS       DAOSMP010000210         1343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_99595_length_1343_cov_11.922360, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000210 DAOSMP010000000
VERSION     DAOSMP010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99595_length_1343_cov_11.922360"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PKX09_11085"
     CDS             complement(<1..680)
                     /locus_tag="PKX09_11085"
                     /inference="COORDINATES: protein motif:HMM:NF022932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU18405.1"
                     /translation="MDIFQDNVLHKMMQLGFTNYEARTYLGLLKLSPATGYEISQLAH
                     VPRSVVYSVLKQLESRGIVISIHDKPRRYIPLAPDEMLKRMETDYASRVSNLKDDLAE
                     LSNKSGTEGFWNLRGYQSLMQTCETLIREAHKSIYISGWQREVSLLREHLLNACKRNV
                     FVVIFSFNEINPPIGVTLAYGIPEEKLEHYWDRKLILVTDSKNLIMGPANQKEDEQAI
                     WTQNKAALT"
     gene            complement(701..>1343)
                     /locus_tag="PKX09_11090"
     CDS             complement(701..>1343)
                     /locus_tag="PKX09_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOU18406.1"
                     /translation="MRQSYGASIIIVVLGLVLMLIIKNINEIWGWITMSIGAGLIVPQ
                     LLRWYWWRLNGYGYTIGMAAGMLASIIWKIIAPEATAEYWSFLFAAGISTVATVIGTL
                     LTEPTDEKVLDNFYRVTRPFGFWGPIRAKLPEKLISQINRENRRDIISIFFAVPWQVC
                     LFLMWMVLVMKRYDTFLSLLLIVVTLSTILYFTWFRHLATEVNIESDADSSSG"
BASE COUNT          396 a          287 c          273 g          387 t
ORIGIN      
        1 gttagagccg ctttattctg agtccagatg gcttgctcat cttctttctg attagctggt
       61 cccataatta aatttttcga atcggttacc aaaattaatt tccgatccca ataatgttcc
      121 agtttttctt ccggaatgcc ataagccaag gttactccga ttggcggatt aatttcatta
      181 aaagaaaaaa ttacgacaaa aacattgcgt ttgcaggcat ttagtaagtg ctctctaaga
      241 agcgaaacct cacgttgcca gccggaaatg tagatagatt tatgagcttc gcggattaac
      301 gtctcacagg tttgcattaa agattggtat ccacgtaagt tccagaatcc ctcggtgcct
      361 gatttattac tgagttcggc taaatcgtct ttaaggttcg atactcgact ggcataatcg
      421 gtctccatcc gcttcagcat ctcgtccggt gccaaaggga tatatcggcg cggtttatca
      481 tgaatcgaaa ttacgatacc tcgcgattct aactgtttta ataccgagta aactacggag
      541 cgaggaacgt gagccaattg actgatttca taaccggtag caggactcaa tttcagtagt
      601 ccgaggtaag tgcgggcttc atagttagta aagcccagtt gcatcatttt atgaagaaca
      661 ttatcctgaa aaatatccat ttttttcttt ctattttagg ttaccccgaa gatgaatcgg
      721 catcggattc gatgtttact tctgtcgcaa gatgacgaaa ccaggtaaag tataatattg
      781 ttgatagtgt tactacaatc agtaataagc taaggaaagt atcatagcgt ttcatcacta
      841 gaaccatcca catcaaaaac aggcacacct gccaaggcac ggcgaagaaa attgagatta
      901 tatcgcgtcg attttcacga ttgatttgag atatcagttt ttcgggtaat ttagctcgga
      961 ttggtcccca aaaaccaaat ggacgggtaa ctcggtagaa attatccaat actttctcgt
     1021 cagttggttc ggttaaaagg gtgccgataa ccgtagcgac ggtactaatt ccggcggcaa
     1081 atagaaatga ccagtattcg gcggttgctt caggggcgat aattttccag atgatcgaag
     1141 ctagcattcc ggcagccata ccgatagtat aaccataacc atttagtcgc caccaatacc
     1201 agcgcagtaa ttggggcaca atcaagccgg ctcctatact catagtaatc cagccccaaa
     1261 tttcattaat attcttaata ataagcatta gaaccagtcc aagaactaca ataattatcg
     1321 aggctccata gctttgccgc att
//
LOCUS       DAOSMP010000211         1331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_100950_length_1331_cov_18.426332, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000211 DAOSMP010000000
VERSION     DAOSMP010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100950_length_1331_cov_18.426332"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PKX09_11095"
     CDS             complement(<1..272)
                     /locus_tag="PKX09_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOU18407.1"
                     /translation="MIQPLFWENERLLIVDQTRLPFEYKLIEIHDDHEMADAIRRMVI
                     RGAPAIGIAAAYGLALGLKKFILGNHEEFFRQLEIISNRLYETRPTA"
     gene            complement(294..1199)
                     /locus_tag="PKX09_11100"
     CDS             complement(294..1199)
                     /locus_tag="PKX09_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002619231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOU18408.1"
                     /translation="MKKSILVVGSVAFDDIETPVGKRFNALGGSAVYFSLAASQFSQV
                     NLVGVAGEDYPKAAIDLLNRQGIDTTGLEIAPGPTFRWGGRYHQDLNNRDTLYTHLNV
                     FANFSPKIPSIYQKTPVVFLGNIQPTLQMEVLNQLPQPEFIGLDTMNLWINTTRAELM
                     KIISRVNLLVINDSELEELTGTVNLFKGLKYLHKIGVRYIVIKQGEYGAMLSAAEGLF
                     FTPAYPVEEVVDPTGAGDSFAGGLFGYLGTCEKIDYDNLKKAVVYGSVMGSFCVENFS
                     IDGLLNLNPTKIAKRFRELRRLVKA"
BASE COUNT          382 a          302 c          285 g          362 t
ORIGIN      
        1 gcggttggac gggtttcata aagtcggttg gaaattattt ctaattggcg gaaaaattct
       61 tcatgattac ctaagataaa ctttttaagc cccagcgcta aaccataggc ggcggcaatg
      121 ccgatagctg gagcaccacg aataaccata cggcgaatcg cgtccgccat ttcgtggtcg
      181 tcatgaattt cgatcagttt atattcaaac ggcaggcgcg tctgatctac aatcagcagt
      241 cgttcatttt cccaaaataa gggttggatc ataagcaatt agtccgattg agctcaggct
      301 ttcactaacc ggcgcaattc gcgaaatcgc tttgcgattt tggtaggatt aagattcaat
      361 aaaccatcta tactgaaatt ttcgacacag aatgaaccca taacactgcc gtaaaccacc
      421 gcctttttta gattgtcgta atcaattttt tcgcaggtgc cgagataacc aaacagtcca
      481 cccgcaaaac tatcaccggc tccggtagga tctacaacct cttcaactgg ataagccgga
      541 gtgaaaaata agccttcagc cgcactgagc attgcaccgt attctccctg cttgataacg
      601 atataccgta ctccgatctt atgcaagtat tttaaacctt tgaacaggtt aactgtacca
      661 gtcaattcct ccagctcact atcgttgatg accagaaggt taactcgcga tatgattttc
      721 attagttcgg cgcgcgtggt attaatccac aaattcattg tatccagtcc gatgaattcc
      781 ggctggggta gttgattaag cacctccatt tgtaaagtcg gctgaatgtt gccaaggaag
      841 acgactggtg tcttttgata aatggaggga attttcggac tgaaattagc gaagacatta
      901 agatgagtat aaagagtgtc gcggttattg aggtcttggt gataacgccc gccccagcga
      961 aaagtcggcc ccggagcaat ttctaaaccg gtagtatcaa ttccctgacg gttcagcagg
     1021 tcaatggcgg ctttggggta atcttcaccg gcgacgccga ctaaatttac ttgtgagaat
     1081 tgagaagccg ctaaactgaa ataaacggcc gaaccaccca gggcattgaa acgctttccg
     1141 accggggttt cgatatcatc gaatgcaacg gaaccaacta ccagaataga tttcttcaaa
     1201 actatttcct tattttatta tcaattaacc accgaagcta aaaatacttt ggcgcttttg
     1261 caatatggtc aggtaggttt ataataaacg ataagcattt gattccggta gtccaatatc
     1321 ctggatcaac c
//
LOCUS       DAOSMP010000212         1263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_109087_length_1263_cov_15.233444, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000212 DAOSMP010000000
VERSION     DAOSMP010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109087_length_1263_cov_15.233444"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PKX09_11105"
     CDS             <1..356
                     /locus_tag="PKX09_11105"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOU18409.1"
                     /translation="QVTDYTPNRVVLNVETERPAFLVLADAYYPKGWTARVNGVETPI
                     YQVNHVLRGVSVPAGSYAVEFKFLPRSYQIASRISTISCDIVWLSLLGVLIYQNREKI
                     KNLKKKRPTPAKSNR"
     gene            399..>1263
                     /locus_tag="PKX09_11110"
     CDS             399..>1263
                     /locus_tag="PKX09_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18410.1"
                     /translation="MKINIYRHSGNIKRLLLVLAIVMIFALLNYTQKIVTRLRQDSAN
                     LIRFYADIYAKAAMDMSGDDFSFIFEEIIQKTSTPMILSDAEAMEPTAWRNIGVPDND
                     RSPATLAKVKKIMQEMDRENQPIPLKYEDRVLQYIHFGDTNMIRQLRRLPYIEIAIVG
                     LFILLGYVGFSLIRENEKRSIWVGMAKETAHQLGTPLTALMGWLELLKDEIGDNANIS
                     EIARDIQRLEKVANRFSQIGSRPVLKPTNINSVINDAISYYRRRLPQLGPGISLEFIP
                     SGDYIVNLNADL"
BASE COUNT          337 a          280 c          296 g          350 t
ORIGIN      
        1 ttcaagtaac cgattatact cccaatcgcg tggtgctaaa cgtcgaaacc gagcgcccgg
       61 cctttttagt tttggcggat gcttactatc cgaaaggttg gacggctcgc gtcaacggcg
      121 ttgaaacacc gatttatcag gtcaatcatg ttttgcgagg ggtgtcggtt ccagcgggaa
      181 gttatgcagt ggaatttaag tttttaccgc gtagttatca gatagcgtcc cggatttcta
      241 ctatttcttg tgacatcgtc tggctgagcc tgcttggagt gttgatttat caaaatcgag
      301 agaagatcaa aaaccttaag aaaaagcgcc caacacctgc caaaagcaac cgatgatttt
      361 accaatgcaa ctaaaatgat tattattggc atagttccat gaaaataaat atttatcgtc
      421 attctggcaa tatcaagcgc ttgctgttag ttctggcaat tgttatgatt ttcgccttgc
      481 tgaattatac ccagaaaatc gtcacgcgcc tgcgccagga ttccgccaat ttgatccggt
      541 tttatgctga tatttatgcc aaagccgcta tggatatgag tggcgacgac tttagtttta
      601 tttttgaaga aataatccag aaaacctcga ctccaatgat tctttcggat gccgaggcta
      661 tggaaccaac cgcctggcgc aacattggcg ttccggataa cgatcgttcg cccgctactc
      721 tggctaaagt caagaagata atgcaagaaa tggatcggga aaatcagcca attccactga
      781 aatacgaaga tcgagtattg caatatattc atttcggcga taccaatatg attcgccaac
      841 tgcggcgctt gccctatatc gaaattgcca tcgtcgggct gtttattttg ctcggatatg
      901 tcggcttttc gttaatccgg gaaaatgaga agcgctcaat ctgggttggt atggcaaaag
      961 aaaccgcgca tcaattaggc acgccgctga ccgcgctgat gggttggttg gaattgctga
     1021 aggatgaaat cggcgacaat gccaatatca gcgaaattgc gcgcgacatt cagcggttgg
     1081 agaaagtggc caatcgtttt tcgcagatcg gcagtcgccc ggttctaaaa ccaactaaca
     1141 ttaattcggt cattaacgat gccatttcgt attatcggcg tcgtttaccg caactgggac
     1201 cgggcatcag tctggaattt atcccatctg gcgattacat cgtcaacctg aatgcggatt
     1261 tat
//
LOCUS       DAOSMP010000213         1263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_109092_length_1263_cov_9.238411, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000213 DAOSMP010000000
VERSION     DAOSMP010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109092_length_1263_cov_9.238411"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1263)
                     /locus_tag="PKX09_11115"
     CDS             complement(<1..>1263)
                     /locus_tag="PKX09_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18411.1"
                     /translation="GDASNDIFPSENTKFISVQSDGTVITDINTVQVRPTAKVAGYDQ
                     QNSELSLDHLAGPATGSIQYKILDETLLNGHTYKVTFTDTRTDGIDNDGDWNYLTYDV
                     GSDGKPGTNDYGEGDGVPTSGEPNFEWNDEDEFAQITSTYSVLDKTGVEEFINLDTSY
                     VWLTHQNIQYDDILIASESDPNNYLSLSKFEIDTLRGKIKIKADSNLKSGSYRIFYKY
                     YPVFKSPNIQYSPYMKETKDSDIFDGVELVFSNPWDIKMIKSKSGWNSTDRIIDFTFR
                     VDLAKLEGDTIVGVQYPGIYELRVGDSSEVLYETPKEFLKSIYPSFSDAIIRTLVKKT
                     NFYLFNVLDSIKIPFLYNGRDLIKNSYGIYEITPESLIESFFPKPDSSTYQYSWVINF
                     PPVSDKVTPPRFLKGDILHIETEIPFRN"
BASE COUNT          390 a          248 c          208 g          417 t
ORIGIN      
        1 cattacggaa ggggatttcc gtttcaatat ggagaatgtc accttttaga aaccggggcg
       61 gtgtgacttt atctgaaaca ggcggaaagt ttatcaccca ggaatattga taggtactac
      121 tatccggttt aggaaaaaac gattcaatca agctctctgg cgttatttca taaattccat
      181 aactgttttt tattaaatca cgaccattat aaagaaaagg tatctttata gaatccaaca
      241 cattaaatag ataaaaatta gtcttcttta ccagggtcct tataattgca tctgagaaac
      301 taggatagat ggactttaag aattctttgg gagtttcata taatacttcg ctactatcac
      361 cgactcgcag ctcatatatt ccgggatatt gtactccaac tatggtatca ccttccaatt
      421 tagccagatc aactctaaag gtaaaatcaa taattctatc agtcgaattc cagcccgact
      481 tagatttaat cattttaata tcccacggat tactaaacac taactcaacg ccatcgaaaa
      541 tatcggagtc ttttgtttct ttcatataag gactatactg aatgtttgga cttttaaaga
      601 caggataata cttataaaat atacgataac taccactttt taggttacta tccgctttta
      661 ttttaatttt tcctcgtaag gtatctattt cgaatttact taatgataaa tagttattcg
      721 gatctgactc actggcaatt aaaatatcgt catattgaat attttgatgg gtaagccaaa
      781 cataagaagt gtcaagattg ataaattctt cgacgcctgt tttatctaaa acagagtaag
      841 tggatgtaat ttgagcaaat tcatcttcat cattccattc aaaattcggc tcaccgctcg
      901 taggtacgcc atccccttct ccataatcat ttgtacctgg tttgccatca gagccaacat
      961 cgtaagttaa ataattccaa tcgccatcat tatcaatccc atcggttcgg gtatcggtaa
     1021 aagtcacttt ataagtatgt ccattcaaaa gtgtttcatc aagaatctta tattggatgc
     1081 taccggtcgc cggtcctgcc agatgatcta agctaagttc actattttgt tggtcatagc
     1141 cggcaacttt cgcagttggt ctaacttgaa cggtgtttat atcggtaatt actgtcccat
     1201 ctgactgtac cgaaataaac ttggtatttt ccgatggaaa aatatcatta ctggcgtcac
     1261 cat
//
LOCUS       DAOSMP010000214         1254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_110298_length_1254_cov_9.414512, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000214 DAOSMP010000000
VERSION     DAOSMP010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110298_length_1254_cov_9.414512"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1254)
                     /locus_tag="PKX09_11120"
     CDS             complement(<1..>1254)
                     /locus_tag="PKX09_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18412.1"
                     /translation="GDASNDIFPSENTKFISVQSDGTVITDINTVQVRPTAKVAGYNE
                     QNSELALKHLEGPATGNIQYKIIDETLLNGHIYKVNFTDTRTDGIDNDGDWNYLTCDV
                     GSDGKPGTNDYGEGDGVPTSGEPNFEWNDEDEFAQITSTYSVLDKTGVEEFINLDTSY
                     VWLTHQNIQYDDILIASESDPNNYLSLSKFEIDTLRGKIKIKADSNLKSGSYRIFYKY
                     YPVFKSPNIQYSPYMKETKDSDIFDGVELVFSNPWDIKMIKSKSGWNSTDRIIDFTFR
                     VDLAKLEGDTIVGVQYPGIYELRVGDSSEVLYETPKEFLKSIYPSFSDVIIRTLVKKT
                     NFYLFNVLDSVKIPFLYNGGIKNSYGIYEITPGGLIESYIPKPDSTYQYSWVINFPPI
                     SDKVTPPRFLKGDILHIETEIPFRN"
BASE COUNT          388 a          247 c          197 g          422 t
ORIGIN      
        1 cattacggaa ggggatttcc gtttcaatat ggagaatgtc accttttaga aaccgtggtg
       61 gtgtgacttt atctgaaata ggtggaaaat ttatcaccca ggaatattga taagtactat
      121 ccggtttagg aatatacgat tcaatcaagc cacctggcgt tatttcataa attccataac
      181 tgttttttat tccaccatta taaagaaaag gtatctttac agaatccaac acattaaata
      241 gataaaaatt agtcttcttt accaaggttc ttataattac atctgagaaa ctaggataga
      301 tggactttaa gaattctttg ggagtttcat ataatacttc gctactatca ccgactcgca
      361 gctcatatat tccgggatat tgtactccaa ctatggtatc accttccaat ttagccagat
      421 caactctaaa ggtaaaatca ataattctat cagtcgaatt ccagcccgac ttagatttaa
      481 tcattttaat atcccacgga ttactaaaca ctaactcaac gccatcgaaa atatcggagt
      541 cttttgtttc tttcatataa ggactatact gaatgtttgg acttttaaag acaggataat
      601 acttataaaa tatacgataa ctaccacttt ttaggttact atccgctttt attttaattt
      661 ttcctcgtaa ggtatctatt tcgaatttac ttaatgataa atagttattc ggatctgact
      721 cactggcaat taaaatatcg tcatattgaa tattttgatg ggtaagccaa acataagaag
      781 tgtcaagatt gataaattct tcgacgcctg ttttatctaa aacagagtaa gtggatgtaa
      841 tttgagcaaa ttcatcttca tcattccatt caaaattcgg ctcaccgctc gtaggtacgc
      901 catccccttc tccataatca tttgtacctg gtttgccatc agagccaaca tcgcaagtta
      961 aataattcca atcgccatca ttatcaatac cgtcggttcg agtatcggtg aaattcactt
     1021 tatagatatg cccatttaat agtgtctcat caataatttt gtactgaata ttaccggttg
     1081 ccggcccttc caggtgcttc aaggctaatt cactattttg ttcattataa ccggcaactt
     1141 tcgcagttgg tctaacttga acggtgttta tatcggtaat taccgttcca tctgactgca
     1201 ccgaaataaa cttggtattt tccgatggaa aaatatcatt actggcgtca ccat
//
LOCUS       DAOSMP010000215         1241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_112014_length_1241_cov_12.724283, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000215 DAOSMP010000000
VERSION     DAOSMP010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112014_length_1241_cov_12.724283"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PKX09_11125"
     CDS             complement(<1..610)
                     /locus_tag="PKX09_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOU18413.1"
                     /translation="MEWFGELSPVFQAFLATCFTWLVTAGGAGLVIFFKEVSQRLLDA
                     ALGFAAGVMIAASFWSLLAPAIEMAGASSTPAWIPVAIGFLLGGAFLKLVDRLLPHLH
                     LGLPLDQAEGIKTHWQRTVLLVLAVTIHNIPEGLAVGVAFGAAGAGLPSASLAGAVAL
                     ALGIGIQNFPEGVAVTVPLRREGLSIGKSFFYGQLSGFVEVIS"
     gene            complement(631..>1241)
                     /gene="pgi"
                     /locus_tag="PKX09_11130"
     CDS             complement(631..>1241)
                     /gene="pgi"
                     /locus_tag="PKX09_11130"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252044.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOU18414.1"
                     /translation="YLQQADMESNGKRVDRNGRVVNYQTGPIVWGEPGTNGQHAFYQL
                     IHQGTKLVPCDFLAPAISHNPVGDHHNILLSNYFAQTEALMNGKSEEEVARELKSKGL
                     TEAEIQRQLPYRVFPGNKPSNSILFKKLTPRTLGALIVMYEHKIFTQGIIWNIYSFDQ
                     WGVELGKQLAGRILPELADDQPITSHDSSTNGLINAWKRLRK"
BASE COUNT          359 a          335 c          256 g          291 t
ORIGIN      
        1 cggaaatgac ttccacaaaa ccggaaagtt gaccgtaaaa aaaactcttg ccgatcgaaa
       61 gtccctcccg acgcagtgga accgtaaccg ccacaccttc tgggaaattt tgaatgccaa
      121 tgcccagagc cagggcaact gcaccagcta gtgaagccga aggcaagccg gctcccgccg
      181 cgccgaacgc tactcccact gccagaccct ccggaatgtt atggatcgtg accgccagaa
      241 ctaacagtac cgtcctttgc cagtgagttt taatgccctc cgcctgatca agcggtaacc
      301 ctaaatgcaa gtgaggaagc agacgatcaa ccaatttcaa aaaagcgccg cccaacaaaa
      361 atccgattgc cactggaatc caggccggag tacttgatgc acccgccatt tctattgccg
      421 gagccaatag cgaccaaaag ctagcggcta tcattactcc agcggcaaaa ccaagtgcgg
      481 catctaatag gcgttgactg acttccttga aaaaaatcac taacccagcg ccgccagccg
      541 tcaccaacca agtaaaacag gttgccagaa atgcttgaaa aacgggactt aattcaccaa
      601 accattccat aaatatcctc gttaaggcga ttacttacgg agtctcttcc aggcattaat
      661 caatccatta gttgagctat cgtgactggt aatcggctga tcatccgcaa gttccggcag
      721 aatccgtccc gccaattgct taccaagttc gacaccccat tgatcgaaac tataaatatt
      781 ccagattatt ccctgagtga aaatcttatg ctcatacata acaattagag cacccaacgt
      841 tcgcggagtt aattttttaa aaaggattga attgcttggc ttatttccag gaaaaacacg
      901 atagggcaac tgccgctgaa tttcagcctc agtcaaacct ttggatttca attcccgagc
      961 aacttcttcc tcgcttttgc cattcatcaa ggcttcggtc tgggcgaaat aattcgataa
     1021 tagaatatta tgatgatctc caaccggatt atgcgaaata gctggcgcta gaaaatcgca
     1081 aggaacaagc ttcgttcctt gatggataag ttgataaaaa gcatgctgtc cgtttgtgcc
     1141 aggttcgccc cagacaattg gcccggtttg ataattgact actctgccgt tgcgatcaac
     1201 tcgcttgccg ttactctcca tatccgcctg ttggagataa g
//
LOCUS       DAOSMP010000216         1241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_112016_length_1241_cov_8.491568, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000216 DAOSMP010000000
VERSION     DAOSMP010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112016_length_1241_cov_8.491568"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /gene="pgi"
                     /locus_tag="PKX09_11135"
     CDS             <1..611
                     /gene="pgi"
                     /locus_tag="PKX09_11135"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252044.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOU18415.1"
                     /translation="YLQQADMESNGKRVDRNGKVVNYQTGPIVWGEPGTNGQHAFYQL
                     IHQGTKLIPCDFLAPAISHNPVGEHHNILLSNYFAQTEALMNGKSEEEVARELKSKGL
                     TESEVQRQLPYRVFPGNKPSNSILFKKLTPRTLGALIAMYEHKIFTQGIIWNIYSFDQ
                     WGVELGKQLAGRILPELTDDQPITSHDSSTNGLINAWKRLRK"
     gene            632..>1241
                     /locus_tag="PKX09_11140"
     CDS             632..>1241
                     /locus_tag="PKX09_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOU18416.1"
                     /translation="MQWFGELSPVFQAFLATCFTWLVTAGGAGVVIFFKEVSQRLLDA
                     ALGFAAGVMIAASYWSLLAPAIEMAGASGSPAWIPAAVGFLLGGAFLKLVDRLLPHLH
                     LGLPLDQAEGIKTNWQRTVLLVLAVTIHNVPEGLAVGVAFGAAGAGLPSASLAGAVAL
                     ALGIGIQNFPEGAAVTVPLRREGLSIGKSFFYGQLSGFVEVIS"
BASE COUNT          284 a          260 c          344 g          353 t
ORIGIN      
        1 cttatctcca acaggcggat atggagagta acggcaagcg agttgatcgc aacggaaaag
       61 tagtcaatta tcagaccggg ccaattgtct ggggcgaacc aggtacgaac ggacagcacg
      121 ctttttatca acttattcat caagggacca agcttattcc ctgcgatttt ctagcgccag
      181 ccatttcgca taatccggtt ggagaacatc ataatattct attatcgaat tatttcgctc
      241 agaccgaagc cttgatgaac ggcaaaagcg aggaagaagt tgcccgggaa ttgaaatcca
      301 aaggtttgac tgagtctgaa gttcagcgcc agttgcccta tcgtgttttt cctggaaata
      361 agccgagcaa ttcaatcctt tttaaaaaat taactccgcg aacgctgggt gctctaattg
      421 ctatgtacga gcataagatt ttcacccagg gaataatctg gaatatttat agtttcgatc
      481 aatggggtgt cgaacttggt aagcaactgg ctggacggat tctgccggaa cttacggatg
      541 atcagccgat taccagtcac gatagctcaa ctaatggact gattaatgcc tggaagaggc
      601 tccgtaagta atcgccttaa tgaggatatt tatgcaatgg tttggtgaat taagtcccgt
      661 ttttcaagca tttttggcaa cctgttttac ttggttggta acggctggtg gtgccggagt
      721 agtgattttt ttcaaggaag tcagtcaacg cctattagat gccgcactgg gttttgccgc
      781 cggggtgatg attgccgcca gttattggtc gcttttggct ccggcaatag aaatggcggg
      841 tgcatcgggt agtccggctt ggataccggc ggcggtcgga tttttattag gtggcgcttt
      901 tttgaaattg gttgatcgtc tgcttcccca tttgcattta ggtttgccgc ttgatcaggc
      961 ggaaggtatt aaaactaact ggcaaaggac ggtgctttta gtgctggcgg tcacgatcca
     1021 caacgttccg gagggtttgg cagtaggagt ggctttcggc gcggcaggag ccggcttgcc
     1081 ctcggcttct ctggctggtg cggttgctct ggcgctgggc attggcattc aaaattttcc
     1141 ggaaggtgcg gcggttacgg tgccactgcg gcgggagggg ctttcaatcg gcaagagttt
     1201 tttttacggt caactttccg gttttgtgga agtcatttcc g
//
LOCUS       DAOSMP010000217         1237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_112590_length_1237_cov_10.319797, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000217 DAOSMP010000000
VERSION     DAOSMP010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112590_length_1237_cov_10.319797"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PKX09_11145"
     CDS             <1..897
                     /locus_tag="PKX09_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOU18417.1"
                     /translation="FPGLLFSAVLGLLVGWVDRKVSARVQFRVGPPLFQNFNDFFKLL
                     GKETIIVKESLHSLFVLSPLVAFGVTTLITTMLGVALFYGIGFGGDLLVIMYLLMIYS
                     VMVILGGSATGNVYSSLGAGREIKLLLADELAFILVCLVPIIKSGYHLKLEEILSAQV
                     AEGMFIGSFSGALAFIVGLLCIQAKMTLPPFHIPEAETEIVEGPLMEYSGPLLGFWKL
                     NQAMQWVAFPFLLILLFLGGFSEIGWGVLWSVLKYLGIVIVMVLIKNTNPRVRIDTAL
                     KFFWKYLTPVALLAVILAVLGV"
     gene            899..>1237
                     /locus_tag="PKX09_11150"
     CDS             899..>1237
                     /locus_tag="PKX09_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HOU18418.1"
                     /translation="MGWYKRRLSKSLWVFHVSGSPCNNCDIEILDLLTPRYDVERFGI
                     KLVGSVRHADVLLVTGVFNKKVAPKIKKLYEQAPKPIFVVAVGTCVSGGCCFKDSYNI
                     VPSREAVIPID"
BASE COUNT          276 a          242 c          327 g          392 t
ORIGIN      
        1 tttccaggat tattgtttag tgcggttctg ggattattag tcgggtgggt tgatcgcaaa
       61 gtcagcgcgc gggtgcaatt tcgcgttggc ccgccgcttt tccagaattt caacgatttt
      121 ttcaaattac tcggcaaaga aactataata gtaaaggaaa gcctgcattc tctttttgtg
      181 ttgtcgccgc tggtagcatt tggcgtaacg acgttaatca cgacaatgct gggcgtcgcc
      241 cttttttatg gaattggctt tggcggtgac ttgctggtga taatgtactt attgatgatt
      301 tattcggtta tggtcattct gggcggatcg gctaccggca atgtctattc cagtctcggg
      361 gcggggcgcg agatcaaact gcttcttgcc gacgaactgg ctttcatttt agtttgcctg
      421 gtgccaataa ttaaatcggg ctaccaccta aaacttgagg aaatactcag cgctcaggtg
      481 gcggagggaa tgttcatcgg ctcgttttcc ggagctctgg cattcattgt cgggctttta
      541 tgtattcaag ctaaaatgac tctgccaccg tttcatattc cggaagccga aactgaaatt
      601 gtcgaagggc ctctgatgga atattccggg ccattgctcg gtttctggaa attgaatcaa
      661 gctatgcagt gggtggcatt tccattttta ttaattttgt tatttctcgg tggatttagc
      721 gaaattggct ggggcgtttt atggtcggta ttgaaatatc tcggaatcgt catagtgatg
      781 gttttgatta aaaataccaa tccgcgcgtt cggattgata cagcgttaaa atttttctgg
      841 aaatatctga cgccagtggc tttgctggcg gtcattttag cggttttagg agtgtaaaat
      901 gggatggtat aaacgtcgtc tttcgaaatc gctgtgggtt tttcatgtca gcggttcgcc
      961 ctgcaacaat tgcgacatcg agattctcga tttactgacg ccgcgctatg acgtcgaacg
     1021 gttcggtatc aagttggttg gttcggtacg gcatgccgat gtgcttctgg tgacgggtgt
     1081 gttcaataaa aaagttgcgc ccaagattaa aaagctctat gaacaagcac ccaaaccgat
     1141 tttcgtagtg gcggtaggta cttgtgtttc tggcggttgt tgtttcaaag atagctacaa
     1201 tattgtgccg tcacgagaag cggtcatacc gattgac
//
LOCUS       DAOSMP010000218         1222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_114627_length_1222_cov_23.756641, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000218 DAOSMP010000000
VERSION     DAOSMP010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114627_length_1222_cov_23.756641"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PKX09_11155"
     CDS             <1..186
                     /locus_tag="PKX09_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOU18419.1"
                     /translation="EHGVLKTLAVTMLKNVPENHHSYPEAQRLLDMLSYFLPVDDTEI
                     PPTSIMREFIGKSSFTY"
     gene            complement(262..681)
                     /locus_tag="PKX09_11160"
     CDS             complement(262..681)
                     /locus_tag="PKX09_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOU18420.1"
                     /translation="MALSKGSVNKVILVGRLGRDPEVRYTPNGDAVAEVSLATTESVK
                     NRDGKREDRTEWHNLVIWRNQAEFAKEWLKKGQLVYVEGRLQTRQWEDKEGQKRSKTD
                     IQVDQITMLGGSGRGREKEAAEPETPPEPSPDDEIPF"
     gene            complement(783..>1222)
                     /locus_tag="PKX09_11165"
     CDS             complement(783..>1222)
                     /locus_tag="PKX09_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU18421.1"
                     /translation="CISGRREIVEFMRQRSRPYLFSNSVAPAVIGATLKALEIVQENP
                     DLPRRTIENAHHFRQKIEAAGFDIIPGNTAIIPIMIYDEPLAIKIADLLLEEGIYVIG
                     FTYPVVPRGTARIRVQVSAAHTPEQIDRAVAAFTKVRKSLNKG"
BASE COUNT          308 a          299 c          281 g          334 t
ORIGIN      
        1 gaacatggcg tcttgaaaac cttggcggtt accatgctga aaaatgtgcc ggaaaatcat
       61 catagctatc cggaagcaca acgcttattg gatatgctgt cgtattttct gccagtggac
      121 gatactgaaa ttccgccgac ctcgattatg cgggaattta tcggtaaaag ttcctttacc
      181 tattaatatg ctgagaccga ccggatcggt gcccaacggc gtcagattta tcctaagatc
      241 acaatgaatt ataagataat cttagaaggg aatttcatca tccggagatg gctcgggcgg
      301 cgtttctggt tcagcggctt ctttttcgcg tccgcgtccg gaaccaccta gcatagtaat
      361 ttggtctacc tgaatgtcag ttttactacg cttctgacct tctttatctt cccattgacg
      421 ggtttggaga cgaccttcga cgtagaccag ctgtcccttt ttgagccatt ccttggcaaa
      481 ttctgcctga ttgcgccaaa tcactagatt gtgccactcg gtgcggtctt cacgttttcc
      541 gtcacggttt tttaccgatt cagttgttgc taaggaaacc tcggcaactg catcgccgtt
      601 cggggtgtag cggacttccg gatcgcggcc aagacgacca accagtatga ccttgttaac
      661 acttcccttt gaaagagcca tcgttaatcc tcctttttgt taaaaattaa atcctcaaaa
      721 ttgggattgc aacttccgat gctaattcta caaaaaactt ataactttgt caaacactat
      781 ttttatccct tattgagaga tttccttact ttcgtaaaag ccgccacggc tcgatcaatc
      841 tgctcgggag tgtgagccgc cgaaacctga acgcgaatgc gggcggtgcc gcgcggtacc
      901 accggatagg tgaacccaat aacataaatg ccttcctcaa gcagaagatc ggcaatttta
      961 atggcgagag gttcatcgta aatcataatc gggataatgg cggtattgcc ggggataatg
     1021 tcgaaaccgg cggcttcgat tttctggcga aaatggtgtg cgttttcaat cgtgcggcgt
     1081 ggcaagtccg gattttcctg cacgatttcc aaagccttta aagtagctcc aataacggct
     1141 ggcgccacgg aattcgaaaa aagataggga cgcgaacgct gacgcataaa ttccacaatt
     1201 tcccgccgcc cggaaatgca ac
//
LOCUS       DAOSMP010000219         1222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_114630_length_1222_cov_15.476435, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000219 DAOSMP010000000
VERSION     DAOSMP010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114630_length_1222_cov_15.476435"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PKX09_11170"
     CDS             <1..659
                     /locus_tag="PKX09_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18422.1"
                     /translation="KKVYPFLRELGFIPILIGFIVVILIIRLISYRRKINPAVRMAGI
                     GFWVMGLESVAIILFQSLVGSVYLWIVFLTFAYMLGSGLGAIMQLKWKRADIILERVG
                     FLLMLILPFLLALPLTGGWPILLVTLWIGLILFIGGVVAGCIFPLMVKASTVQADKIA
                     NSAGLIYAADIFGSTLGTYLISGIVIPVYGIIPALGIVVAVELGIGAFNSPITHLTEK
                     "
     gene            complement(701..>1222)
                     /gene="amrA"
                     /locus_tag="PKX09_11175"
     CDS             complement(701..>1222)
                     /gene="amrA"
                     /locus_tag="PKX09_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOU18423.1"
                     /translation="LDLAEKTVAAAVNHRPLPDPKDVPERLKVKRGAFVTLEKHHELR
                     GCIGYILPIYPLYKTVIEMAKAAALDDPRFPPVSPSELKDITVEISVLTVPQKISDPS
                     EIEVGKHGIIIKRGFNQGLLLPQVATDYGWDRETFLEHTCLKAGLPRDAWQDKNTEIQ
                     IFSAQVFNRETVK"
BASE COUNT          282 a          237 c          324 g          379 t
ORIGIN      
        1 ttaaaaaagt atatccgttt ttgcgggaac tgggctttat cccgattctg attggtttta
       61 tagtagtgat tttaatcatt agattgattt catatcgaag aaaaataaac ccggcggtgc
      121 gaatggcggg gattggtttc tgggtcatgg gcttggaatc agttgccatt atcctgtttc
      181 agagtttagt tggtagtgtt tatttgtgga ttgtattttt gacttttgct tatatgctgg
      241 gttctggtct gggcgcaatt atgcaattaa aatggaagag agcagatatt attctcgagc
      301 gggttggctt tttactgatg ctaattttac cgtttctcct ggcgttgccg ctgaccggtg
      361 gatggccgat tttgctggtg acgctctgga tcggactaat tttatttatc ggtggagttg
      421 tggctggctg tatatttccg ttgatggtaa aagccagcac ggtacaagca gataaaatag
      481 ccaattctgc cggactgatc tatgccgccg atatttttgg ctcgacactc ggaacttatt
      541 tgatttccgg aattgtcatt ccagtatatg gaattatacc ggcgctggga attgtggtgg
      601 cggtagaatt gggaataggc gcattcaatt ctccgattac tcacctgacc gaaaaatagc
      661 tggataaaga ctgagtgata agtctctaat taattgtttg ttacttcacc gtctcacggt
      721 tgaatacctg agccgagaaa atttgaattt cggtattttt atcctgccag gcgtcgcgcg
      781 gtaagccggc tttcagacag gtatgttcga gaaaggtctc gcgatcccag ccgtaatccg
      841 tagctacctg cggtaagagc aagccttgat tgaagccacg tttaataatt atgccgtgtt
      901 tgccgacttc gatttccgat gggtcgctaa ttttttgcgg aacggtcagg acggaaatct
      961 ccacagtgat gtcttttaat tcactcggtg agacgggtgg aaagcgcgga tcgtccaggg
     1021 cggccgcttt agccatctcg attacggttt tatagagcgg gtaaatcggc agaatataac
     1081 caatgcaacc tcgcagttcg tgatgctttt cgagagtaac gaaagcgccg cgtttgactt
     1141 tcagacgttc cggaacgtct ttgggatccg gcagaggtct gtgattaaca gcggcggcga
     1201 cggttttctc agccagatcg ag
//
LOCUS       DAOSMP010000220         1198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_118110_length_1198_cov_7.708661, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000220 DAOSMP010000000
VERSION     DAOSMP010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118110_length_1198_cov_7.708661"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            467..>1198
                     /locus_tag="PKX09_11180"
     CDS             467..>1198
                     /locus_tag="PKX09_11180"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOU18424.1"
                     /translation="MTKKKLKIMLINPPNCGRSIPEEEYGITSLKQIFKGEPFNLEVL
                     AGPLIDHDVMIMDLKCEPDGALWKAFDDFQPDVVGLTSLTCEANTVIGIARGVKEKSN
                     AIVVIGGNHATYNPEYFNRADFDYIAVGLAKKSFRDLIDHLAFGREPTGLAGIARCAP
                     GAPLAYTKRSFDEKDLMDDIPPRYDLVAQYRDRYFLEHLGLTMGFVITAYGCTHRCSF
                     CTIPEMTGGKYLNHSAGAVLRDIQLL"
BASE COUNT          308 a          298 c          310 g          282 t
ORIGIN      
        1 atacggacga ggccaacgcg gcggaactgc cgccggtatc cgaaggttat ctggtacgca
       61 tcgagtcgcg caattagatg gaagcccccc aaaggactgg tacattggac ggcagcgcga
      121 ttccctgttt gccagagtgt tcaatttatg ataagcactt aaagctctcc ctgaaaagag
      181 agctttttta atgagaccca aatttcagaa acgtagtgag acttgctaaa atcgagcgaa
      241 gcgaagattg aagcataggt cgaactatcc cgcaaagcgg tggaagtgta ctgaaatttg
      301 atggtcgaat tatccccgag gcgaataacc taaaggtcac tataggccga gcgggataat
      361 tgtgggtcta attccccgac ccttggggcg caagtttctt tggatacttc gctgcttgcg
      421 gcggtgttgt ttaatccaga attttcgcgg caaacaaccg gctttgatga ctaaaaaaaa
      481 actgaaaatt atgctgatca atcctcccaa ctgcgggagg agcatccccg aagaagaata
      541 cggaatcaca tcgctgaaac agatttttaa gggagaacct tttaatctgg aagtgctggc
      601 cggtccgctg atcgatcatg acgtgatgat tatggatttg aaatgcgaac cggacggcgc
      661 cctctggaag gccttcgatg attttcagcc cgatgtagtc ggcctgacgt cgctcacctg
      721 cgaagccaat acggttatcg gcattgcccg cggagtaaaa gaaaaaagca atgccatcgt
      781 cgtgatcggc ggaaatcacg ccacctacaa cccggaatat tttaaccgtg ctgacttcga
      841 ttacattgcc gtcgggctgg ccaagaagag tttccgcgac ctgattgatc atctggcttt
      901 cggccgtgaa ccaacgggtc tggccggcat tgccagatgc gcgcccggcg cgccgctggc
      961 ctatacaaaa cgatcgttcg atgaaaagga tttaatggat gatatcccac cccgctacga
     1021 tctggtcgcc caatacagag atcgctattt cctggaacat ctcggtctga cgatgggttt
     1081 tgttattacc gcctacggct gcacgcaccg gtgttctttc tgcaccattc cggaaatgac
     1141 gggaggaaaa tatttgaacc actccgccgg ggccgtcctt cgcgatattc aattgctc
//
LOCUS       DAOSMP010000221         1197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_118247_length_1197_cov_19.649737, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000221 DAOSMP010000000
VERSION     DAOSMP010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118247_length_1197_cov_19.649737"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..>1197
                     /locus_tag="PKX09_11185"
     CDS             68..>1197
                     /locus_tag="PKX09_11185"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOU18425.1"
                     /translation="MKKLLLYCLTGFLLFQSCGFYGRQRGPTDWHRIPKPDYTIPPQA
                     QYIGGLKICLDPGHGGDNHIQNYKRGPTGLREAEINLRVALYLKEFLEKSGATVILTR
                     DGDYEIDLKKRTEMANFNQADIFISLHHNAATNPQTNYASTWYHGDADFAPASLDLAR
                     YIQQSLVENLRLPQQLPTGLLSDYLMHPNGFGVLQNLNMPGVLLEISFFTNPQEEKLL
                     SKPEYNRLEAYAIYLGIVRWAAAGIPKAILIEPRPDTTISNKQPTIYLKIKDGLHERP
                     KRWMLNREQYFSRQVTFFLDDSIAPFILDRDQNLIIHIPEKPLFNGWHTTYAEVVNYL
                     GNHNLPHRDRFRIAPPAQFLFAKAWNDRLPADGKAFTGIDVLA"
BASE COUNT          317 a          298 c          251 g          331 t
ORIGIN      
        1 ccaccgttgc ctttggttgt tttcatgtct aatttttgta agttttctga aaaaaagcga
       61 tttgttgatg aaaaaactct tactatactg ccttaccggc ttccttttat ttcagtcgtg
      121 cggattttat ggacgtcaac gcggtccgac cgactggcat cgcattccta aaccggacta
      181 taccattccg ccccaggctc aatacattgg gggcttgaaa atttgcctcg atccagggca
      241 tggcggtgat aatcatattc agaattataa gcgcggacca accgggttgc gcgaagccga
      301 gataaattta agagtggctt tatatctcaa agaattctta gaaaagtccg gcgcaacggt
      361 aattttaacc cgtgatggtg actatgaaat tgacttaaaa aaacgaactg agatggcgaa
      421 tttcaatcag gctgatattt ttatttcttt gcatcacaat gccgcaacca atcctcaaac
      481 caattatgct tcgacctggt atcacggcga cgccgatttt gcgccagcca gcctcgattt
      541 agcacgttat atccagcaaa gcttagttga aaatctgcgc ctgcctcaac agctaccgac
      601 cggtttgctc tcggattatt tgatgcaccc gaatggattc ggcgttctcc agaatcttaa
      661 tatgccgggc gttttactgg aaatttcctt cttcactaat ccccaagaag aaaaattgct
      721 cagcaaaccg gaatacaatc gcctcgaagc ttatgctatt tacctcggta tcgtgcgctg
      781 ggcggcggct ggcattccaa aagctattct gattgaaccc cgtcctgata ctacgattag
      841 caacaagcaa ccaacaattt atttgaaaat caaagacggc ttgcatgagc gccctaagcg
      901 ttggatgcta aaccgcgagc aatatttcag tcggcaggtg accttcttcc tcgacgattc
      961 gattgcacca tttattctgg accgcgatca aaacctgatt attcatatac cggaaaaacc
     1021 gctattcaac ggctggcata ccacttatgc cgaggtcgtc aattacctcg gcaatcataa
     1081 tttgccgcat cgagaccgct tccggatcgc accgccggct cagtttctat ttgcaaaagc
     1141 ctggaatgac cgtctgcctg ccgatggaaa agcttttact ggaattgatg tcttagc
//
LOCUS       DAOSMP010000222         1194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_118685_length_1194_cov_15.541703, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000222 DAOSMP010000000
VERSION     DAOSMP010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118685_length_1194_cov_15.541703"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..851)
                     /locus_tag="PKX09_11190"
     CDS             complement(75..851)
                     /locus_tag="PKX09_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU18426.1"
                     /translation="MAKIITVANQKGGVGKTTTAVNVAAGLAVSEIPTLLIDMDPQSN
                     ATIGCGIDYRNLENTVYEVLIEGFDINTAIMPSEIPYLDILPASTRLVGAEIEMVSLI
                     GRELVLKKSLELLTRDYKYVIIDCPPSLGLLTINSLAASDSVLIPIQCEYYAMEGLGQ
                     LLNTIRLVQRSLNRNLQIEGVLITMYDSRLRLSHEVSEQVRSHFKGKVYKTVVRRNVR
                     LGESPSFGKPIILYDATSAGAQDYMNLVGELLNDDKQTVR"
     gene            complement(995..>1194)
                     /locus_tag="PKX09_11195"
     CDS             complement(995..>1194)
                     /locus_tag="PKX09_11195"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOU18427.1"
                     /translation="ESALIAVATTLGGVGPGIGLFGPIESYAALSVLNKIVLMASMLF
                     GRLEFYSILIVFLPTFWQRAH"
BASE COUNT          353 a          279 c          234 g          328 t
ORIGIN      
        1 ccacttcgat ttcagccatc gtataaacct gttctattcc ttccggaaat tcagggatta
       61 gtgcgctaat acctttacct aaccgtttgc ttgtcgtcat tgagtagttc tcctaccaga
      121 ttcatatagt cctgagcgcc agccgaggta gcatcataaa gaatgatcgg tttaccaaag
      181 ctgggtgatt caccgaggcg gacattgcgc cggactacag ttttgtaaac ctttcctttg
      241 aaatgactgc gcacctgttc cgatacttca tgggaaaggc gcaggcgact gtcatacatc
      301 gtaatcagca cgccctcgat ttgtaagttg cggtttaaac tacgctggac caggcggatc
      361 gtatttagta attgcccgag cccctccatc gcataatatt cgcactgaat tggaattagc
      421 accgaatctg aagcggctag actattgatc gtcaagagtc ccagtgaagg tgggcaatca
      481 attattacat acttataatc acgggttagc aattcgagag atttctttaa aactaattct
      541 cggccgatta gtgaaaccat ctcaatttca gcgcctacta aacgggtgga agctggcaaa
      601 atatcaagat aaggaatttc ggaaggcatt atcgccgtgt ttatatcaaa gccttcgatt
      661 aatacctcat aaaccgtatt ttccaaattg cgataatcta tgccgcatcc gatcgtggcg
      721 ttcgattgcg gatccatatc aatcagcaaa gtaggaattt cggaaaccgc cagtccggcg
      781 gcaacgttaa ccgccgttgt ggtttttccg acaccacctt tttgattagc aaccgtgatt
      841 atcttggcca tctagctttt attaatttct ttaatttcaa tatatttcaa gtattaaaat
      901 attattaaaa aaccaagcaa atttaaatcc cgcctactct aaaaaacaag ttaaaagcct
      961 tatagcagag agtaagtctt cccacaacac tgacttaatg agcccgttgc cagaaagtag
     1021 gaaggaatac aattaaaatt gaataaaact ccaatcgccc aaatagcata ctagccatta
     1081 aaactatttt attcagaacg gacagagcgg cataactctc tatcggacca aataaaccga
     1141 tgcctggtcc aacgcctccc agggttgtag ccacggcaat gagcgctgat tccg
//
LOCUS       DAOSMP010000223         1193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_118833_length_1193_cov_13.065905, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000223 DAOSMP010000000
VERSION     DAOSMP010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118833_length_1193_cov_13.065905"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PKX09_11200"
     CDS             <1..200
                     /locus_tag="PKX09_11200"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOU18428.1"
                     /translation="ESALIAVATTLGGVGPGIGLFGPIESFAALSVLNKIVLMASMLF
                     GRLEFYSILIVFLPTFWQRAH"
     gene            343..1119
                     /locus_tag="PKX09_11205"
     CDS             343..1119
                     /locus_tag="PKX09_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU18429.1"
                     /translation="MAKIITVANQKGGVGKTTTAVNVAAGLAVSEIPTLLIDMDPQSN
                     ATIGCGIDYRNLENTVYEVLIEGFDINTAIMPSEIPYLDILPASTRLVGAEIEMVSLI
                     GRELVLKKSLELLTRDYKYVIIDCPPSLGLLTINSLAASDSVLIPIQCEYYAMEGLGQ
                     LLNTIRLVQRSLNRNLQIEGVLITMYDSRLRLSQEVSEQVRSHFKGKVYKTVVRRNVR
                     LGESPSFGKPIILYDATSAGAQDYMNLVGELLNDNKQTVR"
BASE COUNT          330 a          234 c          276 g          353 t
ORIGIN      
        1 cggaatcagc gctcattgcc gtggctacaa ccctgggagg cgttggacca ggcattggtt
       61 tatttggacc gatagaaagt tttgccgctc tgtccgttct gaataaaata gttttaatgg
      121 ctagtatgtt atttgggcga ttggaatttt attcaatttt aatcgtattc cttcctactt
      181 tctggcaacg ggctcattaa gtcagtgatc tgggaagact tactctccac taccaggctt
      241 ttaacttgtt ttttagagta ggcagggttt aaatttgctt ggttttttaa taatatttaa
      301 tacttgaaat atattgaaat taaagaaatt aatagaagct agatggccaa aataatcacg
      361 gttgctaatc aaaaaggtgg tgtcggaaaa accacaacgg cggttaacgt tgccgccgga
      421 ctggcggttt ccgaaattcc tactttgctg attgatatgg atccgcaatc gaacgccacg
      481 atcggatgcg gcatagatta tcgcaatttg gaaaatacgg tttatgaggt attaatcgaa
      541 ggctttgata taaacacggc gataatgcct tccgaaattc cttatcttga tattttgcca
      601 gcttccaccc gtttagtagg cgctgaaatt gagatggttt cactaatcgg ccgagaatta
      661 gttttaaaga aatctctcga attgctaacc cgtgattata agtatgtaat aattgattgc
      721 ccaccttcac tgggactctt gacgatcaat agtctagccg cttcagattc ggtgctaatt
      781 ccaattcagt gcgaatatta tgcgatggag gggctcgggc aattactaaa tacgatccgc
      841 ctggtccagc gtagtttaaa ccgcaactta cagatcgagg gcgtgctgat tacgatgtat
      901 gatagtcgct tacgtctctc ccaggaagtt tcggaacagg tgcgcagtca tttcaaagga
      961 aaggtttaca aaactgtggt ccggcgcaat gtccgcctcg gtgaatcccc cagctttggt
     1021 aagccgatta tcctttatga tgctacctcg gctggcgctc aggactatat gaatctagta
     1081 ggagaactac tcaatgacaa caagcaaacg gttaggtaaa ggtattagcg cattaatacc
     1141 tgaatttccg gaaggaatag aacaggttta tacgatggct gaaatcgaag tgg
//
LOCUS       DAOSMP010000224         1193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_118834_length_1193_cov_13.007030, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000224 DAOSMP010000000
VERSION     DAOSMP010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118834_length_1193_cov_13.007030"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..>1193
                     /locus_tag="PKX09_11210"
     CDS             7..>1193
                     /locus_tag="PKX09_11210"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU18430.1"
                     /translation="MQIKTLRHIPEIPADNRPLTHYKKIELTCDILVIGGGPAGLMAA
                     LELAQLNYNVIVVDNKEKLGGKLLLQTHKFFGSIEDCYAGRRGIEIAALLEKELSNHA
                     NIKIFTETTVAAIYKDKKAGLFIKNQNYAIVAFSGLIVSPGAHEKSLIFNGNHLPGVF
                     GAGAFQTLVNRDLVQAGRRIFIVGSGNVGLIAAYHALQAGITVVGICDILDKVSGYKV
                     HADKIERMGVPIYLKHTVIAAEGHTKVERVTIAVVDDNFQPFLETTQTFAVDTLLIAV
                     GLTPLDDFFEMANQFGFPVVKAGDADEIAEASSAMFSGRIAARRLANQLGKNIAIDST
                     FIQKAEILKNKPGRIFPSTPVVLDEKFQPIIRCTEEIPCNPCTSVCPVNAIQLRGDRH
                     DLM"
BASE COUNT          315 a          249 c          279 g          350 t
ORIGIN      
        1 ggcggtatgc agattaaaac cctgcgccat attccggaaa ttccagccga taaccgccca
       61 ctgacccact acaaaaaaat cgaattgact tgtgatatat tggtaattgg cggcggacct
      121 gccggattga tggccgctct cgagctcgcc caactgaatt ataacgttat cgttgttgac
      181 aataaggaaa aattaggcgg taaacttcta cttcagacgc ataaattttt cggctcgatt
      241 gaagactgtt atgccggtag gcggggcatt gaaattgccg ctttactgga gaaagaatta
      301 agcaatcacg ccaatattaa aatttttact gagactactg tagccgcaat ttacaaggat
      361 aaaaaagccg ggctttttat taaaaaccaa aactatgcga ttgttgcctt tagcggtttg
      421 atagtttctc ccggtgctca cgaaaagtca ttgattttca atggcaatca ccttccagga
      481 gttttcggtg ctggtgcttt tcagaccttg gtcaatcgtg atctggttca agctggtcgt
      541 cgcattttca ttgttggtag cggcaatgtc ggtttgattg cggcttatca tgctttgcaa
      601 gccggaatta cagttgtcgg catttgtgac attcttgata aagtttctgg ctataaggtt
      661 catgccgaca aaatcgagcg gatgggcgtg ccgatctacc tgaaacacac ggtcatcgcc
      721 gccgaaggtc acaccaaagt cgagagagtt accattgcgg tagttgatga taattttcaa
      781 ccttttttgg aaacgaccca aacttttgca gttgatactt tactaatcgc ggttggttta
      841 actccacttg atgatttttt cgagatggct aaccaatttg gttttccggt tgttaaagcc
      901 ggtgatgccg atgaaatcgc cgaggcttca tcggcgatgt ttagcgggcg aattgcggca
      961 cggcgtctgg caaatcagct tggtaaaaat atagcaatag attcgacttt tatccagaag
     1021 gctgaaattt taaaaaacaa gccgggacga atttttccat caactccggt agttttggat
     1081 gaaaaatttc agccgattat ccgttgtacg gaagaaattc cgtgcaatcc ttgtacttcg
     1141 gtttgcccgg tgaatgccat tcaacttcgc ggtgatcgtc acgatttaat gga
//
LOCUS       DAOSMP010000225         1193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_118871_length_1193_cov_2.831283, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000225 DAOSMP010000000
VERSION     DAOSMP010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118871_length_1193_cov_2.831283"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1193
                     /locus_tag="PKX09_11215"
     CDS             <1..>1193
                     /locus_tag="PKX09_11215"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOU18431.1"
                     /translation="QGNTDWSESAGTSVENSEWIVYNDQTFSYLGAHVYEGGANLPPA
                     ARAGVDQVVRFNDTVTLDGSASNDQDGSIASYAWAQISGESVTLSATDQAIVTFISPS
                     SVATLVFELVVTDNESAVGKDSVTIYVDPSPVIISEYVEGTSNNKYIELCNTSDATID
                     LSANGYTLRLASNGGGSFTNTFSDWGTFSQLASGGVIVLGKISTGIYPNVNITDENVI
                     NFNGNDAIGLFRNGLLVDVVGNPNLSDSIIIDVTLRRKNTVLYGSSTYIPSEWDQYET
                     DNVANLGSHSTNPNAPIVTNIAYLPDFITSADEITVSATITPVVGSIDSAKIYYGAPG
                     SLINIAEMWKETGDTWMGNIPSQTGNSIIEFYIYCQDDQSPTNSGKSATQTLIIANST
                     PETIA"
BASE COUNT          336 a          244 c          280 g          333 t
ORIGIN      
        1 acaggggaat acagactggt ctgaatcagc cggaacaagt gtcgagaatt cagaatggat
       61 tgtctataat gatcaaactt tttcatattt gggtgctcat gtttatgaag gcggggcaaa
      121 ccttccgccg gctgccagag ccggcgtcga ccaggtagtg cgttttaacg acacagtcac
      181 actggatggt tcagccagta atgaccaaga tggttcgatt gccagctatg cctgggcgca
      241 gatttcggga gaatcggtga ctctgtcggc taccgatcag gctattgtaa cctttatttc
      301 accgtccagc gtagctaccc tggtattcga attggtcgtt acggataatg aaagcgccgt
      361 cggaaaagac agcgtgacga tttatgttga tcccagtcct gttataatca gtgaatatgt
      421 tgaagggact tcgaataata aatatattga attatgtaat acatcagacg ctactataga
      481 tttgagtgcc aatggttata cattacgttt agcttcgaat ggcggtggtt catttaccaa
      541 tactttttca gattggggta catttagtca gttagctagt ggtggtgtga tagtgctagg
      601 aaaaattagt actggcattt atccaaacgt aaatatcacc gatgaaaacg tcattaattt
      661 caatggcaat gacgcaattg gcttatttcg taatggttta ttagttgatg tagttggaaa
      721 tccgaatctt tctgatagta ttattattga cgtaacacta agacgtaaga atacggttct
      781 gtacggcagt tcgacctata taccgagcga atgggatcaa tatgagaccg acaatgtcgc
      841 taatctcggt tcgcacagta ctaatccgaa tgccccgatc gtcactaata tcgcatattt
      901 acccgatttt atcacttcgg ctgacgagat taccgtcagc gcaacgatta cgccggtggt
      961 agggagtatc gattcggcga agatttatta cggggcaccc ggtagtttga ttaacatcgc
     1021 cgagatgtgg aaggaaaccg gtgatacatg gatgggcaat ataccgtccc agaccggcaa
     1081 tagcattatc gaattttata tttattgcca agacgatcag tctccaacca attccggtaa
     1141 atcagctacc caaacgctga taatcgccaa tagcactccc gagacaatcg cca
//
LOCUS       DAOSMP010000226         1183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_120354_length_1183_cov_10.958333, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000226 DAOSMP010000000
VERSION     DAOSMP010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120354_length_1183_cov_10.958333"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..>1183
                     /locus_tag="PKX09_11220"
     CDS             304..>1183
                     /locus_tag="PKX09_11220"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOU18432.1"
                     /translation="MFGKGIRRSFIGLSVLLVAMAYGDSNIVINPGFEDGKEGWFDRT
                     CAIEIVTSPVYEGRYSGKAFQRLANWQGIKQSVFGRMVDGKTYQVSGWVRLDNAPSDE
                     VAISFEQQDDSGTKYIGVVRGIATDTAWTKLSGEFTLNVTGTLSVLDVYFEGPAPGVN
                     FFVDDVVVYGPEANPPVIIPPTPQSKSQVDAKIRHQIIEGFGASGAYYTMNLVNHNKK
                     TELYNLLFKELGLDIFRIRNNYDMEPHSMQETVEIIKNAKALRGENLKILMSSWSPPA
                     YLKSNNNVVGGTLKKVA"
BASE COUNT          348 a          232 c          271 g          332 t
ORIGIN      
        1 tgactgcctt cactttgcta cagttcctga attctatcaa taataaaccc ctaaaccata
       61 ttaaacatgc gctcgtttaa ttccacacaa caggttttcc ttattcatta ttaacgttaa
      121 atatttcaaa tgatagagca ttgaccttct aagcaggtga ttacaggttc tttcacagga
      181 aacccgtcca ctcggagatt tttatccagt tagttttgat tactgaaaat aagtttttaa
      241 aattggcaat aaattgtaga tttttatccg ttatgaaatt ctaaattaag gagtattagc
      301 aaaatgtttg ggaaaggaat tcgcagatca tttatcggat tgagcgttct gctggtagca
      361 atggcttatg gggatagtaa cattgtaatt aatcccgggt ttgaagatgg aaaagaaggt
      421 tggtttgatc gcacttgcgc catagaaatt gtaacctcgc cggtatatga aggtcgctat
      481 agcgggaaag cttttcagcg cctggctaac tggcagggta tcaagcagtc ggtatttggc
      541 aggatggttg acggcaaaac ctatcaagtt tccggctggg tcagattaga taatgcgccc
      601 agcgacgaag tcgccatttc ttttgaacaa caagacgaca gcggaacaaa atatatcggg
      661 gtcgttcgcg ggatagccac cgataccgcc tggactaaac tttccggtga attcacgtta
      721 aatgtgactg gtactttatc ggttctggat gtgtatttcg aaggaccggc gccgggcgtc
      781 aatttcttcg tggatgatgt agtcgtatat ggtccagagg cgaatccacc ggtaatcatt
      841 ccacccacgc cgcaaagcaa aagccaggta gatgccaaaa tccgtcacca aataatagaa
      901 ggcttcggcg cttctggtgc atattacacg atgaatctgg taaatcataa taaaaagacg
      961 gaattgtata acctgctttt caaagaactc gggctggaca tattccgcat tcgcaataat
     1021 tatgatatgg aaccgcattc gatgcaggaa accgtcgaga ttatcaaaaa cgctaaggca
     1081 ctgcggggag aaaatttgaa aattttgatg tcttcgtggt ctcctccggc ttatttgaag
     1141 agtaataaca atgtggtagg tggaacgctc aaaaaggtcg ccg
//
LOCUS       DAOSMP010000227         1157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_124520_length_1157_cov_9.765880, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000227 DAOSMP010000000
VERSION     DAOSMP010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124520_length_1157_cov_9.765880"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="PKX09_11225"
     CDS             complement(<1..1151)
                     /locus_tag="PKX09_11225"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOU18433.1"
                     /translation="MQIKTLRHIPEIPADNHPLTHYQKIELTCDILVIGGGPAGLTAA
                     LELAQLNYNVIVVEDKEKLGGKLLLQTHKFFGSIEDCYAGRRGIAIAALLEKELSNHT
                     NIKIFTNTTVAAIYKDKKAGLFIKNQNYAIVAFRGLIISAGAREKSLIFNGNYLPGVF
                     GAGAFQTLVNRDLVQAGRRVFIVGSGNVGLIAAYHALQAGITVVAICDILGKVSGYKV
                     HADKIKRMGVPIYLKHTVIAAEGNGKVERVIIAAVDDHFQPLLETARTFAVDTLLIAV
                     GLTPLDDFFEMANQFGFPVVKAGDADEIAEASSAMFGGRIAARRLANQLGKNIAIDST
                     FIQKAEILKSKPGRIFPSTPVVLDEKFQPIIRCTEEIPCNPCTAVCPVNA"
BASE COUNT          334 a          267 c          246 g          310 t
ORIGIN      
        1 gcattcaccg ggcaaaccgc cgtgcaaggg ttacagggaa tttcttccgt acaacggata
       61 attggttgaa atttttcatc caaaactact ggagttgatg gaaaaattcg tccgggcttg
      121 ctttttaaaa tttcagcctt ttggataaaa gtcgaatcta ttgctatatt tttaccaagc
      181 tgatttgcta aacgccgtgc cgcaattcgc ccaccgaaca tagccgatga agcttcggcg
      241 atttcatcgg catcaccggc tttaacaacc ggaaaaccaa attggttagc catctcgaaa
      301 aaatcatcaa gtggagttaa accaaccgcg attagtaaag tatcaactgc aaaagttcga
      361 gcggtttcca ataaaggttg aaaatgatcg tcaaccgcgg caatgataac tctctcgact
      421 ttgccgttgc cttcggcggc gattacggtg tgttttaagt agattggcac acccatacgt
      481 tttattttat cggcatgaac cttatagccg gaaaccttac ccagaatatc acaaatggcg
      541 acaactgtga tcccagcttg taaagcatga taagccgcaa tcaaaccgac attgccacta
      601 ccaacaatga aaacgcgacg accggcttga accagatcac gattgaccag ggtctgaaaa
      661 gcaccggcgc caaaaactcc cggcaggtaa ttgccattga aaatcagtga cttttcgcga
      721 gcaccagcag aaattatcaa accgcgaaag gcaacaatcg catagttttg gtttttaata
      781 aaaagcccgg cttttttatc cttgtaaatt gcggctacag tagtattagt aaaaatttta
      841 atattggtgt gattgcttaa ttctttctcc agtaaagcgg caattgcgat gccccgccta
      901 ccggcataac agtcttcgat cgatccgaaa aatttatgcg tctgaagtag aagcttaccg
      961 cctaattttt ccttatcttc aacaacgatt acgttataat tcagttgggc aagctcgaga
     1021 gcggccgtca atccggcagg tccaccgcca attaccaata tatcgcaggt caattcgatt
     1081 ttttggtagt gggtcagtgg gtggttatcg gctggaattt ccggaatatg gcgcagggtt
     1141 ttaatctgca taccgcc
//
LOCUS       DAOSMP010000228         1152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_125353_length_1152_cov_14.456700, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000228 DAOSMP010000000
VERSION     DAOSMP010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125353_length_1152_cov_14.456700"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PKX09_11230"
     CDS             complement(<1..1007)
                     /locus_tag="PKX09_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18434.1"
                     /translation="MFKKGLILLILASAVFAQSNPPAIRPDSVGIYFNMSIQAYSEKQ
                     YDRYLEYTQKLIQVYPDDFTLQYNLASAYALNKDTVNAIKILNSLVDKGLGLVAEDDP
                     DFADLKNTPEFRALVKKIEKVKKPVKSSKKAFTVAEKDLIPRGITYDSQTKNLYISSL
                     YKSKIVQIDRNGKISDFVPERQDGLGPVTAIQADPQRRILWALSSFGIPNEKTPRELA
                     GTASVYKYNLNDGKLLKKYDLPRPNRHFLTDLTEDRAGNLYIADSGQRTIFRLDAQKD
                     TLEKFLELYKYHQLTSLTITPDDKYLFVTYGGGILNIEIATSKITPLTSPDHILLVGC
                     DG"
BASE COUNT          300 a          230 c          271 g          351 t
ORIGIN      
        1 ccgtcacaac ctaccagcaa aatatggtcc ggactggtta gcggtgtgat tttgctggtg
       61 gcaatttcga tgttcagaat accgccgcca tacgttacga atagatattt atcatcgggt
      121 gtaatggtca agctggtcaa ttggtgatac ttatacaatt cgaggaattt ttcgagagta
      181 tccttttggg catcgaggcg gaaaatagtc cgctggccgg aatcggcaat ataaagattg
      241 ccagcccggt cttctgtcaa atcagtcaga aaatgccgat taggtcttgg caaatcgtat
      301 ttcttaagga gtttgccgtc atttaggttg tatttgtaaa cgctggcggt tccagccaat
      361 tcccggggcg ttttttcatt ggggattcca aatgaactta gcgcccacaa aatccgacgc
      421 tggggatcag cctgaatggc ggttaccgga ccaagtccat cctgccgttc aggaacaaaa
      481 tcgctaattt taccattgcg gtcaatttgg acaattttcg atttataaag actgcttata
      541 tataggtttt tagtttgtga gtcgtaggtg atgccgcgcg gaatgaggtc tttttcggca
      601 acggtaaagg cttttttact cgattttacc ggctttttaa ccttttcaat tttcttgacc
      661 agagcgcgga attccggtgt atttttcaaa tcggcaaaat cgggatcgtc ttctgctacc
      721 aaccctaagc ctttatctac taatgaattc aggattttga tagcattgac agtgtcctta
      781 tttaaagcat aggcactggc gagattatac tgtagggtaa aatcgtctgg ataaacctga
      841 ataagcttct gagtatattc gagatagcga tcatattgct tttcactgta agcttgaata
      901 ctcatattga agtaaatccc gacgctatcc ggtcgaatcg cgggtggatt ggattgagca
      961 aaaactgcgg aagctagaat taacaagatt aaaccttttt taaacataat taattacttt
     1021 cctttattta aaataactac ttggcaagcg gatagttggg caaatttttc ggcatcagcg
     1081 tgactaccgg agacggcacc ccagtgcatc ggtacggcga cctgcgggtg gattattttg
     1141 gtggcttggg cg
//
LOCUS       DAOSMP010000229         1144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_126722_length_1144_cov_14.640955, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000229 DAOSMP010000000
VERSION     DAOSMP010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126722_length_1144_cov_14.640955"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PKX09_11235"
     CDS             complement(<1..680)
                     /locus_tag="PKX09_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18435.1"
                     /translation="MLISLNKKIILIGTILAFLPLSVLATNYYVRKSGNDSNNGLTPA
                     TAWKTVTKAANYNLQPGDTVFIGTGIYNEQLIPSRSGSSTAYIVYYGDYKGLKTQDPA
                     GRVTIGGLAAGSPVFVPQSASQNNFTFTLANIINNGDGTSTIKVGIRNNNRSNLNNTA
                     ISLPSGVTALYPANGSTWTSPKNNRYTINNPTSNPFYCIRYNLSSGSVYRGGSDTLIY
                     KLTTQQAEA"
     gene            complement(854..>1144)
                     /locus_tag="PKX09_11240"
     CDS             complement(854..>1144)
                     /locus_tag="PKX09_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18436.1"
                     /translation="EQMSPTEIQQAATNSGADLILLASVSSDQPNRVQDGFYFAHARG
                     VLKVYNISQQAFVGNYLVEDKNAGNSPENAGAKAIKKVSDALIQKFSDEFGL"
BASE COUNT          311 a          239 c          251 g          343 t
ORIGIN      
        1 gcttcggctt gctgagtagt aagtttatag attaatgtat cagagccacc tctataaaca
       61 gatccactcg aaagattgta tctaatgcag tagaatggat tactggttgg attattaatg
      121 gtgtacctgt tatttttggg actggtccag gtcgagccgt tggctgggta tagcgcggtg
      181 actcctgaag gtaaactgat cgctgtattg tttagattgg atctattgtt attgcggatt
      241 ccgaccttga ttgtgctggt tccatcgccg ttattgatga tgtttgccag ggtgaaggtg
      301 aaattattct gagaagcaga ctggggcacg aataccgggc taccagcggc aagacctccg
      361 atggttaccc gccccgccgg atcctgagtt tttaatcctt tgtaatcacc ataataaaca
      421 atataagcag ttgacgagcc ggaacgcgag ggtattaact gctcgttgta aattcccgtt
      481 ccgatgaaga ccgtatcgcc gggttgcaaa ttataattag cggctttagt gactgttttc
      541 caggcggttg ccggagtcaa accattattg ctgtcattgc cgcttttccg gacataatag
      601 ttcgtagcca aaaccgaaag cggtagaaat gccagtatcg ttccaataag gataattttt
      661 ttattaaggg aaatcaacat gaatttcctc cgccaattaa aatcttttta agaacataaa
      721 ttattgaagt agtgtttgtg accattgtca taaaaatcaa atgcttaaaa tacctactct
      781 acagtccatt attagttctt tctaaccatt tcaataaaaa gccacctgaa ttataccaca
      841 aaacaaattt caatcaaagc ccaaactcat cgctgaattt ttgtataaga gcatcgctaa
      901 cctttttgat agctttggct ccggcgtttt ctggtgaatt cccggcgttt ttatcctcaa
      961 ccagataatt gccgacaaac gcctgttggg aaatgttata aactttcaaa acgccgcggg
     1021 cgtgcgcgaa gtaaaatccg tcctgaaccc gattaggttg atccgaagaa acgctcgcca
     1081 gtagaatcaa atcagccccg ctattggtgg cggcttgctg aatttcggtt gggctcattt
     1141 gttc
//
LOCUS       DAOSMP010000230         1128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_129458_length_1128_cov_13.597390, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000230 DAOSMP010000000
VERSION     DAOSMP010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129458_length_1128_cov_13.597390"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..>1128
                     /locus_tag="PKX09_11245"
     CDS             60..>1128
                     /locus_tag="PKX09_11245"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOU18437.1"
                     /translation="MFLFLITVQLLQFRGKPTTKLQKLIVYGGAFIGAMTFAFTDSHW
                     FNAVEAEVYAFSTFLTAIVVWLILVWEEKAGQPGHERYLLIIAYIVGLAIGVHLLNLL
                     AIFFIALIIYFKQKKVSSVWWLILDIIIGVAVAGLLFLISMQFRVSIFVQAIITLGIL
                     VLGYYQLYRKARKPRLKEHAQNVLMAFAASAAFLIIYVGIMKGLPILADKVGLWATVL
                     AIVLLFAVTIWAISQKRNILALIMMSLVLIVIGYSTYVTIFIRSAQDPNIDENDPETT
                     HQAVAYLEREQYGQRSFTDIFNRRVWKPEAAHKYSSALDYFWNYQIKYMYIRYFNWQF
                     IGRKGSDVDFFQFILPFPFIIG"
BASE COUNT          276 a          229 c          243 g          380 t
ORIGIN      
        1 gccaggatat tgcgttccgg gttaatttaa tatccaccat cgtcagtgca ttggcggtga
       61 tgtttctctt tttaattact gttcagcttc tgcaatttcg cgggaaaccc acgaccaaat
      121 tacaaaaatt gattgtctat ggtggagctt ttattggcgc tatgactttc gctttcactg
      181 atagccactg gttcaacgcg gtggaagccg aagtctatgc cttttcgacg tttttaactg
      241 ctattgtggt ttggttgatt ttggtttggg aagaaaaagc cggtcagccc ggacatgagc
      301 gttatctatt aatcatcgcc tatattgtag gtctggcaat cggtgtccat ctcttaaatc
      361 tcctggcaat tttctttatc gccttgataa tttattttaa acaaaagaaa gtgtcttcgg
      421 tctggtggtt gattctggat ataattatcg gggtagcggt tgccggttta cttttcctta
      481 tttccatgca attcagagtg tcaatatttg ttcaggcgat aataacatta ggtattctgg
      541 tgctgggtta ttaccagctt tatcggaagg ctcggaaacc acgcctgaaa gaacatgctc
      601 aaaatgtctt gatggctttt gcggcttccg ccgcctttct gataatttat gtcggcataa
      661 tgaagggttt accaatcctc gccgataaag ttggactctg ggcaaccgtt ctggcaattg
      721 ttctcctgtt tgcggtaacc atttgggcaa ttagtcaaaa acgcaatatt ttagccttga
      781 taatgatgtc gctagtcctg atcgttatcg gatattctac ctatgttacc atttttatcc
      841 gcagcgctca ggatccaaat attgacgaaa atgatcccga aactactcac caggcggtcg
      901 cttacttaga gcgtgaacaa tatggtcagc gtagttttac cgatatattt aatcgccgag
      961 tttggaagcc agaagccgct cataaatatt ccagcgcttt ggactatttc tggaattatc
     1021 agattaaata tatgtatatc cgctatttca actggcaatt catcggacgt aaaggaagcg
     1081 atgtagattt ctttcaattt atcttgccat ttccgtttat tatcgggc
//
LOCUS       DAOSMP010000231         1099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_134765_length_1099_cov_20.416667, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000231 DAOSMP010000000
VERSION     DAOSMP010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134765_length_1099_cov_20.416667"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PKX09_11250"
     CDS             complement(<1..557)
                     /locus_tag="PKX09_11250"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOU18438.1"
                     /translation="MENQKKAKRIINTWTGPWEKRNLPKMAAALPNWVSSDHLTILGI
                     VAALVISLGYVLTRYSNWWLMLANFGFVLHWWADSLDGTLARVRHKEREQYGYFVDHM
                     CDAFSTTIILVAFGLSPLMRLDISLFLTIGYLLLNIYAHVGAYTEGIFRLSYAKVGPT
                     EIRILVVIVNTILIFWNPTVLVLNN"
     gene            complement(572..988)
                     /locus_tag="PKX09_11255"
     CDS             complement(572..988)
                     /locus_tag="PKX09_11255"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOU18439.1"
                     /translation="MNFKKIIKFQLVAWLGTIVNLGSLWILHGILKIPVTIAGACAIE
                     LAILHNFTWYYFRTWNGRVAHTPTDFFSRLWKYNVITAGVDFVFNLGTLSVLTHFFHI
                     HYILADILGMSIGPFIKIFINELVIFKKPKLHVKDN"
BASE COUNT          382 a          224 c          188 g          305 t
ORIGIN      
        1 tgattattta acaccagcac ggtaggattc cagaaaatta gaattgtatt tactattacc
       61 accagaattc gtatctcagt cggaccgacc ttggcatatg ataagcgaaa aataccttcg
      121 gtataagcgc ctacatgagc ataaatattt agcaataaat aaccgatcgt gagaaacaga
      181 ctaatgtcta aacgcatcag cggtgaaagt ccaaacgcaa ctagaataat cgtcgtcgaa
      241 aaagcgtcgc acatatgatc aacgaaatac ccgtattgtt cccgctcttt atggcgcacc
      301 cgcgccaatg ttccatcgag gctgtctgcc caccaatgta aaacaaagcc aaagtttgcc
      361 aacattaacc accaattcga atatctggtt agcacatatc caagcgaaat tactaaagcc
      421 gcgacgatac ctaaaattgt aagatggtcg gaagaaaccc agttcggtag agcggcagcc
      481 atcttaggaa ggttacgttt ttcccaggga ccagtccagg tattaatgat tcgtttggct
      541 ttcttttgat tttccattat gtctttttcc cctaattatc tttcacatgc aatttaggct
      601 tcttaaaaat gaccaattca ttaataaata ttttaataaa gggaccaatc gacattccca
      661 aaatatcagc taaaatataa tgaatatgaa aaaaatgggt aagtaccgat aatgtaccca
      721 aattgaaaac aaaatccact ccggctgtaa taacattgta tttccagagt cttgagaaaa
      781 aatcagtggg agtgtgggca actctaccat tccaagtgcg gaaataatac caggtaaaat
      841 tatgcaaaat agctaattca attgcgcagg ctccggcaat cgtcaccggg atttttaata
      901 taccgtgcaa tatccaaagt gaaccaagat taacaatcgt tcccagccag gctacgagtt
      961 gaaatttgat aattttcttg aaattcattt aattagtgac gttataaatc aaatagtcaa
     1021 aatgctcata caggcgcaaa tataaaatat ttattatagt tttcaaactc taatatcttt
     1081 gctggaaatc cttaataaa
//
LOCUS       DAOSMP010000232         1061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_142372_length_1061_cov_13.346918, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000232 DAOSMP010000000
VERSION     DAOSMP010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142372_length_1061_cov_13.346918"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /gene="selB"
                     /locus_tag="PKX09_11260"
     CDS             complement(<1..752)
                     /gene="selB"
                     /locus_tag="PKX09_11260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOU18440.1"
                     /translation="MQSLVIGTAGHIDHGKSALVKALTGTDPDRLAEEHLRGMTIDIG
                     FAFLTENIAFIDVPGHERFIKNMVTGASTIDVAMLVIAADDGIMPQTREHFEILKLLG
                     IQHGIIVVNKIDLVKPDWLEMVVEDVRQLVEGSFLENAPLFKTSTVTGSGIAELCRFL
                     IELPNQIPEVRSTSLFRLPVDRVFSVKGFGTVVTGTVLSGEIKVGEEVEIMPSGQRAK
                     IRSLQSHNQPLDSITRGQRAALNLVGVSKEQI"
     gene            complement(764..>1061)
                     /locus_tag="PKX09_11265"
     CDS             complement(764..>1061)
                     /locus_tag="PKX09_11265"
                     /inference="COORDINATES: protein motif:HMM:NF015780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18441.1"
                     /translation="LNAIEPEIIKTLNLTVREIPTEAGSGAMPTAQIPSVAIVAIPQR
                     ISETQLAQKFRSYTPPIVGYCRNGEFRLDLKAVQSDDLPLIQSALQAIGRSLLN"
BASE COUNT          280 a          255 c          255 g          271 t
ORIGIN      
        1 tcgatttgtt ctttacttac tccaaccaga ttaagggccg cacgttgacc gcgggtaatg
       61 gaatcaagag gttgattatg cgattgcaga ctgcgaattt tcgcgcgctg accggacggc
      121 ataatctcaa cctcttctcc aactttaatt tcaccggaaa gcaccgtccc tgtgacaact
      181 gtgccgaagc cttttaccga aaaaacgcga tctaccggca ggcgaaataa actggtggaa
      241 cgcacttcgg ggatttggtt gggcaattca atcaaaaacc ggcacaactc cgcaattccg
      301 ctaccggtaa ctgtcgaagt tttaaaaagc ggcgcatttt ccagaaaaga accttcgact
      361 aattgtcgaa catcctctac gaccatttcc agccagtcgg gcttcacaag gtcaatcttg
      421 tttacgacaa taattccgtg ctgaattccc agcagtttga gaatttcaaa atgctcgcgg
      481 gtttggggca ttatgccatc atcggcggca attacaagca tagcgacatc aatggtactg
      541 gcgccagtca ccatattttt gataaaacgt tcatggccgg gcacatcaat gaacgcaata
      601 ttttcagtta aaaaggcgaa gccgatgtca atggtcatac cgcgcaggtg ttcttctgcc
      661 agacgatcag ggtcggtacc ggtaagggct ttgactagcg ccgatttccc atgatcaata
      721 tgtccggcgg tgccgataac gagagattgc attttcttta taactaattt agtaaggaac
      781 gaccaatcgc ctgtaaggca gattgaataa gcggtaaatc atcagattga acggctttca
      841 aatccaggcg aaattctccg ttccggcagt agccgacaat cggcggtgtg taactgcgaa
      901 atttttgcgc caattgggtc tcggaaatac gctgagggat tgccacaatg gcaacgctgg
      961 gaatttgggc ggttggcata gcgccgctcc cagcctcggt cggtatttca cgcaccgtta
     1021 ggttgagcgt tttaataatt tccggctcaa tagcatttag g
//
LOCUS       DAOSMP010000233         1059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_142796_length_1059_cov_10.962151, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000233 DAOSMP010000000
VERSION     DAOSMP010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142796_length_1059_cov_10.962151"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PKX09_11270"
     CDS             <1..298
                     /locus_tag="PKX09_11270"
                     /inference="COORDINATES: protein motif:HMM:NF015780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18442.1"
                     /translation="LNAIEPEIIKTLNLTVREVPTEAGSGALPTAQIPSVAIVAIPRY
                     ISETQLAQTFRSYSPPIVGYCRNGEFRLDLKAVQPDDLPIIQSALQAIGRSLLS"
     gene            308..>1059
                     /gene="selB"
                     /locus_tag="PKX09_11275"
     CDS             308..>1059
                     /gene="selB"
                     /locus_tag="PKX09_11275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOU18443.1"
                     /translation="MQALIIGTAGHIDHGKSALVKALTGTDPDRLAEEQERGMTIDIG
                     FAFLNENIAFIDVPGHERFIKNMVTGASTIDVAMLVIAADDGVMPQTREHFEILKLLG
                     IQHGIIVVNKIDLVQPDWLEMVVEDVRQLVEGSFLENAPLFKTSTVTGSGIAELRRFL
                     IELPNQIPEVRSTGLFRLPVDRVFAIKGFGTVVTGTVLSSEIKVGEEIEIMPSGKRAK
                     IRSLQSHNQPLDSVARGQRAALNLVGVSKEQI"
BASE COUNT          265 a          253 c          265 g          276 t
ORIGIN      
        1 cctaaatgct attgagccgg aaattattaa aacgctcaac ctaacggtgc gtgaagtacc
       61 gactgaagct gggagcggcg ctctgccgac cgcccaaatt cccagcgttg ccatcgtggc
      121 aattccccgt tatatttccg agacccaatt agcccaaaca tttcgcagtt actcaccgcc
      181 gatcgtcggt tattgccgga acggagaatt tcgcctggat ttgaaagccg ttcaacctga
      241 tgatttgcca attattcaat cggctttgca ggcgatcggt cgttccttac taagttaatt
      301 ataaaaaatg caagcactca ttatcggcac cgccggacat attgatcacg ggaaatcggc
      361 gctggtcaaa gcccttactg gcactgatcc cgatcgtctg gccgaagaac aggaacgcgg
      421 tatgaccatt gacatcggct ttgccttttt aaatgaaaat attgcgttta ttgatgtgcc
      481 ggggcatgaa cgctttatta aaaatatggt gaccggcgcc agtaccattg atgtcgctat
      541 gcttgtaatt gccgctgatg acggcgtgat gccacagacg cgcgagcatt ttgaaattct
      601 caaactgctg ggaattcagc acgggattat cgtcgtgaac aagattgacc tcgttcagcc
      661 cgactggctg gaaatggtcg tagaggatgt tcgacaatta gtcgaaggtt cttttctgga
      721 aaatgcgccg cttttcaaaa cttcgacagt taccggtagc ggaattgcgg aattgcgccg
      781 gtttttgatt gaattgccca accagattcc cgaagtgcga tccaccggtt tgtttcgcct
      841 gccggtagat cgcgtttttg cgatcaaagg cttcggcaca gttgtcacgg ggacggtgct
      901 ttctagtgaa attaaagttg gcgaagaaat tgagattatg ccgtccggaa agcgcgcgaa
      961 aattcgcagt ctgcaatcgc ataatcaacc tcttgattcc gttgcccgcg gtcaacgtgc
     1021 ggcccttaat ctggttggag taagtaaaga acaaatcga
//
LOCUS       DAOSMP010000234         1053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2394
            NODE_144082_length_1053_cov_7.086172, whole genome shotgun
            sequence.
ACCESSION   DAOSMP010000234 DAOSMP010000000
VERSION     DAOSMP010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864461
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,257
            CDSs (total)                      :: 2,208
            Genes (coding)                    :: 2,196
            CDSs (with protein)               :: 2,196
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144082_length_1053_cov_7.086172"
                     /isolate="AMR_MDS_2394"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="PKX09_11280"
     CDS             <1..725
                     /locus_tag="PKX09_11280"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOU18444.1"
                     /translation="AIYSTFLQRAYDNIIHDVALQNLPVVFVLDRGGIVGEDGPTHHG
                     AFDLSYLCTVPNMIVAAPRNGTELRNLMFTALEQNDAPFAIRYPKDSYVEFDDQAPAV
                     LYQIGKWEIIHNGQDVAILSVGVMTNNAIEAACLLKSKNIFPTIVHARFVKPLDTQML
                     SEIASKHRIIITIEENSIVGGFGEQVARYFAEQNLPQKINILAIPDHFIEQGAREILL
                     RDLALDAIGIANKVEQIYRQAD"
     gene            861..>1053
                     /locus_tag="PKX09_11285"
     CDS             861..>1053
                     /locus_tag="PKX09_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU18445.1"
                     /translation="MQKFKIIIIAGVAVLFSFCDLKIPSESDFPTWSVSLSIPLLNQT
                     VTVNDLLEDSLIVKLPYGTS"
BASE COUNT          315 a          215 c          215 g          308 t
ORIGIN      
        1 tggcaatcta ttctaccttt ttacaacgcg cttacgacaa tattatccat gatgtagcgt
       61 tgcaaaattt accggtagta tttgtcctgg atcgaggagg aatcgtcggc gaagatggtc
      121 cgacccatca cggtgctttc gatttgagtt acttatgcac agtgcccaat atgatcgtag
      181 ccgcaccacg caacggcact gaactgcgca atttaatgtt caccgcactc gagcagaacg
      241 atgcgccctt tgccattcgc tatccaaaag attcttatgt cgaatttgac gatcaagcac
      301 cagccgtgct ctaccaaatt ggaaagtggg agataattca taatggtcaa gacgtcgcca
      361 tcttgtcggt cggcgtaatg accaataacg cgatagaggc ggcctgctta cttaaatcaa
      421 aaaatatatt tccgaccatt gtccacgctc gttttgtgaa accacttgat actcaaatgt
      481 tgtcggagat tgccagtaag catcgcatta ttatcacgat tgaagagaac agcattgttg
      541 gtggattcgg ggaacaggtt gccagatatt ttgccgaaca aaacctaccg caaaagatta
      601 acattttggc tataccagac cattttattg aacaaggagc gcgtgaaatt ttactgagag
      661 acttagcgct cgatgccatc ggaattgcga acaaagtcga gcaaatttat cgtcaagctg
      721 attagttata aaacccgggg aaaattatta ataaactatt tatttccatt gaatacgaat
      781 tgttgagact taggtcacat cattttatta tcaattaaaa taaaatcaat cagaaatttt
      841 gaaattaaat gaggatagta atgcaaaagt ttaaaatcat catcatcgcc ggagtggcgg
      901 ttctattttc attttgtgat cttaaaatcc ccagtgaatc ggattttccg acttggtcgg
      961 taagtttgag cattccgcta ttaaatcaga ccgttactgt caatgattta ttagaagatt
     1021 cgctgatagt caagcttcct tatggtacct ctg
//
